ilk cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ilk
Entrez Gene ID	393543
Full Name	integrin-linked kinase
Synonyms	zgc:65842
General protein information	Preferred Names integrin-linked kinase Names integrin-linked protein kinase loc lost-contact main squeeze msq zilk
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_200571.1	NP_956865.1	integrin-linked protein kinase

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ILK	XP_534040.1
Rattus norvegicus (Norway rat)	Ilk	NP_596900.1
	ILK	XP_001109041.1
Gallus gallus (chicken)	ILK	NP_989525.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006573	XP_316598.2
Pan troglodytes (chimpanzee)	ILK	XP_003312944.1
Mus musculus (house mouse)	Ilk	NP_001155196.1
Xenopus tropicalis (tropical clawed frog)	ilk	NP_001120512.1
Danio rerio (zebrafish)	ilk	NP_956865.1
Homo sapiens (human)	ILK	NP_004508.1
Bos taurus (cattle)	ILK	NP_001029865.1
Drosophila melanogaster (fruit fly)	Ilk	NP_525001.2
Caenorhabditis elegans (roundworm)	pat-4	NP_497139.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

A mutation in the atrial-specific myosin light chain gene (MYL4) causes familial atrial fibrillation.
Orr N, Arnaout R, Gula LJ, Spears DA, Leong-Sit P, Li Q, Tarhuni W, Reischauer S, Chauhan VS, Borkovich M, Uppal S, Adler A, Coughlin SR, Stainier DY, Gollob MH
Nature communications711303(2016 Apr)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that integrin-linked kinase is an essential component downstream of laminin and integrin alpha7, providing strengthening of skeletal muscle fibre adhesion with the extracellular matrix.
Title: Zebrafish integrin-linked kinase is required in skeletal muscles for strengthening the integrin-ECM adhesion complex.

The lost-contact mutant on chromosome 10 encodes a nonsense mutation (Y319X) associated with defective cardiomyocytes & endothelial cells that leads to severe myocardial dysfunction. There is epistatic regulation between laminin-alpha4, integrin, & Ilk.
Title: Laminin-alpha4 and integrin-linked kinase mutations cause human cardiomyopathy via simultaneous defects in cardiomyocytes and endothelial cells.

Heart failure in recessive embryonic lethal zebrafish mutant main squeeze (msq) mutants is due to a mutation in the integrin-linked kinase (ilk) gene.
Title: Integrin-linked kinase, a novel component of the cardiac mechanical stretch sensor, controls contractility in the zebrafish heart.

The following ilk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ilk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa02733	NM_200571.1 Latest version!	Danio rerio integrin-linked kinase (ilk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ilk ( NM_200571.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956865.1 Danio rerio integrin-linked kinase (ilk), mRNA.

Price & Availability↑

CloneID	ODa02733
Clone ID Related Accession (Same CDS sequence)	NM_200571.1
Accession Version	NM_200571.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Danio rerio(zebrafish)
Product	integrin-linked protein kinase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC056593.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC056593.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGATGACA TCTTCACTCA GTGCCGTGAA GGCAACGCGG TAGCCGTTCG CCTCTGGCTG 
GACAACACAG AGAATGACCT AAACCAGGGG GATGATCACG GCTTCAGTCC GTTGCACTGG 
GCGTGCCGCG AGGGCCGTTC GGGTGTTGTG GATATGCTCA TCATGAGAGG TGCTCGTATC 
AATGTGATGA ACCGCGGCGA CGACACACCC CTGCACCTGG CAGCGAGCCA TGGCCATCGT 
GACATTCTGG CCAAGCTGAT TCAGTGTAAA GCAGATACCA ATGCGGCAAA CGAGCACGGA 
AACACCCCTC TACATTATGC CTGCTTCTGG GCCCACGATC AGGTGGCGGA GGATCTGGTC 
AGTAATGGAG CTCAGGTGAG CATCTGCAAC AAGTATGGAG AAACTCCAAT GGACAAGGCC 
AAACCACCGC TTGCAGCAAA TCTAAAAGAC CTGGCAGAGA AACAAGGACA GAGCCTGTCA 
AAAGTCCCTT ACAAGGACAA TTTCTGGAAA GGAACAACTC GAACACGACC ACGTAATGGC 
ACACTAAACA AGCAAGCTGG CATAGATTAT AAGCAGCTTA ACATGCTGAA CAAGATCAAC 
GAGAACCACT CCGGCGAGCT GTGGAAGGGC CGCTGGCAGG GCACAGAGAT TGTGGTCAAG 
ATGCTGCATG TGCGGGAATG GACCACCAGA AAGAGCCGAG ACTTTAATGA GGAGTACCCT 
AAACTAAGAA TCTTCTCTCA CCCAAACGTG TTGCCCGTGT TGGGGGCGTG TCAGTCTCCA 
CCTGCCCCTC ACCCAATCAT CATCACACAC TCTATGCCCT ACGGTTCCCT CTACAACTTG 
CTGCATGAGG GCACCAACTT AGTAGTGGAT CAAAACCAGG CTGTGAAATT TGCTCTGGAC 
ATTGCCTGTG GAATGGCTTT CCTACACACT CTTGAGCCCA TGATCCCCCG CCATTATCTC 
AACAGCAAGA GTGTCATGAT TGACGAGGAC ATGACGGCCA GGATAAGCAT GGCAGATGTA 
AAGTTGTCAT TTCAGTGTCC TGGCAGGATG TACTCTCCTG CATGGGTGGC ACCTGAAGCT 
CTGCAGAAGA AGCCAGAGGA GATCAACCGG CGCTCAGCTG ACATGTGGAG CTACGCCGTG 
CTGCTTTGGG AGCTGGTGAG CAGAGAAGTG CCGTTTGCTG ACCTCTCAAA CATGGAGATC 
GGCATGAAGG TGGCGCTAGA GGGACTACGG CCCACCATCC CACCCGGCAT CTCCCCTCAC 
ATCTGCAAAC TCATGAAGAT CTGCATGAAC GAAGACCCTG CAAAGAGGCC CAAATTCGAC 
ATGATCGTGC CCATCCTGGA GAAGATGCAA GACAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956865.1
CDS	239..1597
Translation	MDDIFTQCREGNAVAVRLWLDNTENDLNQGDDHGFSPLHWACREGRSGVVDMLIMRGARINVMNRGDDTPLHLAASHGHRDILAKLIQCKADTNAANEHGNTPLHYACFWAHDQVAEDLVSNGAQVSICNKYGETPMDKAKPPLAANLKDLAEKQGQSLSKVPYKDNFWKGTTRTRPRNGTLNKQAGIDYKQLNMLNKINENHSGELWKGRWQGTEIVVKMLHVREWTTRKSRDFNEEYPKLRIFSHPNVLPVLGACQSPPAPHPIIITHSMPYGSLYNLLHEGTNLVVDQNQAVKFALDIACGMAFLHTLEPMIPRHYLNSKSVMIDEDMTARISMADVKLSFQCPGRMYSPAWVAPEALQKKPEEINRRSADMWSYAVLLWELVSREVPFADLSNMEIGMKVALEGLRPTIPPGISPHICKLMKICMNEDPAKRPKFDMIVPILEKMQDK

Target ORF information:

RefSeq Version	NM_200571.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio integrin-linked kinase (ilk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200571.1

ORF Insert Sequence:

ATGGATGACA TCTTCACTCA GTGCCGTGAA GGCAACGCGG TAGCCGTTCG CCTCTGGCTG 
GACAACACAG AGAATGACCT AAACCAGGGG GATGATCACG GCTTCAGTCC GTTGCACTGG 
GCGTGCCGCG AGGGCCGTTC GGGTGTTGTG GATATGCTCA TCATGAGAGG TGCTCGTATC 
AATGTGATGA ACCGCGGCGA CGACACACCC CTGCACCTGG CAGCGAGCCA TGGCCATCGT 
GACATTCTGG CCAAGCTGAT TCAGTGTAAA GCAGATACCA ATGCGGCAAA CGAGCACGGA 
AACACCCCTC TACATTATGC CTGCTTCTGG GCCCACGATC AGGTGGCGGA GGATCTGGTC 
AGTAATGGAG CTCAGGTGAG CATCTGCAAC AAGTATGGAG AAACTCCAAT GGACAAGGCC 
AAACCACCGC TTGCAGCAAA TCTAAAAGAC CTGGCAGAGA AACAAGGACA GAGCCTGTCA 
AAAGTCCCTT ACAAGGACAA TTTCTGGAAA GGAACAACTC GAACACGACC ACGTAATGGC 
ACACTAAACA AGCAAGCTGG CATAGATTAT AAGCAGCTTA ACATGCTGAA CAAGATCAAC 
GAGAACCACT CCGGCGAGCT GTGGAAGGGC CGCTGGCAGG GCACAGAGAT TGTGGTCAAG 
ATGCTGCATG TGCGGGAATG GACCACCAGA AAGAGCCGAG ACTTTAATGA GGAGTACCCT 
AAACTAAGAA TCTTCTCTCA CCCAAACGTG TTGCCCGTGT TGGGGGCGTG TCAGTCTCCA 
CCTGCCCCTC ACCCAATCAT CATCACACAC TCTATGCCCT ACGGTTCCCT CTACAACTTG 
CTGCATGAGG GCACCAACTT AGTAGTGGAT CAAAACCAGG CTGTGAAATT TGCTCTGGAC 
ATTGCCTGTG GAATGGCTTT CCTACACACT CTTGAGCCCA TGATCCCCCG CCATTATCTC 
AACAGCAAGA GTGTCATGAT TGACGAGGAC ATGACGGCCA GGATAAGCAT GGCAGATGTA 
AAGTTGTCAT TTCAGTGTCC TGGCAGGATG TACTCTCCTG CATGGGTGGC ACCTGAAGCT 
CTGCAGAAGA AGCCAGAGGA GATCAACCGG CGCTCAGCTG ACATGTGGAG CTACGCCGTG 
CTGCTTTGGG AGCTGGTGAG CAGAGAAGTG CCGTTTGCTG ACCTCTCAAA CATGGAGATC 
GGCATGAAGG TGGCGCTAGA GGGACTACGG CCCACCATCC CACCCGGCAT CTCCCCTCAC 
ATCTGCAAAC TCATGAAGAT CTGCATGAAC GAAGACCCTG CAAAGAGGCC CAAATTCGAC 
ATGATCGTGC CCATCCTGGA GAAGATGCAA GACAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

A mutation in the atrial-specific myosin light chain gene (MYL4) causes familial atrial fibrillation.
Nature communications711303(2016 Apr)
Orr N,Arnaout R,Gula LJ,Spears DA,Leong-Sit P,Li Q,Tarhuni W,Reischauer S,Chauhan VS,Borkovich M,Uppal S,Adler A,Coughlin SR,Stainier DY,Gollob MH

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

ilk cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報