snrnp70 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	snrnp70
Entrez Gene ID	445398
Full Name	small nuclear ribonucleoprotein 70 (U1)
Synonyms	snrp70,wu:fc20b04,zgc:136450
General protein information	Preferred Names small nuclear ribonucleoprotein 70 (U1) Names U1 small nuclear ribonucleoprotein 70 kDa U1 small nuclear ribonucleoprotein polypeptide A U1 small ribonucleoprotein 70kd small nuclear ribonucleoprotein 70kDa
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001003875.1	NP_001003875.1	U1 small nuclear ribonucleoprotein 70 kDa
NM_001003875.2	NP_001003875.1	U1 small nuclear ribonucleoprotein 70 kDa
XM_017354005.1	XP_017209494.1	U1 small nuclear ribonucleoprotein 70 kDa isoform X1
XM_017354005.2	XP_017209494.1	U1 small nuclear ribonucleoprotein 70 kDa isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Non-nuclear Pool of Splicing Factor SFPQ Regulates Axonal Transcripts Required for Normal Motor Development.
Thomas-Jinu S, Gordon PM, Fielding T, Taylor R, Smith BN, Snowden V, Blanc E, Vance C, Topp S, Wong CH, Bielen H, Williams KL, McCann EP, Nicholson GA, Pan-Vazquez A, Fox AH, Bond CS, Talbot WS, Blair IP, Shaw CE, Houart C
Neuron94(2)322-336.e5(2017 Apr)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

U1 snRNP is mislocalized in ALS patient fibroblasts bearing NLS mutations in FUS and is required for motor neuron outgrowth in zebrafish.
Yu Y, Chi B, Xia W, Gangopadhyay J, Yamazaki T, Winkelbauer-Hurt ME, Yin S, Eliasse Y, Adams E, Shaw CE, Reed R
Nucleic acids research43(6)3208-18(2015 Mar)

RNA-Seq analysis in mutant zebrafish reveals role of U1C protein in alternative splicing regulation.
R?sel TD, Hung LH, Medenbach J, Donde K, Starke S, Benes V, R?tsch G, Bindereif A
The EMBO journal30(10)1965-76(2011 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings provide evidence for a new role of a general small nuclear ribonucleoprotein particle protein, U1C, as a mediator of alternative splicing regulation.
Title: RNA-Seq analysis in mutant zebrafish reveals role of U1C protein in alternative splicing regulation.

The following snrnp70 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the snrnp70 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa34065	NM_001003875.1 Latest version!	Danio rerio small nuclear ribonucleoprotein 70 (U1) (snrnp70), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa159839	NM_001003875.2 Latest version!	Danio rerio small nuclear ribonucleoprotein 70 (U1) (snrnp70), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ODa58036	XM_017354005.2 Latest version!	Danio rerio small nuclear ribonucleoprotein 70 (U1) (snrnp70), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
View Old Accession Versions >>
ODa58036	XM_017354005.1	Danio rerio small nuclear ribonucleoprotein 70 (U1) (snrnp70), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

snrnp70 ( NM_001003875.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001003875.1 Danio rerio small nuclear ribonucleoprotein 70 (U1) (snrnp70), mRNA.

Price & Availability↑

CloneID	ODa34065
Clone ID Related Accession (Same CDS sequence)	NM_001003875.1
Accession Version	NM_001003875.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1488bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-05
Organism	Danio rerio(zebrafish)
Product	U1 small nuclear ribonucleoprotein 70 kDa
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY648811.1. ##Evidence-Data-START## Transcript exon combination :: AY648811.1, BC077089.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGACTCAGT TTCTTCCGCC GAACCTGTTG GCATTGTTTG CCCCGCGGGA CCCCATACCG 
TTCCTGCCTC AACTGGGAAA GCTTCCTCAC GAGAAACACC ACAACCAGCC CTACTGCGGA 
ATTGCTCCTT TTATCCGGCA TTTTGAGGAC CCCAGAGATG CCCCTCCACC CACAAGAGCA 
GAGACACGGG AGGAGAGACT GGAGAGGAAG AGAAGAGAAA AAATGGAGAG GAGGCAAGTT 
GTGGTTGAAG GAGAGCTGAA ATTATGGGAC CCACATAATG ATGCTAATGC CCAAGGAGAT 
GCGTTTAAGA CCCTGTTTGT GGCTCGAATT AATTATGATA CCACAGAATC CAAACTGCGG 
CGAGAGTTTG AGGTCTATGG TCCTATCAAA CGGATCTACA TTGTGTACAA CAAGAAAACT 
GGAAAACCAA GAGGCTATGC GTTCATTGAA TACGAGCACG AGAGAGACAT GCACTCTGCT 
TATAAGCACG CGGATGGCAA AAAGATTGAT GGTCGTAGAG TGCTTGTTGA TGTTGAAAGA 
GGACGAACGG TAAAAGGATG GCGCCCCCGG CGACTTGGTG GGGGTCTTGG TGGCACAAGG 
CGAGGAGGCG CAGATGTCAA CATCAAGCAC TCTGGCAGAG ACGACACATC TCGTTATGAT 
GACCGACCAA TTGGAAGTGA TCGCGATCGG GATCGTGGTG AACGCCGTGA ACGCAGTCGG 
GAACGTGATC GAGACAAGGA ACGTGGAGAA CGCAGACGTT CTCGGTCCAG AGAGAGACGC 
AGGCACACTA GGTCTCATGA GCGTGAGAGG GCCGCTGCAG GGGATGAGGC TGGGGGCAGC 
AGCCGACGAC GAGAGAGGGA AAGAGAACGA GGTGGTGCTG GAGCTGGAGA AAGCAGAGAG 
AGGAGTAGAG ACCGGGATCG AGATAGAGAC CGCAAGAGAA GGAGCAGGAG CAGGGATAGG 
AAGAGAGACA GGGAAAGAGG AAAAGGTGCA GAAGGAGGGG AGGAAGGCAT GGGCGGGCTA 
GTTGATGGCA TGATGCCTGA AGGGGGAGAT AGGGGCATAG AGGACCCCTT GGGTGGTGAA 
CCAGGGCGAG GTGAGGAGGG AGGACCTGAA GGAGAGGAAA GGGGGAGGGA TCGAGACAAA 
GACAGGGAAA GAGATCGTGA TAGGAAGCGC AGCCATCGAG ATAAAGATAG AGAAAGGGAT 
CGGGACAGAA GGCGGGACAG GGATAGAGAT CGTGACCATA AGCGAGACCG TGGAGATCGG 
GACCGTGGGG AGCGAAGGGA AGATCGACAC ATTACCTCCT CCGGAGATCA TGAGGGTTTG 
GGTAATGGAG GTGAAGAAGG TGAAGAGCCA GTGCCACCGC AATCAGAAGA AGGTTCACAG 
GATGGGATGA GGATGATGAT GATGGATCAG GATTCCATGC AGTCGGGAGA GGGCTATGCC 
TCCAATGAAA ATGGGTACAG GATGGAGGCC CAGGGTGATG AGTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003875.1
CDS	152..1639
Translation	MTQFLPPNLLALFAPRDPIPFLPQLGKLPHEKHHNQPYCGIAPFIRHFEDPRDAPPPTRAETREERLERKRREKMERRQVVVEGELKLWDPHNDANAQGDAFKTLFVARINYDTTESKLRREFEVYGPIKRIYIVYNKKTGKPRGYAFIEYEHERDMHSAYKHADGKKIDGRRVLVDVERGRTVKGWRPRRLGGGLGGTRRGGADVNIKHSGRDDTSRYDDRPIGSDRDRDRGERRERSRERDRDKERGERRRSRSRERRRHTRSHERERAAAGDEAGGSSRRRERERERGGAGAGESRERSRDRDRDRDRKRRSRSRDRKRDRERGKGAEGGEEGMGGLVDGMMPEGGDRGIEDPLGGEPGRGEEGGPEGEERGRDRDKDRERDRDRKRSHRDKDRERDRDRRRDRDRDRDHKRDRGDRDRGERREDRHITSSGDHEGLGNGGEEGEEPVPPQSEEGSQDGMRMMMMDQDSMQSGEGYASNENGYRMEAQGDEY

Target ORF information:

RefSeq Version	NM_001003875.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio small nuclear ribonucleoprotein 70 (U1) (snrnp70), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003875.1

ORF Insert Sequence:

ATGACTCAGT TTCTTCCGCC GAACCTGTTG GCATTGTTTG CCCCGCGGGA CCCCATACCG 
TTCCTGCCTC AACTGGGAAA GCTTCCTCAC GAGAAACACC ACAACCAGCC CTACTGCGGA 
ATTGCTCCTT TTATCCGGCA TTTTGAGGAC CCCAGAGATG CCCCTCCACC CACAAGAGCA 
GAGACACGGG AGGAGAGACT GGAGAGGAAG AGAAGAGAAA AAATGGAGAG GAGGCAAGTT 
GTGGTTGAAG GAGAGCTGAA ATTATGGGAC CCACATAATG ATGCTAATGC CCAAGGAGAT 
GCGTTTAAGA CCCTGTTTGT GGCTCGAATT AATTATGATA CCACAGAATC CAAACTGCGG 
CGAGAGTTTG AGGTCTATGG TCCTATCAAA CGGATCTACA TTGTGTACAA CAAGAAAACT 
GGAAAACCAA GAGGCTATGC GTTCATTGAA TACGAGCACG AGAGAGACAT GCACTCTGCT 
TATAAGCACG CGGATGGCAA AAAGATTGAT GGTCGTAGAG TGCTTGTTGA TGTTGAAAGA 
GGACGAACGG TAAAAGGATG GCGCCCCCGG CGACTTGGTG GGGGTCTTGG TGGCACAAGG 
CGAGGAGGCG CAGATGTCAA CATCAAGCAC TCTGGCAGAG ACGACACATC TCGTTATGAT 
GACCGACCAA TTGGAAGTGA TCGCGATCGG GATCGTGGTG AACGCCGTGA ACGCAGTCGG 
GAACGTGATC GAGACAAGGA ACGTGGAGAA CGCAGACGTT CTCGGTCCAG AGAGAGACGC 
AGGCACACTA GGTCTCATGA GCGTGAGAGG GCCGCTGCAG GGGATGAGGC TGGGGGCAGC 
AGCCGACGAC GAGAGAGGGA AAGAGAACGA GGTGGTGCTG GAGCTGGAGA AAGCAGAGAG 
AGGAGTAGAG ACCGGGATCG AGATAGAGAC CGCAAGAGAA GGAGCAGGAG CAGGGATAGG 
AAGAGAGACA GGGAAAGAGG AAAAGGTGCA GAAGGAGGGG AGGAAGGCAT GGGCGGGCTA 
GTTGATGGCA TGATGCCTGA AGGGGGAGAT AGGGGCATAG AGGACCCCTT GGGTGGTGAA 
CCAGGGCGAG GTGAGGAGGG AGGACCTGAA GGAGAGGAAA GGGGGAGGGA TCGAGACAAA 
GACAGGGAAA GAGATCGTGA TAGGAAGCGC AGCCATCGAG ATAAAGATAG AGAAAGGGAT 
CGGGACAGAA GGCGGGACAG GGATAGAGAT CGTGACCATA AGCGAGACCG TGGAGATCGG 
GACCGTGGGG AGCGAAGGGA AGATCGACAC ATTACCTCCT CCGGAGATCA TGAGGGTTTG 
GGTAATGGAG GTGAAGAAGG TGAAGAGCCA GTGCCACCGC AATCAGAAGA AGGTTCACAG 
GATGGGATGA GGATGATGAT GATGGATCAG GATTCCATGC AGTCGGGAGA GGGCTATGCC 
TCCAATGAAA ATGGGTACAG GATGGAGGCC CAGGGTGATG AGTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

snrnp70 ( NM_001003875.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001003875.1 Danio rerio small nuclear ribonucleoprotein 70 (U1) (snrnp70), mRNA.

Price & Availability↑

CloneID	ODa159839
Clone ID Related Accession (Same CDS sequence)	NM_001003875.2
Accession Version	NM_001003875.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1488bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-21
Organism	Danio rerio(zebrafish)
Product	U1 small nuclear ribonucleoprotein 70 kDa
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX324157.4. On Aug 24, 2018 this sequence version replaced NM_001003875.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC117602.1, AY648811.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGACTCAGT TTCTTCCGCC GAACCTGTTG GCATTGTTTG CCCCGCGGGA CCCCATACCG 
TTCCTGCCTC AACTGGGAAA GCTTCCTCAC GAGAAACACC ACAACCAGCC CTACTGCGGA 
ATTGCTCCTT TTATCCGGCA TTTTGAGGAC CCCAGAGATG CCCCTCCACC CACAAGAGCA 
GAGACACGGG AGGAGAGACT GGAGAGGAAG AGAAGAGAAA AAATGGAGAG GAGGCAAGTT 
GTGGTTGAAG GAGAGCTGAA ATTATGGGAC CCACATAATG ACGCTAATGC CCAAGGAGAT 
GCGTTTAAGA CCCTGTTTGT GGCTCGAATT AATTATGATA CCACAGAATC CAAACTGCGG 
CGAGAGTTTG AGGTCTATGG TCCTATCAAA CGGATCTACA TTGTGTACAA CAAGAAAACT 
GGAAAACCAA GAGGCTATGC GTTCATTGAA TACGAGCACG AGAGAGACAT GCACTCTGCT 
TATAAGCATG CGGATGGCAA AAAGATCGAT GGTCGTAGAG TGCTTGTTGA TGTTGAAAGA 
GGACGAACGG TAAAAGGATG GCGCCCCCGG CGACTTGGTG GGGGTCTTGG TGGCACAAGG 
CGAGGAGGCG CAGATGTCAA CATCAAGCAC TCTGGCAGAG ACGACACATC TCGTTATGAT 
GACCGACCAA TTGGAAGTGA TCGCGATCGG GATCGTGGTG AACGCCGTGA ACGCAGTCGG 
GAACGTGATC GAGACAAGGA ACGTGGAGAA CGCAGACGTT CTCGGTCCAG AGAGAGACGC 
AGGCACACTA GGTCTCATGA GCGTGAGAGG GCCGCTGCAG GGGATGAGGC TGGGGGCAGC 
AGCCGACGAC GAGAGAGGGA AAGAGAACGA GGTGGTGCTG GAGCTGGAGA AAGCAGAGAG 
AGGAGTAGAG ACCGGGATCG AGATAGAGAC CGCAAGAGAA GGAGCAGGAG CAGGGATAGG 
AAGAGAGACA GGGAAAGAGG AAAAGGTGCA GAAGGAGGGG AGGAAGGCAT GGGCGGGCTA 
GTTGATGGCA TGATGCCTGA AGGGGGAGAT AGGGGCATAG AGGACCCCTT GGGTGGTGAA 
CCAGGGCGAG GTGAGGAGGG AGGACCTGAA GGAGAGGAAA GGGGGAGGGA TCGAGACAAA 
GACAGGGAAA GAGATCGTGA TAGGAAGCGC AGCCATCGAG ATAAAGATAG AGAAAGGGAT 
CGGGACAGAA GGCGGGACAG GGATAGAGAT CGTGACCATA AGCGAGACCG TGGAGATCGG 
GACCGTGGGG AGCGAAGGGA AGATCGACAC ATTACCTCCT CCGGAGATCA TGAGGGTTTG 
GGTAATGGAG GTGAAGAAGG TGAAGAGCCA GTGCCACCGC AATCAGAAGA AGGTTCACAG 
GATGGGATGA GGATGATGAT GATGGATCAG GATTCCATGC AGTCGGGAGA GGGCTATGCC 
TCCAATGAAA ATGGGTACAG GATGGAGGCC CAGGGTGATG AGTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003875.1
CDS	207..1694
Translation	MTQFLPPNLLALFAPRDPIPFLPQLGKLPHEKHHNQPYCGIAPFIRHFEDPRDAPPPTRAETREERLERKRREKMERRQVVVEGELKLWDPHNDANAQGDAFKTLFVARINYDTTESKLRREFEVYGPIKRIYIVYNKKTGKPRGYAFIEYEHERDMHSAYKHADGKKIDGRRVLVDVERGRTVKGWRPRRLGGGLGGTRRGGADVNIKHSGRDDTSRYDDRPIGSDRDRDRGERRERSRERDRDKERGERRRSRSRERRRHTRSHERERAAAGDEAGGSSRRRERERERGGAGAGESRERSRDRDRDRDRKRRSRSRDRKRDRERGKGAEGGEEGMGGLVDGMMPEGGDRGIEDPLGGEPGRGEEGGPEGEERGRDRDKDRERDRDRKRSHRDKDRERDRDRRRDRDRDRDHKRDRGDRDRGERREDRHITSSGDHEGLGNGGEEGEEPVPPQSEEGSQDGMRMMMMDQDSMQSGEGYASNENGYRMEAQGDEY

Target ORF information:

RefSeq Version	NM_001003875.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio small nuclear ribonucleoprotein 70 (U1) (snrnp70), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003875.2

ORF Insert Sequence:

ATGACTCAGT TTCTTCCGCC GAACCTGTTG GCATTGTTTG CCCCGCGGGA CCCCATACCG 
TTCCTGCCTC AACTGGGAAA GCTTCCTCAC GAGAAACACC ACAACCAGCC CTACTGCGGA 
ATTGCTCCTT TTATCCGGCA TTTTGAGGAC CCCAGAGATG CCCCTCCACC CACAAGAGCA 
GAGACACGGG AGGAGAGACT GGAGAGGAAG AGAAGAGAAA AAATGGAGAG GAGGCAAGTT 
GTGGTTGAAG GAGAGCTGAA ATTATGGGAC CCACATAATG ACGCTAATGC CCAAGGAGAT 
GCGTTTAAGA CCCTGTTTGT GGCTCGAATT AATTATGATA CCACAGAATC CAAACTGCGG 
CGAGAGTTTG AGGTCTATGG TCCTATCAAA CGGATCTACA TTGTGTACAA CAAGAAAACT 
GGAAAACCAA GAGGCTATGC GTTCATTGAA TACGAGCACG AGAGAGACAT GCACTCTGCT 
TATAAGCATG CGGATGGCAA AAAGATCGAT GGTCGTAGAG TGCTTGTTGA TGTTGAAAGA 
GGACGAACGG TAAAAGGATG GCGCCCCCGG CGACTTGGTG GGGGTCTTGG TGGCACAAGG 
CGAGGAGGCG CAGATGTCAA CATCAAGCAC TCTGGCAGAG ACGACACATC TCGTTATGAT 
GACCGACCAA TTGGAAGTGA TCGCGATCGG GATCGTGGTG AACGCCGTGA ACGCAGTCGG 
GAACGTGATC GAGACAAGGA ACGTGGAGAA CGCAGACGTT CTCGGTCCAG AGAGAGACGC 
AGGCACACTA GGTCTCATGA GCGTGAGAGG GCCGCTGCAG GGGATGAGGC TGGGGGCAGC 
AGCCGACGAC GAGAGAGGGA AAGAGAACGA GGTGGTGCTG GAGCTGGAGA AAGCAGAGAG 
AGGAGTAGAG ACCGGGATCG AGATAGAGAC CGCAAGAGAA GGAGCAGGAG CAGGGATAGG 
AAGAGAGACA GGGAAAGAGG AAAAGGTGCA GAAGGAGGGG AGGAAGGCAT GGGCGGGCTA 
GTTGATGGCA TGATGCCTGA AGGGGGAGAT AGGGGCATAG AGGACCCCTT GGGTGGTGAA 
CCAGGGCGAG GTGAGGAGGG AGGACCTGAA GGAGAGGAAA GGGGGAGGGA TCGAGACAAA 
GACAGGGAAA GAGATCGTGA TAGGAAGCGC AGCCATCGAG ATAAAGATAG AGAAAGGGAT 
CGGGACAGAA GGCGGGACAG GGATAGAGAT CGTGACCATA AGCGAGACCG TGGAGATCGG 
GACCGTGGGG AGCGAAGGGA AGATCGACAC ATTACCTCCT CCGGAGATCA TGAGGGTTTG 
GGTAATGGAG GTGAAGAAGG TGAAGAGCCA GTGCCACCGC AATCAGAAGA AGGTTCACAG 
GATGGGATGA GGATGATGAT GATGGATCAG GATTCCATGC AGTCGGGAGA GGGCTATGCC 
TCCAATGAAA ATGGGTACAG GATGGAGGCC CAGGGTGATG AGTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

snrnp70 ( XM_017354005.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209494.1 Danio rerio small nuclear ribonucleoprotein 70 (U1) (snrnp70), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa58036
Clone ID Related Accession (Same CDS sequence)	XM_017354005.1 , XM_017354005.2
Accession Version	XM_017354005.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	U1 small nuclear ribonucleoprotein 70 kDa isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCAGT TTCTTCCGCC GAACCTGTTG GCATTGTTTG CCCCGCGGGA CCCCATACCG 
TTCCTGCCTC AACTGGGAAA GCTTCCTCAC GAGAAACACC ACAACCAGCC CTACTGCGGA 
ATTGCTCCTT TTATCCGGCA TTTTGAGGAC CCCAGAGATG CCCCTCCACC CACAAGAGCA 
GAGACACGGG AGGAGAGACT GGAGAGGAAG AGAAGAGAAA AAATGGAGAG GAGGCAAGTT 
GTGGTTGAAG GAGAGCTGAA ATTATGGGAC CCACATAATG ACGCTAATGC CCAAGGAGAT 
GCGTTTAAGA CCCTGTTTGT GGCTCGAATT AATTATGATA CCACAGAATC CAAACTGCGG 
CGAGAGTTTG AGGTCTATGG TCCTATCAAA CGGATCTACA TTGTGTACAA CAAGAAAACT 
GGAAAACCAA GAGGCTATGC GTTCATTGAA TACGAGCACG AGAGAGACAT GCACTCCGCC 
TATCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209494.1
CDS	200..688
Translation	MTQFLPPNLLALFAPRDPIPFLPQLGKLPHEKHHNQPYCGIAPFIRHFEDPRDAPPPTRAETREERLERKRREKMERRQVVVEGELKLWDPHNDANAQGDAFKTLFVARINYDTTESKLRREFEVYGPIKRIYIVYNKKTGKPRGYAFIEYEHERDMHSAYL

Target ORF information:

RefSeq Version	XM_017354005.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio small nuclear ribonucleoprotein 70 (U1) (snrnp70), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354005.1

ORF Insert Sequence:

ATGACTCAGT TTCTTCCGCC GAACCTGTTG GCATTGTTTG CCCCGCGGGA CCCCATACCG 
TTCCTGCCTC AACTGGGAAA GCTTCCTCAC GAGAAACACC ACAACCAGCC CTACTGCGGA 
ATTGCTCCTT TTATCCGGCA TTTTGAGGAC CCCAGAGATG CCCCTCCACC CACAAGAGCA 
GAGACACGGG AGGAGAGACT GGAGAGGAAG AGAAGAGAAA AAATGGAGAG GAGGCAAGTT 
GTGGTTGAAG GAGAGCTGAA ATTATGGGAC CCACATAATG ACGCTAATGC CCAAGGAGAT 
GCGTTTAAGA CCCTGTTTGT GGCTCGAATT AATTATGATA CCACAGAATC CAAACTGCGG 
CGAGAGTTTG AGGTCTATGG TCCTATCAAA CGGATCTACA TTGTGTACAA CAAGAAAACT 
GGAAAACCAA GAGGCTATGC GTTCATTGAA TACGAGCACG AGAGAGACAT GCACTCCGCC 
TATCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

snrnp70 ( XM_017354005.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209494.1 Danio rerio small nuclear ribonucleoprotein 70 (U1) (snrnp70), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa58036
Clone ID Related Accession (Same CDS sequence)	XM_017354005.1 , XM_017354005.2
Accession Version	XM_017354005.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	U1 small nuclear ribonucleoprotein 70 kDa isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017354005.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCAGT TTCTTCCGCC GAACCTGTTG GCATTGTTTG CCCCGCGGGA CCCCATACCG 
TTCCTGCCTC AACTGGGAAA GCTTCCTCAC GAGAAACACC ACAACCAGCC CTACTGCGGA 
ATTGCTCCTT TTATCCGGCA TTTTGAGGAC CCCAGAGATG CCCCTCCACC CACAAGAGCA 
GAGACACGGG AGGAGAGACT GGAGAGGAAG AGAAGAGAAA AAATGGAGAG GAGGCAAGTT 
GTGGTTGAAG GAGAGCTGAA ATTATGGGAC CCACATAATG ACGCTAATGC CCAAGGAGAT 
GCGTTTAAGA CCCTGTTTGT GGCTCGAATT AATTATGATA CCACAGAATC CAAACTGCGG 
CGAGAGTTTG AGGTCTATGG TCCTATCAAA CGGATCTACA TTGTGTACAA CAAGAAAACT 
GGAAAACCAA GAGGCTATGC GTTCATTGAA TACGAGCACG AGAGAGACAT GCACTCCGCC 
TATCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209494.1
CDS	204..692
Translation	MTQFLPPNLLALFAPRDPIPFLPQLGKLPHEKHHNQPYCGIAPFIRHFEDPRDAPPPTRAETREERLERKRREKMERRQVVVEGELKLWDPHNDANAQGDAFKTLFVARINYDTTESKLRREFEVYGPIKRIYIVYNKKTGKPRGYAFIEYEHERDMHSAYL

Target ORF information:

RefSeq Version	XM_017354005.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio small nuclear ribonucleoprotein 70 (U1) (snrnp70), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354005.2

ORF Insert Sequence:

ATGACTCAGT TTCTTCCGCC GAACCTGTTG GCATTGTTTG CCCCGCGGGA CCCCATACCG 
TTCCTGCCTC AACTGGGAAA GCTTCCTCAC GAGAAACACC ACAACCAGCC CTACTGCGGA 
ATTGCTCCTT TTATCCGGCA TTTTGAGGAC CCCAGAGATG CCCCTCCACC CACAAGAGCA 
GAGACACGGG AGGAGAGACT GGAGAGGAAG AGAAGAGAAA AAATGGAGAG GAGGCAAGTT 
GTGGTTGAAG GAGAGCTGAA ATTATGGGAC CCACATAATG ACGCTAATGC CCAAGGAGAT 
GCGTTTAAGA CCCTGTTTGT GGCTCGAATT AATTATGATA CCACAGAATC CAAACTGCGG 
CGAGAGTTTG AGGTCTATGG TCCTATCAAA CGGATCTACA TTGTGTACAA CAAGAAAACT 
GGAAAACCAA GAGGCTATGC GTTCATTGAA TACGAGCACG AGAGAGACAT GCACTCCGCC 
TATCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Non-nuclear Pool of Splicing Factor SFPQ Regulates Axonal Transcripts Required for Normal Motor Development.
Neuron94(2)322-336.e5(2017 Apr)
Thomas-Jinu S,Gordon PM,Fielding T,Taylor R,Smith BN,Snowden V,Blanc E,Vance C,Topp S,Wong CH,Bielen H,Williams KL,McCann EP,Nicholson GA,Pan-Vazquez A,Fox AH,Bond CS,Talbot WS,Blair IP,Shaw CE,Houart C

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

U1 snRNP is mislocalized in ALS patient fibroblasts bearing NLS mutations in FUS and is required for motor neuron outgrowth in zebrafish.
Nucleic acids research43(6)3208-18(2015 Mar)
Yu Y,Chi B,Xia W,Gangopadhyay J,Yamazaki T,Winkelbauer-Hurt ME,Yin S,Eliasse Y,Adams E,Shaw CE,Reed R

RNA-Seq analysis in mutant zebrafish reveals role of U1C protein in alternative splicing regulation.
The EMBO journal30(10)1965-76(2011 May)
R?sel TD,Hung LH,Medenbach J,Donde K,Starke S,Benes V,R?tsch G,Bindereif A

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snrnp70 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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