brox cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	brox
Entrez Gene ID	492786
Full Name	BRO1 domain and CAAX motif containing
Synonyms	si:ch211-14a11.3,zgc:101706
General protein information	Preferred Names BRO1 domain and CAAX motif containing Names BRO1 domain-containing protein BROX BRO1 domain- and CAAX motif-containing protein
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001007427.2	NP_001007428.2	BRO1 domain-containing protein BROX

Pathways from BioSystems

Homologies

Homo sapiens (human)	BROX	NP_653296.2
	BROX	XP_001099998.2
Mus musculus (house mouse)	Brox	NP_082137.1
Rattus norvegicus (Norway rat)	Brox	NP_001020840.1
Gallus gallus (chicken)	BROX	XP_004935363.1
Caenorhabditis elegans (roundworm)	B0507.2	NP_505261.2
Pan troglodytes (chimpanzee)	BROX	NP_001233322.1
Canis lupus familiaris (dog)	BROX	XP_536151.3
Bos taurus (cattle)	BROX	NP_001192823.1
Danio rerio (zebrafish)	brox	NP_001007428.1
Xenopus tropicalis (tropical clawed frog)	brox	NP_001096412.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Effects of hypothermia on gene expression in zebrafish gills: upregulation in differentiation and function of ionocytes as compensatory responses.
Chou MY, Hsiao CD, Chen SC, Chen IW, Liu ST, Hwang PP
The Journal of experimental biology211(Pt 19)3077-84(2008 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following brox gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the brox cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa41405	NM_001007427.2 Latest version!	Danio rerio BRO1 domain and CAAX motif containing (brox), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

brox ( NM_001007427.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001007428.2 Danio rerio BRO1 domain and CAAX motif containing (brox), mRNA.

Price & Availability↑

CloneID	ODa41405
Clone ID Related Accession (Same CDS sequence)	NM_001007427.2
Accession Version	NM_001007427.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Danio rerio(zebrafish)
Product	BRO1 domain-containing protein BROX
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX936461.17 and DT073728.1. On or before Sep 27, 2016 this sequence version replaced XM_009295011.2, NM_001007427.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## CDS exon combination :: BC085420.1, GDQH01005773.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGACTCACT GGTTCCACAG AAACCCTCTG AAAGCCACCG CCCCAGTGTC CTTCAATCTG 
TATGGCGTGG CATCCAGCCC TGCGGCCAAT AAGATCTGCA ATGACCTGCG AACAGCACGC 
GCTCGACTGC TGGACATGTT CACAGATGCC ACATGTTCTC CAGACACCCT GAAGAAATCC 
AGCGATGAGT ATTTTGCTCT TTTACAGGGT TTCATTTTAC CGCTTGATGG CACTACGCAA 
GAGAACAAGC TCAGATTCAT CCAGAATTTC AAATGGACCG ACACTTTACA GGGAAATATA 
GCCAGTGCGC AGCAGGATGC CGTGTTTGAG CTGGTGTCTA TGGCCTTTAA TGTGGCCCTG 
TGGTACACCA AGTTTGCCTC TCGATTGGCT GGAAAGGAGA ACATCACTGA GGAGGAGGCC 
AAAGATGTTC ACAAGAGTTT GAAAATTGCT GCTGGGATAT TTAAAACCCT TAAGGAGACT 
CATATTCCTC GGCTCATCAC TCCAGCAGAG AAGGGTCGAG ATCTGGAGCC GCGAGTTATC 
GACACGTACA TCGTCCAGAG TCAAGCAGAG GCTCAGGAAG TGACCATCGC CAGAGCCATT 
GAGCTGAAGC ACAATGCCTC CCTCATTGCA GCGCTGGCCT TCGAGACTGC CAACTTCTAC 
CAGAAAGCCG ATCACACTCT GAACACTTTG GATCCAGAAT GCAGCAGTAA ATGGAAGAAA 
TACCTGCAGC TGAAGCAGCA CTTCTACATG GCTTACGCTC ACTGCTATCA TGGACAGACT 
CTTCTAGCGG GCGATAAATG TGGAGAGGCC ATAAGATCCC TGCAGGAAGC TGAGAAATGT 
TACTCTCGCG CTGAAGTCTT GTGTAAAGAG TACCGTCAGA TGAAGGGTCC CGGCAGCACC 
GCCAAACCCT CCGAACAGCT CTTCTTCAAG AAACTGGGCG CCGTCATCAA GAACACCTTG 
GAGAAATGCC AGAGAGAGAA CGGCTTCATA TACTTCCACA AGGTTCCGGC TGAAGCTCCG 
GCTCTGGAGT TGAAGGCCAG TTATGGTTTG GCTGAACCCA CTGCGTTCGA GCTCCCGCCG 
CTCAGCGCTC AGTGCACCCC TGAAGTCTAC GCCACCTTTG ACCTGACCAG AGGACACAAA 
GACGACAAGG CGAAAGCGAA GCCTGAAGAG GAGATCAAGC CGGTGAAAGA ACCAGATCTA 
AAGCCGCAGA AGGACACAGG CTGCGTTGTC TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001007428.2
CDS	102..1337
Translation	MTHWFHRNPLKATAPVSFNLYGVASSPAANKICNDLRTARARLLDMFTDATCSPDTLKKSSDEYFALLQGFILPLDGTTQENKLRFIQNFKWTDTLQGNIASAQQDAVFELVSMAFNVALWYTKFASRLAGKENITEEEAKDVHKSLKIAAGIFKTLKETHIPRLITPAEKGRDLEPRVIDTYIVQSQAEAQEVTIARAIELKHNASLIAALAFETANFYQKADHTLNTLDPECSSKWKKYLQLKQHFYMAYAHCYHGQTLLAGDKCGEAIRSLQEAEKCYSRAEVLCKEYRQMKGPGSTAKPSEQLFFKKLGAVIKNTLEKCQRENGFIYFHKVPAEAPALELKASYGLAEPTAFELPPLSAQCTPEVYATFDLTRGHKDDKAKAKPEEEIKPVKEPDLKPQKDTGCVVC

Target ORF information:

RefSeq Version	NM_001007427.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio BRO1 domain and CAAX motif containing (brox), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001007427.2

ORF Insert Sequence:

ATGACTCACT GGTTCCACAG AAACCCTCTG AAAGCCACCG CCCCAGTGTC CTTCAATCTG 
TATGGCGTGG CATCCAGCCC TGCGGCCAAT AAGATCTGCA ATGACCTGCG AACAGCACGC 
GCTCGACTGC TGGACATGTT CACAGATGCC ACATGTTCTC CAGACACCCT GAAGAAATCC 
AGCGATGAGT ATTTTGCTCT TTTACAGGGT TTCATTTTAC CGCTTGATGG CACTACGCAA 
GAGAACAAGC TCAGATTCAT CCAGAATTTC AAATGGACCG ACACTTTACA GGGAAATATA 
GCCAGTGCGC AGCAGGATGC CGTGTTTGAG CTGGTGTCTA TGGCCTTTAA TGTGGCCCTG 
TGGTACACCA AGTTTGCCTC TCGATTGGCT GGAAAGGAGA ACATCACTGA GGAGGAGGCC 
AAAGATGTTC ACAAGAGTTT GAAAATTGCT GCTGGGATAT TTAAAACCCT TAAGGAGACT 
CATATTCCTC GGCTCATCAC TCCAGCAGAG AAGGGTCGAG ATCTGGAGCC GCGAGTTATC 
GACACGTACA TCGTCCAGAG TCAAGCAGAG GCTCAGGAAG TGACCATCGC CAGAGCCATT 
GAGCTGAAGC ACAATGCCTC CCTCATTGCA GCGCTGGCCT TCGAGACTGC CAACTTCTAC 
CAGAAAGCCG ATCACACTCT GAACACTTTG GATCCAGAAT GCAGCAGTAA ATGGAAGAAA 
TACCTGCAGC TGAAGCAGCA CTTCTACATG GCTTACGCTC ACTGCTATCA TGGACAGACT 
CTTCTAGCGG GCGATAAATG TGGAGAGGCC ATAAGATCCC TGCAGGAAGC TGAGAAATGT 
TACTCTCGCG CTGAAGTCTT GTGTAAAGAG TACCGTCAGA TGAAGGGTCC CGGCAGCACC 
GCCAAACCCT CCGAACAGCT CTTCTTCAAG AAACTGGGCG CCGTCATCAA GAACACCTTG 
GAGAAATGCC AGAGAGAGAA CGGCTTCATA TACTTCCACA AGGTTCCGGC TGAAGCTCCG 
GCTCTGGAGT TGAAGGCCAG TTATGGTTTG GCTGAACCCA CTGCGTTCGA GCTCCCGCCG 
CTCAGCGCTC AGTGCACCCC TGAAGTCTAC GCCACCTTTG ACCTGACCAG AGGACACAAA 
GACGACAAGG CGAAAGCGAA GCCTGAAGAG GAGATCAAGC CGGTGAAAGA ACCAGATCTA 
AAGCCGCAGA AGGACACAGG CTGCGTTGTC TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Effects of hypothermia on gene expression in zebrafish gills: upregulation in differentiation and function of ionocytes as compensatory responses.
The Journal of experimental biology211(Pt 19)3077-84(2008 Oct)
Chou MY,Hsiao CD,Chen SC,Chen IW,Liu ST,Hwang PP