ptf1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ptf1a
Entrez Gene ID	368662
Full Name	pancreas specific transcription factor, 1a
Synonyms	PTF1,si:zc142h2.2,zgc:112216
General protein information	Preferred Names pancreas specific transcription factor, 1a Names pancreas transcription factor 1 subunit alpha Ptfa bHLH transcription factor p48
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_207641.2	NP_997524.1	pancreas transcription factor 1 subunit alpha

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	ptf1a	NP_997524.1
Bos taurus (cattle)	PTF1A	XP_002707767.1
Mus musculus (house mouse)	Ptf1a	NP_061279.2
Xenopus tropicalis (tropical clawed frog)	ptf1a	XP_002933181.2
Homo sapiens (human)	PTF1A	NP_835455.1
Rattus norvegicus (Norway rat)	Ptf1a	NP_446416.1
Pan troglodytes (chimpanzee)	PTF1A	XP_001146416.2
Canis lupus familiaris (dog)	PTF1A	XP_850386.2

Gene Ontology
Bibliography

Related articles in PubMed

Embryonic exposure to Mono(2-ethylhexyl) phthalate (MEHP) disrupts pancreatic organogenesis in zebrafish (Danio rerio).
Jacobs HM, Sant KE, Basnet A, Williams LM, Moss JB, Timme-Laragy AR
Chemosphere195498-507(2018 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results highlight the possibility that Ptf1a may act as an important transcription factor for enteric neuron development.
Title: A novel subset of enteric neurons revealed by ptf1a:GFP in the developing zebrafish enteric nervous system.

In ptf1a haploinsufficiency, more ptf1a-lineage-labeled cells are traced into the pancreatic Notch-responsive cell and endocrine compartments. More reduction of ptf1a gene dosage converts pancreatic progenitor cells to non-pancreatic cell fates.
Title: Fate mapping of ptf1a-expressing cells during pancreatic organogenesis and regeneration in zebrafish.

Autoregulation of ptf1a is absolutely required for exocrine pancreas formation.
Title: Distinct enhancers of ptf1a mediate specification and expansion of ventral pancreas in zebrafish.

Postembryonic antagonism of Ptf1a, a master regulator of pancreatic development and acinar cell fate specification, induced expression of endocrine genes including insulin in the exocrine compartment. Induced insulin+ cells are derived from acinar cells.
Title: Suppression of Ptf1a activity induces acinar-to-endocrine conversion.

The timing of Ptf1a expression suggests that it is involved in the very early stages or steps in the differentiation of amacrine cells, which can be seen to rapidly diversify into a large number of subtypes.
Title: Ptf1a is expressed transiently in all types of amacrine cells in the embryonic zebrafish retina.

The following ptf1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ptf1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa00141	NM_207641.2 Latest version!	Danio rerio pancreas associated transcription factor 1a (ptf1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ptf1a ( NM_207641.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_997524.1 Danio rerio pancreas associated transcription factor 1a (ptf1a), mRNA.

Price & Availability↑

CloneID	ODa00141
Clone ID Related Accession (Same CDS sequence)	NM_207641.2
Accession Version	NM_207641.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-09
Organism	Danio rerio(zebrafish)
Product	pancreas transcription factor 1 subunit alpha
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC093269.1. On or before Mar 11, 2007 this sequence version replaced XM_693282.2, NM_207641.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC093269.1, AY245546.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168448, SAMEA3505370 [ECO:0000348] ##Evidence-Data-END##

ATGGACACTG TGTTGGATCC ATTCACAGGC TTAGACTCTT TCTCCTCCTC TTATTTCGAC 
GACGATGACT TCTTTACGGA CCACTCTTCG AGAGACCACT TGGACACAGA CGACTTTCTG 
GACGAGGATG TCGATTTTCT TACCAACCAA ATTCAAGAAT ATTACAAGGA TAGTCGGATA 
TCACAGGACG GAGATTATTG TGACGTTGGC AACTTCTCCT TTTCCTCGTC ATCTTCGACT 
TTTTCTTACG GATGCGCGGA CAGCACCTCG GAGCTGTCCC CTCACAGAGA CGGAGGCTTA 
CTGAAGAGGC GGAGGCGCAT GAGGTCTGAA GTGGAGATGC AACAGTTGCG TCAAGCTGCC 
AACGTCCGGG AGCGACGGAG GATGCAGTCC ATTAACGATG CTTTCGAGGG ACTGCGATCT 
CACATCCCAA CACTGCCATA CGAGAAGAGA CTTTCAAAAG TTGACACTTT ACGCCTAGCA 
ATTGGCTACA TAAACTTTCT TGCGGAACTG GTGCAGTCGG ACATGCCAAT TCGGAACCCA 
CACAGTGACG CCTTAAACCA ACCGAAAAAA GTCATCATTT GCCACCGAGG AACAAGATCC 
CCATCTCCAA ATGACCCAGA CTACGGGTTG CCTCCTCTCG CAGGACACTC TCTTTCATGG 
ACTGATGAGA AGCAGTTAAA AGACCAGAAT ATCATTCGGA CAGCGAAAGT CTGGACACCA 
GAGGACCCAC GAAAGTTGCA CTTGAAATCA TCAATAAACA ATATTGAGAA TGAACCCCCC 
TTTAATTTCA TTTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997524.1
CDS	89..886
Translation	MDTVLDPFTGLDSFSSSYFDDDDFFTDHSSRDHLDTDDFLDEDVDFLTNQIQEYYKDSRISQDGDYCDVGNFSFSSSSSTFSYGCADSTSELSPHRDGGLLKRRRRMRSEVEMQQLRQAANVRERRRMQSINDAFEGLRSHIPTLPYEKRLSKVDTLRLAIGYINFLAELVQSDMPIRNPHSDALNQPKKVIICHRGTRSPSPNDPDYGLPPLAGHSLSWTDEKQLKDQNIIRTAKVWTPEDPRKLHLKSSINNIENEPPFNFIS

Target ORF information:

RefSeq Version	NM_207641.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio pancreas associated transcription factor 1a (ptf1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_207641.2

ORF Insert Sequence:

ATGGACACTG TGTTGGATCC ATTCACAGGC TTAGACTCTT TCTCCTCCTC TTATTTCGAC 
GACGATGACT TCTTTACGGA CCACTCTTCG AGAGACCACT TGGACACAGA CGACTTTCTG 
GACGAGGATG TCGATTTTCT TACCAACCAA ATTCAAGAAT ATTACAAGGA TAGTCGGATA 
TCACAGGACG GAGATTATTG TGACGTTGGC AACTTCTCCT TTTCCTCGTC ATCTTCGACT 
TTTTCTTACG GATGCGCGGA CAGCACCTCG GAGCTGTCCC CTCACAGAGA CGGAGGCTTA 
CTGAAGAGGC GGAGGCGCAT GAGGTCTGAA GTGGAGATGC AACAGTTGCG TCAAGCTGCC 
AACGTCCGGG AGCGACGGAG GATGCAGTCC ATTAACGATG CTTTCGAGGG ACTGCGATCT 
CACATCCCAA CACTGCCATA CGAGAAGAGA CTTTCAAAAG TTGACACTTT ACGCCTAGCA 
ATTGGCTACA TAAACTTTCT TGCGGAACTG GTGCAGTCGG ACATGCCAAT TCGGAACCCA 
CACAGTGACG CCTTAAACCA ACCGAAAAAA GTCATCATTT GCCACCGAGG AACAAGATCC 
CCATCTCCAA ATGACCCAGA CTACGGGTTG CCTCCTCTCG CAGGACACTC TCTTTCATGG 
ACTGATGAGA AGCAGTTAAA AGACCAGAAT ATCATTCGGA CAGCGAAAGT CTGGACACCA 
GAGGACCCAC GAAAGTTGCA CTTGAAATCA TCAATAAACA ATATTGAGAA TGAACCCCCC 
TTTAATTTCA TTTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Embryonic exposure to Mono(2-ethylhexyl) phthalate (MEHP) disrupts pancreatic organogenesis in zebrafish (Danio rerio).
Chemosphere195498-507(2018 Mar)
Jacobs HM,Sant KE,Basnet A,Williams LM,Moss JB,Timme-Laragy AR