rapgef1b cDNA ORF clone, Danio rerio(zebrafish)

The following rapgef1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rapgef1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa150861 XM_021468780.1
Latest version!
Danio rerio Rap guanine nucleotide exchange factor (GEF) 1b (rapgef1b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa150862 XM_021468781.1
Latest version!
Danio rerio Rap guanine nucleotide exchange factor (GEF) 1b (rapgef1b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa150863 XM_021468782.1
Latest version!
Danio rerio Rap guanine nucleotide exchange factor (GEF) 1b (rapgef1b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa150864 XM_021468783.1
Latest version!
Danio rerio Rap guanine nucleotide exchange factor (GEF) 1b (rapgef1b), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa150865 XM_021468784.1
Latest version!
Danio rerio Rap guanine nucleotide exchange factor (GEF) 1b (rapgef1b), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa56768 XM_017352761.1
Latest version!
Danio rerio Rap guanine nucleotide exchange factor (GEF) 1b (rapgef1b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa40639 NM_001328346.1
Latest version!
Danio rerio Rap guanine nucleotide exchange factor (GEF) 1b (rapgef1b), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa40640 XM_009295118.2
Latest version!
Danio rerio Rap guanine nucleotide exchange factor (GEF) 1b (rapgef1b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa40641 XM_009295119.2
Latest version!
Danio rerio Rap guanine nucleotide exchange factor (GEF) 1b (rapgef1b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa40642 XM_009295120.2
Latest version!
Danio rerio Rap guanine nucleotide exchange factor (GEF) 1b (rapgef1b), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa150861
    Clone ID Related Accession (Same CDS sequence) XM_021468780.1
    Accession Version XM_021468780.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3615bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rap guanine nucleotide exchange factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGGAACA GCAGTCGGAG AACTCAGCCT CTTCTGGAGG ACACGGACTA CTTTAATCCT 
    GGAGAAGCAG ATACAGAATC CCAGAAGTCT CACCTGTCGT CCTTCACGAT GAAACTGATG
    AAGTTTCATT CCCCGAAGAT TAAACGGACC CCATCTAAGA AAGGCAAACA ACTCCAGCCT
    GAGCCTGCAG TGAAAACGCC AGAGAAGCCA GTCAACAAGA AGGTGAGTCG ACTGGAGGAA
    CAGGAGAAGG ATGTGGTCAG CGCTCTGAGA TACTTTAAGA CGATTGTGGA CAAGATGAAC
    GTTGACACCA AAGTTCTCCA GATGCTTCCT GGTTCGGCTA GTAAAGTTTT AGAGGCTATT
    TTGCCTCTGA TGCAGGTCGA GGCTCGGATA CAGCACAGCT CCGCCATGTC CTCCTGTCAC
    AACCGTGTTT ATCAGAGCTT GGCGACTCTG ATTCGCTGGT CCGACCAGGT GATGCTGGAG
    GGCATCGACC TCGACGACAA GGACACTGTG ACGACTGTTA CCACGGTGAT CAAAGCTGTG
    CTGGATGGTG TGAAGGAGCT TGTGAAACTC ACTATAGAGA AACATGACCA ACCGTCCCCA
    ACATCCCCCA GCAAACCAGC GCCTCCTGTG GCCAAAACAG AGAGTGGGTG TGAAAATCCT
    CTAACCGAAA GAGAGAAGGC CATCCTGAGT AAGACCACGC CCATTGTCAC GCCCACTGAC
    AGCTTGACTG ACATGTCAGA GGAGGAGGCT CCACCCAAAC CTCCTCTACC AGGACCCAAA
    CTAGCAGAGC ACAGGAACAG GATGCAGTTG TGCTCCGATC CTGTTACCGG GCAGCGGAGG
    GCTACGATGA TGGAAACTCC ACCGGCTCTG CCTCCGAAGA AGCGTCAGTC GGCCCCGTCA
    CCCACCCGTG TGGCAGTGGT GGCCCCGATG AGCCGGGGTC CGTGTAGCCT CAGCCTGCCT
    CCTGGAGCCC TCAGACAGCA GCAGGACTTT GATGTGGATC TCCTCCAGAG GCGTTTCTCA
    GGCGGCAGTC AGTCATATGA TGGAGAATCT CCTCGTCTTT CTCCCTGTAA CAGCATGGGA
    AAACTCAGCA AATCTGATGA GCAGCTTTCG TCTCTGGAGC GGGACAGTGG ACAGTGCTCT
    CGCAACACCA GCTGTGAAAC GCTTGAGGGA TACGATCCAG ATTACGACTT TTTAAATCAG
    GATCTGTCCG TCTCGGAACC GCTGCCGTTT CCCACCAACA CTGGCGGCAG TCTGAGCCCT
    CTTCCAGAGT GCAACACTGA GTCTCAATCT CTAAGCCCCG CCCACATCCC TGCACGCCCA
    CGCTTCAGCG CTCCAGTAAC CAATCAGGCC TCCCTAGACT ACTGGACTCC ACCCCTCACA
    CCTGGACAGG GTAATAGCGT GCTTCCCCCT GCCCTCCCAC AGAAGAAGCG CCGCTCTGCA
    CCAATCATAT CGGCGTATCC TCTGTACGAG CGCCTCCCGT CACACTACGA TAACCTATCA
    GAGGAGGAGC AGCCGACGCC GCCGTTTCCA CTGGTTACAC CGGGGTCGCC GCAGGTCAAC
    GGAGACGGTG TTTTACAGTA CACAGACGCT GACAGTCCAC CGCCTCTGCC GGAGAAAAAG
    GGCAAACAGA TCCTGCAATA CATGCAGTTT GTGGAGGATT ACTCTGAGCC GCAGCCGTCC
    GTCTTCTACC AGCTGCCTCA GAGCGAGAGT ATTTACGAGC AGCGCAGGAA CAAGCGCTTT
    CAGGAGGTTT ACGGCCTCAA CGACTCCTAC AGCAGCTCCG ACTCCATTCC TGCTTCAGCT
    GCAGATCCGC ACGAACGGGT CCCACCACCA GCGCTGCCTC CAAAACTCAG ACAACTGGCC
    TCCAACACTA CTACTTTTCC TCCTTCATCC TCCTCTTCTG CTTTTTCAAC TCTTCCTCCT
    CCTGCTGGGC TTCCGGAGGA GGTGGAGCCT GCTCTTGGTC TCAGCCTATC AGTGTCTAGC
    TCTTTCCTCA GTGGCCACGC CTCTTTGACC ACTCCTGTGA GTTCCGAGCC GGTTGGCTTG
    ACCAATAGCG GCAGAGCTCT GGATGGTGGA GGTGTCTCAA ACGGGTCTTT GACCAGCTCT
    CTGCTCTCTC TGGCTGTCTG TCTTCCTTCT GAGTCGTCTC TCTCTGACTC GCTTCTTACC
    TCAGCGAGCG AAAGCATTGA TGAAAGTGGT GAAGGAGAAT ATGTAAACCT GTACTCGTCC
    ACACAGGCCA ACGGAGAAAA CACACACCGC ACTGAATCTT TATCATGTGA CGATGTGCTT
    CAAGACCACT CCCCTCACAT TCCCTCGTCC AATAGCAAGG AGGCTCTGAC TAAGGAGAGG
    CCGAAGGAAG CCAGTCACGG CCACATTGAT GATGTGGATG AATTATCCCT GATTGATCAT
    AATGACATCA TGAACCGCAT CACACTCAAA GCAGAGAATG ATGATGGTCC TGATGTGAGA
    GCTGGATCTG TAGACATACT GTTAGTTCAC GCTACAGAAA CAGACCGCAA AGATTTGGTG
    TTGTACTGCG AGGCGTTTCT CACAACCTAC AGAACCTTCA TAACACCAGA AGACCTCATT
    AAAAAGCTAC ACCACAGATA TACTCGCTTC TGCCACAGTC CAGACACCTT TAAGAAGCGT
    GTCAGTAAAA ACACCTTCTT TGTCCTGGTG AGAGTCGTGG ATGAACTTTG TCTGGTAGAG
    TTGACCGAGG ACATCCTGCG GCAGTTGATG GATCTGGTGT TTCGGCTGGT CTGTAACGGT
    GAACTGAGTC TGGCGAGAGT CCTGCGGAAA AATATCCTGG ATAAAGTGGA GCAGAAGAGA
    CTCCTGCAGC ACATGCACAC ACTCAGGCCT CTGGCGGCAC TCGGCGTCTC TGCCAGGCCA
    GGAACACTGC ATGATTTCCG CAGTCATGAG ATCGCCGATC AGCTAACGTT GCTGGACGCA
    GAACTCTTTT ACAAGATTGA GATTCCTGAA GTCCTGCTTT GGGCAAAGGA GCAGAACGAA
    GAGAAAAGTC CAAATCTGAC ACAGTTTACA GAGCACTTTA ACAACATGAG CTACTGGGTG
    CGCTCTATAA TCATCCAGCA GGAGAAAGCT CAGGACAGAG AGAAGCTACT GCTCAAATTC
    ATCAAAATCA TGAAGCACTT GCGTAAGCTG AATAACTTCA ACTCTTACCT GGCGATTCTC
    TCGGCTCTGG ACTCTGCACC GATCAGACGG CTGGAGTGGC AGAAACAGAC GTCTGAGGGT
    TTGGAGGAAT ACTGCACTCT CATCGACAGC TCTTCGTCAT TCAGAGCATA CAGAGCAGCG
    TTGGCGGAAG TTGAGCCTCC ATGTATACCT TACCTCGGTT TGATCCTGCA GGATCTGACG
    TTTGTTCATC TGGGAAACCC GGATTTTATA GAGGGGAAAG TGAATTTCTC CAAGCGCTGG
    CAGCAGTTCA ACATTCTGGA CAGCATGAGA CGCTTCCAGC AAGTACACTA CGACCTGAAG
    CGAAACGACG ACATCGTCTC GTTCTTCAAC GACTTTAGCG ATCACCTGGC GGAGGAGGCA
    TTATGGGAAC TCTCGCTCAA GATCAAACCC CGAAACATCT CGCGGCGCAG GACGGAGCGA
    GAGGAGAAGA CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021324455.1
    CDS302..3916
    Translation

    Target ORF information:

    RefSeq Version XM_021468780.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rap guanine nucleotide exchange factor (GEF) 1b (rapgef1b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468780.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGGAACA GCAGTCGGAG AACTCAGCCT CTTCTGGAGG ACACGGACTA CTTTAATCCT 
    GGAGAAGCAG ATACAGAATC CCAGAAGTCT CACCTGTCGT CCTTCACGAT GAAACTGATG
    AAGTTTCATT CCCCGAAGAT TAAACGGACC CCATCTAAGA AAGGCAAACA ACTCCAGCCT
    GAGCCTGCAG TGAAAACGCC AGAGAAGCCA GTCAACAAGA AGGTGAGTCG ACTGGAGGAA
    CAGGAGAAGG ATGTGGTCAG CGCTCTGAGA TACTTTAAGA CGATTGTGGA CAAGATGAAC
    GTTGACACCA AAGTTCTCCA GATGCTTCCT GGTTCGGCTA GTAAAGTTTT AGAGGCTATT
    TTGCCTCTGA TGCAGGTCGA GGCTCGGATA CAGCACAGCT CCGCCATGTC CTCCTGTCAC
    AACCGTGTTT ATCAGAGCTT GGCGACTCTG ATTCGCTGGT CCGACCAGGT GATGCTGGAG
    GGCATCGACC TCGACGACAA GGACACTGTG ACGACTGTTA CCACGGTGAT CAAAGCTGTG
    CTGGATGGTG TGAAGGAGCT TGTGAAACTC ACTATAGAGA AACATGACCA ACCGTCCCCA
    ACATCCCCCA GCAAACCAGC GCCTCCTGTG GCCAAAACAG AGAGTGGGTG TGAAAATCCT
    CTAACCGAAA GAGAGAAGGC CATCCTGAGT AAGACCACGC CCATTGTCAC GCCCACTGAC
    AGCTTGACTG ACATGTCAGA GGAGGAGGCT CCACCCAAAC CTCCTCTACC AGGACCCAAA
    CTAGCAGAGC ACAGGAACAG GATGCAGTTG TGCTCCGATC CTGTTACCGG GCAGCGGAGG
    GCTACGATGA TGGAAACTCC ACCGGCTCTG CCTCCGAAGA AGCGTCAGTC GGCCCCGTCA
    CCCACCCGTG TGGCAGTGGT GGCCCCGATG AGCCGGGGTC CGTGTAGCCT CAGCCTGCCT
    CCTGGAGCCC TCAGACAGCA GCAGGACTTT GATGTGGATC TCCTCCAGAG GCGTTTCTCA
    GGCGGCAGTC AGTCATATGA TGGAGAATCT CCTCGTCTTT CTCCCTGTAA CAGCATGGGA
    AAACTCAGCA AATCTGATGA GCAGCTTTCG TCTCTGGAGC GGGACAGTGG ACAGTGCTCT
    CGCAACACCA GCTGTGAAAC GCTTGAGGGA TACGATCCAG ATTACGACTT TTTAAATCAG
    GATCTGTCCG TCTCGGAACC GCTGCCGTTT CCCACCAACA CTGGCGGCAG TCTGAGCCCT
    CTTCCAGAGT GCAACACTGA GTCTCAATCT CTAAGCCCCG CCCACATCCC TGCACGCCCA
    CGCTTCAGCG CTCCAGTAAC CAATCAGGCC TCCCTAGACT ACTGGACTCC ACCCCTCACA
    CCTGGACAGG GTAATAGCGT GCTTCCCCCT GCCCTCCCAC AGAAGAAGCG CCGCTCTGCA
    CCAATCATAT CGGCGTATCC TCTGTACGAG CGCCTCCCGT CACACTACGA TAACCTATCA
    GAGGAGGAGC AGCCGACGCC GCCGTTTCCA CTGGTTACAC CGGGGTCGCC GCAGGTCAAC
    GGAGACGGTG TTTTACAGTA CACAGACGCT GACAGTCCAC CGCCTCTGCC GGAGAAAAAG
    GGCAAACAGA TCCTGCAATA CATGCAGTTT GTGGAGGATT ACTCTGAGCC GCAGCCGTCC
    GTCTTCTACC AGCTGCCTCA GAGCGAGAGT ATTTACGAGC AGCGCAGGAA CAAGCGCTTT
    CAGGAGGTTT ACGGCCTCAA CGACTCCTAC AGCAGCTCCG ACTCCATTCC TGCTTCAGCT
    GCAGATCCGC ACGAACGGGT CCCACCACCA GCGCTGCCTC CAAAACTCAG ACAACTGGCC
    TCCAACACTA CTACTTTTCC TCCTTCATCC TCCTCTTCTG CTTTTTCAAC TCTTCCTCCT
    CCTGCTGGGC TTCCGGAGGA GGTGGAGCCT GCTCTTGGTC TCAGCCTATC AGTGTCTAGC
    TCTTTCCTCA GTGGCCACGC CTCTTTGACC ACTCCTGTGA GTTCCGAGCC GGTTGGCTTG
    ACCAATAGCG GCAGAGCTCT GGATGGTGGA GGTGTCTCAA ACGGGTCTTT GACCAGCTCT
    CTGCTCTCTC TGGCTGTCTG TCTTCCTTCT GAGTCGTCTC TCTCTGACTC GCTTCTTACC
    TCAGCGAGCG AAAGCATTGA TGAAAGTGGT GAAGGAGAAT ATGTAAACCT GTACTCGTCC
    ACACAGGCCA ACGGAGAAAA CACACACCGC ACTGAATCTT TATCATGTGA CGATGTGCTT
    CAAGACCACT CCCCTCACAT TCCCTCGTCC AATAGCAAGG AGGCTCTGAC TAAGGAGAGG
    CCGAAGGAAG CCAGTCACGG CCACATTGAT GATGTGGATG AATTATCCCT GATTGATCAT
    AATGACATCA TGAACCGCAT CACACTCAAA GCAGAGAATG ATGATGGTCC TGATGTGAGA
    GCTGGATCTG TAGACATACT GTTAGTTCAC GCTACAGAAA CAGACCGCAA AGATTTGGTG
    TTGTACTGCG AGGCGTTTCT CACAACCTAC AGAACCTTCA TAACACCAGA AGACCTCATT
    AAAAAGCTAC ACCACAGATA TACTCGCTTC TGCCACAGTC CAGACACCTT TAAGAAGCGT
    GTCAGTAAAA ACACCTTCTT TGTCCTGGTG AGAGTCGTGG ATGAACTTTG TCTGGTAGAG
    TTGACCGAGG ACATCCTGCG GCAGTTGATG GATCTGGTGT TTCGGCTGGT CTGTAACGGT
    GAACTGAGTC TGGCGAGAGT CCTGCGGAAA AATATCCTGG ATAAAGTGGA GCAGAAGAGA
    CTCCTGCAGC ACATGCACAC ACTCAGGCCT CTGGCGGCAC TCGGCGTCTC TGCCAGGCCA
    GGAACACTGC ATGATTTCCG CAGTCATGAG ATCGCCGATC AGCTAACGTT GCTGGACGCA
    GAACTCTTTT ACAAGATTGA GATTCCTGAA GTCCTGCTTT GGGCAAAGGA GCAGAACGAA
    GAGAAAAGTC CAAATCTGAC ACAGTTTACA GAGCACTTTA ACAACATGAG CTACTGGGTG
    CGCTCTATAA TCATCCAGCA GGAGAAAGCT CAGGACAGAG AGAAGCTACT GCTCAAATTC
    ATCAAAATCA TGAAGCACTT GCGTAAGCTG AATAACTTCA ACTCTTACCT GGCGATTCTC
    TCGGCTCTGG ACTCTGCACC GATCAGACGG CTGGAGTGGC AGAAACAGAC GTCTGAGGGT
    TTGGAGGAAT ACTGCACTCT CATCGACAGC TCTTCGTCAT TCAGAGCATA CAGAGCAGCG
    TTGGCGGAAG TTGAGCCTCC ATGTATACCT TACCTCGGTT TGATCCTGCA GGATCTGACG
    TTTGTTCATC TGGGAAACCC GGATTTTATA GAGGGGAAAG TGAATTTCTC CAAGCGCTGG
    CAGCAGTTCA ACATTCTGGA CAGCATGAGA CGCTTCCAGC AAGTACACTA CGACCTGAAG
    CGAAACGACG ACATCGTCTC GTTCTTCAAC GACTTTAGCG ATCACCTGGC GGAGGAGGCA
    TTATGGGAAC TCTCGCTCAA GATCAAACCC CGAAACATCT CGCGGCGCAG GACGGAGCGA
    GAGGAGAAGA CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa150862
    Clone ID Related Accession (Same CDS sequence) XM_021468781.1
    Accession Version XM_021468781.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3612bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rap guanine nucleotide exchange factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGGAACA GCAGTCGGAG AACTCAGCCT CTTCTGGAGG ACACGGACTA CTTTAATCCT 
    GGAGAAGCAG ATACAGAATC CCAGAAGTCT CACCTGTCGT CCTTCACGAT GAAACTGATG
    AAGTTTCATT CCCCGAAGAT TAAACGGACC CCATCTAAGA AAGGCAAACA ACTCCAGCCT
    GAGCCTGCAG TGAAAACGCC AGAGAAGCCA GTCAACAAGA AGGTGAGTCG ACTGGAGGAA
    CAGGAGAAGG ATGTGGTCAG CGCTCTGAGA TACTTTAAGA CGATTGTGGA CAAGATGAAC
    GTTGACACCA AAGTTCTCCA GATGCTTCCT GGTTCGGCTA GTAAAGTTTT AGAGGCTATT
    TTGCCTCTGA TGCAGGTCGA GGCTCGGATA CAGCACAGCT CCGCCATGTC CTCCTGTCAC
    AACCGTGTTT ATCAGAGCTT GGCGACTCTG ATTCGCTGGT CCGACCAGGT GATGCTGGAG
    GGCATCGACC TCGACGACAA GGACACTGTG ACGACTGTTA CCACGGTGAT CAAAGCTGTG
    CTGGATGGTG TGAAGGAGCT TGTGAAACTC ACTATAGAGA AACATGACCA ACCGTCCCCA
    ACATCCCCCA GCAAACCAGC GCCTCCTGTG GCCAAAACAG AGAGTGGGTG TGAAAATCCT
    CTAACCGAAA GAGAGAAGGC CATCCTGAGT AAGACCACGC CCATTGTCAC GCCCACTGAC
    AGCTTGACTG ACATGTCAGA GGAGGAGGCT CCACCCAAAC CTCCTCTACC AGGACCCAAA
    CTAGCAGAGC ACAGGAACAG GATGCAGTTG TGCTCCGATC CTGTTACCGG GCAGCGGAGG
    GCTACGATGA TGGAAACTCC ACCGGCTCTG CCTCCGAAGA AGCGTCAGTC GGCCCCGTCA
    CCCACCCGTG TGGCAGTGGT GGCCCCGATG AGCCGGGGTC CGTGTAGCCT CAGCCTGCCT
    CCTGGAGCCC TCAGACAGCA GGACTTTGAT GTGGATCTCC TCCAGAGGCG TTTCTCAGGC
    GGCAGTCAGT CATATGATGG AGAATCTCCT CGTCTTTCTC CCTGTAACAG CATGGGAAAA
    CTCAGCAAAT CTGATGAGCA GCTTTCGTCT CTGGAGCGGG ACAGTGGACA GTGCTCTCGC
    AACACCAGCT GTGAAACGCT TGAGGGATAC GATCCAGATT ACGACTTTTT AAATCAGGAT
    CTGTCCGTCT CGGAACCGCT GCCGTTTCCC ACCAACACTG GCGGCAGTCT GAGCCCTCTT
    CCAGAGTGCA ACACTGAGTC TCAATCTCTA AGCCCCGCCC ACATCCCTGC ACGCCCACGC
    TTCAGCGCTC CAGTAACCAA TCAGGCCTCC CTAGACTACT GGACTCCACC CCTCACACCT
    GGACAGGGTA ATAGCGTGCT TCCCCCTGCC CTCCCACAGA AGAAGCGCCG CTCTGCACCA
    ATCATATCGG CGTATCCTCT GTACGAGCGC CTCCCGTCAC ACTACGATAA CCTATCAGAG
    GAGGAGCAGC CGACGCCGCC GTTTCCACTG GTTACACCGG GGTCGCCGCA GGTCAACGGA
    GACGGTGTTT TACAGTACAC AGACGCTGAC AGTCCACCGC CTCTGCCGGA GAAAAAGGGC
    AAACAGATCC TGCAATACAT GCAGTTTGTG GAGGATTACT CTGAGCCGCA GCCGTCCGTC
    TTCTACCAGC TGCCTCAGAG CGAGAGTATT TACGAGCAGC GCAGGAACAA GCGCTTTCAG
    GAGGTTTACG GCCTCAACGA CTCCTACAGC AGCTCCGACT CCATTCCTGC TTCAGCTGCA
    GATCCGCACG AACGGGTCCC ACCACCAGCG CTGCCTCCAA AACTCAGACA ACTGGCCTCC
    AACACTACTA CTTTTCCTCC TTCATCCTCC TCTTCTGCTT TTTCAACTCT TCCTCCTCCT
    GCTGGGCTTC CGGAGGAGGT GGAGCCTGCT CTTGGTCTCA GCCTATCAGT GTCTAGCTCT
    TTCCTCAGTG GCCACGCCTC TTTGACCACT CCTGTGAGTT CCGAGCCGGT TGGCTTGACC
    AATAGCGGCA GAGCTCTGGA TGGTGGAGGT GTCTCAAACG GGTCTTTGAC CAGCTCTCTG
    CTCTCTCTGG CTGTCTGTCT TCCTTCTGAG TCGTCTCTCT CTGACTCGCT TCTTACCTCA
    GCGAGCGAAA GCATTGATGA AAGTGGTGAA GGAGAATATG TAAACCTGTA CTCGTCCACA
    CAGGCCAACG GAGAAAACAC ACACCGCACT GAATCTTTAT CATGTGACGA TGTGCTTCAA
    GACCACTCCC CTCACATTCC CTCGTCCAAT AGCAAGGAGG CTCTGACTAA GGAGAGGCCG
    AAGGAAGCCA GTCACGGCCA CATTGATGAT GTGGATGAAT TATCCCTGAT TGATCATAAT
    GACATCATGA ACCGCATCAC ACTCAAAGCA GAGAATGATG ATGGTCCTGA TGTGAGAGCT
    GGATCTGTAG ACATACTGTT AGTTCACGCT ACAGAAACAG ACCGCAAAGA TTTGGTGTTG
    TACTGCGAGG CGTTTCTCAC AACCTACAGA ACCTTCATAA CACCAGAAGA CCTCATTAAA
    AAGCTACACC ACAGATATAC TCGCTTCTGC CACAGTCCAG ACACCTTTAA GAAGCGTGTC
    AGTAAAAACA CCTTCTTTGT CCTGGTGAGA GTCGTGGATG AACTTTGTCT GGTAGAGTTG
    ACCGAGGACA TCCTGCGGCA GTTGATGGAT CTGGTGTTTC GGCTGGTCTG TAACGGTGAA
    CTGAGTCTGG CGAGAGTCCT GCGGAAAAAT ATCCTGGATA AAGTGGAGCA GAAGAGACTC
    CTGCAGCACA TGCACACACT CAGGCCTCTG GCGGCACTCG GCGTCTCTGC CAGGCCAGGA
    ACACTGCATG ATTTCCGCAG TCATGAGATC GCCGATCAGC TAACGTTGCT GGACGCAGAA
    CTCTTTTACA AGATTGAGAT TCCTGAAGTC CTGCTTTGGG CAAAGGAGCA GAACGAAGAG
    AAAAGTCCAA ATCTGACACA GTTTACAGAG CACTTTAACA ACATGAGCTA CTGGGTGCGC
    TCTATAATCA TCCAGCAGGA GAAAGCTCAG GACAGAGAGA AGCTACTGCT CAAATTCATC
    AAAATCATGA AGCACTTGCG TAAGCTGAAT AACTTCAACT CTTACCTGGC GATTCTCTCG
    GCTCTGGACT CTGCACCGAT CAGACGGCTG GAGTGGCAGA AACAGACGTC TGAGGGTTTG
    GAGGAATACT GCACTCTCAT CGACAGCTCT TCGTCATTCA GAGCATACAG AGCAGCGTTG
    GCGGAAGTTG AGCCTCCATG TATACCTTAC CTCGGTTTGA TCCTGCAGGA TCTGACGTTT
    GTTCATCTGG GAAACCCGGA TTTTATAGAG GGGAAAGTGA ATTTCTCCAA GCGCTGGCAG
    CAGTTCAACA TTCTGGACAG CATGAGACGC TTCCAGCAAG TACACTACGA CCTGAAGCGA
    AACGACGACA TCGTCTCGTT CTTCAACGAC TTTAGCGATC ACCTGGCGGA GGAGGCATTA
    TGGGAACTCT CGCTCAAGAT CAAACCCCGA AACATCTCGC GGCGCAGGAC GGAGCGAGAG
    GAGAAGACCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021324456.1
    CDS302..3913
    Translation

    Target ORF information:

    RefSeq Version XM_021468781.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rap guanine nucleotide exchange factor (GEF) 1b (rapgef1b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468781.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGGAACA GCAGTCGGAG AACTCAGCCT CTTCTGGAGG ACACGGACTA CTTTAATCCT 
    GGAGAAGCAG ATACAGAATC CCAGAAGTCT CACCTGTCGT CCTTCACGAT GAAACTGATG
    AAGTTTCATT CCCCGAAGAT TAAACGGACC CCATCTAAGA AAGGCAAACA ACTCCAGCCT
    GAGCCTGCAG TGAAAACGCC AGAGAAGCCA GTCAACAAGA AGGTGAGTCG ACTGGAGGAA
    CAGGAGAAGG ATGTGGTCAG CGCTCTGAGA TACTTTAAGA CGATTGTGGA CAAGATGAAC
    GTTGACACCA AAGTTCTCCA GATGCTTCCT GGTTCGGCTA GTAAAGTTTT AGAGGCTATT
    TTGCCTCTGA TGCAGGTCGA GGCTCGGATA CAGCACAGCT CCGCCATGTC CTCCTGTCAC
    AACCGTGTTT ATCAGAGCTT GGCGACTCTG ATTCGCTGGT CCGACCAGGT GATGCTGGAG
    GGCATCGACC TCGACGACAA GGACACTGTG ACGACTGTTA CCACGGTGAT CAAAGCTGTG
    CTGGATGGTG TGAAGGAGCT TGTGAAACTC ACTATAGAGA AACATGACCA ACCGTCCCCA
    ACATCCCCCA GCAAACCAGC GCCTCCTGTG GCCAAAACAG AGAGTGGGTG TGAAAATCCT
    CTAACCGAAA GAGAGAAGGC CATCCTGAGT AAGACCACGC CCATTGTCAC GCCCACTGAC
    AGCTTGACTG ACATGTCAGA GGAGGAGGCT CCACCCAAAC CTCCTCTACC AGGACCCAAA
    CTAGCAGAGC ACAGGAACAG GATGCAGTTG TGCTCCGATC CTGTTACCGG GCAGCGGAGG
    GCTACGATGA TGGAAACTCC ACCGGCTCTG CCTCCGAAGA AGCGTCAGTC GGCCCCGTCA
    CCCACCCGTG TGGCAGTGGT GGCCCCGATG AGCCGGGGTC CGTGTAGCCT CAGCCTGCCT
    CCTGGAGCCC TCAGACAGCA GGACTTTGAT GTGGATCTCC TCCAGAGGCG TTTCTCAGGC
    GGCAGTCAGT CATATGATGG AGAATCTCCT CGTCTTTCTC CCTGTAACAG CATGGGAAAA
    CTCAGCAAAT CTGATGAGCA GCTTTCGTCT CTGGAGCGGG ACAGTGGACA GTGCTCTCGC
    AACACCAGCT GTGAAACGCT TGAGGGATAC GATCCAGATT ACGACTTTTT AAATCAGGAT
    CTGTCCGTCT CGGAACCGCT GCCGTTTCCC ACCAACACTG GCGGCAGTCT GAGCCCTCTT
    CCAGAGTGCA ACACTGAGTC TCAATCTCTA AGCCCCGCCC ACATCCCTGC ACGCCCACGC
    TTCAGCGCTC CAGTAACCAA TCAGGCCTCC CTAGACTACT GGACTCCACC CCTCACACCT
    GGACAGGGTA ATAGCGTGCT TCCCCCTGCC CTCCCACAGA AGAAGCGCCG CTCTGCACCA
    ATCATATCGG CGTATCCTCT GTACGAGCGC CTCCCGTCAC ACTACGATAA CCTATCAGAG
    GAGGAGCAGC CGACGCCGCC GTTTCCACTG GTTACACCGG GGTCGCCGCA GGTCAACGGA
    GACGGTGTTT TACAGTACAC AGACGCTGAC AGTCCACCGC CTCTGCCGGA GAAAAAGGGC
    AAACAGATCC TGCAATACAT GCAGTTTGTG GAGGATTACT CTGAGCCGCA GCCGTCCGTC
    TTCTACCAGC TGCCTCAGAG CGAGAGTATT TACGAGCAGC GCAGGAACAA GCGCTTTCAG
    GAGGTTTACG GCCTCAACGA CTCCTACAGC AGCTCCGACT CCATTCCTGC TTCAGCTGCA
    GATCCGCACG AACGGGTCCC ACCACCAGCG CTGCCTCCAA AACTCAGACA ACTGGCCTCC
    AACACTACTA CTTTTCCTCC TTCATCCTCC TCTTCTGCTT TTTCAACTCT TCCTCCTCCT
    GCTGGGCTTC CGGAGGAGGT GGAGCCTGCT CTTGGTCTCA GCCTATCAGT GTCTAGCTCT
    TTCCTCAGTG GCCACGCCTC TTTGACCACT CCTGTGAGTT CCGAGCCGGT TGGCTTGACC
    AATAGCGGCA GAGCTCTGGA TGGTGGAGGT GTCTCAAACG GGTCTTTGAC CAGCTCTCTG
    CTCTCTCTGG CTGTCTGTCT TCCTTCTGAG TCGTCTCTCT CTGACTCGCT TCTTACCTCA
    GCGAGCGAAA GCATTGATGA AAGTGGTGAA GGAGAATATG TAAACCTGTA CTCGTCCACA
    CAGGCCAACG GAGAAAACAC ACACCGCACT GAATCTTTAT CATGTGACGA TGTGCTTCAA
    GACCACTCCC CTCACATTCC CTCGTCCAAT AGCAAGGAGG CTCTGACTAA GGAGAGGCCG
    AAGGAAGCCA GTCACGGCCA CATTGATGAT GTGGATGAAT TATCCCTGAT TGATCATAAT
    GACATCATGA ACCGCATCAC ACTCAAAGCA GAGAATGATG ATGGTCCTGA TGTGAGAGCT
    GGATCTGTAG ACATACTGTT AGTTCACGCT ACAGAAACAG ACCGCAAAGA TTTGGTGTTG
    TACTGCGAGG CGTTTCTCAC AACCTACAGA ACCTTCATAA CACCAGAAGA CCTCATTAAA
    AAGCTACACC ACAGATATAC TCGCTTCTGC CACAGTCCAG ACACCTTTAA GAAGCGTGTC
    AGTAAAAACA CCTTCTTTGT CCTGGTGAGA GTCGTGGATG AACTTTGTCT GGTAGAGTTG
    ACCGAGGACA TCCTGCGGCA GTTGATGGAT CTGGTGTTTC GGCTGGTCTG TAACGGTGAA
    CTGAGTCTGG CGAGAGTCCT GCGGAAAAAT ATCCTGGATA AAGTGGAGCA GAAGAGACTC
    CTGCAGCACA TGCACACACT CAGGCCTCTG GCGGCACTCG GCGTCTCTGC CAGGCCAGGA
    ACACTGCATG ATTTCCGCAG TCATGAGATC GCCGATCAGC TAACGTTGCT GGACGCAGAA
    CTCTTTTACA AGATTGAGAT TCCTGAAGTC CTGCTTTGGG CAAAGGAGCA GAACGAAGAG
    AAAAGTCCAA ATCTGACACA GTTTACAGAG CACTTTAACA ACATGAGCTA CTGGGTGCGC
    TCTATAATCA TCCAGCAGGA GAAAGCTCAG GACAGAGAGA AGCTACTGCT CAAATTCATC
    AAAATCATGA AGCACTTGCG TAAGCTGAAT AACTTCAACT CTTACCTGGC GATTCTCTCG
    GCTCTGGACT CTGCACCGAT CAGACGGCTG GAGTGGCAGA AACAGACGTC TGAGGGTTTG
    GAGGAATACT GCACTCTCAT CGACAGCTCT TCGTCATTCA GAGCATACAG AGCAGCGTTG
    GCGGAAGTTG AGCCTCCATG TATACCTTAC CTCGGTTTGA TCCTGCAGGA TCTGACGTTT
    GTTCATCTGG GAAACCCGGA TTTTATAGAG GGGAAAGTGA ATTTCTCCAA GCGCTGGCAG
    CAGTTCAACA TTCTGGACAG CATGAGACGC TTCCAGCAAG TACACTACGA CCTGAAGCGA
    AACGACGACA TCGTCTCGTT CTTCAACGAC TTTAGCGATC ACCTGGCGGA GGAGGCATTA
    TGGGAACTCT CGCTCAAGAT CAAACCCCGA AACATCTCGC GGCGCAGGAC GGAGCGAGAG
    GAGAAGACCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa150863
    Clone ID Related Accession (Same CDS sequence) XM_021468782.1
    Accession Version XM_021468782.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3552bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rap guanine nucleotide exchange factor 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGGGAACA GCAGTCGGAG AACTCAGCCT CTTCTGGAGG ACACGGACTA CTTTAATCCT 
    GGAGAAGCAG ATACAGAATC CCAGAAGTCT CACCTGTCGT CCTTCACGAT GAAACTGATG
    AAGTTTCATT CCCCGAAGAT TAAACGGACC CCATCTAAGA AAGGCAAACA ACTCCAGCCT
    GAGCCTGCAG TGAAAACGCC AGAGAAGCCA GTCAACAAGA AGGTGAGTCG ACTGGAGGAA
    CAGGAGAAGG ATGTGGTCAG CGCTCTGAGA TACTTTAAGA CGATTGTGGA CAAGATGAAC
    GTTGACACCA AAGTTCTCCA GATGCTTCCT GGTTCGGCTA GTAAAGTTTT AGAGGCTATT
    TTGCCTCTGA TGCAGGTCGA GGCTCGGATA CAGCACAGCT CCGCCATGTC CTCCTGTCAC
    AACCGTGTTT ATCAGAGCTT GGCGACTCTG ATTCGCTGGT CCGACCAGGT GATGCTGGAG
    GGCATCGACC TCGACGACAA GGACACTGTG ACGACTGTTA CCACGGTGAT CAAAGCTGTG
    CTGGATGGTG TGAAGGAGCT TGTGAAACTC ACTATAGAGA AACATGACCA ACCGTCCCCA
    ACATCCCCCA GCAAACCAGC GCCTCCTGTG GCCAAAACAG AGAGTGGGTG TGAAAATCCT
    CTAACCGAAA GAGAGAAGGC CATCCTGAGT AAGACCACGC CCATTGTCAC GCCCACTGAC
    AGCTTGACTG ACATGTCAGA GGAGGAGGCT CCACCCAAAC CTCCTCTACC AGGACCCAAA
    CTAGCAGAGC ACAGTCCACC GGCTCTGCCT CCGAAGAAGC GTCAGTCGGC CCCGTCACCC
    ACCCGTGTGG CAGTGGTGGC CCCGATGAGC CGGGGTCCGT GTAGCCTCAG CCTGCCTCCT
    GGAGCCCTCA GACAGCAGCA GGACTTTGAT GTGGATCTCC TCCAGAGGCG TTTCTCAGGC
    GGCAGTCAGT CATATGATGG AGAATCTCCT CGTCTTTCTC CCTGTAACAG CATGGGAAAA
    CTCAGCAAAT CTGATGAGCA GCTTTCGTCT CTGGAGCGGG ACAGTGGACA GTGCTCTCGC
    AACACCAGCT GTGAAACGCT TGAGGGATAC GATCCAGATT ACGACTTTTT AAATCAGGAT
    CTGTCCGTCT CGGAACCGCT GCCGTTTCCC ACCAACACTG GCGGCAGTCT GAGCCCTCTT
    CCAGAGTGCA ACACTGAGTC TCAATCTCTA AGCCCCGCCC ACATCCCTGC ACGCCCACGC
    TTCAGCGCTC CAGTAACCAA TCAGGCCTCC CTAGACTACT GGACTCCACC CCTCACACCT
    GGACAGGGTA ATAGCGTGCT TCCCCCTGCC CTCCCACAGA AGAAGCGCCG CTCTGCACCA
    ATCATATCGG CGTATCCTCT GTACGAGCGC CTCCCGTCAC ACTACGATAA CCTATCAGAG
    GAGGAGCAGC CGACGCCGCC GTTTCCACTG GTTACACCGG GGTCGCCGCA GGTCAACGGA
    GACGGTGTTT TACAGTACAC AGACGCTGAC AGTCCACCGC CTCTGCCGGA GAAAAAGGGC
    AAACAGATCC TGCAATACAT GCAGTTTGTG GAGGATTACT CTGAGCCGCA GCCGTCCGTC
    TTCTACCAGC TGCCTCAGAG CGAGAGTATT TACGAGCAGC GCAGGAACAA GCGCTTTCAG
    GAGGTTTACG GCCTCAACGA CTCCTACAGC AGCTCCGACT CCATTCCTGC TTCAGCTGCA
    GATCCGCACG AACGGGTCCC ACCACCAGCG CTGCCTCCAA AACTCAGACA ACTGGCCTCC
    AACACTACTA CTTTTCCTCC TTCATCCTCC TCTTCTGCTT TTTCAACTCT TCCTCCTCCT
    GCTGGGCTTC CGGAGGAGGT GGAGCCTGCT CTTGGTCTCA GCCTATCAGT GTCTAGCTCT
    TTCCTCAGTG GCCACGCCTC TTTGACCACT CCTGTGAGTT CCGAGCCGGT TGGCTTGACC
    AATAGCGGCA GAGCTCTGGA TGGTGGAGGT GTCTCAAACG GGTCTTTGAC CAGCTCTCTG
    CTCTCTCTGG CTGTCTGTCT TCCTTCTGAG TCGTCTCTCT CTGACTCGCT TCTTACCTCA
    GCGAGCGAAA GCATTGATGA AAGTGGTGAA GGAGAATATG TAAACCTGTA CTCGTCCACA
    CAGGCCAACG GAGAAAACAC ACACCGCACT GAATCTTTAT CATGTGACGA TGTGCTTCAA
    GACCACTCCC CTCACATTCC CTCGTCCAAT AGCAAGGAGG CTCTGACTAA GGAGAGGCCG
    AAGGAAGCCA GTCACGGCCA CATTGATGAT GTGGATGAAT TATCCCTGAT TGATCATAAT
    GACATCATGA ACCGCATCAC ACTCAAAGCA GAGAATGATG ATGGTCCTGA TGTGAGAGCT
    GGATCTGTAG ACATACTGTT AGTTCACGCT ACAGAAACAG ACCGCAAAGA TTTGGTGTTG
    TACTGCGAGG CGTTTCTCAC AACCTACAGA ACCTTCATAA CACCAGAAGA CCTCATTAAA
    AAGCTACACC ACAGATATAC TCGCTTCTGC CACAGTCCAG ACACCTTTAA GAAGCGTGTC
    AGTAAAAACA CCTTCTTTGT CCTGGTGAGA GTCGTGGATG AACTTTGTCT GGTAGAGTTG
    ACCGAGGACA TCCTGCGGCA GTTGATGGAT CTGGTGTTTC GGCTGGTCTG TAACGGTGAA
    CTGAGTCTGG CGAGAGTCCT GCGGAAAAAT ATCCTGGATA AAGTGGAGCA GAAGAGACTC
    CTGCAGCACA TGCACACACT CAGGCCTCTG GCGGCACTCG GCGTCTCTGC CAGGCCAGGA
    ACACTGCATG ATTTCCGCAG TCATGAGATC GCCGATCAGC TAACGTTGCT GGACGCAGAA
    CTCTTTTACA AGATTGAGAT TCCTGAAGTC CTGCTTTGGG CAAAGGAGCA GAACGAAGAG
    AAAAGTCCAA ATCTGACACA GTTTACAGAG CACTTTAACA ACATGAGCTA CTGGGTGCGC
    TCTATAATCA TCCAGCAGGA GAAAGCTCAG GACAGAGAGA AGCTACTGCT CAAATTCATC
    AAAATCATGA AGCACTTGCG TAAGCTGAAT AACTTCAACT CTTACCTGGC GATTCTCTCG
    GCTCTGGACT CTGCACCGAT CAGACGGCTG GAGTGGCAGA AACAGACGTC TGAGGGTTTG
    GAGGAATACT GCACTCTCAT CGACAGCTCT TCGTCATTCA GAGCATACAG AGCAGCGTTG
    GCGGAAGTTG AGCCTCCATG TATACCTTAC CTCGGTTTGA TCCTGCAGGA TCTGACGTTT
    GTTCATCTGG GAAACCCGGA TTTTATAGAG GGGAAAGTGA ATTTCTCCAA GCGCTGGCAG
    CAGTTCAACA TTCTGGACAG CATGAGACGC TTCCAGCAAG TACACTACGA CCTGAAGCGA
    AACGACGACA TCGTCTCGTT CTTCAACGAC TTTAGCGATC ACCTGGCGGA GGAGGCATTA
    TGGGAACTCT CGCTCAAGAT CAAACCCCGA AACATCTCGC GGCGCAGGAC GGAGCGAGAG
    GAGAAGACCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021324457.1
    CDS300..3851
    Translation

    Target ORF information:

    RefSeq Version XM_021468782.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rap guanine nucleotide exchange factor (GEF) 1b (rapgef1b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468782.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGGGAACA GCAGTCGGAG AACTCAGCCT CTTCTGGAGG ACACGGACTA CTTTAATCCT 
    GGAGAAGCAG ATACAGAATC CCAGAAGTCT CACCTGTCGT CCTTCACGAT GAAACTGATG
    AAGTTTCATT CCCCGAAGAT TAAACGGACC CCATCTAAGA AAGGCAAACA ACTCCAGCCT
    GAGCCTGCAG TGAAAACGCC AGAGAAGCCA GTCAACAAGA AGGTGAGTCG ACTGGAGGAA
    CAGGAGAAGG ATGTGGTCAG CGCTCTGAGA TACTTTAAGA CGATTGTGGA CAAGATGAAC
    GTTGACACCA AAGTTCTCCA GATGCTTCCT GGTTCGGCTA GTAAAGTTTT AGAGGCTATT
    TTGCCTCTGA TGCAGGTCGA GGCTCGGATA CAGCACAGCT CCGCCATGTC CTCCTGTCAC
    AACCGTGTTT ATCAGAGCTT GGCGACTCTG ATTCGCTGGT CCGACCAGGT GATGCTGGAG
    GGCATCGACC TCGACGACAA GGACACTGTG ACGACTGTTA CCACGGTGAT CAAAGCTGTG
    CTGGATGGTG TGAAGGAGCT TGTGAAACTC ACTATAGAGA AACATGACCA ACCGTCCCCA
    ACATCCCCCA GCAAACCAGC GCCTCCTGTG GCCAAAACAG AGAGTGGGTG TGAAAATCCT
    CTAACCGAAA GAGAGAAGGC CATCCTGAGT AAGACCACGC CCATTGTCAC GCCCACTGAC
    AGCTTGACTG ACATGTCAGA GGAGGAGGCT CCACCCAAAC CTCCTCTACC AGGACCCAAA
    CTAGCAGAGC ACAGTCCACC GGCTCTGCCT CCGAAGAAGC GTCAGTCGGC CCCGTCACCC
    ACCCGTGTGG CAGTGGTGGC CCCGATGAGC CGGGGTCCGT GTAGCCTCAG CCTGCCTCCT
    GGAGCCCTCA GACAGCAGCA GGACTTTGAT GTGGATCTCC TCCAGAGGCG TTTCTCAGGC
    GGCAGTCAGT CATATGATGG AGAATCTCCT CGTCTTTCTC CCTGTAACAG CATGGGAAAA
    CTCAGCAAAT CTGATGAGCA GCTTTCGTCT CTGGAGCGGG ACAGTGGACA GTGCTCTCGC
    AACACCAGCT GTGAAACGCT TGAGGGATAC GATCCAGATT ACGACTTTTT AAATCAGGAT
    CTGTCCGTCT CGGAACCGCT GCCGTTTCCC ACCAACACTG GCGGCAGTCT GAGCCCTCTT
    CCAGAGTGCA ACACTGAGTC TCAATCTCTA AGCCCCGCCC ACATCCCTGC ACGCCCACGC
    TTCAGCGCTC CAGTAACCAA TCAGGCCTCC CTAGACTACT GGACTCCACC CCTCACACCT
    GGACAGGGTA ATAGCGTGCT TCCCCCTGCC CTCCCACAGA AGAAGCGCCG CTCTGCACCA
    ATCATATCGG CGTATCCTCT GTACGAGCGC CTCCCGTCAC ACTACGATAA CCTATCAGAG
    GAGGAGCAGC CGACGCCGCC GTTTCCACTG GTTACACCGG GGTCGCCGCA GGTCAACGGA
    GACGGTGTTT TACAGTACAC AGACGCTGAC AGTCCACCGC CTCTGCCGGA GAAAAAGGGC
    AAACAGATCC TGCAATACAT GCAGTTTGTG GAGGATTACT CTGAGCCGCA GCCGTCCGTC
    TTCTACCAGC TGCCTCAGAG CGAGAGTATT TACGAGCAGC GCAGGAACAA GCGCTTTCAG
    GAGGTTTACG GCCTCAACGA CTCCTACAGC AGCTCCGACT CCATTCCTGC TTCAGCTGCA
    GATCCGCACG AACGGGTCCC ACCACCAGCG CTGCCTCCAA AACTCAGACA ACTGGCCTCC
    AACACTACTA CTTTTCCTCC TTCATCCTCC TCTTCTGCTT TTTCAACTCT TCCTCCTCCT
    GCTGGGCTTC CGGAGGAGGT GGAGCCTGCT CTTGGTCTCA GCCTATCAGT GTCTAGCTCT
    TTCCTCAGTG GCCACGCCTC TTTGACCACT CCTGTGAGTT CCGAGCCGGT TGGCTTGACC
    AATAGCGGCA GAGCTCTGGA TGGTGGAGGT GTCTCAAACG GGTCTTTGAC CAGCTCTCTG
    CTCTCTCTGG CTGTCTGTCT TCCTTCTGAG TCGTCTCTCT CTGACTCGCT TCTTACCTCA
    GCGAGCGAAA GCATTGATGA AAGTGGTGAA GGAGAATATG TAAACCTGTA CTCGTCCACA
    CAGGCCAACG GAGAAAACAC ACACCGCACT GAATCTTTAT CATGTGACGA TGTGCTTCAA
    GACCACTCCC CTCACATTCC CTCGTCCAAT AGCAAGGAGG CTCTGACTAA GGAGAGGCCG
    AAGGAAGCCA GTCACGGCCA CATTGATGAT GTGGATGAAT TATCCCTGAT TGATCATAAT
    GACATCATGA ACCGCATCAC ACTCAAAGCA GAGAATGATG ATGGTCCTGA TGTGAGAGCT
    GGATCTGTAG ACATACTGTT AGTTCACGCT ACAGAAACAG ACCGCAAAGA TTTGGTGTTG
    TACTGCGAGG CGTTTCTCAC AACCTACAGA ACCTTCATAA CACCAGAAGA CCTCATTAAA
    AAGCTACACC ACAGATATAC TCGCTTCTGC CACAGTCCAG ACACCTTTAA GAAGCGTGTC
    AGTAAAAACA CCTTCTTTGT CCTGGTGAGA GTCGTGGATG AACTTTGTCT GGTAGAGTTG
    ACCGAGGACA TCCTGCGGCA GTTGATGGAT CTGGTGTTTC GGCTGGTCTG TAACGGTGAA
    CTGAGTCTGG CGAGAGTCCT GCGGAAAAAT ATCCTGGATA AAGTGGAGCA GAAGAGACTC
    CTGCAGCACA TGCACACACT CAGGCCTCTG GCGGCACTCG GCGTCTCTGC CAGGCCAGGA
    ACACTGCATG ATTTCCGCAG TCATGAGATC GCCGATCAGC TAACGTTGCT GGACGCAGAA
    CTCTTTTACA AGATTGAGAT TCCTGAAGTC CTGCTTTGGG CAAAGGAGCA GAACGAAGAG
    AAAAGTCCAA ATCTGACACA GTTTACAGAG CACTTTAACA ACATGAGCTA CTGGGTGCGC
    TCTATAATCA TCCAGCAGGA GAAAGCTCAG GACAGAGAGA AGCTACTGCT CAAATTCATC
    AAAATCATGA AGCACTTGCG TAAGCTGAAT AACTTCAACT CTTACCTGGC GATTCTCTCG
    GCTCTGGACT CTGCACCGAT CAGACGGCTG GAGTGGCAGA AACAGACGTC TGAGGGTTTG
    GAGGAATACT GCACTCTCAT CGACAGCTCT TCGTCATTCA GAGCATACAG AGCAGCGTTG
    GCGGAAGTTG AGCCTCCATG TATACCTTAC CTCGGTTTGA TCCTGCAGGA TCTGACGTTT
    GTTCATCTGG GAAACCCGGA TTTTATAGAG GGGAAAGTGA ATTTCTCCAA GCGCTGGCAG
    CAGTTCAACA TTCTGGACAG CATGAGACGC TTCCAGCAAG TACACTACGA CCTGAAGCGA
    AACGACGACA TCGTCTCGTT CTTCAACGAC TTTAGCGATC ACCTGGCGGA GGAGGCATTA
    TGGGAACTCT CGCTCAAGAT CAAACCCCGA AACATCTCGC GGCGCAGGAC GGAGCGAGAG
    GAGAAGACCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa150864
    Clone ID Related Accession (Same CDS sequence) XM_021468783.1
    Accession Version XM_021468783.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3468bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rap guanine nucleotide exchange factor 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGGGAACA GCAGTCGGAG AACTCAGCCT CTTCTGGAGG ACACGGACTA CTTTAATCCT 
    GGAGAAGCAG ATACAGAATC CCAGAAGTCT CACCTGTCGT CCTTCACGAT GAAACTGATG
    AAGTTTCATT CCCCGAAGAT TAAACGGACC CCATCTAAGA AAGGCAAACA ACTCCAGCCT
    GAGCCTGCAG TGAAAACGCC AGAGAAGCCA GTCAACAAGA AGGTGAGTCG ACTGGAGGAA
    CAGGAGAAGG ATGTGGTCAG CGCTCTGAGA TACTTTAAGA CGATTGTGGA CAAGATGAAC
    GTTGACACCA AAGTTCTCCA GATGCTTCCT GGTTCGGCTA GTAAAGTTTT AGAGGCTATT
    TTGCCTCTGA TGCAGGTCGA GGCTCGGATA CAGCACAGCT CCGCCATGTC CTCCTGTCAC
    AACCGTGTTT ATCAGAGCTT GGCGACTCTG ATTCGCTGGT CCGACCAGGT GATGCTGGAG
    GGCATCGACC TCGACGACAA GGACACTGTG ACGACTGTTA CCACGGTGAT CAAAGCTGTG
    CTGGATGGTG TGAAGGAGCT TGTGAAACTC ACTATAGAGA AACATGACCA ACCGTCCCCA
    ACATCCCCCA GCAAACCAGC GCCTCCTGTG GCCAAAACAG AGAGTGGGTG TGAAAATCCT
    CTAACCGAAA GAGAGAAGGC CATCCTGAGT AAGACCACGC CCATTGTCAC GCCCACTGAC
    AGCTTGACTG ACATGTCAGA GGAGGAGGCT CCACCCAAAC CTCCTCTACC AGGACCCAAA
    CTAGCAGAGC ACAGGAACAG GATGCAGTTG TGCTCCGATC CTGTTACCGG GCAGCGGAGG
    GCTACGATGA TGGAAACTCC ACCGGCTCTG CCTCCGAAGA AGCGTCAGTC GGCCCCGTCA
    CCCACCCGTG TGGCAGTGGT GGCCCCGATG AGCCGGGGTC CGTGTAGCCT CAGCCTGCCT
    CCTGGAGCCC TCAGACAGCA GCAGGACTTT GATGTGGATC TCCTCCAGAG GCGTTTCTCA
    GGCGGCAGTC AGTCATATGA TGGAGAATCT CCTCGTCTTT CTCCCTGTAA CAGCATGGGA
    AAACTCAGCA AATCTGATGA GCAGCTTTCG TCTCTGGAGC GGGACAGTGG ACAGTGCTCT
    CGCAACACCA GCTGTGAAAC GCTTGAGGGA TACGATCCAG ATTACGACTT TTTAAATCAG
    GATCTGTCCG TCTCGGAACC GCTGCCGTTT CCCACCAACA CTGGCGGCAG TCTGAGCCCT
    CTTCCAGAGT GCAACACTGA GTCTCAATCT CTAAGCCCCG CCCACATCCC TGCACGCCCA
    CGCTTCAGCG CTCCAGTAAC CAATCAGGCC TCCCTAGACT ACTGGACTCC ACCCCTCACA
    CCTGGACAGG GTAATAGCGT GCTTCCCCCT GCCCTCCCAC AGAAGAAGCG CCGCTCTGCA
    CCAATCATAT CGGCGTATCC TCTGTACGAG CGCCTCCCGT CACACTACGA TAACCTATCA
    GAGGAGGAGC AGCCGACGCC GCCGTTTCCA CTGGTTACAC CGGGGTCGCC GCAGGTCAAC
    GGAGACGGTG TTTTACAGTA CACAGACGCT GACAGTCCAC CGCCTCTGCC GGAGAAAAAG
    GGCAAACAGA TCCTGCAATA CATGCAGTTT GTGGAGGATT ACTCTGAGCC GCAGCCGTCC
    GTCTTCTACC AGCTGCCTCA GAGCGAGAGT ATTTACGAGC AGCGCAGGAA CAAGCGCTTT
    CAGGAGGTTT ACGGCCTCAA CGACTCCTAC AGCAGCTCCG ACTCCATTCC TGCTTCAGCT
    GCAGATCCGC ACGAACGGGT CCCACCACCA GCGCTGCCTC CAAAACTCAG ACAACTGGCC
    TCCAACACTA CTACTTTTCC TCCTTCATCC TCCTCTTCTG CTTTTTCAAC TCTTCCTCCT
    CCTGCTGGGC TTCCGGAGGA GGTGGAGCCT GCTCTTGGTC TCAGCCTATC AGTGTCTAGC
    TCTTTCCTCA GTGGCCACGC CTCTTTGACC ACTCCTGTGA GCGAAAGCAT TGATGAAAGT
    GGTGAAGGAG AATATGTAAA CCTGTACTCG TCCACACAGG CCAACGGAGA AAACACACAC
    CGCACTGAAT CTTTATCATG TGACGATGTG CTTCAAGACC ACTCCCCTCA CATTCCCTCG
    TCCAATAGCA AGGAGGCTCT GACTAAGGAG AGGCCGAAGG AAGCCAGTCA CGGCCACATT
    GATGATGTGG ATGAATTATC CCTGATTGAT CATAATGACA TCATGAACCG CATCACACTC
    AAAGCAGAGA ATGATGATGG TCCTGATGTG AGAGCTGGAT CTGTAGACAT ACTGTTAGTT
    CACGCTACAG AAACAGACCG CAAAGATTTG GTGTTGTACT GCGAGGCGTT TCTCACAACC
    TACAGAACCT TCATAACACC AGAAGACCTC ATTAAAAAGC TACACCACAG ATATACTCGC
    TTCTGCCACA GTCCAGACAC CTTTAAGAAG CGTGTCAGTA AAAACACCTT CTTTGTCCTG
    GTGAGAGTCG TGGATGAACT TTGTCTGGTA GAGTTGACCG AGGACATCCT GCGGCAGTTG
    ATGGATCTGG TGTTTCGGCT GGTCTGTAAC GGTGAACTGA GTCTGGCGAG AGTCCTGCGG
    AAAAATATCC TGGATAAAGT GGAGCAGAAG AGACTCCTGC AGCACATGCA CACACTCAGG
    CCTCTGGCGG CACTCGGCGT CTCTGCCAGG CCAGGAACAC TGCATGATTT CCGCAGTCAT
    GAGATCGCCG ATCAGCTAAC GTTGCTGGAC GCAGAACTCT TTTACAAGAT TGAGATTCCT
    GAAGTCCTGC TTTGGGCAAA GGAGCAGAAC GAAGAGAAAA GTCCAAATCT GACACAGTTT
    ACAGAGCACT TTAACAACAT GAGCTACTGG GTGCGCTCTA TAATCATCCA GCAGGAGAAA
    GCTCAGGACA GAGAGAAGCT ACTGCTCAAA TTCATCAAAA TCATGAAGCA CTTGCGTAAG
    CTGAATAACT TCAACTCTTA CCTGGCGATT CTCTCGGCTC TGGACTCTGC ACCGATCAGA
    CGGCTGGAGT GGCAGAAACA GACGTCTGAG GGTTTGGAGG AATACTGCAC TCTCATCGAC
    AGCTCTTCGT CATTCAGAGC ATACAGAGCA GCGTTGGCGG AAGTTGAGCC TCCATGTATA
    CCTTACCTCG GTTTGATCCT GCAGGATCTG ACGTTTGTTC ATCTGGGAAA CCCGGATTTT
    ATAGAGGGGA AAGTGAATTT CTCCAAGCGC TGGCAGCAGT TCAACATTCT GGACAGCATG
    AGACGCTTCC AGCAAGTACA CTACGACCTG AAGCGAAACG ACGACATCGT CTCGTTCTTC
    AACGACTTTA GCGATCACCT GGCGGAGGAG GCATTATGGG AACTCTCGCT CAAGATCAAA
    CCCCGAAACA TCTCGCGGCG CAGGACGGAG CGAGAGGAGA AGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021324458.1
    CDS301..3768
    Translation

    Target ORF information:

    RefSeq Version XM_021468783.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rap guanine nucleotide exchange factor (GEF) 1b (rapgef1b), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468783.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGGGAACA GCAGTCGGAG AACTCAGCCT CTTCTGGAGG ACACGGACTA CTTTAATCCT 
    GGAGAAGCAG ATACAGAATC CCAGAAGTCT CACCTGTCGT CCTTCACGAT GAAACTGATG
    AAGTTTCATT CCCCGAAGAT TAAACGGACC CCATCTAAGA AAGGCAAACA ACTCCAGCCT
    GAGCCTGCAG TGAAAACGCC AGAGAAGCCA GTCAACAAGA AGGTGAGTCG ACTGGAGGAA
    CAGGAGAAGG ATGTGGTCAG CGCTCTGAGA TACTTTAAGA CGATTGTGGA CAAGATGAAC
    GTTGACACCA AAGTTCTCCA GATGCTTCCT GGTTCGGCTA GTAAAGTTTT AGAGGCTATT
    TTGCCTCTGA TGCAGGTCGA GGCTCGGATA CAGCACAGCT CCGCCATGTC CTCCTGTCAC
    AACCGTGTTT ATCAGAGCTT GGCGACTCTG ATTCGCTGGT CCGACCAGGT GATGCTGGAG
    GGCATCGACC TCGACGACAA GGACACTGTG ACGACTGTTA CCACGGTGAT CAAAGCTGTG
    CTGGATGGTG TGAAGGAGCT TGTGAAACTC ACTATAGAGA AACATGACCA ACCGTCCCCA
    ACATCCCCCA GCAAACCAGC GCCTCCTGTG GCCAAAACAG AGAGTGGGTG TGAAAATCCT
    CTAACCGAAA GAGAGAAGGC CATCCTGAGT AAGACCACGC CCATTGTCAC GCCCACTGAC
    AGCTTGACTG ACATGTCAGA GGAGGAGGCT CCACCCAAAC CTCCTCTACC AGGACCCAAA
    CTAGCAGAGC ACAGGAACAG GATGCAGTTG TGCTCCGATC CTGTTACCGG GCAGCGGAGG
    GCTACGATGA TGGAAACTCC ACCGGCTCTG CCTCCGAAGA AGCGTCAGTC GGCCCCGTCA
    CCCACCCGTG TGGCAGTGGT GGCCCCGATG AGCCGGGGTC CGTGTAGCCT CAGCCTGCCT
    CCTGGAGCCC TCAGACAGCA GCAGGACTTT GATGTGGATC TCCTCCAGAG GCGTTTCTCA
    GGCGGCAGTC AGTCATATGA TGGAGAATCT CCTCGTCTTT CTCCCTGTAA CAGCATGGGA
    AAACTCAGCA AATCTGATGA GCAGCTTTCG TCTCTGGAGC GGGACAGTGG ACAGTGCTCT
    CGCAACACCA GCTGTGAAAC GCTTGAGGGA TACGATCCAG ATTACGACTT TTTAAATCAG
    GATCTGTCCG TCTCGGAACC GCTGCCGTTT CCCACCAACA CTGGCGGCAG TCTGAGCCCT
    CTTCCAGAGT GCAACACTGA GTCTCAATCT CTAAGCCCCG CCCACATCCC TGCACGCCCA
    CGCTTCAGCG CTCCAGTAAC CAATCAGGCC TCCCTAGACT ACTGGACTCC ACCCCTCACA
    CCTGGACAGG GTAATAGCGT GCTTCCCCCT GCCCTCCCAC AGAAGAAGCG CCGCTCTGCA
    CCAATCATAT CGGCGTATCC TCTGTACGAG CGCCTCCCGT CACACTACGA TAACCTATCA
    GAGGAGGAGC AGCCGACGCC GCCGTTTCCA CTGGTTACAC CGGGGTCGCC GCAGGTCAAC
    GGAGACGGTG TTTTACAGTA CACAGACGCT GACAGTCCAC CGCCTCTGCC GGAGAAAAAG
    GGCAAACAGA TCCTGCAATA CATGCAGTTT GTGGAGGATT ACTCTGAGCC GCAGCCGTCC
    GTCTTCTACC AGCTGCCTCA GAGCGAGAGT ATTTACGAGC AGCGCAGGAA CAAGCGCTTT
    CAGGAGGTTT ACGGCCTCAA CGACTCCTAC AGCAGCTCCG ACTCCATTCC TGCTTCAGCT
    GCAGATCCGC ACGAACGGGT CCCACCACCA GCGCTGCCTC CAAAACTCAG ACAACTGGCC
    TCCAACACTA CTACTTTTCC TCCTTCATCC TCCTCTTCTG CTTTTTCAAC TCTTCCTCCT
    CCTGCTGGGC TTCCGGAGGA GGTGGAGCCT GCTCTTGGTC TCAGCCTATC AGTGTCTAGC
    TCTTTCCTCA GTGGCCACGC CTCTTTGACC ACTCCTGTGA GCGAAAGCAT TGATGAAAGT
    GGTGAAGGAG AATATGTAAA CCTGTACTCG TCCACACAGG CCAACGGAGA AAACACACAC
    CGCACTGAAT CTTTATCATG TGACGATGTG CTTCAAGACC ACTCCCCTCA CATTCCCTCG
    TCCAATAGCA AGGAGGCTCT GACTAAGGAG AGGCCGAAGG AAGCCAGTCA CGGCCACATT
    GATGATGTGG ATGAATTATC CCTGATTGAT CATAATGACA TCATGAACCG CATCACACTC
    AAAGCAGAGA ATGATGATGG TCCTGATGTG AGAGCTGGAT CTGTAGACAT ACTGTTAGTT
    CACGCTACAG AAACAGACCG CAAAGATTTG GTGTTGTACT GCGAGGCGTT TCTCACAACC
    TACAGAACCT TCATAACACC AGAAGACCTC ATTAAAAAGC TACACCACAG ATATACTCGC
    TTCTGCCACA GTCCAGACAC CTTTAAGAAG CGTGTCAGTA AAAACACCTT CTTTGTCCTG
    GTGAGAGTCG TGGATGAACT TTGTCTGGTA GAGTTGACCG AGGACATCCT GCGGCAGTTG
    ATGGATCTGG TGTTTCGGCT GGTCTGTAAC GGTGAACTGA GTCTGGCGAG AGTCCTGCGG
    AAAAATATCC TGGATAAAGT GGAGCAGAAG AGACTCCTGC AGCACATGCA CACACTCAGG
    CCTCTGGCGG CACTCGGCGT CTCTGCCAGG CCAGGAACAC TGCATGATTT CCGCAGTCAT
    GAGATCGCCG ATCAGCTAAC GTTGCTGGAC GCAGAACTCT TTTACAAGAT TGAGATTCCT
    GAAGTCCTGC TTTGGGCAAA GGAGCAGAAC GAAGAGAAAA GTCCAAATCT GACACAGTTT
    ACAGAGCACT TTAACAACAT GAGCTACTGG GTGCGCTCTA TAATCATCCA GCAGGAGAAA
    GCTCAGGACA GAGAGAAGCT ACTGCTCAAA TTCATCAAAA TCATGAAGCA CTTGCGTAAG
    CTGAATAACT TCAACTCTTA CCTGGCGATT CTCTCGGCTC TGGACTCTGC ACCGATCAGA
    CGGCTGGAGT GGCAGAAACA GACGTCTGAG GGTTTGGAGG AATACTGCAC TCTCATCGAC
    AGCTCTTCGT CATTCAGAGC ATACAGAGCA GCGTTGGCGG AAGTTGAGCC TCCATGTATA
    CCTTACCTCG GTTTGATCCT GCAGGATCTG ACGTTTGTTC ATCTGGGAAA CCCGGATTTT
    ATAGAGGGGA AAGTGAATTT CTCCAAGCGC TGGCAGCAGT TCAACATTCT GGACAGCATG
    AGACGCTTCC AGCAAGTACA CTACGACCTG AAGCGAAACG ACGACATCGT CTCGTTCTTC
    AACGACTTTA GCGATCACCT GGCGGAGGAG GCATTATGGG AACTCTCGCT CAAGATCAAA
    CCCCGAAACA TCTCGCGGCG CAGGACGGAG CGAGAGGAGA AGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa150865
    Clone ID Related Accession (Same CDS sequence) XM_021468784.1
    Accession Version XM_021468784.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3306bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rap guanine nucleotide exchange factor 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGGGAACA GCAGTCGGAG AACTCAGCCT CTTCTGGAGG ACACGGACTA CTTTAATCCT 
    GGAGAAGCAG ATACAGAATC CCAGAAGTCT CACCTGTCGT CCTTCACGAT GAAACTGATG
    AAGTTTCATT CCCCGAAGAT TAAACGGACC CCATCTAAGA AAGGCAAACA ACTCCAGCCT
    GAGCCTGCAG TGAAAACGCC AGAGAAGCCA GTCAACAAGA AGGTGAGTCG ACTGGAGGAA
    CAGGAGAAGG ATGTGGTCAG CGCTCTGAGA TACTTTAAGA CGATTGTGGA CAAGATGAAC
    GTTGACACCA AAGTTCTCCA GATGCTTCCT GGTTCGGCTA GTAAAGTTTT AGAGGCTATT
    TTGCCTCTGA TGCAGGTCGA GGCTCGGATA CAGCACAGCT CCGCCATGTC CTCCTGTCAC
    AACCGTGTTT ATCAGAGCTT GGCGACTCTG ATTCGCTGGT CCGACCAGGT GATGCTGGAG
    GGCATCGACC TCGACGACAA GGACACTGTG ACGACTGTTA CCACGGTGAT CAAAGCTGTG
    CTGGATGGTG TGAAGGAGCT TGTGAAACTC ACTATAGAGA AACATGACCA ACCGTCCCCA
    ACATCCCCCA GCAAACCAGC GCCTCCTGTG GCCAAAACAG AGAGTGGGTG TGAAAATCCT
    CTAACCGAAA GAGAGAAGGC CATCCTGAGT AAGACCACGC CCATTGTCAC GCCCACTGAC
    AGCTTGACTG ACATGTCAGA GGAGGAGGCT CCACCCAAAC CTCCTCTACC AGGACCCAAA
    CTAGCAGAGC ACAGGAACAG GATGCAGTTG TGCTCCGATC CTGTTACCGG GCAGCGGAGG
    GCTACGATGA TGGAAACTCC ACCGGCTCTG CCTCCGAAGA AGCGTCAGTC GGCCCCGTCA
    CCCACCCGTG TGGCAGTGGT GGCCCCGATG AGCCGGGGTC CGTGTAGCCT CAGCCTGCCT
    CCTGGAGCCC TCAGACAGCA GCAGGACTTT GATGTGGATC TCCTCCAGAG GCGTTTCTCA
    GGCGGCAGTC AGTCATATGA TGGAGAATCT CCTCGTCTTT CTCCCTGTAA CAGCATGGGA
    AAACTCAGCA AATCTGATGA GCAGCTTTCG TCTCTGGAGC GGGACAGTGG ACAGTGCTCT
    CGCAACACCA GCTGTGAAAC GCTTGAGGGA TACGATCCAG ATTACGACTT TTTAAATCAG
    GATCTGTCCG TCTCGGAACC GCTGCCGTTT CCCACCAACA CTGGCGGCAG TCTGAGCCCT
    CTTCCAGAGT GCAACACTGA GTCTCAATCT CTAAGCCCCG CCCACATCCC TGCACGCCCA
    CGCTTCAGCG CTCCAGTAAC CAATCAGGCC TCCCTAGACT ACTGGACTCC ACCCCTCACA
    CCTGGACAGG GTAATAGCGT GCTTCCCCCT GCCCTCCCAC AGAAGAAGCG CCGCTCTGCA
    CCAATCATAT CGGCGTATCC TCTGTACGAG CGCCTCCCGT CACACTACGA TAACCTATCA
    GAGGAGGAGC AGCCGACGCC GCCGTTTCCA CTGGTTACAC CGGGGTCGCC GCAGGTCAAC
    GGAGACGGTG TTTTACAGTA CACAGACGCT GACAGTCCAC CGCCTCTGCC GGAGAAAAAG
    GGCAAACAGA TCCTGCAATA CATGCAGTTT GTGGAGGATT ACTCTGAGCC GCAGCCGTCC
    GTCTTCTACC AGCTGCCTCA GAGCGAGAGT ATTTACGAGC AGCGCAGGAA CAAGCGCTTT
    CAGGAGGTTT ACGGCCTCAA CGACTCCTAC AGCAGCTCCG ACTCCATTCC TGCTTCAGCT
    GCAGATCCGC ACGAACGGGT CCCACCACCA GCGCTGCCTC CAAAACTCAG ACAACTGAGC
    GAAAGCATTG ATGAAAGTGG TGAAGGAGAA TATGTAAACC TGTACTCGTC CACACAGGCC
    AACGGAGAAA ACACACACCG CACTGAATCT TTATCATGTG ACGATGTGCT TCAAGACCAC
    TCCCCTCACA TTCCCTCGTC CAATAGCAAG GAGGCTCTGA CTAAGGAGAG GCCGAAGGAA
    GCCAGTCACG GCCACATTGA TGATGTGGAT GAATTATCCC TGATTGATCA TAATGACATC
    ATGAACCGCA TCACACTCAA AGCAGAGAAT GATGATGGTC CTGATGTGAG AGCTGGATCT
    GTAGACATAC TGTTAGTTCA CGCTACAGAA ACAGACCGCA AAGATTTGGT GTTGTACTGC
    GAGGCGTTTC TCACAACCTA CAGAACCTTC ATAACACCAG AAGACCTCAT TAAAAAGCTA
    CACCACAGAT ATACTCGCTT CTGCCACAGT CCAGACACCT TTAAGAAGCG TGTCAGTAAA
    AACACCTTCT TTGTCCTGGT GAGAGTCGTG GATGAACTTT GTCTGGTAGA GTTGACCGAG
    GACATCCTGC GGCAGTTGAT GGATCTGGTG TTTCGGCTGG TCTGTAACGG TGAACTGAGT
    CTGGCGAGAG TCCTGCGGAA AAATATCCTG GATAAAGTGG AGCAGAAGAG ACTCCTGCAG
    CACATGCACA CACTCAGGCC TCTGGCGGCA CTCGGCGTCT CTGCCAGGCC AGGAACACTG
    CATGATTTCC GCAGTCATGA GATCGCCGAT CAGCTAACGT TGCTGGACGC AGAACTCTTT
    TACAAGATTG AGATTCCTGA AGTCCTGCTT TGGGCAAAGG AGCAGAACGA AGAGAAAAGT
    CCAAATCTGA CACAGTTTAC AGAGCACTTT AACAACATGA GCTACTGGGT GCGCTCTATA
    ATCATCCAGC AGGAGAAAGC TCAGGACAGA GAGAAGCTAC TGCTCAAATT CATCAAAATC
    ATGAAGCACT TGCGTAAGCT GAATAACTTC AACTCTTACC TGGCGATTCT CTCGGCTCTG
    GACTCTGCAC CGATCAGACG GCTGGAGTGG CAGAAACAGA CGTCTGAGGG TTTGGAGGAA
    TACTGCACTC TCATCGACAG CTCTTCGTCA TTCAGAGCAT ACAGAGCAGC GTTGGCGGAA
    GTTGAGCCTC CATGTATACC TTACCTCGGT TTGATCCTGC AGGATCTGAC GTTTGTTCAT
    CTGGGAAACC CGGATTTTAT AGAGGGGAAA GTGAATTTCT CCAAGCGCTG GCAGCAGTTC
    AACATTCTGG ACAGCATGAG ACGCTTCCAG CAAGTACACT ACGACCTGAA GCGAAACGAC
    GACATCGTCT CGTTCTTCAA CGACTTTAGC GATCACCTGG CGGAGGAGGC ATTATGGGAA
    CTCTCGCTCA AGATCAAACC CCGAAACATC TCGCGGCGCA GGACGGAGCG AGAGGAGAAG
    ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021324459.1
    CDS299..3604
    Translation

    Target ORF information:

    RefSeq Version XM_021468784.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rap guanine nucleotide exchange factor (GEF) 1b (rapgef1b), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468784.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGGGAACA GCAGTCGGAG AACTCAGCCT CTTCTGGAGG ACACGGACTA CTTTAATCCT 
    GGAGAAGCAG ATACAGAATC CCAGAAGTCT CACCTGTCGT CCTTCACGAT GAAACTGATG
    AAGTTTCATT CCCCGAAGAT TAAACGGACC CCATCTAAGA AAGGCAAACA ACTCCAGCCT
    GAGCCTGCAG TGAAAACGCC AGAGAAGCCA GTCAACAAGA AGGTGAGTCG ACTGGAGGAA
    CAGGAGAAGG ATGTGGTCAG CGCTCTGAGA TACTTTAAGA CGATTGTGGA CAAGATGAAC
    GTTGACACCA AAGTTCTCCA GATGCTTCCT GGTTCGGCTA GTAAAGTTTT AGAGGCTATT
    TTGCCTCTGA TGCAGGTCGA GGCTCGGATA CAGCACAGCT CCGCCATGTC CTCCTGTCAC
    AACCGTGTTT ATCAGAGCTT GGCGACTCTG ATTCGCTGGT CCGACCAGGT GATGCTGGAG
    GGCATCGACC TCGACGACAA GGACACTGTG ACGACTGTTA CCACGGTGAT CAAAGCTGTG
    CTGGATGGTG TGAAGGAGCT TGTGAAACTC ACTATAGAGA AACATGACCA ACCGTCCCCA
    ACATCCCCCA GCAAACCAGC GCCTCCTGTG GCCAAAACAG AGAGTGGGTG TGAAAATCCT
    CTAACCGAAA GAGAGAAGGC CATCCTGAGT AAGACCACGC CCATTGTCAC GCCCACTGAC
    AGCTTGACTG ACATGTCAGA GGAGGAGGCT CCACCCAAAC CTCCTCTACC AGGACCCAAA
    CTAGCAGAGC ACAGGAACAG GATGCAGTTG TGCTCCGATC CTGTTACCGG GCAGCGGAGG
    GCTACGATGA TGGAAACTCC ACCGGCTCTG CCTCCGAAGA AGCGTCAGTC GGCCCCGTCA
    CCCACCCGTG TGGCAGTGGT GGCCCCGATG AGCCGGGGTC CGTGTAGCCT CAGCCTGCCT
    CCTGGAGCCC TCAGACAGCA GCAGGACTTT GATGTGGATC TCCTCCAGAG GCGTTTCTCA
    GGCGGCAGTC AGTCATATGA TGGAGAATCT CCTCGTCTTT CTCCCTGTAA CAGCATGGGA
    AAACTCAGCA AATCTGATGA GCAGCTTTCG TCTCTGGAGC GGGACAGTGG ACAGTGCTCT
    CGCAACACCA GCTGTGAAAC GCTTGAGGGA TACGATCCAG ATTACGACTT TTTAAATCAG
    GATCTGTCCG TCTCGGAACC GCTGCCGTTT CCCACCAACA CTGGCGGCAG TCTGAGCCCT
    CTTCCAGAGT GCAACACTGA GTCTCAATCT CTAAGCCCCG CCCACATCCC TGCACGCCCA
    CGCTTCAGCG CTCCAGTAAC CAATCAGGCC TCCCTAGACT ACTGGACTCC ACCCCTCACA
    CCTGGACAGG GTAATAGCGT GCTTCCCCCT GCCCTCCCAC AGAAGAAGCG CCGCTCTGCA
    CCAATCATAT CGGCGTATCC TCTGTACGAG CGCCTCCCGT CACACTACGA TAACCTATCA
    GAGGAGGAGC AGCCGACGCC GCCGTTTCCA CTGGTTACAC CGGGGTCGCC GCAGGTCAAC
    GGAGACGGTG TTTTACAGTA CACAGACGCT GACAGTCCAC CGCCTCTGCC GGAGAAAAAG
    GGCAAACAGA TCCTGCAATA CATGCAGTTT GTGGAGGATT ACTCTGAGCC GCAGCCGTCC
    GTCTTCTACC AGCTGCCTCA GAGCGAGAGT ATTTACGAGC AGCGCAGGAA CAAGCGCTTT
    CAGGAGGTTT ACGGCCTCAA CGACTCCTAC AGCAGCTCCG ACTCCATTCC TGCTTCAGCT
    GCAGATCCGC ACGAACGGGT CCCACCACCA GCGCTGCCTC CAAAACTCAG ACAACTGAGC
    GAAAGCATTG ATGAAAGTGG TGAAGGAGAA TATGTAAACC TGTACTCGTC CACACAGGCC
    AACGGAGAAA ACACACACCG CACTGAATCT TTATCATGTG ACGATGTGCT TCAAGACCAC
    TCCCCTCACA TTCCCTCGTC CAATAGCAAG GAGGCTCTGA CTAAGGAGAG GCCGAAGGAA
    GCCAGTCACG GCCACATTGA TGATGTGGAT GAATTATCCC TGATTGATCA TAATGACATC
    ATGAACCGCA TCACACTCAA AGCAGAGAAT GATGATGGTC CTGATGTGAG AGCTGGATCT
    GTAGACATAC TGTTAGTTCA CGCTACAGAA ACAGACCGCA AAGATTTGGT GTTGTACTGC
    GAGGCGTTTC TCACAACCTA CAGAACCTTC ATAACACCAG AAGACCTCAT TAAAAAGCTA
    CACCACAGAT ATACTCGCTT CTGCCACAGT CCAGACACCT TTAAGAAGCG TGTCAGTAAA
    AACACCTTCT TTGTCCTGGT GAGAGTCGTG GATGAACTTT GTCTGGTAGA GTTGACCGAG
    GACATCCTGC GGCAGTTGAT GGATCTGGTG TTTCGGCTGG TCTGTAACGG TGAACTGAGT
    CTGGCGAGAG TCCTGCGGAA AAATATCCTG GATAAAGTGG AGCAGAAGAG ACTCCTGCAG
    CACATGCACA CACTCAGGCC TCTGGCGGCA CTCGGCGTCT CTGCCAGGCC AGGAACACTG
    CATGATTTCC GCAGTCATGA GATCGCCGAT CAGCTAACGT TGCTGGACGC AGAACTCTTT
    TACAAGATTG AGATTCCTGA AGTCCTGCTT TGGGCAAAGG AGCAGAACGA AGAGAAAAGT
    CCAAATCTGA CACAGTTTAC AGAGCACTTT AACAACATGA GCTACTGGGT GCGCTCTATA
    ATCATCCAGC AGGAGAAAGC TCAGGACAGA GAGAAGCTAC TGCTCAAATT CATCAAAATC
    ATGAAGCACT TGCGTAAGCT GAATAACTTC AACTCTTACC TGGCGATTCT CTCGGCTCTG
    GACTCTGCAC CGATCAGACG GCTGGAGTGG CAGAAACAGA CGTCTGAGGG TTTGGAGGAA
    TACTGCACTC TCATCGACAG CTCTTCGTCA TTCAGAGCAT ACAGAGCAGC GTTGGCGGAA
    GTTGAGCCTC CATGTATACC TTACCTCGGT TTGATCCTGC AGGATCTGAC GTTTGTTCAT
    CTGGGAAACC CGGATTTTAT AGAGGGGAAA GTGAATTTCT CCAAGCGCTG GCAGCAGTTC
    AACATTCTGG ACAGCATGAG ACGCTTCCAG CAAGTACACT ACGACCTGAA GCGAAACGAC
    GACATCGTCT CGTTCTTCAA CGACTTTAGC GATCACCTGG CGGAGGAGGC ATTATGGGAA
    CTCTCGCTCA AGATCAAACC CCGAAACATC TCGCGGCGCA GGACGGAGCG AGAGGAGAAG
    ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa56768
    Clone ID Related Accession (Same CDS sequence) XM_017352761.1
    Accession Version XM_017352761.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3564bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rap guanine nucleotide exchange factor 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGTCGGGGA AAATAGAAAG CAAGCACGAA TCCCAGAAGT CTCACCTGTC GTCCTTCACG 
    ATGAAACTGA TGAAGTTTCA TTCCCCGAAG ATTAAACGGA CCCCATCTAA GAAAGGCAAA
    CAACTCCAGC CTGAGCCTGC AGTGAAAACG CCAGAGAAGC CAGTCAACAA GAAGGTGAGT
    CGACTGGAGG AACAGGAGAA GGATGTGGTC AGCGCTCTGA GATACTTTAA GACGATTGTG
    GACAAGATGA ACGTTGACAC CAAAGTTCTC CAGATGCTTC CTGGTTCGGC TAGTAAAGTT
    TTAGAGGCTA TTTTGCCTCT GATGCAGGTC GAGGCTCGGA TACAGCACAG CTCCGCCATG
    TCCTCCTGTC ACAACCGTGT TTATCAGAGC TTGGCGACTC TGATTCGCTG GTCCGACCAG
    GTGATGCTGG AGGGCATCGA CCTCGACGAC AAGGACACTG TGACGACTGT TACCACGGTG
    ATCAAAGCTG TGCTGGATGG TGTGAAGGAG CTTGTGAAAC TCACTATAGA GAAACATGAC
    CAACCGTCCC CAACATCCCC CAGCAAACCA GCGCCTCCTG TGGCCAAAAC AGAGAGTGGG
    TGTGAAAATC CTCTAACCGA AAGAGAGAAG GCCATCCTGA GTAAGACCAC GCCCATTGTC
    ACGCCCACTG ACAGCTTGAC TGACATGTCA GAGGAGGAGG CTCCACCCAA ACCTCCTCTA
    CCAGGACCCA AACTAGCAGA GCACAGGAAC AGGATGCAGT TGTGCTCCGA TCCTGTTACC
    GGGCAGCGGA GGGCTACGAT GATGGAAACT CCACCGGCTC TGCCTCCGAA GAAGCGTCAG
    TCGGCCCCGT CACCCACCCG TGTGGCAGTG GTGGCCCCGA TGAGCCGGGG TCCGTGTAGC
    CTCAGCCTGC CTCCTGGAGC CCTCAGACAG CAGGACTTTG ATGTGGATCT CCTCCAGAGG
    CGTTTCTCAG GCGGCAGTCA GTCATATGAT GGAGAATCTC CTCGTCTTTC TCCCTGTAAC
    AGCATGGGAA AACTCAGCAA ATCTGATGAG CAGCTTTCGT CTCTGGAGCG GGACAGTGGA
    CAGTGCTCTC GCAACACCAG CTGTGAAACG CTTGAGGGAT ACGATCCAGA TTACGACTTT
    TTAAATCAGG ATCTGTCCGT CTCGGAACCG CTGCCGTTTC CCACCAACAC TGGCGGCAGT
    CTGAGCCCTC TTCCAGAGTG CAACACTGAG TCTCAATCTC TAAGCCCCGC CCACATCCCT
    GCACGCCCAC GCTTCAGCGC TCCAGTAACC AATCAGGCCT CCCTAGACTA CTGGACTCCA
    CCCCTCACAC CTGGACAGGG TAATAGCGTG CTTCCCCCTG CCCTCCCACA GAAGAAGCGC
    CGCTCTGCAC CAATCATATC GGCGTATCCT CTGTACGAGC GCCTCCCGTC ACACTACGAT
    AACCTATCAG AGGAGGAGCA GCCGACGCCG CCGTTTCCAC TGGTTACACC GGGGTCGCCG
    CAGGTCAACG GAGACGGTGT TTTACAGTAC ACAGACGCTG ACAGTCCACC GCCTCTGCCG
    GAGAAAAAGG GCAAACAGAT CCTGCAATAC ATGCAGTTTG TGGAGGATTA CTCTGAGCCG
    CAGCCGTCCG TCTTCTACCA GCTGCCTCAG AGCGAGAGTA TTTACGAGCA GCGCAGGAAC
    AAGCGCTTTC AGGAGGTTTA CGGCCTCAAC GACTCCTACA GCAGCTCCGA CTCCATTCCT
    GCTTCAGCTG CAGATCCGCA CGAACGGGTC CCACCACCAG CGCTGCCTCC AAAACTCAGA
    CAACTGGCCT CCAACACTAC TACTTTTCCT CCTTCATCCT CCTCTTCTGC TTTTTCAACT
    CTTCCTCCTC CTGCTGGGCT TCCGGAGGAG GTGGAGCCTG CTCTTGGTCT CAGCCTATCA
    GTGTCTAGCT CTTTCCTCAG TGGCCACGCC TCTTTGACCA CTCCTGTGAG TTCCGAGCCG
    GTTGGCTTGA CCAATAGCGG CAGAGCTCTG GATGGTGGAG GTGTCTCAAA CGGGTCTTTG
    ACCAGCTCTC TGCTCTCTCT GGCTGTCTGT CTTCCTTCTG AGTCGTCTCT CTCTGACTCG
    CTTCTTACCT CAGCGAGCGA AAGCATTGAT GAAAGTGGTG AAGGAGAATA TGTAAACCTG
    TACTCGTCCA CACAGGCCAA CGGAGAAAAC ACACACCGCA CTGAATCTTT ATCATGTGAC
    GATGTGCTTC AAGACCACTC CCCTCACATT CCCTCGTCCA ATAGCAAGGA GGCTCTGACT
    AAGGAGAGGC CGAAGGAAGC CAGTCACGGC CACATTGATG ATGTGGATGA ATTATCCCTG
    ATTGATCATA ATGACATCAT GAACCGCATC ACACTCAAAG CAGAGAATGA TGATGGTCCT
    GATGTGAGAG CTGGATCTGT AGACATACTG TTAGTTCACG CTACAGAAAC AGACCGCAAA
    GATTTGGTGT TGTACTGCGA GGCGTTTCTC ACAACCTACA GAACCTTCAT AACACCAGAA
    GACCTCATTA AAAAGCTACA CCACAGATAT ACTCGCTTCT GCCACAGTCC AGACACCTTT
    AAGAAGCGTG TCAGTAAAAA CACCTTCTTT GTCCTGGTGA GAGTCGTGGA TGAACTTTGT
    CTGGTAGAGT TGACCGAGGA CATCCTGCGG CAGTTGATGG ATCTGGTGTT TCGGCTGGTC
    TGTAACGGTG AACTGAGTCT GGCGAGAGTC CTGCGGAAAA ATATCCTGGA TAAAGTGGAG
    CAGAAGAGAC TCCTGCAGCA CATGCACACA CTCAGGCCTC TGGCGGCACT CGGCGTCTCT
    GCCAGGCCAG GAACACTGCA TGATTTCCGC AGTCATGAGA TCGCCGATCA GCTAACGTTG
    CTGGACGCAG AACTCTTTTA CAAGATTGAG ATTCCTGAAG TCCTGCTTTG GGCAAAGGAG
    CAGAACGAAG AGAAAAGTCC AAATCTGACA CAGTTTACAG AGCACTTTAA CAACATGAGC
    TACTGGGTGC GCTCTATAAT CATCCAGCAG GAGAAAGCTC AGGACAGAGA GAAGCTACTG
    CTCAAATTCA TCAAAATCAT GAAGCACTTG CGTAAGCTGA ATAACTTCAA CTCTTACCTG
    GCGATTCTCT CGGCTCTGGA CTCTGCACCG ATCAGACGGC TGGAGTGGCA GAAACAGACG
    TCTGAGGGTT TGGAGGAATA CTGCACTCTC ATCGACAGCT CTTCGTCATT CAGAGCATAC
    AGAGCAGCGT TGGCGGAAGT TGAGCCTCCA TGTATACCTT ACCTCGGTTT GATCCTGCAG
    GATCTGACGT TTGTTCATCT GGGAAACCCG GATTTTATAG AGGGGAAAGT GAATTTCTCC
    AAGCGCTGGC AGCAGTTCAA CATTCTGGAC AGCATGAGAC GCTTCCAGCA AGTACACTAC
    GACCTGAAGC GAAACGACGA CATCGTCTCG TTCTTCAACG ACTTTAGCGA TCACCTGGCG
    GAGGAGGCAT TATGGGAACT CTCGCTCAAG ATCAAACCCC GAAACATCTC GCGGCGCAGG
    ACGGAGCGAG AGGAGAAGAC CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017208250.1
    CDS293..3856
    Translation

    Target ORF information:

    RefSeq Version XM_017352761.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Rap guanine nucleotide exchange factor (GEF) 1b (rapgef1b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017352761.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGTCGGGGA AAATAGAAAG CAAGCACGAA TCCCAGAAGT CTCACCTGTC GTCCTTCACG 
    ATGAAACTGA TGAAGTTTCA TTCCCCGAAG ATTAAACGGA CCCCATCTAA GAAAGGCAAA
    CAACTCCAGC CTGAGCCTGC AGTGAAAACG CCAGAGAAGC CAGTCAACAA GAAGGTGAGT
    CGACTGGAGG AACAGGAGAA GGATGTGGTC AGCGCTCTGA GATACTTTAA GACGATTGTG
    GACAAGATGA ACGTTGACAC CAAAGTTCTC CAGATGCTTC CTGGTTCGGC TAGTAAAGTT
    TTAGAGGCTA TTTTGCCTCT GATGCAGGTC GAGGCTCGGA TACAGCACAG CTCCGCCATG
    TCCTCCTGTC ACAACCGTGT TTATCAGAGC TTGGCGACTC TGATTCGCTG GTCCGACCAG
    GTGATGCTGG AGGGCATCGA CCTCGACGAC AAGGACACTG TGACGACTGT TACCACGGTG
    ATCAAAGCTG TGCTGGATGG TGTGAAGGAG CTTGTGAAAC TCACTATAGA GAAACATGAC
    CAACCGTCCC CAACATCCCC CAGCAAACCA GCGCCTCCTG TGGCCAAAAC AGAGAGTGGG
    TGTGAAAATC CTCTAACCGA AAGAGAGAAG GCCATCCTGA GTAAGACCAC GCCCATTGTC
    ACGCCCACTG ACAGCTTGAC TGACATGTCA GAGGAGGAGG CTCCACCCAA ACCTCCTCTA
    CCAGGACCCA AACTAGCAGA GCACAGGAAC AGGATGCAGT TGTGCTCCGA TCCTGTTACC
    GGGCAGCGGA GGGCTACGAT GATGGAAACT CCACCGGCTC TGCCTCCGAA GAAGCGTCAG
    TCGGCCCCGT CACCCACCCG TGTGGCAGTG GTGGCCCCGA TGAGCCGGGG TCCGTGTAGC
    CTCAGCCTGC CTCCTGGAGC CCTCAGACAG CAGGACTTTG ATGTGGATCT CCTCCAGAGG
    CGTTTCTCAG GCGGCAGTCA GTCATATGAT GGAGAATCTC CTCGTCTTTC TCCCTGTAAC
    AGCATGGGAA AACTCAGCAA ATCTGATGAG CAGCTTTCGT CTCTGGAGCG GGACAGTGGA
    CAGTGCTCTC GCAACACCAG CTGTGAAACG CTTGAGGGAT ACGATCCAGA TTACGACTTT
    TTAAATCAGG ATCTGTCCGT CTCGGAACCG CTGCCGTTTC CCACCAACAC TGGCGGCAGT
    CTGAGCCCTC TTCCAGAGTG CAACACTGAG TCTCAATCTC TAAGCCCCGC CCACATCCCT
    GCACGCCCAC GCTTCAGCGC TCCAGTAACC AATCAGGCCT CCCTAGACTA CTGGACTCCA
    CCCCTCACAC CTGGACAGGG TAATAGCGTG CTTCCCCCTG CCCTCCCACA GAAGAAGCGC
    CGCTCTGCAC CAATCATATC GGCGTATCCT CTGTACGAGC GCCTCCCGTC ACACTACGAT
    AACCTATCAG AGGAGGAGCA GCCGACGCCG CCGTTTCCAC TGGTTACACC GGGGTCGCCG
    CAGGTCAACG GAGACGGTGT TTTACAGTAC ACAGACGCTG ACAGTCCACC GCCTCTGCCG
    GAGAAAAAGG GCAAACAGAT CCTGCAATAC ATGCAGTTTG TGGAGGATTA CTCTGAGCCG
    CAGCCGTCCG TCTTCTACCA GCTGCCTCAG AGCGAGAGTA TTTACGAGCA GCGCAGGAAC
    AAGCGCTTTC AGGAGGTTTA CGGCCTCAAC GACTCCTACA GCAGCTCCGA CTCCATTCCT
    GCTTCAGCTG CAGATCCGCA CGAACGGGTC CCACCACCAG CGCTGCCTCC AAAACTCAGA
    CAACTGGCCT CCAACACTAC TACTTTTCCT CCTTCATCCT CCTCTTCTGC TTTTTCAACT
    CTTCCTCCTC CTGCTGGGCT TCCGGAGGAG GTGGAGCCTG CTCTTGGTCT CAGCCTATCA
    GTGTCTAGCT CTTTCCTCAG TGGCCACGCC TCTTTGACCA CTCCTGTGAG TTCCGAGCCG
    GTTGGCTTGA CCAATAGCGG CAGAGCTCTG GATGGTGGAG GTGTCTCAAA CGGGTCTTTG
    ACCAGCTCTC TGCTCTCTCT GGCTGTCTGT CTTCCTTCTG AGTCGTCTCT CTCTGACTCG
    CTTCTTACCT CAGCGAGCGA AAGCATTGAT GAAAGTGGTG AAGGAGAATA TGTAAACCTG
    TACTCGTCCA CACAGGCCAA CGGAGAAAAC ACACACCGCA CTGAATCTTT ATCATGTGAC
    GATGTGCTTC AAGACCACTC CCCTCACATT CCCTCGTCCA ATAGCAAGGA GGCTCTGACT
    AAGGAGAGGC CGAAGGAAGC CAGTCACGGC CACATTGATG ATGTGGATGA ATTATCCCTG
    ATTGATCATA ATGACATCAT GAACCGCATC ACACTCAAAG CAGAGAATGA TGATGGTCCT
    GATGTGAGAG CTGGATCTGT AGACATACTG TTAGTTCACG CTACAGAAAC AGACCGCAAA
    GATTTGGTGT TGTACTGCGA GGCGTTTCTC ACAACCTACA GAACCTTCAT AACACCAGAA
    GACCTCATTA AAAAGCTACA CCACAGATAT ACTCGCTTCT GCCACAGTCC AGACACCTTT
    AAGAAGCGTG TCAGTAAAAA CACCTTCTTT GTCCTGGTGA GAGTCGTGGA TGAACTTTGT
    CTGGTAGAGT TGACCGAGGA CATCCTGCGG CAGTTGATGG ATCTGGTGTT TCGGCTGGTC
    TGTAACGGTG AACTGAGTCT GGCGAGAGTC CTGCGGAAAA ATATCCTGGA TAAAGTGGAG
    CAGAAGAGAC TCCTGCAGCA CATGCACACA CTCAGGCCTC TGGCGGCACT CGGCGTCTCT
    GCCAGGCCAG GAACACTGCA TGATTTCCGC AGTCATGAGA TCGCCGATCA GCTAACGTTG
    CTGGACGCAG AACTCTTTTA CAAGATTGAG ATTCCTGAAG TCCTGCTTTG GGCAAAGGAG
    CAGAACGAAG AGAAAAGTCC AAATCTGACA CAGTTTACAG AGCACTTTAA CAACATGAGC
    TACTGGGTGC GCTCTATAAT CATCCAGCAG GAGAAAGCTC AGGACAGAGA GAAGCTACTG
    CTCAAATTCA TCAAAATCAT GAAGCACTTG CGTAAGCTGA ATAACTTCAA CTCTTACCTG
    GCGATTCTCT CGGCTCTGGA CTCTGCACCG ATCAGACGGC TGGAGTGGCA GAAACAGACG
    TCTGAGGGTT TGGAGGAATA CTGCACTCTC ATCGACAGCT CTTCGTCATT CAGAGCATAC
    AGAGCAGCGT TGGCGGAAGT TGAGCCTCCA TGTATACCTT ACCTCGGTTT GATCCTGCAG
    GATCTGACGT TTGTTCATCT GGGAAACCCG GATTTTATAG AGGGGAAAGT GAATTTCTCC
    AAGCGCTGGC AGCAGTTCAA CATTCTGGAC AGCATGAGAC GCTTCCAGCA AGTACACTAC
    GACCTGAAGC GAAACGACGA CATCGTCTCG TTCTTCAACG ACTTTAGCGA TCACCTGGCG
    GAGGAGGCAT TATGGGAACT CTCGCTCAAG ATCAAACCCC GAAACATCTC GCGGCGCAGG
    ACGGAGCGAG AGGAGAAGAC CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa40639
    Clone ID Related Accession (Same CDS sequence) NM_001328346.1
    Accession Version NM_001328346.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3567bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-11-23
    Organism Danio rerio(zebrafish)
    Product rap guanine nucleotide exchange factor 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FP085431.9, CU570789.6 and CU571253.8. On Jun 14, 2016 this sequence version replaced XM_009295117.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA3505392, SAMEA4476744 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGTCGGGGA AAATAGAAAG CAAGCACGAA TCCCAGAAGT CTCACCTGTC GTCCTTCACG 
    ATGAAACTGA TGAAGTTTCA TTCCCCGAAG ATTAAACGGA CCCCATCTAA GAAAGGCAAA
    CAACTCCAGC CTGAGCCTGC AGTGAAAACG CCAGAGAAGC CAGTCAACAA GAAGGTGAGT
    CGACTGGAGG AACAGGAGAA GGATGTGGTC AGCGCTCTGA GATACTTTAA GACGATTGTG
    GACAAGATGA ACGTTGACAC CAAAGTTCTC CAGATGCTTC CTGGTTCGGC TAGTAAAGTT
    TTAGAGGCTA TTTTGCCTCT GATGCAGGTC GAGGCTCGGA TACAGCACAG CTCCGCCATG
    TCCTCCTGTC ACAACCGTGT TTATCAGAGC TTGGCGACTC TGATTCGCTG GTCCGACCAG
    GTGATGCTGG AGGGCATCGA CCTCGACGAC AAGGACACTG TGACGACTGT TACCACGGTG
    ATCAAAGCTG TGCTGGATGG TGTGAAGGAG CTTGTGAAAC TCACTATAGA GAAACATGAC
    CAACCGTCCC CAACATCCCC CAGCAAACCA GCGCCTCCTG TGGCCAAAAC AGAGAGTGGG
    TGTGAAAATC CTCTAACCGA AAGAGAGAAG GCCATCCTGA GTAAGACCAC GCCCATTGTC
    ACGCCCACTG ACAGCTTGAC TGACATGTCA GAGGAGGAGG CTCCACCCAA ACCTCCTCTA
    CCAGGACCCA AACTAGCAGA GCACAGGAAC AGGATGCAGT TGTGCTCCGA TCCTGTTACC
    GGGCAGCGGA GGGCTACGAT GATGGAAACT CCACCGGCTC TGCCTCCGAA GAAGCGTCAG
    TCGGCCCCGT CACCCACCCG TGTGGCAGTG GTGGCCCCGA TGAGCCGGGG TCCGTGTAGC
    CTCAGCCTGC CTCCTGGAGC CCTCAGACAG CAGCAGGACT TTGATGTGGA TCTCCTCCAG
    AGGCGTTTCT CAGGCGGCAG TCAGTCATAT GATGGAGAAT CTCCTCGTCT TTCTCCCTGT
    AACAGCATGG GAAAACTCAG CAAATCTGAT GAGCAGCTTT CGTCTCTGGA GCGGGACAGT
    GGACAGTGCT CTCGCAACAC CAGCTGTGAA ACGCTTGAGG GATACGATCC AGATTACGAC
    TTTTTAAATC AGGATCTGTC CGTCTCGGAA CCGCTGCCGT TTCCCACCAA CACTGGCGGC
    AGTCTGAGCC CTCTTCCAGA GTGCAACACT GAGTCTCAAT CTCTAAGCCC CGCCCACATC
    CCTGCACGCC CACGCTTCAG CGCTCCAGTA ACCAATCAGG CCTCCCTAGA CTACTGGACT
    CCACCCCTCA CACCTGGACA GGGTAATAGC GTGCTTCCCC CTGCCCTCCC ACAGAAGAAG
    CGCCGCTCTG CACCAATCAT ATCGGCGTAT CCTCTGTACG AGCGCCTCCC GTCACACTAC
    GATAACCTAT CAGAGGAGGA GCAGCCGACG CCGCCGTTTC CACTGGTTAC ACCGGGGTCG
    CCGCAGGTCA ACGGAGACGG TGTTTTACAG TACACAGACG CTGACAGTCC ACCGCCTCTG
    CCGGAGAAAA AGGGCAAACA GATCCTGCAA TACATGCAGT TTGTGGAGGA TTACTCTGAG
    CCGCAGCCGT CCGTCTTCTA CCAGCTGCCT CAGAGCGAGA GTATTTACGA GCAGCGCAGG
    AACAAGCGCT TTCAGGAGGT TTACGGCCTC AACGACTCCT ACAGCAGCTC CGACTCCATT
    CCTGCTTCAG CTGCAGATCC GCACGAACGG GTCCCACCAC CAGCGCTGCC TCCAAAACTC
    AGACAACTGG CCTCCAACAC TACTACTTTT CCTCCTTCAT CCTCCTCTTC TGCTTTTTCA
    ACTCTTCCTC CTCCTGCTGG GCTTCCGGAG GAGGTGGAGC CTGCTCTTGG TCTCAGCCTA
    TCAGTGTCTA GCTCTTTCCT CAGTGGCCAC GCCTCTTTGA CCACTCCTGT GAGTTCCGAG
    CCGGTTGGCT TGACCAATAG CGGCAGAGCT CTGGATGGTG GAGGTGTCTC AAACGGGTCT
    TTGACCAGCT CTCTGCTCTC TCTGGCTGTC TGTCTTCCTT CTGAGTCGTC TCTCTCTGAC
    TCGCTTCTTA CCTCAGCGAG CGAAAGCATT GATGAAAGTG GTGAAGGAGA ATATGTAAAC
    CTGTACTCGT CCACACAGGC CAACGGAGAA AACACACACC GCACTGAATC TTTATCATGT
    GACGATGTGC TTCAAGACCA CTCCCCTCAC ATTCCCTCGT CCAATAGCAA GGAGGCTCTG
    ACTAAGGAGA GGCCGAAGGA AGCCAGTCAC GGCCACATTG ATGATGTGGA TGAATTATCC
    CTGATTGATC ATAATGACAT CATGAACCGC ATCACACTCA AAGCAGAGAA TGATGATGGT
    CCTGATGTGA GAGCTGGATC TGTAGACATA CTGTTAGTTC ACGCTACAGA AACAGACCGC
    AAAGATTTGG TGTTGTACTG CGAGGCGTTT CTCACAACCT ACAGAACCTT CATAACACCA
    GAAGACCTCA TTAAAAAGCT ACACCACAGA TATACTCGCT TCTGCCACAG TCCAGACACC
    TTTAAGAAGC GTGTCAGTAA AAACACCTTC TTTGTCCTGG TGAGAGTCGT GGATGAACTT
    TGTCTGGTAG AGTTGACCGA GGACATCCTG CGGCAGTTGA TGGATCTGGT GTTTCGGCTG
    GTCTGTAACG GTGAACTGAG TCTGGCGAGA GTCCTGCGGA AAAATATCCT GGATAAAGTG
    GAGCAGAAGA GACTCCTGCA GCACATGCAC ACACTCAGGC CTCTGGCGGC ACTCGGCGTC
    TCTGCCAGGC CAGGAACACT GCATGATTTC CGCAGTCATG AGATCGCCGA TCAGCTAACG
    TTGCTGGACG CAGAACTCTT TTACAAGATT GAGATTCCTG AAGTCCTGCT TTGGGCAAAG
    GAGCAGAACG AAGAGAAAAG TCCAAATCTG ACACAGTTTA CAGAGCACTT TAACAACATG
    AGCTACTGGG TGCGCTCTAT AATCATCCAG CAGGAGAAAG CTCAGGACAG AGAGAAGCTA
    CTGCTCAAAT TCATCAAAAT CATGAAGCAC TTGCGTAAGC TGAATAACTT CAACTCTTAC
    CTGGCGATTC TCTCGGCTCT GGACTCTGCA CCGATCAGAC GGCTGGAGTG GCAGAAACAG
    ACGTCTGAGG GTTTGGAGGA ATACTGCACT CTCATCGACA GCTCTTCGTC ATTCAGAGCA
    TACAGAGCAG CGTTGGCGGA AGTTGAGCCT CCATGTATAC CTTACCTCGG TTTGATCCTG
    CAGGATCTGA CGTTTGTTCA TCTGGGAAAC CCGGATTTTA TAGAGGGGAA AGTGAATTTC
    TCCAAGCGCT GGCAGCAGTT CAACATTCTG GACAGCATGA GACGCTTCCA GCAAGTACAC
    TACGACCTGA AGCGAAACGA CGACATCGTC TCGTTCTTCA ACGACTTTAG CGATCACCTG
    GCGGAGGAGG CATTATGGGA ACTCTCGCTC AAGATCAAAC CCCGAAACAT CTCGCGGCGC
    AGGACGGAGC GAGAGGAGAA GACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001315275.1
    CDS227..3793
    Translation

    Target ORF information:

    RefSeq Version NM_001328346.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rap guanine nucleotide exchange factor (GEF) 1b (rapgef1b), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001328346.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGTCGGGGA AAATAGAAAG CAAGCACGAA TCCCAGAAGT CTCACCTGTC GTCCTTCACG 
    ATGAAACTGA TGAAGTTTCA TTCCCCGAAG ATTAAACGGA CCCCATCTAA GAAAGGCAAA
    CAACTCCAGC CTGAGCCTGC AGTGAAAACG CCAGAGAAGC CAGTCAACAA GAAGGTGAGT
    CGACTGGAGG AACAGGAGAA GGATGTGGTC AGCGCTCTGA GATACTTTAA GACGATTGTG
    GACAAGATGA ACGTTGACAC CAAAGTTCTC CAGATGCTTC CTGGTTCGGC TAGTAAAGTT
    TTAGAGGCTA TTTTGCCTCT GATGCAGGTC GAGGCTCGGA TACAGCACAG CTCCGCCATG
    TCCTCCTGTC ACAACCGTGT TTATCAGAGC TTGGCGACTC TGATTCGCTG GTCCGACCAG
    GTGATGCTGG AGGGCATCGA CCTCGACGAC AAGGACACTG TGACGACTGT TACCACGGTG
    ATCAAAGCTG TGCTGGATGG TGTGAAGGAG CTTGTGAAAC TCACTATAGA GAAACATGAC
    CAACCGTCCC CAACATCCCC CAGCAAACCA GCGCCTCCTG TGGCCAAAAC AGAGAGTGGG
    TGTGAAAATC CTCTAACCGA AAGAGAGAAG GCCATCCTGA GTAAGACCAC GCCCATTGTC
    ACGCCCACTG ACAGCTTGAC TGACATGTCA GAGGAGGAGG CTCCACCCAA ACCTCCTCTA
    CCAGGACCCA AACTAGCAGA GCACAGGAAC AGGATGCAGT TGTGCTCCGA TCCTGTTACC
    GGGCAGCGGA GGGCTACGAT GATGGAAACT CCACCGGCTC TGCCTCCGAA GAAGCGTCAG
    TCGGCCCCGT CACCCACCCG TGTGGCAGTG GTGGCCCCGA TGAGCCGGGG TCCGTGTAGC
    CTCAGCCTGC CTCCTGGAGC CCTCAGACAG CAGCAGGACT TTGATGTGGA TCTCCTCCAG
    AGGCGTTTCT CAGGCGGCAG TCAGTCATAT GATGGAGAAT CTCCTCGTCT TTCTCCCTGT
    AACAGCATGG GAAAACTCAG CAAATCTGAT GAGCAGCTTT CGTCTCTGGA GCGGGACAGT
    GGACAGTGCT CTCGCAACAC CAGCTGTGAA ACGCTTGAGG GATACGATCC AGATTACGAC
    TTTTTAAATC AGGATCTGTC CGTCTCGGAA CCGCTGCCGT TTCCCACCAA CACTGGCGGC
    AGTCTGAGCC CTCTTCCAGA GTGCAACACT GAGTCTCAAT CTCTAAGCCC CGCCCACATC
    CCTGCACGCC CACGCTTCAG CGCTCCAGTA ACCAATCAGG CCTCCCTAGA CTACTGGACT
    CCACCCCTCA CACCTGGACA GGGTAATAGC GTGCTTCCCC CTGCCCTCCC ACAGAAGAAG
    CGCCGCTCTG CACCAATCAT ATCGGCGTAT CCTCTGTACG AGCGCCTCCC GTCACACTAC
    GATAACCTAT CAGAGGAGGA GCAGCCGACG CCGCCGTTTC CACTGGTTAC ACCGGGGTCG
    CCGCAGGTCA ACGGAGACGG TGTTTTACAG TACACAGACG CTGACAGTCC ACCGCCTCTG
    CCGGAGAAAA AGGGCAAACA GATCCTGCAA TACATGCAGT TTGTGGAGGA TTACTCTGAG
    CCGCAGCCGT CCGTCTTCTA CCAGCTGCCT CAGAGCGAGA GTATTTACGA GCAGCGCAGG
    AACAAGCGCT TTCAGGAGGT TTACGGCCTC AACGACTCCT ACAGCAGCTC CGACTCCATT
    CCTGCTTCAG CTGCAGATCC GCACGAACGG GTCCCACCAC CAGCGCTGCC TCCAAAACTC
    AGACAACTGG CCTCCAACAC TACTACTTTT CCTCCTTCAT CCTCCTCTTC TGCTTTTTCA
    ACTCTTCCTC CTCCTGCTGG GCTTCCGGAG GAGGTGGAGC CTGCTCTTGG TCTCAGCCTA
    TCAGTGTCTA GCTCTTTCCT CAGTGGCCAC GCCTCTTTGA CCACTCCTGT GAGTTCCGAG
    CCGGTTGGCT TGACCAATAG CGGCAGAGCT CTGGATGGTG GAGGTGTCTC AAACGGGTCT
    TTGACCAGCT CTCTGCTCTC TCTGGCTGTC TGTCTTCCTT CTGAGTCGTC TCTCTCTGAC
    TCGCTTCTTA CCTCAGCGAG CGAAAGCATT GATGAAAGTG GTGAAGGAGA ATATGTAAAC
    CTGTACTCGT CCACACAGGC CAACGGAGAA AACACACACC GCACTGAATC TTTATCATGT
    GACGATGTGC TTCAAGACCA CTCCCCTCAC ATTCCCTCGT CCAATAGCAA GGAGGCTCTG
    ACTAAGGAGA GGCCGAAGGA AGCCAGTCAC GGCCACATTG ATGATGTGGA TGAATTATCC
    CTGATTGATC ATAATGACAT CATGAACCGC ATCACACTCA AAGCAGAGAA TGATGATGGT
    CCTGATGTGA GAGCTGGATC TGTAGACATA CTGTTAGTTC ACGCTACAGA AACAGACCGC
    AAAGATTTGG TGTTGTACTG CGAGGCGTTT CTCACAACCT ACAGAACCTT CATAACACCA
    GAAGACCTCA TTAAAAAGCT ACACCACAGA TATACTCGCT TCTGCCACAG TCCAGACACC
    TTTAAGAAGC GTGTCAGTAA AAACACCTTC TTTGTCCTGG TGAGAGTCGT GGATGAACTT
    TGTCTGGTAG AGTTGACCGA GGACATCCTG CGGCAGTTGA TGGATCTGGT GTTTCGGCTG
    GTCTGTAACG GTGAACTGAG TCTGGCGAGA GTCCTGCGGA AAAATATCCT GGATAAAGTG
    GAGCAGAAGA GACTCCTGCA GCACATGCAC ACACTCAGGC CTCTGGCGGC ACTCGGCGTC
    TCTGCCAGGC CAGGAACACT GCATGATTTC CGCAGTCATG AGATCGCCGA TCAGCTAACG
    TTGCTGGACG CAGAACTCTT TTACAAGATT GAGATTCCTG AAGTCCTGCT TTGGGCAAAG
    GAGCAGAACG AAGAGAAAAG TCCAAATCTG ACACAGTTTA CAGAGCACTT TAACAACATG
    AGCTACTGGG TGCGCTCTAT AATCATCCAG CAGGAGAAAG CTCAGGACAG AGAGAAGCTA
    CTGCTCAAAT TCATCAAAAT CATGAAGCAC TTGCGTAAGC TGAATAACTT CAACTCTTAC
    CTGGCGATTC TCTCGGCTCT GGACTCTGCA CCGATCAGAC GGCTGGAGTG GCAGAAACAG
    ACGTCTGAGG GTTTGGAGGA ATACTGCACT CTCATCGACA GCTCTTCGTC ATTCAGAGCA
    TACAGAGCAG CGTTGGCGGA AGTTGAGCCT CCATGTATAC CTTACCTCGG TTTGATCCTG
    CAGGATCTGA CGTTTGTTCA TCTGGGAAAC CCGGATTTTA TAGAGGGGAA AGTGAATTTC
    TCCAAGCGCT GGCAGCAGTT CAACATTCTG GACAGCATGA GACGCTTCCA GCAAGTACAC
    TACGACCTGA AGCGAAACGA CGACATCGTC TCGTTCTTCA ACGACTTTAG CGATCACCTG
    GCGGAGGAGG CATTATGGGA ACTCTCGCTC AAGATCAAAC CCCGAAACAT CTCGCGGCGC
    AGGACGGAGC GAGAGGAGAA GACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa40640
    Clone ID Related Accession (Same CDS sequence) XM_009295118.2
    Accession Version XM_009295118.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3504bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rap guanine nucleotide exchange factor 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295118.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCGGGGA AAATAGAAAG CAAGCACGAA TCCCAGAAGT CTCACCTGTC GTCCTTCACG 
    ATGAAACTGA TGAAGTTTCA TTCCCCGAAG ATTAAACGGA CCCCATCTAA GAAAGGCAAA
    CAACTCCAGC CTGAGCCTGC AGTGAAAACG CCAGAGAAGC CAGTCAACAA GAAGGTGAGT
    CGACTGGAGG AACAGGAGAA GGATGTGGTC AGCGCTCTGA GATACTTTAA GACGATTGTG
    GACAAGATGA ACGTTGACAC CAAAGTTCTC CAGATGCTTC CTGGTTCGGC TAGTAAAGTT
    TTAGAGGCTA TTTTGCCTCT GATGCAGGTC GAGGCTCGGA TACAGCACAG CTCCGCCATG
    TCCTCCTGTC ACAACCGTGT TTATCAGAGC TTGGCGACTC TGATTCGCTG GTCCGACCAG
    GTGATGCTGG AGGGCATCGA CCTCGACGAC AAGGACACTG TGACGACTGT TACCACGGTG
    ATCAAAGCTG TGCTGGATGG TGTGAAGGAG CTTGTGAAAC TCACTATAGA GAAACATGAC
    CAACCGTCCC CAACATCCCC CAGCAAACCA GCGCCTCCTG TGGCCAAAAC AGAGAGTGGG
    TGTGAAAATC CTCTAACCGA AAGAGAGAAG GCCATCCTGA GTAAGACCAC GCCCATTGTC
    ACGCCCACTG ACAGCTTGAC TGACATGTCA GAGGAGGAGG CTCCACCCAA ACCTCCTCTA
    CCAGGACCCA AACTAGCAGA GCACAGTCCA CCGGCTCTGC CTCCGAAGAA GCGTCAGTCG
    GCCCCGTCAC CCACCCGTGT GGCAGTGGTG GCCCCGATGA GCCGGGGTCC GTGTAGCCTC
    AGCCTGCCTC CTGGAGCCCT CAGACAGCAG CAGGACTTTG ATGTGGATCT CCTCCAGAGG
    CGTTTCTCAG GCGGCAGTCA GTCATATGAT GGAGAATCTC CTCGTCTTTC TCCCTGTAAC
    AGCATGGGAA AACTCAGCAA ATCTGATGAG CAGCTTTCGT CTCTGGAGCG GGACAGTGGA
    CAGTGCTCTC GCAACACCAG CTGTGAAACG CTTGAGGGAT ACGATCCAGA TTACGACTTT
    TTAAATCAGG ATCTGTCCGT CTCGGAACCG CTGCCGTTTC CCACCAACAC TGGCGGCAGT
    CTGAGCCCTC TTCCAGAGTG CAACACTGAG TCTCAATCTC TAAGCCCCGC CCACATCCCT
    GCACGCCCAC GCTTCAGCGC TCCAGTAACC AATCAGGCCT CCCTAGACTA CTGGACTCCA
    CCCCTCACAC CTGGACAGGG TAATAGCGTG CTTCCCCCTG CCCTCCCACA GAAGAAGCGC
    CGCTCTGCAC CAATCATATC GGCGTATCCT CTGTACGAGC GCCTCCCGTC ACACTACGAT
    AACCTATCAG AGGAGGAGCA GCCGACGCCG CCGTTTCCAC TGGTTACACC GGGGTCGCCG
    CAGGTCAACG GAGACGGTGT TTTACAGTAC ACAGACGCTG ACAGTCCACC GCCTCTGCCG
    GAGAAAAAGG GCAAACAGAT CCTGCAATAC ATGCAGTTTG TGGAGGATTA CTCTGAGCCG
    CAGCCGTCCG TCTTCTACCA GCTGCCTCAG AGCGAGAGTA TTTACGAGCA GCGCAGGAAC
    AAGCGCTTTC AGGAGGTTTA CGGCCTCAAC GACTCCTACA GCAGCTCCGA CTCCATTCCT
    GCTTCAGCTG CAGATCCGCA CGAACGGGTC CCACCACCAG CGCTGCCTCC AAAACTCAGA
    CAACTGGCCT CCAACACTAC TACTTTTCCT CCTTCATCCT CCTCTTCTGC TTTTTCAACT
    CTTCCTCCTC CTGCTGGGCT TCCGGAGGAG GTGGAGCCTG CTCTTGGTCT CAGCCTATCA
    GTGTCTAGCT CTTTCCTCAG TGGCCACGCC TCTTTGACCA CTCCTGTGAG TTCCGAGCCG
    GTTGGCTTGA CCAATAGCGG CAGAGCTCTG GATGGTGGAG GTGTCTCAAA CGGGTCTTTG
    ACCAGCTCTC TGCTCTCTCT GGCTGTCTGT CTTCCTTCTG AGTCGTCTCT CTCTGACTCG
    CTTCTTACCT CAGCGAGCGA AAGCATTGAT GAAAGTGGTG AAGGAGAATA TGTAAACCTG
    TACTCGTCCA CACAGGCCAA CGGAGAAAAC ACACACCGCA CTGAATCTTT ATCATGTGAC
    GATGTGCTTC AAGACCACTC CCCTCACATT CCCTCGTCCA ATAGCAAGGA GGCTCTGACT
    AAGGAGAGGC CGAAGGAAGC CAGTCACGGC CACATTGATG ATGTGGATGA ATTATCCCTG
    ATTGATCATA ATGACATCAT GAACCGCATC ACACTCAAAG CAGAGAATGA TGATGGTCCT
    GATGTGAGAG CTGGATCTGT AGACATACTG TTAGTTCACG CTACAGAAAC AGACCGCAAA
    GATTTGGTGT TGTACTGCGA GGCGTTTCTC ACAACCTACA GAACCTTCAT AACACCAGAA
    GACCTCATTA AAAAGCTACA CCACAGATAT ACTCGCTTCT GCCACAGTCC AGACACCTTT
    AAGAAGCGTG TCAGTAAAAA CACCTTCTTT GTCCTGGTGA GAGTCGTGGA TGAACTTTGT
    CTGGTAGAGT TGACCGAGGA CATCCTGCGG CAGTTGATGG ATCTGGTGTT TCGGCTGGTC
    TGTAACGGTG AACTGAGTCT GGCGAGAGTC CTGCGGAAAA ATATCCTGGA TAAAGTGGAG
    CAGAAGAGAC TCCTGCAGCA CATGCACACA CTCAGGCCTC TGGCGGCACT CGGCGTCTCT
    GCCAGGCCAG GAACACTGCA TGATTTCCGC AGTCATGAGA TCGCCGATCA GCTAACGTTG
    CTGGACGCAG AACTCTTTTA CAAGATTGAG ATTCCTGAAG TCCTGCTTTG GGCAAAGGAG
    CAGAACGAAG AGAAAAGTCC AAATCTGACA CAGTTTACAG AGCACTTTAA CAACATGAGC
    TACTGGGTGC GCTCTATAAT CATCCAGCAG GAGAAAGCTC AGGACAGAGA GAAGCTACTG
    CTCAAATTCA TCAAAATCAT GAAGCACTTG CGTAAGCTGA ATAACTTCAA CTCTTACCTG
    GCGATTCTCT CGGCTCTGGA CTCTGCACCG ATCAGACGGC TGGAGTGGCA GAAACAGACG
    TCTGAGGGTT TGGAGGAATA CTGCACTCTC ATCGACAGCT CTTCGTCATT CAGAGCATAC
    AGAGCAGCGT TGGCGGAAGT TGAGCCTCCA TGTATACCTT ACCTCGGTTT GATCCTGCAG
    GATCTGACGT TTGTTCATCT GGGAAACCCG GATTTTATAG AGGGGAAAGT GAATTTCTCC
    AAGCGCTGGC AGCAGTTCAA CATTCTGGAC AGCATGAGAC GCTTCCAGCA AGTACACTAC
    GACCTGAAGC GAAACGACGA CATCGTCTCG TTCTTCAACG ACTTTAGCGA TCACCTGGCG
    GAGGAGGCAT TATGGGAACT CTCGCTCAAG ATCAAACCCC GAAACATCTC GCGGCGCAGG
    ACGGAGCGAG AGGAGAAGAC CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009293393.1
    CDS293..3796
    Translation

    Target ORF information:

    RefSeq Version XM_009295118.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Rap guanine nucleotide exchange factor (GEF) 1b (rapgef1b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009295118.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTCGGGGA AAATAGAAAG CAAGCACGAA TCCCAGAAGT CTCACCTGTC GTCCTTCACG 
    ATGAAACTGA TGAAGTTTCA TTCCCCGAAG ATTAAACGGA CCCCATCTAA GAAAGGCAAA
    CAACTCCAGC CTGAGCCTGC AGTGAAAACG CCAGAGAAGC CAGTCAACAA GAAGGTGAGT
    CGACTGGAGG AACAGGAGAA GGATGTGGTC AGCGCTCTGA GATACTTTAA GACGATTGTG
    GACAAGATGA ACGTTGACAC CAAAGTTCTC CAGATGCTTC CTGGTTCGGC TAGTAAAGTT
    TTAGAGGCTA TTTTGCCTCT GATGCAGGTC GAGGCTCGGA TACAGCACAG CTCCGCCATG
    TCCTCCTGTC ACAACCGTGT TTATCAGAGC TTGGCGACTC TGATTCGCTG GTCCGACCAG
    GTGATGCTGG AGGGCATCGA CCTCGACGAC AAGGACACTG TGACGACTGT TACCACGGTG
    ATCAAAGCTG TGCTGGATGG TGTGAAGGAG CTTGTGAAAC TCACTATAGA GAAACATGAC
    CAACCGTCCC CAACATCCCC CAGCAAACCA GCGCCTCCTG TGGCCAAAAC AGAGAGTGGG
    TGTGAAAATC CTCTAACCGA AAGAGAGAAG GCCATCCTGA GTAAGACCAC GCCCATTGTC
    ACGCCCACTG ACAGCTTGAC TGACATGTCA GAGGAGGAGG CTCCACCCAA ACCTCCTCTA
    CCAGGACCCA AACTAGCAGA GCACAGTCCA CCGGCTCTGC CTCCGAAGAA GCGTCAGTCG
    GCCCCGTCAC CCACCCGTGT GGCAGTGGTG GCCCCGATGA GCCGGGGTCC GTGTAGCCTC
    AGCCTGCCTC CTGGAGCCCT CAGACAGCAG CAGGACTTTG ATGTGGATCT CCTCCAGAGG
    CGTTTCTCAG GCGGCAGTCA GTCATATGAT GGAGAATCTC CTCGTCTTTC TCCCTGTAAC
    AGCATGGGAA AACTCAGCAA ATCTGATGAG CAGCTTTCGT CTCTGGAGCG GGACAGTGGA
    CAGTGCTCTC GCAACACCAG CTGTGAAACG CTTGAGGGAT ACGATCCAGA TTACGACTTT
    TTAAATCAGG ATCTGTCCGT CTCGGAACCG CTGCCGTTTC CCACCAACAC TGGCGGCAGT
    CTGAGCCCTC TTCCAGAGTG CAACACTGAG TCTCAATCTC TAAGCCCCGC CCACATCCCT
    GCACGCCCAC GCTTCAGCGC TCCAGTAACC AATCAGGCCT CCCTAGACTA CTGGACTCCA
    CCCCTCACAC CTGGACAGGG TAATAGCGTG CTTCCCCCTG CCCTCCCACA GAAGAAGCGC
    CGCTCTGCAC CAATCATATC GGCGTATCCT CTGTACGAGC GCCTCCCGTC ACACTACGAT
    AACCTATCAG AGGAGGAGCA GCCGACGCCG CCGTTTCCAC TGGTTACACC GGGGTCGCCG
    CAGGTCAACG GAGACGGTGT TTTACAGTAC ACAGACGCTG ACAGTCCACC GCCTCTGCCG
    GAGAAAAAGG GCAAACAGAT CCTGCAATAC ATGCAGTTTG TGGAGGATTA CTCTGAGCCG
    CAGCCGTCCG TCTTCTACCA GCTGCCTCAG AGCGAGAGTA TTTACGAGCA GCGCAGGAAC
    AAGCGCTTTC AGGAGGTTTA CGGCCTCAAC GACTCCTACA GCAGCTCCGA CTCCATTCCT
    GCTTCAGCTG CAGATCCGCA CGAACGGGTC CCACCACCAG CGCTGCCTCC AAAACTCAGA
    CAACTGGCCT CCAACACTAC TACTTTTCCT CCTTCATCCT CCTCTTCTGC TTTTTCAACT
    CTTCCTCCTC CTGCTGGGCT TCCGGAGGAG GTGGAGCCTG CTCTTGGTCT CAGCCTATCA
    GTGTCTAGCT CTTTCCTCAG TGGCCACGCC TCTTTGACCA CTCCTGTGAG TTCCGAGCCG
    GTTGGCTTGA CCAATAGCGG CAGAGCTCTG GATGGTGGAG GTGTCTCAAA CGGGTCTTTG
    ACCAGCTCTC TGCTCTCTCT GGCTGTCTGT CTTCCTTCTG AGTCGTCTCT CTCTGACTCG
    CTTCTTACCT CAGCGAGCGA AAGCATTGAT GAAAGTGGTG AAGGAGAATA TGTAAACCTG
    TACTCGTCCA CACAGGCCAA CGGAGAAAAC ACACACCGCA CTGAATCTTT ATCATGTGAC
    GATGTGCTTC AAGACCACTC CCCTCACATT CCCTCGTCCA ATAGCAAGGA GGCTCTGACT
    AAGGAGAGGC CGAAGGAAGC CAGTCACGGC CACATTGATG ATGTGGATGA ATTATCCCTG
    ATTGATCATA ATGACATCAT GAACCGCATC ACACTCAAAG CAGAGAATGA TGATGGTCCT
    GATGTGAGAG CTGGATCTGT AGACATACTG TTAGTTCACG CTACAGAAAC AGACCGCAAA
    GATTTGGTGT TGTACTGCGA GGCGTTTCTC ACAACCTACA GAACCTTCAT AACACCAGAA
    GACCTCATTA AAAAGCTACA CCACAGATAT ACTCGCTTCT GCCACAGTCC AGACACCTTT
    AAGAAGCGTG TCAGTAAAAA CACCTTCTTT GTCCTGGTGA GAGTCGTGGA TGAACTTTGT
    CTGGTAGAGT TGACCGAGGA CATCCTGCGG CAGTTGATGG ATCTGGTGTT TCGGCTGGTC
    TGTAACGGTG AACTGAGTCT GGCGAGAGTC CTGCGGAAAA ATATCCTGGA TAAAGTGGAG
    CAGAAGAGAC TCCTGCAGCA CATGCACACA CTCAGGCCTC TGGCGGCACT CGGCGTCTCT
    GCCAGGCCAG GAACACTGCA TGATTTCCGC AGTCATGAGA TCGCCGATCA GCTAACGTTG
    CTGGACGCAG AACTCTTTTA CAAGATTGAG ATTCCTGAAG TCCTGCTTTG GGCAAAGGAG
    CAGAACGAAG AGAAAAGTCC AAATCTGACA CAGTTTACAG AGCACTTTAA CAACATGAGC
    TACTGGGTGC GCTCTATAAT CATCCAGCAG GAGAAAGCTC AGGACAGAGA GAAGCTACTG
    CTCAAATTCA TCAAAATCAT GAAGCACTTG CGTAAGCTGA ATAACTTCAA CTCTTACCTG
    GCGATTCTCT CGGCTCTGGA CTCTGCACCG ATCAGACGGC TGGAGTGGCA GAAACAGACG
    TCTGAGGGTT TGGAGGAATA CTGCACTCTC ATCGACAGCT CTTCGTCATT CAGAGCATAC
    AGAGCAGCGT TGGCGGAAGT TGAGCCTCCA TGTATACCTT ACCTCGGTTT GATCCTGCAG
    GATCTGACGT TTGTTCATCT GGGAAACCCG GATTTTATAG AGGGGAAAGT GAATTTCTCC
    AAGCGCTGGC AGCAGTTCAA CATTCTGGAC AGCATGAGAC GCTTCCAGCA AGTACACTAC
    GACCTGAAGC GAAACGACGA CATCGTCTCG TTCTTCAACG ACTTTAGCGA TCACCTGGCG
    GAGGAGGCAT TATGGGAACT CTCGCTCAAG ATCAAACCCC GAAACATCTC GCGGCGCAGG
    ACGGAGCGAG AGGAGAAGAC CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa40641
    Clone ID Related Accession (Same CDS sequence) XM_009295119.2
    Accession Version XM_009295119.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3405bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rap guanine nucleotide exchange factor 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295119.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCGGGGA AAATAGAAAG CAAGCACGAA TCCCAGAAGT CTCACCTGTC GTCCTTCACG 
    ATGAAACTGA TGAAGTTTCA TTCCCCGAAG ATTAAACGGA CCCCATCTAA GAAAGGCAAA
    CAACTCCAGC CTGAGCCTGC AGTGAAAACG CCAGAGAAGC CAGTCAACAA GAAGGTGAGT
    CGACTGGAGG AACAGGAGAA GGATGTGGTC AGCGCTCTGA GATACTTTAA GACGATTGTG
    GACAAGATGA ACGTTGACAC CAAAGTTCTC CAGATGCTTC CTGGTTCGGC TAGTAAAGTT
    TTAGAGGCTA TTTTGCCTCT GATGCAGGTC GAGGCTCGGA TACAGCACAG CTCCGCCATG
    TCCTCCTGTC ACAACCGTGT TTATCAGAGC TTGGCGACTC TGATTCGCTG GTCCGACCAG
    GTGATGCTGG AGGGCATCGA CCTCGACGAC AAGGACACTG TGACGACTGT TACCACGGTG
    ATCAAAGCTG TGCTGGATGG TGTGAAGGAG CTTGTGAAAC TCACTATAGA GAAACATGAC
    CAACCGTCCC CAACATCCCC CAGCAAACCA GCGCCTCCTG TGGCCAAAAC AGAGAGTGGG
    TGTGAAAATC CTCTAACCGA AAGAGAGAAG GCCATCCTGA GTAAGACCAC GCCCATTGTC
    ACGCCCACTG ACAGCTTGAC TGACATGTCA GAGGAGGAGG CTCCACCCAA ACCTCCTCTA
    CCAGGACCCA AACTAGCAGA GCACAGGAAC AGGATGCAGT TGTGCTCCGA TCCTGTTACC
    GGGCAGCGGA GGGCTACGAT GATGGAAACT CCACCGGCTC TGCCTCCGAA GAAGCGTCAG
    TCGGCCCCGT CACCCACCCG TGTGGCAGTG GTGGCCCCGA TGAGCCGGGG TCCGTGTAGC
    CTCAGCCTGC CTCCTGGAGC CCTCAGACAG CAGCAGGACT TTGATGTGGA TCTCCTCCAG
    AGGCGTTTCT CAGGCGGCAG TCAGTCATAT GATGGAGAAT CTCCTCGTCT TTCTCCCTGT
    AACAGCATGG GAAAACTCAG CAAATCTGAT GAGCAGCTTT CGTCTCTGGA GCGGGACAGT
    GGACAGTGCT CTCGCAACAC CAGCTGTGAA ACGCTTGAGG GATACGATCC AGATTACGAC
    TTTTTAAATC AGGATCTGTC CGTCTCGGAA CCGCTGCCGT TTCCCACCAA CACTGGCGGC
    AGTCTGAGCC CTCTTCCAGA GTGCAACACT GAGTCTCAAT CTCTAAGCCC CGCCCACATC
    CCTGCACGCC CACGCTTCAG CGCTCCAGTA ACCAATCAGG CCTCCCTAGA CTACTGGACT
    CCACCCCTCA CACCTGGACA GGGTAATAGC GTGCTTCCCC CTGCCCTCCC ACAGAAGAAG
    CGCCGCTCTG CACCAATCAT ATCGGCGTAT CCTCTGTACG AGCGCCTCCC GTCACACTAC
    GATAACCTAT CAGAGGAGGA GCAGCCGACG CCGCCGTTTC CACTGGTTAC ACCGGGGTCG
    CCGCAGGTCA ACGGAGACGG TGTTTTACAG TACACAGACG CTGACAGTCC ACCGCCTCTG
    CCGGAGAAAA AGGGCAAACA GATCCTGCAA TACATGCAGT TTGTGGAGGA TTACTCTGAG
    CCGCAGCCGT CCGTCTTCTA CCAGCTGCCT CAGAGCGAGA GTATTTACGA GCAGCGCAGG
    AACAAGCGCT TTCAGGAGGT TTACGGCCTC AACGACTCCT ACAGCAGCTC CGACTCCATT
    CCTGCTTCAG CTGCAGATCC GCACGAACGG GTCCCACCAC CAGCGCTGCC TCCAAAACTC
    AGACAACTGA GTTCCGAGCC GGTTGGCTTG ACCAATAGCG GCAGAGCTCT GGATGGTGGA
    GGTGTCTCAA ACGGGTCTTT GACCAGCTCT CTGCTCTCTC TGGCTGTCTG TCTTCCTTCT
    GAGTCGTCTC TCTCTGACTC GCTTCTTACC TCAGCGAGCG AAAGCATTGA TGAAAGTGGT
    GAAGGAGAAT ATGTAAACCT GTACTCGTCC ACACAGGCCA ACGGAGAAAA CACACACCGC
    ACTGAATCTT TATCATGTGA CGATGTGCTT CAAGACCACT CCCCTCACAT TCCCTCGTCC
    AATAGCAAGG AGGCTCTGAC TAAGGAGAGG CCGAAGGAAG CCAGTCACGG CCACATTGAT
    GATGTGGATG AATTATCCCT GATTGATCAT AATGACATCA TGAACCGCAT CACACTCAAA
    GCAGAGAATG ATGATGGTCC TGATGTGAGA GCTGGATCTG TAGACATACT GTTAGTTCAC
    GCTACAGAAA CAGACCGCAA AGATTTGGTG TTGTACTGCG AGGCGTTTCT CACAACCTAC
    AGAACCTTCA TAACACCAGA AGACCTCATT AAAAAGCTAC ACCACAGATA TACTCGCTTC
    TGCCACAGTC CAGACACCTT TAAGAAGCGT GTCAGTAAAA ACACCTTCTT TGTCCTGGTG
    AGAGTCGTGG ATGAACTTTG TCTGGTAGAG TTGACCGAGG ACATCCTGCG GCAGTTGATG
    GATCTGGTGT TTCGGCTGGT CTGTAACGGT GAACTGAGTC TGGCGAGAGT CCTGCGGAAA
    AATATCCTGG ATAAAGTGGA GCAGAAGAGA CTCCTGCAGC ACATGCACAC ACTCAGGCCT
    CTGGCGGCAC TCGGCGTCTC TGCCAGGCCA GGAACACTGC ATGATTTCCG CAGTCATGAG
    ATCGCCGATC AGCTAACGTT GCTGGACGCA GAACTCTTTT ACAAGATTGA GATTCCTGAA
    GTCCTGCTTT GGGCAAAGGA GCAGAACGAA GAGAAAAGTC CAAATCTGAC ACAGTTTACA
    GAGCACTTTA ACAACATGAG CTACTGGGTG CGCTCTATAA TCATCCAGCA GGAGAAAGCT
    CAGGACAGAG AGAAGCTACT GCTCAAATTC ATCAAAATCA TGAAGCACTT GCGTAAGCTG
    AATAACTTCA ACTCTTACCT GGCGATTCTC TCGGCTCTGG ACTCTGCACC GATCAGACGG
    CTGGAGTGGC AGAAACAGAC GTCTGAGGGT TTGGAGGAAT ACTGCACTCT CATCGACAGC
    TCTTCGTCAT TCAGAGCATA CAGAGCAGCG TTGGCGGAAG TTGAGCCTCC ATGTATACCT
    TACCTCGGTT TGATCCTGCA GGATCTGACG TTTGTTCATC TGGGAAACCC GGATTTTATA
    GAGGGGAAAG TGAATTTCTC CAAGCGCTGG CAGCAGTTCA ACATTCTGGA CAGCATGAGA
    CGCTTCCAGC AAGTACACTA CGACCTGAAG CGAAACGACG ACATCGTCTC GTTCTTCAAC
    GACTTTAGCG ATCACCTGGC GGAGGAGGCA TTATGGGAAC TCTCGCTCAA GATCAAACCC
    CGAAACATCT CGCGGCGCAG GACGGAGCGA GAGGAGAAGA CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009293394.1
    CDS293..3697
    Translation

    Target ORF information:

    RefSeq Version XM_009295119.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Rap guanine nucleotide exchange factor (GEF) 1b (rapgef1b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009295119.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCGGGGA AAATAGAAAG CAAGCACGAA TCCCAGAAGT CTCACCTGTC GTCCTTCACG 
    ATGAAACTGA TGAAGTTTCA TTCCCCGAAG ATTAAACGGA CCCCATCTAA GAAAGGCAAA
    CAACTCCAGC CTGAGCCTGC AGTGAAAACG CCAGAGAAGC CAGTCAACAA GAAGGTGAGT
    CGACTGGAGG AACAGGAGAA GGATGTGGTC AGCGCTCTGA GATACTTTAA GACGATTGTG
    GACAAGATGA ACGTTGACAC CAAAGTTCTC CAGATGCTTC CTGGTTCGGC TAGTAAAGTT
    TTAGAGGCTA TTTTGCCTCT GATGCAGGTC GAGGCTCGGA TACAGCACAG CTCCGCCATG
    TCCTCCTGTC ACAACCGTGT TTATCAGAGC TTGGCGACTC TGATTCGCTG GTCCGACCAG
    GTGATGCTGG AGGGCATCGA CCTCGACGAC AAGGACACTG TGACGACTGT TACCACGGTG
    ATCAAAGCTG TGCTGGATGG TGTGAAGGAG CTTGTGAAAC TCACTATAGA GAAACATGAC
    CAACCGTCCC CAACATCCCC CAGCAAACCA GCGCCTCCTG TGGCCAAAAC AGAGAGTGGG
    TGTGAAAATC CTCTAACCGA AAGAGAGAAG GCCATCCTGA GTAAGACCAC GCCCATTGTC
    ACGCCCACTG ACAGCTTGAC TGACATGTCA GAGGAGGAGG CTCCACCCAA ACCTCCTCTA
    CCAGGACCCA AACTAGCAGA GCACAGGAAC AGGATGCAGT TGTGCTCCGA TCCTGTTACC
    GGGCAGCGGA GGGCTACGAT GATGGAAACT CCACCGGCTC TGCCTCCGAA GAAGCGTCAG
    TCGGCCCCGT CACCCACCCG TGTGGCAGTG GTGGCCCCGA TGAGCCGGGG TCCGTGTAGC
    CTCAGCCTGC CTCCTGGAGC CCTCAGACAG CAGCAGGACT TTGATGTGGA TCTCCTCCAG
    AGGCGTTTCT CAGGCGGCAG TCAGTCATAT GATGGAGAAT CTCCTCGTCT TTCTCCCTGT
    AACAGCATGG GAAAACTCAG CAAATCTGAT GAGCAGCTTT CGTCTCTGGA GCGGGACAGT
    GGACAGTGCT CTCGCAACAC CAGCTGTGAA ACGCTTGAGG GATACGATCC AGATTACGAC
    TTTTTAAATC AGGATCTGTC CGTCTCGGAA CCGCTGCCGT TTCCCACCAA CACTGGCGGC
    AGTCTGAGCC CTCTTCCAGA GTGCAACACT GAGTCTCAAT CTCTAAGCCC CGCCCACATC
    CCTGCACGCC CACGCTTCAG CGCTCCAGTA ACCAATCAGG CCTCCCTAGA CTACTGGACT
    CCACCCCTCA CACCTGGACA GGGTAATAGC GTGCTTCCCC CTGCCCTCCC ACAGAAGAAG
    CGCCGCTCTG CACCAATCAT ATCGGCGTAT CCTCTGTACG AGCGCCTCCC GTCACACTAC
    GATAACCTAT CAGAGGAGGA GCAGCCGACG CCGCCGTTTC CACTGGTTAC ACCGGGGTCG
    CCGCAGGTCA ACGGAGACGG TGTTTTACAG TACACAGACG CTGACAGTCC ACCGCCTCTG
    CCGGAGAAAA AGGGCAAACA GATCCTGCAA TACATGCAGT TTGTGGAGGA TTACTCTGAG
    CCGCAGCCGT CCGTCTTCTA CCAGCTGCCT CAGAGCGAGA GTATTTACGA GCAGCGCAGG
    AACAAGCGCT TTCAGGAGGT TTACGGCCTC AACGACTCCT ACAGCAGCTC CGACTCCATT
    CCTGCTTCAG CTGCAGATCC GCACGAACGG GTCCCACCAC CAGCGCTGCC TCCAAAACTC
    AGACAACTGA GTTCCGAGCC GGTTGGCTTG ACCAATAGCG GCAGAGCTCT GGATGGTGGA
    GGTGTCTCAA ACGGGTCTTT GACCAGCTCT CTGCTCTCTC TGGCTGTCTG TCTTCCTTCT
    GAGTCGTCTC TCTCTGACTC GCTTCTTACC TCAGCGAGCG AAAGCATTGA TGAAAGTGGT
    GAAGGAGAAT ATGTAAACCT GTACTCGTCC ACACAGGCCA ACGGAGAAAA CACACACCGC
    ACTGAATCTT TATCATGTGA CGATGTGCTT CAAGACCACT CCCCTCACAT TCCCTCGTCC
    AATAGCAAGG AGGCTCTGAC TAAGGAGAGG CCGAAGGAAG CCAGTCACGG CCACATTGAT
    GATGTGGATG AATTATCCCT GATTGATCAT AATGACATCA TGAACCGCAT CACACTCAAA
    GCAGAGAATG ATGATGGTCC TGATGTGAGA GCTGGATCTG TAGACATACT GTTAGTTCAC
    GCTACAGAAA CAGACCGCAA AGATTTGGTG TTGTACTGCG AGGCGTTTCT CACAACCTAC
    AGAACCTTCA TAACACCAGA AGACCTCATT AAAAAGCTAC ACCACAGATA TACTCGCTTC
    TGCCACAGTC CAGACACCTT TAAGAAGCGT GTCAGTAAAA ACACCTTCTT TGTCCTGGTG
    AGAGTCGTGG ATGAACTTTG TCTGGTAGAG TTGACCGAGG ACATCCTGCG GCAGTTGATG
    GATCTGGTGT TTCGGCTGGT CTGTAACGGT GAACTGAGTC TGGCGAGAGT CCTGCGGAAA
    AATATCCTGG ATAAAGTGGA GCAGAAGAGA CTCCTGCAGC ACATGCACAC ACTCAGGCCT
    CTGGCGGCAC TCGGCGTCTC TGCCAGGCCA GGAACACTGC ATGATTTCCG CAGTCATGAG
    ATCGCCGATC AGCTAACGTT GCTGGACGCA GAACTCTTTT ACAAGATTGA GATTCCTGAA
    GTCCTGCTTT GGGCAAAGGA GCAGAACGAA GAGAAAAGTC CAAATCTGAC ACAGTTTACA
    GAGCACTTTA ACAACATGAG CTACTGGGTG CGCTCTATAA TCATCCAGCA GGAGAAAGCT
    CAGGACAGAG AGAAGCTACT GCTCAAATTC ATCAAAATCA TGAAGCACTT GCGTAAGCTG
    AATAACTTCA ACTCTTACCT GGCGATTCTC TCGGCTCTGG ACTCTGCACC GATCAGACGG
    CTGGAGTGGC AGAAACAGAC GTCTGAGGGT TTGGAGGAAT ACTGCACTCT CATCGACAGC
    TCTTCGTCAT TCAGAGCATA CAGAGCAGCG TTGGCGGAAG TTGAGCCTCC ATGTATACCT
    TACCTCGGTT TGATCCTGCA GGATCTGACG TTTGTTCATC TGGGAAACCC GGATTTTATA
    GAGGGGAAAG TGAATTTCTC CAAGCGCTGG CAGCAGTTCA ACATTCTGGA CAGCATGAGA
    CGCTTCCAGC AAGTACACTA CGACCTGAAG CGAAACGACG ACATCGTCTC GTTCTTCAAC
    GACTTTAGCG ATCACCTGGC GGAGGAGGCA TTATGGGAAC TCTCGCTCAA GATCAAACCC
    CGAAACATCT CGCGGCGCAG GACGGAGCGA GAGGAGAAGA CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa40642
    Clone ID Related Accession (Same CDS sequence) XM_009295120.2
    Accession Version XM_009295120.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3258bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rap guanine nucleotide exchange factor 1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295120.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTCGGGGA AAATAGAAAG CAAGCACGAA TCCCAGAAGT CTCACCTGTC GTCCTTCACG 
    ATGAAACTGA TGAAGTTTCA TTCCCCGAAG ATTAAACGGA CCCCATCTAA GAAAGGCAAA
    CAACTCCAGC CTGAGCCTGC AGTGAAAACG CCAGAGAAGC CAGTCAACAA GAAGGTGAGT
    CGACTGGAGG AACAGGAGAA GGATGTGGTC AGCGCTCTGA GATACTTTAA GACGATTGTG
    GACAAGATGA ACGTTGACAC CAAAGTTCTC CAGATGCTTC CTGGTTCGGC TAGTAAAGTT
    TTAGAGGCTA TTTTGCCTCT GATGCAGGTC GAGGCTCGGA TACAGCACAG CTCCGCCATG
    TCCTCCTGTC ACAACCGTGT TTATCAGAGC TTGGCGACTC TGATTCGCTG GTCCGACCAG
    GTGATGCTGG AGGGCATCGA CCTCGACGAC AAGGACACTG TGACGACTGT TACCACGGTG
    ATCAAAGCTG TGCTGGATGG TGTGAAGGAG CTTGTGAAAC TCACTATAGA GAAACATGAC
    CAACCGTCCC CAACATCCCC CAGCAAACCA GCGCCTCCTG TGGCCAAAAC AGAGAGTGGG
    TGTGAAAATC CTCTAACCGA AAGAGAGAAG GCCATCCTGA GTAAGACCAC GCCCATTGTC
    ACGCCCACTG ACAGCTTGAC TGACATGTCA GAGGAGGAGG CTCCACCCAA ACCTCCTCTA
    CCAGGACCCA AACTAGCAGA GCACAGGAAC AGGATGCAGT TGTGCTCCGA TCCTGTTACC
    GGGCAGCGGA GGGCTACGAT GATGGAAACT CCACCGGCTC TGCCTCCGAA GAAGCGTCAG
    TCGGCCCCGT CACCCACCCG TGTGGCAGTG GTGGCCCCGA TGAGCCGGGG TCCGTGTAGC
    CTCAGCCTGC CTCCTGGAGC CCTCAGACAG CAGCAGGACT TTGATGTGGA TCTCCTCCAG
    AGGCGTTTCT CAGGCGGCAG TCAGTCATAT GATGGAGAAT CTCCTCGTCT TTCTCCCTGT
    AACAGCATGG GAAAACTCAG CAAATCTGAT GAGCAGCTTT CGTCTCTGGA GCGGGACAGT
    GGACAGTGCT CTCGCAACAC CAGCTGTGAA ACGCTTGAGG GATACGATCC AGATTACGAC
    TTTTTAAATC AGGATCTGTC CGTCTCGGAA CCGCTGCCGT TTCCCACCAA CACTGGCGGC
    AGTCTGAGCC CTCTTCCAGA GTGCAACACT GAGTCTCAAT CTCTAAGCCC CGCCCACATC
    CCTGCACGCC CACGCTTCAG CGCTCCAGTA ACCAATCAGG CCTCCCTAGA CTACTGGACT
    CCACCCCTCA CACCTGGACA GGGTAATAGC GTGCTTCCCC CTGCCCTCCC ACAGAAGAAG
    CGCCGCTCTG CACCAATCAT ATCGGCGTAT CCTCTGTACG AGCGCCTCCC GTCACACTAC
    GATAACCTAT CAGAGGAGGA GCAGCCGACG CCGCCGTTTC CACTGGTTAC ACCGGGGTCG
    CCGCAGGTCA ACGGAGACGG TGTTTTACAG TACACAGACG CTGACAGTCC ACCGCCTCTG
    CCGGAGAAAA AGGGCAAACA GATCCTGCAA TACATGCAGT TTGTGGAGGA TTACTCTGAG
    CCGCAGCCGT CCGTCTTCTA CCAGCTGCCT CAGAGCGAGA GTATTTACGA GCAGCGCAGG
    AACAAGCGCT TTCAGGAGGT TTACGGCCTC AACGACTCCT ACAGCAGCTC CGACTCCATT
    CCTGCTTCAG CTGCAGATCC GCACGAACGG GTCCCACCAC CAGCGCTGCC TCCAAAACTC
    AGACAACTGA GCGAAAGCAT TGATGAAAGT GGTGAAGGAG AATATGTAAA CCTGTACTCG
    TCCACACAGG CCAACGGAGA AAACACACAC CGCACTGAAT CTTTATCATG TGACGATGTG
    CTTCAAGACC ACTCCCCTCA CATTCCCTCG TCCAATAGCA AGGAGGCTCT GACTAAGGAG
    AGGCCGAAGG AAGCCAGTCA CGGCCACATT GATGATGTGG ATGAATTATC CCTGATTGAT
    CATAATGACA TCATGAACCG CATCACACTC AAAGCAGAGA ATGATGATGG TCCTGATGTG
    AGAGCTGGAT CTGTAGACAT ACTGTTAGTT CACGCTACAG AAACAGACCG CAAAGATTTG
    GTGTTGTACT GCGAGGCGTT TCTCACAACC TACAGAACCT TCATAACACC AGAAGACCTC
    ATTAAAAAGC TACACCACAG ATATACTCGC TTCTGCCACA GTCCAGACAC CTTTAAGAAG
    CGTGTCAGTA AAAACACCTT CTTTGTCCTG GTGAGAGTCG TGGATGAACT TTGTCTGGTA
    GAGTTGACCG AGGACATCCT GCGGCAGTTG ATGGATCTGG TGTTTCGGCT GGTCTGTAAC
    GGTGAACTGA GTCTGGCGAG AGTCCTGCGG AAAAATATCC TGGATAAAGT GGAGCAGAAG
    AGACTCCTGC AGCACATGCA CACACTCAGG CCTCTGGCGG CACTCGGCGT CTCTGCCAGG
    CCAGGAACAC TGCATGATTT CCGCAGTCAT GAGATCGCCG ATCAGCTAAC GTTGCTGGAC
    GCAGAACTCT TTTACAAGAT TGAGATTCCT GAAGTCCTGC TTTGGGCAAA GGAGCAGAAC
    GAAGAGAAAA GTCCAAATCT GACACAGTTT ACAGAGCACT TTAACAACAT GAGCTACTGG
    GTGCGCTCTA TAATCATCCA GCAGGAGAAA GCTCAGGACA GAGAGAAGCT ACTGCTCAAA
    TTCATCAAAA TCATGAAGCA CTTGCGTAAG CTGAATAACT TCAACTCTTA CCTGGCGATT
    CTCTCGGCTC TGGACTCTGC ACCGATCAGA CGGCTGGAGT GGCAGAAACA GACGTCTGAG
    GGTTTGGAGG AATACTGCAC TCTCATCGAC AGCTCTTCGT CATTCAGAGC ATACAGAGCA
    GCGTTGGCGG AAGTTGAGCC TCCATGTATA CCTTACCTCG GTTTGATCCT GCAGGATCTG
    ACGTTTGTTC ATCTGGGAAA CCCGGATTTT ATAGAGGGGA AAGTGAATTT CTCCAAGCGC
    TGGCAGCAGT TCAACATTCT GGACAGCATG AGACGCTTCC AGCAAGTACA CTACGACCTG
    AAGCGAAACG ACGACATCGT CTCGTTCTTC AACGACTTTA GCGATCACCT GGCGGAGGAG
    GCATTATGGG AACTCTCGCT CAAGATCAAA CCCCGAAACA TCTCGCGGCG CAGGACGGAG
    CGAGAGGAGA AGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009293395.1
    CDS293..3550
    Translation

    Target ORF information:

    RefSeq Version XM_009295120.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Rap guanine nucleotide exchange factor (GEF) 1b (rapgef1b), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009295120.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTCGGGGA AAATAGAAAG CAAGCACGAA TCCCAGAAGT CTCACCTGTC GTCCTTCACG 
    ATGAAACTGA TGAAGTTTCA TTCCCCGAAG ATTAAACGGA CCCCATCTAA GAAAGGCAAA
    CAACTCCAGC CTGAGCCTGC AGTGAAAACG CCAGAGAAGC CAGTCAACAA GAAGGTGAGT
    CGACTGGAGG AACAGGAGAA GGATGTGGTC AGCGCTCTGA GATACTTTAA GACGATTGTG
    GACAAGATGA ACGTTGACAC CAAAGTTCTC CAGATGCTTC CTGGTTCGGC TAGTAAAGTT
    TTAGAGGCTA TTTTGCCTCT GATGCAGGTC GAGGCTCGGA TACAGCACAG CTCCGCCATG
    TCCTCCTGTC ACAACCGTGT TTATCAGAGC TTGGCGACTC TGATTCGCTG GTCCGACCAG
    GTGATGCTGG AGGGCATCGA CCTCGACGAC AAGGACACTG TGACGACTGT TACCACGGTG
    ATCAAAGCTG TGCTGGATGG TGTGAAGGAG CTTGTGAAAC TCACTATAGA GAAACATGAC
    CAACCGTCCC CAACATCCCC CAGCAAACCA GCGCCTCCTG TGGCCAAAAC AGAGAGTGGG
    TGTGAAAATC CTCTAACCGA AAGAGAGAAG GCCATCCTGA GTAAGACCAC GCCCATTGTC
    ACGCCCACTG ACAGCTTGAC TGACATGTCA GAGGAGGAGG CTCCACCCAA ACCTCCTCTA
    CCAGGACCCA AACTAGCAGA GCACAGGAAC AGGATGCAGT TGTGCTCCGA TCCTGTTACC
    GGGCAGCGGA GGGCTACGAT GATGGAAACT CCACCGGCTC TGCCTCCGAA GAAGCGTCAG
    TCGGCCCCGT CACCCACCCG TGTGGCAGTG GTGGCCCCGA TGAGCCGGGG TCCGTGTAGC
    CTCAGCCTGC CTCCTGGAGC CCTCAGACAG CAGCAGGACT TTGATGTGGA TCTCCTCCAG
    AGGCGTTTCT CAGGCGGCAG TCAGTCATAT GATGGAGAAT CTCCTCGTCT TTCTCCCTGT
    AACAGCATGG GAAAACTCAG CAAATCTGAT GAGCAGCTTT CGTCTCTGGA GCGGGACAGT
    GGACAGTGCT CTCGCAACAC CAGCTGTGAA ACGCTTGAGG GATACGATCC AGATTACGAC
    TTTTTAAATC AGGATCTGTC CGTCTCGGAA CCGCTGCCGT TTCCCACCAA CACTGGCGGC
    AGTCTGAGCC CTCTTCCAGA GTGCAACACT GAGTCTCAAT CTCTAAGCCC CGCCCACATC
    CCTGCACGCC CACGCTTCAG CGCTCCAGTA ACCAATCAGG CCTCCCTAGA CTACTGGACT
    CCACCCCTCA CACCTGGACA GGGTAATAGC GTGCTTCCCC CTGCCCTCCC ACAGAAGAAG
    CGCCGCTCTG CACCAATCAT ATCGGCGTAT CCTCTGTACG AGCGCCTCCC GTCACACTAC
    GATAACCTAT CAGAGGAGGA GCAGCCGACG CCGCCGTTTC CACTGGTTAC ACCGGGGTCG
    CCGCAGGTCA ACGGAGACGG TGTTTTACAG TACACAGACG CTGACAGTCC ACCGCCTCTG
    CCGGAGAAAA AGGGCAAACA GATCCTGCAA TACATGCAGT TTGTGGAGGA TTACTCTGAG
    CCGCAGCCGT CCGTCTTCTA CCAGCTGCCT CAGAGCGAGA GTATTTACGA GCAGCGCAGG
    AACAAGCGCT TTCAGGAGGT TTACGGCCTC AACGACTCCT ACAGCAGCTC CGACTCCATT
    CCTGCTTCAG CTGCAGATCC GCACGAACGG GTCCCACCAC CAGCGCTGCC TCCAAAACTC
    AGACAACTGA GCGAAAGCAT TGATGAAAGT GGTGAAGGAG AATATGTAAA CCTGTACTCG
    TCCACACAGG CCAACGGAGA AAACACACAC CGCACTGAAT CTTTATCATG TGACGATGTG
    CTTCAAGACC ACTCCCCTCA CATTCCCTCG TCCAATAGCA AGGAGGCTCT GACTAAGGAG
    AGGCCGAAGG AAGCCAGTCA CGGCCACATT GATGATGTGG ATGAATTATC CCTGATTGAT
    CATAATGACA TCATGAACCG CATCACACTC AAAGCAGAGA ATGATGATGG TCCTGATGTG
    AGAGCTGGAT CTGTAGACAT ACTGTTAGTT CACGCTACAG AAACAGACCG CAAAGATTTG
    GTGTTGTACT GCGAGGCGTT TCTCACAACC TACAGAACCT TCATAACACC AGAAGACCTC
    ATTAAAAAGC TACACCACAG ATATACTCGC TTCTGCCACA GTCCAGACAC CTTTAAGAAG
    CGTGTCAGTA AAAACACCTT CTTTGTCCTG GTGAGAGTCG TGGATGAACT TTGTCTGGTA
    GAGTTGACCG AGGACATCCT GCGGCAGTTG ATGGATCTGG TGTTTCGGCT GGTCTGTAAC
    GGTGAACTGA GTCTGGCGAG AGTCCTGCGG AAAAATATCC TGGATAAAGT GGAGCAGAAG
    AGACTCCTGC AGCACATGCA CACACTCAGG CCTCTGGCGG CACTCGGCGT CTCTGCCAGG
    CCAGGAACAC TGCATGATTT CCGCAGTCAT GAGATCGCCG ATCAGCTAAC GTTGCTGGAC
    GCAGAACTCT TTTACAAGAT TGAGATTCCT GAAGTCCTGC TTTGGGCAAA GGAGCAGAAC
    GAAGAGAAAA GTCCAAATCT GACACAGTTT ACAGAGCACT TTAACAACAT GAGCTACTGG
    GTGCGCTCTA TAATCATCCA GCAGGAGAAA GCTCAGGACA GAGAGAAGCT ACTGCTCAAA
    TTCATCAAAA TCATGAAGCA CTTGCGTAAG CTGAATAACT TCAACTCTTA CCTGGCGATT
    CTCTCGGCTC TGGACTCTGC ACCGATCAGA CGGCTGGAGT GGCAGAAACA GACGTCTGAG
    GGTTTGGAGG AATACTGCAC TCTCATCGAC AGCTCTTCGT CATTCAGAGC ATACAGAGCA
    GCGTTGGCGG AAGTTGAGCC TCCATGTATA CCTTACCTCG GTTTGATCCT GCAGGATCTG
    ACGTTTGTTC ATCTGGGAAA CCCGGATTTT ATAGAGGGGA AAGTGAATTT CTCCAAGCGC
    TGGCAGCAGT TCAACATTCT GGACAGCATG AGACGCTTCC AGCAAGTACA CTACGACCTG
    AAGCGAAACG ACGACATCGT CTCGTTCTTC AACGACTTTA GCGATCACCT GGCGGAGGAG
    GCATTATGGG AACTCTCGCT CAAGATCAAA CCCCGAAACA TCTCGCGGCG CAGGACGGAG
    CGAGAGGAGA AGACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    RFX transcription factors are essential for hearing in mice.
    Nature communications68549(2015 Oct)
    Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R


    Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
    Proceedings of the National Academy of Sciences of the United States of America99(26)16899-903(2002 Dec)
    Strausberg RL,Feingold EA,Grouse LH,Derge JG,Klausner RD,Collins FS,Wagner L,Shenmen CM,Schuler GD,Altschul SF,Zeeberg B,Buetow KH,Schaefer CF,Bhat NK,Hopkins RF,Jordan H,Moore T,Max SI,Wang J,Hsieh F,Diatchenko L,Marusina K,Farmer AA,Rubin GM,Hong L,Stapleton M,Soares MB,Bonaldo MF,Casavant TL,Scheetz TE,Brownstein MJ,Usdin TB,Toshiyuki S,Carninci P,Prange C,Raha SS,Loquellano NA,Peters GJ,Abramson RD,Mullahy SJ,Bosak SA,McEwan PJ,McKernan KJ,Malek JA,Gunaratne PH,Richards S,Worley KC,Hale S,Garcia AM,Gay LJ,Hulyk SW,Villalon DK,Muzny DM,Sodergren EJ,Lu X,Gibbs RA,Fahey J,Helton E,Ketteman M,Madan A,Rodrigues S,Sanchez A,Whiting M,Madan A,Young AC,Shevchenko Y,Bouffard GG,Blakesley RW,Touchman JW,Green ED,Dickson MC,Rodriguez AC,Grimwood J,Schmutz J,Myers RM,Butterfield YS,Krzywinski MI,Skalska U,Smailus DE,Schnerch A,Schein JE,Jones SJ,Marra MA,