med27 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	med27
Entrez Gene ID	393633
Full Name	mediator complex subunit 27
Synonyms	crsp8,zgc:66302
General protein information	Preferred Names mediator complex subunit 27 Names mediator of RNA polymerase II transcription subunit 27 CRSP complex subunit 8 cofactor required for Sp1 transcriptional activation, subunit 8, 34kDa crsp34 trap37
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021478216.1	XP_021333891.1	mediator of RNA polymerase II transcription subunit 27 isoform X1
NM_200660.1	NP_956954.1	mediator of RNA polymerase II transcription subunit 27

Pathways from BioSystems

Homologies

	MED27	XP_001105126.1
Danio rerio (zebrafish)	med27	NP_956954.1
Mus musculus (house mouse)	Med27	NP_081172.2
Anopheles gambiae (African malaria mosquito)	MED27_ANOGA	XP_312260.3
Homo sapiens (human)	MED27	NP_004260.2
Rattus norvegicus (Norway rat)	Med27	NP_001100035.1
Gallus gallus (chicken)	MED27	NP_001264204.1
Drosophila melanogaster (fruit fly)	MED27	NP_649569.1
Bos taurus (cattle)	MED27	NP_001035636.1
Canis lupus familiaris (dog)	MED27	XP_849826.1
Xenopus tropicalis (tropical clawed frog)	med27	XP_004916727.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Differential roles of transcriptional mediator complex subunits Crsp34/Med27, Crsp150/Med14 and Trap100/Med24 during zebrafish retinal development.
D?rr K, Holzschuh J, Filippi A, Ettl AK, Ryu S, Shepherd IT, Driever W
Genetics174(2)693-705(2006 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ubiquitous expression of crsp34 mRNA may suggest a universal requirement for Crsp34 in transcriptional initiation.
Title: Differential roles of transcriptional mediator complex subunits Crsp34/Med27, Crsp150/Med14 and Trap100/Med24 during zebrafish retinal development.

The following med27 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the med27 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa37873	NM_200660.1 Latest version!	Danio rerio mediator complex subunit 27 (med27), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
ODa143667	XM_021478216.1 Latest version!	Danio rerio mediator complex subunit 27 (med27), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

med27 ( NM_200660.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956954.1 Danio rerio mediator complex subunit 27 (med27), mRNA.

Price & Availability↑

CloneID	ODa37873
Clone ID Related Accession (Same CDS sequence)	NM_200660.1
Accession Version	NM_200660.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	mediator of RNA polymerase II transcription subunit 27
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC057508.1. ##Evidence-Data-START## Transcript exon combination :: BC057508.1, EH580031.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGATG TTATGAATGT TGGAGTGAAT TTGGAAGCGT TTTCTCAAGC GATAAACGCC 
ATCCAGGCTC TTCGGTCGAG CGTCACAAGA GTTTTCGACT TTCTTAAGGA TGGGATGAAG 
AATAAGGAGA CCCTTGAAAG CCGGGAGAAA GTCTTTATAA CTGAGTTTCA AGAGAATCTG 
CAGTCAGTGA ATCGCGATTT AAATGAGTTG GAGCGTCTCA GCACTTTGGT TGGCAGGCCG 
TCAGAGAGTC ACCCCTTACA CAACAGTGGA CTGCTCAGTT TAGACCCTGT GCAGGACAAA 
ACCCCTCTGT ACAGCCAGCT GCTCCAGGCC TACAAATGGT CCAATAAATT GCAGTATCAT 
GCAGGTCTGG CCTCCAGTCT TCTGAACCAA CAGTCACTCA AACGCTCAGC CAATCAGATG 
GGAACATCTG CCAAACGAAG ACCCAAAGTC CAGCCAAGCA CCCTCGCCCT TCCAACACAA 
TATGTGGATG ATGTCATATC CAGAATTGGC CGAATGTTTC CAGACATGTC AATCGAGCTT 
TTCCGACCGA ACGGGACATC AGCGGTTCTA TTGGTGACTC TGGGGAAGGT TTTGAAAGCC 
ATCGTGGTAA TGAGGAGCCT TTTCATCGAC CGCACGATTG TCAGAGGTTT CCACGAGAAC 
ATATACACAG AGGACCGAAA GCTGGACATC TGGTCGAAGT CCAACTATCA AGCATTTCAA 
AAGGTCACTG ATCATGCAAC CACAGCCCTC CTGCATTACC AGCTACCGCA GATGCCTGAT 
GTTGTGGTTC GCTCCTTCAT GACCTGGTTG AGGAGCTACA TCAAGCTTTT CCAGACCCCG 
TGTCAGCGCT GTGGCAAATA CCTGCAGGAG GGTCTTCCTC CTACATGGAG GGACTTCCGA 
ACTCTGGAGG CTTTCCATGA CACCTGCCGG CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956954.1
CDS	22..957
Translation	MADVMNVGVNLEAFSQAINAIQALRSSVTRVFDFLKDGMKNKETLESREKVFITEFQENLQSVNRDLNELERLSTLVGRPSESHPLHNSGLLSLDPVQDKTPLYSQLLQAYKWSNKLQYHAGLASSLLNQQSLKRSANQMGTSAKRRPKVQPSTLALPTQYVDDVISRIGRMFPDMSIELFRPNGTSAVLLVTLGKVLKAIVVMRSLFIDRTIVRGFHENIYTEDRKLDIWSKSNYQAFQKVTDHATTALLHYQLPQMPDVVVRSFMTWLRSYIKLFQTPCQRCGKYLQEGLPPTWRDFRTLEAFHDTCRQ

Target ORF information:

RefSeq Version	NM_200660.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mediator complex subunit 27 (med27), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200660.1

ORF Insert Sequence:

ATGGCGGATG TTATGAATGT TGGAGTGAAT TTGGAAGCGT TTTCTCAAGC GATAAACGCC 
ATCCAGGCTC TTCGGTCGAG CGTCACAAGA GTTTTCGACT TTCTTAAGGA TGGGATGAAG 
AATAAGGAGA CCCTTGAAAG CCGGGAGAAA GTCTTTATAA CTGAGTTTCA AGAGAATCTG 
CAGTCAGTGA ATCGCGATTT AAATGAGTTG GAGCGTCTCA GCACTTTGGT TGGCAGGCCG 
TCAGAGAGTC ACCCCTTACA CAACAGTGGA CTGCTCAGTT TAGACCCTGT GCAGGACAAA 
ACCCCTCTGT ACAGCCAGCT GCTCCAGGCC TACAAATGGT CCAATAAATT GCAGTATCAT 
GCAGGTCTGG CCTCCAGTCT TCTGAACCAA CAGTCACTCA AACGCTCAGC CAATCAGATG 
GGAACATCTG CCAAACGAAG ACCCAAAGTC CAGCCAAGCA CCCTCGCCCT TCCAACACAA 
TATGTGGATG ATGTCATATC CAGAATTGGC CGAATGTTTC CAGACATGTC AATCGAGCTT 
TTCCGACCGA ACGGGACATC AGCGGTTCTA TTGGTGACTC TGGGGAAGGT TTTGAAAGCC 
ATCGTGGTAA TGAGGAGCCT TTTCATCGAC CGCACGATTG TCAGAGGTTT CCACGAGAAC 
ATATACACAG AGGACCGAAA GCTGGACATC TGGTCGAAGT CCAACTATCA AGCATTTCAA 
AAGGTCACTG ATCATGCAAC CACAGCCCTC CTGCATTACC AGCTACCGCA GATGCCTGAT 
GTTGTGGTTC GCTCCTTCAT GACCTGGTTG AGGAGCTACA TCAAGCTTTT CCAGACCCCG 
TGTCAGCGCT GTGGCAAATA CCTGCAGGAG GGTCTTCCTC CTACATGGAG GGACTTCCGA 
ACTCTGGAGG CTTTCCATGA CACCTGCCGG CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

med27 ( XM_021478216.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333891.1 Danio rerio mediator complex subunit 27 (med27), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa143667
Clone ID Related Accession (Same CDS sequence)	XM_021478216.1
Accession Version	XM_021478216.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	mediator of RNA polymerase II transcription subunit 27 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATG TTATGAATGT TGGAGTGAAT TTGGAAGCGT TTTCTCAAGC GATAAACGCC 
ATCCAGGCTC TTCGGTCGAG CGTCACAAGA GTTTTCGACT TTCTCAAGGA TGGGATGAAG 
AATAAGGAGA CCCTTGAAAG CCGGGAGAAA GTCTTTATAA CTGAGTTTCA AGAGAATCTG 
CAGTCAGTGA ATCGCGATTT AAATGAGTTG GAGCGTCTCA GCACTTTGGT TGGCAGGCCG 
TCAGAGAGTC ACCCCTTACA CAACAGTGGA CTGCTCAGTT TAGACCCTGT GCAGGACAAA 
ACCCCTCTGT ACAGCCAGCT GCTCCAGGCT TACAAATGGT CCAATAAATT GCAGTATCAT 
GCAGGTCTGG CCTCCAGTCT TCTGAACCAA CAGTCACTCA AACGCTCAGC CAATCAGATG 
GGAACATCTG CCAAACGAAG ACCCAAAGTC CAGCCAAGCA CCCTCGCCCT TCCAACACAA 
TATGTGGATG ATGTCATATC CAGAATTGGC CGAATGTTTC CAGACATGTC AATCGAGCTT 
TTCCGACCGA ACGGGACATC AGCGGTTCTA TTGGTGACTC TGGGGAAGGT TTTGAAAGCC 
ATCGTGGTAA TGAGGAGCCT TTTCATCGAC CGCACGATTG TCAGAGGTTT CCACGAGAAC 
ATATACACAG AGGACCGAAA GAAAGCTGCA ACTGACTGTC ACAGCAACGC TCGCACCGGG 
ATATCAGCAG TGACGACCAA ACTGAGCTTA TTACTGCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333891.1
CDS	42..803
Translation	MADVMNVGVNLEAFSQAINAIQALRSSVTRVFDFLKDGMKNKETLESREKVFITEFQENLQSVNRDLNELERLSTLVGRPSESHPLHNSGLLSLDPVQDKTPLYSQLLQAYKWSNKLQYHAGLASSLLNQQSLKRSANQMGTSAKRRPKVQPSTLALPTQYVDDVISRIGRMFPDMSIELFRPNGTSAVLLVTLGKVLKAIVVMRSLFIDRTIVRGFHENIYTEDRKKAATDCHSNARTGISAVTTKLSLLLR

Target ORF information:

RefSeq Version	XM_021478216.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mediator complex subunit 27 (med27), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478216.1

ORF Insert Sequence:

ATGGCGGATG TTATGAATGT TGGAGTGAAT TTGGAAGCGT TTTCTCAAGC GATAAACGCC 
ATCCAGGCTC TTCGGTCGAG CGTCACAAGA GTTTTCGACT TTCTCAAGGA TGGGATGAAG 
AATAAGGAGA CCCTTGAAAG CCGGGAGAAA GTCTTTATAA CTGAGTTTCA AGAGAATCTG 
CAGTCAGTGA ATCGCGATTT AAATGAGTTG GAGCGTCTCA GCACTTTGGT TGGCAGGCCG 
TCAGAGAGTC ACCCCTTACA CAACAGTGGA CTGCTCAGTT TAGACCCTGT GCAGGACAAA 
ACCCCTCTGT ACAGCCAGCT GCTCCAGGCT TACAAATGGT CCAATAAATT GCAGTATCAT 
GCAGGTCTGG CCTCCAGTCT TCTGAACCAA CAGTCACTCA AACGCTCAGC CAATCAGATG 
GGAACATCTG CCAAACGAAG ACCCAAAGTC CAGCCAAGCA CCCTCGCCCT TCCAACACAA 
TATGTGGATG ATGTCATATC CAGAATTGGC CGAATGTTTC CAGACATGTC AATCGAGCTT 
TTCCGACCGA ACGGGACATC AGCGGTTCTA TTGGTGACTC TGGGGAAGGT TTTGAAAGCC 
ATCGTGGTAA TGAGGAGCCT TTTCATCGAC CGCACGATTG TCAGAGGTTT CCACGAGAAC 
ATATACACAG AGGACCGAAA GAAAGCTGCA ACTGACTGTC ACAGCAACGC TCGCACCGGG 
ATATCAGCAG TGACGACCAA ACTGAGCTTA TTACTGCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Differential roles of transcriptional mediator complex subunits Crsp34/Med27, Crsp150/Med14 and Trap100/Med24 during zebrafish retinal development.
Genetics174(2)693-705(2006 Oct)
D?rr K,Holzschuh J,Filippi A,Ettl AK,Ryu S,Shepherd IT,Driever W