MYLKB cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	MYLKB
Entrez Gene ID	492576
Full Name	myosin light chain kinase b
Synonyms	im:7142009,si:dkey-226n16.2
General protein information	Preferred Names myosin light chain kinase b Names myosin light chain kinase, smooth muscle mlck1b unm_sa1486
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009302263.3	XP_009300538.1	myosin light chain kinase, smooth muscle
XM_009302263.2	XP_009300538.1	myosin light chain kinase, smooth muscle

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MYLK	XP_005639631.1
	MYLK	XP_001113525.1
Gallus gallus (chicken)	MYLK	NP_990790.1
Xenopus tropicalis (tropical clawed frog)	LOC100489678	XP_002935598.2
Homo sapiens (human)	MYLK	NP_444253.3
Pan troglodytes (chimpanzee)	MYLK	XP_003950224.1
Mus musculus (house mouse)	Mylk	NP_647461.3
Rattus norvegicus (Norway rat)	Mylk	NP_001099344.2
Danio rerio (zebrafish)	mylkb	XP_002662355.2

Gene Ontology

The following MYLKB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYLKB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa41385	XM_009302263.3 Latest version!	Danio rerio myosin light chain kinase b (mylkb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
View Old Accession Versions >>
ODa41385	XM_009302263.2	Danio rerio myosin light chain kinase b (mylkb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MYLKB ( XM_009302263.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009300538.1 Danio rerio myosin light chain kinase b (mylkb), mRNA.

Price & Availability↑

CloneID	ODa41385
Clone ID Related Accession (Same CDS sequence)	XM_009302263.3 , XM_009302263.2
Accession Version	XM_009302263.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2901bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	myosin light chain kinase, smooth muscle
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009302263.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGACT TTAGGGCAGC TCTAAAACCC AGCTCTGGGC CTAAGCCGGG TTCTGAGATC 
AAAACTAGCA CACCTGCAAA GGTTGACTTT AGATCATTGC TCAATAAAAA AGATGGTTCT 
TCCAAAGCAC CTGTGGCCAC AACACCCCCA AGCCGCGGAC CTTCAGACTT CAGGTCTGTC 
CTGAGCAAGA AACAAGCAGC AGAATCCGTG AAGACGGAGG AAAAAGAACA GAACTCCACA 
AAACCAGCAC CAAAGACACC TCAGAAGGAA AAGCAAAGTG GTGTCAATGG AGTCAATGGT 
GGTGCAGTTG ATAAAAAGAG TACTCTAAAG GAAAACACAA CAACAACAAC AGAGAAAAAA 
GACAGCAAAA CTGATGCAGG GACAATTGGA AAAAAGGAGA AATCCAGCCC GGAGAAGAAG 
ACAAGTGATG GACAGATAAC AGAGTCTAAT GACAAGAAAG TTGTGAATGG AGGAGAGAAG 
GATGCAGGAA AGCCACCGGT TTTCAAACAG CCTCTCAGTG ACATCACTGT GGTGGATGGA 
GAGAGGCTGA GGTTAGAGTG TCAGGTGACC TCTGACCTCC AAGCTGCCAT CACCTGGATA 
CTAGATGGGA AAACGGTGAA GCCATCCAAG TTCATTGTCT TATCACATGA AGGTGAAAAA 
TGCTCGCTTA CAATCGACAA GGCGCTACCT GAAGACGAAG GACGCTACAC ATGTAGAGCA 
GAGAATGCGC ACGGTAAAGC AGAGTGTTCC TGTGTGGTCA CTGTAGATGA TCCTTCAGAC 
CCATCAGCAG ATAAGAAGAA CAGAAAGAGC TCTTCTGCAA CTCCCACTAC AGAAAATGAA 
GCCAGAATAA AGAAGAAACC AGCTCCCACC AAACAAGGTT CTCCACCTCA GTTTCTACAT 
TTCCCTGGAG ATCAGAAGAT CCGGGCGGGA GAAAAGGTGG AGCTGTTGGG CAACTTTGGA 
GGATCGCCAC CAATAACCTG CACTTGGCTT AAATTCAAAA AGCCGATCCA GCCAGGAAAT 
GGAGGCATTT CCATAGAGAC CACAGAGAAC AGCAGTCAGC TGACCATCGC AGCGAGTGAC 
CAGGATCACT GTGGCTGCTA TACTCTTGAA CTGCAGAATA AATTTGGCCT CAAGCAGGCC 
TCGCTGAACC TCACAATCGT AGATAAGCCG GACCCTCCAG CTGGTATCCC CGCTGCCTCT 
GATATCCGCC GGTCCTCGCT TACTCTTTCT TGGTATGGGC CCACTTATGA TGGTGGCAGC 
ATCGTCCAGT CGTACAACCT GGAGATCTGG AACTCGGTGG ACAACACATG GAATGACCTC 
ACATCCTGTA ACAGCACATC TTTCCATGTC CAGAACCTCC TCAGTGACCG TCAGTACAAA 
TTCCGGGTTC GAGCTGTGAA TGTGTATGGG GTTGGAGAGC CCAGTGCAGA ATCTGGACCC 
ATTGAAGTGG GAGCGGAGGA GGTTACAGAG AAAAAGGAAG AGGAGGAGGT TGGAGCCGCT 
GTGTCTGATG ATGATGAAGA GAAAGAGCCA GAATATAGAG ATGTGGTCAT TAAGAAAGAC 
TGCAGCGTCA AGGACTTCTA TGATATAGAG GACCGCTTAG GAACGGGTAA ATTTGGTCAA 
GTCTTCAAAC TAGTGGAGAA GTCCAGTAAA AAGGTATGGG CAGGGAAGTT CATCAAGGCA 
TTCTCACAAA AGGAAAAGGA GAACGTGAGG CAGGAGATCG GCATCATGAA CAGCCTCCAC 
CACCCCAAAC TGGTGCAGTG TATGGACGCC TTCGAGGGAA AATCGGACAT GGTCATGGTC 
ATGGAGATGA TTTCTGGCGG CGAGCTGTTT GAGCGAATCG TGGATGAAGA TTTCGAGCTG 
ACCGAGCGAG AGGTGATTAA GTACATGCTG CAGATCATAG ATGGAGTCCA GTTCATCCAC 
AAGCAAGGCA TCGTCCACTT AGATCTTAAA CCAGAGAACA TCATGTGCAT AAACAAGACC 
GGAAGCAAGA TCAAGCTCAT TGACTTTGGG CTTGCTCGAA GACTAGAGGA TTCTGGCTCT 
CTGAAGGTTT TGTTTGGGAC TCCTGAGTTT GTTGCTCCTG AGGTGATCAA TTATGAGGCT 
ATCAGCTACC CAACAGACAT GTGGAGCATT GGCGTCATCT GTTACATTCT TGTCAGTGGT 
CTATCGCCAT TCATGGGAGA CAACGATAAC GAAACCCTCG CCAACGTCAC CTCAGCCACC 
TGGGATTTTG AGGATGAGGC ATTTGATGAG ATTTCAGATC AAGCCAAAGA CTTCATCAGC 
AGGCTTCTGA AAAAAGACAT GAGGGCCAGA CTGACCTGCG CTCAGTGTTT CGAGCACTCC 
TGGCTCAAAC AGGACACCAA AAACATGGAA GCTAAACAGC TGTCCAAAGA AAGGATGAAA 
AAGTACATCC TGAGACGTCG CTGGCAGAAA ACTGGGAATG CAGTGAGAGC CATTTCCAGA 
TTCAGCTCTA TGGGAATGTT AGGAGGGATC GGCCCAAAGA AAAGCTCTCC AACTGAAGAA 
GAGCCTCCAG CACCATTTCT TGAGAGCGTG GAGGATGAAA ACCACACTCC AGCAGCGCCC 
AGCTTCGCCT CTGTCATTCA GGATGTGGAA GTGGTGGAAG GCAGCGCTGC CCGCTTTGAC 
TGCAAGATTG AAGGCTGCCC AGATCCTGAG GTGGTGTGGT ACAAAGATGA CCAACCTATT 
AAGGAGACAC GCCACTTCCA GATTGACTAT GAGGAGGACG GCCACTGTAG TCTGGTGATC 
TCAGAAGTTT GCCCTGATGA TGACGCCAAG TACACCTGCA AAGCCGTCAA CGGCCTGGGA 
GAAGCATCCT GCACCGCTGA GCTGATGGTG GAGTTTATGC AGGAGGAGGA GGGAGACGCT 
GAAGGAGAGG AAGAGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009300538.1
CDS	298..3198
Translation	MTDFRAALKPSSGPKPGSEIKTSTPAKVDFRSLLNKKDGSSKAPVATTPPSRGPSDFRSVLSKKQAAESVKTEEKEQNSTKPAPKTPQKEKQSGVNGVNGGAVDKKSTLKENTTTTTEKKDSKTDAGTIGKKEKSSPEKKTSDGQITESNDKKVVNGGEKDAGKPPVFKQPLSDITVVDGERLRLECQVTSDLQAAITWILDGKTVKPSKFIVLSHEGEKCSLTIDKALPEDEGRYTCRAENAHGKAECSCVVTVDDPSDPSADKKNRKSSSATPTTENEARIKKKPAPTKQGSPPQFLHFPGDQKIRAGEKVELLGNFGGSPPITCTWLKFKKPIQPGNGGISIETTENSSQLTIAASDQDHCGCYTLELQNKFGLKQASLNLTIVDKPDPPAGIPAASDIRRSSLTLSWYGPTYDGGSIVQSYNLEIWNSVDNTWNDLTSCNSTSFHVQNLLSDRQYKFRVRAVNVYGVGEPSAESGPIEVGAEEVTEKKEEEEVGAAVSDDDEEKEPEYRDVVIKKDCSVKDFYDIEDRLGTGKFGQVFKLVEKSSKKVWAGKFIKAFSQKEKENVRQEIGIMNSLHHPKLVQCMDAFEGKSDMVMVMEMISGGELFERIVDEDFELTEREVIKYMLQIIDGVQFIHKQGIVHLDLKPENIMCINKTGSKIKLIDFGLARRLEDSGSLKVLFGTPEFVAPEVINYEAISYPTDMWSIGVICYILVSGLSPFMGDNDNETLANVTSATWDFEDEAFDEISDQAKDFISRLLKKDMRARLTCAQCFEHSWLKQDTKNMEAKQLSKERMKKYILRRRWQKTGNAVRAISRFSSMGMLGGIGPKKSSPTEEEPPAPFLESVEDENHTPAAPSFASVIQDVEVVEGSAARFDCKIEGCPDPEVVWYKDDQPIKETRHFQIDYEEDGHCSLVISEVCPDDDAKYTCKAVNGLGEASCTAELMVEFMQEEEGDAEGEEED

Target ORF information:

RefSeq Version	XM_009302263.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myosin light chain kinase b (mylkb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302263.3

ORF Insert Sequence:

ATGACAGACT TTAGGGCAGC TCTAAAACCC AGCTCTGGGC CTAAGCCGGG TTCTGAGATC 
AAAACTAGCA CACCTGCAAA GGTTGACTTT AGATCATTGC TCAATAAAAA AGATGGTTCT 
TCCAAAGCAC CTGTGGCCAC AACACCCCCA AGCCGCGGAC CTTCAGACTT CAGGTCTGTC 
CTGAGCAAGA AACAAGCAGC AGAATCCGTG AAGACGGAGG AAAAAGAACA GAACTCCACA 
AAACCAGCAC CAAAGACACC TCAGAAGGAA AAGCAAAGTG GTGTCAATGG AGTCAATGGT 
GGTGCAGTTG ATAAAAAGAG TACTCTAAAG GAAAACACAA CAACAACAAC AGAGAAAAAA 
GACAGCAAAA CTGATGCAGG GACAATTGGA AAAAAGGAGA AATCCAGCCC GGAGAAGAAG 
ACAAGTGATG GACAGATAAC AGAGTCTAAT GACAAGAAAG TTGTGAATGG AGGAGAGAAG 
GATGCAGGAA AGCCACCGGT TTTCAAACAG CCTCTCAGTG ACATCACTGT GGTGGATGGA 
GAGAGGCTGA GGTTAGAGTG TCAGGTGACC TCTGACCTCC AAGCTGCCAT CACCTGGATA 
CTAGATGGGA AAACGGTGAA GCCATCCAAG TTCATTGTCT TATCACATGA AGGTGAAAAA 
TGCTCGCTTA CAATCGACAA GGCGCTACCT GAAGACGAAG GACGCTACAC ATGTAGAGCA 
GAGAATGCGC ACGGTAAAGC AGAGTGTTCC TGTGTGGTCA CTGTAGATGA TCCTTCAGAC 
CCATCAGCAG ATAAGAAGAA CAGAAAGAGC TCTTCTGCAA CTCCCACTAC AGAAAATGAA 
GCCAGAATAA AGAAGAAACC AGCTCCCACC AAACAAGGTT CTCCACCTCA GTTTCTACAT 
TTCCCTGGAG ATCAGAAGAT CCGGGCGGGA GAAAAGGTGG AGCTGTTGGG CAACTTTGGA 
GGATCGCCAC CAATAACCTG CACTTGGCTT AAATTCAAAA AGCCGATCCA GCCAGGAAAT 
GGAGGCATTT CCATAGAGAC CACAGAGAAC AGCAGTCAGC TGACCATCGC AGCGAGTGAC 
CAGGATCACT GTGGCTGCTA TACTCTTGAA CTGCAGAATA AATTTGGCCT CAAGCAGGCC 
TCGCTGAACC TCACAATCGT AGATAAGCCG GACCCTCCAG CTGGTATCCC CGCTGCCTCT 
GATATCCGCC GGTCCTCGCT TACTCTTTCT TGGTATGGGC CCACTTATGA TGGTGGCAGC 
ATCGTCCAGT CGTACAACCT GGAGATCTGG AACTCGGTGG ACAACACATG GAATGACCTC 
ACATCCTGTA ACAGCACATC TTTCCATGTC CAGAACCTCC TCAGTGACCG TCAGTACAAA 
TTCCGGGTTC GAGCTGTGAA TGTGTATGGG GTTGGAGAGC CCAGTGCAGA ATCTGGACCC 
ATTGAAGTGG GAGCGGAGGA GGTTACAGAG AAAAAGGAAG AGGAGGAGGT TGGAGCCGCT 
GTGTCTGATG ATGATGAAGA GAAAGAGCCA GAATATAGAG ATGTGGTCAT TAAGAAAGAC 
TGCAGCGTCA AGGACTTCTA TGATATAGAG GACCGCTTAG GAACGGGTAA ATTTGGTCAA 
GTCTTCAAAC TAGTGGAGAA GTCCAGTAAA AAGGTATGGG CAGGGAAGTT CATCAAGGCA 
TTCTCACAAA AGGAAAAGGA GAACGTGAGG CAGGAGATCG GCATCATGAA CAGCCTCCAC 
CACCCCAAAC TGGTGCAGTG TATGGACGCC TTCGAGGGAA AATCGGACAT GGTCATGGTC 
ATGGAGATGA TTTCTGGCGG CGAGCTGTTT GAGCGAATCG TGGATGAAGA TTTCGAGCTG 
ACCGAGCGAG AGGTGATTAA GTACATGCTG CAGATCATAG ATGGAGTCCA GTTCATCCAC 
AAGCAAGGCA TCGTCCACTT AGATCTTAAA CCAGAGAACA TCATGTGCAT AAACAAGACC 
GGAAGCAAGA TCAAGCTCAT TGACTTTGGG CTTGCTCGAA GACTAGAGGA TTCTGGCTCT 
CTGAAGGTTT TGTTTGGGAC TCCTGAGTTT GTTGCTCCTG AGGTGATCAA TTATGAGGCT 
ATCAGCTACC CAACAGACAT GTGGAGCATT GGCGTCATCT GTTACATTCT TGTCAGTGGT 
CTATCGCCAT TCATGGGAGA CAACGATAAC GAAACCCTCG CCAACGTCAC CTCAGCCACC 
TGGGATTTTG AGGATGAGGC ATTTGATGAG ATTTCAGATC AAGCCAAAGA CTTCATCAGC 
AGGCTTCTGA AAAAAGACAT GAGGGCCAGA CTGACCTGCG CTCAGTGTTT CGAGCACTCC 
TGGCTCAAAC AGGACACCAA AAACATGGAA GCTAAACAGC TGTCCAAAGA AAGGATGAAA 
AAGTACATCC TGAGACGTCG CTGGCAGAAA ACTGGGAATG CAGTGAGAGC CATTTCCAGA 
TTCAGCTCTA TGGGAATGTT AGGAGGGATC GGCCCAAAGA AAAGCTCTCC AACTGAAGAA 
GAGCCTCCAG CACCATTTCT TGAGAGCGTG GAGGATGAAA ACCACACTCC AGCAGCGCCC 
AGCTTCGCCT CTGTCATTCA GGATGTGGAA GTGGTGGAAG GCAGCGCTGC CCGCTTTGAC 
TGCAAGATTG AAGGCTGCCC AGATCCTGAG GTGGTGTGGT ACAAAGATGA CCAACCTATT 
AAGGAGACAC GCCACTTCCA GATTGACTAT GAGGAGGACG GCCACTGTAG TCTGGTGATC 
TCAGAAGTTT GCCCTGATGA TGACGCCAAG TACACCTGCA AAGCCGTCAA CGGCCTGGGA 
GAAGCATCCT GCACCGCTGA GCTGATGGTG GAGTTTATGC AGGAGGAGGA GGGAGACGCT 
GAAGGAGAGG AAGAGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYLKB ( XM_009302263.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009300538.1 Danio rerio myosin light chain kinase b (mylkb), mRNA.

Price & Availability↑

CloneID	ODa41385
Clone ID Related Accession (Same CDS sequence)	XM_009302263.3 , XM_009302263.2
Accession Version	XM_009302263.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2901bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	myosin light chain kinase, smooth muscle
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009302263.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGACT TTAGGGCAGC TCTAAAACCC AGCTCTGGGC CTAAGCCGGG TTCTGAGATC 
AAAACTAGCA CACCTGCAAA GGTTGACTTT AGATCATTGC TCAATAAAAA AGATGGTTCT 
TCCAAAGCAC CTGTGGCCAC AACACCCCCA AGCCGCGGAC CTTCAGACTT CAGGTCTGTC 
CTGAGCAAGA AACAAGCAGC AGAATCCGTG AAGACGGAGG AAAAAGAACA GAACTCCACA 
AAACCAGCAC CAAAGACACC TCAGAAGGAA AAGCAAAGTG GTGTCAATGG AGTCAATGGT 
GGTGCAGTTG ATAAAAAGAG TACTCTAAAG GAAAACACAA CAACAACAAC AGAGAAAAAA 
GACAGCAAAA CTGATGCAGG GACAATTGGA AAAAAGGAGA AATCCAGCCC GGAGAAGAAG 
ACAAGTGATG GACAGATAAC AGAGTCTAAT GACAAGAAAG TTGTGAATGG AGGAGAGAAG 
GATGCAGGAA AGCCACCGGT TTTCAAACAG CCTCTCAGTG ACATCACTGT GGTGGATGGA 
GAGAGGCTGA GGTTAGAGTG TCAGGTGACC TCTGACCTCC AAGCTGCCAT CACCTGGATA 
CTAGATGGGA AAACGGTGAA GCCATCCAAG TTCATTGTCT TATCACATGA AGGTGAAAAA 
TGCTCGCTTA CAATCGACAA GGCGCTACCT GAAGACGAAG GACGCTACAC ATGTAGAGCA 
GAGAATGCGC ACGGTAAAGC AGAGTGTTCC TGTGTGGTCA CTGTAGATGA TCCTTCAGAC 
CCATCAGCAG ATAAGAAGAA CAGAAAGAGC TCTTCTGCAA CTCCCACTAC AGAAAATGAA 
GCCAGAATAA AGAAGAAACC AGCTCCCACC AAACAAGGTT CTCCACCTCA GTTTCTACAT 
TTCCCTGGAG ATCAGAAGAT CCGGGCGGGA GAAAAGGTGG AGCTGTTGGG CAACTTTGGA 
GGATCGCCAC CAATAACCTG CACTTGGCTT AAATTCAAAA AGCCGATCCA GCCAGGAAAT 
GGAGGCATTT CCATAGAGAC CACAGAGAAC AGCAGTCAGC TGACCATCGC AGCGAGTGAC 
CAGGATCACT GTGGCTGCTA TACTCTTGAA CTGCAGAATA AATTTGGCCT CAAGCAGGCC 
TCGCTGAACC TCACAATCGT AGATAAGCCG GACCCTCCAG CTGGTATCCC CGCTGCCTCT 
GATATCCGCC GGTCCTCGCT TACTCTTTCT TGGTATGGGC CCACTTATGA TGGTGGCAGC 
ATCGTCCAGT CGTACAACCT GGAGATCTGG AACTCGGTGG ACAACACATG GAATGACCTC 
ACATCCTGTA ACAGCACATC TTTCCATGTC CAGAACCTCC TCAGTGACCG TCAGTACAAA 
TTCCGGGTTC GAGCTGTGAA TGTGTATGGG GTTGGAGAGC CCAGTGCAGA ATCTGGACCC 
ATTGAAGTGG GAGCGGAGGA GGTTACAGAG AAAAAGGAAG AGGAGGAGGT TGGAGCCGCT 
GTGTCTGATG ATGATGAAGA GAAAGAGCCA GAATATAGAG ATGTGGTCAT TAAGAAAGAC 
TGCAGCGTCA AGGACTTCTA TGATATAGAG GACCGCTTAG GAACGGGTAA ATTTGGTCAA 
GTCTTCAAAC TAGTGGAGAA GTCCAGTAAA AAGGTATGGG CAGGGAAGTT CATCAAGGCA 
TTCTCACAAA AGGAAAAGGA GAACGTGAGG CAGGAGATCG GCATCATGAA CAGCCTCCAC 
CACCCCAAAC TGGTGCAGTG TATGGACGCC TTCGAGGGAA AATCGGACAT GGTCATGGTC 
ATGGAGATGA TTTCTGGCGG CGAGCTGTTT GAGCGAATCG TGGATGAAGA TTTCGAGCTG 
ACCGAGCGAG AGGTGATTAA GTACATGCTG CAGATCATAG ATGGAGTCCA GTTCATCCAC 
AAGCAAGGCA TCGTCCACTT AGATCTTAAA CCAGAGAACA TCATGTGCAT AAACAAGACC 
GGAAGCAAGA TCAAGCTCAT TGACTTTGGG CTTGCTCGAA GACTAGAGGA TTCTGGCTCT 
CTGAAGGTTT TGTTTGGGAC TCCTGAGTTT GTTGCTCCTG AGGTGATCAA TTATGAGGCT 
ATCAGCTACC CAACAGACAT GTGGAGCATT GGCGTCATCT GTTACATTCT TGTCAGTGGT 
CTATCGCCAT TCATGGGAGA CAACGATAAC GAAACCCTCG CCAACGTCAC CTCAGCCACC 
TGGGATTTTG AGGATGAGGC ATTTGATGAG ATTTCAGATC AAGCCAAAGA CTTCATCAGC 
AGGCTTCTGA AAAAAGACAT GAGGGCCAGA CTGACCTGCG CTCAGTGTTT CGAGCACTCC 
TGGCTCAAAC AGGACACCAA AAACATGGAA GCTAAACAGC TGTCCAAAGA AAGGATGAAA 
AAGTACATCC TGAGACGTCG CTGGCAGAAA ACTGGGAATG CAGTGAGAGC CATTTCCAGA 
TTCAGCTCTA TGGGAATGTT AGGAGGGATC GGCCCAAAGA AAAGCTCTCC AACTGAAGAA 
GAGCCTCCAG CACCATTTCT TGAGAGCGTG GAGGATGAAA ACCACACTCC AGCAGCGCCC 
AGCTTCGCCT CTGTCATTCA GGATGTGGAA GTGGTGGAAG GCAGCGCTGC CCGCTTTGAC 
TGCAAGATTG AAGGCTGCCC AGATCCTGAG GTGGTGTGGT ACAAAGATGA CCAACCTATT 
AAGGAGACAC GCCACTTCCA GATTGACTAT GAGGAGGACG GCCACTGTAG TCTGGTGATC 
TCAGAAGTTT GCCCTGATGA TGACGCCAAG TACACCTGCA AAGCCGTCAA CGGCCTGGGA 
GAAGCATCCT GCACCGCTGA GCTGATGGTG GAGTTTATGC AGGAGGAGGA GGGAGACGCT 
GAAGGAGAGG AAGAGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009300538.1
CDS	42..2942
Translation	MTDFRAALKPSSGPKPGSEIKTSTPAKVDFRSLLNKKDGSSKAPVATTPPSRGPSDFRSVLSKKQAAESVKTEEKEQNSTKPAPKTPQKEKQSGVNGVNGGAVDKKSTLKENTTTTTEKKDSKTDAGTIGKKEKSSPEKKTSDGQITESNDKKVVNGGEKDAGKPPVFKQPLSDITVVDGERLRLECQVTSDLQAAITWILDGKTVKPSKFIVLSHEGEKCSLTIDKALPEDEGRYTCRAENAHGKAECSCVVTVDDPSDPSADKKNRKSSSATPTTENEARIKKKPAPTKQGSPPQFLHFPGDQKIRAGEKVELLGNFGGSPPITCTWLKFKKPIQPGNGGISIETTENSSQLTIAASDQDHCGCYTLELQNKFGLKQASLNLTIVDKPDPPAGIPAASDIRRSSLTLSWYGPTYDGGSIVQSYNLEIWNSVDNTWNDLTSCNSTSFHVQNLLSDRQYKFRVRAVNVYGVGEPSAESGPIEVGAEEVTEKKEEEEVGAAVSDDDEEKEPEYRDVVIKKDCSVKDFYDIEDRLGTGKFGQVFKLVEKSSKKVWAGKFIKAFSQKEKENVRQEIGIMNSLHHPKLVQCMDAFEGKSDMVMVMEMISGGELFERIVDEDFELTEREVIKYMLQIIDGVQFIHKQGIVHLDLKPENIMCINKTGSKIKLIDFGLARRLEDSGSLKVLFGTPEFVAPEVINYEAISYPTDMWSIGVICYILVSGLSPFMGDNDNETLANVTSATWDFEDEAFDEISDQAKDFISRLLKKDMRARLTCAQCFEHSWLKQDTKNMEAKQLSKERMKKYILRRRWQKTGNAVRAISRFSSMGMLGGIGPKKSSPTEEEPPAPFLESVEDENHTPAAPSFASVIQDVEVVEGSAARFDCKIEGCPDPEVVWYKDDQPIKETRHFQIDYEEDGHCSLVISEVCPDDDAKYTCKAVNGLGEASCTAELMVEFMQEEEGDAEGEEED

Target ORF information:

RefSeq Version	XM_009302263.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio myosin light chain kinase b (mylkb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302263.2

ORF Insert Sequence:

ATGACAGACT TTAGGGCAGC TCTAAAACCC AGCTCTGGGC CTAAGCCGGG TTCTGAGATC 
AAAACTAGCA CACCTGCAAA GGTTGACTTT AGATCATTGC TCAATAAAAA AGATGGTTCT 
TCCAAAGCAC CTGTGGCCAC AACACCCCCA AGCCGCGGAC CTTCAGACTT CAGGTCTGTC 
CTGAGCAAGA AACAAGCAGC AGAATCCGTG AAGACGGAGG AAAAAGAACA GAACTCCACA 
AAACCAGCAC CAAAGACACC TCAGAAGGAA AAGCAAAGTG GTGTCAATGG AGTCAATGGT 
GGTGCAGTTG ATAAAAAGAG TACTCTAAAG GAAAACACAA CAACAACAAC AGAGAAAAAA 
GACAGCAAAA CTGATGCAGG GACAATTGGA AAAAAGGAGA AATCCAGCCC GGAGAAGAAG 
ACAAGTGATG GACAGATAAC AGAGTCTAAT GACAAGAAAG TTGTGAATGG AGGAGAGAAG 
GATGCAGGAA AGCCACCGGT TTTCAAACAG CCTCTCAGTG ACATCACTGT GGTGGATGGA 
GAGAGGCTGA GGTTAGAGTG TCAGGTGACC TCTGACCTCC AAGCTGCCAT CACCTGGATA 
CTAGATGGGA AAACGGTGAA GCCATCCAAG TTCATTGTCT TATCACATGA AGGTGAAAAA 
TGCTCGCTTA CAATCGACAA GGCGCTACCT GAAGACGAAG GACGCTACAC ATGTAGAGCA 
GAGAATGCGC ACGGTAAAGC AGAGTGTTCC TGTGTGGTCA CTGTAGATGA TCCTTCAGAC 
CCATCAGCAG ATAAGAAGAA CAGAAAGAGC TCTTCTGCAA CTCCCACTAC AGAAAATGAA 
GCCAGAATAA AGAAGAAACC AGCTCCCACC AAACAAGGTT CTCCACCTCA GTTTCTACAT 
TTCCCTGGAG ATCAGAAGAT CCGGGCGGGA GAAAAGGTGG AGCTGTTGGG CAACTTTGGA 
GGATCGCCAC CAATAACCTG CACTTGGCTT AAATTCAAAA AGCCGATCCA GCCAGGAAAT 
GGAGGCATTT CCATAGAGAC CACAGAGAAC AGCAGTCAGC TGACCATCGC AGCGAGTGAC 
CAGGATCACT GTGGCTGCTA TACTCTTGAA CTGCAGAATA AATTTGGCCT CAAGCAGGCC 
TCGCTGAACC TCACAATCGT AGATAAGCCG GACCCTCCAG CTGGTATCCC CGCTGCCTCT 
GATATCCGCC GGTCCTCGCT TACTCTTTCT TGGTATGGGC CCACTTATGA TGGTGGCAGC 
ATCGTCCAGT CGTACAACCT GGAGATCTGG AACTCGGTGG ACAACACATG GAATGACCTC 
ACATCCTGTA ACAGCACATC TTTCCATGTC CAGAACCTCC TCAGTGACCG TCAGTACAAA 
TTCCGGGTTC GAGCTGTGAA TGTGTATGGG GTTGGAGAGC CCAGTGCAGA ATCTGGACCC 
ATTGAAGTGG GAGCGGAGGA GGTTACAGAG AAAAAGGAAG AGGAGGAGGT TGGAGCCGCT 
GTGTCTGATG ATGATGAAGA GAAAGAGCCA GAATATAGAG ATGTGGTCAT TAAGAAAGAC 
TGCAGCGTCA AGGACTTCTA TGATATAGAG GACCGCTTAG GAACGGGTAA ATTTGGTCAA 
GTCTTCAAAC TAGTGGAGAA GTCCAGTAAA AAGGTATGGG CAGGGAAGTT CATCAAGGCA 
TTCTCACAAA AGGAAAAGGA GAACGTGAGG CAGGAGATCG GCATCATGAA CAGCCTCCAC 
CACCCCAAAC TGGTGCAGTG TATGGACGCC TTCGAGGGAA AATCGGACAT GGTCATGGTC 
ATGGAGATGA TTTCTGGCGG CGAGCTGTTT GAGCGAATCG TGGATGAAGA TTTCGAGCTG 
ACCGAGCGAG AGGTGATTAA GTACATGCTG CAGATCATAG ATGGAGTCCA GTTCATCCAC 
AAGCAAGGCA TCGTCCACTT AGATCTTAAA CCAGAGAACA TCATGTGCAT AAACAAGACC 
GGAAGCAAGA TCAAGCTCAT TGACTTTGGG CTTGCTCGAA GACTAGAGGA TTCTGGCTCT 
CTGAAGGTTT TGTTTGGGAC TCCTGAGTTT GTTGCTCCTG AGGTGATCAA TTATGAGGCT 
ATCAGCTACC CAACAGACAT GTGGAGCATT GGCGTCATCT GTTACATTCT TGTCAGTGGT 
CTATCGCCAT TCATGGGAGA CAACGATAAC GAAACCCTCG CCAACGTCAC CTCAGCCACC 
TGGGATTTTG AGGATGAGGC ATTTGATGAG ATTTCAGATC AAGCCAAAGA CTTCATCAGC 
AGGCTTCTGA AAAAAGACAT GAGGGCCAGA CTGACCTGCG CTCAGTGTTT CGAGCACTCC 
TGGCTCAAAC AGGACACCAA AAACATGGAA GCTAAACAGC TGTCCAAAGA AAGGATGAAA 
AAGTACATCC TGAGACGTCG CTGGCAGAAA ACTGGGAATG CAGTGAGAGC CATTTCCAGA 
TTCAGCTCTA TGGGAATGTT AGGAGGGATC GGCCCAAAGA AAAGCTCTCC AACTGAAGAA 
GAGCCTCCAG CACCATTTCT TGAGAGCGTG GAGGATGAAA ACCACACTCC AGCAGCGCCC 
AGCTTCGCCT CTGTCATTCA GGATGTGGAA GTGGTGGAAG GCAGCGCTGC CCGCTTTGAC 
TGCAAGATTG AAGGCTGCCC AGATCCTGAG GTGGTGTGGT ACAAAGATGA CCAACCTATT 
AAGGAGACAC GCCACTTCCA GATTGACTAT GAGGAGGACG GCCACTGTAG TCTGGTGATC 
TCAGAAGTTT GCCCTGATGA TGACGCCAAG TACACCTGCA AAGCCGTCAA CGGCCTGGGA 
GAAGCATCCT GCACCGCTGA GCTGATGGTG GAGTTTATGC AGGAGGAGGA GGGAGACGCT 
GAAGGAGAGG AAGAGGACTG A