dgkza cDNA ORF clone, Danio rerio(zebrafish)

The following dgkza gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dgkza cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa143498 XM_021477928.1
Latest version!
Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa143499 XM_021477929.1
Latest version!
Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa13277 XM_005169029.4
Latest version!
Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa13278 XM_005169030.3
Latest version!
Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa13279 XM_005169031.3
Latest version!
Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa13280 XM_005169032.3
Latest version!
Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa13281 XM_005169033.4
Latest version!
Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa13282 XM_005169034.4
Latest version!
Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa13283 XM_005169035.3
Latest version!
Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa13284 XM_001922410.6
Latest version!
Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa13285 XM_005169036.3
Latest version!
Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa13286 XM_005169037.4
Latest version!
Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa41929 XM_009303361.2
Latest version!
Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa41930 XM_009303362.3
Latest version!
Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
ODa13286 XM_005169037.3 Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa13281 XM_005169033.3 Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa13282 XM_005169034.3 Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa41930 XM_009303362.2 Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa13284 XM_001922410.5 Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa13277 XM_005169029.3 Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa143498
    Clone ID Related Accession (Same CDS sequence) XM_021477928.1
    Accession Version XM_021477928.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3885bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product diacylglycerol kinase zeta isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
    CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT
    TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG
    CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA
    GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT
    CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC
    CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC
    CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA
    TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA
    CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC
    CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT
    GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG
    ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC
    AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC
    CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA
    ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG
    ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG
    CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC
    ATTGAACAGC TGGAGAAGTT ATTGGATTGG AATGCTCCAC CTCAGGGGGA GGGGCTTGAT
    GAGATGCCTC TCAAAGAGCC GTCATTCAGA ATAAACTTCA GATGTAAACC GTCTTTCAGG
    GAGTCAGGAT CACGGAACAT CCGAGAGCAA ACCTGGCGAC GCAGAGCTTT CAGAGGTCGC
    TGTGCTTTGG AGCAAATGAG AGGTGCTGCA GCTAAATGTA CTGCGCACAC CTCTAACACA
    CAGCCTGCAA TAGTGCGACA CCACTGGGTC CATCGGAGAA GACAAGAGGG CAAATGCAAA
    CAGTGTGGAA AGGGGTTTCA GCAGAAATTT GCCTTTCACA GTAAAGAGAT TGTAGCCATC
    AGCTGCTCCT GGTGCAAACA AGCATATCAT AATAAGGTGA CATGCTTTAT GCTGCAGCAG
    ATAGAAGAAC CATGTTCATT AGGAGCTCAT GCCGCTGTAA TAGTACCTCC TACTTGGATC
    ATACGGGTTC GCCGACCTCA GTCCTCACTA AAGTCAAGCA AAAAGAAAAA ACGGACATCT
    TTCAAACGTA AATCCAGCAA GAAAGGAGCA GAGGAGGCAC GATGGAAGCC TTTTATAGTG
    AGACCCATTC CCTCCCAACT CATGAAGCCA CTGCTAGTGT TTGTAAATCC AAAGAGTGGA
    GGAAACCAGG GAGCTAAAAT CATCCAGACC TTTCTTTGGT ATCTAAACCC TCGGCAGGTG
    TTTGATCTAA GTCAAGGAGG TCCTCAGGAA GGCTTGGAAA TGTATCGCAA GGTTCACAAC
    TTGAGAATTT TGGCCTGTGG GGGGGATGGC ACGGTGGGCT GGATCTTATC TGCACTTGAT
    CAGCTGCAAT TAAACCCTTC TCCTGCTGTG GCCGTACTTC CCCTGGGCAC AGGAAATGAC
    CTTGCTAGGA CGCTAAACTG GGGTGGGGGC TACACAGATG AACCTCTGAG TAAAATCCTG
    TCTCATGTGG AGGATGGGAA TATTGTACAG CTGGATCGGT GGAATCTTGT TGTGAAGCCC
    AACCCAGAGG CAGGACCAGA GGAAAGAGAC GAGCAGGTGA CGGACAAGCT CCCTCTAGAC
    GTCTTCAATA ACTACTTCAG CCTTGGATTT GATGCTCATG TGACTTTAGA GTTCCACGAA
    TCTCGAGAGG CTAATCCTGA GAAATTTAAC AGCCGGTTCA GAAACAAAAT GTTCTATGCA
    GGGACAGCGT TTTCTGATTT CTTGATGGGC AGCTCCAAAG ACCTGGCTAA GCACATCAAA
    GTAGTCTGTG ACGGCACAGA TTTAACCTCA AAGGTCCAGG ATTTGAAGCT GCAATGCCTG
    GTCTTCCTAA ATATCCCCAG GTACTGTGCG GGCACAATGC CATGGGGCAA TCCGAGTGAG
    CATCATGACT TTGAGCCTCA GCGTCATGAT GATGGCTGCA TTGAGGTTAT TGGATTCACA
    ATGACATCAC TGGCCACACT GCAGGTCGGA GGTCACGGTG AGAGGTTAAA TCAGTGTCGC
    GAAGTCACCC TCACCACCTT TAAGTCAATC CCCATGCAGG TGGACGGGGA GCCTTGTAAA
    CTAGCACCAT CTGTTATTCA CATTTCTTTG CGAAACCAGG CCAACATGGT GCAGAAAACC
    AAGAGACGTA CATCTATACC ACTGCTAAAC GACAGTCAGC AACCGTTTCC AGAACGACTA
    CGGATCAGAG TGAGCCGTAT CAGCATGCAT GATTATGAAG CTTTGCACTA CGACAAGGAG
    AAGCTCAAAG AAGCTTCGAT TCCACTGGGT CTCATCGTTG TCCCTGGAGA CAGTGATTTG
    GAAACCTGCC GAGCTCACAT CGAGAGACTA CAGGAGGACT TTGTGTCGTG TAACCCGTCT
    CTGCAGCGGC TGGTGCTCCA GGAAGGTGAC GGGAGCAAAT CAAAGGCTTT GTCGTCTCAG
    AGGCTATCAC CGAAATGGTG CTTTCTCGAC TCTACAACTG CTGATCGATT CTACAGGATT
    GATCGAGCTC AAGAGCATCT CAACTACGTG ACTGAAATTT CTCAGGATGA GCTGTTTGTT
    TTGGACCCAG AGCTGGTGGT CACAGAGACT GTTAGCACAT CACCTGGCAT GCCAGACCTG
    GTGGACTCTG GTGAAGTCCA GTCCGATCCA CACAAATTTG CCTTTCCGTC ATCCTCTTCT
    TCACCACCAA ACACACCTGG ACCTAGGGTT GCAGAGCTGC AAAGGAAGCG TGTTTCCAGT
    GACAGTTCGG TGGCAGAAGC ACTTGCACAC AGTGACTCGC TCAGATCAGC CAAGCCAGCT
    CTCTGCAGAG CTGGGGGTGT TCATCGCTCA AATACAACCG CAGCAGATTT CAAACCTACC
    AGCAGGTTTG AGAAGAGCGC TGTGAACAAT GCCAACTCTG TGTCAGTGGA CAGTCTAATT
    GAATGTGTGA AAAAGAAGGA CCATCAGAAG TTGAAGGAGC TGCATAAGAA AGGGGCCGAC
    CTGTGTGTGC AGGACCCTGC TGGACGGACC CTATTGCACT ATGCTGTGGA AGTGGGCAGC
    AAGGAGATTG TCAAATACAT CATTGATAAT GCACCCACCG ATATCCTGGA TGTAACAGAG
    AAAGAAAATG GAGAGACTGT GTTGCACAAA GCAGCCTCGC TGTGTCATAG GACAATCTGT
    CATTACCTAG TGGAAGCAGG AGCATCTCTT ATGAAGACAG ACTTACAGGG TGACACTCCG
    AAGCATCGTG CCGAGAAGGC GAAAGATGCT GAACTTGCGG CATATCTTGA AAACCGCCAG
    CACTACCAAA TGATCCAGCG TGAGGACCAG GAGACTGCAG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333603.1
    CDS172..4056
    Translation

    Target ORF information:

    RefSeq Version XM_021477928.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021477928.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
    CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT
    TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG
    CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA
    GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT
    CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC
    CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC
    CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA
    TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA
    CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC
    CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT
    GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG
    ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC
    AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC
    CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA
    ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG
    ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG
    CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC
    ATTGAACAGC TGGAGAAGTT ATTGGATTGG AATGCTCCAC CTCAGGGGGA GGGGCTTGAT
    GAGATGCCTC TCAAAGAGCC GTCATTCAGA ATAAACTTCA GATGTAAACC GTCTTTCAGG
    GAGTCAGGAT CACGGAACAT CCGAGAGCAA ACCTGGCGAC GCAGAGCTTT CAGAGGTCGC
    TGTGCTTTGG AGCAAATGAG AGGTGCTGCA GCTAAATGTA CTGCGCACAC CTCTAACACA
    CAGCCTGCAA TAGTGCGACA CCACTGGGTC CATCGGAGAA GACAAGAGGG CAAATGCAAA
    CAGTGTGGAA AGGGGTTTCA GCAGAAATTT GCCTTTCACA GTAAAGAGAT TGTAGCCATC
    AGCTGCTCCT GGTGCAAACA AGCATATCAT AATAAGGTGA CATGCTTTAT GCTGCAGCAG
    ATAGAAGAAC CATGTTCATT AGGAGCTCAT GCCGCTGTAA TAGTACCTCC TACTTGGATC
    ATACGGGTTC GCCGACCTCA GTCCTCACTA AAGTCAAGCA AAAAGAAAAA ACGGACATCT
    TTCAAACGTA AATCCAGCAA GAAAGGAGCA GAGGAGGCAC GATGGAAGCC TTTTATAGTG
    AGACCCATTC CCTCCCAACT CATGAAGCCA CTGCTAGTGT TTGTAAATCC AAAGAGTGGA
    GGAAACCAGG GAGCTAAAAT CATCCAGACC TTTCTTTGGT ATCTAAACCC TCGGCAGGTG
    TTTGATCTAA GTCAAGGAGG TCCTCAGGAA GGCTTGGAAA TGTATCGCAA GGTTCACAAC
    TTGAGAATTT TGGCCTGTGG GGGGGATGGC ACGGTGGGCT GGATCTTATC TGCACTTGAT
    CAGCTGCAAT TAAACCCTTC TCCTGCTGTG GCCGTACTTC CCCTGGGCAC AGGAAATGAC
    CTTGCTAGGA CGCTAAACTG GGGTGGGGGC TACACAGATG AACCTCTGAG TAAAATCCTG
    TCTCATGTGG AGGATGGGAA TATTGTACAG CTGGATCGGT GGAATCTTGT TGTGAAGCCC
    AACCCAGAGG CAGGACCAGA GGAAAGAGAC GAGCAGGTGA CGGACAAGCT CCCTCTAGAC
    GTCTTCAATA ACTACTTCAG CCTTGGATTT GATGCTCATG TGACTTTAGA GTTCCACGAA
    TCTCGAGAGG CTAATCCTGA GAAATTTAAC AGCCGGTTCA GAAACAAAAT GTTCTATGCA
    GGGACAGCGT TTTCTGATTT CTTGATGGGC AGCTCCAAAG ACCTGGCTAA GCACATCAAA
    GTAGTCTGTG ACGGCACAGA TTTAACCTCA AAGGTCCAGG ATTTGAAGCT GCAATGCCTG
    GTCTTCCTAA ATATCCCCAG GTACTGTGCG GGCACAATGC CATGGGGCAA TCCGAGTGAG
    CATCATGACT TTGAGCCTCA GCGTCATGAT GATGGCTGCA TTGAGGTTAT TGGATTCACA
    ATGACATCAC TGGCCACACT GCAGGTCGGA GGTCACGGTG AGAGGTTAAA TCAGTGTCGC
    GAAGTCACCC TCACCACCTT TAAGTCAATC CCCATGCAGG TGGACGGGGA GCCTTGTAAA
    CTAGCACCAT CTGTTATTCA CATTTCTTTG CGAAACCAGG CCAACATGGT GCAGAAAACC
    AAGAGACGTA CATCTATACC ACTGCTAAAC GACAGTCAGC AACCGTTTCC AGAACGACTA
    CGGATCAGAG TGAGCCGTAT CAGCATGCAT GATTATGAAG CTTTGCACTA CGACAAGGAG
    AAGCTCAAAG AAGCTTCGAT TCCACTGGGT CTCATCGTTG TCCCTGGAGA CAGTGATTTG
    GAAACCTGCC GAGCTCACAT CGAGAGACTA CAGGAGGACT TTGTGTCGTG TAACCCGTCT
    CTGCAGCGGC TGGTGCTCCA GGAAGGTGAC GGGAGCAAAT CAAAGGCTTT GTCGTCTCAG
    AGGCTATCAC CGAAATGGTG CTTTCTCGAC TCTACAACTG CTGATCGATT CTACAGGATT
    GATCGAGCTC AAGAGCATCT CAACTACGTG ACTGAAATTT CTCAGGATGA GCTGTTTGTT
    TTGGACCCAG AGCTGGTGGT CACAGAGACT GTTAGCACAT CACCTGGCAT GCCAGACCTG
    GTGGACTCTG GTGAAGTCCA GTCCGATCCA CACAAATTTG CCTTTCCGTC ATCCTCTTCT
    TCACCACCAA ACACACCTGG ACCTAGGGTT GCAGAGCTGC AAAGGAAGCG TGTTTCCAGT
    GACAGTTCGG TGGCAGAAGC ACTTGCACAC AGTGACTCGC TCAGATCAGC CAAGCCAGCT
    CTCTGCAGAG CTGGGGGTGT TCATCGCTCA AATACAACCG CAGCAGATTT CAAACCTACC
    AGCAGGTTTG AGAAGAGCGC TGTGAACAAT GCCAACTCTG TGTCAGTGGA CAGTCTAATT
    GAATGTGTGA AAAAGAAGGA CCATCAGAAG TTGAAGGAGC TGCATAAGAA AGGGGCCGAC
    CTGTGTGTGC AGGACCCTGC TGGACGGACC CTATTGCACT ATGCTGTGGA AGTGGGCAGC
    AAGGAGATTG TCAAATACAT CATTGATAAT GCACCCACCG ATATCCTGGA TGTAACAGAG
    AAAGAAAATG GAGAGACTGT GTTGCACAAA GCAGCCTCGC TGTGTCATAG GACAATCTGT
    CATTACCTAG TGGAAGCAGG AGCATCTCTT ATGAAGACAG ACTTACAGGG TGACACTCCG
    AAGCATCGTG CCGAGAAGGC GAAAGATGCT GAACTTGCGG CATATCTTGA AAACCGCCAG
    CACTACCAAA TGATCCAGCG TGAGGACCAG GAGACTGCAG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa143499
    Clone ID Related Accession (Same CDS sequence) XM_021477929.1
    Accession Version XM_021477929.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3060bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product diacylglycerol kinase zeta isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTGGGCTC ATAGGAGGAA ATGGGTCTTG CCCCCCACCC CTCCGCATCA ACCGCACCAC 
    AGGAAGGCTA TTGCCAAGTC CGGTCTCCAG CACCTCTCAT GCCAGCCCAG TGCTGCAGTG
    TTAGCCAAAG GGGAAACGGA CAGAGAAATA CGCAACACAG TAGACTGGAG TGAATCAGCT
    GTATACGGAG AGCACATCTG GTTTGAGACG AATGTCTCGG GGGACTTCTG TTACGTTGGA
    GAACAGCACT GTTACGCCAA GTCACTGCAA AAGTCTGTTG CTCGGAAAAA GTGTGCGGCT
    TGCAAGATCG TAGTTCACTC TATCTGCATT GAACAGCTGG AGAAGATAAA CTTCAGATGT
    AAACCGTCTT TCAGGGAGTC AGGATCACGG AACATCCGAG AGCAAACCTG GCGACGCAGA
    GCTTTCAGAG GTCGCTGTGC TTTGGAGCAA ATGAGAGGTG CTGCAGCTAA ATGTACTGCG
    CACACCTCTA ACACACAGCC TGCAATAGTG CGACACCACT GGGTCCATCG GAGAAGACAA
    GAGGGCAAAT GCAAACAGTG TGGAAAGGGG TTTCAGCAGA AATTTGCCTT TCACAGTAAA
    GAGATTGTAG CCATCAGCTG CTCCTGGTGC AAACAAGCAT ATCATAATAA GGTGACATGC
    TTTATGCTGC AGCAGATAGA AGAACCATGT TCATTAGGAG CTCATGCCGC TGTAATAGTA
    CCTCCTACTT GGATCATACG GGTTCGCCGA CCTCAGTCCT CACTAAAGTC AAGCAAAAAG
    AAAAAACGGA CATCTTTCAA ACGTAAATCC AGCAAGAAAG GAGCAGAGGA GGCACGATGG
    AAGCCTTTTA TAGTGAGACC CATTCCCTCC CAACTCATGA AGCCACTGCT AGTGTTTGTA
    AATCCAAAGA GTGGAGGAAA CCAGGGAGCT AAAATCATCC AGACCTTTCT TTGGTATCTA
    AACCCTCGGC AGGTGTTTGA TCTAAGTCAA GGAGGTCCTC AGGAAGGCTT GGAAATGTAT
    CGCAAGGTTC ACAACTTGAG AATTTTGGCC TGTGGGGGGG ATGGCACGGT GGGCTGGATC
    TTATCTGCAC TTGATCAGCT GCAATTAAAC CCTTCTCCTG CTGTGGCCGT ACTTCCCCTG
    GGCACAGGAA ATGACCTTGC TAGGACGCTA AACTGGGGTG GGGGCTACAC AGATGAACCT
    CTGAGTAAAA TCCTGTCTCA TGTGGAGGAT GGGAATATTG TACAGCTGGA TCGGTGGAAT
    CTTGTTGTGA AGCCCAACCC AGAGGCAGGA CCAGAGGAAA GAGACGAGCA GGTGACGGAC
    AAGCTCCCTC TAGACGTCTT CAATAACTAC TTCAGCCTTG GATTTGATGC TCATGTGACT
    TTAGAGTTCC ACGAATCTCG AGAGGCTAAT CCTGAGAAAT TTAACAGCCG GTTCAGAAAC
    AAAATGTTCT ATGCAGGGAC AGCGTTTTCT GATTTCTTGA TGGGCAGCTC CAAAGACCTG
    GCTAAGCACA TCAAAGTAGT CTGTGACGGC ACAGATTTAA CCTCAAAGGT CCAGGATTTG
    AAGCTGCAAT GCCTGGTCTT CCTAAATATC CCCAGGTACT GTGCGGGCAC AATGCCATGG
    GGCAATCCGA GTGAGCATCA TGACTTTGAG CCTCAGCGTC ATGATGATGG CTGCATTGAG
    GTTATTGGAT TCACAATGAC ATCACTGGCC ACACTGCAGG TCGGAGGTCA CGGTGAGAGG
    TTAAATCAGT GTCGCGAAGT CACCCTCACC ACCTTTAAGT CAATCCCCAT GCAGGTGGAC
    GGGGAGCCTT GTAAACTAGC ACCATCTGTT ATTCACATTT CTTTGCGAAA CCAGGCCAAC
    ATGGTGCAGA AAACCAAGAG ACGTACATCT ATACCACTGC TAAACGACAG TCAGCAACCG
    TTTCCAGAAC GACTACGGAT CAGAGTGAGC CGTATCAGCA TGCATGATTA TGAAGCTTTG
    CACTACGACA AGGAGAAGCT CAAAGAAGCT TCGATTCCAC TGGGTCTCAT CGTTGTCCCT
    GGAGACAGTG ATTTGGAAAC CTGCCGAGCT CACATCGAGA GACTACAGGA GGACTTTGTG
    TCGTGTAACC CGTCTCTGCA GCGGCTGGTG CTCCAGGAAG GTGACGGGAG CAAATCAAAG
    GCTTTGTCGT CTCAGAGGCT ATCACCGAAA TGGTGCTTTC TCGACTCTAC AACTGCTGAT
    CGATTCTACA GGATTGATCG AGCTCAAGAG CATCTCAACT ACGTGACTGA AATTTCTCAG
    GATGAGCTGT TTGTTTTGGA CCCAGAGCTG GTGGTCACAG AGACTGTTAG CACATCACCT
    GGCATGCCAG ACCTGGTGGA CTCTGGTGAA GTCCAGTCCG ATCCACACAA ATTTGCCTTT
    CCGTCATCCT CTTCTTCACC ACCAAACACA CCTGGACCTA GGGTTGCAGA GCTGCAAAGG
    AAGCGTGTTT CCAGTGACAG TTCGGTGGCA GAAGCACTTG CACACAGTGA CTCGCTCAGA
    TCAGCCAAGC CAGCTCTCTG CAGAGCTGGG GGTGTTCATC GCTCAAATAC AACCGCAGCA
    GATTTCAAAC CTACCAGCAG GTTTGAGAAG AGCGCTGTGA ACAATGCCAA CTCTGTGTCA
    GTGGACAGTC TAATTGAATG TGTGAAAAAG AAGGACCATC AGAAGTTGAA GGAGCTGCAT
    AAGAAAGGGG CCGACCTGTG TGTGCAGGAC CCTGCTGGAC GGACCCTATT GCACTATGCT
    GTGGAAGTGG GCAGCAAGGA GATTGTCAAA TACATCATTG ATAATGCACC CACCGATATC
    CTGGATGTAA CAGAGAAAGA AAATGGAGAG ACTGTGTTGC ACAAAGCAGC CTCGCTGTGT
    CATAGGACAA TCTGTCATTA CCTAGTGGAA GCAGGAGCAT CTCTTATGAA GACAGACTTA
    CAGGGTGACA CTCCGAAGCA TCGTGCCGAG AAGGCGAAAG ATGCTGAACT TGCGGCATAT
    CTTGAAAACC GCCAGCACTA CCAAATGATC CAGCGTGAGG ACCAGGAGAC TGCAGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333604.1
    CDS151..3210
    Translation

    Target ORF information:

    RefSeq Version XM_021477929.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021477929.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTGGGCTC ATAGGAGGAA ATGGGTCTTG CCCCCCACCC CTCCGCATCA ACCGCACCAC 
    AGGAAGGCTA TTGCCAAGTC CGGTCTCCAG CACCTCTCAT GCCAGCCCAG TGCTGCAGTG
    TTAGCCAAAG GGGAAACGGA CAGAGAAATA CGCAACACAG TAGACTGGAG TGAATCAGCT
    GTATACGGAG AGCACATCTG GTTTGAGACG AATGTCTCGG GGGACTTCTG TTACGTTGGA
    GAACAGCACT GTTACGCCAA GTCACTGCAA AAGTCTGTTG CTCGGAAAAA GTGTGCGGCT
    TGCAAGATCG TAGTTCACTC TATCTGCATT GAACAGCTGG AGAAGATAAA CTTCAGATGT
    AAACCGTCTT TCAGGGAGTC AGGATCACGG AACATCCGAG AGCAAACCTG GCGACGCAGA
    GCTTTCAGAG GTCGCTGTGC TTTGGAGCAA ATGAGAGGTG CTGCAGCTAA ATGTACTGCG
    CACACCTCTA ACACACAGCC TGCAATAGTG CGACACCACT GGGTCCATCG GAGAAGACAA
    GAGGGCAAAT GCAAACAGTG TGGAAAGGGG TTTCAGCAGA AATTTGCCTT TCACAGTAAA
    GAGATTGTAG CCATCAGCTG CTCCTGGTGC AAACAAGCAT ATCATAATAA GGTGACATGC
    TTTATGCTGC AGCAGATAGA AGAACCATGT TCATTAGGAG CTCATGCCGC TGTAATAGTA
    CCTCCTACTT GGATCATACG GGTTCGCCGA CCTCAGTCCT CACTAAAGTC AAGCAAAAAG
    AAAAAACGGA CATCTTTCAA ACGTAAATCC AGCAAGAAAG GAGCAGAGGA GGCACGATGG
    AAGCCTTTTA TAGTGAGACC CATTCCCTCC CAACTCATGA AGCCACTGCT AGTGTTTGTA
    AATCCAAAGA GTGGAGGAAA CCAGGGAGCT AAAATCATCC AGACCTTTCT TTGGTATCTA
    AACCCTCGGC AGGTGTTTGA TCTAAGTCAA GGAGGTCCTC AGGAAGGCTT GGAAATGTAT
    CGCAAGGTTC ACAACTTGAG AATTTTGGCC TGTGGGGGGG ATGGCACGGT GGGCTGGATC
    TTATCTGCAC TTGATCAGCT GCAATTAAAC CCTTCTCCTG CTGTGGCCGT ACTTCCCCTG
    GGCACAGGAA ATGACCTTGC TAGGACGCTA AACTGGGGTG GGGGCTACAC AGATGAACCT
    CTGAGTAAAA TCCTGTCTCA TGTGGAGGAT GGGAATATTG TACAGCTGGA TCGGTGGAAT
    CTTGTTGTGA AGCCCAACCC AGAGGCAGGA CCAGAGGAAA GAGACGAGCA GGTGACGGAC
    AAGCTCCCTC TAGACGTCTT CAATAACTAC TTCAGCCTTG GATTTGATGC TCATGTGACT
    TTAGAGTTCC ACGAATCTCG AGAGGCTAAT CCTGAGAAAT TTAACAGCCG GTTCAGAAAC
    AAAATGTTCT ATGCAGGGAC AGCGTTTTCT GATTTCTTGA TGGGCAGCTC CAAAGACCTG
    GCTAAGCACA TCAAAGTAGT CTGTGACGGC ACAGATTTAA CCTCAAAGGT CCAGGATTTG
    AAGCTGCAAT GCCTGGTCTT CCTAAATATC CCCAGGTACT GTGCGGGCAC AATGCCATGG
    GGCAATCCGA GTGAGCATCA TGACTTTGAG CCTCAGCGTC ATGATGATGG CTGCATTGAG
    GTTATTGGAT TCACAATGAC ATCACTGGCC ACACTGCAGG TCGGAGGTCA CGGTGAGAGG
    TTAAATCAGT GTCGCGAAGT CACCCTCACC ACCTTTAAGT CAATCCCCAT GCAGGTGGAC
    GGGGAGCCTT GTAAACTAGC ACCATCTGTT ATTCACATTT CTTTGCGAAA CCAGGCCAAC
    ATGGTGCAGA AAACCAAGAG ACGTACATCT ATACCACTGC TAAACGACAG TCAGCAACCG
    TTTCCAGAAC GACTACGGAT CAGAGTGAGC CGTATCAGCA TGCATGATTA TGAAGCTTTG
    CACTACGACA AGGAGAAGCT CAAAGAAGCT TCGATTCCAC TGGGTCTCAT CGTTGTCCCT
    GGAGACAGTG ATTTGGAAAC CTGCCGAGCT CACATCGAGA GACTACAGGA GGACTTTGTG
    TCGTGTAACC CGTCTCTGCA GCGGCTGGTG CTCCAGGAAG GTGACGGGAG CAAATCAAAG
    GCTTTGTCGT CTCAGAGGCT ATCACCGAAA TGGTGCTTTC TCGACTCTAC AACTGCTGAT
    CGATTCTACA GGATTGATCG AGCTCAAGAG CATCTCAACT ACGTGACTGA AATTTCTCAG
    GATGAGCTGT TTGTTTTGGA CCCAGAGCTG GTGGTCACAG AGACTGTTAG CACATCACCT
    GGCATGCCAG ACCTGGTGGA CTCTGGTGAA GTCCAGTCCG ATCCACACAA ATTTGCCTTT
    CCGTCATCCT CTTCTTCACC ACCAAACACA CCTGGACCTA GGGTTGCAGA GCTGCAAAGG
    AAGCGTGTTT CCAGTGACAG TTCGGTGGCA GAAGCACTTG CACACAGTGA CTCGCTCAGA
    TCAGCCAAGC CAGCTCTCTG CAGAGCTGGG GGTGTTCATC GCTCAAATAC AACCGCAGCA
    GATTTCAAAC CTACCAGCAG GTTTGAGAAG AGCGCTGTGA ACAATGCCAA CTCTGTGTCA
    GTGGACAGTC TAATTGAATG TGTGAAAAAG AAGGACCATC AGAAGTTGAA GGAGCTGCAT
    AAGAAAGGGG CCGACCTGTG TGTGCAGGAC CCTGCTGGAC GGACCCTATT GCACTATGCT
    GTGGAAGTGG GCAGCAAGGA GATTGTCAAA TACATCATTG ATAATGCACC CACCGATATC
    CTGGATGTAA CAGAGAAAGA AAATGGAGAG ACTGTGTTGC ACAAAGCAGC CTCGCTGTGT
    CATAGGACAA TCTGTCATTA CCTAGTGGAA GCAGGAGCAT CTCTTATGAA GACAGACTTA
    CAGGGTGACA CTCCGAAGCA TCGTGCCGAG AAGGCGAAAG ATGCTGAACT TGCGGCATAT
    CTTGAAAACC GCCAGCACTA CCAAATGATC CAGCGTGAGG ACCAGGAGAC TGCAGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa13277
    Clone ID Related Accession (Same CDS sequence) XM_005169029.3 , XM_005169029.4
    Accession Version XM_005169029.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product diacylglycerol kinase zeta isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169029.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
    CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT
    TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG
    CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA
    GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT
    CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC
    CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC
    CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA
    TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA
    CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC
    CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT
    GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG
    ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC
    AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC
    CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA
    ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG
    ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG
    CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC
    ATTGAACAGC TGGAGAAGTT ATTGGATTGG AATGCTCCAC CTCAGGGGGA GGGGCTTGAT
    GAGATGCCTC TCAAAGAGCC GTCATTCAGA ATAAACTTCA GATGTAAACC GTCTTTCAGG
    GAGTCAGGAT CACGGAACAT CCGAGAGCCT GCAATAGTGC GACACCACTG GGTCCATCGG
    AGAAGACAAG AGGGCAAATG CAAACAGTGT GGAAAGGGGT TTCAGCAGAA ATTTGCCTTT
    CACAGTAAAG AGATTGTAGC CATCAGCTGC TCCTGGTGCA AACAAGCATA TCATAATAAG
    GTGACATGCT TTATGCTGCA GCAGATAGAA GAACCATGTT CATTAGGAGC TCATGCCGCT
    GTAATAGTAC CTCCTACTTG GATCATACGG GTTCGCCGAC CTCAGTCCTC ACTAAAGTCA
    AGCAAAAAGA AAAAACGGAC ATCTTTCAAA CGTAAATCCA GCAAGAAAGG AGCAGAGGAG
    GCACGATGGA AGCCTTTTAT AGTGAGACCC ATTCCCTCCC AACTCATGAA GCCACTGCTA
    GTGTTTGTAA ATCCAAAGAG TGGAGGAAAC CAGGGAGCTA AAATCATCCA GACCTTTCTT
    TGGTATCTAA ACCCTCGGCA GGTGTTTGAT CTAAGTCAAG GAGGTCCTCA GGAAGGCTTG
    GAAATGTATC GCAAGGTTCA CAACTTGAGA ATTTTGGCCT GTGGGGGGGA TGGCACGGTG
    GGCTGGATCT TATCTGCACT TGATCAGCTG CAATTAAACC CTTCTCCTGC TGTGGCCGTA
    CTTCCCCTGG GCACAGGAAA TGACCTTGCT AGGACGCTAA ACTGGGGTGG GGGCTACACA
    GATGAACCTC TGAGTAAAAT CCTGTCTCAT GTGGAGGATG GGAATATTGT ACAGCTGGAT
    CGGTGGAATC TTGTTGTGAA GCCCAACCCA GAGGCAGGAC CAGAGGAAAG AGACGAGCAG
    GTGACGGACA AGCTCCCTCT AGACGTCTTC AATAACTACT TCAGCCTTGG ATTTGATGCT
    CATGTGACTT TAGAGTTCCA CGAATCTCGA GAGGCTAATC CTGAGAAATT TAACAGCCGG
    TTCAGAAACA AAATGTTCTA TGCAGGGACA GCGTTTTCTG ATTTCTTGAT GGGCAGCTCC
    AAAGACCTGG CTAAGCACAT CAAAGTAGTC TGTGACGGCA CAGATTTAAC CTCAAAGGTC
    CAGGATTTGA AGCTGCAATG CCTGGTCTTC CTAAATATCC CCAGGTACTG TGCGGGCACA
    ATGCCATGGG GCAATCCGAG TGAGCATCAT GACTTTGAGC CTCAGCGTCA TGATGATGGC
    TGCATTGAGG TTATTGGATT CACAATGACA TCACTGGCCA CACTGCAGGT CGGAGGTCAC
    GGTGAGAGGT TAAATCAGTG TCGCGAAGTC ACCCTCACCA CCTTTAAGTC AATCCCCATG
    CAGGTGGACG GGGAGCCTTG TAAACTAGCA CCATCTGTTA TTCACATTTC TTTGCGAAAC
    CAGGCCAACA TGGTGCAGAA AACCAAGAGA CGTACATCTA TACCACTGCT AAACGACAGT
    CAGCAACCGT TTCCAGAACG ACTACGGATC AGAGTGAGCC GTATCAGCAT GCATGATTAT
    GAAGCTTTGC ACTACGACAA GGAGAAGCTC AAAGAAGCTT CGATTCCACT GGGTCTCATC
    GTTGTCCCTG GAGACAGTGA TTTGGAAACC TGCCGAGCTC ACATCGAGAG ACTACAGGAG
    GACTTTGTGT CGTGTAACCC GTCTCTGCAG CGGCTGGTGC TCCAGGAAGG TGACGGGAGC
    AAATCAAAGG CTTTGTCGTC TCAGAGGCTA TCACCGAAAT GGTGCTTTCT CGACTCTACA
    ACTGCTGATC GATTCTACAG GATTGATCGA GCTCAAGAGC ATCTCAACTA CGTGACTGAA
    ATTTCTCAGG ATGAGCTGTT TGTTTTGGAC CCAGAGCTGG TGGTCACAGA GACTGTTAGC
    ACATCACCTG GCATGCCAGA CCTGGTGGAC TCTGGTGAAG TCCAGTCCGA TCCACACAAA
    TTTGCCTTTC CGTCATCCTC TTCTTCACCA CCAAACACAC CTGGACCTAG GGTTGCAGAG
    CTGCAAAGGA AGCGTGTTTC CAGTGACAGT TCGGTGGCAG AAGCACTTGC ACACAGTGAC
    TCGCTCAGAT CAGCCAAGCC AGCTCTCTGC AGAGCTGGGG GTGTTCATCG CTCAAATACA
    ACCGCAGCAG ATTTCAAACC TACCAGCAGG TTTGAGAAGA GCGCTGTGAA CAATGCCAAC
    TCTGTGTCAG TGGACAGTCT AATTGAATGT GTGAAAAAGA AGGACCATCA GAAGTTGAAG
    GAGCTGCATA AGAAAGGGGC CGACCTGTGT GTGCAGGACC CTGCTGGACG GACCCTATTG
    CACTATGCTG TGGAAGTGGG CAGCAAGGAG ATTGTCAAAT ACATCATTGA TAATGCACCC
    ACCGATATCC TGGATGTAAC AGAGAAAGAA AATGGAGAGA CTGTGTTGCA CAAAGCAGCC
    TCGCTGTGTC ATAGGACAAT CTGTCATTAC CTAGTGGAAG CAGGAGCATC TCTTATGAAG
    ACAGACTTAC AGGGTGACAC TCCGAAGCAT CGTGCCGAGA AGGCGAAAGA TGCTGAACTT
    GCGGCATATC TTGAAAACCG CCAGCACTAC CAAATGATCC AGCGTGAGGA CCAGGAGACT
    GCAGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005169086.1
    CDS136..3924
    Translation

    Target ORF information:

    RefSeq Version XM_005169029.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005169029.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
    CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT
    TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG
    CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA
    GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT
    CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC
    CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC
    CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA
    TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA
    CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC
    CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT
    GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG
    ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC
    AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC
    CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA
    ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG
    ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG
    CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC
    ATTGAACAGC TGGAGAAGTT ATTGGATTGG AATGCTCCAC CTCAGGGGGA GGGGCTTGAT
    GAGATGCCTC TCAAAGAGCC GTCATTCAGA ATAAACTTCA GATGTAAACC GTCTTTCAGG
    GAGTCAGGAT CACGGAACAT CCGAGAGCCT GCAATAGTGC GACACCACTG GGTCCATCGG
    AGAAGACAAG AGGGCAAATG CAAACAGTGT GGAAAGGGGT TTCAGCAGAA ATTTGCCTTT
    CACAGTAAAG AGATTGTAGC CATCAGCTGC TCCTGGTGCA AACAAGCATA TCATAATAAG
    GTGACATGCT TTATGCTGCA GCAGATAGAA GAACCATGTT CATTAGGAGC TCATGCCGCT
    GTAATAGTAC CTCCTACTTG GATCATACGG GTTCGCCGAC CTCAGTCCTC ACTAAAGTCA
    AGCAAAAAGA AAAAACGGAC ATCTTTCAAA CGTAAATCCA GCAAGAAAGG AGCAGAGGAG
    GCACGATGGA AGCCTTTTAT AGTGAGACCC ATTCCCTCCC AACTCATGAA GCCACTGCTA
    GTGTTTGTAA ATCCAAAGAG TGGAGGAAAC CAGGGAGCTA AAATCATCCA GACCTTTCTT
    TGGTATCTAA ACCCTCGGCA GGTGTTTGAT CTAAGTCAAG GAGGTCCTCA GGAAGGCTTG
    GAAATGTATC GCAAGGTTCA CAACTTGAGA ATTTTGGCCT GTGGGGGGGA TGGCACGGTG
    GGCTGGATCT TATCTGCACT TGATCAGCTG CAATTAAACC CTTCTCCTGC TGTGGCCGTA
    CTTCCCCTGG GCACAGGAAA TGACCTTGCT AGGACGCTAA ACTGGGGTGG GGGCTACACA
    GATGAACCTC TGAGTAAAAT CCTGTCTCAT GTGGAGGATG GGAATATTGT ACAGCTGGAT
    CGGTGGAATC TTGTTGTGAA GCCCAACCCA GAGGCAGGAC CAGAGGAAAG AGACGAGCAG
    GTGACGGACA AGCTCCCTCT AGACGTCTTC AATAACTACT TCAGCCTTGG ATTTGATGCT
    CATGTGACTT TAGAGTTCCA CGAATCTCGA GAGGCTAATC CTGAGAAATT TAACAGCCGG
    TTCAGAAACA AAATGTTCTA TGCAGGGACA GCGTTTTCTG ATTTCTTGAT GGGCAGCTCC
    AAAGACCTGG CTAAGCACAT CAAAGTAGTC TGTGACGGCA CAGATTTAAC CTCAAAGGTC
    CAGGATTTGA AGCTGCAATG CCTGGTCTTC CTAAATATCC CCAGGTACTG TGCGGGCACA
    ATGCCATGGG GCAATCCGAG TGAGCATCAT GACTTTGAGC CTCAGCGTCA TGATGATGGC
    TGCATTGAGG TTATTGGATT CACAATGACA TCACTGGCCA CACTGCAGGT CGGAGGTCAC
    GGTGAGAGGT TAAATCAGTG TCGCGAAGTC ACCCTCACCA CCTTTAAGTC AATCCCCATG
    CAGGTGGACG GGGAGCCTTG TAAACTAGCA CCATCTGTTA TTCACATTTC TTTGCGAAAC
    CAGGCCAACA TGGTGCAGAA AACCAAGAGA CGTACATCTA TACCACTGCT AAACGACAGT
    CAGCAACCGT TTCCAGAACG ACTACGGATC AGAGTGAGCC GTATCAGCAT GCATGATTAT
    GAAGCTTTGC ACTACGACAA GGAGAAGCTC AAAGAAGCTT CGATTCCACT GGGTCTCATC
    GTTGTCCCTG GAGACAGTGA TTTGGAAACC TGCCGAGCTC ACATCGAGAG ACTACAGGAG
    GACTTTGTGT CGTGTAACCC GTCTCTGCAG CGGCTGGTGC TCCAGGAAGG TGACGGGAGC
    AAATCAAAGG CTTTGTCGTC TCAGAGGCTA TCACCGAAAT GGTGCTTTCT CGACTCTACA
    ACTGCTGATC GATTCTACAG GATTGATCGA GCTCAAGAGC ATCTCAACTA CGTGACTGAA
    ATTTCTCAGG ATGAGCTGTT TGTTTTGGAC CCAGAGCTGG TGGTCACAGA GACTGTTAGC
    ACATCACCTG GCATGCCAGA CCTGGTGGAC TCTGGTGAAG TCCAGTCCGA TCCACACAAA
    TTTGCCTTTC CGTCATCCTC TTCTTCACCA CCAAACACAC CTGGACCTAG GGTTGCAGAG
    CTGCAAAGGA AGCGTGTTTC CAGTGACAGT TCGGTGGCAG AAGCACTTGC ACACAGTGAC
    TCGCTCAGAT CAGCCAAGCC AGCTCTCTGC AGAGCTGGGG GTGTTCATCG CTCAAATACA
    ACCGCAGCAG ATTTCAAACC TACCAGCAGG TTTGAGAAGA GCGCTGTGAA CAATGCCAAC
    TCTGTGTCAG TGGACAGTCT AATTGAATGT GTGAAAAAGA AGGACCATCA GAAGTTGAAG
    GAGCTGCATA AGAAAGGGGC CGACCTGTGT GTGCAGGACC CTGCTGGACG GACCCTATTG
    CACTATGCTG TGGAAGTGGG CAGCAAGGAG ATTGTCAAAT ACATCATTGA TAATGCACCC
    ACCGATATCC TGGATGTAAC AGAGAAAGAA AATGGAGAGA CTGTGTTGCA CAAAGCAGCC
    TCGCTGTGTC ATAGGACAAT CTGTCATTAC CTAGTGGAAG CAGGAGCATC TCTTATGAAG
    ACAGACTTAC AGGGTGACAC TCCGAAGCAT CGTGCCGAGA AGGCGAAAGA TGCTGAACTT
    GCGGCATATC TTGAAAACCG CCAGCACTAC CAAATGATCC AGCGTGAGGA CCAGGAGACT
    GCAGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa13277
    Clone ID Related Accession (Same CDS sequence) XM_005169029.3 , XM_005169029.4
    Accession Version XM_005169029.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product diacylglycerol kinase zeta isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005169029.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
    CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT
    TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG
    CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA
    GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT
    CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC
    CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC
    CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA
    TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA
    CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC
    CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT
    GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG
    ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC
    AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC
    CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA
    ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG
    ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG
    CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC
    ATTGAACAGC TGGAGAAGTT ATTGGATTGG AATGCTCCAC CTCAGGGGGA GGGGCTTGAT
    GAGATGCCTC TCAAAGAGCC GTCATTCAGA ATAAACTTCA GATGTAAACC GTCTTTCAGG
    GAGTCAGGAT CACGGAACAT CCGAGAGCCT GCAATAGTGC GACACCACTG GGTCCATCGG
    AGAAGACAAG AGGGCAAATG CAAACAGTGT GGAAAGGGGT TTCAGCAGAA ATTTGCCTTT
    CACAGTAAAG AGATTGTAGC CATCAGCTGC TCCTGGTGCA AACAAGCATA TCATAATAAG
    GTGACATGCT TTATGCTGCA GCAGATAGAA GAACCATGTT CATTAGGAGC TCATGCCGCT
    GTAATAGTAC CTCCTACTTG GATCATACGG GTTCGCCGAC CTCAGTCCTC ACTAAAGTCA
    AGCAAAAAGA AAAAACGGAC ATCTTTCAAA CGTAAATCCA GCAAGAAAGG AGCAGAGGAG
    GCACGATGGA AGCCTTTTAT AGTGAGACCC ATTCCCTCCC AACTCATGAA GCCACTGCTA
    GTGTTTGTAA ATCCAAAGAG TGGAGGAAAC CAGGGAGCTA AAATCATCCA GACCTTTCTT
    TGGTATCTAA ACCCTCGGCA GGTGTTTGAT CTAAGTCAAG GAGGTCCTCA GGAAGGCTTG
    GAAATGTATC GCAAGGTTCA CAACTTGAGA ATTTTGGCCT GTGGGGGGGA TGGCACGGTG
    GGCTGGATCT TATCTGCACT TGATCAGCTG CAATTAAACC CTTCTCCTGC TGTGGCCGTA
    CTTCCCCTGG GCACAGGAAA TGACCTTGCT AGGACGCTAA ACTGGGGTGG GGGCTACACA
    GATGAACCTC TGAGTAAAAT CCTGTCTCAT GTGGAGGATG GGAATATTGT ACAGCTGGAT
    CGGTGGAATC TTGTTGTGAA GCCCAACCCA GAGGCAGGAC CAGAGGAAAG AGACGAGCAG
    GTGACGGACA AGCTCCCTCT AGACGTCTTC AATAACTACT TCAGCCTTGG ATTTGATGCT
    CATGTGACTT TAGAGTTCCA CGAATCTCGA GAGGCTAATC CTGAGAAATT TAACAGCCGG
    TTCAGAAACA AAATGTTCTA TGCAGGGACA GCGTTTTCTG ATTTCTTGAT GGGCAGCTCC
    AAAGACCTGG CTAAGCACAT CAAAGTAGTC TGTGACGGCA CAGATTTAAC CTCAAAGGTC
    CAGGATTTGA AGCTGCAATG CCTGGTCTTC CTAAATATCC CCAGGTACTG TGCGGGCACA
    ATGCCATGGG GCAATCCGAG TGAGCATCAT GACTTTGAGC CTCAGCGTCA TGATGATGGC
    TGCATTGAGG TTATTGGATT CACAATGACA TCACTGGCCA CACTGCAGGT CGGAGGTCAC
    GGTGAGAGGT TAAATCAGTG TCGCGAAGTC ACCCTCACCA CCTTTAAGTC AATCCCCATG
    CAGGTGGACG GGGAGCCTTG TAAACTAGCA CCATCTGTTA TTCACATTTC TTTGCGAAAC
    CAGGCCAACA TGGTGCAGAA AACCAAGAGA CGTACATCTA TACCACTGCT AAACGACAGT
    CAGCAACCGT TTCCAGAACG ACTACGGATC AGAGTGAGCC GTATCAGCAT GCATGATTAT
    GAAGCTTTGC ACTACGACAA GGAGAAGCTC AAAGAAGCTT CGATTCCACT GGGTCTCATC
    GTTGTCCCTG GAGACAGTGA TTTGGAAACC TGCCGAGCTC ACATCGAGAG ACTACAGGAG
    GACTTTGTGT CGTGTAACCC GTCTCTGCAG CGGCTGGTGC TCCAGGAAGG TGACGGGAGC
    AAATCAAAGG CTTTGTCGTC TCAGAGGCTA TCACCGAAAT GGTGCTTTCT CGACTCTACA
    ACTGCTGATC GATTCTACAG GATTGATCGA GCTCAAGAGC ATCTCAACTA CGTGACTGAA
    ATTTCTCAGG ATGAGCTGTT TGTTTTGGAC CCAGAGCTGG TGGTCACAGA GACTGTTAGC
    ACATCACCTG GCATGCCAGA CCTGGTGGAC TCTGGTGAAG TCCAGTCCGA TCCACACAAA
    TTTGCCTTTC CGTCATCCTC TTCTTCACCA CCAAACACAC CTGGACCTAG GGTTGCAGAG
    CTGCAAAGGA AGCGTGTTTC CAGTGACAGT TCGGTGGCAG AAGCACTTGC ACACAGTGAC
    TCGCTCAGAT CAGCCAAGCC AGCTCTCTGC AGAGCTGGGG GTGTTCATCG CTCAAATACA
    ACCGCAGCAG ATTTCAAACC TACCAGCAGG TTTGAGAAGA GCGCTGTGAA CAATGCCAAC
    TCTGTGTCAG TGGACAGTCT AATTGAATGT GTGAAAAAGA AGGACCATCA GAAGTTGAAG
    GAGCTGCATA AGAAAGGGGC CGACCTGTGT GTGCAGGACC CTGCTGGACG GACCCTATTG
    CACTATGCTG TGGAAGTGGG CAGCAAGGAG ATTGTCAAAT ACATCATTGA TAATGCACCC
    ACCGATATCC TGGATGTAAC AGAGAAAGAA AATGGAGAGA CTGTGTTGCA CAAAGCAGCC
    TCGCTGTGTC ATAGGACAAT CTGTCATTAC CTAGTGGAAG CAGGAGCATC TCTTATGAAG
    ACAGACTTAC AGGGTGACAC TCCGAAGCAT CGTGCCGAGA AGGCGAAAGA TGCTGAACTT
    GCGGCATATC TTGAAAACCG CCAGCACTAC CAAATGATCC AGCGTGAGGA CCAGGAGACT
    GCAGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005169086.1
    CDS171..3959
    Translation

    Target ORF information:

    RefSeq Version XM_005169029.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005169029.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
    CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT
    TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG
    CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA
    GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT
    CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC
    CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC
    CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA
    TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA
    CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC
    CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT
    GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG
    ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC
    AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC
    CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA
    ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG
    ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG
    CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC
    ATTGAACAGC TGGAGAAGTT ATTGGATTGG AATGCTCCAC CTCAGGGGGA GGGGCTTGAT
    GAGATGCCTC TCAAAGAGCC GTCATTCAGA ATAAACTTCA GATGTAAACC GTCTTTCAGG
    GAGTCAGGAT CACGGAACAT CCGAGAGCCT GCAATAGTGC GACACCACTG GGTCCATCGG
    AGAAGACAAG AGGGCAAATG CAAACAGTGT GGAAAGGGGT TTCAGCAGAA ATTTGCCTTT
    CACAGTAAAG AGATTGTAGC CATCAGCTGC TCCTGGTGCA AACAAGCATA TCATAATAAG
    GTGACATGCT TTATGCTGCA GCAGATAGAA GAACCATGTT CATTAGGAGC TCATGCCGCT
    GTAATAGTAC CTCCTACTTG GATCATACGG GTTCGCCGAC CTCAGTCCTC ACTAAAGTCA
    AGCAAAAAGA AAAAACGGAC ATCTTTCAAA CGTAAATCCA GCAAGAAAGG AGCAGAGGAG
    GCACGATGGA AGCCTTTTAT AGTGAGACCC ATTCCCTCCC AACTCATGAA GCCACTGCTA
    GTGTTTGTAA ATCCAAAGAG TGGAGGAAAC CAGGGAGCTA AAATCATCCA GACCTTTCTT
    TGGTATCTAA ACCCTCGGCA GGTGTTTGAT CTAAGTCAAG GAGGTCCTCA GGAAGGCTTG
    GAAATGTATC GCAAGGTTCA CAACTTGAGA ATTTTGGCCT GTGGGGGGGA TGGCACGGTG
    GGCTGGATCT TATCTGCACT TGATCAGCTG CAATTAAACC CTTCTCCTGC TGTGGCCGTA
    CTTCCCCTGG GCACAGGAAA TGACCTTGCT AGGACGCTAA ACTGGGGTGG GGGCTACACA
    GATGAACCTC TGAGTAAAAT CCTGTCTCAT GTGGAGGATG GGAATATTGT ACAGCTGGAT
    CGGTGGAATC TTGTTGTGAA GCCCAACCCA GAGGCAGGAC CAGAGGAAAG AGACGAGCAG
    GTGACGGACA AGCTCCCTCT AGACGTCTTC AATAACTACT TCAGCCTTGG ATTTGATGCT
    CATGTGACTT TAGAGTTCCA CGAATCTCGA GAGGCTAATC CTGAGAAATT TAACAGCCGG
    TTCAGAAACA AAATGTTCTA TGCAGGGACA GCGTTTTCTG ATTTCTTGAT GGGCAGCTCC
    AAAGACCTGG CTAAGCACAT CAAAGTAGTC TGTGACGGCA CAGATTTAAC CTCAAAGGTC
    CAGGATTTGA AGCTGCAATG CCTGGTCTTC CTAAATATCC CCAGGTACTG TGCGGGCACA
    ATGCCATGGG GCAATCCGAG TGAGCATCAT GACTTTGAGC CTCAGCGTCA TGATGATGGC
    TGCATTGAGG TTATTGGATT CACAATGACA TCACTGGCCA CACTGCAGGT CGGAGGTCAC
    GGTGAGAGGT TAAATCAGTG TCGCGAAGTC ACCCTCACCA CCTTTAAGTC AATCCCCATG
    CAGGTGGACG GGGAGCCTTG TAAACTAGCA CCATCTGTTA TTCACATTTC TTTGCGAAAC
    CAGGCCAACA TGGTGCAGAA AACCAAGAGA CGTACATCTA TACCACTGCT AAACGACAGT
    CAGCAACCGT TTCCAGAACG ACTACGGATC AGAGTGAGCC GTATCAGCAT GCATGATTAT
    GAAGCTTTGC ACTACGACAA GGAGAAGCTC AAAGAAGCTT CGATTCCACT GGGTCTCATC
    GTTGTCCCTG GAGACAGTGA TTTGGAAACC TGCCGAGCTC ACATCGAGAG ACTACAGGAG
    GACTTTGTGT CGTGTAACCC GTCTCTGCAG CGGCTGGTGC TCCAGGAAGG TGACGGGAGC
    AAATCAAAGG CTTTGTCGTC TCAGAGGCTA TCACCGAAAT GGTGCTTTCT CGACTCTACA
    ACTGCTGATC GATTCTACAG GATTGATCGA GCTCAAGAGC ATCTCAACTA CGTGACTGAA
    ATTTCTCAGG ATGAGCTGTT TGTTTTGGAC CCAGAGCTGG TGGTCACAGA GACTGTTAGC
    ACATCACCTG GCATGCCAGA CCTGGTGGAC TCTGGTGAAG TCCAGTCCGA TCCACACAAA
    TTTGCCTTTC CGTCATCCTC TTCTTCACCA CCAAACACAC CTGGACCTAG GGTTGCAGAG
    CTGCAAAGGA AGCGTGTTTC CAGTGACAGT TCGGTGGCAG AAGCACTTGC ACACAGTGAC
    TCGCTCAGAT CAGCCAAGCC AGCTCTCTGC AGAGCTGGGG GTGTTCATCG CTCAAATACA
    ACCGCAGCAG ATTTCAAACC TACCAGCAGG TTTGAGAAGA GCGCTGTGAA CAATGCCAAC
    TCTGTGTCAG TGGACAGTCT AATTGAATGT GTGAAAAAGA AGGACCATCA GAAGTTGAAG
    GAGCTGCATA AGAAAGGGGC CGACCTGTGT GTGCAGGACC CTGCTGGACG GACCCTATTG
    CACTATGCTG TGGAAGTGGG CAGCAAGGAG ATTGTCAAAT ACATCATTGA TAATGCACCC
    ACCGATATCC TGGATGTAAC AGAGAAAGAA AATGGAGAGA CTGTGTTGCA CAAAGCAGCC
    TCGCTGTGTC ATAGGACAAT CTGTCATTAC CTAGTGGAAG CAGGAGCATC TCTTATGAAG
    ACAGACTTAC AGGGTGACAC TCCGAAGCAT CGTGCCGAGA AGGCGAAAGA TGCTGAACTT
    GCGGCATATC TTGAAAACCG CCAGCACTAC CAAATGATCC AGCGTGAGGA CCAGGAGACT
    GCAGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa13278
    Clone ID Related Accession (Same CDS sequence) XM_005169030.3
    Accession Version XM_005169030.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3744bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product diacylglycerol kinase zeta isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169030.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
    CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT
    TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG
    CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA
    GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT
    CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC
    CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC
    CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA
    TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA
    CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC
    CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT
    GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG
    ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC
    AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC
    CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA
    ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG
    ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG
    CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC
    ATTGAACAGC TGGAGAAGTT ATTGGATTGG AATGCTCCAC CTCAGGGGGA GGGGCTTGAT
    GAGATGCCTC TCAAAGAGCC GTCATTCAGA ATAAACTTCA GATGTAAACC GTCTTTCAGG
    GAGTCAGGAT CACGGAACAT CCGAGAGCCT GCAATAGTGC GACACCACTG GGTCCATCGG
    AGAAGACAAG AGGGCAAATG CAAACAGTGT GGAAAGGGGT TTCAGCAGAA ATTTGCCTTT
    CACAGTAAAG AGATTGTAGC CATCAGCTGC TCCTGGTGCA AACAAGCATA TCATAATAAG
    GTGACATGCT TTATGCTGCA GCAGATAGAA GAACCATGTT CATTAGGAGC TCATGCCGCT
    GTAATAGTAC CTCCTACTTG GATCATACGG GTTCGCCGAC CTCAGTCCTC ACTAAAGTCA
    AGCAAAAAGA AAAAACGGAC ATCTTTCAAA CGTAAATCCA GCAAGAAAGG AGCAGAGGAG
    GCACGATGGA AGCCTTTTAT AGTGAGACCC ATTCCCTCCC AACTCATGAA GCCACTGCTA
    GTGTTTGTAA ATCCAAAGAG TGGAGGAAAC CAGGGAGCTA AAATCATCCA GACCTTTCTT
    TGGTATCTAA ACCCTCGGCA GGTGTTTGAT CTAAGTCAAG GAGGTCCTCA GGAAGGCTTG
    GAAATGTATC GCAAGGTTCA CAACTTGAGA ATTTTGGCCT GTGGGGGGGA TGGCACGGTG
    GGCTGGATCT TATCTGCACT TGATCAGCTG CAATTAAACC CTTCTCCTGC TGTGGCCGTA
    CTTCCCCTGG GCACAGGAAA TGACCTTGCT AGGACGCTAA ACTGGGGTGG GGGCTACACA
    GATGAACCTC TGAGTAAAAT CCTGTCTCAT GTGGAGGATG GGAATATTGT ACAGCTGGAT
    CGGTGGAATC TTGTTGTGAA GCCCAACCCA GAGGCAGGAC CAGAGGAAAG AGACGAGCAG
    GTGACGGACA AGCTCCCTCT AGACGTCTTC AATAACTACT TCAGCCTTGG ATTTGATGCT
    CATGTGACTT TAGAGTTCCA CGAATCTCGA GAGGCTAATC CTGAGAAATT TAACAGCCGG
    TTCAGAAACA AAATGTTCTA TGCAGGGACA GCGTTTTCTG ATTTCTTGAT GGGCAGCTCC
    AAAGACCTGG CTAAGCACAT CAAAGTAGTC TGTGACGGCA CAGATTTAAC CTCAAAGGTC
    CAGGATTTGA AGCTGCAATG CCTGGTCTTC CTAAATATCC CCAGGTACTG TGCGGGCACA
    ATGCCATGGG GCAATCCGAG TGAGCATCAT GACTTTGAGC CTCAGCGTCA TGATGATGGC
    TGCATTGAGG TTATTGGATT CACAATGACA TCACTGGCCA CACTGCAGGT CGGAGGTCAC
    GGTGAGAGGT TAAATCAGTG TCGCGAAGTC ACCCTCACCA CCTTTAAGTC AATCCCCATG
    CAGGTGGACG GGGAGCCTTG TAAACTAGCA CCATCTGTTA TTCACATTTC TTTGCGAAAC
    CAGGCCAACA TGGTGCAGAA AACCAAGAGA CGTACATCTA TACCACTGCT AAACGACAGT
    CAGCAACCGT TTCCAGAACG ACTACGGATC AGAGTGAGCC GTATCAGCAT GCATGATTAT
    GAAGCTTTGC ACTACGACAA GGAGAAGCTC AAAGAAGCTT CGATTCCACT GGGTCTCATC
    GTTGTCCCTG GAGACAGTGA TTTGGAAACC TGCCGAGCTC ACATCGAGAG ACTACAGGAG
    GAAGGTGACG GGAGCAAATC AAAGGCTTTG TCGTCTCAGA GGCTATCACC GAAATGGTGC
    TTTCTCGACT CTACAACTGC TGATCGATTC TACAGGATTG ATCGAGCTCA AGAGCATCTC
    AACTACGTGA CTGAAATTTC TCAGGATGAG CTGTTTGTTT TGGACCCAGA GCTGGTGGTC
    ACAGAGACTG TTAGCACATC ACCTGGCATG CCAGACCTGG TGGACTCTGG TGAAGTCCAG
    TCCGATCCAC ACAAATTTGC CTTTCCGTCA TCCTCTTCTT CACCACCAAA CACACCTGGA
    CCTAGGGTTG CAGAGCTGCA AAGGAAGCGT GTTTCCAGTG ACAGTTCGGT GGCAGAAGCA
    CTTGCACACA GTGACTCGCT CAGATCAGCC AAGCCAGCTC TCTGCAGAGC TGGGGGTGTT
    CATCGCTCAA ATACAACCGC AGCAGATTTC AAACCTACCA GCAGGTTTGA GAAGAGCGCT
    GTGAACAATG CCAACTCTGT GTCAGTGGAC AGTCTAATTG AATGTGTGAA AAAGAAGGAC
    CATCAGAAGT TGAAGGAGCT GCATAAGAAA GGGGCCGACC TGTGTGTGCA GGACCCTGCT
    GGACGGACCC TATTGCACTA TGCTGTGGAA GTGGGCAGCA AGGAGATTGT CAAATACATC
    ATTGATAATG CACCCACCGA TATCCTGGAT GTAACAGAGA AAGAAAATGG AGAGACTGTG
    TTGCACAAAG CAGCCTCGCT GTGTCATAGG ACAATCTGTC ATTACCTAGT GGAAGCAGGA
    GCATCTCTTA TGAAGACAGA CTTACAGGGT GACACTCCGA AGCATCGTGC CGAGAAGGCG
    AAAGATGCTG AACTTGCGGC ATATCTTGAA AACCGCCAGC ACTACCAAAT GATCCAGCGT
    GAGGACCAGG AGACTGCAGT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005169087.1
    CDS136..3879
    Translation

    Target ORF information:

    RefSeq Version XM_005169030.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005169030.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
    CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT
    TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG
    CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA
    GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT
    CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC
    CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC
    CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA
    TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA
    CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC
    CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT
    GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG
    ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC
    AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC
    CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA
    ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG
    ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG
    CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC
    ATTGAACAGC TGGAGAAGTT ATTGGATTGG AATGCTCCAC CTCAGGGGGA GGGGCTTGAT
    GAGATGCCTC TCAAAGAGCC GTCATTCAGA ATAAACTTCA GATGTAAACC GTCTTTCAGG
    GAGTCAGGAT CACGGAACAT CCGAGAGCCT GCAATAGTGC GACACCACTG GGTCCATCGG
    AGAAGACAAG AGGGCAAATG CAAACAGTGT GGAAAGGGGT TTCAGCAGAA ATTTGCCTTT
    CACAGTAAAG AGATTGTAGC CATCAGCTGC TCCTGGTGCA AACAAGCATA TCATAATAAG
    GTGACATGCT TTATGCTGCA GCAGATAGAA GAACCATGTT CATTAGGAGC TCATGCCGCT
    GTAATAGTAC CTCCTACTTG GATCATACGG GTTCGCCGAC CTCAGTCCTC ACTAAAGTCA
    AGCAAAAAGA AAAAACGGAC ATCTTTCAAA CGTAAATCCA GCAAGAAAGG AGCAGAGGAG
    GCACGATGGA AGCCTTTTAT AGTGAGACCC ATTCCCTCCC AACTCATGAA GCCACTGCTA
    GTGTTTGTAA ATCCAAAGAG TGGAGGAAAC CAGGGAGCTA AAATCATCCA GACCTTTCTT
    TGGTATCTAA ACCCTCGGCA GGTGTTTGAT CTAAGTCAAG GAGGTCCTCA GGAAGGCTTG
    GAAATGTATC GCAAGGTTCA CAACTTGAGA ATTTTGGCCT GTGGGGGGGA TGGCACGGTG
    GGCTGGATCT TATCTGCACT TGATCAGCTG CAATTAAACC CTTCTCCTGC TGTGGCCGTA
    CTTCCCCTGG GCACAGGAAA TGACCTTGCT AGGACGCTAA ACTGGGGTGG GGGCTACACA
    GATGAACCTC TGAGTAAAAT CCTGTCTCAT GTGGAGGATG GGAATATTGT ACAGCTGGAT
    CGGTGGAATC TTGTTGTGAA GCCCAACCCA GAGGCAGGAC CAGAGGAAAG AGACGAGCAG
    GTGACGGACA AGCTCCCTCT AGACGTCTTC AATAACTACT TCAGCCTTGG ATTTGATGCT
    CATGTGACTT TAGAGTTCCA CGAATCTCGA GAGGCTAATC CTGAGAAATT TAACAGCCGG
    TTCAGAAACA AAATGTTCTA TGCAGGGACA GCGTTTTCTG ATTTCTTGAT GGGCAGCTCC
    AAAGACCTGG CTAAGCACAT CAAAGTAGTC TGTGACGGCA CAGATTTAAC CTCAAAGGTC
    CAGGATTTGA AGCTGCAATG CCTGGTCTTC CTAAATATCC CCAGGTACTG TGCGGGCACA
    ATGCCATGGG GCAATCCGAG TGAGCATCAT GACTTTGAGC CTCAGCGTCA TGATGATGGC
    TGCATTGAGG TTATTGGATT CACAATGACA TCACTGGCCA CACTGCAGGT CGGAGGTCAC
    GGTGAGAGGT TAAATCAGTG TCGCGAAGTC ACCCTCACCA CCTTTAAGTC AATCCCCATG
    CAGGTGGACG GGGAGCCTTG TAAACTAGCA CCATCTGTTA TTCACATTTC TTTGCGAAAC
    CAGGCCAACA TGGTGCAGAA AACCAAGAGA CGTACATCTA TACCACTGCT AAACGACAGT
    CAGCAACCGT TTCCAGAACG ACTACGGATC AGAGTGAGCC GTATCAGCAT GCATGATTAT
    GAAGCTTTGC ACTACGACAA GGAGAAGCTC AAAGAAGCTT CGATTCCACT GGGTCTCATC
    GTTGTCCCTG GAGACAGTGA TTTGGAAACC TGCCGAGCTC ACATCGAGAG ACTACAGGAG
    GAAGGTGACG GGAGCAAATC AAAGGCTTTG TCGTCTCAGA GGCTATCACC GAAATGGTGC
    TTTCTCGACT CTACAACTGC TGATCGATTC TACAGGATTG ATCGAGCTCA AGAGCATCTC
    AACTACGTGA CTGAAATTTC TCAGGATGAG CTGTTTGTTT TGGACCCAGA GCTGGTGGTC
    ACAGAGACTG TTAGCACATC ACCTGGCATG CCAGACCTGG TGGACTCTGG TGAAGTCCAG
    TCCGATCCAC ACAAATTTGC CTTTCCGTCA TCCTCTTCTT CACCACCAAA CACACCTGGA
    CCTAGGGTTG CAGAGCTGCA AAGGAAGCGT GTTTCCAGTG ACAGTTCGGT GGCAGAAGCA
    CTTGCACACA GTGACTCGCT CAGATCAGCC AAGCCAGCTC TCTGCAGAGC TGGGGGTGTT
    CATCGCTCAA ATACAACCGC AGCAGATTTC AAACCTACCA GCAGGTTTGA GAAGAGCGCT
    GTGAACAATG CCAACTCTGT GTCAGTGGAC AGTCTAATTG AATGTGTGAA AAAGAAGGAC
    CATCAGAAGT TGAAGGAGCT GCATAAGAAA GGGGCCGACC TGTGTGTGCA GGACCCTGCT
    GGACGGACCC TATTGCACTA TGCTGTGGAA GTGGGCAGCA AGGAGATTGT CAAATACATC
    ATTGATAATG CACCCACCGA TATCCTGGAT GTAACAGAGA AAGAAAATGG AGAGACTGTG
    TTGCACAAAG CAGCCTCGCT GTGTCATAGG ACAATCTGTC ATTACCTAGT GGAAGCAGGA
    GCATCTCTTA TGAAGACAGA CTTACAGGGT GACACTCCGA AGCATCGTGC CGAGAAGGCG
    AAAGATGCTG AACTTGCGGC ATATCTTGAA AACCGCCAGC ACTACCAAAT GATCCAGCGT
    GAGGACCAGG AGACTGCAGT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa13279
    Clone ID Related Accession (Same CDS sequence) XM_005169031.3
    Accession Version XM_005169031.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3741bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product diacylglycerol kinase zeta isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169031.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
    CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT
    TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG
    CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA
    GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT
    CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC
    CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC
    CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA
    TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA
    CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC
    CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT
    GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG
    ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC
    AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC
    CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA
    ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG
    ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG
    CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC
    ATTGAACAGC TGGAGAAGTT ATTGGATTGG AATGCTCCAC CTCAGGGGGA GGGGCTTGAT
    GAGATGCCTC TCAAAGAGCC GTCATTCAGA ATAAACTTCA GATGTAAACC GTCTTTCAGG
    GAGTCAGGAT CACGGAACAT CCGAGAGCCT GCAATAGTGC GACACCACTG GGTCCATCGG
    AGAAGACAAG AGGGCAAATG CAAACAGTGT GGAAAGGGGT TTCAGCAGAA ATTTGCCTTT
    CACAGTAAAG AGATTGTAGC CATCAGCTGC TCCTGGTGCA AACAAGCATA TCATAATAAG
    GTGACATGCT TTATGCTGCA GCAGATAGAA GAACCATGTT CATTAGGAGC TCATGCCGCT
    GTAATAGTAC CTCCTACTTG GATCATACGG GTTCGCCGAC CTCAGTCCTC ACTAAAGTCA
    AGCAAAAAGA AAAAACGGAC ATCTTTCAAA CGTAAATCCA GCAAGAAAGG AGCAGAGGAG
    GCACGATGGA AGCCTTTTAT AGTGAGACCC ATTCCCTCCC AACTCATGAA GCCACTGCTA
    GTGTTTGTAA ATCCAAAGAG TGGAGGAAAC CAGGGAGCTA AAATCATCCA GACCTTTCTT
    TGGTATCTAA ACCCTCGGCA GGTGTTTGAT CTAAGTCAAG GAGGTCCTCA GGAAGGCTTG
    GAAATGTATC GCAAGGTTCA CAACTTGAGA ATTTTGGCCT GTGGGGGGGA TGGCACGGTG
    GGCTGGATCT TATCTGCACT TGATCAGCTG CAATTAAACC CTTCTCCTGC TGTGGCCGTA
    CTTCCCCTGG GCACAGGAAA TGACCTTGCT AGGACGCTAA ACTGGGGTGG GGGCTACACA
    GATGAACCTC TGAGTAAAAT CCTGTCTCAT GTGGAGGATG GGAATATTGT ACAGCTGGAT
    CGGTGGAATC TTGTTGTGAA GCCCAACCCA GAGGCAGGAC CAGAGGAAAG AGACGAGCAG
    GTGACGGACA AGCTCCCTCT AGACGTCTTC AATAACTACT TCAGCCTTGG ATTTGATGCT
    CATGTGACTT TAGAGTTCCA CGAATCTCGA GAGGCTAATC CTGAGAAATT TAACAGCCGG
    TTCAGAAACA AAATGTTCTA TGCAGGGACA GCGTTTTCTG ATTTCTTGAT GGGCAGCTCC
    AAAGACCTGG CTAAGCACAT CAAAGTAGTC TGTGACGGCA CAGATTTAAC CTCAAAGGTC
    CAGGATTTGA AGCTGCAATG CCTGGTCTTC CTAAATATCC CCAGGTACTG TGCGGGCACA
    ATGCCATGGG GCAATCCGAG TGAGCATCAT GACTTTGAGC CTCAGCGTCA TGATGATGGC
    TGCATTGAGG TTATTGGATT CACAATGACA TCACTGGCCA CACTGCAGGT CGGAGGTCAC
    GGTGAGAGGT TAAATCAGTG TCGCGAAGTC ACCCTCACCA CCTTTAAGTC AATCCCCATG
    CAGGTGGACG GGGAGCCTTG TAAACTAGCA CCATCTGTTA TTCACATTTC TTTGCGAAAC
    CAGGCCAACA TGGTGCAGAA AACCAAGAGA CGTACATCTA TACCACTGCT AAACGATCAG
    CAACCGTTTC CAGAACGACT ACGGATCAGA GTGAGCCGTA TCAGCATGCA TGATTATGAA
    GCTTTGCACT ACGACAAGGA GAAGCTCAAA GAAGCTTCGA TTCCACTGGG TCTCATCGTT
    GTCCCTGGAG ACAGTGATTT GGAAACCTGC CGAGCTCACA TCGAGAGACT ACAGGAGGAA
    GGTGACGGGA GCAAATCAAA GGCTTTGTCG TCTCAGAGGC TATCACCGAA ATGGTGCTTT
    CTCGACTCTA CAACTGCTGA TCGATTCTAC AGGATTGATC GAGCTCAAGA GCATCTCAAC
    TACGTGACTG AAATTTCTCA GGATGAGCTG TTTGTTTTGG ACCCAGAGCT GGTGGTCACA
    GAGACTGTTA GCACATCACC TGGCATGCCA GACCTGGTGG ACTCTGGTGA AGTCCAGTCC
    GATCCACACA AATTTGCCTT TCCGTCATCC TCTTCTTCAC CACCAAACAC ACCTGGACCT
    AGGGTTGCAG AGCTGCAAAG GAAGCGTGTT TCCAGTGACA GTTCGGTGGC AGAAGCACTT
    GCACACAGTG ACTCGCTCAG ATCAGCCAAG CCAGCTCTCT GCAGAGCTGG GGGTGTTCAT
    CGCTCAAATA CAACCGCAGC AGATTTCAAA CCTACCAGCA GGTTTGAGAA GAGCGCTGTG
    AACAATGCCA ACTCTGTGTC AGTGGACAGT CTAATTGAAT GTGTGAAAAA GAAGGACCAT
    CAGAAGTTGA AGGAGCTGCA TAAGAAAGGG GCCGACCTGT GTGTGCAGGA CCCTGCTGGA
    CGGACCCTAT TGCACTATGC TGTGGAAGTG GGCAGCAAGG AGATTGTCAA ATACATCATT
    GATAATGCAC CCACCGATAT CCTGGATGTA ACAGAGAAAG AAAATGGAGA GACTGTGTTG
    CACAAAGCAG CCTCGCTGTG TCATAGGACA ATCTGTCATT ACCTAGTGGA AGCAGGAGCA
    TCTCTTATGA AGACAGACTT ACAGGGTGAC ACTCCGAAGC ATCGTGCCGA GAAGGCGAAA
    GATGCTGAAC TTGCGGCATA TCTTGAAAAC CGCCAGCACT ACCAAATGAT CCAGCGTGAG
    GACCAGGAGA CTGCAGTTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005169088.1
    CDS136..3876
    Translation

    Target ORF information:

    RefSeq Version XM_005169031.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005169031.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
    CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT
    TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG
    CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA
    GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT
    CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC
    CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC
    CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA
    TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA
    CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC
    CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT
    GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG
    ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC
    AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC
    CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA
    ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG
    ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG
    CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC
    ATTGAACAGC TGGAGAAGTT ATTGGATTGG AATGCTCCAC CTCAGGGGGA GGGGCTTGAT
    GAGATGCCTC TCAAAGAGCC GTCATTCAGA ATAAACTTCA GATGTAAACC GTCTTTCAGG
    GAGTCAGGAT CACGGAACAT CCGAGAGCCT GCAATAGTGC GACACCACTG GGTCCATCGG
    AGAAGACAAG AGGGCAAATG CAAACAGTGT GGAAAGGGGT TTCAGCAGAA ATTTGCCTTT
    CACAGTAAAG AGATTGTAGC CATCAGCTGC TCCTGGTGCA AACAAGCATA TCATAATAAG
    GTGACATGCT TTATGCTGCA GCAGATAGAA GAACCATGTT CATTAGGAGC TCATGCCGCT
    GTAATAGTAC CTCCTACTTG GATCATACGG GTTCGCCGAC CTCAGTCCTC ACTAAAGTCA
    AGCAAAAAGA AAAAACGGAC ATCTTTCAAA CGTAAATCCA GCAAGAAAGG AGCAGAGGAG
    GCACGATGGA AGCCTTTTAT AGTGAGACCC ATTCCCTCCC AACTCATGAA GCCACTGCTA
    GTGTTTGTAA ATCCAAAGAG TGGAGGAAAC CAGGGAGCTA AAATCATCCA GACCTTTCTT
    TGGTATCTAA ACCCTCGGCA GGTGTTTGAT CTAAGTCAAG GAGGTCCTCA GGAAGGCTTG
    GAAATGTATC GCAAGGTTCA CAACTTGAGA ATTTTGGCCT GTGGGGGGGA TGGCACGGTG
    GGCTGGATCT TATCTGCACT TGATCAGCTG CAATTAAACC CTTCTCCTGC TGTGGCCGTA
    CTTCCCCTGG GCACAGGAAA TGACCTTGCT AGGACGCTAA ACTGGGGTGG GGGCTACACA
    GATGAACCTC TGAGTAAAAT CCTGTCTCAT GTGGAGGATG GGAATATTGT ACAGCTGGAT
    CGGTGGAATC TTGTTGTGAA GCCCAACCCA GAGGCAGGAC CAGAGGAAAG AGACGAGCAG
    GTGACGGACA AGCTCCCTCT AGACGTCTTC AATAACTACT TCAGCCTTGG ATTTGATGCT
    CATGTGACTT TAGAGTTCCA CGAATCTCGA GAGGCTAATC CTGAGAAATT TAACAGCCGG
    TTCAGAAACA AAATGTTCTA TGCAGGGACA GCGTTTTCTG ATTTCTTGAT GGGCAGCTCC
    AAAGACCTGG CTAAGCACAT CAAAGTAGTC TGTGACGGCA CAGATTTAAC CTCAAAGGTC
    CAGGATTTGA AGCTGCAATG CCTGGTCTTC CTAAATATCC CCAGGTACTG TGCGGGCACA
    ATGCCATGGG GCAATCCGAG TGAGCATCAT GACTTTGAGC CTCAGCGTCA TGATGATGGC
    TGCATTGAGG TTATTGGATT CACAATGACA TCACTGGCCA CACTGCAGGT CGGAGGTCAC
    GGTGAGAGGT TAAATCAGTG TCGCGAAGTC ACCCTCACCA CCTTTAAGTC AATCCCCATG
    CAGGTGGACG GGGAGCCTTG TAAACTAGCA CCATCTGTTA TTCACATTTC TTTGCGAAAC
    CAGGCCAACA TGGTGCAGAA AACCAAGAGA CGTACATCTA TACCACTGCT AAACGATCAG
    CAACCGTTTC CAGAACGACT ACGGATCAGA GTGAGCCGTA TCAGCATGCA TGATTATGAA
    GCTTTGCACT ACGACAAGGA GAAGCTCAAA GAAGCTTCGA TTCCACTGGG TCTCATCGTT
    GTCCCTGGAG ACAGTGATTT GGAAACCTGC CGAGCTCACA TCGAGAGACT ACAGGAGGAA
    GGTGACGGGA GCAAATCAAA GGCTTTGTCG TCTCAGAGGC TATCACCGAA ATGGTGCTTT
    CTCGACTCTA CAACTGCTGA TCGATTCTAC AGGATTGATC GAGCTCAAGA GCATCTCAAC
    TACGTGACTG AAATTTCTCA GGATGAGCTG TTTGTTTTGG ACCCAGAGCT GGTGGTCACA
    GAGACTGTTA GCACATCACC TGGCATGCCA GACCTGGTGG ACTCTGGTGA AGTCCAGTCC
    GATCCACACA AATTTGCCTT TCCGTCATCC TCTTCTTCAC CACCAAACAC ACCTGGACCT
    AGGGTTGCAG AGCTGCAAAG GAAGCGTGTT TCCAGTGACA GTTCGGTGGC AGAAGCACTT
    GCACACAGTG ACTCGCTCAG ATCAGCCAAG CCAGCTCTCT GCAGAGCTGG GGGTGTTCAT
    CGCTCAAATA CAACCGCAGC AGATTTCAAA CCTACCAGCA GGTTTGAGAA GAGCGCTGTG
    AACAATGCCA ACTCTGTGTC AGTGGACAGT CTAATTGAAT GTGTGAAAAA GAAGGACCAT
    CAGAAGTTGA AGGAGCTGCA TAAGAAAGGG GCCGACCTGT GTGTGCAGGA CCCTGCTGGA
    CGGACCCTAT TGCACTATGC TGTGGAAGTG GGCAGCAAGG AGATTGTCAA ATACATCATT
    GATAATGCAC CCACCGATAT CCTGGATGTA ACAGAGAAAG AAAATGGAGA GACTGTGTTG
    CACAAAGCAG CCTCGCTGTG TCATAGGACA ATCTGTCATT ACCTAGTGGA AGCAGGAGCA
    TCTCTTATGA AGACAGACTT ACAGGGTGAC ACTCCGAAGC ATCGTGCCGA GAAGGCGAAA
    GATGCTGAAC TTGCGGCATA TCTTGAAAAC CGCCAGCACT ACCAAATGAT CCAGCGTGAG
    GACCAGGAGA CTGCAGTTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa13280
    Clone ID Related Accession (Same CDS sequence) XM_005169032.3
    Accession Version XM_005169032.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3732bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product diacylglycerol kinase zeta isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169032.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
    CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT
    TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG
    CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA
    GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT
    CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC
    CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC
    CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA
    TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA
    CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC
    CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT
    GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG
    ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC
    AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC
    CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA
    ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG
    ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG
    CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC
    ATTGAACAGC TGGAGAAGTT ATTGGATTGG AATGCTCCAC CTCAGGGGGA GGGGCTTGAT
    GAGATGCCTC TCAAAGAGCC GTCATTCAGA ATAAACTTCA GATGTAAACC GTCTTTCAGG
    GAGTCAGGAT CACGGAACAT CCGAGAGCCT GCAATAGTGC GACACCACTG GGTCCATCGG
    AGAAGACAAG AGGGCAAATG CAAACAGTGT GGAAAGGGGT TTCAGCAGAA ATTTGCCTTT
    CACAGTAAAG AGATTGTAGC CATCAGCTGC TCCTGGTGCA AACAAGCATA TCATAATAAG
    GTGACATGCT TTATGCTGCA GCAGATAGAA GAACCATGTT CATTAGGAGC TCATGCCGCT
    GTAATAGTAC CTCCTACTTG GATCATACGG GTTCGCCGAC CTCAGTCCTC ACTAAAGTCA
    AGCAAAAAGA AAAAACGGAC ATCTTTCAAA CGTAAATCCA GCAAGAAAGG AGCAGAGGAG
    GCACGATGGA AGCCTTTTAT AGTGAGACCC ATTCCCTCCC AACTCATGAA GCCACTGCTA
    GTGTTTGTAA ATCCAAAGAG TGGAGGAAAC CAGGGAGCTA AAATCATCCA GACCTTTCTT
    TGGTATCTAA ACCCTCGGCA GGTGTTTGAT CTAAGTCAAG GAGGTCCTCA GGAAGGCTTG
    GAAATGTATC GCAAGGTTCA CAACTTGAGA ATTTTGGCCT GTGGGGGGGA TGGCACGGTG
    GGCTGGATCT TATCTGCACT TGATCAGCTG CAATTAAACC CTTCTCCTGC TGTGGCCGTA
    CTTCCCCTGG GCACAGGAAA TGACCTTGCT AGGACGCTAA ACTGGGGTGG GGGCTACACA
    GATGAACCTC TGAGTAAAAT CCTGTCTCAT GTGGAGGATG GGAATATTGT ACAGCTGGAT
    CGGTGGAATC TTGTTGTGAA GCCCAACCCA GAGGCAGGAC CAGAGGAAAG AGACGAGCAG
    GTGACGGACA AGCTCCCTCT AGACGTCTTC AATAACTACT TCAGCCTTGG ATTTGATGCT
    CATGTGACTT TAGAGTTCCA CGAATCTCGA GAGGCTAATC CTGAGAAATT TAACAGCCGG
    TTCAGAAACA AAATGTTCTA TGCAGGGACA GCGTTTTCTG ATTTCTTGAT GGGCAGCTCC
    AAAGACCTGG CTAAGCACAT CAAAGTAGTC TGTGACGGCA CAGATTTAAC CTCAAAGGTC
    CAGGATTTGA AGCTGCAATG CCTGGTCTTC CTAAATATCC CCAGGTACTG TGCGGGCACA
    ATGCCATGGG GCAATCCGAG TGAGCATCAT GACTTTGAGC CTCAGCGTCA TGATGATGGC
    TGCATTGAGG TTATTGGATT CACAATGACA TCACTGGCCA CACTGCAGGT CGGAGGTCAC
    GGTGAGAGGT TAAATCAGTG TCGCGAAGTC ACCCTCACCA CCTTTAAGTC AATCCCCATG
    CAGGTGGACG GGGAGCCTTG TAAACTAGCA CCATCTGTTA TTCACATTTC TTTGCGAAAC
    CAGGCCAACA TGGTGCAGAA AACCAAGAGA CGTACATCTA TACCACTGCT AAACGACAGT
    CAGCAACCGT TTCCAGAACG ACTACGGATC AGAGTGAGCC GTATCAGCAT GCATGATTAT
    GAAGCTTTGC ACTACGACAA GGAGAAGCTC AAAGAAGCTT CGATTCCACT GGGTCTCATC
    GTTGTCCCTG GAGACAGTGA TTTGGAAACC TGCCGAGCTC ACATCGAGAG ACTACAGGAG
    GACTTTGTGT CGTGTAACCC GTCTCTGCAG CGGCTGGTGC TCCAGGAAGG TGACGGGAGC
    AAATCAAAGG CTTTGTCGTC TCAGAGGCTA TCACCGAAAT GGTGCTTTCT CGACTCTACA
    ACTGCTGATC GATTCTACAG GATTGATCGA GCTCAAGAGC ATCTCAACTA CGTGACTGAA
    ATTTCTCAGG ATGAGCTGTT TGTTTTGGAC CCAGAGCTGG TGGTCACAGA GACTGTTAGC
    ACATCACCTG GCATGCCAGA CCTGGTGGAC TCTGGTGAAG TCCAGTCCGA TCCACACAAA
    TTTGCCTTTC CGTCATCCTC TTCTTCACCA CCAAACACAC CTGGACCTAG GGTTGCAGAG
    CTGCAAAGGA AGCGTGTTTC CAGTGACAGT TCGGTGGCAG AAGCACTTGC ACACAGTGAC
    TCGCTCAGAT CAGCCAAGCC AGCTCTCTGC AGGTTTGAGA AGAGCGCTGT GAACAATGCC
    AACTCTGTGT CAGTGGACAG TCTAATTGAA TGTGTGAAAA AGAAGGACCA TCAGAAGTTG
    AAGGAGCTGC ATAAGAAAGG GGCCGACCTG TGTGTGCAGG ACCCTGCTGG ACGGACCCTA
    TTGCACTATG CTGTGGAAGT GGGCAGCAAG GAGATTGTCA AATACATCAT TGATAATGCA
    CCCACCGATA TCCTGGATGT AACAGAGAAA GAAAATGGAG AGACTGTGTT GCACAAAGCA
    GCCTCGCTGT GTCATAGGAC AATCTGTCAT TACCTAGTGG AAGCAGGAGC ATCTCTTATG
    AAGACAGACT TACAGGGTGA CACTCCGAAG CATCGTGCCG AGAAGGCGAA AGATGCTGAA
    CTTGCGGCAT ATCTTGAAAA CCGCCAGCAC TACCAAATGA TCCAGCGTGA GGACCAGGAG
    ACTGCAGTTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005169089.1
    CDS136..3867
    Translation

    Target ORF information:

    RefSeq Version XM_005169032.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005169032.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
    CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT
    TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG
    CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA
    GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT
    CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC
    CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC
    CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA
    TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA
    CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC
    CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT
    GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG
    ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC
    AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC
    CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA
    ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG
    ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG
    CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC
    ATTGAACAGC TGGAGAAGTT ATTGGATTGG AATGCTCCAC CTCAGGGGGA GGGGCTTGAT
    GAGATGCCTC TCAAAGAGCC GTCATTCAGA ATAAACTTCA GATGTAAACC GTCTTTCAGG
    GAGTCAGGAT CACGGAACAT CCGAGAGCCT GCAATAGTGC GACACCACTG GGTCCATCGG
    AGAAGACAAG AGGGCAAATG CAAACAGTGT GGAAAGGGGT TTCAGCAGAA ATTTGCCTTT
    CACAGTAAAG AGATTGTAGC CATCAGCTGC TCCTGGTGCA AACAAGCATA TCATAATAAG
    GTGACATGCT TTATGCTGCA GCAGATAGAA GAACCATGTT CATTAGGAGC TCATGCCGCT
    GTAATAGTAC CTCCTACTTG GATCATACGG GTTCGCCGAC CTCAGTCCTC ACTAAAGTCA
    AGCAAAAAGA AAAAACGGAC ATCTTTCAAA CGTAAATCCA GCAAGAAAGG AGCAGAGGAG
    GCACGATGGA AGCCTTTTAT AGTGAGACCC ATTCCCTCCC AACTCATGAA GCCACTGCTA
    GTGTTTGTAA ATCCAAAGAG TGGAGGAAAC CAGGGAGCTA AAATCATCCA GACCTTTCTT
    TGGTATCTAA ACCCTCGGCA GGTGTTTGAT CTAAGTCAAG GAGGTCCTCA GGAAGGCTTG
    GAAATGTATC GCAAGGTTCA CAACTTGAGA ATTTTGGCCT GTGGGGGGGA TGGCACGGTG
    GGCTGGATCT TATCTGCACT TGATCAGCTG CAATTAAACC CTTCTCCTGC TGTGGCCGTA
    CTTCCCCTGG GCACAGGAAA TGACCTTGCT AGGACGCTAA ACTGGGGTGG GGGCTACACA
    GATGAACCTC TGAGTAAAAT CCTGTCTCAT GTGGAGGATG GGAATATTGT ACAGCTGGAT
    CGGTGGAATC TTGTTGTGAA GCCCAACCCA GAGGCAGGAC CAGAGGAAAG AGACGAGCAG
    GTGACGGACA AGCTCCCTCT AGACGTCTTC AATAACTACT TCAGCCTTGG ATTTGATGCT
    CATGTGACTT TAGAGTTCCA CGAATCTCGA GAGGCTAATC CTGAGAAATT TAACAGCCGG
    TTCAGAAACA AAATGTTCTA TGCAGGGACA GCGTTTTCTG ATTTCTTGAT GGGCAGCTCC
    AAAGACCTGG CTAAGCACAT CAAAGTAGTC TGTGACGGCA CAGATTTAAC CTCAAAGGTC
    CAGGATTTGA AGCTGCAATG CCTGGTCTTC CTAAATATCC CCAGGTACTG TGCGGGCACA
    ATGCCATGGG GCAATCCGAG TGAGCATCAT GACTTTGAGC CTCAGCGTCA TGATGATGGC
    TGCATTGAGG TTATTGGATT CACAATGACA TCACTGGCCA CACTGCAGGT CGGAGGTCAC
    GGTGAGAGGT TAAATCAGTG TCGCGAAGTC ACCCTCACCA CCTTTAAGTC AATCCCCATG
    CAGGTGGACG GGGAGCCTTG TAAACTAGCA CCATCTGTTA TTCACATTTC TTTGCGAAAC
    CAGGCCAACA TGGTGCAGAA AACCAAGAGA CGTACATCTA TACCACTGCT AAACGACAGT
    CAGCAACCGT TTCCAGAACG ACTACGGATC AGAGTGAGCC GTATCAGCAT GCATGATTAT
    GAAGCTTTGC ACTACGACAA GGAGAAGCTC AAAGAAGCTT CGATTCCACT GGGTCTCATC
    GTTGTCCCTG GAGACAGTGA TTTGGAAACC TGCCGAGCTC ACATCGAGAG ACTACAGGAG
    GACTTTGTGT CGTGTAACCC GTCTCTGCAG CGGCTGGTGC TCCAGGAAGG TGACGGGAGC
    AAATCAAAGG CTTTGTCGTC TCAGAGGCTA TCACCGAAAT GGTGCTTTCT CGACTCTACA
    ACTGCTGATC GATTCTACAG GATTGATCGA GCTCAAGAGC ATCTCAACTA CGTGACTGAA
    ATTTCTCAGG ATGAGCTGTT TGTTTTGGAC CCAGAGCTGG TGGTCACAGA GACTGTTAGC
    ACATCACCTG GCATGCCAGA CCTGGTGGAC TCTGGTGAAG TCCAGTCCGA TCCACACAAA
    TTTGCCTTTC CGTCATCCTC TTCTTCACCA CCAAACACAC CTGGACCTAG GGTTGCAGAG
    CTGCAAAGGA AGCGTGTTTC CAGTGACAGT TCGGTGGCAG AAGCACTTGC ACACAGTGAC
    TCGCTCAGAT CAGCCAAGCC AGCTCTCTGC AGGTTTGAGA AGAGCGCTGT GAACAATGCC
    AACTCTGTGT CAGTGGACAG TCTAATTGAA TGTGTGAAAA AGAAGGACCA TCAGAAGTTG
    AAGGAGCTGC ATAAGAAAGG GGCCGACCTG TGTGTGCAGG ACCCTGCTGG ACGGACCCTA
    TTGCACTATG CTGTGGAAGT GGGCAGCAAG GAGATTGTCA AATACATCAT TGATAATGCA
    CCCACCGATA TCCTGGATGT AACAGAGAAA GAAAATGGAG AGACTGTGTT GCACAAAGCA
    GCCTCGCTGT GTCATAGGAC AATCTGTCAT TACCTAGTGG AAGCAGGAGC ATCTCTTATG
    AAGACAGACT TACAGGGTGA CACTCCGAAG CATCGTGCCG AGAAGGCGAA AGATGCTGAA
    CTTGCGGCAT ATCTTGAAAA CCGCCAGCAC TACCAAATGA TCCAGCGTGA GGACCAGGAG
    ACTGCAGTTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa13281
    Clone ID Related Accession (Same CDS sequence) XM_005169033.3 , XM_005169033.4
    Accession Version XM_005169033.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3717bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product diacylglycerol kinase zeta isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169033.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
    CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT
    TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG
    CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA
    GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT
    CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC
    CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC
    CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA
    TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA
    CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC
    CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT
    GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG
    ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC
    AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC
    CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA
    ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG
    ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG
    CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC
    ATTGAACAGC TGGAGAAGAT AAACTTCAGA TGTAAACCGT CTTTCAGGGA GTCAGGATCA
    CGGAACATCC GAGAGCCTGC AATAGTGCGA CACCACTGGG TCCATCGGAG AAGACAAGAG
    GGCAAATGCA AACAGTGTGG AAAGGGGTTT CAGCAGAAAT TTGCCTTTCA CAGTAAAGAG
    ATTGTAGCCA TCAGCTGCTC CTGGTGCAAA CAAGCATATC ATAATAAGGT GACATGCTTT
    ATGCTGCAGC AGATAGAAGA ACCATGTTCA TTAGGAGCTC ATGCCGCTGT AATAGTACCT
    CCTACTTGGA TCATACGGGT TCGCCGACCT CAGTCCTCAC TAAAGTCAAG CAAAAAGAAA
    AAACGGACAT CTTTCAAACG TAAATCCAGC AAGAAAGGAG CAGAGGAGGC ACGATGGAAG
    CCTTTTATAG TGAGACCCAT TCCCTCCCAA CTCATGAAGC CACTGCTAGT GTTTGTAAAT
    CCAAAGAGTG GAGGAAACCA GGGAGCTAAA ATCATCCAGA CCTTTCTTTG GTATCTAAAC
    CCTCGGCAGG TGTTTGATCT AAGTCAAGGA GGTCCTCAGG AAGGCTTGGA AATGTATCGC
    AAGGTTCACA ACTTGAGAAT TTTGGCCTGT GGGGGGGATG GCACGGTGGG CTGGATCTTA
    TCTGCACTTG ATCAGCTGCA ATTAAACCCT TCTCCTGCTG TGGCCGTACT TCCCCTGGGC
    ACAGGAAATG ACCTTGCTAG GACGCTAAAC TGGGGTGGGG GCTACACAGA TGAACCTCTG
    AGTAAAATCC TGTCTCATGT GGAGGATGGG AATATTGTAC AGCTGGATCG GTGGAATCTT
    GTTGTGAAGC CCAACCCAGA GGCAGGACCA GAGGAAAGAG ACGAGCAGGT GACGGACAAG
    CTCCCTCTAG ACGTCTTCAA TAACTACTTC AGCCTTGGAT TTGATGCTCA TGTGACTTTA
    GAGTTCCACG AATCTCGAGA GGCTAATCCT GAGAAATTTA ACAGCCGGTT CAGAAACAAA
    ATGTTCTATG CAGGGACAGC GTTTTCTGAT TTCTTGATGG GCAGCTCCAA AGACCTGGCT
    AAGCACATCA AAGTAGTCTG TGACGGCACA GATTTAACCT CAAAGGTCCA GGATTTGAAG
    CTGCAATGCC TGGTCTTCCT AAATATCCCC AGGTACTGTG CGGGCACAAT GCCATGGGGC
    AATCCGAGTG AGCATCATGA CTTTGAGCCT CAGCGTCATG ATGATGGCTG CATTGAGGTT
    ATTGGATTCA CAATGACATC ACTGGCCACA CTGCAGGTCG GAGGTCACGG TGAGAGGTTA
    AATCAGTGTC GCGAAGTCAC CCTCACCACC TTTAAGTCAA TCCCCATGCA GGTGGACGGG
    GAGCCTTGTA AACTAGCACC ATCTGTTATT CACATTTCTT TGCGAAACCA GGCCAACATG
    GTGCAGAAAA CCAAGAGACG TACATCTATA CCACTGCTAA ACGACAGTCA GCAACCGTTT
    CCAGAACGAC TACGGATCAG AGTGAGCCGT ATCAGCATGC ATGATTATGA AGCTTTGCAC
    TACGACAAGG AGAAGCTCAA AGAAGCTTCG ATTCCACTGG GTCTCATCGT TGTCCCTGGA
    GACAGTGATT TGGAAACCTG CCGAGCTCAC ATCGAGAGAC TACAGGAGGA CTTTGTGTCG
    TGTAACCCGT CTCTGCAGCG GCTGGTGCTC CAGGAAGGTG ACGGGAGCAA ATCAAAGGCT
    TTGTCGTCTC AGAGGCTATC ACCGAAATGG TGCTTTCTCG ACTCTACAAC TGCTGATCGA
    TTCTACAGGA TTGATCGAGC TCAAGAGCAT CTCAACTACG TGACTGAAAT TTCTCAGGAT
    GAGCTGTTTG TTTTGGACCC AGAGCTGGTG GTCACAGAGA CTGTTAGCAC ATCACCTGGC
    ATGCCAGACC TGGTGGACTC TGGTGAAGTC CAGTCCGATC CACACAAATT TGCCTTTCCG
    TCATCCTCTT CTTCACCACC AAACACACCT GGACCTAGGG TTGCAGAGCT GCAAAGGAAG
    CGTGTTTCCA GTGACAGTTC GGTGGCAGAA GCACTTGCAC ACAGTGACTC GCTCAGATCA
    GCCAAGCCAG CTCTCTGCAG AGCTGGGGGT GTTCATCGCT CAAATACAAC CGCAGCAGAT
    TTCAAACCTA CCAGCAGGTT TGAGAAGAGC GCTGTGAACA ATGCCAACTC TGTGTCAGTG
    GACAGTCTAA TTGAATGTGT GAAAAAGAAG GACCATCAGA AGTTGAAGGA GCTGCATAAG
    AAAGGGGCCG ACCTGTGTGT GCAGGACCCT GCTGGACGGA CCCTATTGCA CTATGCTGTG
    GAAGTGGGCA GCAAGGAGAT TGTCAAATAC ATCATTGATA ATGCACCCAC CGATATCCTG
    GATGTAACAG AGAAAGAAAA TGGAGAGACT GTGTTGCACA AAGCAGCCTC GCTGTGTCAT
    AGGACAATCT GTCATTACCT AGTGGAAGCA GGAGCATCTC TTATGAAGAC AGACTTACAG
    GGTGACACTC CGAAGCATCG TGCCGAGAAG GCGAAAGATG CTGAACTTGC GGCATATCTT
    GAAAACCGCC AGCACTACCA AATGATCCAG CGTGAGGACC AGGAGACTGC AGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005169090.1
    CDS136..3852
    Translation

    Target ORF information:

    RefSeq Version XM_005169033.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005169033.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
    CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT
    TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG
    CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA
    GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT
    CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC
    CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC
    CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA
    TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA
    CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC
    CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT
    GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG
    ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC
    AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC
    CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA
    ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG
    ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG
    CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC
    ATTGAACAGC TGGAGAAGAT AAACTTCAGA TGTAAACCGT CTTTCAGGGA GTCAGGATCA
    CGGAACATCC GAGAGCCTGC AATAGTGCGA CACCACTGGG TCCATCGGAG AAGACAAGAG
    GGCAAATGCA AACAGTGTGG AAAGGGGTTT CAGCAGAAAT TTGCCTTTCA CAGTAAAGAG
    ATTGTAGCCA TCAGCTGCTC CTGGTGCAAA CAAGCATATC ATAATAAGGT GACATGCTTT
    ATGCTGCAGC AGATAGAAGA ACCATGTTCA TTAGGAGCTC ATGCCGCTGT AATAGTACCT
    CCTACTTGGA TCATACGGGT TCGCCGACCT CAGTCCTCAC TAAAGTCAAG CAAAAAGAAA
    AAACGGACAT CTTTCAAACG TAAATCCAGC AAGAAAGGAG CAGAGGAGGC ACGATGGAAG
    CCTTTTATAG TGAGACCCAT TCCCTCCCAA CTCATGAAGC CACTGCTAGT GTTTGTAAAT
    CCAAAGAGTG GAGGAAACCA GGGAGCTAAA ATCATCCAGA CCTTTCTTTG GTATCTAAAC
    CCTCGGCAGG TGTTTGATCT AAGTCAAGGA GGTCCTCAGG AAGGCTTGGA AATGTATCGC
    AAGGTTCACA ACTTGAGAAT TTTGGCCTGT GGGGGGGATG GCACGGTGGG CTGGATCTTA
    TCTGCACTTG ATCAGCTGCA ATTAAACCCT TCTCCTGCTG TGGCCGTACT TCCCCTGGGC
    ACAGGAAATG ACCTTGCTAG GACGCTAAAC TGGGGTGGGG GCTACACAGA TGAACCTCTG
    AGTAAAATCC TGTCTCATGT GGAGGATGGG AATATTGTAC AGCTGGATCG GTGGAATCTT
    GTTGTGAAGC CCAACCCAGA GGCAGGACCA GAGGAAAGAG ACGAGCAGGT GACGGACAAG
    CTCCCTCTAG ACGTCTTCAA TAACTACTTC AGCCTTGGAT TTGATGCTCA TGTGACTTTA
    GAGTTCCACG AATCTCGAGA GGCTAATCCT GAGAAATTTA ACAGCCGGTT CAGAAACAAA
    ATGTTCTATG CAGGGACAGC GTTTTCTGAT TTCTTGATGG GCAGCTCCAA AGACCTGGCT
    AAGCACATCA AAGTAGTCTG TGACGGCACA GATTTAACCT CAAAGGTCCA GGATTTGAAG
    CTGCAATGCC TGGTCTTCCT AAATATCCCC AGGTACTGTG CGGGCACAAT GCCATGGGGC
    AATCCGAGTG AGCATCATGA CTTTGAGCCT CAGCGTCATG ATGATGGCTG CATTGAGGTT
    ATTGGATTCA CAATGACATC ACTGGCCACA CTGCAGGTCG GAGGTCACGG TGAGAGGTTA
    AATCAGTGTC GCGAAGTCAC CCTCACCACC TTTAAGTCAA TCCCCATGCA GGTGGACGGG
    GAGCCTTGTA AACTAGCACC ATCTGTTATT CACATTTCTT TGCGAAACCA GGCCAACATG
    GTGCAGAAAA CCAAGAGACG TACATCTATA CCACTGCTAA ACGACAGTCA GCAACCGTTT
    CCAGAACGAC TACGGATCAG AGTGAGCCGT ATCAGCATGC ATGATTATGA AGCTTTGCAC
    TACGACAAGG AGAAGCTCAA AGAAGCTTCG ATTCCACTGG GTCTCATCGT TGTCCCTGGA
    GACAGTGATT TGGAAACCTG CCGAGCTCAC ATCGAGAGAC TACAGGAGGA CTTTGTGTCG
    TGTAACCCGT CTCTGCAGCG GCTGGTGCTC CAGGAAGGTG ACGGGAGCAA ATCAAAGGCT
    TTGTCGTCTC AGAGGCTATC ACCGAAATGG TGCTTTCTCG ACTCTACAAC TGCTGATCGA
    TTCTACAGGA TTGATCGAGC TCAAGAGCAT CTCAACTACG TGACTGAAAT TTCTCAGGAT
    GAGCTGTTTG TTTTGGACCC AGAGCTGGTG GTCACAGAGA CTGTTAGCAC ATCACCTGGC
    ATGCCAGACC TGGTGGACTC TGGTGAAGTC CAGTCCGATC CACACAAATT TGCCTTTCCG
    TCATCCTCTT CTTCACCACC AAACACACCT GGACCTAGGG TTGCAGAGCT GCAAAGGAAG
    CGTGTTTCCA GTGACAGTTC GGTGGCAGAA GCACTTGCAC ACAGTGACTC GCTCAGATCA
    GCCAAGCCAG CTCTCTGCAG AGCTGGGGGT GTTCATCGCT CAAATACAAC CGCAGCAGAT
    TTCAAACCTA CCAGCAGGTT TGAGAAGAGC GCTGTGAACA ATGCCAACTC TGTGTCAGTG
    GACAGTCTAA TTGAATGTGT GAAAAAGAAG GACCATCAGA AGTTGAAGGA GCTGCATAAG
    AAAGGGGCCG ACCTGTGTGT GCAGGACCCT GCTGGACGGA CCCTATTGCA CTATGCTGTG
    GAAGTGGGCA GCAAGGAGAT TGTCAAATAC ATCATTGATA ATGCACCCAC CGATATCCTG
    GATGTAACAG AGAAAGAAAA TGGAGAGACT GTGTTGCACA AAGCAGCCTC GCTGTGTCAT
    AGGACAATCT GTCATTACCT AGTGGAAGCA GGAGCATCTC TTATGAAGAC AGACTTACAG
    GGTGACACTC CGAAGCATCG TGCCGAGAAG GCGAAAGATG CTGAACTTGC GGCATATCTT
    GAAAACCGCC AGCACTACCA AATGATCCAG CGTGAGGACC AGGAGACTGC AGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa13281
    Clone ID Related Accession (Same CDS sequence) XM_005169033.3 , XM_005169033.4
    Accession Version XM_005169033.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3717bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product diacylglycerol kinase zeta isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005169033.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
    CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT
    TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG
    CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA
    GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT
    CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC
    CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC
    CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA
    TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA
    CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC
    CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT
    GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG
    ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC
    AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC
    CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA
    ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG
    ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG
    CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC
    ATTGAACAGC TGGAGAAGAT AAACTTCAGA TGTAAACCGT CTTTCAGGGA GTCAGGATCA
    CGGAACATCC GAGAGCCTGC AATAGTGCGA CACCACTGGG TCCATCGGAG AAGACAAGAG
    GGCAAATGCA AACAGTGTGG AAAGGGGTTT CAGCAGAAAT TTGCCTTTCA CAGTAAAGAG
    ATTGTAGCCA TCAGCTGCTC CTGGTGCAAA CAAGCATATC ATAATAAGGT GACATGCTTT
    ATGCTGCAGC AGATAGAAGA ACCATGTTCA TTAGGAGCTC ATGCCGCTGT AATAGTACCT
    CCTACTTGGA TCATACGGGT TCGCCGACCT CAGTCCTCAC TAAAGTCAAG CAAAAAGAAA
    AAACGGACAT CTTTCAAACG TAAATCCAGC AAGAAAGGAG CAGAGGAGGC ACGATGGAAG
    CCTTTTATAG TGAGACCCAT TCCCTCCCAA CTCATGAAGC CACTGCTAGT GTTTGTAAAT
    CCAAAGAGTG GAGGAAACCA GGGAGCTAAA ATCATCCAGA CCTTTCTTTG GTATCTAAAC
    CCTCGGCAGG TGTTTGATCT AAGTCAAGGA GGTCCTCAGG AAGGCTTGGA AATGTATCGC
    AAGGTTCACA ACTTGAGAAT TTTGGCCTGT GGGGGGGATG GCACGGTGGG CTGGATCTTA
    TCTGCACTTG ATCAGCTGCA ATTAAACCCT TCTCCTGCTG TGGCCGTACT TCCCCTGGGC
    ACAGGAAATG ACCTTGCTAG GACGCTAAAC TGGGGTGGGG GCTACACAGA TGAACCTCTG
    AGTAAAATCC TGTCTCATGT GGAGGATGGG AATATTGTAC AGCTGGATCG GTGGAATCTT
    GTTGTGAAGC CCAACCCAGA GGCAGGACCA GAGGAAAGAG ACGAGCAGGT GACGGACAAG
    CTCCCTCTAG ACGTCTTCAA TAACTACTTC AGCCTTGGAT TTGATGCTCA TGTGACTTTA
    GAGTTCCACG AATCTCGAGA GGCTAATCCT GAGAAATTTA ACAGCCGGTT CAGAAACAAA
    ATGTTCTATG CAGGGACAGC GTTTTCTGAT TTCTTGATGG GCAGCTCCAA AGACCTGGCT
    AAGCACATCA AAGTAGTCTG TGACGGCACA GATTTAACCT CAAAGGTCCA GGATTTGAAG
    CTGCAATGCC TGGTCTTCCT AAATATCCCC AGGTACTGTG CGGGCACAAT GCCATGGGGC
    AATCCGAGTG AGCATCATGA CTTTGAGCCT CAGCGTCATG ATGATGGCTG CATTGAGGTT
    ATTGGATTCA CAATGACATC ACTGGCCACA CTGCAGGTCG GAGGTCACGG TGAGAGGTTA
    AATCAGTGTC GCGAAGTCAC CCTCACCACC TTTAAGTCAA TCCCCATGCA GGTGGACGGG
    GAGCCTTGTA AACTAGCACC ATCTGTTATT CACATTTCTT TGCGAAACCA GGCCAACATG
    GTGCAGAAAA CCAAGAGACG TACATCTATA CCACTGCTAA ACGACAGTCA GCAACCGTTT
    CCAGAACGAC TACGGATCAG AGTGAGCCGT ATCAGCATGC ATGATTATGA AGCTTTGCAC
    TACGACAAGG AGAAGCTCAA AGAAGCTTCG ATTCCACTGG GTCTCATCGT TGTCCCTGGA
    GACAGTGATT TGGAAACCTG CCGAGCTCAC ATCGAGAGAC TACAGGAGGA CTTTGTGTCG
    TGTAACCCGT CTCTGCAGCG GCTGGTGCTC CAGGAAGGTG ACGGGAGCAA ATCAAAGGCT
    TTGTCGTCTC AGAGGCTATC ACCGAAATGG TGCTTTCTCG ACTCTACAAC TGCTGATCGA
    TTCTACAGGA TTGATCGAGC TCAAGAGCAT CTCAACTACG TGACTGAAAT TTCTCAGGAT
    GAGCTGTTTG TTTTGGACCC AGAGCTGGTG GTCACAGAGA CTGTTAGCAC ATCACCTGGC
    ATGCCAGACC TGGTGGACTC TGGTGAAGTC CAGTCCGATC CACACAAATT TGCCTTTCCG
    TCATCCTCTT CTTCACCACC AAACACACCT GGACCTAGGG TTGCAGAGCT GCAAAGGAAG
    CGTGTTTCCA GTGACAGTTC GGTGGCAGAA GCACTTGCAC ACAGTGACTC GCTCAGATCA
    GCCAAGCCAG CTCTCTGCAG AGCTGGGGGT GTTCATCGCT CAAATACAAC CGCAGCAGAT
    TTCAAACCTA CCAGCAGGTT TGAGAAGAGC GCTGTGAACA ATGCCAACTC TGTGTCAGTG
    GACAGTCTAA TTGAATGTGT GAAAAAGAAG GACCATCAGA AGTTGAAGGA GCTGCATAAG
    AAAGGGGCCG ACCTGTGTGT GCAGGACCCT GCTGGACGGA CCCTATTGCA CTATGCTGTG
    GAAGTGGGCA GCAAGGAGAT TGTCAAATAC ATCATTGATA ATGCACCCAC CGATATCCTG
    GATGTAACAG AGAAAGAAAA TGGAGAGACT GTGTTGCACA AAGCAGCCTC GCTGTGTCAT
    AGGACAATCT GTCATTACCT AGTGGAAGCA GGAGCATCTC TTATGAAGAC AGACTTACAG
    GGTGACACTC CGAAGCATCG TGCCGAGAAG GCGAAAGATG CTGAACTTGC GGCATATCTT
    GAAAACCGCC AGCACTACCA AATGATCCAG CGTGAGGACC AGGAGACTGC AGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005169090.1
    CDS171..3887
    Translation

    Target ORF information:

    RefSeq Version XM_005169033.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005169033.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
    CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT
    TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG
    CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA
    GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT
    CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC
    CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC
    CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA
    TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA
    CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC
    CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT
    GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG
    ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC
    AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC
    CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA
    ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG
    ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG
    CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC
    ATTGAACAGC TGGAGAAGAT AAACTTCAGA TGTAAACCGT CTTTCAGGGA GTCAGGATCA
    CGGAACATCC GAGAGCCTGC AATAGTGCGA CACCACTGGG TCCATCGGAG AAGACAAGAG
    GGCAAATGCA AACAGTGTGG AAAGGGGTTT CAGCAGAAAT TTGCCTTTCA CAGTAAAGAG
    ATTGTAGCCA TCAGCTGCTC CTGGTGCAAA CAAGCATATC ATAATAAGGT GACATGCTTT
    ATGCTGCAGC AGATAGAAGA ACCATGTTCA TTAGGAGCTC ATGCCGCTGT AATAGTACCT
    CCTACTTGGA TCATACGGGT TCGCCGACCT CAGTCCTCAC TAAAGTCAAG CAAAAAGAAA
    AAACGGACAT CTTTCAAACG TAAATCCAGC AAGAAAGGAG CAGAGGAGGC ACGATGGAAG
    CCTTTTATAG TGAGACCCAT TCCCTCCCAA CTCATGAAGC CACTGCTAGT GTTTGTAAAT
    CCAAAGAGTG GAGGAAACCA GGGAGCTAAA ATCATCCAGA CCTTTCTTTG GTATCTAAAC
    CCTCGGCAGG TGTTTGATCT AAGTCAAGGA GGTCCTCAGG AAGGCTTGGA AATGTATCGC
    AAGGTTCACA ACTTGAGAAT TTTGGCCTGT GGGGGGGATG GCACGGTGGG CTGGATCTTA
    TCTGCACTTG ATCAGCTGCA ATTAAACCCT TCTCCTGCTG TGGCCGTACT TCCCCTGGGC
    ACAGGAAATG ACCTTGCTAG GACGCTAAAC TGGGGTGGGG GCTACACAGA TGAACCTCTG
    AGTAAAATCC TGTCTCATGT GGAGGATGGG AATATTGTAC AGCTGGATCG GTGGAATCTT
    GTTGTGAAGC CCAACCCAGA GGCAGGACCA GAGGAAAGAG ACGAGCAGGT GACGGACAAG
    CTCCCTCTAG ACGTCTTCAA TAACTACTTC AGCCTTGGAT TTGATGCTCA TGTGACTTTA
    GAGTTCCACG AATCTCGAGA GGCTAATCCT GAGAAATTTA ACAGCCGGTT CAGAAACAAA
    ATGTTCTATG CAGGGACAGC GTTTTCTGAT TTCTTGATGG GCAGCTCCAA AGACCTGGCT
    AAGCACATCA AAGTAGTCTG TGACGGCACA GATTTAACCT CAAAGGTCCA GGATTTGAAG
    CTGCAATGCC TGGTCTTCCT AAATATCCCC AGGTACTGTG CGGGCACAAT GCCATGGGGC
    AATCCGAGTG AGCATCATGA CTTTGAGCCT CAGCGTCATG ATGATGGCTG CATTGAGGTT
    ATTGGATTCA CAATGACATC ACTGGCCACA CTGCAGGTCG GAGGTCACGG TGAGAGGTTA
    AATCAGTGTC GCGAAGTCAC CCTCACCACC TTTAAGTCAA TCCCCATGCA GGTGGACGGG
    GAGCCTTGTA AACTAGCACC ATCTGTTATT CACATTTCTT TGCGAAACCA GGCCAACATG
    GTGCAGAAAA CCAAGAGACG TACATCTATA CCACTGCTAA ACGACAGTCA GCAACCGTTT
    CCAGAACGAC TACGGATCAG AGTGAGCCGT ATCAGCATGC ATGATTATGA AGCTTTGCAC
    TACGACAAGG AGAAGCTCAA AGAAGCTTCG ATTCCACTGG GTCTCATCGT TGTCCCTGGA
    GACAGTGATT TGGAAACCTG CCGAGCTCAC ATCGAGAGAC TACAGGAGGA CTTTGTGTCG
    TGTAACCCGT CTCTGCAGCG GCTGGTGCTC CAGGAAGGTG ACGGGAGCAA ATCAAAGGCT
    TTGTCGTCTC AGAGGCTATC ACCGAAATGG TGCTTTCTCG ACTCTACAAC TGCTGATCGA
    TTCTACAGGA TTGATCGAGC TCAAGAGCAT CTCAACTACG TGACTGAAAT TTCTCAGGAT
    GAGCTGTTTG TTTTGGACCC AGAGCTGGTG GTCACAGAGA CTGTTAGCAC ATCACCTGGC
    ATGCCAGACC TGGTGGACTC TGGTGAAGTC CAGTCCGATC CACACAAATT TGCCTTTCCG
    TCATCCTCTT CTTCACCACC AAACACACCT GGACCTAGGG TTGCAGAGCT GCAAAGGAAG
    CGTGTTTCCA GTGACAGTTC GGTGGCAGAA GCACTTGCAC ACAGTGACTC GCTCAGATCA
    GCCAAGCCAG CTCTCTGCAG AGCTGGGGGT GTTCATCGCT CAAATACAAC CGCAGCAGAT
    TTCAAACCTA CCAGCAGGTT TGAGAAGAGC GCTGTGAACA ATGCCAACTC TGTGTCAGTG
    GACAGTCTAA TTGAATGTGT GAAAAAGAAG GACCATCAGA AGTTGAAGGA GCTGCATAAG
    AAAGGGGCCG ACCTGTGTGT GCAGGACCCT GCTGGACGGA CCCTATTGCA CTATGCTGTG
    GAAGTGGGCA GCAAGGAGAT TGTCAAATAC ATCATTGATA ATGCACCCAC CGATATCCTG
    GATGTAACAG AGAAAGAAAA TGGAGAGACT GTGTTGCACA AAGCAGCCTC GCTGTGTCAT
    AGGACAATCT GTCATTACCT AGTGGAAGCA GGAGCATCTC TTATGAAGAC AGACTTACAG
    GGTGACACTC CGAAGCATCG TGCCGAGAAG GCGAAAGATG CTGAACTTGC GGCATATCTT
    GAAAACCGCC AGCACTACCA AATGATCCAG CGTGAGGACC AGGAGACTGC AGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa13282
    Clone ID Related Accession (Same CDS sequence) XM_005169034.3 , XM_005169034.4
    Accession Version XM_005169034.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3669bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product diacylglycerol kinase zeta isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169034.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
    CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT
    TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG
    CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA
    GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT
    CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC
    CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC
    CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA
    TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA
    CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC
    CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT
    GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG
    ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC
    AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC
    CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA
    ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG
    ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG
    CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC
    ATTGAACAGC TGGAGAAGAT AAACTTCAGA TGTAAACCGT CTTTCAGGGA GTCAGGATCA
    CGGAACATCC GAGAGCCTGC AATAGTGCGA CACCACTGGG TCCATCGGAG AAGACAAGAG
    GGCAAATGCA AACAGTGTGG AAAGGGGTTT CAGCAGAAAT TTGCCTTTCA CAGTAAAGAG
    ATTGTAGCCA TCAGCTGCTC CTGGTGCAAA CAAGCATATC ATAATAAGGT GACATGCTTT
    ATGCTGCAGC AGATAGAAGA ACCATGTTCA TTAGGAGCTC ATGCCGCTGT AATAGTACCT
    CCTACTTGGA TCATACGGGT TCGCCGACCT CAGTCCTCAC TAAAGTCAAG CAAAAAGAAA
    AAACGGACAT CTTTCAAACG TAAATCCAGC AAGAAAGGAG CAGAGGAGGC ACGATGGAAG
    CCTTTTATAG TGAGACCCAT TCCCTCCCAA CTCATGAAGC CACTGCTAGT GTTTGTAAAT
    CCAAAGAGTG GAGGAAACCA GGGAGCTAAA ATCATCCAGA CCTTTCTTTG GTATCTAAAC
    CCTCGGCAGG TGTTTGATCT AAGTCAAGGA GGTCCTCAGG AAGGCTTGGA AATGTATCGC
    AAGGTTCACA ACTTGAGAAT TTTGGCCTGT GGGGGGGATG GCACGGTGGG CTGGATCTTA
    TCTGCACTTG ATCAGCTGCA ATTAAACCCT TCTCCTGCTG TGGCCGTACT TCCCCTGGGC
    ACAGGAAATG ACCTTGCTAG GACGCTAAAC TGGGGTGGGG GCTACACAGA TGAACCTCTG
    AGTAAAATCC TGTCTCATGT GGAGGATGGG AATATTGTAC AGCTGGATCG GTGGAATCTT
    GTTGTGAAGC CCAACCCAGA GGCAGGACCA GAGGAAAGAG ACGAGCAGGT GACGGACAAG
    CTCCCTCTAG ACGTCTTCAA TAACTACTTC AGCCTTGGAT TTGATGCTCA TGTGACTTTA
    GAGTTCCACG AATCTCGAGA GGCTAATCCT GAGAAATTTA ACAGCCGGTT CAGAAACAAA
    ATGTTCTATG CAGGGACAGC GTTTTCTGAT TTCTTGATGG GCAGCTCCAA AGACCTGGCT
    AAGCACATCA AAGTAGTCTG TGACGGCACA GATTTAACCT CAAAGGTCCA GGATTTGAAG
    CTGCAATGCC TGGTCTTCCT AAATATCCCC AGGTACTGTG CGGGCACAAT GCCATGGGGC
    AATCCGAGTG AGCATCATGA CTTTGAGCCT CAGCGTCATG ATGATGGCTG CATTGAGGTT
    ATTGGATTCA CAATGACATC ACTGGCCACA CTGCAGGTCG GAGGTCACGG TGAGAGGTTA
    AATCAGTGTC GCGAAGTCAC CCTCACCACC TTTAAGTCAA TCCCCATGCA GGTGGACGGG
    GAGCCTTGTA AACTAGCACC ATCTGTTATT CACATTTCTT TGCGAAACCA GGCCAACATG
    GTGCAGAAAA CCAAGAGACG TACATCTATA CCACTGCTAA ACGATCAGCA ACCGTTTCCA
    GAACGACTAC GGATCAGAGT GAGCCGTATC AGCATGCATG ATTATGAAGC TTTGCACTAC
    GACAAGGAGA AGCTCAAAGA AGCTTCGATT CCACTGGGTC TCATCGTTGT CCCTGGAGAC
    AGTGATTTGG AAACCTGCCG AGCTCACATC GAGAGACTAC AGGAGGAAGG TGACGGGAGC
    AAATCAAAGG CTTTGTCGTC TCAGAGGCTA TCACCGAAAT GGTGCTTTCT CGACTCTACA
    ACTGCTGATC GATTCTACAG GATTGATCGA GCTCAAGAGC ATCTCAACTA CGTGACTGAA
    ATTTCTCAGG ATGAGCTGTT TGTTTTGGAC CCAGAGCTGG TGGTCACAGA GACTGTTAGC
    ACATCACCTG GCATGCCAGA CCTGGTGGAC TCTGGTGAAG TCCAGTCCGA TCCACACAAA
    TTTGCCTTTC CGTCATCCTC TTCTTCACCA CCAAACACAC CTGGACCTAG GGTTGCAGAG
    CTGCAAAGGA AGCGTGTTTC CAGTGACAGT TCGGTGGCAG AAGCACTTGC ACACAGTGAC
    TCGCTCAGAT CAGCCAAGCC AGCTCTCTGC AGAGCTGGGG GTGTTCATCG CTCAAATACA
    ACCGCAGCAG ATTTCAAACC TACCAGCAGG TTTGAGAAGA GCGCTGTGAA CAATGCCAAC
    TCTGTGTCAG TGGACAGTCT AATTGAATGT GTGAAAAAGA AGGACCATCA GAAGTTGAAG
    GAGCTGCATA AGAAAGGGGC CGACCTGTGT GTGCAGGACC CTGCTGGACG GACCCTATTG
    CACTATGCTG TGGAAGTGGG CAGCAAGGAG ATTGTCAAAT ACATCATTGA TAATGCACCC
    ACCGATATCC TGGATGTAAC AGAGAAAGAA AATGGAGAGA CTGTGTTGCA CAAAGCAGCC
    TCGCTGTGTC ATAGGACAAT CTGTCATTAC CTAGTGGAAG CAGGAGCATC TCTTATGAAG
    ACAGACTTAC AGGGTGACAC TCCGAAGCAT CGTGCCGAGA AGGCGAAAGA TGCTGAACTT
    GCGGCATATC TTGAAAACCG CCAGCACTAC CAAATGATCC AGCGTGAGGA CCAGGAGACT
    GCAGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005169091.1
    CDS135..3803
    Translation

    Target ORF information:

    RefSeq Version XM_005169034.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005169034.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
    CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT
    TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG
    CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA
    GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT
    CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC
    CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC
    CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA
    TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA
    CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC
    CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT
    GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG
    ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC
    AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC
    CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA
    ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG
    ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG
    CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC
    ATTGAACAGC TGGAGAAGAT AAACTTCAGA TGTAAACCGT CTTTCAGGGA GTCAGGATCA
    CGGAACATCC GAGAGCCTGC AATAGTGCGA CACCACTGGG TCCATCGGAG AAGACAAGAG
    GGCAAATGCA AACAGTGTGG AAAGGGGTTT CAGCAGAAAT TTGCCTTTCA CAGTAAAGAG
    ATTGTAGCCA TCAGCTGCTC CTGGTGCAAA CAAGCATATC ATAATAAGGT GACATGCTTT
    ATGCTGCAGC AGATAGAAGA ACCATGTTCA TTAGGAGCTC ATGCCGCTGT AATAGTACCT
    CCTACTTGGA TCATACGGGT TCGCCGACCT CAGTCCTCAC TAAAGTCAAG CAAAAAGAAA
    AAACGGACAT CTTTCAAACG TAAATCCAGC AAGAAAGGAG CAGAGGAGGC ACGATGGAAG
    CCTTTTATAG TGAGACCCAT TCCCTCCCAA CTCATGAAGC CACTGCTAGT GTTTGTAAAT
    CCAAAGAGTG GAGGAAACCA GGGAGCTAAA ATCATCCAGA CCTTTCTTTG GTATCTAAAC
    CCTCGGCAGG TGTTTGATCT AAGTCAAGGA GGTCCTCAGG AAGGCTTGGA AATGTATCGC
    AAGGTTCACA ACTTGAGAAT TTTGGCCTGT GGGGGGGATG GCACGGTGGG CTGGATCTTA
    TCTGCACTTG ATCAGCTGCA ATTAAACCCT TCTCCTGCTG TGGCCGTACT TCCCCTGGGC
    ACAGGAAATG ACCTTGCTAG GACGCTAAAC TGGGGTGGGG GCTACACAGA TGAACCTCTG
    AGTAAAATCC TGTCTCATGT GGAGGATGGG AATATTGTAC AGCTGGATCG GTGGAATCTT
    GTTGTGAAGC CCAACCCAGA GGCAGGACCA GAGGAAAGAG ACGAGCAGGT GACGGACAAG
    CTCCCTCTAG ACGTCTTCAA TAACTACTTC AGCCTTGGAT TTGATGCTCA TGTGACTTTA
    GAGTTCCACG AATCTCGAGA GGCTAATCCT GAGAAATTTA ACAGCCGGTT CAGAAACAAA
    ATGTTCTATG CAGGGACAGC GTTTTCTGAT TTCTTGATGG GCAGCTCCAA AGACCTGGCT
    AAGCACATCA AAGTAGTCTG TGACGGCACA GATTTAACCT CAAAGGTCCA GGATTTGAAG
    CTGCAATGCC TGGTCTTCCT AAATATCCCC AGGTACTGTG CGGGCACAAT GCCATGGGGC
    AATCCGAGTG AGCATCATGA CTTTGAGCCT CAGCGTCATG ATGATGGCTG CATTGAGGTT
    ATTGGATTCA CAATGACATC ACTGGCCACA CTGCAGGTCG GAGGTCACGG TGAGAGGTTA
    AATCAGTGTC GCGAAGTCAC CCTCACCACC TTTAAGTCAA TCCCCATGCA GGTGGACGGG
    GAGCCTTGTA AACTAGCACC ATCTGTTATT CACATTTCTT TGCGAAACCA GGCCAACATG
    GTGCAGAAAA CCAAGAGACG TACATCTATA CCACTGCTAA ACGATCAGCA ACCGTTTCCA
    GAACGACTAC GGATCAGAGT GAGCCGTATC AGCATGCATG ATTATGAAGC TTTGCACTAC
    GACAAGGAGA AGCTCAAAGA AGCTTCGATT CCACTGGGTC TCATCGTTGT CCCTGGAGAC
    AGTGATTTGG AAACCTGCCG AGCTCACATC GAGAGACTAC AGGAGGAAGG TGACGGGAGC
    AAATCAAAGG CTTTGTCGTC TCAGAGGCTA TCACCGAAAT GGTGCTTTCT CGACTCTACA
    ACTGCTGATC GATTCTACAG GATTGATCGA GCTCAAGAGC ATCTCAACTA CGTGACTGAA
    ATTTCTCAGG ATGAGCTGTT TGTTTTGGAC CCAGAGCTGG TGGTCACAGA GACTGTTAGC
    ACATCACCTG GCATGCCAGA CCTGGTGGAC TCTGGTGAAG TCCAGTCCGA TCCACACAAA
    TTTGCCTTTC CGTCATCCTC TTCTTCACCA CCAAACACAC CTGGACCTAG GGTTGCAGAG
    CTGCAAAGGA AGCGTGTTTC CAGTGACAGT TCGGTGGCAG AAGCACTTGC ACACAGTGAC
    TCGCTCAGAT CAGCCAAGCC AGCTCTCTGC AGAGCTGGGG GTGTTCATCG CTCAAATACA
    ACCGCAGCAG ATTTCAAACC TACCAGCAGG TTTGAGAAGA GCGCTGTGAA CAATGCCAAC
    TCTGTGTCAG TGGACAGTCT AATTGAATGT GTGAAAAAGA AGGACCATCA GAAGTTGAAG
    GAGCTGCATA AGAAAGGGGC CGACCTGTGT GTGCAGGACC CTGCTGGACG GACCCTATTG
    CACTATGCTG TGGAAGTGGG CAGCAAGGAG ATTGTCAAAT ACATCATTGA TAATGCACCC
    ACCGATATCC TGGATGTAAC AGAGAAAGAA AATGGAGAGA CTGTGTTGCA CAAAGCAGCC
    TCGCTGTGTC ATAGGACAAT CTGTCATTAC CTAGTGGAAG CAGGAGCATC TCTTATGAAG
    ACAGACTTAC AGGGTGACAC TCCGAAGCAT CGTGCCGAGA AGGCGAAAGA TGCTGAACTT
    GCGGCATATC TTGAAAACCG CCAGCACTAC CAAATGATCC AGCGTGAGGA CCAGGAGACT
    GCAGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa13282
    Clone ID Related Accession (Same CDS sequence) XM_005169034.3 , XM_005169034.4
    Accession Version XM_005169034.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3669bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product diacylglycerol kinase zeta isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005169034.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
    CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT
    TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG
    CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA
    GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT
    CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC
    CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC
    CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA
    TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA
    CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC
    CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT
    GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG
    ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC
    AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC
    CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA
    ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG
    ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG
    CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC
    ATTGAACAGC TGGAGAAGAT AAACTTCAGA TGTAAACCGT CTTTCAGGGA GTCAGGATCA
    CGGAACATCC GAGAGCCTGC AATAGTGCGA CACCACTGGG TCCATCGGAG AAGACAAGAG
    GGCAAATGCA AACAGTGTGG AAAGGGGTTT CAGCAGAAAT TTGCCTTTCA CAGTAAAGAG
    ATTGTAGCCA TCAGCTGCTC CTGGTGCAAA CAAGCATATC ATAATAAGGT GACATGCTTT
    ATGCTGCAGC AGATAGAAGA ACCATGTTCA TTAGGAGCTC ATGCCGCTGT AATAGTACCT
    CCTACTTGGA TCATACGGGT TCGCCGACCT CAGTCCTCAC TAAAGTCAAG CAAAAAGAAA
    AAACGGACAT CTTTCAAACG TAAATCCAGC AAGAAAGGAG CAGAGGAGGC ACGATGGAAG
    CCTTTTATAG TGAGACCCAT TCCCTCCCAA CTCATGAAGC CACTGCTAGT GTTTGTAAAT
    CCAAAGAGTG GAGGAAACCA GGGAGCTAAA ATCATCCAGA CCTTTCTTTG GTATCTAAAC
    CCTCGGCAGG TGTTTGATCT AAGTCAAGGA GGTCCTCAGG AAGGCTTGGA AATGTATCGC
    AAGGTTCACA ACTTGAGAAT TTTGGCCTGT GGGGGGGATG GCACGGTGGG CTGGATCTTA
    TCTGCACTTG ATCAGCTGCA ATTAAACCCT TCTCCTGCTG TGGCCGTACT TCCCCTGGGC
    ACAGGAAATG ACCTTGCTAG GACGCTAAAC TGGGGTGGGG GCTACACAGA TGAACCTCTG
    AGTAAAATCC TGTCTCATGT GGAGGATGGG AATATTGTAC AGCTGGATCG GTGGAATCTT
    GTTGTGAAGC CCAACCCAGA GGCAGGACCA GAGGAAAGAG ACGAGCAGGT GACGGACAAG
    CTCCCTCTAG ACGTCTTCAA TAACTACTTC AGCCTTGGAT TTGATGCTCA TGTGACTTTA
    GAGTTCCACG AATCTCGAGA GGCTAATCCT GAGAAATTTA ACAGCCGGTT CAGAAACAAA
    ATGTTCTATG CAGGGACAGC GTTTTCTGAT TTCTTGATGG GCAGCTCCAA AGACCTGGCT
    AAGCACATCA AAGTAGTCTG TGACGGCACA GATTTAACCT CAAAGGTCCA GGATTTGAAG
    CTGCAATGCC TGGTCTTCCT AAATATCCCC AGGTACTGTG CGGGCACAAT GCCATGGGGC
    AATCCGAGTG AGCATCATGA CTTTGAGCCT CAGCGTCATG ATGATGGCTG CATTGAGGTT
    ATTGGATTCA CAATGACATC ACTGGCCACA CTGCAGGTCG GAGGTCACGG TGAGAGGTTA
    AATCAGTGTC GCGAAGTCAC CCTCACCACC TTTAAGTCAA TCCCCATGCA GGTGGACGGG
    GAGCCTTGTA AACTAGCACC ATCTGTTATT CACATTTCTT TGCGAAACCA GGCCAACATG
    GTGCAGAAAA CCAAGAGACG TACATCTATA CCACTGCTAA ACGATCAGCA ACCGTTTCCA
    GAACGACTAC GGATCAGAGT GAGCCGTATC AGCATGCATG ATTATGAAGC TTTGCACTAC
    GACAAGGAGA AGCTCAAAGA AGCTTCGATT CCACTGGGTC TCATCGTTGT CCCTGGAGAC
    AGTGATTTGG AAACCTGCCG AGCTCACATC GAGAGACTAC AGGAGGAAGG TGACGGGAGC
    AAATCAAAGG CTTTGTCGTC TCAGAGGCTA TCACCGAAAT GGTGCTTTCT CGACTCTACA
    ACTGCTGATC GATTCTACAG GATTGATCGA GCTCAAGAGC ATCTCAACTA CGTGACTGAA
    ATTTCTCAGG ATGAGCTGTT TGTTTTGGAC CCAGAGCTGG TGGTCACAGA GACTGTTAGC
    ACATCACCTG GCATGCCAGA CCTGGTGGAC TCTGGTGAAG TCCAGTCCGA TCCACACAAA
    TTTGCCTTTC CGTCATCCTC TTCTTCACCA CCAAACACAC CTGGACCTAG GGTTGCAGAG
    CTGCAAAGGA AGCGTGTTTC CAGTGACAGT TCGGTGGCAG AAGCACTTGC ACACAGTGAC
    TCGCTCAGAT CAGCCAAGCC AGCTCTCTGC AGAGCTGGGG GTGTTCATCG CTCAAATACA
    ACCGCAGCAG ATTTCAAACC TACCAGCAGG TTTGAGAAGA GCGCTGTGAA CAATGCCAAC
    TCTGTGTCAG TGGACAGTCT AATTGAATGT GTGAAAAAGA AGGACCATCA GAAGTTGAAG
    GAGCTGCATA AGAAAGGGGC CGACCTGTGT GTGCAGGACC CTGCTGGACG GACCCTATTG
    CACTATGCTG TGGAAGTGGG CAGCAAGGAG ATTGTCAAAT ACATCATTGA TAATGCACCC
    ACCGATATCC TGGATGTAAC AGAGAAAGAA AATGGAGAGA CTGTGTTGCA CAAAGCAGCC
    TCGCTGTGTC ATAGGACAAT CTGTCATTAC CTAGTGGAAG CAGGAGCATC TCTTATGAAG
    ACAGACTTAC AGGGTGACAC TCCGAAGCAT CGTGCCGAGA AGGCGAAAGA TGCTGAACTT
    GCGGCATATC TTGAAAACCG CCAGCACTAC CAAATGATCC AGCGTGAGGA CCAGGAGACT
    GCAGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005169091.1
    CDS170..3838
    Translation

    Target ORF information:

    RefSeq Version XM_005169034.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005169034.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
    CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT
    TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG
    CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA
    GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT
    CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC
    CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC
    CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA
    TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA
    CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC
    CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT
    GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG
    ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC
    AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC
    CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA
    ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG
    ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG
    CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC
    ATTGAACAGC TGGAGAAGAT AAACTTCAGA TGTAAACCGT CTTTCAGGGA GTCAGGATCA
    CGGAACATCC GAGAGCCTGC AATAGTGCGA CACCACTGGG TCCATCGGAG AAGACAAGAG
    GGCAAATGCA AACAGTGTGG AAAGGGGTTT CAGCAGAAAT TTGCCTTTCA CAGTAAAGAG
    ATTGTAGCCA TCAGCTGCTC CTGGTGCAAA CAAGCATATC ATAATAAGGT GACATGCTTT
    ATGCTGCAGC AGATAGAAGA ACCATGTTCA TTAGGAGCTC ATGCCGCTGT AATAGTACCT
    CCTACTTGGA TCATACGGGT TCGCCGACCT CAGTCCTCAC TAAAGTCAAG CAAAAAGAAA
    AAACGGACAT CTTTCAAACG TAAATCCAGC AAGAAAGGAG CAGAGGAGGC ACGATGGAAG
    CCTTTTATAG TGAGACCCAT TCCCTCCCAA CTCATGAAGC CACTGCTAGT GTTTGTAAAT
    CCAAAGAGTG GAGGAAACCA GGGAGCTAAA ATCATCCAGA CCTTTCTTTG GTATCTAAAC
    CCTCGGCAGG TGTTTGATCT AAGTCAAGGA GGTCCTCAGG AAGGCTTGGA AATGTATCGC
    AAGGTTCACA ACTTGAGAAT TTTGGCCTGT GGGGGGGATG GCACGGTGGG CTGGATCTTA
    TCTGCACTTG ATCAGCTGCA ATTAAACCCT TCTCCTGCTG TGGCCGTACT TCCCCTGGGC
    ACAGGAAATG ACCTTGCTAG GACGCTAAAC TGGGGTGGGG GCTACACAGA TGAACCTCTG
    AGTAAAATCC TGTCTCATGT GGAGGATGGG AATATTGTAC AGCTGGATCG GTGGAATCTT
    GTTGTGAAGC CCAACCCAGA GGCAGGACCA GAGGAAAGAG ACGAGCAGGT GACGGACAAG
    CTCCCTCTAG ACGTCTTCAA TAACTACTTC AGCCTTGGAT TTGATGCTCA TGTGACTTTA
    GAGTTCCACG AATCTCGAGA GGCTAATCCT GAGAAATTTA ACAGCCGGTT CAGAAACAAA
    ATGTTCTATG CAGGGACAGC GTTTTCTGAT TTCTTGATGG GCAGCTCCAA AGACCTGGCT
    AAGCACATCA AAGTAGTCTG TGACGGCACA GATTTAACCT CAAAGGTCCA GGATTTGAAG
    CTGCAATGCC TGGTCTTCCT AAATATCCCC AGGTACTGTG CGGGCACAAT GCCATGGGGC
    AATCCGAGTG AGCATCATGA CTTTGAGCCT CAGCGTCATG ATGATGGCTG CATTGAGGTT
    ATTGGATTCA CAATGACATC ACTGGCCACA CTGCAGGTCG GAGGTCACGG TGAGAGGTTA
    AATCAGTGTC GCGAAGTCAC CCTCACCACC TTTAAGTCAA TCCCCATGCA GGTGGACGGG
    GAGCCTTGTA AACTAGCACC ATCTGTTATT CACATTTCTT TGCGAAACCA GGCCAACATG
    GTGCAGAAAA CCAAGAGACG TACATCTATA CCACTGCTAA ACGATCAGCA ACCGTTTCCA
    GAACGACTAC GGATCAGAGT GAGCCGTATC AGCATGCATG ATTATGAAGC TTTGCACTAC
    GACAAGGAGA AGCTCAAAGA AGCTTCGATT CCACTGGGTC TCATCGTTGT CCCTGGAGAC
    AGTGATTTGG AAACCTGCCG AGCTCACATC GAGAGACTAC AGGAGGAAGG TGACGGGAGC
    AAATCAAAGG CTTTGTCGTC TCAGAGGCTA TCACCGAAAT GGTGCTTTCT CGACTCTACA
    ACTGCTGATC GATTCTACAG GATTGATCGA GCTCAAGAGC ATCTCAACTA CGTGACTGAA
    ATTTCTCAGG ATGAGCTGTT TGTTTTGGAC CCAGAGCTGG TGGTCACAGA GACTGTTAGC
    ACATCACCTG GCATGCCAGA CCTGGTGGAC TCTGGTGAAG TCCAGTCCGA TCCACACAAA
    TTTGCCTTTC CGTCATCCTC TTCTTCACCA CCAAACACAC CTGGACCTAG GGTTGCAGAG
    CTGCAAAGGA AGCGTGTTTC CAGTGACAGT TCGGTGGCAG AAGCACTTGC ACACAGTGAC
    TCGCTCAGAT CAGCCAAGCC AGCTCTCTGC AGAGCTGGGG GTGTTCATCG CTCAAATACA
    ACCGCAGCAG ATTTCAAACC TACCAGCAGG TTTGAGAAGA GCGCTGTGAA CAATGCCAAC
    TCTGTGTCAG TGGACAGTCT AATTGAATGT GTGAAAAAGA AGGACCATCA GAAGTTGAAG
    GAGCTGCATA AGAAAGGGGC CGACCTGTGT GTGCAGGACC CTGCTGGACG GACCCTATTG
    CACTATGCTG TGGAAGTGGG CAGCAAGGAG ATTGTCAAAT ACATCATTGA TAATGCACCC
    ACCGATATCC TGGATGTAAC AGAGAAAGAA AATGGAGAGA CTGTGTTGCA CAAAGCAGCC
    TCGCTGTGTC ATAGGACAAT CTGTCATTAC CTAGTGGAAG CAGGAGCATC TCTTATGAAG
    ACAGACTTAC AGGGTGACAC TCCGAAGCAT CGTGCCGAGA AGGCGAAAGA TGCTGAACTT
    GCGGCATATC TTGAAAACCG CCAGCACTAC CAAATGATCC AGCGTGAGGA CCAGGAGACT
    GCAGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa13283
    Clone ID Related Accession (Same CDS sequence) XM_005169035.3
    Accession Version XM_005169035.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3264bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product diacylglycerol kinase zeta isoform X9
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169035.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCAGAGA AATTGGACGC GACCGTTGAC GGTTCGCCAC GCGCCGCGCA CAGCTCCGCG 
    GACTCGCCGA CCACCGTCCT GATTCCGCCT GCCCTGGAGG ACGTGGTGGA GGAAGAGGAC
    GTGTTCCTCT CTGAAACCGG GCACAGCGGA GCAACTTCAA CACCGCACGA GTGTCCGGGG
    AACGGGGTTG AGAGACTGGC GGAGGGGAGA GAAGGAGAAG TGCGTCCTGA CAACCAGGCG
    GAGAGCAGCG AGTTCCTGCA CCTCACTATC CGGAAACAGG TGTCATACAG GAAGGCTATT
    GCCAAGTCCG GTCTCCAGCA CCTCTCATGC CAGCCCAGTG CTGCAGTGTT AGCCAAAGGG
    GAAACGGACA GAGAAATACG CAACACAGTA GACTGGAGTG AATCAGCTGT ATACGGAGAG
    CACATCTGGT TTGAGACGAA TGTCTCGGGG GACTTCTGTT ACGTTGGAGA ACAGCACTGT
    TACGCCAAGT CACTGCAAAA GTCTGTTGCT CGGAAAAAGT GTGCGGCTTG CAAGATCGTA
    GTTCACTCTA TCTGCATTGA ACAGCTGGAG AAGTTATTGG ATTGGAATGC TCCACCTCAG
    GGGGAGGGGC TTGATGAGAT GCCTCTCAAA GAGCCGTCAT TCAGAATAAA CTTCAGATGT
    AAACCGTCTT TCAGGGAGTC AGGATCACGG AACATCCGAG AGCCTGCAAT AGTGCGACAC
    CACTGGGTCC ATCGGAGAAG ACAAGAGGGC AAATGCAAAC AGTGTGGAAA GGGGTTTCAG
    CAGAAATTTG CCTTTCACAG TAAAGAGATT GTAGCCATCA GCTGCTCCTG GTGCAAACAA
    GCATATCATA ATAAGGTGAC ATGCTTTATG CTGCAGCAGA TAGAAGAACC ATGTTCATTA
    GGAGCTCATG CCGCTGTAAT AGTACCTCCT ACTTGGATCA TACGGGTTCG CCGACCTCAG
    TCCTCACTAA AGTCAAGCAA AAAGAAAAAA CGGACATCTT TCAAACGTAA ATCCAGCAAG
    AAAGGAGCAG AGGAGGCACG ATGGAAGCCT TTTATAGTGA GACCCATTCC CTCCCAACTC
    ATGAAGCCAC TGCTAGTGTT TGTAAATCCA AAGAGTGGAG GAAACCAGGG AGCTAAAATC
    ATCCAGACCT TTCTTTGGTA TCTAAACCCT CGGCAGGTGT TTGATCTAAG TCAAGGAGGT
    CCTCAGGAAG GCTTGGAAAT GTATCGCAAG GTTCACAACT TGAGAATTTT GGCCTGTGGG
    GGGGATGGCA CGGTGGGCTG GATCTTATCT GCACTTGATC AGCTGCAATT AAACCCTTCT
    CCTGCTGTGG CCGTACTTCC CCTGGGCACA GGAAATGACC TTGCTAGGAC GCTAAACTGG
    GGTGGGGGCT ACACAGATGA ACCTCTGAGT AAAATCCTGT CTCATGTGGA GGATGGGAAT
    ATTGTACAGC TGGATCGGTG GAATCTTGTT GTGAAGCCCA ACCCAGAGGC AGGACCAGAG
    GAAAGAGACG AGCAGGTGAC GGACAAGCTC CCTCTAGACG TCTTCAATAA CTACTTCAGC
    CTTGGATTTG ATGCTCATGT GACTTTAGAG TTCCACGAAT CTCGAGAGGC TAATCCTGAG
    AAATTTAACA GCCGGTTCAG AAACAAAATG TTCTATGCAG GGACAGCGTT TTCTGATTTC
    TTGATGGGCA GCTCCAAAGA CCTGGCTAAG CACATCAAAG TAGTCTGTGA CGGCACAGAT
    TTAACCTCAA AGGTCCAGGA TTTGAAGCTG CAATGCCTGG TCTTCCTAAA TATCCCCAGG
    TACTGTGCGG GCACAATGCC ATGGGGCAAT CCGAGTGAGC ATCATGACTT TGAGCCTCAG
    CGTCATGATG ATGGCTGCAT TGAGGTTATT GGATTCACAA TGACATCACT GGCCACACTG
    CAGGTCGGAG GTCACGGTGA GAGGTTAAAT CAGTGTCGCG AAGTCACCCT CACCACCTTT
    AAGTCAATCC CCATGCAGGT GGACGGGGAG CCTTGTAAAC TAGCACCATC TGTTATTCAC
    ATTTCTTTGC GAAACCAGGC CAACATGGTG CAGAAAACCA AGAGACGTAC ATCTATACCA
    CTGCTAAACG ACAGTCAGCA ACCGTTTCCA GAACGACTAC GGATCAGAGT GAGCCGTATC
    AGCATGCATG ATTATGAAGC TTTGCACTAC GACAAGGAGA AGCTCAAAGA AGCTTCGATT
    CCACTGGGTC TCATCGTTGT CCCTGGAGAC AGTGATTTGG AAACCTGCCG AGCTCACATC
    GAGAGACTAC AGGAGGACTT TGTGTCGTGT AACCCGTCTC TGCAGCGGCT GGTGCTCCAG
    GAAGGTGACG GGAGCAAATC AAAGGCTTTG TCGTCTCAGA GGCTATCACC GAAATGGTGC
    TTTCTCGACT CTACAACTGC TGATCGATTC TACAGGATTG ATCGAGCTCA AGAGCATCTC
    AACTACGTGA CTGAAATTTC TCAGGATGAG CTGTTTGTTT TGGACCCAGA GCTGGTGGTC
    ACAGAGACTG TTAGCACATC ACCTGGCATG CCAGACCTGG TGGACTCTGG TGAAGTCCAG
    TCCGATCCAC ACAAATTTGC CTTTCCGTCA TCCTCTTCTT CACCACCAAA CACACCTGGA
    CCTAGGGTTG CAGAGCTGCA AAGGAAGCGT GTTTCCAGTG ACAGTTCGGT GGCAGAAGCA
    CTTGCACACA GTGACTCGCT CAGATCAGCC AAGCCAGCTC TCTGCAGAGC TGGGGGTGTT
    CATCGCTCAA ATACAACCGC AGCAGATTTC AAACCTACCA GCAGGTTTGA GAAGAGCGCT
    GTGAACAATG CCAACTCTGT GTCAGTGGAC AGTCTAATTG AATGTGTGAA AAAGAAGGAC
    CATCAGAAGT TGAAGGAGCT GCATAAGAAA GGGGCCGACC TGTGTGTGCA GGACCCTGCT
    GGACGGACCC TATTGCACTA TGCTGTGGAA GTGGGCAGCA AGGAGATTGT CAAATACATC
    ATTGATAATG CACCCACCGA TATCCTGGAT GTAACAGAGA AAGAAAATGG AGAGACTGTG
    TTGCACAAAG CAGCCTCGCT GTGTCATAGG ACAATCTGTC ATTACCTAGT GGAAGCAGGA
    GCATCTCTTA TGAAGACAGA CTTACAGGGT GACACTCCGA AGCATCGTGC CGAGAAGGCG
    AAAGATGCTG AACTTGCGGC ATATCTTGAA AACCGCCAGC ACTACCAAAT GATCCAGCGT
    GAGGACCAGG AGACTGCAGT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005169092.1
    CDS236..3499
    Translation

    Target ORF information:

    RefSeq Version XM_005169035.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005169035.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCAGAGA AATTGGACGC GACCGTTGAC GGTTCGCCAC GCGCCGCGCA CAGCTCCGCG 
    GACTCGCCGA CCACCGTCCT GATTCCGCCT GCCCTGGAGG ACGTGGTGGA GGAAGAGGAC
    GTGTTCCTCT CTGAAACCGG GCACAGCGGA GCAACTTCAA CACCGCACGA GTGTCCGGGG
    AACGGGGTTG AGAGACTGGC GGAGGGGAGA GAAGGAGAAG TGCGTCCTGA CAACCAGGCG
    GAGAGCAGCG AGTTCCTGCA CCTCACTATC CGGAAACAGG TGTCATACAG GAAGGCTATT
    GCCAAGTCCG GTCTCCAGCA CCTCTCATGC CAGCCCAGTG CTGCAGTGTT AGCCAAAGGG
    GAAACGGACA GAGAAATACG CAACACAGTA GACTGGAGTG AATCAGCTGT ATACGGAGAG
    CACATCTGGT TTGAGACGAA TGTCTCGGGG GACTTCTGTT ACGTTGGAGA ACAGCACTGT
    TACGCCAAGT CACTGCAAAA GTCTGTTGCT CGGAAAAAGT GTGCGGCTTG CAAGATCGTA
    GTTCACTCTA TCTGCATTGA ACAGCTGGAG AAGTTATTGG ATTGGAATGC TCCACCTCAG
    GGGGAGGGGC TTGATGAGAT GCCTCTCAAA GAGCCGTCAT TCAGAATAAA CTTCAGATGT
    AAACCGTCTT TCAGGGAGTC AGGATCACGG AACATCCGAG AGCCTGCAAT AGTGCGACAC
    CACTGGGTCC ATCGGAGAAG ACAAGAGGGC AAATGCAAAC AGTGTGGAAA GGGGTTTCAG
    CAGAAATTTG CCTTTCACAG TAAAGAGATT GTAGCCATCA GCTGCTCCTG GTGCAAACAA
    GCATATCATA ATAAGGTGAC ATGCTTTATG CTGCAGCAGA TAGAAGAACC ATGTTCATTA
    GGAGCTCATG CCGCTGTAAT AGTACCTCCT ACTTGGATCA TACGGGTTCG CCGACCTCAG
    TCCTCACTAA AGTCAAGCAA AAAGAAAAAA CGGACATCTT TCAAACGTAA ATCCAGCAAG
    AAAGGAGCAG AGGAGGCACG ATGGAAGCCT TTTATAGTGA GACCCATTCC CTCCCAACTC
    ATGAAGCCAC TGCTAGTGTT TGTAAATCCA AAGAGTGGAG GAAACCAGGG AGCTAAAATC
    ATCCAGACCT TTCTTTGGTA TCTAAACCCT CGGCAGGTGT TTGATCTAAG TCAAGGAGGT
    CCTCAGGAAG GCTTGGAAAT GTATCGCAAG GTTCACAACT TGAGAATTTT GGCCTGTGGG
    GGGGATGGCA CGGTGGGCTG GATCTTATCT GCACTTGATC AGCTGCAATT AAACCCTTCT
    CCTGCTGTGG CCGTACTTCC CCTGGGCACA GGAAATGACC TTGCTAGGAC GCTAAACTGG
    GGTGGGGGCT ACACAGATGA ACCTCTGAGT AAAATCCTGT CTCATGTGGA GGATGGGAAT
    ATTGTACAGC TGGATCGGTG GAATCTTGTT GTGAAGCCCA ACCCAGAGGC AGGACCAGAG
    GAAAGAGACG AGCAGGTGAC GGACAAGCTC CCTCTAGACG TCTTCAATAA CTACTTCAGC
    CTTGGATTTG ATGCTCATGT GACTTTAGAG TTCCACGAAT CTCGAGAGGC TAATCCTGAG
    AAATTTAACA GCCGGTTCAG AAACAAAATG TTCTATGCAG GGACAGCGTT TTCTGATTTC
    TTGATGGGCA GCTCCAAAGA CCTGGCTAAG CACATCAAAG TAGTCTGTGA CGGCACAGAT
    TTAACCTCAA AGGTCCAGGA TTTGAAGCTG CAATGCCTGG TCTTCCTAAA TATCCCCAGG
    TACTGTGCGG GCACAATGCC ATGGGGCAAT CCGAGTGAGC ATCATGACTT TGAGCCTCAG
    CGTCATGATG ATGGCTGCAT TGAGGTTATT GGATTCACAA TGACATCACT GGCCACACTG
    CAGGTCGGAG GTCACGGTGA GAGGTTAAAT CAGTGTCGCG AAGTCACCCT CACCACCTTT
    AAGTCAATCC CCATGCAGGT GGACGGGGAG CCTTGTAAAC TAGCACCATC TGTTATTCAC
    ATTTCTTTGC GAAACCAGGC CAACATGGTG CAGAAAACCA AGAGACGTAC ATCTATACCA
    CTGCTAAACG ACAGTCAGCA ACCGTTTCCA GAACGACTAC GGATCAGAGT GAGCCGTATC
    AGCATGCATG ATTATGAAGC TTTGCACTAC GACAAGGAGA AGCTCAAAGA AGCTTCGATT
    CCACTGGGTC TCATCGTTGT CCCTGGAGAC AGTGATTTGG AAACCTGCCG AGCTCACATC
    GAGAGACTAC AGGAGGACTT TGTGTCGTGT AACCCGTCTC TGCAGCGGCT GGTGCTCCAG
    GAAGGTGACG GGAGCAAATC AAAGGCTTTG TCGTCTCAGA GGCTATCACC GAAATGGTGC
    TTTCTCGACT CTACAACTGC TGATCGATTC TACAGGATTG ATCGAGCTCA AGAGCATCTC
    AACTACGTGA CTGAAATTTC TCAGGATGAG CTGTTTGTTT TGGACCCAGA GCTGGTGGTC
    ACAGAGACTG TTAGCACATC ACCTGGCATG CCAGACCTGG TGGACTCTGG TGAAGTCCAG
    TCCGATCCAC ACAAATTTGC CTTTCCGTCA TCCTCTTCTT CACCACCAAA CACACCTGGA
    CCTAGGGTTG CAGAGCTGCA AAGGAAGCGT GTTTCCAGTG ACAGTTCGGT GGCAGAAGCA
    CTTGCACACA GTGACTCGCT CAGATCAGCC AAGCCAGCTC TCTGCAGAGC TGGGGGTGTT
    CATCGCTCAA ATACAACCGC AGCAGATTTC AAACCTACCA GCAGGTTTGA GAAGAGCGCT
    GTGAACAATG CCAACTCTGT GTCAGTGGAC AGTCTAATTG AATGTGTGAA AAAGAAGGAC
    CATCAGAAGT TGAAGGAGCT GCATAAGAAA GGGGCCGACC TGTGTGTGCA GGACCCTGCT
    GGACGGACCC TATTGCACTA TGCTGTGGAA GTGGGCAGCA AGGAGATTGT CAAATACATC
    ATTGATAATG CACCCACCGA TATCCTGGAT GTAACAGAGA AAGAAAATGG AGAGACTGTG
    TTGCACAAAG CAGCCTCGCT GTGTCATAGG ACAATCTGTC ATTACCTAGT GGAAGCAGGA
    GCATCTCTTA TGAAGACAGA CTTACAGGGT GACACTCCGA AGCATCGTGC CGAGAAGGCG
    AAAGATGCTG AACTTGCGGC ATATCTTGAA AACCGCCAGC ACTACCAAAT GATCCAGCGT
    GAGGACCAGG AGACTGCAGT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa13284
    Clone ID Related Accession (Same CDS sequence) XM_001922410.6 , XM_001922410.5
    Accession Version XM_001922410.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3192bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product diacylglycerol kinase zeta isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001922410.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCAGAGA AATTGGACGC GACCGTTGAC GGTTCGCCAC GCGCCGCGCA CAGCTCCGCG 
    GACTCGCCGA CCACCGTCCT GATTCCGCCT GCCCTGGAGG ACGTGGTGGA GGAAGAGGAC
    GTGTTCCTCT CTGAAACCGG GCACAGCGGA GCAACTTCAA CACCGCACGA GTGTCCGGGG
    AACGGGGTTG AGAGACTGGC GGAGGGGAGA GAAGGAGAAG TGCGTCCTGA CAACCAGGCG
    GAGAGCAGCG AGTTCCTGCA CCTCACTATC CGGAAACAGG TGTCATACAG GAAGGCTATT
    GCCAAGTCCG GTCTCCAGCA CCTCTCATGC CAGCCCAGTG CTGCAGTGTT AGCCAAAGGG
    GAAACGGACA GAGAAATACG CAACACAGTA GACTGGAGTG AATCAGCTGT ATACGGAGAG
    CACATCTGGT TTGAGACGAA TGTCTCGGGG GACTTCTGTT ACGTTGGAGA ACAGCACTGT
    TACGCCAAGT CACTGCAAAA GTCTGTTGCT CGGAAAAAGT GTGCGGCTTG CAAGATCGTA
    GTTCACTCTA TCTGCATTGA ACAGCTGGAG AAGATAAACT TCAGATGTAA ACCGTCTTTC
    AGGGAGTCAG GATCACGGAA CATCCGAGAG CCTGCAATAG TGCGACACCA CTGGGTCCAT
    CGGAGAAGAC AAGAGGGCAA ATGCAAACAG TGTGGAAAGG GGTTTCAGCA GAAATTTGCC
    TTTCACAGTA AAGAGATTGT AGCCATCAGC TGCTCCTGGT GCAAACAAGC ATATCATAAT
    AAGGTGACAT GCTTTATGCT GCAGCAGATA GAAGAACCAT GTTCATTAGG AGCTCATGCC
    GCTGTAATAG TACCTCCTAC TTGGATCATA CGGGTTCGCC GACCTCAGTC CTCACTAAAG
    TCAAGCAAAA AGAAAAAACG GACATCTTTC AAACGTAAAT CCAGCAAGAA AGGAGCAGAG
    GAGGCACGAT GGAAGCCTTT TATAGTGAGA CCCATTCCCT CCCAACTCAT GAAGCCACTG
    CTAGTGTTTG TAAATCCAAA GAGTGGAGGA AACCAGGGAG CTAAAATCAT CCAGACCTTT
    CTTTGGTATC TAAACCCTCG GCAGGTGTTT GATCTAAGTC AAGGAGGTCC TCAGGAAGGC
    TTGGAAATGT ATCGCAAGGT TCACAACTTG AGAATTTTGG CCTGTGGGGG GGATGGCACG
    GTGGGCTGGA TCTTATCTGC ACTTGATCAG CTGCAATTAA ACCCTTCTCC TGCTGTGGCC
    GTACTTCCCC TGGGCACAGG AAATGACCTT GCTAGGACGC TAAACTGGGG TGGGGGCTAC
    ACAGATGAAC CTCTGAGTAA AATCCTGTCT CATGTGGAGG ATGGGAATAT TGTACAGCTG
    GATCGGTGGA ATCTTGTTGT GAAGCCCAAC CCAGAGGCAG GACCAGAGGA AAGAGACGAG
    CAGGTGACGG ACAAGCTCCC TCTAGACGTC TTCAATAACT ACTTCAGCCT TGGATTTGAT
    GCTCATGTGA CTTTAGAGTT CCACGAATCT CGAGAGGCTA ATCCTGAGAA ATTTAACAGC
    CGGTTCAGAA ACAAAATGTT CTATGCAGGG ACAGCGTTTT CTGATTTCTT GATGGGCAGC
    TCCAAAGACC TGGCTAAGCA CATCAAAGTA GTCTGTGACG GCACAGATTT AACCTCAAAG
    GTCCAGGATT TGAAGCTGCA ATGCCTGGTC TTCCTAAATA TCCCCAGGTA CTGTGCGGGC
    ACAATGCCAT GGGGCAATCC GAGTGAGCAT CATGACTTTG AGCCTCAGCG TCATGATGAT
    GGCTGCATTG AGGTTATTGG ATTCACAATG ACATCACTGG CCACACTGCA GGTCGGAGGT
    CACGGTGAGA GGTTAAATCA GTGTCGCGAA GTCACCCTCA CCACCTTTAA GTCAATCCCC
    ATGCAGGTGG ACGGGGAGCC TTGTAAACTA GCACCATCTG TTATTCACAT TTCTTTGCGA
    AACCAGGCCA ACATGGTGCA GAAAACCAAG AGACGTACAT CTATACCACT GCTAAACGAC
    AGTCAGCAAC CGTTTCCAGA ACGACTACGG ATCAGAGTGA GCCGTATCAG CATGCATGAT
    TATGAAGCTT TGCACTACGA CAAGGAGAAG CTCAAAGAAG CTTCGATTCC ACTGGGTCTC
    ATCGTTGTCC CTGGAGACAG TGATTTGGAA ACCTGCCGAG CTCACATCGA GAGACTACAG
    GAGGACTTTG TGTCGTGTAA CCCGTCTCTG CAGCGGCTGG TGCTCCAGGA AGGTGACGGG
    AGCAAATCAA AGGCTTTGTC GTCTCAGAGG CTATCACCGA AATGGTGCTT TCTCGACTCT
    ACAACTGCTG ATCGATTCTA CAGGATTGAT CGAGCTCAAG AGCATCTCAA CTACGTGACT
    GAAATTTCTC AGGATGAGCT GTTTGTTTTG GACCCAGAGC TGGTGGTCAC AGAGACTGTT
    AGCACATCAC CTGGCATGCC AGACCTGGTG GACTCTGGTG AAGTCCAGTC CGATCCACAC
    AAATTTGCCT TTCCGTCATC CTCTTCTTCA CCACCAAACA CACCTGGACC TAGGGTTGCA
    GAGCTGCAAA GGAAGCGTGT TTCCAGTGAC AGTTCGGTGG CAGAAGCACT TGCACACAGT
    GACTCGCTCA GATCAGCCAA GCCAGCTCTC TGCAGAGCTG GGGGTGTTCA TCGCTCAAAT
    ACAACCGCAG CAGATTTCAA ACCTACCAGC AGGTTTGAGA AGAGCGCTGT GAACAATGCC
    AACTCTGTGT CAGTGGACAG TCTAATTGAA TGTGTGAAAA AGAAGGACCA TCAGAAGTTG
    AAGGAGCTGC ATAAGAAAGG GGCCGACCTG TGTGTGCAGG ACCCTGCTGG ACGGACCCTA
    TTGCACTATG CTGTGGAAGT GGGCAGCAAG GAGATTGTCA AATACATCAT TGATAATGCA
    CCCACCGATA TCCTGGATGT AACAGAGAAA GAAAATGGAG AGACTGTGTT GCACAAAGCA
    GCCTCGCTGT GTCATAGGAC AATCTGTCAT TACCTAGTGG AAGCAGGAGC ATCTCTTATG
    AAGACAGACT TACAGGGTGA CACTCCGAAG CATCGTGCCG AGAAGGCGAA AGATGCTGAA
    CTTGCGGCAT ATCTTGAAAA CCGCCAGCAC TACCAAATGA TCCAGCGTGA GGACCAGGAG
    ACTGCAGTTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001922445.3
    CDS236..3427
    Translation

    Target ORF information:

    RefSeq Version XM_001922410.6
    Organism Danio rerio(zebrafish)
    Definition Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001922410.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCAGAGA AATTGGACGC GACCGTTGAC GGTTCGCCAC GCGCCGCGCA CAGCTCCGCG 
    GACTCGCCGA CCACCGTCCT GATTCCGCCT GCCCTGGAGG ACGTGGTGGA GGAAGAGGAC
    GTGTTCCTCT CTGAAACCGG GCACAGCGGA GCAACTTCAA CACCGCACGA GTGTCCGGGG
    AACGGGGTTG AGAGACTGGC GGAGGGGAGA GAAGGAGAAG TGCGTCCTGA CAACCAGGCG
    GAGAGCAGCG AGTTCCTGCA CCTCACTATC CGGAAACAGG TGTCATACAG GAAGGCTATT
    GCCAAGTCCG GTCTCCAGCA CCTCTCATGC CAGCCCAGTG CTGCAGTGTT AGCCAAAGGG
    GAAACGGACA GAGAAATACG CAACACAGTA GACTGGAGTG AATCAGCTGT ATACGGAGAG
    CACATCTGGT TTGAGACGAA TGTCTCGGGG GACTTCTGTT ACGTTGGAGA ACAGCACTGT
    TACGCCAAGT CACTGCAAAA GTCTGTTGCT CGGAAAAAGT GTGCGGCTTG CAAGATCGTA
    GTTCACTCTA TCTGCATTGA ACAGCTGGAG AAGATAAACT TCAGATGTAA ACCGTCTTTC
    AGGGAGTCAG GATCACGGAA CATCCGAGAG CCTGCAATAG TGCGACACCA CTGGGTCCAT
    CGGAGAAGAC AAGAGGGCAA ATGCAAACAG TGTGGAAAGG GGTTTCAGCA GAAATTTGCC
    TTTCACAGTA AAGAGATTGT AGCCATCAGC TGCTCCTGGT GCAAACAAGC ATATCATAAT
    AAGGTGACAT GCTTTATGCT GCAGCAGATA GAAGAACCAT GTTCATTAGG AGCTCATGCC
    GCTGTAATAG TACCTCCTAC TTGGATCATA CGGGTTCGCC GACCTCAGTC CTCACTAAAG
    TCAAGCAAAA AGAAAAAACG GACATCTTTC AAACGTAAAT CCAGCAAGAA AGGAGCAGAG
    GAGGCACGAT GGAAGCCTTT TATAGTGAGA CCCATTCCCT CCCAACTCAT GAAGCCACTG
    CTAGTGTTTG TAAATCCAAA GAGTGGAGGA AACCAGGGAG CTAAAATCAT CCAGACCTTT
    CTTTGGTATC TAAACCCTCG GCAGGTGTTT GATCTAAGTC AAGGAGGTCC TCAGGAAGGC
    TTGGAAATGT ATCGCAAGGT TCACAACTTG AGAATTTTGG CCTGTGGGGG GGATGGCACG
    GTGGGCTGGA TCTTATCTGC ACTTGATCAG CTGCAATTAA ACCCTTCTCC TGCTGTGGCC
    GTACTTCCCC TGGGCACAGG AAATGACCTT GCTAGGACGC TAAACTGGGG TGGGGGCTAC
    ACAGATGAAC CTCTGAGTAA AATCCTGTCT CATGTGGAGG ATGGGAATAT TGTACAGCTG
    GATCGGTGGA ATCTTGTTGT GAAGCCCAAC CCAGAGGCAG GACCAGAGGA AAGAGACGAG
    CAGGTGACGG ACAAGCTCCC TCTAGACGTC TTCAATAACT ACTTCAGCCT TGGATTTGAT
    GCTCATGTGA CTTTAGAGTT CCACGAATCT CGAGAGGCTA ATCCTGAGAA ATTTAACAGC
    CGGTTCAGAA ACAAAATGTT CTATGCAGGG ACAGCGTTTT CTGATTTCTT GATGGGCAGC
    TCCAAAGACC TGGCTAAGCA CATCAAAGTA GTCTGTGACG GCACAGATTT AACCTCAAAG
    GTCCAGGATT TGAAGCTGCA ATGCCTGGTC TTCCTAAATA TCCCCAGGTA CTGTGCGGGC
    ACAATGCCAT GGGGCAATCC GAGTGAGCAT CATGACTTTG AGCCTCAGCG TCATGATGAT
    GGCTGCATTG AGGTTATTGG ATTCACAATG ACATCACTGG CCACACTGCA GGTCGGAGGT
    CACGGTGAGA GGTTAAATCA GTGTCGCGAA GTCACCCTCA CCACCTTTAA GTCAATCCCC
    ATGCAGGTGG ACGGGGAGCC TTGTAAACTA GCACCATCTG TTATTCACAT TTCTTTGCGA
    AACCAGGCCA ACATGGTGCA GAAAACCAAG AGACGTACAT CTATACCACT GCTAAACGAC
    AGTCAGCAAC CGTTTCCAGA ACGACTACGG ATCAGAGTGA GCCGTATCAG CATGCATGAT
    TATGAAGCTT TGCACTACGA CAAGGAGAAG CTCAAAGAAG CTTCGATTCC ACTGGGTCTC
    ATCGTTGTCC CTGGAGACAG TGATTTGGAA ACCTGCCGAG CTCACATCGA GAGACTACAG
    GAGGACTTTG TGTCGTGTAA CCCGTCTCTG CAGCGGCTGG TGCTCCAGGA AGGTGACGGG
    AGCAAATCAA AGGCTTTGTC GTCTCAGAGG CTATCACCGA AATGGTGCTT TCTCGACTCT
    ACAACTGCTG ATCGATTCTA CAGGATTGAT CGAGCTCAAG AGCATCTCAA CTACGTGACT
    GAAATTTCTC AGGATGAGCT GTTTGTTTTG GACCCAGAGC TGGTGGTCAC AGAGACTGTT
    AGCACATCAC CTGGCATGCC AGACCTGGTG GACTCTGGTG AAGTCCAGTC CGATCCACAC
    AAATTTGCCT TTCCGTCATC CTCTTCTTCA CCACCAAACA CACCTGGACC TAGGGTTGCA
    GAGCTGCAAA GGAAGCGTGT TTCCAGTGAC AGTTCGGTGG CAGAAGCACT TGCACACAGT
    GACTCGCTCA GATCAGCCAA GCCAGCTCTC TGCAGAGCTG GGGGTGTTCA TCGCTCAAAT
    ACAACCGCAG CAGATTTCAA ACCTACCAGC AGGTTTGAGA AGAGCGCTGT GAACAATGCC
    AACTCTGTGT CAGTGGACAG TCTAATTGAA TGTGTGAAAA AGAAGGACCA TCAGAAGTTG
    AAGGAGCTGC ATAAGAAAGG GGCCGACCTG TGTGTGCAGG ACCCTGCTGG ACGGACCCTA
    TTGCACTATG CTGTGGAAGT GGGCAGCAAG GAGATTGTCA AATACATCAT TGATAATGCA
    CCCACCGATA TCCTGGATGT AACAGAGAAA GAAAATGGAG AGACTGTGTT GCACAAAGCA
    GCCTCGCTGT GTCATAGGAC AATCTGTCAT TACCTAGTGG AAGCAGGAGC ATCTCTTATG
    AAGACAGACT TACAGGGTGA CACTCCGAAG CATCGTGCCG AGAAGGCGAA AGATGCTGAA
    CTTGCGGCAT ATCTTGAAAA CCGCCAGCAC TACCAAATGA TCCAGCGTGA GGACCAGGAG
    ACTGCAGTTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa13284
    Clone ID Related Accession (Same CDS sequence) XM_001922410.6 , XM_001922410.5
    Accession Version XM_001922410.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3192bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product diacylglycerol kinase zeta isoform X10
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001922410.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCAGAGA AATTGGACGC GACCGTTGAC GGTTCGCCAC GCGCCGCGCA CAGCTCCGCG 
    GACTCGCCGA CCACCGTCCT GATTCCGCCT GCCCTGGAGG ACGTGGTGGA GGAAGAGGAC
    GTGTTCCTCT CTGAAACCGG GCACAGCGGA GCAACTTCAA CACCGCACGA GTGTCCGGGG
    AACGGGGTTG AGAGACTGGC GGAGGGGAGA GAAGGAGAAG TGCGTCCTGA CAACCAGGCG
    GAGAGCAGCG AGTTCCTGCA CCTCACTATC CGGAAACAGG TGTCATACAG GAAGGCTATT
    GCCAAGTCCG GTCTCCAGCA CCTCTCATGC CAGCCCAGTG CTGCAGTGTT AGCCAAAGGG
    GAAACGGACA GAGAAATACG CAACACAGTA GACTGGAGTG AATCAGCTGT ATACGGAGAG
    CACATCTGGT TTGAGACGAA TGTCTCGGGG GACTTCTGTT ACGTTGGAGA ACAGCACTGT
    TACGCCAAGT CACTGCAAAA GTCTGTTGCT CGGAAAAAGT GTGCGGCTTG CAAGATCGTA
    GTTCACTCTA TCTGCATTGA ACAGCTGGAG AAGATAAACT TCAGATGTAA ACCGTCTTTC
    AGGGAGTCAG GATCACGGAA CATCCGAGAG CCTGCAATAG TGCGACACCA CTGGGTCCAT
    CGGAGAAGAC AAGAGGGCAA ATGCAAACAG TGTGGAAAGG GGTTTCAGCA GAAATTTGCC
    TTTCACAGTA AAGAGATTGT AGCCATCAGC TGCTCCTGGT GCAAACAAGC ATATCATAAT
    AAGGTGACAT GCTTTATGCT GCAGCAGATA GAAGAACCAT GTTCATTAGG AGCTCATGCC
    GCTGTAATAG TACCTCCTAC TTGGATCATA CGGGTTCGCC GACCTCAGTC CTCACTAAAG
    TCAAGCAAAA AGAAAAAACG GACATCTTTC AAACGTAAAT CCAGCAAGAA AGGAGCAGAG
    GAGGCACGAT GGAAGCCTTT TATAGTGAGA CCCATTCCCT CCCAACTCAT GAAGCCACTG
    CTAGTGTTTG TAAATCCAAA GAGTGGAGGA AACCAGGGAG CTAAAATCAT CCAGACCTTT
    CTTTGGTATC TAAACCCTCG GCAGGTGTTT GATCTAAGTC AAGGAGGTCC TCAGGAAGGC
    TTGGAAATGT ATCGCAAGGT TCACAACTTG AGAATTTTGG CCTGTGGGGG GGATGGCACG
    GTGGGCTGGA TCTTATCTGC ACTTGATCAG CTGCAATTAA ACCCTTCTCC TGCTGTGGCC
    GTACTTCCCC TGGGCACAGG AAATGACCTT GCTAGGACGC TAAACTGGGG TGGGGGCTAC
    ACAGATGAAC CTCTGAGTAA AATCCTGTCT CATGTGGAGG ATGGGAATAT TGTACAGCTG
    GATCGGTGGA ATCTTGTTGT GAAGCCCAAC CCAGAGGCAG GACCAGAGGA AAGAGACGAG
    CAGGTGACGG ACAAGCTCCC TCTAGACGTC TTCAATAACT ACTTCAGCCT TGGATTTGAT
    GCTCATGTGA CTTTAGAGTT CCACGAATCT CGAGAGGCTA ATCCTGAGAA ATTTAACAGC
    CGGTTCAGAA ACAAAATGTT CTATGCAGGG ACAGCGTTTT CTGATTTCTT GATGGGCAGC
    TCCAAAGACC TGGCTAAGCA CATCAAAGTA GTCTGTGACG GCACAGATTT AACCTCAAAG
    GTCCAGGATT TGAAGCTGCA ATGCCTGGTC TTCCTAAATA TCCCCAGGTA CTGTGCGGGC
    ACAATGCCAT GGGGCAATCC GAGTGAGCAT CATGACTTTG AGCCTCAGCG TCATGATGAT
    GGCTGCATTG AGGTTATTGG ATTCACAATG ACATCACTGG CCACACTGCA GGTCGGAGGT
    CACGGTGAGA GGTTAAATCA GTGTCGCGAA GTCACCCTCA CCACCTTTAA GTCAATCCCC
    ATGCAGGTGG ACGGGGAGCC TTGTAAACTA GCACCATCTG TTATTCACAT TTCTTTGCGA
    AACCAGGCCA ACATGGTGCA GAAAACCAAG AGACGTACAT CTATACCACT GCTAAACGAC
    AGTCAGCAAC CGTTTCCAGA ACGACTACGG ATCAGAGTGA GCCGTATCAG CATGCATGAT
    TATGAAGCTT TGCACTACGA CAAGGAGAAG CTCAAAGAAG CTTCGATTCC ACTGGGTCTC
    ATCGTTGTCC CTGGAGACAG TGATTTGGAA ACCTGCCGAG CTCACATCGA GAGACTACAG
    GAGGACTTTG TGTCGTGTAA CCCGTCTCTG CAGCGGCTGG TGCTCCAGGA AGGTGACGGG
    AGCAAATCAA AGGCTTTGTC GTCTCAGAGG CTATCACCGA AATGGTGCTT TCTCGACTCT
    ACAACTGCTG ATCGATTCTA CAGGATTGAT CGAGCTCAAG AGCATCTCAA CTACGTGACT
    GAAATTTCTC AGGATGAGCT GTTTGTTTTG GACCCAGAGC TGGTGGTCAC AGAGACTGTT
    AGCACATCAC CTGGCATGCC AGACCTGGTG GACTCTGGTG AAGTCCAGTC CGATCCACAC
    AAATTTGCCT TTCCGTCATC CTCTTCTTCA CCACCAAACA CACCTGGACC TAGGGTTGCA
    GAGCTGCAAA GGAAGCGTGT TTCCAGTGAC AGTTCGGTGG CAGAAGCACT TGCACACAGT
    GACTCGCTCA GATCAGCCAA GCCAGCTCTC TGCAGAGCTG GGGGTGTTCA TCGCTCAAAT
    ACAACCGCAG CAGATTTCAA ACCTACCAGC AGGTTTGAGA AGAGCGCTGT GAACAATGCC
    AACTCTGTGT CAGTGGACAG TCTAATTGAA TGTGTGAAAA AGAAGGACCA TCAGAAGTTG
    AAGGAGCTGC ATAAGAAAGG GGCCGACCTG TGTGTGCAGG ACCCTGCTGG ACGGACCCTA
    TTGCACTATG CTGTGGAAGT GGGCAGCAAG GAGATTGTCA AATACATCAT TGATAATGCA
    CCCACCGATA TCCTGGATGT AACAGAGAAA GAAAATGGAG AGACTGTGTT GCACAAAGCA
    GCCTCGCTGT GTCATAGGAC AATCTGTCAT TACCTAGTGG AAGCAGGAGC ATCTCTTATG
    AAGACAGACT TACAGGGTGA CACTCCGAAG CATCGTGCCG AGAAGGCGAA AGATGCTGAA
    CTTGCGGCAT ATCTTGAAAA CCGCCAGCAC TACCAAATGA TCCAGCGTGA GGACCAGGAG
    ACTGCAGTTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001922445.3
    CDS235..3426
    Translation

    Target ORF information:

    RefSeq Version XM_001922410.5
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001922410.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCAGAGA AATTGGACGC GACCGTTGAC GGTTCGCCAC GCGCCGCGCA CAGCTCCGCG 
    GACTCGCCGA CCACCGTCCT GATTCCGCCT GCCCTGGAGG ACGTGGTGGA GGAAGAGGAC
    GTGTTCCTCT CTGAAACCGG GCACAGCGGA GCAACTTCAA CACCGCACGA GTGTCCGGGG
    AACGGGGTTG AGAGACTGGC GGAGGGGAGA GAAGGAGAAG TGCGTCCTGA CAACCAGGCG
    GAGAGCAGCG AGTTCCTGCA CCTCACTATC CGGAAACAGG TGTCATACAG GAAGGCTATT
    GCCAAGTCCG GTCTCCAGCA CCTCTCATGC CAGCCCAGTG CTGCAGTGTT AGCCAAAGGG
    GAAACGGACA GAGAAATACG CAACACAGTA GACTGGAGTG AATCAGCTGT ATACGGAGAG
    CACATCTGGT TTGAGACGAA TGTCTCGGGG GACTTCTGTT ACGTTGGAGA ACAGCACTGT
    TACGCCAAGT CACTGCAAAA GTCTGTTGCT CGGAAAAAGT GTGCGGCTTG CAAGATCGTA
    GTTCACTCTA TCTGCATTGA ACAGCTGGAG AAGATAAACT TCAGATGTAA ACCGTCTTTC
    AGGGAGTCAG GATCACGGAA CATCCGAGAG CCTGCAATAG TGCGACACCA CTGGGTCCAT
    CGGAGAAGAC AAGAGGGCAA ATGCAAACAG TGTGGAAAGG GGTTTCAGCA GAAATTTGCC
    TTTCACAGTA AAGAGATTGT AGCCATCAGC TGCTCCTGGT GCAAACAAGC ATATCATAAT
    AAGGTGACAT GCTTTATGCT GCAGCAGATA GAAGAACCAT GTTCATTAGG AGCTCATGCC
    GCTGTAATAG TACCTCCTAC TTGGATCATA CGGGTTCGCC GACCTCAGTC CTCACTAAAG
    TCAAGCAAAA AGAAAAAACG GACATCTTTC AAACGTAAAT CCAGCAAGAA AGGAGCAGAG
    GAGGCACGAT GGAAGCCTTT TATAGTGAGA CCCATTCCCT CCCAACTCAT GAAGCCACTG
    CTAGTGTTTG TAAATCCAAA GAGTGGAGGA AACCAGGGAG CTAAAATCAT CCAGACCTTT
    CTTTGGTATC TAAACCCTCG GCAGGTGTTT GATCTAAGTC AAGGAGGTCC TCAGGAAGGC
    TTGGAAATGT ATCGCAAGGT TCACAACTTG AGAATTTTGG CCTGTGGGGG GGATGGCACG
    GTGGGCTGGA TCTTATCTGC ACTTGATCAG CTGCAATTAA ACCCTTCTCC TGCTGTGGCC
    GTACTTCCCC TGGGCACAGG AAATGACCTT GCTAGGACGC TAAACTGGGG TGGGGGCTAC
    ACAGATGAAC CTCTGAGTAA AATCCTGTCT CATGTGGAGG ATGGGAATAT TGTACAGCTG
    GATCGGTGGA ATCTTGTTGT GAAGCCCAAC CCAGAGGCAG GACCAGAGGA AAGAGACGAG
    CAGGTGACGG ACAAGCTCCC TCTAGACGTC TTCAATAACT ACTTCAGCCT TGGATTTGAT
    GCTCATGTGA CTTTAGAGTT CCACGAATCT CGAGAGGCTA ATCCTGAGAA ATTTAACAGC
    CGGTTCAGAA ACAAAATGTT CTATGCAGGG ACAGCGTTTT CTGATTTCTT GATGGGCAGC
    TCCAAAGACC TGGCTAAGCA CATCAAAGTA GTCTGTGACG GCACAGATTT AACCTCAAAG
    GTCCAGGATT TGAAGCTGCA ATGCCTGGTC TTCCTAAATA TCCCCAGGTA CTGTGCGGGC
    ACAATGCCAT GGGGCAATCC GAGTGAGCAT CATGACTTTG AGCCTCAGCG TCATGATGAT
    GGCTGCATTG AGGTTATTGG ATTCACAATG ACATCACTGG CCACACTGCA GGTCGGAGGT
    CACGGTGAGA GGTTAAATCA GTGTCGCGAA GTCACCCTCA CCACCTTTAA GTCAATCCCC
    ATGCAGGTGG ACGGGGAGCC TTGTAAACTA GCACCATCTG TTATTCACAT TTCTTTGCGA
    AACCAGGCCA ACATGGTGCA GAAAACCAAG AGACGTACAT CTATACCACT GCTAAACGAC
    AGTCAGCAAC CGTTTCCAGA ACGACTACGG ATCAGAGTGA GCCGTATCAG CATGCATGAT
    TATGAAGCTT TGCACTACGA CAAGGAGAAG CTCAAAGAAG CTTCGATTCC ACTGGGTCTC
    ATCGTTGTCC CTGGAGACAG TGATTTGGAA ACCTGCCGAG CTCACATCGA GAGACTACAG
    GAGGACTTTG TGTCGTGTAA CCCGTCTCTG CAGCGGCTGG TGCTCCAGGA AGGTGACGGG
    AGCAAATCAA AGGCTTTGTC GTCTCAGAGG CTATCACCGA AATGGTGCTT TCTCGACTCT
    ACAACTGCTG ATCGATTCTA CAGGATTGAT CGAGCTCAAG AGCATCTCAA CTACGTGACT
    GAAATTTCTC AGGATGAGCT GTTTGTTTTG GACCCAGAGC TGGTGGTCAC AGAGACTGTT
    AGCACATCAC CTGGCATGCC AGACCTGGTG GACTCTGGTG AAGTCCAGTC CGATCCACAC
    AAATTTGCCT TTCCGTCATC CTCTTCTTCA CCACCAAACA CACCTGGACC TAGGGTTGCA
    GAGCTGCAAA GGAAGCGTGT TTCCAGTGAC AGTTCGGTGG CAGAAGCACT TGCACACAGT
    GACTCGCTCA GATCAGCCAA GCCAGCTCTC TGCAGAGCTG GGGGTGTTCA TCGCTCAAAT
    ACAACCGCAG CAGATTTCAA ACCTACCAGC AGGTTTGAGA AGAGCGCTGT GAACAATGCC
    AACTCTGTGT CAGTGGACAG TCTAATTGAA TGTGTGAAAA AGAAGGACCA TCAGAAGTTG
    AAGGAGCTGC ATAAGAAAGG GGCCGACCTG TGTGTGCAGG ACCCTGCTGG ACGGACCCTA
    TTGCACTATG CTGTGGAAGT GGGCAGCAAG GAGATTGTCA AATACATCAT TGATAATGCA
    CCCACCGATA TCCTGGATGT AACAGAGAAA GAAAATGGAG AGACTGTGTT GCACAAAGCA
    GCCTCGCTGT GTCATAGGAC AATCTGTCAT TACCTAGTGG AAGCAGGAGC ATCTCTTATG
    AAGACAGACT TACAGGGTGA CACTCCGAAG CATCGTGCCG AGAAGGCGAA AGATGCTGAA
    CTTGCGGCAT ATCTTGAAAA CCGCCAGCAC TACCAAATGA TCCAGCGTGA GGACCAGGAG
    ACTGCAGTTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa13285
    Clone ID Related Accession (Same CDS sequence) XM_005169036.3
    Accession Version XM_005169036.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3159bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product diacylglycerol kinase zeta isoform X11
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169036.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGAGCAAG AGCTGGGAGC AGGTTCTCAG GACAGGGAAG AGGTCACAGC CTCTCTGGCC 
    TCCACGTCCG CTTCTTCATC TAACTCTGAC ATCCCATCCA AGCCTTTGCC CGAGGGGGAT
    AGAGTGGCGG TTAGTCGGCG GCACAGCAGC AGGACTTTTA CTGGGTTACG ACTGTTTGGA
    AGGAGGAAGG CTATTGCCAA GTCCGGTCTC CAGCACCTCT CATGCCAGCC CAGTGCTGCA
    GTGTTAGCCA AAGGGGAAAC GGACAGAGAA ATACGCAACA CAGTAGACTG GAGTGAATCA
    GCTGTATACG GAGAGCACAT CTGGTTTGAG ACGAATGTCT CGGGGGACTT CTGTTACGTT
    GGAGAACAGC ACTGTTACGC CAAGTCACTG CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG
    GCTTGCAAGA TCGTAGTTCA CTCTATCTGC ATTGAACAGC TGGAGAAGTT ATTGGATTGG
    AATGCTCCAC CTCAGGGGGA GGGGCTTGAT GAGATGCCTC TCAAAGAGCC GTCATTCAGA
    ATAAACTTCA GATGTAAACC GTCTTTCAGG GAGTCAGGAT CACGGAACAT CCGAGAGCCT
    GCAATAGTGC GACACCACTG GGTCCATCGG AGAAGACAAG AGGGCAAATG CAAACAGTGT
    GGAAAGGGGT TTCAGCAGAA ATTTGCCTTT CACAGTAAAG AGATTGTAGC CATCAGCTGC
    TCCTGGTGCA AACAAGCATA TCATAATAAG GTGACATGCT TTATGCTGCA GCAGATAGAA
    GAACCATGTT CATTAGGAGC TCATGCCGCT GTAATAGTAC CTCCTACTTG GATCATACGG
    GTTCGCCGAC CTCAGTCCTC ACTAAAGTCA AGCAAAAAGA AAAAACGGAC ATCTTTCAAA
    CGTAAATCCA GCAAGAAAGG AGCAGAGGAG GCACGATGGA AGCCTTTTAT AGTGAGACCC
    ATTCCCTCCC AACTCATGAA GCCACTGCTA GTGTTTGTAA ATCCAAAGAG TGGAGGAAAC
    CAGGGAGCTA AAATCATCCA GACCTTTCTT TGGTATCTAA ACCCTCGGCA GGTGTTTGAT
    CTAAGTCAAG GAGGTCCTCA GGAAGGCTTG GAAATGTATC GCAAGGTTCA CAACTTGAGA
    ATTTTGGCCT GTGGGGGGGA TGGCACGGTG GGCTGGATCT TATCTGCACT TGATCAGCTG
    CAATTAAACC CTTCTCCTGC TGTGGCCGTA CTTCCCCTGG GCACAGGAAA TGACCTTGCT
    AGGACGCTAA ACTGGGGTGG GGGCTACACA GATGAACCTC TGAGTAAAAT CCTGTCTCAT
    GTGGAGGATG GGAATATTGT ACAGCTGGAT CGGTGGAATC TTGTTGTGAA GCCCAACCCA
    GAGGCAGGAC CAGAGGAAAG AGACGAGCAG GTGACGGACA AGCTCCCTCT AGACGTCTTC
    AATAACTACT TCAGCCTTGG ATTTGATGCT CATGTGACTT TAGAGTTCCA CGAATCTCGA
    GAGGCTAATC CTGAGAAATT TAACAGCCGG TTCAGAAACA AAATGTTCTA TGCAGGGACA
    GCGTTTTCTG ATTTCTTGAT GGGCAGCTCC AAAGACCTGG CTAAGCACAT CAAAGTAGTC
    TGTGACGGCA CAGATTTAAC CTCAAAGGTC CAGGATTTGA AGCTGCAATG CCTGGTCTTC
    CTAAATATCC CCAGGTACTG TGCGGGCACA ATGCCATGGG GCAATCCGAG TGAGCATCAT
    GACTTTGAGC CTCAGCGTCA TGATGATGGC TGCATTGAGG TTATTGGATT CACAATGACA
    TCACTGGCCA CACTGCAGGT CGGAGGTCAC GGTGAGAGGT TAAATCAGTG TCGCGAAGTC
    ACCCTCACCA CCTTTAAGTC AATCCCCATG CAGGTGGACG GGGAGCCTTG TAAACTAGCA
    CCATCTGTTA TTCACATTTC TTTGCGAAAC CAGGCCAACA TGGTGCAGAA AACCAAGAGA
    CGTACATCTA TACCACTGCT AAACGACAGT CAGCAACCGT TTCCAGAACG ACTACGGATC
    AGAGTGAGCC GTATCAGCAT GCATGATTAT GAAGCTTTGC ACTACGACAA GGAGAAGCTC
    AAAGAAGCTT CGATTCCACT GGGTCTCATC GTTGTCCCTG GAGACAGTGA TTTGGAAACC
    TGCCGAGCTC ACATCGAGAG ACTACAGGAG GACTTTGTGT CGTGTAACCC GTCTCTGCAG
    CGGCTGGTGC TCCAGGAAGG TGACGGGAGC AAATCAAAGG CTTTGTCGTC TCAGAGGCTA
    TCACCGAAAT GGTGCTTTCT CGACTCTACA ACTGCTGATC GATTCTACAG GATTGATCGA
    GCTCAAGAGC ATCTCAACTA CGTGACTGAA ATTTCTCAGG ATGAGCTGTT TGTTTTGGAC
    CCAGAGCTGG TGGTCACAGA GACTGTTAGC ACATCACCTG GCATGCCAGA CCTGGTGGAC
    TCTGGTGAAG TCCAGTCCGA TCCACACAAA TTTGCCTTTC CGTCATCCTC TTCTTCACCA
    CCAAACACAC CTGGACCTAG GGTTGCAGAG CTGCAAAGGA AGCGTGTTTC CAGTGACAGT
    TCGGTGGCAG AAGCACTTGC ACACAGTGAC TCGCTCAGAT CAGCCAAGCC AGCTCTCTGC
    AGAGCTGGGG GTGTTCATCG CTCAAATACA ACCGCAGCAG ATTTCAAACC TACCAGCAGG
    TTTGAGAAGA GCGCTGTGAA CAATGCCAAC TCTGTGTCAG TGGACAGTCT AATTGAATGT
    GTGAAAAAGA AGGACCATCA GAAGTTGAAG GAGCTGCATA AGAAAGGGGC CGACCTGTGT
    GTGCAGGACC CTGCTGGACG GACCCTATTG CACTATGCTG TGGAAGTGGG CAGCAAGGAG
    ATTGTCAAAT ACATCATTGA TAATGCACCC ACCGATATCC TGGATGTAAC AGAGAAAGAA
    AATGGAGAGA CTGTGTTGCA CAAAGCAGCC TCGCTGTGTC ATAGGACAAT CTGTCATTAC
    CTAGTGGAAG CAGGAGCATC TCTTATGAAG ACAGACTTAC AGGGTGACAC TCCGAAGCAT
    CGTGCCGAGA AGGCGAAAGA TGCTGAACTT GCGGCATATC TTGAAAACCG CCAGCACTAC
    CAAATGATCC AGCGTGAGGA CCAGGAGACT GCAGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005169093.1
    CDS513..3671
    Translation

    Target ORF information:

    RefSeq Version XM_005169036.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005169036.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGAGCAAG AGCTGGGAGC AGGTTCTCAG GACAGGGAAG AGGTCACAGC CTCTCTGGCC 
    TCCACGTCCG CTTCTTCATC TAACTCTGAC ATCCCATCCA AGCCTTTGCC CGAGGGGGAT
    AGAGTGGCGG TTAGTCGGCG GCACAGCAGC AGGACTTTTA CTGGGTTACG ACTGTTTGGA
    AGGAGGAAGG CTATTGCCAA GTCCGGTCTC CAGCACCTCT CATGCCAGCC CAGTGCTGCA
    GTGTTAGCCA AAGGGGAAAC GGACAGAGAA ATACGCAACA CAGTAGACTG GAGTGAATCA
    GCTGTATACG GAGAGCACAT CTGGTTTGAG ACGAATGTCT CGGGGGACTT CTGTTACGTT
    GGAGAACAGC ACTGTTACGC CAAGTCACTG CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG
    GCTTGCAAGA TCGTAGTTCA CTCTATCTGC ATTGAACAGC TGGAGAAGTT ATTGGATTGG
    AATGCTCCAC CTCAGGGGGA GGGGCTTGAT GAGATGCCTC TCAAAGAGCC GTCATTCAGA
    ATAAACTTCA GATGTAAACC GTCTTTCAGG GAGTCAGGAT CACGGAACAT CCGAGAGCCT
    GCAATAGTGC GACACCACTG GGTCCATCGG AGAAGACAAG AGGGCAAATG CAAACAGTGT
    GGAAAGGGGT TTCAGCAGAA ATTTGCCTTT CACAGTAAAG AGATTGTAGC CATCAGCTGC
    TCCTGGTGCA AACAAGCATA TCATAATAAG GTGACATGCT TTATGCTGCA GCAGATAGAA
    GAACCATGTT CATTAGGAGC TCATGCCGCT GTAATAGTAC CTCCTACTTG GATCATACGG
    GTTCGCCGAC CTCAGTCCTC ACTAAAGTCA AGCAAAAAGA AAAAACGGAC ATCTTTCAAA
    CGTAAATCCA GCAAGAAAGG AGCAGAGGAG GCACGATGGA AGCCTTTTAT AGTGAGACCC
    ATTCCCTCCC AACTCATGAA GCCACTGCTA GTGTTTGTAA ATCCAAAGAG TGGAGGAAAC
    CAGGGAGCTA AAATCATCCA GACCTTTCTT TGGTATCTAA ACCCTCGGCA GGTGTTTGAT
    CTAAGTCAAG GAGGTCCTCA GGAAGGCTTG GAAATGTATC GCAAGGTTCA CAACTTGAGA
    ATTTTGGCCT GTGGGGGGGA TGGCACGGTG GGCTGGATCT TATCTGCACT TGATCAGCTG
    CAATTAAACC CTTCTCCTGC TGTGGCCGTA CTTCCCCTGG GCACAGGAAA TGACCTTGCT
    AGGACGCTAA ACTGGGGTGG GGGCTACACA GATGAACCTC TGAGTAAAAT CCTGTCTCAT
    GTGGAGGATG GGAATATTGT ACAGCTGGAT CGGTGGAATC TTGTTGTGAA GCCCAACCCA
    GAGGCAGGAC CAGAGGAAAG AGACGAGCAG GTGACGGACA AGCTCCCTCT AGACGTCTTC
    AATAACTACT TCAGCCTTGG ATTTGATGCT CATGTGACTT TAGAGTTCCA CGAATCTCGA
    GAGGCTAATC CTGAGAAATT TAACAGCCGG TTCAGAAACA AAATGTTCTA TGCAGGGACA
    GCGTTTTCTG ATTTCTTGAT GGGCAGCTCC AAAGACCTGG CTAAGCACAT CAAAGTAGTC
    TGTGACGGCA CAGATTTAAC CTCAAAGGTC CAGGATTTGA AGCTGCAATG CCTGGTCTTC
    CTAAATATCC CCAGGTACTG TGCGGGCACA ATGCCATGGG GCAATCCGAG TGAGCATCAT
    GACTTTGAGC CTCAGCGTCA TGATGATGGC TGCATTGAGG TTATTGGATT CACAATGACA
    TCACTGGCCA CACTGCAGGT CGGAGGTCAC GGTGAGAGGT TAAATCAGTG TCGCGAAGTC
    ACCCTCACCA CCTTTAAGTC AATCCCCATG CAGGTGGACG GGGAGCCTTG TAAACTAGCA
    CCATCTGTTA TTCACATTTC TTTGCGAAAC CAGGCCAACA TGGTGCAGAA AACCAAGAGA
    CGTACATCTA TACCACTGCT AAACGACAGT CAGCAACCGT TTCCAGAACG ACTACGGATC
    AGAGTGAGCC GTATCAGCAT GCATGATTAT GAAGCTTTGC ACTACGACAA GGAGAAGCTC
    AAAGAAGCTT CGATTCCACT GGGTCTCATC GTTGTCCCTG GAGACAGTGA TTTGGAAACC
    TGCCGAGCTC ACATCGAGAG ACTACAGGAG GACTTTGTGT CGTGTAACCC GTCTCTGCAG
    CGGCTGGTGC TCCAGGAAGG TGACGGGAGC AAATCAAAGG CTTTGTCGTC TCAGAGGCTA
    TCACCGAAAT GGTGCTTTCT CGACTCTACA ACTGCTGATC GATTCTACAG GATTGATCGA
    GCTCAAGAGC ATCTCAACTA CGTGACTGAA ATTTCTCAGG ATGAGCTGTT TGTTTTGGAC
    CCAGAGCTGG TGGTCACAGA GACTGTTAGC ACATCACCTG GCATGCCAGA CCTGGTGGAC
    TCTGGTGAAG TCCAGTCCGA TCCACACAAA TTTGCCTTTC CGTCATCCTC TTCTTCACCA
    CCAAACACAC CTGGACCTAG GGTTGCAGAG CTGCAAAGGA AGCGTGTTTC CAGTGACAGT
    TCGGTGGCAG AAGCACTTGC ACACAGTGAC TCGCTCAGAT CAGCCAAGCC AGCTCTCTGC
    AGAGCTGGGG GTGTTCATCG CTCAAATACA ACCGCAGCAG ATTTCAAACC TACCAGCAGG
    TTTGAGAAGA GCGCTGTGAA CAATGCCAAC TCTGTGTCAG TGGACAGTCT AATTGAATGT
    GTGAAAAAGA AGGACCATCA GAAGTTGAAG GAGCTGCATA AGAAAGGGGC CGACCTGTGT
    GTGCAGGACC CTGCTGGACG GACCCTATTG CACTATGCTG TGGAAGTGGG CAGCAAGGAG
    ATTGTCAAAT ACATCATTGA TAATGCACCC ACCGATATCC TGGATGTAAC AGAGAAAGAA
    AATGGAGAGA CTGTGTTGCA CAAAGCAGCC TCGCTGTGTC ATAGGACAAT CTGTCATTAC
    CTAGTGGAAG CAGGAGCATC TCTTATGAAG ACAGACTTAC AGGGTGACAC TCCGAAGCAT
    CGTGCCGAGA AGGCGAAAGA TGCTGAACTT GCGGCATATC TTGAAAACCG CCAGCACTAC
    CAAATGATCC AGCGTGAGGA CCAGGAGACT GCAGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa13286
    Clone ID Related Accession (Same CDS sequence) XM_005169037.3 , XM_005169037.4
    Accession Version XM_005169037.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3087bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product diacylglycerol kinase zeta isoform X12
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169037.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGAGCAAG AGCTGGGAGC AGGTTCTCAG GACAGGGAAG AGGTCACAGC CTCTCTGGCC 
    TCCACGTCCG CTTCTTCATC TAACTCTGAC ATCCCATCCA AGCCTTTGCC CGAGGGGGAT
    AGAGTGGCGG TTAGTCGGCG GCACAGCAGC AGGACTTTTA CTGGGTTACG ACTGTTTGGA
    AGGAGGAAGG CTATTGCCAA GTCCGGTCTC CAGCACCTCT CATGCCAGCC CAGTGCTGCA
    GTGTTAGCCA AAGGGGAAAC GGACAGAGAA ATACGCAACA CAGTAGACTG GAGTGAATCA
    GCTGTATACG GAGAGCACAT CTGGTTTGAG ACGAATGTCT CGGGGGACTT CTGTTACGTT
    GGAGAACAGC ACTGTTACGC CAAGTCACTG CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG
    GCTTGCAAGA TCGTAGTTCA CTCTATCTGC ATTGAACAGC TGGAGAAGAT AAACTTCAGA
    TGTAAACCGT CTTTCAGGGA GTCAGGATCA CGGAACATCC GAGAGCCTGC AATAGTGCGA
    CACCACTGGG TCCATCGGAG AAGACAAGAG GGCAAATGCA AACAGTGTGG AAAGGGGTTT
    CAGCAGAAAT TTGCCTTTCA CAGTAAAGAG ATTGTAGCCA TCAGCTGCTC CTGGTGCAAA
    CAAGCATATC ATAATAAGGT GACATGCTTT ATGCTGCAGC AGATAGAAGA ACCATGTTCA
    TTAGGAGCTC ATGCCGCTGT AATAGTACCT CCTACTTGGA TCATACGGGT TCGCCGACCT
    CAGTCCTCAC TAAAGTCAAG CAAAAAGAAA AAACGGACAT CTTTCAAACG TAAATCCAGC
    AAGAAAGGAG CAGAGGAGGC ACGATGGAAG CCTTTTATAG TGAGACCCAT TCCCTCCCAA
    CTCATGAAGC CACTGCTAGT GTTTGTAAAT CCAAAGAGTG GAGGAAACCA GGGAGCTAAA
    ATCATCCAGA CCTTTCTTTG GTATCTAAAC CCTCGGCAGG TGTTTGATCT AAGTCAAGGA
    GGTCCTCAGG AAGGCTTGGA AATGTATCGC AAGGTTCACA ACTTGAGAAT TTTGGCCTGT
    GGGGGGGATG GCACGGTGGG CTGGATCTTA TCTGCACTTG ATCAGCTGCA ATTAAACCCT
    TCTCCTGCTG TGGCCGTACT TCCCCTGGGC ACAGGAAATG ACCTTGCTAG GACGCTAAAC
    TGGGGTGGGG GCTACACAGA TGAACCTCTG AGTAAAATCC TGTCTCATGT GGAGGATGGG
    AATATTGTAC AGCTGGATCG GTGGAATCTT GTTGTGAAGC CCAACCCAGA GGCAGGACCA
    GAGGAAAGAG ACGAGCAGGT GACGGACAAG CTCCCTCTAG ACGTCTTCAA TAACTACTTC
    AGCCTTGGAT TTGATGCTCA TGTGACTTTA GAGTTCCACG AATCTCGAGA GGCTAATCCT
    GAGAAATTTA ACAGCCGGTT CAGAAACAAA ATGTTCTATG CAGGGACAGC GTTTTCTGAT
    TTCTTGATGG GCAGCTCCAA AGACCTGGCT AAGCACATCA AAGTAGTCTG TGACGGCACA
    GATTTAACCT CAAAGGTCCA GGATTTGAAG CTGCAATGCC TGGTCTTCCT AAATATCCCC
    AGGTACTGTG CGGGCACAAT GCCATGGGGC AATCCGAGTG AGCATCATGA CTTTGAGCCT
    CAGCGTCATG ATGATGGCTG CATTGAGGTT ATTGGATTCA CAATGACATC ACTGGCCACA
    CTGCAGGTCG GAGGTCACGG TGAGAGGTTA AATCAGTGTC GCGAAGTCAC CCTCACCACC
    TTTAAGTCAA TCCCCATGCA GGTGGACGGG GAGCCTTGTA AACTAGCACC ATCTGTTATT
    CACATTTCTT TGCGAAACCA GGCCAACATG GTGCAGAAAA CCAAGAGACG TACATCTATA
    CCACTGCTAA ACGACAGTCA GCAACCGTTT CCAGAACGAC TACGGATCAG AGTGAGCCGT
    ATCAGCATGC ATGATTATGA AGCTTTGCAC TACGACAAGG AGAAGCTCAA AGAAGCTTCG
    ATTCCACTGG GTCTCATCGT TGTCCCTGGA GACAGTGATT TGGAAACCTG CCGAGCTCAC
    ATCGAGAGAC TACAGGAGGA CTTTGTGTCG TGTAACCCGT CTCTGCAGCG GCTGGTGCTC
    CAGGAAGGTG ACGGGAGCAA ATCAAAGGCT TTGTCGTCTC AGAGGCTATC ACCGAAATGG
    TGCTTTCTCG ACTCTACAAC TGCTGATCGA TTCTACAGGA TTGATCGAGC TCAAGAGCAT
    CTCAACTACG TGACTGAAAT TTCTCAGGAT GAGCTGTTTG TTTTGGACCC AGAGCTGGTG
    GTCACAGAGA CTGTTAGCAC ATCACCTGGC ATGCCAGACC TGGTGGACTC TGGTGAAGTC
    CAGTCCGATC CACACAAATT TGCCTTTCCG TCATCCTCTT CTTCACCACC AAACACACCT
    GGACCTAGGG TTGCAGAGCT GCAAAGGAAG CGTGTTTCCA GTGACAGTTC GGTGGCAGAA
    GCACTTGCAC ACAGTGACTC GCTCAGATCA GCCAAGCCAG CTCTCTGCAG AGCTGGGGGT
    GTTCATCGCT CAAATACAAC CGCAGCAGAT TTCAAACCTA CCAGCAGGTT TGAGAAGAGC
    GCTGTGAACA ATGCCAACTC TGTGTCAGTG GACAGTCTAA TTGAATGTGT GAAAAAGAAG
    GACCATCAGA AGTTGAAGGA GCTGCATAAG AAAGGGGCCG ACCTGTGTGT GCAGGACCCT
    GCTGGACGGA CCCTATTGCA CTATGCTGTG GAAGTGGGCA GCAAGGAGAT TGTCAAATAC
    ATCATTGATA ATGCACCCAC CGATATCCTG GATGTAACAG AGAAAGAAAA TGGAGAGACT
    GTGTTGCACA AAGCAGCCTC GCTGTGTCAT AGGACAATCT GTCATTACCT AGTGGAAGCA
    GGAGCATCTC TTATGAAGAC AGACTTACAG GGTGACACTC CGAAGCATCG TGCCGAGAAG
    GCGAAAGATG CTGAACTTGC GGCATATCTT GAAAACCGCC AGCACTACCA AATGATCCAG
    CGTGAGGACC AGGAGACTGC AGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005169094.1
    CDS513..3599
    Translation

    Target ORF information:

    RefSeq Version XM_005169037.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005169037.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGAGCAAG AGCTGGGAGC AGGTTCTCAG GACAGGGAAG AGGTCACAGC CTCTCTGGCC 
    TCCACGTCCG CTTCTTCATC TAACTCTGAC ATCCCATCCA AGCCTTTGCC CGAGGGGGAT
    AGAGTGGCGG TTAGTCGGCG GCACAGCAGC AGGACTTTTA CTGGGTTACG ACTGTTTGGA
    AGGAGGAAGG CTATTGCCAA GTCCGGTCTC CAGCACCTCT CATGCCAGCC CAGTGCTGCA
    GTGTTAGCCA AAGGGGAAAC GGACAGAGAA ATACGCAACA CAGTAGACTG GAGTGAATCA
    GCTGTATACG GAGAGCACAT CTGGTTTGAG ACGAATGTCT CGGGGGACTT CTGTTACGTT
    GGAGAACAGC ACTGTTACGC CAAGTCACTG CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG
    GCTTGCAAGA TCGTAGTTCA CTCTATCTGC ATTGAACAGC TGGAGAAGAT AAACTTCAGA
    TGTAAACCGT CTTTCAGGGA GTCAGGATCA CGGAACATCC GAGAGCCTGC AATAGTGCGA
    CACCACTGGG TCCATCGGAG AAGACAAGAG GGCAAATGCA AACAGTGTGG AAAGGGGTTT
    CAGCAGAAAT TTGCCTTTCA CAGTAAAGAG ATTGTAGCCA TCAGCTGCTC CTGGTGCAAA
    CAAGCATATC ATAATAAGGT GACATGCTTT ATGCTGCAGC AGATAGAAGA ACCATGTTCA
    TTAGGAGCTC ATGCCGCTGT AATAGTACCT CCTACTTGGA TCATACGGGT TCGCCGACCT
    CAGTCCTCAC TAAAGTCAAG CAAAAAGAAA AAACGGACAT CTTTCAAACG TAAATCCAGC
    AAGAAAGGAG CAGAGGAGGC ACGATGGAAG CCTTTTATAG TGAGACCCAT TCCCTCCCAA
    CTCATGAAGC CACTGCTAGT GTTTGTAAAT CCAAAGAGTG GAGGAAACCA GGGAGCTAAA
    ATCATCCAGA CCTTTCTTTG GTATCTAAAC CCTCGGCAGG TGTTTGATCT AAGTCAAGGA
    GGTCCTCAGG AAGGCTTGGA AATGTATCGC AAGGTTCACA ACTTGAGAAT TTTGGCCTGT
    GGGGGGGATG GCACGGTGGG CTGGATCTTA TCTGCACTTG ATCAGCTGCA ATTAAACCCT
    TCTCCTGCTG TGGCCGTACT TCCCCTGGGC ACAGGAAATG ACCTTGCTAG GACGCTAAAC
    TGGGGTGGGG GCTACACAGA TGAACCTCTG AGTAAAATCC TGTCTCATGT GGAGGATGGG
    AATATTGTAC AGCTGGATCG GTGGAATCTT GTTGTGAAGC CCAACCCAGA GGCAGGACCA
    GAGGAAAGAG ACGAGCAGGT GACGGACAAG CTCCCTCTAG ACGTCTTCAA TAACTACTTC
    AGCCTTGGAT TTGATGCTCA TGTGACTTTA GAGTTCCACG AATCTCGAGA GGCTAATCCT
    GAGAAATTTA ACAGCCGGTT CAGAAACAAA ATGTTCTATG CAGGGACAGC GTTTTCTGAT
    TTCTTGATGG GCAGCTCCAA AGACCTGGCT AAGCACATCA AAGTAGTCTG TGACGGCACA
    GATTTAACCT CAAAGGTCCA GGATTTGAAG CTGCAATGCC TGGTCTTCCT AAATATCCCC
    AGGTACTGTG CGGGCACAAT GCCATGGGGC AATCCGAGTG AGCATCATGA CTTTGAGCCT
    CAGCGTCATG ATGATGGCTG CATTGAGGTT ATTGGATTCA CAATGACATC ACTGGCCACA
    CTGCAGGTCG GAGGTCACGG TGAGAGGTTA AATCAGTGTC GCGAAGTCAC CCTCACCACC
    TTTAAGTCAA TCCCCATGCA GGTGGACGGG GAGCCTTGTA AACTAGCACC ATCTGTTATT
    CACATTTCTT TGCGAAACCA GGCCAACATG GTGCAGAAAA CCAAGAGACG TACATCTATA
    CCACTGCTAA ACGACAGTCA GCAACCGTTT CCAGAACGAC TACGGATCAG AGTGAGCCGT
    ATCAGCATGC ATGATTATGA AGCTTTGCAC TACGACAAGG AGAAGCTCAA AGAAGCTTCG
    ATTCCACTGG GTCTCATCGT TGTCCCTGGA GACAGTGATT TGGAAACCTG CCGAGCTCAC
    ATCGAGAGAC TACAGGAGGA CTTTGTGTCG TGTAACCCGT CTCTGCAGCG GCTGGTGCTC
    CAGGAAGGTG ACGGGAGCAA ATCAAAGGCT TTGTCGTCTC AGAGGCTATC ACCGAAATGG
    TGCTTTCTCG ACTCTACAAC TGCTGATCGA TTCTACAGGA TTGATCGAGC TCAAGAGCAT
    CTCAACTACG TGACTGAAAT TTCTCAGGAT GAGCTGTTTG TTTTGGACCC AGAGCTGGTG
    GTCACAGAGA CTGTTAGCAC ATCACCTGGC ATGCCAGACC TGGTGGACTC TGGTGAAGTC
    CAGTCCGATC CACACAAATT TGCCTTTCCG TCATCCTCTT CTTCACCACC AAACACACCT
    GGACCTAGGG TTGCAGAGCT GCAAAGGAAG CGTGTTTCCA GTGACAGTTC GGTGGCAGAA
    GCACTTGCAC ACAGTGACTC GCTCAGATCA GCCAAGCCAG CTCTCTGCAG AGCTGGGGGT
    GTTCATCGCT CAAATACAAC CGCAGCAGAT TTCAAACCTA CCAGCAGGTT TGAGAAGAGC
    GCTGTGAACA ATGCCAACTC TGTGTCAGTG GACAGTCTAA TTGAATGTGT GAAAAAGAAG
    GACCATCAGA AGTTGAAGGA GCTGCATAAG AAAGGGGCCG ACCTGTGTGT GCAGGACCCT
    GCTGGACGGA CCCTATTGCA CTATGCTGTG GAAGTGGGCA GCAAGGAGAT TGTCAAATAC
    ATCATTGATA ATGCACCCAC CGATATCCTG GATGTAACAG AGAAAGAAAA TGGAGAGACT
    GTGTTGCACA AAGCAGCCTC GCTGTGTCAT AGGACAATCT GTCATTACCT AGTGGAAGCA
    GGAGCATCTC TTATGAAGAC AGACTTACAG GGTGACACTC CGAAGCATCG TGCCGAGAAG
    GCGAAAGATG CTGAACTTGC GGCATATCTT GAAAACCGCC AGCACTACCA AATGATCCAG
    CGTGAGGACC AGGAGACTGC AGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa13286
    Clone ID Related Accession (Same CDS sequence) XM_005169037.3 , XM_005169037.4
    Accession Version XM_005169037.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3087bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product diacylglycerol kinase zeta isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005169037.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGAGCAAG AGCTGGGAGC AGGTTCTCAG GACAGGGAAG AGGTCACAGC CTCTCTGGCC 
    TCCACGTCCG CTTCTTCATC TAACTCTGAC ATCCCATCCA AGCCTTTGCC CGAGGGGGAT
    AGAGTGGCGG TTAGTCGGCG GCACAGCAGC AGGACTTTTA CTGGGTTACG ACTGTTTGGA
    AGGAGGAAGG CTATTGCCAA GTCCGGTCTC CAGCACCTCT CATGCCAGCC CAGTGCTGCA
    GTGTTAGCCA AAGGGGAAAC GGACAGAGAA ATACGCAACA CAGTAGACTG GAGTGAATCA
    GCTGTATACG GAGAGCACAT CTGGTTTGAG ACGAATGTCT CGGGGGACTT CTGTTACGTT
    GGAGAACAGC ACTGTTACGC CAAGTCACTG CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG
    GCTTGCAAGA TCGTAGTTCA CTCTATCTGC ATTGAACAGC TGGAGAAGAT AAACTTCAGA
    TGTAAACCGT CTTTCAGGGA GTCAGGATCA CGGAACATCC GAGAGCCTGC AATAGTGCGA
    CACCACTGGG TCCATCGGAG AAGACAAGAG GGCAAATGCA AACAGTGTGG AAAGGGGTTT
    CAGCAGAAAT TTGCCTTTCA CAGTAAAGAG ATTGTAGCCA TCAGCTGCTC CTGGTGCAAA
    CAAGCATATC ATAATAAGGT GACATGCTTT ATGCTGCAGC AGATAGAAGA ACCATGTTCA
    TTAGGAGCTC ATGCCGCTGT AATAGTACCT CCTACTTGGA TCATACGGGT TCGCCGACCT
    CAGTCCTCAC TAAAGTCAAG CAAAAAGAAA AAACGGACAT CTTTCAAACG TAAATCCAGC
    AAGAAAGGAG CAGAGGAGGC ACGATGGAAG CCTTTTATAG TGAGACCCAT TCCCTCCCAA
    CTCATGAAGC CACTGCTAGT GTTTGTAAAT CCAAAGAGTG GAGGAAACCA GGGAGCTAAA
    ATCATCCAGA CCTTTCTTTG GTATCTAAAC CCTCGGCAGG TGTTTGATCT AAGTCAAGGA
    GGTCCTCAGG AAGGCTTGGA AATGTATCGC AAGGTTCACA ACTTGAGAAT TTTGGCCTGT
    GGGGGGGATG GCACGGTGGG CTGGATCTTA TCTGCACTTG ATCAGCTGCA ATTAAACCCT
    TCTCCTGCTG TGGCCGTACT TCCCCTGGGC ACAGGAAATG ACCTTGCTAG GACGCTAAAC
    TGGGGTGGGG GCTACACAGA TGAACCTCTG AGTAAAATCC TGTCTCATGT GGAGGATGGG
    AATATTGTAC AGCTGGATCG GTGGAATCTT GTTGTGAAGC CCAACCCAGA GGCAGGACCA
    GAGGAAAGAG ACGAGCAGGT GACGGACAAG CTCCCTCTAG ACGTCTTCAA TAACTACTTC
    AGCCTTGGAT TTGATGCTCA TGTGACTTTA GAGTTCCACG AATCTCGAGA GGCTAATCCT
    GAGAAATTTA ACAGCCGGTT CAGAAACAAA ATGTTCTATG CAGGGACAGC GTTTTCTGAT
    TTCTTGATGG GCAGCTCCAA AGACCTGGCT AAGCACATCA AAGTAGTCTG TGACGGCACA
    GATTTAACCT CAAAGGTCCA GGATTTGAAG CTGCAATGCC TGGTCTTCCT AAATATCCCC
    AGGTACTGTG CGGGCACAAT GCCATGGGGC AATCCGAGTG AGCATCATGA CTTTGAGCCT
    CAGCGTCATG ATGATGGCTG CATTGAGGTT ATTGGATTCA CAATGACATC ACTGGCCACA
    CTGCAGGTCG GAGGTCACGG TGAGAGGTTA AATCAGTGTC GCGAAGTCAC CCTCACCACC
    TTTAAGTCAA TCCCCATGCA GGTGGACGGG GAGCCTTGTA AACTAGCACC ATCTGTTATT
    CACATTTCTT TGCGAAACCA GGCCAACATG GTGCAGAAAA CCAAGAGACG TACATCTATA
    CCACTGCTAA ACGACAGTCA GCAACCGTTT CCAGAACGAC TACGGATCAG AGTGAGCCGT
    ATCAGCATGC ATGATTATGA AGCTTTGCAC TACGACAAGG AGAAGCTCAA AGAAGCTTCG
    ATTCCACTGG GTCTCATCGT TGTCCCTGGA GACAGTGATT TGGAAACCTG CCGAGCTCAC
    ATCGAGAGAC TACAGGAGGA CTTTGTGTCG TGTAACCCGT CTCTGCAGCG GCTGGTGCTC
    CAGGAAGGTG ACGGGAGCAA ATCAAAGGCT TTGTCGTCTC AGAGGCTATC ACCGAAATGG
    TGCTTTCTCG ACTCTACAAC TGCTGATCGA TTCTACAGGA TTGATCGAGC TCAAGAGCAT
    CTCAACTACG TGACTGAAAT TTCTCAGGAT GAGCTGTTTG TTTTGGACCC AGAGCTGGTG
    GTCACAGAGA CTGTTAGCAC ATCACCTGGC ATGCCAGACC TGGTGGACTC TGGTGAAGTC
    CAGTCCGATC CACACAAATT TGCCTTTCCG TCATCCTCTT CTTCACCACC AAACACACCT
    GGACCTAGGG TTGCAGAGCT GCAAAGGAAG CGTGTTTCCA GTGACAGTTC GGTGGCAGAA
    GCACTTGCAC ACAGTGACTC GCTCAGATCA GCCAAGCCAG CTCTCTGCAG AGCTGGGGGT
    GTTCATCGCT CAAATACAAC CGCAGCAGAT TTCAAACCTA CCAGCAGGTT TGAGAAGAGC
    GCTGTGAACA ATGCCAACTC TGTGTCAGTG GACAGTCTAA TTGAATGTGT GAAAAAGAAG
    GACCATCAGA AGTTGAAGGA GCTGCATAAG AAAGGGGCCG ACCTGTGTGT GCAGGACCCT
    GCTGGACGGA CCCTATTGCA CTATGCTGTG GAAGTGGGCA GCAAGGAGAT TGTCAAATAC
    ATCATTGATA ATGCACCCAC CGATATCCTG GATGTAACAG AGAAAGAAAA TGGAGAGACT
    GTGTTGCACA AAGCAGCCTC GCTGTGTCAT AGGACAATCT GTCATTACCT AGTGGAAGCA
    GGAGCATCTC TTATGAAGAC AGACTTACAG GGTGACACTC CGAAGCATCG TGCCGAGAAG
    GCGAAAGATG CTGAACTTGC GGCATATCTT GAAAACCGCC AGCACTACCA AATGATCCAG
    CGTGAGGACC AGGAGACTGC AGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005169094.1
    CDS702..3788
    Translation

    Target ORF information:

    RefSeq Version XM_005169037.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005169037.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGAGCAAG AGCTGGGAGC AGGTTCTCAG GACAGGGAAG AGGTCACAGC CTCTCTGGCC 
    TCCACGTCCG CTTCTTCATC TAACTCTGAC ATCCCATCCA AGCCTTTGCC CGAGGGGGAT
    AGAGTGGCGG TTAGTCGGCG GCACAGCAGC AGGACTTTTA CTGGGTTACG ACTGTTTGGA
    AGGAGGAAGG CTATTGCCAA GTCCGGTCTC CAGCACCTCT CATGCCAGCC CAGTGCTGCA
    GTGTTAGCCA AAGGGGAAAC GGACAGAGAA ATACGCAACA CAGTAGACTG GAGTGAATCA
    GCTGTATACG GAGAGCACAT CTGGTTTGAG ACGAATGTCT CGGGGGACTT CTGTTACGTT
    GGAGAACAGC ACTGTTACGC CAAGTCACTG CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG
    GCTTGCAAGA TCGTAGTTCA CTCTATCTGC ATTGAACAGC TGGAGAAGAT AAACTTCAGA
    TGTAAACCGT CTTTCAGGGA GTCAGGATCA CGGAACATCC GAGAGCCTGC AATAGTGCGA
    CACCACTGGG TCCATCGGAG AAGACAAGAG GGCAAATGCA AACAGTGTGG AAAGGGGTTT
    CAGCAGAAAT TTGCCTTTCA CAGTAAAGAG ATTGTAGCCA TCAGCTGCTC CTGGTGCAAA
    CAAGCATATC ATAATAAGGT GACATGCTTT ATGCTGCAGC AGATAGAAGA ACCATGTTCA
    TTAGGAGCTC ATGCCGCTGT AATAGTACCT CCTACTTGGA TCATACGGGT TCGCCGACCT
    CAGTCCTCAC TAAAGTCAAG CAAAAAGAAA AAACGGACAT CTTTCAAACG TAAATCCAGC
    AAGAAAGGAG CAGAGGAGGC ACGATGGAAG CCTTTTATAG TGAGACCCAT TCCCTCCCAA
    CTCATGAAGC CACTGCTAGT GTTTGTAAAT CCAAAGAGTG GAGGAAACCA GGGAGCTAAA
    ATCATCCAGA CCTTTCTTTG GTATCTAAAC CCTCGGCAGG TGTTTGATCT AAGTCAAGGA
    GGTCCTCAGG AAGGCTTGGA AATGTATCGC AAGGTTCACA ACTTGAGAAT TTTGGCCTGT
    GGGGGGGATG GCACGGTGGG CTGGATCTTA TCTGCACTTG ATCAGCTGCA ATTAAACCCT
    TCTCCTGCTG TGGCCGTACT TCCCCTGGGC ACAGGAAATG ACCTTGCTAG GACGCTAAAC
    TGGGGTGGGG GCTACACAGA TGAACCTCTG AGTAAAATCC TGTCTCATGT GGAGGATGGG
    AATATTGTAC AGCTGGATCG GTGGAATCTT GTTGTGAAGC CCAACCCAGA GGCAGGACCA
    GAGGAAAGAG ACGAGCAGGT GACGGACAAG CTCCCTCTAG ACGTCTTCAA TAACTACTTC
    AGCCTTGGAT TTGATGCTCA TGTGACTTTA GAGTTCCACG AATCTCGAGA GGCTAATCCT
    GAGAAATTTA ACAGCCGGTT CAGAAACAAA ATGTTCTATG CAGGGACAGC GTTTTCTGAT
    TTCTTGATGG GCAGCTCCAA AGACCTGGCT AAGCACATCA AAGTAGTCTG TGACGGCACA
    GATTTAACCT CAAAGGTCCA GGATTTGAAG CTGCAATGCC TGGTCTTCCT AAATATCCCC
    AGGTACTGTG CGGGCACAAT GCCATGGGGC AATCCGAGTG AGCATCATGA CTTTGAGCCT
    CAGCGTCATG ATGATGGCTG CATTGAGGTT ATTGGATTCA CAATGACATC ACTGGCCACA
    CTGCAGGTCG GAGGTCACGG TGAGAGGTTA AATCAGTGTC GCGAAGTCAC CCTCACCACC
    TTTAAGTCAA TCCCCATGCA GGTGGACGGG GAGCCTTGTA AACTAGCACC ATCTGTTATT
    CACATTTCTT TGCGAAACCA GGCCAACATG GTGCAGAAAA CCAAGAGACG TACATCTATA
    CCACTGCTAA ACGACAGTCA GCAACCGTTT CCAGAACGAC TACGGATCAG AGTGAGCCGT
    ATCAGCATGC ATGATTATGA AGCTTTGCAC TACGACAAGG AGAAGCTCAA AGAAGCTTCG
    ATTCCACTGG GTCTCATCGT TGTCCCTGGA GACAGTGATT TGGAAACCTG CCGAGCTCAC
    ATCGAGAGAC TACAGGAGGA CTTTGTGTCG TGTAACCCGT CTCTGCAGCG GCTGGTGCTC
    CAGGAAGGTG ACGGGAGCAA ATCAAAGGCT TTGTCGTCTC AGAGGCTATC ACCGAAATGG
    TGCTTTCTCG ACTCTACAAC TGCTGATCGA TTCTACAGGA TTGATCGAGC TCAAGAGCAT
    CTCAACTACG TGACTGAAAT TTCTCAGGAT GAGCTGTTTG TTTTGGACCC AGAGCTGGTG
    GTCACAGAGA CTGTTAGCAC ATCACCTGGC ATGCCAGACC TGGTGGACTC TGGTGAAGTC
    CAGTCCGATC CACACAAATT TGCCTTTCCG TCATCCTCTT CTTCACCACC AAACACACCT
    GGACCTAGGG TTGCAGAGCT GCAAAGGAAG CGTGTTTCCA GTGACAGTTC GGTGGCAGAA
    GCACTTGCAC ACAGTGACTC GCTCAGATCA GCCAAGCCAG CTCTCTGCAG AGCTGGGGGT
    GTTCATCGCT CAAATACAAC CGCAGCAGAT TTCAAACCTA CCAGCAGGTT TGAGAAGAGC
    GCTGTGAACA ATGCCAACTC TGTGTCAGTG GACAGTCTAA TTGAATGTGT GAAAAAGAAG
    GACCATCAGA AGTTGAAGGA GCTGCATAAG AAAGGGGCCG ACCTGTGTGT GCAGGACCCT
    GCTGGACGGA CCCTATTGCA CTATGCTGTG GAAGTGGGCA GCAAGGAGAT TGTCAAATAC
    ATCATTGATA ATGCACCCAC CGATATCCTG GATGTAACAG AGAAAGAAAA TGGAGAGACT
    GTGTTGCACA AAGCAGCCTC GCTGTGTCAT AGGACAATCT GTCATTACCT AGTGGAAGCA
    GGAGCATCTC TTATGAAGAC AGACTTACAG GGTGACACTC CGAAGCATCG TGCCGAGAAG
    GCGAAAGATG CTGAACTTGC GGCATATCTT GAAAACCGCC AGCACTACCA AATGATCCAG
    CGTGAGGACC AGGAGACTGC AGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa41929
    Clone ID Related Accession (Same CDS sequence) XM_009303361.2
    Accession Version XM_009303361.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3786bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product diacylglycerol kinase zeta isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303361.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
    CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT
    TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG
    CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA
    GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT
    CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC
    CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC
    CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA
    TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA
    CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC
    CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT
    GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG
    ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC
    AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC
    CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA
    ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG
    ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG
    CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC
    ATTGAACAGC TGGAGAAGTT ATTGGATTGG AATGCTCCAC CTCAGGGGGA GGGGCTTGAT
    GAGATGCCTC TCAAAGAGCC GTCATTCAGA ATAAACTTCA GATGTAAACC GTCTTTCAGG
    GAGTCAGGAT CACGGAACAT CCGAGAGCCT GCAATAGTGC GACACCACTG GGTCCATCGG
    AGAAGACAAG AGGGCAAATG CAAACAGTGT GGAAAGGGGT TTCAGCAGAA ATTTGCCTTT
    CACAGTAAAG AGATTGTAGC CATCAGCTGC TCCTGGTGCA AACAAGCATA TCATAATAAG
    GTGACATGCT TTATGCTGCA GCAGATAGAA GAACCATGTT CATTAGGAGC TCATGCCGCT
    GTAATAGTAC CTCCTACTTG GATCATACGG GTTCGCCGAC CTCAGTCCTC ACTAAAGTCA
    AGCAAAAAGA AAAAACGGAC ATCTTTCAAA CGTAAATCCA GCAAGAAAGG AGCAGAGGAG
    GCACGATGGA AGCCTTTTAT AGTGAGACCC ATTCCCTCCC AACTCATGAA GCCACTGCTA
    GTGTTTGTAA ATCCAAAGAG TGGAGGAAAC CAGGGAGCTA AAATCATCCA GACCTTTCTT
    TGGTATCTAA ACCCTCGGCA GGTGTTTGAT CTAAGTCAAG GAGGTCCTCA GGAAGGCTTG
    GAAATGTATC GCAAGGTTCA CAACTTGAGA ATTTTGGCCT GTGGGGGGGA TGGCACGGTG
    GGCTGGATCT TATCTGCACT TGATCAGCTG CAATTAAACC CTTCTCCTGC TGTGGCCGTA
    CTTCCCCTGG GCACAGGAAA TGACCTTGCT AGGACGCTAA ACTGGGGTGG GGGCTACACA
    GATGAACCTC TGAGTAAAAT CCTGTCTCAT GTGGAGGATG GGAATATTGT ACAGCTGGAT
    CGGTGGAATC TTGTTGTGAA GCCCAACCCA GAGGCAGGAC CAGAGGAAAG AGACGAGCAG
    GTGACGGACA AGCTCCCTCT AGACGTCTTC AATAACTACT TCAGCCTTGG ATTTGATGCT
    CATGTGACTT TAGAGTTCCA CGAATCTCGA GAGGCTAATC CTGAGAAATT TAACAGCCGG
    TTCAGAAACA AAATGTTCTA TGCAGGGACA GCGTTTTCTG ATTTCTTGAT GGGCAGCTCC
    AAAGACCTGG CTAAGCACAT CAAAGTAGTC TGTGACGGCA CAGATTTAAC CTCAAAGGTC
    CAGGATTTGA AGCTGCAATG CCTGGTCTTC CTAAATATCC CCAGGTACTG TGCGGGCACA
    ATGCCATGGG GCAATCCGAG TGAGCATCAT GACTTTGAGC CTCAGCGTCA TGATGATGGC
    TGCATTGAGG TTATTGGATT CACAATGACA TCACTGGCCA CACTGCAGGT CGGAGGTCAC
    GGTGAGAGGT TAAATCAGTG TCGCGAAGTC ACCCTCACCA CCTTTAAGTC AATCCCCATG
    CAGGTGGACG GGGAGCCTTG TAAACTAGCA CCATCTGTTA TTCACATTTC TTTGCGAAAC
    CAGGCCAACA TGGTGCAGAA AACCAAGAGA CGTACATCTA TACCACTGCT AAACGATCAG
    CAACCGTTTC CAGAACGACT ACGGATCAGA GTGAGCCGTA TCAGCATGCA TGATTATGAA
    GCTTTGCACT ACGACAAGGA GAAGCTCAAA GAAGCTTCGA TTCCACTGGG TCTCATCGTT
    GTCCCTGGAG ACAGTGATTT GGAAACCTGC CGAGCTCACA TCGAGAGACT ACAGGAGGAC
    TTTGTGTCGT GTAACCCGTC TCTGCAGCGG CTGGTGCTCC AGGAAGGTGA CGGGAGCAAA
    TCAAAGGCTT TGTCGTCTCA GAGGCTATCA CCGAAATGGT GCTTTCTCGA CTCTACAACT
    GCTGATCGAT TCTACAGGAT TGATCGAGCT CAAGAGCATC TCAACTACGT GACTGAAATT
    TCTCAGGATG AGCTGTTTGT TTTGGACCCA GAGCTGGTGG TCACAGAGAC TGTTAGCACA
    TCACCTGGCA TGCCAGACCT GGTGGACTCT GGTGAAGTCC AGTCCGATCC ACACAAATTT
    GCCTTTCCGT CATCCTCTTC TTCACCACCA AACACACCTG GACCTAGGGT TGCAGAGCTG
    CAAAGGAAGC GTGTTTCCAG TGACAGTTCG GTGGCAGAAG CACTTGCACA CAGTGACTCG
    CTCAGATCAG CCAAGCCAGC TCTCTGCAGA GCTGGGGGTG TTCATCGCTC AAATACAACC
    GCAGCAGATT TCAAACCTAC CAGCAGGTTT GAGAAGAGCG CTGTGAACAA TGCCAACTCT
    GTGTCAGTGG ACAGTCTAAT TGAATGTGTG AAAAAGAAGG ACCATCAGAA GTTGAAGGAG
    CTGCATAAGA AAGGGGCCGA CCTGTGTGTG CAGGACCCTG CTGGACGGAC CCTATTGCAC
    TATGCTGTGG AAGTGGGCAG CAAGGAGATT GTCAAATACA TCATTGATAA TGCACCCACC
    GATATCCTGG ATGTAACAGA GAAAGAAAAT GGAGAGACTG TGTTGCACAA AGCAGCCTCG
    CTGTGTCATA GGACAATCTG TCATTACCTA GTGGAAGCAG GAGCATCTCT TATGAAGACA
    GACTTACAGG GTGACACTCC GAAGCATCGT GCCGAGAAGG CGAAAGATGC TGAACTTGCG
    GCATATCTTG AAAACCGCCA GCACTACCAA ATGATCCAGC GTGAGGACCA GGAGACTGCA
    GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301636.1
    CDS136..3921
    Translation

    Target ORF information:

    RefSeq Version XM_009303361.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303361.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
    CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT
    TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG
    CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA
    GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT
    CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC
    CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC
    CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA
    TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA
    CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC
    CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT
    GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG
    ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC
    AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC
    CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA
    ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG
    ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG
    CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC
    ATTGAACAGC TGGAGAAGTT ATTGGATTGG AATGCTCCAC CTCAGGGGGA GGGGCTTGAT
    GAGATGCCTC TCAAAGAGCC GTCATTCAGA ATAAACTTCA GATGTAAACC GTCTTTCAGG
    GAGTCAGGAT CACGGAACAT CCGAGAGCCT GCAATAGTGC GACACCACTG GGTCCATCGG
    AGAAGACAAG AGGGCAAATG CAAACAGTGT GGAAAGGGGT TTCAGCAGAA ATTTGCCTTT
    CACAGTAAAG AGATTGTAGC CATCAGCTGC TCCTGGTGCA AACAAGCATA TCATAATAAG
    GTGACATGCT TTATGCTGCA GCAGATAGAA GAACCATGTT CATTAGGAGC TCATGCCGCT
    GTAATAGTAC CTCCTACTTG GATCATACGG GTTCGCCGAC CTCAGTCCTC ACTAAAGTCA
    AGCAAAAAGA AAAAACGGAC ATCTTTCAAA CGTAAATCCA GCAAGAAAGG AGCAGAGGAG
    GCACGATGGA AGCCTTTTAT AGTGAGACCC ATTCCCTCCC AACTCATGAA GCCACTGCTA
    GTGTTTGTAA ATCCAAAGAG TGGAGGAAAC CAGGGAGCTA AAATCATCCA GACCTTTCTT
    TGGTATCTAA ACCCTCGGCA GGTGTTTGAT CTAAGTCAAG GAGGTCCTCA GGAAGGCTTG
    GAAATGTATC GCAAGGTTCA CAACTTGAGA ATTTTGGCCT GTGGGGGGGA TGGCACGGTG
    GGCTGGATCT TATCTGCACT TGATCAGCTG CAATTAAACC CTTCTCCTGC TGTGGCCGTA
    CTTCCCCTGG GCACAGGAAA TGACCTTGCT AGGACGCTAA ACTGGGGTGG GGGCTACACA
    GATGAACCTC TGAGTAAAAT CCTGTCTCAT GTGGAGGATG GGAATATTGT ACAGCTGGAT
    CGGTGGAATC TTGTTGTGAA GCCCAACCCA GAGGCAGGAC CAGAGGAAAG AGACGAGCAG
    GTGACGGACA AGCTCCCTCT AGACGTCTTC AATAACTACT TCAGCCTTGG ATTTGATGCT
    CATGTGACTT TAGAGTTCCA CGAATCTCGA GAGGCTAATC CTGAGAAATT TAACAGCCGG
    TTCAGAAACA AAATGTTCTA TGCAGGGACA GCGTTTTCTG ATTTCTTGAT GGGCAGCTCC
    AAAGACCTGG CTAAGCACAT CAAAGTAGTC TGTGACGGCA CAGATTTAAC CTCAAAGGTC
    CAGGATTTGA AGCTGCAATG CCTGGTCTTC CTAAATATCC CCAGGTACTG TGCGGGCACA
    ATGCCATGGG GCAATCCGAG TGAGCATCAT GACTTTGAGC CTCAGCGTCA TGATGATGGC
    TGCATTGAGG TTATTGGATT CACAATGACA TCACTGGCCA CACTGCAGGT CGGAGGTCAC
    GGTGAGAGGT TAAATCAGTG TCGCGAAGTC ACCCTCACCA CCTTTAAGTC AATCCCCATG
    CAGGTGGACG GGGAGCCTTG TAAACTAGCA CCATCTGTTA TTCACATTTC TTTGCGAAAC
    CAGGCCAACA TGGTGCAGAA AACCAAGAGA CGTACATCTA TACCACTGCT AAACGATCAG
    CAACCGTTTC CAGAACGACT ACGGATCAGA GTGAGCCGTA TCAGCATGCA TGATTATGAA
    GCTTTGCACT ACGACAAGGA GAAGCTCAAA GAAGCTTCGA TTCCACTGGG TCTCATCGTT
    GTCCCTGGAG ACAGTGATTT GGAAACCTGC CGAGCTCACA TCGAGAGACT ACAGGAGGAC
    TTTGTGTCGT GTAACCCGTC TCTGCAGCGG CTGGTGCTCC AGGAAGGTGA CGGGAGCAAA
    TCAAAGGCTT TGTCGTCTCA GAGGCTATCA CCGAAATGGT GCTTTCTCGA CTCTACAACT
    GCTGATCGAT TCTACAGGAT TGATCGAGCT CAAGAGCATC TCAACTACGT GACTGAAATT
    TCTCAGGATG AGCTGTTTGT TTTGGACCCA GAGCTGGTGG TCACAGAGAC TGTTAGCACA
    TCACCTGGCA TGCCAGACCT GGTGGACTCT GGTGAAGTCC AGTCCGATCC ACACAAATTT
    GCCTTTCCGT CATCCTCTTC TTCACCACCA AACACACCTG GACCTAGGGT TGCAGAGCTG
    CAAAGGAAGC GTGTTTCCAG TGACAGTTCG GTGGCAGAAG CACTTGCACA CAGTGACTCG
    CTCAGATCAG CCAAGCCAGC TCTCTGCAGA GCTGGGGGTG TTCATCGCTC AAATACAACC
    GCAGCAGATT TCAAACCTAC CAGCAGGTTT GAGAAGAGCG CTGTGAACAA TGCCAACTCT
    GTGTCAGTGG ACAGTCTAAT TGAATGTGTG AAAAAGAAGG ACCATCAGAA GTTGAAGGAG
    CTGCATAAGA AAGGGGCCGA CCTGTGTGTG CAGGACCCTG CTGGACGGAC CCTATTGCAC
    TATGCTGTGG AAGTGGGCAG CAAGGAGATT GTCAAATACA TCATTGATAA TGCACCCACC
    GATATCCTGG ATGTAACAGA GAAAGAAAAT GGAGAGACTG TGTTGCACAA AGCAGCCTCG
    CTGTGTCATA GGACAATCTG TCATTACCTA GTGGAAGCAG GAGCATCTCT TATGAAGACA
    GACTTACAGG GTGACACTCC GAAGCATCGT GCCGAGAAGG CGAAAGATGC TGAACTTGCG
    GCATATCTTG AAAACCGCCA GCACTACCAA ATGATCCAGC GTGAGGACCA GGAGACTGCA
    GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa41930
    Clone ID Related Accession (Same CDS sequence) XM_009303362.3 , XM_009303362.2
    Accession Version XM_009303362.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3714bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product diacylglycerol kinase zeta isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303362.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
    CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT
    TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG
    CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA
    GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT
    CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC
    CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC
    CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA
    TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA
    CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC
    CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT
    GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG
    ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC
    AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC
    CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA
    ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG
    ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG
    CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC
    ATTGAACAGC TGGAGAAGAT AAACTTCAGA TGTAAACCGT CTTTCAGGGA GTCAGGATCA
    CGGAACATCC GAGAGCCTGC AATAGTGCGA CACCACTGGG TCCATCGGAG AAGACAAGAG
    GGCAAATGCA AACAGTGTGG AAAGGGGTTT CAGCAGAAAT TTGCCTTTCA CAGTAAAGAG
    ATTGTAGCCA TCAGCTGCTC CTGGTGCAAA CAAGCATATC ATAATAAGGT GACATGCTTT
    ATGCTGCAGC AGATAGAAGA ACCATGTTCA TTAGGAGCTC ATGCCGCTGT AATAGTACCT
    CCTACTTGGA TCATACGGGT TCGCCGACCT CAGTCCTCAC TAAAGTCAAG CAAAAAGAAA
    AAACGGACAT CTTTCAAACG TAAATCCAGC AAGAAAGGAG CAGAGGAGGC ACGATGGAAG
    CCTTTTATAG TGAGACCCAT TCCCTCCCAA CTCATGAAGC CACTGCTAGT GTTTGTAAAT
    CCAAAGAGTG GAGGAAACCA GGGAGCTAAA ATCATCCAGA CCTTTCTTTG GTATCTAAAC
    CCTCGGCAGG TGTTTGATCT AAGTCAAGGA GGTCCTCAGG AAGGCTTGGA AATGTATCGC
    AAGGTTCACA ACTTGAGAAT TTTGGCCTGT GGGGGGGATG GCACGGTGGG CTGGATCTTA
    TCTGCACTTG ATCAGCTGCA ATTAAACCCT TCTCCTGCTG TGGCCGTACT TCCCCTGGGC
    ACAGGAAATG ACCTTGCTAG GACGCTAAAC TGGGGTGGGG GCTACACAGA TGAACCTCTG
    AGTAAAATCC TGTCTCATGT GGAGGATGGG AATATTGTAC AGCTGGATCG GTGGAATCTT
    GTTGTGAAGC CCAACCCAGA GGCAGGACCA GAGGAAAGAG ACGAGCAGGT GACGGACAAG
    CTCCCTCTAG ACGTCTTCAA TAACTACTTC AGCCTTGGAT TTGATGCTCA TGTGACTTTA
    GAGTTCCACG AATCTCGAGA GGCTAATCCT GAGAAATTTA ACAGCCGGTT CAGAAACAAA
    ATGTTCTATG CAGGGACAGC GTTTTCTGAT TTCTTGATGG GCAGCTCCAA AGACCTGGCT
    AAGCACATCA AAGTAGTCTG TGACGGCACA GATTTAACCT CAAAGGTCCA GGATTTGAAG
    CTGCAATGCC TGGTCTTCCT AAATATCCCC AGGTACTGTG CGGGCACAAT GCCATGGGGC
    AATCCGAGTG AGCATCATGA CTTTGAGCCT CAGCGTCATG ATGATGGCTG CATTGAGGTT
    ATTGGATTCA CAATGACATC ACTGGCCACA CTGCAGGTCG GAGGTCACGG TGAGAGGTTA
    AATCAGTGTC GCGAAGTCAC CCTCACCACC TTTAAGTCAA TCCCCATGCA GGTGGACGGG
    GAGCCTTGTA AACTAGCACC ATCTGTTATT CACATTTCTT TGCGAAACCA GGCCAACATG
    GTGCAGAAAA CCAAGAGACG TACATCTATA CCACTGCTAA ACGATCAGCA ACCGTTTCCA
    GAACGACTAC GGATCAGAGT GAGCCGTATC AGCATGCATG ATTATGAAGC TTTGCACTAC
    GACAAGGAGA AGCTCAAAGA AGCTTCGATT CCACTGGGTC TCATCGTTGT CCCTGGAGAC
    AGTGATTTGG AAACCTGCCG AGCTCACATC GAGAGACTAC AGGAGGACTT TGTGTCGTGT
    AACCCGTCTC TGCAGCGGCT GGTGCTCCAG GAAGGTGACG GGAGCAAATC AAAGGCTTTG
    TCGTCTCAGA GGCTATCACC GAAATGGTGC TTTCTCGACT CTACAACTGC TGATCGATTC
    TACAGGATTG ATCGAGCTCA AGAGCATCTC AACTACGTGA CTGAAATTTC TCAGGATGAG
    CTGTTTGTTT TGGACCCAGA GCTGGTGGTC ACAGAGACTG TTAGCACATC ACCTGGCATG
    CCAGACCTGG TGGACTCTGG TGAAGTCCAG TCCGATCCAC ACAAATTTGC CTTTCCGTCA
    TCCTCTTCTT CACCACCAAA CACACCTGGA CCTAGGGTTG CAGAGCTGCA AAGGAAGCGT
    GTTTCCAGTG ACAGTTCGGT GGCAGAAGCA CTTGCACACA GTGACTCGCT CAGATCAGCC
    AAGCCAGCTC TCTGCAGAGC TGGGGGTGTT CATCGCTCAA ATACAACCGC AGCAGATTTC
    AAACCTACCA GCAGGTTTGA GAAGAGCGCT GTGAACAATG CCAACTCTGT GTCAGTGGAC
    AGTCTAATTG AATGTGTGAA AAAGAAGGAC CATCAGAAGT TGAAGGAGCT GCATAAGAAA
    GGGGCCGACC TGTGTGTGCA GGACCCTGCT GGACGGACCC TATTGCACTA TGCTGTGGAA
    GTGGGCAGCA AGGAGATTGT CAAATACATC ATTGATAATG CACCCACCGA TATCCTGGAT
    GTAACAGAGA AAGAAAATGG AGAGACTGTG TTGCACAAAG CAGCCTCGCT GTGTCATAGG
    ACAATCTGTC ATTACCTAGT GGAAGCAGGA GCATCTCTTA TGAAGACAGA CTTACAGGGT
    GACACTCCGA AGCATCGTGC CGAGAAGGCG AAAGATGCTG AACTTGCGGC ATATCTTGAA
    AACCGCCAGC ACTACCAAAT GATCCAGCGT GAGGACCAGG AGACTGCAGT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301637.1
    CDS170..3883
    Translation

    Target ORF information:

    RefSeq Version XM_009303362.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303362.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
    CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT
    TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG
    CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA
    GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT
    CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC
    CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC
    CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA
    TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA
    CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC
    CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT
    GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG
    ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC
    AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC
    CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA
    ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG
    ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG
    CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC
    ATTGAACAGC TGGAGAAGAT AAACTTCAGA TGTAAACCGT CTTTCAGGGA GTCAGGATCA
    CGGAACATCC GAGAGCCTGC AATAGTGCGA CACCACTGGG TCCATCGGAG AAGACAAGAG
    GGCAAATGCA AACAGTGTGG AAAGGGGTTT CAGCAGAAAT TTGCCTTTCA CAGTAAAGAG
    ATTGTAGCCA TCAGCTGCTC CTGGTGCAAA CAAGCATATC ATAATAAGGT GACATGCTTT
    ATGCTGCAGC AGATAGAAGA ACCATGTTCA TTAGGAGCTC ATGCCGCTGT AATAGTACCT
    CCTACTTGGA TCATACGGGT TCGCCGACCT CAGTCCTCAC TAAAGTCAAG CAAAAAGAAA
    AAACGGACAT CTTTCAAACG TAAATCCAGC AAGAAAGGAG CAGAGGAGGC ACGATGGAAG
    CCTTTTATAG TGAGACCCAT TCCCTCCCAA CTCATGAAGC CACTGCTAGT GTTTGTAAAT
    CCAAAGAGTG GAGGAAACCA GGGAGCTAAA ATCATCCAGA CCTTTCTTTG GTATCTAAAC
    CCTCGGCAGG TGTTTGATCT AAGTCAAGGA GGTCCTCAGG AAGGCTTGGA AATGTATCGC
    AAGGTTCACA ACTTGAGAAT TTTGGCCTGT GGGGGGGATG GCACGGTGGG CTGGATCTTA
    TCTGCACTTG ATCAGCTGCA ATTAAACCCT TCTCCTGCTG TGGCCGTACT TCCCCTGGGC
    ACAGGAAATG ACCTTGCTAG GACGCTAAAC TGGGGTGGGG GCTACACAGA TGAACCTCTG
    AGTAAAATCC TGTCTCATGT GGAGGATGGG AATATTGTAC AGCTGGATCG GTGGAATCTT
    GTTGTGAAGC CCAACCCAGA GGCAGGACCA GAGGAAAGAG ACGAGCAGGT GACGGACAAG
    CTCCCTCTAG ACGTCTTCAA TAACTACTTC AGCCTTGGAT TTGATGCTCA TGTGACTTTA
    GAGTTCCACG AATCTCGAGA GGCTAATCCT GAGAAATTTA ACAGCCGGTT CAGAAACAAA
    ATGTTCTATG CAGGGACAGC GTTTTCTGAT TTCTTGATGG GCAGCTCCAA AGACCTGGCT
    AAGCACATCA AAGTAGTCTG TGACGGCACA GATTTAACCT CAAAGGTCCA GGATTTGAAG
    CTGCAATGCC TGGTCTTCCT AAATATCCCC AGGTACTGTG CGGGCACAAT GCCATGGGGC
    AATCCGAGTG AGCATCATGA CTTTGAGCCT CAGCGTCATG ATGATGGCTG CATTGAGGTT
    ATTGGATTCA CAATGACATC ACTGGCCACA CTGCAGGTCG GAGGTCACGG TGAGAGGTTA
    AATCAGTGTC GCGAAGTCAC CCTCACCACC TTTAAGTCAA TCCCCATGCA GGTGGACGGG
    GAGCCTTGTA AACTAGCACC ATCTGTTATT CACATTTCTT TGCGAAACCA GGCCAACATG
    GTGCAGAAAA CCAAGAGACG TACATCTATA CCACTGCTAA ACGATCAGCA ACCGTTTCCA
    GAACGACTAC GGATCAGAGT GAGCCGTATC AGCATGCATG ATTATGAAGC TTTGCACTAC
    GACAAGGAGA AGCTCAAAGA AGCTTCGATT CCACTGGGTC TCATCGTTGT CCCTGGAGAC
    AGTGATTTGG AAACCTGCCG AGCTCACATC GAGAGACTAC AGGAGGACTT TGTGTCGTGT
    AACCCGTCTC TGCAGCGGCT GGTGCTCCAG GAAGGTGACG GGAGCAAATC AAAGGCTTTG
    TCGTCTCAGA GGCTATCACC GAAATGGTGC TTTCTCGACT CTACAACTGC TGATCGATTC
    TACAGGATTG ATCGAGCTCA AGAGCATCTC AACTACGTGA CTGAAATTTC TCAGGATGAG
    CTGTTTGTTT TGGACCCAGA GCTGGTGGTC ACAGAGACTG TTAGCACATC ACCTGGCATG
    CCAGACCTGG TGGACTCTGG TGAAGTCCAG TCCGATCCAC ACAAATTTGC CTTTCCGTCA
    TCCTCTTCTT CACCACCAAA CACACCTGGA CCTAGGGTTG CAGAGCTGCA AAGGAAGCGT
    GTTTCCAGTG ACAGTTCGGT GGCAGAAGCA CTTGCACACA GTGACTCGCT CAGATCAGCC
    AAGCCAGCTC TCTGCAGAGC TGGGGGTGTT CATCGCTCAA ATACAACCGC AGCAGATTTC
    AAACCTACCA GCAGGTTTGA GAAGAGCGCT GTGAACAATG CCAACTCTGT GTCAGTGGAC
    AGTCTAATTG AATGTGTGAA AAAGAAGGAC CATCAGAAGT TGAAGGAGCT GCATAAGAAA
    GGGGCCGACC TGTGTGTGCA GGACCCTGCT GGACGGACCC TATTGCACTA TGCTGTGGAA
    GTGGGCAGCA AGGAGATTGT CAAATACATC ATTGATAATG CACCCACCGA TATCCTGGAT
    GTAACAGAGA AAGAAAATGG AGAGACTGTG TTGCACAAAG CAGCCTCGCT GTGTCATAGG
    ACAATCTGTC ATTACCTAGT GGAAGCAGGA GCATCTCTTA TGAAGACAGA CTTACAGGGT
    GACACTCCGA AGCATCGTGC CGAGAAGGCG AAAGATGCTG AACTTGCGGC ATATCTTGAA
    AACCGCCAGC ACTACCAAAT GATCCAGCGT GAGGACCAGG AGACTGCAGT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa41930
    Clone ID Related Accession (Same CDS sequence) XM_009303362.3 , XM_009303362.2
    Accession Version XM_009303362.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3714bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product diacylglycerol kinase zeta isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303362.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
    CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT
    TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG
    CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA
    GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT
    CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC
    CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC
    CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA
    TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA
    CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC
    CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT
    GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG
    ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC
    AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC
    CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA
    ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG
    ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG
    CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC
    ATTGAACAGC TGGAGAAGAT AAACTTCAGA TGTAAACCGT CTTTCAGGGA GTCAGGATCA
    CGGAACATCC GAGAGCCTGC AATAGTGCGA CACCACTGGG TCCATCGGAG AAGACAAGAG
    GGCAAATGCA AACAGTGTGG AAAGGGGTTT CAGCAGAAAT TTGCCTTTCA CAGTAAAGAG
    ATTGTAGCCA TCAGCTGCTC CTGGTGCAAA CAAGCATATC ATAATAAGGT GACATGCTTT
    ATGCTGCAGC AGATAGAAGA ACCATGTTCA TTAGGAGCTC ATGCCGCTGT AATAGTACCT
    CCTACTTGGA TCATACGGGT TCGCCGACCT CAGTCCTCAC TAAAGTCAAG CAAAAAGAAA
    AAACGGACAT CTTTCAAACG TAAATCCAGC AAGAAAGGAG CAGAGGAGGC ACGATGGAAG
    CCTTTTATAG TGAGACCCAT TCCCTCCCAA CTCATGAAGC CACTGCTAGT GTTTGTAAAT
    CCAAAGAGTG GAGGAAACCA GGGAGCTAAA ATCATCCAGA CCTTTCTTTG GTATCTAAAC
    CCTCGGCAGG TGTTTGATCT AAGTCAAGGA GGTCCTCAGG AAGGCTTGGA AATGTATCGC
    AAGGTTCACA ACTTGAGAAT TTTGGCCTGT GGGGGGGATG GCACGGTGGG CTGGATCTTA
    TCTGCACTTG ATCAGCTGCA ATTAAACCCT TCTCCTGCTG TGGCCGTACT TCCCCTGGGC
    ACAGGAAATG ACCTTGCTAG GACGCTAAAC TGGGGTGGGG GCTACACAGA TGAACCTCTG
    AGTAAAATCC TGTCTCATGT GGAGGATGGG AATATTGTAC AGCTGGATCG GTGGAATCTT
    GTTGTGAAGC CCAACCCAGA GGCAGGACCA GAGGAAAGAG ACGAGCAGGT GACGGACAAG
    CTCCCTCTAG ACGTCTTCAA TAACTACTTC AGCCTTGGAT TTGATGCTCA TGTGACTTTA
    GAGTTCCACG AATCTCGAGA GGCTAATCCT GAGAAATTTA ACAGCCGGTT CAGAAACAAA
    ATGTTCTATG CAGGGACAGC GTTTTCTGAT TTCTTGATGG GCAGCTCCAA AGACCTGGCT
    AAGCACATCA AAGTAGTCTG TGACGGCACA GATTTAACCT CAAAGGTCCA GGATTTGAAG
    CTGCAATGCC TGGTCTTCCT AAATATCCCC AGGTACTGTG CGGGCACAAT GCCATGGGGC
    AATCCGAGTG AGCATCATGA CTTTGAGCCT CAGCGTCATG ATGATGGCTG CATTGAGGTT
    ATTGGATTCA CAATGACATC ACTGGCCACA CTGCAGGTCG GAGGTCACGG TGAGAGGTTA
    AATCAGTGTC GCGAAGTCAC CCTCACCACC TTTAAGTCAA TCCCCATGCA GGTGGACGGG
    GAGCCTTGTA AACTAGCACC ATCTGTTATT CACATTTCTT TGCGAAACCA GGCCAACATG
    GTGCAGAAAA CCAAGAGACG TACATCTATA CCACTGCTAA ACGATCAGCA ACCGTTTCCA
    GAACGACTAC GGATCAGAGT GAGCCGTATC AGCATGCATG ATTATGAAGC TTTGCACTAC
    GACAAGGAGA AGCTCAAAGA AGCTTCGATT CCACTGGGTC TCATCGTTGT CCCTGGAGAC
    AGTGATTTGG AAACCTGCCG AGCTCACATC GAGAGACTAC AGGAGGACTT TGTGTCGTGT
    AACCCGTCTC TGCAGCGGCT GGTGCTCCAG GAAGGTGACG GGAGCAAATC AAAGGCTTTG
    TCGTCTCAGA GGCTATCACC GAAATGGTGC TTTCTCGACT CTACAACTGC TGATCGATTC
    TACAGGATTG ATCGAGCTCA AGAGCATCTC AACTACGTGA CTGAAATTTC TCAGGATGAG
    CTGTTTGTTT TGGACCCAGA GCTGGTGGTC ACAGAGACTG TTAGCACATC ACCTGGCATG
    CCAGACCTGG TGGACTCTGG TGAAGTCCAG TCCGATCCAC ACAAATTTGC CTTTCCGTCA
    TCCTCTTCTT CACCACCAAA CACACCTGGA CCTAGGGTTG CAGAGCTGCA AAGGAAGCGT
    GTTTCCAGTG ACAGTTCGGT GGCAGAAGCA CTTGCACACA GTGACTCGCT CAGATCAGCC
    AAGCCAGCTC TCTGCAGAGC TGGGGGTGTT CATCGCTCAA ATACAACCGC AGCAGATTTC
    AAACCTACCA GCAGGTTTGA GAAGAGCGCT GTGAACAATG CCAACTCTGT GTCAGTGGAC
    AGTCTAATTG AATGTGTGAA AAAGAAGGAC CATCAGAAGT TGAAGGAGCT GCATAAGAAA
    GGGGCCGACC TGTGTGTGCA GGACCCTGCT GGACGGACCC TATTGCACTA TGCTGTGGAA
    GTGGGCAGCA AGGAGATTGT CAAATACATC ATTGATAATG CACCCACCGA TATCCTGGAT
    GTAACAGAGA AAGAAAATGG AGAGACTGTG TTGCACAAAG CAGCCTCGCT GTGTCATAGG
    ACAATCTGTC ATTACCTAGT GGAAGCAGGA GCATCTCTTA TGAAGACAGA CTTACAGGGT
    GACACTCCGA AGCATCGTGC CGAGAAGGCG AAAGATGCTG AACTTGCGGC ATATCTTGAA
    AACCGCCAGC ACTACCAAAT GATCCAGCGT GAGGACCAGG AGACTGCAGT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301637.1
    CDS136..3849
    Translation

    Target ORF information:

    RefSeq Version XM_009303362.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303362.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
    CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT
    TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG
    CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA
    GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT
    CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC
    CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC
    CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA
    TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA
    CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC
    CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT
    GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG
    ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC
    AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC
    CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA
    ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG
    ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG
    CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC
    ATTGAACAGC TGGAGAAGAT AAACTTCAGA TGTAAACCGT CTTTCAGGGA GTCAGGATCA
    CGGAACATCC GAGAGCCTGC AATAGTGCGA CACCACTGGG TCCATCGGAG AAGACAAGAG
    GGCAAATGCA AACAGTGTGG AAAGGGGTTT CAGCAGAAAT TTGCCTTTCA CAGTAAAGAG
    ATTGTAGCCA TCAGCTGCTC CTGGTGCAAA CAAGCATATC ATAATAAGGT GACATGCTTT
    ATGCTGCAGC AGATAGAAGA ACCATGTTCA TTAGGAGCTC ATGCCGCTGT AATAGTACCT
    CCTACTTGGA TCATACGGGT TCGCCGACCT CAGTCCTCAC TAAAGTCAAG CAAAAAGAAA
    AAACGGACAT CTTTCAAACG TAAATCCAGC AAGAAAGGAG CAGAGGAGGC ACGATGGAAG
    CCTTTTATAG TGAGACCCAT TCCCTCCCAA CTCATGAAGC CACTGCTAGT GTTTGTAAAT
    CCAAAGAGTG GAGGAAACCA GGGAGCTAAA ATCATCCAGA CCTTTCTTTG GTATCTAAAC
    CCTCGGCAGG TGTTTGATCT AAGTCAAGGA GGTCCTCAGG AAGGCTTGGA AATGTATCGC
    AAGGTTCACA ACTTGAGAAT TTTGGCCTGT GGGGGGGATG GCACGGTGGG CTGGATCTTA
    TCTGCACTTG ATCAGCTGCA ATTAAACCCT TCTCCTGCTG TGGCCGTACT TCCCCTGGGC
    ACAGGAAATG ACCTTGCTAG GACGCTAAAC TGGGGTGGGG GCTACACAGA TGAACCTCTG
    AGTAAAATCC TGTCTCATGT GGAGGATGGG AATATTGTAC AGCTGGATCG GTGGAATCTT
    GTTGTGAAGC CCAACCCAGA GGCAGGACCA GAGGAAAGAG ACGAGCAGGT GACGGACAAG
    CTCCCTCTAG ACGTCTTCAA TAACTACTTC AGCCTTGGAT TTGATGCTCA TGTGACTTTA
    GAGTTCCACG AATCTCGAGA GGCTAATCCT GAGAAATTTA ACAGCCGGTT CAGAAACAAA
    ATGTTCTATG CAGGGACAGC GTTTTCTGAT TTCTTGATGG GCAGCTCCAA AGACCTGGCT
    AAGCACATCA AAGTAGTCTG TGACGGCACA GATTTAACCT CAAAGGTCCA GGATTTGAAG
    CTGCAATGCC TGGTCTTCCT AAATATCCCC AGGTACTGTG CGGGCACAAT GCCATGGGGC
    AATCCGAGTG AGCATCATGA CTTTGAGCCT CAGCGTCATG ATGATGGCTG CATTGAGGTT
    ATTGGATTCA CAATGACATC ACTGGCCACA CTGCAGGTCG GAGGTCACGG TGAGAGGTTA
    AATCAGTGTC GCGAAGTCAC CCTCACCACC TTTAAGTCAA TCCCCATGCA GGTGGACGGG
    GAGCCTTGTA AACTAGCACC ATCTGTTATT CACATTTCTT TGCGAAACCA GGCCAACATG
    GTGCAGAAAA CCAAGAGACG TACATCTATA CCACTGCTAA ACGATCAGCA ACCGTTTCCA
    GAACGACTAC GGATCAGAGT GAGCCGTATC AGCATGCATG ATTATGAAGC TTTGCACTAC
    GACAAGGAGA AGCTCAAAGA AGCTTCGATT CCACTGGGTC TCATCGTTGT CCCTGGAGAC
    AGTGATTTGG AAACCTGCCG AGCTCACATC GAGAGACTAC AGGAGGACTT TGTGTCGTGT
    AACCCGTCTC TGCAGCGGCT GGTGCTCCAG GAAGGTGACG GGAGCAAATC AAAGGCTTTG
    TCGTCTCAGA GGCTATCACC GAAATGGTGC TTTCTCGACT CTACAACTGC TGATCGATTC
    TACAGGATTG ATCGAGCTCA AGAGCATCTC AACTACGTGA CTGAAATTTC TCAGGATGAG
    CTGTTTGTTT TGGACCCAGA GCTGGTGGTC ACAGAGACTG TTAGCACATC ACCTGGCATG
    CCAGACCTGG TGGACTCTGG TGAAGTCCAG TCCGATCCAC ACAAATTTGC CTTTCCGTCA
    TCCTCTTCTT CACCACCAAA CACACCTGGA CCTAGGGTTG CAGAGCTGCA AAGGAAGCGT
    GTTTCCAGTG ACAGTTCGGT GGCAGAAGCA CTTGCACACA GTGACTCGCT CAGATCAGCC
    AAGCCAGCTC TCTGCAGAGC TGGGGGTGTT CATCGCTCAA ATACAACCGC AGCAGATTTC
    AAACCTACCA GCAGGTTTGA GAAGAGCGCT GTGAACAATG CCAACTCTGT GTCAGTGGAC
    AGTCTAATTG AATGTGTGAA AAAGAAGGAC CATCAGAAGT TGAAGGAGCT GCATAAGAAA
    GGGGCCGACC TGTGTGTGCA GGACCCTGCT GGACGGACCC TATTGCACTA TGCTGTGGAA
    GTGGGCAGCA AGGAGATTGT CAAATACATC ATTGATAATG CACCCACCGA TATCCTGGAT
    GTAACAGAGA AAGAAAATGG AGAGACTGTG TTGCACAAAG CAGCCTCGCT GTGTCATAGG
    ACAATCTGTC ATTACCTAGT GGAAGCAGGA GCATCTCTTA TGAAGACAGA CTTACAGGGT
    GACACTCCGA AGCATCGTGC CGAGAAGGCG AAAGATGCTG AACTTGCGGC ATATCTTGAA
    AACCGCCAGC ACTACCAAAT GATCCAGCGT GAGGACCAGG AGACTGCAGT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.