prrc1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	prrc1
Entrez Gene ID	474330
Full Name	proline-rich coiled-coil 1
Synonyms	SAG20,fi37a08,id:ibd5136,mys,t2gtl20,wu:fi37a08,zgc:103484
General protein information	Preferred Names proline-rich coiled-coil 1 Names protein PRRC1 misty somites proline-rich and coiled-coil-containing protein 1 protein misty somites tol2 transposon-derived gene trap locus 20
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005155573.4	XP_005155630.1	protein PRRC1 isoform X1
XM_005155573.3	XP_005155630.1	protein PRRC1 isoform X1
NM_001007060.2	NP_001007061.2	protein PRRC1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PRRC1	XP_001159291.1
Xenopus tropicalis (tropical clawed frog)	prrc1	NP_989046.1
Gallus gallus (chicken)	PRRC1	NP_001026588.1
Homo sapiens (human)	PRRC1	NP_570721.1
Mus musculus (house mouse)	Prrc1	NP_082723.2
Danio rerio (zebrafish)	prrc1	NP_001007061.2
	LOC704248	XP_002804577.1
Canis lupus familiaris (dog)	PRRC1	XP_005626538.1
Bos taurus (cattle)	PRRC1	NP_001192929.1
Rattus norvegicus (Norway rat)	Prrc1	NP_001029059.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolutionary divergence of the vertebrate TNFAIP8 gene family: Applying the spotted gar orthology bridge to understand ohnolog loss in teleosts.
Sullivan C, Lage CR, Yoder JA, Postlethwait JH, Kim CH
PloS one12(6)e0179517(2017)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Mys protein regulates protein kinase A activity by interacting with regulatory type Ialpha subunit during vertebrate development.
Kotani T, Iemura S, Natsume T, Kawakami K, Yamashita M
The Journal of biological chemistry285(7)5106-16(2010 Feb)

Misty somites, a maternal effect gene identified by transposon-mediated insertional mutagenesis in zebrafish that is essential for the somite boundary maintenance.
Kotani T, Kawakami K
Developmental biology316(2)383-96(2008 Apr)

A transposon-mediated gene trap approach identifies developmentally regulated genes in zebrafish.
Kawakami K, Takeda H, Kawakami N, Kobayashi M, Matsuda N, Mishina M
Developmental cell7(1)133-44(2004 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results demonstrate that PKA activity regulated by Mys is indispensable for negative regulation of the Hh signaling pathway in Hh-responsive cells.
Title: Mys protein regulates protein kinase A activity by interacting with regulatory type Ialpha subunit during vertebrate development.

The following prrc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the prrc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa15900	NM_001007060.2 Latest version!	Danio rerio proline-rich coiled-coil 1 (prrc1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa15900	XM_005155573.4 Latest version!	Danio rerio proline-rich coiled-coil 1 (prrc1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
View Old Accession Versions >>
ODa15900	XM_005155573.3	Danio rerio proline-rich coiled-coil 1 (prrc1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

prrc1 ( NM_001007060.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001007061.2 Danio rerio proline-rich coiled-coil 1 (prrc1), mRNA.

Price & Availability↑

CloneID	ODa15900
Clone ID Related Accession (Same CDS sequence)	XM_005155573.4 , XM_005155573.3 , NM_001007060.2
Accession Version	NM_001007060.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	protein PRRC1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR396586.8, EH543435.1 and BC083401.1. On Nov 13, 2008 this sequence version replaced NM_001007060.1. ##Evidence-Data-START## Transcript exon combination :: BC083401.1, AB307684.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGGAAG AGAGTGGTAT AGAGAGCACA CCACCCGCTA CCCCTCCACC TCCATCTACA 
GTGGTTGCCA GTCCTCCACC ACTCACCAGC GGTGCCACTC GTCCTCAAAC TGTCCCTAGT 
CCTTCTGGCA TCTCTGCTTT TACCCCAGGA GATTTTTCCA GCCCTGTTCC CTCAGTGGCT 
TTAACTTCCA CGCTGCCACC CATGCAGTCT TCTGTTCCTC CTCTCCCTCT CAGCTCAATA 
TCATCTCCGC TGGGTCTGAG TGGTCCATAT GGCATCCCTA GTGCTTCATT TGCCTCTCCA 
GCTGCCGGTC CTCCACTCAG CACCGGTCCC CCTCTGGGCC CGGTTCTCTC TGCCCCTCCA 
ATGGGCCCTC CCACCACTGG CTTCTCTGTT AGCGGAGGAT ATGACATCAC CCGTGGCCAT 
GCAGGGCGCA CACCTCAGAC GCCATTGATG CCCAGTTTCT CCAGTGCCTC ACCTATGCCA 
GGGATTGTTA CTAATCCCAT GATGCAGCAG CCAGTGTCTG GAGGGTTAGA TGTCAGCTCA 
CCCATTACAT TTCCTGAGGA TGTTGAAGAT CCCAGAGTCA CTCCGGACAG CAACGCACCT 
GGTGGATTAT GGGGCTTCAT CAAGGGTGTT GCTGGAAACC CAATGGTCAA GACCGTCTTG 
GATAAAACCA AACACTCTGT GGAGTCCATG ATCACCACGT TGGATCCTGG GATGGCACCT 
TATATCAAAT CTGGAGGAGA TGTGGACATT GTTGTGACGT CAGATAAGGA AGTGAAGGTG 
GCAGCGGTAC GGGATGCATT TCAGGAGGTG TTTGGTTTGG CCATGGTGAC AGGAGAAGCC 
GGTCAGTCCA ACATTGCCCC ACAGCCAGTG GGATATGCTG CAGGGGTCAA GGGAGCTCAG 
GAGCGCATTG ACAGTCTTCG CCGTGCTGCT GTTATTCATG AGAAGCAGCC TGTGGTCTCT 
GTGGAAAACT TCATCGCTGA ACTCTTTCCA GACAAGTGGT TTGATATAGG TTGTCTGATC 
TTGGATGATC CTGGATATGG GATTCACATT GAGACATTCA CCCAGGCTAC ACCTGTGGCC 
CTTGAGCATG TACAGCAGGC GCAGTCACTG ACTCCTCCAG ATTACAACCT CCGCTGGTCT 
GGTCTTCTAG TGACTGTTGG GGAAGTTCTG GAGAGAAATG TGCCCAATGT CAGCCGCACA 
GATTGGCATC AGGCCTTCAC GGGAATGTCT CGCCGACAGA TGATCTACTC AGCCGCCAAA 
GCTCTGGCTG GCATGTACAA ACTACGTTTG CCCTCCAGGA CAGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001007061.2
CDS	90..1397
Translation	MMEESGIESTPPATPPPPSTVVASPPPLTSGATRPQTVPSPSGISAFTPGDFSSPVPSVALTSTLPPMQSSVPPLPLSSISSPLGLSGPYGIPSASFASPAAGPPLSTGPPLGPVLSAPPMGPPTTGFSVSGGYDITRGHAGRTPQTPLMPSFSSASPMPGIVTNPMMQQPVSGGLDVSSPITFPEDVEDPRVTPDSNAPGGLWGFIKGVAGNPMVKTVLDKTKHSVESMITTLDPGMAPYIKSGGDVDIVVTSDKEVKVAAVRDAFQEVFGLAMVTGEAGQSNIAPQPVGYAAGVKGAQERIDSLRRAAVIHEKQPVVSVENFIAELFPDKWFDIGCLILDDPGYGIHIETFTQATPVALEHVQQAQSLTPPDYNLRWSGLLVTVGEVLERNVPNVSRTDWHQAFTGMSRRQMIYSAAKALAGMYKLRLPSRTV

Target ORF information:

RefSeq Version	NM_001007060.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio proline-rich coiled-coil 1 (prrc1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001007060.2

ORF Insert Sequence:

ATGATGGAAG AGAGTGGTAT AGAGAGCACA CCACCCGCTA CCCCTCCACC TCCATCTACA 
GTGGTTGCCA GTCCTCCACC ACTCACCAGC GGTGCCACTC GTCCTCAAAC TGTCCCTAGT 
CCTTCTGGCA TCTCTGCTTT TACCCCAGGA GATTTTTCCA GCCCTGTTCC CTCAGTGGCT 
TTAACTTCCA CGCTGCCACC CATGCAGTCT TCTGTTCCTC CTCTCCCTCT CAGCTCAATA 
TCATCTCCGC TGGGTCTGAG TGGTCCATAT GGCATCCCTA GTGCTTCATT TGCCTCTCCA 
GCTGCCGGTC CTCCACTCAG CACCGGTCCC CCTCTGGGCC CGGTTCTCTC TGCCCCTCCA 
ATGGGCCCTC CCACCACTGG CTTCTCTGTT AGCGGAGGAT ATGACATCAC CCGTGGCCAT 
GCAGGGCGCA CACCTCAGAC GCCATTGATG CCCAGTTTCT CCAGTGCCTC ACCTATGCCA 
GGGATTGTTA CTAATCCCAT GATGCAGCAG CCAGTGTCTG GAGGGTTAGA TGTCAGCTCA 
CCCATTACAT TTCCTGAGGA TGTTGAAGAT CCCAGAGTCA CTCCGGACAG CAACGCACCT 
GGTGGATTAT GGGGCTTCAT CAAGGGTGTT GCTGGAAACC CAATGGTCAA GACCGTCTTG 
GATAAAACCA AACACTCTGT GGAGTCCATG ATCACCACGT TGGATCCTGG GATGGCACCT 
TATATCAAAT CTGGAGGAGA TGTGGACATT GTTGTGACGT CAGATAAGGA AGTGAAGGTG 
GCAGCGGTAC GGGATGCATT TCAGGAGGTG TTTGGTTTGG CCATGGTGAC AGGAGAAGCC 
GGTCAGTCCA ACATTGCCCC ACAGCCAGTG GGATATGCTG CAGGGGTCAA GGGAGCTCAG 
GAGCGCATTG ACAGTCTTCG CCGTGCTGCT GTTATTCATG AGAAGCAGCC TGTGGTCTCT 
GTGGAAAACT TCATCGCTGA ACTCTTTCCA GACAAGTGGT TTGATATAGG TTGTCTGATC 
TTGGATGATC CTGGATATGG GATTCACATT GAGACATTCA CCCAGGCTAC ACCTGTGGCC 
CTTGAGCATG TACAGCAGGC GCAGTCACTG ACTCCTCCAG ATTACAACCT CCGCTGGTCT 
GGTCTTCTAG TGACTGTTGG GGAAGTTCTG GAGAGAAATG TGCCCAATGT CAGCCGCACA 
GATTGGCATC AGGCCTTCAC GGGAATGTCT CGCCGACAGA TGATCTACTC AGCCGCCAAA 
GCTCTGGCTG GCATGTACAA ACTACGTTTG CCCTCCAGGA CAGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prrc1 ( XM_005155573.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155630.1 Danio rerio proline-rich coiled-coil 1 (prrc1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa15900
Clone ID Related Accession (Same CDS sequence)	XM_005155573.4 , XM_005155573.3 , NM_001007060.2
Accession Version	XM_005155573.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein PRRC1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005155573.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAG AGAGTGGTAT AGAGAGCACA CCACCCGCTA CCCCTCCACC TCCATCTACA 
GTGGTTGCCA GTCCTCCACC ACTCACCAGC GGTGCCACTC GTCCTCAAAC TGTCCCTAGT 
CCTTCTGGCA TCTCTGCTTT TACCCCAGGA GATTTTTCCA GCCCTGTTCC CTCAGTGGCT 
TTAACTTCCA CGCTGCCACC CATGCAGTCT TCTGTTCCTC CTCTCCCTCT CAGCTCAATA 
TCATCTCCGC TGGGTCTGAG TGGTCCATAT GGCATCCCTA GTGCTTCATT TGCCTCTCCA 
GCTGCCGGTC CTCCACTCAG CACCGGTCCC CCTCTGGGCC CGGTTCTCTC TGCCCCTCCA 
ATGGGCCCTC CCACCACTGG CTTCTCTGTT AGCGGAGGAT ATGACATCAC CCGTGGCCAT 
GCAGGGCGCA CACCTCAGAC GCCATTGATG CCCAGTTTCT CCAGTGCCTC ACCTATGCCA 
GGGATTGTTA CTAATCCCAT GATGCAGCAG CCAGTGTCTG GAGGGTTAGA TGTCAGCTCA 
CCCATTACAT TTCCTGAGGA TGTTGAAGAT CCCAGAGTCA CTCCGGACAG CAACGCACCT 
GGTGGATTAT GGGGCTTCAT CAAGGGTGTT GCTGGAAACC CAATGGTCAA GACCGTCTTG 
GATAAAACCA AACACTCTGT GGAGTCCATG ATCACCACGT TGGATCCTGG GATGGCACCT 
TATATCAAAT CTGGAGGAGA TGTGGACATT GTTGTGACGT CAGATAAGGA AGTGAAGGTG 
GCAGCGGTAC GGGATGCATT TCAGGAGGTG TTTGGTTTGG CCATGGTGAC AGGAGAAGCC 
GGTCAGTCCA ACATTGCCCC ACAGCCAGTG GGATATGCTG CAGGGGTCAA GGGAGCTCAG 
GAGCGCATTG ACAGTCTTCG CCGTGCTGCT GTTATTCATG AGAAGCAGCC TGTGGTCTCT 
GTGGAAAACT TCATCGCTGA ACTCTTTCCA GACAAGTGGT TTGATATAGG TTGTCTGATC 
TTGGATGATC CTGGATATGG GATTCACATT GAGACATTCA CCCAGGCTAC ACCTGTGGCC 
CTTGAGCATG TACAGCAGGC GCAGTCACTG ACTCCTCCAG ATTACAACCT CCGCTGGTCT 
GGTCTTCTAG TGACTGTTGG GGAAGTTCTG GAGAGAAATG TGCCCAATGT CAGCCGCACA 
GATTGGCATC AGGCCTTCAC GGGAATGTCT CGCCGACAGA TGATCTACTC AGCCGCCAAA 
GCTCTGGCTG GCATGTACAA ACTACGTTTG CCCTCCAGGA CAGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155630.1
CDS	139..1446
Translation	MMEESGIESTPPATPPPPSTVVASPPPLTSGATRPQTVPSPSGISAFTPGDFSSPVPSVALTSTLPPMQSSVPPLPLSSISSPLGLSGPYGIPSASFASPAAGPPLSTGPPLGPVLSAPPMGPPTTGFSVSGGYDITRGHAGRTPQTPLMPSFSSASPMPGIVTNPMMQQPVSGGLDVSSPITFPEDVEDPRVTPDSNAPGGLWGFIKGVAGNPMVKTVLDKTKHSVESMITTLDPGMAPYIKSGGDVDIVVTSDKEVKVAAVRDAFQEVFGLAMVTGEAGQSNIAPQPVGYAAGVKGAQERIDSLRRAAVIHEKQPVVSVENFIAELFPDKWFDIGCLILDDPGYGIHIETFTQATPVALEHVQQAQSLTPPDYNLRWSGLLVTVGEVLERNVPNVSRTDWHQAFTGMSRRQMIYSAAKALAGMYKLRLPSRTV

Target ORF information:

RefSeq Version	XM_005155573.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio proline-rich coiled-coil 1 (prrc1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155573.4

ORF Insert Sequence:

ATGATGGAAG AGAGTGGTAT AGAGAGCACA CCACCCGCTA CCCCTCCACC TCCATCTACA 
GTGGTTGCCA GTCCTCCACC ACTCACCAGC GGTGCCACTC GTCCTCAAAC TGTCCCTAGT 
CCTTCTGGCA TCTCTGCTTT TACCCCAGGA GATTTTTCCA GCCCTGTTCC CTCAGTGGCT 
TTAACTTCCA CGCTGCCACC CATGCAGTCT TCTGTTCCTC CTCTCCCTCT CAGCTCAATA 
TCATCTCCGC TGGGTCTGAG TGGTCCATAT GGCATCCCTA GTGCTTCATT TGCCTCTCCA 
GCTGCCGGTC CTCCACTCAG CACCGGTCCC CCTCTGGGCC CGGTTCTCTC TGCCCCTCCA 
ATGGGCCCTC CCACCACTGG CTTCTCTGTT AGCGGAGGAT ATGACATCAC CCGTGGCCAT 
GCAGGGCGCA CACCTCAGAC GCCATTGATG CCCAGTTTCT CCAGTGCCTC ACCTATGCCA 
GGGATTGTTA CTAATCCCAT GATGCAGCAG CCAGTGTCTG GAGGGTTAGA TGTCAGCTCA 
CCCATTACAT TTCCTGAGGA TGTTGAAGAT CCCAGAGTCA CTCCGGACAG CAACGCACCT 
GGTGGATTAT GGGGCTTCAT CAAGGGTGTT GCTGGAAACC CAATGGTCAA GACCGTCTTG 
GATAAAACCA AACACTCTGT GGAGTCCATG ATCACCACGT TGGATCCTGG GATGGCACCT 
TATATCAAAT CTGGAGGAGA TGTGGACATT GTTGTGACGT CAGATAAGGA AGTGAAGGTG 
GCAGCGGTAC GGGATGCATT TCAGGAGGTG TTTGGTTTGG CCATGGTGAC AGGAGAAGCC 
GGTCAGTCCA ACATTGCCCC ACAGCCAGTG GGATATGCTG CAGGGGTCAA GGGAGCTCAG 
GAGCGCATTG ACAGTCTTCG CCGTGCTGCT GTTATTCATG AGAAGCAGCC TGTGGTCTCT 
GTGGAAAACT TCATCGCTGA ACTCTTTCCA GACAAGTGGT TTGATATAGG TTGTCTGATC 
TTGGATGATC CTGGATATGG GATTCACATT GAGACATTCA CCCAGGCTAC ACCTGTGGCC 
CTTGAGCATG TACAGCAGGC GCAGTCACTG ACTCCTCCAG ATTACAACCT CCGCTGGTCT 
GGTCTTCTAG TGACTGTTGG GGAAGTTCTG GAGAGAAATG TGCCCAATGT CAGCCGCACA 
GATTGGCATC AGGCCTTCAC GGGAATGTCT CGCCGACAGA TGATCTACTC AGCCGCCAAA 
GCTCTGGCTG GCATGTACAA ACTACGTTTG CCCTCCAGGA CAGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prrc1 ( XM_005155573.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155630.1 Danio rerio proline-rich coiled-coil 1 (prrc1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa15900
Clone ID Related Accession (Same CDS sequence)	XM_005155573.4 , XM_005155573.3 , NM_001007060.2
Accession Version	XM_005155573.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein PRRC1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005155573.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAG AGAGTGGTAT AGAGAGCACA CCACCCGCTA CCCCTCCACC TCCATCTACA 
GTGGTTGCCA GTCCTCCACC ACTCACCAGC GGTGCCACTC GTCCTCAAAC TGTCCCTAGT 
CCTTCTGGCA TCTCTGCTTT TACCCCAGGA GATTTTTCCA GCCCTGTTCC CTCAGTGGCT 
TTAACTTCCA CGCTGCCACC CATGCAGTCT TCTGTTCCTC CTCTCCCTCT CAGCTCAATA 
TCATCTCCGC TGGGTCTGAG TGGTCCATAT GGCATCCCTA GTGCTTCATT TGCCTCTCCA 
GCTGCCGGTC CTCCACTCAG CACCGGTCCC CCTCTGGGCC CGGTTCTCTC TGCCCCTCCA 
ATGGGCCCTC CCACCACTGG CTTCTCTGTT AGCGGAGGAT ATGACATCAC CCGTGGCCAT 
GCAGGGCGCA CACCTCAGAC GCCATTGATG CCCAGTTTCT CCAGTGCCTC ACCTATGCCA 
GGGATTGTTA CTAATCCCAT GATGCAGCAG CCAGTGTCTG GAGGGTTAGA TGTCAGCTCA 
CCCATTACAT TTCCTGAGGA TGTTGAAGAT CCCAGAGTCA CTCCGGACAG CAACGCACCT 
GGTGGATTAT GGGGCTTCAT CAAGGGTGTT GCTGGAAACC CAATGGTCAA GACCGTCTTG 
GATAAAACCA AACACTCTGT GGAGTCCATG ATCACCACGT TGGATCCTGG GATGGCACCT 
TATATCAAAT CTGGAGGAGA TGTGGACATT GTTGTGACGT CAGATAAGGA AGTGAAGGTG 
GCAGCGGTAC GGGATGCATT TCAGGAGGTG TTTGGTTTGG CCATGGTGAC AGGAGAAGCC 
GGTCAGTCCA ACATTGCCCC ACAGCCAGTG GGATATGCTG CAGGGGTCAA GGGAGCTCAG 
GAGCGCATTG ACAGTCTTCG CCGTGCTGCT GTTATTCATG AGAAGCAGCC TGTGGTCTCT 
GTGGAAAACT TCATCGCTGA ACTCTTTCCA GACAAGTGGT TTGATATAGG TTGTCTGATC 
TTGGATGATC CTGGATATGG GATTCACATT GAGACATTCA CCCAGGCTAC ACCTGTGGCC 
CTTGAGCATG TACAGCAGGC GCAGTCACTG ACTCCTCCAG ATTACAACCT CCGCTGGTCT 
GGTCTTCTAG TGACTGTTGG GGAAGTTCTG GAGAGAAATG TGCCCAATGT CAGCCGCACA 
GATTGGCATC AGGCCTTCAC GGGAATGTCT CGCCGACAGA TGATCTACTC AGCCGCCAAA 
GCTCTGGCTG GCATGTACAA ACTACGTTTG CCCTCCAGGA CAGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155630.1
CDS	118..1425
Translation	MMEESGIESTPPATPPPPSTVVASPPPLTSGATRPQTVPSPSGISAFTPGDFSSPVPSVALTSTLPPMQSSVPPLPLSSISSPLGLSGPYGIPSASFASPAAGPPLSTGPPLGPVLSAPPMGPPTTGFSVSGGYDITRGHAGRTPQTPLMPSFSSASPMPGIVTNPMMQQPVSGGLDVSSPITFPEDVEDPRVTPDSNAPGGLWGFIKGVAGNPMVKTVLDKTKHSVESMITTLDPGMAPYIKSGGDVDIVVTSDKEVKVAAVRDAFQEVFGLAMVTGEAGQSNIAPQPVGYAAGVKGAQERIDSLRRAAVIHEKQPVVSVENFIAELFPDKWFDIGCLILDDPGYGIHIETFTQATPVALEHVQQAQSLTPPDYNLRWSGLLVTVGEVLERNVPNVSRTDWHQAFTGMSRRQMIYSAAKALAGMYKLRLPSRTV

Target ORF information:

RefSeq Version	XM_005155573.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio proline-rich coiled-coil 1 (prrc1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155573.3

ORF Insert Sequence:

ATGATGGAAG AGAGTGGTAT AGAGAGCACA CCACCCGCTA CCCCTCCACC TCCATCTACA 
GTGGTTGCCA GTCCTCCACC ACTCACCAGC GGTGCCACTC GTCCTCAAAC TGTCCCTAGT 
CCTTCTGGCA TCTCTGCTTT TACCCCAGGA GATTTTTCCA GCCCTGTTCC CTCAGTGGCT 
TTAACTTCCA CGCTGCCACC CATGCAGTCT TCTGTTCCTC CTCTCCCTCT CAGCTCAATA 
TCATCTCCGC TGGGTCTGAG TGGTCCATAT GGCATCCCTA GTGCTTCATT TGCCTCTCCA 
GCTGCCGGTC CTCCACTCAG CACCGGTCCC CCTCTGGGCC CGGTTCTCTC TGCCCCTCCA 
ATGGGCCCTC CCACCACTGG CTTCTCTGTT AGCGGAGGAT ATGACATCAC CCGTGGCCAT 
GCAGGGCGCA CACCTCAGAC GCCATTGATG CCCAGTTTCT CCAGTGCCTC ACCTATGCCA 
GGGATTGTTA CTAATCCCAT GATGCAGCAG CCAGTGTCTG GAGGGTTAGA TGTCAGCTCA 
CCCATTACAT TTCCTGAGGA TGTTGAAGAT CCCAGAGTCA CTCCGGACAG CAACGCACCT 
GGTGGATTAT GGGGCTTCAT CAAGGGTGTT GCTGGAAACC CAATGGTCAA GACCGTCTTG 
GATAAAACCA AACACTCTGT GGAGTCCATG ATCACCACGT TGGATCCTGG GATGGCACCT 
TATATCAAAT CTGGAGGAGA TGTGGACATT GTTGTGACGT CAGATAAGGA AGTGAAGGTG 
GCAGCGGTAC GGGATGCATT TCAGGAGGTG TTTGGTTTGG CCATGGTGAC AGGAGAAGCC 
GGTCAGTCCA ACATTGCCCC ACAGCCAGTG GGATATGCTG CAGGGGTCAA GGGAGCTCAG 
GAGCGCATTG ACAGTCTTCG CCGTGCTGCT GTTATTCATG AGAAGCAGCC TGTGGTCTCT 
GTGGAAAACT TCATCGCTGA ACTCTTTCCA GACAAGTGGT TTGATATAGG TTGTCTGATC 
TTGGATGATC CTGGATATGG GATTCACATT GAGACATTCA CCCAGGCTAC ACCTGTGGCC 
CTTGAGCATG TACAGCAGGC GCAGTCACTG ACTCCTCCAG ATTACAACCT CCGCTGGTCT 
GGTCTTCTAG TGACTGTTGG GGAAGTTCTG GAGAGAAATG TGCCCAATGT CAGCCGCACA 
GATTGGCATC AGGCCTTCAC GGGAATGTCT CGCCGACAGA TGATCTACTC AGCCGCCAAA 
GCTCTGGCTG GCATGTACAA ACTACGTTTG CCCTCCAGGA CAGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolutionary divergence of the vertebrate TNFAIP8 gene family: Applying the spotted gar orthology bridge to understand ohnolog loss in teleosts.
PloS one12(6)e0179517(2017)
Sullivan C,Lage CR,Yoder JA,Postlethwait JH,Kim CH

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Mys protein regulates protein kinase A activity by interacting with regulatory type Ialpha subunit during vertebrate development.
The Journal of biological chemistry285(7)5106-16(2010 Feb)
Kotani T,Iemura S,Natsume T,Kawakami K,Yamashita M

Misty somites, a maternal effect gene identified by transposon-mediated insertional mutagenesis in zebrafish that is essential for the somite boundary maintenance.
Developmental biology316(2)383-96(2008 Apr)
Kotani T,Kawakami K

A transposon-mediated gene trap approach identifies developmentally regulated genes in zebrafish.
Developmental cell7(1)133-44(2004 Jul)
Kawakami K,Takeda H,Kawakami N,Kobayashi M,Matsuda N,Mishina M

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prrc1 cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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