npsn cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	npsn
Entrez Gene ID	404039
Full Name	nephrosin
Synonyms	zgc:110470,zgc:152831
General protein information	Preferred Names nephrosin Names nephrosin ZC6AST2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_205756.3	NP_991319.2	nephrosin isoform 2 precursor
NM_001077779.2	NP_001071247.1	nephrosin isoform 1 precursor

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	c6ast4	NP_001020351.1
Xenopus tropicalis (tropical clawed frog)	LOC100489456	XP_002932488.1
Danio rerio (zebrafish)	npsn	NP_001071247.1
Danio rerio (zebrafish)	c6ast3	NP_001013544.1
Danio rerio (zebrafish)	c6ast1	NP_001036784.1

Gene Ontology
Bibliography

Related articles in PubMed

Zebrafish nephrosin helps host defence against
Di Q, Lin Q, Huang Z, Chi Y, Chen X, Zhang W, Zhang Y
Open biology7(8)(2017 Aug)

The seahorse genome and the evolution of its specialized morphology.
Lin Q, Fan S, Zhang Y, Xu M, Zhang H, Yang Y, Lee AP, Woltering JM, Ravi V, Gunter HM, Luo W, Gao Z, Lim ZW, Qin G, Schneider RF, Wang X, Xiong P, Li G, Wang K, Min J, Zhang C, Qiu Y, Bai J, He W, Bian C, Zhang X, Shan D, Qu H, Sun Y, Gao Q, Huang L, Shi Q, Meyer A, Venkatesh B
Nature540(7633)395-399(2016 12)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

NACA deficiency reveals the crucial role of somite-derived stromal cells in haematopoietic niche formation.
Murayama E, Sarris M, Redd M, Le Guyader D, Vivier C, Horsley W, Trede N, Herbomel P
Nature communications68375(2015 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following npsn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the npsn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa38000	NM_001077779.2 Latest version!	Danio rerio nephrosin (npsn), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
ODa53256	NM_205756.3 Latest version!	Danio rerio nephrosin (npsn), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

npsn ( NM_001077779.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001071247.1 Danio rerio nephrosin (npsn), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ODa38000
Clone ID Related Accession (Same CDS sequence)	NM_001077779.2
Accession Version	NM_001077779.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	nephrosin isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX957297.10 and EH434994.1. On or before Mar 30, 2018 this sequence version replaced NM_205582.1, NM_001077779.1. Transcript Variant: This variant (1) encodes isoform 1. Sequence Note: This RefSeq record was created from genomic sequence data because no single transcript from the reference genome strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC124146.1, BC095288.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTACCTGT TGGTAGTGGT CATCTCTCTT CTGCTGAGCT CTGGTCCTGT TCAGAGCCGT 
CCTGTTGAGG ATTTAATTGA AAAAATGTCC AAGAAGACTG GTGGTAACTT CACAAAGAAA 
GGTAATTTGC TAGTCTCATC TGTAATCGAG ACCAGCAAAC ATGCAGGAGA GAACACAGAT 
GGGCCAGTAA TCCTGTTTGG AGACATCGCC TTTCCCAGAG GTTTGCAGAA TGCAGATCAA 
TGTACAGCTC GTGGCTGCAA ATGGGACAGA AGCAGAGATG GATTAGTCTA TGTGCCTTAC 
CAGATATCTA GAGCGTACTC TCCACGGGAA GTAGCAGTGA TTGAACAAGG CTTGCAATCC 
TTTTCGGCGG TTTCCTGCAT TCGTTTTGTG CCTCATACTG GAGAGAGGAA CTATCTCAAC 
ATAAAGTCTG AATCTGGCTG CTATTCATAT TTAGGGCGGA TAGGTGGAGG TCAGGTCGTG 
TCTCTCCAGC GGCAAGGCTG TGTGTATTTC AGCACCGTCC AGCACGAGCT CCTTCATGCC 
CTCGGCTTCC ACCATGAGCA GAACCGCAGC GACCGAGACA ATCACATCCG GATCCTCTAT 
CAGAACATCA TACCTGCGCA ACAGTACAAT TTCAACAAAC AAAACACCAA CAATCTGGGC 
ACCCCCTATG ACTATAACTC TGTGATGCAG TATTCAAGGT ATGCTTTCTC CATGAACAAT 
CAGCCAACCA TGGTTCCCGT TCCTAATGCA AATGTTGTGT TGGGAGAAGC TCAAAGTATG 
AGCCCCAATG ACATCCTGCG CATCAACAGA CTATACTGCA ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001071247.1
CDS	25..849
Translation	MYLLVVVISLLLSSGPVQSRPVEDLIEKMSKKTGGNFTKKGNLLVSSVIETSKHAGENTDGPVILFGDIAFPRGLQNADQCTARGCKWDRSRDGLVYVPYQISRAYSPREVAVIEQGLQSFSAVSCIRFVPHTGERNYLNIKSESGCYSYLGRIGGGQVVSLQRQGCVYFSTVQHELLHALGFHHEQNRSDRDNHIRILYQNIIPAQQYNFNKQNTNNLGTPYDYNSVMQYSRYAFSMNNQPTMVPVPNANVVLGEAQSMSPNDILRINRLYCN

Target ORF information:

RefSeq Version	NM_001077779.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nephrosin (npsn), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001077779.2

ORF Insert Sequence:

ATGTACCTGT TGGTAGTGGT CATCTCTCTT CTGCTGAGCT CTGGTCCTGT TCAGAGCCGT 
CCTGTTGAGG ATTTAATTGA AAAAATGTCC AAGAAGACTG GTGGTAACTT CACAAAGAAA 
GGTAATTTGC TAGTCTCATC TGTAATCGAG ACCAGCAAAC ATGCAGGAGA GAACACAGAT 
GGGCCAGTAA TCCTGTTTGG AGACATCGCC TTTCCCAGAG GTTTGCAGAA TGCAGATCAA 
TGTACAGCTC GTGGCTGCAA ATGGGACAGA AGCAGAGATG GATTAGTCTA TGTGCCTTAC 
CAGATATCTA GAGCGTACTC TCCACGGGAA GTAGCAGTGA TTGAACAAGG CTTGCAATCC 
TTTTCGGCGG TTTCCTGCAT TCGTTTTGTG CCTCATACTG GAGAGAGGAA CTATCTCAAC 
ATAAAGTCTG AATCTGGCTG CTATTCATAT TTAGGGCGGA TAGGTGGAGG TCAGGTCGTG 
TCTCTCCAGC GGCAAGGCTG TGTGTATTTC AGCACCGTCC AGCACGAGCT CCTTCATGCC 
CTCGGCTTCC ACCATGAGCA GAACCGCAGC GACCGAGACA ATCACATCCG GATCCTCTAT 
CAGAACATCA TACCTGCGCA ACAGTACAAT TTCAACAAAC AAAACACCAA CAATCTGGGC 
ACCCCCTATG ACTATAACTC TGTGATGCAG TATTCAAGGT ATGCTTTCTC CATGAACAAT 
CAGCCAACCA TGGTTCCCGT TCCTAATGCA AATGTTGTGT TGGGAGAAGC TCAAAGTATG 
AGCCCCAATG ACATCCTGCG CATCAACAGA CTATACTGCA ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

npsn ( NM_205756.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_991319.2 Danio rerio nephrosin (npsn), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ODa53256
Clone ID Related Accession (Same CDS sequence)	NM_205756.3
Accession Version	NM_205756.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	nephrosin isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX957297.10. On Feb 4, 2016 this sequence version replaced NM_205756.2. Transcript Variant: This variant (2) lacks the 3' terminal exon and extends past a splice site that is used in variant 1. This results in a novel 3' coding region and 3' UTR, compared to variant 1. It encodes isoform 2 which is longer and has a distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from genomic sequence data because no single transcript from the reference genome strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF498291.1, CN015480.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTACCTGT TGGTAGTGGT CATCTCTCTT CTGCTGAGCT CTGGTCCTGT TCAGAGCCGT 
CCTGTTGAGG ATTTAATTGA AAAAATGTCC AAGAAGACTG GTGGTAACTT CACAAAGAAA 
GGTAATTTGC TAGTCTCATC TGTAATCGAG ACCAGCAAAC ATGCAGGAGA GAACACAGAT 
GGGCCAGTAA TCCTGTTTGG AGACATCGCC TTTCCCAGAG GTTTGCAGAA TGCAGATCAA 
TGTACAGCTC GTGGCTGCAA ATGGGACAGA AGCAGAGATG GATTAGTCTA TGTGCCTTAC 
CAGATATCTA GAGCGTACTC TCCACGGGAA GTAGCAGTGA TTGAACAAGG CTTGCAATCC 
TTTTCGGCGG TTTCCTGCAT TCGTTTTGTG CCTCATACTG GAGAGAGGAA CTATCTCAAC 
ATAAAGTCTG AATCTGGCTG CTATTCATAT TTAGGGCGGA TAGGTGGAGG TCAGGTCGTG 
TCTCTCCAGC GGCAAGGCTG TGTGTATTTC AGCACCGTCC AGCACGAGCT CCTTCATGCC 
CTCGGCTTCC ACCATGAGCA GAACCGCAGC GACCGAGACA ATCACATCCG GATCCTCTAT 
CAGAACATCA TACCTGCGCA ACAGTACAAT TTCAACAAAC AAAACACCAA CAATCTGGGC 
ACCCCCTATG ACTATAACTC TGTGATGCAG TATTCAAGGT ATGCTTTCTC CATGAACAAT 
CAGCCAACCA TGGTTCCCGT TCCTAATGCA AATGTTGTGT TGGGAGAAGC TCAAAGTATG 
AGCCCCAATG ACATCCTGCG CATCAACAGA CTATACTGCA GTACGTTCAA CATTTCCTAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_991319.2
CDS	25..867
Translation	MYLLVVVISLLLSSGPVQSRPVEDLIEKMSKKTGGNFTKKGNLLVSSVIETSKHAGENTDGPVILFGDIAFPRGLQNADQCTARGCKWDRSRDGLVYVPYQISRAYSPREVAVIEQGLQSFSAVSCIRFVPHTGERNYLNIKSESGCYSYLGRIGGGQVVSLQRQGCVYFSTVQHELLHALGFHHEQNRSDRDNHIRILYQNIIPAQQYNFNKQNTNNLGTPYDYNSVMQYSRYAFSMNNQPTMVPVPNANVVLGEAQSMSPNDILRINRLYCSTFNISY

Target ORF information:

RefSeq Version	NM_205756.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nephrosin (npsn), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205756.3

ORF Insert Sequence:

ATGTACCTGT TGGTAGTGGT CATCTCTCTT CTGCTGAGCT CTGGTCCTGT TCAGAGCCGT 
CCTGTTGAGG ATTTAATTGA AAAAATGTCC AAGAAGACTG GTGGTAACTT CACAAAGAAA 
GGTAATTTGC TAGTCTCATC TGTAATCGAG ACCAGCAAAC ATGCAGGAGA GAACACAGAT 
GGGCCAGTAA TCCTGTTTGG AGACATCGCC TTTCCCAGAG GTTTGCAGAA TGCAGATCAA 
TGTACAGCTC GTGGCTGCAA ATGGGACAGA AGCAGAGATG GATTAGTCTA TGTGCCTTAC 
CAGATATCTA GAGCGTACTC TCCACGGGAA GTAGCAGTGA TTGAACAAGG CTTGCAATCC 
TTTTCGGCGG TTTCCTGCAT TCGTTTTGTG CCTCATACTG GAGAGAGGAA CTATCTCAAC 
ATAAAGTCTG AATCTGGCTG CTATTCATAT TTAGGGCGGA TAGGTGGAGG TCAGGTCGTG 
TCTCTCCAGC GGCAAGGCTG TGTGTATTTC AGCACCGTCC AGCACGAGCT CCTTCATGCC 
CTCGGCTTCC ACCATGAGCA GAACCGCAGC GACCGAGACA ATCACATCCG GATCCTCTAT 
CAGAACATCA TACCTGCGCA ACAGTACAAT TTCAACAAAC AAAACACCAA CAATCTGGGC 
ACCCCCTATG ACTATAACTC TGTGATGCAG TATTCAAGGT ATGCTTTCTC CATGAACAAT 
CAGCCAACCA TGGTTCCCGT TCCTAATGCA AATGTTGTGT TGGGAGAAGC TCAAAGTATG 
AGCCCCAATG ACATCCTGCG CATCAACAGA CTATACTGCA GTACGTTCAA CATTTCCTAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Zebrafish nephrosin helps host defence against
Open biology7(8)(2017 Aug)
Di Q,Lin Q,Huang Z,Chi Y,Chen X,Zhang W,Zhang Y

The seahorse genome and the evolution of its specialized morphology.
Nature540(7633)395-399(2016 12)
Lin Q,Fan S,Zhang Y,Xu M,Zhang H,Yang Y,Lee AP,Woltering JM,Ravi V,Gunter HM,Luo W,Gao Z,Lim ZW,Qin G,Schneider RF,Wang X,Xiong P,Li G,Wang K,Min J,Zhang C,Qiu Y,Bai J,He W,Bian C,Zhang X,Shan D,Qu H,Sun Y,Gao Q,Huang L,Shi Q,Meyer A,Venkatesh B

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

NACA deficiency reveals the crucial role of somite-derived stromal cells in haematopoietic niche formation.
Nature communications68375(2015 Sep)
Murayama E,Sarris M,Redd M,Le Guyader D,Vivier C,Horsley W,Trede N,Herbomel P

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npsn cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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