dpysl2b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	dpysl2b
Entrez Gene ID	553412
Full Name	dihydropyrimidinase-like 2b
Synonyms	CRMP-2,CRMP2,dpysl2
General protein information	Preferred Names dihydropyrimidinase-like 2b Names dihydropyrimidinase-related protein 2 collapsin response mediator protein 2 dihydropyrimidinase-like 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005155631.4	XP_005155688.1	dihydropyrimidinase-related protein 2 isoform X1
XM_005155631.3	XP_005155688.1	dihydropyrimidinase-related protein 2 isoform X1
NM_001020517.2	NP_001018353.1	dihydropyrimidinase-related protein 2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC798555	XP_003199094.1
Danio rerio (zebrafish)	dpysl2b	NP_001018353.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Evolution and expression of the phosphodiesterase 6 genes unveils vertebrate novelty to control photosensitivity.
Lagman D, Franz?n IE, Eggert J, Larhammar D, Abalo XM
BMC evolutionary biology16(1)124(2016 Jun)

On the crucial cerebellar wound healing-related pathways and their cross-talks after traumatic brain injury in Danio rerio.
Wu CC, Tsai TH, Chang C, Lee TT, Lin C, Cheng IH, Sun MC, Chuang YJ, Chen BS
PloS one9(6)e97902(2014)

Phosphorylation of Dpsyl2 (CRMP2) and Dpsyl3 (CRMP4) is required for positioning of caudal primary motor neurons in the zebrafish spinal cord.
Morimura R, Nozawa K, Tanaka H, Ohshima T
Developmental neurobiology73(12)911-20(2013 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

phosphorylation of Dpysl2 and Dpysl3 by Cyclin-dependent kinase 5 and dual-specificity tyrosine-phosphorylated and regulated kinase 2 is required for correct positioning of CaP motor neurons in the zebrafish spinal cord
Title: Phosphorylation of Dpsyl2 (CRMP2) and Dpsyl3 (CRMP4) is required for positioning of caudal primary motor neurons in the zebrafish spinal cord.

These results suggest that the phosphorylation of Dpysl2 and Dpysl3 by Cdk5 and DYRK2 is required for the proper positioning of Rohon-Beard neurons and neural crest cells during neurulation in zebrafish embryos.
Title: Dpysl2 (CRMP2) and Dpysl3 (CRMP4) phosphorylation by Cdk5 and DYRK2 is required for proper positioning of Rohon-Beard neurons and neural crest cells during neurulation in zebrafish.

From 20 somites through 30 hpf CRMP2 is expressed in the dorsal rostral cluster of the telencephalon, the ventral rostral cluster of the diencephalon, the ventral caudal cluster of the mesencephalon, and the hindbrain clusters.
Title: Zebrafish collapsin response mediator protein (CRMP)-2 is expressed in developing neurons.

The following dpysl2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dpysl2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa32637	NM_001020517.2 Latest version!	Danio rerio dihydropyrimidinase like 2b (dpysl2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa15953	XM_005155631.4 Latest version!	Danio rerio dihydropyrimidinase-like 2b (dpysl2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
View Old Accession Versions >>
ODa15953	XM_005155631.3	Danio rerio dihydropyrimidinase-like 2b (dpysl2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dpysl2b ( NM_001020517.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001018353.1 Danio rerio dihydropyrimidinase like 2b (dpysl2b), mRNA.

Price & Availability↑

CloneID	ODa32637
Clone ID Related Accession (Same CDS sequence)	NM_001020517.2
Accession Version	NM_001020517.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-07
Organism	Danio rerio(zebrafish)
Product	dihydropyrimidinase-related protein 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC129137.1. On Jan 10, 2007 this sequence version replaced NM_001020517.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC129137.1, AY997023.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTCTGGCT ATCAGGGCAA GAAGAATATT CCCCGCATAA CCAGTGACCG TCTCCTGATT 
AAAGGGGCTA AGATTGTGAA CGATGATCAG TCGTTCTGTG CCGACATCTA TATGGAGGAT 
GGCCTGATCA AGCAAATTGG AGAAAATCTA ATAGTGCCCG GTGGGGTGAA AACCATCGAT 
GCCCATGGGC GCATGGTGAT GCCTGGTGGT ATTGATGTGC ACACACGCTT CCAGATGCCT 
GACAGGGGTA TGACTGCTGC TGATGACTTC TACCAAGGCA CCCGTGCTGC TCTGGCTGGA 
GGAACCACCA TTATCGTTGA TCATGTGGTG CCTGAACCCG GGACGAGTTT GATCTCAGCC 
TTCAATCAGT GGAGGGAATG GGCAGACAGT AAAGCATGCT GTGATTATTC TCTTCACGTG 
GACATCACTG AATGGCACAA GGGTGTTCAG GAGGAGATGG AGGCACTCGT CAAAGACCAT 
GGCGTTAACT CTTTCCTCGT CTACCTGGCC TATAAAGACT TTTTCCAGCT CACTGATTCA 
CAGATCTACG AAGTGTTCAG TGTAATTCGG GACCTCGGGG CCATCGCTCA GGTCCACGCT 
GAGAACGGAG ACATCATAGC CGAGGAGCAG CGCAGGATCT TGGACTTGGG TATTACTGGA 
CCAGAGGGAC ATGTCCTCAG TCGTCCAGAG GAGGTGGAGG CGGAGGCAGT GAACCGCTCT 
GTAACAGTCG CCAATCAGAC CAACTGCCCA CTTTACATCA CTAAGGTGAT GAGCAAGAGT 
GCCGCTGATG TCATCGCCCA GGCCAGGAAG AAAGGCACCG TGGTGTATGG AGAGCCTATC 
ACAGCGAGTA TTGGCACGGA TGGATCACAC TATTGGAGCA AGAACTGGGC CAAGGCTGCC 
GCCTACGTCA CGTCTCCACC CTTGAGTCCA GATCCCACCA CACCAGACTA TCTCAACTCA 
CTGCTGTCCT GTGGGGATCT GCAGGTAACA GGAAGTGCTC ACTGCACCTT TAACACATCC 
CAGAGAGCTG TTGGTAAAGA TGACTTCACC CTCATCCCAG AGGGCACCAA TGGCACAGAG 
GAAAGGATGT CCATCATCTG GGACAAATGT GTGGTAACAG GAAAAATGGA TGAAAATCAG 
TTTGTGGCTG TCACCAGCAC CAATGCTGCT AAGATCTTCA ACCTGTATCC GCGCAAGGGC 
CGCATTGCTG TGGGCTCAGA TGCCGATCTT GTTCTGTGGG ACCCTGATGT TACGAAGATC 
ATCTCAGCCA AGAGCCACAA TCTGGCAGTG GAGTACAACA TTTTCGAGGG TATGGAGGTG 
CGTGGAGTGC CCCTGGTGGT CATCAGTCAG GGCAAGATTG TGCTGGAGGA AGGAACCCTG 
CACACCACTG AAGGGTCCGG ACGCTTCATA TCCCGCAAAC CCTTCCCTGA CTACGTTTAC 
AAGAGGATAA AGGCCCGCAG CAGGCTTGCT GAACTGCGGG GCGTCCCGCG GGGTCTGTAC 
GACGGGCCGG TCTGTGAAGT GTCAGTGACG CCCAAAGCTG TGACGCCTGC TTCCTCTGCT 
AAAACCTCTC CTGCCAAGCA GCCCGGGCAA CCCGTCCGCA ACCTGCACCA GTCCGGCTTC 
AGCCTCTCTG GTGCTCAAGT TGATGACAAC ATCCCTCGCC GCAACACCCA ACGCATTGTG 
GCACCCCCTG GTGGCAGAGC CAACATCACC AGCCTGGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001018353.1
CDS	156..1877
Translation	MSGYQGKKNIPRITSDRLLIKGAKIVNDDQSFCADIYMEDGLIKQIGENLIVPGGVKTIDAHGRMVMPGGIDVHTRFQMPDRGMTAADDFYQGTRAALAGGTTIIVDHVVPEPGTSLISAFNQWREWADSKACCDYSLHVDITEWHKGVQEEMEALVKDHGVNSFLVYLAYKDFFQLTDSQIYEVFSVIRDLGAIAQVHAENGDIIAEEQRRILDLGITGPEGHVLSRPEEVEAEAVNRSVTVANQTNCPLYITKVMSKSAADVIAQARKKGTVVYGEPITASIGTDGSHYWSKNWAKAAAYVTSPPLSPDPTTPDYLNSLLSCGDLQVTGSAHCTFNTSQRAVGKDDFTLIPEGTNGTEERMSIIWDKCVVTGKMDENQFVAVTSTNAAKIFNLYPRKGRIAVGSDADLVLWDPDVTKIISAKSHNLAVEYNIFEGMEVRGVPLVVISQGKIVLEEGTLHTTEGSGRFISRKPFPDYVYKRIKARSRLAELRGVPRGLYDGPVCEVSVTPKAVTPASSAKTSPAKQPGQPVRNLHQSGFSLSGAQVDDNIPRRNTQRIVAPPGGRANITSLG

Target ORF information:

RefSeq Version	NM_001020517.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio dihydropyrimidinase like 2b (dpysl2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001020517.2

ORF Insert Sequence:

ATGTCTGGCT ATCAGGGCAA GAAGAATATT CCCCGCATAA CCAGTGACCG TCTCCTGATT 
AAAGGGGCTA AGATTGTGAA CGATGATCAG TCGTTCTGTG CCGACATCTA TATGGAGGAT 
GGCCTGATCA AGCAAATTGG AGAAAATCTA ATAGTGCCCG GTGGGGTGAA AACCATCGAT 
GCCCATGGGC GCATGGTGAT GCCTGGTGGT ATTGATGTGC ACACACGCTT CCAGATGCCT 
GACAGGGGTA TGACTGCTGC TGATGACTTC TACCAAGGCA CCCGTGCTGC TCTGGCTGGA 
GGAACCACCA TTATCGTTGA TCATGTGGTG CCTGAACCCG GGACGAGTTT GATCTCAGCC 
TTCAATCAGT GGAGGGAATG GGCAGACAGT AAAGCATGCT GTGATTATTC TCTTCACGTG 
GACATCACTG AATGGCACAA GGGTGTTCAG GAGGAGATGG AGGCACTCGT CAAAGACCAT 
GGCGTTAACT CTTTCCTCGT CTACCTGGCC TATAAAGACT TTTTCCAGCT CACTGATTCA 
CAGATCTACG AAGTGTTCAG TGTAATTCGG GACCTCGGGG CCATCGCTCA GGTCCACGCT 
GAGAACGGAG ACATCATAGC CGAGGAGCAG CGCAGGATCT TGGACTTGGG TATTACTGGA 
CCAGAGGGAC ATGTCCTCAG TCGTCCAGAG GAGGTGGAGG CGGAGGCAGT GAACCGCTCT 
GTAACAGTCG CCAATCAGAC CAACTGCCCA CTTTACATCA CTAAGGTGAT GAGCAAGAGT 
GCCGCTGATG TCATCGCCCA GGCCAGGAAG AAAGGCACCG TGGTGTATGG AGAGCCTATC 
ACAGCGAGTA TTGGCACGGA TGGATCACAC TATTGGAGCA AGAACTGGGC CAAGGCTGCC 
GCCTACGTCA CGTCTCCACC CTTGAGTCCA GATCCCACCA CACCAGACTA TCTCAACTCA 
CTGCTGTCCT GTGGGGATCT GCAGGTAACA GGAAGTGCTC ACTGCACCTT TAACACATCC 
CAGAGAGCTG TTGGTAAAGA TGACTTCACC CTCATCCCAG AGGGCACCAA TGGCACAGAG 
GAAAGGATGT CCATCATCTG GGACAAATGT GTGGTAACAG GAAAAATGGA TGAAAATCAG 
TTTGTGGCTG TCACCAGCAC CAATGCTGCT AAGATCTTCA ACCTGTATCC GCGCAAGGGC 
CGCATTGCTG TGGGCTCAGA TGCCGATCTT GTTCTGTGGG ACCCTGATGT TACGAAGATC 
ATCTCAGCCA AGAGCCACAA TCTGGCAGTG GAGTACAACA TTTTCGAGGG TATGGAGGTG 
CGTGGAGTGC CCCTGGTGGT CATCAGTCAG GGCAAGATTG TGCTGGAGGA AGGAACCCTG 
CACACCACTG AAGGGTCCGG ACGCTTCATA TCCCGCAAAC CCTTCCCTGA CTACGTTTAC 
AAGAGGATAA AGGCCCGCAG CAGGCTTGCT GAACTGCGGG GCGTCCCGCG GGGTCTGTAC 
GACGGGCCGG TCTGTGAAGT GTCAGTGACG CCCAAAGCTG TGACGCCTGC TTCCTCTGCT 
AAAACCTCTC CTGCCAAGCA GCCCGGGCAA CCCGTCCGCA ACCTGCACCA GTCCGGCTTC 
AGCCTCTCTG GTGCTCAAGT TGATGACAAC ATCCCTCGCC GCAACACCCA ACGCATTGTG 
GCACCCCCTG GTGGCAGAGC CAACATCACC AGCCTGGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dpysl2b ( XM_005155631.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155688.1 Danio rerio dihydropyrimidinase-like 2b (dpysl2b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa15953
Clone ID Related Accession (Same CDS sequence)	XM_005155631.4 , XM_005155631.3
Accession Version	XM_005155631.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	dihydropyrimidinase-related protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005155631.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTG CCGGAGATGT GCCTGCCTTC TTCCACAACA TGGGAAGCTC GAGCCCCAAC 
CAGCCCCGGC AGAAATTCTG TGGCATGTTC TGCCCCGTGG AGGGTTCATC GGATAGCAAG 
ACGTTGGACT TTGACTCCCT GTCCTCAGGA AGAGGTGGAG TGATCGAGAG GCAGAGGGAC 
ATCTCCAAAG CGAGAAAGGT GGAGATCAGA AGGAGCGCTG GAAAGGAGCT TCTGCAGAAC 
CTGAATGATG AGAGTGACCG TCTCCTGATT AAAGGGGCTA AGATTGTGAA CGATGATCAG 
TCGTTCTGTG CCGACATCTA TATGGAGGAT GGCCTGATCA AGCAAATTGG AGAAAATCTA 
ATAGTGCCCG GTGGGGTGAA AACCATCGAT GCCCATGGGC GCATGGTGAT GCCTGGTGGT 
ATTGATGTGC ACACACGCTT CCAGATGCCT GACAGGGGTA TGACTGCTGC TGATGACTTC 
TACCAAGGCA CCCGTGCTGC TCTGGCTGGA GGAACCACCA TGATCGTTGA TCATGTGGTG 
CCTGAACCCG GGACGAGTTT GATCTCAGCC TTCAATCAGT GGAGGGAATG GGCAGACAGT 
AAAGCATGCT GTGATTATTC TCTTCACGTG GACATCACTG AATGGCACAA GGGTGTTCAG 
GAGGAGATGG AGGCACTCGT CAAAGACCAT GGCGTTAACT CTTTCCTCGT CTACCTGGCC 
TATAAAGACT TTTTCCAGCT CACTGATTCA CAGATCTACG AAGTGTTCAG TGTAATTCGG 
GACCTCGGGG CCATCGCTCA GGTCCACGCT GAGAACGGAG ACATCATAGC CGAGGAGCAG 
CGCAGGATCT TGGACTTGGG TATTACTGGA CCAGAGGGAC ATGTCCTCAG TCGTCCAGAG 
GAGGTGGAGG CGGAGGCAGT GAACCGCTCT GTAACAGTCG CCAATCAGAC CAACTGCCCA 
CTTTACATCA CTAAGGTGAT GAGCAAGAGT GCCGCTGATG TCATCGCCCA GGCCAGGAAG 
AAAGGCACCG TGGTGTATGG AGAGCCTATC ACAGCGAGTA TTGGCACGGA TGGATCACAC 
TATTGGAGCA AGAACTGGGC CAAGGCTGCC GCCTACGTCA CGTCTCCACC CTTGAGTCCA 
GATCCCACCA CACCAGACTA TCTCAACTCA CTGCTGTCCT GTGGGGATCT GCAGGTAACA 
GGAAGTGCTC ACTGCACCTT TAACACATCC CAGAGAGCTG TTGGTAAAGA TGACTTCACC 
CTCATCCCAG AGGGCACCAA TGGCACAGAG GAAAGGATGT CCATCATCTG GGACAAATGT 
GTGGTAACAG GAAAAATGGA TGAAAATCAG TTTGTGGCTG TCACCAGCAC CAATGCTGCT 
AAGATCTTCA ACCTGTATCC GCGCAAGGGC CGCATTGCTG TGGGCTCAGA TGCCGATCTT 
GTTCTGTGGG ACCCTGATGT TACGAAGATC ATCTCAGCCA AGAGCCACAA TCTGGCAGTG 
GAGTACAACA TTTTCGAGGG TATGGAGGTG CGTGGAGTGC CCCTGGTGGT CATCAGTCAG 
GGCAAGATTG TGCTGGAGGA AGGAACCCTG CACACCACTG AAGGGTCCGG ACGCTTCATA 
TCCCGCAAAC CCTTCCCTGA CTACGTTTAC AAGAGGATAA AGGCCCGCAG CAGGCTTGCT 
GAACTGCGGG GCGTCCCGCG GGGTCTGTAC GACGGGCCGG TCTGTGAAGT GTCAGTGACG 
CCCAAAGCTG TGACGCCTGC TTCCTCTGCT AAAACCTCTC CTGCCAAGCA GCCCGGGCAA 
CCCGTCCGCA ACCTGCACCA GTCCGGCTTC AGCCTCTCTG GTGCTCAAGT TGATGACAAC 
ATCCCTCGCC GCAACACCCA ACGCATTGTG GCACCCCCTG GTGGCAGAGC CAACATCACC 
AGCCTGGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155688.1
CDS	302..2233
Translation	MSAAGDVPAFFHNMGSSSPNQPRQKFCGMFCPVEGSSDSKTLDFDSLSSGRGGVIERQRDISKARKVEIRRSAGKELLQNLNDESDRLLIKGAKIVNDDQSFCADIYMEDGLIKQIGENLIVPGGVKTIDAHGRMVMPGGIDVHTRFQMPDRGMTAADDFYQGTRAALAGGTTMIVDHVVPEPGTSLISAFNQWREWADSKACCDYSLHVDITEWHKGVQEEMEALVKDHGVNSFLVYLAYKDFFQLTDSQIYEVFSVIRDLGAIAQVHAENGDIIAEEQRRILDLGITGPEGHVLSRPEEVEAEAVNRSVTVANQTNCPLYITKVMSKSAADVIAQARKKGTVVYGEPITASIGTDGSHYWSKNWAKAAAYVTSPPLSPDPTTPDYLNSLLSCGDLQVTGSAHCTFNTSQRAVGKDDFTLIPEGTNGTEERMSIIWDKCVVTGKMDENQFVAVTSTNAAKIFNLYPRKGRIAVGSDADLVLWDPDVTKIISAKSHNLAVEYNIFEGMEVRGVPLVVISQGKIVLEEGTLHTTEGSGRFISRKPFPDYVYKRIKARSRLAELRGVPRGLYDGPVCEVSVTPKAVTPASSAKTSPAKQPGQPVRNLHQSGFSLSGAQVDDNIPRRNTQRIVAPPGGRANITSLG

Target ORF information:

RefSeq Version	XM_005155631.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio dihydropyrimidinase-like 2b (dpysl2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155631.4

ORF Insert Sequence:

ATGTCTGCTG CCGGAGATGT GCCTGCCTTC TTCCACAACA TGGGAAGCTC GAGCCCCAAC 
CAGCCCCGGC AGAAATTCTG TGGCATGTTC TGCCCCGTGG AGGGTTCATC GGATAGCAAG 
ACGTTGGACT TTGACTCCCT GTCCTCAGGA AGAGGTGGAG TGATCGAGAG GCAGAGGGAC 
ATCTCCAAAG CGAGAAAGGT GGAGATCAGA AGGAGCGCTG GAAAGGAGCT TCTGCAGAAC 
CTGAATGATG AGAGTGACCG TCTCCTGATT AAAGGGGCTA AGATTGTGAA CGATGATCAG 
TCGTTCTGTG CCGACATCTA TATGGAGGAT GGCCTGATCA AGCAAATTGG AGAAAATCTA 
ATAGTGCCCG GTGGGGTGAA AACCATCGAT GCCCATGGGC GCATGGTGAT GCCTGGTGGT 
ATTGATGTGC ACACACGCTT CCAGATGCCT GACAGGGGTA TGACTGCTGC TGATGACTTC 
TACCAAGGCA CCCGTGCTGC TCTGGCTGGA GGAACCACCA TGATCGTTGA TCATGTGGTG 
CCTGAACCCG GGACGAGTTT GATCTCAGCC TTCAATCAGT GGAGGGAATG GGCAGACAGT 
AAAGCATGCT GTGATTATTC TCTTCACGTG GACATCACTG AATGGCACAA GGGTGTTCAG 
GAGGAGATGG AGGCACTCGT CAAAGACCAT GGCGTTAACT CTTTCCTCGT CTACCTGGCC 
TATAAAGACT TTTTCCAGCT CACTGATTCA CAGATCTACG AAGTGTTCAG TGTAATTCGG 
GACCTCGGGG CCATCGCTCA GGTCCACGCT GAGAACGGAG ACATCATAGC CGAGGAGCAG 
CGCAGGATCT TGGACTTGGG TATTACTGGA CCAGAGGGAC ATGTCCTCAG TCGTCCAGAG 
GAGGTGGAGG CGGAGGCAGT GAACCGCTCT GTAACAGTCG CCAATCAGAC CAACTGCCCA 
CTTTACATCA CTAAGGTGAT GAGCAAGAGT GCCGCTGATG TCATCGCCCA GGCCAGGAAG 
AAAGGCACCG TGGTGTATGG AGAGCCTATC ACAGCGAGTA TTGGCACGGA TGGATCACAC 
TATTGGAGCA AGAACTGGGC CAAGGCTGCC GCCTACGTCA CGTCTCCACC CTTGAGTCCA 
GATCCCACCA CACCAGACTA TCTCAACTCA CTGCTGTCCT GTGGGGATCT GCAGGTAACA 
GGAAGTGCTC ACTGCACCTT TAACACATCC CAGAGAGCTG TTGGTAAAGA TGACTTCACC 
CTCATCCCAG AGGGCACCAA TGGCACAGAG GAAAGGATGT CCATCATCTG GGACAAATGT 
GTGGTAACAG GAAAAATGGA TGAAAATCAG TTTGTGGCTG TCACCAGCAC CAATGCTGCT 
AAGATCTTCA ACCTGTATCC GCGCAAGGGC CGCATTGCTG TGGGCTCAGA TGCCGATCTT 
GTTCTGTGGG ACCCTGATGT TACGAAGATC ATCTCAGCCA AGAGCCACAA TCTGGCAGTG 
GAGTACAACA TTTTCGAGGG TATGGAGGTG CGTGGAGTGC CCCTGGTGGT CATCAGTCAG 
GGCAAGATTG TGCTGGAGGA AGGAACCCTG CACACCACTG AAGGGTCCGG ACGCTTCATA 
TCCCGCAAAC CCTTCCCTGA CTACGTTTAC AAGAGGATAA AGGCCCGCAG CAGGCTTGCT 
GAACTGCGGG GCGTCCCGCG GGGTCTGTAC GACGGGCCGG TCTGTGAAGT GTCAGTGACG 
CCCAAAGCTG TGACGCCTGC TTCCTCTGCT AAAACCTCTC CTGCCAAGCA GCCCGGGCAA 
CCCGTCCGCA ACCTGCACCA GTCCGGCTTC AGCCTCTCTG GTGCTCAAGT TGATGACAAC 
ATCCCTCGCC GCAACACCCA ACGCATTGTG GCACCCCCTG GTGGCAGAGC CAACATCACC 
AGCCTGGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dpysl2b ( XM_005155631.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155688.1 Danio rerio dihydropyrimidinase-like 2b (dpysl2b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa15953
Clone ID Related Accession (Same CDS sequence)	XM_005155631.4 , XM_005155631.3
Accession Version	XM_005155631.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	dihydropyrimidinase-related protein 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005155631.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTG CCGGAGATGT GCCTGCCTTC TTCCACAACA TGGGAAGCTC GAGCCCCAAC 
CAGCCCCGGC AGAAATTCTG TGGCATGTTC TGCCCCGTGG AGGGTTCATC GGATAGCAAG 
ACGTTGGACT TTGACTCCCT GTCCTCAGGA AGAGGTGGAG TGATCGAGAG GCAGAGGGAC 
ATCTCCAAAG CGAGAAAGGT GGAGATCAGA AGGAGCGCTG GAAAGGAGCT TCTGCAGAAC 
CTGAATGATG AGAGTGACCG TCTCCTGATT AAAGGGGCTA AGATTGTGAA CGATGATCAG 
TCGTTCTGTG CCGACATCTA TATGGAGGAT GGCCTGATCA AGCAAATTGG AGAAAATCTA 
ATAGTGCCCG GTGGGGTGAA AACCATCGAT GCCCATGGGC GCATGGTGAT GCCTGGTGGT 
ATTGATGTGC ACACACGCTT CCAGATGCCT GACAGGGGTA TGACTGCTGC TGATGACTTC 
TACCAAGGCA CCCGTGCTGC TCTGGCTGGA GGAACCACCA TGATCGTTGA TCATGTGGTG 
CCTGAACCCG GGACGAGTTT GATCTCAGCC TTCAATCAGT GGAGGGAATG GGCAGACAGT 
AAAGCATGCT GTGATTATTC TCTTCACGTG GACATCACTG AATGGCACAA GGGTGTTCAG 
GAGGAGATGG AGGCACTCGT CAAAGACCAT GGCGTTAACT CTTTCCTCGT CTACCTGGCC 
TATAAAGACT TTTTCCAGCT CACTGATTCA CAGATCTACG AAGTGTTCAG TGTAATTCGG 
GACCTCGGGG CCATCGCTCA GGTCCACGCT GAGAACGGAG ACATCATAGC CGAGGAGCAG 
CGCAGGATCT TGGACTTGGG TATTACTGGA CCAGAGGGAC ATGTCCTCAG TCGTCCAGAG 
GAGGTGGAGG CGGAGGCAGT GAACCGCTCT GTAACAGTCG CCAATCAGAC CAACTGCCCA 
CTTTACATCA CTAAGGTGAT GAGCAAGAGT GCCGCTGATG TCATCGCCCA GGCCAGGAAG 
AAAGGCACCG TGGTGTATGG AGAGCCTATC ACAGCGAGTA TTGGCACGGA TGGATCACAC 
TATTGGAGCA AGAACTGGGC CAAGGCTGCC GCCTACGTCA CGTCTCCACC CTTGAGTCCA 
GATCCCACCA CACCAGACTA TCTCAACTCA CTGCTGTCCT GTGGGGATCT GCAGGTAACA 
GGAAGTGCTC ACTGCACCTT TAACACATCC CAGAGAGCTG TTGGTAAAGA TGACTTCACC 
CTCATCCCAG AGGGCACCAA TGGCACAGAG GAAAGGATGT CCATCATCTG GGACAAATGT 
GTGGTAACAG GAAAAATGGA TGAAAATCAG TTTGTGGCTG TCACCAGCAC CAATGCTGCT 
AAGATCTTCA ACCTGTATCC GCGCAAGGGC CGCATTGCTG TGGGCTCAGA TGCCGATCTT 
GTTCTGTGGG ACCCTGATGT TACGAAGATC ATCTCAGCCA AGAGCCACAA TCTGGCAGTG 
GAGTACAACA TTTTCGAGGG TATGGAGGTG CGTGGAGTGC CCCTGGTGGT CATCAGTCAG 
GGCAAGATTG TGCTGGAGGA AGGAACCCTG CACACCACTG AAGGGTCCGG ACGCTTCATA 
TCCCGCAAAC CCTTCCCTGA CTACGTTTAC AAGAGGATAA AGGCCCGCAG CAGGCTTGCT 
GAACTGCGGG GCGTCCCGCG GGGTCTGTAC GACGGGCCGG TCTGTGAAGT GTCAGTGACG 
CCCAAAGCTG TGACGCCTGC TTCCTCTGCT AAAACCTCTC CTGCCAAGCA GCCCGGGCAA 
CCCGTCCGCA ACCTGCACCA GTCCGGCTTC AGCCTCTCTG GTGCTCAAGT TGATGACAAC 
ATCCCTCGCC GCAACACCCA ACGCATTGTG GCACCCCCTG GTGGCAGAGC CAACATCACC 
AGCCTGGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155688.1
CDS	249..2180
Translation	MSAAGDVPAFFHNMGSSSPNQPRQKFCGMFCPVEGSSDSKTLDFDSLSSGRGGVIERQRDISKARKVEIRRSAGKELLQNLNDESDRLLIKGAKIVNDDQSFCADIYMEDGLIKQIGENLIVPGGVKTIDAHGRMVMPGGIDVHTRFQMPDRGMTAADDFYQGTRAALAGGTTMIVDHVVPEPGTSLISAFNQWREWADSKACCDYSLHVDITEWHKGVQEEMEALVKDHGVNSFLVYLAYKDFFQLTDSQIYEVFSVIRDLGAIAQVHAENGDIIAEEQRRILDLGITGPEGHVLSRPEEVEAEAVNRSVTVANQTNCPLYITKVMSKSAADVIAQARKKGTVVYGEPITASIGTDGSHYWSKNWAKAAAYVTSPPLSPDPTTPDYLNSLLSCGDLQVTGSAHCTFNTSQRAVGKDDFTLIPEGTNGTEERMSIIWDKCVVTGKMDENQFVAVTSTNAAKIFNLYPRKGRIAVGSDADLVLWDPDVTKIISAKSHNLAVEYNIFEGMEVRGVPLVVISQGKIVLEEGTLHTTEGSGRFISRKPFPDYVYKRIKARSRLAELRGVPRGLYDGPVCEVSVTPKAVTPASSAKTSPAKQPGQPVRNLHQSGFSLSGAQVDDNIPRRNTQRIVAPPGGRANITSLG

Target ORF information:

RefSeq Version	XM_005155631.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio dihydropyrimidinase-like 2b (dpysl2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155631.3

ORF Insert Sequence:

ATGTCTGCTG CCGGAGATGT GCCTGCCTTC TTCCACAACA TGGGAAGCTC GAGCCCCAAC 
CAGCCCCGGC AGAAATTCTG TGGCATGTTC TGCCCCGTGG AGGGTTCATC GGATAGCAAG 
ACGTTGGACT TTGACTCCCT GTCCTCAGGA AGAGGTGGAG TGATCGAGAG GCAGAGGGAC 
ATCTCCAAAG CGAGAAAGGT GGAGATCAGA AGGAGCGCTG GAAAGGAGCT TCTGCAGAAC 
CTGAATGATG AGAGTGACCG TCTCCTGATT AAAGGGGCTA AGATTGTGAA CGATGATCAG 
TCGTTCTGTG CCGACATCTA TATGGAGGAT GGCCTGATCA AGCAAATTGG AGAAAATCTA 
ATAGTGCCCG GTGGGGTGAA AACCATCGAT GCCCATGGGC GCATGGTGAT GCCTGGTGGT 
ATTGATGTGC ACACACGCTT CCAGATGCCT GACAGGGGTA TGACTGCTGC TGATGACTTC 
TACCAAGGCA CCCGTGCTGC TCTGGCTGGA GGAACCACCA TGATCGTTGA TCATGTGGTG 
CCTGAACCCG GGACGAGTTT GATCTCAGCC TTCAATCAGT GGAGGGAATG GGCAGACAGT 
AAAGCATGCT GTGATTATTC TCTTCACGTG GACATCACTG AATGGCACAA GGGTGTTCAG 
GAGGAGATGG AGGCACTCGT CAAAGACCAT GGCGTTAACT CTTTCCTCGT CTACCTGGCC 
TATAAAGACT TTTTCCAGCT CACTGATTCA CAGATCTACG AAGTGTTCAG TGTAATTCGG 
GACCTCGGGG CCATCGCTCA GGTCCACGCT GAGAACGGAG ACATCATAGC CGAGGAGCAG 
CGCAGGATCT TGGACTTGGG TATTACTGGA CCAGAGGGAC ATGTCCTCAG TCGTCCAGAG 
GAGGTGGAGG CGGAGGCAGT GAACCGCTCT GTAACAGTCG CCAATCAGAC CAACTGCCCA 
CTTTACATCA CTAAGGTGAT GAGCAAGAGT GCCGCTGATG TCATCGCCCA GGCCAGGAAG 
AAAGGCACCG TGGTGTATGG AGAGCCTATC ACAGCGAGTA TTGGCACGGA TGGATCACAC 
TATTGGAGCA AGAACTGGGC CAAGGCTGCC GCCTACGTCA CGTCTCCACC CTTGAGTCCA 
GATCCCACCA CACCAGACTA TCTCAACTCA CTGCTGTCCT GTGGGGATCT GCAGGTAACA 
GGAAGTGCTC ACTGCACCTT TAACACATCC CAGAGAGCTG TTGGTAAAGA TGACTTCACC 
CTCATCCCAG AGGGCACCAA TGGCACAGAG GAAAGGATGT CCATCATCTG GGACAAATGT 
GTGGTAACAG GAAAAATGGA TGAAAATCAG TTTGTGGCTG TCACCAGCAC CAATGCTGCT 
AAGATCTTCA ACCTGTATCC GCGCAAGGGC CGCATTGCTG TGGGCTCAGA TGCCGATCTT 
GTTCTGTGGG ACCCTGATGT TACGAAGATC ATCTCAGCCA AGAGCCACAA TCTGGCAGTG 
GAGTACAACA TTTTCGAGGG TATGGAGGTG CGTGGAGTGC CCCTGGTGGT CATCAGTCAG 
GGCAAGATTG TGCTGGAGGA AGGAACCCTG CACACCACTG AAGGGTCCGG ACGCTTCATA 
TCCCGCAAAC CCTTCCCTGA CTACGTTTAC AAGAGGATAA AGGCCCGCAG CAGGCTTGCT 
GAACTGCGGG GCGTCCCGCG GGGTCTGTAC GACGGGCCGG TCTGTGAAGT GTCAGTGACG 
CCCAAAGCTG TGACGCCTGC TTCCTCTGCT AAAACCTCTC CTGCCAAGCA GCCCGGGCAA 
CCCGTCCGCA ACCTGCACCA GTCCGGCTTC AGCCTCTCTG GTGCTCAAGT TGATGACAAC 
ATCCCTCGCC GCAACACCCA ACGCATTGTG GCACCCCCTG GTGGCAGAGC CAACATCACC 
AGCCTGGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Evolution and expression of the phosphodiesterase 6 genes unveils vertebrate novelty to control photosensitivity.
BMC evolutionary biology16(1)124(2016 Jun)
Lagman D,Franz?n IE,Eggert J,Larhammar D,Abalo XM

On the crucial cerebellar wound healing-related pathways and their cross-talks after traumatic brain injury in Danio rerio.
PloS one9(6)e97902(2014)
Wu CC,Tsai TH,Chang C,Lee TT,Lin C,Cheng IH,Sun MC,Chuang YJ,Chen BS

Phosphorylation of Dpsyl2 (CRMP2) and Dpsyl3 (CRMP4) is required for positioning of caudal primary motor neurons in the zebrafish spinal cord.
Developmental neurobiology73(12)911-20(2013 Dec)
Morimura R,Nozawa K,Tanaka H,Ohshima T

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dpysl2b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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