ncaldb cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ncaldb
Entrez Gene ID	445319
Full Name	neurocalcin delta b
Synonyms	ncald,zgc:100982
General protein information	Preferred Names neurocalcin delta b Names neurocalcin-delta B
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001003776.1	NP_001003776.1	neurocalcin-delta B
XM_005159585.3	XP_005159642.1	neurocalcin-delta B isoform X1
XM_009294100.3	XP_009292375.1	neurocalcin-delta B isoform X1
XM_005159586.3	XP_005159643.1	neurocalcin-delta B isoform X1
XM_009294100.2	XP_009292375.1	neurocalcin-delta B isoform X1
XM_005159586.4	XP_005159643.1	neurocalcin-delta B isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Neurocalcin Delta Suppression Protects against Spinal Muscular Atrophy in Humans and across Species by Restoring Impaired Endocytosis.
Riessland M, Kaczmarek A, Schneider S, Swoboda KJ, L?hr H, Bradler C, Grysko V, Dimitriadi M, Hosseinibarkooie S, Torres-Benito L, Peters M, Upadhyay A, Biglari N, Kr?ber S, H?lker I, Garbes L, Gilissen C, Hoischen A, N?rnberg G, N?rnberg P, Walter M, Rigo F, Bennett CF, Kye MJ, Hart AC, Hammerschmidt M, Kloppenburg P, Wirth B
American journal of human genetics100(2)297-315(2017 Feb)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Global identification of SMAD2 target genes reveals a role for multiple co-regulatory factors in zebrafish early gastrulas.
Liu Z, Lin X, Cai Z, Zhang Z, Han C, Jia S, Meng A, Wang Q
The Journal of biological chemistry286(32)28520-32(2011 Aug)

Expressed sequence tag analysis of zebrafish eye tissues for NEIBank.
Vihtelic TS, Fadool JM, Gao J, Thornton KA, Hyde DR, Wistow G
Molecular vision111083-100(2005 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ncaldb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ncaldb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa38194	NM_001003776.1 Latest version!	Danio rerio neurocalcin delta b (ncaldb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
ODa23998	XM_009294100.3 Latest version!	Danio rerio neurocalcin delta b (ncaldb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
ODa23998	XM_005159586.4 Latest version!	Danio rerio neurocalcin delta b (ncaldb), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
ODa23998	XM_005159585.3 Latest version!	Danio rerio neurocalcin delta b (ncaldb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
View Old Accession Versions >>
ODa23998	XM_009294100.2	Danio rerio neurocalcin delta b (ncaldb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
ODa23998	XM_005159586.3	Danio rerio neurocalcin delta b (ncaldb), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ncaldb ( NM_001003776.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001003776.1 Danio rerio neurocalcin delta b (ncaldb), mRNA.

Price & Availability↑

CloneID	ODa38194
Clone ID Related Accession (Same CDS sequence)	NM_001003776.1
Accession Version	NM_001003776.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	neurocalcin-delta B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC079490.1. ##Evidence-Data-START## Transcript exon combination :: BC079490.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476756, SAMEA4476774 [ECO:0000348] ##Evidence-Data-END##

ATGGGAAAAC AAAACAGCAA GCTCCGTCCC GAGGTGATCC AGGATCTGCT GGACAACACA 
GATTTCACAG AGCATGAGAT CTTGGAGTGG TATAAGGGTT TTCTGCGGGA CTGTCCCAGC 
GGGGCGCTCA GCATGGACGA GTTCAAGAAG ATTTACGGCA ACTTCTTCCC CTATGGAGAC 
GCATCAAAGT TCGCAGAGCA TGTCTTCCGG ACCTTCGACG CCAATGGAGA TGGGACAATA 
GATTTCAGAG AGTTCATCAT TGCGCTGAGT GTAACATCAC GTGGCCGCTT GGATCAGAAG 
CTTAAATGGG CCTTCAGCAT GTACGATCTG GACGGCAACG GATACATCAG CAAGGCGGAG 
ATGCTGGAGA TTGTGCAGGC CATCTACAAG ATGGTATCTT CTGTGATGAA AATGCCAGAG 
GACGAGTCCA CACCTGAGAA GCGCACAGAC AAGATCTTCC GTCAGATGGA CACAAATCGT 
GATGGTAAGC TGTCACTCGA AGAGTTTGTA GAGGGTGCAA AGAACGACCC ATCCATCGTG 
CGACTTTTGC AGTGTGACCC CAGCAGCGCC GGTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003776.1
CDS	114..692
Translation	MGKQNSKLRPEVIQDLLDNTDFTEHEILEWYKGFLRDCPSGALSMDEFKKIYGNFFPYGDASKFAEHVFRTFDANGDGTIDFREFIIALSVTSRGRLDQKLKWAFSMYDLDGNGYISKAEMLEIVQAIYKMVSSVMKMPEDESTPEKRTDKIFRQMDTNRDGKLSLEEFVEGAKNDPSIVRLLQCDPSSAGQ

Target ORF information:

RefSeq Version	NM_001003776.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neurocalcin delta b (ncaldb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003776.1

ORF Insert Sequence:

ATGGGAAAAC AAAACAGCAA GCTCCGTCCC GAGGTGATCC AGGATCTGCT GGACAACACA 
GATTTCACAG AGCATGAGAT CTTGGAGTGG TATAAGGGTT TTCTGCGGGA CTGTCCCAGC 
GGGGCGCTCA GCATGGACGA GTTCAAGAAG ATTTACGGCA ACTTCTTCCC CTATGGAGAC 
GCATCAAAGT TCGCAGAGCA TGTCTTCCGG ACCTTCGACG CCAATGGAGA TGGGACAATA 
GATTTCAGAG AGTTCATCAT TGCGCTGAGT GTAACATCAC GTGGCCGCTT GGATCAGAAG 
CTTAAATGGG CCTTCAGCAT GTACGATCTG GACGGCAACG GATACATCAG CAAGGCGGAG 
ATGCTGGAGA TTGTGCAGGC CATCTACAAG ATGGTATCTT CTGTGATGAA AATGCCAGAG 
GACGAGTCCA CACCTGAGAA GCGCACAGAC AAGATCTTCC GTCAGATGGA CACAAATCGT 
GATGGTAAGC TGTCACTCGA AGAGTTTGTA GAGGGTGCAA AGAACGACCC ATCCATCGTG 
CGACTTTTGC AGTGTGACCC CAGCAGCGCC GGTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ncaldb ( XM_005159585.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159642.1 Danio rerio neurocalcin delta b (ncaldb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa23998
Clone ID Related Accession (Same CDS sequence)	XM_005159585.3 , XM_009294100.3 , XM_005159586.3 , XM_009294100.2 , XM_005159586.4
Accession Version	XM_005159585.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neurocalcin-delta B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159585.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAAAC AAAACAGCAA GCTCCGTCCC GAGGTGATCC AGGATCTGCT GGACAACACA 
GATTTCACAG AGCATGAGAT CTTGGAGTGG TATAAGGGTT TTCTGCGGGA CTGTCCCAGC 
GGGGCGCTCA GCATGGACGA GTTCAAGAAG ATCTACGGCA ACTTCTTCCC CTATGGAGAC 
GCATCAAAGT TCGCAGAGCA TGTCTTCCGG ACCTTCGACG CCAATGGAGA TGGGACAATA 
GATTTCAGAG AGTTCATCAT TGCGCTGAGT GTAACATCAC GTGGCCGCTT GGATCAGAAG 
CTTAAATGGG CCTTCAGCAT GTACGATCTA GACGGCAACG GATACATCAG CAAGGCGGAG 
ATGCTGGAGA TTGTGCAGGC CATCTACAAG ATGGTATCTT CTGTGATGAA AATGCCAGAG 
GACGAGTCCA CACCTGAGAA GCGCACAGAC AAGATCTTCC GTCAGATGGA CACAAATCGT 
GATGGTAAGC TGTCACTCGA AGAGTTTGTA GAGGGTGCAA AGAACGACCC ATCCATCGTG 
CGACTTTTGC AGTGTGACCC CAGCAGCGCC GGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159642.1
CDS	200..778
Translation	MGKQNSKLRPEVIQDLLDNTDFTEHEILEWYKGFLRDCPSGALSMDEFKKIYGNFFPYGDASKFAEHVFRTFDANGDGTIDFREFIIALSVTSRGRLDQKLKWAFSMYDLDGNGYISKAEMLEIVQAIYKMVSSVMKMPEDESTPEKRTDKIFRQMDTNRDGKLSLEEFVEGAKNDPSIVRLLQCDPSSAGQ

Target ORF information:

RefSeq Version	XM_005159585.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neurocalcin delta b (ncaldb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159585.3

ORF Insert Sequence:

ATGGGAAAAC AAAACAGCAA GCTCCGTCCC GAGGTGATCC AGGATCTGCT GGACAACACA 
GATTTCACAG AGCATGAGAT CTTGGAGTGG TATAAGGGTT TTCTGCGGGA CTGTCCCAGC 
GGGGCGCTCA GCATGGACGA GTTCAAGAAG ATCTACGGCA ACTTCTTCCC CTATGGAGAC 
GCATCAAAGT TCGCAGAGCA TGTCTTCCGG ACCTTCGACG CCAATGGAGA TGGGACAATA 
GATTTCAGAG AGTTCATCAT TGCGCTGAGT GTAACATCAC GTGGCCGCTT GGATCAGAAG 
CTTAAATGGG CCTTCAGCAT GTACGATCTA GACGGCAACG GATACATCAG CAAGGCGGAG 
ATGCTGGAGA TTGTGCAGGC CATCTACAAG ATGGTATCTT CTGTGATGAA AATGCCAGAG 
GACGAGTCCA CACCTGAGAA GCGCACAGAC AAGATCTTCC GTCAGATGGA CACAAATCGT 
GATGGTAAGC TGTCACTCGA AGAGTTTGTA GAGGGTGCAA AGAACGACCC ATCCATCGTG 
CGACTTTTGC AGTGTGACCC CAGCAGCGCC GGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ncaldb ( XM_009294100.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292375.1 Danio rerio neurocalcin delta b (ncaldb), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa23998
Clone ID Related Accession (Same CDS sequence)	XM_005159585.3 , XM_009294100.3 , XM_005159586.3 , XM_009294100.2 , XM_005159586.4
Accession Version	XM_009294100.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neurocalcin-delta B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294100.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAAAC AAAACAGCAA GCTCCGTCCC GAGGTGATCC AGGATCTGCT GGACAACACA 
GATTTCACAG AGCATGAGAT CTTGGAGTGG TATAAGGGTT TTCTGCGGGA CTGTCCCAGC 
GGGGCGCTCA GCATGGACGA GTTCAAGAAG ATCTACGGCA ACTTCTTCCC CTATGGAGAC 
GCATCAAAGT TCGCAGAGCA TGTCTTCCGG ACCTTCGACG CCAATGGAGA TGGGACAATA 
GATTTCAGAG AGTTCATCAT TGCGCTGAGT GTAACATCAC GTGGCCGCTT GGATCAGAAG 
CTTAAATGGG CCTTCAGCAT GTACGATCTA GACGGCAACG GATACATCAG CAAGGCGGAG 
ATGCTGGAGA TTGTGCAGGC CATCTACAAG ATGGTATCTT CTGTGATGAA AATGCCAGAG 
GACGAGTCCA CACCTGAGAA GCGCACAGAC AAGATCTTCC GTCAGATGGA CACAAATCGT 
GATGGTAAGC TGTCACTCGA AGAGTTTGTA GAGGGTGCAA AGAACGACCC ATCCATCGTG 
CGACTTTTGC AGTGTGACCC CAGCAGCGCC GGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292375.1
CDS	238..816
Translation	MGKQNSKLRPEVIQDLLDNTDFTEHEILEWYKGFLRDCPSGALSMDEFKKIYGNFFPYGDASKFAEHVFRTFDANGDGTIDFREFIIALSVTSRGRLDQKLKWAFSMYDLDGNGYISKAEMLEIVQAIYKMVSSVMKMPEDESTPEKRTDKIFRQMDTNRDGKLSLEEFVEGAKNDPSIVRLLQCDPSSAGQ

Target ORF information:

RefSeq Version	XM_009294100.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neurocalcin delta b (ncaldb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294100.3

ORF Insert Sequence:

ATGGGAAAAC AAAACAGCAA GCTCCGTCCC GAGGTGATCC AGGATCTGCT GGACAACACA 
GATTTCACAG AGCATGAGAT CTTGGAGTGG TATAAGGGTT TTCTGCGGGA CTGTCCCAGC 
GGGGCGCTCA GCATGGACGA GTTCAAGAAG ATCTACGGCA ACTTCTTCCC CTATGGAGAC 
GCATCAAAGT TCGCAGAGCA TGTCTTCCGG ACCTTCGACG CCAATGGAGA TGGGACAATA 
GATTTCAGAG AGTTCATCAT TGCGCTGAGT GTAACATCAC GTGGCCGCTT GGATCAGAAG 
CTTAAATGGG CCTTCAGCAT GTACGATCTA GACGGCAACG GATACATCAG CAAGGCGGAG 
ATGCTGGAGA TTGTGCAGGC CATCTACAAG ATGGTATCTT CTGTGATGAA AATGCCAGAG 
GACGAGTCCA CACCTGAGAA GCGCACAGAC AAGATCTTCC GTCAGATGGA CACAAATCGT 
GATGGTAAGC TGTCACTCGA AGAGTTTGTA GAGGGTGCAA AGAACGACCC ATCCATCGTG 
CGACTTTTGC AGTGTGACCC CAGCAGCGCC GGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ncaldb ( XM_005159586.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159643.1 Danio rerio neurocalcin delta b (ncaldb), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa23998
Clone ID Related Accession (Same CDS sequence)	XM_005159585.3 , XM_009294100.3 , XM_005159586.3 , XM_009294100.2 , XM_005159586.4
Accession Version	XM_005159586.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	neurocalcin-delta B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159586.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAAAC AAAACAGCAA GCTCCGTCCC GAGGTGATCC AGGATCTGCT GGACAACACA 
GATTTCACAG AGCATGAGAT CTTGGAGTGG TATAAGGGTT TTCTGCGGGA CTGTCCCAGC 
GGGGCGCTCA GCATGGACGA GTTCAAGAAG ATCTACGGCA ACTTCTTCCC CTATGGAGAC 
GCATCAAAGT TCGCAGAGCA TGTCTTCCGG ACCTTCGACG CCAATGGAGA TGGGACAATA 
GATTTCAGAG AGTTCATCAT TGCGCTGAGT GTAACATCAC GTGGCCGCTT GGATCAGAAG 
CTTAAATGGG CCTTCAGCAT GTACGATCTA GACGGCAACG GATACATCAG CAAGGCGGAG 
ATGCTGGAGA TTGTGCAGGC CATCTACAAG ATGGTATCTT CTGTGATGAA AATGCCAGAG 
GACGAGTCCA CACCTGAGAA GCGCACAGAC AAGATCTTCC GTCAGATGGA CACAAATCGT 
GATGGTAAGC TGTCACTCGA AGAGTTTGTA GAGGGTGCAA AGAACGACCC ATCCATCGTG 
CGACTTTTGC AGTGTGACCC CAGCAGCGCC GGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159643.1
CDS	327..905
Translation	MGKQNSKLRPEVIQDLLDNTDFTEHEILEWYKGFLRDCPSGALSMDEFKKIYGNFFPYGDASKFAEHVFRTFDANGDGTIDFREFIIALSVTSRGRLDQKLKWAFSMYDLDGNGYISKAEMLEIVQAIYKMVSSVMKMPEDESTPEKRTDKIFRQMDTNRDGKLSLEEFVEGAKNDPSIVRLLQCDPSSAGQ

Target ORF information:

RefSeq Version	XM_005159586.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio neurocalcin delta b (ncaldb), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159586.3

ORF Insert Sequence:

ATGGGAAAAC AAAACAGCAA GCTCCGTCCC GAGGTGATCC AGGATCTGCT GGACAACACA 
GATTTCACAG AGCATGAGAT CTTGGAGTGG TATAAGGGTT TTCTGCGGGA CTGTCCCAGC 
GGGGCGCTCA GCATGGACGA GTTCAAGAAG ATCTACGGCA ACTTCTTCCC CTATGGAGAC 
GCATCAAAGT TCGCAGAGCA TGTCTTCCGG ACCTTCGACG CCAATGGAGA TGGGACAATA 
GATTTCAGAG AGTTCATCAT TGCGCTGAGT GTAACATCAC GTGGCCGCTT GGATCAGAAG 
CTTAAATGGG CCTTCAGCAT GTACGATCTA GACGGCAACG GATACATCAG CAAGGCGGAG 
ATGCTGGAGA TTGTGCAGGC CATCTACAAG ATGGTATCTT CTGTGATGAA AATGCCAGAG 
GACGAGTCCA CACCTGAGAA GCGCACAGAC AAGATCTTCC GTCAGATGGA CACAAATCGT 
GATGGTAAGC TGTCACTCGA AGAGTTTGTA GAGGGTGCAA AGAACGACCC ATCCATCGTG 
CGACTTTTGC AGTGTGACCC CAGCAGCGCC GGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ncaldb ( XM_009294100.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292375.1 Danio rerio neurocalcin delta b (ncaldb), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa23998
Clone ID Related Accession (Same CDS sequence)	XM_005159585.3 , XM_009294100.3 , XM_005159586.3 , XM_009294100.2 , XM_005159586.4
Accession Version	XM_009294100.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	neurocalcin-delta B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294100.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAAAC AAAACAGCAA GCTCCGTCCC GAGGTGATCC AGGATCTGCT GGACAACACA 
GATTTCACAG AGCATGAGAT CTTGGAGTGG TATAAGGGTT TTCTGCGGGA CTGTCCCAGC 
GGGGCGCTCA GCATGGACGA GTTCAAGAAG ATCTACGGCA ACTTCTTCCC CTATGGAGAC 
GCATCAAAGT TCGCAGAGCA TGTCTTCCGG ACCTTCGACG CCAATGGAGA TGGGACAATA 
GATTTCAGAG AGTTCATCAT TGCGCTGAGT GTAACATCAC GTGGCCGCTT GGATCAGAAG 
CTTAAATGGG CCTTCAGCAT GTACGATCTA GACGGCAACG GATACATCAG CAAGGCGGAG 
ATGCTGGAGA TTGTGCAGGC CATCTACAAG ATGGTATCTT CTGTGATGAA AATGCCAGAG 
GACGAGTCCA CACCTGAGAA GCGCACAGAC AAGATCTTCC GTCAGATGGA CACAAATCGT 
GATGGTAAGC TGTCACTCGA AGAGTTTGTA GAGGGTGCAA AGAACGACCC ATCCATCGTG 
CGACTTTTGC AGTGTGACCC CAGCAGCGCC GGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292375.1
CDS	237..815
Translation	MGKQNSKLRPEVIQDLLDNTDFTEHEILEWYKGFLRDCPSGALSMDEFKKIYGNFFPYGDASKFAEHVFRTFDANGDGTIDFREFIIALSVTSRGRLDQKLKWAFSMYDLDGNGYISKAEMLEIVQAIYKMVSSVMKMPEDESTPEKRTDKIFRQMDTNRDGKLSLEEFVEGAKNDPSIVRLLQCDPSSAGQ

Target ORF information:

RefSeq Version	XM_009294100.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio neurocalcin delta b (ncaldb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294100.2

ORF Insert Sequence:

ATGGGAAAAC AAAACAGCAA GCTCCGTCCC GAGGTGATCC AGGATCTGCT GGACAACACA 
GATTTCACAG AGCATGAGAT CTTGGAGTGG TATAAGGGTT TTCTGCGGGA CTGTCCCAGC 
GGGGCGCTCA GCATGGACGA GTTCAAGAAG ATCTACGGCA ACTTCTTCCC CTATGGAGAC 
GCATCAAAGT TCGCAGAGCA TGTCTTCCGG ACCTTCGACG CCAATGGAGA TGGGACAATA 
GATTTCAGAG AGTTCATCAT TGCGCTGAGT GTAACATCAC GTGGCCGCTT GGATCAGAAG 
CTTAAATGGG CCTTCAGCAT GTACGATCTA GACGGCAACG GATACATCAG CAAGGCGGAG 
ATGCTGGAGA TTGTGCAGGC CATCTACAAG ATGGTATCTT CTGTGATGAA AATGCCAGAG 
GACGAGTCCA CACCTGAGAA GCGCACAGAC AAGATCTTCC GTCAGATGGA CACAAATCGT 
GATGGTAAGC TGTCACTCGA AGAGTTTGTA GAGGGTGCAA AGAACGACCC ATCCATCGTG 
CGACTTTTGC AGTGTGACCC CAGCAGCGCC GGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ncaldb ( XM_005159586.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159643.1 Danio rerio neurocalcin delta b (ncaldb), transcript variant X3, mRNA.

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CloneID	ODa23998
Clone ID Related Accession (Same CDS sequence)	XM_005159585.3 , XM_009294100.3 , XM_005159586.3 , XM_009294100.2 , XM_005159586.4
Accession Version	XM_005159586.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neurocalcin-delta B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159586.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAAAC AAAACAGCAA GCTCCGTCCC GAGGTGATCC AGGATCTGCT GGACAACACA 
GATTTCACAG AGCATGAGAT CTTGGAGTGG TATAAGGGTT TTCTGCGGGA CTGTCCCAGC 
GGGGCGCTCA GCATGGACGA GTTCAAGAAG ATCTACGGCA ACTTCTTCCC CTATGGAGAC 
GCATCAAAGT TCGCAGAGCA TGTCTTCCGG ACCTTCGACG CCAATGGAGA TGGGACAATA 
GATTTCAGAG AGTTCATCAT TGCGCTGAGT GTAACATCAC GTGGCCGCTT GGATCAGAAG 
CTTAAATGGG CCTTCAGCAT GTACGATCTA GACGGCAACG GATACATCAG CAAGGCGGAG 
ATGCTGGAGA TTGTGCAGGC CATCTACAAG ATGGTATCTT CTGTGATGAA AATGCCAGAG 
GACGAGTCCA CACCTGAGAA GCGCACAGAC AAGATCTTCC GTCAGATGGA CACAAATCGT 
GATGGTAAGC TGTCACTCGA AGAGTTTGTA GAGGGTGCAA AGAACGACCC ATCCATCGTG 
CGACTTTTGC AGTGTGACCC CAGCAGCGCC GGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159643.1
CDS	161..739
Translation	MGKQNSKLRPEVIQDLLDNTDFTEHEILEWYKGFLRDCPSGALSMDEFKKIYGNFFPYGDASKFAEHVFRTFDANGDGTIDFREFIIALSVTSRGRLDQKLKWAFSMYDLDGNGYISKAEMLEIVQAIYKMVSSVMKMPEDESTPEKRTDKIFRQMDTNRDGKLSLEEFVEGAKNDPSIVRLLQCDPSSAGQ

Target ORF information:

RefSeq Version	XM_005159586.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neurocalcin delta b (ncaldb), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159586.4

ORF Insert Sequence:

ATGGGAAAAC AAAACAGCAA GCTCCGTCCC GAGGTGATCC AGGATCTGCT GGACAACACA 
GATTTCACAG AGCATGAGAT CTTGGAGTGG TATAAGGGTT TTCTGCGGGA CTGTCCCAGC 
GGGGCGCTCA GCATGGACGA GTTCAAGAAG ATCTACGGCA ACTTCTTCCC CTATGGAGAC 
GCATCAAAGT TCGCAGAGCA TGTCTTCCGG ACCTTCGACG CCAATGGAGA TGGGACAATA 
GATTTCAGAG AGTTCATCAT TGCGCTGAGT GTAACATCAC GTGGCCGCTT GGATCAGAAG 
CTTAAATGGG CCTTCAGCAT GTACGATCTA GACGGCAACG GATACATCAG CAAGGCGGAG 
ATGCTGGAGA TTGTGCAGGC CATCTACAAG ATGGTATCTT CTGTGATGAA AATGCCAGAG 
GACGAGTCCA CACCTGAGAA GCGCACAGAC AAGATCTTCC GTCAGATGGA CACAAATCGT 
GATGGTAAGC TGTCACTCGA AGAGTTTGTA GAGGGTGCAA AGAACGACCC ATCCATCGTG 
CGACTTTTGC AGTGTGACCC CAGCAGCGCC GGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Neurocalcin Delta Suppression Protects against Spinal Muscular Atrophy in Humans and across Species by Restoring Impaired Endocytosis.
American journal of human genetics100(2)297-315(2017 Feb)
Riessland M,Kaczmarek A,Schneider S,Swoboda KJ,L?hr H,Bradler C,Grysko V,Dimitriadi M,Hosseinibarkooie S,Torres-Benito L,Peters M,Upadhyay A,Biglari N,Kr?ber S,H?lker I,Garbes L,Gilissen C,Hoischen A,N?rnberg G,N?rnberg P,Walter M,Rigo F,Bennett CF,Kye MJ,Hart AC,Hammerschmidt M,Kloppenburg P,Wirth B

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Global identification of SMAD2 target genes reveals a role for multiple co-regulatory factors in zebrafish early gastrulas.
The Journal of biological chemistry286(32)28520-32(2011 Aug)
Liu Z,Lin X,Cai Z,Zhang Z,Han C,Jia S,Meng A,Wang Q

Expressed sequence tag analysis of zebrafish eye tissues for NEIBank.
Molecular vision111083-100(2005 Dec)
Vihtelic TS,Fadool JM,Gao J,Thornton KA,Hyde DR,Wistow G

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ncaldb cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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