npc1 cDNA ORF clone, Danio rerio(zebrafish)

The following npc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the npc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa31915 NM_001243875.1
Latest version!
Danio rerio Niemann-Pick disease, type C1 (npc1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa06625 XM_005163658.4
Latest version!
Danio rerio Niemann-Pick disease, type C1 (npc1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa06626 XM_005163659.4
Latest version!
Danio rerio Niemann-Pick disease, type C1 (npc1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
ODa06625 XM_005163658.2 Danio rerio Niemann-Pick disease, type C1 (npc1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa06626 XM_005163659.3 Danio rerio Niemann-Pick disease, type C1 (npc1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa06625 XM_005163658.3 Danio rerio Niemann-Pick disease, type C1 (npc1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa31915
    Clone ID Related Accession (Same CDS sequence) NM_001243875.1
    Accession Version NM_001243875.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3816bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-09
    Organism Danio rerio(zebrafish)
    Product Niemann-Pick C1 protein precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FP245481.6 and FP236166.8. On Sep 2, 2011 this sequence version replaced XM_001919923.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCTTCTGC TGGGGAGAAA CCACATTTTC CGTCTTTTAT TGGCCACAGT TTTATTATCA 
    CATTGGGTGC ATGGCCAGCA CTGTATCTGG TACGGCGAAT GTGGGAACTC CCCAAACGTC
    CCAGAAAAGA AGTTGAACTG CAATTACACC GGTCCTGCTG TGCCTCTGCC TAATGAAGGA
    CAGGAGCTGC TGCAGGAGCT GTGTCCGAGA CTGGTCTACG CAGACAACAG AGTTTGCTGC
    GATACACAAC AGCTCAACAC ACTAAAAAGC AACATCCAGA TCCCCCTTCA GTACCTGTCC
    AGGTGTCCAG CATGTTTCTT CAACTTCATG ACCCTGTTCT GCGAGCTCAC CTGCAGCCCC
    AGACAGAGCC AGTTTATCAG CGTAAAAGAC TTCCTTAAAG AGAAAAACCA GACCAGTGTG
    GGGAATGTGA CCTATTATAT CACCCAGACC TTTGCCGATG CCATGTTTAA CGCCTGCAGA
    GATGTCCAGG CTCCATCCAG CAACATCAAA GCTCTCGGTC TGTTGTGCGG CAGAGACGCC
    AGTGTGTGTA CTCCTCAAAT TTGGATCCAG TACATGTTTA GTATCAGCAA CGGACAGGTT
    CCCTTTGGCA TCGAACCGAT TTTCACAGAT GTCCCTGTGC AGGGCATGAC CCCAATGAAT
    AACAGGACGT TTAACTGCTC TCAGTCTCTG GATGACGGCT CGGAACCCTG CTCGTGTCAG
    GACTGCAGTG AAGTGTGCGG GCCCACGCCT GTGCCCCCTC CCATCCCTCC TCCCTGGATT
    ATCCTCGGCC TGGATGCCAT GAGCTTCATC ATGTGGTGCT CTTACATCGC TTTCCTCCTC
    ATCTTCTTCG GAGTGGTTCT GGGAGCTTGG TGTTACAGAA GGAGCGTGGT CACATCTGAA
    TATGGCCCAA TTCTTGACAG CAATCAGCCC CACTCCTTAA ACTCTGATGA TGAGGCGTCG
    TGCTGTGAGA CTGTGGGCGA GCGTTTCGAA AACTCTCTGC GTCTGGTGTT CAGCCGCTGG
    GGCTCTCTGT GCGTCCGGCA GCCGCTCACC ATCATCTTGA GCAGTCTGGT GCTGATCTGC
    ATCTGCTCAG CCGGACTCTC CTACATGCGC ATCACCACTA ACCCTGTGGA GCTCTGGTCT
    GCTCCCAGCA GCAGAGCACG GCAGGAGAAA AACTACTTCG ACCAGCACTT CGGGCCCTTC
    TTCCGCACCG AGCAGCTCAT CATCACCACT CCATGGACCG AGGAGGGAGG TTTCTCCACC
    ATCACAGGAG ACATCATTCC CTTCTCCCCC ATTCTGAACC TATCACTGCT GCATCAGGTG
    CTGGATCTGC AGTTGGAAAT CGAGAATCTG ATCGCTGAAT ATAAAGGAGA GAATGTGACT
    CTGAAAGACA TCTGTGTTTC ACCTTTGTCT CCTTACAATG ACAACTGCAC CATCCTGAGT
    GTGTTAAACT ACTTCCAGAA CAGCCATGAA GTCCTGGATC ATGAGTTTCA AGACGAATTC
    TTCCTCTACA ATGACTACCA CACTCATCTG CTCTACTGTG CCAGTTCCCC GACCTCTCTG
    GATGACACGA GCCGTTTGCA TGACCCCTGC ATGGGCACGT TCGGAGGTCC AGTTTTCCCC
    TGGCTTGTTC TTGGAGGATA TGAGGACTCC GCCTACAACA ATGCCACCGC TCTGGTGATC
    ACTTTTCCTG TCACCAACTA CCTGAATGAC ACTGAAAAAC TAGGCAAAGC TTTGGCATGG
    GAGAAAGAGT TTATTAGGTT TATGAAGAAT TACGAGAACC CGAACCTCAC GGTGTCCTTC
    AGCTCCGAGC GCAGTATCGA GGACGAGATT GACCGCGAGA GCAACAGTGA TGTTTCCACC
    ATCGTGATCA GCTACATCAT CATGTTTGTC TACATCTCAG TGGCTTTGGG CCGCATCAAC
    AGCTGCAGGA CACTGCTGGT GGACTCTAAG ATCTCTCTGG GCATCGCTGG GATCCTGATC
    GTGTTGAGTT CAGTGGCGTG TTCGCTGGGG ATCTTCAGCT ACATTGGGAT CCCGCTCACG
    CTTATCGTCA TTGAGGTCAT TCCCTTCCTC GTACTGGCCG TAGGAGTGGA CAACATCTTT
    ATCATTGTGC AGACCTATCA GAGAGACGAG CGAATGCCAG AGGAGGAGCT TCATCAGCAG
    ATTGGCCGAA TCCTCGGAGA TGTGGCTCCC AGCATGTTCC TCTCTTCCTT CTCTGAGACC
    GTGGCCTTCT TTTTAGGTGC TCTGTCCACC ATGCCTGCGG TGAGGACGTT CTCTCTGTTC
    GCTGGTCTGG CCATCTTTAT CGACTTCCTC CTGCAGATCA GCTGCTTTGT GTCTTTATTG
    GGCCTCGACA TCAAGAGACA AGAGGCCAAT CGGATGGATA TATTGTGCTG TGTGAAGTTG
    TCTGATGGAC AAGAAGAAAA ATCTGAAGGT TGGCTCTTCC GCTTCTTCAA GAAAATATAC
    GCTCCTTTCA TCCTGAAGGA CTGGGTTCGC CCTCTGGTGG TTGCTGTGTT TGTGGGAATG
    CTGTCCTTCA GCATTGCCGT TGTTAATAAA GTGGAGATTG GACTGGAGCA GACGCTATCC
    ATGCCTGATG ACTCATATGT GCTGAATTAC TTTGGGAATC TGAGCAAGTA TCTACACACC
    GGGCCGCCGG TTTACTTCGT GGTTGAAGAT GGCCATGATT ATAAGACGTT TGAGGGCCAG
    AACGCAGTGT GTGGTGGAGT CGGCTGCAAC AACGACTCTC TGGTCCAGCA GATTTACACC
    GCTTCCCTCA TGAGCAACTA CACAAGGATC AGTAATGTAC CCTCGTCCTG GCTGGATGAC
    TACTTTGACT GGGTGAAGCC GCAGTCGACC TGCTGCAGAT ATTACAACAG CACAGGAGCT
    TTCTGTAACG CTTCAGTGGT GGACAAGAGC TGCGTTCACT GCAGACCAAT GACCTCTAGT
    GGGAAACAGA GACCGAACGG CACTGAGTTC ATGCACTTCC TCCCCATGTT TCTCTCTGAC
    AACCCCAATA TCAAATGTGG AAAAGGTGGT CATGCAGCGT ACGGCACTGC CGTGGATCTC
    AAAGACAACA ACACAGATGT GGGCGCCACA TACTTCATGA GTTACCACAC CATCCTGAAA
    AACTCCTCCG ACTTTATTAA TGCCATGAAG ATGGCCAGAG AGCTTACAGA CAACATCACA
    CAGACCCTCA GCACGCATGA CAAGAGCTAC AAAGTCTTCC CTTACAGTGT GTTTTACGTC
    TTCTACGAGC AGTATCTGAC CATCGTCGAT GACACCGCGC TGAACCTCGG CGTGTCTCTG
    TCCGCCATCT TCATTGTGAC GGCAGTTCTG CTGGGCTTTG AGCTCTGGTC GGCGGTGCTC
    GTCTGCTTCA CCATCGCTAT GATCCTCATC AACATGTTCG GGGTCATGTG GCTCTGGAGC
    ATCAGCCTCA ACGCTGTCTC ATTGGTCAAC TTGGTCATGA GTTGTGGGAT ATCAGTGGAG
    TTCTGCAGTC ATATAGTGAG GGCTTTCTCC ATCAGCACCA GGAGCTCCCG GGTGGAGCGG
    GCAGAGGAGG CGTTGGCACA TATGGGCAGC TCCGTCTTCA GTGGAATCAC CCTCACCAAG
    TTTGGCGGGA TCCTGATTCT CGCTCTGTCC AAATCGCAGA TCTTCCAGAT TTTCTACTTC
    CGCATGTATC TGGCAATCGT GCTTCTGGGA GCCGCTCACG GCCTCATCTT CCTGCCTGTC
    TTACTCAGTT ATGCAGGCCC GTCTGTAAAT AAAGCAAAGG TTCTAGCTGC GCACAACAGG
    TTTGTGGGCA CAGAGAGAGA GCGGCTCATC TACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001230804.1
    CDS42..3857
    Translation

    Target ORF information:

    RefSeq Version NM_001243875.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Niemann-Pick disease, type C1 (npc1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001243875.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCTTCTGC TGGGGAGAAA CCACATTTTC CGTCTTTTAT TGGCCACAGT TTTATTATCA 
    CATTGGGTGC ATGGCCAGCA CTGTATCTGG TACGGCGAAT GTGGGAACTC CCCAAACGTC
    CCAGAAAAGA AGTTGAACTG CAATTACACC GGTCCTGCTG TGCCTCTGCC TAATGAAGGA
    CAGGAGCTGC TGCAGGAGCT GTGTCCGAGA CTGGTCTACG CAGACAACAG AGTTTGCTGC
    GATACACAAC AGCTCAACAC ACTAAAAAGC AACATCCAGA TCCCCCTTCA GTACCTGTCC
    AGGTGTCCAG CATGTTTCTT CAACTTCATG ACCCTGTTCT GCGAGCTCAC CTGCAGCCCC
    AGACAGAGCC AGTTTATCAG CGTAAAAGAC TTCCTTAAAG AGAAAAACCA GACCAGTGTG
    GGGAATGTGA CCTATTATAT CACCCAGACC TTTGCCGATG CCATGTTTAA CGCCTGCAGA
    GATGTCCAGG CTCCATCCAG CAACATCAAA GCTCTCGGTC TGTTGTGCGG CAGAGACGCC
    AGTGTGTGTA CTCCTCAAAT TTGGATCCAG TACATGTTTA GTATCAGCAA CGGACAGGTT
    CCCTTTGGCA TCGAACCGAT TTTCACAGAT GTCCCTGTGC AGGGCATGAC CCCAATGAAT
    AACAGGACGT TTAACTGCTC TCAGTCTCTG GATGACGGCT CGGAACCCTG CTCGTGTCAG
    GACTGCAGTG AAGTGTGCGG GCCCACGCCT GTGCCCCCTC CCATCCCTCC TCCCTGGATT
    ATCCTCGGCC TGGATGCCAT GAGCTTCATC ATGTGGTGCT CTTACATCGC TTTCCTCCTC
    ATCTTCTTCG GAGTGGTTCT GGGAGCTTGG TGTTACAGAA GGAGCGTGGT CACATCTGAA
    TATGGCCCAA TTCTTGACAG CAATCAGCCC CACTCCTTAA ACTCTGATGA TGAGGCGTCG
    TGCTGTGAGA CTGTGGGCGA GCGTTTCGAA AACTCTCTGC GTCTGGTGTT CAGCCGCTGG
    GGCTCTCTGT GCGTCCGGCA GCCGCTCACC ATCATCTTGA GCAGTCTGGT GCTGATCTGC
    ATCTGCTCAG CCGGACTCTC CTACATGCGC ATCACCACTA ACCCTGTGGA GCTCTGGTCT
    GCTCCCAGCA GCAGAGCACG GCAGGAGAAA AACTACTTCG ACCAGCACTT CGGGCCCTTC
    TTCCGCACCG AGCAGCTCAT CATCACCACT CCATGGACCG AGGAGGGAGG TTTCTCCACC
    ATCACAGGAG ACATCATTCC CTTCTCCCCC ATTCTGAACC TATCACTGCT GCATCAGGTG
    CTGGATCTGC AGTTGGAAAT CGAGAATCTG ATCGCTGAAT ATAAAGGAGA GAATGTGACT
    CTGAAAGACA TCTGTGTTTC ACCTTTGTCT CCTTACAATG ACAACTGCAC CATCCTGAGT
    GTGTTAAACT ACTTCCAGAA CAGCCATGAA GTCCTGGATC ATGAGTTTCA AGACGAATTC
    TTCCTCTACA ATGACTACCA CACTCATCTG CTCTACTGTG CCAGTTCCCC GACCTCTCTG
    GATGACACGA GCCGTTTGCA TGACCCCTGC ATGGGCACGT TCGGAGGTCC AGTTTTCCCC
    TGGCTTGTTC TTGGAGGATA TGAGGACTCC GCCTACAACA ATGCCACCGC TCTGGTGATC
    ACTTTTCCTG TCACCAACTA CCTGAATGAC ACTGAAAAAC TAGGCAAAGC TTTGGCATGG
    GAGAAAGAGT TTATTAGGTT TATGAAGAAT TACGAGAACC CGAACCTCAC GGTGTCCTTC
    AGCTCCGAGC GCAGTATCGA GGACGAGATT GACCGCGAGA GCAACAGTGA TGTTTCCACC
    ATCGTGATCA GCTACATCAT CATGTTTGTC TACATCTCAG TGGCTTTGGG CCGCATCAAC
    AGCTGCAGGA CACTGCTGGT GGACTCTAAG ATCTCTCTGG GCATCGCTGG GATCCTGATC
    GTGTTGAGTT CAGTGGCGTG TTCGCTGGGG ATCTTCAGCT ACATTGGGAT CCCGCTCACG
    CTTATCGTCA TTGAGGTCAT TCCCTTCCTC GTACTGGCCG TAGGAGTGGA CAACATCTTT
    ATCATTGTGC AGACCTATCA GAGAGACGAG CGAATGCCAG AGGAGGAGCT TCATCAGCAG
    ATTGGCCGAA TCCTCGGAGA TGTGGCTCCC AGCATGTTCC TCTCTTCCTT CTCTGAGACC
    GTGGCCTTCT TTTTAGGTGC TCTGTCCACC ATGCCTGCGG TGAGGACGTT CTCTCTGTTC
    GCTGGTCTGG CCATCTTTAT CGACTTCCTC CTGCAGATCA GCTGCTTTGT GTCTTTATTG
    GGCCTCGACA TCAAGAGACA AGAGGCCAAT CGGATGGATA TATTGTGCTG TGTGAAGTTG
    TCTGATGGAC AAGAAGAAAA ATCTGAAGGT TGGCTCTTCC GCTTCTTCAA GAAAATATAC
    GCTCCTTTCA TCCTGAAGGA CTGGGTTCGC CCTCTGGTGG TTGCTGTGTT TGTGGGAATG
    CTGTCCTTCA GCATTGCCGT TGTTAATAAA GTGGAGATTG GACTGGAGCA GACGCTATCC
    ATGCCTGATG ACTCATATGT GCTGAATTAC TTTGGGAATC TGAGCAAGTA TCTACACACC
    GGGCCGCCGG TTTACTTCGT GGTTGAAGAT GGCCATGATT ATAAGACGTT TGAGGGCCAG
    AACGCAGTGT GTGGTGGAGT CGGCTGCAAC AACGACTCTC TGGTCCAGCA GATTTACACC
    GCTTCCCTCA TGAGCAACTA CACAAGGATC AGTAATGTAC CCTCGTCCTG GCTGGATGAC
    TACTTTGACT GGGTGAAGCC GCAGTCGACC TGCTGCAGAT ATTACAACAG CACAGGAGCT
    TTCTGTAACG CTTCAGTGGT GGACAAGAGC TGCGTTCACT GCAGACCAAT GACCTCTAGT
    GGGAAACAGA GACCGAACGG CACTGAGTTC ATGCACTTCC TCCCCATGTT TCTCTCTGAC
    AACCCCAATA TCAAATGTGG AAAAGGTGGT CATGCAGCGT ACGGCACTGC CGTGGATCTC
    AAAGACAACA ACACAGATGT GGGCGCCACA TACTTCATGA GTTACCACAC CATCCTGAAA
    AACTCCTCCG ACTTTATTAA TGCCATGAAG ATGGCCAGAG AGCTTACAGA CAACATCACA
    CAGACCCTCA GCACGCATGA CAAGAGCTAC AAAGTCTTCC CTTACAGTGT GTTTTACGTC
    TTCTACGAGC AGTATCTGAC CATCGTCGAT GACACCGCGC TGAACCTCGG CGTGTCTCTG
    TCCGCCATCT TCATTGTGAC GGCAGTTCTG CTGGGCTTTG AGCTCTGGTC GGCGGTGCTC
    GTCTGCTTCA CCATCGCTAT GATCCTCATC AACATGTTCG GGGTCATGTG GCTCTGGAGC
    ATCAGCCTCA ACGCTGTCTC ATTGGTCAAC TTGGTCATGA GTTGTGGGAT ATCAGTGGAG
    TTCTGCAGTC ATATAGTGAG GGCTTTCTCC ATCAGCACCA GGAGCTCCCG GGTGGAGCGG
    GCAGAGGAGG CGTTGGCACA TATGGGCAGC TCCGTCTTCA GTGGAATCAC CCTCACCAAG
    TTTGGCGGGA TCCTGATTCT CGCTCTGTCC AAATCGCAGA TCTTCCAGAT TTTCTACTTC
    CGCATGTATC TGGCAATCGT GCTTCTGGGA GCCGCTCACG GCCTCATCTT CCTGCCTGTC
    TTACTCAGTT ATGCAGGCCC GTCTGTAAAT AAAGCAAAGG TTCTAGCTGC GCACAACAGG
    TTTGTGGGCA CAGAGAGAGA GCGGCTCATC TACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa06625
    Clone ID Related Accession (Same CDS sequence) XM_005163658.2 , XM_005163658.3 , XM_005163658.4
    Accession Version XM_005163658.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3834bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-09-23
    Organism Danio rerio(Zebrafish)
    Product Niemann-Pick C1 protein isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCTTCTGC TGGGGAGAAA CCACATTTTC CGTCTTTTAT TGGCCACAGT TTTATTATCA 
    CATTGGGTGC ATGGCCAGCA CTGTATCTGG TACGGCGAAT GTGGGAACTC CCCAAACGTC
    CCAGAAAAGA AGTTGAACTG CAATTACACC GGTCCTGCTG TGCCTCTGCC TAATGAAGGA
    CAGGAGCTGC TGCAGGAGCT GTGTCCGAGA CTGGTCTACG CAGACAACAG AGTTTGCTGC
    GATACACAAC AGCTCAACAC ACTAAAAAGC AACATCCAGA TCCCCCTTCA GTACCTGTCC
    AGGTGTCCAG CATGTTTCTT CAACTTCATG ACCCTGTTCT GCGAGCTCAC CTGCAGCCCC
    AGACAGAGCC AGTTTATCAG CGTAAAAGAC TTCCTTAAAG AGAAAAACCA GACCAGTGTG
    GGGAATGTGA CCTATTATAT CACCCAGACC TTTGCCGATG CCATGTTTAA CGCCTGCAGA
    GATGTCCAGG CTCCATCCAG CAACATCAAA GCTCTCGGTC TGTTGTGCGG CAGAGACGCC
    AGTGTGTGTA CTCCTCAAAT TTGGATCCAG TACATGTTTA GTATCAGCAA CGGACAGGTT
    CCCTTTGGCA TCGAACCGAT TTTCACAGAT GTCCCTGTGC AGGGCATGAC CCCAATGAAT
    AACAGGACGT TTAACTGCTC TCAGTCTCTG GATGACGGCT CGGAACCCTG CTCGTGTCAG
    GACTGCAGTG AAGTGTGCGG GCCCACGCCT GTGCCCCCTC CCATCCCTCC TCCCTGGATT
    ATCCTCGGCC TGGATGCCAT GAGCTTCATC ATGTGGTGCT CTTACATCGC TTTCCTCCTC
    ATCTTCTTCG GAGTGGTTCT GGGAGCTTGG TGTTACAGAA GGAGCGTGGT CACATCTGAA
    TATGGCCCAA TTCTTGACAG CAATCAGCCC CACTCCTTAA ACTCTGATGG CACAGATTTA
    ATAGATGAGG CGTCGTGCTG TGAGACTGTG GGCGAGCGTT TCGAAAACTC TCTGCGTCTG
    GTGTTCAGCC GCTGGGGCTC TCTGTGCGTC CGGCAGCCGC TCACCATCAT CTTGAGCAGT
    CTGGTGCTGA TCTGCATCTG CTCAGCCGGA CTCTCCTACA TGCGCATCAC CACTAACCCT
    GTGGAGCTCT GGTCTGCTCC CAGCAGCAGA GCACGGCAGG AGAAAAACTA CTTCGACCAG
    CACTTCGGGC CCTTCTTCCG CACCGAGCAG CTCATCATCA CCACTCCATG GACCGAGGAG
    GGAGGTTTCT CCACCATCAC AGGAGACATC ATTCCCTTCT CCCCCATTCT GAACCTATCA
    CTGCTGCATC AGGTGCTGGA TCTGCAGTTG GAAATCGAGA ATCTGATCGC TGAATATAAA
    GGAGAGAATG TGACTCTGAA AGACATCTGT GTTTCACCTT TGTCTCCTTA CAATGACAAC
    TGCACCATCC TGAGTGTGTT AAACTACTTC CAGAACAGCC ATGAAGTCCT GGATCATGAG
    TTTCAAGACG AATTCTTCCT CTACAATGAC TACCACACTC ATCTGCTCTA CTGTGCCAGT
    TCCCCGACCT CTCTGGATGA CACGAGCCGT TTGCATGACC CCTGCATGGG CACGTTCGGA
    GGTCCAGTTT TCCCCTGGCT TGTTCTTGGA GGATATGAGG ACTCCGCCTA CAACAATGCC
    ACCGCTCTGG TGATCACTTT TCCTGTCACC AACTACCTGA ATGACACTGA AAAACTAGGC
    AAAGCTTTGG CATGGGAGAA AGAGTTTATT AGGTTTATGA AGAATTACGA GAACCCGAAC
    CTCACGGTGT CCTTCAGCTC CGAGCGCAGT ATCGAGGACG AGATTGACCG CGAGAGCAAC
    AGTGATGTTT CCACCATCGT GATCAGCTAC ATCATCATGT TTGTCTACAT CTCAGTGGCT
    TTGGGCCGCA TCAACAGCTG CAGGACACTG CTGGTGGACT CTAAGATCTC TCTGGGCATC
    GCTGGGATCC TGATCGTGTT GAGTTCAGTG GCGTGTTCGC TGGGGATCTT CAGCTACATT
    GGGATCCCGC TCACGCTTAT CGTCATTGAG GTCATTCCCT TCCTCGTACT GGCCGTAGGA
    GTGGACAACA TCTTTATCAT TGTGCAGACC TATCAGAGAG ACGAGCGAAT GCCAGAGGAG
    GAGCTTCATC AGCAGATTGG CCGAATCCTC GGAGATGTGG CTCCCAGCAT GTTCCTCTCT
    TCCTTCTCTG AGACCGTGGC CTTCTTTTTA GGTGCTCTGT CCACCATGCC TGCGGTGAGG
    ACGTTCTCTC TGTTCGCTGG TCTGGCCATC TTTATCGACT TCCTCCTGCA GATCAGCTGC
    TTTGTGTCTT TATTGGGCCT CGACATCAAG AGACAAGAGG CCAATCGGAT GGATATATTG
    TGCTGTGTGA AGTTGTCTGA TGGACAAGAA GAAAAATCTG AAGGTTGGCT CTTCCGCTTC
    TTCAAGAAAA TATACGCTCC TTTCATCCTG AAGGACTGGG TTCGCCCTCT GGTGGTTGCT
    GTGTTTGTGG GAATGCTGTC CTTCAGCATT GCCGTTGTTA ATAAAGTGGA GATTGGACTG
    GAGCAGACGC TATCCATGCC TGATGACTCA TATGTGCTGA ATTACTTTGG GAATCTGAGC
    AAGTATCTAC ACACCGGGCC GCCGGTTTAC TTCGTGGTTG AAGATGGCCA TGATTATAAG
    ACGTTTGAGG GCCAGAACGC AGTGTGTGGT GGAGTCGGCT GCAACAACGA CTCTCTGGTC
    CAGCAGATTT ACACCGCTTC CCTCATGAGC AACTACACAA GGATCAGTAA TGTACCCTCG
    TCCTGGCTGG ATGACTACTT TGACTGGGTG AAGCCGCAGT CGACCTGCTG CAGATATTAC
    AACAGCACAG GAGCTTTCTG TAACGCTTCA GTGGTGGACA AGAGCTGCGT TCACTGCAGA
    CCAATGACCT CTAGTGGGAA ACAGAGACCG AACGGCACTG AGTTCATGCA CTTCCTCCCC
    ATGTTTCTCT CTGACAACCC CAATATCAAA TGTGGAAAAG GTGGTCATGC AGCGTACGGC
    ACTGCCGTGG ATCTCAAAGA CAACAACACA GATGTGGGCG CCACATACTT CATGAGTTAC
    CACACCATCC TGAAAAACTC CTCCGACTTT ATTAATGCCA TGAAGATGGC CAGAGAGCTT
    ACAGACAACA TCACACAGAC CCTCAGCACG CATGACAAGA GCTACAAAGT CTTCCCTTAC
    AGTGTGTTTT ACGTCTTCTA CGAGCAGTAT CTGACCATCG TCGATGACAC CGCGCTGAAC
    CTCGGCGTGT CTCTGTCCGC CATCTTCATT GTGACGGCAG TTCTGCTGGG CTTTGAGCTC
    TGGTCGGCGG TGCTCGTCTG CTTCACCATC GCTATGATCC TCATCAACAT GTTCGGGGTC
    ATGTGGCTCT GGAGCATCAG CCTCAACGCT GTCTCATTGG TCAACTTGGT CATGAGTTGT
    GGGATATCAG TGGAGTTCTG CAGTCATATA GTGAGGGCTT TCTCCATCAG CACCAGGAGC
    TCCCGGGTGG AGCGGGCAGA GGAGGCGTTG GCACATATGG GCAGCTCCGT CTTCAGTGGA
    ATCACCCTCA CCAAGTTTGG CGGGATCCTG ATTCTCGCTC TGTCCAAATC GCAGATCTTC
    CAGATTTTCT ACTTCCGCAT GTATCTGGCA ATCGTGCTTC TGGGAGCCGC TCACGGCCTC
    ATCTTCCTGC CTGTCTTACT CAGTTATGCA GGTAAATCAC TATCTCCGAG AACATTCATG
    ATTTGTCTTG AAGCAGTAGT TCATTTAAAA CATTTAAAGA CAAGGCATCA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005163715.1
    Translation

    Target ORF information:

    RefSeq Version XM_005163658.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Niemann-Pick disease, type C1 (npc1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005163658.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCTTCTGC TGGGGAGAAA CCACATTTTC CGTCTTTTAT TGGCCACAGT TTTATTATCA 
    CATTGGGTGC ATGGCCAGCA CTGTATCTGG TACGGCGAAT GTGGGAACTC CCCAAACGTC
    CCAGAAAAGA AGTTGAACTG CAATTACACC GGTCCTGCTG TGCCTCTGCC TAATGAAGGA
    CAGGAGCTGC TGCAGGAGCT GTGTCCGAGA CTGGTCTACG CAGACAACAG AGTTTGCTGC
    GATACACAAC AGCTCAACAC ACTAAAAAGC AACATCCAGA TCCCCCTTCA GTACCTGTCC
    AGGTGTCCAG CATGTTTCTT CAACTTCATG ACCCTGTTCT GCGAGCTCAC CTGCAGCCCC
    AGACAGAGCC AGTTTATCAG CGTAAAAGAC TTCCTTAAAG AGAAAAACCA GACCAGTGTG
    GGGAATGTGA CCTATTATAT CACCCAGACC TTTGCCGATG CCATGTTTAA CGCCTGCAGA
    GATGTCCAGG CTCCATCCAG CAACATCAAA GCTCTCGGTC TGTTGTGCGG CAGAGACGCC
    AGTGTGTGTA CTCCTCAAAT TTGGATCCAG TACATGTTTA GTATCAGCAA CGGACAGGTT
    CCCTTTGGCA TCGAACCGAT TTTCACAGAT GTCCCTGTGC AGGGCATGAC CCCAATGAAT
    AACAGGACGT TTAACTGCTC TCAGTCTCTG GATGACGGCT CGGAACCCTG CTCGTGTCAG
    GACTGCAGTG AAGTGTGCGG GCCCACGCCT GTGCCCCCTC CCATCCCTCC TCCCTGGATT
    ATCCTCGGCC TGGATGCCAT GAGCTTCATC ATGTGGTGCT CTTACATCGC TTTCCTCCTC
    ATCTTCTTCG GAGTGGTTCT GGGAGCTTGG TGTTACAGAA GGAGCGTGGT CACATCTGAA
    TATGGCCCAA TTCTTGACAG CAATCAGCCC CACTCCTTAA ACTCTGATGG CACAGATTTA
    ATAGATGAGG CGTCGTGCTG TGAGACTGTG GGCGAGCGTT TCGAAAACTC TCTGCGTCTG
    GTGTTCAGCC GCTGGGGCTC TCTGTGCGTC CGGCAGCCGC TCACCATCAT CTTGAGCAGT
    CTGGTGCTGA TCTGCATCTG CTCAGCCGGA CTCTCCTACA TGCGCATCAC CACTAACCCT
    GTGGAGCTCT GGTCTGCTCC CAGCAGCAGA GCACGGCAGG AGAAAAACTA CTTCGACCAG
    CACTTCGGGC CCTTCTTCCG CACCGAGCAG CTCATCATCA CCACTCCATG GACCGAGGAG
    GGAGGTTTCT CCACCATCAC AGGAGACATC ATTCCCTTCT CCCCCATTCT GAACCTATCA
    CTGCTGCATC AGGTGCTGGA TCTGCAGTTG GAAATCGAGA ATCTGATCGC TGAATATAAA
    GGAGAGAATG TGACTCTGAA AGACATCTGT GTTTCACCTT TGTCTCCTTA CAATGACAAC
    TGCACCATCC TGAGTGTGTT AAACTACTTC CAGAACAGCC ATGAAGTCCT GGATCATGAG
    TTTCAAGACG AATTCTTCCT CTACAATGAC TACCACACTC ATCTGCTCTA CTGTGCCAGT
    TCCCCGACCT CTCTGGATGA CACGAGCCGT TTGCATGACC CCTGCATGGG CACGTTCGGA
    GGTCCAGTTT TCCCCTGGCT TGTTCTTGGA GGATATGAGG ACTCCGCCTA CAACAATGCC
    ACCGCTCTGG TGATCACTTT TCCTGTCACC AACTACCTGA ATGACACTGA AAAACTAGGC
    AAAGCTTTGG CATGGGAGAA AGAGTTTATT AGGTTTATGA AGAATTACGA GAACCCGAAC
    CTCACGGTGT CCTTCAGCTC CGAGCGCAGT ATCGAGGACG AGATTGACCG CGAGAGCAAC
    AGTGATGTTT CCACCATCGT GATCAGCTAC ATCATCATGT TTGTCTACAT CTCAGTGGCT
    TTGGGCCGCA TCAACAGCTG CAGGACACTG CTGGTGGACT CTAAGATCTC TCTGGGCATC
    GCTGGGATCC TGATCGTGTT GAGTTCAGTG GCGTGTTCGC TGGGGATCTT CAGCTACATT
    GGGATCCCGC TCACGCTTAT CGTCATTGAG GTCATTCCCT TCCTCGTACT GGCCGTAGGA
    GTGGACAACA TCTTTATCAT TGTGCAGACC TATCAGAGAG ACGAGCGAAT GCCAGAGGAG
    GAGCTTCATC AGCAGATTGG CCGAATCCTC GGAGATGTGG CTCCCAGCAT GTTCCTCTCT
    TCCTTCTCTG AGACCGTGGC CTTCTTTTTA GGTGCTCTGT CCACCATGCC TGCGGTGAGG
    ACGTTCTCTC TGTTCGCTGG TCTGGCCATC TTTATCGACT TCCTCCTGCA GATCAGCTGC
    TTTGTGTCTT TATTGGGCCT CGACATCAAG AGACAAGAGG CCAATCGGAT GGATATATTG
    TGCTGTGTGA AGTTGTCTGA TGGACAAGAA GAAAAATCTG AAGGTTGGCT CTTCCGCTTC
    TTCAAGAAAA TATACGCTCC TTTCATCCTG AAGGACTGGG TTCGCCCTCT GGTGGTTGCT
    GTGTTTGTGG GAATGCTGTC CTTCAGCATT GCCGTTGTTA ATAAAGTGGA GATTGGACTG
    GAGCAGACGC TATCCATGCC TGATGACTCA TATGTGCTGA ATTACTTTGG GAATCTGAGC
    AAGTATCTAC ACACCGGGCC GCCGGTTTAC TTCGTGGTTG AAGATGGCCA TGATTATAAG
    ACGTTTGAGG GCCAGAACGC AGTGTGTGGT GGAGTCGGCT GCAACAACGA CTCTCTGGTC
    CAGCAGATTT ACACCGCTTC CCTCATGAGC AACTACACAA GGATCAGTAA TGTACCCTCG
    TCCTGGCTGG ATGACTACTT TGACTGGGTG AAGCCGCAGT CGACCTGCTG CAGATATTAC
    AACAGCACAG GAGCTTTCTG TAACGCTTCA GTGGTGGACA AGAGCTGCGT TCACTGCAGA
    CCAATGACCT CTAGTGGGAA ACAGAGACCG AACGGCACTG AGTTCATGCA CTTCCTCCCC
    ATGTTTCTCT CTGACAACCC CAATATCAAA TGTGGAAAAG GTGGTCATGC AGCGTACGGC
    ACTGCCGTGG ATCTCAAAGA CAACAACACA GATGTGGGCG CCACATACTT CATGAGTTAC
    CACACCATCC TGAAAAACTC CTCCGACTTT ATTAATGCCA TGAAGATGGC CAGAGAGCTT
    ACAGACAACA TCACACAGAC CCTCAGCACG CATGACAAGA GCTACAAAGT CTTCCCTTAC
    AGTGTGTTTT ACGTCTTCTA CGAGCAGTAT CTGACCATCG TCGATGACAC CGCGCTGAAC
    CTCGGCGTGT CTCTGTCCGC CATCTTCATT GTGACGGCAG TTCTGCTGGG CTTTGAGCTC
    TGGTCGGCGG TGCTCGTCTG CTTCACCATC GCTATGATCC TCATCAACAT GTTCGGGGTC
    ATGTGGCTCT GGAGCATCAG CCTCAACGCT GTCTCATTGG TCAACTTGGT CATGAGTTGT
    GGGATATCAG TGGAGTTCTG CAGTCATATA GTGAGGGCTT TCTCCATCAG CACCAGGAGC
    TCCCGGGTGG AGCGGGCAGA GGAGGCGTTG GCACATATGG GCAGCTCCGT CTTCAGTGGA
    ATCACCCTCA CCAAGTTTGG CGGGATCCTG ATTCTCGCTC TGTCCAAATC GCAGATCTTC
    CAGATTTTCT ACTTCCGCAT GTATCTGGCA ATCGTGCTTC TGGGAGCCGC TCACGGCCTC
    ATCTTCCTGC CTGTCTTACT CAGTTATGCA GGTAAATCAC TATCTCCGAG AACATTCATG
    ATTTGTCTTG AAGCAGTAGT TCATTTAAAA CATTTAAAGA CAAGGCATCA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa06625
    Clone ID Related Accession (Same CDS sequence) XM_005163658.2 , XM_005163658.3 , XM_005163658.4
    Accession Version XM_005163658.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3834bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product Niemann-Pick C1 protein isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005163658.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCTTCTGC TGGGGAGAAA CCACATTTTC CGTCTTTTAT TGGCCACAGT TTTATTATCA 
    CATTGGGTGC ATGGCCAGCA CTGTATCTGG TACGGCGAAT GTGGGAACTC CCCAAACGTC
    CCAGAAAAGA AGTTGAACTG CAATTACACC GGTCCTGCTG TGCCTCTGCC TAATGAAGGA
    CAGGAGCTGC TGCAGGAGCT GTGTCCGAGA CTGGTCTACG CAGACAACAG AGTTTGCTGC
    GATACACAAC AGCTCAACAC ACTAAAAAGC AACATCCAGA TCCCCCTTCA GTACCTGTCC
    AGGTGTCCAG CATGTTTCTT CAACTTCATG ACCCTGTTCT GCGAGCTCAC CTGCAGCCCC
    AGACAGAGCC AGTTTATCAG CGTAAAAGAC TTCCTTAAAG AGAAAAACCA GACCAGTGTG
    GGGAATGTGA CCTATTATAT CACCCAGACC TTTGCCGATG CCATGTTTAA CGCCTGCAGA
    GATGTCCAGG CTCCATCCAG CAACATCAAA GCTCTCGGTC TGTTGTGCGG CAGAGACGCC
    AGTGTGTGTA CTCCTCAAAT TTGGATCCAG TACATGTTTA GTATCAGCAA CGGACAGGTT
    CCCTTTGGCA TCGAACCGAT TTTCACAGAT GTCCCTGTGC AGGGCATGAC CCCAATGAAT
    AACAGGACGT TTAACTGCTC TCAGTCTCTG GATGACGGCT CGGAACCCTG CTCGTGTCAG
    GACTGCAGTG AAGTGTGCGG GCCCACGCCT GTGCCCCCTC CCATCCCTCC TCCCTGGATT
    ATCCTCGGCC TGGATGCCAT GAGCTTCATC ATGTGGTGCT CTTACATCGC TTTCCTCCTC
    ATCTTCTTCG GAGTGGTTCT GGGAGCTTGG TGTTACAGAA GGAGCGTGGT CACATCTGAA
    TATGGCCCAA TTCTTGACAG CAATCAGCCC CACTCCTTAA ACTCTGATGG CACAGATTTA
    ATAGATGAGG CGTCGTGCTG TGAGACTGTG GGCGAGCGTT TCGAAAACTC TCTGCGTCTG
    GTGTTCAGCC GCTGGGGCTC TCTGTGCGTC CGGCAGCCGC TCACCATCAT CTTGAGCAGT
    CTGGTGCTGA TCTGCATCTG CTCAGCCGGA CTCTCCTACA TGCGCATCAC CACTAACCCT
    GTGGAGCTCT GGTCTGCTCC CAGCAGCAGA GCACGGCAGG AGAAAAACTA CTTCGACCAG
    CACTTCGGGC CCTTCTTCCG CACCGAGCAG CTCATCATCA CCACTCCATG GACCGAGGAG
    GGAGGTTTCT CCACCATCAC AGGAGACATC ATTCCCTTCT CCCCCATTCT GAACCTATCA
    CTGCTGCATC AGGTGCTGGA TCTGCAGTTG GAAATCGAGA ATCTGATCGC TGAATATAAA
    GGAGAGAATG TGACTCTGAA AGACATCTGT GTTTCACCTT TGTCTCCTTA CAATGACAAC
    TGCACCATCC TGAGTGTGTT AAACTACTTC CAGAACAGCC ATGAAGTCCT GGATCATGAG
    TTTCAAGACG AATTCTTCCT CTACAATGAC TACCACACTC ATCTGCTCTA CTGTGCCAGT
    TCCCCGACCT CTCTGGATGA CACGAGCCGT TTGCATGACC CCTGCATGGG CACGTTCGGA
    GGTCCAGTTT TCCCCTGGCT TGTTCTTGGA GGATATGAGG ACTCCGCCTA CAACAATGCC
    ACCGCTCTGG TGATCACTTT TCCTGTCACC AACTACCTGA ATGACACTGA AAAACTAGGC
    AAAGCTTTGG CATGGGAGAA AGAGTTTATT AGGTTTATGA AGAATTACGA GAACCCGAAC
    CTCACGGTGT CCTTCAGCTC CGAGCGCAGT ATCGAGGACG AGATTGACCG CGAGAGCAAC
    AGTGATGTTT CCACCATCGT GATCAGCTAC ATCATCATGT TTGTCTACAT CTCAGTGGCT
    TTGGGCCGCA TCAACAGCTG CAGGACACTG CTGGTGGACT CTAAGATCTC TCTGGGCATC
    GCTGGGATCC TGATCGTGTT GAGTTCAGTG GCGTGTTCGC TGGGGATCTT CAGCTACATT
    GGGATCCCGC TCACGCTTAT CGTCATTGAG GTCATTCCCT TCCTCGTACT GGCCGTAGGA
    GTGGACAACA TCTTTATCAT TGTGCAGACC TATCAGAGAG ACGAGCGAAT GCCAGAGGAG
    GAGCTTCATC AGCAGATTGG CCGAATCCTC GGAGATGTGG CTCCCAGCAT GTTCCTCTCT
    TCCTTCTCTG AGACCGTGGC CTTCTTTTTA GGTGCTCTGT CCACCATGCC TGCGGTGAGG
    ACGTTCTCTC TGTTCGCTGG TCTGGCCATC TTTATCGACT TCCTCCTGCA GATCAGCTGC
    TTTGTGTCTT TATTGGGCCT CGACATCAAG AGACAAGAGG CCAATCGGAT GGATATATTG
    TGCTGTGTGA AGTTGTCTGA TGGACAAGAA GAAAAATCTG AAGGTTGGCT CTTCCGCTTC
    TTCAAGAAAA TATACGCTCC TTTCATCCTG AAGGACTGGG TTCGCCCTCT GGTGGTTGCT
    GTGTTTGTGG GAATGCTGTC CTTCAGCATT GCCGTTGTTA ATAAAGTGGA GATTGGACTG
    GAGCAGACGC TATCCATGCC TGATGACTCA TATGTGCTGA ATTACTTTGG GAATCTGAGC
    AAGTATCTAC ACACCGGGCC GCCGGTTTAC TTCGTGGTTG AAGATGGCCA TGATTATAAG
    ACGTTTGAGG GCCAGAACGC AGTGTGTGGT GGAGTCGGCT GCAACAACGA CTCTCTGGTC
    CAGCAGATTT ACACCGCTTC CCTCATGAGC AACTACACAA GGATCAGTAA TGTACCCTCG
    TCCTGGCTGG ATGACTACTT TGACTGGGTG AAGCCGCAGT CGACCTGCTG CAGATATTAC
    AACAGCACAG GAGCTTTCTG TAACGCTTCA GTGGTGGACA AGAGCTGCGT TCACTGCAGA
    CCAATGACCT CTAGTGGGAA ACAGAGACCG AACGGCACTG AGTTCATGCA CTTCCTCCCC
    ATGTTTCTCT CTGACAACCC CAATATCAAA TGTGGAAAAG GTGGTCATGC AGCGTACGGC
    ACTGCCGTGG ATCTCAAAGA CAACAACACA GATGTGGGCG CCACATACTT CATGAGTTAC
    CACACCATCC TGAAAAACTC CTCCGACTTT ATTAATGCCA TGAAGATGGC CAGAGAGCTT
    ACAGACAACA TCACACAGAC CCTCAGCACG CATGACAAGA GCTACAAAGT CTTCCCTTAC
    AGTGTGTTTT ACGTCTTCTA CGAGCAGTAT CTGACCATCG TCGATGACAC CGCGCTGAAC
    CTCGGCGTGT CTCTGTCCGC CATCTTCATT GTGACGGCAG TTCTGCTGGG CTTTGAGCTC
    TGGTCGGCGG TGCTCGTCTG CTTCACCATC GCTATGATCC TCATCAACAT GTTCGGGGTC
    ATGTGGCTCT GGAGCATCAG CCTCAACGCT GTCTCATTGG TCAACTTGGT CATGAGTTGT
    GGGATATCAG TGGAGTTCTG CAGTCATATA GTGAGGGCTT TCTCCATCAG CACCAGGAGC
    TCCCGGGTGG AGCGGGCAGA GGAGGCGTTG GCACATATGG GCAGCTCCGT CTTCAGTGGA
    ATCACCCTCA CCAAGTTTGG CGGGATCCTG ATTCTCGCTC TGTCCAAATC GCAGATCTTC
    CAGATTTTCT ACTTCCGCAT GTATCTGGCA ATCGTGCTTC TGGGAGCCGC TCACGGCCTC
    ATCTTCCTGC CTGTCTTACT CAGTTATGCA GGTAAATCAC TATCTCCGAG AACATTCATG
    ATTTGTCTTG AAGCAGTAGT TCATTTAAAA CATTTAAAGA CAAGGCATCA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005163715.1
    CDS1..3834
    Translation

    Target ORF information:

    RefSeq Version XM_005163658.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Niemann-Pick disease, type C1 (npc1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005163658.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCTTCTGC TGGGGAGAAA CCACATTTTC CGTCTTTTAT TGGCCACAGT TTTATTATCA 
    CATTGGGTGC ATGGCCAGCA CTGTATCTGG TACGGCGAAT GTGGGAACTC CCCAAACGTC
    CCAGAAAAGA AGTTGAACTG CAATTACACC GGTCCTGCTG TGCCTCTGCC TAATGAAGGA
    CAGGAGCTGC TGCAGGAGCT GTGTCCGAGA CTGGTCTACG CAGACAACAG AGTTTGCTGC
    GATACACAAC AGCTCAACAC ACTAAAAAGC AACATCCAGA TCCCCCTTCA GTACCTGTCC
    AGGTGTCCAG CATGTTTCTT CAACTTCATG ACCCTGTTCT GCGAGCTCAC CTGCAGCCCC
    AGACAGAGCC AGTTTATCAG CGTAAAAGAC TTCCTTAAAG AGAAAAACCA GACCAGTGTG
    GGGAATGTGA CCTATTATAT CACCCAGACC TTTGCCGATG CCATGTTTAA CGCCTGCAGA
    GATGTCCAGG CTCCATCCAG CAACATCAAA GCTCTCGGTC TGTTGTGCGG CAGAGACGCC
    AGTGTGTGTA CTCCTCAAAT TTGGATCCAG TACATGTTTA GTATCAGCAA CGGACAGGTT
    CCCTTTGGCA TCGAACCGAT TTTCACAGAT GTCCCTGTGC AGGGCATGAC CCCAATGAAT
    AACAGGACGT TTAACTGCTC TCAGTCTCTG GATGACGGCT CGGAACCCTG CTCGTGTCAG
    GACTGCAGTG AAGTGTGCGG GCCCACGCCT GTGCCCCCTC CCATCCCTCC TCCCTGGATT
    ATCCTCGGCC TGGATGCCAT GAGCTTCATC ATGTGGTGCT CTTACATCGC TTTCCTCCTC
    ATCTTCTTCG GAGTGGTTCT GGGAGCTTGG TGTTACAGAA GGAGCGTGGT CACATCTGAA
    TATGGCCCAA TTCTTGACAG CAATCAGCCC CACTCCTTAA ACTCTGATGG CACAGATTTA
    ATAGATGAGG CGTCGTGCTG TGAGACTGTG GGCGAGCGTT TCGAAAACTC TCTGCGTCTG
    GTGTTCAGCC GCTGGGGCTC TCTGTGCGTC CGGCAGCCGC TCACCATCAT CTTGAGCAGT
    CTGGTGCTGA TCTGCATCTG CTCAGCCGGA CTCTCCTACA TGCGCATCAC CACTAACCCT
    GTGGAGCTCT GGTCTGCTCC CAGCAGCAGA GCACGGCAGG AGAAAAACTA CTTCGACCAG
    CACTTCGGGC CCTTCTTCCG CACCGAGCAG CTCATCATCA CCACTCCATG GACCGAGGAG
    GGAGGTTTCT CCACCATCAC AGGAGACATC ATTCCCTTCT CCCCCATTCT GAACCTATCA
    CTGCTGCATC AGGTGCTGGA TCTGCAGTTG GAAATCGAGA ATCTGATCGC TGAATATAAA
    GGAGAGAATG TGACTCTGAA AGACATCTGT GTTTCACCTT TGTCTCCTTA CAATGACAAC
    TGCACCATCC TGAGTGTGTT AAACTACTTC CAGAACAGCC ATGAAGTCCT GGATCATGAG
    TTTCAAGACG AATTCTTCCT CTACAATGAC TACCACACTC ATCTGCTCTA CTGTGCCAGT
    TCCCCGACCT CTCTGGATGA CACGAGCCGT TTGCATGACC CCTGCATGGG CACGTTCGGA
    GGTCCAGTTT TCCCCTGGCT TGTTCTTGGA GGATATGAGG ACTCCGCCTA CAACAATGCC
    ACCGCTCTGG TGATCACTTT TCCTGTCACC AACTACCTGA ATGACACTGA AAAACTAGGC
    AAAGCTTTGG CATGGGAGAA AGAGTTTATT AGGTTTATGA AGAATTACGA GAACCCGAAC
    CTCACGGTGT CCTTCAGCTC CGAGCGCAGT ATCGAGGACG AGATTGACCG CGAGAGCAAC
    AGTGATGTTT CCACCATCGT GATCAGCTAC ATCATCATGT TTGTCTACAT CTCAGTGGCT
    TTGGGCCGCA TCAACAGCTG CAGGACACTG CTGGTGGACT CTAAGATCTC TCTGGGCATC
    GCTGGGATCC TGATCGTGTT GAGTTCAGTG GCGTGTTCGC TGGGGATCTT CAGCTACATT
    GGGATCCCGC TCACGCTTAT CGTCATTGAG GTCATTCCCT TCCTCGTACT GGCCGTAGGA
    GTGGACAACA TCTTTATCAT TGTGCAGACC TATCAGAGAG ACGAGCGAAT GCCAGAGGAG
    GAGCTTCATC AGCAGATTGG CCGAATCCTC GGAGATGTGG CTCCCAGCAT GTTCCTCTCT
    TCCTTCTCTG AGACCGTGGC CTTCTTTTTA GGTGCTCTGT CCACCATGCC TGCGGTGAGG
    ACGTTCTCTC TGTTCGCTGG TCTGGCCATC TTTATCGACT TCCTCCTGCA GATCAGCTGC
    TTTGTGTCTT TATTGGGCCT CGACATCAAG AGACAAGAGG CCAATCGGAT GGATATATTG
    TGCTGTGTGA AGTTGTCTGA TGGACAAGAA GAAAAATCTG AAGGTTGGCT CTTCCGCTTC
    TTCAAGAAAA TATACGCTCC TTTCATCCTG AAGGACTGGG TTCGCCCTCT GGTGGTTGCT
    GTGTTTGTGG GAATGCTGTC CTTCAGCATT GCCGTTGTTA ATAAAGTGGA GATTGGACTG
    GAGCAGACGC TATCCATGCC TGATGACTCA TATGTGCTGA ATTACTTTGG GAATCTGAGC
    AAGTATCTAC ACACCGGGCC GCCGGTTTAC TTCGTGGTTG AAGATGGCCA TGATTATAAG
    ACGTTTGAGG GCCAGAACGC AGTGTGTGGT GGAGTCGGCT GCAACAACGA CTCTCTGGTC
    CAGCAGATTT ACACCGCTTC CCTCATGAGC AACTACACAA GGATCAGTAA TGTACCCTCG
    TCCTGGCTGG ATGACTACTT TGACTGGGTG AAGCCGCAGT CGACCTGCTG CAGATATTAC
    AACAGCACAG GAGCTTTCTG TAACGCTTCA GTGGTGGACA AGAGCTGCGT TCACTGCAGA
    CCAATGACCT CTAGTGGGAA ACAGAGACCG AACGGCACTG AGTTCATGCA CTTCCTCCCC
    ATGTTTCTCT CTGACAACCC CAATATCAAA TGTGGAAAAG GTGGTCATGC AGCGTACGGC
    ACTGCCGTGG ATCTCAAAGA CAACAACACA GATGTGGGCG CCACATACTT CATGAGTTAC
    CACACCATCC TGAAAAACTC CTCCGACTTT ATTAATGCCA TGAAGATGGC CAGAGAGCTT
    ACAGACAACA TCACACAGAC CCTCAGCACG CATGACAAGA GCTACAAAGT CTTCCCTTAC
    AGTGTGTTTT ACGTCTTCTA CGAGCAGTAT CTGACCATCG TCGATGACAC CGCGCTGAAC
    CTCGGCGTGT CTCTGTCCGC CATCTTCATT GTGACGGCAG TTCTGCTGGG CTTTGAGCTC
    TGGTCGGCGG TGCTCGTCTG CTTCACCATC GCTATGATCC TCATCAACAT GTTCGGGGTC
    ATGTGGCTCT GGAGCATCAG CCTCAACGCT GTCTCATTGG TCAACTTGGT CATGAGTTGT
    GGGATATCAG TGGAGTTCTG CAGTCATATA GTGAGGGCTT TCTCCATCAG CACCAGGAGC
    TCCCGGGTGG AGCGGGCAGA GGAGGCGTTG GCACATATGG GCAGCTCCGT CTTCAGTGGA
    ATCACCCTCA CCAAGTTTGG CGGGATCCTG ATTCTCGCTC TGTCCAAATC GCAGATCTTC
    CAGATTTTCT ACTTCCGCAT GTATCTGGCA ATCGTGCTTC TGGGAGCCGC TCACGGCCTC
    ATCTTCCTGC CTGTCTTACT CAGTTATGCA GGTAAATCAC TATCTCCGAG AACATTCATG
    ATTTGTCTTG AAGCAGTAGT TCATTTAAAA CATTTAAAGA CAAGGCATCA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa06625
    Clone ID Related Accession (Same CDS sequence) XM_005163658.2 , XM_005163658.3 , XM_005163658.4
    Accession Version XM_005163658.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3834bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product Niemann-Pick C1 protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005163658.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCTTCTGC TGGGGAGAAA CCACATTTTC CGTCTTTTAT TGGCCACAGT TTTATTATCA 
    CATTGGGTGC ATGGCCAGCA CTGTATCTGG TACGGCGAAT GTGGGAACTC CCCAAACGTC
    CCAGAAAAGA AGTTGAACTG CAATTACACC GGTCCTGCTG TGCCTCTGCC TAATGAAGGA
    CAGGAGCTGC TGCAGGAGCT GTGTCCGAGA CTGGTCTACG CAGACAACAG AGTTTGCTGC
    GATACACAAC AGCTCAACAC ACTAAAAAGC AACATCCAGA TCCCCCTTCA GTACCTGTCC
    AGGTGTCCAG CATGTTTCTT CAACTTCATG ACCCTGTTCT GCGAGCTCAC CTGCAGCCCC
    AGACAGAGCC AGTTTATCAG CGTAAAAGAC TTCCTTAAAG AGAAAAACCA GACCAGTGTG
    GGGAATGTGA CCTATTATAT CACCCAGACC TTTGCCGATG CCATGTTTAA CGCCTGCAGA
    GATGTCCAGG CTCCATCCAG CAACATCAAA GCTCTCGGTC TGTTGTGCGG CAGAGACGCC
    AGTGTGTGTA CTCCTCAAAT TTGGATCCAG TACATGTTTA GTATCAGCAA CGGACAGGTT
    CCCTTTGGCA TCGAACCGAT TTTCACAGAT GTCCCTGTGC AGGGCATGAC CCCAATGAAT
    AACAGGACGT TTAACTGCTC TCAGTCTCTG GATGACGGCT CGGAACCCTG CTCGTGTCAG
    GACTGCAGTG AAGTGTGCGG GCCCACGCCT GTGCCCCCTC CCATCCCTCC TCCCTGGATT
    ATCCTCGGCC TGGATGCCAT GAGCTTCATC ATGTGGTGCT CTTACATCGC TTTCCTCCTC
    ATCTTCTTCG GAGTGGTTCT GGGAGCTTGG TGTTACAGAA GGAGCGTGGT CACATCTGAA
    TATGGCCCAA TTCTTGACAG CAATCAGCCC CACTCCTTAA ACTCTGATGG CACAGATTTA
    ATAGATGAGG CGTCGTGCTG TGAGACTGTG GGCGAGCGTT TCGAAAACTC TCTGCGTCTG
    GTGTTCAGCC GCTGGGGCTC TCTGTGCGTC CGGCAGCCGC TCACCATCAT CTTGAGCAGT
    CTGGTGCTGA TCTGCATCTG CTCAGCCGGA CTCTCCTACA TGCGCATCAC CACTAACCCT
    GTGGAGCTCT GGTCTGCTCC CAGCAGCAGA GCACGGCAGG AGAAAAACTA CTTCGACCAG
    CACTTCGGGC CCTTCTTCCG CACCGAGCAG CTCATCATCA CCACTCCATG GACCGAGGAG
    GGAGGTTTCT CCACCATCAC AGGAGACATC ATTCCCTTCT CCCCCATTCT GAACCTATCA
    CTGCTGCATC AGGTGCTGGA TCTGCAGTTG GAAATCGAGA ATCTGATCGC TGAATATAAA
    GGAGAGAATG TGACTCTGAA AGACATCTGT GTTTCACCTT TGTCTCCTTA CAATGACAAC
    TGCACCATCC TGAGTGTGTT AAACTACTTC CAGAACAGCC ATGAAGTCCT GGATCATGAG
    TTTCAAGACG AATTCTTCCT CTACAATGAC TACCACACTC ATCTGCTCTA CTGTGCCAGT
    TCCCCGACCT CTCTGGATGA CACGAGCCGT TTGCATGACC CCTGCATGGG CACGTTCGGA
    GGTCCAGTTT TCCCCTGGCT TGTTCTTGGA GGATATGAGG ACTCCGCCTA CAACAATGCC
    ACCGCTCTGG TGATCACTTT TCCTGTCACC AACTACCTGA ATGACACTGA AAAACTAGGC
    AAAGCTTTGG CATGGGAGAA AGAGTTTATT AGGTTTATGA AGAATTACGA GAACCCGAAC
    CTCACGGTGT CCTTCAGCTC CGAGCGCAGT ATCGAGGACG AGATTGACCG CGAGAGCAAC
    AGTGATGTTT CCACCATCGT GATCAGCTAC ATCATCATGT TTGTCTACAT CTCAGTGGCT
    TTGGGCCGCA TCAACAGCTG CAGGACACTG CTGGTGGACT CTAAGATCTC TCTGGGCATC
    GCTGGGATCC TGATCGTGTT GAGTTCAGTG GCGTGTTCGC TGGGGATCTT CAGCTACATT
    GGGATCCCGC TCACGCTTAT CGTCATTGAG GTCATTCCCT TCCTCGTACT GGCCGTAGGA
    GTGGACAACA TCTTTATCAT TGTGCAGACC TATCAGAGAG ACGAGCGAAT GCCAGAGGAG
    GAGCTTCATC AGCAGATTGG CCGAATCCTC GGAGATGTGG CTCCCAGCAT GTTCCTCTCT
    TCCTTCTCTG AGACCGTGGC CTTCTTTTTA GGTGCTCTGT CCACCATGCC TGCGGTGAGG
    ACGTTCTCTC TGTTCGCTGG TCTGGCCATC TTTATCGACT TCCTCCTGCA GATCAGCTGC
    TTTGTGTCTT TATTGGGCCT CGACATCAAG AGACAAGAGG CCAATCGGAT GGATATATTG
    TGCTGTGTGA AGTTGTCTGA TGGACAAGAA GAAAAATCTG AAGGTTGGCT CTTCCGCTTC
    TTCAAGAAAA TATACGCTCC TTTCATCCTG AAGGACTGGG TTCGCCCTCT GGTGGTTGCT
    GTGTTTGTGG GAATGCTGTC CTTCAGCATT GCCGTTGTTA ATAAAGTGGA GATTGGACTG
    GAGCAGACGC TATCCATGCC TGATGACTCA TATGTGCTGA ATTACTTTGG GAATCTGAGC
    AAGTATCTAC ACACCGGGCC GCCGGTTTAC TTCGTGGTTG AAGATGGCCA TGATTATAAG
    ACGTTTGAGG GCCAGAACGC AGTGTGTGGT GGAGTCGGCT GCAACAACGA CTCTCTGGTC
    CAGCAGATTT ACACCGCTTC CCTCATGAGC AACTACACAA GGATCAGTAA TGTACCCTCG
    TCCTGGCTGG ATGACTACTT TGACTGGGTG AAGCCGCAGT CGACCTGCTG CAGATATTAC
    AACAGCACAG GAGCTTTCTG TAACGCTTCA GTGGTGGACA AGAGCTGCGT TCACTGCAGA
    CCAATGACCT CTAGTGGGAA ACAGAGACCG AACGGCACTG AGTTCATGCA CTTCCTCCCC
    ATGTTTCTCT CTGACAACCC CAATATCAAA TGTGGAAAAG GTGGTCATGC AGCGTACGGC
    ACTGCCGTGG ATCTCAAAGA CAACAACACA GATGTGGGCG CCACATACTT CATGAGTTAC
    CACACCATCC TGAAAAACTC CTCCGACTTT ATTAATGCCA TGAAGATGGC CAGAGAGCTT
    ACAGACAACA TCACACAGAC CCTCAGCACG CATGACAAGA GCTACAAAGT CTTCCCTTAC
    AGTGTGTTTT ACGTCTTCTA CGAGCAGTAT CTGACCATCG TCGATGACAC CGCGCTGAAC
    CTCGGCGTGT CTCTGTCCGC CATCTTCATT GTGACGGCAG TTCTGCTGGG CTTTGAGCTC
    TGGTCGGCGG TGCTCGTCTG CTTCACCATC GCTATGATCC TCATCAACAT GTTCGGGGTC
    ATGTGGCTCT GGAGCATCAG CCTCAACGCT GTCTCATTGG TCAACTTGGT CATGAGTTGT
    GGGATATCAG TGGAGTTCTG CAGTCATATA GTGAGGGCTT TCTCCATCAG CACCAGGAGC
    TCCCGGGTGG AGCGGGCAGA GGAGGCGTTG GCACATATGG GCAGCTCCGT CTTCAGTGGA
    ATCACCCTCA CCAAGTTTGG CGGGATCCTG ATTCTCGCTC TGTCCAAATC GCAGATCTTC
    CAGATTTTCT ACTTCCGCAT GTATCTGGCA ATCGTGCTTC TGGGAGCCGC TCACGGCCTC
    ATCTTCCTGC CTGTCTTACT CAGTTATGCA GGTAAATCAC TATCTCCGAG AACATTCATG
    ATTTGTCTTG AAGCAGTAGT TCATTTAAAA CATTTAAAGA CAAGGCATCA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005163715.1
    CDS88..3921
    Translation

    Target ORF information:

    RefSeq Version XM_005163658.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Niemann-Pick disease, type C1 (npc1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005163658.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCTTCTGC TGGGGAGAAA CCACATTTTC CGTCTTTTAT TGGCCACAGT TTTATTATCA 
    CATTGGGTGC ATGGCCAGCA CTGTATCTGG TACGGCGAAT GTGGGAACTC CCCAAACGTC
    CCAGAAAAGA AGTTGAACTG CAATTACACC GGTCCTGCTG TGCCTCTGCC TAATGAAGGA
    CAGGAGCTGC TGCAGGAGCT GTGTCCGAGA CTGGTCTACG CAGACAACAG AGTTTGCTGC
    GATACACAAC AGCTCAACAC ACTAAAAAGC AACATCCAGA TCCCCCTTCA GTACCTGTCC
    AGGTGTCCAG CATGTTTCTT CAACTTCATG ACCCTGTTCT GCGAGCTCAC CTGCAGCCCC
    AGACAGAGCC AGTTTATCAG CGTAAAAGAC TTCCTTAAAG AGAAAAACCA GACCAGTGTG
    GGGAATGTGA CCTATTATAT CACCCAGACC TTTGCCGATG CCATGTTTAA CGCCTGCAGA
    GATGTCCAGG CTCCATCCAG CAACATCAAA GCTCTCGGTC TGTTGTGCGG CAGAGACGCC
    AGTGTGTGTA CTCCTCAAAT TTGGATCCAG TACATGTTTA GTATCAGCAA CGGACAGGTT
    CCCTTTGGCA TCGAACCGAT TTTCACAGAT GTCCCTGTGC AGGGCATGAC CCCAATGAAT
    AACAGGACGT TTAACTGCTC TCAGTCTCTG GATGACGGCT CGGAACCCTG CTCGTGTCAG
    GACTGCAGTG AAGTGTGCGG GCCCACGCCT GTGCCCCCTC CCATCCCTCC TCCCTGGATT
    ATCCTCGGCC TGGATGCCAT GAGCTTCATC ATGTGGTGCT CTTACATCGC TTTCCTCCTC
    ATCTTCTTCG GAGTGGTTCT GGGAGCTTGG TGTTACAGAA GGAGCGTGGT CACATCTGAA
    TATGGCCCAA TTCTTGACAG CAATCAGCCC CACTCCTTAA ACTCTGATGG CACAGATTTA
    ATAGATGAGG CGTCGTGCTG TGAGACTGTG GGCGAGCGTT TCGAAAACTC TCTGCGTCTG
    GTGTTCAGCC GCTGGGGCTC TCTGTGCGTC CGGCAGCCGC TCACCATCAT CTTGAGCAGT
    CTGGTGCTGA TCTGCATCTG CTCAGCCGGA CTCTCCTACA TGCGCATCAC CACTAACCCT
    GTGGAGCTCT GGTCTGCTCC CAGCAGCAGA GCACGGCAGG AGAAAAACTA CTTCGACCAG
    CACTTCGGGC CCTTCTTCCG CACCGAGCAG CTCATCATCA CCACTCCATG GACCGAGGAG
    GGAGGTTTCT CCACCATCAC AGGAGACATC ATTCCCTTCT CCCCCATTCT GAACCTATCA
    CTGCTGCATC AGGTGCTGGA TCTGCAGTTG GAAATCGAGA ATCTGATCGC TGAATATAAA
    GGAGAGAATG TGACTCTGAA AGACATCTGT GTTTCACCTT TGTCTCCTTA CAATGACAAC
    TGCACCATCC TGAGTGTGTT AAACTACTTC CAGAACAGCC ATGAAGTCCT GGATCATGAG
    TTTCAAGACG AATTCTTCCT CTACAATGAC TACCACACTC ATCTGCTCTA CTGTGCCAGT
    TCCCCGACCT CTCTGGATGA CACGAGCCGT TTGCATGACC CCTGCATGGG CACGTTCGGA
    GGTCCAGTTT TCCCCTGGCT TGTTCTTGGA GGATATGAGG ACTCCGCCTA CAACAATGCC
    ACCGCTCTGG TGATCACTTT TCCTGTCACC AACTACCTGA ATGACACTGA AAAACTAGGC
    AAAGCTTTGG CATGGGAGAA AGAGTTTATT AGGTTTATGA AGAATTACGA GAACCCGAAC
    CTCACGGTGT CCTTCAGCTC CGAGCGCAGT ATCGAGGACG AGATTGACCG CGAGAGCAAC
    AGTGATGTTT CCACCATCGT GATCAGCTAC ATCATCATGT TTGTCTACAT CTCAGTGGCT
    TTGGGCCGCA TCAACAGCTG CAGGACACTG CTGGTGGACT CTAAGATCTC TCTGGGCATC
    GCTGGGATCC TGATCGTGTT GAGTTCAGTG GCGTGTTCGC TGGGGATCTT CAGCTACATT
    GGGATCCCGC TCACGCTTAT CGTCATTGAG GTCATTCCCT TCCTCGTACT GGCCGTAGGA
    GTGGACAACA TCTTTATCAT TGTGCAGACC TATCAGAGAG ACGAGCGAAT GCCAGAGGAG
    GAGCTTCATC AGCAGATTGG CCGAATCCTC GGAGATGTGG CTCCCAGCAT GTTCCTCTCT
    TCCTTCTCTG AGACCGTGGC CTTCTTTTTA GGTGCTCTGT CCACCATGCC TGCGGTGAGG
    ACGTTCTCTC TGTTCGCTGG TCTGGCCATC TTTATCGACT TCCTCCTGCA GATCAGCTGC
    TTTGTGTCTT TATTGGGCCT CGACATCAAG AGACAAGAGG CCAATCGGAT GGATATATTG
    TGCTGTGTGA AGTTGTCTGA TGGACAAGAA GAAAAATCTG AAGGTTGGCT CTTCCGCTTC
    TTCAAGAAAA TATACGCTCC TTTCATCCTG AAGGACTGGG TTCGCCCTCT GGTGGTTGCT
    GTGTTTGTGG GAATGCTGTC CTTCAGCATT GCCGTTGTTA ATAAAGTGGA GATTGGACTG
    GAGCAGACGC TATCCATGCC TGATGACTCA TATGTGCTGA ATTACTTTGG GAATCTGAGC
    AAGTATCTAC ACACCGGGCC GCCGGTTTAC TTCGTGGTTG AAGATGGCCA TGATTATAAG
    ACGTTTGAGG GCCAGAACGC AGTGTGTGGT GGAGTCGGCT GCAACAACGA CTCTCTGGTC
    CAGCAGATTT ACACCGCTTC CCTCATGAGC AACTACACAA GGATCAGTAA TGTACCCTCG
    TCCTGGCTGG ATGACTACTT TGACTGGGTG AAGCCGCAGT CGACCTGCTG CAGATATTAC
    AACAGCACAG GAGCTTTCTG TAACGCTTCA GTGGTGGACA AGAGCTGCGT TCACTGCAGA
    CCAATGACCT CTAGTGGGAA ACAGAGACCG AACGGCACTG AGTTCATGCA CTTCCTCCCC
    ATGTTTCTCT CTGACAACCC CAATATCAAA TGTGGAAAAG GTGGTCATGC AGCGTACGGC
    ACTGCCGTGG ATCTCAAAGA CAACAACACA GATGTGGGCG CCACATACTT CATGAGTTAC
    CACACCATCC TGAAAAACTC CTCCGACTTT ATTAATGCCA TGAAGATGGC CAGAGAGCTT
    ACAGACAACA TCACACAGAC CCTCAGCACG CATGACAAGA GCTACAAAGT CTTCCCTTAC
    AGTGTGTTTT ACGTCTTCTA CGAGCAGTAT CTGACCATCG TCGATGACAC CGCGCTGAAC
    CTCGGCGTGT CTCTGTCCGC CATCTTCATT GTGACGGCAG TTCTGCTGGG CTTTGAGCTC
    TGGTCGGCGG TGCTCGTCTG CTTCACCATC GCTATGATCC TCATCAACAT GTTCGGGGTC
    ATGTGGCTCT GGAGCATCAG CCTCAACGCT GTCTCATTGG TCAACTTGGT CATGAGTTGT
    GGGATATCAG TGGAGTTCTG CAGTCATATA GTGAGGGCTT TCTCCATCAG CACCAGGAGC
    TCCCGGGTGG AGCGGGCAGA GGAGGCGTTG GCACATATGG GCAGCTCCGT CTTCAGTGGA
    ATCACCCTCA CCAAGTTTGG CGGGATCCTG ATTCTCGCTC TGTCCAAATC GCAGATCTTC
    CAGATTTTCT ACTTCCGCAT GTATCTGGCA ATCGTGCTTC TGGGAGCCGC TCACGGCCTC
    ATCTTCCTGC CTGTCTTACT CAGTTATGCA GGTAAATCAC TATCTCCGAG AACATTCATG
    ATTTGTCTTG AAGCAGTAGT TCATTTAAAA CATTTAAAGA CAAGGCATCA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa06626
    Clone ID Related Accession (Same CDS sequence) XM_005163659.4 , XM_005163659.3
    Accession Version XM_005163659.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3831bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product Niemann-Pick C1 protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005163659.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCTTCTGC TGGGGAGAAA CCACATTTTC CGTCTTTTAT TGGCCACAGT TTTATTATCA 
    CATTGGGTGC ATGGCCAGCA CTGTATCTGG TACGGCGAAT GTGGGAACTC CCCAAACGTC
    CCAGAAAAGA AGTTGAACTG CAATTACACC GGTCCTGCTG TGCCTCTGCC TAATGAAGGA
    CAGGAGCTGC TGCAGGAGCT GTGTCCGAGA CTGGTCTACG CAGACAACAG AGTTTGCTGC
    GATACACAAC AGCTCAACAC ACTAAAAAGC AACATCCAGA TCCCCCTTCA GTACCTGTCC
    AGGTGTCCAG CATGTTTCTT CAACTTCATG ACCCTGTTCT GCGAGCTCAC CTGCAGCCCC
    AGACAGAGCC AGTTTATCAG CGTAAAAGAC TTCCTTAAAG AGAAAAACCA GACCAGTGTG
    GGGAATGTGA CCTATTATAT CACCCAGACC TTTGCCGATG CCATGTTTAA CGCCTGCAGA
    GATGTCCAGG CTCCATCCAG CAACATCAAA GCTCTCGGTC TGTTGTGCGG CAGAGACGCC
    AGTGTGTGTA CTCCTCAAAT TTGGATCCAG TACATGTTTA GTATCAGCAA CGGACAGGTT
    CCCTTTGGCA TCGAACCGAT TTTCACAGAT GTCCCTGTGC AGGGCATGAC CCCAATGAAT
    AACAGGACGT TTAACTGCTC TCAGTCTCTG GATGACGGCT CGGAACCCTG CTCGTGTCAG
    GACTGCAGTG AAGTGTGCGG GCCCACGCCT GTGCCCCCTC CCATCCCTCC TCCCTGGATT
    ATCCTCGGCC TGGATGCCAT GAGCTTCATC ATGTGGTGCT CTTACATCGC TTTCCTCCTC
    ATCTTCTTCG GAGTGGTTCT GGGAGCTTGG TGTTACAGAA GGAGCGTGGT CACATCTGAA
    TATGGCCCAA TTCTTGACAG CAATCAGCCC CACTCCTTAA ACTCTGATGG CACAGATTTA
    ATAGATGAGG CGTCGTGCTG TGAGACTGTG GGCGAGCGTT TCGAAAACTC TCTGCGTCTG
    GTGTTCAGCC GCTGGGGCTC TCTGTGCGTC CGGCAGCCGC TCACCATCAT CTTGAGCAGT
    CTGGTGCTGA TCTGCATCTG CTCAGCCGGA CTCTCCTACA TGCGCATCAC CACTAACCCT
    GTGGAGCTCT GGTCTGCTCC CAGCAGCAGA GCACGGCAGG AGAAAAACTA CTTCGACCAG
    CACTTCGGGC CCTTCTTCCG CACCGAGCAG CTCATCATCA CCACTCCATG GACCGAGGAG
    GGAGGTTTCT CCACCATCAC AGGAGACATC ATTCCCTTCT CCCCCATTCT GAACCTATCA
    CTGCTGCATC AGGTGCTGGA TCTGCAGTTG GAAATCGAGA ATCTGATCGC TGAATATAAA
    GGAGAGAATG TGACTCTGAA AGACATCTGT GTTTCACCTT TGTCTCCTTA CAATGACAAC
    TGCACCATCC TGAGTGTGTT AAACTACTTC CAGAACAGCC ATGAAGTCCT GGATCATGAG
    TTTCAAGACG AATTCTTCCT CTACAATGAC TACCACACTC ATCTGCTCTA CTGTGCCAGT
    TCCCCGACCT CTCTGGATGA CACGAGCCGT TTGCATGACC CCTGCATGGG CACGTTCGGA
    GGTCCAGTTT TCCCCTGGCT TGTTCTTGGA GGATATGAGG ACTCCGCCTA CAACAATGCC
    ACCGCTCTGG TGATCACTTT TCCTGTCACC AACTACCTGA ATGACACTGA AAAACTAGGC
    AAAGCTTTGG CATGGGAGAA AGAGTTTATT AGGTTTATGA AGAATTACGA GAACCCGAAC
    CTCACGGTGT CCTTCAGCTC CGAGCGCAGT ATCGAGGACG AGATTGACCG CGAGAGCAAC
    AGTGATGTTT CCACCATCGT GATCAGCTAC ATCATCATGT TTGTCTACAT CTCAGTGGCT
    TTGGGCCGCA TCAACAGCTG CAGGACACTG CTGGTGGACT CTAAGATCTC TCTGGGCATC
    GCTGGGATCC TGATCGTGTT GAGTTCAGTG GCGTGTTCGC TGGGGATCTT CAGCTACATT
    GGGATCCCGC TCACGCTTAT CGTCATTGAG GTCATTCCCT TCCTCGTACT GGCCGTAGGA
    GTGGACAACA TCTTTATCAT TGTGCAGACC TATCAGAGAG ACGAGCGAAT GCCAGAGGAG
    GAGCTTCATC AGCAGATTGG CCGAATCCTC GGAGATGTGG CTCCCAGCAT GTTCCTCTCT
    TCCTTCTCTG AGACCGTGGC CTTCTTTTTA GGTGCTCTGT CCACCATGCC TGCGGTGAGG
    ACGTTCTCTC TGTTCGCTGG TCTGGCCATC TTTATCGACT TCCTCCTGCA GATCAGCTGC
    TTTGTGTCTT TATTGGGCCT CGACATCAAG AGACAAGAGG CCAATCGGAT GGATATATTG
    TGCTGTGTGA AGTTGTCTGA TGGACAAGAA GAAAAATCTG AAGGTTGGCT CTTCCGCTTC
    TTCAAGAAAA TATACGCTCC TTTCATCCTG AAGGACTGGG TTCGCCCTCT GGTGGTTGCT
    GTGTTTGTGG GAATGCTGTC CTTCAGCATT GCCGTTGTTA ATAAAGTGGA GATTGGACTG
    GAGCAGACGC TATCCATGCC TGATGACTCA TATGTGCTGA ATTACTTTGG GAATCTGAGC
    AAGTATCTAC ACACCGGGCC GCCGGTTTAC TTCGTGGTTG AAGATGGCCA TGATTATAAG
    ACGTTTGAGG GCCAGAACGC AGTGTGTGGT GGAGTCGGCT GCAACAACGA CTCTCTGGTC
    CAGCAGATTT ACACCGCTTC CCTCATGAGC AACTACACAA GGATCAGTAA TGTACCCTCG
    TCCTGGCTGG ATGACTACTT TGACTGGGTG AAGCCGCAGT CGACCTGCTG CAGATATTAC
    AACAGCACAG GAGCTTTCTG TAACGCTTCA GTGGTGGACA AGAGCTGCGT TCACTGCAGA
    CCAATGACCT CTAGTGGGAA ACAGAGACCG AACGGCACTG AGTTCATGCA CTTCCTCCCC
    ATGTTTCTCT CTGACAACCC CAATATCAAA TGTGGAAAAG GTGGTCATGC AGCGTACGGC
    ACTGCCGTGG ATCTCAAAGA CAACAACACA GATGTGGGCG CCACATACTT CATGAGTTAC
    CACACCATCC TGAAAAACTC CTCCGACTTT ATTAATGCCA TGAAGATGGC CAGAGAGCTT
    ACAGACAACA TCACACAGAC CCTCAGCACG CATGACAAGA GCTACAAAGT CTTCCCTTAC
    AGTGTGTTTT ACGTCTTCTA CGAGCAGTAT CTGACCATCG TCGATGACAC CGCGCTGAAC
    CTCGGCGTGT CTCTGTCCGC CATCTTCATT GTGACGGCAG TTCTGCTGGG CTTTGAGCTC
    TGGTCGGCGG TGCTCGTCTG CTTCACCATC GCTATGATCC TCATCAACAT GTTCGGGGTC
    ATGTGGCTCT GGAGCATCAG CCTCAACGCT GTCTCATTGG TCAACTTGGT CATGAGTTGT
    GGGATATCAG TGGAGTTCTG CAGTCATATA GTGAGGGCTT TCTCCATCAG CACCAGGAGC
    TCCCGGGTGG AGCGGGCAGA GGAGGCGTTG GCACATATGG GCAGCTCCGT CTTCAGTGGA
    ATCACCCTCA CCAAGTTTGG CGGGATCCTG ATTCTCGCTC TGTCCAAATC GCAGATCTTC
    CAGATTTTCT ACTTCCGCAT GTATCTGGCA ATCGTGCTTC TGGGAGCCGC TCACGGCCTC
    ATCTTCCTGC CTGTCTTACT CAGTTATGCA GGCCCGTCTG TAAATAAAGC AAAGGTTCTA
    GCTGCGCACA ACAGGTTTGT GGGCACAGAG AGAGAGCGGC TCATCTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005163716.1
    CDS88..3918
    Translation

    Target ORF information:

    RefSeq Version XM_005163659.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Niemann-Pick disease, type C1 (npc1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005163659.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCTTCTGC TGGGGAGAAA CCACATTTTC CGTCTTTTAT TGGCCACAGT TTTATTATCA 
    CATTGGGTGC ATGGCCAGCA CTGTATCTGG TACGGCGAAT GTGGGAACTC CCCAAACGTC
    CCAGAAAAGA AGTTGAACTG CAATTACACC GGTCCTGCTG TGCCTCTGCC TAATGAAGGA
    CAGGAGCTGC TGCAGGAGCT GTGTCCGAGA CTGGTCTACG CAGACAACAG AGTTTGCTGC
    GATACACAAC AGCTCAACAC ACTAAAAAGC AACATCCAGA TCCCCCTTCA GTACCTGTCC
    AGGTGTCCAG CATGTTTCTT CAACTTCATG ACCCTGTTCT GCGAGCTCAC CTGCAGCCCC
    AGACAGAGCC AGTTTATCAG CGTAAAAGAC TTCCTTAAAG AGAAAAACCA GACCAGTGTG
    GGGAATGTGA CCTATTATAT CACCCAGACC TTTGCCGATG CCATGTTTAA CGCCTGCAGA
    GATGTCCAGG CTCCATCCAG CAACATCAAA GCTCTCGGTC TGTTGTGCGG CAGAGACGCC
    AGTGTGTGTA CTCCTCAAAT TTGGATCCAG TACATGTTTA GTATCAGCAA CGGACAGGTT
    CCCTTTGGCA TCGAACCGAT TTTCACAGAT GTCCCTGTGC AGGGCATGAC CCCAATGAAT
    AACAGGACGT TTAACTGCTC TCAGTCTCTG GATGACGGCT CGGAACCCTG CTCGTGTCAG
    GACTGCAGTG AAGTGTGCGG GCCCACGCCT GTGCCCCCTC CCATCCCTCC TCCCTGGATT
    ATCCTCGGCC TGGATGCCAT GAGCTTCATC ATGTGGTGCT CTTACATCGC TTTCCTCCTC
    ATCTTCTTCG GAGTGGTTCT GGGAGCTTGG TGTTACAGAA GGAGCGTGGT CACATCTGAA
    TATGGCCCAA TTCTTGACAG CAATCAGCCC CACTCCTTAA ACTCTGATGG CACAGATTTA
    ATAGATGAGG CGTCGTGCTG TGAGACTGTG GGCGAGCGTT TCGAAAACTC TCTGCGTCTG
    GTGTTCAGCC GCTGGGGCTC TCTGTGCGTC CGGCAGCCGC TCACCATCAT CTTGAGCAGT
    CTGGTGCTGA TCTGCATCTG CTCAGCCGGA CTCTCCTACA TGCGCATCAC CACTAACCCT
    GTGGAGCTCT GGTCTGCTCC CAGCAGCAGA GCACGGCAGG AGAAAAACTA CTTCGACCAG
    CACTTCGGGC CCTTCTTCCG CACCGAGCAG CTCATCATCA CCACTCCATG GACCGAGGAG
    GGAGGTTTCT CCACCATCAC AGGAGACATC ATTCCCTTCT CCCCCATTCT GAACCTATCA
    CTGCTGCATC AGGTGCTGGA TCTGCAGTTG GAAATCGAGA ATCTGATCGC TGAATATAAA
    GGAGAGAATG TGACTCTGAA AGACATCTGT GTTTCACCTT TGTCTCCTTA CAATGACAAC
    TGCACCATCC TGAGTGTGTT AAACTACTTC CAGAACAGCC ATGAAGTCCT GGATCATGAG
    TTTCAAGACG AATTCTTCCT CTACAATGAC TACCACACTC ATCTGCTCTA CTGTGCCAGT
    TCCCCGACCT CTCTGGATGA CACGAGCCGT TTGCATGACC CCTGCATGGG CACGTTCGGA
    GGTCCAGTTT TCCCCTGGCT TGTTCTTGGA GGATATGAGG ACTCCGCCTA CAACAATGCC
    ACCGCTCTGG TGATCACTTT TCCTGTCACC AACTACCTGA ATGACACTGA AAAACTAGGC
    AAAGCTTTGG CATGGGAGAA AGAGTTTATT AGGTTTATGA AGAATTACGA GAACCCGAAC
    CTCACGGTGT CCTTCAGCTC CGAGCGCAGT ATCGAGGACG AGATTGACCG CGAGAGCAAC
    AGTGATGTTT CCACCATCGT GATCAGCTAC ATCATCATGT TTGTCTACAT CTCAGTGGCT
    TTGGGCCGCA TCAACAGCTG CAGGACACTG CTGGTGGACT CTAAGATCTC TCTGGGCATC
    GCTGGGATCC TGATCGTGTT GAGTTCAGTG GCGTGTTCGC TGGGGATCTT CAGCTACATT
    GGGATCCCGC TCACGCTTAT CGTCATTGAG GTCATTCCCT TCCTCGTACT GGCCGTAGGA
    GTGGACAACA TCTTTATCAT TGTGCAGACC TATCAGAGAG ACGAGCGAAT GCCAGAGGAG
    GAGCTTCATC AGCAGATTGG CCGAATCCTC GGAGATGTGG CTCCCAGCAT GTTCCTCTCT
    TCCTTCTCTG AGACCGTGGC CTTCTTTTTA GGTGCTCTGT CCACCATGCC TGCGGTGAGG
    ACGTTCTCTC TGTTCGCTGG TCTGGCCATC TTTATCGACT TCCTCCTGCA GATCAGCTGC
    TTTGTGTCTT TATTGGGCCT CGACATCAAG AGACAAGAGG CCAATCGGAT GGATATATTG
    TGCTGTGTGA AGTTGTCTGA TGGACAAGAA GAAAAATCTG AAGGTTGGCT CTTCCGCTTC
    TTCAAGAAAA TATACGCTCC TTTCATCCTG AAGGACTGGG TTCGCCCTCT GGTGGTTGCT
    GTGTTTGTGG GAATGCTGTC CTTCAGCATT GCCGTTGTTA ATAAAGTGGA GATTGGACTG
    GAGCAGACGC TATCCATGCC TGATGACTCA TATGTGCTGA ATTACTTTGG GAATCTGAGC
    AAGTATCTAC ACACCGGGCC GCCGGTTTAC TTCGTGGTTG AAGATGGCCA TGATTATAAG
    ACGTTTGAGG GCCAGAACGC AGTGTGTGGT GGAGTCGGCT GCAACAACGA CTCTCTGGTC
    CAGCAGATTT ACACCGCTTC CCTCATGAGC AACTACACAA GGATCAGTAA TGTACCCTCG
    TCCTGGCTGG ATGACTACTT TGACTGGGTG AAGCCGCAGT CGACCTGCTG CAGATATTAC
    AACAGCACAG GAGCTTTCTG TAACGCTTCA GTGGTGGACA AGAGCTGCGT TCACTGCAGA
    CCAATGACCT CTAGTGGGAA ACAGAGACCG AACGGCACTG AGTTCATGCA CTTCCTCCCC
    ATGTTTCTCT CTGACAACCC CAATATCAAA TGTGGAAAAG GTGGTCATGC AGCGTACGGC
    ACTGCCGTGG ATCTCAAAGA CAACAACACA GATGTGGGCG CCACATACTT CATGAGTTAC
    CACACCATCC TGAAAAACTC CTCCGACTTT ATTAATGCCA TGAAGATGGC CAGAGAGCTT
    ACAGACAACA TCACACAGAC CCTCAGCACG CATGACAAGA GCTACAAAGT CTTCCCTTAC
    AGTGTGTTTT ACGTCTTCTA CGAGCAGTAT CTGACCATCG TCGATGACAC CGCGCTGAAC
    CTCGGCGTGT CTCTGTCCGC CATCTTCATT GTGACGGCAG TTCTGCTGGG CTTTGAGCTC
    TGGTCGGCGG TGCTCGTCTG CTTCACCATC GCTATGATCC TCATCAACAT GTTCGGGGTC
    ATGTGGCTCT GGAGCATCAG CCTCAACGCT GTCTCATTGG TCAACTTGGT CATGAGTTGT
    GGGATATCAG TGGAGTTCTG CAGTCATATA GTGAGGGCTT TCTCCATCAG CACCAGGAGC
    TCCCGGGTGG AGCGGGCAGA GGAGGCGTTG GCACATATGG GCAGCTCCGT CTTCAGTGGA
    ATCACCCTCA CCAAGTTTGG CGGGATCCTG ATTCTCGCTC TGTCCAAATC GCAGATCTTC
    CAGATTTTCT ACTTCCGCAT GTATCTGGCA ATCGTGCTTC TGGGAGCCGC TCACGGCCTC
    ATCTTCCTGC CTGTCTTACT CAGTTATGCA GGCCCGTCTG TAAATAAAGC AAAGGTTCTA
    GCTGCGCACA ACAGGTTTGT GGGCACAGAG AGAGAGCGGC TCATCTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa06626
    Clone ID Related Accession (Same CDS sequence) XM_005163659.4 , XM_005163659.3
    Accession Version XM_005163659.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3831bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product Niemann-Pick C1 protein isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005163659.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCTTCTGC TGGGGAGAAA CCACATTTTC CGTCTTTTAT TGGCCACAGT TTTATTATCA 
    CATTGGGTGC ATGGCCAGCA CTGTATCTGG TACGGCGAAT GTGGGAACTC CCCAAACGTC
    CCAGAAAAGA AGTTGAACTG CAATTACACC GGTCCTGCTG TGCCTCTGCC TAATGAAGGA
    CAGGAGCTGC TGCAGGAGCT GTGTCCGAGA CTGGTCTACG CAGACAACAG AGTTTGCTGC
    GATACACAAC AGCTCAACAC ACTAAAAAGC AACATCCAGA TCCCCCTTCA GTACCTGTCC
    AGGTGTCCAG CATGTTTCTT CAACTTCATG ACCCTGTTCT GCGAGCTCAC CTGCAGCCCC
    AGACAGAGCC AGTTTATCAG CGTAAAAGAC TTCCTTAAAG AGAAAAACCA GACCAGTGTG
    GGGAATGTGA CCTATTATAT CACCCAGACC TTTGCCGATG CCATGTTTAA CGCCTGCAGA
    GATGTCCAGG CTCCATCCAG CAACATCAAA GCTCTCGGTC TGTTGTGCGG CAGAGACGCC
    AGTGTGTGTA CTCCTCAAAT TTGGATCCAG TACATGTTTA GTATCAGCAA CGGACAGGTT
    CCCTTTGGCA TCGAACCGAT TTTCACAGAT GTCCCTGTGC AGGGCATGAC CCCAATGAAT
    AACAGGACGT TTAACTGCTC TCAGTCTCTG GATGACGGCT CGGAACCCTG CTCGTGTCAG
    GACTGCAGTG AAGTGTGCGG GCCCACGCCT GTGCCCCCTC CCATCCCTCC TCCCTGGATT
    ATCCTCGGCC TGGATGCCAT GAGCTTCATC ATGTGGTGCT CTTACATCGC TTTCCTCCTC
    ATCTTCTTCG GAGTGGTTCT GGGAGCTTGG TGTTACAGAA GGAGCGTGGT CACATCTGAA
    TATGGCCCAA TTCTTGACAG CAATCAGCCC CACTCCTTAA ACTCTGATGG CACAGATTTA
    ATAGATGAGG CGTCGTGCTG TGAGACTGTG GGCGAGCGTT TCGAAAACTC TCTGCGTCTG
    GTGTTCAGCC GCTGGGGCTC TCTGTGCGTC CGGCAGCCGC TCACCATCAT CTTGAGCAGT
    CTGGTGCTGA TCTGCATCTG CTCAGCCGGA CTCTCCTACA TGCGCATCAC CACTAACCCT
    GTGGAGCTCT GGTCTGCTCC CAGCAGCAGA GCACGGCAGG AGAAAAACTA CTTCGACCAG
    CACTTCGGGC CCTTCTTCCG CACCGAGCAG CTCATCATCA CCACTCCATG GACCGAGGAG
    GGAGGTTTCT CCACCATCAC AGGAGACATC ATTCCCTTCT CCCCCATTCT GAACCTATCA
    CTGCTGCATC AGGTGCTGGA TCTGCAGTTG GAAATCGAGA ATCTGATCGC TGAATATAAA
    GGAGAGAATG TGACTCTGAA AGACATCTGT GTTTCACCTT TGTCTCCTTA CAATGACAAC
    TGCACCATCC TGAGTGTGTT AAACTACTTC CAGAACAGCC ATGAAGTCCT GGATCATGAG
    TTTCAAGACG AATTCTTCCT CTACAATGAC TACCACACTC ATCTGCTCTA CTGTGCCAGT
    TCCCCGACCT CTCTGGATGA CACGAGCCGT TTGCATGACC CCTGCATGGG CACGTTCGGA
    GGTCCAGTTT TCCCCTGGCT TGTTCTTGGA GGATATGAGG ACTCCGCCTA CAACAATGCC
    ACCGCTCTGG TGATCACTTT TCCTGTCACC AACTACCTGA ATGACACTGA AAAACTAGGC
    AAAGCTTTGG CATGGGAGAA AGAGTTTATT AGGTTTATGA AGAATTACGA GAACCCGAAC
    CTCACGGTGT CCTTCAGCTC CGAGCGCAGT ATCGAGGACG AGATTGACCG CGAGAGCAAC
    AGTGATGTTT CCACCATCGT GATCAGCTAC ATCATCATGT TTGTCTACAT CTCAGTGGCT
    TTGGGCCGCA TCAACAGCTG CAGGACACTG CTGGTGGACT CTAAGATCTC TCTGGGCATC
    GCTGGGATCC TGATCGTGTT GAGTTCAGTG GCGTGTTCGC TGGGGATCTT CAGCTACATT
    GGGATCCCGC TCACGCTTAT CGTCATTGAG GTCATTCCCT TCCTCGTACT GGCCGTAGGA
    GTGGACAACA TCTTTATCAT TGTGCAGACC TATCAGAGAG ACGAGCGAAT GCCAGAGGAG
    GAGCTTCATC AGCAGATTGG CCGAATCCTC GGAGATGTGG CTCCCAGCAT GTTCCTCTCT
    TCCTTCTCTG AGACCGTGGC CTTCTTTTTA GGTGCTCTGT CCACCATGCC TGCGGTGAGG
    ACGTTCTCTC TGTTCGCTGG TCTGGCCATC TTTATCGACT TCCTCCTGCA GATCAGCTGC
    TTTGTGTCTT TATTGGGCCT CGACATCAAG AGACAAGAGG CCAATCGGAT GGATATATTG
    TGCTGTGTGA AGTTGTCTGA TGGACAAGAA GAAAAATCTG AAGGTTGGCT CTTCCGCTTC
    TTCAAGAAAA TATACGCTCC TTTCATCCTG AAGGACTGGG TTCGCCCTCT GGTGGTTGCT
    GTGTTTGTGG GAATGCTGTC CTTCAGCATT GCCGTTGTTA ATAAAGTGGA GATTGGACTG
    GAGCAGACGC TATCCATGCC TGATGACTCA TATGTGCTGA ATTACTTTGG GAATCTGAGC
    AAGTATCTAC ACACCGGGCC GCCGGTTTAC TTCGTGGTTG AAGATGGCCA TGATTATAAG
    ACGTTTGAGG GCCAGAACGC AGTGTGTGGT GGAGTCGGCT GCAACAACGA CTCTCTGGTC
    CAGCAGATTT ACACCGCTTC CCTCATGAGC AACTACACAA GGATCAGTAA TGTACCCTCG
    TCCTGGCTGG ATGACTACTT TGACTGGGTG AAGCCGCAGT CGACCTGCTG CAGATATTAC
    AACAGCACAG GAGCTTTCTG TAACGCTTCA GTGGTGGACA AGAGCTGCGT TCACTGCAGA
    CCAATGACCT CTAGTGGGAA ACAGAGACCG AACGGCACTG AGTTCATGCA CTTCCTCCCC
    ATGTTTCTCT CTGACAACCC CAATATCAAA TGTGGAAAAG GTGGTCATGC AGCGTACGGC
    ACTGCCGTGG ATCTCAAAGA CAACAACACA GATGTGGGCG CCACATACTT CATGAGTTAC
    CACACCATCC TGAAAAACTC CTCCGACTTT ATTAATGCCA TGAAGATGGC CAGAGAGCTT
    ACAGACAACA TCACACAGAC CCTCAGCACG CATGACAAGA GCTACAAAGT CTTCCCTTAC
    AGTGTGTTTT ACGTCTTCTA CGAGCAGTAT CTGACCATCG TCGATGACAC CGCGCTGAAC
    CTCGGCGTGT CTCTGTCCGC CATCTTCATT GTGACGGCAG TTCTGCTGGG CTTTGAGCTC
    TGGTCGGCGG TGCTCGTCTG CTTCACCATC GCTATGATCC TCATCAACAT GTTCGGGGTC
    ATGTGGCTCT GGAGCATCAG CCTCAACGCT GTCTCATTGG TCAACTTGGT CATGAGTTGT
    GGGATATCAG TGGAGTTCTG CAGTCATATA GTGAGGGCTT TCTCCATCAG CACCAGGAGC
    TCCCGGGTGG AGCGGGCAGA GGAGGCGTTG GCACATATGG GCAGCTCCGT CTTCAGTGGA
    ATCACCCTCA CCAAGTTTGG CGGGATCCTG ATTCTCGCTC TGTCCAAATC GCAGATCTTC
    CAGATTTTCT ACTTCCGCAT GTATCTGGCA ATCGTGCTTC TGGGAGCCGC TCACGGCCTC
    ATCTTCCTGC CTGTCTTACT CAGTTATGCA GGCCCGTCTG TAAATAAAGC AAAGGTTCTA
    GCTGCGCACA ACAGGTTTGT GGGCACAGAG AGAGAGCGGC TCATCTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005163716.1
    CDS91..3921
    Translation

    Target ORF information:

    RefSeq Version XM_005163659.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Niemann-Pick disease, type C1 (npc1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005163659.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCTTCTGC TGGGGAGAAA CCACATTTTC CGTCTTTTAT TGGCCACAGT TTTATTATCA 
    CATTGGGTGC ATGGCCAGCA CTGTATCTGG TACGGCGAAT GTGGGAACTC CCCAAACGTC
    CCAGAAAAGA AGTTGAACTG CAATTACACC GGTCCTGCTG TGCCTCTGCC TAATGAAGGA
    CAGGAGCTGC TGCAGGAGCT GTGTCCGAGA CTGGTCTACG CAGACAACAG AGTTTGCTGC
    GATACACAAC AGCTCAACAC ACTAAAAAGC AACATCCAGA TCCCCCTTCA GTACCTGTCC
    AGGTGTCCAG CATGTTTCTT CAACTTCATG ACCCTGTTCT GCGAGCTCAC CTGCAGCCCC
    AGACAGAGCC AGTTTATCAG CGTAAAAGAC TTCCTTAAAG AGAAAAACCA GACCAGTGTG
    GGGAATGTGA CCTATTATAT CACCCAGACC TTTGCCGATG CCATGTTTAA CGCCTGCAGA
    GATGTCCAGG CTCCATCCAG CAACATCAAA GCTCTCGGTC TGTTGTGCGG CAGAGACGCC
    AGTGTGTGTA CTCCTCAAAT TTGGATCCAG TACATGTTTA GTATCAGCAA CGGACAGGTT
    CCCTTTGGCA TCGAACCGAT TTTCACAGAT GTCCCTGTGC AGGGCATGAC CCCAATGAAT
    AACAGGACGT TTAACTGCTC TCAGTCTCTG GATGACGGCT CGGAACCCTG CTCGTGTCAG
    GACTGCAGTG AAGTGTGCGG GCCCACGCCT GTGCCCCCTC CCATCCCTCC TCCCTGGATT
    ATCCTCGGCC TGGATGCCAT GAGCTTCATC ATGTGGTGCT CTTACATCGC TTTCCTCCTC
    ATCTTCTTCG GAGTGGTTCT GGGAGCTTGG TGTTACAGAA GGAGCGTGGT CACATCTGAA
    TATGGCCCAA TTCTTGACAG CAATCAGCCC CACTCCTTAA ACTCTGATGG CACAGATTTA
    ATAGATGAGG CGTCGTGCTG TGAGACTGTG GGCGAGCGTT TCGAAAACTC TCTGCGTCTG
    GTGTTCAGCC GCTGGGGCTC TCTGTGCGTC CGGCAGCCGC TCACCATCAT CTTGAGCAGT
    CTGGTGCTGA TCTGCATCTG CTCAGCCGGA CTCTCCTACA TGCGCATCAC CACTAACCCT
    GTGGAGCTCT GGTCTGCTCC CAGCAGCAGA GCACGGCAGG AGAAAAACTA CTTCGACCAG
    CACTTCGGGC CCTTCTTCCG CACCGAGCAG CTCATCATCA CCACTCCATG GACCGAGGAG
    GGAGGTTTCT CCACCATCAC AGGAGACATC ATTCCCTTCT CCCCCATTCT GAACCTATCA
    CTGCTGCATC AGGTGCTGGA TCTGCAGTTG GAAATCGAGA ATCTGATCGC TGAATATAAA
    GGAGAGAATG TGACTCTGAA AGACATCTGT GTTTCACCTT TGTCTCCTTA CAATGACAAC
    TGCACCATCC TGAGTGTGTT AAACTACTTC CAGAACAGCC ATGAAGTCCT GGATCATGAG
    TTTCAAGACG AATTCTTCCT CTACAATGAC TACCACACTC ATCTGCTCTA CTGTGCCAGT
    TCCCCGACCT CTCTGGATGA CACGAGCCGT TTGCATGACC CCTGCATGGG CACGTTCGGA
    GGTCCAGTTT TCCCCTGGCT TGTTCTTGGA GGATATGAGG ACTCCGCCTA CAACAATGCC
    ACCGCTCTGG TGATCACTTT TCCTGTCACC AACTACCTGA ATGACACTGA AAAACTAGGC
    AAAGCTTTGG CATGGGAGAA AGAGTTTATT AGGTTTATGA AGAATTACGA GAACCCGAAC
    CTCACGGTGT CCTTCAGCTC CGAGCGCAGT ATCGAGGACG AGATTGACCG CGAGAGCAAC
    AGTGATGTTT CCACCATCGT GATCAGCTAC ATCATCATGT TTGTCTACAT CTCAGTGGCT
    TTGGGCCGCA TCAACAGCTG CAGGACACTG CTGGTGGACT CTAAGATCTC TCTGGGCATC
    GCTGGGATCC TGATCGTGTT GAGTTCAGTG GCGTGTTCGC TGGGGATCTT CAGCTACATT
    GGGATCCCGC TCACGCTTAT CGTCATTGAG GTCATTCCCT TCCTCGTACT GGCCGTAGGA
    GTGGACAACA TCTTTATCAT TGTGCAGACC TATCAGAGAG ACGAGCGAAT GCCAGAGGAG
    GAGCTTCATC AGCAGATTGG CCGAATCCTC GGAGATGTGG CTCCCAGCAT GTTCCTCTCT
    TCCTTCTCTG AGACCGTGGC CTTCTTTTTA GGTGCTCTGT CCACCATGCC TGCGGTGAGG
    ACGTTCTCTC TGTTCGCTGG TCTGGCCATC TTTATCGACT TCCTCCTGCA GATCAGCTGC
    TTTGTGTCTT TATTGGGCCT CGACATCAAG AGACAAGAGG CCAATCGGAT GGATATATTG
    TGCTGTGTGA AGTTGTCTGA TGGACAAGAA GAAAAATCTG AAGGTTGGCT CTTCCGCTTC
    TTCAAGAAAA TATACGCTCC TTTCATCCTG AAGGACTGGG TTCGCCCTCT GGTGGTTGCT
    GTGTTTGTGG GAATGCTGTC CTTCAGCATT GCCGTTGTTA ATAAAGTGGA GATTGGACTG
    GAGCAGACGC TATCCATGCC TGATGACTCA TATGTGCTGA ATTACTTTGG GAATCTGAGC
    AAGTATCTAC ACACCGGGCC GCCGGTTTAC TTCGTGGTTG AAGATGGCCA TGATTATAAG
    ACGTTTGAGG GCCAGAACGC AGTGTGTGGT GGAGTCGGCT GCAACAACGA CTCTCTGGTC
    CAGCAGATTT ACACCGCTTC CCTCATGAGC AACTACACAA GGATCAGTAA TGTACCCTCG
    TCCTGGCTGG ATGACTACTT TGACTGGGTG AAGCCGCAGT CGACCTGCTG CAGATATTAC
    AACAGCACAG GAGCTTTCTG TAACGCTTCA GTGGTGGACA AGAGCTGCGT TCACTGCAGA
    CCAATGACCT CTAGTGGGAA ACAGAGACCG AACGGCACTG AGTTCATGCA CTTCCTCCCC
    ATGTTTCTCT CTGACAACCC CAATATCAAA TGTGGAAAAG GTGGTCATGC AGCGTACGGC
    ACTGCCGTGG ATCTCAAAGA CAACAACACA GATGTGGGCG CCACATACTT CATGAGTTAC
    CACACCATCC TGAAAAACTC CTCCGACTTT ATTAATGCCA TGAAGATGGC CAGAGAGCTT
    ACAGACAACA TCACACAGAC CCTCAGCACG CATGACAAGA GCTACAAAGT CTTCCCTTAC
    AGTGTGTTTT ACGTCTTCTA CGAGCAGTAT CTGACCATCG TCGATGACAC CGCGCTGAAC
    CTCGGCGTGT CTCTGTCCGC CATCTTCATT GTGACGGCAG TTCTGCTGGG CTTTGAGCTC
    TGGTCGGCGG TGCTCGTCTG CTTCACCATC GCTATGATCC TCATCAACAT GTTCGGGGTC
    ATGTGGCTCT GGAGCATCAG CCTCAACGCT GTCTCATTGG TCAACTTGGT CATGAGTTGT
    GGGATATCAG TGGAGTTCTG CAGTCATATA GTGAGGGCTT TCTCCATCAG CACCAGGAGC
    TCCCGGGTGG AGCGGGCAGA GGAGGCGTTG GCACATATGG GCAGCTCCGT CTTCAGTGGA
    ATCACCCTCA CCAAGTTTGG CGGGATCCTG ATTCTCGCTC TGTCCAAATC GCAGATCTTC
    CAGATTTTCT ACTTCCGCAT GTATCTGGCA ATCGTGCTTC TGGGAGCCGC TCACGGCCTC
    ATCTTCCTGC CTGTCTTACT CAGTTATGCA GGCCCGTCTG TAAATAAAGC AAAGGTTCTA
    GCTGCGCACA ACAGGTTTGT GGGCACAGAG AGAGAGCGGC TCATCTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.