fen1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	fen1
Entrez Gene ID	386707
Full Name	flap structure-specific endonuclease 1
Synonyms	FEN-1,cb879
General protein information	Preferred Names flap structure-specific endonuclease 1 Names flap endonuclease 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017353804.1	XP_017209293.1	flap endonuclease 1 isoform X1
NM_001328507.1	NP_001315436.1	flap endonuclease 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	FEN1	NP_004102.1
Canis lupus familiaris (dog)	FEN1	XP_005631709.1
Gallus gallus (chicken)	FEN1	NP_001072959.1
Drosophila melanogaster (fruit fly)	Fen1	NP_523765.1
Xenopus tropicalis (tropical clawed frog)	fen1	NP_001017005.1
	FEN1	XP_002799575.1
Bos taurus (cattle)	FEN1	NP_001030285.1
Rattus norvegicus (Norway rat)	Fen1	NP_445882.1
Danio rerio (zebrafish)	fen1	NP_942115.1
Caenorhabditis elegans (roundworm)	crn-1	NP_491168.1
Kluyveromyces lactis	KLLA0F02992g	XP_455215.1
Neurospora crassa	NCU10776	XP_001728355.1
Arabidopsis thaliana (thale cress)	AT5G26680	NP_001154740.1
Mus musculus (house mouse)	Fen1	NP_032025.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011448	XP_317855.2
Saccharomyces cerevisiae (baker's yeast)	RAD27	NP_012809.1
Oryza sativa (rice)	Os05g0540100	NP_001056179.1
Pan troglodytes (chimpanzee)	FEN1	XP_003951955.1
Eremothecium gossypii	AGOS_ABL052C	NP_982895.2
Schizosaccharomyces pombe (fission yeast)	rad2	NP_594972.1
Magnaporthe oryzae (rice blast fungus)	MGG_03419	XP_003716519.1

Gene Ontology
Bibliography

Related articles in PubMed

Oxidative stress, cell cycle arrest, DNA damage and apoptosis in adult zebrafish (Danio rerio) induced by tris(1,3-dichloro-2-propyl) phosphate.
Chen H, Wang P, Du Z, Wang G, Gao S
Aquatic toxicology (Amsterdam, Netherlands)19437-45(2018 Jan)

Effects of starch-coating of magnetite nanoparticles on cellular uptake, toxicity and gene expression profiles in adult zebrafish.
Zheng M, Lu J, Zhao D
The Science of the total environment622-623930-941(2017 Dec)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

TPhP exposure disturbs carbohydrate metabolism, lipid metabolism, and the DNA damage repair system in zebrafish liver.
Du Z, Zhang Y, Wang G, Peng J, Wang Z, Gao S
Scientific reports621827(2016 Feb)

Epigenetic Studies Point to DNA Replication/Repair Genes as a Basis for the Heritable Nature of Long Term Complications in Diabetes.
Leontovich AA, Intine RV, Sarras MP Jr
Journal of diabetes research20162860780(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following fen1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the fen1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa57574	NM_001328507.1 Latest version!	Danio rerio flap structure-specific endonuclease 1 (fen1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
ODa57574	XM_017353804.1 Latest version!	Danio rerio flap structure-specific endonuclease 1 (fen1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

fen1 ( NM_001328507.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001315436.1 Danio rerio flap structure-specific endonuclease 1 (fen1), mRNA.

Price & Availability↑

CloneID	ODa57574
Clone ID Related Accession (Same CDS sequence)	XM_017353804.1 , NM_001328507.1
Accession Version	NM_001328507.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-20
Organism	Danio rerio(zebrafish)
Product	flap endonuclease 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CABZ01101620.1, EH439441.1, CT612850.2 and CT678576.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC071488.1, AY648844.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGGAATTC ACGGACTTGC AAAGCTCATT GCAGATCACG CGCCTTCGGC TATTAAGGAG 
CATGAAATCA AAAGCTACTT TGGACGGAAG ATTGCTATAG ATGCCTCCAT GTGCATCTAC 
CAGTTTTTGA TTGCGGTTCG ACAGGACGGT AATGTGTTGC AGAATGAAGA TGGAGAGACC 
ACTAGCCACC TCATGGGCAT GTTCTACAGG ACCATCCGAA TGCTGGAAAG CGGCATCAAG 
CCGGTTTATG TTTTTGATGG GAAGCCACCT CAGCTCAAGT CAGGAGAGCT TGAGAAGAGA 
GTTGAGAGAC GAGCAGAGGC TGAGAAACTT CTAGCTCAGG CACAAGAAGC AGGTGAGCAG 
GAAAATATTG ACAAGTTTAG TAAACGACTA GTGAAGGTAA CCAAACAACA CAATGAGGAA 
TGCAAGAAAT TACTTTCTCT AATGGGAGTC CCATACATTG AGGCTCCATG TGAGGCAGAG 
GCGAGTTGTG CTGCTCTGGT GAAAGCAGGG AAGGTTTATG CCACAGCAAC AGAAGATATG 
GACGGTCTGA CATTTGGAAC AACGGTCTTA TTGAGGCATC TAACAGCTAG TGAAGCAAAA 
AAGCTCCCCA TCCAAGAGTT TCACTTCAGT CGTATTCTGC AGGATATGGA GCTGACGCAT 
CAACAGTTCA TCGACCTGTG TATCCTGCTG GGCTGTGACT ACTGTGGCAC TATAAAGGGA 
ATCGGACCCA AGAGGGCCAT TGACCTAATT AAACAGCATG GCTCTATTGA AGAAATTCTG 
GAGAATATTG ACCCAAATAA GCACCCTGCT CCAGAGGACT GGCTGTATAA AGAGGCTCGG 
GGTCTCTTCT TGGAACCTGA GGTCGTGGAT GGCACCTCTG TCGATCTGAA GTGGAATGAA 
CCTGATGAGG ATGGGCTAAT TCAGTTCATG TGTGCTGAGA AGCAGTTCAG TGAGGATCGT 
ATCCGTAACG GCTGTAAGAA GATCACAAAG AGCAGACAAG GCAGCACCCA GGGCCGACTG 
GACACATTCT TTACTGTAAC CGGATCAATC TCTTCTAAGC GTAAGGAGCC TGAAACAAAG 
GGATCTGCCA AAAAGAAACA AAAGACAAGT GCTACGCCAG GCAAATTTAA GAAGGGCAAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001315436.1
CDS	132..1274
Translation	MGIHGLAKLIADHAPSAIKEHEIKSYFGRKIAIDASMCIYQFLIAVRQDGNVLQNEDGETTSHLMGMFYRTIRMLESGIKPVYVFDGKPPQLKSGELEKRVERRAEAEKLLAQAQEAGEQENIDKFSKRLVKVTKQHNEECKKLLSLMGVPYIEAPCEAEASCAALVKAGKVYATATEDMDGLTFGTTVLLRHLTASEAKKLPIQEFHFSRILQDMELTHQQFIDLCILLGCDYCGTIKGIGPKRAIDLIKQHGSIEEILENIDPNKHPAPEDWLYKEARGLFLEPEVVDGTSVDLKWNEPDEDGLIQFMCAEKQFSEDRIRNGCKKITKSRQGSTQGRLDTFFTVTGSISSKRKEPETKGSAKKKQKTSATPGKFKKGK

Target ORF information:

RefSeq Version	NM_001328507.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio flap structure-specific endonuclease 1 (fen1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001328507.1

ORF Insert Sequence:

ATGGGAATTC ACGGACTTGC AAAGCTCATT GCAGATCACG CGCCTTCGGC TATTAAGGAG 
CATGAAATCA AAAGCTACTT TGGACGGAAG ATTGCTATAG ATGCCTCCAT GTGCATCTAC 
CAGTTTTTGA TTGCGGTTCG ACAGGACGGT AATGTGTTGC AGAATGAAGA TGGAGAGACC 
ACTAGCCACC TCATGGGCAT GTTCTACAGG ACCATCCGAA TGCTGGAAAG CGGCATCAAG 
CCGGTTTATG TTTTTGATGG GAAGCCACCT CAGCTCAAGT CAGGAGAGCT TGAGAAGAGA 
GTTGAGAGAC GAGCAGAGGC TGAGAAACTT CTAGCTCAGG CACAAGAAGC AGGTGAGCAG 
GAAAATATTG ACAAGTTTAG TAAACGACTA GTGAAGGTAA CCAAACAACA CAATGAGGAA 
TGCAAGAAAT TACTTTCTCT AATGGGAGTC CCATACATTG AGGCTCCATG TGAGGCAGAG 
GCGAGTTGTG CTGCTCTGGT GAAAGCAGGG AAGGTTTATG CCACAGCAAC AGAAGATATG 
GACGGTCTGA CATTTGGAAC AACGGTCTTA TTGAGGCATC TAACAGCTAG TGAAGCAAAA 
AAGCTCCCCA TCCAAGAGTT TCACTTCAGT CGTATTCTGC AGGATATGGA GCTGACGCAT 
CAACAGTTCA TCGACCTGTG TATCCTGCTG GGCTGTGACT ACTGTGGCAC TATAAAGGGA 
ATCGGACCCA AGAGGGCCAT TGACCTAATT AAACAGCATG GCTCTATTGA AGAAATTCTG 
GAGAATATTG ACCCAAATAA GCACCCTGCT CCAGAGGACT GGCTGTATAA AGAGGCTCGG 
GGTCTCTTCT TGGAACCTGA GGTCGTGGAT GGCACCTCTG TCGATCTGAA GTGGAATGAA 
CCTGATGAGG ATGGGCTAAT TCAGTTCATG TGTGCTGAGA AGCAGTTCAG TGAGGATCGT 
ATCCGTAACG GCTGTAAGAA GATCACAAAG AGCAGACAAG GCAGCACCCA GGGCCGACTG 
GACACATTCT TTACTGTAAC CGGATCAATC TCTTCTAAGC GTAAGGAGCC TGAAACAAAG 
GGATCTGCCA AAAAGAAACA AAAGACAAGT GCTACGCCAG GCAAATTTAA GAAGGGCAAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fen1 ( XM_017353804.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209293.1 Danio rerio flap structure-specific endonuclease 1 (fen1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa57574
Clone ID Related Accession (Same CDS sequence)	XM_017353804.1 , NM_001328507.1
Accession Version	XM_017353804.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	flap endonuclease 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAATTC ACGGACTTGC AAAGCTCATT GCAGATCACG CGCCTTCGGC TATTAAGGAG 
CATGAAATCA AAAGCTACTT TGGACGGAAG ATTGCTATAG ATGCCTCCAT GTGCATCTAC 
CAGTTTTTGA TTGCGGTTCG ACAGGACGGT AATGTGTTGC AGAATGAAGA TGGAGAGACC 
ACTAGCCACC TCATGGGCAT GTTCTACAGG ACCATCCGAA TGCTGGAAAG CGGCATCAAG 
CCGGTTTATG TTTTTGATGG GAAGCCACCT CAGCTCAAGT CAGGAGAGCT TGAGAAGAGA 
GTTGAGAGAC GAGCAGAGGC TGAGAAACTT CTAGCTCAGG CACAAGAAGC AGGTGAGCAG 
GAAAATATTG ACAAGTTTAG TAAACGACTA GTGAAGGTAA CCAAACAACA CAATGAGGAA 
TGCAAGAAAT TACTTTCTCT AATGGGAGTC CCATACATTG AGGCTCCATG TGAGGCAGAG 
GCGAGTTGTG CTGCTCTGGT GAAAGCAGGG AAGGTTTATG CCACAGCAAC AGAAGATATG 
GACGGTCTGA CATTTGGAAC AACGGTCTTA TTGAGGCATC TAACAGCTAG TGAAGCAAAA 
AAGCTCCCCA TCCAAGAGTT TCACTTCAGT CGTATTCTGC AGGATATGGA GCTGACGCAT 
CAACAGTTCA TCGACCTGTG TATCCTGCTG GGCTGTGACT ACTGTGGCAC TATAAAGGGA 
ATCGGACCCA AGAGGGCCAT TGACCTAATT AAACAGCATG GCTCTATTGA AGAAATTCTG 
GAGAATATTG ACCCAAATAA GCACCCTGCT CCAGAGGACT GGCTGTATAA AGAGGCTCGG 
GGTCTCTTCT TGGAACCTGA GGTCGTGGAT GGCACCTCTG TCGATCTGAA GTGGAATGAA 
CCTGATGAGG ATGGGCTAAT TCAGTTCATG TGTGCTGAGA AGCAGTTCAG TGAGGATCGT 
ATCCGTAACG GCTGTAAGAA GATCACAAAG AGCAGACAAG GCAGCACCCA GGGCCGACTG 
GACACATTCT TTACTGTAAC CGGATCAATC TCTTCTAAGC GTAAGGAGCC TGAAACAAAG 
GGATCTGCCA AAAAGAAACA AAAGACAAGT GCTACGCCAG GCAAATTTAA GAAGGGCAAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209293.1
CDS	150..1292
Translation	MGIHGLAKLIADHAPSAIKEHEIKSYFGRKIAIDASMCIYQFLIAVRQDGNVLQNEDGETTSHLMGMFYRTIRMLESGIKPVYVFDGKPPQLKSGELEKRVERRAEAEKLLAQAQEAGEQENIDKFSKRLVKVTKQHNEECKKLLSLMGVPYIEAPCEAEASCAALVKAGKVYATATEDMDGLTFGTTVLLRHLTASEAKKLPIQEFHFSRILQDMELTHQQFIDLCILLGCDYCGTIKGIGPKRAIDLIKQHGSIEEILENIDPNKHPAPEDWLYKEARGLFLEPEVVDGTSVDLKWNEPDEDGLIQFMCAEKQFSEDRIRNGCKKITKSRQGSTQGRLDTFFTVTGSISSKRKEPETKGSAKKKQKTSATPGKFKKGK

Target ORF information:

RefSeq Version	XM_017353804.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio flap structure-specific endonuclease 1 (fen1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353804.1

ORF Insert Sequence:

ATGGGAATTC ACGGACTTGC AAAGCTCATT GCAGATCACG CGCCTTCGGC TATTAAGGAG 
CATGAAATCA AAAGCTACTT TGGACGGAAG ATTGCTATAG ATGCCTCCAT GTGCATCTAC 
CAGTTTTTGA TTGCGGTTCG ACAGGACGGT AATGTGTTGC AGAATGAAGA TGGAGAGACC 
ACTAGCCACC TCATGGGCAT GTTCTACAGG ACCATCCGAA TGCTGGAAAG CGGCATCAAG 
CCGGTTTATG TTTTTGATGG GAAGCCACCT CAGCTCAAGT CAGGAGAGCT TGAGAAGAGA 
GTTGAGAGAC GAGCAGAGGC TGAGAAACTT CTAGCTCAGG CACAAGAAGC AGGTGAGCAG 
GAAAATATTG ACAAGTTTAG TAAACGACTA GTGAAGGTAA CCAAACAACA CAATGAGGAA 
TGCAAGAAAT TACTTTCTCT AATGGGAGTC CCATACATTG AGGCTCCATG TGAGGCAGAG 
GCGAGTTGTG CTGCTCTGGT GAAAGCAGGG AAGGTTTATG CCACAGCAAC AGAAGATATG 
GACGGTCTGA CATTTGGAAC AACGGTCTTA TTGAGGCATC TAACAGCTAG TGAAGCAAAA 
AAGCTCCCCA TCCAAGAGTT TCACTTCAGT CGTATTCTGC AGGATATGGA GCTGACGCAT 
CAACAGTTCA TCGACCTGTG TATCCTGCTG GGCTGTGACT ACTGTGGCAC TATAAAGGGA 
ATCGGACCCA AGAGGGCCAT TGACCTAATT AAACAGCATG GCTCTATTGA AGAAATTCTG 
GAGAATATTG ACCCAAATAA GCACCCTGCT CCAGAGGACT GGCTGTATAA AGAGGCTCGG 
GGTCTCTTCT TGGAACCTGA GGTCGTGGAT GGCACCTCTG TCGATCTGAA GTGGAATGAA 
CCTGATGAGG ATGGGCTAAT TCAGTTCATG TGTGCTGAGA AGCAGTTCAG TGAGGATCGT 
ATCCGTAACG GCTGTAAGAA GATCACAAAG AGCAGACAAG GCAGCACCCA GGGCCGACTG 
GACACATTCT TTACTGTAAC CGGATCAATC TCTTCTAAGC GTAAGGAGCC TGAAACAAAG 
GGATCTGCCA AAAAGAAACA AAAGACAAGT GCTACGCCAG GCAAATTTAA GAAGGGCAAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Oxidative stress, cell cycle arrest, DNA damage and apoptosis in adult zebrafish (Danio rerio) induced by tris(1,3-dichloro-2-propyl) phosphate.
Aquatic toxicology (Amsterdam, Netherlands)19437-45(2018 Jan)
Chen H,Wang P,Du Z,Wang G,Gao S

Effects of starch-coating of magnetite nanoparticles on cellular uptake, toxicity and gene expression profiles in adult zebrafish.
The Science of the total environment622-623930-941(2017 Dec)
Zheng M,Lu J,Zhao D

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

TPhP exposure disturbs carbohydrate metabolism, lipid metabolism, and the DNA damage repair system in zebrafish liver.
Scientific reports621827(2016 Feb)
Du Z,Zhang Y,Wang G,Peng J,Wang Z,Gao S

Epigenetic Studies Point to DNA Replication/Repair Genes as a Basis for the Heritable Nature of Long Term Complications in Diabetes.
Journal of diabetes research20162860780(2016)
Leontovich AA,Intine RV,Sarras MP Jr