dgkza gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_009303362.3	XP_009301637.1	diacylglycerol kinase zeta isoform X4
XM_005169036.3	XP_005169093.1	diacylglycerol kinase zeta isoform X11
XM_005169037.3	XP_005169094.1	diacylglycerol kinase zeta isoform X12
XM_005169029.3	XP_005169086.1	diacylglycerol kinase zeta isoform X1
XM_005169031.3	XP_005169088.1	diacylglycerol kinase zeta isoform X4
XM_009303361.2	XP_009301636.1	diacylglycerol kinase zeta isoform X2
XM_005169032.3	XP_005169089.1	diacylglycerol kinase zeta isoform X5
XM_005169033.3	XP_005169090.1	diacylglycerol kinase zeta isoform X6
XM_005169034.3	XP_005169091.1	diacylglycerol kinase zeta isoform X8
XM_005169037.4	XP_005169094.1	diacylglycerol kinase zeta isoform X7
XM_005169033.4	XP_005169090.1	diacylglycerol kinase zeta isoform X3
XM_005169034.4	XP_005169091.1	diacylglycerol kinase zeta isoform X5
XM_001922410.6	XP_001922445.3	diacylglycerol kinase zeta isoform X6
XM_021477929.1	XP_021333604.1	diacylglycerol kinase zeta isoform X8
XM_005169030.3	XP_005169087.1	diacylglycerol kinase zeta isoform X3
XM_009303362.2	XP_009301637.1	diacylglycerol kinase zeta isoform X7
XM_005169035.3	XP_005169092.1	diacylglycerol kinase zeta isoform X9
XM_001922410.5	XP_001922445.3	diacylglycerol kinase zeta isoform X10
XM_021477928.1	XP_021333603.1	diacylglycerol kinase zeta isoform X1
XM_005169029.4	XP_005169086.1	diacylglycerol kinase zeta isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa143498	XM_021477928.1 Latest version!	Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa143499	XM_021477929.1 Latest version!	Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa13277	XM_005169029.4 Latest version!	Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa13278	XM_005169030.3 Latest version!	Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa13279	XM_005169031.3 Latest version!	Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa13280	XM_005169032.3 Latest version!	Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa13281	XM_005169033.4 Latest version!	Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa13282	XM_005169034.4 Latest version!	Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa13283	XM_005169035.3 Latest version!	Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa13284	XM_001922410.6 Latest version!	Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa13285	XM_005169036.3 Latest version!	Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa13286	XM_005169037.4 Latest version!	Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa41929	XM_009303361.2 Latest version!	Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa41930	XM_009303362.3 Latest version!	Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
ODa13286	XM_005169037.3	Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa13281	XM_005169033.3	Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa13282	XM_005169034.3	Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa41930	XM_009303362.2	Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa13284	XM_001922410.5	Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa13277	XM_005169029.3	Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

dgkza ( XM_021477928.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333603.1 Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa143498
Clone ID Related Accession (Same CDS sequence)	XM_021477928.1
Accession Version	XM_021477928.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	diacylglycerol kinase zeta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT 
TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG 
CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA 
GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT 
CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC 
CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC 
CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA 
TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA 
CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC 
CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT 
GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG 
ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC 
AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC 
CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA 
ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG 
ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG 
CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC 
ATTGAACAGC TGGAGAAGTT ATTGGATTGG AATGCTCCAC CTCAGGGGGA GGGGCTTGAT 
GAGATGCCTC TCAAAGAGCC GTCATTCAGA ATAAACTTCA GATGTAAACC GTCTTTCAGG 
GAGTCAGGAT CACGGAACAT CCGAGAGCAA ACCTGGCGAC GCAGAGCTTT CAGAGGTCGC 
TGTGCTTTGG AGCAAATGAG AGGTGCTGCA GCTAAATGTA CTGCGCACAC CTCTAACACA 
CAGCCTGCAA TAGTGCGACA CCACTGGGTC CATCGGAGAA GACAAGAGGG CAAATGCAAA 
CAGTGTGGAA AGGGGTTTCA GCAGAAATTT GCCTTTCACA GTAAAGAGAT TGTAGCCATC 
AGCTGCTCCT GGTGCAAACA AGCATATCAT AATAAGGTGA CATGCTTTAT GCTGCAGCAG 
ATAGAAGAAC CATGTTCATT AGGAGCTCAT GCCGCTGTAA TAGTACCTCC TACTTGGATC 
ATACGGGTTC GCCGACCTCA GTCCTCACTA AAGTCAAGCA AAAAGAAAAA ACGGACATCT 
TTCAAACGTA AATCCAGCAA GAAAGGAGCA GAGGAGGCAC GATGGAAGCC TTTTATAGTG 
AGACCCATTC CCTCCCAACT CATGAAGCCA CTGCTAGTGT TTGTAAATCC AAAGAGTGGA 
GGAAACCAGG GAGCTAAAAT CATCCAGACC TTTCTTTGGT ATCTAAACCC TCGGCAGGTG 
TTTGATCTAA GTCAAGGAGG TCCTCAGGAA GGCTTGGAAA TGTATCGCAA GGTTCACAAC 
TTGAGAATTT TGGCCTGTGG GGGGGATGGC ACGGTGGGCT GGATCTTATC TGCACTTGAT 
CAGCTGCAAT TAAACCCTTC TCCTGCTGTG GCCGTACTTC CCCTGGGCAC AGGAAATGAC 
CTTGCTAGGA CGCTAAACTG GGGTGGGGGC TACACAGATG AACCTCTGAG TAAAATCCTG 
TCTCATGTGG AGGATGGGAA TATTGTACAG CTGGATCGGT GGAATCTTGT TGTGAAGCCC 
AACCCAGAGG CAGGACCAGA GGAAAGAGAC GAGCAGGTGA CGGACAAGCT CCCTCTAGAC 
GTCTTCAATA ACTACTTCAG CCTTGGATTT GATGCTCATG TGACTTTAGA GTTCCACGAA 
TCTCGAGAGG CTAATCCTGA GAAATTTAAC AGCCGGTTCA GAAACAAAAT GTTCTATGCA 
GGGACAGCGT TTTCTGATTT CTTGATGGGC AGCTCCAAAG ACCTGGCTAA GCACATCAAA 
GTAGTCTGTG ACGGCACAGA TTTAACCTCA AAGGTCCAGG ATTTGAAGCT GCAATGCCTG 
GTCTTCCTAA ATATCCCCAG GTACTGTGCG GGCACAATGC CATGGGGCAA TCCGAGTGAG 
CATCATGACT TTGAGCCTCA GCGTCATGAT GATGGCTGCA TTGAGGTTAT TGGATTCACA 
ATGACATCAC TGGCCACACT GCAGGTCGGA GGTCACGGTG AGAGGTTAAA TCAGTGTCGC 
GAAGTCACCC TCACCACCTT TAAGTCAATC CCCATGCAGG TGGACGGGGA GCCTTGTAAA 
CTAGCACCAT CTGTTATTCA CATTTCTTTG CGAAACCAGG CCAACATGGT GCAGAAAACC 
AAGAGACGTA CATCTATACC ACTGCTAAAC GACAGTCAGC AACCGTTTCC AGAACGACTA 
CGGATCAGAG TGAGCCGTAT CAGCATGCAT GATTATGAAG CTTTGCACTA CGACAAGGAG 
AAGCTCAAAG AAGCTTCGAT TCCACTGGGT CTCATCGTTG TCCCTGGAGA CAGTGATTTG 
GAAACCTGCC GAGCTCACAT CGAGAGACTA CAGGAGGACT TTGTGTCGTG TAACCCGTCT 
CTGCAGCGGC TGGTGCTCCA GGAAGGTGAC GGGAGCAAAT CAAAGGCTTT GTCGTCTCAG 
AGGCTATCAC CGAAATGGTG CTTTCTCGAC TCTACAACTG CTGATCGATT CTACAGGATT 
GATCGAGCTC AAGAGCATCT CAACTACGTG ACTGAAATTT CTCAGGATGA GCTGTTTGTT 
TTGGACCCAG AGCTGGTGGT CACAGAGACT GTTAGCACAT CACCTGGCAT GCCAGACCTG 
GTGGACTCTG GTGAAGTCCA GTCCGATCCA CACAAATTTG CCTTTCCGTC ATCCTCTTCT 
TCACCACCAA ACACACCTGG ACCTAGGGTT GCAGAGCTGC AAAGGAAGCG TGTTTCCAGT 
GACAGTTCGG TGGCAGAAGC ACTTGCACAC AGTGACTCGC TCAGATCAGC CAAGCCAGCT 
CTCTGCAGAG CTGGGGGTGT TCATCGCTCA AATACAACCG CAGCAGATTT CAAACCTACC 
AGCAGGTTTG AGAAGAGCGC TGTGAACAAT GCCAACTCTG TGTCAGTGGA CAGTCTAATT 
GAATGTGTGA AAAAGAAGGA CCATCAGAAG TTGAAGGAGC TGCATAAGAA AGGGGCCGAC 
CTGTGTGTGC AGGACCCTGC TGGACGGACC CTATTGCACT ATGCTGTGGA AGTGGGCAGC 
AAGGAGATTG TCAAATACAT CATTGATAAT GCACCCACCG ATATCCTGGA TGTAACAGAG 
AAAGAAAATG GAGAGACTGT GTTGCACAAA GCAGCCTCGC TGTGTCATAG GACAATCTGT 
CATTACCTAG TGGAAGCAGG AGCATCTCTT ATGAAGACAG ACTTACAGGG TGACACTCCG 
AAGCATCGTG CCGAGAAGGC GAAAGATGCT GAACTTGCGG CATATCTTGA AAACCGCCAG 
CACTACCAAA TGATCCAGCG TGAGGACCAG GAGACTGCAG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333603.1
CDS	172..4056
Translation	MDALFNLRFRRKAVEPSGRRRSSVSLPASKARRRSSAGLPSAGLAHRRRSSAQLQIGSIQPGLVLHRRGHFTRRKSSGGTACLTPRFAIRRRQAAKHRSIHAQLLGPSLLLASVVQMSEDHEPEDKSAEEYSSLSDTDCSQDEHEQTGMDFETESDKCLTWLAQGLLVGESIQPRPLIRAPRCLRRNSSHLLPAEAVYRSPASYGLYGRYRRTSQVHNMGGLWAGKRSSLAARGSVALYRTSPPCWPDLSVSHTLQGTYYNPQIDTWSGFLSKAIAKSGLQHLSCQPSAAVLAKGETDREIRNTVDWSESAVYGEHIWFETNVSGDFCYVGEQHCYAKSLQKSVARKKCAACKIVVHSICIEQLEKLLDWNAPPQGEGLDEMPLKEPSFRINFRCKPSFRESGSRNIREQTWRRRAFRGRCALEQMRGAAAKCTAHTSNTQPAIVRHHWVHRRRQEGKCKQCGKGFQQKFAFHSKEIVAISCSWCKQAYHNKVTCFMLQQIEEPCSLGAHAAVIVPPTWIIRVRRPQSSLKSSKKKKRTSFKRKSSKKGAEEARWKPFIVRPIPSQLMKPLLVFVNPKSGGNQGAKIIQTFLWYLNPRQVFDLSQGGPQEGLEMYRKVHNLRILACGGDGTVGWILSALDQLQLNPSPAVAVLPLGTGNDLARTLNWGGGYTDEPLSKILSHVEDGNIVQLDRWNLVVKPNPEAGPEERDEQVTDKLPLDVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGTAFSDFLMGSSKDLAKHIKVVCDGTDLTSKVQDLKLQCLVFLNIPRYCAGTMPWGNPSEHHDFEPQRHDDGCIEVIGFTMTSLATLQVGGHGERLNQCREVTLTTFKSIPMQVDGEPCKLAPSVIHISLRNQANMVQKTKRRTSIPLLNDSQQPFPERLRIRVSRISMHDYEALHYDKEKLKEASIPLGLIVVPGDSDLETCRAHIERLQEDFVSCNPSLQRLVLQEGDGSKSKALSSQRLSPKWCFLDSTTADRFYRIDRAQEHLNYVTEISQDELFVLDPELVVTETVSTSPGMPDLVDSGEVQSDPHKFAFPSSSSSPPNTPGPRVAELQRKRVSSDSSVAEALAHSDSLRSAKPALCRAGGVHRSNTTAADFKPTSRFEKSAVNNANSVSVDSLIECVKKKDHQKLKELHKKGADLCVQDPAGRTLLHYAVEVGSKEIVKYIIDNAPTDILDVTEKENGETVLHKAASLCHRTICHYLVEAGASLMKTDLQGDTPKHRAEKAKDAELAAYLENRQHYQMIQREDQETAV

Target ORF information:

RefSeq Version	XM_021477928.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477928.1

ORF Insert Sequence:

ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT 
TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG 
CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA 
GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT 
CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC 
CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC 
CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA 
TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA 
CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC 
CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT 
GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG 
ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC 
AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC 
CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA 
ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG 
ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG 
CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC 
ATTGAACAGC TGGAGAAGTT ATTGGATTGG AATGCTCCAC CTCAGGGGGA GGGGCTTGAT 
GAGATGCCTC TCAAAGAGCC GTCATTCAGA ATAAACTTCA GATGTAAACC GTCTTTCAGG 
GAGTCAGGAT CACGGAACAT CCGAGAGCAA ACCTGGCGAC GCAGAGCTTT CAGAGGTCGC 
TGTGCTTTGG AGCAAATGAG AGGTGCTGCA GCTAAATGTA CTGCGCACAC CTCTAACACA 
CAGCCTGCAA TAGTGCGACA CCACTGGGTC CATCGGAGAA GACAAGAGGG CAAATGCAAA 
CAGTGTGGAA AGGGGTTTCA GCAGAAATTT GCCTTTCACA GTAAAGAGAT TGTAGCCATC 
AGCTGCTCCT GGTGCAAACA AGCATATCAT AATAAGGTGA CATGCTTTAT GCTGCAGCAG 
ATAGAAGAAC CATGTTCATT AGGAGCTCAT GCCGCTGTAA TAGTACCTCC TACTTGGATC 
ATACGGGTTC GCCGACCTCA GTCCTCACTA AAGTCAAGCA AAAAGAAAAA ACGGACATCT 
TTCAAACGTA AATCCAGCAA GAAAGGAGCA GAGGAGGCAC GATGGAAGCC TTTTATAGTG 
AGACCCATTC CCTCCCAACT CATGAAGCCA CTGCTAGTGT TTGTAAATCC AAAGAGTGGA 
GGAAACCAGG GAGCTAAAAT CATCCAGACC TTTCTTTGGT ATCTAAACCC TCGGCAGGTG 
TTTGATCTAA GTCAAGGAGG TCCTCAGGAA GGCTTGGAAA TGTATCGCAA GGTTCACAAC 
TTGAGAATTT TGGCCTGTGG GGGGGATGGC ACGGTGGGCT GGATCTTATC TGCACTTGAT 
CAGCTGCAAT TAAACCCTTC TCCTGCTGTG GCCGTACTTC CCCTGGGCAC AGGAAATGAC 
CTTGCTAGGA CGCTAAACTG GGGTGGGGGC TACACAGATG AACCTCTGAG TAAAATCCTG 
TCTCATGTGG AGGATGGGAA TATTGTACAG CTGGATCGGT GGAATCTTGT TGTGAAGCCC 
AACCCAGAGG CAGGACCAGA GGAAAGAGAC GAGCAGGTGA CGGACAAGCT CCCTCTAGAC 
GTCTTCAATA ACTACTTCAG CCTTGGATTT GATGCTCATG TGACTTTAGA GTTCCACGAA 
TCTCGAGAGG CTAATCCTGA GAAATTTAAC AGCCGGTTCA GAAACAAAAT GTTCTATGCA 
GGGACAGCGT TTTCTGATTT CTTGATGGGC AGCTCCAAAG ACCTGGCTAA GCACATCAAA 
GTAGTCTGTG ACGGCACAGA TTTAACCTCA AAGGTCCAGG ATTTGAAGCT GCAATGCCTG 
GTCTTCCTAA ATATCCCCAG GTACTGTGCG GGCACAATGC CATGGGGCAA TCCGAGTGAG 
CATCATGACT TTGAGCCTCA GCGTCATGAT GATGGCTGCA TTGAGGTTAT TGGATTCACA 
ATGACATCAC TGGCCACACT GCAGGTCGGA GGTCACGGTG AGAGGTTAAA TCAGTGTCGC 
GAAGTCACCC TCACCACCTT TAAGTCAATC CCCATGCAGG TGGACGGGGA GCCTTGTAAA 
CTAGCACCAT CTGTTATTCA CATTTCTTTG CGAAACCAGG CCAACATGGT GCAGAAAACC 
AAGAGACGTA CATCTATACC ACTGCTAAAC GACAGTCAGC AACCGTTTCC AGAACGACTA 
CGGATCAGAG TGAGCCGTAT CAGCATGCAT GATTATGAAG CTTTGCACTA CGACAAGGAG 
AAGCTCAAAG AAGCTTCGAT TCCACTGGGT CTCATCGTTG TCCCTGGAGA CAGTGATTTG 
GAAACCTGCC GAGCTCACAT CGAGAGACTA CAGGAGGACT TTGTGTCGTG TAACCCGTCT 
CTGCAGCGGC TGGTGCTCCA GGAAGGTGAC GGGAGCAAAT CAAAGGCTTT GTCGTCTCAG 
AGGCTATCAC CGAAATGGTG CTTTCTCGAC TCTACAACTG CTGATCGATT CTACAGGATT 
GATCGAGCTC AAGAGCATCT CAACTACGTG ACTGAAATTT CTCAGGATGA GCTGTTTGTT 
TTGGACCCAG AGCTGGTGGT CACAGAGACT GTTAGCACAT CACCTGGCAT GCCAGACCTG 
GTGGACTCTG GTGAAGTCCA GTCCGATCCA CACAAATTTG CCTTTCCGTC ATCCTCTTCT 
TCACCACCAA ACACACCTGG ACCTAGGGTT GCAGAGCTGC AAAGGAAGCG TGTTTCCAGT 
GACAGTTCGG TGGCAGAAGC ACTTGCACAC AGTGACTCGC TCAGATCAGC CAAGCCAGCT 
CTCTGCAGAG CTGGGGGTGT TCATCGCTCA AATACAACCG CAGCAGATTT CAAACCTACC 
AGCAGGTTTG AGAAGAGCGC TGTGAACAAT GCCAACTCTG TGTCAGTGGA CAGTCTAATT 
GAATGTGTGA AAAAGAAGGA CCATCAGAAG TTGAAGGAGC TGCATAAGAA AGGGGCCGAC 
CTGTGTGTGC AGGACCCTGC TGGACGGACC CTATTGCACT ATGCTGTGGA AGTGGGCAGC 
AAGGAGATTG TCAAATACAT CATTGATAAT GCACCCACCG ATATCCTGGA TGTAACAGAG 
AAAGAAAATG GAGAGACTGT GTTGCACAAA GCAGCCTCGC TGTGTCATAG GACAATCTGT 
CATTACCTAG TGGAAGCAGG AGCATCTCTT ATGAAGACAG ACTTACAGGG TGACACTCCG 
AAGCATCGTG CCGAGAAGGC GAAAGATGCT GAACTTGCGG CATATCTTGA AAACCGCCAG 
CACTACCAAA TGATCCAGCG TGAGGACCAG GAGACTGCAG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dgkza ( XM_021477929.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333604.1 Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X8, mRNA.

Price & Availability↑

CloneID	ODa143499
Clone ID Related Accession (Same CDS sequence)	XM_021477929.1
Accession Version	XM_021477929.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	diacylglycerol kinase zeta isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCTC ATAGGAGGAA ATGGGTCTTG CCCCCCACCC CTCCGCATCA ACCGCACCAC 
AGGAAGGCTA TTGCCAAGTC CGGTCTCCAG CACCTCTCAT GCCAGCCCAG TGCTGCAGTG 
TTAGCCAAAG GGGAAACGGA CAGAGAAATA CGCAACACAG TAGACTGGAG TGAATCAGCT 
GTATACGGAG AGCACATCTG GTTTGAGACG AATGTCTCGG GGGACTTCTG TTACGTTGGA 
GAACAGCACT GTTACGCCAA GTCACTGCAA AAGTCTGTTG CTCGGAAAAA GTGTGCGGCT 
TGCAAGATCG TAGTTCACTC TATCTGCATT GAACAGCTGG AGAAGATAAA CTTCAGATGT 
AAACCGTCTT TCAGGGAGTC AGGATCACGG AACATCCGAG AGCAAACCTG GCGACGCAGA 
GCTTTCAGAG GTCGCTGTGC TTTGGAGCAA ATGAGAGGTG CTGCAGCTAA ATGTACTGCG 
CACACCTCTA ACACACAGCC TGCAATAGTG CGACACCACT GGGTCCATCG GAGAAGACAA 
GAGGGCAAAT GCAAACAGTG TGGAAAGGGG TTTCAGCAGA AATTTGCCTT TCACAGTAAA 
GAGATTGTAG CCATCAGCTG CTCCTGGTGC AAACAAGCAT ATCATAATAA GGTGACATGC 
TTTATGCTGC AGCAGATAGA AGAACCATGT TCATTAGGAG CTCATGCCGC TGTAATAGTA 
CCTCCTACTT GGATCATACG GGTTCGCCGA CCTCAGTCCT CACTAAAGTC AAGCAAAAAG 
AAAAAACGGA CATCTTTCAA ACGTAAATCC AGCAAGAAAG GAGCAGAGGA GGCACGATGG 
AAGCCTTTTA TAGTGAGACC CATTCCCTCC CAACTCATGA AGCCACTGCT AGTGTTTGTA 
AATCCAAAGA GTGGAGGAAA CCAGGGAGCT AAAATCATCC AGACCTTTCT TTGGTATCTA 
AACCCTCGGC AGGTGTTTGA TCTAAGTCAA GGAGGTCCTC AGGAAGGCTT GGAAATGTAT 
CGCAAGGTTC ACAACTTGAG AATTTTGGCC TGTGGGGGGG ATGGCACGGT GGGCTGGATC 
TTATCTGCAC TTGATCAGCT GCAATTAAAC CCTTCTCCTG CTGTGGCCGT ACTTCCCCTG 
GGCACAGGAA ATGACCTTGC TAGGACGCTA AACTGGGGTG GGGGCTACAC AGATGAACCT 
CTGAGTAAAA TCCTGTCTCA TGTGGAGGAT GGGAATATTG TACAGCTGGA TCGGTGGAAT 
CTTGTTGTGA AGCCCAACCC AGAGGCAGGA CCAGAGGAAA GAGACGAGCA GGTGACGGAC 
AAGCTCCCTC TAGACGTCTT CAATAACTAC TTCAGCCTTG GATTTGATGC TCATGTGACT 
TTAGAGTTCC ACGAATCTCG AGAGGCTAAT CCTGAGAAAT TTAACAGCCG GTTCAGAAAC 
AAAATGTTCT ATGCAGGGAC AGCGTTTTCT GATTTCTTGA TGGGCAGCTC CAAAGACCTG 
GCTAAGCACA TCAAAGTAGT CTGTGACGGC ACAGATTTAA CCTCAAAGGT CCAGGATTTG 
AAGCTGCAAT GCCTGGTCTT CCTAAATATC CCCAGGTACT GTGCGGGCAC AATGCCATGG 
GGCAATCCGA GTGAGCATCA TGACTTTGAG CCTCAGCGTC ATGATGATGG CTGCATTGAG 
GTTATTGGAT TCACAATGAC ATCACTGGCC ACACTGCAGG TCGGAGGTCA CGGTGAGAGG 
TTAAATCAGT GTCGCGAAGT CACCCTCACC ACCTTTAAGT CAATCCCCAT GCAGGTGGAC 
GGGGAGCCTT GTAAACTAGC ACCATCTGTT ATTCACATTT CTTTGCGAAA CCAGGCCAAC 
ATGGTGCAGA AAACCAAGAG ACGTACATCT ATACCACTGC TAAACGACAG TCAGCAACCG 
TTTCCAGAAC GACTACGGAT CAGAGTGAGC CGTATCAGCA TGCATGATTA TGAAGCTTTG 
CACTACGACA AGGAGAAGCT CAAAGAAGCT TCGATTCCAC TGGGTCTCAT CGTTGTCCCT 
GGAGACAGTG ATTTGGAAAC CTGCCGAGCT CACATCGAGA GACTACAGGA GGACTTTGTG 
TCGTGTAACC CGTCTCTGCA GCGGCTGGTG CTCCAGGAAG GTGACGGGAG CAAATCAAAG 
GCTTTGTCGT CTCAGAGGCT ATCACCGAAA TGGTGCTTTC TCGACTCTAC AACTGCTGAT 
CGATTCTACA GGATTGATCG AGCTCAAGAG CATCTCAACT ACGTGACTGA AATTTCTCAG 
GATGAGCTGT TTGTTTTGGA CCCAGAGCTG GTGGTCACAG AGACTGTTAG CACATCACCT 
GGCATGCCAG ACCTGGTGGA CTCTGGTGAA GTCCAGTCCG ATCCACACAA ATTTGCCTTT 
CCGTCATCCT CTTCTTCACC ACCAAACACA CCTGGACCTA GGGTTGCAGA GCTGCAAAGG 
AAGCGTGTTT CCAGTGACAG TTCGGTGGCA GAAGCACTTG CACACAGTGA CTCGCTCAGA 
TCAGCCAAGC CAGCTCTCTG CAGAGCTGGG GGTGTTCATC GCTCAAATAC AACCGCAGCA 
GATTTCAAAC CTACCAGCAG GTTTGAGAAG AGCGCTGTGA ACAATGCCAA CTCTGTGTCA 
GTGGACAGTC TAATTGAATG TGTGAAAAAG AAGGACCATC AGAAGTTGAA GGAGCTGCAT 
AAGAAAGGGG CCGACCTGTG TGTGCAGGAC CCTGCTGGAC GGACCCTATT GCACTATGCT 
GTGGAAGTGG GCAGCAAGGA GATTGTCAAA TACATCATTG ATAATGCACC CACCGATATC 
CTGGATGTAA CAGAGAAAGA AAATGGAGAG ACTGTGTTGC ACAAAGCAGC CTCGCTGTGT 
CATAGGACAA TCTGTCATTA CCTAGTGGAA GCAGGAGCAT CTCTTATGAA GACAGACTTA 
CAGGGTGACA CTCCGAAGCA TCGTGCCGAG AAGGCGAAAG ATGCTGAACT TGCGGCATAT 
CTTGAAAACC GCCAGCACTA CCAAATGATC CAGCGTGAGG ACCAGGAGAC TGCAGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333604.1
CDS	151..3210
Translation	MWAHRRKWVLPPTPPHQPHHRKAIAKSGLQHLSCQPSAAVLAKGETDREIRNTVDWSESAVYGEHIWFETNVSGDFCYVGEQHCYAKSLQKSVARKKCAACKIVVHSICIEQLEKINFRCKPSFRESGSRNIREQTWRRRAFRGRCALEQMRGAAAKCTAHTSNTQPAIVRHHWVHRRRQEGKCKQCGKGFQQKFAFHSKEIVAISCSWCKQAYHNKVTCFMLQQIEEPCSLGAHAAVIVPPTWIIRVRRPQSSLKSSKKKKRTSFKRKSSKKGAEEARWKPFIVRPIPSQLMKPLLVFVNPKSGGNQGAKIIQTFLWYLNPRQVFDLSQGGPQEGLEMYRKVHNLRILACGGDGTVGWILSALDQLQLNPSPAVAVLPLGTGNDLARTLNWGGGYTDEPLSKILSHVEDGNIVQLDRWNLVVKPNPEAGPEERDEQVTDKLPLDVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGTAFSDFLMGSSKDLAKHIKVVCDGTDLTSKVQDLKLQCLVFLNIPRYCAGTMPWGNPSEHHDFEPQRHDDGCIEVIGFTMTSLATLQVGGHGERLNQCREVTLTTFKSIPMQVDGEPCKLAPSVIHISLRNQANMVQKTKRRTSIPLLNDSQQPFPERLRIRVSRISMHDYEALHYDKEKLKEASIPLGLIVVPGDSDLETCRAHIERLQEDFVSCNPSLQRLVLQEGDGSKSKALSSQRLSPKWCFLDSTTADRFYRIDRAQEHLNYVTEISQDELFVLDPELVVTETVSTSPGMPDLVDSGEVQSDPHKFAFPSSSSSPPNTPGPRVAELQRKRVSSDSSVAEALAHSDSLRSAKPALCRAGGVHRSNTTAADFKPTSRFEKSAVNNANSVSVDSLIECVKKKDHQKLKELHKKGADLCVQDPAGRTLLHYAVEVGSKEIVKYIIDNAPTDILDVTEKENGETVLHKAASLCHRTICHYLVEAGASLMKTDLQGDTPKHRAEKAKDAELAAYLENRQHYQMIQREDQETAV

Target ORF information:

RefSeq Version	XM_021477929.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477929.1

ORF Insert Sequence:

ATGTGGGCTC ATAGGAGGAA ATGGGTCTTG CCCCCCACCC CTCCGCATCA ACCGCACCAC 
AGGAAGGCTA TTGCCAAGTC CGGTCTCCAG CACCTCTCAT GCCAGCCCAG TGCTGCAGTG 
TTAGCCAAAG GGGAAACGGA CAGAGAAATA CGCAACACAG TAGACTGGAG TGAATCAGCT 
GTATACGGAG AGCACATCTG GTTTGAGACG AATGTCTCGG GGGACTTCTG TTACGTTGGA 
GAACAGCACT GTTACGCCAA GTCACTGCAA AAGTCTGTTG CTCGGAAAAA GTGTGCGGCT 
TGCAAGATCG TAGTTCACTC TATCTGCATT GAACAGCTGG AGAAGATAAA CTTCAGATGT 
AAACCGTCTT TCAGGGAGTC AGGATCACGG AACATCCGAG AGCAAACCTG GCGACGCAGA 
GCTTTCAGAG GTCGCTGTGC TTTGGAGCAA ATGAGAGGTG CTGCAGCTAA ATGTACTGCG 
CACACCTCTA ACACACAGCC TGCAATAGTG CGACACCACT GGGTCCATCG GAGAAGACAA 
GAGGGCAAAT GCAAACAGTG TGGAAAGGGG TTTCAGCAGA AATTTGCCTT TCACAGTAAA 
GAGATTGTAG CCATCAGCTG CTCCTGGTGC AAACAAGCAT ATCATAATAA GGTGACATGC 
TTTATGCTGC AGCAGATAGA AGAACCATGT TCATTAGGAG CTCATGCCGC TGTAATAGTA 
CCTCCTACTT GGATCATACG GGTTCGCCGA CCTCAGTCCT CACTAAAGTC AAGCAAAAAG 
AAAAAACGGA CATCTTTCAA ACGTAAATCC AGCAAGAAAG GAGCAGAGGA GGCACGATGG 
AAGCCTTTTA TAGTGAGACC CATTCCCTCC CAACTCATGA AGCCACTGCT AGTGTTTGTA 
AATCCAAAGA GTGGAGGAAA CCAGGGAGCT AAAATCATCC AGACCTTTCT TTGGTATCTA 
AACCCTCGGC AGGTGTTTGA TCTAAGTCAA GGAGGTCCTC AGGAAGGCTT GGAAATGTAT 
CGCAAGGTTC ACAACTTGAG AATTTTGGCC TGTGGGGGGG ATGGCACGGT GGGCTGGATC 
TTATCTGCAC TTGATCAGCT GCAATTAAAC CCTTCTCCTG CTGTGGCCGT ACTTCCCCTG 
GGCACAGGAA ATGACCTTGC TAGGACGCTA AACTGGGGTG GGGGCTACAC AGATGAACCT 
CTGAGTAAAA TCCTGTCTCA TGTGGAGGAT GGGAATATTG TACAGCTGGA TCGGTGGAAT 
CTTGTTGTGA AGCCCAACCC AGAGGCAGGA CCAGAGGAAA GAGACGAGCA GGTGACGGAC 
AAGCTCCCTC TAGACGTCTT CAATAACTAC TTCAGCCTTG GATTTGATGC TCATGTGACT 
TTAGAGTTCC ACGAATCTCG AGAGGCTAAT CCTGAGAAAT TTAACAGCCG GTTCAGAAAC 
AAAATGTTCT ATGCAGGGAC AGCGTTTTCT GATTTCTTGA TGGGCAGCTC CAAAGACCTG 
GCTAAGCACA TCAAAGTAGT CTGTGACGGC ACAGATTTAA CCTCAAAGGT CCAGGATTTG 
AAGCTGCAAT GCCTGGTCTT CCTAAATATC CCCAGGTACT GTGCGGGCAC AATGCCATGG 
GGCAATCCGA GTGAGCATCA TGACTTTGAG CCTCAGCGTC ATGATGATGG CTGCATTGAG 
GTTATTGGAT TCACAATGAC ATCACTGGCC ACACTGCAGG TCGGAGGTCA CGGTGAGAGG 
TTAAATCAGT GTCGCGAAGT CACCCTCACC ACCTTTAAGT CAATCCCCAT GCAGGTGGAC 
GGGGAGCCTT GTAAACTAGC ACCATCTGTT ATTCACATTT CTTTGCGAAA CCAGGCCAAC 
ATGGTGCAGA AAACCAAGAG ACGTACATCT ATACCACTGC TAAACGACAG TCAGCAACCG 
TTTCCAGAAC GACTACGGAT CAGAGTGAGC CGTATCAGCA TGCATGATTA TGAAGCTTTG 
CACTACGACA AGGAGAAGCT CAAAGAAGCT TCGATTCCAC TGGGTCTCAT CGTTGTCCCT 
GGAGACAGTG ATTTGGAAAC CTGCCGAGCT CACATCGAGA GACTACAGGA GGACTTTGTG 
TCGTGTAACC CGTCTCTGCA GCGGCTGGTG CTCCAGGAAG GTGACGGGAG CAAATCAAAG 
GCTTTGTCGT CTCAGAGGCT ATCACCGAAA TGGTGCTTTC TCGACTCTAC AACTGCTGAT 
CGATTCTACA GGATTGATCG AGCTCAAGAG CATCTCAACT ACGTGACTGA AATTTCTCAG 
GATGAGCTGT TTGTTTTGGA CCCAGAGCTG GTGGTCACAG AGACTGTTAG CACATCACCT 
GGCATGCCAG ACCTGGTGGA CTCTGGTGAA GTCCAGTCCG ATCCACACAA ATTTGCCTTT 
CCGTCATCCT CTTCTTCACC ACCAAACACA CCTGGACCTA GGGTTGCAGA GCTGCAAAGG 
AAGCGTGTTT CCAGTGACAG TTCGGTGGCA GAAGCACTTG CACACAGTGA CTCGCTCAGA 
TCAGCCAAGC CAGCTCTCTG CAGAGCTGGG GGTGTTCATC GCTCAAATAC AACCGCAGCA 
GATTTCAAAC CTACCAGCAG GTTTGAGAAG AGCGCTGTGA ACAATGCCAA CTCTGTGTCA 
GTGGACAGTC TAATTGAATG TGTGAAAAAG AAGGACCATC AGAAGTTGAA GGAGCTGCAT 
AAGAAAGGGG CCGACCTGTG TGTGCAGGAC CCTGCTGGAC GGACCCTATT GCACTATGCT 
GTGGAAGTGG GCAGCAAGGA GATTGTCAAA TACATCATTG ATAATGCACC CACCGATATC 
CTGGATGTAA CAGAGAAAGA AAATGGAGAG ACTGTGTTGC ACAAAGCAGC CTCGCTGTGT 
CATAGGACAA TCTGTCATTA CCTAGTGGAA GCAGGAGCAT CTCTTATGAA GACAGACTTA 
CAGGGTGACA CTCCGAAGCA TCGTGCCGAG AAGGCGAAAG ATGCTGAACT TGCGGCATAT 
CTTGAAAACC GCCAGCACTA CCAAATGATC CAGCGTGAGG ACCAGGAGAC TGCAGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dgkza ( XM_005169029.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005169086.1 Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X1, mRNA.

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CloneID	ODa13277
Clone ID Related Accession (Same CDS sequence)	XM_005169029.3 , XM_005169029.4
Accession Version	XM_005169029.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	diacylglycerol kinase zeta isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169029.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT 
TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG 
CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA 
GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT 
CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC 
CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC 
CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA 
TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA 
CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC 
CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT 
GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG 
ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC 
AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC 
CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA 
ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG 
ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG 
CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC 
ATTGAACAGC TGGAGAAGTT ATTGGATTGG AATGCTCCAC CTCAGGGGGA GGGGCTTGAT 
GAGATGCCTC TCAAAGAGCC GTCATTCAGA ATAAACTTCA GATGTAAACC GTCTTTCAGG 
GAGTCAGGAT CACGGAACAT CCGAGAGCCT GCAATAGTGC GACACCACTG GGTCCATCGG 
AGAAGACAAG AGGGCAAATG CAAACAGTGT GGAAAGGGGT TTCAGCAGAA ATTTGCCTTT 
CACAGTAAAG AGATTGTAGC CATCAGCTGC TCCTGGTGCA AACAAGCATA TCATAATAAG 
GTGACATGCT TTATGCTGCA GCAGATAGAA GAACCATGTT CATTAGGAGC TCATGCCGCT 
GTAATAGTAC CTCCTACTTG GATCATACGG GTTCGCCGAC CTCAGTCCTC ACTAAAGTCA 
AGCAAAAAGA AAAAACGGAC ATCTTTCAAA CGTAAATCCA GCAAGAAAGG AGCAGAGGAG 
GCACGATGGA AGCCTTTTAT AGTGAGACCC ATTCCCTCCC AACTCATGAA GCCACTGCTA 
GTGTTTGTAA ATCCAAAGAG TGGAGGAAAC CAGGGAGCTA AAATCATCCA GACCTTTCTT 
TGGTATCTAA ACCCTCGGCA GGTGTTTGAT CTAAGTCAAG GAGGTCCTCA GGAAGGCTTG 
GAAATGTATC GCAAGGTTCA CAACTTGAGA ATTTTGGCCT GTGGGGGGGA TGGCACGGTG 
GGCTGGATCT TATCTGCACT TGATCAGCTG CAATTAAACC CTTCTCCTGC TGTGGCCGTA 
CTTCCCCTGG GCACAGGAAA TGACCTTGCT AGGACGCTAA ACTGGGGTGG GGGCTACACA 
GATGAACCTC TGAGTAAAAT CCTGTCTCAT GTGGAGGATG GGAATATTGT ACAGCTGGAT 
CGGTGGAATC TTGTTGTGAA GCCCAACCCA GAGGCAGGAC CAGAGGAAAG AGACGAGCAG 
GTGACGGACA AGCTCCCTCT AGACGTCTTC AATAACTACT TCAGCCTTGG ATTTGATGCT 
CATGTGACTT TAGAGTTCCA CGAATCTCGA GAGGCTAATC CTGAGAAATT TAACAGCCGG 
TTCAGAAACA AAATGTTCTA TGCAGGGACA GCGTTTTCTG ATTTCTTGAT GGGCAGCTCC 
AAAGACCTGG CTAAGCACAT CAAAGTAGTC TGTGACGGCA CAGATTTAAC CTCAAAGGTC 
CAGGATTTGA AGCTGCAATG CCTGGTCTTC CTAAATATCC CCAGGTACTG TGCGGGCACA 
ATGCCATGGG GCAATCCGAG TGAGCATCAT GACTTTGAGC CTCAGCGTCA TGATGATGGC 
TGCATTGAGG TTATTGGATT CACAATGACA TCACTGGCCA CACTGCAGGT CGGAGGTCAC 
GGTGAGAGGT TAAATCAGTG TCGCGAAGTC ACCCTCACCA CCTTTAAGTC AATCCCCATG 
CAGGTGGACG GGGAGCCTTG TAAACTAGCA CCATCTGTTA TTCACATTTC TTTGCGAAAC 
CAGGCCAACA TGGTGCAGAA AACCAAGAGA CGTACATCTA TACCACTGCT AAACGACAGT 
CAGCAACCGT TTCCAGAACG ACTACGGATC AGAGTGAGCC GTATCAGCAT GCATGATTAT 
GAAGCTTTGC ACTACGACAA GGAGAAGCTC AAAGAAGCTT CGATTCCACT GGGTCTCATC 
GTTGTCCCTG GAGACAGTGA TTTGGAAACC TGCCGAGCTC ACATCGAGAG ACTACAGGAG 
GACTTTGTGT CGTGTAACCC GTCTCTGCAG CGGCTGGTGC TCCAGGAAGG TGACGGGAGC 
AAATCAAAGG CTTTGTCGTC TCAGAGGCTA TCACCGAAAT GGTGCTTTCT CGACTCTACA 
ACTGCTGATC GATTCTACAG GATTGATCGA GCTCAAGAGC ATCTCAACTA CGTGACTGAA 
ATTTCTCAGG ATGAGCTGTT TGTTTTGGAC CCAGAGCTGG TGGTCACAGA GACTGTTAGC 
ACATCACCTG GCATGCCAGA CCTGGTGGAC TCTGGTGAAG TCCAGTCCGA TCCACACAAA 
TTTGCCTTTC CGTCATCCTC TTCTTCACCA CCAAACACAC CTGGACCTAG GGTTGCAGAG 
CTGCAAAGGA AGCGTGTTTC CAGTGACAGT TCGGTGGCAG AAGCACTTGC ACACAGTGAC 
TCGCTCAGAT CAGCCAAGCC AGCTCTCTGC AGAGCTGGGG GTGTTCATCG CTCAAATACA 
ACCGCAGCAG ATTTCAAACC TACCAGCAGG TTTGAGAAGA GCGCTGTGAA CAATGCCAAC 
TCTGTGTCAG TGGACAGTCT AATTGAATGT GTGAAAAAGA AGGACCATCA GAAGTTGAAG 
GAGCTGCATA AGAAAGGGGC CGACCTGTGT GTGCAGGACC CTGCTGGACG GACCCTATTG 
CACTATGCTG TGGAAGTGGG CAGCAAGGAG ATTGTCAAAT ACATCATTGA TAATGCACCC 
ACCGATATCC TGGATGTAAC AGAGAAAGAA AATGGAGAGA CTGTGTTGCA CAAAGCAGCC 
TCGCTGTGTC ATAGGACAAT CTGTCATTAC CTAGTGGAAG CAGGAGCATC TCTTATGAAG 
ACAGACTTAC AGGGTGACAC TCCGAAGCAT CGTGCCGAGA AGGCGAAAGA TGCTGAACTT 
GCGGCATATC TTGAAAACCG CCAGCACTAC CAAATGATCC AGCGTGAGGA CCAGGAGACT 
GCAGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005169086.1
CDS	136..3924
Translation	MDALFNLRFRRKAVEPSGRRRSSVSLPASKARRRSSAGLPSAGLAHRRRSSAQLQIGSIQPGLVLHRRGHFTRRKSSGGTACLTPRFAIRRRQAAKHRSIHAQLLGPSLLLASVVQMSEDHEPEDKSAEEYSSLSDTDCSQDEHEQTGMDFETESDKCLTWLAQGLLVGESIQPRPLIRAPRCLRRNSSHLLPAEAVYRSPASYGLYGRYRRTSQVHNMGGLWAGKRSSLAARGSVALYRTSPPCWPDLSVSHTLQGTYYNPQIDTWSGFLSKAIAKSGLQHLSCQPSAAVLAKGETDREIRNTVDWSESAVYGEHIWFETNVSGDFCYVGEQHCYAKSLQKSVARKKCAACKIVVHSICIEQLEKLLDWNAPPQGEGLDEMPLKEPSFRINFRCKPSFRESGSRNIREPAIVRHHWVHRRRQEGKCKQCGKGFQQKFAFHSKEIVAISCSWCKQAYHNKVTCFMLQQIEEPCSLGAHAAVIVPPTWIIRVRRPQSSLKSSKKKKRTSFKRKSSKKGAEEARWKPFIVRPIPSQLMKPLLVFVNPKSGGNQGAKIIQTFLWYLNPRQVFDLSQGGPQEGLEMYRKVHNLRILACGGDGTVGWILSALDQLQLNPSPAVAVLPLGTGNDLARTLNWGGGYTDEPLSKILSHVEDGNIVQLDRWNLVVKPNPEAGPEERDEQVTDKLPLDVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGTAFSDFLMGSSKDLAKHIKVVCDGTDLTSKVQDLKLQCLVFLNIPRYCAGTMPWGNPSEHHDFEPQRHDDGCIEVIGFTMTSLATLQVGGHGERLNQCREVTLTTFKSIPMQVDGEPCKLAPSVIHISLRNQANMVQKTKRRTSIPLLNDSQQPFPERLRIRVSRISMHDYEALHYDKEKLKEASIPLGLIVVPGDSDLETCRAHIERLQEDFVSCNPSLQRLVLQEGDGSKSKALSSQRLSPKWCFLDSTTADRFYRIDRAQEHLNYVTEISQDELFVLDPELVVTETVSTSPGMPDLVDSGEVQSDPHKFAFPSSSSSPPNTPGPRVAELQRKRVSSDSSVAEALAHSDSLRSAKPALCRAGGVHRSNTTAADFKPTSRFEKSAVNNANSVSVDSLIECVKKKDHQKLKELHKKGADLCVQDPAGRTLLHYAVEVGSKEIVKYIIDNAPTDILDVTEKENGETVLHKAASLCHRTICHYLVEAGASLMKTDLQGDTPKHRAEKAKDAELAAYLENRQHYQMIQREDQETAV

Target ORF information:

RefSeq Version	XM_005169029.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005169029.3

ORF Insert Sequence:

ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT 
TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG 
CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA 
GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT 
CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC 
CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC 
CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA 
TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA 
CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC 
CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT 
GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG 
ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC 
AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC 
CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA 
ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG 
ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG 
CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC 
ATTGAACAGC TGGAGAAGTT ATTGGATTGG AATGCTCCAC CTCAGGGGGA GGGGCTTGAT 
GAGATGCCTC TCAAAGAGCC GTCATTCAGA ATAAACTTCA GATGTAAACC GTCTTTCAGG 
GAGTCAGGAT CACGGAACAT CCGAGAGCCT GCAATAGTGC GACACCACTG GGTCCATCGG 
AGAAGACAAG AGGGCAAATG CAAACAGTGT GGAAAGGGGT TTCAGCAGAA ATTTGCCTTT 
CACAGTAAAG AGATTGTAGC CATCAGCTGC TCCTGGTGCA AACAAGCATA TCATAATAAG 
GTGACATGCT TTATGCTGCA GCAGATAGAA GAACCATGTT CATTAGGAGC TCATGCCGCT 
GTAATAGTAC CTCCTACTTG GATCATACGG GTTCGCCGAC CTCAGTCCTC ACTAAAGTCA 
AGCAAAAAGA AAAAACGGAC ATCTTTCAAA CGTAAATCCA GCAAGAAAGG AGCAGAGGAG 
GCACGATGGA AGCCTTTTAT AGTGAGACCC ATTCCCTCCC AACTCATGAA GCCACTGCTA 
GTGTTTGTAA ATCCAAAGAG TGGAGGAAAC CAGGGAGCTA AAATCATCCA GACCTTTCTT 
TGGTATCTAA ACCCTCGGCA GGTGTTTGAT CTAAGTCAAG GAGGTCCTCA GGAAGGCTTG 
GAAATGTATC GCAAGGTTCA CAACTTGAGA ATTTTGGCCT GTGGGGGGGA TGGCACGGTG 
GGCTGGATCT TATCTGCACT TGATCAGCTG CAATTAAACC CTTCTCCTGC TGTGGCCGTA 
CTTCCCCTGG GCACAGGAAA TGACCTTGCT AGGACGCTAA ACTGGGGTGG GGGCTACACA 
GATGAACCTC TGAGTAAAAT CCTGTCTCAT GTGGAGGATG GGAATATTGT ACAGCTGGAT 
CGGTGGAATC TTGTTGTGAA GCCCAACCCA GAGGCAGGAC CAGAGGAAAG AGACGAGCAG 
GTGACGGACA AGCTCCCTCT AGACGTCTTC AATAACTACT TCAGCCTTGG ATTTGATGCT 
CATGTGACTT TAGAGTTCCA CGAATCTCGA GAGGCTAATC CTGAGAAATT TAACAGCCGG 
TTCAGAAACA AAATGTTCTA TGCAGGGACA GCGTTTTCTG ATTTCTTGAT GGGCAGCTCC 
AAAGACCTGG CTAAGCACAT CAAAGTAGTC TGTGACGGCA CAGATTTAAC CTCAAAGGTC 
CAGGATTTGA AGCTGCAATG CCTGGTCTTC CTAAATATCC CCAGGTACTG TGCGGGCACA 
ATGCCATGGG GCAATCCGAG TGAGCATCAT GACTTTGAGC CTCAGCGTCA TGATGATGGC 
TGCATTGAGG TTATTGGATT CACAATGACA TCACTGGCCA CACTGCAGGT CGGAGGTCAC 
GGTGAGAGGT TAAATCAGTG TCGCGAAGTC ACCCTCACCA CCTTTAAGTC AATCCCCATG 
CAGGTGGACG GGGAGCCTTG TAAACTAGCA CCATCTGTTA TTCACATTTC TTTGCGAAAC 
CAGGCCAACA TGGTGCAGAA AACCAAGAGA CGTACATCTA TACCACTGCT AAACGACAGT 
CAGCAACCGT TTCCAGAACG ACTACGGATC AGAGTGAGCC GTATCAGCAT GCATGATTAT 
GAAGCTTTGC ACTACGACAA GGAGAAGCTC AAAGAAGCTT CGATTCCACT GGGTCTCATC 
GTTGTCCCTG GAGACAGTGA TTTGGAAACC TGCCGAGCTC ACATCGAGAG ACTACAGGAG 
GACTTTGTGT CGTGTAACCC GTCTCTGCAG CGGCTGGTGC TCCAGGAAGG TGACGGGAGC 
AAATCAAAGG CTTTGTCGTC TCAGAGGCTA TCACCGAAAT GGTGCTTTCT CGACTCTACA 
ACTGCTGATC GATTCTACAG GATTGATCGA GCTCAAGAGC ATCTCAACTA CGTGACTGAA 
ATTTCTCAGG ATGAGCTGTT TGTTTTGGAC CCAGAGCTGG TGGTCACAGA GACTGTTAGC 
ACATCACCTG GCATGCCAGA CCTGGTGGAC TCTGGTGAAG TCCAGTCCGA TCCACACAAA 
TTTGCCTTTC CGTCATCCTC TTCTTCACCA CCAAACACAC CTGGACCTAG GGTTGCAGAG 
CTGCAAAGGA AGCGTGTTTC CAGTGACAGT TCGGTGGCAG AAGCACTTGC ACACAGTGAC 
TCGCTCAGAT CAGCCAAGCC AGCTCTCTGC AGAGCTGGGG GTGTTCATCG CTCAAATACA 
ACCGCAGCAG ATTTCAAACC TACCAGCAGG TTTGAGAAGA GCGCTGTGAA CAATGCCAAC 
TCTGTGTCAG TGGACAGTCT AATTGAATGT GTGAAAAAGA AGGACCATCA GAAGTTGAAG 
GAGCTGCATA AGAAAGGGGC CGACCTGTGT GTGCAGGACC CTGCTGGACG GACCCTATTG 
CACTATGCTG TGGAAGTGGG CAGCAAGGAG ATTGTCAAAT ACATCATTGA TAATGCACCC 
ACCGATATCC TGGATGTAAC AGAGAAAGAA AATGGAGAGA CTGTGTTGCA CAAAGCAGCC 
TCGCTGTGTC ATAGGACAAT CTGTCATTAC CTAGTGGAAG CAGGAGCATC TCTTATGAAG 
ACAGACTTAC AGGGTGACAC TCCGAAGCAT CGTGCCGAGA AGGCGAAAGA TGCTGAACTT 
GCGGCATATC TTGAAAACCG CCAGCACTAC CAAATGATCC AGCGTGAGGA CCAGGAGACT 
GCAGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dgkza ( XM_005169029.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005169086.1 Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa13277
Clone ID Related Accession (Same CDS sequence)	XM_005169029.3 , XM_005169029.4
Accession Version	XM_005169029.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	diacylglycerol kinase zeta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005169029.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT 
TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG 
CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA 
GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT 
CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC 
CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC 
CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA 
TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA 
CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC 
CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT 
GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG 
ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC 
AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC 
CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA 
ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG 
ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG 
CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC 
ATTGAACAGC TGGAGAAGTT ATTGGATTGG AATGCTCCAC CTCAGGGGGA GGGGCTTGAT 
GAGATGCCTC TCAAAGAGCC GTCATTCAGA ATAAACTTCA GATGTAAACC GTCTTTCAGG 
GAGTCAGGAT CACGGAACAT CCGAGAGCCT GCAATAGTGC GACACCACTG GGTCCATCGG 
AGAAGACAAG AGGGCAAATG CAAACAGTGT GGAAAGGGGT TTCAGCAGAA ATTTGCCTTT 
CACAGTAAAG AGATTGTAGC CATCAGCTGC TCCTGGTGCA AACAAGCATA TCATAATAAG 
GTGACATGCT TTATGCTGCA GCAGATAGAA GAACCATGTT CATTAGGAGC TCATGCCGCT 
GTAATAGTAC CTCCTACTTG GATCATACGG GTTCGCCGAC CTCAGTCCTC ACTAAAGTCA 
AGCAAAAAGA AAAAACGGAC ATCTTTCAAA CGTAAATCCA GCAAGAAAGG AGCAGAGGAG 
GCACGATGGA AGCCTTTTAT AGTGAGACCC ATTCCCTCCC AACTCATGAA GCCACTGCTA 
GTGTTTGTAA ATCCAAAGAG TGGAGGAAAC CAGGGAGCTA AAATCATCCA GACCTTTCTT 
TGGTATCTAA ACCCTCGGCA GGTGTTTGAT CTAAGTCAAG GAGGTCCTCA GGAAGGCTTG 
GAAATGTATC GCAAGGTTCA CAACTTGAGA ATTTTGGCCT GTGGGGGGGA TGGCACGGTG 
GGCTGGATCT TATCTGCACT TGATCAGCTG CAATTAAACC CTTCTCCTGC TGTGGCCGTA 
CTTCCCCTGG GCACAGGAAA TGACCTTGCT AGGACGCTAA ACTGGGGTGG GGGCTACACA 
GATGAACCTC TGAGTAAAAT CCTGTCTCAT GTGGAGGATG GGAATATTGT ACAGCTGGAT 
CGGTGGAATC TTGTTGTGAA GCCCAACCCA GAGGCAGGAC CAGAGGAAAG AGACGAGCAG 
GTGACGGACA AGCTCCCTCT AGACGTCTTC AATAACTACT TCAGCCTTGG ATTTGATGCT 
CATGTGACTT TAGAGTTCCA CGAATCTCGA GAGGCTAATC CTGAGAAATT TAACAGCCGG 
TTCAGAAACA AAATGTTCTA TGCAGGGACA GCGTTTTCTG ATTTCTTGAT GGGCAGCTCC 
AAAGACCTGG CTAAGCACAT CAAAGTAGTC TGTGACGGCA CAGATTTAAC CTCAAAGGTC 
CAGGATTTGA AGCTGCAATG CCTGGTCTTC CTAAATATCC CCAGGTACTG TGCGGGCACA 
ATGCCATGGG GCAATCCGAG TGAGCATCAT GACTTTGAGC CTCAGCGTCA TGATGATGGC 
TGCATTGAGG TTATTGGATT CACAATGACA TCACTGGCCA CACTGCAGGT CGGAGGTCAC 
GGTGAGAGGT TAAATCAGTG TCGCGAAGTC ACCCTCACCA CCTTTAAGTC AATCCCCATG 
CAGGTGGACG GGGAGCCTTG TAAACTAGCA CCATCTGTTA TTCACATTTC TTTGCGAAAC 
CAGGCCAACA TGGTGCAGAA AACCAAGAGA CGTACATCTA TACCACTGCT AAACGACAGT 
CAGCAACCGT TTCCAGAACG ACTACGGATC AGAGTGAGCC GTATCAGCAT GCATGATTAT 
GAAGCTTTGC ACTACGACAA GGAGAAGCTC AAAGAAGCTT CGATTCCACT GGGTCTCATC 
GTTGTCCCTG GAGACAGTGA TTTGGAAACC TGCCGAGCTC ACATCGAGAG ACTACAGGAG 
GACTTTGTGT CGTGTAACCC GTCTCTGCAG CGGCTGGTGC TCCAGGAAGG TGACGGGAGC 
AAATCAAAGG CTTTGTCGTC TCAGAGGCTA TCACCGAAAT GGTGCTTTCT CGACTCTACA 
ACTGCTGATC GATTCTACAG GATTGATCGA GCTCAAGAGC ATCTCAACTA CGTGACTGAA 
ATTTCTCAGG ATGAGCTGTT TGTTTTGGAC CCAGAGCTGG TGGTCACAGA GACTGTTAGC 
ACATCACCTG GCATGCCAGA CCTGGTGGAC TCTGGTGAAG TCCAGTCCGA TCCACACAAA 
TTTGCCTTTC CGTCATCCTC TTCTTCACCA CCAAACACAC CTGGACCTAG GGTTGCAGAG 
CTGCAAAGGA AGCGTGTTTC CAGTGACAGT TCGGTGGCAG AAGCACTTGC ACACAGTGAC 
TCGCTCAGAT CAGCCAAGCC AGCTCTCTGC AGAGCTGGGG GTGTTCATCG CTCAAATACA 
ACCGCAGCAG ATTTCAAACC TACCAGCAGG TTTGAGAAGA GCGCTGTGAA CAATGCCAAC 
TCTGTGTCAG TGGACAGTCT AATTGAATGT GTGAAAAAGA AGGACCATCA GAAGTTGAAG 
GAGCTGCATA AGAAAGGGGC CGACCTGTGT GTGCAGGACC CTGCTGGACG GACCCTATTG 
CACTATGCTG TGGAAGTGGG CAGCAAGGAG ATTGTCAAAT ACATCATTGA TAATGCACCC 
ACCGATATCC TGGATGTAAC AGAGAAAGAA AATGGAGAGA CTGTGTTGCA CAAAGCAGCC 
TCGCTGTGTC ATAGGACAAT CTGTCATTAC CTAGTGGAAG CAGGAGCATC TCTTATGAAG 
ACAGACTTAC AGGGTGACAC TCCGAAGCAT CGTGCCGAGA AGGCGAAAGA TGCTGAACTT 
GCGGCATATC TTGAAAACCG CCAGCACTAC CAAATGATCC AGCGTGAGGA CCAGGAGACT 
GCAGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005169086.1
CDS	171..3959
Translation	MDALFNLRFRRKAVEPSGRRRSSVSLPASKARRRSSAGLPSAGLAHRRRSSAQLQIGSIQPGLVLHRRGHFTRRKSSGGTACLTPRFAIRRRQAAKHRSIHAQLLGPSLLLASVVQMSEDHEPEDKSAEEYSSLSDTDCSQDEHEQTGMDFETESDKCLTWLAQGLLVGESIQPRPLIRAPRCLRRNSSHLLPAEAVYRSPASYGLYGRYRRTSQVHNMGGLWAGKRSSLAARGSVALYRTSPPCWPDLSVSHTLQGTYYNPQIDTWSGFLSKAIAKSGLQHLSCQPSAAVLAKGETDREIRNTVDWSESAVYGEHIWFETNVSGDFCYVGEQHCYAKSLQKSVARKKCAACKIVVHSICIEQLEKLLDWNAPPQGEGLDEMPLKEPSFRINFRCKPSFRESGSRNIREPAIVRHHWVHRRRQEGKCKQCGKGFQQKFAFHSKEIVAISCSWCKQAYHNKVTCFMLQQIEEPCSLGAHAAVIVPPTWIIRVRRPQSSLKSSKKKKRTSFKRKSSKKGAEEARWKPFIVRPIPSQLMKPLLVFVNPKSGGNQGAKIIQTFLWYLNPRQVFDLSQGGPQEGLEMYRKVHNLRILACGGDGTVGWILSALDQLQLNPSPAVAVLPLGTGNDLARTLNWGGGYTDEPLSKILSHVEDGNIVQLDRWNLVVKPNPEAGPEERDEQVTDKLPLDVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGTAFSDFLMGSSKDLAKHIKVVCDGTDLTSKVQDLKLQCLVFLNIPRYCAGTMPWGNPSEHHDFEPQRHDDGCIEVIGFTMTSLATLQVGGHGERLNQCREVTLTTFKSIPMQVDGEPCKLAPSVIHISLRNQANMVQKTKRRTSIPLLNDSQQPFPERLRIRVSRISMHDYEALHYDKEKLKEASIPLGLIVVPGDSDLETCRAHIERLQEDFVSCNPSLQRLVLQEGDGSKSKALSSQRLSPKWCFLDSTTADRFYRIDRAQEHLNYVTEISQDELFVLDPELVVTETVSTSPGMPDLVDSGEVQSDPHKFAFPSSSSSPPNTPGPRVAELQRKRVSSDSSVAEALAHSDSLRSAKPALCRAGGVHRSNTTAADFKPTSRFEKSAVNNANSVSVDSLIECVKKKDHQKLKELHKKGADLCVQDPAGRTLLHYAVEVGSKEIVKYIIDNAPTDILDVTEKENGETVLHKAASLCHRTICHYLVEAGASLMKTDLQGDTPKHRAEKAKDAELAAYLENRQHYQMIQREDQETAV

Target ORF information:

RefSeq Version	XM_005169029.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005169029.4

ORF Insert Sequence:

ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT 
TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG 
CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA 
GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT 
CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC 
CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC 
CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA 
TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA 
CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC 
CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT 
GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG 
ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC 
AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC 
CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA 
ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG 
ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG 
CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC 
ATTGAACAGC TGGAGAAGTT ATTGGATTGG AATGCTCCAC CTCAGGGGGA GGGGCTTGAT 
GAGATGCCTC TCAAAGAGCC GTCATTCAGA ATAAACTTCA GATGTAAACC GTCTTTCAGG 
GAGTCAGGAT CACGGAACAT CCGAGAGCCT GCAATAGTGC GACACCACTG GGTCCATCGG 
AGAAGACAAG AGGGCAAATG CAAACAGTGT GGAAAGGGGT TTCAGCAGAA ATTTGCCTTT 
CACAGTAAAG AGATTGTAGC CATCAGCTGC TCCTGGTGCA AACAAGCATA TCATAATAAG 
GTGACATGCT TTATGCTGCA GCAGATAGAA GAACCATGTT CATTAGGAGC TCATGCCGCT 
GTAATAGTAC CTCCTACTTG GATCATACGG GTTCGCCGAC CTCAGTCCTC ACTAAAGTCA 
AGCAAAAAGA AAAAACGGAC ATCTTTCAAA CGTAAATCCA GCAAGAAAGG AGCAGAGGAG 
GCACGATGGA AGCCTTTTAT AGTGAGACCC ATTCCCTCCC AACTCATGAA GCCACTGCTA 
GTGTTTGTAA ATCCAAAGAG TGGAGGAAAC CAGGGAGCTA AAATCATCCA GACCTTTCTT 
TGGTATCTAA ACCCTCGGCA GGTGTTTGAT CTAAGTCAAG GAGGTCCTCA GGAAGGCTTG 
GAAATGTATC GCAAGGTTCA CAACTTGAGA ATTTTGGCCT GTGGGGGGGA TGGCACGGTG 
GGCTGGATCT TATCTGCACT TGATCAGCTG CAATTAAACC CTTCTCCTGC TGTGGCCGTA 
CTTCCCCTGG GCACAGGAAA TGACCTTGCT AGGACGCTAA ACTGGGGTGG GGGCTACACA 
GATGAACCTC TGAGTAAAAT CCTGTCTCAT GTGGAGGATG GGAATATTGT ACAGCTGGAT 
CGGTGGAATC TTGTTGTGAA GCCCAACCCA GAGGCAGGAC CAGAGGAAAG AGACGAGCAG 
GTGACGGACA AGCTCCCTCT AGACGTCTTC AATAACTACT TCAGCCTTGG ATTTGATGCT 
CATGTGACTT TAGAGTTCCA CGAATCTCGA GAGGCTAATC CTGAGAAATT TAACAGCCGG 
TTCAGAAACA AAATGTTCTA TGCAGGGACA GCGTTTTCTG ATTTCTTGAT GGGCAGCTCC 
AAAGACCTGG CTAAGCACAT CAAAGTAGTC TGTGACGGCA CAGATTTAAC CTCAAAGGTC 
CAGGATTTGA AGCTGCAATG CCTGGTCTTC CTAAATATCC CCAGGTACTG TGCGGGCACA 
ATGCCATGGG GCAATCCGAG TGAGCATCAT GACTTTGAGC CTCAGCGTCA TGATGATGGC 
TGCATTGAGG TTATTGGATT CACAATGACA TCACTGGCCA CACTGCAGGT CGGAGGTCAC 
GGTGAGAGGT TAAATCAGTG TCGCGAAGTC ACCCTCACCA CCTTTAAGTC AATCCCCATG 
CAGGTGGACG GGGAGCCTTG TAAACTAGCA CCATCTGTTA TTCACATTTC TTTGCGAAAC 
CAGGCCAACA TGGTGCAGAA AACCAAGAGA CGTACATCTA TACCACTGCT AAACGACAGT 
CAGCAACCGT TTCCAGAACG ACTACGGATC AGAGTGAGCC GTATCAGCAT GCATGATTAT 
GAAGCTTTGC ACTACGACAA GGAGAAGCTC AAAGAAGCTT CGATTCCACT GGGTCTCATC 
GTTGTCCCTG GAGACAGTGA TTTGGAAACC TGCCGAGCTC ACATCGAGAG ACTACAGGAG 
GACTTTGTGT CGTGTAACCC GTCTCTGCAG CGGCTGGTGC TCCAGGAAGG TGACGGGAGC 
AAATCAAAGG CTTTGTCGTC TCAGAGGCTA TCACCGAAAT GGTGCTTTCT CGACTCTACA 
ACTGCTGATC GATTCTACAG GATTGATCGA GCTCAAGAGC ATCTCAACTA CGTGACTGAA 
ATTTCTCAGG ATGAGCTGTT TGTTTTGGAC CCAGAGCTGG TGGTCACAGA GACTGTTAGC 
ACATCACCTG GCATGCCAGA CCTGGTGGAC TCTGGTGAAG TCCAGTCCGA TCCACACAAA 
TTTGCCTTTC CGTCATCCTC TTCTTCACCA CCAAACACAC CTGGACCTAG GGTTGCAGAG 
CTGCAAAGGA AGCGTGTTTC CAGTGACAGT TCGGTGGCAG AAGCACTTGC ACACAGTGAC 
TCGCTCAGAT CAGCCAAGCC AGCTCTCTGC AGAGCTGGGG GTGTTCATCG CTCAAATACA 
ACCGCAGCAG ATTTCAAACC TACCAGCAGG TTTGAGAAGA GCGCTGTGAA CAATGCCAAC 
TCTGTGTCAG TGGACAGTCT AATTGAATGT GTGAAAAAGA AGGACCATCA GAAGTTGAAG 
GAGCTGCATA AGAAAGGGGC CGACCTGTGT GTGCAGGACC CTGCTGGACG GACCCTATTG 
CACTATGCTG TGGAAGTGGG CAGCAAGGAG ATTGTCAAAT ACATCATTGA TAATGCACCC 
ACCGATATCC TGGATGTAAC AGAGAAAGAA AATGGAGAGA CTGTGTTGCA CAAAGCAGCC 
TCGCTGTGTC ATAGGACAAT CTGTCATTAC CTAGTGGAAG CAGGAGCATC TCTTATGAAG 
ACAGACTTAC AGGGTGACAC TCCGAAGCAT CGTGCCGAGA AGGCGAAAGA TGCTGAACTT 
GCGGCATATC TTGAAAACCG CCAGCACTAC CAAATGATCC AGCGTGAGGA CCAGGAGACT 
GCAGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dgkza ( XM_005169030.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005169087.1 Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa13278
Clone ID Related Accession (Same CDS sequence)	XM_005169030.3
Accession Version	XM_005169030.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	diacylglycerol kinase zeta isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169030.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT 
TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG 
CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA 
GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT 
CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC 
CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC 
CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA 
TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA 
CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC 
CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT 
GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG 
ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC 
AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC 
CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA 
ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG 
ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG 
CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC 
ATTGAACAGC TGGAGAAGTT ATTGGATTGG AATGCTCCAC CTCAGGGGGA GGGGCTTGAT 
GAGATGCCTC TCAAAGAGCC GTCATTCAGA ATAAACTTCA GATGTAAACC GTCTTTCAGG 
GAGTCAGGAT CACGGAACAT CCGAGAGCCT GCAATAGTGC GACACCACTG GGTCCATCGG 
AGAAGACAAG AGGGCAAATG CAAACAGTGT GGAAAGGGGT TTCAGCAGAA ATTTGCCTTT 
CACAGTAAAG AGATTGTAGC CATCAGCTGC TCCTGGTGCA AACAAGCATA TCATAATAAG 
GTGACATGCT TTATGCTGCA GCAGATAGAA GAACCATGTT CATTAGGAGC TCATGCCGCT 
GTAATAGTAC CTCCTACTTG GATCATACGG GTTCGCCGAC CTCAGTCCTC ACTAAAGTCA 
AGCAAAAAGA AAAAACGGAC ATCTTTCAAA CGTAAATCCA GCAAGAAAGG AGCAGAGGAG 
GCACGATGGA AGCCTTTTAT AGTGAGACCC ATTCCCTCCC AACTCATGAA GCCACTGCTA 
GTGTTTGTAA ATCCAAAGAG TGGAGGAAAC CAGGGAGCTA AAATCATCCA GACCTTTCTT 
TGGTATCTAA ACCCTCGGCA GGTGTTTGAT CTAAGTCAAG GAGGTCCTCA GGAAGGCTTG 
GAAATGTATC GCAAGGTTCA CAACTTGAGA ATTTTGGCCT GTGGGGGGGA TGGCACGGTG 
GGCTGGATCT TATCTGCACT TGATCAGCTG CAATTAAACC CTTCTCCTGC TGTGGCCGTA 
CTTCCCCTGG GCACAGGAAA TGACCTTGCT AGGACGCTAA ACTGGGGTGG GGGCTACACA 
GATGAACCTC TGAGTAAAAT CCTGTCTCAT GTGGAGGATG GGAATATTGT ACAGCTGGAT 
CGGTGGAATC TTGTTGTGAA GCCCAACCCA GAGGCAGGAC CAGAGGAAAG AGACGAGCAG 
GTGACGGACA AGCTCCCTCT AGACGTCTTC AATAACTACT TCAGCCTTGG ATTTGATGCT 
CATGTGACTT TAGAGTTCCA CGAATCTCGA GAGGCTAATC CTGAGAAATT TAACAGCCGG 
TTCAGAAACA AAATGTTCTA TGCAGGGACA GCGTTTTCTG ATTTCTTGAT GGGCAGCTCC 
AAAGACCTGG CTAAGCACAT CAAAGTAGTC TGTGACGGCA CAGATTTAAC CTCAAAGGTC 
CAGGATTTGA AGCTGCAATG CCTGGTCTTC CTAAATATCC CCAGGTACTG TGCGGGCACA 
ATGCCATGGG GCAATCCGAG TGAGCATCAT GACTTTGAGC CTCAGCGTCA TGATGATGGC 
TGCATTGAGG TTATTGGATT CACAATGACA TCACTGGCCA CACTGCAGGT CGGAGGTCAC 
GGTGAGAGGT TAAATCAGTG TCGCGAAGTC ACCCTCACCA CCTTTAAGTC AATCCCCATG 
CAGGTGGACG GGGAGCCTTG TAAACTAGCA CCATCTGTTA TTCACATTTC TTTGCGAAAC 
CAGGCCAACA TGGTGCAGAA AACCAAGAGA CGTACATCTA TACCACTGCT AAACGACAGT 
CAGCAACCGT TTCCAGAACG ACTACGGATC AGAGTGAGCC GTATCAGCAT GCATGATTAT 
GAAGCTTTGC ACTACGACAA GGAGAAGCTC AAAGAAGCTT CGATTCCACT GGGTCTCATC 
GTTGTCCCTG GAGACAGTGA TTTGGAAACC TGCCGAGCTC ACATCGAGAG ACTACAGGAG 
GAAGGTGACG GGAGCAAATC AAAGGCTTTG TCGTCTCAGA GGCTATCACC GAAATGGTGC 
TTTCTCGACT CTACAACTGC TGATCGATTC TACAGGATTG ATCGAGCTCA AGAGCATCTC 
AACTACGTGA CTGAAATTTC TCAGGATGAG CTGTTTGTTT TGGACCCAGA GCTGGTGGTC 
ACAGAGACTG TTAGCACATC ACCTGGCATG CCAGACCTGG TGGACTCTGG TGAAGTCCAG 
TCCGATCCAC ACAAATTTGC CTTTCCGTCA TCCTCTTCTT CACCACCAAA CACACCTGGA 
CCTAGGGTTG CAGAGCTGCA AAGGAAGCGT GTTTCCAGTG ACAGTTCGGT GGCAGAAGCA 
CTTGCACACA GTGACTCGCT CAGATCAGCC AAGCCAGCTC TCTGCAGAGC TGGGGGTGTT 
CATCGCTCAA ATACAACCGC AGCAGATTTC AAACCTACCA GCAGGTTTGA GAAGAGCGCT 
GTGAACAATG CCAACTCTGT GTCAGTGGAC AGTCTAATTG AATGTGTGAA AAAGAAGGAC 
CATCAGAAGT TGAAGGAGCT GCATAAGAAA GGGGCCGACC TGTGTGTGCA GGACCCTGCT 
GGACGGACCC TATTGCACTA TGCTGTGGAA GTGGGCAGCA AGGAGATTGT CAAATACATC 
ATTGATAATG CACCCACCGA TATCCTGGAT GTAACAGAGA AAGAAAATGG AGAGACTGTG 
TTGCACAAAG CAGCCTCGCT GTGTCATAGG ACAATCTGTC ATTACCTAGT GGAAGCAGGA 
GCATCTCTTA TGAAGACAGA CTTACAGGGT GACACTCCGA AGCATCGTGC CGAGAAGGCG 
AAAGATGCTG AACTTGCGGC ATATCTTGAA AACCGCCAGC ACTACCAAAT GATCCAGCGT 
GAGGACCAGG AGACTGCAGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005169087.1
CDS	136..3879
Translation	MDALFNLRFRRKAVEPSGRRRSSVSLPASKARRRSSAGLPSAGLAHRRRSSAQLQIGSIQPGLVLHRRGHFTRRKSSGGTACLTPRFAIRRRQAAKHRSIHAQLLGPSLLLASVVQMSEDHEPEDKSAEEYSSLSDTDCSQDEHEQTGMDFETESDKCLTWLAQGLLVGESIQPRPLIRAPRCLRRNSSHLLPAEAVYRSPASYGLYGRYRRTSQVHNMGGLWAGKRSSLAARGSVALYRTSPPCWPDLSVSHTLQGTYYNPQIDTWSGFLSKAIAKSGLQHLSCQPSAAVLAKGETDREIRNTVDWSESAVYGEHIWFETNVSGDFCYVGEQHCYAKSLQKSVARKKCAACKIVVHSICIEQLEKLLDWNAPPQGEGLDEMPLKEPSFRINFRCKPSFRESGSRNIREPAIVRHHWVHRRRQEGKCKQCGKGFQQKFAFHSKEIVAISCSWCKQAYHNKVTCFMLQQIEEPCSLGAHAAVIVPPTWIIRVRRPQSSLKSSKKKKRTSFKRKSSKKGAEEARWKPFIVRPIPSQLMKPLLVFVNPKSGGNQGAKIIQTFLWYLNPRQVFDLSQGGPQEGLEMYRKVHNLRILACGGDGTVGWILSALDQLQLNPSPAVAVLPLGTGNDLARTLNWGGGYTDEPLSKILSHVEDGNIVQLDRWNLVVKPNPEAGPEERDEQVTDKLPLDVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGTAFSDFLMGSSKDLAKHIKVVCDGTDLTSKVQDLKLQCLVFLNIPRYCAGTMPWGNPSEHHDFEPQRHDDGCIEVIGFTMTSLATLQVGGHGERLNQCREVTLTTFKSIPMQVDGEPCKLAPSVIHISLRNQANMVQKTKRRTSIPLLNDSQQPFPERLRIRVSRISMHDYEALHYDKEKLKEASIPLGLIVVPGDSDLETCRAHIERLQEEGDGSKSKALSSQRLSPKWCFLDSTTADRFYRIDRAQEHLNYVTEISQDELFVLDPELVVTETVSTSPGMPDLVDSGEVQSDPHKFAFPSSSSSPPNTPGPRVAELQRKRVSSDSSVAEALAHSDSLRSAKPALCRAGGVHRSNTTAADFKPTSRFEKSAVNNANSVSVDSLIECVKKKDHQKLKELHKKGADLCVQDPAGRTLLHYAVEVGSKEIVKYIIDNAPTDILDVTEKENGETVLHKAASLCHRTICHYLVEAGASLMKTDLQGDTPKHRAEKAKDAELAAYLENRQHYQMIQREDQETAV

Target ORF information:

RefSeq Version	XM_005169030.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005169030.3

ORF Insert Sequence:

ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT 
TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG 
CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA 
GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT 
CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC 
CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC 
CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA 
TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA 
CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC 
CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT 
GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG 
ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC 
AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC 
CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA 
ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG 
ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG 
CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC 
ATTGAACAGC TGGAGAAGTT ATTGGATTGG AATGCTCCAC CTCAGGGGGA GGGGCTTGAT 
GAGATGCCTC TCAAAGAGCC GTCATTCAGA ATAAACTTCA GATGTAAACC GTCTTTCAGG 
GAGTCAGGAT CACGGAACAT CCGAGAGCCT GCAATAGTGC GACACCACTG GGTCCATCGG 
AGAAGACAAG AGGGCAAATG CAAACAGTGT GGAAAGGGGT TTCAGCAGAA ATTTGCCTTT 
CACAGTAAAG AGATTGTAGC CATCAGCTGC TCCTGGTGCA AACAAGCATA TCATAATAAG 
GTGACATGCT TTATGCTGCA GCAGATAGAA GAACCATGTT CATTAGGAGC TCATGCCGCT 
GTAATAGTAC CTCCTACTTG GATCATACGG GTTCGCCGAC CTCAGTCCTC ACTAAAGTCA 
AGCAAAAAGA AAAAACGGAC ATCTTTCAAA CGTAAATCCA GCAAGAAAGG AGCAGAGGAG 
GCACGATGGA AGCCTTTTAT AGTGAGACCC ATTCCCTCCC AACTCATGAA GCCACTGCTA 
GTGTTTGTAA ATCCAAAGAG TGGAGGAAAC CAGGGAGCTA AAATCATCCA GACCTTTCTT 
TGGTATCTAA ACCCTCGGCA GGTGTTTGAT CTAAGTCAAG GAGGTCCTCA GGAAGGCTTG 
GAAATGTATC GCAAGGTTCA CAACTTGAGA ATTTTGGCCT GTGGGGGGGA TGGCACGGTG 
GGCTGGATCT TATCTGCACT TGATCAGCTG CAATTAAACC CTTCTCCTGC TGTGGCCGTA 
CTTCCCCTGG GCACAGGAAA TGACCTTGCT AGGACGCTAA ACTGGGGTGG GGGCTACACA 
GATGAACCTC TGAGTAAAAT CCTGTCTCAT GTGGAGGATG GGAATATTGT ACAGCTGGAT 
CGGTGGAATC TTGTTGTGAA GCCCAACCCA GAGGCAGGAC CAGAGGAAAG AGACGAGCAG 
GTGACGGACA AGCTCCCTCT AGACGTCTTC AATAACTACT TCAGCCTTGG ATTTGATGCT 
CATGTGACTT TAGAGTTCCA CGAATCTCGA GAGGCTAATC CTGAGAAATT TAACAGCCGG 
TTCAGAAACA AAATGTTCTA TGCAGGGACA GCGTTTTCTG ATTTCTTGAT GGGCAGCTCC 
AAAGACCTGG CTAAGCACAT CAAAGTAGTC TGTGACGGCA CAGATTTAAC CTCAAAGGTC 
CAGGATTTGA AGCTGCAATG CCTGGTCTTC CTAAATATCC CCAGGTACTG TGCGGGCACA 
ATGCCATGGG GCAATCCGAG TGAGCATCAT GACTTTGAGC CTCAGCGTCA TGATGATGGC 
TGCATTGAGG TTATTGGATT CACAATGACA TCACTGGCCA CACTGCAGGT CGGAGGTCAC 
GGTGAGAGGT TAAATCAGTG TCGCGAAGTC ACCCTCACCA CCTTTAAGTC AATCCCCATG 
CAGGTGGACG GGGAGCCTTG TAAACTAGCA CCATCTGTTA TTCACATTTC TTTGCGAAAC 
CAGGCCAACA TGGTGCAGAA AACCAAGAGA CGTACATCTA TACCACTGCT AAACGACAGT 
CAGCAACCGT TTCCAGAACG ACTACGGATC AGAGTGAGCC GTATCAGCAT GCATGATTAT 
GAAGCTTTGC ACTACGACAA GGAGAAGCTC AAAGAAGCTT CGATTCCACT GGGTCTCATC 
GTTGTCCCTG GAGACAGTGA TTTGGAAACC TGCCGAGCTC ACATCGAGAG ACTACAGGAG 
GAAGGTGACG GGAGCAAATC AAAGGCTTTG TCGTCTCAGA GGCTATCACC GAAATGGTGC 
TTTCTCGACT CTACAACTGC TGATCGATTC TACAGGATTG ATCGAGCTCA AGAGCATCTC 
AACTACGTGA CTGAAATTTC TCAGGATGAG CTGTTTGTTT TGGACCCAGA GCTGGTGGTC 
ACAGAGACTG TTAGCACATC ACCTGGCATG CCAGACCTGG TGGACTCTGG TGAAGTCCAG 
TCCGATCCAC ACAAATTTGC CTTTCCGTCA TCCTCTTCTT CACCACCAAA CACACCTGGA 
CCTAGGGTTG CAGAGCTGCA AAGGAAGCGT GTTTCCAGTG ACAGTTCGGT GGCAGAAGCA 
CTTGCACACA GTGACTCGCT CAGATCAGCC AAGCCAGCTC TCTGCAGAGC TGGGGGTGTT 
CATCGCTCAA ATACAACCGC AGCAGATTTC AAACCTACCA GCAGGTTTGA GAAGAGCGCT 
GTGAACAATG CCAACTCTGT GTCAGTGGAC AGTCTAATTG AATGTGTGAA AAAGAAGGAC 
CATCAGAAGT TGAAGGAGCT GCATAAGAAA GGGGCCGACC TGTGTGTGCA GGACCCTGCT 
GGACGGACCC TATTGCACTA TGCTGTGGAA GTGGGCAGCA AGGAGATTGT CAAATACATC 
ATTGATAATG CACCCACCGA TATCCTGGAT GTAACAGAGA AAGAAAATGG AGAGACTGTG 
TTGCACAAAG CAGCCTCGCT GTGTCATAGG ACAATCTGTC ATTACCTAGT GGAAGCAGGA 
GCATCTCTTA TGAAGACAGA CTTACAGGGT GACACTCCGA AGCATCGTGC CGAGAAGGCG 
AAAGATGCTG AACTTGCGGC ATATCTTGAA AACCGCCAGC ACTACCAAAT GATCCAGCGT 
GAGGACCAGG AGACTGCAGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dgkza ( XM_005169031.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005169088.1 Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X4, mRNA.

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CloneID	ODa13279
Clone ID Related Accession (Same CDS sequence)	XM_005169031.3
Accession Version	XM_005169031.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	diacylglycerol kinase zeta isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169031.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT 
TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG 
CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA 
GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT 
CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC 
CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC 
CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA 
TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA 
CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC 
CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT 
GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG 
ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC 
AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC 
CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA 
ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG 
ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG 
CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC 
ATTGAACAGC TGGAGAAGTT ATTGGATTGG AATGCTCCAC CTCAGGGGGA GGGGCTTGAT 
GAGATGCCTC TCAAAGAGCC GTCATTCAGA ATAAACTTCA GATGTAAACC GTCTTTCAGG 
GAGTCAGGAT CACGGAACAT CCGAGAGCCT GCAATAGTGC GACACCACTG GGTCCATCGG 
AGAAGACAAG AGGGCAAATG CAAACAGTGT GGAAAGGGGT TTCAGCAGAA ATTTGCCTTT 
CACAGTAAAG AGATTGTAGC CATCAGCTGC TCCTGGTGCA AACAAGCATA TCATAATAAG 
GTGACATGCT TTATGCTGCA GCAGATAGAA GAACCATGTT CATTAGGAGC TCATGCCGCT 
GTAATAGTAC CTCCTACTTG GATCATACGG GTTCGCCGAC CTCAGTCCTC ACTAAAGTCA 
AGCAAAAAGA AAAAACGGAC ATCTTTCAAA CGTAAATCCA GCAAGAAAGG AGCAGAGGAG 
GCACGATGGA AGCCTTTTAT AGTGAGACCC ATTCCCTCCC AACTCATGAA GCCACTGCTA 
GTGTTTGTAA ATCCAAAGAG TGGAGGAAAC CAGGGAGCTA AAATCATCCA GACCTTTCTT 
TGGTATCTAA ACCCTCGGCA GGTGTTTGAT CTAAGTCAAG GAGGTCCTCA GGAAGGCTTG 
GAAATGTATC GCAAGGTTCA CAACTTGAGA ATTTTGGCCT GTGGGGGGGA TGGCACGGTG 
GGCTGGATCT TATCTGCACT TGATCAGCTG CAATTAAACC CTTCTCCTGC TGTGGCCGTA 
CTTCCCCTGG GCACAGGAAA TGACCTTGCT AGGACGCTAA ACTGGGGTGG GGGCTACACA 
GATGAACCTC TGAGTAAAAT CCTGTCTCAT GTGGAGGATG GGAATATTGT ACAGCTGGAT 
CGGTGGAATC TTGTTGTGAA GCCCAACCCA GAGGCAGGAC CAGAGGAAAG AGACGAGCAG 
GTGACGGACA AGCTCCCTCT AGACGTCTTC AATAACTACT TCAGCCTTGG ATTTGATGCT 
CATGTGACTT TAGAGTTCCA CGAATCTCGA GAGGCTAATC CTGAGAAATT TAACAGCCGG 
TTCAGAAACA AAATGTTCTA TGCAGGGACA GCGTTTTCTG ATTTCTTGAT GGGCAGCTCC 
AAAGACCTGG CTAAGCACAT CAAAGTAGTC TGTGACGGCA CAGATTTAAC CTCAAAGGTC 
CAGGATTTGA AGCTGCAATG CCTGGTCTTC CTAAATATCC CCAGGTACTG TGCGGGCACA 
ATGCCATGGG GCAATCCGAG TGAGCATCAT GACTTTGAGC CTCAGCGTCA TGATGATGGC 
TGCATTGAGG TTATTGGATT CACAATGACA TCACTGGCCA CACTGCAGGT CGGAGGTCAC 
GGTGAGAGGT TAAATCAGTG TCGCGAAGTC ACCCTCACCA CCTTTAAGTC AATCCCCATG 
CAGGTGGACG GGGAGCCTTG TAAACTAGCA CCATCTGTTA TTCACATTTC TTTGCGAAAC 
CAGGCCAACA TGGTGCAGAA AACCAAGAGA CGTACATCTA TACCACTGCT AAACGATCAG 
CAACCGTTTC CAGAACGACT ACGGATCAGA GTGAGCCGTA TCAGCATGCA TGATTATGAA 
GCTTTGCACT ACGACAAGGA GAAGCTCAAA GAAGCTTCGA TTCCACTGGG TCTCATCGTT 
GTCCCTGGAG ACAGTGATTT GGAAACCTGC CGAGCTCACA TCGAGAGACT ACAGGAGGAA 
GGTGACGGGA GCAAATCAAA GGCTTTGTCG TCTCAGAGGC TATCACCGAA ATGGTGCTTT 
CTCGACTCTA CAACTGCTGA TCGATTCTAC AGGATTGATC GAGCTCAAGA GCATCTCAAC 
TACGTGACTG AAATTTCTCA GGATGAGCTG TTTGTTTTGG ACCCAGAGCT GGTGGTCACA 
GAGACTGTTA GCACATCACC TGGCATGCCA GACCTGGTGG ACTCTGGTGA AGTCCAGTCC 
GATCCACACA AATTTGCCTT TCCGTCATCC TCTTCTTCAC CACCAAACAC ACCTGGACCT 
AGGGTTGCAG AGCTGCAAAG GAAGCGTGTT TCCAGTGACA GTTCGGTGGC AGAAGCACTT 
GCACACAGTG ACTCGCTCAG ATCAGCCAAG CCAGCTCTCT GCAGAGCTGG GGGTGTTCAT 
CGCTCAAATA CAACCGCAGC AGATTTCAAA CCTACCAGCA GGTTTGAGAA GAGCGCTGTG 
AACAATGCCA ACTCTGTGTC AGTGGACAGT CTAATTGAAT GTGTGAAAAA GAAGGACCAT 
CAGAAGTTGA AGGAGCTGCA TAAGAAAGGG GCCGACCTGT GTGTGCAGGA CCCTGCTGGA 
CGGACCCTAT TGCACTATGC TGTGGAAGTG GGCAGCAAGG AGATTGTCAA ATACATCATT 
GATAATGCAC CCACCGATAT CCTGGATGTA ACAGAGAAAG AAAATGGAGA GACTGTGTTG 
CACAAAGCAG CCTCGCTGTG TCATAGGACA ATCTGTCATT ACCTAGTGGA AGCAGGAGCA 
TCTCTTATGA AGACAGACTT ACAGGGTGAC ACTCCGAAGC ATCGTGCCGA GAAGGCGAAA 
GATGCTGAAC TTGCGGCATA TCTTGAAAAC CGCCAGCACT ACCAAATGAT CCAGCGTGAG 
GACCAGGAGA CTGCAGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005169088.1
CDS	136..3876
Translation	MDALFNLRFRRKAVEPSGRRRSSVSLPASKARRRSSAGLPSAGLAHRRRSSAQLQIGSIQPGLVLHRRGHFTRRKSSGGTACLTPRFAIRRRQAAKHRSIHAQLLGPSLLLASVVQMSEDHEPEDKSAEEYSSLSDTDCSQDEHEQTGMDFETESDKCLTWLAQGLLVGESIQPRPLIRAPRCLRRNSSHLLPAEAVYRSPASYGLYGRYRRTSQVHNMGGLWAGKRSSLAARGSVALYRTSPPCWPDLSVSHTLQGTYYNPQIDTWSGFLSKAIAKSGLQHLSCQPSAAVLAKGETDREIRNTVDWSESAVYGEHIWFETNVSGDFCYVGEQHCYAKSLQKSVARKKCAACKIVVHSICIEQLEKLLDWNAPPQGEGLDEMPLKEPSFRINFRCKPSFRESGSRNIREPAIVRHHWVHRRRQEGKCKQCGKGFQQKFAFHSKEIVAISCSWCKQAYHNKVTCFMLQQIEEPCSLGAHAAVIVPPTWIIRVRRPQSSLKSSKKKKRTSFKRKSSKKGAEEARWKPFIVRPIPSQLMKPLLVFVNPKSGGNQGAKIIQTFLWYLNPRQVFDLSQGGPQEGLEMYRKVHNLRILACGGDGTVGWILSALDQLQLNPSPAVAVLPLGTGNDLARTLNWGGGYTDEPLSKILSHVEDGNIVQLDRWNLVVKPNPEAGPEERDEQVTDKLPLDVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGTAFSDFLMGSSKDLAKHIKVVCDGTDLTSKVQDLKLQCLVFLNIPRYCAGTMPWGNPSEHHDFEPQRHDDGCIEVIGFTMTSLATLQVGGHGERLNQCREVTLTTFKSIPMQVDGEPCKLAPSVIHISLRNQANMVQKTKRRTSIPLLNDQQPFPERLRIRVSRISMHDYEALHYDKEKLKEASIPLGLIVVPGDSDLETCRAHIERLQEEGDGSKSKALSSQRLSPKWCFLDSTTADRFYRIDRAQEHLNYVTEISQDELFVLDPELVVTETVSTSPGMPDLVDSGEVQSDPHKFAFPSSSSSPPNTPGPRVAELQRKRVSSDSSVAEALAHSDSLRSAKPALCRAGGVHRSNTTAADFKPTSRFEKSAVNNANSVSVDSLIECVKKKDHQKLKELHKKGADLCVQDPAGRTLLHYAVEVGSKEIVKYIIDNAPTDILDVTEKENGETVLHKAASLCHRTICHYLVEAGASLMKTDLQGDTPKHRAEKAKDAELAAYLENRQHYQMIQREDQETAV

Target ORF information:

RefSeq Version	XM_005169031.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005169031.3

ORF Insert Sequence:

ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT 
TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG 
CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA 
GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT 
CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC 
CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC 
CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA 
TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA 
CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC 
CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT 
GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG 
ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC 
AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC 
CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA 
ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG 
ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG 
CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC 
ATTGAACAGC TGGAGAAGTT ATTGGATTGG AATGCTCCAC CTCAGGGGGA GGGGCTTGAT 
GAGATGCCTC TCAAAGAGCC GTCATTCAGA ATAAACTTCA GATGTAAACC GTCTTTCAGG 
GAGTCAGGAT CACGGAACAT CCGAGAGCCT GCAATAGTGC GACACCACTG GGTCCATCGG 
AGAAGACAAG AGGGCAAATG CAAACAGTGT GGAAAGGGGT TTCAGCAGAA ATTTGCCTTT 
CACAGTAAAG AGATTGTAGC CATCAGCTGC TCCTGGTGCA AACAAGCATA TCATAATAAG 
GTGACATGCT TTATGCTGCA GCAGATAGAA GAACCATGTT CATTAGGAGC TCATGCCGCT 
GTAATAGTAC CTCCTACTTG GATCATACGG GTTCGCCGAC CTCAGTCCTC ACTAAAGTCA 
AGCAAAAAGA AAAAACGGAC ATCTTTCAAA CGTAAATCCA GCAAGAAAGG AGCAGAGGAG 
GCACGATGGA AGCCTTTTAT AGTGAGACCC ATTCCCTCCC AACTCATGAA GCCACTGCTA 
GTGTTTGTAA ATCCAAAGAG TGGAGGAAAC CAGGGAGCTA AAATCATCCA GACCTTTCTT 
TGGTATCTAA ACCCTCGGCA GGTGTTTGAT CTAAGTCAAG GAGGTCCTCA GGAAGGCTTG 
GAAATGTATC GCAAGGTTCA CAACTTGAGA ATTTTGGCCT GTGGGGGGGA TGGCACGGTG 
GGCTGGATCT TATCTGCACT TGATCAGCTG CAATTAAACC CTTCTCCTGC TGTGGCCGTA 
CTTCCCCTGG GCACAGGAAA TGACCTTGCT AGGACGCTAA ACTGGGGTGG GGGCTACACA 
GATGAACCTC TGAGTAAAAT CCTGTCTCAT GTGGAGGATG GGAATATTGT ACAGCTGGAT 
CGGTGGAATC TTGTTGTGAA GCCCAACCCA GAGGCAGGAC CAGAGGAAAG AGACGAGCAG 
GTGACGGACA AGCTCCCTCT AGACGTCTTC AATAACTACT TCAGCCTTGG ATTTGATGCT 
CATGTGACTT TAGAGTTCCA CGAATCTCGA GAGGCTAATC CTGAGAAATT TAACAGCCGG 
TTCAGAAACA AAATGTTCTA TGCAGGGACA GCGTTTTCTG ATTTCTTGAT GGGCAGCTCC 
AAAGACCTGG CTAAGCACAT CAAAGTAGTC TGTGACGGCA CAGATTTAAC CTCAAAGGTC 
CAGGATTTGA AGCTGCAATG CCTGGTCTTC CTAAATATCC CCAGGTACTG TGCGGGCACA 
ATGCCATGGG GCAATCCGAG TGAGCATCAT GACTTTGAGC CTCAGCGTCA TGATGATGGC 
TGCATTGAGG TTATTGGATT CACAATGACA TCACTGGCCA CACTGCAGGT CGGAGGTCAC 
GGTGAGAGGT TAAATCAGTG TCGCGAAGTC ACCCTCACCA CCTTTAAGTC AATCCCCATG 
CAGGTGGACG GGGAGCCTTG TAAACTAGCA CCATCTGTTA TTCACATTTC TTTGCGAAAC 
CAGGCCAACA TGGTGCAGAA AACCAAGAGA CGTACATCTA TACCACTGCT AAACGATCAG 
CAACCGTTTC CAGAACGACT ACGGATCAGA GTGAGCCGTA TCAGCATGCA TGATTATGAA 
GCTTTGCACT ACGACAAGGA GAAGCTCAAA GAAGCTTCGA TTCCACTGGG TCTCATCGTT 
GTCCCTGGAG ACAGTGATTT GGAAACCTGC CGAGCTCACA TCGAGAGACT ACAGGAGGAA 
GGTGACGGGA GCAAATCAAA GGCTTTGTCG TCTCAGAGGC TATCACCGAA ATGGTGCTTT 
CTCGACTCTA CAACTGCTGA TCGATTCTAC AGGATTGATC GAGCTCAAGA GCATCTCAAC 
TACGTGACTG AAATTTCTCA GGATGAGCTG TTTGTTTTGG ACCCAGAGCT GGTGGTCACA 
GAGACTGTTA GCACATCACC TGGCATGCCA GACCTGGTGG ACTCTGGTGA AGTCCAGTCC 
GATCCACACA AATTTGCCTT TCCGTCATCC TCTTCTTCAC CACCAAACAC ACCTGGACCT 
AGGGTTGCAG AGCTGCAAAG GAAGCGTGTT TCCAGTGACA GTTCGGTGGC AGAAGCACTT 
GCACACAGTG ACTCGCTCAG ATCAGCCAAG CCAGCTCTCT GCAGAGCTGG GGGTGTTCAT 
CGCTCAAATA CAACCGCAGC AGATTTCAAA CCTACCAGCA GGTTTGAGAA GAGCGCTGTG 
AACAATGCCA ACTCTGTGTC AGTGGACAGT CTAATTGAAT GTGTGAAAAA GAAGGACCAT 
CAGAAGTTGA AGGAGCTGCA TAAGAAAGGG GCCGACCTGT GTGTGCAGGA CCCTGCTGGA 
CGGACCCTAT TGCACTATGC TGTGGAAGTG GGCAGCAAGG AGATTGTCAA ATACATCATT 
GATAATGCAC CCACCGATAT CCTGGATGTA ACAGAGAAAG AAAATGGAGA GACTGTGTTG 
CACAAAGCAG CCTCGCTGTG TCATAGGACA ATCTGTCATT ACCTAGTGGA AGCAGGAGCA 
TCTCTTATGA AGACAGACTT ACAGGGTGAC ACTCCGAAGC ATCGTGCCGA GAAGGCGAAA 
GATGCTGAAC TTGCGGCATA TCTTGAAAAC CGCCAGCACT ACCAAATGAT CCAGCGTGAG 
GACCAGGAGA CTGCAGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dgkza ( XM_005169032.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005169089.1 Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa13280
Clone ID Related Accession (Same CDS sequence)	XM_005169032.3
Accession Version	XM_005169032.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	diacylglycerol kinase zeta isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169032.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT 
TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG 
CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA 
GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT 
CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC 
CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC 
CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA 
TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA 
CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC 
CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT 
GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG 
ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC 
AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC 
CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA 
ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG 
ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG 
CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC 
ATTGAACAGC TGGAGAAGTT ATTGGATTGG AATGCTCCAC CTCAGGGGGA GGGGCTTGAT 
GAGATGCCTC TCAAAGAGCC GTCATTCAGA ATAAACTTCA GATGTAAACC GTCTTTCAGG 
GAGTCAGGAT CACGGAACAT CCGAGAGCCT GCAATAGTGC GACACCACTG GGTCCATCGG 
AGAAGACAAG AGGGCAAATG CAAACAGTGT GGAAAGGGGT TTCAGCAGAA ATTTGCCTTT 
CACAGTAAAG AGATTGTAGC CATCAGCTGC TCCTGGTGCA AACAAGCATA TCATAATAAG 
GTGACATGCT TTATGCTGCA GCAGATAGAA GAACCATGTT CATTAGGAGC TCATGCCGCT 
GTAATAGTAC CTCCTACTTG GATCATACGG GTTCGCCGAC CTCAGTCCTC ACTAAAGTCA 
AGCAAAAAGA AAAAACGGAC ATCTTTCAAA CGTAAATCCA GCAAGAAAGG AGCAGAGGAG 
GCACGATGGA AGCCTTTTAT AGTGAGACCC ATTCCCTCCC AACTCATGAA GCCACTGCTA 
GTGTTTGTAA ATCCAAAGAG TGGAGGAAAC CAGGGAGCTA AAATCATCCA GACCTTTCTT 
TGGTATCTAA ACCCTCGGCA GGTGTTTGAT CTAAGTCAAG GAGGTCCTCA GGAAGGCTTG 
GAAATGTATC GCAAGGTTCA CAACTTGAGA ATTTTGGCCT GTGGGGGGGA TGGCACGGTG 
GGCTGGATCT TATCTGCACT TGATCAGCTG CAATTAAACC CTTCTCCTGC TGTGGCCGTA 
CTTCCCCTGG GCACAGGAAA TGACCTTGCT AGGACGCTAA ACTGGGGTGG GGGCTACACA 
GATGAACCTC TGAGTAAAAT CCTGTCTCAT GTGGAGGATG GGAATATTGT ACAGCTGGAT 
CGGTGGAATC TTGTTGTGAA GCCCAACCCA GAGGCAGGAC CAGAGGAAAG AGACGAGCAG 
GTGACGGACA AGCTCCCTCT AGACGTCTTC AATAACTACT TCAGCCTTGG ATTTGATGCT 
CATGTGACTT TAGAGTTCCA CGAATCTCGA GAGGCTAATC CTGAGAAATT TAACAGCCGG 
TTCAGAAACA AAATGTTCTA TGCAGGGACA GCGTTTTCTG ATTTCTTGAT GGGCAGCTCC 
AAAGACCTGG CTAAGCACAT CAAAGTAGTC TGTGACGGCA CAGATTTAAC CTCAAAGGTC 
CAGGATTTGA AGCTGCAATG CCTGGTCTTC CTAAATATCC CCAGGTACTG TGCGGGCACA 
ATGCCATGGG GCAATCCGAG TGAGCATCAT GACTTTGAGC CTCAGCGTCA TGATGATGGC 
TGCATTGAGG TTATTGGATT CACAATGACA TCACTGGCCA CACTGCAGGT CGGAGGTCAC 
GGTGAGAGGT TAAATCAGTG TCGCGAAGTC ACCCTCACCA CCTTTAAGTC AATCCCCATG 
CAGGTGGACG GGGAGCCTTG TAAACTAGCA CCATCTGTTA TTCACATTTC TTTGCGAAAC 
CAGGCCAACA TGGTGCAGAA AACCAAGAGA CGTACATCTA TACCACTGCT AAACGACAGT 
CAGCAACCGT TTCCAGAACG ACTACGGATC AGAGTGAGCC GTATCAGCAT GCATGATTAT 
GAAGCTTTGC ACTACGACAA GGAGAAGCTC AAAGAAGCTT CGATTCCACT GGGTCTCATC 
GTTGTCCCTG GAGACAGTGA TTTGGAAACC TGCCGAGCTC ACATCGAGAG ACTACAGGAG 
GACTTTGTGT CGTGTAACCC GTCTCTGCAG CGGCTGGTGC TCCAGGAAGG TGACGGGAGC 
AAATCAAAGG CTTTGTCGTC TCAGAGGCTA TCACCGAAAT GGTGCTTTCT CGACTCTACA 
ACTGCTGATC GATTCTACAG GATTGATCGA GCTCAAGAGC ATCTCAACTA CGTGACTGAA 
ATTTCTCAGG ATGAGCTGTT TGTTTTGGAC CCAGAGCTGG TGGTCACAGA GACTGTTAGC 
ACATCACCTG GCATGCCAGA CCTGGTGGAC TCTGGTGAAG TCCAGTCCGA TCCACACAAA 
TTTGCCTTTC CGTCATCCTC TTCTTCACCA CCAAACACAC CTGGACCTAG GGTTGCAGAG 
CTGCAAAGGA AGCGTGTTTC CAGTGACAGT TCGGTGGCAG AAGCACTTGC ACACAGTGAC 
TCGCTCAGAT CAGCCAAGCC AGCTCTCTGC AGGTTTGAGA AGAGCGCTGT GAACAATGCC 
AACTCTGTGT CAGTGGACAG TCTAATTGAA TGTGTGAAAA AGAAGGACCA TCAGAAGTTG 
AAGGAGCTGC ATAAGAAAGG GGCCGACCTG TGTGTGCAGG ACCCTGCTGG ACGGACCCTA 
TTGCACTATG CTGTGGAAGT GGGCAGCAAG GAGATTGTCA AATACATCAT TGATAATGCA 
CCCACCGATA TCCTGGATGT AACAGAGAAA GAAAATGGAG AGACTGTGTT GCACAAAGCA 
GCCTCGCTGT GTCATAGGAC AATCTGTCAT TACCTAGTGG AAGCAGGAGC ATCTCTTATG 
AAGACAGACT TACAGGGTGA CACTCCGAAG CATCGTGCCG AGAAGGCGAA AGATGCTGAA 
CTTGCGGCAT ATCTTGAAAA CCGCCAGCAC TACCAAATGA TCCAGCGTGA GGACCAGGAG 
ACTGCAGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005169089.1
CDS	136..3867
Translation	MDALFNLRFRRKAVEPSGRRRSSVSLPASKARRRSSAGLPSAGLAHRRRSSAQLQIGSIQPGLVLHRRGHFTRRKSSGGTACLTPRFAIRRRQAAKHRSIHAQLLGPSLLLASVVQMSEDHEPEDKSAEEYSSLSDTDCSQDEHEQTGMDFETESDKCLTWLAQGLLVGESIQPRPLIRAPRCLRRNSSHLLPAEAVYRSPASYGLYGRYRRTSQVHNMGGLWAGKRSSLAARGSVALYRTSPPCWPDLSVSHTLQGTYYNPQIDTWSGFLSKAIAKSGLQHLSCQPSAAVLAKGETDREIRNTVDWSESAVYGEHIWFETNVSGDFCYVGEQHCYAKSLQKSVARKKCAACKIVVHSICIEQLEKLLDWNAPPQGEGLDEMPLKEPSFRINFRCKPSFRESGSRNIREPAIVRHHWVHRRRQEGKCKQCGKGFQQKFAFHSKEIVAISCSWCKQAYHNKVTCFMLQQIEEPCSLGAHAAVIVPPTWIIRVRRPQSSLKSSKKKKRTSFKRKSSKKGAEEARWKPFIVRPIPSQLMKPLLVFVNPKSGGNQGAKIIQTFLWYLNPRQVFDLSQGGPQEGLEMYRKVHNLRILACGGDGTVGWILSALDQLQLNPSPAVAVLPLGTGNDLARTLNWGGGYTDEPLSKILSHVEDGNIVQLDRWNLVVKPNPEAGPEERDEQVTDKLPLDVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGTAFSDFLMGSSKDLAKHIKVVCDGTDLTSKVQDLKLQCLVFLNIPRYCAGTMPWGNPSEHHDFEPQRHDDGCIEVIGFTMTSLATLQVGGHGERLNQCREVTLTTFKSIPMQVDGEPCKLAPSVIHISLRNQANMVQKTKRRTSIPLLNDSQQPFPERLRIRVSRISMHDYEALHYDKEKLKEASIPLGLIVVPGDSDLETCRAHIERLQEDFVSCNPSLQRLVLQEGDGSKSKALSSQRLSPKWCFLDSTTADRFYRIDRAQEHLNYVTEISQDELFVLDPELVVTETVSTSPGMPDLVDSGEVQSDPHKFAFPSSSSSPPNTPGPRVAELQRKRVSSDSSVAEALAHSDSLRSAKPALCRFEKSAVNNANSVSVDSLIECVKKKDHQKLKELHKKGADLCVQDPAGRTLLHYAVEVGSKEIVKYIIDNAPTDILDVTEKENGETVLHKAASLCHRTICHYLVEAGASLMKTDLQGDTPKHRAEKAKDAELAAYLENRQHYQMIQREDQETAV

Target ORF information:

RefSeq Version	XM_005169032.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005169032.3

ORF Insert Sequence:

ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT 
TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG 
CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA 
GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT 
CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC 
CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC 
CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA 
TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA 
CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC 
CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT 
GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG 
ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC 
AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC 
CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA 
ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG 
ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG 
CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC 
ATTGAACAGC TGGAGAAGTT ATTGGATTGG AATGCTCCAC CTCAGGGGGA GGGGCTTGAT 
GAGATGCCTC TCAAAGAGCC GTCATTCAGA ATAAACTTCA GATGTAAACC GTCTTTCAGG 
GAGTCAGGAT CACGGAACAT CCGAGAGCCT GCAATAGTGC GACACCACTG GGTCCATCGG 
AGAAGACAAG AGGGCAAATG CAAACAGTGT GGAAAGGGGT TTCAGCAGAA ATTTGCCTTT 
CACAGTAAAG AGATTGTAGC CATCAGCTGC TCCTGGTGCA AACAAGCATA TCATAATAAG 
GTGACATGCT TTATGCTGCA GCAGATAGAA GAACCATGTT CATTAGGAGC TCATGCCGCT 
GTAATAGTAC CTCCTACTTG GATCATACGG GTTCGCCGAC CTCAGTCCTC ACTAAAGTCA 
AGCAAAAAGA AAAAACGGAC ATCTTTCAAA CGTAAATCCA GCAAGAAAGG AGCAGAGGAG 
GCACGATGGA AGCCTTTTAT AGTGAGACCC ATTCCCTCCC AACTCATGAA GCCACTGCTA 
GTGTTTGTAA ATCCAAAGAG TGGAGGAAAC CAGGGAGCTA AAATCATCCA GACCTTTCTT 
TGGTATCTAA ACCCTCGGCA GGTGTTTGAT CTAAGTCAAG GAGGTCCTCA GGAAGGCTTG 
GAAATGTATC GCAAGGTTCA CAACTTGAGA ATTTTGGCCT GTGGGGGGGA TGGCACGGTG 
GGCTGGATCT TATCTGCACT TGATCAGCTG CAATTAAACC CTTCTCCTGC TGTGGCCGTA 
CTTCCCCTGG GCACAGGAAA TGACCTTGCT AGGACGCTAA ACTGGGGTGG GGGCTACACA 
GATGAACCTC TGAGTAAAAT CCTGTCTCAT GTGGAGGATG GGAATATTGT ACAGCTGGAT 
CGGTGGAATC TTGTTGTGAA GCCCAACCCA GAGGCAGGAC CAGAGGAAAG AGACGAGCAG 
GTGACGGACA AGCTCCCTCT AGACGTCTTC AATAACTACT TCAGCCTTGG ATTTGATGCT 
CATGTGACTT TAGAGTTCCA CGAATCTCGA GAGGCTAATC CTGAGAAATT TAACAGCCGG 
TTCAGAAACA AAATGTTCTA TGCAGGGACA GCGTTTTCTG ATTTCTTGAT GGGCAGCTCC 
AAAGACCTGG CTAAGCACAT CAAAGTAGTC TGTGACGGCA CAGATTTAAC CTCAAAGGTC 
CAGGATTTGA AGCTGCAATG CCTGGTCTTC CTAAATATCC CCAGGTACTG TGCGGGCACA 
ATGCCATGGG GCAATCCGAG TGAGCATCAT GACTTTGAGC CTCAGCGTCA TGATGATGGC 
TGCATTGAGG TTATTGGATT CACAATGACA TCACTGGCCA CACTGCAGGT CGGAGGTCAC 
GGTGAGAGGT TAAATCAGTG TCGCGAAGTC ACCCTCACCA CCTTTAAGTC AATCCCCATG 
CAGGTGGACG GGGAGCCTTG TAAACTAGCA CCATCTGTTA TTCACATTTC TTTGCGAAAC 
CAGGCCAACA TGGTGCAGAA AACCAAGAGA CGTACATCTA TACCACTGCT AAACGACAGT 
CAGCAACCGT TTCCAGAACG ACTACGGATC AGAGTGAGCC GTATCAGCAT GCATGATTAT 
GAAGCTTTGC ACTACGACAA GGAGAAGCTC AAAGAAGCTT CGATTCCACT GGGTCTCATC 
GTTGTCCCTG GAGACAGTGA TTTGGAAACC TGCCGAGCTC ACATCGAGAG ACTACAGGAG 
GACTTTGTGT CGTGTAACCC GTCTCTGCAG CGGCTGGTGC TCCAGGAAGG TGACGGGAGC 
AAATCAAAGG CTTTGTCGTC TCAGAGGCTA TCACCGAAAT GGTGCTTTCT CGACTCTACA 
ACTGCTGATC GATTCTACAG GATTGATCGA GCTCAAGAGC ATCTCAACTA CGTGACTGAA 
ATTTCTCAGG ATGAGCTGTT TGTTTTGGAC CCAGAGCTGG TGGTCACAGA GACTGTTAGC 
ACATCACCTG GCATGCCAGA CCTGGTGGAC TCTGGTGAAG TCCAGTCCGA TCCACACAAA 
TTTGCCTTTC CGTCATCCTC TTCTTCACCA CCAAACACAC CTGGACCTAG GGTTGCAGAG 
CTGCAAAGGA AGCGTGTTTC CAGTGACAGT TCGGTGGCAG AAGCACTTGC ACACAGTGAC 
TCGCTCAGAT CAGCCAAGCC AGCTCTCTGC AGGTTTGAGA AGAGCGCTGT GAACAATGCC 
AACTCTGTGT CAGTGGACAG TCTAATTGAA TGTGTGAAAA AGAAGGACCA TCAGAAGTTG 
AAGGAGCTGC ATAAGAAAGG GGCCGACCTG TGTGTGCAGG ACCCTGCTGG ACGGACCCTA 
TTGCACTATG CTGTGGAAGT GGGCAGCAAG GAGATTGTCA AATACATCAT TGATAATGCA 
CCCACCGATA TCCTGGATGT AACAGAGAAA GAAAATGGAG AGACTGTGTT GCACAAAGCA 
GCCTCGCTGT GTCATAGGAC AATCTGTCAT TACCTAGTGG AAGCAGGAGC ATCTCTTATG 
AAGACAGACT TACAGGGTGA CACTCCGAAG CATCGTGCCG AGAAGGCGAA AGATGCTGAA 
CTTGCGGCAT ATCTTGAAAA CCGCCAGCAC TACCAAATGA TCCAGCGTGA GGACCAGGAG 
ACTGCAGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dgkza ( XM_005169033.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005169090.1 Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa13281
Clone ID Related Accession (Same CDS sequence)	XM_005169033.3 , XM_005169033.4
Accession Version	XM_005169033.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	diacylglycerol kinase zeta isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169033.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT 
TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG 
CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA 
GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT 
CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC 
CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC 
CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA 
TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA 
CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC 
CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT 
GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG 
ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC 
AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC 
CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA 
ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG 
ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG 
CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC 
ATTGAACAGC TGGAGAAGAT AAACTTCAGA TGTAAACCGT CTTTCAGGGA GTCAGGATCA 
CGGAACATCC GAGAGCCTGC AATAGTGCGA CACCACTGGG TCCATCGGAG AAGACAAGAG 
GGCAAATGCA AACAGTGTGG AAAGGGGTTT CAGCAGAAAT TTGCCTTTCA CAGTAAAGAG 
ATTGTAGCCA TCAGCTGCTC CTGGTGCAAA CAAGCATATC ATAATAAGGT GACATGCTTT 
ATGCTGCAGC AGATAGAAGA ACCATGTTCA TTAGGAGCTC ATGCCGCTGT AATAGTACCT 
CCTACTTGGA TCATACGGGT TCGCCGACCT CAGTCCTCAC TAAAGTCAAG CAAAAAGAAA 
AAACGGACAT CTTTCAAACG TAAATCCAGC AAGAAAGGAG CAGAGGAGGC ACGATGGAAG 
CCTTTTATAG TGAGACCCAT TCCCTCCCAA CTCATGAAGC CACTGCTAGT GTTTGTAAAT 
CCAAAGAGTG GAGGAAACCA GGGAGCTAAA ATCATCCAGA CCTTTCTTTG GTATCTAAAC 
CCTCGGCAGG TGTTTGATCT AAGTCAAGGA GGTCCTCAGG AAGGCTTGGA AATGTATCGC 
AAGGTTCACA ACTTGAGAAT TTTGGCCTGT GGGGGGGATG GCACGGTGGG CTGGATCTTA 
TCTGCACTTG ATCAGCTGCA ATTAAACCCT TCTCCTGCTG TGGCCGTACT TCCCCTGGGC 
ACAGGAAATG ACCTTGCTAG GACGCTAAAC TGGGGTGGGG GCTACACAGA TGAACCTCTG 
AGTAAAATCC TGTCTCATGT GGAGGATGGG AATATTGTAC AGCTGGATCG GTGGAATCTT 
GTTGTGAAGC CCAACCCAGA GGCAGGACCA GAGGAAAGAG ACGAGCAGGT GACGGACAAG 
CTCCCTCTAG ACGTCTTCAA TAACTACTTC AGCCTTGGAT TTGATGCTCA TGTGACTTTA 
GAGTTCCACG AATCTCGAGA GGCTAATCCT GAGAAATTTA ACAGCCGGTT CAGAAACAAA 
ATGTTCTATG CAGGGACAGC GTTTTCTGAT TTCTTGATGG GCAGCTCCAA AGACCTGGCT 
AAGCACATCA AAGTAGTCTG TGACGGCACA GATTTAACCT CAAAGGTCCA GGATTTGAAG 
CTGCAATGCC TGGTCTTCCT AAATATCCCC AGGTACTGTG CGGGCACAAT GCCATGGGGC 
AATCCGAGTG AGCATCATGA CTTTGAGCCT CAGCGTCATG ATGATGGCTG CATTGAGGTT 
ATTGGATTCA CAATGACATC ACTGGCCACA CTGCAGGTCG GAGGTCACGG TGAGAGGTTA 
AATCAGTGTC GCGAAGTCAC CCTCACCACC TTTAAGTCAA TCCCCATGCA GGTGGACGGG 
GAGCCTTGTA AACTAGCACC ATCTGTTATT CACATTTCTT TGCGAAACCA GGCCAACATG 
GTGCAGAAAA CCAAGAGACG TACATCTATA CCACTGCTAA ACGACAGTCA GCAACCGTTT 
CCAGAACGAC TACGGATCAG AGTGAGCCGT ATCAGCATGC ATGATTATGA AGCTTTGCAC 
TACGACAAGG AGAAGCTCAA AGAAGCTTCG ATTCCACTGG GTCTCATCGT TGTCCCTGGA 
GACAGTGATT TGGAAACCTG CCGAGCTCAC ATCGAGAGAC TACAGGAGGA CTTTGTGTCG 
TGTAACCCGT CTCTGCAGCG GCTGGTGCTC CAGGAAGGTG ACGGGAGCAA ATCAAAGGCT 
TTGTCGTCTC AGAGGCTATC ACCGAAATGG TGCTTTCTCG ACTCTACAAC TGCTGATCGA 
TTCTACAGGA TTGATCGAGC TCAAGAGCAT CTCAACTACG TGACTGAAAT TTCTCAGGAT 
GAGCTGTTTG TTTTGGACCC AGAGCTGGTG GTCACAGAGA CTGTTAGCAC ATCACCTGGC 
ATGCCAGACC TGGTGGACTC TGGTGAAGTC CAGTCCGATC CACACAAATT TGCCTTTCCG 
TCATCCTCTT CTTCACCACC AAACACACCT GGACCTAGGG TTGCAGAGCT GCAAAGGAAG 
CGTGTTTCCA GTGACAGTTC GGTGGCAGAA GCACTTGCAC ACAGTGACTC GCTCAGATCA 
GCCAAGCCAG CTCTCTGCAG AGCTGGGGGT GTTCATCGCT CAAATACAAC CGCAGCAGAT 
TTCAAACCTA CCAGCAGGTT TGAGAAGAGC GCTGTGAACA ATGCCAACTC TGTGTCAGTG 
GACAGTCTAA TTGAATGTGT GAAAAAGAAG GACCATCAGA AGTTGAAGGA GCTGCATAAG 
AAAGGGGCCG ACCTGTGTGT GCAGGACCCT GCTGGACGGA CCCTATTGCA CTATGCTGTG 
GAAGTGGGCA GCAAGGAGAT TGTCAAATAC ATCATTGATA ATGCACCCAC CGATATCCTG 
GATGTAACAG AGAAAGAAAA TGGAGAGACT GTGTTGCACA AAGCAGCCTC GCTGTGTCAT 
AGGACAATCT GTCATTACCT AGTGGAAGCA GGAGCATCTC TTATGAAGAC AGACTTACAG 
GGTGACACTC CGAAGCATCG TGCCGAGAAG GCGAAAGATG CTGAACTTGC GGCATATCTT 
GAAAACCGCC AGCACTACCA AATGATCCAG CGTGAGGACC AGGAGACTGC AGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005169090.1
CDS	136..3852
Translation	MDALFNLRFRRKAVEPSGRRRSSVSLPASKARRRSSAGLPSAGLAHRRRSSAQLQIGSIQPGLVLHRRGHFTRRKSSGGTACLTPRFAIRRRQAAKHRSIHAQLLGPSLLLASVVQMSEDHEPEDKSAEEYSSLSDTDCSQDEHEQTGMDFETESDKCLTWLAQGLLVGESIQPRPLIRAPRCLRRNSSHLLPAEAVYRSPASYGLYGRYRRTSQVHNMGGLWAGKRSSLAARGSVALYRTSPPCWPDLSVSHTLQGTYYNPQIDTWSGFLSKAIAKSGLQHLSCQPSAAVLAKGETDREIRNTVDWSESAVYGEHIWFETNVSGDFCYVGEQHCYAKSLQKSVARKKCAACKIVVHSICIEQLEKINFRCKPSFRESGSRNIREPAIVRHHWVHRRRQEGKCKQCGKGFQQKFAFHSKEIVAISCSWCKQAYHNKVTCFMLQQIEEPCSLGAHAAVIVPPTWIIRVRRPQSSLKSSKKKKRTSFKRKSSKKGAEEARWKPFIVRPIPSQLMKPLLVFVNPKSGGNQGAKIIQTFLWYLNPRQVFDLSQGGPQEGLEMYRKVHNLRILACGGDGTVGWILSALDQLQLNPSPAVAVLPLGTGNDLARTLNWGGGYTDEPLSKILSHVEDGNIVQLDRWNLVVKPNPEAGPEERDEQVTDKLPLDVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGTAFSDFLMGSSKDLAKHIKVVCDGTDLTSKVQDLKLQCLVFLNIPRYCAGTMPWGNPSEHHDFEPQRHDDGCIEVIGFTMTSLATLQVGGHGERLNQCREVTLTTFKSIPMQVDGEPCKLAPSVIHISLRNQANMVQKTKRRTSIPLLNDSQQPFPERLRIRVSRISMHDYEALHYDKEKLKEASIPLGLIVVPGDSDLETCRAHIERLQEDFVSCNPSLQRLVLQEGDGSKSKALSSQRLSPKWCFLDSTTADRFYRIDRAQEHLNYVTEISQDELFVLDPELVVTETVSTSPGMPDLVDSGEVQSDPHKFAFPSSSSSPPNTPGPRVAELQRKRVSSDSSVAEALAHSDSLRSAKPALCRAGGVHRSNTTAADFKPTSRFEKSAVNNANSVSVDSLIECVKKKDHQKLKELHKKGADLCVQDPAGRTLLHYAVEVGSKEIVKYIIDNAPTDILDVTEKENGETVLHKAASLCHRTICHYLVEAGASLMKTDLQGDTPKHRAEKAKDAELAAYLENRQHYQMIQREDQETAV

Target ORF information:

RefSeq Version	XM_005169033.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005169033.3

ORF Insert Sequence:

ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT 
TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG 
CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA 
GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT 
CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC 
CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC 
CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA 
TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA 
CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC 
CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT 
GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG 
ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC 
AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC 
CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA 
ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG 
ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG 
CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC 
ATTGAACAGC TGGAGAAGAT AAACTTCAGA TGTAAACCGT CTTTCAGGGA GTCAGGATCA 
CGGAACATCC GAGAGCCTGC AATAGTGCGA CACCACTGGG TCCATCGGAG AAGACAAGAG 
GGCAAATGCA AACAGTGTGG AAAGGGGTTT CAGCAGAAAT TTGCCTTTCA CAGTAAAGAG 
ATTGTAGCCA TCAGCTGCTC CTGGTGCAAA CAAGCATATC ATAATAAGGT GACATGCTTT 
ATGCTGCAGC AGATAGAAGA ACCATGTTCA TTAGGAGCTC ATGCCGCTGT AATAGTACCT 
CCTACTTGGA TCATACGGGT TCGCCGACCT CAGTCCTCAC TAAAGTCAAG CAAAAAGAAA 
AAACGGACAT CTTTCAAACG TAAATCCAGC AAGAAAGGAG CAGAGGAGGC ACGATGGAAG 
CCTTTTATAG TGAGACCCAT TCCCTCCCAA CTCATGAAGC CACTGCTAGT GTTTGTAAAT 
CCAAAGAGTG GAGGAAACCA GGGAGCTAAA ATCATCCAGA CCTTTCTTTG GTATCTAAAC 
CCTCGGCAGG TGTTTGATCT AAGTCAAGGA GGTCCTCAGG AAGGCTTGGA AATGTATCGC 
AAGGTTCACA ACTTGAGAAT TTTGGCCTGT GGGGGGGATG GCACGGTGGG CTGGATCTTA 
TCTGCACTTG ATCAGCTGCA ATTAAACCCT TCTCCTGCTG TGGCCGTACT TCCCCTGGGC 
ACAGGAAATG ACCTTGCTAG GACGCTAAAC TGGGGTGGGG GCTACACAGA TGAACCTCTG 
AGTAAAATCC TGTCTCATGT GGAGGATGGG AATATTGTAC AGCTGGATCG GTGGAATCTT 
GTTGTGAAGC CCAACCCAGA GGCAGGACCA GAGGAAAGAG ACGAGCAGGT GACGGACAAG 
CTCCCTCTAG ACGTCTTCAA TAACTACTTC AGCCTTGGAT TTGATGCTCA TGTGACTTTA 
GAGTTCCACG AATCTCGAGA GGCTAATCCT GAGAAATTTA ACAGCCGGTT CAGAAACAAA 
ATGTTCTATG CAGGGACAGC GTTTTCTGAT TTCTTGATGG GCAGCTCCAA AGACCTGGCT 
AAGCACATCA AAGTAGTCTG TGACGGCACA GATTTAACCT CAAAGGTCCA GGATTTGAAG 
CTGCAATGCC TGGTCTTCCT AAATATCCCC AGGTACTGTG CGGGCACAAT GCCATGGGGC 
AATCCGAGTG AGCATCATGA CTTTGAGCCT CAGCGTCATG ATGATGGCTG CATTGAGGTT 
ATTGGATTCA CAATGACATC ACTGGCCACA CTGCAGGTCG GAGGTCACGG TGAGAGGTTA 
AATCAGTGTC GCGAAGTCAC CCTCACCACC TTTAAGTCAA TCCCCATGCA GGTGGACGGG 
GAGCCTTGTA AACTAGCACC ATCTGTTATT CACATTTCTT TGCGAAACCA GGCCAACATG 
GTGCAGAAAA CCAAGAGACG TACATCTATA CCACTGCTAA ACGACAGTCA GCAACCGTTT 
CCAGAACGAC TACGGATCAG AGTGAGCCGT ATCAGCATGC ATGATTATGA AGCTTTGCAC 
TACGACAAGG AGAAGCTCAA AGAAGCTTCG ATTCCACTGG GTCTCATCGT TGTCCCTGGA 
GACAGTGATT TGGAAACCTG CCGAGCTCAC ATCGAGAGAC TACAGGAGGA CTTTGTGTCG 
TGTAACCCGT CTCTGCAGCG GCTGGTGCTC CAGGAAGGTG ACGGGAGCAA ATCAAAGGCT 
TTGTCGTCTC AGAGGCTATC ACCGAAATGG TGCTTTCTCG ACTCTACAAC TGCTGATCGA 
TTCTACAGGA TTGATCGAGC TCAAGAGCAT CTCAACTACG TGACTGAAAT TTCTCAGGAT 
GAGCTGTTTG TTTTGGACCC AGAGCTGGTG GTCACAGAGA CTGTTAGCAC ATCACCTGGC 
ATGCCAGACC TGGTGGACTC TGGTGAAGTC CAGTCCGATC CACACAAATT TGCCTTTCCG 
TCATCCTCTT CTTCACCACC AAACACACCT GGACCTAGGG TTGCAGAGCT GCAAAGGAAG 
CGTGTTTCCA GTGACAGTTC GGTGGCAGAA GCACTTGCAC ACAGTGACTC GCTCAGATCA 
GCCAAGCCAG CTCTCTGCAG AGCTGGGGGT GTTCATCGCT CAAATACAAC CGCAGCAGAT 
TTCAAACCTA CCAGCAGGTT TGAGAAGAGC GCTGTGAACA ATGCCAACTC TGTGTCAGTG 
GACAGTCTAA TTGAATGTGT GAAAAAGAAG GACCATCAGA AGTTGAAGGA GCTGCATAAG 
AAAGGGGCCG ACCTGTGTGT GCAGGACCCT GCTGGACGGA CCCTATTGCA CTATGCTGTG 
GAAGTGGGCA GCAAGGAGAT TGTCAAATAC ATCATTGATA ATGCACCCAC CGATATCCTG 
GATGTAACAG AGAAAGAAAA TGGAGAGACT GTGTTGCACA AAGCAGCCTC GCTGTGTCAT 
AGGACAATCT GTCATTACCT AGTGGAAGCA GGAGCATCTC TTATGAAGAC AGACTTACAG 
GGTGACACTC CGAAGCATCG TGCCGAGAAG GCGAAAGATG CTGAACTTGC GGCATATCTT 
GAAAACCGCC AGCACTACCA AATGATCCAG CGTGAGGACC AGGAGACTGC AGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dgkza ( XM_005169033.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005169090.1 Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X3, mRNA.

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CloneID	ODa13281
Clone ID Related Accession (Same CDS sequence)	XM_005169033.3 , XM_005169033.4
Accession Version	XM_005169033.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	diacylglycerol kinase zeta isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005169033.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT 
TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG 
CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA 
GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT 
CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC 
CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC 
CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA 
TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA 
CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC 
CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT 
GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG 
ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC 
AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC 
CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA 
ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG 
ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG 
CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC 
ATTGAACAGC TGGAGAAGAT AAACTTCAGA TGTAAACCGT CTTTCAGGGA GTCAGGATCA 
CGGAACATCC GAGAGCCTGC AATAGTGCGA CACCACTGGG TCCATCGGAG AAGACAAGAG 
GGCAAATGCA AACAGTGTGG AAAGGGGTTT CAGCAGAAAT TTGCCTTTCA CAGTAAAGAG 
ATTGTAGCCA TCAGCTGCTC CTGGTGCAAA CAAGCATATC ATAATAAGGT GACATGCTTT 
ATGCTGCAGC AGATAGAAGA ACCATGTTCA TTAGGAGCTC ATGCCGCTGT AATAGTACCT 
CCTACTTGGA TCATACGGGT TCGCCGACCT CAGTCCTCAC TAAAGTCAAG CAAAAAGAAA 
AAACGGACAT CTTTCAAACG TAAATCCAGC AAGAAAGGAG CAGAGGAGGC ACGATGGAAG 
CCTTTTATAG TGAGACCCAT TCCCTCCCAA CTCATGAAGC CACTGCTAGT GTTTGTAAAT 
CCAAAGAGTG GAGGAAACCA GGGAGCTAAA ATCATCCAGA CCTTTCTTTG GTATCTAAAC 
CCTCGGCAGG TGTTTGATCT AAGTCAAGGA GGTCCTCAGG AAGGCTTGGA AATGTATCGC 
AAGGTTCACA ACTTGAGAAT TTTGGCCTGT GGGGGGGATG GCACGGTGGG CTGGATCTTA 
TCTGCACTTG ATCAGCTGCA ATTAAACCCT TCTCCTGCTG TGGCCGTACT TCCCCTGGGC 
ACAGGAAATG ACCTTGCTAG GACGCTAAAC TGGGGTGGGG GCTACACAGA TGAACCTCTG 
AGTAAAATCC TGTCTCATGT GGAGGATGGG AATATTGTAC AGCTGGATCG GTGGAATCTT 
GTTGTGAAGC CCAACCCAGA GGCAGGACCA GAGGAAAGAG ACGAGCAGGT GACGGACAAG 
CTCCCTCTAG ACGTCTTCAA TAACTACTTC AGCCTTGGAT TTGATGCTCA TGTGACTTTA 
GAGTTCCACG AATCTCGAGA GGCTAATCCT GAGAAATTTA ACAGCCGGTT CAGAAACAAA 
ATGTTCTATG CAGGGACAGC GTTTTCTGAT TTCTTGATGG GCAGCTCCAA AGACCTGGCT 
AAGCACATCA AAGTAGTCTG TGACGGCACA GATTTAACCT CAAAGGTCCA GGATTTGAAG 
CTGCAATGCC TGGTCTTCCT AAATATCCCC AGGTACTGTG CGGGCACAAT GCCATGGGGC 
AATCCGAGTG AGCATCATGA CTTTGAGCCT CAGCGTCATG ATGATGGCTG CATTGAGGTT 
ATTGGATTCA CAATGACATC ACTGGCCACA CTGCAGGTCG GAGGTCACGG TGAGAGGTTA 
AATCAGTGTC GCGAAGTCAC CCTCACCACC TTTAAGTCAA TCCCCATGCA GGTGGACGGG 
GAGCCTTGTA AACTAGCACC ATCTGTTATT CACATTTCTT TGCGAAACCA GGCCAACATG 
GTGCAGAAAA CCAAGAGACG TACATCTATA CCACTGCTAA ACGACAGTCA GCAACCGTTT 
CCAGAACGAC TACGGATCAG AGTGAGCCGT ATCAGCATGC ATGATTATGA AGCTTTGCAC 
TACGACAAGG AGAAGCTCAA AGAAGCTTCG ATTCCACTGG GTCTCATCGT TGTCCCTGGA 
GACAGTGATT TGGAAACCTG CCGAGCTCAC ATCGAGAGAC TACAGGAGGA CTTTGTGTCG 
TGTAACCCGT CTCTGCAGCG GCTGGTGCTC CAGGAAGGTG ACGGGAGCAA ATCAAAGGCT 
TTGTCGTCTC AGAGGCTATC ACCGAAATGG TGCTTTCTCG ACTCTACAAC TGCTGATCGA 
TTCTACAGGA TTGATCGAGC TCAAGAGCAT CTCAACTACG TGACTGAAAT TTCTCAGGAT 
GAGCTGTTTG TTTTGGACCC AGAGCTGGTG GTCACAGAGA CTGTTAGCAC ATCACCTGGC 
ATGCCAGACC TGGTGGACTC TGGTGAAGTC CAGTCCGATC CACACAAATT TGCCTTTCCG 
TCATCCTCTT CTTCACCACC AAACACACCT GGACCTAGGG TTGCAGAGCT GCAAAGGAAG 
CGTGTTTCCA GTGACAGTTC GGTGGCAGAA GCACTTGCAC ACAGTGACTC GCTCAGATCA 
GCCAAGCCAG CTCTCTGCAG AGCTGGGGGT GTTCATCGCT CAAATACAAC CGCAGCAGAT 
TTCAAACCTA CCAGCAGGTT TGAGAAGAGC GCTGTGAACA ATGCCAACTC TGTGTCAGTG 
GACAGTCTAA TTGAATGTGT GAAAAAGAAG GACCATCAGA AGTTGAAGGA GCTGCATAAG 
AAAGGGGCCG ACCTGTGTGT GCAGGACCCT GCTGGACGGA CCCTATTGCA CTATGCTGTG 
GAAGTGGGCA GCAAGGAGAT TGTCAAATAC ATCATTGATA ATGCACCCAC CGATATCCTG 
GATGTAACAG AGAAAGAAAA TGGAGAGACT GTGTTGCACA AAGCAGCCTC GCTGTGTCAT 
AGGACAATCT GTCATTACCT AGTGGAAGCA GGAGCATCTC TTATGAAGAC AGACTTACAG 
GGTGACACTC CGAAGCATCG TGCCGAGAAG GCGAAAGATG CTGAACTTGC GGCATATCTT 
GAAAACCGCC AGCACTACCA AATGATCCAG CGTGAGGACC AGGAGACTGC AGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005169090.1
CDS	171..3887
Translation	MDALFNLRFRRKAVEPSGRRRSSVSLPASKARRRSSAGLPSAGLAHRRRSSAQLQIGSIQPGLVLHRRGHFTRRKSSGGTACLTPRFAIRRRQAAKHRSIHAQLLGPSLLLASVVQMSEDHEPEDKSAEEYSSLSDTDCSQDEHEQTGMDFETESDKCLTWLAQGLLVGESIQPRPLIRAPRCLRRNSSHLLPAEAVYRSPASYGLYGRYRRTSQVHNMGGLWAGKRSSLAARGSVALYRTSPPCWPDLSVSHTLQGTYYNPQIDTWSGFLSKAIAKSGLQHLSCQPSAAVLAKGETDREIRNTVDWSESAVYGEHIWFETNVSGDFCYVGEQHCYAKSLQKSVARKKCAACKIVVHSICIEQLEKINFRCKPSFRESGSRNIREPAIVRHHWVHRRRQEGKCKQCGKGFQQKFAFHSKEIVAISCSWCKQAYHNKVTCFMLQQIEEPCSLGAHAAVIVPPTWIIRVRRPQSSLKSSKKKKRTSFKRKSSKKGAEEARWKPFIVRPIPSQLMKPLLVFVNPKSGGNQGAKIIQTFLWYLNPRQVFDLSQGGPQEGLEMYRKVHNLRILACGGDGTVGWILSALDQLQLNPSPAVAVLPLGTGNDLARTLNWGGGYTDEPLSKILSHVEDGNIVQLDRWNLVVKPNPEAGPEERDEQVTDKLPLDVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGTAFSDFLMGSSKDLAKHIKVVCDGTDLTSKVQDLKLQCLVFLNIPRYCAGTMPWGNPSEHHDFEPQRHDDGCIEVIGFTMTSLATLQVGGHGERLNQCREVTLTTFKSIPMQVDGEPCKLAPSVIHISLRNQANMVQKTKRRTSIPLLNDSQQPFPERLRIRVSRISMHDYEALHYDKEKLKEASIPLGLIVVPGDSDLETCRAHIERLQEDFVSCNPSLQRLVLQEGDGSKSKALSSQRLSPKWCFLDSTTADRFYRIDRAQEHLNYVTEISQDELFVLDPELVVTETVSTSPGMPDLVDSGEVQSDPHKFAFPSSSSSPPNTPGPRVAELQRKRVSSDSSVAEALAHSDSLRSAKPALCRAGGVHRSNTTAADFKPTSRFEKSAVNNANSVSVDSLIECVKKKDHQKLKELHKKGADLCVQDPAGRTLLHYAVEVGSKEIVKYIIDNAPTDILDVTEKENGETVLHKAASLCHRTICHYLVEAGASLMKTDLQGDTPKHRAEKAKDAELAAYLENRQHYQMIQREDQETAV

Target ORF information:

RefSeq Version	XM_005169033.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005169033.4

ORF Insert Sequence:

ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT 
TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG 
CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA 
GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT 
CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC 
CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC 
CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA 
TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA 
CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC 
CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT 
GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG 
ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC 
AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC 
CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA 
ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG 
ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG 
CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC 
ATTGAACAGC TGGAGAAGAT AAACTTCAGA TGTAAACCGT CTTTCAGGGA GTCAGGATCA 
CGGAACATCC GAGAGCCTGC AATAGTGCGA CACCACTGGG TCCATCGGAG AAGACAAGAG 
GGCAAATGCA AACAGTGTGG AAAGGGGTTT CAGCAGAAAT TTGCCTTTCA CAGTAAAGAG 
ATTGTAGCCA TCAGCTGCTC CTGGTGCAAA CAAGCATATC ATAATAAGGT GACATGCTTT 
ATGCTGCAGC AGATAGAAGA ACCATGTTCA TTAGGAGCTC ATGCCGCTGT AATAGTACCT 
CCTACTTGGA TCATACGGGT TCGCCGACCT CAGTCCTCAC TAAAGTCAAG CAAAAAGAAA 
AAACGGACAT CTTTCAAACG TAAATCCAGC AAGAAAGGAG CAGAGGAGGC ACGATGGAAG 
CCTTTTATAG TGAGACCCAT TCCCTCCCAA CTCATGAAGC CACTGCTAGT GTTTGTAAAT 
CCAAAGAGTG GAGGAAACCA GGGAGCTAAA ATCATCCAGA CCTTTCTTTG GTATCTAAAC 
CCTCGGCAGG TGTTTGATCT AAGTCAAGGA GGTCCTCAGG AAGGCTTGGA AATGTATCGC 
AAGGTTCACA ACTTGAGAAT TTTGGCCTGT GGGGGGGATG GCACGGTGGG CTGGATCTTA 
TCTGCACTTG ATCAGCTGCA ATTAAACCCT TCTCCTGCTG TGGCCGTACT TCCCCTGGGC 
ACAGGAAATG ACCTTGCTAG GACGCTAAAC TGGGGTGGGG GCTACACAGA TGAACCTCTG 
AGTAAAATCC TGTCTCATGT GGAGGATGGG AATATTGTAC AGCTGGATCG GTGGAATCTT 
GTTGTGAAGC CCAACCCAGA GGCAGGACCA GAGGAAAGAG ACGAGCAGGT GACGGACAAG 
CTCCCTCTAG ACGTCTTCAA TAACTACTTC AGCCTTGGAT TTGATGCTCA TGTGACTTTA 
GAGTTCCACG AATCTCGAGA GGCTAATCCT GAGAAATTTA ACAGCCGGTT CAGAAACAAA 
ATGTTCTATG CAGGGACAGC GTTTTCTGAT TTCTTGATGG GCAGCTCCAA AGACCTGGCT 
AAGCACATCA AAGTAGTCTG TGACGGCACA GATTTAACCT CAAAGGTCCA GGATTTGAAG 
CTGCAATGCC TGGTCTTCCT AAATATCCCC AGGTACTGTG CGGGCACAAT GCCATGGGGC 
AATCCGAGTG AGCATCATGA CTTTGAGCCT CAGCGTCATG ATGATGGCTG CATTGAGGTT 
ATTGGATTCA CAATGACATC ACTGGCCACA CTGCAGGTCG GAGGTCACGG TGAGAGGTTA 
AATCAGTGTC GCGAAGTCAC CCTCACCACC TTTAAGTCAA TCCCCATGCA GGTGGACGGG 
GAGCCTTGTA AACTAGCACC ATCTGTTATT CACATTTCTT TGCGAAACCA GGCCAACATG 
GTGCAGAAAA CCAAGAGACG TACATCTATA CCACTGCTAA ACGACAGTCA GCAACCGTTT 
CCAGAACGAC TACGGATCAG AGTGAGCCGT ATCAGCATGC ATGATTATGA AGCTTTGCAC 
TACGACAAGG AGAAGCTCAA AGAAGCTTCG ATTCCACTGG GTCTCATCGT TGTCCCTGGA 
GACAGTGATT TGGAAACCTG CCGAGCTCAC ATCGAGAGAC TACAGGAGGA CTTTGTGTCG 
TGTAACCCGT CTCTGCAGCG GCTGGTGCTC CAGGAAGGTG ACGGGAGCAA ATCAAAGGCT 
TTGTCGTCTC AGAGGCTATC ACCGAAATGG TGCTTTCTCG ACTCTACAAC TGCTGATCGA 
TTCTACAGGA TTGATCGAGC TCAAGAGCAT CTCAACTACG TGACTGAAAT TTCTCAGGAT 
GAGCTGTTTG TTTTGGACCC AGAGCTGGTG GTCACAGAGA CTGTTAGCAC ATCACCTGGC 
ATGCCAGACC TGGTGGACTC TGGTGAAGTC CAGTCCGATC CACACAAATT TGCCTTTCCG 
TCATCCTCTT CTTCACCACC AAACACACCT GGACCTAGGG TTGCAGAGCT GCAAAGGAAG 
CGTGTTTCCA GTGACAGTTC GGTGGCAGAA GCACTTGCAC ACAGTGACTC GCTCAGATCA 
GCCAAGCCAG CTCTCTGCAG AGCTGGGGGT GTTCATCGCT CAAATACAAC CGCAGCAGAT 
TTCAAACCTA CCAGCAGGTT TGAGAAGAGC GCTGTGAACA ATGCCAACTC TGTGTCAGTG 
GACAGTCTAA TTGAATGTGT GAAAAAGAAG GACCATCAGA AGTTGAAGGA GCTGCATAAG 
AAAGGGGCCG ACCTGTGTGT GCAGGACCCT GCTGGACGGA CCCTATTGCA CTATGCTGTG 
GAAGTGGGCA GCAAGGAGAT TGTCAAATAC ATCATTGATA ATGCACCCAC CGATATCCTG 
GATGTAACAG AGAAAGAAAA TGGAGAGACT GTGTTGCACA AAGCAGCCTC GCTGTGTCAT 
AGGACAATCT GTCATTACCT AGTGGAAGCA GGAGCATCTC TTATGAAGAC AGACTTACAG 
GGTGACACTC CGAAGCATCG TGCCGAGAAG GCGAAAGATG CTGAACTTGC GGCATATCTT 
GAAAACCGCC AGCACTACCA AATGATCCAG CGTGAGGACC AGGAGACTGC AGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dgkza ( XM_005169034.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005169091.1 Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X8, mRNA.

Price & Availability↑

CloneID	ODa13282
Clone ID Related Accession (Same CDS sequence)	XM_005169034.3 , XM_005169034.4
Accession Version	XM_005169034.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	diacylglycerol kinase zeta isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169034.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT 
TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG 
CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA 
GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT 
CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC 
CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC 
CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA 
TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA 
CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC 
CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT 
GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG 
ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC 
AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC 
CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA 
ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG 
ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG 
CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC 
ATTGAACAGC TGGAGAAGAT AAACTTCAGA TGTAAACCGT CTTTCAGGGA GTCAGGATCA 
CGGAACATCC GAGAGCCTGC AATAGTGCGA CACCACTGGG TCCATCGGAG AAGACAAGAG 
GGCAAATGCA AACAGTGTGG AAAGGGGTTT CAGCAGAAAT TTGCCTTTCA CAGTAAAGAG 
ATTGTAGCCA TCAGCTGCTC CTGGTGCAAA CAAGCATATC ATAATAAGGT GACATGCTTT 
ATGCTGCAGC AGATAGAAGA ACCATGTTCA TTAGGAGCTC ATGCCGCTGT AATAGTACCT 
CCTACTTGGA TCATACGGGT TCGCCGACCT CAGTCCTCAC TAAAGTCAAG CAAAAAGAAA 
AAACGGACAT CTTTCAAACG TAAATCCAGC AAGAAAGGAG CAGAGGAGGC ACGATGGAAG 
CCTTTTATAG TGAGACCCAT TCCCTCCCAA CTCATGAAGC CACTGCTAGT GTTTGTAAAT 
CCAAAGAGTG GAGGAAACCA GGGAGCTAAA ATCATCCAGA CCTTTCTTTG GTATCTAAAC 
CCTCGGCAGG TGTTTGATCT AAGTCAAGGA GGTCCTCAGG AAGGCTTGGA AATGTATCGC 
AAGGTTCACA ACTTGAGAAT TTTGGCCTGT GGGGGGGATG GCACGGTGGG CTGGATCTTA 
TCTGCACTTG ATCAGCTGCA ATTAAACCCT TCTCCTGCTG TGGCCGTACT TCCCCTGGGC 
ACAGGAAATG ACCTTGCTAG GACGCTAAAC TGGGGTGGGG GCTACACAGA TGAACCTCTG 
AGTAAAATCC TGTCTCATGT GGAGGATGGG AATATTGTAC AGCTGGATCG GTGGAATCTT 
GTTGTGAAGC CCAACCCAGA GGCAGGACCA GAGGAAAGAG ACGAGCAGGT GACGGACAAG 
CTCCCTCTAG ACGTCTTCAA TAACTACTTC AGCCTTGGAT TTGATGCTCA TGTGACTTTA 
GAGTTCCACG AATCTCGAGA GGCTAATCCT GAGAAATTTA ACAGCCGGTT CAGAAACAAA 
ATGTTCTATG CAGGGACAGC GTTTTCTGAT TTCTTGATGG GCAGCTCCAA AGACCTGGCT 
AAGCACATCA AAGTAGTCTG TGACGGCACA GATTTAACCT CAAAGGTCCA GGATTTGAAG 
CTGCAATGCC TGGTCTTCCT AAATATCCCC AGGTACTGTG CGGGCACAAT GCCATGGGGC 
AATCCGAGTG AGCATCATGA CTTTGAGCCT CAGCGTCATG ATGATGGCTG CATTGAGGTT 
ATTGGATTCA CAATGACATC ACTGGCCACA CTGCAGGTCG GAGGTCACGG TGAGAGGTTA 
AATCAGTGTC GCGAAGTCAC CCTCACCACC TTTAAGTCAA TCCCCATGCA GGTGGACGGG 
GAGCCTTGTA AACTAGCACC ATCTGTTATT CACATTTCTT TGCGAAACCA GGCCAACATG 
GTGCAGAAAA CCAAGAGACG TACATCTATA CCACTGCTAA ACGATCAGCA ACCGTTTCCA 
GAACGACTAC GGATCAGAGT GAGCCGTATC AGCATGCATG ATTATGAAGC TTTGCACTAC 
GACAAGGAGA AGCTCAAAGA AGCTTCGATT CCACTGGGTC TCATCGTTGT CCCTGGAGAC 
AGTGATTTGG AAACCTGCCG AGCTCACATC GAGAGACTAC AGGAGGAAGG TGACGGGAGC 
AAATCAAAGG CTTTGTCGTC TCAGAGGCTA TCACCGAAAT GGTGCTTTCT CGACTCTACA 
ACTGCTGATC GATTCTACAG GATTGATCGA GCTCAAGAGC ATCTCAACTA CGTGACTGAA 
ATTTCTCAGG ATGAGCTGTT TGTTTTGGAC CCAGAGCTGG TGGTCACAGA GACTGTTAGC 
ACATCACCTG GCATGCCAGA CCTGGTGGAC TCTGGTGAAG TCCAGTCCGA TCCACACAAA 
TTTGCCTTTC CGTCATCCTC TTCTTCACCA CCAAACACAC CTGGACCTAG GGTTGCAGAG 
CTGCAAAGGA AGCGTGTTTC CAGTGACAGT TCGGTGGCAG AAGCACTTGC ACACAGTGAC 
TCGCTCAGAT CAGCCAAGCC AGCTCTCTGC AGAGCTGGGG GTGTTCATCG CTCAAATACA 
ACCGCAGCAG ATTTCAAACC TACCAGCAGG TTTGAGAAGA GCGCTGTGAA CAATGCCAAC 
TCTGTGTCAG TGGACAGTCT AATTGAATGT GTGAAAAAGA AGGACCATCA GAAGTTGAAG 
GAGCTGCATA AGAAAGGGGC CGACCTGTGT GTGCAGGACC CTGCTGGACG GACCCTATTG 
CACTATGCTG TGGAAGTGGG CAGCAAGGAG ATTGTCAAAT ACATCATTGA TAATGCACCC 
ACCGATATCC TGGATGTAAC AGAGAAAGAA AATGGAGAGA CTGTGTTGCA CAAAGCAGCC 
TCGCTGTGTC ATAGGACAAT CTGTCATTAC CTAGTGGAAG CAGGAGCATC TCTTATGAAG 
ACAGACTTAC AGGGTGACAC TCCGAAGCAT CGTGCCGAGA AGGCGAAAGA TGCTGAACTT 
GCGGCATATC TTGAAAACCG CCAGCACTAC CAAATGATCC AGCGTGAGGA CCAGGAGACT 
GCAGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005169091.1
CDS	135..3803
Translation	MDALFNLRFRRKAVEPSGRRRSSVSLPASKARRRSSAGLPSAGLAHRRRSSAQLQIGSIQPGLVLHRRGHFTRRKSSGGTACLTPRFAIRRRQAAKHRSIHAQLLGPSLLLASVVQMSEDHEPEDKSAEEYSSLSDTDCSQDEHEQTGMDFETESDKCLTWLAQGLLVGESIQPRPLIRAPRCLRRNSSHLLPAEAVYRSPASYGLYGRYRRTSQVHNMGGLWAGKRSSLAARGSVALYRTSPPCWPDLSVSHTLQGTYYNPQIDTWSGFLSKAIAKSGLQHLSCQPSAAVLAKGETDREIRNTVDWSESAVYGEHIWFETNVSGDFCYVGEQHCYAKSLQKSVARKKCAACKIVVHSICIEQLEKINFRCKPSFRESGSRNIREPAIVRHHWVHRRRQEGKCKQCGKGFQQKFAFHSKEIVAISCSWCKQAYHNKVTCFMLQQIEEPCSLGAHAAVIVPPTWIIRVRRPQSSLKSSKKKKRTSFKRKSSKKGAEEARWKPFIVRPIPSQLMKPLLVFVNPKSGGNQGAKIIQTFLWYLNPRQVFDLSQGGPQEGLEMYRKVHNLRILACGGDGTVGWILSALDQLQLNPSPAVAVLPLGTGNDLARTLNWGGGYTDEPLSKILSHVEDGNIVQLDRWNLVVKPNPEAGPEERDEQVTDKLPLDVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGTAFSDFLMGSSKDLAKHIKVVCDGTDLTSKVQDLKLQCLVFLNIPRYCAGTMPWGNPSEHHDFEPQRHDDGCIEVIGFTMTSLATLQVGGHGERLNQCREVTLTTFKSIPMQVDGEPCKLAPSVIHISLRNQANMVQKTKRRTSIPLLNDQQPFPERLRIRVSRISMHDYEALHYDKEKLKEASIPLGLIVVPGDSDLETCRAHIERLQEEGDGSKSKALSSQRLSPKWCFLDSTTADRFYRIDRAQEHLNYVTEISQDELFVLDPELVVTETVSTSPGMPDLVDSGEVQSDPHKFAFPSSSSSPPNTPGPRVAELQRKRVSSDSSVAEALAHSDSLRSAKPALCRAGGVHRSNTTAADFKPTSRFEKSAVNNANSVSVDSLIECVKKKDHQKLKELHKKGADLCVQDPAGRTLLHYAVEVGSKEIVKYIIDNAPTDILDVTEKENGETVLHKAASLCHRTICHYLVEAGASLMKTDLQGDTPKHRAEKAKDAELAAYLENRQHYQMIQREDQETAV

Target ORF information:

RefSeq Version	XM_005169034.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005169034.3

ORF Insert Sequence:

ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT 
TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG 
CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA 
GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT 
CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC 
CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC 
CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA 
TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA 
CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC 
CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT 
GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG 
ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC 
AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC 
CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA 
ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG 
ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG 
CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC 
ATTGAACAGC TGGAGAAGAT AAACTTCAGA TGTAAACCGT CTTTCAGGGA GTCAGGATCA 
CGGAACATCC GAGAGCCTGC AATAGTGCGA CACCACTGGG TCCATCGGAG AAGACAAGAG 
GGCAAATGCA AACAGTGTGG AAAGGGGTTT CAGCAGAAAT TTGCCTTTCA CAGTAAAGAG 
ATTGTAGCCA TCAGCTGCTC CTGGTGCAAA CAAGCATATC ATAATAAGGT GACATGCTTT 
ATGCTGCAGC AGATAGAAGA ACCATGTTCA TTAGGAGCTC ATGCCGCTGT AATAGTACCT 
CCTACTTGGA TCATACGGGT TCGCCGACCT CAGTCCTCAC TAAAGTCAAG CAAAAAGAAA 
AAACGGACAT CTTTCAAACG TAAATCCAGC AAGAAAGGAG CAGAGGAGGC ACGATGGAAG 
CCTTTTATAG TGAGACCCAT TCCCTCCCAA CTCATGAAGC CACTGCTAGT GTTTGTAAAT 
CCAAAGAGTG GAGGAAACCA GGGAGCTAAA ATCATCCAGA CCTTTCTTTG GTATCTAAAC 
CCTCGGCAGG TGTTTGATCT AAGTCAAGGA GGTCCTCAGG AAGGCTTGGA AATGTATCGC 
AAGGTTCACA ACTTGAGAAT TTTGGCCTGT GGGGGGGATG GCACGGTGGG CTGGATCTTA 
TCTGCACTTG ATCAGCTGCA ATTAAACCCT TCTCCTGCTG TGGCCGTACT TCCCCTGGGC 
ACAGGAAATG ACCTTGCTAG GACGCTAAAC TGGGGTGGGG GCTACACAGA TGAACCTCTG 
AGTAAAATCC TGTCTCATGT GGAGGATGGG AATATTGTAC AGCTGGATCG GTGGAATCTT 
GTTGTGAAGC CCAACCCAGA GGCAGGACCA GAGGAAAGAG ACGAGCAGGT GACGGACAAG 
CTCCCTCTAG ACGTCTTCAA TAACTACTTC AGCCTTGGAT TTGATGCTCA TGTGACTTTA 
GAGTTCCACG AATCTCGAGA GGCTAATCCT GAGAAATTTA ACAGCCGGTT CAGAAACAAA 
ATGTTCTATG CAGGGACAGC GTTTTCTGAT TTCTTGATGG GCAGCTCCAA AGACCTGGCT 
AAGCACATCA AAGTAGTCTG TGACGGCACA GATTTAACCT CAAAGGTCCA GGATTTGAAG 
CTGCAATGCC TGGTCTTCCT AAATATCCCC AGGTACTGTG CGGGCACAAT GCCATGGGGC 
AATCCGAGTG AGCATCATGA CTTTGAGCCT CAGCGTCATG ATGATGGCTG CATTGAGGTT 
ATTGGATTCA CAATGACATC ACTGGCCACA CTGCAGGTCG GAGGTCACGG TGAGAGGTTA 
AATCAGTGTC GCGAAGTCAC CCTCACCACC TTTAAGTCAA TCCCCATGCA GGTGGACGGG 
GAGCCTTGTA AACTAGCACC ATCTGTTATT CACATTTCTT TGCGAAACCA GGCCAACATG 
GTGCAGAAAA CCAAGAGACG TACATCTATA CCACTGCTAA ACGATCAGCA ACCGTTTCCA 
GAACGACTAC GGATCAGAGT GAGCCGTATC AGCATGCATG ATTATGAAGC TTTGCACTAC 
GACAAGGAGA AGCTCAAAGA AGCTTCGATT CCACTGGGTC TCATCGTTGT CCCTGGAGAC 
AGTGATTTGG AAACCTGCCG AGCTCACATC GAGAGACTAC AGGAGGAAGG TGACGGGAGC 
AAATCAAAGG CTTTGTCGTC TCAGAGGCTA TCACCGAAAT GGTGCTTTCT CGACTCTACA 
ACTGCTGATC GATTCTACAG GATTGATCGA GCTCAAGAGC ATCTCAACTA CGTGACTGAA 
ATTTCTCAGG ATGAGCTGTT TGTTTTGGAC CCAGAGCTGG TGGTCACAGA GACTGTTAGC 
ACATCACCTG GCATGCCAGA CCTGGTGGAC TCTGGTGAAG TCCAGTCCGA TCCACACAAA 
TTTGCCTTTC CGTCATCCTC TTCTTCACCA CCAAACACAC CTGGACCTAG GGTTGCAGAG 
CTGCAAAGGA AGCGTGTTTC CAGTGACAGT TCGGTGGCAG AAGCACTTGC ACACAGTGAC 
TCGCTCAGAT CAGCCAAGCC AGCTCTCTGC AGAGCTGGGG GTGTTCATCG CTCAAATACA 
ACCGCAGCAG ATTTCAAACC TACCAGCAGG TTTGAGAAGA GCGCTGTGAA CAATGCCAAC 
TCTGTGTCAG TGGACAGTCT AATTGAATGT GTGAAAAAGA AGGACCATCA GAAGTTGAAG 
GAGCTGCATA AGAAAGGGGC CGACCTGTGT GTGCAGGACC CTGCTGGACG GACCCTATTG 
CACTATGCTG TGGAAGTGGG CAGCAAGGAG ATTGTCAAAT ACATCATTGA TAATGCACCC 
ACCGATATCC TGGATGTAAC AGAGAAAGAA AATGGAGAGA CTGTGTTGCA CAAAGCAGCC 
TCGCTGTGTC ATAGGACAAT CTGTCATTAC CTAGTGGAAG CAGGAGCATC TCTTATGAAG 
ACAGACTTAC AGGGTGACAC TCCGAAGCAT CGTGCCGAGA AGGCGAAAGA TGCTGAACTT 
GCGGCATATC TTGAAAACCG CCAGCACTAC CAAATGATCC AGCGTGAGGA CCAGGAGACT 
GCAGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dgkza ( XM_005169034.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005169091.1 Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa13282
Clone ID Related Accession (Same CDS sequence)	XM_005169034.3 , XM_005169034.4
Accession Version	XM_005169034.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	diacylglycerol kinase zeta isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005169034.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT 
TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG 
CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA 
GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT 
CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC 
CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC 
CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA 
TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA 
CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC 
CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT 
GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG 
ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC 
AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC 
CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA 
ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG 
ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG 
CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC 
ATTGAACAGC TGGAGAAGAT AAACTTCAGA TGTAAACCGT CTTTCAGGGA GTCAGGATCA 
CGGAACATCC GAGAGCCTGC AATAGTGCGA CACCACTGGG TCCATCGGAG AAGACAAGAG 
GGCAAATGCA AACAGTGTGG AAAGGGGTTT CAGCAGAAAT TTGCCTTTCA CAGTAAAGAG 
ATTGTAGCCA TCAGCTGCTC CTGGTGCAAA CAAGCATATC ATAATAAGGT GACATGCTTT 
ATGCTGCAGC AGATAGAAGA ACCATGTTCA TTAGGAGCTC ATGCCGCTGT AATAGTACCT 
CCTACTTGGA TCATACGGGT TCGCCGACCT CAGTCCTCAC TAAAGTCAAG CAAAAAGAAA 
AAACGGACAT CTTTCAAACG TAAATCCAGC AAGAAAGGAG CAGAGGAGGC ACGATGGAAG 
CCTTTTATAG TGAGACCCAT TCCCTCCCAA CTCATGAAGC CACTGCTAGT GTTTGTAAAT 
CCAAAGAGTG GAGGAAACCA GGGAGCTAAA ATCATCCAGA CCTTTCTTTG GTATCTAAAC 
CCTCGGCAGG TGTTTGATCT AAGTCAAGGA GGTCCTCAGG AAGGCTTGGA AATGTATCGC 
AAGGTTCACA ACTTGAGAAT TTTGGCCTGT GGGGGGGATG GCACGGTGGG CTGGATCTTA 
TCTGCACTTG ATCAGCTGCA ATTAAACCCT TCTCCTGCTG TGGCCGTACT TCCCCTGGGC 
ACAGGAAATG ACCTTGCTAG GACGCTAAAC TGGGGTGGGG GCTACACAGA TGAACCTCTG 
AGTAAAATCC TGTCTCATGT GGAGGATGGG AATATTGTAC AGCTGGATCG GTGGAATCTT 
GTTGTGAAGC CCAACCCAGA GGCAGGACCA GAGGAAAGAG ACGAGCAGGT GACGGACAAG 
CTCCCTCTAG ACGTCTTCAA TAACTACTTC AGCCTTGGAT TTGATGCTCA TGTGACTTTA 
GAGTTCCACG AATCTCGAGA GGCTAATCCT GAGAAATTTA ACAGCCGGTT CAGAAACAAA 
ATGTTCTATG CAGGGACAGC GTTTTCTGAT TTCTTGATGG GCAGCTCCAA AGACCTGGCT 
AAGCACATCA AAGTAGTCTG TGACGGCACA GATTTAACCT CAAAGGTCCA GGATTTGAAG 
CTGCAATGCC TGGTCTTCCT AAATATCCCC AGGTACTGTG CGGGCACAAT GCCATGGGGC 
AATCCGAGTG AGCATCATGA CTTTGAGCCT CAGCGTCATG ATGATGGCTG CATTGAGGTT 
ATTGGATTCA CAATGACATC ACTGGCCACA CTGCAGGTCG GAGGTCACGG TGAGAGGTTA 
AATCAGTGTC GCGAAGTCAC CCTCACCACC TTTAAGTCAA TCCCCATGCA GGTGGACGGG 
GAGCCTTGTA AACTAGCACC ATCTGTTATT CACATTTCTT TGCGAAACCA GGCCAACATG 
GTGCAGAAAA CCAAGAGACG TACATCTATA CCACTGCTAA ACGATCAGCA ACCGTTTCCA 
GAACGACTAC GGATCAGAGT GAGCCGTATC AGCATGCATG ATTATGAAGC TTTGCACTAC 
GACAAGGAGA AGCTCAAAGA AGCTTCGATT CCACTGGGTC TCATCGTTGT CCCTGGAGAC 
AGTGATTTGG AAACCTGCCG AGCTCACATC GAGAGACTAC AGGAGGAAGG TGACGGGAGC 
AAATCAAAGG CTTTGTCGTC TCAGAGGCTA TCACCGAAAT GGTGCTTTCT CGACTCTACA 
ACTGCTGATC GATTCTACAG GATTGATCGA GCTCAAGAGC ATCTCAACTA CGTGACTGAA 
ATTTCTCAGG ATGAGCTGTT TGTTTTGGAC CCAGAGCTGG TGGTCACAGA GACTGTTAGC 
ACATCACCTG GCATGCCAGA CCTGGTGGAC TCTGGTGAAG TCCAGTCCGA TCCACACAAA 
TTTGCCTTTC CGTCATCCTC TTCTTCACCA CCAAACACAC CTGGACCTAG GGTTGCAGAG 
CTGCAAAGGA AGCGTGTTTC CAGTGACAGT TCGGTGGCAG AAGCACTTGC ACACAGTGAC 
TCGCTCAGAT CAGCCAAGCC AGCTCTCTGC AGAGCTGGGG GTGTTCATCG CTCAAATACA 
ACCGCAGCAG ATTTCAAACC TACCAGCAGG TTTGAGAAGA GCGCTGTGAA CAATGCCAAC 
TCTGTGTCAG TGGACAGTCT AATTGAATGT GTGAAAAAGA AGGACCATCA GAAGTTGAAG 
GAGCTGCATA AGAAAGGGGC CGACCTGTGT GTGCAGGACC CTGCTGGACG GACCCTATTG 
CACTATGCTG TGGAAGTGGG CAGCAAGGAG ATTGTCAAAT ACATCATTGA TAATGCACCC 
ACCGATATCC TGGATGTAAC AGAGAAAGAA AATGGAGAGA CTGTGTTGCA CAAAGCAGCC 
TCGCTGTGTC ATAGGACAAT CTGTCATTAC CTAGTGGAAG CAGGAGCATC TCTTATGAAG 
ACAGACTTAC AGGGTGACAC TCCGAAGCAT CGTGCCGAGA AGGCGAAAGA TGCTGAACTT 
GCGGCATATC TTGAAAACCG CCAGCACTAC CAAATGATCC AGCGTGAGGA CCAGGAGACT 
GCAGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005169091.1
CDS	170..3838
Translation	MDALFNLRFRRKAVEPSGRRRSSVSLPASKARRRSSAGLPSAGLAHRRRSSAQLQIGSIQPGLVLHRRGHFTRRKSSGGTACLTPRFAIRRRQAAKHRSIHAQLLGPSLLLASVVQMSEDHEPEDKSAEEYSSLSDTDCSQDEHEQTGMDFETESDKCLTWLAQGLLVGESIQPRPLIRAPRCLRRNSSHLLPAEAVYRSPASYGLYGRYRRTSQVHNMGGLWAGKRSSLAARGSVALYRTSPPCWPDLSVSHTLQGTYYNPQIDTWSGFLSKAIAKSGLQHLSCQPSAAVLAKGETDREIRNTVDWSESAVYGEHIWFETNVSGDFCYVGEQHCYAKSLQKSVARKKCAACKIVVHSICIEQLEKINFRCKPSFRESGSRNIREPAIVRHHWVHRRRQEGKCKQCGKGFQQKFAFHSKEIVAISCSWCKQAYHNKVTCFMLQQIEEPCSLGAHAAVIVPPTWIIRVRRPQSSLKSSKKKKRTSFKRKSSKKGAEEARWKPFIVRPIPSQLMKPLLVFVNPKSGGNQGAKIIQTFLWYLNPRQVFDLSQGGPQEGLEMYRKVHNLRILACGGDGTVGWILSALDQLQLNPSPAVAVLPLGTGNDLARTLNWGGGYTDEPLSKILSHVEDGNIVQLDRWNLVVKPNPEAGPEERDEQVTDKLPLDVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGTAFSDFLMGSSKDLAKHIKVVCDGTDLTSKVQDLKLQCLVFLNIPRYCAGTMPWGNPSEHHDFEPQRHDDGCIEVIGFTMTSLATLQVGGHGERLNQCREVTLTTFKSIPMQVDGEPCKLAPSVIHISLRNQANMVQKTKRRTSIPLLNDQQPFPERLRIRVSRISMHDYEALHYDKEKLKEASIPLGLIVVPGDSDLETCRAHIERLQEEGDGSKSKALSSQRLSPKWCFLDSTTADRFYRIDRAQEHLNYVTEISQDELFVLDPELVVTETVSTSPGMPDLVDSGEVQSDPHKFAFPSSSSSPPNTPGPRVAELQRKRVSSDSSVAEALAHSDSLRSAKPALCRAGGVHRSNTTAADFKPTSRFEKSAVNNANSVSVDSLIECVKKKDHQKLKELHKKGADLCVQDPAGRTLLHYAVEVGSKEIVKYIIDNAPTDILDVTEKENGETVLHKAASLCHRTICHYLVEAGASLMKTDLQGDTPKHRAEKAKDAELAAYLENRQHYQMIQREDQETAV

Target ORF information:

RefSeq Version	XM_005169034.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005169034.4

ORF Insert Sequence:

ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT 
TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG 
CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA 
GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT 
CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC 
CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC 
CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA 
TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA 
CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC 
CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT 
GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG 
ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC 
AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC 
CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA 
ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG 
ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG 
CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC 
ATTGAACAGC TGGAGAAGAT AAACTTCAGA TGTAAACCGT CTTTCAGGGA GTCAGGATCA 
CGGAACATCC GAGAGCCTGC AATAGTGCGA CACCACTGGG TCCATCGGAG AAGACAAGAG 
GGCAAATGCA AACAGTGTGG AAAGGGGTTT CAGCAGAAAT TTGCCTTTCA CAGTAAAGAG 
ATTGTAGCCA TCAGCTGCTC CTGGTGCAAA CAAGCATATC ATAATAAGGT GACATGCTTT 
ATGCTGCAGC AGATAGAAGA ACCATGTTCA TTAGGAGCTC ATGCCGCTGT AATAGTACCT 
CCTACTTGGA TCATACGGGT TCGCCGACCT CAGTCCTCAC TAAAGTCAAG CAAAAAGAAA 
AAACGGACAT CTTTCAAACG TAAATCCAGC AAGAAAGGAG CAGAGGAGGC ACGATGGAAG 
CCTTTTATAG TGAGACCCAT TCCCTCCCAA CTCATGAAGC CACTGCTAGT GTTTGTAAAT 
CCAAAGAGTG GAGGAAACCA GGGAGCTAAA ATCATCCAGA CCTTTCTTTG GTATCTAAAC 
CCTCGGCAGG TGTTTGATCT AAGTCAAGGA GGTCCTCAGG AAGGCTTGGA AATGTATCGC 
AAGGTTCACA ACTTGAGAAT TTTGGCCTGT GGGGGGGATG GCACGGTGGG CTGGATCTTA 
TCTGCACTTG ATCAGCTGCA ATTAAACCCT TCTCCTGCTG TGGCCGTACT TCCCCTGGGC 
ACAGGAAATG ACCTTGCTAG GACGCTAAAC TGGGGTGGGG GCTACACAGA TGAACCTCTG 
AGTAAAATCC TGTCTCATGT GGAGGATGGG AATATTGTAC AGCTGGATCG GTGGAATCTT 
GTTGTGAAGC CCAACCCAGA GGCAGGACCA GAGGAAAGAG ACGAGCAGGT GACGGACAAG 
CTCCCTCTAG ACGTCTTCAA TAACTACTTC AGCCTTGGAT TTGATGCTCA TGTGACTTTA 
GAGTTCCACG AATCTCGAGA GGCTAATCCT GAGAAATTTA ACAGCCGGTT CAGAAACAAA 
ATGTTCTATG CAGGGACAGC GTTTTCTGAT TTCTTGATGG GCAGCTCCAA AGACCTGGCT 
AAGCACATCA AAGTAGTCTG TGACGGCACA GATTTAACCT CAAAGGTCCA GGATTTGAAG 
CTGCAATGCC TGGTCTTCCT AAATATCCCC AGGTACTGTG CGGGCACAAT GCCATGGGGC 
AATCCGAGTG AGCATCATGA CTTTGAGCCT CAGCGTCATG ATGATGGCTG CATTGAGGTT 
ATTGGATTCA CAATGACATC ACTGGCCACA CTGCAGGTCG GAGGTCACGG TGAGAGGTTA 
AATCAGTGTC GCGAAGTCAC CCTCACCACC TTTAAGTCAA TCCCCATGCA GGTGGACGGG 
GAGCCTTGTA AACTAGCACC ATCTGTTATT CACATTTCTT TGCGAAACCA GGCCAACATG 
GTGCAGAAAA CCAAGAGACG TACATCTATA CCACTGCTAA ACGATCAGCA ACCGTTTCCA 
GAACGACTAC GGATCAGAGT GAGCCGTATC AGCATGCATG ATTATGAAGC TTTGCACTAC 
GACAAGGAGA AGCTCAAAGA AGCTTCGATT CCACTGGGTC TCATCGTTGT CCCTGGAGAC 
AGTGATTTGG AAACCTGCCG AGCTCACATC GAGAGACTAC AGGAGGAAGG TGACGGGAGC 
AAATCAAAGG CTTTGTCGTC TCAGAGGCTA TCACCGAAAT GGTGCTTTCT CGACTCTACA 
ACTGCTGATC GATTCTACAG GATTGATCGA GCTCAAGAGC ATCTCAACTA CGTGACTGAA 
ATTTCTCAGG ATGAGCTGTT TGTTTTGGAC CCAGAGCTGG TGGTCACAGA GACTGTTAGC 
ACATCACCTG GCATGCCAGA CCTGGTGGAC TCTGGTGAAG TCCAGTCCGA TCCACACAAA 
TTTGCCTTTC CGTCATCCTC TTCTTCACCA CCAAACACAC CTGGACCTAG GGTTGCAGAG 
CTGCAAAGGA AGCGTGTTTC CAGTGACAGT TCGGTGGCAG AAGCACTTGC ACACAGTGAC 
TCGCTCAGAT CAGCCAAGCC AGCTCTCTGC AGAGCTGGGG GTGTTCATCG CTCAAATACA 
ACCGCAGCAG ATTTCAAACC TACCAGCAGG TTTGAGAAGA GCGCTGTGAA CAATGCCAAC 
TCTGTGTCAG TGGACAGTCT AATTGAATGT GTGAAAAAGA AGGACCATCA GAAGTTGAAG 
GAGCTGCATA AGAAAGGGGC CGACCTGTGT GTGCAGGACC CTGCTGGACG GACCCTATTG 
CACTATGCTG TGGAAGTGGG CAGCAAGGAG ATTGTCAAAT ACATCATTGA TAATGCACCC 
ACCGATATCC TGGATGTAAC AGAGAAAGAA AATGGAGAGA CTGTGTTGCA CAAAGCAGCC 
TCGCTGTGTC ATAGGACAAT CTGTCATTAC CTAGTGGAAG CAGGAGCATC TCTTATGAAG 
ACAGACTTAC AGGGTGACAC TCCGAAGCAT CGTGCCGAGA AGGCGAAAGA TGCTGAACTT 
GCGGCATATC TTGAAAACCG CCAGCACTAC CAAATGATCC AGCGTGAGGA CCAGGAGACT 
GCAGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dgkza ( XM_005169035.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005169092.1 Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X9, mRNA.

Price & Availability↑

CloneID	ODa13283
Clone ID Related Accession (Same CDS sequence)	XM_005169035.3
Accession Version	XM_005169035.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	diacylglycerol kinase zeta isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169035.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGA AATTGGACGC GACCGTTGAC GGTTCGCCAC GCGCCGCGCA CAGCTCCGCG 
GACTCGCCGA CCACCGTCCT GATTCCGCCT GCCCTGGAGG ACGTGGTGGA GGAAGAGGAC 
GTGTTCCTCT CTGAAACCGG GCACAGCGGA GCAACTTCAA CACCGCACGA GTGTCCGGGG 
AACGGGGTTG AGAGACTGGC GGAGGGGAGA GAAGGAGAAG TGCGTCCTGA CAACCAGGCG 
GAGAGCAGCG AGTTCCTGCA CCTCACTATC CGGAAACAGG TGTCATACAG GAAGGCTATT 
GCCAAGTCCG GTCTCCAGCA CCTCTCATGC CAGCCCAGTG CTGCAGTGTT AGCCAAAGGG 
GAAACGGACA GAGAAATACG CAACACAGTA GACTGGAGTG AATCAGCTGT ATACGGAGAG 
CACATCTGGT TTGAGACGAA TGTCTCGGGG GACTTCTGTT ACGTTGGAGA ACAGCACTGT 
TACGCCAAGT CACTGCAAAA GTCTGTTGCT CGGAAAAAGT GTGCGGCTTG CAAGATCGTA 
GTTCACTCTA TCTGCATTGA ACAGCTGGAG AAGTTATTGG ATTGGAATGC TCCACCTCAG 
GGGGAGGGGC TTGATGAGAT GCCTCTCAAA GAGCCGTCAT TCAGAATAAA CTTCAGATGT 
AAACCGTCTT TCAGGGAGTC AGGATCACGG AACATCCGAG AGCCTGCAAT AGTGCGACAC 
CACTGGGTCC ATCGGAGAAG ACAAGAGGGC AAATGCAAAC AGTGTGGAAA GGGGTTTCAG 
CAGAAATTTG CCTTTCACAG TAAAGAGATT GTAGCCATCA GCTGCTCCTG GTGCAAACAA 
GCATATCATA ATAAGGTGAC ATGCTTTATG CTGCAGCAGA TAGAAGAACC ATGTTCATTA 
GGAGCTCATG CCGCTGTAAT AGTACCTCCT ACTTGGATCA TACGGGTTCG CCGACCTCAG 
TCCTCACTAA AGTCAAGCAA AAAGAAAAAA CGGACATCTT TCAAACGTAA ATCCAGCAAG 
AAAGGAGCAG AGGAGGCACG ATGGAAGCCT TTTATAGTGA GACCCATTCC CTCCCAACTC 
ATGAAGCCAC TGCTAGTGTT TGTAAATCCA AAGAGTGGAG GAAACCAGGG AGCTAAAATC 
ATCCAGACCT TTCTTTGGTA TCTAAACCCT CGGCAGGTGT TTGATCTAAG TCAAGGAGGT 
CCTCAGGAAG GCTTGGAAAT GTATCGCAAG GTTCACAACT TGAGAATTTT GGCCTGTGGG 
GGGGATGGCA CGGTGGGCTG GATCTTATCT GCACTTGATC AGCTGCAATT AAACCCTTCT 
CCTGCTGTGG CCGTACTTCC CCTGGGCACA GGAAATGACC TTGCTAGGAC GCTAAACTGG 
GGTGGGGGCT ACACAGATGA ACCTCTGAGT AAAATCCTGT CTCATGTGGA GGATGGGAAT 
ATTGTACAGC TGGATCGGTG GAATCTTGTT GTGAAGCCCA ACCCAGAGGC AGGACCAGAG 
GAAAGAGACG AGCAGGTGAC GGACAAGCTC CCTCTAGACG TCTTCAATAA CTACTTCAGC 
CTTGGATTTG ATGCTCATGT GACTTTAGAG TTCCACGAAT CTCGAGAGGC TAATCCTGAG 
AAATTTAACA GCCGGTTCAG AAACAAAATG TTCTATGCAG GGACAGCGTT TTCTGATTTC 
TTGATGGGCA GCTCCAAAGA CCTGGCTAAG CACATCAAAG TAGTCTGTGA CGGCACAGAT 
TTAACCTCAA AGGTCCAGGA TTTGAAGCTG CAATGCCTGG TCTTCCTAAA TATCCCCAGG 
TACTGTGCGG GCACAATGCC ATGGGGCAAT CCGAGTGAGC ATCATGACTT TGAGCCTCAG 
CGTCATGATG ATGGCTGCAT TGAGGTTATT GGATTCACAA TGACATCACT GGCCACACTG 
CAGGTCGGAG GTCACGGTGA GAGGTTAAAT CAGTGTCGCG AAGTCACCCT CACCACCTTT 
AAGTCAATCC CCATGCAGGT GGACGGGGAG CCTTGTAAAC TAGCACCATC TGTTATTCAC 
ATTTCTTTGC GAAACCAGGC CAACATGGTG CAGAAAACCA AGAGACGTAC ATCTATACCA 
CTGCTAAACG ACAGTCAGCA ACCGTTTCCA GAACGACTAC GGATCAGAGT GAGCCGTATC 
AGCATGCATG ATTATGAAGC TTTGCACTAC GACAAGGAGA AGCTCAAAGA AGCTTCGATT 
CCACTGGGTC TCATCGTTGT CCCTGGAGAC AGTGATTTGG AAACCTGCCG AGCTCACATC 
GAGAGACTAC AGGAGGACTT TGTGTCGTGT AACCCGTCTC TGCAGCGGCT GGTGCTCCAG 
GAAGGTGACG GGAGCAAATC AAAGGCTTTG TCGTCTCAGA GGCTATCACC GAAATGGTGC 
TTTCTCGACT CTACAACTGC TGATCGATTC TACAGGATTG ATCGAGCTCA AGAGCATCTC 
AACTACGTGA CTGAAATTTC TCAGGATGAG CTGTTTGTTT TGGACCCAGA GCTGGTGGTC 
ACAGAGACTG TTAGCACATC ACCTGGCATG CCAGACCTGG TGGACTCTGG TGAAGTCCAG 
TCCGATCCAC ACAAATTTGC CTTTCCGTCA TCCTCTTCTT CACCACCAAA CACACCTGGA 
CCTAGGGTTG CAGAGCTGCA AAGGAAGCGT GTTTCCAGTG ACAGTTCGGT GGCAGAAGCA 
CTTGCACACA GTGACTCGCT CAGATCAGCC AAGCCAGCTC TCTGCAGAGC TGGGGGTGTT 
CATCGCTCAA ATACAACCGC AGCAGATTTC AAACCTACCA GCAGGTTTGA GAAGAGCGCT 
GTGAACAATG CCAACTCTGT GTCAGTGGAC AGTCTAATTG AATGTGTGAA AAAGAAGGAC 
CATCAGAAGT TGAAGGAGCT GCATAAGAAA GGGGCCGACC TGTGTGTGCA GGACCCTGCT 
GGACGGACCC TATTGCACTA TGCTGTGGAA GTGGGCAGCA AGGAGATTGT CAAATACATC 
ATTGATAATG CACCCACCGA TATCCTGGAT GTAACAGAGA AAGAAAATGG AGAGACTGTG 
TTGCACAAAG CAGCCTCGCT GTGTCATAGG ACAATCTGTC ATTACCTAGT GGAAGCAGGA 
GCATCTCTTA TGAAGACAGA CTTACAGGGT GACACTCCGA AGCATCGTGC CGAGAAGGCG 
AAAGATGCTG AACTTGCGGC ATATCTTGAA AACCGCCAGC ACTACCAAAT GATCCAGCGT 
GAGGACCAGG AGACTGCAGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005169092.1
CDS	236..3499
Translation	MAEKLDATVDGSPRAAHSSADSPTTVLIPPALEDVVEEEDVFLSETGHSGATSTPHECPGNGVERLAEGREGEVRPDNQAESSEFLHLTIRKQVSYRKAIAKSGLQHLSCQPSAAVLAKGETDREIRNTVDWSESAVYGEHIWFETNVSGDFCYVGEQHCYAKSLQKSVARKKCAACKIVVHSICIEQLEKLLDWNAPPQGEGLDEMPLKEPSFRINFRCKPSFRESGSRNIREPAIVRHHWVHRRRQEGKCKQCGKGFQQKFAFHSKEIVAISCSWCKQAYHNKVTCFMLQQIEEPCSLGAHAAVIVPPTWIIRVRRPQSSLKSSKKKKRTSFKRKSSKKGAEEARWKPFIVRPIPSQLMKPLLVFVNPKSGGNQGAKIIQTFLWYLNPRQVFDLSQGGPQEGLEMYRKVHNLRILACGGDGTVGWILSALDQLQLNPSPAVAVLPLGTGNDLARTLNWGGGYTDEPLSKILSHVEDGNIVQLDRWNLVVKPNPEAGPEERDEQVTDKLPLDVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGTAFSDFLMGSSKDLAKHIKVVCDGTDLTSKVQDLKLQCLVFLNIPRYCAGTMPWGNPSEHHDFEPQRHDDGCIEVIGFTMTSLATLQVGGHGERLNQCREVTLTTFKSIPMQVDGEPCKLAPSVIHISLRNQANMVQKTKRRTSIPLLNDSQQPFPERLRIRVSRISMHDYEALHYDKEKLKEASIPLGLIVVPGDSDLETCRAHIERLQEDFVSCNPSLQRLVLQEGDGSKSKALSSQRLSPKWCFLDSTTADRFYRIDRAQEHLNYVTEISQDELFVLDPELVVTETVSTSPGMPDLVDSGEVQSDPHKFAFPSSSSSPPNTPGPRVAELQRKRVSSDSSVAEALAHSDSLRSAKPALCRAGGVHRSNTTAADFKPTSRFEKSAVNNANSVSVDSLIECVKKKDHQKLKELHKKGADLCVQDPAGRTLLHYAVEVGSKEIVKYIIDNAPTDILDVTEKENGETVLHKAASLCHRTICHYLVEAGASLMKTDLQGDTPKHRAEKAKDAELAAYLENRQHYQMIQREDQETAV

Target ORF information:

RefSeq Version	XM_005169035.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005169035.3

ORF Insert Sequence:

ATGGCAGAGA AATTGGACGC GACCGTTGAC GGTTCGCCAC GCGCCGCGCA CAGCTCCGCG 
GACTCGCCGA CCACCGTCCT GATTCCGCCT GCCCTGGAGG ACGTGGTGGA GGAAGAGGAC 
GTGTTCCTCT CTGAAACCGG GCACAGCGGA GCAACTTCAA CACCGCACGA GTGTCCGGGG 
AACGGGGTTG AGAGACTGGC GGAGGGGAGA GAAGGAGAAG TGCGTCCTGA CAACCAGGCG 
GAGAGCAGCG AGTTCCTGCA CCTCACTATC CGGAAACAGG TGTCATACAG GAAGGCTATT 
GCCAAGTCCG GTCTCCAGCA CCTCTCATGC CAGCCCAGTG CTGCAGTGTT AGCCAAAGGG 
GAAACGGACA GAGAAATACG CAACACAGTA GACTGGAGTG AATCAGCTGT ATACGGAGAG 
CACATCTGGT TTGAGACGAA TGTCTCGGGG GACTTCTGTT ACGTTGGAGA ACAGCACTGT 
TACGCCAAGT CACTGCAAAA GTCTGTTGCT CGGAAAAAGT GTGCGGCTTG CAAGATCGTA 
GTTCACTCTA TCTGCATTGA ACAGCTGGAG AAGTTATTGG ATTGGAATGC TCCACCTCAG 
GGGGAGGGGC TTGATGAGAT GCCTCTCAAA GAGCCGTCAT TCAGAATAAA CTTCAGATGT 
AAACCGTCTT TCAGGGAGTC AGGATCACGG AACATCCGAG AGCCTGCAAT AGTGCGACAC 
CACTGGGTCC ATCGGAGAAG ACAAGAGGGC AAATGCAAAC AGTGTGGAAA GGGGTTTCAG 
CAGAAATTTG CCTTTCACAG TAAAGAGATT GTAGCCATCA GCTGCTCCTG GTGCAAACAA 
GCATATCATA ATAAGGTGAC ATGCTTTATG CTGCAGCAGA TAGAAGAACC ATGTTCATTA 
GGAGCTCATG CCGCTGTAAT AGTACCTCCT ACTTGGATCA TACGGGTTCG CCGACCTCAG 
TCCTCACTAA AGTCAAGCAA AAAGAAAAAA CGGACATCTT TCAAACGTAA ATCCAGCAAG 
AAAGGAGCAG AGGAGGCACG ATGGAAGCCT TTTATAGTGA GACCCATTCC CTCCCAACTC 
ATGAAGCCAC TGCTAGTGTT TGTAAATCCA AAGAGTGGAG GAAACCAGGG AGCTAAAATC 
ATCCAGACCT TTCTTTGGTA TCTAAACCCT CGGCAGGTGT TTGATCTAAG TCAAGGAGGT 
CCTCAGGAAG GCTTGGAAAT GTATCGCAAG GTTCACAACT TGAGAATTTT GGCCTGTGGG 
GGGGATGGCA CGGTGGGCTG GATCTTATCT GCACTTGATC AGCTGCAATT AAACCCTTCT 
CCTGCTGTGG CCGTACTTCC CCTGGGCACA GGAAATGACC TTGCTAGGAC GCTAAACTGG 
GGTGGGGGCT ACACAGATGA ACCTCTGAGT AAAATCCTGT CTCATGTGGA GGATGGGAAT 
ATTGTACAGC TGGATCGGTG GAATCTTGTT GTGAAGCCCA ACCCAGAGGC AGGACCAGAG 
GAAAGAGACG AGCAGGTGAC GGACAAGCTC CCTCTAGACG TCTTCAATAA CTACTTCAGC 
CTTGGATTTG ATGCTCATGT GACTTTAGAG TTCCACGAAT CTCGAGAGGC TAATCCTGAG 
AAATTTAACA GCCGGTTCAG AAACAAAATG TTCTATGCAG GGACAGCGTT TTCTGATTTC 
TTGATGGGCA GCTCCAAAGA CCTGGCTAAG CACATCAAAG TAGTCTGTGA CGGCACAGAT 
TTAACCTCAA AGGTCCAGGA TTTGAAGCTG CAATGCCTGG TCTTCCTAAA TATCCCCAGG 
TACTGTGCGG GCACAATGCC ATGGGGCAAT CCGAGTGAGC ATCATGACTT TGAGCCTCAG 
CGTCATGATG ATGGCTGCAT TGAGGTTATT GGATTCACAA TGACATCACT GGCCACACTG 
CAGGTCGGAG GTCACGGTGA GAGGTTAAAT CAGTGTCGCG AAGTCACCCT CACCACCTTT 
AAGTCAATCC CCATGCAGGT GGACGGGGAG CCTTGTAAAC TAGCACCATC TGTTATTCAC 
ATTTCTTTGC GAAACCAGGC CAACATGGTG CAGAAAACCA AGAGACGTAC ATCTATACCA 
CTGCTAAACG ACAGTCAGCA ACCGTTTCCA GAACGACTAC GGATCAGAGT GAGCCGTATC 
AGCATGCATG ATTATGAAGC TTTGCACTAC GACAAGGAGA AGCTCAAAGA AGCTTCGATT 
CCACTGGGTC TCATCGTTGT CCCTGGAGAC AGTGATTTGG AAACCTGCCG AGCTCACATC 
GAGAGACTAC AGGAGGACTT TGTGTCGTGT AACCCGTCTC TGCAGCGGCT GGTGCTCCAG 
GAAGGTGACG GGAGCAAATC AAAGGCTTTG TCGTCTCAGA GGCTATCACC GAAATGGTGC 
TTTCTCGACT CTACAACTGC TGATCGATTC TACAGGATTG ATCGAGCTCA AGAGCATCTC 
AACTACGTGA CTGAAATTTC TCAGGATGAG CTGTTTGTTT TGGACCCAGA GCTGGTGGTC 
ACAGAGACTG TTAGCACATC ACCTGGCATG CCAGACCTGG TGGACTCTGG TGAAGTCCAG 
TCCGATCCAC ACAAATTTGC CTTTCCGTCA TCCTCTTCTT CACCACCAAA CACACCTGGA 
CCTAGGGTTG CAGAGCTGCA AAGGAAGCGT GTTTCCAGTG ACAGTTCGGT GGCAGAAGCA 
CTTGCACACA GTGACTCGCT CAGATCAGCC AAGCCAGCTC TCTGCAGAGC TGGGGGTGTT 
CATCGCTCAA ATACAACCGC AGCAGATTTC AAACCTACCA GCAGGTTTGA GAAGAGCGCT 
GTGAACAATG CCAACTCTGT GTCAGTGGAC AGTCTAATTG AATGTGTGAA AAAGAAGGAC 
CATCAGAAGT TGAAGGAGCT GCATAAGAAA GGGGCCGACC TGTGTGTGCA GGACCCTGCT 
GGACGGACCC TATTGCACTA TGCTGTGGAA GTGGGCAGCA AGGAGATTGT CAAATACATC 
ATTGATAATG CACCCACCGA TATCCTGGAT GTAACAGAGA AAGAAAATGG AGAGACTGTG 
TTGCACAAAG CAGCCTCGCT GTGTCATAGG ACAATCTGTC ATTACCTAGT GGAAGCAGGA 
GCATCTCTTA TGAAGACAGA CTTACAGGGT GACACTCCGA AGCATCGTGC CGAGAAGGCG 
AAAGATGCTG AACTTGCGGC ATATCTTGAA AACCGCCAGC ACTACCAAAT GATCCAGCGT 
GAGGACCAGG AGACTGCAGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dgkza ( XM_001922410.6 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001922445.3 Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa13284
Clone ID Related Accession (Same CDS sequence)	XM_001922410.6 , XM_001922410.5
Accession Version	XM_001922410.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	diacylglycerol kinase zeta isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001922410.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGA AATTGGACGC GACCGTTGAC GGTTCGCCAC GCGCCGCGCA CAGCTCCGCG 
GACTCGCCGA CCACCGTCCT GATTCCGCCT GCCCTGGAGG ACGTGGTGGA GGAAGAGGAC 
GTGTTCCTCT CTGAAACCGG GCACAGCGGA GCAACTTCAA CACCGCACGA GTGTCCGGGG 
AACGGGGTTG AGAGACTGGC GGAGGGGAGA GAAGGAGAAG TGCGTCCTGA CAACCAGGCG 
GAGAGCAGCG AGTTCCTGCA CCTCACTATC CGGAAACAGG TGTCATACAG GAAGGCTATT 
GCCAAGTCCG GTCTCCAGCA CCTCTCATGC CAGCCCAGTG CTGCAGTGTT AGCCAAAGGG 
GAAACGGACA GAGAAATACG CAACACAGTA GACTGGAGTG AATCAGCTGT ATACGGAGAG 
CACATCTGGT TTGAGACGAA TGTCTCGGGG GACTTCTGTT ACGTTGGAGA ACAGCACTGT 
TACGCCAAGT CACTGCAAAA GTCTGTTGCT CGGAAAAAGT GTGCGGCTTG CAAGATCGTA 
GTTCACTCTA TCTGCATTGA ACAGCTGGAG AAGATAAACT TCAGATGTAA ACCGTCTTTC 
AGGGAGTCAG GATCACGGAA CATCCGAGAG CCTGCAATAG TGCGACACCA CTGGGTCCAT 
CGGAGAAGAC AAGAGGGCAA ATGCAAACAG TGTGGAAAGG GGTTTCAGCA GAAATTTGCC 
TTTCACAGTA AAGAGATTGT AGCCATCAGC TGCTCCTGGT GCAAACAAGC ATATCATAAT 
AAGGTGACAT GCTTTATGCT GCAGCAGATA GAAGAACCAT GTTCATTAGG AGCTCATGCC 
GCTGTAATAG TACCTCCTAC TTGGATCATA CGGGTTCGCC GACCTCAGTC CTCACTAAAG 
TCAAGCAAAA AGAAAAAACG GACATCTTTC AAACGTAAAT CCAGCAAGAA AGGAGCAGAG 
GAGGCACGAT GGAAGCCTTT TATAGTGAGA CCCATTCCCT CCCAACTCAT GAAGCCACTG 
CTAGTGTTTG TAAATCCAAA GAGTGGAGGA AACCAGGGAG CTAAAATCAT CCAGACCTTT 
CTTTGGTATC TAAACCCTCG GCAGGTGTTT GATCTAAGTC AAGGAGGTCC TCAGGAAGGC 
TTGGAAATGT ATCGCAAGGT TCACAACTTG AGAATTTTGG CCTGTGGGGG GGATGGCACG 
GTGGGCTGGA TCTTATCTGC ACTTGATCAG CTGCAATTAA ACCCTTCTCC TGCTGTGGCC 
GTACTTCCCC TGGGCACAGG AAATGACCTT GCTAGGACGC TAAACTGGGG TGGGGGCTAC 
ACAGATGAAC CTCTGAGTAA AATCCTGTCT CATGTGGAGG ATGGGAATAT TGTACAGCTG 
GATCGGTGGA ATCTTGTTGT GAAGCCCAAC CCAGAGGCAG GACCAGAGGA AAGAGACGAG 
CAGGTGACGG ACAAGCTCCC TCTAGACGTC TTCAATAACT ACTTCAGCCT TGGATTTGAT 
GCTCATGTGA CTTTAGAGTT CCACGAATCT CGAGAGGCTA ATCCTGAGAA ATTTAACAGC 
CGGTTCAGAA ACAAAATGTT CTATGCAGGG ACAGCGTTTT CTGATTTCTT GATGGGCAGC 
TCCAAAGACC TGGCTAAGCA CATCAAAGTA GTCTGTGACG GCACAGATTT AACCTCAAAG 
GTCCAGGATT TGAAGCTGCA ATGCCTGGTC TTCCTAAATA TCCCCAGGTA CTGTGCGGGC 
ACAATGCCAT GGGGCAATCC GAGTGAGCAT CATGACTTTG AGCCTCAGCG TCATGATGAT 
GGCTGCATTG AGGTTATTGG ATTCACAATG ACATCACTGG CCACACTGCA GGTCGGAGGT 
CACGGTGAGA GGTTAAATCA GTGTCGCGAA GTCACCCTCA CCACCTTTAA GTCAATCCCC 
ATGCAGGTGG ACGGGGAGCC TTGTAAACTA GCACCATCTG TTATTCACAT TTCTTTGCGA 
AACCAGGCCA ACATGGTGCA GAAAACCAAG AGACGTACAT CTATACCACT GCTAAACGAC 
AGTCAGCAAC CGTTTCCAGA ACGACTACGG ATCAGAGTGA GCCGTATCAG CATGCATGAT 
TATGAAGCTT TGCACTACGA CAAGGAGAAG CTCAAAGAAG CTTCGATTCC ACTGGGTCTC 
ATCGTTGTCC CTGGAGACAG TGATTTGGAA ACCTGCCGAG CTCACATCGA GAGACTACAG 
GAGGACTTTG TGTCGTGTAA CCCGTCTCTG CAGCGGCTGG TGCTCCAGGA AGGTGACGGG 
AGCAAATCAA AGGCTTTGTC GTCTCAGAGG CTATCACCGA AATGGTGCTT TCTCGACTCT 
ACAACTGCTG ATCGATTCTA CAGGATTGAT CGAGCTCAAG AGCATCTCAA CTACGTGACT 
GAAATTTCTC AGGATGAGCT GTTTGTTTTG GACCCAGAGC TGGTGGTCAC AGAGACTGTT 
AGCACATCAC CTGGCATGCC AGACCTGGTG GACTCTGGTG AAGTCCAGTC CGATCCACAC 
AAATTTGCCT TTCCGTCATC CTCTTCTTCA CCACCAAACA CACCTGGACC TAGGGTTGCA 
GAGCTGCAAA GGAAGCGTGT TTCCAGTGAC AGTTCGGTGG CAGAAGCACT TGCACACAGT 
GACTCGCTCA GATCAGCCAA GCCAGCTCTC TGCAGAGCTG GGGGTGTTCA TCGCTCAAAT 
ACAACCGCAG CAGATTTCAA ACCTACCAGC AGGTTTGAGA AGAGCGCTGT GAACAATGCC 
AACTCTGTGT CAGTGGACAG TCTAATTGAA TGTGTGAAAA AGAAGGACCA TCAGAAGTTG 
AAGGAGCTGC ATAAGAAAGG GGCCGACCTG TGTGTGCAGG ACCCTGCTGG ACGGACCCTA 
TTGCACTATG CTGTGGAAGT GGGCAGCAAG GAGATTGTCA AATACATCAT TGATAATGCA 
CCCACCGATA TCCTGGATGT AACAGAGAAA GAAAATGGAG AGACTGTGTT GCACAAAGCA 
GCCTCGCTGT GTCATAGGAC AATCTGTCAT TACCTAGTGG AAGCAGGAGC ATCTCTTATG 
AAGACAGACT TACAGGGTGA CACTCCGAAG CATCGTGCCG AGAAGGCGAA AGATGCTGAA 
CTTGCGGCAT ATCTTGAAAA CCGCCAGCAC TACCAAATGA TCCAGCGTGA GGACCAGGAG 
ACTGCAGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001922445.3
CDS	236..3427
Translation	MAEKLDATVDGSPRAAHSSADSPTTVLIPPALEDVVEEEDVFLSETGHSGATSTPHECPGNGVERLAEGREGEVRPDNQAESSEFLHLTIRKQVSYRKAIAKSGLQHLSCQPSAAVLAKGETDREIRNTVDWSESAVYGEHIWFETNVSGDFCYVGEQHCYAKSLQKSVARKKCAACKIVVHSICIEQLEKINFRCKPSFRESGSRNIREPAIVRHHWVHRRRQEGKCKQCGKGFQQKFAFHSKEIVAISCSWCKQAYHNKVTCFMLQQIEEPCSLGAHAAVIVPPTWIIRVRRPQSSLKSSKKKKRTSFKRKSSKKGAEEARWKPFIVRPIPSQLMKPLLVFVNPKSGGNQGAKIIQTFLWYLNPRQVFDLSQGGPQEGLEMYRKVHNLRILACGGDGTVGWILSALDQLQLNPSPAVAVLPLGTGNDLARTLNWGGGYTDEPLSKILSHVEDGNIVQLDRWNLVVKPNPEAGPEERDEQVTDKLPLDVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGTAFSDFLMGSSKDLAKHIKVVCDGTDLTSKVQDLKLQCLVFLNIPRYCAGTMPWGNPSEHHDFEPQRHDDGCIEVIGFTMTSLATLQVGGHGERLNQCREVTLTTFKSIPMQVDGEPCKLAPSVIHISLRNQANMVQKTKRRTSIPLLNDSQQPFPERLRIRVSRISMHDYEALHYDKEKLKEASIPLGLIVVPGDSDLETCRAHIERLQEDFVSCNPSLQRLVLQEGDGSKSKALSSQRLSPKWCFLDSTTADRFYRIDRAQEHLNYVTEISQDELFVLDPELVVTETVSTSPGMPDLVDSGEVQSDPHKFAFPSSSSSPPNTPGPRVAELQRKRVSSDSSVAEALAHSDSLRSAKPALCRAGGVHRSNTTAADFKPTSRFEKSAVNNANSVSVDSLIECVKKKDHQKLKELHKKGADLCVQDPAGRTLLHYAVEVGSKEIVKYIIDNAPTDILDVTEKENGETVLHKAASLCHRTICHYLVEAGASLMKTDLQGDTPKHRAEKAKDAELAAYLENRQHYQMIQREDQETAV

Target ORF information:

RefSeq Version	XM_001922410.6
Organism	Danio rerio(zebrafish)
Definition	Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001922410.6

ORF Insert Sequence:

ATGGCAGAGA AATTGGACGC GACCGTTGAC GGTTCGCCAC GCGCCGCGCA CAGCTCCGCG 
GACTCGCCGA CCACCGTCCT GATTCCGCCT GCCCTGGAGG ACGTGGTGGA GGAAGAGGAC 
GTGTTCCTCT CTGAAACCGG GCACAGCGGA GCAACTTCAA CACCGCACGA GTGTCCGGGG 
AACGGGGTTG AGAGACTGGC GGAGGGGAGA GAAGGAGAAG TGCGTCCTGA CAACCAGGCG 
GAGAGCAGCG AGTTCCTGCA CCTCACTATC CGGAAACAGG TGTCATACAG GAAGGCTATT 
GCCAAGTCCG GTCTCCAGCA CCTCTCATGC CAGCCCAGTG CTGCAGTGTT AGCCAAAGGG 
GAAACGGACA GAGAAATACG CAACACAGTA GACTGGAGTG AATCAGCTGT ATACGGAGAG 
CACATCTGGT TTGAGACGAA TGTCTCGGGG GACTTCTGTT ACGTTGGAGA ACAGCACTGT 
TACGCCAAGT CACTGCAAAA GTCTGTTGCT CGGAAAAAGT GTGCGGCTTG CAAGATCGTA 
GTTCACTCTA TCTGCATTGA ACAGCTGGAG AAGATAAACT TCAGATGTAA ACCGTCTTTC 
AGGGAGTCAG GATCACGGAA CATCCGAGAG CCTGCAATAG TGCGACACCA CTGGGTCCAT 
CGGAGAAGAC AAGAGGGCAA ATGCAAACAG TGTGGAAAGG GGTTTCAGCA GAAATTTGCC 
TTTCACAGTA AAGAGATTGT AGCCATCAGC TGCTCCTGGT GCAAACAAGC ATATCATAAT 
AAGGTGACAT GCTTTATGCT GCAGCAGATA GAAGAACCAT GTTCATTAGG AGCTCATGCC 
GCTGTAATAG TACCTCCTAC TTGGATCATA CGGGTTCGCC GACCTCAGTC CTCACTAAAG 
TCAAGCAAAA AGAAAAAACG GACATCTTTC AAACGTAAAT CCAGCAAGAA AGGAGCAGAG 
GAGGCACGAT GGAAGCCTTT TATAGTGAGA CCCATTCCCT CCCAACTCAT GAAGCCACTG 
CTAGTGTTTG TAAATCCAAA GAGTGGAGGA AACCAGGGAG CTAAAATCAT CCAGACCTTT 
CTTTGGTATC TAAACCCTCG GCAGGTGTTT GATCTAAGTC AAGGAGGTCC TCAGGAAGGC 
TTGGAAATGT ATCGCAAGGT TCACAACTTG AGAATTTTGG CCTGTGGGGG GGATGGCACG 
GTGGGCTGGA TCTTATCTGC ACTTGATCAG CTGCAATTAA ACCCTTCTCC TGCTGTGGCC 
GTACTTCCCC TGGGCACAGG AAATGACCTT GCTAGGACGC TAAACTGGGG TGGGGGCTAC 
ACAGATGAAC CTCTGAGTAA AATCCTGTCT CATGTGGAGG ATGGGAATAT TGTACAGCTG 
GATCGGTGGA ATCTTGTTGT GAAGCCCAAC CCAGAGGCAG GACCAGAGGA AAGAGACGAG 
CAGGTGACGG ACAAGCTCCC TCTAGACGTC TTCAATAACT ACTTCAGCCT TGGATTTGAT 
GCTCATGTGA CTTTAGAGTT CCACGAATCT CGAGAGGCTA ATCCTGAGAA ATTTAACAGC 
CGGTTCAGAA ACAAAATGTT CTATGCAGGG ACAGCGTTTT CTGATTTCTT GATGGGCAGC 
TCCAAAGACC TGGCTAAGCA CATCAAAGTA GTCTGTGACG GCACAGATTT AACCTCAAAG 
GTCCAGGATT TGAAGCTGCA ATGCCTGGTC TTCCTAAATA TCCCCAGGTA CTGTGCGGGC 
ACAATGCCAT GGGGCAATCC GAGTGAGCAT CATGACTTTG AGCCTCAGCG TCATGATGAT 
GGCTGCATTG AGGTTATTGG ATTCACAATG ACATCACTGG CCACACTGCA GGTCGGAGGT 
CACGGTGAGA GGTTAAATCA GTGTCGCGAA GTCACCCTCA CCACCTTTAA GTCAATCCCC 
ATGCAGGTGG ACGGGGAGCC TTGTAAACTA GCACCATCTG TTATTCACAT TTCTTTGCGA 
AACCAGGCCA ACATGGTGCA GAAAACCAAG AGACGTACAT CTATACCACT GCTAAACGAC 
AGTCAGCAAC CGTTTCCAGA ACGACTACGG ATCAGAGTGA GCCGTATCAG CATGCATGAT 
TATGAAGCTT TGCACTACGA CAAGGAGAAG CTCAAAGAAG CTTCGATTCC ACTGGGTCTC 
ATCGTTGTCC CTGGAGACAG TGATTTGGAA ACCTGCCGAG CTCACATCGA GAGACTACAG 
GAGGACTTTG TGTCGTGTAA CCCGTCTCTG CAGCGGCTGG TGCTCCAGGA AGGTGACGGG 
AGCAAATCAA AGGCTTTGTC GTCTCAGAGG CTATCACCGA AATGGTGCTT TCTCGACTCT 
ACAACTGCTG ATCGATTCTA CAGGATTGAT CGAGCTCAAG AGCATCTCAA CTACGTGACT 
GAAATTTCTC AGGATGAGCT GTTTGTTTTG GACCCAGAGC TGGTGGTCAC AGAGACTGTT 
AGCACATCAC CTGGCATGCC AGACCTGGTG GACTCTGGTG AAGTCCAGTC CGATCCACAC 
AAATTTGCCT TTCCGTCATC CTCTTCTTCA CCACCAAACA CACCTGGACC TAGGGTTGCA 
GAGCTGCAAA GGAAGCGTGT TTCCAGTGAC AGTTCGGTGG CAGAAGCACT TGCACACAGT 
GACTCGCTCA GATCAGCCAA GCCAGCTCTC TGCAGAGCTG GGGGTGTTCA TCGCTCAAAT 
ACAACCGCAG CAGATTTCAA ACCTACCAGC AGGTTTGAGA AGAGCGCTGT GAACAATGCC 
AACTCTGTGT CAGTGGACAG TCTAATTGAA TGTGTGAAAA AGAAGGACCA TCAGAAGTTG 
AAGGAGCTGC ATAAGAAAGG GGCCGACCTG TGTGTGCAGG ACCCTGCTGG ACGGACCCTA 
TTGCACTATG CTGTGGAAGT GGGCAGCAAG GAGATTGTCA AATACATCAT TGATAATGCA 
CCCACCGATA TCCTGGATGT AACAGAGAAA GAAAATGGAG AGACTGTGTT GCACAAAGCA 
GCCTCGCTGT GTCATAGGAC AATCTGTCAT TACCTAGTGG AAGCAGGAGC ATCTCTTATG 
AAGACAGACT TACAGGGTGA CACTCCGAAG CATCGTGCCG AGAAGGCGAA AGATGCTGAA 
CTTGCGGCAT ATCTTGAAAA CCGCCAGCAC TACCAAATGA TCCAGCGTGA GGACCAGGAG 
ACTGCAGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dgkza ( XM_001922410.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001922445.3 Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X10, mRNA.

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CloneID	ODa13284
Clone ID Related Accession (Same CDS sequence)	XM_001922410.6 , XM_001922410.5
Accession Version	XM_001922410.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	diacylglycerol kinase zeta isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001922410.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGA AATTGGACGC GACCGTTGAC GGTTCGCCAC GCGCCGCGCA CAGCTCCGCG 
GACTCGCCGA CCACCGTCCT GATTCCGCCT GCCCTGGAGG ACGTGGTGGA GGAAGAGGAC 
GTGTTCCTCT CTGAAACCGG GCACAGCGGA GCAACTTCAA CACCGCACGA GTGTCCGGGG 
AACGGGGTTG AGAGACTGGC GGAGGGGAGA GAAGGAGAAG TGCGTCCTGA CAACCAGGCG 
GAGAGCAGCG AGTTCCTGCA CCTCACTATC CGGAAACAGG TGTCATACAG GAAGGCTATT 
GCCAAGTCCG GTCTCCAGCA CCTCTCATGC CAGCCCAGTG CTGCAGTGTT AGCCAAAGGG 
GAAACGGACA GAGAAATACG CAACACAGTA GACTGGAGTG AATCAGCTGT ATACGGAGAG 
CACATCTGGT TTGAGACGAA TGTCTCGGGG GACTTCTGTT ACGTTGGAGA ACAGCACTGT 
TACGCCAAGT CACTGCAAAA GTCTGTTGCT CGGAAAAAGT GTGCGGCTTG CAAGATCGTA 
GTTCACTCTA TCTGCATTGA ACAGCTGGAG AAGATAAACT TCAGATGTAA ACCGTCTTTC 
AGGGAGTCAG GATCACGGAA CATCCGAGAG CCTGCAATAG TGCGACACCA CTGGGTCCAT 
CGGAGAAGAC AAGAGGGCAA ATGCAAACAG TGTGGAAAGG GGTTTCAGCA GAAATTTGCC 
TTTCACAGTA AAGAGATTGT AGCCATCAGC TGCTCCTGGT GCAAACAAGC ATATCATAAT 
AAGGTGACAT GCTTTATGCT GCAGCAGATA GAAGAACCAT GTTCATTAGG AGCTCATGCC 
GCTGTAATAG TACCTCCTAC TTGGATCATA CGGGTTCGCC GACCTCAGTC CTCACTAAAG 
TCAAGCAAAA AGAAAAAACG GACATCTTTC AAACGTAAAT CCAGCAAGAA AGGAGCAGAG 
GAGGCACGAT GGAAGCCTTT TATAGTGAGA CCCATTCCCT CCCAACTCAT GAAGCCACTG 
CTAGTGTTTG TAAATCCAAA GAGTGGAGGA AACCAGGGAG CTAAAATCAT CCAGACCTTT 
CTTTGGTATC TAAACCCTCG GCAGGTGTTT GATCTAAGTC AAGGAGGTCC TCAGGAAGGC 
TTGGAAATGT ATCGCAAGGT TCACAACTTG AGAATTTTGG CCTGTGGGGG GGATGGCACG 
GTGGGCTGGA TCTTATCTGC ACTTGATCAG CTGCAATTAA ACCCTTCTCC TGCTGTGGCC 
GTACTTCCCC TGGGCACAGG AAATGACCTT GCTAGGACGC TAAACTGGGG TGGGGGCTAC 
ACAGATGAAC CTCTGAGTAA AATCCTGTCT CATGTGGAGG ATGGGAATAT TGTACAGCTG 
GATCGGTGGA ATCTTGTTGT GAAGCCCAAC CCAGAGGCAG GACCAGAGGA AAGAGACGAG 
CAGGTGACGG ACAAGCTCCC TCTAGACGTC TTCAATAACT ACTTCAGCCT TGGATTTGAT 
GCTCATGTGA CTTTAGAGTT CCACGAATCT CGAGAGGCTA ATCCTGAGAA ATTTAACAGC 
CGGTTCAGAA ACAAAATGTT CTATGCAGGG ACAGCGTTTT CTGATTTCTT GATGGGCAGC 
TCCAAAGACC TGGCTAAGCA CATCAAAGTA GTCTGTGACG GCACAGATTT AACCTCAAAG 
GTCCAGGATT TGAAGCTGCA ATGCCTGGTC TTCCTAAATA TCCCCAGGTA CTGTGCGGGC 
ACAATGCCAT GGGGCAATCC GAGTGAGCAT CATGACTTTG AGCCTCAGCG TCATGATGAT 
GGCTGCATTG AGGTTATTGG ATTCACAATG ACATCACTGG CCACACTGCA GGTCGGAGGT 
CACGGTGAGA GGTTAAATCA GTGTCGCGAA GTCACCCTCA CCACCTTTAA GTCAATCCCC 
ATGCAGGTGG ACGGGGAGCC TTGTAAACTA GCACCATCTG TTATTCACAT TTCTTTGCGA 
AACCAGGCCA ACATGGTGCA GAAAACCAAG AGACGTACAT CTATACCACT GCTAAACGAC 
AGTCAGCAAC CGTTTCCAGA ACGACTACGG ATCAGAGTGA GCCGTATCAG CATGCATGAT 
TATGAAGCTT TGCACTACGA CAAGGAGAAG CTCAAAGAAG CTTCGATTCC ACTGGGTCTC 
ATCGTTGTCC CTGGAGACAG TGATTTGGAA ACCTGCCGAG CTCACATCGA GAGACTACAG 
GAGGACTTTG TGTCGTGTAA CCCGTCTCTG CAGCGGCTGG TGCTCCAGGA AGGTGACGGG 
AGCAAATCAA AGGCTTTGTC GTCTCAGAGG CTATCACCGA AATGGTGCTT TCTCGACTCT 
ACAACTGCTG ATCGATTCTA CAGGATTGAT CGAGCTCAAG AGCATCTCAA CTACGTGACT 
GAAATTTCTC AGGATGAGCT GTTTGTTTTG GACCCAGAGC TGGTGGTCAC AGAGACTGTT 
AGCACATCAC CTGGCATGCC AGACCTGGTG GACTCTGGTG AAGTCCAGTC CGATCCACAC 
AAATTTGCCT TTCCGTCATC CTCTTCTTCA CCACCAAACA CACCTGGACC TAGGGTTGCA 
GAGCTGCAAA GGAAGCGTGT TTCCAGTGAC AGTTCGGTGG CAGAAGCACT TGCACACAGT 
GACTCGCTCA GATCAGCCAA GCCAGCTCTC TGCAGAGCTG GGGGTGTTCA TCGCTCAAAT 
ACAACCGCAG CAGATTTCAA ACCTACCAGC AGGTTTGAGA AGAGCGCTGT GAACAATGCC 
AACTCTGTGT CAGTGGACAG TCTAATTGAA TGTGTGAAAA AGAAGGACCA TCAGAAGTTG 
AAGGAGCTGC ATAAGAAAGG GGCCGACCTG TGTGTGCAGG ACCCTGCTGG ACGGACCCTA 
TTGCACTATG CTGTGGAAGT GGGCAGCAAG GAGATTGTCA AATACATCAT TGATAATGCA 
CCCACCGATA TCCTGGATGT AACAGAGAAA GAAAATGGAG AGACTGTGTT GCACAAAGCA 
GCCTCGCTGT GTCATAGGAC AATCTGTCAT TACCTAGTGG AAGCAGGAGC ATCTCTTATG 
AAGACAGACT TACAGGGTGA CACTCCGAAG CATCGTGCCG AGAAGGCGAA AGATGCTGAA 
CTTGCGGCAT ATCTTGAAAA CCGCCAGCAC TACCAAATGA TCCAGCGTGA GGACCAGGAG 
ACTGCAGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001922445.3
CDS	235..3426
Translation	MAEKLDATVDGSPRAAHSSADSPTTVLIPPALEDVVEEEDVFLSETGHSGATSTPHECPGNGVERLAEGREGEVRPDNQAESSEFLHLTIRKQVSYRKAIAKSGLQHLSCQPSAAVLAKGETDREIRNTVDWSESAVYGEHIWFETNVSGDFCYVGEQHCYAKSLQKSVARKKCAACKIVVHSICIEQLEKINFRCKPSFRESGSRNIREPAIVRHHWVHRRRQEGKCKQCGKGFQQKFAFHSKEIVAISCSWCKQAYHNKVTCFMLQQIEEPCSLGAHAAVIVPPTWIIRVRRPQSSLKSSKKKKRTSFKRKSSKKGAEEARWKPFIVRPIPSQLMKPLLVFVNPKSGGNQGAKIIQTFLWYLNPRQVFDLSQGGPQEGLEMYRKVHNLRILACGGDGTVGWILSALDQLQLNPSPAVAVLPLGTGNDLARTLNWGGGYTDEPLSKILSHVEDGNIVQLDRWNLVVKPNPEAGPEERDEQVTDKLPLDVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGTAFSDFLMGSSKDLAKHIKVVCDGTDLTSKVQDLKLQCLVFLNIPRYCAGTMPWGNPSEHHDFEPQRHDDGCIEVIGFTMTSLATLQVGGHGERLNQCREVTLTTFKSIPMQVDGEPCKLAPSVIHISLRNQANMVQKTKRRTSIPLLNDSQQPFPERLRIRVSRISMHDYEALHYDKEKLKEASIPLGLIVVPGDSDLETCRAHIERLQEDFVSCNPSLQRLVLQEGDGSKSKALSSQRLSPKWCFLDSTTADRFYRIDRAQEHLNYVTEISQDELFVLDPELVVTETVSTSPGMPDLVDSGEVQSDPHKFAFPSSSSSPPNTPGPRVAELQRKRVSSDSSVAEALAHSDSLRSAKPALCRAGGVHRSNTTAADFKPTSRFEKSAVNNANSVSVDSLIECVKKKDHQKLKELHKKGADLCVQDPAGRTLLHYAVEVGSKEIVKYIIDNAPTDILDVTEKENGETVLHKAASLCHRTICHYLVEAGASLMKTDLQGDTPKHRAEKAKDAELAAYLENRQHYQMIQREDQETAV

Target ORF information:

RefSeq Version	XM_001922410.5
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001922410.5

ORF Insert Sequence:

ATGGCAGAGA AATTGGACGC GACCGTTGAC GGTTCGCCAC GCGCCGCGCA CAGCTCCGCG 
GACTCGCCGA CCACCGTCCT GATTCCGCCT GCCCTGGAGG ACGTGGTGGA GGAAGAGGAC 
GTGTTCCTCT CTGAAACCGG GCACAGCGGA GCAACTTCAA CACCGCACGA GTGTCCGGGG 
AACGGGGTTG AGAGACTGGC GGAGGGGAGA GAAGGAGAAG TGCGTCCTGA CAACCAGGCG 
GAGAGCAGCG AGTTCCTGCA CCTCACTATC CGGAAACAGG TGTCATACAG GAAGGCTATT 
GCCAAGTCCG GTCTCCAGCA CCTCTCATGC CAGCCCAGTG CTGCAGTGTT AGCCAAAGGG 
GAAACGGACA GAGAAATACG CAACACAGTA GACTGGAGTG AATCAGCTGT ATACGGAGAG 
CACATCTGGT TTGAGACGAA TGTCTCGGGG GACTTCTGTT ACGTTGGAGA ACAGCACTGT 
TACGCCAAGT CACTGCAAAA GTCTGTTGCT CGGAAAAAGT GTGCGGCTTG CAAGATCGTA 
GTTCACTCTA TCTGCATTGA ACAGCTGGAG AAGATAAACT TCAGATGTAA ACCGTCTTTC 
AGGGAGTCAG GATCACGGAA CATCCGAGAG CCTGCAATAG TGCGACACCA CTGGGTCCAT 
CGGAGAAGAC AAGAGGGCAA ATGCAAACAG TGTGGAAAGG GGTTTCAGCA GAAATTTGCC 
TTTCACAGTA AAGAGATTGT AGCCATCAGC TGCTCCTGGT GCAAACAAGC ATATCATAAT 
AAGGTGACAT GCTTTATGCT GCAGCAGATA GAAGAACCAT GTTCATTAGG AGCTCATGCC 
GCTGTAATAG TACCTCCTAC TTGGATCATA CGGGTTCGCC GACCTCAGTC CTCACTAAAG 
TCAAGCAAAA AGAAAAAACG GACATCTTTC AAACGTAAAT CCAGCAAGAA AGGAGCAGAG 
GAGGCACGAT GGAAGCCTTT TATAGTGAGA CCCATTCCCT CCCAACTCAT GAAGCCACTG 
CTAGTGTTTG TAAATCCAAA GAGTGGAGGA AACCAGGGAG CTAAAATCAT CCAGACCTTT 
CTTTGGTATC TAAACCCTCG GCAGGTGTTT GATCTAAGTC AAGGAGGTCC TCAGGAAGGC 
TTGGAAATGT ATCGCAAGGT TCACAACTTG AGAATTTTGG CCTGTGGGGG GGATGGCACG 
GTGGGCTGGA TCTTATCTGC ACTTGATCAG CTGCAATTAA ACCCTTCTCC TGCTGTGGCC 
GTACTTCCCC TGGGCACAGG AAATGACCTT GCTAGGACGC TAAACTGGGG TGGGGGCTAC 
ACAGATGAAC CTCTGAGTAA AATCCTGTCT CATGTGGAGG ATGGGAATAT TGTACAGCTG 
GATCGGTGGA ATCTTGTTGT GAAGCCCAAC CCAGAGGCAG GACCAGAGGA AAGAGACGAG 
CAGGTGACGG ACAAGCTCCC TCTAGACGTC TTCAATAACT ACTTCAGCCT TGGATTTGAT 
GCTCATGTGA CTTTAGAGTT CCACGAATCT CGAGAGGCTA ATCCTGAGAA ATTTAACAGC 
CGGTTCAGAA ACAAAATGTT CTATGCAGGG ACAGCGTTTT CTGATTTCTT GATGGGCAGC 
TCCAAAGACC TGGCTAAGCA CATCAAAGTA GTCTGTGACG GCACAGATTT AACCTCAAAG 
GTCCAGGATT TGAAGCTGCA ATGCCTGGTC TTCCTAAATA TCCCCAGGTA CTGTGCGGGC 
ACAATGCCAT GGGGCAATCC GAGTGAGCAT CATGACTTTG AGCCTCAGCG TCATGATGAT 
GGCTGCATTG AGGTTATTGG ATTCACAATG ACATCACTGG CCACACTGCA GGTCGGAGGT 
CACGGTGAGA GGTTAAATCA GTGTCGCGAA GTCACCCTCA CCACCTTTAA GTCAATCCCC 
ATGCAGGTGG ACGGGGAGCC TTGTAAACTA GCACCATCTG TTATTCACAT TTCTTTGCGA 
AACCAGGCCA ACATGGTGCA GAAAACCAAG AGACGTACAT CTATACCACT GCTAAACGAC 
AGTCAGCAAC CGTTTCCAGA ACGACTACGG ATCAGAGTGA GCCGTATCAG CATGCATGAT 
TATGAAGCTT TGCACTACGA CAAGGAGAAG CTCAAAGAAG CTTCGATTCC ACTGGGTCTC 
ATCGTTGTCC CTGGAGACAG TGATTTGGAA ACCTGCCGAG CTCACATCGA GAGACTACAG 
GAGGACTTTG TGTCGTGTAA CCCGTCTCTG CAGCGGCTGG TGCTCCAGGA AGGTGACGGG 
AGCAAATCAA AGGCTTTGTC GTCTCAGAGG CTATCACCGA AATGGTGCTT TCTCGACTCT 
ACAACTGCTG ATCGATTCTA CAGGATTGAT CGAGCTCAAG AGCATCTCAA CTACGTGACT 
GAAATTTCTC AGGATGAGCT GTTTGTTTTG GACCCAGAGC TGGTGGTCAC AGAGACTGTT 
AGCACATCAC CTGGCATGCC AGACCTGGTG GACTCTGGTG AAGTCCAGTC CGATCCACAC 
AAATTTGCCT TTCCGTCATC CTCTTCTTCA CCACCAAACA CACCTGGACC TAGGGTTGCA 
GAGCTGCAAA GGAAGCGTGT TTCCAGTGAC AGTTCGGTGG CAGAAGCACT TGCACACAGT 
GACTCGCTCA GATCAGCCAA GCCAGCTCTC TGCAGAGCTG GGGGTGTTCA TCGCTCAAAT 
ACAACCGCAG CAGATTTCAA ACCTACCAGC AGGTTTGAGA AGAGCGCTGT GAACAATGCC 
AACTCTGTGT CAGTGGACAG TCTAATTGAA TGTGTGAAAA AGAAGGACCA TCAGAAGTTG 
AAGGAGCTGC ATAAGAAAGG GGCCGACCTG TGTGTGCAGG ACCCTGCTGG ACGGACCCTA 
TTGCACTATG CTGTGGAAGT GGGCAGCAAG GAGATTGTCA AATACATCAT TGATAATGCA 
CCCACCGATA TCCTGGATGT AACAGAGAAA GAAAATGGAG AGACTGTGTT GCACAAAGCA 
GCCTCGCTGT GTCATAGGAC AATCTGTCAT TACCTAGTGG AAGCAGGAGC ATCTCTTATG 
AAGACAGACT TACAGGGTGA CACTCCGAAG CATCGTGCCG AGAAGGCGAA AGATGCTGAA 
CTTGCGGCAT ATCTTGAAAA CCGCCAGCAC TACCAAATGA TCCAGCGTGA GGACCAGGAG 
ACTGCAGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dgkza ( XM_005169036.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005169093.1 Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X11, mRNA.

Price & Availability↑

CloneID	ODa13285
Clone ID Related Accession (Same CDS sequence)	XM_005169036.3
Accession Version	XM_005169036.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3159bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	diacylglycerol kinase zeta isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169036.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAG AGCTGGGAGC AGGTTCTCAG GACAGGGAAG AGGTCACAGC CTCTCTGGCC 
TCCACGTCCG CTTCTTCATC TAACTCTGAC ATCCCATCCA AGCCTTTGCC CGAGGGGGAT 
AGAGTGGCGG TTAGTCGGCG GCACAGCAGC AGGACTTTTA CTGGGTTACG ACTGTTTGGA 
AGGAGGAAGG CTATTGCCAA GTCCGGTCTC CAGCACCTCT CATGCCAGCC CAGTGCTGCA 
GTGTTAGCCA AAGGGGAAAC GGACAGAGAA ATACGCAACA CAGTAGACTG GAGTGAATCA 
GCTGTATACG GAGAGCACAT CTGGTTTGAG ACGAATGTCT CGGGGGACTT CTGTTACGTT 
GGAGAACAGC ACTGTTACGC CAAGTCACTG CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG 
GCTTGCAAGA TCGTAGTTCA CTCTATCTGC ATTGAACAGC TGGAGAAGTT ATTGGATTGG 
AATGCTCCAC CTCAGGGGGA GGGGCTTGAT GAGATGCCTC TCAAAGAGCC GTCATTCAGA 
ATAAACTTCA GATGTAAACC GTCTTTCAGG GAGTCAGGAT CACGGAACAT CCGAGAGCCT 
GCAATAGTGC GACACCACTG GGTCCATCGG AGAAGACAAG AGGGCAAATG CAAACAGTGT 
GGAAAGGGGT TTCAGCAGAA ATTTGCCTTT CACAGTAAAG AGATTGTAGC CATCAGCTGC 
TCCTGGTGCA AACAAGCATA TCATAATAAG GTGACATGCT TTATGCTGCA GCAGATAGAA 
GAACCATGTT CATTAGGAGC TCATGCCGCT GTAATAGTAC CTCCTACTTG GATCATACGG 
GTTCGCCGAC CTCAGTCCTC ACTAAAGTCA AGCAAAAAGA AAAAACGGAC ATCTTTCAAA 
CGTAAATCCA GCAAGAAAGG AGCAGAGGAG GCACGATGGA AGCCTTTTAT AGTGAGACCC 
ATTCCCTCCC AACTCATGAA GCCACTGCTA GTGTTTGTAA ATCCAAAGAG TGGAGGAAAC 
CAGGGAGCTA AAATCATCCA GACCTTTCTT TGGTATCTAA ACCCTCGGCA GGTGTTTGAT 
CTAAGTCAAG GAGGTCCTCA GGAAGGCTTG GAAATGTATC GCAAGGTTCA CAACTTGAGA 
ATTTTGGCCT GTGGGGGGGA TGGCACGGTG GGCTGGATCT TATCTGCACT TGATCAGCTG 
CAATTAAACC CTTCTCCTGC TGTGGCCGTA CTTCCCCTGG GCACAGGAAA TGACCTTGCT 
AGGACGCTAA ACTGGGGTGG GGGCTACACA GATGAACCTC TGAGTAAAAT CCTGTCTCAT 
GTGGAGGATG GGAATATTGT ACAGCTGGAT CGGTGGAATC TTGTTGTGAA GCCCAACCCA 
GAGGCAGGAC CAGAGGAAAG AGACGAGCAG GTGACGGACA AGCTCCCTCT AGACGTCTTC 
AATAACTACT TCAGCCTTGG ATTTGATGCT CATGTGACTT TAGAGTTCCA CGAATCTCGA 
GAGGCTAATC CTGAGAAATT TAACAGCCGG TTCAGAAACA AAATGTTCTA TGCAGGGACA 
GCGTTTTCTG ATTTCTTGAT GGGCAGCTCC AAAGACCTGG CTAAGCACAT CAAAGTAGTC 
TGTGACGGCA CAGATTTAAC CTCAAAGGTC CAGGATTTGA AGCTGCAATG CCTGGTCTTC 
CTAAATATCC CCAGGTACTG TGCGGGCACA ATGCCATGGG GCAATCCGAG TGAGCATCAT 
GACTTTGAGC CTCAGCGTCA TGATGATGGC TGCATTGAGG TTATTGGATT CACAATGACA 
TCACTGGCCA CACTGCAGGT CGGAGGTCAC GGTGAGAGGT TAAATCAGTG TCGCGAAGTC 
ACCCTCACCA CCTTTAAGTC AATCCCCATG CAGGTGGACG GGGAGCCTTG TAAACTAGCA 
CCATCTGTTA TTCACATTTC TTTGCGAAAC CAGGCCAACA TGGTGCAGAA AACCAAGAGA 
CGTACATCTA TACCACTGCT AAACGACAGT CAGCAACCGT TTCCAGAACG ACTACGGATC 
AGAGTGAGCC GTATCAGCAT GCATGATTAT GAAGCTTTGC ACTACGACAA GGAGAAGCTC 
AAAGAAGCTT CGATTCCACT GGGTCTCATC GTTGTCCCTG GAGACAGTGA TTTGGAAACC 
TGCCGAGCTC ACATCGAGAG ACTACAGGAG GACTTTGTGT CGTGTAACCC GTCTCTGCAG 
CGGCTGGTGC TCCAGGAAGG TGACGGGAGC AAATCAAAGG CTTTGTCGTC TCAGAGGCTA 
TCACCGAAAT GGTGCTTTCT CGACTCTACA ACTGCTGATC GATTCTACAG GATTGATCGA 
GCTCAAGAGC ATCTCAACTA CGTGACTGAA ATTTCTCAGG ATGAGCTGTT TGTTTTGGAC 
CCAGAGCTGG TGGTCACAGA GACTGTTAGC ACATCACCTG GCATGCCAGA CCTGGTGGAC 
TCTGGTGAAG TCCAGTCCGA TCCACACAAA TTTGCCTTTC CGTCATCCTC TTCTTCACCA 
CCAAACACAC CTGGACCTAG GGTTGCAGAG CTGCAAAGGA AGCGTGTTTC CAGTGACAGT 
TCGGTGGCAG AAGCACTTGC ACACAGTGAC TCGCTCAGAT CAGCCAAGCC AGCTCTCTGC 
AGAGCTGGGG GTGTTCATCG CTCAAATACA ACCGCAGCAG ATTTCAAACC TACCAGCAGG 
TTTGAGAAGA GCGCTGTGAA CAATGCCAAC TCTGTGTCAG TGGACAGTCT AATTGAATGT 
GTGAAAAAGA AGGACCATCA GAAGTTGAAG GAGCTGCATA AGAAAGGGGC CGACCTGTGT 
GTGCAGGACC CTGCTGGACG GACCCTATTG CACTATGCTG TGGAAGTGGG CAGCAAGGAG 
ATTGTCAAAT ACATCATTGA TAATGCACCC ACCGATATCC TGGATGTAAC AGAGAAAGAA 
AATGGAGAGA CTGTGTTGCA CAAAGCAGCC TCGCTGTGTC ATAGGACAAT CTGTCATTAC 
CTAGTGGAAG CAGGAGCATC TCTTATGAAG ACAGACTTAC AGGGTGACAC TCCGAAGCAT 
CGTGCCGAGA AGGCGAAAGA TGCTGAACTT GCGGCATATC TTGAAAACCG CCAGCACTAC 
CAAATGATCC AGCGTGAGGA CCAGGAGACT GCAGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005169093.1
CDS	513..3671
Translation	MEQELGAGSQDREEVTASLASTSASSSNSDIPSKPLPEGDRVAVSRRHSSRTFTGLRLFGRRKAIAKSGLQHLSCQPSAAVLAKGETDREIRNTVDWSESAVYGEHIWFETNVSGDFCYVGEQHCYAKSLQKSVARKKCAACKIVVHSICIEQLEKLLDWNAPPQGEGLDEMPLKEPSFRINFRCKPSFRESGSRNIREPAIVRHHWVHRRRQEGKCKQCGKGFQQKFAFHSKEIVAISCSWCKQAYHNKVTCFMLQQIEEPCSLGAHAAVIVPPTWIIRVRRPQSSLKSSKKKKRTSFKRKSSKKGAEEARWKPFIVRPIPSQLMKPLLVFVNPKSGGNQGAKIIQTFLWYLNPRQVFDLSQGGPQEGLEMYRKVHNLRILACGGDGTVGWILSALDQLQLNPSPAVAVLPLGTGNDLARTLNWGGGYTDEPLSKILSHVEDGNIVQLDRWNLVVKPNPEAGPEERDEQVTDKLPLDVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGTAFSDFLMGSSKDLAKHIKVVCDGTDLTSKVQDLKLQCLVFLNIPRYCAGTMPWGNPSEHHDFEPQRHDDGCIEVIGFTMTSLATLQVGGHGERLNQCREVTLTTFKSIPMQVDGEPCKLAPSVIHISLRNQANMVQKTKRRTSIPLLNDSQQPFPERLRIRVSRISMHDYEALHYDKEKLKEASIPLGLIVVPGDSDLETCRAHIERLQEDFVSCNPSLQRLVLQEGDGSKSKALSSQRLSPKWCFLDSTTADRFYRIDRAQEHLNYVTEISQDELFVLDPELVVTETVSTSPGMPDLVDSGEVQSDPHKFAFPSSSSSPPNTPGPRVAELQRKRVSSDSSVAEALAHSDSLRSAKPALCRAGGVHRSNTTAADFKPTSRFEKSAVNNANSVSVDSLIECVKKKDHQKLKELHKKGADLCVQDPAGRTLLHYAVEVGSKEIVKYIIDNAPTDILDVTEKENGETVLHKAASLCHRTICHYLVEAGASLMKTDLQGDTPKHRAEKAKDAELAAYLENRQHYQMIQREDQETAV

Target ORF information:

RefSeq Version	XM_005169036.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005169036.3

ORF Insert Sequence:

ATGGAGCAAG AGCTGGGAGC AGGTTCTCAG GACAGGGAAG AGGTCACAGC CTCTCTGGCC 
TCCACGTCCG CTTCTTCATC TAACTCTGAC ATCCCATCCA AGCCTTTGCC CGAGGGGGAT 
AGAGTGGCGG TTAGTCGGCG GCACAGCAGC AGGACTTTTA CTGGGTTACG ACTGTTTGGA 
AGGAGGAAGG CTATTGCCAA GTCCGGTCTC CAGCACCTCT CATGCCAGCC CAGTGCTGCA 
GTGTTAGCCA AAGGGGAAAC GGACAGAGAA ATACGCAACA CAGTAGACTG GAGTGAATCA 
GCTGTATACG GAGAGCACAT CTGGTTTGAG ACGAATGTCT CGGGGGACTT CTGTTACGTT 
GGAGAACAGC ACTGTTACGC CAAGTCACTG CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG 
GCTTGCAAGA TCGTAGTTCA CTCTATCTGC ATTGAACAGC TGGAGAAGTT ATTGGATTGG 
AATGCTCCAC CTCAGGGGGA GGGGCTTGAT GAGATGCCTC TCAAAGAGCC GTCATTCAGA 
ATAAACTTCA GATGTAAACC GTCTTTCAGG GAGTCAGGAT CACGGAACAT CCGAGAGCCT 
GCAATAGTGC GACACCACTG GGTCCATCGG AGAAGACAAG AGGGCAAATG CAAACAGTGT 
GGAAAGGGGT TTCAGCAGAA ATTTGCCTTT CACAGTAAAG AGATTGTAGC CATCAGCTGC 
TCCTGGTGCA AACAAGCATA TCATAATAAG GTGACATGCT TTATGCTGCA GCAGATAGAA 
GAACCATGTT CATTAGGAGC TCATGCCGCT GTAATAGTAC CTCCTACTTG GATCATACGG 
GTTCGCCGAC CTCAGTCCTC ACTAAAGTCA AGCAAAAAGA AAAAACGGAC ATCTTTCAAA 
CGTAAATCCA GCAAGAAAGG AGCAGAGGAG GCACGATGGA AGCCTTTTAT AGTGAGACCC 
ATTCCCTCCC AACTCATGAA GCCACTGCTA GTGTTTGTAA ATCCAAAGAG TGGAGGAAAC 
CAGGGAGCTA AAATCATCCA GACCTTTCTT TGGTATCTAA ACCCTCGGCA GGTGTTTGAT 
CTAAGTCAAG GAGGTCCTCA GGAAGGCTTG GAAATGTATC GCAAGGTTCA CAACTTGAGA 
ATTTTGGCCT GTGGGGGGGA TGGCACGGTG GGCTGGATCT TATCTGCACT TGATCAGCTG 
CAATTAAACC CTTCTCCTGC TGTGGCCGTA CTTCCCCTGG GCACAGGAAA TGACCTTGCT 
AGGACGCTAA ACTGGGGTGG GGGCTACACA GATGAACCTC TGAGTAAAAT CCTGTCTCAT 
GTGGAGGATG GGAATATTGT ACAGCTGGAT CGGTGGAATC TTGTTGTGAA GCCCAACCCA 
GAGGCAGGAC CAGAGGAAAG AGACGAGCAG GTGACGGACA AGCTCCCTCT AGACGTCTTC 
AATAACTACT TCAGCCTTGG ATTTGATGCT CATGTGACTT TAGAGTTCCA CGAATCTCGA 
GAGGCTAATC CTGAGAAATT TAACAGCCGG TTCAGAAACA AAATGTTCTA TGCAGGGACA 
GCGTTTTCTG ATTTCTTGAT GGGCAGCTCC AAAGACCTGG CTAAGCACAT CAAAGTAGTC 
TGTGACGGCA CAGATTTAAC CTCAAAGGTC CAGGATTTGA AGCTGCAATG CCTGGTCTTC 
CTAAATATCC CCAGGTACTG TGCGGGCACA ATGCCATGGG GCAATCCGAG TGAGCATCAT 
GACTTTGAGC CTCAGCGTCA TGATGATGGC TGCATTGAGG TTATTGGATT CACAATGACA 
TCACTGGCCA CACTGCAGGT CGGAGGTCAC GGTGAGAGGT TAAATCAGTG TCGCGAAGTC 
ACCCTCACCA CCTTTAAGTC AATCCCCATG CAGGTGGACG GGGAGCCTTG TAAACTAGCA 
CCATCTGTTA TTCACATTTC TTTGCGAAAC CAGGCCAACA TGGTGCAGAA AACCAAGAGA 
CGTACATCTA TACCACTGCT AAACGACAGT CAGCAACCGT TTCCAGAACG ACTACGGATC 
AGAGTGAGCC GTATCAGCAT GCATGATTAT GAAGCTTTGC ACTACGACAA GGAGAAGCTC 
AAAGAAGCTT CGATTCCACT GGGTCTCATC GTTGTCCCTG GAGACAGTGA TTTGGAAACC 
TGCCGAGCTC ACATCGAGAG ACTACAGGAG GACTTTGTGT CGTGTAACCC GTCTCTGCAG 
CGGCTGGTGC TCCAGGAAGG TGACGGGAGC AAATCAAAGG CTTTGTCGTC TCAGAGGCTA 
TCACCGAAAT GGTGCTTTCT CGACTCTACA ACTGCTGATC GATTCTACAG GATTGATCGA 
GCTCAAGAGC ATCTCAACTA CGTGACTGAA ATTTCTCAGG ATGAGCTGTT TGTTTTGGAC 
CCAGAGCTGG TGGTCACAGA GACTGTTAGC ACATCACCTG GCATGCCAGA CCTGGTGGAC 
TCTGGTGAAG TCCAGTCCGA TCCACACAAA TTTGCCTTTC CGTCATCCTC TTCTTCACCA 
CCAAACACAC CTGGACCTAG GGTTGCAGAG CTGCAAAGGA AGCGTGTTTC CAGTGACAGT 
TCGGTGGCAG AAGCACTTGC ACACAGTGAC TCGCTCAGAT CAGCCAAGCC AGCTCTCTGC 
AGAGCTGGGG GTGTTCATCG CTCAAATACA ACCGCAGCAG ATTTCAAACC TACCAGCAGG 
TTTGAGAAGA GCGCTGTGAA CAATGCCAAC TCTGTGTCAG TGGACAGTCT AATTGAATGT 
GTGAAAAAGA AGGACCATCA GAAGTTGAAG GAGCTGCATA AGAAAGGGGC CGACCTGTGT 
GTGCAGGACC CTGCTGGACG GACCCTATTG CACTATGCTG TGGAAGTGGG CAGCAAGGAG 
ATTGTCAAAT ACATCATTGA TAATGCACCC ACCGATATCC TGGATGTAAC AGAGAAAGAA 
AATGGAGAGA CTGTGTTGCA CAAAGCAGCC TCGCTGTGTC ATAGGACAAT CTGTCATTAC 
CTAGTGGAAG CAGGAGCATC TCTTATGAAG ACAGACTTAC AGGGTGACAC TCCGAAGCAT 
CGTGCCGAGA AGGCGAAAGA TGCTGAACTT GCGGCATATC TTGAAAACCG CCAGCACTAC 
CAAATGATCC AGCGTGAGGA CCAGGAGACT GCAGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dgkza ( XM_005169037.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005169094.1 Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X12, mRNA.

Price & Availability↑

CloneID	ODa13286
Clone ID Related Accession (Same CDS sequence)	XM_005169037.3 , XM_005169037.4
Accession Version	XM_005169037.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3087bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	diacylglycerol kinase zeta isoform X12
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169037.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAG AGCTGGGAGC AGGTTCTCAG GACAGGGAAG AGGTCACAGC CTCTCTGGCC 
TCCACGTCCG CTTCTTCATC TAACTCTGAC ATCCCATCCA AGCCTTTGCC CGAGGGGGAT 
AGAGTGGCGG TTAGTCGGCG GCACAGCAGC AGGACTTTTA CTGGGTTACG ACTGTTTGGA 
AGGAGGAAGG CTATTGCCAA GTCCGGTCTC CAGCACCTCT CATGCCAGCC CAGTGCTGCA 
GTGTTAGCCA AAGGGGAAAC GGACAGAGAA ATACGCAACA CAGTAGACTG GAGTGAATCA 
GCTGTATACG GAGAGCACAT CTGGTTTGAG ACGAATGTCT CGGGGGACTT CTGTTACGTT 
GGAGAACAGC ACTGTTACGC CAAGTCACTG CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG 
GCTTGCAAGA TCGTAGTTCA CTCTATCTGC ATTGAACAGC TGGAGAAGAT AAACTTCAGA 
TGTAAACCGT CTTTCAGGGA GTCAGGATCA CGGAACATCC GAGAGCCTGC AATAGTGCGA 
CACCACTGGG TCCATCGGAG AAGACAAGAG GGCAAATGCA AACAGTGTGG AAAGGGGTTT 
CAGCAGAAAT TTGCCTTTCA CAGTAAAGAG ATTGTAGCCA TCAGCTGCTC CTGGTGCAAA 
CAAGCATATC ATAATAAGGT GACATGCTTT ATGCTGCAGC AGATAGAAGA ACCATGTTCA 
TTAGGAGCTC ATGCCGCTGT AATAGTACCT CCTACTTGGA TCATACGGGT TCGCCGACCT 
CAGTCCTCAC TAAAGTCAAG CAAAAAGAAA AAACGGACAT CTTTCAAACG TAAATCCAGC 
AAGAAAGGAG CAGAGGAGGC ACGATGGAAG CCTTTTATAG TGAGACCCAT TCCCTCCCAA 
CTCATGAAGC CACTGCTAGT GTTTGTAAAT CCAAAGAGTG GAGGAAACCA GGGAGCTAAA 
ATCATCCAGA CCTTTCTTTG GTATCTAAAC CCTCGGCAGG TGTTTGATCT AAGTCAAGGA 
GGTCCTCAGG AAGGCTTGGA AATGTATCGC AAGGTTCACA ACTTGAGAAT TTTGGCCTGT 
GGGGGGGATG GCACGGTGGG CTGGATCTTA TCTGCACTTG ATCAGCTGCA ATTAAACCCT 
TCTCCTGCTG TGGCCGTACT TCCCCTGGGC ACAGGAAATG ACCTTGCTAG GACGCTAAAC 
TGGGGTGGGG GCTACACAGA TGAACCTCTG AGTAAAATCC TGTCTCATGT GGAGGATGGG 
AATATTGTAC AGCTGGATCG GTGGAATCTT GTTGTGAAGC CCAACCCAGA GGCAGGACCA 
GAGGAAAGAG ACGAGCAGGT GACGGACAAG CTCCCTCTAG ACGTCTTCAA TAACTACTTC 
AGCCTTGGAT TTGATGCTCA TGTGACTTTA GAGTTCCACG AATCTCGAGA GGCTAATCCT 
GAGAAATTTA ACAGCCGGTT CAGAAACAAA ATGTTCTATG CAGGGACAGC GTTTTCTGAT 
TTCTTGATGG GCAGCTCCAA AGACCTGGCT AAGCACATCA AAGTAGTCTG TGACGGCACA 
GATTTAACCT CAAAGGTCCA GGATTTGAAG CTGCAATGCC TGGTCTTCCT AAATATCCCC 
AGGTACTGTG CGGGCACAAT GCCATGGGGC AATCCGAGTG AGCATCATGA CTTTGAGCCT 
CAGCGTCATG ATGATGGCTG CATTGAGGTT ATTGGATTCA CAATGACATC ACTGGCCACA 
CTGCAGGTCG GAGGTCACGG TGAGAGGTTA AATCAGTGTC GCGAAGTCAC CCTCACCACC 
TTTAAGTCAA TCCCCATGCA GGTGGACGGG GAGCCTTGTA AACTAGCACC ATCTGTTATT 
CACATTTCTT TGCGAAACCA GGCCAACATG GTGCAGAAAA CCAAGAGACG TACATCTATA 
CCACTGCTAA ACGACAGTCA GCAACCGTTT CCAGAACGAC TACGGATCAG AGTGAGCCGT 
ATCAGCATGC ATGATTATGA AGCTTTGCAC TACGACAAGG AGAAGCTCAA AGAAGCTTCG 
ATTCCACTGG GTCTCATCGT TGTCCCTGGA GACAGTGATT TGGAAACCTG CCGAGCTCAC 
ATCGAGAGAC TACAGGAGGA CTTTGTGTCG TGTAACCCGT CTCTGCAGCG GCTGGTGCTC 
CAGGAAGGTG ACGGGAGCAA ATCAAAGGCT TTGTCGTCTC AGAGGCTATC ACCGAAATGG 
TGCTTTCTCG ACTCTACAAC TGCTGATCGA TTCTACAGGA TTGATCGAGC TCAAGAGCAT 
CTCAACTACG TGACTGAAAT TTCTCAGGAT GAGCTGTTTG TTTTGGACCC AGAGCTGGTG 
GTCACAGAGA CTGTTAGCAC ATCACCTGGC ATGCCAGACC TGGTGGACTC TGGTGAAGTC 
CAGTCCGATC CACACAAATT TGCCTTTCCG TCATCCTCTT CTTCACCACC AAACACACCT 
GGACCTAGGG TTGCAGAGCT GCAAAGGAAG CGTGTTTCCA GTGACAGTTC GGTGGCAGAA 
GCACTTGCAC ACAGTGACTC GCTCAGATCA GCCAAGCCAG CTCTCTGCAG AGCTGGGGGT 
GTTCATCGCT CAAATACAAC CGCAGCAGAT TTCAAACCTA CCAGCAGGTT TGAGAAGAGC 
GCTGTGAACA ATGCCAACTC TGTGTCAGTG GACAGTCTAA TTGAATGTGT GAAAAAGAAG 
GACCATCAGA AGTTGAAGGA GCTGCATAAG AAAGGGGCCG ACCTGTGTGT GCAGGACCCT 
GCTGGACGGA CCCTATTGCA CTATGCTGTG GAAGTGGGCA GCAAGGAGAT TGTCAAATAC 
ATCATTGATA ATGCACCCAC CGATATCCTG GATGTAACAG AGAAAGAAAA TGGAGAGACT 
GTGTTGCACA AAGCAGCCTC GCTGTGTCAT AGGACAATCT GTCATTACCT AGTGGAAGCA 
GGAGCATCTC TTATGAAGAC AGACTTACAG GGTGACACTC CGAAGCATCG TGCCGAGAAG 
GCGAAAGATG CTGAACTTGC GGCATATCTT GAAAACCGCC AGCACTACCA AATGATCCAG 
CGTGAGGACC AGGAGACTGC AGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005169094.1
CDS	513..3599
Translation	MEQELGAGSQDREEVTASLASTSASSSNSDIPSKPLPEGDRVAVSRRHSSRTFTGLRLFGRRKAIAKSGLQHLSCQPSAAVLAKGETDREIRNTVDWSESAVYGEHIWFETNVSGDFCYVGEQHCYAKSLQKSVARKKCAACKIVVHSICIEQLEKINFRCKPSFRESGSRNIREPAIVRHHWVHRRRQEGKCKQCGKGFQQKFAFHSKEIVAISCSWCKQAYHNKVTCFMLQQIEEPCSLGAHAAVIVPPTWIIRVRRPQSSLKSSKKKKRTSFKRKSSKKGAEEARWKPFIVRPIPSQLMKPLLVFVNPKSGGNQGAKIIQTFLWYLNPRQVFDLSQGGPQEGLEMYRKVHNLRILACGGDGTVGWILSALDQLQLNPSPAVAVLPLGTGNDLARTLNWGGGYTDEPLSKILSHVEDGNIVQLDRWNLVVKPNPEAGPEERDEQVTDKLPLDVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGTAFSDFLMGSSKDLAKHIKVVCDGTDLTSKVQDLKLQCLVFLNIPRYCAGTMPWGNPSEHHDFEPQRHDDGCIEVIGFTMTSLATLQVGGHGERLNQCREVTLTTFKSIPMQVDGEPCKLAPSVIHISLRNQANMVQKTKRRTSIPLLNDSQQPFPERLRIRVSRISMHDYEALHYDKEKLKEASIPLGLIVVPGDSDLETCRAHIERLQEDFVSCNPSLQRLVLQEGDGSKSKALSSQRLSPKWCFLDSTTADRFYRIDRAQEHLNYVTEISQDELFVLDPELVVTETVSTSPGMPDLVDSGEVQSDPHKFAFPSSSSSPPNTPGPRVAELQRKRVSSDSSVAEALAHSDSLRSAKPALCRAGGVHRSNTTAADFKPTSRFEKSAVNNANSVSVDSLIECVKKKDHQKLKELHKKGADLCVQDPAGRTLLHYAVEVGSKEIVKYIIDNAPTDILDVTEKENGETVLHKAASLCHRTICHYLVEAGASLMKTDLQGDTPKHRAEKAKDAELAAYLENRQHYQMIQREDQETAV

Target ORF information:

RefSeq Version	XM_005169037.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005169037.3

ORF Insert Sequence:

ATGGAGCAAG AGCTGGGAGC AGGTTCTCAG GACAGGGAAG AGGTCACAGC CTCTCTGGCC 
TCCACGTCCG CTTCTTCATC TAACTCTGAC ATCCCATCCA AGCCTTTGCC CGAGGGGGAT 
AGAGTGGCGG TTAGTCGGCG GCACAGCAGC AGGACTTTTA CTGGGTTACG ACTGTTTGGA 
AGGAGGAAGG CTATTGCCAA GTCCGGTCTC CAGCACCTCT CATGCCAGCC CAGTGCTGCA 
GTGTTAGCCA AAGGGGAAAC GGACAGAGAA ATACGCAACA CAGTAGACTG GAGTGAATCA 
GCTGTATACG GAGAGCACAT CTGGTTTGAG ACGAATGTCT CGGGGGACTT CTGTTACGTT 
GGAGAACAGC ACTGTTACGC CAAGTCACTG CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG 
GCTTGCAAGA TCGTAGTTCA CTCTATCTGC ATTGAACAGC TGGAGAAGAT AAACTTCAGA 
TGTAAACCGT CTTTCAGGGA GTCAGGATCA CGGAACATCC GAGAGCCTGC AATAGTGCGA 
CACCACTGGG TCCATCGGAG AAGACAAGAG GGCAAATGCA AACAGTGTGG AAAGGGGTTT 
CAGCAGAAAT TTGCCTTTCA CAGTAAAGAG ATTGTAGCCA TCAGCTGCTC CTGGTGCAAA 
CAAGCATATC ATAATAAGGT GACATGCTTT ATGCTGCAGC AGATAGAAGA ACCATGTTCA 
TTAGGAGCTC ATGCCGCTGT AATAGTACCT CCTACTTGGA TCATACGGGT TCGCCGACCT 
CAGTCCTCAC TAAAGTCAAG CAAAAAGAAA AAACGGACAT CTTTCAAACG TAAATCCAGC 
AAGAAAGGAG CAGAGGAGGC ACGATGGAAG CCTTTTATAG TGAGACCCAT TCCCTCCCAA 
CTCATGAAGC CACTGCTAGT GTTTGTAAAT CCAAAGAGTG GAGGAAACCA GGGAGCTAAA 
ATCATCCAGA CCTTTCTTTG GTATCTAAAC CCTCGGCAGG TGTTTGATCT AAGTCAAGGA 
GGTCCTCAGG AAGGCTTGGA AATGTATCGC AAGGTTCACA ACTTGAGAAT TTTGGCCTGT 
GGGGGGGATG GCACGGTGGG CTGGATCTTA TCTGCACTTG ATCAGCTGCA ATTAAACCCT 
TCTCCTGCTG TGGCCGTACT TCCCCTGGGC ACAGGAAATG ACCTTGCTAG GACGCTAAAC 
TGGGGTGGGG GCTACACAGA TGAACCTCTG AGTAAAATCC TGTCTCATGT GGAGGATGGG 
AATATTGTAC AGCTGGATCG GTGGAATCTT GTTGTGAAGC CCAACCCAGA GGCAGGACCA 
GAGGAAAGAG ACGAGCAGGT GACGGACAAG CTCCCTCTAG ACGTCTTCAA TAACTACTTC 
AGCCTTGGAT TTGATGCTCA TGTGACTTTA GAGTTCCACG AATCTCGAGA GGCTAATCCT 
GAGAAATTTA ACAGCCGGTT CAGAAACAAA ATGTTCTATG CAGGGACAGC GTTTTCTGAT 
TTCTTGATGG GCAGCTCCAA AGACCTGGCT AAGCACATCA AAGTAGTCTG TGACGGCACA 
GATTTAACCT CAAAGGTCCA GGATTTGAAG CTGCAATGCC TGGTCTTCCT AAATATCCCC 
AGGTACTGTG CGGGCACAAT GCCATGGGGC AATCCGAGTG AGCATCATGA CTTTGAGCCT 
CAGCGTCATG ATGATGGCTG CATTGAGGTT ATTGGATTCA CAATGACATC ACTGGCCACA 
CTGCAGGTCG GAGGTCACGG TGAGAGGTTA AATCAGTGTC GCGAAGTCAC CCTCACCACC 
TTTAAGTCAA TCCCCATGCA GGTGGACGGG GAGCCTTGTA AACTAGCACC ATCTGTTATT 
CACATTTCTT TGCGAAACCA GGCCAACATG GTGCAGAAAA CCAAGAGACG TACATCTATA 
CCACTGCTAA ACGACAGTCA GCAACCGTTT CCAGAACGAC TACGGATCAG AGTGAGCCGT 
ATCAGCATGC ATGATTATGA AGCTTTGCAC TACGACAAGG AGAAGCTCAA AGAAGCTTCG 
ATTCCACTGG GTCTCATCGT TGTCCCTGGA GACAGTGATT TGGAAACCTG CCGAGCTCAC 
ATCGAGAGAC TACAGGAGGA CTTTGTGTCG TGTAACCCGT CTCTGCAGCG GCTGGTGCTC 
CAGGAAGGTG ACGGGAGCAA ATCAAAGGCT TTGTCGTCTC AGAGGCTATC ACCGAAATGG 
TGCTTTCTCG ACTCTACAAC TGCTGATCGA TTCTACAGGA TTGATCGAGC TCAAGAGCAT 
CTCAACTACG TGACTGAAAT TTCTCAGGAT GAGCTGTTTG TTTTGGACCC AGAGCTGGTG 
GTCACAGAGA CTGTTAGCAC ATCACCTGGC ATGCCAGACC TGGTGGACTC TGGTGAAGTC 
CAGTCCGATC CACACAAATT TGCCTTTCCG TCATCCTCTT CTTCACCACC AAACACACCT 
GGACCTAGGG TTGCAGAGCT GCAAAGGAAG CGTGTTTCCA GTGACAGTTC GGTGGCAGAA 
GCACTTGCAC ACAGTGACTC GCTCAGATCA GCCAAGCCAG CTCTCTGCAG AGCTGGGGGT 
GTTCATCGCT CAAATACAAC CGCAGCAGAT TTCAAACCTA CCAGCAGGTT TGAGAAGAGC 
GCTGTGAACA ATGCCAACTC TGTGTCAGTG GACAGTCTAA TTGAATGTGT GAAAAAGAAG 
GACCATCAGA AGTTGAAGGA GCTGCATAAG AAAGGGGCCG ACCTGTGTGT GCAGGACCCT 
GCTGGACGGA CCCTATTGCA CTATGCTGTG GAAGTGGGCA GCAAGGAGAT TGTCAAATAC 
ATCATTGATA ATGCACCCAC CGATATCCTG GATGTAACAG AGAAAGAAAA TGGAGAGACT 
GTGTTGCACA AAGCAGCCTC GCTGTGTCAT AGGACAATCT GTCATTACCT AGTGGAAGCA 
GGAGCATCTC TTATGAAGAC AGACTTACAG GGTGACACTC CGAAGCATCG TGCCGAGAAG 
GCGAAAGATG CTGAACTTGC GGCATATCTT GAAAACCGCC AGCACTACCA AATGATCCAG 
CGTGAGGACC AGGAGACTGC AGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dgkza ( XM_005169037.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005169094.1 Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ODa13286
Clone ID Related Accession (Same CDS sequence)	XM_005169037.3 , XM_005169037.4
Accession Version	XM_005169037.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3087bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	diacylglycerol kinase zeta isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005169037.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAG AGCTGGGAGC AGGTTCTCAG GACAGGGAAG AGGTCACAGC CTCTCTGGCC 
TCCACGTCCG CTTCTTCATC TAACTCTGAC ATCCCATCCA AGCCTTTGCC CGAGGGGGAT 
AGAGTGGCGG TTAGTCGGCG GCACAGCAGC AGGACTTTTA CTGGGTTACG ACTGTTTGGA 
AGGAGGAAGG CTATTGCCAA GTCCGGTCTC CAGCACCTCT CATGCCAGCC CAGTGCTGCA 
GTGTTAGCCA AAGGGGAAAC GGACAGAGAA ATACGCAACA CAGTAGACTG GAGTGAATCA 
GCTGTATACG GAGAGCACAT CTGGTTTGAG ACGAATGTCT CGGGGGACTT CTGTTACGTT 
GGAGAACAGC ACTGTTACGC CAAGTCACTG CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG 
GCTTGCAAGA TCGTAGTTCA CTCTATCTGC ATTGAACAGC TGGAGAAGAT AAACTTCAGA 
TGTAAACCGT CTTTCAGGGA GTCAGGATCA CGGAACATCC GAGAGCCTGC AATAGTGCGA 
CACCACTGGG TCCATCGGAG AAGACAAGAG GGCAAATGCA AACAGTGTGG AAAGGGGTTT 
CAGCAGAAAT TTGCCTTTCA CAGTAAAGAG ATTGTAGCCA TCAGCTGCTC CTGGTGCAAA 
CAAGCATATC ATAATAAGGT GACATGCTTT ATGCTGCAGC AGATAGAAGA ACCATGTTCA 
TTAGGAGCTC ATGCCGCTGT AATAGTACCT CCTACTTGGA TCATACGGGT TCGCCGACCT 
CAGTCCTCAC TAAAGTCAAG CAAAAAGAAA AAACGGACAT CTTTCAAACG TAAATCCAGC 
AAGAAAGGAG CAGAGGAGGC ACGATGGAAG CCTTTTATAG TGAGACCCAT TCCCTCCCAA 
CTCATGAAGC CACTGCTAGT GTTTGTAAAT CCAAAGAGTG GAGGAAACCA GGGAGCTAAA 
ATCATCCAGA CCTTTCTTTG GTATCTAAAC CCTCGGCAGG TGTTTGATCT AAGTCAAGGA 
GGTCCTCAGG AAGGCTTGGA AATGTATCGC AAGGTTCACA ACTTGAGAAT TTTGGCCTGT 
GGGGGGGATG GCACGGTGGG CTGGATCTTA TCTGCACTTG ATCAGCTGCA ATTAAACCCT 
TCTCCTGCTG TGGCCGTACT TCCCCTGGGC ACAGGAAATG ACCTTGCTAG GACGCTAAAC 
TGGGGTGGGG GCTACACAGA TGAACCTCTG AGTAAAATCC TGTCTCATGT GGAGGATGGG 
AATATTGTAC AGCTGGATCG GTGGAATCTT GTTGTGAAGC CCAACCCAGA GGCAGGACCA 
GAGGAAAGAG ACGAGCAGGT GACGGACAAG CTCCCTCTAG ACGTCTTCAA TAACTACTTC 
AGCCTTGGAT TTGATGCTCA TGTGACTTTA GAGTTCCACG AATCTCGAGA GGCTAATCCT 
GAGAAATTTA ACAGCCGGTT CAGAAACAAA ATGTTCTATG CAGGGACAGC GTTTTCTGAT 
TTCTTGATGG GCAGCTCCAA AGACCTGGCT AAGCACATCA AAGTAGTCTG TGACGGCACA 
GATTTAACCT CAAAGGTCCA GGATTTGAAG CTGCAATGCC TGGTCTTCCT AAATATCCCC 
AGGTACTGTG CGGGCACAAT GCCATGGGGC AATCCGAGTG AGCATCATGA CTTTGAGCCT 
CAGCGTCATG ATGATGGCTG CATTGAGGTT ATTGGATTCA CAATGACATC ACTGGCCACA 
CTGCAGGTCG GAGGTCACGG TGAGAGGTTA AATCAGTGTC GCGAAGTCAC CCTCACCACC 
TTTAAGTCAA TCCCCATGCA GGTGGACGGG GAGCCTTGTA AACTAGCACC ATCTGTTATT 
CACATTTCTT TGCGAAACCA GGCCAACATG GTGCAGAAAA CCAAGAGACG TACATCTATA 
CCACTGCTAA ACGACAGTCA GCAACCGTTT CCAGAACGAC TACGGATCAG AGTGAGCCGT 
ATCAGCATGC ATGATTATGA AGCTTTGCAC TACGACAAGG AGAAGCTCAA AGAAGCTTCG 
ATTCCACTGG GTCTCATCGT TGTCCCTGGA GACAGTGATT TGGAAACCTG CCGAGCTCAC 
ATCGAGAGAC TACAGGAGGA CTTTGTGTCG TGTAACCCGT CTCTGCAGCG GCTGGTGCTC 
CAGGAAGGTG ACGGGAGCAA ATCAAAGGCT TTGTCGTCTC AGAGGCTATC ACCGAAATGG 
TGCTTTCTCG ACTCTACAAC TGCTGATCGA TTCTACAGGA TTGATCGAGC TCAAGAGCAT 
CTCAACTACG TGACTGAAAT TTCTCAGGAT GAGCTGTTTG TTTTGGACCC AGAGCTGGTG 
GTCACAGAGA CTGTTAGCAC ATCACCTGGC ATGCCAGACC TGGTGGACTC TGGTGAAGTC 
CAGTCCGATC CACACAAATT TGCCTTTCCG TCATCCTCTT CTTCACCACC AAACACACCT 
GGACCTAGGG TTGCAGAGCT GCAAAGGAAG CGTGTTTCCA GTGACAGTTC GGTGGCAGAA 
GCACTTGCAC ACAGTGACTC GCTCAGATCA GCCAAGCCAG CTCTCTGCAG AGCTGGGGGT 
GTTCATCGCT CAAATACAAC CGCAGCAGAT TTCAAACCTA CCAGCAGGTT TGAGAAGAGC 
GCTGTGAACA ATGCCAACTC TGTGTCAGTG GACAGTCTAA TTGAATGTGT GAAAAAGAAG 
GACCATCAGA AGTTGAAGGA GCTGCATAAG AAAGGGGCCG ACCTGTGTGT GCAGGACCCT 
GCTGGACGGA CCCTATTGCA CTATGCTGTG GAAGTGGGCA GCAAGGAGAT TGTCAAATAC 
ATCATTGATA ATGCACCCAC CGATATCCTG GATGTAACAG AGAAAGAAAA TGGAGAGACT 
GTGTTGCACA AAGCAGCCTC GCTGTGTCAT AGGACAATCT GTCATTACCT AGTGGAAGCA 
GGAGCATCTC TTATGAAGAC AGACTTACAG GGTGACACTC CGAAGCATCG TGCCGAGAAG 
GCGAAAGATG CTGAACTTGC GGCATATCTT GAAAACCGCC AGCACTACCA AATGATCCAG 
CGTGAGGACC AGGAGACTGC AGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005169094.1
CDS	702..3788
Translation	MEQELGAGSQDREEVTASLASTSASSSNSDIPSKPLPEGDRVAVSRRHSSRTFTGLRLFGRRKAIAKSGLQHLSCQPSAAVLAKGETDREIRNTVDWSESAVYGEHIWFETNVSGDFCYVGEQHCYAKSLQKSVARKKCAACKIVVHSICIEQLEKINFRCKPSFRESGSRNIREPAIVRHHWVHRRRQEGKCKQCGKGFQQKFAFHSKEIVAISCSWCKQAYHNKVTCFMLQQIEEPCSLGAHAAVIVPPTWIIRVRRPQSSLKSSKKKKRTSFKRKSSKKGAEEARWKPFIVRPIPSQLMKPLLVFVNPKSGGNQGAKIIQTFLWYLNPRQVFDLSQGGPQEGLEMYRKVHNLRILACGGDGTVGWILSALDQLQLNPSPAVAVLPLGTGNDLARTLNWGGGYTDEPLSKILSHVEDGNIVQLDRWNLVVKPNPEAGPEERDEQVTDKLPLDVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGTAFSDFLMGSSKDLAKHIKVVCDGTDLTSKVQDLKLQCLVFLNIPRYCAGTMPWGNPSEHHDFEPQRHDDGCIEVIGFTMTSLATLQVGGHGERLNQCREVTLTTFKSIPMQVDGEPCKLAPSVIHISLRNQANMVQKTKRRTSIPLLNDSQQPFPERLRIRVSRISMHDYEALHYDKEKLKEASIPLGLIVVPGDSDLETCRAHIERLQEDFVSCNPSLQRLVLQEGDGSKSKALSSQRLSPKWCFLDSTTADRFYRIDRAQEHLNYVTEISQDELFVLDPELVVTETVSTSPGMPDLVDSGEVQSDPHKFAFPSSSSSPPNTPGPRVAELQRKRVSSDSSVAEALAHSDSLRSAKPALCRAGGVHRSNTTAADFKPTSRFEKSAVNNANSVSVDSLIECVKKKDHQKLKELHKKGADLCVQDPAGRTLLHYAVEVGSKEIVKYIIDNAPTDILDVTEKENGETVLHKAASLCHRTICHYLVEAGASLMKTDLQGDTPKHRAEKAKDAELAAYLENRQHYQMIQREDQETAV

Target ORF information:

RefSeq Version	XM_005169037.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005169037.4

ORF Insert Sequence:

ATGGAGCAAG AGCTGGGAGC AGGTTCTCAG GACAGGGAAG AGGTCACAGC CTCTCTGGCC 
TCCACGTCCG CTTCTTCATC TAACTCTGAC ATCCCATCCA AGCCTTTGCC CGAGGGGGAT 
AGAGTGGCGG TTAGTCGGCG GCACAGCAGC AGGACTTTTA CTGGGTTACG ACTGTTTGGA 
AGGAGGAAGG CTATTGCCAA GTCCGGTCTC CAGCACCTCT CATGCCAGCC CAGTGCTGCA 
GTGTTAGCCA AAGGGGAAAC GGACAGAGAA ATACGCAACA CAGTAGACTG GAGTGAATCA 
GCTGTATACG GAGAGCACAT CTGGTTTGAG ACGAATGTCT CGGGGGACTT CTGTTACGTT 
GGAGAACAGC ACTGTTACGC CAAGTCACTG CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG 
GCTTGCAAGA TCGTAGTTCA CTCTATCTGC ATTGAACAGC TGGAGAAGAT AAACTTCAGA 
TGTAAACCGT CTTTCAGGGA GTCAGGATCA CGGAACATCC GAGAGCCTGC AATAGTGCGA 
CACCACTGGG TCCATCGGAG AAGACAAGAG GGCAAATGCA AACAGTGTGG AAAGGGGTTT 
CAGCAGAAAT TTGCCTTTCA CAGTAAAGAG ATTGTAGCCA TCAGCTGCTC CTGGTGCAAA 
CAAGCATATC ATAATAAGGT GACATGCTTT ATGCTGCAGC AGATAGAAGA ACCATGTTCA 
TTAGGAGCTC ATGCCGCTGT AATAGTACCT CCTACTTGGA TCATACGGGT TCGCCGACCT 
CAGTCCTCAC TAAAGTCAAG CAAAAAGAAA AAACGGACAT CTTTCAAACG TAAATCCAGC 
AAGAAAGGAG CAGAGGAGGC ACGATGGAAG CCTTTTATAG TGAGACCCAT TCCCTCCCAA 
CTCATGAAGC CACTGCTAGT GTTTGTAAAT CCAAAGAGTG GAGGAAACCA GGGAGCTAAA 
ATCATCCAGA CCTTTCTTTG GTATCTAAAC CCTCGGCAGG TGTTTGATCT AAGTCAAGGA 
GGTCCTCAGG AAGGCTTGGA AATGTATCGC AAGGTTCACA ACTTGAGAAT TTTGGCCTGT 
GGGGGGGATG GCACGGTGGG CTGGATCTTA TCTGCACTTG ATCAGCTGCA ATTAAACCCT 
TCTCCTGCTG TGGCCGTACT TCCCCTGGGC ACAGGAAATG ACCTTGCTAG GACGCTAAAC 
TGGGGTGGGG GCTACACAGA TGAACCTCTG AGTAAAATCC TGTCTCATGT GGAGGATGGG 
AATATTGTAC AGCTGGATCG GTGGAATCTT GTTGTGAAGC CCAACCCAGA GGCAGGACCA 
GAGGAAAGAG ACGAGCAGGT GACGGACAAG CTCCCTCTAG ACGTCTTCAA TAACTACTTC 
AGCCTTGGAT TTGATGCTCA TGTGACTTTA GAGTTCCACG AATCTCGAGA GGCTAATCCT 
GAGAAATTTA ACAGCCGGTT CAGAAACAAA ATGTTCTATG CAGGGACAGC GTTTTCTGAT 
TTCTTGATGG GCAGCTCCAA AGACCTGGCT AAGCACATCA AAGTAGTCTG TGACGGCACA 
GATTTAACCT CAAAGGTCCA GGATTTGAAG CTGCAATGCC TGGTCTTCCT AAATATCCCC 
AGGTACTGTG CGGGCACAAT GCCATGGGGC AATCCGAGTG AGCATCATGA CTTTGAGCCT 
CAGCGTCATG ATGATGGCTG CATTGAGGTT ATTGGATTCA CAATGACATC ACTGGCCACA 
CTGCAGGTCG GAGGTCACGG TGAGAGGTTA AATCAGTGTC GCGAAGTCAC CCTCACCACC 
TTTAAGTCAA TCCCCATGCA GGTGGACGGG GAGCCTTGTA AACTAGCACC ATCTGTTATT 
CACATTTCTT TGCGAAACCA GGCCAACATG GTGCAGAAAA CCAAGAGACG TACATCTATA 
CCACTGCTAA ACGACAGTCA GCAACCGTTT CCAGAACGAC TACGGATCAG AGTGAGCCGT 
ATCAGCATGC ATGATTATGA AGCTTTGCAC TACGACAAGG AGAAGCTCAA AGAAGCTTCG 
ATTCCACTGG GTCTCATCGT TGTCCCTGGA GACAGTGATT TGGAAACCTG CCGAGCTCAC 
ATCGAGAGAC TACAGGAGGA CTTTGTGTCG TGTAACCCGT CTCTGCAGCG GCTGGTGCTC 
CAGGAAGGTG ACGGGAGCAA ATCAAAGGCT TTGTCGTCTC AGAGGCTATC ACCGAAATGG 
TGCTTTCTCG ACTCTACAAC TGCTGATCGA TTCTACAGGA TTGATCGAGC TCAAGAGCAT 
CTCAACTACG TGACTGAAAT TTCTCAGGAT GAGCTGTTTG TTTTGGACCC AGAGCTGGTG 
GTCACAGAGA CTGTTAGCAC ATCACCTGGC ATGCCAGACC TGGTGGACTC TGGTGAAGTC 
CAGTCCGATC CACACAAATT TGCCTTTCCG TCATCCTCTT CTTCACCACC AAACACACCT 
GGACCTAGGG TTGCAGAGCT GCAAAGGAAG CGTGTTTCCA GTGACAGTTC GGTGGCAGAA 
GCACTTGCAC ACAGTGACTC GCTCAGATCA GCCAAGCCAG CTCTCTGCAG AGCTGGGGGT 
GTTCATCGCT CAAATACAAC CGCAGCAGAT TTCAAACCTA CCAGCAGGTT TGAGAAGAGC 
GCTGTGAACA ATGCCAACTC TGTGTCAGTG GACAGTCTAA TTGAATGTGT GAAAAAGAAG 
GACCATCAGA AGTTGAAGGA GCTGCATAAG AAAGGGGCCG ACCTGTGTGT GCAGGACCCT 
GCTGGACGGA CCCTATTGCA CTATGCTGTG GAAGTGGGCA GCAAGGAGAT TGTCAAATAC 
ATCATTGATA ATGCACCCAC CGATATCCTG GATGTAACAG AGAAAGAAAA TGGAGAGACT 
GTGTTGCACA AAGCAGCCTC GCTGTGTCAT AGGACAATCT GTCATTACCT AGTGGAAGCA 
GGAGCATCTC TTATGAAGAC AGACTTACAG GGTGACACTC CGAAGCATCG TGCCGAGAAG 
GCGAAAGATG CTGAACTTGC GGCATATCTT GAAAACCGCC AGCACTACCA AATGATCCAG 
CGTGAGGACC AGGAGACTGC AGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dgkza ( XM_009303361.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301636.1 Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa41929
Clone ID Related Accession (Same CDS sequence)	XM_009303361.2
Accession Version	XM_009303361.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	diacylglycerol kinase zeta isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303361.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT 
TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG 
CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA 
GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT 
CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC 
CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC 
CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA 
TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA 
CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC 
CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT 
GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG 
ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC 
AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC 
CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA 
ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG 
ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG 
CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC 
ATTGAACAGC TGGAGAAGTT ATTGGATTGG AATGCTCCAC CTCAGGGGGA GGGGCTTGAT 
GAGATGCCTC TCAAAGAGCC GTCATTCAGA ATAAACTTCA GATGTAAACC GTCTTTCAGG 
GAGTCAGGAT CACGGAACAT CCGAGAGCCT GCAATAGTGC GACACCACTG GGTCCATCGG 
AGAAGACAAG AGGGCAAATG CAAACAGTGT GGAAAGGGGT TTCAGCAGAA ATTTGCCTTT 
CACAGTAAAG AGATTGTAGC CATCAGCTGC TCCTGGTGCA AACAAGCATA TCATAATAAG 
GTGACATGCT TTATGCTGCA GCAGATAGAA GAACCATGTT CATTAGGAGC TCATGCCGCT 
GTAATAGTAC CTCCTACTTG GATCATACGG GTTCGCCGAC CTCAGTCCTC ACTAAAGTCA 
AGCAAAAAGA AAAAACGGAC ATCTTTCAAA CGTAAATCCA GCAAGAAAGG AGCAGAGGAG 
GCACGATGGA AGCCTTTTAT AGTGAGACCC ATTCCCTCCC AACTCATGAA GCCACTGCTA 
GTGTTTGTAA ATCCAAAGAG TGGAGGAAAC CAGGGAGCTA AAATCATCCA GACCTTTCTT 
TGGTATCTAA ACCCTCGGCA GGTGTTTGAT CTAAGTCAAG GAGGTCCTCA GGAAGGCTTG 
GAAATGTATC GCAAGGTTCA CAACTTGAGA ATTTTGGCCT GTGGGGGGGA TGGCACGGTG 
GGCTGGATCT TATCTGCACT TGATCAGCTG CAATTAAACC CTTCTCCTGC TGTGGCCGTA 
CTTCCCCTGG GCACAGGAAA TGACCTTGCT AGGACGCTAA ACTGGGGTGG GGGCTACACA 
GATGAACCTC TGAGTAAAAT CCTGTCTCAT GTGGAGGATG GGAATATTGT ACAGCTGGAT 
CGGTGGAATC TTGTTGTGAA GCCCAACCCA GAGGCAGGAC CAGAGGAAAG AGACGAGCAG 
GTGACGGACA AGCTCCCTCT AGACGTCTTC AATAACTACT TCAGCCTTGG ATTTGATGCT 
CATGTGACTT TAGAGTTCCA CGAATCTCGA GAGGCTAATC CTGAGAAATT TAACAGCCGG 
TTCAGAAACA AAATGTTCTA TGCAGGGACA GCGTTTTCTG ATTTCTTGAT GGGCAGCTCC 
AAAGACCTGG CTAAGCACAT CAAAGTAGTC TGTGACGGCA CAGATTTAAC CTCAAAGGTC 
CAGGATTTGA AGCTGCAATG CCTGGTCTTC CTAAATATCC CCAGGTACTG TGCGGGCACA 
ATGCCATGGG GCAATCCGAG TGAGCATCAT GACTTTGAGC CTCAGCGTCA TGATGATGGC 
TGCATTGAGG TTATTGGATT CACAATGACA TCACTGGCCA CACTGCAGGT CGGAGGTCAC 
GGTGAGAGGT TAAATCAGTG TCGCGAAGTC ACCCTCACCA CCTTTAAGTC AATCCCCATG 
CAGGTGGACG GGGAGCCTTG TAAACTAGCA CCATCTGTTA TTCACATTTC TTTGCGAAAC 
CAGGCCAACA TGGTGCAGAA AACCAAGAGA CGTACATCTA TACCACTGCT AAACGATCAG 
CAACCGTTTC CAGAACGACT ACGGATCAGA GTGAGCCGTA TCAGCATGCA TGATTATGAA 
GCTTTGCACT ACGACAAGGA GAAGCTCAAA GAAGCTTCGA TTCCACTGGG TCTCATCGTT 
GTCCCTGGAG ACAGTGATTT GGAAACCTGC CGAGCTCACA TCGAGAGACT ACAGGAGGAC 
TTTGTGTCGT GTAACCCGTC TCTGCAGCGG CTGGTGCTCC AGGAAGGTGA CGGGAGCAAA 
TCAAAGGCTT TGTCGTCTCA GAGGCTATCA CCGAAATGGT GCTTTCTCGA CTCTACAACT 
GCTGATCGAT TCTACAGGAT TGATCGAGCT CAAGAGCATC TCAACTACGT GACTGAAATT 
TCTCAGGATG AGCTGTTTGT TTTGGACCCA GAGCTGGTGG TCACAGAGAC TGTTAGCACA 
TCACCTGGCA TGCCAGACCT GGTGGACTCT GGTGAAGTCC AGTCCGATCC ACACAAATTT 
GCCTTTCCGT CATCCTCTTC TTCACCACCA AACACACCTG GACCTAGGGT TGCAGAGCTG 
CAAAGGAAGC GTGTTTCCAG TGACAGTTCG GTGGCAGAAG CACTTGCACA CAGTGACTCG 
CTCAGATCAG CCAAGCCAGC TCTCTGCAGA GCTGGGGGTG TTCATCGCTC AAATACAACC 
GCAGCAGATT TCAAACCTAC CAGCAGGTTT GAGAAGAGCG CTGTGAACAA TGCCAACTCT 
GTGTCAGTGG ACAGTCTAAT TGAATGTGTG AAAAAGAAGG ACCATCAGAA GTTGAAGGAG 
CTGCATAAGA AAGGGGCCGA CCTGTGTGTG CAGGACCCTG CTGGACGGAC CCTATTGCAC 
TATGCTGTGG AAGTGGGCAG CAAGGAGATT GTCAAATACA TCATTGATAA TGCACCCACC 
GATATCCTGG ATGTAACAGA GAAAGAAAAT GGAGAGACTG TGTTGCACAA AGCAGCCTCG 
CTGTGTCATA GGACAATCTG TCATTACCTA GTGGAAGCAG GAGCATCTCT TATGAAGACA 
GACTTACAGG GTGACACTCC GAAGCATCGT GCCGAGAAGG CGAAAGATGC TGAACTTGCG 
GCATATCTTG AAAACCGCCA GCACTACCAA ATGATCCAGC GTGAGGACCA GGAGACTGCA 
GTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301636.1
CDS	136..3921
Translation	MDALFNLRFRRKAVEPSGRRRSSVSLPASKARRRSSAGLPSAGLAHRRRSSAQLQIGSIQPGLVLHRRGHFTRRKSSGGTACLTPRFAIRRRQAAKHRSIHAQLLGPSLLLASVVQMSEDHEPEDKSAEEYSSLSDTDCSQDEHEQTGMDFETESDKCLTWLAQGLLVGESIQPRPLIRAPRCLRRNSSHLLPAEAVYRSPASYGLYGRYRRTSQVHNMGGLWAGKRSSLAARGSVALYRTSPPCWPDLSVSHTLQGTYYNPQIDTWSGFLSKAIAKSGLQHLSCQPSAAVLAKGETDREIRNTVDWSESAVYGEHIWFETNVSGDFCYVGEQHCYAKSLQKSVARKKCAACKIVVHSICIEQLEKLLDWNAPPQGEGLDEMPLKEPSFRINFRCKPSFRESGSRNIREPAIVRHHWVHRRRQEGKCKQCGKGFQQKFAFHSKEIVAISCSWCKQAYHNKVTCFMLQQIEEPCSLGAHAAVIVPPTWIIRVRRPQSSLKSSKKKKRTSFKRKSSKKGAEEARWKPFIVRPIPSQLMKPLLVFVNPKSGGNQGAKIIQTFLWYLNPRQVFDLSQGGPQEGLEMYRKVHNLRILACGGDGTVGWILSALDQLQLNPSPAVAVLPLGTGNDLARTLNWGGGYTDEPLSKILSHVEDGNIVQLDRWNLVVKPNPEAGPEERDEQVTDKLPLDVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGTAFSDFLMGSSKDLAKHIKVVCDGTDLTSKVQDLKLQCLVFLNIPRYCAGTMPWGNPSEHHDFEPQRHDDGCIEVIGFTMTSLATLQVGGHGERLNQCREVTLTTFKSIPMQVDGEPCKLAPSVIHISLRNQANMVQKTKRRTSIPLLNDQQPFPERLRIRVSRISMHDYEALHYDKEKLKEASIPLGLIVVPGDSDLETCRAHIERLQEDFVSCNPSLQRLVLQEGDGSKSKALSSQRLSPKWCFLDSTTADRFYRIDRAQEHLNYVTEISQDELFVLDPELVVTETVSTSPGMPDLVDSGEVQSDPHKFAFPSSSSSPPNTPGPRVAELQRKRVSSDSSVAEALAHSDSLRSAKPALCRAGGVHRSNTTAADFKPTSRFEKSAVNNANSVSVDSLIECVKKKDHQKLKELHKKGADLCVQDPAGRTLLHYAVEVGSKEIVKYIIDNAPTDILDVTEKENGETVLHKAASLCHRTICHYLVEAGASLMKTDLQGDTPKHRAEKAKDAELAAYLENRQHYQMIQREDQETAV

Target ORF information:

RefSeq Version	XM_009303361.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303361.2

ORF Insert Sequence:

ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT 
TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG 
CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA 
GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT 
CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC 
CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC 
CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA 
TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA 
CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC 
CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT 
GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG 
ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC 
AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC 
CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA 
ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG 
ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG 
CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC 
ATTGAACAGC TGGAGAAGTT ATTGGATTGG AATGCTCCAC CTCAGGGGGA GGGGCTTGAT 
GAGATGCCTC TCAAAGAGCC GTCATTCAGA ATAAACTTCA GATGTAAACC GTCTTTCAGG 
GAGTCAGGAT CACGGAACAT CCGAGAGCCT GCAATAGTGC GACACCACTG GGTCCATCGG 
AGAAGACAAG AGGGCAAATG CAAACAGTGT GGAAAGGGGT TTCAGCAGAA ATTTGCCTTT 
CACAGTAAAG AGATTGTAGC CATCAGCTGC TCCTGGTGCA AACAAGCATA TCATAATAAG 
GTGACATGCT TTATGCTGCA GCAGATAGAA GAACCATGTT CATTAGGAGC TCATGCCGCT 
GTAATAGTAC CTCCTACTTG GATCATACGG GTTCGCCGAC CTCAGTCCTC ACTAAAGTCA 
AGCAAAAAGA AAAAACGGAC ATCTTTCAAA CGTAAATCCA GCAAGAAAGG AGCAGAGGAG 
GCACGATGGA AGCCTTTTAT AGTGAGACCC ATTCCCTCCC AACTCATGAA GCCACTGCTA 
GTGTTTGTAA ATCCAAAGAG TGGAGGAAAC CAGGGAGCTA AAATCATCCA GACCTTTCTT 
TGGTATCTAA ACCCTCGGCA GGTGTTTGAT CTAAGTCAAG GAGGTCCTCA GGAAGGCTTG 
GAAATGTATC GCAAGGTTCA CAACTTGAGA ATTTTGGCCT GTGGGGGGGA TGGCACGGTG 
GGCTGGATCT TATCTGCACT TGATCAGCTG CAATTAAACC CTTCTCCTGC TGTGGCCGTA 
CTTCCCCTGG GCACAGGAAA TGACCTTGCT AGGACGCTAA ACTGGGGTGG GGGCTACACA 
GATGAACCTC TGAGTAAAAT CCTGTCTCAT GTGGAGGATG GGAATATTGT ACAGCTGGAT 
CGGTGGAATC TTGTTGTGAA GCCCAACCCA GAGGCAGGAC CAGAGGAAAG AGACGAGCAG 
GTGACGGACA AGCTCCCTCT AGACGTCTTC AATAACTACT TCAGCCTTGG ATTTGATGCT 
CATGTGACTT TAGAGTTCCA CGAATCTCGA GAGGCTAATC CTGAGAAATT TAACAGCCGG 
TTCAGAAACA AAATGTTCTA TGCAGGGACA GCGTTTTCTG ATTTCTTGAT GGGCAGCTCC 
AAAGACCTGG CTAAGCACAT CAAAGTAGTC TGTGACGGCA CAGATTTAAC CTCAAAGGTC 
CAGGATTTGA AGCTGCAATG CCTGGTCTTC CTAAATATCC CCAGGTACTG TGCGGGCACA 
ATGCCATGGG GCAATCCGAG TGAGCATCAT GACTTTGAGC CTCAGCGTCA TGATGATGGC 
TGCATTGAGG TTATTGGATT CACAATGACA TCACTGGCCA CACTGCAGGT CGGAGGTCAC 
GGTGAGAGGT TAAATCAGTG TCGCGAAGTC ACCCTCACCA CCTTTAAGTC AATCCCCATG 
CAGGTGGACG GGGAGCCTTG TAAACTAGCA CCATCTGTTA TTCACATTTC TTTGCGAAAC 
CAGGCCAACA TGGTGCAGAA AACCAAGAGA CGTACATCTA TACCACTGCT AAACGATCAG 
CAACCGTTTC CAGAACGACT ACGGATCAGA GTGAGCCGTA TCAGCATGCA TGATTATGAA 
GCTTTGCACT ACGACAAGGA GAAGCTCAAA GAAGCTTCGA TTCCACTGGG TCTCATCGTT 
GTCCCTGGAG ACAGTGATTT GGAAACCTGC CGAGCTCACA TCGAGAGACT ACAGGAGGAC 
TTTGTGTCGT GTAACCCGTC TCTGCAGCGG CTGGTGCTCC AGGAAGGTGA CGGGAGCAAA 
TCAAAGGCTT TGTCGTCTCA GAGGCTATCA CCGAAATGGT GCTTTCTCGA CTCTACAACT 
GCTGATCGAT TCTACAGGAT TGATCGAGCT CAAGAGCATC TCAACTACGT GACTGAAATT 
TCTCAGGATG AGCTGTTTGT TTTGGACCCA GAGCTGGTGG TCACAGAGAC TGTTAGCACA 
TCACCTGGCA TGCCAGACCT GGTGGACTCT GGTGAAGTCC AGTCCGATCC ACACAAATTT 
GCCTTTCCGT CATCCTCTTC TTCACCACCA AACACACCTG GACCTAGGGT TGCAGAGCTG 
CAAAGGAAGC GTGTTTCCAG TGACAGTTCG GTGGCAGAAG CACTTGCACA CAGTGACTCG 
CTCAGATCAG CCAAGCCAGC TCTCTGCAGA GCTGGGGGTG TTCATCGCTC AAATACAACC 
GCAGCAGATT TCAAACCTAC CAGCAGGTTT GAGAAGAGCG CTGTGAACAA TGCCAACTCT 
GTGTCAGTGG ACAGTCTAAT TGAATGTGTG AAAAAGAAGG ACCATCAGAA GTTGAAGGAG 
CTGCATAAGA AAGGGGCCGA CCTGTGTGTG CAGGACCCTG CTGGACGGAC CCTATTGCAC 
TATGCTGTGG AAGTGGGCAG CAAGGAGATT GTCAAATACA TCATTGATAA TGCACCCACC 
GATATCCTGG ATGTAACAGA GAAAGAAAAT GGAGAGACTG TGTTGCACAA AGCAGCCTCG 
CTGTGTCATA GGACAATCTG TCATTACCTA GTGGAAGCAG GAGCATCTCT TATGAAGACA 
GACTTACAGG GTGACACTCC GAAGCATCGT GCCGAGAAGG CGAAAGATGC TGAACTTGCG 
GCATATCTTG AAAACCGCCA GCACTACCAA ATGATCCAGC GTGAGGACCA GGAGACTGCA 
GTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dgkza ( XM_009303362.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301637.1 Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X4, mRNA.

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CloneID	ODa41930
Clone ID Related Accession (Same CDS sequence)	XM_009303362.3 , XM_009303362.2
Accession Version	XM_009303362.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	diacylglycerol kinase zeta isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303362.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT 
TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG 
CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA 
GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT 
CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC 
CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC 
CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA 
TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA 
CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC 
CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT 
GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG 
ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC 
AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC 
CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA 
ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG 
ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG 
CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC 
ATTGAACAGC TGGAGAAGAT AAACTTCAGA TGTAAACCGT CTTTCAGGGA GTCAGGATCA 
CGGAACATCC GAGAGCCTGC AATAGTGCGA CACCACTGGG TCCATCGGAG AAGACAAGAG 
GGCAAATGCA AACAGTGTGG AAAGGGGTTT CAGCAGAAAT TTGCCTTTCA CAGTAAAGAG 
ATTGTAGCCA TCAGCTGCTC CTGGTGCAAA CAAGCATATC ATAATAAGGT GACATGCTTT 
ATGCTGCAGC AGATAGAAGA ACCATGTTCA TTAGGAGCTC ATGCCGCTGT AATAGTACCT 
CCTACTTGGA TCATACGGGT TCGCCGACCT CAGTCCTCAC TAAAGTCAAG CAAAAAGAAA 
AAACGGACAT CTTTCAAACG TAAATCCAGC AAGAAAGGAG CAGAGGAGGC ACGATGGAAG 
CCTTTTATAG TGAGACCCAT TCCCTCCCAA CTCATGAAGC CACTGCTAGT GTTTGTAAAT 
CCAAAGAGTG GAGGAAACCA GGGAGCTAAA ATCATCCAGA CCTTTCTTTG GTATCTAAAC 
CCTCGGCAGG TGTTTGATCT AAGTCAAGGA GGTCCTCAGG AAGGCTTGGA AATGTATCGC 
AAGGTTCACA ACTTGAGAAT TTTGGCCTGT GGGGGGGATG GCACGGTGGG CTGGATCTTA 
TCTGCACTTG ATCAGCTGCA ATTAAACCCT TCTCCTGCTG TGGCCGTACT TCCCCTGGGC 
ACAGGAAATG ACCTTGCTAG GACGCTAAAC TGGGGTGGGG GCTACACAGA TGAACCTCTG 
AGTAAAATCC TGTCTCATGT GGAGGATGGG AATATTGTAC AGCTGGATCG GTGGAATCTT 
GTTGTGAAGC CCAACCCAGA GGCAGGACCA GAGGAAAGAG ACGAGCAGGT GACGGACAAG 
CTCCCTCTAG ACGTCTTCAA TAACTACTTC AGCCTTGGAT TTGATGCTCA TGTGACTTTA 
GAGTTCCACG AATCTCGAGA GGCTAATCCT GAGAAATTTA ACAGCCGGTT CAGAAACAAA 
ATGTTCTATG CAGGGACAGC GTTTTCTGAT TTCTTGATGG GCAGCTCCAA AGACCTGGCT 
AAGCACATCA AAGTAGTCTG TGACGGCACA GATTTAACCT CAAAGGTCCA GGATTTGAAG 
CTGCAATGCC TGGTCTTCCT AAATATCCCC AGGTACTGTG CGGGCACAAT GCCATGGGGC 
AATCCGAGTG AGCATCATGA CTTTGAGCCT CAGCGTCATG ATGATGGCTG CATTGAGGTT 
ATTGGATTCA CAATGACATC ACTGGCCACA CTGCAGGTCG GAGGTCACGG TGAGAGGTTA 
AATCAGTGTC GCGAAGTCAC CCTCACCACC TTTAAGTCAA TCCCCATGCA GGTGGACGGG 
GAGCCTTGTA AACTAGCACC ATCTGTTATT CACATTTCTT TGCGAAACCA GGCCAACATG 
GTGCAGAAAA CCAAGAGACG TACATCTATA CCACTGCTAA ACGATCAGCA ACCGTTTCCA 
GAACGACTAC GGATCAGAGT GAGCCGTATC AGCATGCATG ATTATGAAGC TTTGCACTAC 
GACAAGGAGA AGCTCAAAGA AGCTTCGATT CCACTGGGTC TCATCGTTGT CCCTGGAGAC 
AGTGATTTGG AAACCTGCCG AGCTCACATC GAGAGACTAC AGGAGGACTT TGTGTCGTGT 
AACCCGTCTC TGCAGCGGCT GGTGCTCCAG GAAGGTGACG GGAGCAAATC AAAGGCTTTG 
TCGTCTCAGA GGCTATCACC GAAATGGTGC TTTCTCGACT CTACAACTGC TGATCGATTC 
TACAGGATTG ATCGAGCTCA AGAGCATCTC AACTACGTGA CTGAAATTTC TCAGGATGAG 
CTGTTTGTTT TGGACCCAGA GCTGGTGGTC ACAGAGACTG TTAGCACATC ACCTGGCATG 
CCAGACCTGG TGGACTCTGG TGAAGTCCAG TCCGATCCAC ACAAATTTGC CTTTCCGTCA 
TCCTCTTCTT CACCACCAAA CACACCTGGA CCTAGGGTTG CAGAGCTGCA AAGGAAGCGT 
GTTTCCAGTG ACAGTTCGGT GGCAGAAGCA CTTGCACACA GTGACTCGCT CAGATCAGCC 
AAGCCAGCTC TCTGCAGAGC TGGGGGTGTT CATCGCTCAA ATACAACCGC AGCAGATTTC 
AAACCTACCA GCAGGTTTGA GAAGAGCGCT GTGAACAATG CCAACTCTGT GTCAGTGGAC 
AGTCTAATTG AATGTGTGAA AAAGAAGGAC CATCAGAAGT TGAAGGAGCT GCATAAGAAA 
GGGGCCGACC TGTGTGTGCA GGACCCTGCT GGACGGACCC TATTGCACTA TGCTGTGGAA 
GTGGGCAGCA AGGAGATTGT CAAATACATC ATTGATAATG CACCCACCGA TATCCTGGAT 
GTAACAGAGA AAGAAAATGG AGAGACTGTG TTGCACAAAG CAGCCTCGCT GTGTCATAGG 
ACAATCTGTC ATTACCTAGT GGAAGCAGGA GCATCTCTTA TGAAGACAGA CTTACAGGGT 
GACACTCCGA AGCATCGTGC CGAGAAGGCG AAAGATGCTG AACTTGCGGC ATATCTTGAA 
AACCGCCAGC ACTACCAAAT GATCCAGCGT GAGGACCAGG AGACTGCAGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301637.1
CDS	170..3883
Translation	MDALFNLRFRRKAVEPSGRRRSSVSLPASKARRRSSAGLPSAGLAHRRRSSAQLQIGSIQPGLVLHRRGHFTRRKSSGGTACLTPRFAIRRRQAAKHRSIHAQLLGPSLLLASVVQMSEDHEPEDKSAEEYSSLSDTDCSQDEHEQTGMDFETESDKCLTWLAQGLLVGESIQPRPLIRAPRCLRRNSSHLLPAEAVYRSPASYGLYGRYRRTSQVHNMGGLWAGKRSSLAARGSVALYRTSPPCWPDLSVSHTLQGTYYNPQIDTWSGFLSKAIAKSGLQHLSCQPSAAVLAKGETDREIRNTVDWSESAVYGEHIWFETNVSGDFCYVGEQHCYAKSLQKSVARKKCAACKIVVHSICIEQLEKINFRCKPSFRESGSRNIREPAIVRHHWVHRRRQEGKCKQCGKGFQQKFAFHSKEIVAISCSWCKQAYHNKVTCFMLQQIEEPCSLGAHAAVIVPPTWIIRVRRPQSSLKSSKKKKRTSFKRKSSKKGAEEARWKPFIVRPIPSQLMKPLLVFVNPKSGGNQGAKIIQTFLWYLNPRQVFDLSQGGPQEGLEMYRKVHNLRILACGGDGTVGWILSALDQLQLNPSPAVAVLPLGTGNDLARTLNWGGGYTDEPLSKILSHVEDGNIVQLDRWNLVVKPNPEAGPEERDEQVTDKLPLDVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGTAFSDFLMGSSKDLAKHIKVVCDGTDLTSKVQDLKLQCLVFLNIPRYCAGTMPWGNPSEHHDFEPQRHDDGCIEVIGFTMTSLATLQVGGHGERLNQCREVTLTTFKSIPMQVDGEPCKLAPSVIHISLRNQANMVQKTKRRTSIPLLNDQQPFPERLRIRVSRISMHDYEALHYDKEKLKEASIPLGLIVVPGDSDLETCRAHIERLQEDFVSCNPSLQRLVLQEGDGSKSKALSSQRLSPKWCFLDSTTADRFYRIDRAQEHLNYVTEISQDELFVLDPELVVTETVSTSPGMPDLVDSGEVQSDPHKFAFPSSSSSPPNTPGPRVAELQRKRVSSDSSVAEALAHSDSLRSAKPALCRAGGVHRSNTTAADFKPTSRFEKSAVNNANSVSVDSLIECVKKKDHQKLKELHKKGADLCVQDPAGRTLLHYAVEVGSKEIVKYIIDNAPTDILDVTEKENGETVLHKAASLCHRTICHYLVEAGASLMKTDLQGDTPKHRAEKAKDAELAAYLENRQHYQMIQREDQETAV

Target ORF information:

RefSeq Version	XM_009303362.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303362.3

ORF Insert Sequence:

ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT 
TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG 
CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA 
GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT 
CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC 
CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC 
CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA 
TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA 
CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC 
CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT 
GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG 
ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC 
AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC 
CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA 
ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG 
ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG 
CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC 
ATTGAACAGC TGGAGAAGAT AAACTTCAGA TGTAAACCGT CTTTCAGGGA GTCAGGATCA 
CGGAACATCC GAGAGCCTGC AATAGTGCGA CACCACTGGG TCCATCGGAG AAGACAAGAG 
GGCAAATGCA AACAGTGTGG AAAGGGGTTT CAGCAGAAAT TTGCCTTTCA CAGTAAAGAG 
ATTGTAGCCA TCAGCTGCTC CTGGTGCAAA CAAGCATATC ATAATAAGGT GACATGCTTT 
ATGCTGCAGC AGATAGAAGA ACCATGTTCA TTAGGAGCTC ATGCCGCTGT AATAGTACCT 
CCTACTTGGA TCATACGGGT TCGCCGACCT CAGTCCTCAC TAAAGTCAAG CAAAAAGAAA 
AAACGGACAT CTTTCAAACG TAAATCCAGC AAGAAAGGAG CAGAGGAGGC ACGATGGAAG 
CCTTTTATAG TGAGACCCAT TCCCTCCCAA CTCATGAAGC CACTGCTAGT GTTTGTAAAT 
CCAAAGAGTG GAGGAAACCA GGGAGCTAAA ATCATCCAGA CCTTTCTTTG GTATCTAAAC 
CCTCGGCAGG TGTTTGATCT AAGTCAAGGA GGTCCTCAGG AAGGCTTGGA AATGTATCGC 
AAGGTTCACA ACTTGAGAAT TTTGGCCTGT GGGGGGGATG GCACGGTGGG CTGGATCTTA 
TCTGCACTTG ATCAGCTGCA ATTAAACCCT TCTCCTGCTG TGGCCGTACT TCCCCTGGGC 
ACAGGAAATG ACCTTGCTAG GACGCTAAAC TGGGGTGGGG GCTACACAGA TGAACCTCTG 
AGTAAAATCC TGTCTCATGT GGAGGATGGG AATATTGTAC AGCTGGATCG GTGGAATCTT 
GTTGTGAAGC CCAACCCAGA GGCAGGACCA GAGGAAAGAG ACGAGCAGGT GACGGACAAG 
CTCCCTCTAG ACGTCTTCAA TAACTACTTC AGCCTTGGAT TTGATGCTCA TGTGACTTTA 
GAGTTCCACG AATCTCGAGA GGCTAATCCT GAGAAATTTA ACAGCCGGTT CAGAAACAAA 
ATGTTCTATG CAGGGACAGC GTTTTCTGAT TTCTTGATGG GCAGCTCCAA AGACCTGGCT 
AAGCACATCA AAGTAGTCTG TGACGGCACA GATTTAACCT CAAAGGTCCA GGATTTGAAG 
CTGCAATGCC TGGTCTTCCT AAATATCCCC AGGTACTGTG CGGGCACAAT GCCATGGGGC 
AATCCGAGTG AGCATCATGA CTTTGAGCCT CAGCGTCATG ATGATGGCTG CATTGAGGTT 
ATTGGATTCA CAATGACATC ACTGGCCACA CTGCAGGTCG GAGGTCACGG TGAGAGGTTA 
AATCAGTGTC GCGAAGTCAC CCTCACCACC TTTAAGTCAA TCCCCATGCA GGTGGACGGG 
GAGCCTTGTA AACTAGCACC ATCTGTTATT CACATTTCTT TGCGAAACCA GGCCAACATG 
GTGCAGAAAA CCAAGAGACG TACATCTATA CCACTGCTAA ACGATCAGCA ACCGTTTCCA 
GAACGACTAC GGATCAGAGT GAGCCGTATC AGCATGCATG ATTATGAAGC TTTGCACTAC 
GACAAGGAGA AGCTCAAAGA AGCTTCGATT CCACTGGGTC TCATCGTTGT CCCTGGAGAC 
AGTGATTTGG AAACCTGCCG AGCTCACATC GAGAGACTAC AGGAGGACTT TGTGTCGTGT 
AACCCGTCTC TGCAGCGGCT GGTGCTCCAG GAAGGTGACG GGAGCAAATC AAAGGCTTTG 
TCGTCTCAGA GGCTATCACC GAAATGGTGC TTTCTCGACT CTACAACTGC TGATCGATTC 
TACAGGATTG ATCGAGCTCA AGAGCATCTC AACTACGTGA CTGAAATTTC TCAGGATGAG 
CTGTTTGTTT TGGACCCAGA GCTGGTGGTC ACAGAGACTG TTAGCACATC ACCTGGCATG 
CCAGACCTGG TGGACTCTGG TGAAGTCCAG TCCGATCCAC ACAAATTTGC CTTTCCGTCA 
TCCTCTTCTT CACCACCAAA CACACCTGGA CCTAGGGTTG CAGAGCTGCA AAGGAAGCGT 
GTTTCCAGTG ACAGTTCGGT GGCAGAAGCA CTTGCACACA GTGACTCGCT CAGATCAGCC 
AAGCCAGCTC TCTGCAGAGC TGGGGGTGTT CATCGCTCAA ATACAACCGC AGCAGATTTC 
AAACCTACCA GCAGGTTTGA GAAGAGCGCT GTGAACAATG CCAACTCTGT GTCAGTGGAC 
AGTCTAATTG AATGTGTGAA AAAGAAGGAC CATCAGAAGT TGAAGGAGCT GCATAAGAAA 
GGGGCCGACC TGTGTGTGCA GGACCCTGCT GGACGGACCC TATTGCACTA TGCTGTGGAA 
GTGGGCAGCA AGGAGATTGT CAAATACATC ATTGATAATG CACCCACCGA TATCCTGGAT 
GTAACAGAGA AAGAAAATGG AGAGACTGTG TTGCACAAAG CAGCCTCGCT GTGTCATAGG 
ACAATCTGTC ATTACCTAGT GGAAGCAGGA GCATCTCTTA TGAAGACAGA CTTACAGGGT 
GACACTCCGA AGCATCGTGC CGAGAAGGCG AAAGATGCTG AACTTGCGGC ATATCTTGAA 
AACCGCCAGC ACTACCAAAT GATCCAGCGT GAGGACCAGG AGACTGCAGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dgkza ( XM_009303362.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301637.1 Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X7, mRNA.

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CloneID	ODa41930
Clone ID Related Accession (Same CDS sequence)	XM_009303362.3 , XM_009303362.2
Accession Version	XM_009303362.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	diacylglycerol kinase zeta isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303362.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT 
TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG 
CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA 
GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT 
CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC 
CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC 
CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA 
TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA 
CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC 
CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT 
GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG 
ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC 
AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC 
CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA 
ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG 
ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG 
CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC 
ATTGAACAGC TGGAGAAGAT AAACTTCAGA TGTAAACCGT CTTTCAGGGA GTCAGGATCA 
CGGAACATCC GAGAGCCTGC AATAGTGCGA CACCACTGGG TCCATCGGAG AAGACAAGAG 
GGCAAATGCA AACAGTGTGG AAAGGGGTTT CAGCAGAAAT TTGCCTTTCA CAGTAAAGAG 
ATTGTAGCCA TCAGCTGCTC CTGGTGCAAA CAAGCATATC ATAATAAGGT GACATGCTTT 
ATGCTGCAGC AGATAGAAGA ACCATGTTCA TTAGGAGCTC ATGCCGCTGT AATAGTACCT 
CCTACTTGGA TCATACGGGT TCGCCGACCT CAGTCCTCAC TAAAGTCAAG CAAAAAGAAA 
AAACGGACAT CTTTCAAACG TAAATCCAGC AAGAAAGGAG CAGAGGAGGC ACGATGGAAG 
CCTTTTATAG TGAGACCCAT TCCCTCCCAA CTCATGAAGC CACTGCTAGT GTTTGTAAAT 
CCAAAGAGTG GAGGAAACCA GGGAGCTAAA ATCATCCAGA CCTTTCTTTG GTATCTAAAC 
CCTCGGCAGG TGTTTGATCT AAGTCAAGGA GGTCCTCAGG AAGGCTTGGA AATGTATCGC 
AAGGTTCACA ACTTGAGAAT TTTGGCCTGT GGGGGGGATG GCACGGTGGG CTGGATCTTA 
TCTGCACTTG ATCAGCTGCA ATTAAACCCT TCTCCTGCTG TGGCCGTACT TCCCCTGGGC 
ACAGGAAATG ACCTTGCTAG GACGCTAAAC TGGGGTGGGG GCTACACAGA TGAACCTCTG 
AGTAAAATCC TGTCTCATGT GGAGGATGGG AATATTGTAC AGCTGGATCG GTGGAATCTT 
GTTGTGAAGC CCAACCCAGA GGCAGGACCA GAGGAAAGAG ACGAGCAGGT GACGGACAAG 
CTCCCTCTAG ACGTCTTCAA TAACTACTTC AGCCTTGGAT TTGATGCTCA TGTGACTTTA 
GAGTTCCACG AATCTCGAGA GGCTAATCCT GAGAAATTTA ACAGCCGGTT CAGAAACAAA 
ATGTTCTATG CAGGGACAGC GTTTTCTGAT TTCTTGATGG GCAGCTCCAA AGACCTGGCT 
AAGCACATCA AAGTAGTCTG TGACGGCACA GATTTAACCT CAAAGGTCCA GGATTTGAAG 
CTGCAATGCC TGGTCTTCCT AAATATCCCC AGGTACTGTG CGGGCACAAT GCCATGGGGC 
AATCCGAGTG AGCATCATGA CTTTGAGCCT CAGCGTCATG ATGATGGCTG CATTGAGGTT 
ATTGGATTCA CAATGACATC ACTGGCCACA CTGCAGGTCG GAGGTCACGG TGAGAGGTTA 
AATCAGTGTC GCGAAGTCAC CCTCACCACC TTTAAGTCAA TCCCCATGCA GGTGGACGGG 
GAGCCTTGTA AACTAGCACC ATCTGTTATT CACATTTCTT TGCGAAACCA GGCCAACATG 
GTGCAGAAAA CCAAGAGACG TACATCTATA CCACTGCTAA ACGATCAGCA ACCGTTTCCA 
GAACGACTAC GGATCAGAGT GAGCCGTATC AGCATGCATG ATTATGAAGC TTTGCACTAC 
GACAAGGAGA AGCTCAAAGA AGCTTCGATT CCACTGGGTC TCATCGTTGT CCCTGGAGAC 
AGTGATTTGG AAACCTGCCG AGCTCACATC GAGAGACTAC AGGAGGACTT TGTGTCGTGT 
AACCCGTCTC TGCAGCGGCT GGTGCTCCAG GAAGGTGACG GGAGCAAATC AAAGGCTTTG 
TCGTCTCAGA GGCTATCACC GAAATGGTGC TTTCTCGACT CTACAACTGC TGATCGATTC 
TACAGGATTG ATCGAGCTCA AGAGCATCTC AACTACGTGA CTGAAATTTC TCAGGATGAG 
CTGTTTGTTT TGGACCCAGA GCTGGTGGTC ACAGAGACTG TTAGCACATC ACCTGGCATG 
CCAGACCTGG TGGACTCTGG TGAAGTCCAG TCCGATCCAC ACAAATTTGC CTTTCCGTCA 
TCCTCTTCTT CACCACCAAA CACACCTGGA CCTAGGGTTG CAGAGCTGCA AAGGAAGCGT 
GTTTCCAGTG ACAGTTCGGT GGCAGAAGCA CTTGCACACA GTGACTCGCT CAGATCAGCC 
AAGCCAGCTC TCTGCAGAGC TGGGGGTGTT CATCGCTCAA ATACAACCGC AGCAGATTTC 
AAACCTACCA GCAGGTTTGA GAAGAGCGCT GTGAACAATG CCAACTCTGT GTCAGTGGAC 
AGTCTAATTG AATGTGTGAA AAAGAAGGAC CATCAGAAGT TGAAGGAGCT GCATAAGAAA 
GGGGCCGACC TGTGTGTGCA GGACCCTGCT GGACGGACCC TATTGCACTA TGCTGTGGAA 
GTGGGCAGCA AGGAGATTGT CAAATACATC ATTGATAATG CACCCACCGA TATCCTGGAT 
GTAACAGAGA AAGAAAATGG AGAGACTGTG TTGCACAAAG CAGCCTCGCT GTGTCATAGG 
ACAATCTGTC ATTACCTAGT GGAAGCAGGA GCATCTCTTA TGAAGACAGA CTTACAGGGT 
GACACTCCGA AGCATCGTGC CGAGAAGGCG AAAGATGCTG AACTTGCGGC ATATCTTGAA 
AACCGCCAGC ACTACCAAAT GATCCAGCGT GAGGACCAGG AGACTGCAGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301637.1
CDS	136..3849
Translation	MDALFNLRFRRKAVEPSGRRRSSVSLPASKARRRSSAGLPSAGLAHRRRSSAQLQIGSIQPGLVLHRRGHFTRRKSSGGTACLTPRFAIRRRQAAKHRSIHAQLLGPSLLLASVVQMSEDHEPEDKSAEEYSSLSDTDCSQDEHEQTGMDFETESDKCLTWLAQGLLVGESIQPRPLIRAPRCLRRNSSHLLPAEAVYRSPASYGLYGRYRRTSQVHNMGGLWAGKRSSLAARGSVALYRTSPPCWPDLSVSHTLQGTYYNPQIDTWSGFLSKAIAKSGLQHLSCQPSAAVLAKGETDREIRNTVDWSESAVYGEHIWFETNVSGDFCYVGEQHCYAKSLQKSVARKKCAACKIVVHSICIEQLEKINFRCKPSFRESGSRNIREPAIVRHHWVHRRRQEGKCKQCGKGFQQKFAFHSKEIVAISCSWCKQAYHNKVTCFMLQQIEEPCSLGAHAAVIVPPTWIIRVRRPQSSLKSSKKKKRTSFKRKSSKKGAEEARWKPFIVRPIPSQLMKPLLVFVNPKSGGNQGAKIIQTFLWYLNPRQVFDLSQGGPQEGLEMYRKVHNLRILACGGDGTVGWILSALDQLQLNPSPAVAVLPLGTGNDLARTLNWGGGYTDEPLSKILSHVEDGNIVQLDRWNLVVKPNPEAGPEERDEQVTDKLPLDVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGTAFSDFLMGSSKDLAKHIKVVCDGTDLTSKVQDLKLQCLVFLNIPRYCAGTMPWGNPSEHHDFEPQRHDDGCIEVIGFTMTSLATLQVGGHGERLNQCREVTLTTFKSIPMQVDGEPCKLAPSVIHISLRNQANMVQKTKRRTSIPLLNDQQPFPERLRIRVSRISMHDYEALHYDKEKLKEASIPLGLIVVPGDSDLETCRAHIERLQEDFVSCNPSLQRLVLQEGDGSKSKALSSQRLSPKWCFLDSTTADRFYRIDRAQEHLNYVTEISQDELFVLDPELVVTETVSTSPGMPDLVDSGEVQSDPHKFAFPSSSSSPPNTPGPRVAELQRKRVSSDSSVAEALAHSDSLRSAKPALCRAGGVHRSNTTAADFKPTSRFEKSAVNNANSVSVDSLIECVKKKDHQKLKELHKKGADLCVQDPAGRTLLHYAVEVGSKEIVKYIIDNAPTDILDVTEKENGETVLHKAASLCHRTICHYLVEAGASLMKTDLQGDTPKHRAEKAKDAELAAYLENRQHYQMIQREDQETAV

Target ORF information:

RefSeq Version	XM_009303362.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio diacylglycerol kinase, zeta a (dgkza), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303362.2

ORF Insert Sequence:

ATGGATGCTT TGTTCAACCT GCGTTTCAGG CGGAAAGCCG TTGAGCCAAG CGGGCGCAGG 
CGCTCCAGCG TGTCACTTCC AGCAAGCAAA GCACGTCGGC GCTCCAGCGC AGGTCTGCCT 
TCAGCAGGAC TGGCCCACAG ACGCAGATCC AGTGCTCAGC TTCAAATCGG CAGCATACAG 
CCAGGGCTCG TACTACACAG AAGAGGACAC TTTACGAGGA GAAAATCAAG TGGTGGGACA 
GCCTGCCTGA CTCCGCGGTT CGCTATCCGC CGAAGACAGG CTGCCAAACA CCGCAGCATT 
CACGCCCAAC TCCTAGGACC ATCGCTGCTC CTGGCGAGTG TAGTGCAGAT GAGTGAAGAC 
CATGAACCTG AGGATAAGAG TGCAGAAGAA TACTCCTCTC TCTCTGATAC TGACTGTAGC 
CAAGATGAGC ACGAGCAAAC AGGCATGGAC TTTGAAACAG AGTCTGATAA GTGTCTGACA 
TGGCTGGCCC AGGGTTTGTT GGTGGGAGAA AGTATTCAGC CCCGGCCTCT TATCCGTGCA 
CCTCGGTGTC TGAGGCGAAA CTCCTCACAC CTTCTCCCCG CTGAGGCCGT TTACAGAAGC 
CCAGCTTCTT ATGGGCTGTA TGGCCGTTAC CGTAGGACCA GTCAGGTGCA TAATATGGGT 
GGATTGTGGG CAGGGAAGAG AAGTTCTCTC GCAGCTCGTG GATCAGTTGC ACTGTACAGG 
ACGAGCCCCC CTTGCTGGCC AGATCTCAGT GTGTCGCACA CACTTCAGGG AACTTACTAC 
AACCCACAGA TTGACACATG GAGTGGGTTC CTCTCGAAGG CTATTGCCAA GTCCGGTCTC 
CAGCACCTCT CATGCCAGCC CAGTGCTGCA GTGTTAGCCA AAGGGGAAAC GGACAGAGAA 
ATACGCAACA CAGTAGACTG GAGTGAATCA GCTGTATACG GAGAGCACAT CTGGTTTGAG 
ACGAATGTCT CGGGGGACTT CTGTTACGTT GGAGAACAGC ACTGTTACGC CAAGTCACTG 
CAAAAGTCTG TTGCTCGGAA AAAGTGTGCG GCTTGCAAGA TCGTAGTTCA CTCTATCTGC 
ATTGAACAGC TGGAGAAGAT AAACTTCAGA TGTAAACCGT CTTTCAGGGA GTCAGGATCA 
CGGAACATCC GAGAGCCTGC AATAGTGCGA CACCACTGGG TCCATCGGAG AAGACAAGAG 
GGCAAATGCA AACAGTGTGG AAAGGGGTTT CAGCAGAAAT TTGCCTTTCA CAGTAAAGAG 
ATTGTAGCCA TCAGCTGCTC CTGGTGCAAA CAAGCATATC ATAATAAGGT GACATGCTTT 
ATGCTGCAGC AGATAGAAGA ACCATGTTCA TTAGGAGCTC ATGCCGCTGT AATAGTACCT 
CCTACTTGGA TCATACGGGT TCGCCGACCT CAGTCCTCAC TAAAGTCAAG CAAAAAGAAA 
AAACGGACAT CTTTCAAACG TAAATCCAGC AAGAAAGGAG CAGAGGAGGC ACGATGGAAG 
CCTTTTATAG TGAGACCCAT TCCCTCCCAA CTCATGAAGC CACTGCTAGT GTTTGTAAAT 
CCAAAGAGTG GAGGAAACCA GGGAGCTAAA ATCATCCAGA CCTTTCTTTG GTATCTAAAC 
CCTCGGCAGG TGTTTGATCT AAGTCAAGGA GGTCCTCAGG AAGGCTTGGA AATGTATCGC 
AAGGTTCACA ACTTGAGAAT TTTGGCCTGT GGGGGGGATG GCACGGTGGG CTGGATCTTA 
TCTGCACTTG ATCAGCTGCA ATTAAACCCT TCTCCTGCTG TGGCCGTACT TCCCCTGGGC 
ACAGGAAATG ACCTTGCTAG GACGCTAAAC TGGGGTGGGG GCTACACAGA TGAACCTCTG 
AGTAAAATCC TGTCTCATGT GGAGGATGGG AATATTGTAC AGCTGGATCG GTGGAATCTT 
GTTGTGAAGC CCAACCCAGA GGCAGGACCA GAGGAAAGAG ACGAGCAGGT GACGGACAAG 
CTCCCTCTAG ACGTCTTCAA TAACTACTTC AGCCTTGGAT TTGATGCTCA TGTGACTTTA 
GAGTTCCACG AATCTCGAGA GGCTAATCCT GAGAAATTTA ACAGCCGGTT CAGAAACAAA 
ATGTTCTATG CAGGGACAGC GTTTTCTGAT TTCTTGATGG GCAGCTCCAA AGACCTGGCT 
AAGCACATCA AAGTAGTCTG TGACGGCACA GATTTAACCT CAAAGGTCCA GGATTTGAAG 
CTGCAATGCC TGGTCTTCCT AAATATCCCC AGGTACTGTG CGGGCACAAT GCCATGGGGC 
AATCCGAGTG AGCATCATGA CTTTGAGCCT CAGCGTCATG ATGATGGCTG CATTGAGGTT 
ATTGGATTCA CAATGACATC ACTGGCCACA CTGCAGGTCG GAGGTCACGG TGAGAGGTTA 
AATCAGTGTC GCGAAGTCAC CCTCACCACC TTTAAGTCAA TCCCCATGCA GGTGGACGGG 
GAGCCTTGTA AACTAGCACC ATCTGTTATT CACATTTCTT TGCGAAACCA GGCCAACATG 
GTGCAGAAAA CCAAGAGACG TACATCTATA CCACTGCTAA ACGATCAGCA ACCGTTTCCA 
GAACGACTAC GGATCAGAGT GAGCCGTATC AGCATGCATG ATTATGAAGC TTTGCACTAC 
GACAAGGAGA AGCTCAAAGA AGCTTCGATT CCACTGGGTC TCATCGTTGT CCCTGGAGAC 
AGTGATTTGG AAACCTGCCG AGCTCACATC GAGAGACTAC AGGAGGACTT TGTGTCGTGT 
AACCCGTCTC TGCAGCGGCT GGTGCTCCAG GAAGGTGACG GGAGCAAATC AAAGGCTTTG 
TCGTCTCAGA GGCTATCACC GAAATGGTGC TTTCTCGACT CTACAACTGC TGATCGATTC 
TACAGGATTG ATCGAGCTCA AGAGCATCTC AACTACGTGA CTGAAATTTC TCAGGATGAG 
CTGTTTGTTT TGGACCCAGA GCTGGTGGTC ACAGAGACTG TTAGCACATC ACCTGGCATG 
CCAGACCTGG TGGACTCTGG TGAAGTCCAG TCCGATCCAC ACAAATTTGC CTTTCCGTCA 
TCCTCTTCTT CACCACCAAA CACACCTGGA CCTAGGGTTG CAGAGCTGCA AAGGAAGCGT 
GTTTCCAGTG ACAGTTCGGT GGCAGAAGCA CTTGCACACA GTGACTCGCT CAGATCAGCC 
AAGCCAGCTC TCTGCAGAGC TGGGGGTGTT CATCGCTCAA ATACAACCGC AGCAGATTTC 
AAACCTACCA GCAGGTTTGA GAAGAGCGCT GTGAACAATG CCAACTCTGT GTCAGTGGAC 
AGTCTAATTG AATGTGTGAA AAAGAAGGAC CATCAGAAGT TGAAGGAGCT GCATAAGAAA 
GGGGCCGACC TGTGTGTGCA GGACCCTGCT GGACGGACCC TATTGCACTA TGCTGTGGAA 
GTGGGCAGCA AGGAGATTGT CAAATACATC ATTGATAATG CACCCACCGA TATCCTGGAT 
GTAACAGAGA AAGAAAATGG AGAGACTGTG TTGCACAAAG CAGCCTCGCT GTGTCATAGG 
ACAATCTGTC ATTACCTAGT GGAAGCAGGA GCATCTCTTA TGAAGACAGA CTTACAGGGT 
GACACTCCGA AGCATCGTGC CGAGAAGGCG AAAGATGCTG AACTTGCGGC ATATCTTGAA 
AACCGCCAGC ACTACCAAAT GATCCAGCGT GAGGACCAGG AGACTGCAGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	dgkza
Entrez Gene ID	553380
Full Name	diacylglycerol kinase, zeta a
Synonyms	dgki
General protein information	Preferred Names diacylglycerol kinase, zeta a Names diacylglycerol kinase zeta
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Pan troglodytes (chimpanzee)	DGKZ	XP_508395.2
Mus musculus (house mouse)	Dgkz	NP_001160069.1
Gallus gallus (chicken)	DGKZ	NP_001026363.1
Homo sapiens (human)	DGKZ	NP_001099010.1
	DGKZ	XP_001102461.2
Xenopus tropicalis (tropical clawed frog)	dgkz	XP_004913437.1
Canis lupus familiaris (dog)	DGKZ	XP_850588.2
Rattus norvegicus (Norway rat)	Dgkz	NP_112405.1
Drosophila melanogaster (fruit fly)	rdgA	NP_001259366.1
Caenorhabditis elegans (roundworm)	dgk-5	NP_495301.1
Bos taurus (cattle)	DGKZ	NP_001265581.1
Danio rerio (zebrafish)	dgkza	XP_005169089.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000519	XP_310575.5

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

dgkza cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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