ncf1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ncf1
Entrez Gene ID	378966
Full Name	neutrophil cytosolic factor 1
Synonyms	cb893
General protein information	Preferred Names neutrophil cytosolic factor 1 Names neutrophil cytosol factor 1 p47phox
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001030071.2	NP_001025242.1	neutrophil cytosol factor 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	NCF1	NP_000256.4
Canis lupus familiaris (dog)	NCF1	XP_849574.2
Mus musculus (house mouse)	Ncf1	NP_001272966.1
Rattus norvegicus (Norway rat)	Ncf1	NP_446186.2
Xenopus tropicalis (tropical clawed frog)	ncf1	NP_001106375.1
	NCF1	XP_001084055.1
Gallus gallus (chicken)	NCF1	NP_001025880.1
Pan troglodytes (chimpanzee)	NCF1	XP_001152639.1
Bos taurus (cattle)	NCF1	NP_776544.1
Danio rerio (zebrafish)	ncf1	NP_001025242.1

Gene Ontology
Bibliography

Related articles in PubMed

Complement Receptor C5aR1 Plays an Evolutionarily Conserved Role in Successful Cardiac Regeneration.
Natarajan N, Abbas Y, Bryant DM, Gonzalez-Rosa JM, Sharpe M, Uygur A, Cocco-Delgado LH, Ho NN, Gerard NP, Gerard CJ, Macrae CA, Burns CE, Burns CG, Whited JL, Lee RT
Circulation(2018 Jan)

Targeted Gene Expression in Zebrafish Exposed to Chlorpyrifos-Oxon Confirms Phenotype-Specific Mechanisms Leading to Adverse Outcomes.
Garcia-Reyero N, Escalon L, Prats E, Faria M, Soares AM, Rald?a D
Bulletin of environmental contamination and toxicology96(6)707-13(2016 Jun)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Telomerase Is Essential for Zebrafish Heart Regeneration.
Bednarek D, Gonz?lez-Rosa JM, Guzm?n-Mart?nez G, Guti?rrez-Guti?rrez ?, Aguado T, S?nchez-Ferrer C, Marques IJ, Galardi-Castilla M, de Diego I, G?mez MJ, Cort?s A, Zapata A, Jim?nez-Borreguero LJ, Mercader N, Flores I
Cell reports12(10)1691-703(2015 Sep)

Incomplete splicing of neutrophil-specific genes affects neutrophil development in a zebrafish model of poikiloderma with neutropenia.
Patil P, Uechi T, Kenmochi N
RNA biology12(4)426-34(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ncf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ncf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa01746	NM_001030071.2 Latest version!	Danio rerio neutrophil cytosolic factor 1 (ncf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ncf1 ( NM_001030071.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001025242.1 Danio rerio neutrophil cytosolic factor 1 (ncf1), mRNA.

Price & Availability↑

CloneID	ODa01746
Clone ID Related Accession (Same CDS sequence)	NM_001030071.2
Accession Version	NM_001030071.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	neutrophil cytosol factor 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY972158.1. On Aug 31, 2012 this sequence version replaced NM_001030071.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY972158.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCTGAAA CATACGTGAG ACATGTGGAA CTGCTTGGAT TTGAGAAAAG ATTCTTTCCC 
AGCCAGCACT ATGTGTACAT GCTCCTGGTT AAATGGAGCG ATCAGTCTGA AAAGCTGGTG 
TACAGACGCT ATCCAGAAGT ACACACATTA CATAAAACTC TGAAGGAAAT GTTTCCTATT 
GAGGCAGGAG ATATTGACGA AAAGGACCGA ATCATTCCCA CATTACCAGC TCCTAAATGG 
CTGGACAATC AGAAAACCAC AGAGACGAGG CAGGCGACTC TTGCTGAATA TTGCCGATCT 
CTTCTCAACC TGCCCGCCAA CATCTCCCGC TGTCAGCTCA TTCGGGACTT CTTCAAAATG 
AGGCCTGAAG ACGAAACTCC ACCAGCACCA CATCCATACA AAAGAAATGA GACCTTCATT 
ATGTCCACAA ACAGAGTACG GAGCAACACC ACATCAGAAA TCACAGGGCC CATCATGCTC 
GAGACCTACA GAGTGATCGC AGACTACAGC AAGAGCTCCA AGTATGAGCT CACGCTGAAG 
ATGGGAGACA TGGTGGACAT CGTGGAGAAA AGCCCAAATG GCTGGTGGTT TTGTCAGTGT 
GAGTCCAGGA GAGGATGGGT TCCAGCTTCA TACTTAGAGC CGCTTGATGG AGCAGATGAA 
TCTGAGGAAC CAGAGCCTAA TTATGCAGGA GAGCTCTACA AAACCACCAG AGGCTATAAA 
GCTGTGGAAC AGGATGAGAT GACTCTCGAA GCAGGAGTCA TTATTGAAGT CATCCATAAA 
CTTCTTGATG GGTGGTGGGT GGTCAGGAAA GGCGAGGAAA CCGGCTTCTA TCCGTCCATG 
TTCCTGTGCA GGACGGGGGA GAAGAAAGAG GTGGATGCTG AGAGGGACGT GGTCAGAAGA 
GCAACACCAC CACCAAGAAG GTCGACCATC CGCAATGCCC AGAGTATCCA TTCTACGGTA 
CGCCGGCGAA TCAGTCAGGA CAGCTACCGG AAACAGAGCC GTCGCTTCTT ACAGCAGAGA 
GGAAGACTGA ACTCTCATTC CAGGATCGGG ACACGATCTC CACTGCAGGA GAGGAGGACA 
AACAGAGAGA ACATCGAGAA ATCCAGCGCT CCTCAAGCAG AAGATGAGGA TAAAAGTGTT 
CCGGTGGTTC CTCCTCGGCC CAGTCCTCAG CTCATTCTGG AGCGCTGCAC TGAAAACACA 
AGCAAGAGGA TGAGCATGCA AGAGGCTGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001025242.1
CDS	56..1288
Translation	MAETYVRHVELLGFEKRFFPSQHYVYMLLVKWSDQSEKLVYRRYPEVHTLHKTLKEMFPIEAGDIDEKDRIIPTLPAPKWLDNQKTTETRQATLAEYCRSLLNLPANISRCQLIRDFFKMRPEDETPPAPHPYKRNETFIMSTNRVRSNTTSEITGPIMLETYRVIADYSKSSKYELTLKMGDMVDIVEKSPNGWWFCQCESRRGWVPASYLEPLDGADESEEPEPNYAGELYKTTRGYKAVEQDEMTLEAGVIIEVIHKLLDGWWVVRKGEETGFYPSMFLCRTGEKKEVDAERDVVRRATPPPRRSTIRNAQSIHSTVRRRISQDSYRKQSRRFLQQRGRLNSHSRIGTRSPLQERRTNRENIEKSSAPQAEDEDKSVPVVPPRPSPQLILERCTENTSKRMSMQEAD

Target ORF information:

RefSeq Version	NM_001030071.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neutrophil cytosolic factor 1 (ncf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001030071.2

ORF Insert Sequence:

ATGGCTGAAA CATACGTGAG ACATGTGGAA CTGCTTGGAT TTGAGAAAAG ATTCTTTCCC 
AGCCAGCACT ATGTGTACAT GCTCCTGGTT AAATGGAGCG ATCAGTCTGA AAAGCTGGTG 
TACAGACGCT ATCCAGAAGT ACACACATTA CATAAAACTC TGAAGGAAAT GTTTCCTATT 
GAGGCAGGAG ATATTGACGA AAAGGACCGA ATCATTCCCA CATTACCAGC TCCTAAATGG 
CTGGACAATC AGAAAACCAC AGAGACGAGG CAGGCGACTC TTGCTGAATA TTGCCGATCT 
CTTCTCAACC TGCCCGCCAA CATCTCCCGC TGTCAGCTCA TTCGGGACTT CTTCAAAATG 
AGGCCTGAAG ACGAAACTCC ACCAGCACCA CATCCATACA AAAGAAATGA GACCTTCATT 
ATGTCCACAA ACAGAGTACG GAGCAACACC ACATCAGAAA TCACAGGGCC CATCATGCTC 
GAGACCTACA GAGTGATCGC AGACTACAGC AAGAGCTCCA AGTATGAGCT CACGCTGAAG 
ATGGGAGACA TGGTGGACAT CGTGGAGAAA AGCCCAAATG GCTGGTGGTT TTGTCAGTGT 
GAGTCCAGGA GAGGATGGGT TCCAGCTTCA TACTTAGAGC CGCTTGATGG AGCAGATGAA 
TCTGAGGAAC CAGAGCCTAA TTATGCAGGA GAGCTCTACA AAACCACCAG AGGCTATAAA 
GCTGTGGAAC AGGATGAGAT GACTCTCGAA GCAGGAGTCA TTATTGAAGT CATCCATAAA 
CTTCTTGATG GGTGGTGGGT GGTCAGGAAA GGCGAGGAAA CCGGCTTCTA TCCGTCCATG 
TTCCTGTGCA GGACGGGGGA GAAGAAAGAG GTGGATGCTG AGAGGGACGT GGTCAGAAGA 
GCAACACCAC CACCAAGAAG GTCGACCATC CGCAATGCCC AGAGTATCCA TTCTACGGTA 
CGCCGGCGAA TCAGTCAGGA CAGCTACCGG AAACAGAGCC GTCGCTTCTT ACAGCAGAGA 
GGAAGACTGA ACTCTCATTC CAGGATCGGG ACACGATCTC CACTGCAGGA GAGGAGGACA 
AACAGAGAGA ACATCGAGAA ATCCAGCGCT CCTCAAGCAG AAGATGAGGA TAAAAGTGTT 
CCGGTGGTTC CTCCTCGGCC CAGTCCTCAG CTCATTCTGG AGCGCTGCAC TGAAAACACA 
AGCAAGAGGA TGAGCATGCA AGAGGCTGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Complement Receptor C5aR1 Plays an Evolutionarily Conserved Role in Successful Cardiac Regeneration.
Circulation(2018 Jan)
Natarajan N,Abbas Y,Bryant DM,Gonzalez-Rosa JM,Sharpe M,Uygur A,Cocco-Delgado LH,Ho NN,Gerard NP,Gerard CJ,Macrae CA,Burns CE,Burns CG,Whited JL,Lee RT

Targeted Gene Expression in Zebrafish Exposed to Chlorpyrifos-Oxon Confirms Phenotype-Specific Mechanisms Leading to Adverse Outcomes.
Bulletin of environmental contamination and toxicology96(6)707-13(2016 Jun)
Garcia-Reyero N,Escalon L,Prats E,Faria M,Soares AM,Rald?a D

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Telomerase Is Essential for Zebrafish Heart Regeneration.
Cell reports12(10)1691-703(2015 Sep)
Bednarek D,Gonz?lez-Rosa JM,Guzm?n-Mart?nez G,Guti?rrez-Guti?rrez ?,Aguado T,S?nchez-Ferrer C,Marques IJ,Galardi-Castilla M,de Diego I,G?mez MJ,Cort?s A,Zapata A,Jim?nez-Borreguero LJ,Mercader N,Flores I

Incomplete splicing of neutrophil-specific genes affects neutrophil development in a zebrafish model of poikiloderma with neutropenia.
RNA biology12(4)426-34(2015)
Patil P,Uechi T,Kenmochi N