aprt cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	aprt
Entrez Gene ID	393641
Full Name	adenine phosphoribosyltransferase
Synonyms	zgc:65965
General protein information	Preferred Names adenine phosphoribosyltransferase Names adenine phosphoribosyltransferase
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_200668.1	NP_956962.1	adenine phosphoribosyltransferase

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_AER325W	NP_985181.1
Magnaporthe oryzae (rice blast fungus)	MGG_17399	XP_003718359.1
Canis lupus familiaris (dog)	APRT	XP_536752.2
Mus musculus (house mouse)	Aprt	NP_033828.2
Danio rerio (zebrafish)	aprt	NP_956962.1
Saccharomyces cerevisiae (baker's yeast)	APT1	NP_013690.1
Neurospora crassa	NCU02090	XP_963727.2
Xenopus tropicalis (tropical clawed frog)	aprt	NP_001007941.1
Homo sapiens (human)	APRT	NP_000476.1
	APRT	XP_001089867.1
Bos taurus (cattle)	APRT	NP_001020505.1
Gallus gallus (chicken)	APRT	NP_001264576.1
Drosophila melanogaster (fruit fly)	Aprt	NP_476637.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005723	XP_315739.4
Kluyveromyces lactis	KLLA0B01309g	XP_451589.1
Schizosaccharomyces pombe (fission yeast)	apt1	NP_594433.1
Oryza sativa (rice)	Os12g0589100	NP_001067164.1
Pan troglodytes (chimpanzee)	APRT	XP_003952966.1
Rattus norvegicus (Norway rat)	Aprt	NP_001013079.1
Caenorhabditis elegans (roundworm)	T19B4.3	NP_491663.1
Arabidopsis thaliana (thale cress)	APT1	NP_564284.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Base excision repair in early zebrafish development: evidence for DNA polymerase switching and standby AP endonuclease activity.
Fortier S, Yang X, Wang Y, Bennett RA, Strauss PR
Biochemistry48(23)5396-404(2009 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following aprt gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the aprt cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa03821	NM_200668.1 Latest version!	Danio rerio adenine phosphoribosyltransferase (aprt), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

aprt ( NM_200668.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956962.1 Danio rerio adenine phosphoribosyltransferase (aprt), mRNA.

Price & Availability↑

CloneID	ODa03821
Clone ID Related Accession (Same CDS sequence)	NM_200668.1
Accession Version	NM_200668.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 534bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	adenine phosphoribosyltransferase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC058046.1. ##Evidence-Data-START## Transcript exon combination :: BC058046.1, EH499598.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCAGATA AACTGGAATT AATCTCCAAG TGCATAAGAA CTTTCCCTGA TTTCCCTACG 
AAAGGCATTC CGTTCAAAGA TATTTTCCCA ATCTTAAAGG ACCCGAAGGC TGTTACTGCA 
GTGACAGATC TGTTTGAAGA GCACGTGAGA AACACTTACC CTCAGGTGGA TCTCATAGTG 
GGACTTGATG CTCGAGGGTT TCTATTCGGG CCACTTTTGG CTTTGAGATT AGGAATAGGA 
TTCGCTCCGA TCAGGAAAAA AGGAAAGCTC CCTGGTCCCA CAATATCAGT TGCCTATAGT 
CTTGAGTATG CAAAGGCCGA GGCAGAGATG CAGGAAGATG CTGTATCTGC TGGACAGAAA 
GTGTTAATCA TTGATGATTT ACTTGCTACA GGAGGCACTC TGTATGCAGC CATTGAGCTG 
ATCAAGCAGC AGAAGGCAGA GGTTTTAGGC TGTTTGGTGG TGGTGGAGCT CAAGTATCTC 
AATGGATCTG ACAAGCTGAA GCCAACACCG GTGTTCTCTC TAATACAGTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956962.1
CDS	22..555
Translation	MADKLELISKCIRTFPDFPTKGIPFKDIFPILKDPKAVTAVTDLFEEHVRNTYPQVDLIVGLDARGFLFGPLLALRLGIGFAPIRKKGKLPGPTISVAYSLEYAKAEAEMQEDAVSAGQKVLIIDDLLATGGTLYAAIELIKQQKAEVLGCLVVVELKYLNGSDKLKPTPVFSLIQY

Target ORF information:

RefSeq Version	NM_200668.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio adenine phosphoribosyltransferase (aprt), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200668.1

ORF Insert Sequence:

ATGGCAGATA AACTGGAATT AATCTCCAAG TGCATAAGAA CTTTCCCTGA TTTCCCTACG 
AAAGGCATTC CGTTCAAAGA TATTTTCCCA ATCTTAAAGG ACCCGAAGGC TGTTACTGCA 
GTGACAGATC TGTTTGAAGA GCACGTGAGA AACACTTACC CTCAGGTGGA TCTCATAGTG 
GGACTTGATG CTCGAGGGTT TCTATTCGGG CCACTTTTGG CTTTGAGATT AGGAATAGGA 
TTCGCTCCGA TCAGGAAAAA AGGAAAGCTC CCTGGTCCCA CAATATCAGT TGCCTATAGT 
CTTGAGTATG CAAAGGCCGA GGCAGAGATG CAGGAAGATG CTGTATCTGC TGGACAGAAA 
GTGTTAATCA TTGATGATTT ACTTGCTACA GGAGGCACTC TGTATGCAGC CATTGAGCTG 
ATCAAGCAGC AGAAGGCAGA GGTTTTAGGC TGTTTGGTGG TGGTGGAGCT CAAGTATCTC 
AATGGATCTG ACAAGCTGAA GCCAACACCG GTGTTCTCTC TAATACAGTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Base excision repair in early zebrafish development: evidence for DNA polymerase switching and standby AP endonuclease activity.
Biochemistry48(23)5396-404(2009 Jun)
Fortier S,Yang X,Wang Y,Bennett RA,Strauss PR