irs2a cDNA ORF clone, Danio rerio(zebrafish)

The following irs2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the irs2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa33083 NM_200315.1
Latest version!
Danio rerio insulin receptor substrate 2a (irs2a), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
ODa147213 XM_021466764.1
Latest version!
Danio rerio insulin receptor substrate 2a (irs2a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa33083
    Clone ID Related Accession (Same CDS sequence) NM_200315.1
    Accession Version NM_200315.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3099bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-02
    Organism Danio rerio(zebrafish)
    Product insulin receptor substrate 2
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC050518.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC050518.1, DN896592.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCAAGTC CGCCTCTTAA AGGGGGACCC TCGCTATCAA ACAGCAACAA CATTCATTCA 
    AACAACATAC GAAAACATGG ATATCTCAAG AAGCAAAAAC ACGGACATCG GCGCTATTTT
    GTTTTGAAAG ACACGAGCGA CGGACTCCCA GCGCGCTTGG AATACCACGA GAATGAAAAG
    AAATGGAAAA ACAAGTCTGC TCCAAAAAGA GTGATATTCC TCGACTCGTG TTTGAGCATA
    AACAAGCGCG CTGACGCGAA ACACAGATAT TTGATAGCCC TCTATACCAA GGACGAATAT
    TTTGCTGTGG CTGCGGAGAA TGAGCAAGAG CAGGAGAGCT GGTACGGAGA CTTGACTGAT
    TTATTAAGCA AAGGAAAAGT GTGTGACAGC ACCGTTTCTA ATTCGGCATC CTCTCTGGTA
    GGCTTCGACG AGGGAAATTA CGGAATGATT ACACCGGTGA ATGCTGCTTA TAAAGAGGTA
    TGGCAAGTGA ACCTTAAAGC AAAGGGACTT GGGCAGAGCA GAAATATCAC TGGAGTTTTT
    AGGCTGTGTT TAACAAGTCG GACTATTAGT TTTGTGAAAC TTAACTCCGA GGTGGCATCA
    GTTACCCTAC AGTTGATGAA TATTCGTAGG TGTGGGCACT CTGATAGCTT TTTCTTTATT
    GAAGTTGGAA GATCAGCCTC TACTGGACCT GGAGAACTGT GGATGCAAGC AGACGATTCT
    GTGGTGGCAC AAAATATACA TGAGACAATT TTAGAAGCTA TGAAAGCAAT GAAAGAGCTG
    TCAGAGTTCA GACCCCGCAG CAAAAGCCAG TCATCAGGAT CAAACCCCAT TTCTGTACCC
    ACTCGTCGGA ACCTAAACAA TTTACCTCCA AGTCAAACAG GGCTAGTGAG GAGGTCAAGG
    ACGGACAGTA CAGCAGCCAC ATCTCCCGTG ACCAAATTTT CTTCTTGTCG AATACGGACT
    GCAAGTGAAG GGGAAGGTAC CATGATCAGG CCAGTCTCTA TATCAGTATC TGAGAATGGA
    AGCCCAATGA CTGTTGGCCG GAACCACATG AGTAGATCTA ATACAGTTTC AGTAGGCTGT
    CGGACATCAG AACCATCACT ACTTAATCAC AGCAGGTCCA TGTCTATGAC CATGTACCAC
    TCTCCCCCAT CTGTTGTAAG TCCACTAAGT TTGTCTTCAA TCAGTATGAA TGGTTCTATC
    TCAAACAAAG CACACAGACC TTCCAGCTGT AGTGGTTCTG TTTCTGGGTC ACCAAATGAT
    TTTGGCTTTT TAACGTGTGA GGATTACAGT TCCAGCCCAG GGGATGTGAG GTACAACCTA
    GCAAACAGGA GTGACACTCC TTCTTCTCTG TCCAGCACAC CACCCTTACG AGAAACCAGT
    GAACTCCATA GTTATATGGT GATGGACAGG CCATCCAACT TTTCACAGAA CAAGACTAGC
    AGAGACATGT TGGATGTGGA GACATACAGG AAGAGGACAT ATTCTCTAAC AACCCCACGG
    CAGCAGATGG CACACTCCCA GTTATCTTCA GCGTCACTTG ATGAATACAT GTTGATGAGG
    GCTGCGAACA GCCACTCTGG ACGGAGCACA AATTCTGCTT CTCCTAAACT TTCATATCCA
    GAAGATTATG GGGACATTGA GATAGGCTCT AACTGTAATG TGGGCGATGA TGGCTACATG
    CCAATGACAC CAGGCATGGC ACCTCAGAGT GCAAAAAATG ACCACTACAT GCCAATGAGT
    CCCATGAGTG TTTCAGCACC CAAGCAGATC ATTAACCCGA GATCTCATCC ACAGTCTCCA
    GGCAGTGGCT GCACAGCTAA CTCACCCAGC AGTGGATCTC TAGAGGACAG CGGGTACATG
    AAAATGTGGT CCGGATCCAA GTTTTCACTG GAGAGCTCAG ATGGAAAAGC TGCGAATGGA
    GAATATGTGA ATATGTCACC TGTTGACACA TCCATCTCAC CCACACCACC AGAATATTTT
    ATGGGTCCGT GTGAGCCAAC ACAACGGCCC TCCCCAACTC AAAGTATTCG CTTCAATAAG
    CAACAGGTGC CTAAAAGAGT AGATGATAAC CAGTATGTTT TCATGAGTCC TCAGAACCAA
    AATCAAGCCG AAGAGGCCAA CAGCGTTTCT TCTCTGCCCC CTCTCTATCG CCCTGATGTA
    ATGGCACAGA GATCAAAGGT CAACAGGCCC AATAGGTTAT CTCTAGACGC CCTAAGGATG
    CTTCCAAGGA TGACTGAACA TCCGCTACCA TGTGAGCCTA AAAGCCCTGG AGAGTACATA
    AACATAGACT TTGCTGCTGC TACACAGTAC TCCTCTCAAT CTATGGTCAC TGTGGAAAAT
    CAGGCCTCAC TGTCTAGCCT CAGACAGGGA TCTCCGCTCT CAGACTACAT GAACCTCAAC
    CAGAGTTCAC ACTCTTCTAA ACTGTGTGAA ATGAGTAGTC CCCTCGATGG ACTGCCAGAG
    CTGGCTGAAT GCCCGCCTGA TGATGGGACT TGTTTTCTCA ACGAACAGAG AAACTTACCA
    TGCCCCTCTG TATCAGGAAA AGGTAATTAC ACTGAGATGA GCTTCAACAG TGGTTCCCTT
    GAAAATCAAA AGAGTGCTTC AGTCAGCCCC ACTAACAGGG TTCAAAGACT TAACTTGGGC
    AACCAGGGGG TTTCAAAAGG AATTGGCGCT TTCTTGTTAG CTGCCTCCTC TGTAGATCCA
    AACGGAGGAG CAAAGGTGAT TCGAGCAGAC CCCCAGGGCC GGAGACGGCA TAGCTCTGAG
    ACCTTTTCAT CCACCACCAC AGTTGCACCG GTCTTTCCCT CTTTTGCACA CAATGTTAAG
    CGACACAGCT CTGCCTCCGT AGAAAACATC TCAGTACGAA GCAGTGAAGG TTCTGATGAG
    GAGTATGAGT CTCCTATGTG CCAAGAAATG TCAGCTGGTT ATCAAAATGG GCTCAATTAC
    ATCGCCTTAA ATCTCATGGA GAAACAAGAG ACTGGCAATT GTGAAAATCT TGTGGGCTTT
    AAGACTGGCG GTTGTTGTAA AGCAGGAATA AATGGATTAC ACACAACTCC CTATGTGTGT
    CTTGGATTCA AAGAAACTGC AACCACTGTA CAAGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_956609.1
    CDS152..3250
    Translation

    Target ORF information:

    RefSeq Version NM_200315.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio insulin receptor substrate 2a (irs2a), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_200315.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCAAGTC CGCCTCTTAA AGGGGGACCC TCGCTATCAA ACAGCAACAA CATTCATTCA 
    AACAACATAC GAAAACATGG ATATCTCAAG AAGCAAAAAC ACGGACATCG GCGCTATTTT
    GTTTTGAAAG ACACGAGCGA CGGACTCCCA GCGCGCTTGG AATACCACGA GAATGAAAAG
    AAATGGAAAA ACAAGTCTGC TCCAAAAAGA GTGATATTCC TCGACTCGTG TTTGAGCATA
    AACAAGCGCG CTGACGCGAA ACACAGATAT TTGATAGCCC TCTATACCAA GGACGAATAT
    TTTGCTGTGG CTGCGGAGAA TGAGCAAGAG CAGGAGAGCT GGTACGGAGA CTTGACTGAT
    TTATTAAGCA AAGGAAAAGT GTGTGACAGC ACCGTTTCTA ATTCGGCATC CTCTCTGGTA
    GGCTTCGACG AGGGAAATTA CGGAATGATT ACACCGGTGA ATGCTGCTTA TAAAGAGGTA
    TGGCAAGTGA ACCTTAAAGC AAAGGGACTT GGGCAGAGCA GAAATATCAC TGGAGTTTTT
    AGGCTGTGTT TAACAAGTCG GACTATTAGT TTTGTGAAAC TTAACTCCGA GGTGGCATCA
    GTTACCCTAC AGTTGATGAA TATTCGTAGG TGTGGGCACT CTGATAGCTT TTTCTTTATT
    GAAGTTGGAA GATCAGCCTC TACTGGACCT GGAGAACTGT GGATGCAAGC AGACGATTCT
    GTGGTGGCAC AAAATATACA TGAGACAATT TTAGAAGCTA TGAAAGCAAT GAAAGAGCTG
    TCAGAGTTCA GACCCCGCAG CAAAAGCCAG TCATCAGGAT CAAACCCCAT TTCTGTACCC
    ACTCGTCGGA ACCTAAACAA TTTACCTCCA AGTCAAACAG GGCTAGTGAG GAGGTCAAGG
    ACGGACAGTA CAGCAGCCAC ATCTCCCGTG ACCAAATTTT CTTCTTGTCG AATACGGACT
    GCAAGTGAAG GGGAAGGTAC CATGATCAGG CCAGTCTCTA TATCAGTATC TGAGAATGGA
    AGCCCAATGA CTGTTGGCCG GAACCACATG AGTAGATCTA ATACAGTTTC AGTAGGCTGT
    CGGACATCAG AACCATCACT ACTTAATCAC AGCAGGTCCA TGTCTATGAC CATGTACCAC
    TCTCCCCCAT CTGTTGTAAG TCCACTAAGT TTGTCTTCAA TCAGTATGAA TGGTTCTATC
    TCAAACAAAG CACACAGACC TTCCAGCTGT AGTGGTTCTG TTTCTGGGTC ACCAAATGAT
    TTTGGCTTTT TAACGTGTGA GGATTACAGT TCCAGCCCAG GGGATGTGAG GTACAACCTA
    GCAAACAGGA GTGACACTCC TTCTTCTCTG TCCAGCACAC CACCCTTACG AGAAACCAGT
    GAACTCCATA GTTATATGGT GATGGACAGG CCATCCAACT TTTCACAGAA CAAGACTAGC
    AGAGACATGT TGGATGTGGA GACATACAGG AAGAGGACAT ATTCTCTAAC AACCCCACGG
    CAGCAGATGG CACACTCCCA GTTATCTTCA GCGTCACTTG ATGAATACAT GTTGATGAGG
    GCTGCGAACA GCCACTCTGG ACGGAGCACA AATTCTGCTT CTCCTAAACT TTCATATCCA
    GAAGATTATG GGGACATTGA GATAGGCTCT AACTGTAATG TGGGCGATGA TGGCTACATG
    CCAATGACAC CAGGCATGGC ACCTCAGAGT GCAAAAAATG ACCACTACAT GCCAATGAGT
    CCCATGAGTG TTTCAGCACC CAAGCAGATC ATTAACCCGA GATCTCATCC ACAGTCTCCA
    GGCAGTGGCT GCACAGCTAA CTCACCCAGC AGTGGATCTC TAGAGGACAG CGGGTACATG
    AAAATGTGGT CCGGATCCAA GTTTTCACTG GAGAGCTCAG ATGGAAAAGC TGCGAATGGA
    GAATATGTGA ATATGTCACC TGTTGACACA TCCATCTCAC CCACACCACC AGAATATTTT
    ATGGGTCCGT GTGAGCCAAC ACAACGGCCC TCCCCAACTC AAAGTATTCG CTTCAATAAG
    CAACAGGTGC CTAAAAGAGT AGATGATAAC CAGTATGTTT TCATGAGTCC TCAGAACCAA
    AATCAAGCCG AAGAGGCCAA CAGCGTTTCT TCTCTGCCCC CTCTCTATCG CCCTGATGTA
    ATGGCACAGA GATCAAAGGT CAACAGGCCC AATAGGTTAT CTCTAGACGC CCTAAGGATG
    CTTCCAAGGA TGACTGAACA TCCGCTACCA TGTGAGCCTA AAAGCCCTGG AGAGTACATA
    AACATAGACT TTGCTGCTGC TACACAGTAC TCCTCTCAAT CTATGGTCAC TGTGGAAAAT
    CAGGCCTCAC TGTCTAGCCT CAGACAGGGA TCTCCGCTCT CAGACTACAT GAACCTCAAC
    CAGAGTTCAC ACTCTTCTAA ACTGTGTGAA ATGAGTAGTC CCCTCGATGG ACTGCCAGAG
    CTGGCTGAAT GCCCGCCTGA TGATGGGACT TGTTTTCTCA ACGAACAGAG AAACTTACCA
    TGCCCCTCTG TATCAGGAAA AGGTAATTAC ACTGAGATGA GCTTCAACAG TGGTTCCCTT
    GAAAATCAAA AGAGTGCTTC AGTCAGCCCC ACTAACAGGG TTCAAAGACT TAACTTGGGC
    AACCAGGGGG TTTCAAAAGG AATTGGCGCT TTCTTGTTAG CTGCCTCCTC TGTAGATCCA
    AACGGAGGAG CAAAGGTGAT TCGAGCAGAC CCCCAGGGCC GGAGACGGCA TAGCTCTGAG
    ACCTTTTCAT CCACCACCAC AGTTGCACCG GTCTTTCCCT CTTTTGCACA CAATGTTAAG
    CGACACAGCT CTGCCTCCGT AGAAAACATC TCAGTACGAA GCAGTGAAGG TTCTGATGAG
    GAGTATGAGT CTCCTATGTG CCAAGAAATG TCAGCTGGTT ATCAAAATGG GCTCAATTAC
    ATCGCCTTAA ATCTCATGGA GAAACAAGAG ACTGGCAATT GTGAAAATCT TGTGGGCTTT
    AAGACTGGCG GTTGTTGTAA AGCAGGAATA AATGGATTAC ACACAACTCC CTATGTGTGT
    CTTGGATTCA AAGAAACTGC AACCACTGTA CAAGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa147213
    Clone ID Related Accession (Same CDS sequence) XM_021466764.1
    Accession Version XM_021466764.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product insulin receptor substrate 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCAAGTC CGCCTCTTAA AGGGGGACCC TCGCTATCAA ACAGCAACAA CATTCATTCA 
    AACAACATAC GAAAACATGG ATATCTCAAG AAGCAAAAAC ACGGACATCG GCGCTATTTT
    GTTTTGAAAG ACACGAGCGA CGGACTCCCA GCGCGCTTGG AATACCACGA GAATGAAAAG
    AAATGGAAAA ACAAGTCTGC TCCAAAAAGA GTGATATTCC TCGACTCGTG TTTGAGCATA
    AACAAGCGCG CTGACGCGAA ACACAGATAT TTGATAGCCC TCTATACCAA GGACGAATAT
    TTTGCTGTGG CTGCGGAGAA TGAGCAAGAG CAGGAGAGCT GGTACGGAGA CTTGGCTGAT
    TTATTAAGCA AAGGAAAAGT GTGTGACAGC ACCGTTTCTA ATTCGGCATC CTCTCTGGTA
    GGCTTCGACG AGGGAAATTA CGGAATGATT ACACCGGTGA ATGCTGCTTA TAAAGAGGTA
    TGGCAAGTGA ACCTTAAAGC AAAGGGACTT GGGCAGAGCA GAAATATCAC TGGAGTTTTT
    AGGCTGTGTT TAACAAGTCG GACTATTAGT TTTGTGAAAC TTAACTCCGA GGTGGCATCA
    GTTACCCTAC AGTTGATGAA TATTCGTAGG TGTGGGCACT CTGATAGCTT TTTCTTTATT
    GAAGTTGGAA GATCAGCCTC TACTGGACCT GGAGAACTGT GGATGCAAGC AGACGATTCT
    GTGGTGGCAC AAAATATACA TGAGACAATT TTAGAAGCTA TGAAAGCAAT GAAAGAGCTG
    TCAGAGTTCA GACCCCGCAG CAAAAGTCAG TCATCAGGAT CAAACCCCAT TTCTGTACCC
    ACTCGTCGGA ACCTAAACAA TTTACCTCCA AGTCAAACAG GGCTAGTGAG GAGGTCAAGG
    ACGGACAGTA CAGCAGCCAC ATCTCCCGTG ACCAAATTTT CTTCTTGTCG AATACGGACT
    GCAAGTGAAG GGGAAGGTAC CATGATCAGG CCAGTCTCTG TATCAGTATC TGAGAATGGA
    AGCCCAATGA CTGTTGGCCG GCACCACATG AGTAGATCTA ATACTGTTTC AGTAGGCTGT
    CGGACATCAG AACCATCACT ACTTAATCAC AGCAGGTCCA TGTCTATGAC CATGTACCAC
    TCTCCCCCAT CTGTTGTAAG TCCACTAAGT TTGTCTTCAA TCAGTATGAA TGGTTCTATC
    TCAAACAAAG CACACAGACC TTCCAGCTGT AGTGGTTCTG TTTCTGGGTC ACCAAATGAT
    TTTGGCTTTT TAACGTGTGA GGATTACAGT TCCAGCCCAG GGGATGTGAG GTACAACCTA
    GCAAACAGGA GTGACACTCC TTCTTCTCTG TCCAGCACAC CACCCTTACG AGAAACCAGT
    GAACTCCATA GTTATATGGT GATGGACAGG CCATCCAACT TTTCACAGAA CAAGACTAGC
    AGAGACATGT TGGATGTGGA GACATACAGG AAGAGGACAT ATTCTCTAAC AACCCCACGG
    CAACAGATGG CACACTCCCA GTTATCTTCA GCGTCACTTG ATGAATACAT GTTGATGAGG
    GCTGCGAACA GCCACTCTGG ACGGAGCACA AATTCTGCTT CTCCTAAACT TTCATATCCA
    GAAGATTATG GGGACATTGA GATAGGCTCT AACTGTAATG TGGGTGATGA TGGCTACATG
    CCAATGACAC CAGGCATGGC ACCTCAGAGT GCAAAAAATG ACCACTACAT GCCAATGAGT
    CCCATGAGTG TTTCAGCACC CAAGCAGATC ATTAACCCGA GATCTCATCC ACAGTCTCCA
    GGCAGTGGCT GCACAGCTAA CTCCCCCAGC AGTGGATCTC TAGAGGACAG CGGGTACATG
    AAAATGTGGT CCGGATCCAA GTTTTCACTG GAGAGCTCAG ATGGAAAAGC AGCGAATGGA
    GAATATGTGA ATATGTCACC TGTTGACACA TCCATCTCAC CCACACCACC AGAATATTTT
    ATGGGTCCTT GTGAGCCAAC ACAACGGCCC TCCCCAACTC AAAGTATTCG CTTCAATAAG
    CAACAGGTGC CTAAAAGAGT AGATGATAAC CAGTATGTTT TCATGAGTCC TCAGAACCAA
    AATCAAACCG AAGAGGCCAA CAGCGTTTCT TCTCTGCCCC CTCTCTATCG CCCTGATGTA
    ATGGCACAGA GATCAAAGGT CAACAGGCCC AATAGGTTAT CTCTAGACGC TCTAAGGATG
    CTTCCAAGGA TGACTGAACA TCCGCTACCA TGTGAGCCTA AAAGCCCTGG AGAGTACATA
    AACATAGACT TTGCTGCTGC TACTCAGTAC TCCTCTCAAT CTATGGTCAC TGTGGAAAAT
    CAGGCCTCAC TGTCCAGCCT CAGACAGGGA TCTCCGCTCT CAGACTACAT GAACCTCAAC
    CAGAGTTCAC ACTCTTCTAA ACTGTGTGAA ATGAGTAGTC CCCTCGATGG ACTGCCAGAG
    CTGGCTGAAT GCCCGCCTGA TGATGGGACT TGTTTTCTCA ACGAACAGAG AAACTTACCA
    TGCCCCTCTG TATCAGGAAA AGGTAATTAC ACTGAGATGA GCTTCAACAG TGGTTCCCTT
    GAAAATCAAA AGAGTGCTTC AGTCAGCCCC ACTAGCAGGG TTCAAAGGCT TAACTTGGGC
    AACCAGGGGG TTTCAAAAGG AATTGGCGCT TTCTTGTTAG CTGCCTCCTC TGTAGATCCA
    AACGGAGGAG CAAAGGTGAT TCGAGCAGAC CCCCAGGGCC GGAGACGGCA TAGCTCTGAG
    ACCTTTTCAT CCACCACCAC AGTTGCACCG GTCTTTCCCT CTTTTGCACA CAATGTTAAG
    CGACACAGCT CTGCCTCCGT AGAAAACATC TCAGTACGAA GCAGTGAAGG TTCTGATGAG
    GAGTATGAGT CTCCTATGTG CCAAGAAATG TCAGCTGGTT ATCAAAATGG GCTCAATTAC
    ATCGCCTTAA ATCTCATGGA GAAACAAGAG ACTGGCAATT GTGAAAATCT TGTGGGCTTT
    AAGACTGGCG GTTGTTGTAA AGCAGGAATA AATGGATTAC ACACAACTCC CTATGTGTGT
    CTTGGATTCA AAGAAACTGC AACCACTGTA CAAGTACTCC TTAAGACGAT TGTGTCCATT
    TGCATGTGTG GCAGATGGCT GAATAGATGT GGGGCTGTGT GTGTGTGTGC TCGCGTGCAC
    CTGCCGCTCT GCTCACCGCA GATTGAGCAG GAAGCAGAGG TTACTCCTAA TGCTAAGGGA
    AAATGGAAGC TCAACCATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322439.1
    CDS302..3562
    Translation

    Target ORF information:

    RefSeq Version XM_021466764.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio insulin receptor substrate 2a (irs2a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021466764.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCAAGTC CGCCTCTTAA AGGGGGACCC TCGCTATCAA ACAGCAACAA CATTCATTCA 
    AACAACATAC GAAAACATGG ATATCTCAAG AAGCAAAAAC ACGGACATCG GCGCTATTTT
    GTTTTGAAAG ACACGAGCGA CGGACTCCCA GCGCGCTTGG AATACCACGA GAATGAAAAG
    AAATGGAAAA ACAAGTCTGC TCCAAAAAGA GTGATATTCC TCGACTCGTG TTTGAGCATA
    AACAAGCGCG CTGACGCGAA ACACAGATAT TTGATAGCCC TCTATACCAA GGACGAATAT
    TTTGCTGTGG CTGCGGAGAA TGAGCAAGAG CAGGAGAGCT GGTACGGAGA CTTGGCTGAT
    TTATTAAGCA AAGGAAAAGT GTGTGACAGC ACCGTTTCTA ATTCGGCATC CTCTCTGGTA
    GGCTTCGACG AGGGAAATTA CGGAATGATT ACACCGGTGA ATGCTGCTTA TAAAGAGGTA
    TGGCAAGTGA ACCTTAAAGC AAAGGGACTT GGGCAGAGCA GAAATATCAC TGGAGTTTTT
    AGGCTGTGTT TAACAAGTCG GACTATTAGT TTTGTGAAAC TTAACTCCGA GGTGGCATCA
    GTTACCCTAC AGTTGATGAA TATTCGTAGG TGTGGGCACT CTGATAGCTT TTTCTTTATT
    GAAGTTGGAA GATCAGCCTC TACTGGACCT GGAGAACTGT GGATGCAAGC AGACGATTCT
    GTGGTGGCAC AAAATATACA TGAGACAATT TTAGAAGCTA TGAAAGCAAT GAAAGAGCTG
    TCAGAGTTCA GACCCCGCAG CAAAAGTCAG TCATCAGGAT CAAACCCCAT TTCTGTACCC
    ACTCGTCGGA ACCTAAACAA TTTACCTCCA AGTCAAACAG GGCTAGTGAG GAGGTCAAGG
    ACGGACAGTA CAGCAGCCAC ATCTCCCGTG ACCAAATTTT CTTCTTGTCG AATACGGACT
    GCAAGTGAAG GGGAAGGTAC CATGATCAGG CCAGTCTCTG TATCAGTATC TGAGAATGGA
    AGCCCAATGA CTGTTGGCCG GCACCACATG AGTAGATCTA ATACTGTTTC AGTAGGCTGT
    CGGACATCAG AACCATCACT ACTTAATCAC AGCAGGTCCA TGTCTATGAC CATGTACCAC
    TCTCCCCCAT CTGTTGTAAG TCCACTAAGT TTGTCTTCAA TCAGTATGAA TGGTTCTATC
    TCAAACAAAG CACACAGACC TTCCAGCTGT AGTGGTTCTG TTTCTGGGTC ACCAAATGAT
    TTTGGCTTTT TAACGTGTGA GGATTACAGT TCCAGCCCAG GGGATGTGAG GTACAACCTA
    GCAAACAGGA GTGACACTCC TTCTTCTCTG TCCAGCACAC CACCCTTACG AGAAACCAGT
    GAACTCCATA GTTATATGGT GATGGACAGG CCATCCAACT TTTCACAGAA CAAGACTAGC
    AGAGACATGT TGGATGTGGA GACATACAGG AAGAGGACAT ATTCTCTAAC AACCCCACGG
    CAACAGATGG CACACTCCCA GTTATCTTCA GCGTCACTTG ATGAATACAT GTTGATGAGG
    GCTGCGAACA GCCACTCTGG ACGGAGCACA AATTCTGCTT CTCCTAAACT TTCATATCCA
    GAAGATTATG GGGACATTGA GATAGGCTCT AACTGTAATG TGGGTGATGA TGGCTACATG
    CCAATGACAC CAGGCATGGC ACCTCAGAGT GCAAAAAATG ACCACTACAT GCCAATGAGT
    CCCATGAGTG TTTCAGCACC CAAGCAGATC ATTAACCCGA GATCTCATCC ACAGTCTCCA
    GGCAGTGGCT GCACAGCTAA CTCCCCCAGC AGTGGATCTC TAGAGGACAG CGGGTACATG
    AAAATGTGGT CCGGATCCAA GTTTTCACTG GAGAGCTCAG ATGGAAAAGC AGCGAATGGA
    GAATATGTGA ATATGTCACC TGTTGACACA TCCATCTCAC CCACACCACC AGAATATTTT
    ATGGGTCCTT GTGAGCCAAC ACAACGGCCC TCCCCAACTC AAAGTATTCG CTTCAATAAG
    CAACAGGTGC CTAAAAGAGT AGATGATAAC CAGTATGTTT TCATGAGTCC TCAGAACCAA
    AATCAAACCG AAGAGGCCAA CAGCGTTTCT TCTCTGCCCC CTCTCTATCG CCCTGATGTA
    ATGGCACAGA GATCAAAGGT CAACAGGCCC AATAGGTTAT CTCTAGACGC TCTAAGGATG
    CTTCCAAGGA TGACTGAACA TCCGCTACCA TGTGAGCCTA AAAGCCCTGG AGAGTACATA
    AACATAGACT TTGCTGCTGC TACTCAGTAC TCCTCTCAAT CTATGGTCAC TGTGGAAAAT
    CAGGCCTCAC TGTCCAGCCT CAGACAGGGA TCTCCGCTCT CAGACTACAT GAACCTCAAC
    CAGAGTTCAC ACTCTTCTAA ACTGTGTGAA ATGAGTAGTC CCCTCGATGG ACTGCCAGAG
    CTGGCTGAAT GCCCGCCTGA TGATGGGACT TGTTTTCTCA ACGAACAGAG AAACTTACCA
    TGCCCCTCTG TATCAGGAAA AGGTAATTAC ACTGAGATGA GCTTCAACAG TGGTTCCCTT
    GAAAATCAAA AGAGTGCTTC AGTCAGCCCC ACTAGCAGGG TTCAAAGGCT TAACTTGGGC
    AACCAGGGGG TTTCAAAAGG AATTGGCGCT TTCTTGTTAG CTGCCTCCTC TGTAGATCCA
    AACGGAGGAG CAAAGGTGAT TCGAGCAGAC CCCCAGGGCC GGAGACGGCA TAGCTCTGAG
    ACCTTTTCAT CCACCACCAC AGTTGCACCG GTCTTTCCCT CTTTTGCACA CAATGTTAAG
    CGACACAGCT CTGCCTCCGT AGAAAACATC TCAGTACGAA GCAGTGAAGG TTCTGATGAG
    GAGTATGAGT CTCCTATGTG CCAAGAAATG TCAGCTGGTT ATCAAAATGG GCTCAATTAC
    ATCGCCTTAA ATCTCATGGA GAAACAAGAG ACTGGCAATT GTGAAAATCT TGTGGGCTTT
    AAGACTGGCG GTTGTTGTAA AGCAGGAATA AATGGATTAC ACACAACTCC CTATGTGTGT
    CTTGGATTCA AAGAAACTGC AACCACTGTA CAAGTACTCC TTAAGACGAT TGTGTCCATT
    TGCATGTGTG GCAGATGGCT GAATAGATGT GGGGCTGTGT GTGTGTGTGC TCGCGTGCAC
    CTGCCGCTCT GCTCACCGCA GATTGAGCAG GAAGCAGAGG TTACTCCTAA TGCTAAGGGA
    AAATGGAAGC TCAACCATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.