tob1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tob1a
Entrez Gene ID	386629
Full Name	transducer of ERBB2, 1a
Synonyms	tob1
General protein information	Preferred Names transducer of ERBB2, 1a Names protein Tob1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_198363.1	NP_938177.1	protein Tob1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Tob1	NP_033453.2
Rattus norvegicus (Norway rat)	Tob1	NP_579851.2
Xenopus tropicalis (tropical clawed frog)	tob1	NP_001016611.1
Homo sapiens (human)	TOB1	NP_001230806.1
Danio rerio (zebrafish)	tob1b	NP_998139.1
Pan troglodytes (chimpanzee)	TOB1	XP_523795.3
	LOC706873	XP_001095282.1
Canis lupus familiaris (dog)	TOB1	XP_005624733.1
Danio rerio (zebrafish)	tob1a	NP_938177.1
Bos taurus (cattle)	TOB1	NP_001070543.2
Gallus gallus (chicken)	TOB1	NP_001001467.1

Gene Ontology
Bibliography

Related articles in PubMed

Zebrafish Collagen Type I: Molecular and Biochemical Characterization of the Major Structural Protein in Bone and Skin.
Gistelinck C, Gioia R, Gagliardi A, Tonelli F, Marchese L, Bianchi L, Landi C, Bini L, Huysseune A, Witten PE, Staes A, Gevaert K, De Rocker N, Menten B, Malfait F, Leikin S, Carra S, Tenni R, Rossi A, De Paepe A, Coucke P, Willaert A, Forlino A
Scientific reports621540(2016 Feb)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Zebrafish kidney marrow contains ABCG2-dependent side population cells exhibiting hematopoietic stem cell properties.
Tsinkalovsky O, Vik-Mo AO, Ferreira S, Laerum OD, Fjose A
Differentiation; research in biological diversity75(3)175-83(2007 Mar)

Tob1 controls dorsal development of zebrafish embryos by antagonizing maternal beta-catenin transcriptional activity.
Xiong B, Rui Y, Zhang M, Shi K, Jia S, Tian T, Yin K, Huang H, Lin S, Zhao X, Chen Y, Chen YG, Lin SC, Meng A
Developmental cell11(2)225-38(2006 Aug)

The zebrafish gene map defines ancestral vertebrate chromosomes.
Woods IG, Wilson C, Friedlander B, Chang P, Reyes DK, Nix R, Kelly PD, Chu F, Postlethwait JH, Talbot WS
Genome research15(9)1307-14(2005 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following tob1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tob1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa31827	NM_198363.1 Latest version!	Danio rerio transducer of ERBB2, 1a (tob1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tob1a ( NM_198363.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_938177.1 Danio rerio transducer of ERBB2, 1a (tob1a), mRNA.

Price & Availability↑

CloneID	ODa31827
Clone ID Related Accession (Same CDS sequence)	NM_198363.1
Accession Version	NM_198363.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 969bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	protein Tob1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC056290.1. ##Evidence-Data-START## Transcript exon combination :: BC056290.1, BC066569.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGCAGCTTG AAATCCAAGT AGCACTCAAC TTCATCATTT CGTACTTGTA CAACAAGCTG 
CCCAGGCGAC GTGTCAACAT CTTCGGCGAG GAGTTAGAGC GACAGCTGAA ACAGAAATAC 
GAGGGACACT GGTACCCGGA CAAGCCATAC AAAGGGTCTG GATTCAGGTG TATACACGTT 
GGGGAGAAAG TGGACCCAGT TGTAGAGCAA GCAGCCAAAG AAAGTGGGTT GGACATTGAG 
GATGTCCGCA ACAACCTGCC TCAGGACCTC AGCGTCTGGA TCGATCCTTT CGAGGTGTCT 
TATCAGATTG GGGAGAAGGG ACCCGTCAAG GTGCTCTATG TGGATGACAG TAACGATAAC 
GGGCTGGAGT TGGACAAGGA GATCAAGAAC AGCTTTAACC CCGAGGCGCA GGTCTTCATG 
CCTATTAGCG AGCCTGTCGG CATGTCCCCC ACCTCCAGTT CCCCGTCCCC ACCGTTCGGC 
CAGTCGGCCG CAGTCAGCCC GACGTTTATG CGCCGTTCCA CGCAACCTCT AACCTTCACC 
ACTGCCACCT TCGCCGCCAC TAAATTTGGC TCCACGAAAA TGAAAAGCAA TGGGCGCAAC 
GCCAATAAGG TGACCCGAAA CTCCCCCACC AACCTGGGCC TGAATGTAAA CAACCTCCTG 
AAGCAGAAAG CCATGTCCAC ATCGATGCAT TCTCTATACG GCGTCGGCGC TCAGCCGCAG 
AAGCCCTCTG CCCTGTCTCC GAACGCCAAG GAGTTTGTCT TTCCCAATCT CCAGGGGCAG 
GGCAGCCCAA GTGCAATGTT CCCCGGGGAA ACGTCCCTCA ATCTCAGCCC TCTCCCCTAC 
AATAATGCCT TCGACGTGTT TGCAGCCTAT GGGGGCCTTA ATGAGAAGTC CCTCATGGAC 
AGTCTGAATT TCAGCTTGAG CAACATGCAG TATTCTAACC AGCAATTCCA GCCAGTGATG 
GCCAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_938177.1
CDS	296..1264
Translation	MQLEIQVALNFIISYLYNKLPRRRVNIFGEELERQLKQKYEGHWYPDKPYKGSGFRCIHVGEKVDPVVEQAAKESGLDIEDVRNNLPQDLSVWIDPFEVSYQIGEKGPVKVLYVDDSNDNGLELDKEIKNSFNPEAQVFMPISEPVGMSPTSSSPSPPFGQSAAVSPTFMRRSTQPLTFTTATFAATKFGSTKMKSNGRNANKVTRNSPTNLGLNVNNLLKQKAMSTSMHSLYGVGAQPQKPSALSPNAKEFVFPNLQGQGSPSAMFPGETSLNLSPLPYNNAFDVFAAYGGLNEKSLMDSLNFSLSNMQYSNQQFQPVMAN

Target ORF information:

RefSeq Version	NM_198363.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transducer of ERBB2, 1a (tob1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198363.1

ORF Insert Sequence:

ATGCAGCTTG AAATCCAAGT AGCACTCAAC TTCATCATTT CGTACTTGTA CAACAAGCTG 
CCCAGGCGAC GTGTCAACAT CTTCGGCGAG GAGTTAGAGC GACAGCTGAA ACAGAAATAC 
GAGGGACACT GGTACCCGGA CAAGCCATAC AAAGGGTCTG GATTCAGGTG TATACACGTT 
GGGGAGAAAG TGGACCCAGT TGTAGAGCAA GCAGCCAAAG AAAGTGGGTT GGACATTGAG 
GATGTCCGCA ACAACCTGCC TCAGGACCTC AGCGTCTGGA TCGATCCTTT CGAGGTGTCT 
TATCAGATTG GGGAGAAGGG ACCCGTCAAG GTGCTCTATG TGGATGACAG TAACGATAAC 
GGGCTGGAGT TGGACAAGGA GATCAAGAAC AGCTTTAACC CCGAGGCGCA GGTCTTCATG 
CCTATTAGCG AGCCTGTCGG CATGTCCCCC ACCTCCAGTT CCCCGTCCCC ACCGTTCGGC 
CAGTCGGCCG CAGTCAGCCC GACGTTTATG CGCCGTTCCA CGCAACCTCT AACCTTCACC 
ACTGCCACCT TCGCCGCCAC TAAATTTGGC TCCACGAAAA TGAAAAGCAA TGGGCGCAAC 
GCCAATAAGG TGACCCGAAA CTCCCCCACC AACCTGGGCC TGAATGTAAA CAACCTCCTG 
AAGCAGAAAG CCATGTCCAC ATCGATGCAT TCTCTATACG GCGTCGGCGC TCAGCCGCAG 
AAGCCCTCTG CCCTGTCTCC GAACGCCAAG GAGTTTGTCT TTCCCAATCT CCAGGGGCAG 
GGCAGCCCAA GTGCAATGTT CCCCGGGGAA ACGTCCCTCA ATCTCAGCCC TCTCCCCTAC 
AATAATGCCT TCGACGTGTT TGCAGCCTAT GGGGGCCTTA ATGAGAAGTC CCTCATGGAC 
AGTCTGAATT TCAGCTTGAG CAACATGCAG TATTCTAACC AGCAATTCCA GCCAGTGATG 
GCCAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Zebrafish Collagen Type I: Molecular and Biochemical Characterization of the Major Structural Protein in Bone and Skin.
Scientific reports621540(2016 Feb)
Gistelinck C,Gioia R,Gagliardi A,Tonelli F,Marchese L,Bianchi L,Landi C,Bini L,Huysseune A,Witten PE,Staes A,Gevaert K,De Rocker N,Menten B,Malfait F,Leikin S,Carra S,Tenni R,Rossi A,De Paepe A,Coucke P,Willaert A,Forlino A

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Zebrafish kidney marrow contains ABCG2-dependent side population cells exhibiting hematopoietic stem cell properties.
Differentiation; research in biological diversity75(3)175-83(2007 Mar)
Tsinkalovsky O,Vik-Mo AO,Ferreira S,Laerum OD,Fjose A

Tob1 controls dorsal development of zebrafish embryos by antagonizing maternal beta-catenin transcriptional activity.
Developmental cell11(2)225-38(2006 Aug)
Xiong B,Rui Y,Zhang M,Shi K,Jia S,Tian T,Yin K,Huang H,Lin S,Zhao X,Chen Y,Chen YG,Lin SC,Meng A

The zebrafish gene map defines ancestral vertebrate chromosomes.
Genome research15(9)1307-14(2005 Sep)
Woods IG,Wilson C,Friedlander B,Chang P,Reyes DK,Nix R,Kelly PD,Chu F,Postlethwait JH,Talbot WS

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tob1a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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