npc1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	npc1
Entrez Gene ID	553330
Full Name	Niemann-Pick disease, type C1
Synonyms	im:7149020,wu:fb53a12,wu:fc29a12
General protein information	Preferred Names Niemann-Pick disease, type C1 Names Niemann-Pick C1 protein
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005163658.2	XP_005163715.1	Niemann-Pick C1 protein isoform X1
XM_005163658.3	XP_005163715.1	Niemann-Pick C1 protein isoform X1
XM_005163659.4	XP_005163716.1	Niemann-Pick C1 protein isoform X2
XM_005163659.3	XP_005163716.1	Niemann-Pick C1 protein isoform X2
XM_005163658.4	XP_005163715.1	Niemann-Pick C1 protein isoform X1
NM_001243875.1	NP_001230804.1	Niemann-Pick C1 protein precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NPC1	NP_001003107.1
Bos taurus (cattle)	NPC1	NP_777183.1
Kluyveromyces lactis	KLLA0F27137g	XP_456287.1
Magnaporthe oryzae (rice blast fungus)	MGG_00819	XP_003718164.1
Mus musculus (house mouse)	Npc1	NP_032746.2
Drosophila melanogaster (fruit fly)	Npc1a	NP_001188769.1
Homo sapiens (human)	NPC1	NP_000262.2
Pan troglodytes (chimpanzee)	NPC1	XP_001155285.1
	NPC1	XP_002800934.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008137	XP_317322.4
Rattus norvegicus (Norway rat)	Npc1	NP_705888.2
Danio rerio (zebrafish)	npc1	NP_001230804.1
Saccharomyces cerevisiae (baker's yeast)	NCR1	NP_015319.1
Eremothecium gossypii	AGOS_AGL008W	NP_986658.1
Neurospora crassa	NCU04090	XP_956086.1
Arabidopsis thaliana (thale cress)	AT1G42470	NP_174975.2
Gallus gallus (chicken)	NPC1	XP_419162.3
Arabidopsis thaliana (thale cress)	AT4G38350	NP_001190953.1
Xenopus tropicalis (tropical clawed frog)	npc1	XP_004915269.1

Gene Ontology
Bibliography

Related articles in PubMed

Pla2g6 Deficiency in Zebrafish Leads to Dopaminergic Cell Death, Axonal Degeneration, Increased ?-Synuclein Expression, and Defects in Brain Functions and Pathways.
S?nchez E, Azcona LJ, Pais?n-Ruiz C
Molecular neurobiology(2018 Jan)

Dietary lipid content reorganizes gut microbiota and probiotic L. rhamnosus attenuates obesity and enhances catabolic hormonal milieu in zebrafish.
Falcinelli S, Rodiles A, Hatef A, Picchietti S, Cossignani L, Merrifield DL, Unniappan S, Carnevali O
Scientific reports7(1)5512(2017 Jul)

Altered Adipogenesis in Zebrafish Larvae Following High Fat Diet and Chemical Exposure Is Visualised by Stimulated Raman Scattering Microscopy.
den Broeder MJ, Moester MJB, Kamstra JH, Cenijn PH, Davidoiu V, Kamminga LM, Ariese F, de Boer JF, Legler J
International journal of molecular sciences18(4)(2017 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

this is the first report, showing a role of NPC1 in platelet function and formation but further studies are needed to define how cholesterol storage interferes with these processes
Title: NPC1 defect results in abnormal platelet formation and function: studies in Niemann-Pick disease type C1 patients and zebrafish.

npc1 is required early for proper cell movement and cholesterol localization and later for cell survival
Title: Requirement of Npc1 and availability of cholesterol for early embryonic cell movements in zebrafish.

The following npc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the npc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa31915	NM_001243875.1 Latest version!	Danio rerio Niemann-Pick disease, type C1 (npc1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa06625	XM_005163658.4 Latest version!	Danio rerio Niemann-Pick disease, type C1 (npc1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa06626	XM_005163659.4 Latest version!	Danio rerio Niemann-Pick disease, type C1 (npc1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
ODa06625	XM_005163658.2	Danio rerio Niemann-Pick disease, type C1 (npc1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa06626	XM_005163659.3	Danio rerio Niemann-Pick disease, type C1 (npc1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa06625	XM_005163658.3	Danio rerio Niemann-Pick disease, type C1 (npc1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

npc1 ( NM_001243875.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001230804.1 Danio rerio Niemann-Pick disease, type C1 (npc1), mRNA.

Price & Availability↑

CloneID	ODa31915
Clone ID Related Accession (Same CDS sequence)	NM_001243875.1
Accession Version	NM_001243875.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-09
Organism	Danio rerio(zebrafish)
Product	Niemann-Pick C1 protein precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FP245481.6 and FP236166.8. On Sep 2, 2011 this sequence version replaced XM_001919923.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCTTCTGC TGGGGAGAAA CCACATTTTC CGTCTTTTAT TGGCCACAGT TTTATTATCA 
CATTGGGTGC ATGGCCAGCA CTGTATCTGG TACGGCGAAT GTGGGAACTC CCCAAACGTC 
CCAGAAAAGA AGTTGAACTG CAATTACACC GGTCCTGCTG TGCCTCTGCC TAATGAAGGA 
CAGGAGCTGC TGCAGGAGCT GTGTCCGAGA CTGGTCTACG CAGACAACAG AGTTTGCTGC 
GATACACAAC AGCTCAACAC ACTAAAAAGC AACATCCAGA TCCCCCTTCA GTACCTGTCC 
AGGTGTCCAG CATGTTTCTT CAACTTCATG ACCCTGTTCT GCGAGCTCAC CTGCAGCCCC 
AGACAGAGCC AGTTTATCAG CGTAAAAGAC TTCCTTAAAG AGAAAAACCA GACCAGTGTG 
GGGAATGTGA CCTATTATAT CACCCAGACC TTTGCCGATG CCATGTTTAA CGCCTGCAGA 
GATGTCCAGG CTCCATCCAG CAACATCAAA GCTCTCGGTC TGTTGTGCGG CAGAGACGCC 
AGTGTGTGTA CTCCTCAAAT TTGGATCCAG TACATGTTTA GTATCAGCAA CGGACAGGTT 
CCCTTTGGCA TCGAACCGAT TTTCACAGAT GTCCCTGTGC AGGGCATGAC CCCAATGAAT 
AACAGGACGT TTAACTGCTC TCAGTCTCTG GATGACGGCT CGGAACCCTG CTCGTGTCAG 
GACTGCAGTG AAGTGTGCGG GCCCACGCCT GTGCCCCCTC CCATCCCTCC TCCCTGGATT 
ATCCTCGGCC TGGATGCCAT GAGCTTCATC ATGTGGTGCT CTTACATCGC TTTCCTCCTC 
ATCTTCTTCG GAGTGGTTCT GGGAGCTTGG TGTTACAGAA GGAGCGTGGT CACATCTGAA 
TATGGCCCAA TTCTTGACAG CAATCAGCCC CACTCCTTAA ACTCTGATGA TGAGGCGTCG 
TGCTGTGAGA CTGTGGGCGA GCGTTTCGAA AACTCTCTGC GTCTGGTGTT CAGCCGCTGG 
GGCTCTCTGT GCGTCCGGCA GCCGCTCACC ATCATCTTGA GCAGTCTGGT GCTGATCTGC 
ATCTGCTCAG CCGGACTCTC CTACATGCGC ATCACCACTA ACCCTGTGGA GCTCTGGTCT 
GCTCCCAGCA GCAGAGCACG GCAGGAGAAA AACTACTTCG ACCAGCACTT CGGGCCCTTC 
TTCCGCACCG AGCAGCTCAT CATCACCACT CCATGGACCG AGGAGGGAGG TTTCTCCACC 
ATCACAGGAG ACATCATTCC CTTCTCCCCC ATTCTGAACC TATCACTGCT GCATCAGGTG 
CTGGATCTGC AGTTGGAAAT CGAGAATCTG ATCGCTGAAT ATAAAGGAGA GAATGTGACT 
CTGAAAGACA TCTGTGTTTC ACCTTTGTCT CCTTACAATG ACAACTGCAC CATCCTGAGT 
GTGTTAAACT ACTTCCAGAA CAGCCATGAA GTCCTGGATC ATGAGTTTCA AGACGAATTC 
TTCCTCTACA ATGACTACCA CACTCATCTG CTCTACTGTG CCAGTTCCCC GACCTCTCTG 
GATGACACGA GCCGTTTGCA TGACCCCTGC ATGGGCACGT TCGGAGGTCC AGTTTTCCCC 
TGGCTTGTTC TTGGAGGATA TGAGGACTCC GCCTACAACA ATGCCACCGC TCTGGTGATC 
ACTTTTCCTG TCACCAACTA CCTGAATGAC ACTGAAAAAC TAGGCAAAGC TTTGGCATGG 
GAGAAAGAGT TTATTAGGTT TATGAAGAAT TACGAGAACC CGAACCTCAC GGTGTCCTTC 
AGCTCCGAGC GCAGTATCGA GGACGAGATT GACCGCGAGA GCAACAGTGA TGTTTCCACC 
ATCGTGATCA GCTACATCAT CATGTTTGTC TACATCTCAG TGGCTTTGGG CCGCATCAAC 
AGCTGCAGGA CACTGCTGGT GGACTCTAAG ATCTCTCTGG GCATCGCTGG GATCCTGATC 
GTGTTGAGTT CAGTGGCGTG TTCGCTGGGG ATCTTCAGCT ACATTGGGAT CCCGCTCACG 
CTTATCGTCA TTGAGGTCAT TCCCTTCCTC GTACTGGCCG TAGGAGTGGA CAACATCTTT 
ATCATTGTGC AGACCTATCA GAGAGACGAG CGAATGCCAG AGGAGGAGCT TCATCAGCAG 
ATTGGCCGAA TCCTCGGAGA TGTGGCTCCC AGCATGTTCC TCTCTTCCTT CTCTGAGACC 
GTGGCCTTCT TTTTAGGTGC TCTGTCCACC ATGCCTGCGG TGAGGACGTT CTCTCTGTTC 
GCTGGTCTGG CCATCTTTAT CGACTTCCTC CTGCAGATCA GCTGCTTTGT GTCTTTATTG 
GGCCTCGACA TCAAGAGACA AGAGGCCAAT CGGATGGATA TATTGTGCTG TGTGAAGTTG 
TCTGATGGAC AAGAAGAAAA ATCTGAAGGT TGGCTCTTCC GCTTCTTCAA GAAAATATAC 
GCTCCTTTCA TCCTGAAGGA CTGGGTTCGC CCTCTGGTGG TTGCTGTGTT TGTGGGAATG 
CTGTCCTTCA GCATTGCCGT TGTTAATAAA GTGGAGATTG GACTGGAGCA GACGCTATCC 
ATGCCTGATG ACTCATATGT GCTGAATTAC TTTGGGAATC TGAGCAAGTA TCTACACACC 
GGGCCGCCGG TTTACTTCGT GGTTGAAGAT GGCCATGATT ATAAGACGTT TGAGGGCCAG 
AACGCAGTGT GTGGTGGAGT CGGCTGCAAC AACGACTCTC TGGTCCAGCA GATTTACACC 
GCTTCCCTCA TGAGCAACTA CACAAGGATC AGTAATGTAC CCTCGTCCTG GCTGGATGAC 
TACTTTGACT GGGTGAAGCC GCAGTCGACC TGCTGCAGAT ATTACAACAG CACAGGAGCT 
TTCTGTAACG CTTCAGTGGT GGACAAGAGC TGCGTTCACT GCAGACCAAT GACCTCTAGT 
GGGAAACAGA GACCGAACGG CACTGAGTTC ATGCACTTCC TCCCCATGTT TCTCTCTGAC 
AACCCCAATA TCAAATGTGG AAAAGGTGGT CATGCAGCGT ACGGCACTGC CGTGGATCTC 
AAAGACAACA ACACAGATGT GGGCGCCACA TACTTCATGA GTTACCACAC CATCCTGAAA 
AACTCCTCCG ACTTTATTAA TGCCATGAAG ATGGCCAGAG AGCTTACAGA CAACATCACA 
CAGACCCTCA GCACGCATGA CAAGAGCTAC AAAGTCTTCC CTTACAGTGT GTTTTACGTC 
TTCTACGAGC AGTATCTGAC CATCGTCGAT GACACCGCGC TGAACCTCGG CGTGTCTCTG 
TCCGCCATCT TCATTGTGAC GGCAGTTCTG CTGGGCTTTG AGCTCTGGTC GGCGGTGCTC 
GTCTGCTTCA CCATCGCTAT GATCCTCATC AACATGTTCG GGGTCATGTG GCTCTGGAGC 
ATCAGCCTCA ACGCTGTCTC ATTGGTCAAC TTGGTCATGA GTTGTGGGAT ATCAGTGGAG 
TTCTGCAGTC ATATAGTGAG GGCTTTCTCC ATCAGCACCA GGAGCTCCCG GGTGGAGCGG 
GCAGAGGAGG CGTTGGCACA TATGGGCAGC TCCGTCTTCA GTGGAATCAC CCTCACCAAG 
TTTGGCGGGA TCCTGATTCT CGCTCTGTCC AAATCGCAGA TCTTCCAGAT TTTCTACTTC 
CGCATGTATC TGGCAATCGT GCTTCTGGGA GCCGCTCACG GCCTCATCTT CCTGCCTGTC 
TTACTCAGTT ATGCAGGCCC GTCTGTAAAT AAAGCAAAGG TTCTAGCTGC GCACAACAGG 
TTTGTGGGCA CAGAGAGAGA GCGGCTCATC TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001230804.1
CDS	42..3857
Translation	MLLLGRNHIFRLLLATVLLSHWVHGQHCIWYGECGNSPNVPEKKLNCNYTGPAVPLPNEGQELLQELCPRLVYADNRVCCDTQQLNTLKSNIQIPLQYLSRCPACFFNFMTLFCELTCSPRQSQFISVKDFLKEKNQTSVGNVTYYITQTFADAMFNACRDVQAPSSNIKALGLLCGRDASVCTPQIWIQYMFSISNGQVPFGIEPIFTDVPVQGMTPMNNRTFNCSQSLDDGSEPCSCQDCSEVCGPTPVPPPIPPPWIILGLDAMSFIMWCSYIAFLLIFFGVVLGAWCYRRSVVTSEYGPILDSNQPHSLNSDDEASCCETVGERFENSLRLVFSRWGSLCVRQPLTIILSSLVLICICSAGLSYMRITTNPVELWSAPSSRARQEKNYFDQHFGPFFRTEQLIITTPWTEEGGFSTITGDIIPFSPILNLSLLHQVLDLQLEIENLIAEYKGENVTLKDICVSPLSPYNDNCTILSVLNYFQNSHEVLDHEFQDEFFLYNDYHTHLLYCASSPTSLDDTSRLHDPCMGTFGGPVFPWLVLGGYEDSAYNNATALVITFPVTNYLNDTEKLGKALAWEKEFIRFMKNYENPNLTVSFSSERSIEDEIDRESNSDVSTIVISYIIMFVYISVALGRINSCRTLLVDSKISLGIAGILIVLSSVACSLGIFSYIGIPLTLIVIEVIPFLVLAVGVDNIFIIVQTYQRDERMPEEELHQQIGRILGDVAPSMFLSSFSETVAFFLGALSTMPAVRTFSLFAGLAIFIDFLLQISCFVSLLGLDIKRQEANRMDILCCVKLSDGQEEKSEGWLFRFFKKIYAPFILKDWVRPLVVAVFVGMLSFSIAVVNKVEIGLEQTLSMPDDSYVLNYFGNLSKYLHTGPPVYFVVEDGHDYKTFEGQNAVCGGVGCNNDSLVQQIYTASLMSNYTRISNVPSSWLDDYFDWVKPQSTCCRYYNSTGAFCNASVVDKSCVHCRPMTSSGKQRPNGTEFMHFLPMFLSDNPNIKCGKGGHAAYGTAVDLKDNNTDVGATYFMSYHTILKNSSDFINAMKMARELTDNITQTLSTHDKSYKVFPYSVFYVFYEQYLTIVDDTALNLGVSLSAIFIVTAVLLGFELWSAVLVCFTIAMILINMFGVMWLWSISLNAVSLVNLVMSCGISVEFCSHIVRAFSISTRSSRVERAEEALAHMGSSVFSGITLTKFGGILILALSKSQIFQIFYFRMYLAIVLLGAAHGLIFLPVLLSYAGPSVNKAKVLAAHNRFVGTERERLIY

Target ORF information:

RefSeq Version	NM_001243875.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Niemann-Pick disease, type C1 (npc1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001243875.1

ORF Insert Sequence:

ATGCTTCTGC TGGGGAGAAA CCACATTTTC CGTCTTTTAT TGGCCACAGT TTTATTATCA 
CATTGGGTGC ATGGCCAGCA CTGTATCTGG TACGGCGAAT GTGGGAACTC CCCAAACGTC 
CCAGAAAAGA AGTTGAACTG CAATTACACC GGTCCTGCTG TGCCTCTGCC TAATGAAGGA 
CAGGAGCTGC TGCAGGAGCT GTGTCCGAGA CTGGTCTACG CAGACAACAG AGTTTGCTGC 
GATACACAAC AGCTCAACAC ACTAAAAAGC AACATCCAGA TCCCCCTTCA GTACCTGTCC 
AGGTGTCCAG CATGTTTCTT CAACTTCATG ACCCTGTTCT GCGAGCTCAC CTGCAGCCCC 
AGACAGAGCC AGTTTATCAG CGTAAAAGAC TTCCTTAAAG AGAAAAACCA GACCAGTGTG 
GGGAATGTGA CCTATTATAT CACCCAGACC TTTGCCGATG CCATGTTTAA CGCCTGCAGA 
GATGTCCAGG CTCCATCCAG CAACATCAAA GCTCTCGGTC TGTTGTGCGG CAGAGACGCC 
AGTGTGTGTA CTCCTCAAAT TTGGATCCAG TACATGTTTA GTATCAGCAA CGGACAGGTT 
CCCTTTGGCA TCGAACCGAT TTTCACAGAT GTCCCTGTGC AGGGCATGAC CCCAATGAAT 
AACAGGACGT TTAACTGCTC TCAGTCTCTG GATGACGGCT CGGAACCCTG CTCGTGTCAG 
GACTGCAGTG AAGTGTGCGG GCCCACGCCT GTGCCCCCTC CCATCCCTCC TCCCTGGATT 
ATCCTCGGCC TGGATGCCAT GAGCTTCATC ATGTGGTGCT CTTACATCGC TTTCCTCCTC 
ATCTTCTTCG GAGTGGTTCT GGGAGCTTGG TGTTACAGAA GGAGCGTGGT CACATCTGAA 
TATGGCCCAA TTCTTGACAG CAATCAGCCC CACTCCTTAA ACTCTGATGA TGAGGCGTCG 
TGCTGTGAGA CTGTGGGCGA GCGTTTCGAA AACTCTCTGC GTCTGGTGTT CAGCCGCTGG 
GGCTCTCTGT GCGTCCGGCA GCCGCTCACC ATCATCTTGA GCAGTCTGGT GCTGATCTGC 
ATCTGCTCAG CCGGACTCTC CTACATGCGC ATCACCACTA ACCCTGTGGA GCTCTGGTCT 
GCTCCCAGCA GCAGAGCACG GCAGGAGAAA AACTACTTCG ACCAGCACTT CGGGCCCTTC 
TTCCGCACCG AGCAGCTCAT CATCACCACT CCATGGACCG AGGAGGGAGG TTTCTCCACC 
ATCACAGGAG ACATCATTCC CTTCTCCCCC ATTCTGAACC TATCACTGCT GCATCAGGTG 
CTGGATCTGC AGTTGGAAAT CGAGAATCTG ATCGCTGAAT ATAAAGGAGA GAATGTGACT 
CTGAAAGACA TCTGTGTTTC ACCTTTGTCT CCTTACAATG ACAACTGCAC CATCCTGAGT 
GTGTTAAACT ACTTCCAGAA CAGCCATGAA GTCCTGGATC ATGAGTTTCA AGACGAATTC 
TTCCTCTACA ATGACTACCA CACTCATCTG CTCTACTGTG CCAGTTCCCC GACCTCTCTG 
GATGACACGA GCCGTTTGCA TGACCCCTGC ATGGGCACGT TCGGAGGTCC AGTTTTCCCC 
TGGCTTGTTC TTGGAGGATA TGAGGACTCC GCCTACAACA ATGCCACCGC TCTGGTGATC 
ACTTTTCCTG TCACCAACTA CCTGAATGAC ACTGAAAAAC TAGGCAAAGC TTTGGCATGG 
GAGAAAGAGT TTATTAGGTT TATGAAGAAT TACGAGAACC CGAACCTCAC GGTGTCCTTC 
AGCTCCGAGC GCAGTATCGA GGACGAGATT GACCGCGAGA GCAACAGTGA TGTTTCCACC 
ATCGTGATCA GCTACATCAT CATGTTTGTC TACATCTCAG TGGCTTTGGG CCGCATCAAC 
AGCTGCAGGA CACTGCTGGT GGACTCTAAG ATCTCTCTGG GCATCGCTGG GATCCTGATC 
GTGTTGAGTT CAGTGGCGTG TTCGCTGGGG ATCTTCAGCT ACATTGGGAT CCCGCTCACG 
CTTATCGTCA TTGAGGTCAT TCCCTTCCTC GTACTGGCCG TAGGAGTGGA CAACATCTTT 
ATCATTGTGC AGACCTATCA GAGAGACGAG CGAATGCCAG AGGAGGAGCT TCATCAGCAG 
ATTGGCCGAA TCCTCGGAGA TGTGGCTCCC AGCATGTTCC TCTCTTCCTT CTCTGAGACC 
GTGGCCTTCT TTTTAGGTGC TCTGTCCACC ATGCCTGCGG TGAGGACGTT CTCTCTGTTC 
GCTGGTCTGG CCATCTTTAT CGACTTCCTC CTGCAGATCA GCTGCTTTGT GTCTTTATTG 
GGCCTCGACA TCAAGAGACA AGAGGCCAAT CGGATGGATA TATTGTGCTG TGTGAAGTTG 
TCTGATGGAC AAGAAGAAAA ATCTGAAGGT TGGCTCTTCC GCTTCTTCAA GAAAATATAC 
GCTCCTTTCA TCCTGAAGGA CTGGGTTCGC CCTCTGGTGG TTGCTGTGTT TGTGGGAATG 
CTGTCCTTCA GCATTGCCGT TGTTAATAAA GTGGAGATTG GACTGGAGCA GACGCTATCC 
ATGCCTGATG ACTCATATGT GCTGAATTAC TTTGGGAATC TGAGCAAGTA TCTACACACC 
GGGCCGCCGG TTTACTTCGT GGTTGAAGAT GGCCATGATT ATAAGACGTT TGAGGGCCAG 
AACGCAGTGT GTGGTGGAGT CGGCTGCAAC AACGACTCTC TGGTCCAGCA GATTTACACC 
GCTTCCCTCA TGAGCAACTA CACAAGGATC AGTAATGTAC CCTCGTCCTG GCTGGATGAC 
TACTTTGACT GGGTGAAGCC GCAGTCGACC TGCTGCAGAT ATTACAACAG CACAGGAGCT 
TTCTGTAACG CTTCAGTGGT GGACAAGAGC TGCGTTCACT GCAGACCAAT GACCTCTAGT 
GGGAAACAGA GACCGAACGG CACTGAGTTC ATGCACTTCC TCCCCATGTT TCTCTCTGAC 
AACCCCAATA TCAAATGTGG AAAAGGTGGT CATGCAGCGT ACGGCACTGC CGTGGATCTC 
AAAGACAACA ACACAGATGT GGGCGCCACA TACTTCATGA GTTACCACAC CATCCTGAAA 
AACTCCTCCG ACTTTATTAA TGCCATGAAG ATGGCCAGAG AGCTTACAGA CAACATCACA 
CAGACCCTCA GCACGCATGA CAAGAGCTAC AAAGTCTTCC CTTACAGTGT GTTTTACGTC 
TTCTACGAGC AGTATCTGAC CATCGTCGAT GACACCGCGC TGAACCTCGG CGTGTCTCTG 
TCCGCCATCT TCATTGTGAC GGCAGTTCTG CTGGGCTTTG AGCTCTGGTC GGCGGTGCTC 
GTCTGCTTCA CCATCGCTAT GATCCTCATC AACATGTTCG GGGTCATGTG GCTCTGGAGC 
ATCAGCCTCA ACGCTGTCTC ATTGGTCAAC TTGGTCATGA GTTGTGGGAT ATCAGTGGAG 
TTCTGCAGTC ATATAGTGAG GGCTTTCTCC ATCAGCACCA GGAGCTCCCG GGTGGAGCGG 
GCAGAGGAGG CGTTGGCACA TATGGGCAGC TCCGTCTTCA GTGGAATCAC CCTCACCAAG 
TTTGGCGGGA TCCTGATTCT CGCTCTGTCC AAATCGCAGA TCTTCCAGAT TTTCTACTTC 
CGCATGTATC TGGCAATCGT GCTTCTGGGA GCCGCTCACG GCCTCATCTT CCTGCCTGTC 
TTACTCAGTT ATGCAGGCCC GTCTGTAAAT AAAGCAAAGG TTCTAGCTGC GCACAACAGG 
TTTGTGGGCA CAGAGAGAGA GCGGCTCATC TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

npc1 ( XM_005163658.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005163715.1 Danio rerio Niemann-Pick disease, type C1 (npc1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa06625
Clone ID Related Accession (Same CDS sequence)	XM_005163658.2 , XM_005163658.3 , XM_005163658.4
Accession Version	XM_005163658.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-09-23
Organism	Danio rerio(Zebrafish)
Product	Niemann-Pick C1 protein isoform X1
Comment

ATGCTTCTGC TGGGGAGAAA CCACATTTTC CGTCTTTTAT TGGCCACAGT TTTATTATCA 
CATTGGGTGC ATGGCCAGCA CTGTATCTGG TACGGCGAAT GTGGGAACTC CCCAAACGTC 
CCAGAAAAGA AGTTGAACTG CAATTACACC GGTCCTGCTG TGCCTCTGCC TAATGAAGGA 
CAGGAGCTGC TGCAGGAGCT GTGTCCGAGA CTGGTCTACG CAGACAACAG AGTTTGCTGC 
GATACACAAC AGCTCAACAC ACTAAAAAGC AACATCCAGA TCCCCCTTCA GTACCTGTCC 
AGGTGTCCAG CATGTTTCTT CAACTTCATG ACCCTGTTCT GCGAGCTCAC CTGCAGCCCC 
AGACAGAGCC AGTTTATCAG CGTAAAAGAC TTCCTTAAAG AGAAAAACCA GACCAGTGTG 
GGGAATGTGA CCTATTATAT CACCCAGACC TTTGCCGATG CCATGTTTAA CGCCTGCAGA 
GATGTCCAGG CTCCATCCAG CAACATCAAA GCTCTCGGTC TGTTGTGCGG CAGAGACGCC 
AGTGTGTGTA CTCCTCAAAT TTGGATCCAG TACATGTTTA GTATCAGCAA CGGACAGGTT 
CCCTTTGGCA TCGAACCGAT TTTCACAGAT GTCCCTGTGC AGGGCATGAC CCCAATGAAT 
AACAGGACGT TTAACTGCTC TCAGTCTCTG GATGACGGCT CGGAACCCTG CTCGTGTCAG 
GACTGCAGTG AAGTGTGCGG GCCCACGCCT GTGCCCCCTC CCATCCCTCC TCCCTGGATT 
ATCCTCGGCC TGGATGCCAT GAGCTTCATC ATGTGGTGCT CTTACATCGC TTTCCTCCTC 
ATCTTCTTCG GAGTGGTTCT GGGAGCTTGG TGTTACAGAA GGAGCGTGGT CACATCTGAA 
TATGGCCCAA TTCTTGACAG CAATCAGCCC CACTCCTTAA ACTCTGATGG CACAGATTTA 
ATAGATGAGG CGTCGTGCTG TGAGACTGTG GGCGAGCGTT TCGAAAACTC TCTGCGTCTG 
GTGTTCAGCC GCTGGGGCTC TCTGTGCGTC CGGCAGCCGC TCACCATCAT CTTGAGCAGT 
CTGGTGCTGA TCTGCATCTG CTCAGCCGGA CTCTCCTACA TGCGCATCAC CACTAACCCT 
GTGGAGCTCT GGTCTGCTCC CAGCAGCAGA GCACGGCAGG AGAAAAACTA CTTCGACCAG 
CACTTCGGGC CCTTCTTCCG CACCGAGCAG CTCATCATCA CCACTCCATG GACCGAGGAG 
GGAGGTTTCT CCACCATCAC AGGAGACATC ATTCCCTTCT CCCCCATTCT GAACCTATCA 
CTGCTGCATC AGGTGCTGGA TCTGCAGTTG GAAATCGAGA ATCTGATCGC TGAATATAAA 
GGAGAGAATG TGACTCTGAA AGACATCTGT GTTTCACCTT TGTCTCCTTA CAATGACAAC 
TGCACCATCC TGAGTGTGTT AAACTACTTC CAGAACAGCC ATGAAGTCCT GGATCATGAG 
TTTCAAGACG AATTCTTCCT CTACAATGAC TACCACACTC ATCTGCTCTA CTGTGCCAGT 
TCCCCGACCT CTCTGGATGA CACGAGCCGT TTGCATGACC CCTGCATGGG CACGTTCGGA 
GGTCCAGTTT TCCCCTGGCT TGTTCTTGGA GGATATGAGG ACTCCGCCTA CAACAATGCC 
ACCGCTCTGG TGATCACTTT TCCTGTCACC AACTACCTGA ATGACACTGA AAAACTAGGC 
AAAGCTTTGG CATGGGAGAA AGAGTTTATT AGGTTTATGA AGAATTACGA GAACCCGAAC 
CTCACGGTGT CCTTCAGCTC CGAGCGCAGT ATCGAGGACG AGATTGACCG CGAGAGCAAC 
AGTGATGTTT CCACCATCGT GATCAGCTAC ATCATCATGT TTGTCTACAT CTCAGTGGCT 
TTGGGCCGCA TCAACAGCTG CAGGACACTG CTGGTGGACT CTAAGATCTC TCTGGGCATC 
GCTGGGATCC TGATCGTGTT GAGTTCAGTG GCGTGTTCGC TGGGGATCTT CAGCTACATT 
GGGATCCCGC TCACGCTTAT CGTCATTGAG GTCATTCCCT TCCTCGTACT GGCCGTAGGA 
GTGGACAACA TCTTTATCAT TGTGCAGACC TATCAGAGAG ACGAGCGAAT GCCAGAGGAG 
GAGCTTCATC AGCAGATTGG CCGAATCCTC GGAGATGTGG CTCCCAGCAT GTTCCTCTCT 
TCCTTCTCTG AGACCGTGGC CTTCTTTTTA GGTGCTCTGT CCACCATGCC TGCGGTGAGG 
ACGTTCTCTC TGTTCGCTGG TCTGGCCATC TTTATCGACT TCCTCCTGCA GATCAGCTGC 
TTTGTGTCTT TATTGGGCCT CGACATCAAG AGACAAGAGG CCAATCGGAT GGATATATTG 
TGCTGTGTGA AGTTGTCTGA TGGACAAGAA GAAAAATCTG AAGGTTGGCT CTTCCGCTTC 
TTCAAGAAAA TATACGCTCC TTTCATCCTG AAGGACTGGG TTCGCCCTCT GGTGGTTGCT 
GTGTTTGTGG GAATGCTGTC CTTCAGCATT GCCGTTGTTA ATAAAGTGGA GATTGGACTG 
GAGCAGACGC TATCCATGCC TGATGACTCA TATGTGCTGA ATTACTTTGG GAATCTGAGC 
AAGTATCTAC ACACCGGGCC GCCGGTTTAC TTCGTGGTTG AAGATGGCCA TGATTATAAG 
ACGTTTGAGG GCCAGAACGC AGTGTGTGGT GGAGTCGGCT GCAACAACGA CTCTCTGGTC 
CAGCAGATTT ACACCGCTTC CCTCATGAGC AACTACACAA GGATCAGTAA TGTACCCTCG 
TCCTGGCTGG ATGACTACTT TGACTGGGTG AAGCCGCAGT CGACCTGCTG CAGATATTAC 
AACAGCACAG GAGCTTTCTG TAACGCTTCA GTGGTGGACA AGAGCTGCGT TCACTGCAGA 
CCAATGACCT CTAGTGGGAA ACAGAGACCG AACGGCACTG AGTTCATGCA CTTCCTCCCC 
ATGTTTCTCT CTGACAACCC CAATATCAAA TGTGGAAAAG GTGGTCATGC AGCGTACGGC 
ACTGCCGTGG ATCTCAAAGA CAACAACACA GATGTGGGCG CCACATACTT CATGAGTTAC 
CACACCATCC TGAAAAACTC CTCCGACTTT ATTAATGCCA TGAAGATGGC CAGAGAGCTT 
ACAGACAACA TCACACAGAC CCTCAGCACG CATGACAAGA GCTACAAAGT CTTCCCTTAC 
AGTGTGTTTT ACGTCTTCTA CGAGCAGTAT CTGACCATCG TCGATGACAC CGCGCTGAAC 
CTCGGCGTGT CTCTGTCCGC CATCTTCATT GTGACGGCAG TTCTGCTGGG CTTTGAGCTC 
TGGTCGGCGG TGCTCGTCTG CTTCACCATC GCTATGATCC TCATCAACAT GTTCGGGGTC 
ATGTGGCTCT GGAGCATCAG CCTCAACGCT GTCTCATTGG TCAACTTGGT CATGAGTTGT 
GGGATATCAG TGGAGTTCTG CAGTCATATA GTGAGGGCTT TCTCCATCAG CACCAGGAGC 
TCCCGGGTGG AGCGGGCAGA GGAGGCGTTG GCACATATGG GCAGCTCCGT CTTCAGTGGA 
ATCACCCTCA CCAAGTTTGG CGGGATCCTG ATTCTCGCTC TGTCCAAATC GCAGATCTTC 
CAGATTTTCT ACTTCCGCAT GTATCTGGCA ATCGTGCTTC TGGGAGCCGC TCACGGCCTC 
ATCTTCCTGC CTGTCTTACT CAGTTATGCA GGTAAATCAC TATCTCCGAG AACATTCATG 
ATTTGTCTTG AAGCAGTAGT TCATTTAAAA CATTTAAAGA CAAGGCATCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_005163715.1

Translation

MLLLGRNHIFRLLLATVLLSHWVHGQHCIWYGECGNSPNVPEKKLNCNYTGPAVPLPNEGQELLQELCPRLVYADNRVCCDTQQLNTLKSNIQIPLQYLSRCPACFFNFMTLFCELTCSPRQSQFISVKDFLKEKNQTSVGNVTYYITQTFADAMFNACRDVQAPSSNIKALGLLCGRDASVCTPQIWIQYMFSISNGQVPFGIEPIFTDVPVQGMTPMNNRTFNCSQSLDDGSEPCSCQDCSEVCGPTPVPPPIPPPWIILGLDAMSFIMWCSYIAFLLIFFGVVLGAWCYRRSVVTSEYGPILDSNQPHSLNSDGTDLIDEASCCETVGERFENSLRLVFSRWGSLCVRQPLTIILSSLVLICICSAGLSYMRITTNPVELWSAPSSRARQEKNYFDQHFGPFFRTEQLIITTPWTEEGGFSTITGDIIPFSPILNLSLLHQVLDLQLEIENLIAEYKGENVTLKDICVSPLSPYNDNCTILSVLNYFQNSHEVLDHEFQDEFFLYNDYHTHLLYCASSPTSLDDTSRLHDPCMGTFGGPVFPWLVLGGYEDSAYNNATALVITFPVTNYLNDTEKLGKALAWEKEFIRFMKNYENPNLTVSFSSERSIEDEIDRESNSDVSTIVISYIIMFVYISVALGRINSCRTLLVDSKISLGIAGILIVLSSVACSLGIFSYIGIPLTLIVIEVIPFLVLAVGVDNIFIIVQTYQRDERMPEEELHQQIGRILGDVAPSMFLSSFSETVAFFLGALSTMPAVRTFSLFAGLAIFIDFLLQISCFVSLLGLDIKRQEANRMDILCCVKLSDGQEEKSEGWLFRFFKKIYAPFILKDWVRPLVVAVFVGMLSFSIAVVNKVEIGLEQTLSMPDDSYVLNYFGNLSKYLHTGPPVYFVVEDGHDYKTFEGQNAVCGGVGCNNDSLVQQIYTASLMSNYTRISNVPSSWLDDYFDWVKPQSTCCRYYNSTGAFCNASVVDKSCVHCRPMTSSGKQRPNGTEFMHFLPMFLSDNPNIKCGKGGHAAYGTAVDLKDNNTDVGATYFMSYHTILKNSSDFINAMKMARELTDNITQTLSTHDKSYKVFPYSVFYVFYEQYLTIVDDTALNLGVSLSAIFIVTAVLLGFELWSAVLVCFTIAMILINMFGVMWLWSISLNAVSLVNLVMSCGISVEFCSHIVRAFSISTRSSRVERAEEALAHMGSSVFSGITLTKFGGILILALSKSQIFQIFYFRMYLAIVLLGAAHGLIFLPVLLSYAGKSLSPRTFMICLEAVVHLKHLKTRHH

Target ORF information:

RefSeq Version	XM_005163658.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Niemann-Pick disease, type C1 (npc1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163658.2

ORF Insert Sequence:

ATGCTTCTGC TGGGGAGAAA CCACATTTTC CGTCTTTTAT TGGCCACAGT TTTATTATCA 
CATTGGGTGC ATGGCCAGCA CTGTATCTGG TACGGCGAAT GTGGGAACTC CCCAAACGTC 
CCAGAAAAGA AGTTGAACTG CAATTACACC GGTCCTGCTG TGCCTCTGCC TAATGAAGGA 
CAGGAGCTGC TGCAGGAGCT GTGTCCGAGA CTGGTCTACG CAGACAACAG AGTTTGCTGC 
GATACACAAC AGCTCAACAC ACTAAAAAGC AACATCCAGA TCCCCCTTCA GTACCTGTCC 
AGGTGTCCAG CATGTTTCTT CAACTTCATG ACCCTGTTCT GCGAGCTCAC CTGCAGCCCC 
AGACAGAGCC AGTTTATCAG CGTAAAAGAC TTCCTTAAAG AGAAAAACCA GACCAGTGTG 
GGGAATGTGA CCTATTATAT CACCCAGACC TTTGCCGATG CCATGTTTAA CGCCTGCAGA 
GATGTCCAGG CTCCATCCAG CAACATCAAA GCTCTCGGTC TGTTGTGCGG CAGAGACGCC 
AGTGTGTGTA CTCCTCAAAT TTGGATCCAG TACATGTTTA GTATCAGCAA CGGACAGGTT 
CCCTTTGGCA TCGAACCGAT TTTCACAGAT GTCCCTGTGC AGGGCATGAC CCCAATGAAT 
AACAGGACGT TTAACTGCTC TCAGTCTCTG GATGACGGCT CGGAACCCTG CTCGTGTCAG 
GACTGCAGTG AAGTGTGCGG GCCCACGCCT GTGCCCCCTC CCATCCCTCC TCCCTGGATT 
ATCCTCGGCC TGGATGCCAT GAGCTTCATC ATGTGGTGCT CTTACATCGC TTTCCTCCTC 
ATCTTCTTCG GAGTGGTTCT GGGAGCTTGG TGTTACAGAA GGAGCGTGGT CACATCTGAA 
TATGGCCCAA TTCTTGACAG CAATCAGCCC CACTCCTTAA ACTCTGATGG CACAGATTTA 
ATAGATGAGG CGTCGTGCTG TGAGACTGTG GGCGAGCGTT TCGAAAACTC TCTGCGTCTG 
GTGTTCAGCC GCTGGGGCTC TCTGTGCGTC CGGCAGCCGC TCACCATCAT CTTGAGCAGT 
CTGGTGCTGA TCTGCATCTG CTCAGCCGGA CTCTCCTACA TGCGCATCAC CACTAACCCT 
GTGGAGCTCT GGTCTGCTCC CAGCAGCAGA GCACGGCAGG AGAAAAACTA CTTCGACCAG 
CACTTCGGGC CCTTCTTCCG CACCGAGCAG CTCATCATCA CCACTCCATG GACCGAGGAG 
GGAGGTTTCT CCACCATCAC AGGAGACATC ATTCCCTTCT CCCCCATTCT GAACCTATCA 
CTGCTGCATC AGGTGCTGGA TCTGCAGTTG GAAATCGAGA ATCTGATCGC TGAATATAAA 
GGAGAGAATG TGACTCTGAA AGACATCTGT GTTTCACCTT TGTCTCCTTA CAATGACAAC 
TGCACCATCC TGAGTGTGTT AAACTACTTC CAGAACAGCC ATGAAGTCCT GGATCATGAG 
TTTCAAGACG AATTCTTCCT CTACAATGAC TACCACACTC ATCTGCTCTA CTGTGCCAGT 
TCCCCGACCT CTCTGGATGA CACGAGCCGT TTGCATGACC CCTGCATGGG CACGTTCGGA 
GGTCCAGTTT TCCCCTGGCT TGTTCTTGGA GGATATGAGG ACTCCGCCTA CAACAATGCC 
ACCGCTCTGG TGATCACTTT TCCTGTCACC AACTACCTGA ATGACACTGA AAAACTAGGC 
AAAGCTTTGG CATGGGAGAA AGAGTTTATT AGGTTTATGA AGAATTACGA GAACCCGAAC 
CTCACGGTGT CCTTCAGCTC CGAGCGCAGT ATCGAGGACG AGATTGACCG CGAGAGCAAC 
AGTGATGTTT CCACCATCGT GATCAGCTAC ATCATCATGT TTGTCTACAT CTCAGTGGCT 
TTGGGCCGCA TCAACAGCTG CAGGACACTG CTGGTGGACT CTAAGATCTC TCTGGGCATC 
GCTGGGATCC TGATCGTGTT GAGTTCAGTG GCGTGTTCGC TGGGGATCTT CAGCTACATT 
GGGATCCCGC TCACGCTTAT CGTCATTGAG GTCATTCCCT TCCTCGTACT GGCCGTAGGA 
GTGGACAACA TCTTTATCAT TGTGCAGACC TATCAGAGAG ACGAGCGAAT GCCAGAGGAG 
GAGCTTCATC AGCAGATTGG CCGAATCCTC GGAGATGTGG CTCCCAGCAT GTTCCTCTCT 
TCCTTCTCTG AGACCGTGGC CTTCTTTTTA GGTGCTCTGT CCACCATGCC TGCGGTGAGG 
ACGTTCTCTC TGTTCGCTGG TCTGGCCATC TTTATCGACT TCCTCCTGCA GATCAGCTGC 
TTTGTGTCTT TATTGGGCCT CGACATCAAG AGACAAGAGG CCAATCGGAT GGATATATTG 
TGCTGTGTGA AGTTGTCTGA TGGACAAGAA GAAAAATCTG AAGGTTGGCT CTTCCGCTTC 
TTCAAGAAAA TATACGCTCC TTTCATCCTG AAGGACTGGG TTCGCCCTCT GGTGGTTGCT 
GTGTTTGTGG GAATGCTGTC CTTCAGCATT GCCGTTGTTA ATAAAGTGGA GATTGGACTG 
GAGCAGACGC TATCCATGCC TGATGACTCA TATGTGCTGA ATTACTTTGG GAATCTGAGC 
AAGTATCTAC ACACCGGGCC GCCGGTTTAC TTCGTGGTTG AAGATGGCCA TGATTATAAG 
ACGTTTGAGG GCCAGAACGC AGTGTGTGGT GGAGTCGGCT GCAACAACGA CTCTCTGGTC 
CAGCAGATTT ACACCGCTTC CCTCATGAGC AACTACACAA GGATCAGTAA TGTACCCTCG 
TCCTGGCTGG ATGACTACTT TGACTGGGTG AAGCCGCAGT CGACCTGCTG CAGATATTAC 
AACAGCACAG GAGCTTTCTG TAACGCTTCA GTGGTGGACA AGAGCTGCGT TCACTGCAGA 
CCAATGACCT CTAGTGGGAA ACAGAGACCG AACGGCACTG AGTTCATGCA CTTCCTCCCC 
ATGTTTCTCT CTGACAACCC CAATATCAAA TGTGGAAAAG GTGGTCATGC AGCGTACGGC 
ACTGCCGTGG ATCTCAAAGA CAACAACACA GATGTGGGCG CCACATACTT CATGAGTTAC 
CACACCATCC TGAAAAACTC CTCCGACTTT ATTAATGCCA TGAAGATGGC CAGAGAGCTT 
ACAGACAACA TCACACAGAC CCTCAGCACG CATGACAAGA GCTACAAAGT CTTCCCTTAC 
AGTGTGTTTT ACGTCTTCTA CGAGCAGTAT CTGACCATCG TCGATGACAC CGCGCTGAAC 
CTCGGCGTGT CTCTGTCCGC CATCTTCATT GTGACGGCAG TTCTGCTGGG CTTTGAGCTC 
TGGTCGGCGG TGCTCGTCTG CTTCACCATC GCTATGATCC TCATCAACAT GTTCGGGGTC 
ATGTGGCTCT GGAGCATCAG CCTCAACGCT GTCTCATTGG TCAACTTGGT CATGAGTTGT 
GGGATATCAG TGGAGTTCTG CAGTCATATA GTGAGGGCTT TCTCCATCAG CACCAGGAGC 
TCCCGGGTGG AGCGGGCAGA GGAGGCGTTG GCACATATGG GCAGCTCCGT CTTCAGTGGA 
ATCACCCTCA CCAAGTTTGG CGGGATCCTG ATTCTCGCTC TGTCCAAATC GCAGATCTTC 
CAGATTTTCT ACTTCCGCAT GTATCTGGCA ATCGTGCTTC TGGGAGCCGC TCACGGCCTC 
ATCTTCCTGC CTGTCTTACT CAGTTATGCA GGTAAATCAC TATCTCCGAG AACATTCATG 
ATTTGTCTTG AAGCAGTAGT TCATTTAAAA CATTTAAAGA CAAGGCATCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

npc1 ( XM_005163658.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005163715.1 Danio rerio Niemann-Pick disease, type C1 (npc1), transcript variant X1, mRNA.

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CloneID	ODa06625
Clone ID Related Accession (Same CDS sequence)	XM_005163658.2 , XM_005163658.3 , XM_005163658.4
Accession Version	XM_005163658.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	Niemann-Pick C1 protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005163658.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCTGC TGGGGAGAAA CCACATTTTC CGTCTTTTAT TGGCCACAGT TTTATTATCA 
CATTGGGTGC ATGGCCAGCA CTGTATCTGG TACGGCGAAT GTGGGAACTC CCCAAACGTC 
CCAGAAAAGA AGTTGAACTG CAATTACACC GGTCCTGCTG TGCCTCTGCC TAATGAAGGA 
CAGGAGCTGC TGCAGGAGCT GTGTCCGAGA CTGGTCTACG CAGACAACAG AGTTTGCTGC 
GATACACAAC AGCTCAACAC ACTAAAAAGC AACATCCAGA TCCCCCTTCA GTACCTGTCC 
AGGTGTCCAG CATGTTTCTT CAACTTCATG ACCCTGTTCT GCGAGCTCAC CTGCAGCCCC 
AGACAGAGCC AGTTTATCAG CGTAAAAGAC TTCCTTAAAG AGAAAAACCA GACCAGTGTG 
GGGAATGTGA CCTATTATAT CACCCAGACC TTTGCCGATG CCATGTTTAA CGCCTGCAGA 
GATGTCCAGG CTCCATCCAG CAACATCAAA GCTCTCGGTC TGTTGTGCGG CAGAGACGCC 
AGTGTGTGTA CTCCTCAAAT TTGGATCCAG TACATGTTTA GTATCAGCAA CGGACAGGTT 
CCCTTTGGCA TCGAACCGAT TTTCACAGAT GTCCCTGTGC AGGGCATGAC CCCAATGAAT 
AACAGGACGT TTAACTGCTC TCAGTCTCTG GATGACGGCT CGGAACCCTG CTCGTGTCAG 
GACTGCAGTG AAGTGTGCGG GCCCACGCCT GTGCCCCCTC CCATCCCTCC TCCCTGGATT 
ATCCTCGGCC TGGATGCCAT GAGCTTCATC ATGTGGTGCT CTTACATCGC TTTCCTCCTC 
ATCTTCTTCG GAGTGGTTCT GGGAGCTTGG TGTTACAGAA GGAGCGTGGT CACATCTGAA 
TATGGCCCAA TTCTTGACAG CAATCAGCCC CACTCCTTAA ACTCTGATGG CACAGATTTA 
ATAGATGAGG CGTCGTGCTG TGAGACTGTG GGCGAGCGTT TCGAAAACTC TCTGCGTCTG 
GTGTTCAGCC GCTGGGGCTC TCTGTGCGTC CGGCAGCCGC TCACCATCAT CTTGAGCAGT 
CTGGTGCTGA TCTGCATCTG CTCAGCCGGA CTCTCCTACA TGCGCATCAC CACTAACCCT 
GTGGAGCTCT GGTCTGCTCC CAGCAGCAGA GCACGGCAGG AGAAAAACTA CTTCGACCAG 
CACTTCGGGC CCTTCTTCCG CACCGAGCAG CTCATCATCA CCACTCCATG GACCGAGGAG 
GGAGGTTTCT CCACCATCAC AGGAGACATC ATTCCCTTCT CCCCCATTCT GAACCTATCA 
CTGCTGCATC AGGTGCTGGA TCTGCAGTTG GAAATCGAGA ATCTGATCGC TGAATATAAA 
GGAGAGAATG TGACTCTGAA AGACATCTGT GTTTCACCTT TGTCTCCTTA CAATGACAAC 
TGCACCATCC TGAGTGTGTT AAACTACTTC CAGAACAGCC ATGAAGTCCT GGATCATGAG 
TTTCAAGACG AATTCTTCCT CTACAATGAC TACCACACTC ATCTGCTCTA CTGTGCCAGT 
TCCCCGACCT CTCTGGATGA CACGAGCCGT TTGCATGACC CCTGCATGGG CACGTTCGGA 
GGTCCAGTTT TCCCCTGGCT TGTTCTTGGA GGATATGAGG ACTCCGCCTA CAACAATGCC 
ACCGCTCTGG TGATCACTTT TCCTGTCACC AACTACCTGA ATGACACTGA AAAACTAGGC 
AAAGCTTTGG CATGGGAGAA AGAGTTTATT AGGTTTATGA AGAATTACGA GAACCCGAAC 
CTCACGGTGT CCTTCAGCTC CGAGCGCAGT ATCGAGGACG AGATTGACCG CGAGAGCAAC 
AGTGATGTTT CCACCATCGT GATCAGCTAC ATCATCATGT TTGTCTACAT CTCAGTGGCT 
TTGGGCCGCA TCAACAGCTG CAGGACACTG CTGGTGGACT CTAAGATCTC TCTGGGCATC 
GCTGGGATCC TGATCGTGTT GAGTTCAGTG GCGTGTTCGC TGGGGATCTT CAGCTACATT 
GGGATCCCGC TCACGCTTAT CGTCATTGAG GTCATTCCCT TCCTCGTACT GGCCGTAGGA 
GTGGACAACA TCTTTATCAT TGTGCAGACC TATCAGAGAG ACGAGCGAAT GCCAGAGGAG 
GAGCTTCATC AGCAGATTGG CCGAATCCTC GGAGATGTGG CTCCCAGCAT GTTCCTCTCT 
TCCTTCTCTG AGACCGTGGC CTTCTTTTTA GGTGCTCTGT CCACCATGCC TGCGGTGAGG 
ACGTTCTCTC TGTTCGCTGG TCTGGCCATC TTTATCGACT TCCTCCTGCA GATCAGCTGC 
TTTGTGTCTT TATTGGGCCT CGACATCAAG AGACAAGAGG CCAATCGGAT GGATATATTG 
TGCTGTGTGA AGTTGTCTGA TGGACAAGAA GAAAAATCTG AAGGTTGGCT CTTCCGCTTC 
TTCAAGAAAA TATACGCTCC TTTCATCCTG AAGGACTGGG TTCGCCCTCT GGTGGTTGCT 
GTGTTTGTGG GAATGCTGTC CTTCAGCATT GCCGTTGTTA ATAAAGTGGA GATTGGACTG 
GAGCAGACGC TATCCATGCC TGATGACTCA TATGTGCTGA ATTACTTTGG GAATCTGAGC 
AAGTATCTAC ACACCGGGCC GCCGGTTTAC TTCGTGGTTG AAGATGGCCA TGATTATAAG 
ACGTTTGAGG GCCAGAACGC AGTGTGTGGT GGAGTCGGCT GCAACAACGA CTCTCTGGTC 
CAGCAGATTT ACACCGCTTC CCTCATGAGC AACTACACAA GGATCAGTAA TGTACCCTCG 
TCCTGGCTGG ATGACTACTT TGACTGGGTG AAGCCGCAGT CGACCTGCTG CAGATATTAC 
AACAGCACAG GAGCTTTCTG TAACGCTTCA GTGGTGGACA AGAGCTGCGT TCACTGCAGA 
CCAATGACCT CTAGTGGGAA ACAGAGACCG AACGGCACTG AGTTCATGCA CTTCCTCCCC 
ATGTTTCTCT CTGACAACCC CAATATCAAA TGTGGAAAAG GTGGTCATGC AGCGTACGGC 
ACTGCCGTGG ATCTCAAAGA CAACAACACA GATGTGGGCG CCACATACTT CATGAGTTAC 
CACACCATCC TGAAAAACTC CTCCGACTTT ATTAATGCCA TGAAGATGGC CAGAGAGCTT 
ACAGACAACA TCACACAGAC CCTCAGCACG CATGACAAGA GCTACAAAGT CTTCCCTTAC 
AGTGTGTTTT ACGTCTTCTA CGAGCAGTAT CTGACCATCG TCGATGACAC CGCGCTGAAC 
CTCGGCGTGT CTCTGTCCGC CATCTTCATT GTGACGGCAG TTCTGCTGGG CTTTGAGCTC 
TGGTCGGCGG TGCTCGTCTG CTTCACCATC GCTATGATCC TCATCAACAT GTTCGGGGTC 
ATGTGGCTCT GGAGCATCAG CCTCAACGCT GTCTCATTGG TCAACTTGGT CATGAGTTGT 
GGGATATCAG TGGAGTTCTG CAGTCATATA GTGAGGGCTT TCTCCATCAG CACCAGGAGC 
TCCCGGGTGG AGCGGGCAGA GGAGGCGTTG GCACATATGG GCAGCTCCGT CTTCAGTGGA 
ATCACCCTCA CCAAGTTTGG CGGGATCCTG ATTCTCGCTC TGTCCAAATC GCAGATCTTC 
CAGATTTTCT ACTTCCGCAT GTATCTGGCA ATCGTGCTTC TGGGAGCCGC TCACGGCCTC 
ATCTTCCTGC CTGTCTTACT CAGTTATGCA GGTAAATCAC TATCTCCGAG AACATTCATG 
ATTTGTCTTG AAGCAGTAGT TCATTTAAAA CATTTAAAGA CAAGGCATCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163715.1
CDS	1..3834
Translation	MLLLGRNHIFRLLLATVLLSHWVHGQHCIWYGECGNSPNVPEKKLNCNYTGPAVPLPNEGQELLQELCPRLVYADNRVCCDTQQLNTLKSNIQIPLQYLSRCPACFFNFMTLFCELTCSPRQSQFISVKDFLKEKNQTSVGNVTYYITQTFADAMFNACRDVQAPSSNIKALGLLCGRDASVCTPQIWIQYMFSISNGQVPFGIEPIFTDVPVQGMTPMNNRTFNCSQSLDDGSEPCSCQDCSEVCGPTPVPPPIPPPWIILGLDAMSFIMWCSYIAFLLIFFGVVLGAWCYRRSVVTSEYGPILDSNQPHSLNSDGTDLIDEASCCETVGERFENSLRLVFSRWGSLCVRQPLTIILSSLVLICICSAGLSYMRITTNPVELWSAPSSRARQEKNYFDQHFGPFFRTEQLIITTPWTEEGGFSTITGDIIPFSPILNLSLLHQVLDLQLEIENLIAEYKGENVTLKDICVSPLSPYNDNCTILSVLNYFQNSHEVLDHEFQDEFFLYNDYHTHLLYCASSPTSLDDTSRLHDPCMGTFGGPVFPWLVLGGYEDSAYNNATALVITFPVTNYLNDTEKLGKALAWEKEFIRFMKNYENPNLTVSFSSERSIEDEIDRESNSDVSTIVISYIIMFVYISVALGRINSCRTLLVDSKISLGIAGILIVLSSVACSLGIFSYIGIPLTLIVIEVIPFLVLAVGVDNIFIIVQTYQRDERMPEEELHQQIGRILGDVAPSMFLSSFSETVAFFLGALSTMPAVRTFSLFAGLAIFIDFLLQISCFVSLLGLDIKRQEANRMDILCCVKLSDGQEEKSEGWLFRFFKKIYAPFILKDWVRPLVVAVFVGMLSFSIAVVNKVEIGLEQTLSMPDDSYVLNYFGNLSKYLHTGPPVYFVVEDGHDYKTFEGQNAVCGGVGCNNDSLVQQIYTASLMSNYTRISNVPSSWLDDYFDWVKPQSTCCRYYNSTGAFCNASVVDKSCVHCRPMTSSGKQRPNGTEFMHFLPMFLSDNPNIKCGKGGHAAYGTAVDLKDNNTDVGATYFMSYHTILKNSSDFINAMKMARELTDNITQTLSTHDKSYKVFPYSVFYVFYEQYLTIVDDTALNLGVSLSAIFIVTAVLLGFELWSAVLVCFTIAMILINMFGVMWLWSISLNAVSLVNLVMSCGISVEFCSHIVRAFSISTRSSRVERAEEALAHMGSSVFSGITLTKFGGILILALSKSQIFQIFYFRMYLAIVLLGAAHGLIFLPVLLSYAGKSLSPRTFMICLEAVVHLKHLKTRHH

Target ORF information:

RefSeq Version	XM_005163658.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Niemann-Pick disease, type C1 (npc1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163658.3

ORF Insert Sequence:

ATGCTTCTGC TGGGGAGAAA CCACATTTTC CGTCTTTTAT TGGCCACAGT TTTATTATCA 
CATTGGGTGC ATGGCCAGCA CTGTATCTGG TACGGCGAAT GTGGGAACTC CCCAAACGTC 
CCAGAAAAGA AGTTGAACTG CAATTACACC GGTCCTGCTG TGCCTCTGCC TAATGAAGGA 
CAGGAGCTGC TGCAGGAGCT GTGTCCGAGA CTGGTCTACG CAGACAACAG AGTTTGCTGC 
GATACACAAC AGCTCAACAC ACTAAAAAGC AACATCCAGA TCCCCCTTCA GTACCTGTCC 
AGGTGTCCAG CATGTTTCTT CAACTTCATG ACCCTGTTCT GCGAGCTCAC CTGCAGCCCC 
AGACAGAGCC AGTTTATCAG CGTAAAAGAC TTCCTTAAAG AGAAAAACCA GACCAGTGTG 
GGGAATGTGA CCTATTATAT CACCCAGACC TTTGCCGATG CCATGTTTAA CGCCTGCAGA 
GATGTCCAGG CTCCATCCAG CAACATCAAA GCTCTCGGTC TGTTGTGCGG CAGAGACGCC 
AGTGTGTGTA CTCCTCAAAT TTGGATCCAG TACATGTTTA GTATCAGCAA CGGACAGGTT 
CCCTTTGGCA TCGAACCGAT TTTCACAGAT GTCCCTGTGC AGGGCATGAC CCCAATGAAT 
AACAGGACGT TTAACTGCTC TCAGTCTCTG GATGACGGCT CGGAACCCTG CTCGTGTCAG 
GACTGCAGTG AAGTGTGCGG GCCCACGCCT GTGCCCCCTC CCATCCCTCC TCCCTGGATT 
ATCCTCGGCC TGGATGCCAT GAGCTTCATC ATGTGGTGCT CTTACATCGC TTTCCTCCTC 
ATCTTCTTCG GAGTGGTTCT GGGAGCTTGG TGTTACAGAA GGAGCGTGGT CACATCTGAA 
TATGGCCCAA TTCTTGACAG CAATCAGCCC CACTCCTTAA ACTCTGATGG CACAGATTTA 
ATAGATGAGG CGTCGTGCTG TGAGACTGTG GGCGAGCGTT TCGAAAACTC TCTGCGTCTG 
GTGTTCAGCC GCTGGGGCTC TCTGTGCGTC CGGCAGCCGC TCACCATCAT CTTGAGCAGT 
CTGGTGCTGA TCTGCATCTG CTCAGCCGGA CTCTCCTACA TGCGCATCAC CACTAACCCT 
GTGGAGCTCT GGTCTGCTCC CAGCAGCAGA GCACGGCAGG AGAAAAACTA CTTCGACCAG 
CACTTCGGGC CCTTCTTCCG CACCGAGCAG CTCATCATCA CCACTCCATG GACCGAGGAG 
GGAGGTTTCT CCACCATCAC AGGAGACATC ATTCCCTTCT CCCCCATTCT GAACCTATCA 
CTGCTGCATC AGGTGCTGGA TCTGCAGTTG GAAATCGAGA ATCTGATCGC TGAATATAAA 
GGAGAGAATG TGACTCTGAA AGACATCTGT GTTTCACCTT TGTCTCCTTA CAATGACAAC 
TGCACCATCC TGAGTGTGTT AAACTACTTC CAGAACAGCC ATGAAGTCCT GGATCATGAG 
TTTCAAGACG AATTCTTCCT CTACAATGAC TACCACACTC ATCTGCTCTA CTGTGCCAGT 
TCCCCGACCT CTCTGGATGA CACGAGCCGT TTGCATGACC CCTGCATGGG CACGTTCGGA 
GGTCCAGTTT TCCCCTGGCT TGTTCTTGGA GGATATGAGG ACTCCGCCTA CAACAATGCC 
ACCGCTCTGG TGATCACTTT TCCTGTCACC AACTACCTGA ATGACACTGA AAAACTAGGC 
AAAGCTTTGG CATGGGAGAA AGAGTTTATT AGGTTTATGA AGAATTACGA GAACCCGAAC 
CTCACGGTGT CCTTCAGCTC CGAGCGCAGT ATCGAGGACG AGATTGACCG CGAGAGCAAC 
AGTGATGTTT CCACCATCGT GATCAGCTAC ATCATCATGT TTGTCTACAT CTCAGTGGCT 
TTGGGCCGCA TCAACAGCTG CAGGACACTG CTGGTGGACT CTAAGATCTC TCTGGGCATC 
GCTGGGATCC TGATCGTGTT GAGTTCAGTG GCGTGTTCGC TGGGGATCTT CAGCTACATT 
GGGATCCCGC TCACGCTTAT CGTCATTGAG GTCATTCCCT TCCTCGTACT GGCCGTAGGA 
GTGGACAACA TCTTTATCAT TGTGCAGACC TATCAGAGAG ACGAGCGAAT GCCAGAGGAG 
GAGCTTCATC AGCAGATTGG CCGAATCCTC GGAGATGTGG CTCCCAGCAT GTTCCTCTCT 
TCCTTCTCTG AGACCGTGGC CTTCTTTTTA GGTGCTCTGT CCACCATGCC TGCGGTGAGG 
ACGTTCTCTC TGTTCGCTGG TCTGGCCATC TTTATCGACT TCCTCCTGCA GATCAGCTGC 
TTTGTGTCTT TATTGGGCCT CGACATCAAG AGACAAGAGG CCAATCGGAT GGATATATTG 
TGCTGTGTGA AGTTGTCTGA TGGACAAGAA GAAAAATCTG AAGGTTGGCT CTTCCGCTTC 
TTCAAGAAAA TATACGCTCC TTTCATCCTG AAGGACTGGG TTCGCCCTCT GGTGGTTGCT 
GTGTTTGTGG GAATGCTGTC CTTCAGCATT GCCGTTGTTA ATAAAGTGGA GATTGGACTG 
GAGCAGACGC TATCCATGCC TGATGACTCA TATGTGCTGA ATTACTTTGG GAATCTGAGC 
AAGTATCTAC ACACCGGGCC GCCGGTTTAC TTCGTGGTTG AAGATGGCCA TGATTATAAG 
ACGTTTGAGG GCCAGAACGC AGTGTGTGGT GGAGTCGGCT GCAACAACGA CTCTCTGGTC 
CAGCAGATTT ACACCGCTTC CCTCATGAGC AACTACACAA GGATCAGTAA TGTACCCTCG 
TCCTGGCTGG ATGACTACTT TGACTGGGTG AAGCCGCAGT CGACCTGCTG CAGATATTAC 
AACAGCACAG GAGCTTTCTG TAACGCTTCA GTGGTGGACA AGAGCTGCGT TCACTGCAGA 
CCAATGACCT CTAGTGGGAA ACAGAGACCG AACGGCACTG AGTTCATGCA CTTCCTCCCC 
ATGTTTCTCT CTGACAACCC CAATATCAAA TGTGGAAAAG GTGGTCATGC AGCGTACGGC 
ACTGCCGTGG ATCTCAAAGA CAACAACACA GATGTGGGCG CCACATACTT CATGAGTTAC 
CACACCATCC TGAAAAACTC CTCCGACTTT ATTAATGCCA TGAAGATGGC CAGAGAGCTT 
ACAGACAACA TCACACAGAC CCTCAGCACG CATGACAAGA GCTACAAAGT CTTCCCTTAC 
AGTGTGTTTT ACGTCTTCTA CGAGCAGTAT CTGACCATCG TCGATGACAC CGCGCTGAAC 
CTCGGCGTGT CTCTGTCCGC CATCTTCATT GTGACGGCAG TTCTGCTGGG CTTTGAGCTC 
TGGTCGGCGG TGCTCGTCTG CTTCACCATC GCTATGATCC TCATCAACAT GTTCGGGGTC 
ATGTGGCTCT GGAGCATCAG CCTCAACGCT GTCTCATTGG TCAACTTGGT CATGAGTTGT 
GGGATATCAG TGGAGTTCTG CAGTCATATA GTGAGGGCTT TCTCCATCAG CACCAGGAGC 
TCCCGGGTGG AGCGGGCAGA GGAGGCGTTG GCACATATGG GCAGCTCCGT CTTCAGTGGA 
ATCACCCTCA CCAAGTTTGG CGGGATCCTG ATTCTCGCTC TGTCCAAATC GCAGATCTTC 
CAGATTTTCT ACTTCCGCAT GTATCTGGCA ATCGTGCTTC TGGGAGCCGC TCACGGCCTC 
ATCTTCCTGC CTGTCTTACT CAGTTATGCA GGTAAATCAC TATCTCCGAG AACATTCATG 
ATTTGTCTTG AAGCAGTAGT TCATTTAAAA CATTTAAAGA CAAGGCATCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

npc1 ( XM_005163658.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005163715.1 Danio rerio Niemann-Pick disease, type C1 (npc1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa06625
Clone ID Related Accession (Same CDS sequence)	XM_005163658.2 , XM_005163658.3 , XM_005163658.4
Accession Version	XM_005163658.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	Niemann-Pick C1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005163658.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCTGC TGGGGAGAAA CCACATTTTC CGTCTTTTAT TGGCCACAGT TTTATTATCA 
CATTGGGTGC ATGGCCAGCA CTGTATCTGG TACGGCGAAT GTGGGAACTC CCCAAACGTC 
CCAGAAAAGA AGTTGAACTG CAATTACACC GGTCCTGCTG TGCCTCTGCC TAATGAAGGA 
CAGGAGCTGC TGCAGGAGCT GTGTCCGAGA CTGGTCTACG CAGACAACAG AGTTTGCTGC 
GATACACAAC AGCTCAACAC ACTAAAAAGC AACATCCAGA TCCCCCTTCA GTACCTGTCC 
AGGTGTCCAG CATGTTTCTT CAACTTCATG ACCCTGTTCT GCGAGCTCAC CTGCAGCCCC 
AGACAGAGCC AGTTTATCAG CGTAAAAGAC TTCCTTAAAG AGAAAAACCA GACCAGTGTG 
GGGAATGTGA CCTATTATAT CACCCAGACC TTTGCCGATG CCATGTTTAA CGCCTGCAGA 
GATGTCCAGG CTCCATCCAG CAACATCAAA GCTCTCGGTC TGTTGTGCGG CAGAGACGCC 
AGTGTGTGTA CTCCTCAAAT TTGGATCCAG TACATGTTTA GTATCAGCAA CGGACAGGTT 
CCCTTTGGCA TCGAACCGAT TTTCACAGAT GTCCCTGTGC AGGGCATGAC CCCAATGAAT 
AACAGGACGT TTAACTGCTC TCAGTCTCTG GATGACGGCT CGGAACCCTG CTCGTGTCAG 
GACTGCAGTG AAGTGTGCGG GCCCACGCCT GTGCCCCCTC CCATCCCTCC TCCCTGGATT 
ATCCTCGGCC TGGATGCCAT GAGCTTCATC ATGTGGTGCT CTTACATCGC TTTCCTCCTC 
ATCTTCTTCG GAGTGGTTCT GGGAGCTTGG TGTTACAGAA GGAGCGTGGT CACATCTGAA 
TATGGCCCAA TTCTTGACAG CAATCAGCCC CACTCCTTAA ACTCTGATGG CACAGATTTA 
ATAGATGAGG CGTCGTGCTG TGAGACTGTG GGCGAGCGTT TCGAAAACTC TCTGCGTCTG 
GTGTTCAGCC GCTGGGGCTC TCTGTGCGTC CGGCAGCCGC TCACCATCAT CTTGAGCAGT 
CTGGTGCTGA TCTGCATCTG CTCAGCCGGA CTCTCCTACA TGCGCATCAC CACTAACCCT 
GTGGAGCTCT GGTCTGCTCC CAGCAGCAGA GCACGGCAGG AGAAAAACTA CTTCGACCAG 
CACTTCGGGC CCTTCTTCCG CACCGAGCAG CTCATCATCA CCACTCCATG GACCGAGGAG 
GGAGGTTTCT CCACCATCAC AGGAGACATC ATTCCCTTCT CCCCCATTCT GAACCTATCA 
CTGCTGCATC AGGTGCTGGA TCTGCAGTTG GAAATCGAGA ATCTGATCGC TGAATATAAA 
GGAGAGAATG TGACTCTGAA AGACATCTGT GTTTCACCTT TGTCTCCTTA CAATGACAAC 
TGCACCATCC TGAGTGTGTT AAACTACTTC CAGAACAGCC ATGAAGTCCT GGATCATGAG 
TTTCAAGACG AATTCTTCCT CTACAATGAC TACCACACTC ATCTGCTCTA CTGTGCCAGT 
TCCCCGACCT CTCTGGATGA CACGAGCCGT TTGCATGACC CCTGCATGGG CACGTTCGGA 
GGTCCAGTTT TCCCCTGGCT TGTTCTTGGA GGATATGAGG ACTCCGCCTA CAACAATGCC 
ACCGCTCTGG TGATCACTTT TCCTGTCACC AACTACCTGA ATGACACTGA AAAACTAGGC 
AAAGCTTTGG CATGGGAGAA AGAGTTTATT AGGTTTATGA AGAATTACGA GAACCCGAAC 
CTCACGGTGT CCTTCAGCTC CGAGCGCAGT ATCGAGGACG AGATTGACCG CGAGAGCAAC 
AGTGATGTTT CCACCATCGT GATCAGCTAC ATCATCATGT TTGTCTACAT CTCAGTGGCT 
TTGGGCCGCA TCAACAGCTG CAGGACACTG CTGGTGGACT CTAAGATCTC TCTGGGCATC 
GCTGGGATCC TGATCGTGTT GAGTTCAGTG GCGTGTTCGC TGGGGATCTT CAGCTACATT 
GGGATCCCGC TCACGCTTAT CGTCATTGAG GTCATTCCCT TCCTCGTACT GGCCGTAGGA 
GTGGACAACA TCTTTATCAT TGTGCAGACC TATCAGAGAG ACGAGCGAAT GCCAGAGGAG 
GAGCTTCATC AGCAGATTGG CCGAATCCTC GGAGATGTGG CTCCCAGCAT GTTCCTCTCT 
TCCTTCTCTG AGACCGTGGC CTTCTTTTTA GGTGCTCTGT CCACCATGCC TGCGGTGAGG 
ACGTTCTCTC TGTTCGCTGG TCTGGCCATC TTTATCGACT TCCTCCTGCA GATCAGCTGC 
TTTGTGTCTT TATTGGGCCT CGACATCAAG AGACAAGAGG CCAATCGGAT GGATATATTG 
TGCTGTGTGA AGTTGTCTGA TGGACAAGAA GAAAAATCTG AAGGTTGGCT CTTCCGCTTC 
TTCAAGAAAA TATACGCTCC TTTCATCCTG AAGGACTGGG TTCGCCCTCT GGTGGTTGCT 
GTGTTTGTGG GAATGCTGTC CTTCAGCATT GCCGTTGTTA ATAAAGTGGA GATTGGACTG 
GAGCAGACGC TATCCATGCC TGATGACTCA TATGTGCTGA ATTACTTTGG GAATCTGAGC 
AAGTATCTAC ACACCGGGCC GCCGGTTTAC TTCGTGGTTG AAGATGGCCA TGATTATAAG 
ACGTTTGAGG GCCAGAACGC AGTGTGTGGT GGAGTCGGCT GCAACAACGA CTCTCTGGTC 
CAGCAGATTT ACACCGCTTC CCTCATGAGC AACTACACAA GGATCAGTAA TGTACCCTCG 
TCCTGGCTGG ATGACTACTT TGACTGGGTG AAGCCGCAGT CGACCTGCTG CAGATATTAC 
AACAGCACAG GAGCTTTCTG TAACGCTTCA GTGGTGGACA AGAGCTGCGT TCACTGCAGA 
CCAATGACCT CTAGTGGGAA ACAGAGACCG AACGGCACTG AGTTCATGCA CTTCCTCCCC 
ATGTTTCTCT CTGACAACCC CAATATCAAA TGTGGAAAAG GTGGTCATGC AGCGTACGGC 
ACTGCCGTGG ATCTCAAAGA CAACAACACA GATGTGGGCG CCACATACTT CATGAGTTAC 
CACACCATCC TGAAAAACTC CTCCGACTTT ATTAATGCCA TGAAGATGGC CAGAGAGCTT 
ACAGACAACA TCACACAGAC CCTCAGCACG CATGACAAGA GCTACAAAGT CTTCCCTTAC 
AGTGTGTTTT ACGTCTTCTA CGAGCAGTAT CTGACCATCG TCGATGACAC CGCGCTGAAC 
CTCGGCGTGT CTCTGTCCGC CATCTTCATT GTGACGGCAG TTCTGCTGGG CTTTGAGCTC 
TGGTCGGCGG TGCTCGTCTG CTTCACCATC GCTATGATCC TCATCAACAT GTTCGGGGTC 
ATGTGGCTCT GGAGCATCAG CCTCAACGCT GTCTCATTGG TCAACTTGGT CATGAGTTGT 
GGGATATCAG TGGAGTTCTG CAGTCATATA GTGAGGGCTT TCTCCATCAG CACCAGGAGC 
TCCCGGGTGG AGCGGGCAGA GGAGGCGTTG GCACATATGG GCAGCTCCGT CTTCAGTGGA 
ATCACCCTCA CCAAGTTTGG CGGGATCCTG ATTCTCGCTC TGTCCAAATC GCAGATCTTC 
CAGATTTTCT ACTTCCGCAT GTATCTGGCA ATCGTGCTTC TGGGAGCCGC TCACGGCCTC 
ATCTTCCTGC CTGTCTTACT CAGTTATGCA GGTAAATCAC TATCTCCGAG AACATTCATG 
ATTTGTCTTG AAGCAGTAGT TCATTTAAAA CATTTAAAGA CAAGGCATCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163715.1
CDS	88..3921
Translation	MLLLGRNHIFRLLLATVLLSHWVHGQHCIWYGECGNSPNVPEKKLNCNYTGPAVPLPNEGQELLQELCPRLVYADNRVCCDTQQLNTLKSNIQIPLQYLSRCPACFFNFMTLFCELTCSPRQSQFISVKDFLKEKNQTSVGNVTYYITQTFADAMFNACRDVQAPSSNIKALGLLCGRDASVCTPQIWIQYMFSISNGQVPFGIEPIFTDVPVQGMTPMNNRTFNCSQSLDDGSEPCSCQDCSEVCGPTPVPPPIPPPWIILGLDAMSFIMWCSYIAFLLIFFGVVLGAWCYRRSVVTSEYGPILDSNQPHSLNSDGTDLIDEASCCETVGERFENSLRLVFSRWGSLCVRQPLTIILSSLVLICICSAGLSYMRITTNPVELWSAPSSRARQEKNYFDQHFGPFFRTEQLIITTPWTEEGGFSTITGDIIPFSPILNLSLLHQVLDLQLEIENLIAEYKGENVTLKDICVSPLSPYNDNCTILSVLNYFQNSHEVLDHEFQDEFFLYNDYHTHLLYCASSPTSLDDTSRLHDPCMGTFGGPVFPWLVLGGYEDSAYNNATALVITFPVTNYLNDTEKLGKALAWEKEFIRFMKNYENPNLTVSFSSERSIEDEIDRESNSDVSTIVISYIIMFVYISVALGRINSCRTLLVDSKISLGIAGILIVLSSVACSLGIFSYIGIPLTLIVIEVIPFLVLAVGVDNIFIIVQTYQRDERMPEEELHQQIGRILGDVAPSMFLSSFSETVAFFLGALSTMPAVRTFSLFAGLAIFIDFLLQISCFVSLLGLDIKRQEANRMDILCCVKLSDGQEEKSEGWLFRFFKKIYAPFILKDWVRPLVVAVFVGMLSFSIAVVNKVEIGLEQTLSMPDDSYVLNYFGNLSKYLHTGPPVYFVVEDGHDYKTFEGQNAVCGGVGCNNDSLVQQIYTASLMSNYTRISNVPSSWLDDYFDWVKPQSTCCRYYNSTGAFCNASVVDKSCVHCRPMTSSGKQRPNGTEFMHFLPMFLSDNPNIKCGKGGHAAYGTAVDLKDNNTDVGATYFMSYHTILKNSSDFINAMKMARELTDNITQTLSTHDKSYKVFPYSVFYVFYEQYLTIVDDTALNLGVSLSAIFIVTAVLLGFELWSAVLVCFTIAMILINMFGVMWLWSISLNAVSLVNLVMSCGISVEFCSHIVRAFSISTRSSRVERAEEALAHMGSSVFSGITLTKFGGILILALSKSQIFQIFYFRMYLAIVLLGAAHGLIFLPVLLSYAGKSLSPRTFMICLEAVVHLKHLKTRHH

Target ORF information:

RefSeq Version	XM_005163658.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Niemann-Pick disease, type C1 (npc1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163658.4

ORF Insert Sequence:

ATGCTTCTGC TGGGGAGAAA CCACATTTTC CGTCTTTTAT TGGCCACAGT TTTATTATCA 
CATTGGGTGC ATGGCCAGCA CTGTATCTGG TACGGCGAAT GTGGGAACTC CCCAAACGTC 
CCAGAAAAGA AGTTGAACTG CAATTACACC GGTCCTGCTG TGCCTCTGCC TAATGAAGGA 
CAGGAGCTGC TGCAGGAGCT GTGTCCGAGA CTGGTCTACG CAGACAACAG AGTTTGCTGC 
GATACACAAC AGCTCAACAC ACTAAAAAGC AACATCCAGA TCCCCCTTCA GTACCTGTCC 
AGGTGTCCAG CATGTTTCTT CAACTTCATG ACCCTGTTCT GCGAGCTCAC CTGCAGCCCC 
AGACAGAGCC AGTTTATCAG CGTAAAAGAC TTCCTTAAAG AGAAAAACCA GACCAGTGTG 
GGGAATGTGA CCTATTATAT CACCCAGACC TTTGCCGATG CCATGTTTAA CGCCTGCAGA 
GATGTCCAGG CTCCATCCAG CAACATCAAA GCTCTCGGTC TGTTGTGCGG CAGAGACGCC 
AGTGTGTGTA CTCCTCAAAT TTGGATCCAG TACATGTTTA GTATCAGCAA CGGACAGGTT 
CCCTTTGGCA TCGAACCGAT TTTCACAGAT GTCCCTGTGC AGGGCATGAC CCCAATGAAT 
AACAGGACGT TTAACTGCTC TCAGTCTCTG GATGACGGCT CGGAACCCTG CTCGTGTCAG 
GACTGCAGTG AAGTGTGCGG GCCCACGCCT GTGCCCCCTC CCATCCCTCC TCCCTGGATT 
ATCCTCGGCC TGGATGCCAT GAGCTTCATC ATGTGGTGCT CTTACATCGC TTTCCTCCTC 
ATCTTCTTCG GAGTGGTTCT GGGAGCTTGG TGTTACAGAA GGAGCGTGGT CACATCTGAA 
TATGGCCCAA TTCTTGACAG CAATCAGCCC CACTCCTTAA ACTCTGATGG CACAGATTTA 
ATAGATGAGG CGTCGTGCTG TGAGACTGTG GGCGAGCGTT TCGAAAACTC TCTGCGTCTG 
GTGTTCAGCC GCTGGGGCTC TCTGTGCGTC CGGCAGCCGC TCACCATCAT CTTGAGCAGT 
CTGGTGCTGA TCTGCATCTG CTCAGCCGGA CTCTCCTACA TGCGCATCAC CACTAACCCT 
GTGGAGCTCT GGTCTGCTCC CAGCAGCAGA GCACGGCAGG AGAAAAACTA CTTCGACCAG 
CACTTCGGGC CCTTCTTCCG CACCGAGCAG CTCATCATCA CCACTCCATG GACCGAGGAG 
GGAGGTTTCT CCACCATCAC AGGAGACATC ATTCCCTTCT CCCCCATTCT GAACCTATCA 
CTGCTGCATC AGGTGCTGGA TCTGCAGTTG GAAATCGAGA ATCTGATCGC TGAATATAAA 
GGAGAGAATG TGACTCTGAA AGACATCTGT GTTTCACCTT TGTCTCCTTA CAATGACAAC 
TGCACCATCC TGAGTGTGTT AAACTACTTC CAGAACAGCC ATGAAGTCCT GGATCATGAG 
TTTCAAGACG AATTCTTCCT CTACAATGAC TACCACACTC ATCTGCTCTA CTGTGCCAGT 
TCCCCGACCT CTCTGGATGA CACGAGCCGT TTGCATGACC CCTGCATGGG CACGTTCGGA 
GGTCCAGTTT TCCCCTGGCT TGTTCTTGGA GGATATGAGG ACTCCGCCTA CAACAATGCC 
ACCGCTCTGG TGATCACTTT TCCTGTCACC AACTACCTGA ATGACACTGA AAAACTAGGC 
AAAGCTTTGG CATGGGAGAA AGAGTTTATT AGGTTTATGA AGAATTACGA GAACCCGAAC 
CTCACGGTGT CCTTCAGCTC CGAGCGCAGT ATCGAGGACG AGATTGACCG CGAGAGCAAC 
AGTGATGTTT CCACCATCGT GATCAGCTAC ATCATCATGT TTGTCTACAT CTCAGTGGCT 
TTGGGCCGCA TCAACAGCTG CAGGACACTG CTGGTGGACT CTAAGATCTC TCTGGGCATC 
GCTGGGATCC TGATCGTGTT GAGTTCAGTG GCGTGTTCGC TGGGGATCTT CAGCTACATT 
GGGATCCCGC TCACGCTTAT CGTCATTGAG GTCATTCCCT TCCTCGTACT GGCCGTAGGA 
GTGGACAACA TCTTTATCAT TGTGCAGACC TATCAGAGAG ACGAGCGAAT GCCAGAGGAG 
GAGCTTCATC AGCAGATTGG CCGAATCCTC GGAGATGTGG CTCCCAGCAT GTTCCTCTCT 
TCCTTCTCTG AGACCGTGGC CTTCTTTTTA GGTGCTCTGT CCACCATGCC TGCGGTGAGG 
ACGTTCTCTC TGTTCGCTGG TCTGGCCATC TTTATCGACT TCCTCCTGCA GATCAGCTGC 
TTTGTGTCTT TATTGGGCCT CGACATCAAG AGACAAGAGG CCAATCGGAT GGATATATTG 
TGCTGTGTGA AGTTGTCTGA TGGACAAGAA GAAAAATCTG AAGGTTGGCT CTTCCGCTTC 
TTCAAGAAAA TATACGCTCC TTTCATCCTG AAGGACTGGG TTCGCCCTCT GGTGGTTGCT 
GTGTTTGTGG GAATGCTGTC CTTCAGCATT GCCGTTGTTA ATAAAGTGGA GATTGGACTG 
GAGCAGACGC TATCCATGCC TGATGACTCA TATGTGCTGA ATTACTTTGG GAATCTGAGC 
AAGTATCTAC ACACCGGGCC GCCGGTTTAC TTCGTGGTTG AAGATGGCCA TGATTATAAG 
ACGTTTGAGG GCCAGAACGC AGTGTGTGGT GGAGTCGGCT GCAACAACGA CTCTCTGGTC 
CAGCAGATTT ACACCGCTTC CCTCATGAGC AACTACACAA GGATCAGTAA TGTACCCTCG 
TCCTGGCTGG ATGACTACTT TGACTGGGTG AAGCCGCAGT CGACCTGCTG CAGATATTAC 
AACAGCACAG GAGCTTTCTG TAACGCTTCA GTGGTGGACA AGAGCTGCGT TCACTGCAGA 
CCAATGACCT CTAGTGGGAA ACAGAGACCG AACGGCACTG AGTTCATGCA CTTCCTCCCC 
ATGTTTCTCT CTGACAACCC CAATATCAAA TGTGGAAAAG GTGGTCATGC AGCGTACGGC 
ACTGCCGTGG ATCTCAAAGA CAACAACACA GATGTGGGCG CCACATACTT CATGAGTTAC 
CACACCATCC TGAAAAACTC CTCCGACTTT ATTAATGCCA TGAAGATGGC CAGAGAGCTT 
ACAGACAACA TCACACAGAC CCTCAGCACG CATGACAAGA GCTACAAAGT CTTCCCTTAC 
AGTGTGTTTT ACGTCTTCTA CGAGCAGTAT CTGACCATCG TCGATGACAC CGCGCTGAAC 
CTCGGCGTGT CTCTGTCCGC CATCTTCATT GTGACGGCAG TTCTGCTGGG CTTTGAGCTC 
TGGTCGGCGG TGCTCGTCTG CTTCACCATC GCTATGATCC TCATCAACAT GTTCGGGGTC 
ATGTGGCTCT GGAGCATCAG CCTCAACGCT GTCTCATTGG TCAACTTGGT CATGAGTTGT 
GGGATATCAG TGGAGTTCTG CAGTCATATA GTGAGGGCTT TCTCCATCAG CACCAGGAGC 
TCCCGGGTGG AGCGGGCAGA GGAGGCGTTG GCACATATGG GCAGCTCCGT CTTCAGTGGA 
ATCACCCTCA CCAAGTTTGG CGGGATCCTG ATTCTCGCTC TGTCCAAATC GCAGATCTTC 
CAGATTTTCT ACTTCCGCAT GTATCTGGCA ATCGTGCTTC TGGGAGCCGC TCACGGCCTC 
ATCTTCCTGC CTGTCTTACT CAGTTATGCA GGTAAATCAC TATCTCCGAG AACATTCATG 
ATTTGTCTTG AAGCAGTAGT TCATTTAAAA CATTTAAAGA CAAGGCATCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

npc1 ( XM_005163659.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005163716.1 Danio rerio Niemann-Pick disease, type C1 (npc1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa06626
Clone ID Related Accession (Same CDS sequence)	XM_005163659.4 , XM_005163659.3
Accession Version	XM_005163659.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3831bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	Niemann-Pick C1 protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005163659.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCTGC TGGGGAGAAA CCACATTTTC CGTCTTTTAT TGGCCACAGT TTTATTATCA 
CATTGGGTGC ATGGCCAGCA CTGTATCTGG TACGGCGAAT GTGGGAACTC CCCAAACGTC 
CCAGAAAAGA AGTTGAACTG CAATTACACC GGTCCTGCTG TGCCTCTGCC TAATGAAGGA 
CAGGAGCTGC TGCAGGAGCT GTGTCCGAGA CTGGTCTACG CAGACAACAG AGTTTGCTGC 
GATACACAAC AGCTCAACAC ACTAAAAAGC AACATCCAGA TCCCCCTTCA GTACCTGTCC 
AGGTGTCCAG CATGTTTCTT CAACTTCATG ACCCTGTTCT GCGAGCTCAC CTGCAGCCCC 
AGACAGAGCC AGTTTATCAG CGTAAAAGAC TTCCTTAAAG AGAAAAACCA GACCAGTGTG 
GGGAATGTGA CCTATTATAT CACCCAGACC TTTGCCGATG CCATGTTTAA CGCCTGCAGA 
GATGTCCAGG CTCCATCCAG CAACATCAAA GCTCTCGGTC TGTTGTGCGG CAGAGACGCC 
AGTGTGTGTA CTCCTCAAAT TTGGATCCAG TACATGTTTA GTATCAGCAA CGGACAGGTT 
CCCTTTGGCA TCGAACCGAT TTTCACAGAT GTCCCTGTGC AGGGCATGAC CCCAATGAAT 
AACAGGACGT TTAACTGCTC TCAGTCTCTG GATGACGGCT CGGAACCCTG CTCGTGTCAG 
GACTGCAGTG AAGTGTGCGG GCCCACGCCT GTGCCCCCTC CCATCCCTCC TCCCTGGATT 
ATCCTCGGCC TGGATGCCAT GAGCTTCATC ATGTGGTGCT CTTACATCGC TTTCCTCCTC 
ATCTTCTTCG GAGTGGTTCT GGGAGCTTGG TGTTACAGAA GGAGCGTGGT CACATCTGAA 
TATGGCCCAA TTCTTGACAG CAATCAGCCC CACTCCTTAA ACTCTGATGG CACAGATTTA 
ATAGATGAGG CGTCGTGCTG TGAGACTGTG GGCGAGCGTT TCGAAAACTC TCTGCGTCTG 
GTGTTCAGCC GCTGGGGCTC TCTGTGCGTC CGGCAGCCGC TCACCATCAT CTTGAGCAGT 
CTGGTGCTGA TCTGCATCTG CTCAGCCGGA CTCTCCTACA TGCGCATCAC CACTAACCCT 
GTGGAGCTCT GGTCTGCTCC CAGCAGCAGA GCACGGCAGG AGAAAAACTA CTTCGACCAG 
CACTTCGGGC CCTTCTTCCG CACCGAGCAG CTCATCATCA CCACTCCATG GACCGAGGAG 
GGAGGTTTCT CCACCATCAC AGGAGACATC ATTCCCTTCT CCCCCATTCT GAACCTATCA 
CTGCTGCATC AGGTGCTGGA TCTGCAGTTG GAAATCGAGA ATCTGATCGC TGAATATAAA 
GGAGAGAATG TGACTCTGAA AGACATCTGT GTTTCACCTT TGTCTCCTTA CAATGACAAC 
TGCACCATCC TGAGTGTGTT AAACTACTTC CAGAACAGCC ATGAAGTCCT GGATCATGAG 
TTTCAAGACG AATTCTTCCT CTACAATGAC TACCACACTC ATCTGCTCTA CTGTGCCAGT 
TCCCCGACCT CTCTGGATGA CACGAGCCGT TTGCATGACC CCTGCATGGG CACGTTCGGA 
GGTCCAGTTT TCCCCTGGCT TGTTCTTGGA GGATATGAGG ACTCCGCCTA CAACAATGCC 
ACCGCTCTGG TGATCACTTT TCCTGTCACC AACTACCTGA ATGACACTGA AAAACTAGGC 
AAAGCTTTGG CATGGGAGAA AGAGTTTATT AGGTTTATGA AGAATTACGA GAACCCGAAC 
CTCACGGTGT CCTTCAGCTC CGAGCGCAGT ATCGAGGACG AGATTGACCG CGAGAGCAAC 
AGTGATGTTT CCACCATCGT GATCAGCTAC ATCATCATGT TTGTCTACAT CTCAGTGGCT 
TTGGGCCGCA TCAACAGCTG CAGGACACTG CTGGTGGACT CTAAGATCTC TCTGGGCATC 
GCTGGGATCC TGATCGTGTT GAGTTCAGTG GCGTGTTCGC TGGGGATCTT CAGCTACATT 
GGGATCCCGC TCACGCTTAT CGTCATTGAG GTCATTCCCT TCCTCGTACT GGCCGTAGGA 
GTGGACAACA TCTTTATCAT TGTGCAGACC TATCAGAGAG ACGAGCGAAT GCCAGAGGAG 
GAGCTTCATC AGCAGATTGG CCGAATCCTC GGAGATGTGG CTCCCAGCAT GTTCCTCTCT 
TCCTTCTCTG AGACCGTGGC CTTCTTTTTA GGTGCTCTGT CCACCATGCC TGCGGTGAGG 
ACGTTCTCTC TGTTCGCTGG TCTGGCCATC TTTATCGACT TCCTCCTGCA GATCAGCTGC 
TTTGTGTCTT TATTGGGCCT CGACATCAAG AGACAAGAGG CCAATCGGAT GGATATATTG 
TGCTGTGTGA AGTTGTCTGA TGGACAAGAA GAAAAATCTG AAGGTTGGCT CTTCCGCTTC 
TTCAAGAAAA TATACGCTCC TTTCATCCTG AAGGACTGGG TTCGCCCTCT GGTGGTTGCT 
GTGTTTGTGG GAATGCTGTC CTTCAGCATT GCCGTTGTTA ATAAAGTGGA GATTGGACTG 
GAGCAGACGC TATCCATGCC TGATGACTCA TATGTGCTGA ATTACTTTGG GAATCTGAGC 
AAGTATCTAC ACACCGGGCC GCCGGTTTAC TTCGTGGTTG AAGATGGCCA TGATTATAAG 
ACGTTTGAGG GCCAGAACGC AGTGTGTGGT GGAGTCGGCT GCAACAACGA CTCTCTGGTC 
CAGCAGATTT ACACCGCTTC CCTCATGAGC AACTACACAA GGATCAGTAA TGTACCCTCG 
TCCTGGCTGG ATGACTACTT TGACTGGGTG AAGCCGCAGT CGACCTGCTG CAGATATTAC 
AACAGCACAG GAGCTTTCTG TAACGCTTCA GTGGTGGACA AGAGCTGCGT TCACTGCAGA 
CCAATGACCT CTAGTGGGAA ACAGAGACCG AACGGCACTG AGTTCATGCA CTTCCTCCCC 
ATGTTTCTCT CTGACAACCC CAATATCAAA TGTGGAAAAG GTGGTCATGC AGCGTACGGC 
ACTGCCGTGG ATCTCAAAGA CAACAACACA GATGTGGGCG CCACATACTT CATGAGTTAC 
CACACCATCC TGAAAAACTC CTCCGACTTT ATTAATGCCA TGAAGATGGC CAGAGAGCTT 
ACAGACAACA TCACACAGAC CCTCAGCACG CATGACAAGA GCTACAAAGT CTTCCCTTAC 
AGTGTGTTTT ACGTCTTCTA CGAGCAGTAT CTGACCATCG TCGATGACAC CGCGCTGAAC 
CTCGGCGTGT CTCTGTCCGC CATCTTCATT GTGACGGCAG TTCTGCTGGG CTTTGAGCTC 
TGGTCGGCGG TGCTCGTCTG CTTCACCATC GCTATGATCC TCATCAACAT GTTCGGGGTC 
ATGTGGCTCT GGAGCATCAG CCTCAACGCT GTCTCATTGG TCAACTTGGT CATGAGTTGT 
GGGATATCAG TGGAGTTCTG CAGTCATATA GTGAGGGCTT TCTCCATCAG CACCAGGAGC 
TCCCGGGTGG AGCGGGCAGA GGAGGCGTTG GCACATATGG GCAGCTCCGT CTTCAGTGGA 
ATCACCCTCA CCAAGTTTGG CGGGATCCTG ATTCTCGCTC TGTCCAAATC GCAGATCTTC 
CAGATTTTCT ACTTCCGCAT GTATCTGGCA ATCGTGCTTC TGGGAGCCGC TCACGGCCTC 
ATCTTCCTGC CTGTCTTACT CAGTTATGCA GGCCCGTCTG TAAATAAAGC AAAGGTTCTA 
GCTGCGCACA ACAGGTTTGT GGGCACAGAG AGAGAGCGGC TCATCTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163716.1
CDS	88..3918
Translation	MLLLGRNHIFRLLLATVLLSHWVHGQHCIWYGECGNSPNVPEKKLNCNYTGPAVPLPNEGQELLQELCPRLVYADNRVCCDTQQLNTLKSNIQIPLQYLSRCPACFFNFMTLFCELTCSPRQSQFISVKDFLKEKNQTSVGNVTYYITQTFADAMFNACRDVQAPSSNIKALGLLCGRDASVCTPQIWIQYMFSISNGQVPFGIEPIFTDVPVQGMTPMNNRTFNCSQSLDDGSEPCSCQDCSEVCGPTPVPPPIPPPWIILGLDAMSFIMWCSYIAFLLIFFGVVLGAWCYRRSVVTSEYGPILDSNQPHSLNSDGTDLIDEASCCETVGERFENSLRLVFSRWGSLCVRQPLTIILSSLVLICICSAGLSYMRITTNPVELWSAPSSRARQEKNYFDQHFGPFFRTEQLIITTPWTEEGGFSTITGDIIPFSPILNLSLLHQVLDLQLEIENLIAEYKGENVTLKDICVSPLSPYNDNCTILSVLNYFQNSHEVLDHEFQDEFFLYNDYHTHLLYCASSPTSLDDTSRLHDPCMGTFGGPVFPWLVLGGYEDSAYNNATALVITFPVTNYLNDTEKLGKALAWEKEFIRFMKNYENPNLTVSFSSERSIEDEIDRESNSDVSTIVISYIIMFVYISVALGRINSCRTLLVDSKISLGIAGILIVLSSVACSLGIFSYIGIPLTLIVIEVIPFLVLAVGVDNIFIIVQTYQRDERMPEEELHQQIGRILGDVAPSMFLSSFSETVAFFLGALSTMPAVRTFSLFAGLAIFIDFLLQISCFVSLLGLDIKRQEANRMDILCCVKLSDGQEEKSEGWLFRFFKKIYAPFILKDWVRPLVVAVFVGMLSFSIAVVNKVEIGLEQTLSMPDDSYVLNYFGNLSKYLHTGPPVYFVVEDGHDYKTFEGQNAVCGGVGCNNDSLVQQIYTASLMSNYTRISNVPSSWLDDYFDWVKPQSTCCRYYNSTGAFCNASVVDKSCVHCRPMTSSGKQRPNGTEFMHFLPMFLSDNPNIKCGKGGHAAYGTAVDLKDNNTDVGATYFMSYHTILKNSSDFINAMKMARELTDNITQTLSTHDKSYKVFPYSVFYVFYEQYLTIVDDTALNLGVSLSAIFIVTAVLLGFELWSAVLVCFTIAMILINMFGVMWLWSISLNAVSLVNLVMSCGISVEFCSHIVRAFSISTRSSRVERAEEALAHMGSSVFSGITLTKFGGILILALSKSQIFQIFYFRMYLAIVLLGAAHGLIFLPVLLSYAGPSVNKAKVLAAHNRFVGTERERLIY

Target ORF information:

RefSeq Version	XM_005163659.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Niemann-Pick disease, type C1 (npc1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163659.4

ORF Insert Sequence:

ATGCTTCTGC TGGGGAGAAA CCACATTTTC CGTCTTTTAT TGGCCACAGT TTTATTATCA 
CATTGGGTGC ATGGCCAGCA CTGTATCTGG TACGGCGAAT GTGGGAACTC CCCAAACGTC 
CCAGAAAAGA AGTTGAACTG CAATTACACC GGTCCTGCTG TGCCTCTGCC TAATGAAGGA 
CAGGAGCTGC TGCAGGAGCT GTGTCCGAGA CTGGTCTACG CAGACAACAG AGTTTGCTGC 
GATACACAAC AGCTCAACAC ACTAAAAAGC AACATCCAGA TCCCCCTTCA GTACCTGTCC 
AGGTGTCCAG CATGTTTCTT CAACTTCATG ACCCTGTTCT GCGAGCTCAC CTGCAGCCCC 
AGACAGAGCC AGTTTATCAG CGTAAAAGAC TTCCTTAAAG AGAAAAACCA GACCAGTGTG 
GGGAATGTGA CCTATTATAT CACCCAGACC TTTGCCGATG CCATGTTTAA CGCCTGCAGA 
GATGTCCAGG CTCCATCCAG CAACATCAAA GCTCTCGGTC TGTTGTGCGG CAGAGACGCC 
AGTGTGTGTA CTCCTCAAAT TTGGATCCAG TACATGTTTA GTATCAGCAA CGGACAGGTT 
CCCTTTGGCA TCGAACCGAT TTTCACAGAT GTCCCTGTGC AGGGCATGAC CCCAATGAAT 
AACAGGACGT TTAACTGCTC TCAGTCTCTG GATGACGGCT CGGAACCCTG CTCGTGTCAG 
GACTGCAGTG AAGTGTGCGG GCCCACGCCT GTGCCCCCTC CCATCCCTCC TCCCTGGATT 
ATCCTCGGCC TGGATGCCAT GAGCTTCATC ATGTGGTGCT CTTACATCGC TTTCCTCCTC 
ATCTTCTTCG GAGTGGTTCT GGGAGCTTGG TGTTACAGAA GGAGCGTGGT CACATCTGAA 
TATGGCCCAA TTCTTGACAG CAATCAGCCC CACTCCTTAA ACTCTGATGG CACAGATTTA 
ATAGATGAGG CGTCGTGCTG TGAGACTGTG GGCGAGCGTT TCGAAAACTC TCTGCGTCTG 
GTGTTCAGCC GCTGGGGCTC TCTGTGCGTC CGGCAGCCGC TCACCATCAT CTTGAGCAGT 
CTGGTGCTGA TCTGCATCTG CTCAGCCGGA CTCTCCTACA TGCGCATCAC CACTAACCCT 
GTGGAGCTCT GGTCTGCTCC CAGCAGCAGA GCACGGCAGG AGAAAAACTA CTTCGACCAG 
CACTTCGGGC CCTTCTTCCG CACCGAGCAG CTCATCATCA CCACTCCATG GACCGAGGAG 
GGAGGTTTCT CCACCATCAC AGGAGACATC ATTCCCTTCT CCCCCATTCT GAACCTATCA 
CTGCTGCATC AGGTGCTGGA TCTGCAGTTG GAAATCGAGA ATCTGATCGC TGAATATAAA 
GGAGAGAATG TGACTCTGAA AGACATCTGT GTTTCACCTT TGTCTCCTTA CAATGACAAC 
TGCACCATCC TGAGTGTGTT AAACTACTTC CAGAACAGCC ATGAAGTCCT GGATCATGAG 
TTTCAAGACG AATTCTTCCT CTACAATGAC TACCACACTC ATCTGCTCTA CTGTGCCAGT 
TCCCCGACCT CTCTGGATGA CACGAGCCGT TTGCATGACC CCTGCATGGG CACGTTCGGA 
GGTCCAGTTT TCCCCTGGCT TGTTCTTGGA GGATATGAGG ACTCCGCCTA CAACAATGCC 
ACCGCTCTGG TGATCACTTT TCCTGTCACC AACTACCTGA ATGACACTGA AAAACTAGGC 
AAAGCTTTGG CATGGGAGAA AGAGTTTATT AGGTTTATGA AGAATTACGA GAACCCGAAC 
CTCACGGTGT CCTTCAGCTC CGAGCGCAGT ATCGAGGACG AGATTGACCG CGAGAGCAAC 
AGTGATGTTT CCACCATCGT GATCAGCTAC ATCATCATGT TTGTCTACAT CTCAGTGGCT 
TTGGGCCGCA TCAACAGCTG CAGGACACTG CTGGTGGACT CTAAGATCTC TCTGGGCATC 
GCTGGGATCC TGATCGTGTT GAGTTCAGTG GCGTGTTCGC TGGGGATCTT CAGCTACATT 
GGGATCCCGC TCACGCTTAT CGTCATTGAG GTCATTCCCT TCCTCGTACT GGCCGTAGGA 
GTGGACAACA TCTTTATCAT TGTGCAGACC TATCAGAGAG ACGAGCGAAT GCCAGAGGAG 
GAGCTTCATC AGCAGATTGG CCGAATCCTC GGAGATGTGG CTCCCAGCAT GTTCCTCTCT 
TCCTTCTCTG AGACCGTGGC CTTCTTTTTA GGTGCTCTGT CCACCATGCC TGCGGTGAGG 
ACGTTCTCTC TGTTCGCTGG TCTGGCCATC TTTATCGACT TCCTCCTGCA GATCAGCTGC 
TTTGTGTCTT TATTGGGCCT CGACATCAAG AGACAAGAGG CCAATCGGAT GGATATATTG 
TGCTGTGTGA AGTTGTCTGA TGGACAAGAA GAAAAATCTG AAGGTTGGCT CTTCCGCTTC 
TTCAAGAAAA TATACGCTCC TTTCATCCTG AAGGACTGGG TTCGCCCTCT GGTGGTTGCT 
GTGTTTGTGG GAATGCTGTC CTTCAGCATT GCCGTTGTTA ATAAAGTGGA GATTGGACTG 
GAGCAGACGC TATCCATGCC TGATGACTCA TATGTGCTGA ATTACTTTGG GAATCTGAGC 
AAGTATCTAC ACACCGGGCC GCCGGTTTAC TTCGTGGTTG AAGATGGCCA TGATTATAAG 
ACGTTTGAGG GCCAGAACGC AGTGTGTGGT GGAGTCGGCT GCAACAACGA CTCTCTGGTC 
CAGCAGATTT ACACCGCTTC CCTCATGAGC AACTACACAA GGATCAGTAA TGTACCCTCG 
TCCTGGCTGG ATGACTACTT TGACTGGGTG AAGCCGCAGT CGACCTGCTG CAGATATTAC 
AACAGCACAG GAGCTTTCTG TAACGCTTCA GTGGTGGACA AGAGCTGCGT TCACTGCAGA 
CCAATGACCT CTAGTGGGAA ACAGAGACCG AACGGCACTG AGTTCATGCA CTTCCTCCCC 
ATGTTTCTCT CTGACAACCC CAATATCAAA TGTGGAAAAG GTGGTCATGC AGCGTACGGC 
ACTGCCGTGG ATCTCAAAGA CAACAACACA GATGTGGGCG CCACATACTT CATGAGTTAC 
CACACCATCC TGAAAAACTC CTCCGACTTT ATTAATGCCA TGAAGATGGC CAGAGAGCTT 
ACAGACAACA TCACACAGAC CCTCAGCACG CATGACAAGA GCTACAAAGT CTTCCCTTAC 
AGTGTGTTTT ACGTCTTCTA CGAGCAGTAT CTGACCATCG TCGATGACAC CGCGCTGAAC 
CTCGGCGTGT CTCTGTCCGC CATCTTCATT GTGACGGCAG TTCTGCTGGG CTTTGAGCTC 
TGGTCGGCGG TGCTCGTCTG CTTCACCATC GCTATGATCC TCATCAACAT GTTCGGGGTC 
ATGTGGCTCT GGAGCATCAG CCTCAACGCT GTCTCATTGG TCAACTTGGT CATGAGTTGT 
GGGATATCAG TGGAGTTCTG CAGTCATATA GTGAGGGCTT TCTCCATCAG CACCAGGAGC 
TCCCGGGTGG AGCGGGCAGA GGAGGCGTTG GCACATATGG GCAGCTCCGT CTTCAGTGGA 
ATCACCCTCA CCAAGTTTGG CGGGATCCTG ATTCTCGCTC TGTCCAAATC GCAGATCTTC 
CAGATTTTCT ACTTCCGCAT GTATCTGGCA ATCGTGCTTC TGGGAGCCGC TCACGGCCTC 
ATCTTCCTGC CTGTCTTACT CAGTTATGCA GGCCCGTCTG TAAATAAAGC AAAGGTTCTA 
GCTGCGCACA ACAGGTTTGT GGGCACAGAG AGAGAGCGGC TCATCTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

npc1 ( XM_005163659.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005163716.1 Danio rerio Niemann-Pick disease, type C1 (npc1), transcript variant X2, mRNA.

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CloneID	ODa06626
Clone ID Related Accession (Same CDS sequence)	XM_005163659.4 , XM_005163659.3
Accession Version	XM_005163659.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3831bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	Niemann-Pick C1 protein isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005163659.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCTGC TGGGGAGAAA CCACATTTTC CGTCTTTTAT TGGCCACAGT TTTATTATCA 
CATTGGGTGC ATGGCCAGCA CTGTATCTGG TACGGCGAAT GTGGGAACTC CCCAAACGTC 
CCAGAAAAGA AGTTGAACTG CAATTACACC GGTCCTGCTG TGCCTCTGCC TAATGAAGGA 
CAGGAGCTGC TGCAGGAGCT GTGTCCGAGA CTGGTCTACG CAGACAACAG AGTTTGCTGC 
GATACACAAC AGCTCAACAC ACTAAAAAGC AACATCCAGA TCCCCCTTCA GTACCTGTCC 
AGGTGTCCAG CATGTTTCTT CAACTTCATG ACCCTGTTCT GCGAGCTCAC CTGCAGCCCC 
AGACAGAGCC AGTTTATCAG CGTAAAAGAC TTCCTTAAAG AGAAAAACCA GACCAGTGTG 
GGGAATGTGA CCTATTATAT CACCCAGACC TTTGCCGATG CCATGTTTAA CGCCTGCAGA 
GATGTCCAGG CTCCATCCAG CAACATCAAA GCTCTCGGTC TGTTGTGCGG CAGAGACGCC 
AGTGTGTGTA CTCCTCAAAT TTGGATCCAG TACATGTTTA GTATCAGCAA CGGACAGGTT 
CCCTTTGGCA TCGAACCGAT TTTCACAGAT GTCCCTGTGC AGGGCATGAC CCCAATGAAT 
AACAGGACGT TTAACTGCTC TCAGTCTCTG GATGACGGCT CGGAACCCTG CTCGTGTCAG 
GACTGCAGTG AAGTGTGCGG GCCCACGCCT GTGCCCCCTC CCATCCCTCC TCCCTGGATT 
ATCCTCGGCC TGGATGCCAT GAGCTTCATC ATGTGGTGCT CTTACATCGC TTTCCTCCTC 
ATCTTCTTCG GAGTGGTTCT GGGAGCTTGG TGTTACAGAA GGAGCGTGGT CACATCTGAA 
TATGGCCCAA TTCTTGACAG CAATCAGCCC CACTCCTTAA ACTCTGATGG CACAGATTTA 
ATAGATGAGG CGTCGTGCTG TGAGACTGTG GGCGAGCGTT TCGAAAACTC TCTGCGTCTG 
GTGTTCAGCC GCTGGGGCTC TCTGTGCGTC CGGCAGCCGC TCACCATCAT CTTGAGCAGT 
CTGGTGCTGA TCTGCATCTG CTCAGCCGGA CTCTCCTACA TGCGCATCAC CACTAACCCT 
GTGGAGCTCT GGTCTGCTCC CAGCAGCAGA GCACGGCAGG AGAAAAACTA CTTCGACCAG 
CACTTCGGGC CCTTCTTCCG CACCGAGCAG CTCATCATCA CCACTCCATG GACCGAGGAG 
GGAGGTTTCT CCACCATCAC AGGAGACATC ATTCCCTTCT CCCCCATTCT GAACCTATCA 
CTGCTGCATC AGGTGCTGGA TCTGCAGTTG GAAATCGAGA ATCTGATCGC TGAATATAAA 
GGAGAGAATG TGACTCTGAA AGACATCTGT GTTTCACCTT TGTCTCCTTA CAATGACAAC 
TGCACCATCC TGAGTGTGTT AAACTACTTC CAGAACAGCC ATGAAGTCCT GGATCATGAG 
TTTCAAGACG AATTCTTCCT CTACAATGAC TACCACACTC ATCTGCTCTA CTGTGCCAGT 
TCCCCGACCT CTCTGGATGA CACGAGCCGT TTGCATGACC CCTGCATGGG CACGTTCGGA 
GGTCCAGTTT TCCCCTGGCT TGTTCTTGGA GGATATGAGG ACTCCGCCTA CAACAATGCC 
ACCGCTCTGG TGATCACTTT TCCTGTCACC AACTACCTGA ATGACACTGA AAAACTAGGC 
AAAGCTTTGG CATGGGAGAA AGAGTTTATT AGGTTTATGA AGAATTACGA GAACCCGAAC 
CTCACGGTGT CCTTCAGCTC CGAGCGCAGT ATCGAGGACG AGATTGACCG CGAGAGCAAC 
AGTGATGTTT CCACCATCGT GATCAGCTAC ATCATCATGT TTGTCTACAT CTCAGTGGCT 
TTGGGCCGCA TCAACAGCTG CAGGACACTG CTGGTGGACT CTAAGATCTC TCTGGGCATC 
GCTGGGATCC TGATCGTGTT GAGTTCAGTG GCGTGTTCGC TGGGGATCTT CAGCTACATT 
GGGATCCCGC TCACGCTTAT CGTCATTGAG GTCATTCCCT TCCTCGTACT GGCCGTAGGA 
GTGGACAACA TCTTTATCAT TGTGCAGACC TATCAGAGAG ACGAGCGAAT GCCAGAGGAG 
GAGCTTCATC AGCAGATTGG CCGAATCCTC GGAGATGTGG CTCCCAGCAT GTTCCTCTCT 
TCCTTCTCTG AGACCGTGGC CTTCTTTTTA GGTGCTCTGT CCACCATGCC TGCGGTGAGG 
ACGTTCTCTC TGTTCGCTGG TCTGGCCATC TTTATCGACT TCCTCCTGCA GATCAGCTGC 
TTTGTGTCTT TATTGGGCCT CGACATCAAG AGACAAGAGG CCAATCGGAT GGATATATTG 
TGCTGTGTGA AGTTGTCTGA TGGACAAGAA GAAAAATCTG AAGGTTGGCT CTTCCGCTTC 
TTCAAGAAAA TATACGCTCC TTTCATCCTG AAGGACTGGG TTCGCCCTCT GGTGGTTGCT 
GTGTTTGTGG GAATGCTGTC CTTCAGCATT GCCGTTGTTA ATAAAGTGGA GATTGGACTG 
GAGCAGACGC TATCCATGCC TGATGACTCA TATGTGCTGA ATTACTTTGG GAATCTGAGC 
AAGTATCTAC ACACCGGGCC GCCGGTTTAC TTCGTGGTTG AAGATGGCCA TGATTATAAG 
ACGTTTGAGG GCCAGAACGC AGTGTGTGGT GGAGTCGGCT GCAACAACGA CTCTCTGGTC 
CAGCAGATTT ACACCGCTTC CCTCATGAGC AACTACACAA GGATCAGTAA TGTACCCTCG 
TCCTGGCTGG ATGACTACTT TGACTGGGTG AAGCCGCAGT CGACCTGCTG CAGATATTAC 
AACAGCACAG GAGCTTTCTG TAACGCTTCA GTGGTGGACA AGAGCTGCGT TCACTGCAGA 
CCAATGACCT CTAGTGGGAA ACAGAGACCG AACGGCACTG AGTTCATGCA CTTCCTCCCC 
ATGTTTCTCT CTGACAACCC CAATATCAAA TGTGGAAAAG GTGGTCATGC AGCGTACGGC 
ACTGCCGTGG ATCTCAAAGA CAACAACACA GATGTGGGCG CCACATACTT CATGAGTTAC 
CACACCATCC TGAAAAACTC CTCCGACTTT ATTAATGCCA TGAAGATGGC CAGAGAGCTT 
ACAGACAACA TCACACAGAC CCTCAGCACG CATGACAAGA GCTACAAAGT CTTCCCTTAC 
AGTGTGTTTT ACGTCTTCTA CGAGCAGTAT CTGACCATCG TCGATGACAC CGCGCTGAAC 
CTCGGCGTGT CTCTGTCCGC CATCTTCATT GTGACGGCAG TTCTGCTGGG CTTTGAGCTC 
TGGTCGGCGG TGCTCGTCTG CTTCACCATC GCTATGATCC TCATCAACAT GTTCGGGGTC 
ATGTGGCTCT GGAGCATCAG CCTCAACGCT GTCTCATTGG TCAACTTGGT CATGAGTTGT 
GGGATATCAG TGGAGTTCTG CAGTCATATA GTGAGGGCTT TCTCCATCAG CACCAGGAGC 
TCCCGGGTGG AGCGGGCAGA GGAGGCGTTG GCACATATGG GCAGCTCCGT CTTCAGTGGA 
ATCACCCTCA CCAAGTTTGG CGGGATCCTG ATTCTCGCTC TGTCCAAATC GCAGATCTTC 
CAGATTTTCT ACTTCCGCAT GTATCTGGCA ATCGTGCTTC TGGGAGCCGC TCACGGCCTC 
ATCTTCCTGC CTGTCTTACT CAGTTATGCA GGCCCGTCTG TAAATAAAGC AAAGGTTCTA 
GCTGCGCACA ACAGGTTTGT GGGCACAGAG AGAGAGCGGC TCATCTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163716.1
CDS	91..3921
Translation	MLLLGRNHIFRLLLATVLLSHWVHGQHCIWYGECGNSPNVPEKKLNCNYTGPAVPLPNEGQELLQELCPRLVYADNRVCCDTQQLNTLKSNIQIPLQYLSRCPACFFNFMTLFCELTCSPRQSQFISVKDFLKEKNQTSVGNVTYYITQTFADAMFNACRDVQAPSSNIKALGLLCGRDASVCTPQIWIQYMFSISNGQVPFGIEPIFTDVPVQGMTPMNNRTFNCSQSLDDGSEPCSCQDCSEVCGPTPVPPPIPPPWIILGLDAMSFIMWCSYIAFLLIFFGVVLGAWCYRRSVVTSEYGPILDSNQPHSLNSDGTDLIDEASCCETVGERFENSLRLVFSRWGSLCVRQPLTIILSSLVLICICSAGLSYMRITTNPVELWSAPSSRARQEKNYFDQHFGPFFRTEQLIITTPWTEEGGFSTITGDIIPFSPILNLSLLHQVLDLQLEIENLIAEYKGENVTLKDICVSPLSPYNDNCTILSVLNYFQNSHEVLDHEFQDEFFLYNDYHTHLLYCASSPTSLDDTSRLHDPCMGTFGGPVFPWLVLGGYEDSAYNNATALVITFPVTNYLNDTEKLGKALAWEKEFIRFMKNYENPNLTVSFSSERSIEDEIDRESNSDVSTIVISYIIMFVYISVALGRINSCRTLLVDSKISLGIAGILIVLSSVACSLGIFSYIGIPLTLIVIEVIPFLVLAVGVDNIFIIVQTYQRDERMPEEELHQQIGRILGDVAPSMFLSSFSETVAFFLGALSTMPAVRTFSLFAGLAIFIDFLLQISCFVSLLGLDIKRQEANRMDILCCVKLSDGQEEKSEGWLFRFFKKIYAPFILKDWVRPLVVAVFVGMLSFSIAVVNKVEIGLEQTLSMPDDSYVLNYFGNLSKYLHTGPPVYFVVEDGHDYKTFEGQNAVCGGVGCNNDSLVQQIYTASLMSNYTRISNVPSSWLDDYFDWVKPQSTCCRYYNSTGAFCNASVVDKSCVHCRPMTSSGKQRPNGTEFMHFLPMFLSDNPNIKCGKGGHAAYGTAVDLKDNNTDVGATYFMSYHTILKNSSDFINAMKMARELTDNITQTLSTHDKSYKVFPYSVFYVFYEQYLTIVDDTALNLGVSLSAIFIVTAVLLGFELWSAVLVCFTIAMILINMFGVMWLWSISLNAVSLVNLVMSCGISVEFCSHIVRAFSISTRSSRVERAEEALAHMGSSVFSGITLTKFGGILILALSKSQIFQIFYFRMYLAIVLLGAAHGLIFLPVLLSYAGPSVNKAKVLAAHNRFVGTERERLIY

Target ORF information:

RefSeq Version	XM_005163659.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Niemann-Pick disease, type C1 (npc1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163659.3

ORF Insert Sequence:

ATGCTTCTGC TGGGGAGAAA CCACATTTTC CGTCTTTTAT TGGCCACAGT TTTATTATCA 
CATTGGGTGC ATGGCCAGCA CTGTATCTGG TACGGCGAAT GTGGGAACTC CCCAAACGTC 
CCAGAAAAGA AGTTGAACTG CAATTACACC GGTCCTGCTG TGCCTCTGCC TAATGAAGGA 
CAGGAGCTGC TGCAGGAGCT GTGTCCGAGA CTGGTCTACG CAGACAACAG AGTTTGCTGC 
GATACACAAC AGCTCAACAC ACTAAAAAGC AACATCCAGA TCCCCCTTCA GTACCTGTCC 
AGGTGTCCAG CATGTTTCTT CAACTTCATG ACCCTGTTCT GCGAGCTCAC CTGCAGCCCC 
AGACAGAGCC AGTTTATCAG CGTAAAAGAC TTCCTTAAAG AGAAAAACCA GACCAGTGTG 
GGGAATGTGA CCTATTATAT CACCCAGACC TTTGCCGATG CCATGTTTAA CGCCTGCAGA 
GATGTCCAGG CTCCATCCAG CAACATCAAA GCTCTCGGTC TGTTGTGCGG CAGAGACGCC 
AGTGTGTGTA CTCCTCAAAT TTGGATCCAG TACATGTTTA GTATCAGCAA CGGACAGGTT 
CCCTTTGGCA TCGAACCGAT TTTCACAGAT GTCCCTGTGC AGGGCATGAC CCCAATGAAT 
AACAGGACGT TTAACTGCTC TCAGTCTCTG GATGACGGCT CGGAACCCTG CTCGTGTCAG 
GACTGCAGTG AAGTGTGCGG GCCCACGCCT GTGCCCCCTC CCATCCCTCC TCCCTGGATT 
ATCCTCGGCC TGGATGCCAT GAGCTTCATC ATGTGGTGCT CTTACATCGC TTTCCTCCTC 
ATCTTCTTCG GAGTGGTTCT GGGAGCTTGG TGTTACAGAA GGAGCGTGGT CACATCTGAA 
TATGGCCCAA TTCTTGACAG CAATCAGCCC CACTCCTTAA ACTCTGATGG CACAGATTTA 
ATAGATGAGG CGTCGTGCTG TGAGACTGTG GGCGAGCGTT TCGAAAACTC TCTGCGTCTG 
GTGTTCAGCC GCTGGGGCTC TCTGTGCGTC CGGCAGCCGC TCACCATCAT CTTGAGCAGT 
CTGGTGCTGA TCTGCATCTG CTCAGCCGGA CTCTCCTACA TGCGCATCAC CACTAACCCT 
GTGGAGCTCT GGTCTGCTCC CAGCAGCAGA GCACGGCAGG AGAAAAACTA CTTCGACCAG 
CACTTCGGGC CCTTCTTCCG CACCGAGCAG CTCATCATCA CCACTCCATG GACCGAGGAG 
GGAGGTTTCT CCACCATCAC AGGAGACATC ATTCCCTTCT CCCCCATTCT GAACCTATCA 
CTGCTGCATC AGGTGCTGGA TCTGCAGTTG GAAATCGAGA ATCTGATCGC TGAATATAAA 
GGAGAGAATG TGACTCTGAA AGACATCTGT GTTTCACCTT TGTCTCCTTA CAATGACAAC 
TGCACCATCC TGAGTGTGTT AAACTACTTC CAGAACAGCC ATGAAGTCCT GGATCATGAG 
TTTCAAGACG AATTCTTCCT CTACAATGAC TACCACACTC ATCTGCTCTA CTGTGCCAGT 
TCCCCGACCT CTCTGGATGA CACGAGCCGT TTGCATGACC CCTGCATGGG CACGTTCGGA 
GGTCCAGTTT TCCCCTGGCT TGTTCTTGGA GGATATGAGG ACTCCGCCTA CAACAATGCC 
ACCGCTCTGG TGATCACTTT TCCTGTCACC AACTACCTGA ATGACACTGA AAAACTAGGC 
AAAGCTTTGG CATGGGAGAA AGAGTTTATT AGGTTTATGA AGAATTACGA GAACCCGAAC 
CTCACGGTGT CCTTCAGCTC CGAGCGCAGT ATCGAGGACG AGATTGACCG CGAGAGCAAC 
AGTGATGTTT CCACCATCGT GATCAGCTAC ATCATCATGT TTGTCTACAT CTCAGTGGCT 
TTGGGCCGCA TCAACAGCTG CAGGACACTG CTGGTGGACT CTAAGATCTC TCTGGGCATC 
GCTGGGATCC TGATCGTGTT GAGTTCAGTG GCGTGTTCGC TGGGGATCTT CAGCTACATT 
GGGATCCCGC TCACGCTTAT CGTCATTGAG GTCATTCCCT TCCTCGTACT GGCCGTAGGA 
GTGGACAACA TCTTTATCAT TGTGCAGACC TATCAGAGAG ACGAGCGAAT GCCAGAGGAG 
GAGCTTCATC AGCAGATTGG CCGAATCCTC GGAGATGTGG CTCCCAGCAT GTTCCTCTCT 
TCCTTCTCTG AGACCGTGGC CTTCTTTTTA GGTGCTCTGT CCACCATGCC TGCGGTGAGG 
ACGTTCTCTC TGTTCGCTGG TCTGGCCATC TTTATCGACT TCCTCCTGCA GATCAGCTGC 
TTTGTGTCTT TATTGGGCCT CGACATCAAG AGACAAGAGG CCAATCGGAT GGATATATTG 
TGCTGTGTGA AGTTGTCTGA TGGACAAGAA GAAAAATCTG AAGGTTGGCT CTTCCGCTTC 
TTCAAGAAAA TATACGCTCC TTTCATCCTG AAGGACTGGG TTCGCCCTCT GGTGGTTGCT 
GTGTTTGTGG GAATGCTGTC CTTCAGCATT GCCGTTGTTA ATAAAGTGGA GATTGGACTG 
GAGCAGACGC TATCCATGCC TGATGACTCA TATGTGCTGA ATTACTTTGG GAATCTGAGC 
AAGTATCTAC ACACCGGGCC GCCGGTTTAC TTCGTGGTTG AAGATGGCCA TGATTATAAG 
ACGTTTGAGG GCCAGAACGC AGTGTGTGGT GGAGTCGGCT GCAACAACGA CTCTCTGGTC 
CAGCAGATTT ACACCGCTTC CCTCATGAGC AACTACACAA GGATCAGTAA TGTACCCTCG 
TCCTGGCTGG ATGACTACTT TGACTGGGTG AAGCCGCAGT CGACCTGCTG CAGATATTAC 
AACAGCACAG GAGCTTTCTG TAACGCTTCA GTGGTGGACA AGAGCTGCGT TCACTGCAGA 
CCAATGACCT CTAGTGGGAA ACAGAGACCG AACGGCACTG AGTTCATGCA CTTCCTCCCC 
ATGTTTCTCT CTGACAACCC CAATATCAAA TGTGGAAAAG GTGGTCATGC AGCGTACGGC 
ACTGCCGTGG ATCTCAAAGA CAACAACACA GATGTGGGCG CCACATACTT CATGAGTTAC 
CACACCATCC TGAAAAACTC CTCCGACTTT ATTAATGCCA TGAAGATGGC CAGAGAGCTT 
ACAGACAACA TCACACAGAC CCTCAGCACG CATGACAAGA GCTACAAAGT CTTCCCTTAC 
AGTGTGTTTT ACGTCTTCTA CGAGCAGTAT CTGACCATCG TCGATGACAC CGCGCTGAAC 
CTCGGCGTGT CTCTGTCCGC CATCTTCATT GTGACGGCAG TTCTGCTGGG CTTTGAGCTC 
TGGTCGGCGG TGCTCGTCTG CTTCACCATC GCTATGATCC TCATCAACAT GTTCGGGGTC 
ATGTGGCTCT GGAGCATCAG CCTCAACGCT GTCTCATTGG TCAACTTGGT CATGAGTTGT 
GGGATATCAG TGGAGTTCTG CAGTCATATA GTGAGGGCTT TCTCCATCAG CACCAGGAGC 
TCCCGGGTGG AGCGGGCAGA GGAGGCGTTG GCACATATGG GCAGCTCCGT CTTCAGTGGA 
ATCACCCTCA CCAAGTTTGG CGGGATCCTG ATTCTCGCTC TGTCCAAATC GCAGATCTTC 
CAGATTTTCT ACTTCCGCAT GTATCTGGCA ATCGTGCTTC TGGGAGCCGC TCACGGCCTC 
ATCTTCCTGC CTGTCTTACT CAGTTATGCA GGCCCGTCTG TAAATAAAGC AAAGGTTCTA 
GCTGCGCACA ACAGGTTTGT GGGCACAGAG AGAGAGCGGC TCATCTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pla2g6 Deficiency in Zebrafish Leads to Dopaminergic Cell Death, Axonal Degeneration, Increased ?-Synuclein Expression, and Defects in Brain Functions and Pathways.
Molecular neurobiology(2018 Jan)
S?nchez E,Azcona LJ,Pais?n-Ruiz C

Dietary lipid content reorganizes gut microbiota and probiotic L. rhamnosus attenuates obesity and enhances catabolic hormonal milieu in zebrafish.
Scientific reports7(1)5512(2017 Jul)
Falcinelli S,Rodiles A,Hatef A,Picchietti S,Cossignani L,Merrifield DL,Unniappan S,Carnevali O

Altered Adipogenesis in Zebrafish Larvae Following High Fat Diet and Chemical Exposure Is Visualised by Stimulated Raman Scattering Microscopy.
International journal of molecular sciences18(4)(2017 Apr)
den Broeder MJ,Moester MJB,Kamstra JH,Cenijn PH,Davidoiu V,Kamminga LM,Ariese F,de Boer JF,Legler J

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npc1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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