pycr1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	pycr1a
Entrez Gene ID	393799
Full Name	pyrroline-5-carboxylate reductase 1a
Synonyms	pycr1,zgc:73112
General protein information	Preferred Names pyrroline-5-carboxylate reductase 1a Names pyrroline-5-carboxylate reductase 1, mitochondrial pyrroline-5-carboxylate reductase 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021469847.1	XP_021325522.1	pyrroline-5-carboxylate reductase 1, mitochondrial isoform X1
NM_200826.1	NP_957120.1	pyrroline-5-carboxylate reductase 1, mitochondrial

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Pycr2	NP_001012208.1
Pan troglodytes (chimpanzee)	PYCR2	XP_514237.2
Canis lupus familiaris (dog)	PYCR2	XP_537234.3
Danio rerio (zebrafish)	pycr1a	NP_957120.1
Xenopus tropicalis (tropical clawed frog)	pycr1	NP_001015942.1
Homo sapiens (human)	PYCR2	NP_037460.2
	PYCR2	XP_001092867.1
Bos taurus (cattle)	PYCR2	NP_001068649.1
Mus musculus (house mouse)	Pycr2	NP_598466.1
Gallus gallus (chicken)	PYCR2	XP_001232432.2
Danio rerio (zebrafish)	pycr1b	NP_001018454.1

Gene Ontology
Bibliography

Related articles in PubMed

A rapid and effective method for screening, sequencing and reporter verification of engineered frameshift mutations in zebrafish.
Prykhozhij SV, Steele SL, Razaghi B, Berman JN
Disease models & mechanisms10(6)811-822(2017 Jun)

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Mutations in PYCR2, Encoding Pyrroline-5-Carboxylate Reductase 2, Cause Microcephaly and Hypomyelination.
Nakayama T, Al-Maawali A, El-Quessny M, Rajab A, Khalil S, Stoler JM, Tan WH, Nasir R, Schmitz-Abe K, Hill RS, Partlow JN, Al-Saffar M, Servattalab S, LaCoursiere CM, Tambunan DE, Coulter ME, Elhosary PC, Gorski G, Barkovich AJ, Markianos K, Poduri A, Mochida GH
American journal of human genetics96(5)709-19(2015 May)

Mutations in PYCR1 cause cutis laxa with progeroid features.
Reversade B, Escande-Beillard N, Dimopoulou A, Fischer B, Chng SC, Li Y, Shboul M, Tham PY, Kayserili H, Al-Gazali L, Shahwan M, Brancati F, Lee H, O'Connor BD, Schmidt-von Kegler M, Merriman B, Nelson SF, Masri A, Alkazaleh F, Guerra D, Ferrari P, Nanda A, Rajab A, Markie D, Gray M, Nelson J, Grix A, Sommer A, Savarirayan R, Janecke AR, Steichen E, Sillence D, Hausser I, Budde B, N?rnberg G, N?rnberg P, Seemann P, Kunkel D, Zambruno G, Dallapiccola B, Schuelke M, Robertson S, Hamamy H, Wollnik B, Van Maldergem L, Mundlos S, Kornak U
Nature genetics41(9)1016-21(2009 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following pycr1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pycr1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa02315	NM_200826.1 Latest version!	Danio rerio pyrroline-5-carboxylate reductase 1a (pycr1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
ODa08885	XM_021469847.1 Latest version!	Danio rerio pyrroline-5-carboxylate reductase 1a (pycr1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pycr1a ( NM_200826.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_957120.1 Danio rerio pyrroline-5-carboxylate reductase 1a (pycr1a), mRNA.

Price & Availability↑

CloneID	ODa02315
Clone ID Related Accession (Same CDS sequence)	NM_200826.1
Accession Version	NM_200826.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Danio rerio(zebrafish)
Product	pyrroline-5-carboxylate reductase 1, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC060905.1. On Aug 31, 2013 this sequence version replaced XM_005171640.1. ##Evidence-Data-START## Transcript exon combination :: BC060905.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGAGTGTGG GATTTATCGG AGCTGGTCAG CTGGCGCATG CGCTGGTCAA GGGCTTCACA 
GCCGCAGGTG TGATCGCCAC ACACAGGATA ACAGCGAGTT CTCCAGACAC TGACCTGCCG 
ACGGTTATTG GCCTGAGAAA AATGGGAGCG TTCTTCACCA CCAGTAATAA AGAGACCGTG 
AGCAAGAGTG ACGTCCTTTT TCTGGCGGTC AAGCCTCATA TCATTCCCTT TGTCCTGGAC 
GAGATCGGGC CAGACATTGA AGACCGCCAC CTCATAGTCT CCTGTGCTGC AGGAGTGACC 
ATCAGCTCCA TCGAGAAGAA ACTGCTGCAG TACCGTTCAG CACCTAAAGT CATTCGCTGC 
ATGACAAACA CCCCGGTGGT GGTCCGCGAG GGGGCCACGG TTTACGCCAC AGGCACCCAT 
GCGGAGGTGG AGGACGGGAA GCTCCTGGAG CAGCTAATGG CCAGTGTGGG CTACTGCACC 
GAGGTGGAAG AGGACCTGAT TGATGCCGTC ACTGGCCTCA GTGGCAGTGG ACCAGCCTAT 
GCATTCACAG CGGTTGATGC TCTTGCTGAT GGAGGGGTCA AGATGGGATT GCCCAGAAGA 
CTGGCTGTAC GACTGGGAGC TCAGGCCCTG CTTGGAGCTG CAAAAATGCT CCTTGAGTCA 
GAGCAACACC CTGGACAGTT AAAGGACAAC GTGGCTTCGC CTGGAGGTGC TACAATCCAT 
GCTTTGCATG TGATGGAAAG TGGCGGTTTC CGCAGCCTCC TGATCAATGC TGTGGAGGCT 
TCGTGCATTA GAACCAGGGA GCTTCAGTAT TTGGCAGATC AAGAGAAGAT CTCTCCCGCT 
GCCATCAAGA AGACGACTCT GGACAAAGTG CTCCAACAGC CAGGGGTCAC TGCAGCAGGG 
GTAGGGGTCC GGACAGGAAT CAACCTCTTT AACAGTACAA ACCCCAAACA CAAGAAAAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_957120.1
CDS	99..1061
Translation	MSVGFIGAGQLAHALVKGFTAAGVIATHRITASSPDTDLPTVIGLRKMGAFFTTSNKETVSKSDVLFLAVKPHIIPFVLDEIGPDIEDRHLIVSCAAGVTISSIEKKLLQYRSAPKVIRCMTNTPVVVREGATVYATGTHAEVEDGKLLEQLMASVGYCTEVEEDLIDAVTGLSGSGPAYAFTAVDALADGGVKMGLPRRLAVRLGAQALLGAAKMLLESEQHPGQLKDNVASPGGATIHALHVMESGGFRSLLINAVEASCIRTRELQYLADQEKISPAAIKKTTLDKVLQQPGVTAAGVGVRTGINLFNSTNPKHKKS

Target ORF information:

RefSeq Version	NM_200826.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio pyrroline-5-carboxylate reductase 1a (pycr1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200826.1

ORF Insert Sequence:

ATGAGTGTGG GATTTATCGG AGCTGGTCAG CTGGCGCATG CGCTGGTCAA GGGCTTCACA 
GCCGCAGGTG TGATCGCCAC ACACAGGATA ACAGCGAGTT CTCCAGACAC TGACCTGCCG 
ACGGTTATTG GCCTGAGAAA AATGGGAGCG TTCTTCACCA CCAGTAATAA AGAGACCGTG 
AGCAAGAGTG ACGTCCTTTT TCTGGCGGTC AAGCCTCATA TCATTCCCTT TGTCCTGGAC 
GAGATCGGGC CAGACATTGA AGACCGCCAC CTCATAGTCT CCTGTGCTGC AGGAGTGACC 
ATCAGCTCCA TCGAGAAGAA ACTGCTGCAG TACCGTTCAG CACCTAAAGT CATTCGCTGC 
ATGACAAACA CCCCGGTGGT GGTCCGCGAG GGGGCCACGG TTTACGCCAC AGGCACCCAT 
GCGGAGGTGG AGGACGGGAA GCTCCTGGAG CAGCTAATGG CCAGTGTGGG CTACTGCACC 
GAGGTGGAAG AGGACCTGAT TGATGCCGTC ACTGGCCTCA GTGGCAGTGG ACCAGCCTAT 
GCATTCACAG CGGTTGATGC TCTTGCTGAT GGAGGGGTCA AGATGGGATT GCCCAGAAGA 
CTGGCTGTAC GACTGGGAGC TCAGGCCCTG CTTGGAGCTG CAAAAATGCT CCTTGAGTCA 
GAGCAACACC CTGGACAGTT AAAGGACAAC GTGGCTTCGC CTGGAGGTGC TACAATCCAT 
GCTTTGCATG TGATGGAAAG TGGCGGTTTC CGCAGCCTCC TGATCAATGC TGTGGAGGCT 
TCGTGCATTA GAACCAGGGA GCTTCAGTAT TTGGCAGATC AAGAGAAGAT CTCTCCCGCT 
GCCATCAAGA AGACGACTCT GGACAAAGTG CTCCAACAGC CAGGGGTCAC TGCAGCAGGG 
GTAGGGGTCC GGACAGGAAT CAACCTCTTT AACAGTACAA ACCCCAAACA CAAGAAAAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pycr1a ( XM_021469847.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021325522.1 Danio rerio pyrroline-5-carboxylate reductase 1a (pycr1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa08885
Clone ID Related Accession (Same CDS sequence)	XM_021469847.1
Accession Version	XM_021469847.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	pyrroline-5-carboxylate reductase 1, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGG GATTTATCGG AGCTGGTCAG CTGGCGCATG CGCTGGTCAA GGGCTTCACA 
GCCGCAGGTG TGATCGCCAC ACACAGGATA ACAGCGAGTT CTCCAGACAC TGACCTGCCG 
ACGGTCATTG GCCTGAGAAA AATGGGAGCG TTCTTCACCA CCAGTAATAA AGAGACCGTG 
AGCAAGAGTG ACGTCCTTTT CCTGGCGGTC AAGCCTCATA TCATTCCCTT TGTCCTGGAC 
GAGATCGGCC CAGACATTGA AGACCGCCAC CTCATCGTCT CCTGTGCTGC AGGAGTGACC 
ATCAGCTCCA TCGAGAAGAA ACTGCTGCAG TACCGTTCAG CACCTAAAGT CATTCGCTGC 
ATGACAAACA CCCCGGTGGT GGTCCGCGAG GGGGCCACGG TTTACGCCAC AGGCACCCAT 
GCGGAGGTGG AGGACGGGAA GCTCCTGGAG CAGCTAATGG CCAGTGTGGG CTACTGCACC 
GAGGTGGAAG AGGACCTGAT TGATGCCGTC ACTGGCCTCA GTGGCAGTGG ACCAGCCTAT 
GCATTCACAG CGGTTGATGC TCTTGCTGAT GGAGGGGTGA AGATGGGATT GCCCAGAAGA 
CTGGCTGTAC GACTGGGAGC TCAGGCCCTG CTTGGAGCGG CAAAAATGCT CCTTGAGTCA 
GAGCAACACC CTGGACAGTT AAAGGACAAC GTGGCTTCGC CTGGAGGTGC TACAATCCAC 
GCTTTGCATG TGATGGAAAG TGGCGGTTTC CGCAGCCTCC TGATCAATGC TGTGGAGGCT 
TCGTGCATTA GAACCAGGGA GCTTCAGTAT TTGGCAGATC AAGAGAAGAT CTCTCCCGCT 
GCCATCAAGA AGACGACTCT GGACAAAGTG CTCCAGCAGC CGGGGGTGAC AGCAGCAGGG 
GTCGGGGTCC GGACAGGAAT CAACCTCTTT AACAGTACAA ACCCCAAACA CAAGAAAAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325522.1
CDS	137..1099
Translation	MSVGFIGAGQLAHALVKGFTAAGVIATHRITASSPDTDLPTVIGLRKMGAFFTTSNKETVSKSDVLFLAVKPHIIPFVLDEIGPDIEDRHLIVSCAAGVTISSIEKKLLQYRSAPKVIRCMTNTPVVVREGATVYATGTHAEVEDGKLLEQLMASVGYCTEVEEDLIDAVTGLSGSGPAYAFTAVDALADGGVKMGLPRRLAVRLGAQALLGAAKMLLESEQHPGQLKDNVASPGGATIHALHVMESGGFRSLLINAVEASCIRTRELQYLADQEKISPAAIKKTTLDKVLQQPGVTAAGVGVRTGINLFNSTNPKHKKS

Target ORF information:

RefSeq Version	XM_021469847.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio pyrroline-5-carboxylate reductase 1a (pycr1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469847.1

ORF Insert Sequence:

ATGAGTGTGG GATTTATCGG AGCTGGTCAG CTGGCGCATG CGCTGGTCAA GGGCTTCACA 
GCCGCAGGTG TGATCGCCAC ACACAGGATA ACAGCGAGTT CTCCAGACAC TGACCTGCCG 
ACGGTCATTG GCCTGAGAAA AATGGGAGCG TTCTTCACCA CCAGTAATAA AGAGACCGTG 
AGCAAGAGTG ACGTCCTTTT CCTGGCGGTC AAGCCTCATA TCATTCCCTT TGTCCTGGAC 
GAGATCGGCC CAGACATTGA AGACCGCCAC CTCATCGTCT CCTGTGCTGC AGGAGTGACC 
ATCAGCTCCA TCGAGAAGAA ACTGCTGCAG TACCGTTCAG CACCTAAAGT CATTCGCTGC 
ATGACAAACA CCCCGGTGGT GGTCCGCGAG GGGGCCACGG TTTACGCCAC AGGCACCCAT 
GCGGAGGTGG AGGACGGGAA GCTCCTGGAG CAGCTAATGG CCAGTGTGGG CTACTGCACC 
GAGGTGGAAG AGGACCTGAT TGATGCCGTC ACTGGCCTCA GTGGCAGTGG ACCAGCCTAT 
GCATTCACAG CGGTTGATGC TCTTGCTGAT GGAGGGGTGA AGATGGGATT GCCCAGAAGA 
CTGGCTGTAC GACTGGGAGC TCAGGCCCTG CTTGGAGCGG CAAAAATGCT CCTTGAGTCA 
GAGCAACACC CTGGACAGTT AAAGGACAAC GTGGCTTCGC CTGGAGGTGC TACAATCCAC 
GCTTTGCATG TGATGGAAAG TGGCGGTTTC CGCAGCCTCC TGATCAATGC TGTGGAGGCT 
TCGTGCATTA GAACCAGGGA GCTTCAGTAT TTGGCAGATC AAGAGAAGAT CTCTCCCGCT 
GCCATCAAGA AGACGACTCT GGACAAAGTG CTCCAGCAGC CGGGGGTGAC AGCAGCAGGG 
GTCGGGGTCC GGACAGGAAT CAACCTCTTT AACAGTACAA ACCCCAAACA CAAGAAAAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A rapid and effective method for screening, sequencing and reporter verification of engineered frameshift mutations in zebrafish.
Disease models & mechanisms10(6)811-822(2017 Jun)
Prykhozhij SV,Steele SL,Razaghi B,Berman JN

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Mutations in PYCR2, Encoding Pyrroline-5-Carboxylate Reductase 2, Cause Microcephaly and Hypomyelination.
American journal of human genetics96(5)709-19(2015 May)
Nakayama T,Al-Maawali A,El-Quessny M,Rajab A,Khalil S,Stoler JM,Tan WH,Nasir R,Schmitz-Abe K,Hill RS,Partlow JN,Al-Saffar M,Servattalab S,LaCoursiere CM,Tambunan DE,Coulter ME,Elhosary PC,Gorski G,Barkovich AJ,Markianos K,Poduri A,Mochida GH

Mutations in PYCR1 cause cutis laxa with progeroid features.
Nature genetics41(9)1016-21(2009 Sep)
Reversade B,Escande-Beillard N,Dimopoulou A,Fischer B,Chng SC,Li Y,Shboul M,Tham PY,Kayserili H,Al-Gazali L,Shahwan M,Brancati F,Lee H,O'Connor BD,Schmidt-von Kegler M,Merriman B,Nelson SF,Masri A,Alkazaleh F,Guerra D,Ferrari P,Nanda A,Rajab A,Markie D,Gray M,Nelson J,Grix A,Sommer A,Savarirayan R,Janecke AR,Steichen E,Sillence D,Hausser I,Budde B,N?rnberg G,N?rnberg P,Seemann P,Kunkel D,Zambruno G,Dallapiccola B,Schuelke M,Robertson S,Hamamy H,Wollnik B,Van Maldergem L,Mundlos S,Kornak U

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pycr1a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム