zgc:113333 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	zgc:113333
Entrez Gene ID	541364
Full Name	zgc:113333
Synonyms	si:ch211-173k16.6
General protein information	Preferred Names zgc:113333 Names hexosaminidase D
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005163875.3	XP_005163932.1	hexosaminidase D isoform X1
XM_009299336.2	XP_009297611.1	hexosaminidase D isoform X1
NM_001013491.1	NP_001013509.1	hexosaminidase D
XM_005163876.3	XP_005163933.1	hexosaminidase D isoform X1
XM_005163875.4	XP_005163932.1	hexosaminidase D isoform X1
XM_005163876.4	XP_005163933.1	hexosaminidase D isoform X1
XM_009299336.3	XP_009297611.1	hexosaminidase D isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	HEXDC	XP_001234313.3
Xenopus tropicalis (tropical clawed frog)	LOC100494095	XP_004918100.1
Caenorhabditis elegans (roundworm)	hex-4	NP_740792.3
Danio rerio (zebrafish)	zgc:113333	NP_001013509.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

A live zebrafish-based screening system for human nuclear receptor ligand and cofactor discovery.
Tiefenbach J, Moll PR, Nelson MR, Hu C, Baev L, Kislinger T, Krause HM
PloS one5(3)e9797(2010 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following zgc:113333 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the zgc:113333 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa34434	NM_001013491.1 Latest version!	Danio rerio zgc:113333 (zgc:113333), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa08151	XM_009299336.3 Latest version!	Danio rerio zgc:113333 (zgc:113333), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa08151	XM_005163876.4 Latest version!	Danio rerio zgc:113333 (zgc:113333), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa08151	XM_005163875.4 Latest version!	Danio rerio zgc:113333 (zgc:113333), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
View Old Accession Versions >>
ODa08151	XM_005163875.3	Danio rerio zgc:113333 (zgc:113333), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa08151	XM_005163876.3	Danio rerio zgc:113333 (zgc:113333), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa08151	XM_009299336.2	Danio rerio zgc:113333 (zgc:113333), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

zgc:113333 ( NM_001013491.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001013509.1 Danio rerio zgc:113333 (zgc:113333), mRNA.

Price & Availability↑

CloneID	ODa34434
Clone ID Related Accession (Same CDS sequence)	NM_001013491.1
Accession Version	NM_001013491.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	hexosaminidase D
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC090449.1. On Jul 6, 2005 this sequence version replaced XM_695646.1. ##Evidence-Data-START## Transcript exon combination :: BC090449.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGAGCAGCC AAAAGCTTAT TAGGTTAATC ATCGGCTCCT TAGTGATTGT GGCCATGGTG 
AAACTGTTCT TCAACCCCAG AGGAAGTGTG AAGTCTAAAA GTGAAAAGAC CCACCAGATC 
ATACAAGGTG GTAATTTCTG GGCTAGCCAA CGAATCCCAC TGATAAAAGT GGTTGAAAAT 
AAACAGGATA CCGTGAAAAC AGAACAACAG CCTAAAGTTG AGCAAGAGGA AAGTGAGAAG 
ATTCAGAAAA AACAAGAGCA ATTAAAGCCA GAAGTTCCTG AAAAAACAGA TCCACTAAAG 
ATTGTGCATT TAGATCTGAA AGGTGCCCCA CCCAAAGTTA AATACCTTGA GCAGTTATTC 
CCCCTACTGT CATCTTTGGG TGCTAATGGG ATATTGCTGG AATATGAGGA CATGTTTCCC 
TATGAGGGAG ACCTTGAAAT TCTCAAATCA AGTTTTGCAT ACAGTCCGGA AGATGTAGAG 
GAAATTAAGT CTCTGGCTAA ACGACAAAAT CTGGAACTGA TACCTTTGGT GCAGGTGTTT 
GGACACATGG AGTTCGTCTT AAAACATGAA AAGTATTTTA AATTGAGAGA AGTGGAAACG 
TTTCCCAATA GTCTAAACTC TCTGGCCCAA GGAAGCATGG AACTTGTTCA AAACATGCTA 
ACGCAGGTTA TGAAGAAGCA CCCAGAAGCA CGGTGGTTCC ACATTGGTGC CGATGAGGTG 
CGTGGCTTGG GCGAAAGTGA AGATTCGAAG CGATGGTTAG AAGCCAACAA CGGAGACATG 
GGCAAGCTCT ACCTCAATCA TGTGACTGCA GTTGCAAAGT TTATGACCAA ACAGAAGCAA 
AGAATCAAGC TGATCTTGTG GGACGACATG TTCAGGAAAT TTAGCCCTGA CACTATAAAA 
GAATCAGGTT TGCAAGATCT TGCTTCACCC ATGATATGGA ACTACCATGC AAACTTGAAC 
GTGAAGGACA TTGGTAAGTA TATCTCCAAG TATGAGCAGG CTGGATTTCG TGGCGTGTGG 
TTTGCTAGTG CTTTCAAAGG AGCATCTGGG ATCGACCAGA TCTGGACTCC AATTGATCAC 
CATTTAAAAA ACCATATACA GTGGCAGGAA GTCATCGCAT CCATGCCGCA GTACAAGTCT 
GTGAGATTTC AAGGCATTGC ACTGACTGGC TGGCAGAGGT ATGAGCATCA CACAGTGCTG 
TGCGAGCTTC TCCCGGTGGC CATTCCTTCT CTCACTGTCT GTCTGCAGAC ACTGAAATAT 
GGTGCATTTA ATGCTAAGGC CCAGGGTGAG GTCCAGCGCA TGCTTGGCTG TGAAATTCAG 
CTCGAAAAAA ATGACTGGTA TGAAACAGTT TATATTTTCA ATGACTTTAT TGTTGTCAGC 
ATCAATGGAA GTATCTACTG TAAATGTAAT ATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001013509.1
CDS	249..1664
Translation	MSSQKLIRLIIGSLVIVAMVKLFFNPRGSVKSKSEKTHQIIQGGNFWASQRIPLIKVVENKQDTVKTEQQPKVEQEESEKIQKKQEQLKPEVPEKTDPLKIVHLDLKGAPPKVKYLEQLFPLLSSLGANGILLEYEDMFPYEGDLEILKSSFAYSPEDVEEIKSLAKRQNLELIPLVQVFGHMEFVLKHEKYFKLREVETFPNSLNSLAQGSMELVQNMLTQVMKKHPEARWFHIGADEVRGLGESEDSKRWLEANNGDMGKLYLNHVTAVAKFMTKQKQRIKLILWDDMFRKFSPDTIKESGLQDLASPMIWNYHANLNVKDIGKYISKYEQAGFRGVWFASAFKGASGIDQIWTPIDHHLKNHIQWQEVIASMPQYKSVRFQGIALTGWQRYEHHTVLCELLPVAIPSLTVCLQTLKYGAFNAKAQGEVQRMLGCEIQLEKNDWYETVYIFNDFIVVSINGSIYCKCNM

Target ORF information:

RefSeq Version	NM_001013491.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio zgc:113333 (zgc:113333), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001013491.1

ORF Insert Sequence:

ATGAGCAGCC AAAAGCTTAT TAGGTTAATC ATCGGCTCCT TAGTGATTGT GGCCATGGTG 
AAACTGTTCT TCAACCCCAG AGGAAGTGTG AAGTCTAAAA GTGAAAAGAC CCACCAGATC 
ATACAAGGTG GTAATTTCTG GGCTAGCCAA CGAATCCCAC TGATAAAAGT GGTTGAAAAT 
AAACAGGATA CCGTGAAAAC AGAACAACAG CCTAAAGTTG AGCAAGAGGA AAGTGAGAAG 
ATTCAGAAAA AACAAGAGCA ATTAAAGCCA GAAGTTCCTG AAAAAACAGA TCCACTAAAG 
ATTGTGCATT TAGATCTGAA AGGTGCCCCA CCCAAAGTTA AATACCTTGA GCAGTTATTC 
CCCCTACTGT CATCTTTGGG TGCTAATGGG ATATTGCTGG AATATGAGGA CATGTTTCCC 
TATGAGGGAG ACCTTGAAAT TCTCAAATCA AGTTTTGCAT ACAGTCCGGA AGATGTAGAG 
GAAATTAAGT CTCTGGCTAA ACGACAAAAT CTGGAACTGA TACCTTTGGT GCAGGTGTTT 
GGACACATGG AGTTCGTCTT AAAACATGAA AAGTATTTTA AATTGAGAGA AGTGGAAACG 
TTTCCCAATA GTCTAAACTC TCTGGCCCAA GGAAGCATGG AACTTGTTCA AAACATGCTA 
ACGCAGGTTA TGAAGAAGCA CCCAGAAGCA CGGTGGTTCC ACATTGGTGC CGATGAGGTG 
CGTGGCTTGG GCGAAAGTGA AGATTCGAAG CGATGGTTAG AAGCCAACAA CGGAGACATG 
GGCAAGCTCT ACCTCAATCA TGTGACTGCA GTTGCAAAGT TTATGACCAA ACAGAAGCAA 
AGAATCAAGC TGATCTTGTG GGACGACATG TTCAGGAAAT TTAGCCCTGA CACTATAAAA 
GAATCAGGTT TGCAAGATCT TGCTTCACCC ATGATATGGA ACTACCATGC AAACTTGAAC 
GTGAAGGACA TTGGTAAGTA TATCTCCAAG TATGAGCAGG CTGGATTTCG TGGCGTGTGG 
TTTGCTAGTG CTTTCAAAGG AGCATCTGGG ATCGACCAGA TCTGGACTCC AATTGATCAC 
CATTTAAAAA ACCATATACA GTGGCAGGAA GTCATCGCAT CCATGCCGCA GTACAAGTCT 
GTGAGATTTC AAGGCATTGC ACTGACTGGC TGGCAGAGGT ATGAGCATCA CACAGTGCTG 
TGCGAGCTTC TCCCGGTGGC CATTCCTTCT CTCACTGTCT GTCTGCAGAC ACTGAAATAT 
GGTGCATTTA ATGCTAAGGC CCAGGGTGAG GTCCAGCGCA TGCTTGGCTG TGAAATTCAG 
CTCGAAAAAA ATGACTGGTA TGAAACAGTT TATATTTTCA ATGACTTTAT TGTTGTCAGC 
ATCAATGGAA GTATCTACTG TAAATGTAAT ATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

zgc:113333 ( XM_005163875.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005163932.1 Danio rerio zgc:113333 (zgc:113333), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa08151
Clone ID Related Accession (Same CDS sequence)	XM_005163875.3 , XM_009299336.2 , XM_005163876.3 , XM_005163875.4 , XM_005163876.4 , XM_009299336.3
Accession Version	XM_005163875.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	hexosaminidase D isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005163875.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGCC AAAAGCTTAT TAGGTTAATC ATCGGCTCCT TAGTGATTGT GGCCATGGTG 
AAACTGTTCT TCAACCCCAG AGGAAGTGTG AAGTCTAAAA GTGAAAAGAC CCACCAGATC 
ATACAAGGTG GTAATTTCTG GGCTAGCCAA CGAATCCCAC TGATAAAAGT GGTTGAAAAT 
AAACAGGATA CCGTGAAAAC AGAACAACAG CCTAAAGTTG AGCAAGAGGA AAGTGAGAAG 
ATTCAGAAAA AACAAGAGCA ATTAAAGCCA GAAGTTCCTG AAAAAACAGA TCCACTAAAG 
ATTGTGCATT TAGATCTGAA AGGTGCCCCA CCCAAAGTTA AATACCTTGA GCAGTTATTC 
CCTCTACTGT CATCTTTGGG TGCTAATGGG ATATTGCTGG AATATGAGGA CATGTTTCCC 
TATGAGGGAG ACCTTGAAAT TCTCAAATCA AGCTTTGCAT ACAGTCCGGA AGATATAGAG 
GAAATTAAGT CTCTGGCTAA ACGACACAAT CTGGAACTGA TACCTTTGGT GCAGGTGTTT 
GGACACATGG AGTTCGTCTT AAAACATGAA AAGTATTTTA AATTGAGAGA AGTGGAAACG 
TTTCCCAATA GTCTAAACTC TCTGGCCCAA GGAAGCATGG AACTCGTTCA AAACATGCTA 
ATGCAGGTTA TGAAGAAGCA CCCAGAAGCA CGGTGGTTCC ACATTGGTGC CGATGAGGTG 
CGTGGCTTGG GCGAAAGTGA AGATTCGAAG CGATGGTTAG AAGCCAACAA CGGAGACATG 
GGCAAGCTCT ACCTCAATCA TGTGACTGCA GTTGCAAAGT TTATGACCAA ACAGAAGCAA 
AGAATCAAGC TGATCTTGTG GGACGACATG TTCAGGAAAT TTAGCCCTGA CACTATAAAA 
GAATCAGGTT TGCAAGATCT TGCTTCACCC ATGATATGGA ACTACCATGC AAACTTGAAC 
GTGAAGGACA TTGGTAAGTA TATCTCCAAG TATGAGCAGG CTGGATTTCG TGGCGTGTGG 
TTTGCTAGTG CTTTCAAAGG AGCATCTGGG ATCGACCAGA TCTGGACTCC AATTGATCAC 
CATTTAAAAA ACCATATACA GTGGCAGGAA GTCATCGCAT CCATGCCGCA GTACAAGTCA 
GTGAGATTTC AAGGCATTGC ACTGACTGGC TGGCAAAGGT ATGAGCATCA CACAGTGCTG 
TGCGAGCTTC TCCCGGTGGC CATTCCTTCT CTCACTGTCT GTCTGCAGAC ACTGAAATAT 
GGTGCATTTA ATGCTAAGGC CCAGGGTGAG GTCCAGCGCA TGCTTGGCTG TGAAATTCAG 
CTCGAAAAAA ATGACTGTAA GGGTGCATCC TTTCCTGGAT CAGACATTTA TGAAATGGTG 
AAGAAGATCC ACACCAACCT TCAGAGTTCA ATTATAAACC TCAAGCAAAA CTATCACGTC 
AAAGGATCAT TCAGCTTTTA TCATCGCAAA CGCAACTTTG CCAACCCTCG AAATCTTGGA 
TTTTTCAAAG ACGATCTGAA AAAAATGATG GATGAATGGG ATTCCTTTTT GCTGACTTTT 
CGGGAAAGCA TGGAGGCGAT TTTTTACCCG GACACTTTAG AGGAGTGGTT GGAAGAGAAC 
GTGAATGAAC ACATTGAAAC GCTTCACAAC ATGGTGGTTG ATGTAGAGCG GATCATTAAA 
CTCAACGGAC AGCCAAAGGT GGACCCCTTG GAAAATAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163932.1
CDS	517..2238
Translation	MSSQKLIRLIIGSLVIVAMVKLFFNPRGSVKSKSEKTHQIIQGGNFWASQRIPLIKVVENKQDTVKTEQQPKVEQEESEKIQKKQEQLKPEVPEKTDPLKIVHLDLKGAPPKVKYLEQLFPLLSSLGANGILLEYEDMFPYEGDLEILKSSFAYSPEDIEEIKSLAKRHNLELIPLVQVFGHMEFVLKHEKYFKLREVETFPNSLNSLAQGSMELVQNMLMQVMKKHPEARWFHIGADEVRGLGESEDSKRWLEANNGDMGKLYLNHVTAVAKFMTKQKQRIKLILWDDMFRKFSPDTIKESGLQDLASPMIWNYHANLNVKDIGKYISKYEQAGFRGVWFASAFKGASGIDQIWTPIDHHLKNHIQWQEVIASMPQYKSVRFQGIALTGWQRYEHHTVLCELLPVAIPSLTVCLQTLKYGAFNAKAQGEVQRMLGCEIQLEKNDCKGASFPGSDIYEMVKKIHTNLQSSIINLKQNYHVKGSFSFYHRKRNFANPRNLGFFKDDLKKMMDEWDSFLLTFRESMEAIFYPDTLEEWLEENVNEHIETLHNMVVDVERIIKLNGQPKVDPLENR

Target ORF information:

RefSeq Version	XM_005163875.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio zgc:113333 (zgc:113333), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163875.3

ORF Insert Sequence:

ATGAGCAGCC AAAAGCTTAT TAGGTTAATC ATCGGCTCCT TAGTGATTGT GGCCATGGTG 
AAACTGTTCT TCAACCCCAG AGGAAGTGTG AAGTCTAAAA GTGAAAAGAC CCACCAGATC 
ATACAAGGTG GTAATTTCTG GGCTAGCCAA CGAATCCCAC TGATAAAAGT GGTTGAAAAT 
AAACAGGATA CCGTGAAAAC AGAACAACAG CCTAAAGTTG AGCAAGAGGA AAGTGAGAAG 
ATTCAGAAAA AACAAGAGCA ATTAAAGCCA GAAGTTCCTG AAAAAACAGA TCCACTAAAG 
ATTGTGCATT TAGATCTGAA AGGTGCCCCA CCCAAAGTTA AATACCTTGA GCAGTTATTC 
CCTCTACTGT CATCTTTGGG TGCTAATGGG ATATTGCTGG AATATGAGGA CATGTTTCCC 
TATGAGGGAG ACCTTGAAAT TCTCAAATCA AGCTTTGCAT ACAGTCCGGA AGATATAGAG 
GAAATTAAGT CTCTGGCTAA ACGACACAAT CTGGAACTGA TACCTTTGGT GCAGGTGTTT 
GGACACATGG AGTTCGTCTT AAAACATGAA AAGTATTTTA AATTGAGAGA AGTGGAAACG 
TTTCCCAATA GTCTAAACTC TCTGGCCCAA GGAAGCATGG AACTCGTTCA AAACATGCTA 
ATGCAGGTTA TGAAGAAGCA CCCAGAAGCA CGGTGGTTCC ACATTGGTGC CGATGAGGTG 
CGTGGCTTGG GCGAAAGTGA AGATTCGAAG CGATGGTTAG AAGCCAACAA CGGAGACATG 
GGCAAGCTCT ACCTCAATCA TGTGACTGCA GTTGCAAAGT TTATGACCAA ACAGAAGCAA 
AGAATCAAGC TGATCTTGTG GGACGACATG TTCAGGAAAT TTAGCCCTGA CACTATAAAA 
GAATCAGGTT TGCAAGATCT TGCTTCACCC ATGATATGGA ACTACCATGC AAACTTGAAC 
GTGAAGGACA TTGGTAAGTA TATCTCCAAG TATGAGCAGG CTGGATTTCG TGGCGTGTGG 
TTTGCTAGTG CTTTCAAAGG AGCATCTGGG ATCGACCAGA TCTGGACTCC AATTGATCAC 
CATTTAAAAA ACCATATACA GTGGCAGGAA GTCATCGCAT CCATGCCGCA GTACAAGTCA 
GTGAGATTTC AAGGCATTGC ACTGACTGGC TGGCAAAGGT ATGAGCATCA CACAGTGCTG 
TGCGAGCTTC TCCCGGTGGC CATTCCTTCT CTCACTGTCT GTCTGCAGAC ACTGAAATAT 
GGTGCATTTA ATGCTAAGGC CCAGGGTGAG GTCCAGCGCA TGCTTGGCTG TGAAATTCAG 
CTCGAAAAAA ATGACTGTAA GGGTGCATCC TTTCCTGGAT CAGACATTTA TGAAATGGTG 
AAGAAGATCC ACACCAACCT TCAGAGTTCA ATTATAAACC TCAAGCAAAA CTATCACGTC 
AAAGGATCAT TCAGCTTTTA TCATCGCAAA CGCAACTTTG CCAACCCTCG AAATCTTGGA 
TTTTTCAAAG ACGATCTGAA AAAAATGATG GATGAATGGG ATTCCTTTTT GCTGACTTTT 
CGGGAAAGCA TGGAGGCGAT TTTTTACCCG GACACTTTAG AGGAGTGGTT GGAAGAGAAC 
GTGAATGAAC ACATTGAAAC GCTTCACAAC ATGGTGGTTG ATGTAGAGCG GATCATTAAA 
CTCAACGGAC AGCCAAAGGT GGACCCCTTG GAAAATAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

zgc:113333 ( XM_009299336.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009297611.1 Danio rerio zgc:113333 (zgc:113333), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa08151
Clone ID Related Accession (Same CDS sequence)	XM_005163875.3 , XM_009299336.2 , XM_005163876.3 , XM_005163875.4 , XM_005163876.4 , XM_009299336.3
Accession Version	XM_009299336.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	hexosaminidase D isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299336.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGCC AAAAGCTTAT TAGGTTAATC ATCGGCTCCT TAGTGATTGT GGCCATGGTG 
AAACTGTTCT TCAACCCCAG AGGAAGTGTG AAGTCTAAAA GTGAAAAGAC CCACCAGATC 
ATACAAGGTG GTAATTTCTG GGCTAGCCAA CGAATCCCAC TGATAAAAGT GGTTGAAAAT 
AAACAGGATA CCGTGAAAAC AGAACAACAG CCTAAAGTTG AGCAAGAGGA AAGTGAGAAG 
ATTCAGAAAA AACAAGAGCA ATTAAAGCCA GAAGTTCCTG AAAAAACAGA TCCACTAAAG 
ATTGTGCATT TAGATCTGAA AGGTGCCCCA CCCAAAGTTA AATACCTTGA GCAGTTATTC 
CCTCTACTGT CATCTTTGGG TGCTAATGGG ATATTGCTGG AATATGAGGA CATGTTTCCC 
TATGAGGGAG ACCTTGAAAT TCTCAAATCA AGCTTTGCAT ACAGTCCGGA AGATATAGAG 
GAAATTAAGT CTCTGGCTAA ACGACACAAT CTGGAACTGA TACCTTTGGT GCAGGTGTTT 
GGACACATGG AGTTCGTCTT AAAACATGAA AAGTATTTTA AATTGAGAGA AGTGGAAACG 
TTTCCCAATA GTCTAAACTC TCTGGCCCAA GGAAGCATGG AACTCGTTCA AAACATGCTA 
ATGCAGGTTA TGAAGAAGCA CCCAGAAGCA CGGTGGTTCC ACATTGGTGC CGATGAGGTG 
CGTGGCTTGG GCGAAAGTGA AGATTCGAAG CGATGGTTAG AAGCCAACAA CGGAGACATG 
GGCAAGCTCT ACCTCAATCA TGTGACTGCA GTTGCAAAGT TTATGACCAA ACAGAAGCAA 
AGAATCAAGC TGATCTTGTG GGACGACATG TTCAGGAAAT TTAGCCCTGA CACTATAAAA 
GAATCAGGTT TGCAAGATCT TGCTTCACCC ATGATATGGA ACTACCATGC AAACTTGAAC 
GTGAAGGACA TTGGTAAGTA TATCTCCAAG TATGAGCAGG CTGGATTTCG TGGCGTGTGG 
TTTGCTAGTG CTTTCAAAGG AGCATCTGGG ATCGACCAGA TCTGGACTCC AATTGATCAC 
CATTTAAAAA ACCATATACA GTGGCAGGAA GTCATCGCAT CCATGCCGCA GTACAAGTCA 
GTGAGATTTC AAGGCATTGC ACTGACTGGC TGGCAAAGGT ATGAGCATCA CACAGTGCTG 
TGCGAGCTTC TCCCGGTGGC CATTCCTTCT CTCACTGTCT GTCTGCAGAC ACTGAAATAT 
GGTGCATTTA ATGCTAAGGC CCAGGGTGAG GTCCAGCGCA TGCTTGGCTG TGAAATTCAG 
CTCGAAAAAA ATGACTGTAA GGGTGCATCC TTTCCTGGAT CAGACATTTA TGAAATGGTG 
AAGAAGATCC ACACCAACCT TCAGAGTTCA ATTATAAACC TCAAGCAAAA CTATCACGTC 
AAAGGATCAT TCAGCTTTTA TCATCGCAAA CGCAACTTTG CCAACCCTCG AAATCTTGGA 
TTTTTCAAAG ACGATCTGAA AAAAATGATG GATGAATGGG ATTCCTTTTT GCTGACTTTT 
CGGGAAAGCA TGGAGGCGAT TTTTTACCCG GACACTTTAG AGGAGTGGTT GGAAGAGAAC 
GTGAATGAAC ACATTGAAAC GCTTCACAAC ATGGTGGTTG ATGTAGAGCG GATCATTAAA 
CTCAACGGAC AGCCAAAGGT GGACCCCTTG GAAAATAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297611.1
CDS	383..2104
Translation	MSSQKLIRLIIGSLVIVAMVKLFFNPRGSVKSKSEKTHQIIQGGNFWASQRIPLIKVVENKQDTVKTEQQPKVEQEESEKIQKKQEQLKPEVPEKTDPLKIVHLDLKGAPPKVKYLEQLFPLLSSLGANGILLEYEDMFPYEGDLEILKSSFAYSPEDIEEIKSLAKRHNLELIPLVQVFGHMEFVLKHEKYFKLREVETFPNSLNSLAQGSMELVQNMLMQVMKKHPEARWFHIGADEVRGLGESEDSKRWLEANNGDMGKLYLNHVTAVAKFMTKQKQRIKLILWDDMFRKFSPDTIKESGLQDLASPMIWNYHANLNVKDIGKYISKYEQAGFRGVWFASAFKGASGIDQIWTPIDHHLKNHIQWQEVIASMPQYKSVRFQGIALTGWQRYEHHTVLCELLPVAIPSLTVCLQTLKYGAFNAKAQGEVQRMLGCEIQLEKNDCKGASFPGSDIYEMVKKIHTNLQSSIINLKQNYHVKGSFSFYHRKRNFANPRNLGFFKDDLKKMMDEWDSFLLTFRESMEAIFYPDTLEEWLEENVNEHIETLHNMVVDVERIIKLNGQPKVDPLENR

Target ORF information:

RefSeq Version	XM_009299336.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio zgc:113333 (zgc:113333), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299336.2

ORF Insert Sequence:

ATGAGCAGCC AAAAGCTTAT TAGGTTAATC ATCGGCTCCT TAGTGATTGT GGCCATGGTG 
AAACTGTTCT TCAACCCCAG AGGAAGTGTG AAGTCTAAAA GTGAAAAGAC CCACCAGATC 
ATACAAGGTG GTAATTTCTG GGCTAGCCAA CGAATCCCAC TGATAAAAGT GGTTGAAAAT 
AAACAGGATA CCGTGAAAAC AGAACAACAG CCTAAAGTTG AGCAAGAGGA AAGTGAGAAG 
ATTCAGAAAA AACAAGAGCA ATTAAAGCCA GAAGTTCCTG AAAAAACAGA TCCACTAAAG 
ATTGTGCATT TAGATCTGAA AGGTGCCCCA CCCAAAGTTA AATACCTTGA GCAGTTATTC 
CCTCTACTGT CATCTTTGGG TGCTAATGGG ATATTGCTGG AATATGAGGA CATGTTTCCC 
TATGAGGGAG ACCTTGAAAT TCTCAAATCA AGCTTTGCAT ACAGTCCGGA AGATATAGAG 
GAAATTAAGT CTCTGGCTAA ACGACACAAT CTGGAACTGA TACCTTTGGT GCAGGTGTTT 
GGACACATGG AGTTCGTCTT AAAACATGAA AAGTATTTTA AATTGAGAGA AGTGGAAACG 
TTTCCCAATA GTCTAAACTC TCTGGCCCAA GGAAGCATGG AACTCGTTCA AAACATGCTA 
ATGCAGGTTA TGAAGAAGCA CCCAGAAGCA CGGTGGTTCC ACATTGGTGC CGATGAGGTG 
CGTGGCTTGG GCGAAAGTGA AGATTCGAAG CGATGGTTAG AAGCCAACAA CGGAGACATG 
GGCAAGCTCT ACCTCAATCA TGTGACTGCA GTTGCAAAGT TTATGACCAA ACAGAAGCAA 
AGAATCAAGC TGATCTTGTG GGACGACATG TTCAGGAAAT TTAGCCCTGA CACTATAAAA 
GAATCAGGTT TGCAAGATCT TGCTTCACCC ATGATATGGA ACTACCATGC AAACTTGAAC 
GTGAAGGACA TTGGTAAGTA TATCTCCAAG TATGAGCAGG CTGGATTTCG TGGCGTGTGG 
TTTGCTAGTG CTTTCAAAGG AGCATCTGGG ATCGACCAGA TCTGGACTCC AATTGATCAC 
CATTTAAAAA ACCATATACA GTGGCAGGAA GTCATCGCAT CCATGCCGCA GTACAAGTCA 
GTGAGATTTC AAGGCATTGC ACTGACTGGC TGGCAAAGGT ATGAGCATCA CACAGTGCTG 
TGCGAGCTTC TCCCGGTGGC CATTCCTTCT CTCACTGTCT GTCTGCAGAC ACTGAAATAT 
GGTGCATTTA ATGCTAAGGC CCAGGGTGAG GTCCAGCGCA TGCTTGGCTG TGAAATTCAG 
CTCGAAAAAA ATGACTGTAA GGGTGCATCC TTTCCTGGAT CAGACATTTA TGAAATGGTG 
AAGAAGATCC ACACCAACCT TCAGAGTTCA ATTATAAACC TCAAGCAAAA CTATCACGTC 
AAAGGATCAT TCAGCTTTTA TCATCGCAAA CGCAACTTTG CCAACCCTCG AAATCTTGGA 
TTTTTCAAAG ACGATCTGAA AAAAATGATG GATGAATGGG ATTCCTTTTT GCTGACTTTT 
CGGGAAAGCA TGGAGGCGAT TTTTTACCCG GACACTTTAG AGGAGTGGTT GGAAGAGAAC 
GTGAATGAAC ACATTGAAAC GCTTCACAAC ATGGTGGTTG ATGTAGAGCG GATCATTAAA 
CTCAACGGAC AGCCAAAGGT GGACCCCTTG GAAAATAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

zgc:113333 ( XM_005163876.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005163933.1 Danio rerio zgc:113333 (zgc:113333), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa08151
Clone ID Related Accession (Same CDS sequence)	XM_005163875.3 , XM_009299336.2 , XM_005163876.3 , XM_005163875.4 , XM_005163876.4 , XM_009299336.3
Accession Version	XM_005163876.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	hexosaminidase D isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005163876.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGCC AAAAGCTTAT TAGGTTAATC ATCGGCTCCT TAGTGATTGT GGCCATGGTG 
AAACTGTTCT TCAACCCCAG AGGAAGTGTG AAGTCTAAAA GTGAAAAGAC CCACCAGATC 
ATACAAGGTG GTAATTTCTG GGCTAGCCAA CGAATCCCAC TGATAAAAGT GGTTGAAAAT 
AAACAGGATA CCGTGAAAAC AGAACAACAG CCTAAAGTTG AGCAAGAGGA AAGTGAGAAG 
ATTCAGAAAA AACAAGAGCA ATTAAAGCCA GAAGTTCCTG AAAAAACAGA TCCACTAAAG 
ATTGTGCATT TAGATCTGAA AGGTGCCCCA CCCAAAGTTA AATACCTTGA GCAGTTATTC 
CCTCTACTGT CATCTTTGGG TGCTAATGGG ATATTGCTGG AATATGAGGA CATGTTTCCC 
TATGAGGGAG ACCTTGAAAT TCTCAAATCA AGCTTTGCAT ACAGTCCGGA AGATATAGAG 
GAAATTAAGT CTCTGGCTAA ACGACACAAT CTGGAACTGA TACCTTTGGT GCAGGTGTTT 
GGACACATGG AGTTCGTCTT AAAACATGAA AAGTATTTTA AATTGAGAGA AGTGGAAACG 
TTTCCCAATA GTCTAAACTC TCTGGCCCAA GGAAGCATGG AACTCGTTCA AAACATGCTA 
ATGCAGGTTA TGAAGAAGCA CCCAGAAGCA CGGTGGTTCC ACATTGGTGC CGATGAGGTG 
CGTGGCTTGG GCGAAAGTGA AGATTCGAAG CGATGGTTAG AAGCCAACAA CGGAGACATG 
GGCAAGCTCT ACCTCAATCA TGTGACTGCA GTTGCAAAGT TTATGACCAA ACAGAAGCAA 
AGAATCAAGC TGATCTTGTG GGACGACATG TTCAGGAAAT TTAGCCCTGA CACTATAAAA 
GAATCAGGTT TGCAAGATCT TGCTTCACCC ATGATATGGA ACTACCATGC AAACTTGAAC 
GTGAAGGACA TTGGTAAGTA TATCTCCAAG TATGAGCAGG CTGGATTTCG TGGCGTGTGG 
TTTGCTAGTG CTTTCAAAGG AGCATCTGGG ATCGACCAGA TCTGGACTCC AATTGATCAC 
CATTTAAAAA ACCATATACA GTGGCAGGAA GTCATCGCAT CCATGCCGCA GTACAAGTCA 
GTGAGATTTC AAGGCATTGC ACTGACTGGC TGGCAAAGGT ATGAGCATCA CACAGTGCTG 
TGCGAGCTTC TCCCGGTGGC CATTCCTTCT CTCACTGTCT GTCTGCAGAC ACTGAAATAT 
GGTGCATTTA ATGCTAAGGC CCAGGGTGAG GTCCAGCGCA TGCTTGGCTG TGAAATTCAG 
CTCGAAAAAA ATGACTGTAA GGGTGCATCC TTTCCTGGAT CAGACATTTA TGAAATGGTG 
AAGAAGATCC ACACCAACCT TCAGAGTTCA ATTATAAACC TCAAGCAAAA CTATCACGTC 
AAAGGATCAT TCAGCTTTTA TCATCGCAAA CGCAACTTTG CCAACCCTCG AAATCTTGGA 
TTTTTCAAAG ACGATCTGAA AAAAATGATG GATGAATGGG ATTCCTTTTT GCTGACTTTT 
CGGGAAAGCA TGGAGGCGAT TTTTTACCCG GACACTTTAG AGGAGTGGTT GGAAGAGAAC 
GTGAATGAAC ACATTGAAAC GCTTCACAAC ATGGTGGTTG ATGTAGAGCG GATCATTAAA 
CTCAACGGAC AGCCAAAGGT GGACCCCTTG GAAAATAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163933.1
CDS	401..2122
Translation	MSSQKLIRLIIGSLVIVAMVKLFFNPRGSVKSKSEKTHQIIQGGNFWASQRIPLIKVVENKQDTVKTEQQPKVEQEESEKIQKKQEQLKPEVPEKTDPLKIVHLDLKGAPPKVKYLEQLFPLLSSLGANGILLEYEDMFPYEGDLEILKSSFAYSPEDIEEIKSLAKRHNLELIPLVQVFGHMEFVLKHEKYFKLREVETFPNSLNSLAQGSMELVQNMLMQVMKKHPEARWFHIGADEVRGLGESEDSKRWLEANNGDMGKLYLNHVTAVAKFMTKQKQRIKLILWDDMFRKFSPDTIKESGLQDLASPMIWNYHANLNVKDIGKYISKYEQAGFRGVWFASAFKGASGIDQIWTPIDHHLKNHIQWQEVIASMPQYKSVRFQGIALTGWQRYEHHTVLCELLPVAIPSLTVCLQTLKYGAFNAKAQGEVQRMLGCEIQLEKNDCKGASFPGSDIYEMVKKIHTNLQSSIINLKQNYHVKGSFSFYHRKRNFANPRNLGFFKDDLKKMMDEWDSFLLTFRESMEAIFYPDTLEEWLEENVNEHIETLHNMVVDVERIIKLNGQPKVDPLENR

Target ORF information:

RefSeq Version	XM_005163876.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio zgc:113333 (zgc:113333), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163876.3

ORF Insert Sequence:

ATGAGCAGCC AAAAGCTTAT TAGGTTAATC ATCGGCTCCT TAGTGATTGT GGCCATGGTG 
AAACTGTTCT TCAACCCCAG AGGAAGTGTG AAGTCTAAAA GTGAAAAGAC CCACCAGATC 
ATACAAGGTG GTAATTTCTG GGCTAGCCAA CGAATCCCAC TGATAAAAGT GGTTGAAAAT 
AAACAGGATA CCGTGAAAAC AGAACAACAG CCTAAAGTTG AGCAAGAGGA AAGTGAGAAG 
ATTCAGAAAA AACAAGAGCA ATTAAAGCCA GAAGTTCCTG AAAAAACAGA TCCACTAAAG 
ATTGTGCATT TAGATCTGAA AGGTGCCCCA CCCAAAGTTA AATACCTTGA GCAGTTATTC 
CCTCTACTGT CATCTTTGGG TGCTAATGGG ATATTGCTGG AATATGAGGA CATGTTTCCC 
TATGAGGGAG ACCTTGAAAT TCTCAAATCA AGCTTTGCAT ACAGTCCGGA AGATATAGAG 
GAAATTAAGT CTCTGGCTAA ACGACACAAT CTGGAACTGA TACCTTTGGT GCAGGTGTTT 
GGACACATGG AGTTCGTCTT AAAACATGAA AAGTATTTTA AATTGAGAGA AGTGGAAACG 
TTTCCCAATA GTCTAAACTC TCTGGCCCAA GGAAGCATGG AACTCGTTCA AAACATGCTA 
ATGCAGGTTA TGAAGAAGCA CCCAGAAGCA CGGTGGTTCC ACATTGGTGC CGATGAGGTG 
CGTGGCTTGG GCGAAAGTGA AGATTCGAAG CGATGGTTAG AAGCCAACAA CGGAGACATG 
GGCAAGCTCT ACCTCAATCA TGTGACTGCA GTTGCAAAGT TTATGACCAA ACAGAAGCAA 
AGAATCAAGC TGATCTTGTG GGACGACATG TTCAGGAAAT TTAGCCCTGA CACTATAAAA 
GAATCAGGTT TGCAAGATCT TGCTTCACCC ATGATATGGA ACTACCATGC AAACTTGAAC 
GTGAAGGACA TTGGTAAGTA TATCTCCAAG TATGAGCAGG CTGGATTTCG TGGCGTGTGG 
TTTGCTAGTG CTTTCAAAGG AGCATCTGGG ATCGACCAGA TCTGGACTCC AATTGATCAC 
CATTTAAAAA ACCATATACA GTGGCAGGAA GTCATCGCAT CCATGCCGCA GTACAAGTCA 
GTGAGATTTC AAGGCATTGC ACTGACTGGC TGGCAAAGGT ATGAGCATCA CACAGTGCTG 
TGCGAGCTTC TCCCGGTGGC CATTCCTTCT CTCACTGTCT GTCTGCAGAC ACTGAAATAT 
GGTGCATTTA ATGCTAAGGC CCAGGGTGAG GTCCAGCGCA TGCTTGGCTG TGAAATTCAG 
CTCGAAAAAA ATGACTGTAA GGGTGCATCC TTTCCTGGAT CAGACATTTA TGAAATGGTG 
AAGAAGATCC ACACCAACCT TCAGAGTTCA ATTATAAACC TCAAGCAAAA CTATCACGTC 
AAAGGATCAT TCAGCTTTTA TCATCGCAAA CGCAACTTTG CCAACCCTCG AAATCTTGGA 
TTTTTCAAAG ACGATCTGAA AAAAATGATG GATGAATGGG ATTCCTTTTT GCTGACTTTT 
CGGGAAAGCA TGGAGGCGAT TTTTTACCCG GACACTTTAG AGGAGTGGTT GGAAGAGAAC 
GTGAATGAAC ACATTGAAAC GCTTCACAAC ATGGTGGTTG ATGTAGAGCG GATCATTAAA 
CTCAACGGAC AGCCAAAGGT GGACCCCTTG GAAAATAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

zgc:113333 ( XM_005163875.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005163932.1 Danio rerio zgc:113333 (zgc:113333), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa08151
Clone ID Related Accession (Same CDS sequence)	XM_005163875.3 , XM_009299336.2 , XM_005163876.3 , XM_005163875.4 , XM_005163876.4 , XM_009299336.3
Accession Version	XM_005163875.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	hexosaminidase D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005163875.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGCC AAAAGCTTAT TAGGTTAATC ATCGGCTCCT TAGTGATTGT GGCCATGGTG 
AAACTGTTCT TCAACCCCAG AGGAAGTGTG AAGTCTAAAA GTGAAAAGAC CCACCAGATC 
ATACAAGGTG GTAATTTCTG GGCTAGCCAA CGAATCCCAC TGATAAAAGT GGTTGAAAAT 
AAACAGGATA CCGTGAAAAC AGAACAACAG CCTAAAGTTG AGCAAGAGGA AAGTGAGAAG 
ATTCAGAAAA AACAAGAGCA ATTAAAGCCA GAAGTTCCTG AAAAAACAGA TCCACTAAAG 
ATTGTGCATT TAGATCTGAA AGGTGCCCCA CCCAAAGTTA AATACCTTGA GCAGTTATTC 
CCTCTACTGT CATCTTTGGG TGCTAATGGG ATATTGCTGG AATATGAGGA CATGTTTCCC 
TATGAGGGAG ACCTTGAAAT TCTCAAATCA AGCTTTGCAT ACAGTCCGGA AGATATAGAG 
GAAATTAAGT CTCTGGCTAA ACGACACAAT CTGGAACTGA TACCTTTGGT GCAGGTGTTT 
GGACACATGG AGTTCGTCTT AAAACATGAA AAGTATTTTA AATTGAGAGA AGTGGAAACG 
TTTCCCAATA GTCTAAACTC TCTGGCCCAA GGAAGCATGG AACTCGTTCA AAACATGCTA 
ATGCAGGTTA TGAAGAAGCA CCCAGAAGCA CGGTGGTTCC ACATTGGTGC CGATGAGGTG 
CGTGGCTTGG GCGAAAGTGA AGATTCGAAG CGATGGTTAG AAGCCAACAA CGGAGACATG 
GGCAAGCTCT ACCTCAATCA TGTGACTGCA GTTGCAAAGT TTATGACCAA ACAGAAGCAA 
AGAATCAAGC TGATCTTGTG GGACGACATG TTCAGGAAAT TTAGCCCTGA CACTATAAAA 
GAATCAGGTT TGCAAGATCT TGCTTCACCC ATGATATGGA ACTACCATGC AAACTTGAAC 
GTGAAGGACA TTGGTAAGTA TATCTCCAAG TATGAGCAGG CTGGATTTCG TGGCGTGTGG 
TTTGCTAGTG CTTTCAAAGG AGCATCTGGG ATCGACCAGA TCTGGACTCC AATTGATCAC 
CATTTAAAAA ACCATATACA GTGGCAGGAA GTCATCGCAT CCATGCCGCA GTACAAGTCA 
GTGAGATTTC AAGGCATTGC ACTGACTGGC TGGCAAAGGT ATGAGCATCA CACAGTGCTG 
TGCGAGCTTC TCCCGGTGGC CATTCCTTCT CTCACTGTCT GTCTGCAGAC ACTGAAATAT 
GGTGCATTTA ATGCTAAGGC CCAGGGTGAG GTCCAGCGCA TGCTTGGCTG TGAAATTCAG 
CTCGAAAAAA ATGACTGTAA GGGTGCATCC TTTCCTGGAT CAGACATTTA TGAAATGGTG 
AAGAAGATCC ACACCAACCT TCAGAGTTCA ATTATAAACC TCAAGCAAAA CTATCACGTC 
AAAGGATCAT TCAGCTTTTA TCATCGCAAA CGCAACTTTG CCAACCCTCG AAATCTTGGA 
TTTTTCAAAG ACGATCTGAA AAAAATGATG GATGAATGGG ATTCCTTTTT GCTGACTTTT 
CGGGAAAGCA TGGAGGCGAT TTTTTACCCG GACACTTTAG AGGAGTGGTT GGAAGAGAAC 
GTGAATGAAC ACATTGAAAC GCTTCACAAC ATGGTGGTTG ATGTAGAGCG GATCATTAAA 
CTCAACGGAC AGCCAAAGGT GGACCCCTTG GAAAATAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163932.1
CDS	517..2238
Translation	MSSQKLIRLIIGSLVIVAMVKLFFNPRGSVKSKSEKTHQIIQGGNFWASQRIPLIKVVENKQDTVKTEQQPKVEQEESEKIQKKQEQLKPEVPEKTDPLKIVHLDLKGAPPKVKYLEQLFPLLSSLGANGILLEYEDMFPYEGDLEILKSSFAYSPEDIEEIKSLAKRHNLELIPLVQVFGHMEFVLKHEKYFKLREVETFPNSLNSLAQGSMELVQNMLMQVMKKHPEARWFHIGADEVRGLGESEDSKRWLEANNGDMGKLYLNHVTAVAKFMTKQKQRIKLILWDDMFRKFSPDTIKESGLQDLASPMIWNYHANLNVKDIGKYISKYEQAGFRGVWFASAFKGASGIDQIWTPIDHHLKNHIQWQEVIASMPQYKSVRFQGIALTGWQRYEHHTVLCELLPVAIPSLTVCLQTLKYGAFNAKAQGEVQRMLGCEIQLEKNDCKGASFPGSDIYEMVKKIHTNLQSSIINLKQNYHVKGSFSFYHRKRNFANPRNLGFFKDDLKKMMDEWDSFLLTFRESMEAIFYPDTLEEWLEENVNEHIETLHNMVVDVERIIKLNGQPKVDPLENR

Target ORF information:

RefSeq Version	XM_005163875.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio zgc:113333 (zgc:113333), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163875.4

ORF Insert Sequence:

ATGAGCAGCC AAAAGCTTAT TAGGTTAATC ATCGGCTCCT TAGTGATTGT GGCCATGGTG 
AAACTGTTCT TCAACCCCAG AGGAAGTGTG AAGTCTAAAA GTGAAAAGAC CCACCAGATC 
ATACAAGGTG GTAATTTCTG GGCTAGCCAA CGAATCCCAC TGATAAAAGT GGTTGAAAAT 
AAACAGGATA CCGTGAAAAC AGAACAACAG CCTAAAGTTG AGCAAGAGGA AAGTGAGAAG 
ATTCAGAAAA AACAAGAGCA ATTAAAGCCA GAAGTTCCTG AAAAAACAGA TCCACTAAAG 
ATTGTGCATT TAGATCTGAA AGGTGCCCCA CCCAAAGTTA AATACCTTGA GCAGTTATTC 
CCTCTACTGT CATCTTTGGG TGCTAATGGG ATATTGCTGG AATATGAGGA CATGTTTCCC 
TATGAGGGAG ACCTTGAAAT TCTCAAATCA AGCTTTGCAT ACAGTCCGGA AGATATAGAG 
GAAATTAAGT CTCTGGCTAA ACGACACAAT CTGGAACTGA TACCTTTGGT GCAGGTGTTT 
GGACACATGG AGTTCGTCTT AAAACATGAA AAGTATTTTA AATTGAGAGA AGTGGAAACG 
TTTCCCAATA GTCTAAACTC TCTGGCCCAA GGAAGCATGG AACTCGTTCA AAACATGCTA 
ATGCAGGTTA TGAAGAAGCA CCCAGAAGCA CGGTGGTTCC ACATTGGTGC CGATGAGGTG 
CGTGGCTTGG GCGAAAGTGA AGATTCGAAG CGATGGTTAG AAGCCAACAA CGGAGACATG 
GGCAAGCTCT ACCTCAATCA TGTGACTGCA GTTGCAAAGT TTATGACCAA ACAGAAGCAA 
AGAATCAAGC TGATCTTGTG GGACGACATG TTCAGGAAAT TTAGCCCTGA CACTATAAAA 
GAATCAGGTT TGCAAGATCT TGCTTCACCC ATGATATGGA ACTACCATGC AAACTTGAAC 
GTGAAGGACA TTGGTAAGTA TATCTCCAAG TATGAGCAGG CTGGATTTCG TGGCGTGTGG 
TTTGCTAGTG CTTTCAAAGG AGCATCTGGG ATCGACCAGA TCTGGACTCC AATTGATCAC 
CATTTAAAAA ACCATATACA GTGGCAGGAA GTCATCGCAT CCATGCCGCA GTACAAGTCA 
GTGAGATTTC AAGGCATTGC ACTGACTGGC TGGCAAAGGT ATGAGCATCA CACAGTGCTG 
TGCGAGCTTC TCCCGGTGGC CATTCCTTCT CTCACTGTCT GTCTGCAGAC ACTGAAATAT 
GGTGCATTTA ATGCTAAGGC CCAGGGTGAG GTCCAGCGCA TGCTTGGCTG TGAAATTCAG 
CTCGAAAAAA ATGACTGTAA GGGTGCATCC TTTCCTGGAT CAGACATTTA TGAAATGGTG 
AAGAAGATCC ACACCAACCT TCAGAGTTCA ATTATAAACC TCAAGCAAAA CTATCACGTC 
AAAGGATCAT TCAGCTTTTA TCATCGCAAA CGCAACTTTG CCAACCCTCG AAATCTTGGA 
TTTTTCAAAG ACGATCTGAA AAAAATGATG GATGAATGGG ATTCCTTTTT GCTGACTTTT 
CGGGAAAGCA TGGAGGCGAT TTTTTACCCG GACACTTTAG AGGAGTGGTT GGAAGAGAAC 
GTGAATGAAC ACATTGAAAC GCTTCACAAC ATGGTGGTTG ATGTAGAGCG GATCATTAAA 
CTCAACGGAC AGCCAAAGGT GGACCCCTTG GAAAATAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

zgc:113333 ( XM_005163876.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005163933.1 Danio rerio zgc:113333 (zgc:113333), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa08151
Clone ID Related Accession (Same CDS sequence)	XM_005163875.3 , XM_009299336.2 , XM_005163876.3 , XM_005163875.4 , XM_005163876.4 , XM_009299336.3
Accession Version	XM_005163876.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	hexosaminidase D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005163876.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGCC AAAAGCTTAT TAGGTTAATC ATCGGCTCCT TAGTGATTGT GGCCATGGTG 
AAACTGTTCT TCAACCCCAG AGGAAGTGTG AAGTCTAAAA GTGAAAAGAC CCACCAGATC 
ATACAAGGTG GTAATTTCTG GGCTAGCCAA CGAATCCCAC TGATAAAAGT GGTTGAAAAT 
AAACAGGATA CCGTGAAAAC AGAACAACAG CCTAAAGTTG AGCAAGAGGA AAGTGAGAAG 
ATTCAGAAAA AACAAGAGCA ATTAAAGCCA GAAGTTCCTG AAAAAACAGA TCCACTAAAG 
ATTGTGCATT TAGATCTGAA AGGTGCCCCA CCCAAAGTTA AATACCTTGA GCAGTTATTC 
CCTCTACTGT CATCTTTGGG TGCTAATGGG ATATTGCTGG AATATGAGGA CATGTTTCCC 
TATGAGGGAG ACCTTGAAAT TCTCAAATCA AGCTTTGCAT ACAGTCCGGA AGATATAGAG 
GAAATTAAGT CTCTGGCTAA ACGACACAAT CTGGAACTGA TACCTTTGGT GCAGGTGTTT 
GGACACATGG AGTTCGTCTT AAAACATGAA AAGTATTTTA AATTGAGAGA AGTGGAAACG 
TTTCCCAATA GTCTAAACTC TCTGGCCCAA GGAAGCATGG AACTCGTTCA AAACATGCTA 
ATGCAGGTTA TGAAGAAGCA CCCAGAAGCA CGGTGGTTCC ACATTGGTGC CGATGAGGTG 
CGTGGCTTGG GCGAAAGTGA AGATTCGAAG CGATGGTTAG AAGCCAACAA CGGAGACATG 
GGCAAGCTCT ACCTCAATCA TGTGACTGCA GTTGCAAAGT TTATGACCAA ACAGAAGCAA 
AGAATCAAGC TGATCTTGTG GGACGACATG TTCAGGAAAT TTAGCCCTGA CACTATAAAA 
GAATCAGGTT TGCAAGATCT TGCTTCACCC ATGATATGGA ACTACCATGC AAACTTGAAC 
GTGAAGGACA TTGGTAAGTA TATCTCCAAG TATGAGCAGG CTGGATTTCG TGGCGTGTGG 
TTTGCTAGTG CTTTCAAAGG AGCATCTGGG ATCGACCAGA TCTGGACTCC AATTGATCAC 
CATTTAAAAA ACCATATACA GTGGCAGGAA GTCATCGCAT CCATGCCGCA GTACAAGTCA 
GTGAGATTTC AAGGCATTGC ACTGACTGGC TGGCAAAGGT ATGAGCATCA CACAGTGCTG 
TGCGAGCTTC TCCCGGTGGC CATTCCTTCT CTCACTGTCT GTCTGCAGAC ACTGAAATAT 
GGTGCATTTA ATGCTAAGGC CCAGGGTGAG GTCCAGCGCA TGCTTGGCTG TGAAATTCAG 
CTCGAAAAAA ATGACTGTAA GGGTGCATCC TTTCCTGGAT CAGACATTTA TGAAATGGTG 
AAGAAGATCC ACACCAACCT TCAGAGTTCA ATTATAAACC TCAAGCAAAA CTATCACGTC 
AAAGGATCAT TCAGCTTTTA TCATCGCAAA CGCAACTTTG CCAACCCTCG AAATCTTGGA 
TTTTTCAAAG ACGATCTGAA AAAAATGATG GATGAATGGG ATTCCTTTTT GCTGACTTTT 
CGGGAAAGCA TGGAGGCGAT TTTTTACCCG GACACTTTAG AGGAGTGGTT GGAAGAGAAC 
GTGAATGAAC ACATTGAAAC GCTTCACAAC ATGGTGGTTG ATGTAGAGCG GATCATTAAA 
CTCAACGGAC AGCCAAAGGT GGACCCCTTG GAAAATAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163933.1
CDS	410..2131
Translation	MSSQKLIRLIIGSLVIVAMVKLFFNPRGSVKSKSEKTHQIIQGGNFWASQRIPLIKVVENKQDTVKTEQQPKVEQEESEKIQKKQEQLKPEVPEKTDPLKIVHLDLKGAPPKVKYLEQLFPLLSSLGANGILLEYEDMFPYEGDLEILKSSFAYSPEDIEEIKSLAKRHNLELIPLVQVFGHMEFVLKHEKYFKLREVETFPNSLNSLAQGSMELVQNMLMQVMKKHPEARWFHIGADEVRGLGESEDSKRWLEANNGDMGKLYLNHVTAVAKFMTKQKQRIKLILWDDMFRKFSPDTIKESGLQDLASPMIWNYHANLNVKDIGKYISKYEQAGFRGVWFASAFKGASGIDQIWTPIDHHLKNHIQWQEVIASMPQYKSVRFQGIALTGWQRYEHHTVLCELLPVAIPSLTVCLQTLKYGAFNAKAQGEVQRMLGCEIQLEKNDCKGASFPGSDIYEMVKKIHTNLQSSIINLKQNYHVKGSFSFYHRKRNFANPRNLGFFKDDLKKMMDEWDSFLLTFRESMEAIFYPDTLEEWLEENVNEHIETLHNMVVDVERIIKLNGQPKVDPLENR

Target ORF information:

RefSeq Version	XM_005163876.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio zgc:113333 (zgc:113333), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163876.4

ORF Insert Sequence:

ATGAGCAGCC AAAAGCTTAT TAGGTTAATC ATCGGCTCCT TAGTGATTGT GGCCATGGTG 
AAACTGTTCT TCAACCCCAG AGGAAGTGTG AAGTCTAAAA GTGAAAAGAC CCACCAGATC 
ATACAAGGTG GTAATTTCTG GGCTAGCCAA CGAATCCCAC TGATAAAAGT GGTTGAAAAT 
AAACAGGATA CCGTGAAAAC AGAACAACAG CCTAAAGTTG AGCAAGAGGA AAGTGAGAAG 
ATTCAGAAAA AACAAGAGCA ATTAAAGCCA GAAGTTCCTG AAAAAACAGA TCCACTAAAG 
ATTGTGCATT TAGATCTGAA AGGTGCCCCA CCCAAAGTTA AATACCTTGA GCAGTTATTC 
CCTCTACTGT CATCTTTGGG TGCTAATGGG ATATTGCTGG AATATGAGGA CATGTTTCCC 
TATGAGGGAG ACCTTGAAAT TCTCAAATCA AGCTTTGCAT ACAGTCCGGA AGATATAGAG 
GAAATTAAGT CTCTGGCTAA ACGACACAAT CTGGAACTGA TACCTTTGGT GCAGGTGTTT 
GGACACATGG AGTTCGTCTT AAAACATGAA AAGTATTTTA AATTGAGAGA AGTGGAAACG 
TTTCCCAATA GTCTAAACTC TCTGGCCCAA GGAAGCATGG AACTCGTTCA AAACATGCTA 
ATGCAGGTTA TGAAGAAGCA CCCAGAAGCA CGGTGGTTCC ACATTGGTGC CGATGAGGTG 
CGTGGCTTGG GCGAAAGTGA AGATTCGAAG CGATGGTTAG AAGCCAACAA CGGAGACATG 
GGCAAGCTCT ACCTCAATCA TGTGACTGCA GTTGCAAAGT TTATGACCAA ACAGAAGCAA 
AGAATCAAGC TGATCTTGTG GGACGACATG TTCAGGAAAT TTAGCCCTGA CACTATAAAA 
GAATCAGGTT TGCAAGATCT TGCTTCACCC ATGATATGGA ACTACCATGC AAACTTGAAC 
GTGAAGGACA TTGGTAAGTA TATCTCCAAG TATGAGCAGG CTGGATTTCG TGGCGTGTGG 
TTTGCTAGTG CTTTCAAAGG AGCATCTGGG ATCGACCAGA TCTGGACTCC AATTGATCAC 
CATTTAAAAA ACCATATACA GTGGCAGGAA GTCATCGCAT CCATGCCGCA GTACAAGTCA 
GTGAGATTTC AAGGCATTGC ACTGACTGGC TGGCAAAGGT ATGAGCATCA CACAGTGCTG 
TGCGAGCTTC TCCCGGTGGC CATTCCTTCT CTCACTGTCT GTCTGCAGAC ACTGAAATAT 
GGTGCATTTA ATGCTAAGGC CCAGGGTGAG GTCCAGCGCA TGCTTGGCTG TGAAATTCAG 
CTCGAAAAAA ATGACTGTAA GGGTGCATCC TTTCCTGGAT CAGACATTTA TGAAATGGTG 
AAGAAGATCC ACACCAACCT TCAGAGTTCA ATTATAAACC TCAAGCAAAA CTATCACGTC 
AAAGGATCAT TCAGCTTTTA TCATCGCAAA CGCAACTTTG CCAACCCTCG AAATCTTGGA 
TTTTTCAAAG ACGATCTGAA AAAAATGATG GATGAATGGG ATTCCTTTTT GCTGACTTTT 
CGGGAAAGCA TGGAGGCGAT TTTTTACCCG GACACTTTAG AGGAGTGGTT GGAAGAGAAC 
GTGAATGAAC ACATTGAAAC GCTTCACAAC ATGGTGGTTG ATGTAGAGCG GATCATTAAA 
CTCAACGGAC AGCCAAAGGT GGACCCCTTG GAAAATAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

zgc:113333 ( XM_009299336.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009297611.1 Danio rerio zgc:113333 (zgc:113333), transcript variant X2, mRNA.

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CloneID	ODa08151
Clone ID Related Accession (Same CDS sequence)	XM_005163875.3 , XM_009299336.2 , XM_005163876.3 , XM_005163875.4 , XM_005163876.4 , XM_009299336.3
Accession Version	XM_009299336.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	hexosaminidase D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009299336.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGCC AAAAGCTTAT TAGGTTAATC ATCGGCTCCT TAGTGATTGT GGCCATGGTG 
AAACTGTTCT TCAACCCCAG AGGAAGTGTG AAGTCTAAAA GTGAAAAGAC CCACCAGATC 
ATACAAGGTG GTAATTTCTG GGCTAGCCAA CGAATCCCAC TGATAAAAGT GGTTGAAAAT 
AAACAGGATA CCGTGAAAAC AGAACAACAG CCTAAAGTTG AGCAAGAGGA AAGTGAGAAG 
ATTCAGAAAA AACAAGAGCA ATTAAAGCCA GAAGTTCCTG AAAAAACAGA TCCACTAAAG 
ATTGTGCATT TAGATCTGAA AGGTGCCCCA CCCAAAGTTA AATACCTTGA GCAGTTATTC 
CCTCTACTGT CATCTTTGGG TGCTAATGGG ATATTGCTGG AATATGAGGA CATGTTTCCC 
TATGAGGGAG ACCTTGAAAT TCTCAAATCA AGCTTTGCAT ACAGTCCGGA AGATATAGAG 
GAAATTAAGT CTCTGGCTAA ACGACACAAT CTGGAACTGA TACCTTTGGT GCAGGTGTTT 
GGACACATGG AGTTCGTCTT AAAACATGAA AAGTATTTTA AATTGAGAGA AGTGGAAACG 
TTTCCCAATA GTCTAAACTC TCTGGCCCAA GGAAGCATGG AACTCGTTCA AAACATGCTA 
ATGCAGGTTA TGAAGAAGCA CCCAGAAGCA CGGTGGTTCC ACATTGGTGC CGATGAGGTG 
CGTGGCTTGG GCGAAAGTGA AGATTCGAAG CGATGGTTAG AAGCCAACAA CGGAGACATG 
GGCAAGCTCT ACCTCAATCA TGTGACTGCA GTTGCAAAGT TTATGACCAA ACAGAAGCAA 
AGAATCAAGC TGATCTTGTG GGACGACATG TTCAGGAAAT TTAGCCCTGA CACTATAAAA 
GAATCAGGTT TGCAAGATCT TGCTTCACCC ATGATATGGA ACTACCATGC AAACTTGAAC 
GTGAAGGACA TTGGTAAGTA TATCTCCAAG TATGAGCAGG CTGGATTTCG TGGCGTGTGG 
TTTGCTAGTG CTTTCAAAGG AGCATCTGGG ATCGACCAGA TCTGGACTCC AATTGATCAC 
CATTTAAAAA ACCATATACA GTGGCAGGAA GTCATCGCAT CCATGCCGCA GTACAAGTCA 
GTGAGATTTC AAGGCATTGC ACTGACTGGC TGGCAAAGGT ATGAGCATCA CACAGTGCTG 
TGCGAGCTTC TCCCGGTGGC CATTCCTTCT CTCACTGTCT GTCTGCAGAC ACTGAAATAT 
GGTGCATTTA ATGCTAAGGC CCAGGGTGAG GTCCAGCGCA TGCTTGGCTG TGAAATTCAG 
CTCGAAAAAA ATGACTGTAA GGGTGCATCC TTTCCTGGAT CAGACATTTA TGAAATGGTG 
AAGAAGATCC ACACCAACCT TCAGAGTTCA ATTATAAACC TCAAGCAAAA CTATCACGTC 
AAAGGATCAT TCAGCTTTTA TCATCGCAAA CGCAACTTTG CCAACCCTCG AAATCTTGGA 
TTTTTCAAAG ACGATCTGAA AAAAATGATG GATGAATGGG ATTCCTTTTT GCTGACTTTT 
CGGGAAAGCA TGGAGGCGAT TTTTTACCCG GACACTTTAG AGGAGTGGTT GGAAGAGAAC 
GTGAATGAAC ACATTGAAAC GCTTCACAAC ATGGTGGTTG ATGTAGAGCG GATCATTAAA 
CTCAACGGAC AGCCAAAGGT GGACCCCTTG GAAAATAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297611.1
CDS	548..2269
Translation	MSSQKLIRLIIGSLVIVAMVKLFFNPRGSVKSKSEKTHQIIQGGNFWASQRIPLIKVVENKQDTVKTEQQPKVEQEESEKIQKKQEQLKPEVPEKTDPLKIVHLDLKGAPPKVKYLEQLFPLLSSLGANGILLEYEDMFPYEGDLEILKSSFAYSPEDIEEIKSLAKRHNLELIPLVQVFGHMEFVLKHEKYFKLREVETFPNSLNSLAQGSMELVQNMLMQVMKKHPEARWFHIGADEVRGLGESEDSKRWLEANNGDMGKLYLNHVTAVAKFMTKQKQRIKLILWDDMFRKFSPDTIKESGLQDLASPMIWNYHANLNVKDIGKYISKYEQAGFRGVWFASAFKGASGIDQIWTPIDHHLKNHIQWQEVIASMPQYKSVRFQGIALTGWQRYEHHTVLCELLPVAIPSLTVCLQTLKYGAFNAKAQGEVQRMLGCEIQLEKNDCKGASFPGSDIYEMVKKIHTNLQSSIINLKQNYHVKGSFSFYHRKRNFANPRNLGFFKDDLKKMMDEWDSFLLTFRESMEAIFYPDTLEEWLEENVNEHIETLHNMVVDVERIIKLNGQPKVDPLENR

Target ORF information:

RefSeq Version	XM_009299336.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio zgc:113333 (zgc:113333), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299336.3

ORF Insert Sequence:

ATGAGCAGCC AAAAGCTTAT TAGGTTAATC ATCGGCTCCT TAGTGATTGT GGCCATGGTG 
AAACTGTTCT TCAACCCCAG AGGAAGTGTG AAGTCTAAAA GTGAAAAGAC CCACCAGATC 
ATACAAGGTG GTAATTTCTG GGCTAGCCAA CGAATCCCAC TGATAAAAGT GGTTGAAAAT 
AAACAGGATA CCGTGAAAAC AGAACAACAG CCTAAAGTTG AGCAAGAGGA AAGTGAGAAG 
ATTCAGAAAA AACAAGAGCA ATTAAAGCCA GAAGTTCCTG AAAAAACAGA TCCACTAAAG 
ATTGTGCATT TAGATCTGAA AGGTGCCCCA CCCAAAGTTA AATACCTTGA GCAGTTATTC 
CCTCTACTGT CATCTTTGGG TGCTAATGGG ATATTGCTGG AATATGAGGA CATGTTTCCC 
TATGAGGGAG ACCTTGAAAT TCTCAAATCA AGCTTTGCAT ACAGTCCGGA AGATATAGAG 
GAAATTAAGT CTCTGGCTAA ACGACACAAT CTGGAACTGA TACCTTTGGT GCAGGTGTTT 
GGACACATGG AGTTCGTCTT AAAACATGAA AAGTATTTTA AATTGAGAGA AGTGGAAACG 
TTTCCCAATA GTCTAAACTC TCTGGCCCAA GGAAGCATGG AACTCGTTCA AAACATGCTA 
ATGCAGGTTA TGAAGAAGCA CCCAGAAGCA CGGTGGTTCC ACATTGGTGC CGATGAGGTG 
CGTGGCTTGG GCGAAAGTGA AGATTCGAAG CGATGGTTAG AAGCCAACAA CGGAGACATG 
GGCAAGCTCT ACCTCAATCA TGTGACTGCA GTTGCAAAGT TTATGACCAA ACAGAAGCAA 
AGAATCAAGC TGATCTTGTG GGACGACATG TTCAGGAAAT TTAGCCCTGA CACTATAAAA 
GAATCAGGTT TGCAAGATCT TGCTTCACCC ATGATATGGA ACTACCATGC AAACTTGAAC 
GTGAAGGACA TTGGTAAGTA TATCTCCAAG TATGAGCAGG CTGGATTTCG TGGCGTGTGG 
TTTGCTAGTG CTTTCAAAGG AGCATCTGGG ATCGACCAGA TCTGGACTCC AATTGATCAC 
CATTTAAAAA ACCATATACA GTGGCAGGAA GTCATCGCAT CCATGCCGCA GTACAAGTCA 
GTGAGATTTC AAGGCATTGC ACTGACTGGC TGGCAAAGGT ATGAGCATCA CACAGTGCTG 
TGCGAGCTTC TCCCGGTGGC CATTCCTTCT CTCACTGTCT GTCTGCAGAC ACTGAAATAT 
GGTGCATTTA ATGCTAAGGC CCAGGGTGAG GTCCAGCGCA TGCTTGGCTG TGAAATTCAG 
CTCGAAAAAA ATGACTGTAA GGGTGCATCC TTTCCTGGAT CAGACATTTA TGAAATGGTG 
AAGAAGATCC ACACCAACCT TCAGAGTTCA ATTATAAACC TCAAGCAAAA CTATCACGTC 
AAAGGATCAT TCAGCTTTTA TCATCGCAAA CGCAACTTTG CCAACCCTCG AAATCTTGGA 
TTTTTCAAAG ACGATCTGAA AAAAATGATG GATGAATGGG ATTCCTTTTT GCTGACTTTT 
CGGGAAAGCA TGGAGGCGAT TTTTTACCCG GACACTTTAG AGGAGTGGTT GGAAGAGAAC 
GTGAATGAAC ACATTGAAAC GCTTCACAAC ATGGTGGTTG ATGTAGAGCG GATCATTAAA 
CTCAACGGAC AGCCAAAGGT GGACCCCTTG GAAAATAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

A live zebrafish-based screening system for human nuclear receptor ligand and cofactor discovery.
PloS one5(3)e9797(2010 Mar)
Tiefenbach J,Moll PR,Nelson MR,Hu C,Baev L,Kislinger T,Krause HM

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zgc:113333 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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