itsn1 cDNA ORF clone, Danio rerio(zebrafish)

The following itsn1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the itsn1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa01709 NM_207182.1
Latest version!
Danio rerio intersectin 1 (SH3 domain protein) (itsn1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
ODa06106 XM_005170971.4
Latest version!
Danio rerio intersectin 1 (SH3 domain protein) (itsn1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
ODa06107 XM_005170972.4
Latest version!
Danio rerio intersectin 1 (SH3 domain protein) (itsn1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
ODa06109 XM_005170974.4
Latest version!
Danio rerio intersectin 1 (SH3 domain protein) (itsn1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
View Old Accession Versions >>
ODa06109 XM_005170974.3 Danio rerio intersectin 1 (SH3 domain protein) (itsn1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
ODa06106 XM_005170971.3 Danio rerio intersectin 1 (SH3 domain protein) (itsn1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
ODa06107 XM_005170972.3 Danio rerio intersectin 1 (SH3 domain protein) (itsn1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa01709
    Clone ID Related Accession (Same CDS sequence) NM_207182.1
    Accession Version NM_207182.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5166bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-26
    Organism Danio rerio(zebrafish)
    Product intersectin-1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL606751.5. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGCTCAGT TTCCCACACC CTTCGGAGGG GGGTCGGATA CATGGGTAAT ATCTGTGGAC 
    GAGAGAGCCA AACATGACCA GCAGTTTCAC AGTCTCACCC CAACACCCGC TGGCTTCATC
    ACAGGTGACC AGGCAAGGAA TTTCTTCCTG CAGTCAGGTT TACCTGCCCC CATCTTGGCC
    CAGATATGGG CTCTGGCCGA CATGAACAAT GACGGGAAGA TGGACATGCA TGAGTTTTCC
    ATCGCAATGA AGCTGATTAA ATTAAAGCTG CAGGGTCACC CTCTTCCTCC ATCTCTGCCC
    CCCTCCATGA AGCAACAGCC CATCTCCATA CCCCCGCCGA CCCCTTTTGG AGTGCCAGGG
    ATGGGTGTTT CTCCTCCACT GGGCGCATCG GTAACCCCAC CCGTACCATC CCTCGCCAAC
    GGAGCACCCG CAATGATACA ACCCCTGAGT GGATTTTCAC ATCCAGGTGC CGTGCTCAAC
    AAAACCCCCT CATTCAATCG CTCCAGTATC AAGCTACAGA AGGGCCAGTC TGTAGAGGCG
    CCCAGTGCTC CAGCTGCTCC TCCACCCGCT GACTGGGCCG TGTCTCAGTC CTCCAGACTT
    AAGTATCGCC AGCTCTTTAA CAGCCATGAT AAAATGATGA GCGGTTATCT CACAGGTCCA
    CAAGCCAGAA CTATTCTGAT GCAGTCCAGT CTACCACAAG CCCAGCTGGC CACCATCTGG
    AATCTGTCAG ATATAGATCA GGACGGGAAG TTGACTGCTG AAGAGTTTAT TTTGGCCATG
    CACTTGATCG ACATGGCCAT GTCTGGTCTC CCTCTTCCCC CTTTGCTGCC GCCTGACCTT
    ATACCTCCAA CATTCAGGAG AATGCGCTCT GGTAGTGGAG TGTCTGTGAC CAGCATGCAC
    TCGACAGACT TGCGGTCTCA GGAAGAGCCA GAGGAGGAGG AGAAAGAGGC CGAGAAAAAA
    CTTCCAGTCA CATTTGAGGA TAAGAAACGA GAGAACTTTG AGCGCGGAAA CCTGGAGTTG
    GAGAAACGTC GACAGGCACT TCTGGAGCAG CAGAGGAAAG AGCAGGAGCG ACTGGCGGCG
    CTGGAGAAAG AAGAGCAGGA GCGGAAGGAG AGGGAACGAC TGGAGCAGGA GAGACGCAGA
    CAGCAGGAGC TGGACAAACA GCTGGAGAGA CAGAGGGAGC TGGAGCGACA GCGGGAGGAG
    GAGAGACGCA AGGAGATCGA GAGAAGAGAG GCTGCTAAAC GGGAGCTGGA GCGACAGAGG
    CAGCTGGAGT GGGAGAGGAC GCGCAGACAG GAGCTGCTCA CCCAAAGGAA CAGAGAACAG
    GAGAACATCG TCCTGCTTAA AGCACGGAAG AAAACTCTCG AGTTTGAGCT GGAGGCTCTG
    AATGATAAGA AGTCTCAGTT GGAGGGGAAA CTGCAGGATA TTCGGTCCCG TCTCTCGATT
    CAGAGGCATG AGATCGAGAC CACCAATAAG ACTCGGGAGC TCCGCATTGC AGAGATCACA
    AGCCTGCAAC AGCAGCTGCA GGAGTCTCAG CAGTGGTTGA GTCGCTTGAT TCCTGATAAA
    CAGTGTCTGA ATGACCAGCT GAAGCAGGTT CAGCAGAACA GTCTGCACAG AGACTCTTTG
    AACAGTCTTC AGAGGGCCAT TGAAATGAAA GAATCCACCA AACAGCAGCT GAAAGAACAG
    CTGGATGCTG TGGAGAAGGA AACTCGCTCC AAACTATTGG AGATCGACGC CTTCAACACA
    CAGCTGAAGG AGCTGAGAGA AATCCACAAC AAACAGCAGA GGCAGAAACA GAAGGAGCTG
    GAGGCTGACA TCACCCAGCC GTCACATGAC AGGAAGTCCA TAGACTCACA AGACTCGAGG
    TTGTCGGGGA CTGATGATGG CGTGTCTCCA GCGTGGAGGG ATGATGGGCT GGGTAAAGCT
    CCTACTCCTC CTGTGTCTCA GGCCTGGATG AGCCGGGTGA GAGAAAAGAA GATTGTCGAT
    GCAGAAAGAC ATGGATTTGA TCAATTCTGC TGCTGCTTTA TGAGAGTAAA TGTCAGAAAT
    CAACATAAGT CATGTGTTTG TGTTCTAGAT AAGATCCCAG TGTCTGGTTT TAATCAGGAG
    AAGGTAAAGG TGGTCTACTA TAGAGCAATG TATCCCTTTG AGGCCCGCAG TCATGATGAG
    ATCACCATCC ATCCTGGAGA CATAGTCATG GTGGATGAGT CTCAGACAGG TGAGCCTGGA
    TGGTTAGGAG GAGAGATTAA AGGGAAGACT GGCTGGTTTC CTGCCAACTA TGCGGAGAAG
    ATTCCAGAAT CCGAAGTTCC TCTGAGTTTG AGGGCCAGCG CCGCTTCTAG CTCCGCCCCT
    AAATTGGGTT CTCACATGTC ATCTGCTTCT TCATCGACCA CGGCCACGCC CATTCTGCCA
    GTCTCCACAG AGCCTGCATC GATAGCCCCG CCCTCTTCAG CCCCACCCCC CACAGGCCCT
    GCCTCCTCCT CTTCCTCAGC ATCCAGCAAT TGGGCAGATT TCAGCACTAC ATGGCCCTCT
    AATTCTGCTG TGGAGAAGCA GGATAGTGAT GGATGGGATG CATGGCCGAC TCAGCCAACT
    CAGCCTTCTC TGTCAGTGCC CAGTGGAGGG CAGATCAGAC AGCGCTCTGC TTTCACGCCT
    GCTACACTCT CCGGCTCCTC GCCCTCACCC GTGCTTGGAC AGGGTGAGAA GGTGGAGGGT
    CTGCAGGCTC AGGCGCTGTA CCCATGGAGA GCCAAGAAAG ACAATCATCT AAACTTCAAC
    AAGAATGACG TGATCACTGT ACTGGAGCAG CAGGACATGT GGTGGTTTGG GGAGGTGCAA
    GGACAGAGAG GATGGTTCCC TAAATCTTAC GTAAAGCTCA TCTCTGGGCC TGTCCGGAAA
    TCTATGAGTA TTGATTCTGG CTCTTCAGAC AGCCCTCCTA GTGTAAAGAG ACCCAGTCCC
    TCTCTAAACA AACCCACAGA GCTCGGAGAA GGTCAGAACT CCAACAGCAA CTCAATGTAT
    CCGTCTAAAG AGTATGTGGC CATGTATACA TATGAAAGCA ATGAGCAGGG TGACTTGACC
    TTCCAGCAAG GTGATGTCAT CACAGTCACC AAGAAAGAAG GAGATTGGTG GACCGGCACT
    GTGAGTGGGA AGATCGGAGT CTTTCCATCG AATTATGTCA AACCTAAAGA ATCCGAGGGG
    TTGGGATCTG CTGGGAAAAC AGGAAGTTTA GGGAAGAAAC CAGAGATTGC CCAAGTCATC
    GCTCCATACA CGGCCACTGG TGCAGAGCAG CTCACTTTAG CACCAGGCCA GCTCATTCTC
    ATTCGCAAGA AAAACCCGGG AGGATGGTGG GAAGGAGAAC TTCAGGCAAG AGGAAAGAAG
    CGGCAGATTG GCTGGTTTCC TGCTAATTAT GTCAAATTAT TGAGTCCCAG TACCAGTAAG
    ACAACACCCA CAGATCCCAA CCCACCCAAG CTGCCGACAC CCAATGCAGT GTGCCAGGTG
    ATTGGCATGT ACGATTACAC TGCTCAGAAT GATGACGAGC TGCCTTTCGG GAAGGGCCAA
    ATCATCAATG TGTTGAGCAG AGAGGATCCC GATTGGTGGA AAGGAGAGTT GAATGGATCC
    GTCGGCCTCT TCCCCTCGAA CTACGTCAAG CTGACCACCG ACACAGACCC CAGCCAACAA
    TGGTGTGCTG ATCTCCACCT GCTCGACATG CTGACCCCAG TGGAGCGGAA GAGGCAGGGA
    TACATACATG AGCTTATTGT TACAGAGGAG AACTATGTCA ATGACCTGCA GCTGGTCACT
    GAGACCTTCC AGAAGCCTCT GCTGGAGTCT GATTTGTTGA CGGAGAAGGA GGTTGCCATG
    ATCTTTGTCA ACTGGAAAGA GCTGACCATG TGCAACATTA AACTGCTCAA GGCGCTGCGG
    GTGAGGAAGA AGATGTCAGG TGAGCGGATG CCAGTGAAGA TGATTGGTGA CATCCTGACG
    GCTCAGCTTC CTCACATGCA GCCGTACATC CGCTTCTGCA GCTGCCAGCT CAACGGCGCC
    ACCCTCATTC AGCAGAAAAC CGACGAGGTG CCCGAATTCA AGGACTTTGT GAAGAGGTTA
    GCGATGGACC CTCGCTGTAA GGGAATGCCC CTGTCCAGTT TCCTTCTCAA ACCCATGCAG
    AGAGTCACCC GATACCCCCT CATCATCAAA AACATTTTAG AGAACACTCC AGAAAGCCAC
    CCAGACCACA GTCACCTGCG TCTGGCTCTG GAGAAAGCAG AAGAGCTGTG CTCGCAGGTC
    AATGAAGGTG TACGAGAGAA AGAAAACTCC GACCGGCTGG AATGGATACA GGCACATGTG
    CAGTGTGAGG GACTGTCAGA GCAACTTGTG TTCAACTCTG TAACAAACTG TCTGGGCCCT
    CGGAAGTTCC TGCACAGTGG AAAACTCTAC AAAGCCAAGA GCAACAAAGA GCTTTACGGC
    TTCCTTTTTA ACGACTTCCT GCTCCTCACA CAGATAATCA AACCTCTGGG CTCATCCGGG
    ACAGACAAGG TCTTCAGTGC GAAATCCCAT CTGCAGTATC GCATGTACAA AACGCCCATC
    TTTCTCAATG AGGTGCTAGT GAAGCTGCCT ACTGACCCCT CGGGAGATGA ACCCATTTTT
    CACATATCTC ACATCGACAG AGTCTACACT ATACGGGCTG AGAGCATCAA CGAAAGGACA
    GCTTGGGTGC AGAAAATCAA AGCTGCCTCT GAGCTCTTCA TCGAAACCGA AAAGAGGAAG
    AGAGAAAAAG CCTACTTGGT GAGGTCTCAG AGGGCCACTG GCATCGGGCG GCTAATGGTC
    AACATTGTTG AAGGCATCGA ACTGAAGCCC TGCCGCTCAA ATGGTAAGAG TAACCCGTAC
    TGTGAGGTGA CGATGGGCTC TCAGTGTCAC GTCACCAAGA CCCTTCAGGA CACTCTGAAC
    CCCAAGTGGA ACTCAAACTG CCAGTTCTTC ATCAAAGACC TGGAGCAGGA CGTGCTGTGT
    GTCACTGTGT TTGAGAGGGA TCAGTTCTCC CCGGACGATT TCTTGGGCAG GACGGAGATT
    CGGCTGGCCG ACATCAAGAA GGACCAGGGC TCGAAGGGTC CCATCACCAA GCGTCTGCTT
    TTACACGAAG TCCCGACGGG AGAGATTGTG GTGCGACTGG ACCTGCAGCT CTTCGATGAA
    TCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_997065.1
    CDS32..5197
    Translation

    Target ORF information:

    RefSeq Version NM_207182.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio intersectin 1 (SH3 domain protein) (itsn1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_207182.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGCTCAGT TTCCCACACC CTTCGGAGGG GGGTCGGATA CATGGGTAAT ATCTGTGGAC 
    GAGAGAGCCA AACATGACCA GCAGTTTCAC AGTCTCACCC CAACACCCGC TGGCTTCATC
    ACAGGTGACC AGGCAAGGAA TTTCTTCCTG CAGTCAGGTT TACCTGCCCC CATCTTGGCC
    CAGATATGGG CTCTGGCCGA CATGAACAAT GACGGGAAGA TGGACATGCA TGAGTTTTCC
    ATCGCAATGA AGCTGATTAA ATTAAAGCTG CAGGGTCACC CTCTTCCTCC ATCTCTGCCC
    CCCTCCATGA AGCAACAGCC CATCTCCATA CCCCCGCCGA CCCCTTTTGG AGTGCCAGGG
    ATGGGTGTTT CTCCTCCACT GGGCGCATCG GTAACCCCAC CCGTACCATC CCTCGCCAAC
    GGAGCACCCG CAATGATACA ACCCCTGAGT GGATTTTCAC ATCCAGGTGC CGTGCTCAAC
    AAAACCCCCT CATTCAATCG CTCCAGTATC AAGCTACAGA AGGGCCAGTC TGTAGAGGCG
    CCCAGTGCTC CAGCTGCTCC TCCACCCGCT GACTGGGCCG TGTCTCAGTC CTCCAGACTT
    AAGTATCGCC AGCTCTTTAA CAGCCATGAT AAAATGATGA GCGGTTATCT CACAGGTCCA
    CAAGCCAGAA CTATTCTGAT GCAGTCCAGT CTACCACAAG CCCAGCTGGC CACCATCTGG
    AATCTGTCAG ATATAGATCA GGACGGGAAG TTGACTGCTG AAGAGTTTAT TTTGGCCATG
    CACTTGATCG ACATGGCCAT GTCTGGTCTC CCTCTTCCCC CTTTGCTGCC GCCTGACCTT
    ATACCTCCAA CATTCAGGAG AATGCGCTCT GGTAGTGGAG TGTCTGTGAC CAGCATGCAC
    TCGACAGACT TGCGGTCTCA GGAAGAGCCA GAGGAGGAGG AGAAAGAGGC CGAGAAAAAA
    CTTCCAGTCA CATTTGAGGA TAAGAAACGA GAGAACTTTG AGCGCGGAAA CCTGGAGTTG
    GAGAAACGTC GACAGGCACT TCTGGAGCAG CAGAGGAAAG AGCAGGAGCG ACTGGCGGCG
    CTGGAGAAAG AAGAGCAGGA GCGGAAGGAG AGGGAACGAC TGGAGCAGGA GAGACGCAGA
    CAGCAGGAGC TGGACAAACA GCTGGAGAGA CAGAGGGAGC TGGAGCGACA GCGGGAGGAG
    GAGAGACGCA AGGAGATCGA GAGAAGAGAG GCTGCTAAAC GGGAGCTGGA GCGACAGAGG
    CAGCTGGAGT GGGAGAGGAC GCGCAGACAG GAGCTGCTCA CCCAAAGGAA CAGAGAACAG
    GAGAACATCG TCCTGCTTAA AGCACGGAAG AAAACTCTCG AGTTTGAGCT GGAGGCTCTG
    AATGATAAGA AGTCTCAGTT GGAGGGGAAA CTGCAGGATA TTCGGTCCCG TCTCTCGATT
    CAGAGGCATG AGATCGAGAC CACCAATAAG ACTCGGGAGC TCCGCATTGC AGAGATCACA
    AGCCTGCAAC AGCAGCTGCA GGAGTCTCAG CAGTGGTTGA GTCGCTTGAT TCCTGATAAA
    CAGTGTCTGA ATGACCAGCT GAAGCAGGTT CAGCAGAACA GTCTGCACAG AGACTCTTTG
    AACAGTCTTC AGAGGGCCAT TGAAATGAAA GAATCCACCA AACAGCAGCT GAAAGAACAG
    CTGGATGCTG TGGAGAAGGA AACTCGCTCC AAACTATTGG AGATCGACGC CTTCAACACA
    CAGCTGAAGG AGCTGAGAGA AATCCACAAC AAACAGCAGA GGCAGAAACA GAAGGAGCTG
    GAGGCTGACA TCACCCAGCC GTCACATGAC AGGAAGTCCA TAGACTCACA AGACTCGAGG
    TTGTCGGGGA CTGATGATGG CGTGTCTCCA GCGTGGAGGG ATGATGGGCT GGGTAAAGCT
    CCTACTCCTC CTGTGTCTCA GGCCTGGATG AGCCGGGTGA GAGAAAAGAA GATTGTCGAT
    GCAGAAAGAC ATGGATTTGA TCAATTCTGC TGCTGCTTTA TGAGAGTAAA TGTCAGAAAT
    CAACATAAGT CATGTGTTTG TGTTCTAGAT AAGATCCCAG TGTCTGGTTT TAATCAGGAG
    AAGGTAAAGG TGGTCTACTA TAGAGCAATG TATCCCTTTG AGGCCCGCAG TCATGATGAG
    ATCACCATCC ATCCTGGAGA CATAGTCATG GTGGATGAGT CTCAGACAGG TGAGCCTGGA
    TGGTTAGGAG GAGAGATTAA AGGGAAGACT GGCTGGTTTC CTGCCAACTA TGCGGAGAAG
    ATTCCAGAAT CCGAAGTTCC TCTGAGTTTG AGGGCCAGCG CCGCTTCTAG CTCCGCCCCT
    AAATTGGGTT CTCACATGTC ATCTGCTTCT TCATCGACCA CGGCCACGCC CATTCTGCCA
    GTCTCCACAG AGCCTGCATC GATAGCCCCG CCCTCTTCAG CCCCACCCCC CACAGGCCCT
    GCCTCCTCCT CTTCCTCAGC ATCCAGCAAT TGGGCAGATT TCAGCACTAC ATGGCCCTCT
    AATTCTGCTG TGGAGAAGCA GGATAGTGAT GGATGGGATG CATGGCCGAC TCAGCCAACT
    CAGCCTTCTC TGTCAGTGCC CAGTGGAGGG CAGATCAGAC AGCGCTCTGC TTTCACGCCT
    GCTACACTCT CCGGCTCCTC GCCCTCACCC GTGCTTGGAC AGGGTGAGAA GGTGGAGGGT
    CTGCAGGCTC AGGCGCTGTA CCCATGGAGA GCCAAGAAAG ACAATCATCT AAACTTCAAC
    AAGAATGACG TGATCACTGT ACTGGAGCAG CAGGACATGT GGTGGTTTGG GGAGGTGCAA
    GGACAGAGAG GATGGTTCCC TAAATCTTAC GTAAAGCTCA TCTCTGGGCC TGTCCGGAAA
    TCTATGAGTA TTGATTCTGG CTCTTCAGAC AGCCCTCCTA GTGTAAAGAG ACCCAGTCCC
    TCTCTAAACA AACCCACAGA GCTCGGAGAA GGTCAGAACT CCAACAGCAA CTCAATGTAT
    CCGTCTAAAG AGTATGTGGC CATGTATACA TATGAAAGCA ATGAGCAGGG TGACTTGACC
    TTCCAGCAAG GTGATGTCAT CACAGTCACC AAGAAAGAAG GAGATTGGTG GACCGGCACT
    GTGAGTGGGA AGATCGGAGT CTTTCCATCG AATTATGTCA AACCTAAAGA ATCCGAGGGG
    TTGGGATCTG CTGGGAAAAC AGGAAGTTTA GGGAAGAAAC CAGAGATTGC CCAAGTCATC
    GCTCCATACA CGGCCACTGG TGCAGAGCAG CTCACTTTAG CACCAGGCCA GCTCATTCTC
    ATTCGCAAGA AAAACCCGGG AGGATGGTGG GAAGGAGAAC TTCAGGCAAG AGGAAAGAAG
    CGGCAGATTG GCTGGTTTCC TGCTAATTAT GTCAAATTAT TGAGTCCCAG TACCAGTAAG
    ACAACACCCA CAGATCCCAA CCCACCCAAG CTGCCGACAC CCAATGCAGT GTGCCAGGTG
    ATTGGCATGT ACGATTACAC TGCTCAGAAT GATGACGAGC TGCCTTTCGG GAAGGGCCAA
    ATCATCAATG TGTTGAGCAG AGAGGATCCC GATTGGTGGA AAGGAGAGTT GAATGGATCC
    GTCGGCCTCT TCCCCTCGAA CTACGTCAAG CTGACCACCG ACACAGACCC CAGCCAACAA
    TGGTGTGCTG ATCTCCACCT GCTCGACATG CTGACCCCAG TGGAGCGGAA GAGGCAGGGA
    TACATACATG AGCTTATTGT TACAGAGGAG AACTATGTCA ATGACCTGCA GCTGGTCACT
    GAGACCTTCC AGAAGCCTCT GCTGGAGTCT GATTTGTTGA CGGAGAAGGA GGTTGCCATG
    ATCTTTGTCA ACTGGAAAGA GCTGACCATG TGCAACATTA AACTGCTCAA GGCGCTGCGG
    GTGAGGAAGA AGATGTCAGG TGAGCGGATG CCAGTGAAGA TGATTGGTGA CATCCTGACG
    GCTCAGCTTC CTCACATGCA GCCGTACATC CGCTTCTGCA GCTGCCAGCT CAACGGCGCC
    ACCCTCATTC AGCAGAAAAC CGACGAGGTG CCCGAATTCA AGGACTTTGT GAAGAGGTTA
    GCGATGGACC CTCGCTGTAA GGGAATGCCC CTGTCCAGTT TCCTTCTCAA ACCCATGCAG
    AGAGTCACCC GATACCCCCT CATCATCAAA AACATTTTAG AGAACACTCC AGAAAGCCAC
    CCAGACCACA GTCACCTGCG TCTGGCTCTG GAGAAAGCAG AAGAGCTGTG CTCGCAGGTC
    AATGAAGGTG TACGAGAGAA AGAAAACTCC GACCGGCTGG AATGGATACA GGCACATGTG
    CAGTGTGAGG GACTGTCAGA GCAACTTGTG TTCAACTCTG TAACAAACTG TCTGGGCCCT
    CGGAAGTTCC TGCACAGTGG AAAACTCTAC AAAGCCAAGA GCAACAAAGA GCTTTACGGC
    TTCCTTTTTA ACGACTTCCT GCTCCTCACA CAGATAATCA AACCTCTGGG CTCATCCGGG
    ACAGACAAGG TCTTCAGTGC GAAATCCCAT CTGCAGTATC GCATGTACAA AACGCCCATC
    TTTCTCAATG AGGTGCTAGT GAAGCTGCCT ACTGACCCCT CGGGAGATGA ACCCATTTTT
    CACATATCTC ACATCGACAG AGTCTACACT ATACGGGCTG AGAGCATCAA CGAAAGGACA
    GCTTGGGTGC AGAAAATCAA AGCTGCCTCT GAGCTCTTCA TCGAAACCGA AAAGAGGAAG
    AGAGAAAAAG CCTACTTGGT GAGGTCTCAG AGGGCCACTG GCATCGGGCG GCTAATGGTC
    AACATTGTTG AAGGCATCGA ACTGAAGCCC TGCCGCTCAA ATGGTAAGAG TAACCCGTAC
    TGTGAGGTGA CGATGGGCTC TCAGTGTCAC GTCACCAAGA CCCTTCAGGA CACTCTGAAC
    CCCAAGTGGA ACTCAAACTG CCAGTTCTTC ATCAAAGACC TGGAGCAGGA CGTGCTGTGT
    GTCACTGTGT TTGAGAGGGA TCAGTTCTCC CCGGACGATT TCTTGGGCAG GACGGAGATT
    CGGCTGGCCG ACATCAAGAA GGACCAGGGC TCGAAGGGTC CCATCACCAA GCGTCTGCTT
    TTACACGAAG TCCCGACGGG AGAGATTGTG GTGCGACTGG ACCTGCAGCT CTTCGATGAA
    TCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa06106
    Clone ID Related Accession (Same CDS sequence) XM_005170971.4 , XM_005170971.3
    Accession Version XM_005170971.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5256bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product intersectin-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005170971.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCTCAGT TTCCCACACC CTTCGGAGGG GGGTCGGATA CATGGGTAAT ATCTGTGGAC 
    GAGAGAGCCA AACATGACCA GCAGTTTCAC AGTCTCACCC CAACACCCGC TGGCTTCATC
    ACAGGTGACC AGGCAAGGAA TTTCTTCCTG CAGTCAGGTT TACCTGCCCC CATCTTGGCC
    CAGATATGGG CTCTGGCCGA CATGAACAAT GACGGGAAGA TGGACATGCA TGAGTTTTCC
    ATCGCAATGA AGCTGATTAA ATTAAAGCTG CAGGGTCACC CTCTTCCTCC ATCACTGCCC
    CCCTCCATGA AGCAACAGCC CATCTCCATA CCCCCGCCGA CCCCTTTTGG GATACCCTCT
    ATGGGTGCAA TGCCAGGCCT GCCAGCAGTG CCGCCCATGC CTCTGCCCCC TCTGCCTCCA
    GGAGTGCCAG GGATGGGTGT TTCTCCTCCA CTGGGCGCAT CGGTAACCCC ACCCGTACCA
    TCCCTCGCCA ACGGAGCACC CGCAATGATA CAACCCCTGA GTGGATTTTC ACATCCAGGT
    GCCGTGCTCA ACAAAACCCC CTCATTCAAT CGCTCCAGTA TCAAGCTACA GAAGGGCCAG
    TCTGTAGAGG CGCCCAGTGC TCCAGCTGCT CCTCCACCCG CTGACTGGGC CGTGTCTCAG
    TCCTCCAGAC TTAAGTATCG CCAGCTCTTC AACAGCCATG ATAAAATGAT GAGCGGTTAT
    CTCACAGGTC CACAAGCCAG AACTATTCTG ATGCAGTCCA GTCTACCACA AGCCCAGCTG
    GCCACCATCT GGAATCTGTC AGATATAGAT CAGGATGGGA AGTTGACCGC TGAAGAGTTT
    ATTTTGGCCA TGCACTTGAT CGACATGGCC ATGTCTGGTC TCCCTCTTCC CCCTTTGCTG
    CCGCCTGACC TTATACCTCC AACATTCAGG AGAATGCGCT CTGGTAGTGG AGTGTCTGTG
    ACTAGCATGC ACTCGACAGA CTTGCGGTCT CAGGAAGAGC CAGAGGAGGA GGAGAAAGAG
    GCCGAGAAAA AACTTCCAGT CACATTTGAG GATAAGAAAC GAGAGAACTT TGAGCGCGGA
    AACCTGGAGT TGGAGAAACG TCGACAGGCA CTTCTGGAGC AGCAGAGGAA AGAGCAGGAG
    CGACTGGCGG CGCTGGAGAA AGAAGAGCAG GAGCGGAAGG AGAGGGAACG ACTGGAGCAG
    GAGAGACGCA GACAGCAGGA GCTGGACAAA CAGCTGGAGA GACAGAGGGA GCTGGAGCGA
    CAGCGGGAGG AGGAGAGACG CAAGGAGATC GAGAGAAGAG AGGCTGCTAA ACGGGAGCTG
    GAGCGACAGA GGCAGCTGGA GTGGGAGAGG ACGCGCAGAC AGGAGCTGCT CACCCAAAGG
    AACAGAGAAC AGGAGAACAT CGTCCTGCTC AAAGCACGGA AGAAAACTCT CGAGTTTGAG
    CTGGAGGCTC TGAATGATAA GAAGTCTCAG TTGGAGGGGA AACTGCAGGA TATTCGGTCC
    CGTCTCTCGA TTCAGAGGCA TGAGATCGAG ACCACCAATA AGACTCGGGA GCTCCGCATT
    GCAGAGATCA CAAGCCTGCA ACAGCAGCTG CAGGAGTCTC AGCAGTGGTT GAGTCGCTTG
    ATTCCTGATA AACAGTGTCT GAATGACCAG CTGAAGCAGG TTCAGCAGAA CAGTCTGCAC
    AGAGACTCTT TGAACAGTCT TCAGAGGGCC ATTGAAATGA AAGAATCCAC CAAACAGCAG
    CTGAAAGAAC AGCTGGATGC TGTGGAGAAG GAAACTCGCT CCAAACTATT GGAGATCGAC
    GCCTTCAACA CACAGCTGAA GGAGCTGAGA GAAATCCACA ACAAACAGCA GAGGCAGAAA
    CAGAAGGAGC TGGAGGCTGA CATCACCCAG CCGTCACATG ACAGGAAGTC CATAGACTCA
    CAAGACTCGA GGTTGTCGGG GACTGATGAT GGCGTGTCTC CAGCGTGGAG GGATGATGGG
    CTGGGTAAAG CTCCTACTCC TCCTGTGTCT CAGGCCTGGA TGAGCCGGGT GAGTGAAGAA
    GAAAACCACA GCCGAGCTGA AATACAGGAT AACCTCTCCA AGCTTTTCAC CCAACATCCA
    CAAGCAGGAA AAATACAGGC TCAGTCTCCC TGGTCCATGG CAGATAAGAT CCCAGTGTCT
    GGTTTTAATC AGGAGAAGGT AAAGGTGGTC TACTATAGAG CAATGTATCC CTTTGAGGCC
    CGCAGTCATG ATGAGATCAC CATCCATCCT GGAGACATAG TCATGGTGAA GGGAGAGTGG
    GTGGATGAGT CTCAGACAGG TGAGCCTGGA TGGTTAGGAG GAGAGATTAA AGGGAAGACT
    GGCTGGTTTC CTGCCAACTA TGCGGAGAAG ATTCCAGAAT CCGAAGTTCC TCTGAGTTTG
    AGGGCCAGCG CTGCTTCTAG CTCCGCCCCT AAATTGGGTT CTCACATGTC ATCTGCTTCT
    TCATCGACCA CGGCCACGCC CATTCTGCCA GTCTCCACAG AGCCTGCATC GATAGCCCCG
    CCCTCTTCAG CCCCACCCCC CACAGGCCCC GCCTCCTCCT CTTCCTCAGC ATCAAGCAAT
    TGGGCAGATT TCAGCACTAC ATGGCCCTCT AATTCTGCTG TGGAGAAGCA GGATAGTGAT
    GGATGGGATG CATGGCCGAC TCAGCCAACT CAGCCTTCTC TGTCAGTGCC CAGTGGAGGG
    CAGATCAGAC AGCGCTCTGC TTTCACGCCT GCTACACTCT CCGGCTCCTC CCCCTCACCC
    GTGCTTGGAC AGGGTGAGAA GGTGGAGGGT CTGCAGGCTC AGGCGCTGTA CCCATGGAGA
    GCCAAGAAAG ACAATCATCT AAACTTCAAC AAGAATGACG TGATCACTGT ACTGGAGCAG
    CAGGACATGT GGTGGTTTGG GGAGGTGCAA GGACAGAGAG GATGGTTCCC TAAATCTTAC
    GTCAAGCTCA TCTCTGGACC TGTCCGGAAA TCTATGAGTA TTGAGTCTGG CTCTTCAGAC
    AGCCCTCCTA GTGTAAAGAG ACCCAGTCCC TCTCTAAACA AACCCACAGA GCTCGGAGAA
    GGTCAGAACT CCAACAGCAA CTCAATGTAT CCGTCTAAAG AGTATGTGGC CATGTATACA
    TATGAAAGCA ATGAGCAGGG TGACTTGACC TTCCAGCAAG GTGATGTCAT CACAGTCACC
    AAGAAAGAAG GAGATTGGTG GACCGGCACT GTGAGCGGGA AGATCGGAGT CTTTCCATCG
    AATTATGTCA AACCTAAAGA ATCTGAGGGG TTGGGATCTG CTGGGAAAAC AGGAAGTTTA
    GGGAAGAAAC CAGAGATTGC CCAAGTCATC GCTCCATACA CGGCCACTGG TGCAGAGCAG
    CTCACTTTAG CACCAGGCCA GCTCATTCTC ATTCGCAAGA AAAACCCGGG AGGATGGTGG
    GAAGGAGAAC TTCAGGCAAG AGGAAAGAAG CGGCAGATTG GCTGGTTTCC TGCAAATTAT
    GTCAAATTAT TGAGTCCCAG TACCAGTAAG ACAACACCCA CAGATCCCAA CCCACCCAAG
    CTGCCGACAC CCAATGCAGT GTGCCAGGTG ATTGGCATGT ATGATTACAC TGCTCAGAAT
    GATGACGAGC TGCCTTTCGG GAAGGGCCAA ATCATCAATG TGTTGAGCAG AGAGGATCCC
    GATTGGTGGA AAGGAGAGTT GAATGGATCC GTCGGCCTCT TCCCCTCGAA CTACGTCAAG
    CTGACCACCG ACACAGACCC CAGCCAACAA TGGTGTGCTG ATCTCCACCT GCTCGACATG
    CTGACCCCAG TGGAGCGGAA GAGGCAGGGA TACATACATG AGCTTATTGT TACAGAGGAG
    AACTATGTCA ATGACCTGCA GCTGGTCACT GAGACCTTCC AGAAGCCTCT GCTGGAGTCT
    GATTTGTTGA CGGAGAAGGA GGTTGCCATG ATCTTTGTCA ACTGGAAAGA GCTGACCATG
    TGCAACATTA AACTGCTCAA GGCGCTGCGG GTGAGGAAGA AGATGTCAGG TGAGCGGATG
    CCAGTGAAGA TGATTGGTGA CATCCTGACG GCTCAGCTTC CTCACATGCA GCCATACATC
    CGCTTCTGCA GCTGCCAGCT CAACGGCGCC ACCCTCATTC AGCAGAAAAC TGACGAGGTG
    CCCGAATTCA AGGACTTTGT GAAGAGGTTA GCGATGGACC CTCGCTGTAA GGGAATGCCC
    CTGTCCAGTT TCCTTCTCAA ACCCATGCAG AGAGTCACCC GATACCCCCT CATCATCAAA
    AACATTTTAG AGAACACTCC AGAAAGCCAC CCGGACCACA GTCACCTGCG TCTGGCTCTG
    GAGAAAGCAG AAGAGCTGTG CTCGCAGGTC AATGAAGGTG TACGAGAGAA AGAAAACTCC
    GACCGGCTGG AATGGATACA GGCACATGTG CAGTGTGAGG GACTGTCAGA GCAACTTGTG
    TTCAACTCTG TAACAAACTG TCTGGGCCCT CGGAAGTTCC TGCACAGTGG AAAACTCTAC
    AAAGCCAAGA GCAACAAAGA GCTTTACGGC TTCCTTTTTA ACGACTTCCT GCTCCTCACA
    CAGATAATCA AACCTCTGGG CTCATCCGGG ACAGACAAGG TCTTCAGTGC GAAATCCCAT
    CTGCAGTATC GCATGTACAA AACGCCCATC TTTCTCAATG AGGTGCTAGT GAAGCTGCCT
    ACTGACCCCT CGGGAGATGA ACCCATTTTT CACATATCTC ACATTGACAG AGTCTACACT
    ATACGGGCTG AGAGCATCAA CGAAAGGACA GCTTGGGTGC AGAAAATCAA AGCTGCCTCT
    GAGCTCTTCA TCGAAACCGA AAAGAGGAAG AGAGAGAAAG CCTACTTGGT GAGGTCTCAG
    AGGGCCACTG GCATCGGGCG GCTAATGGTC AACATTGTTG AAGGCATCGA ACTGAAGCCC
    TGCCGCTCAA ATGGTAAGAG TAACCCGTAC TGTGAGGTGA CGATGGGCTC TCAGTGTCAC
    GTCACCAAGA CCCTTCAGGA CACTCTGAAC CCCAAGTGGA ACTCAAACTG CCAGTTCTTC
    ATCAAAGACC TGGAGCAGGA CGTGCTGTGT GTCACTGTGT TTGAGAGGGA TCAGTTCTCC
    CCGGACGATT TCTTGGGCAG GACGGAGATT CGGCTGGCCG ACATCAAGAA GGACCAGGGC
    TCGAAGGGTC CCATCACCAA GCGTCTGCTT TTACACGAAG TCCCGACGGG AGAGATTGTG
    GTGCGACTGG ACCTGCAGCT CTTCGATGAA CCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005171028.1
    CDS227..5482
    Translation

    Target ORF information:

    RefSeq Version XM_005170971.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio intersectin 1 (SH3 domain protein) (itsn1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005170971.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCTCAGT TTCCCACACC CTTCGGAGGG GGGTCGGATA CATGGGTAAT ATCTGTGGAC 
    GAGAGAGCCA AACATGACCA GCAGTTTCAC AGTCTCACCC CAACACCCGC TGGCTTCATC
    ACAGGTGACC AGGCAAGGAA TTTCTTCCTG CAGTCAGGTT TACCTGCCCC CATCTTGGCC
    CAGATATGGG CTCTGGCCGA CATGAACAAT GACGGGAAGA TGGACATGCA TGAGTTTTCC
    ATCGCAATGA AGCTGATTAA ATTAAAGCTG CAGGGTCACC CTCTTCCTCC ATCACTGCCC
    CCCTCCATGA AGCAACAGCC CATCTCCATA CCCCCGCCGA CCCCTTTTGG GATACCCTCT
    ATGGGTGCAA TGCCAGGCCT GCCAGCAGTG CCGCCCATGC CTCTGCCCCC TCTGCCTCCA
    GGAGTGCCAG GGATGGGTGT TTCTCCTCCA CTGGGCGCAT CGGTAACCCC ACCCGTACCA
    TCCCTCGCCA ACGGAGCACC CGCAATGATA CAACCCCTGA GTGGATTTTC ACATCCAGGT
    GCCGTGCTCA ACAAAACCCC CTCATTCAAT CGCTCCAGTA TCAAGCTACA GAAGGGCCAG
    TCTGTAGAGG CGCCCAGTGC TCCAGCTGCT CCTCCACCCG CTGACTGGGC CGTGTCTCAG
    TCCTCCAGAC TTAAGTATCG CCAGCTCTTC AACAGCCATG ATAAAATGAT GAGCGGTTAT
    CTCACAGGTC CACAAGCCAG AACTATTCTG ATGCAGTCCA GTCTACCACA AGCCCAGCTG
    GCCACCATCT GGAATCTGTC AGATATAGAT CAGGATGGGA AGTTGACCGC TGAAGAGTTT
    ATTTTGGCCA TGCACTTGAT CGACATGGCC ATGTCTGGTC TCCCTCTTCC CCCTTTGCTG
    CCGCCTGACC TTATACCTCC AACATTCAGG AGAATGCGCT CTGGTAGTGG AGTGTCTGTG
    ACTAGCATGC ACTCGACAGA CTTGCGGTCT CAGGAAGAGC CAGAGGAGGA GGAGAAAGAG
    GCCGAGAAAA AACTTCCAGT CACATTTGAG GATAAGAAAC GAGAGAACTT TGAGCGCGGA
    AACCTGGAGT TGGAGAAACG TCGACAGGCA CTTCTGGAGC AGCAGAGGAA AGAGCAGGAG
    CGACTGGCGG CGCTGGAGAA AGAAGAGCAG GAGCGGAAGG AGAGGGAACG ACTGGAGCAG
    GAGAGACGCA GACAGCAGGA GCTGGACAAA CAGCTGGAGA GACAGAGGGA GCTGGAGCGA
    CAGCGGGAGG AGGAGAGACG CAAGGAGATC GAGAGAAGAG AGGCTGCTAA ACGGGAGCTG
    GAGCGACAGA GGCAGCTGGA GTGGGAGAGG ACGCGCAGAC AGGAGCTGCT CACCCAAAGG
    AACAGAGAAC AGGAGAACAT CGTCCTGCTC AAAGCACGGA AGAAAACTCT CGAGTTTGAG
    CTGGAGGCTC TGAATGATAA GAAGTCTCAG TTGGAGGGGA AACTGCAGGA TATTCGGTCC
    CGTCTCTCGA TTCAGAGGCA TGAGATCGAG ACCACCAATA AGACTCGGGA GCTCCGCATT
    GCAGAGATCA CAAGCCTGCA ACAGCAGCTG CAGGAGTCTC AGCAGTGGTT GAGTCGCTTG
    ATTCCTGATA AACAGTGTCT GAATGACCAG CTGAAGCAGG TTCAGCAGAA CAGTCTGCAC
    AGAGACTCTT TGAACAGTCT TCAGAGGGCC ATTGAAATGA AAGAATCCAC CAAACAGCAG
    CTGAAAGAAC AGCTGGATGC TGTGGAGAAG GAAACTCGCT CCAAACTATT GGAGATCGAC
    GCCTTCAACA CACAGCTGAA GGAGCTGAGA GAAATCCACA ACAAACAGCA GAGGCAGAAA
    CAGAAGGAGC TGGAGGCTGA CATCACCCAG CCGTCACATG ACAGGAAGTC CATAGACTCA
    CAAGACTCGA GGTTGTCGGG GACTGATGAT GGCGTGTCTC CAGCGTGGAG GGATGATGGG
    CTGGGTAAAG CTCCTACTCC TCCTGTGTCT CAGGCCTGGA TGAGCCGGGT GAGTGAAGAA
    GAAAACCACA GCCGAGCTGA AATACAGGAT AACCTCTCCA AGCTTTTCAC CCAACATCCA
    CAAGCAGGAA AAATACAGGC TCAGTCTCCC TGGTCCATGG CAGATAAGAT CCCAGTGTCT
    GGTTTTAATC AGGAGAAGGT AAAGGTGGTC TACTATAGAG CAATGTATCC CTTTGAGGCC
    CGCAGTCATG ATGAGATCAC CATCCATCCT GGAGACATAG TCATGGTGAA GGGAGAGTGG
    GTGGATGAGT CTCAGACAGG TGAGCCTGGA TGGTTAGGAG GAGAGATTAA AGGGAAGACT
    GGCTGGTTTC CTGCCAACTA TGCGGAGAAG ATTCCAGAAT CCGAAGTTCC TCTGAGTTTG
    AGGGCCAGCG CTGCTTCTAG CTCCGCCCCT AAATTGGGTT CTCACATGTC ATCTGCTTCT
    TCATCGACCA CGGCCACGCC CATTCTGCCA GTCTCCACAG AGCCTGCATC GATAGCCCCG
    CCCTCTTCAG CCCCACCCCC CACAGGCCCC GCCTCCTCCT CTTCCTCAGC ATCAAGCAAT
    TGGGCAGATT TCAGCACTAC ATGGCCCTCT AATTCTGCTG TGGAGAAGCA GGATAGTGAT
    GGATGGGATG CATGGCCGAC TCAGCCAACT CAGCCTTCTC TGTCAGTGCC CAGTGGAGGG
    CAGATCAGAC AGCGCTCTGC TTTCACGCCT GCTACACTCT CCGGCTCCTC CCCCTCACCC
    GTGCTTGGAC AGGGTGAGAA GGTGGAGGGT CTGCAGGCTC AGGCGCTGTA CCCATGGAGA
    GCCAAGAAAG ACAATCATCT AAACTTCAAC AAGAATGACG TGATCACTGT ACTGGAGCAG
    CAGGACATGT GGTGGTTTGG GGAGGTGCAA GGACAGAGAG GATGGTTCCC TAAATCTTAC
    GTCAAGCTCA TCTCTGGACC TGTCCGGAAA TCTATGAGTA TTGAGTCTGG CTCTTCAGAC
    AGCCCTCCTA GTGTAAAGAG ACCCAGTCCC TCTCTAAACA AACCCACAGA GCTCGGAGAA
    GGTCAGAACT CCAACAGCAA CTCAATGTAT CCGTCTAAAG AGTATGTGGC CATGTATACA
    TATGAAAGCA ATGAGCAGGG TGACTTGACC TTCCAGCAAG GTGATGTCAT CACAGTCACC
    AAGAAAGAAG GAGATTGGTG GACCGGCACT GTGAGCGGGA AGATCGGAGT CTTTCCATCG
    AATTATGTCA AACCTAAAGA ATCTGAGGGG TTGGGATCTG CTGGGAAAAC AGGAAGTTTA
    GGGAAGAAAC CAGAGATTGC CCAAGTCATC GCTCCATACA CGGCCACTGG TGCAGAGCAG
    CTCACTTTAG CACCAGGCCA GCTCATTCTC ATTCGCAAGA AAAACCCGGG AGGATGGTGG
    GAAGGAGAAC TTCAGGCAAG AGGAAAGAAG CGGCAGATTG GCTGGTTTCC TGCAAATTAT
    GTCAAATTAT TGAGTCCCAG TACCAGTAAG ACAACACCCA CAGATCCCAA CCCACCCAAG
    CTGCCGACAC CCAATGCAGT GTGCCAGGTG ATTGGCATGT ATGATTACAC TGCTCAGAAT
    GATGACGAGC TGCCTTTCGG GAAGGGCCAA ATCATCAATG TGTTGAGCAG AGAGGATCCC
    GATTGGTGGA AAGGAGAGTT GAATGGATCC GTCGGCCTCT TCCCCTCGAA CTACGTCAAG
    CTGACCACCG ACACAGACCC CAGCCAACAA TGGTGTGCTG ATCTCCACCT GCTCGACATG
    CTGACCCCAG TGGAGCGGAA GAGGCAGGGA TACATACATG AGCTTATTGT TACAGAGGAG
    AACTATGTCA ATGACCTGCA GCTGGTCACT GAGACCTTCC AGAAGCCTCT GCTGGAGTCT
    GATTTGTTGA CGGAGAAGGA GGTTGCCATG ATCTTTGTCA ACTGGAAAGA GCTGACCATG
    TGCAACATTA AACTGCTCAA GGCGCTGCGG GTGAGGAAGA AGATGTCAGG TGAGCGGATG
    CCAGTGAAGA TGATTGGTGA CATCCTGACG GCTCAGCTTC CTCACATGCA GCCATACATC
    CGCTTCTGCA GCTGCCAGCT CAACGGCGCC ACCCTCATTC AGCAGAAAAC TGACGAGGTG
    CCCGAATTCA AGGACTTTGT GAAGAGGTTA GCGATGGACC CTCGCTGTAA GGGAATGCCC
    CTGTCCAGTT TCCTTCTCAA ACCCATGCAG AGAGTCACCC GATACCCCCT CATCATCAAA
    AACATTTTAG AGAACACTCC AGAAAGCCAC CCGGACCACA GTCACCTGCG TCTGGCTCTG
    GAGAAAGCAG AAGAGCTGTG CTCGCAGGTC AATGAAGGTG TACGAGAGAA AGAAAACTCC
    GACCGGCTGG AATGGATACA GGCACATGTG CAGTGTGAGG GACTGTCAGA GCAACTTGTG
    TTCAACTCTG TAACAAACTG TCTGGGCCCT CGGAAGTTCC TGCACAGTGG AAAACTCTAC
    AAAGCCAAGA GCAACAAAGA GCTTTACGGC TTCCTTTTTA ACGACTTCCT GCTCCTCACA
    CAGATAATCA AACCTCTGGG CTCATCCGGG ACAGACAAGG TCTTCAGTGC GAAATCCCAT
    CTGCAGTATC GCATGTACAA AACGCCCATC TTTCTCAATG AGGTGCTAGT GAAGCTGCCT
    ACTGACCCCT CGGGAGATGA ACCCATTTTT CACATATCTC ACATTGACAG AGTCTACACT
    ATACGGGCTG AGAGCATCAA CGAAAGGACA GCTTGGGTGC AGAAAATCAA AGCTGCCTCT
    GAGCTCTTCA TCGAAACCGA AAAGAGGAAG AGAGAGAAAG CCTACTTGGT GAGGTCTCAG
    AGGGCCACTG GCATCGGGCG GCTAATGGTC AACATTGTTG AAGGCATCGA ACTGAAGCCC
    TGCCGCTCAA ATGGTAAGAG TAACCCGTAC TGTGAGGTGA CGATGGGCTC TCAGTGTCAC
    GTCACCAAGA CCCTTCAGGA CACTCTGAAC CCCAAGTGGA ACTCAAACTG CCAGTTCTTC
    ATCAAAGACC TGGAGCAGGA CGTGCTGTGT GTCACTGTGT TTGAGAGGGA TCAGTTCTCC
    CCGGACGATT TCTTGGGCAG GACGGAGATT CGGCTGGCCG ACATCAAGAA GGACCAGGGC
    TCGAAGGGTC CCATCACCAA GCGTCTGCTT TTACACGAAG TCCCGACGGG AGAGATTGTG
    GTGCGACTGG ACCTGCAGCT CTTCGATGAA CCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa06106
    Clone ID Related Accession (Same CDS sequence) XM_005170971.4 , XM_005170971.3
    Accession Version XM_005170971.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5256bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product intersectin-1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005170971.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCTCAGT TTCCCACACC CTTCGGAGGG GGGTCGGATA CATGGGTAAT ATCTGTGGAC 
    GAGAGAGCCA AACATGACCA GCAGTTTCAC AGTCTCACCC CAACACCCGC TGGCTTCATC
    ACAGGTGACC AGGCAAGGAA TTTCTTCCTG CAGTCAGGTT TACCTGCCCC CATCTTGGCC
    CAGATATGGG CTCTGGCCGA CATGAACAAT GACGGGAAGA TGGACATGCA TGAGTTTTCC
    ATCGCAATGA AGCTGATTAA ATTAAAGCTG CAGGGTCACC CTCTTCCTCC ATCACTGCCC
    CCCTCCATGA AGCAACAGCC CATCTCCATA CCCCCGCCGA CCCCTTTTGG GATACCCTCT
    ATGGGTGCAA TGCCAGGCCT GCCAGCAGTG CCGCCCATGC CTCTGCCCCC TCTGCCTCCA
    GGAGTGCCAG GGATGGGTGT TTCTCCTCCA CTGGGCGCAT CGGTAACCCC ACCCGTACCA
    TCCCTCGCCA ACGGAGCACC CGCAATGATA CAACCCCTGA GTGGATTTTC ACATCCAGGT
    GCCGTGCTCA ACAAAACCCC CTCATTCAAT CGCTCCAGTA TCAAGCTACA GAAGGGCCAG
    TCTGTAGAGG CGCCCAGTGC TCCAGCTGCT CCTCCACCCG CTGACTGGGC CGTGTCTCAG
    TCCTCCAGAC TTAAGTATCG CCAGCTCTTC AACAGCCATG ATAAAATGAT GAGCGGTTAT
    CTCACAGGTC CACAAGCCAG AACTATTCTG ATGCAGTCCA GTCTACCACA AGCCCAGCTG
    GCCACCATCT GGAATCTGTC AGATATAGAT CAGGATGGGA AGTTGACCGC TGAAGAGTTT
    ATTTTGGCCA TGCACTTGAT CGACATGGCC ATGTCTGGTC TCCCTCTTCC CCCTTTGCTG
    CCGCCTGACC TTATACCTCC AACATTCAGG AGAATGCGCT CTGGTAGTGG AGTGTCTGTG
    ACTAGCATGC ACTCGACAGA CTTGCGGTCT CAGGAAGAGC CAGAGGAGGA GGAGAAAGAG
    GCCGAGAAAA AACTTCCAGT CACATTTGAG GATAAGAAAC GAGAGAACTT TGAGCGCGGA
    AACCTGGAGT TGGAGAAACG TCGACAGGCA CTTCTGGAGC AGCAGAGGAA AGAGCAGGAG
    CGACTGGCGG CGCTGGAGAA AGAAGAGCAG GAGCGGAAGG AGAGGGAACG ACTGGAGCAG
    GAGAGACGCA GACAGCAGGA GCTGGACAAA CAGCTGGAGA GACAGAGGGA GCTGGAGCGA
    CAGCGGGAGG AGGAGAGACG CAAGGAGATC GAGAGAAGAG AGGCTGCTAA ACGGGAGCTG
    GAGCGACAGA GGCAGCTGGA GTGGGAGAGG ACGCGCAGAC AGGAGCTGCT CACCCAAAGG
    AACAGAGAAC AGGAGAACAT CGTCCTGCTC AAAGCACGGA AGAAAACTCT CGAGTTTGAG
    CTGGAGGCTC TGAATGATAA GAAGTCTCAG TTGGAGGGGA AACTGCAGGA TATTCGGTCC
    CGTCTCTCGA TTCAGAGGCA TGAGATCGAG ACCACCAATA AGACTCGGGA GCTCCGCATT
    GCAGAGATCA CAAGCCTGCA ACAGCAGCTG CAGGAGTCTC AGCAGTGGTT GAGTCGCTTG
    ATTCCTGATA AACAGTGTCT GAATGACCAG CTGAAGCAGG TTCAGCAGAA CAGTCTGCAC
    AGAGACTCTT TGAACAGTCT TCAGAGGGCC ATTGAAATGA AAGAATCCAC CAAACAGCAG
    CTGAAAGAAC AGCTGGATGC TGTGGAGAAG GAAACTCGCT CCAAACTATT GGAGATCGAC
    GCCTTCAACA CACAGCTGAA GGAGCTGAGA GAAATCCACA ACAAACAGCA GAGGCAGAAA
    CAGAAGGAGC TGGAGGCTGA CATCACCCAG CCGTCACATG ACAGGAAGTC CATAGACTCA
    CAAGACTCGA GGTTGTCGGG GACTGATGAT GGCGTGTCTC CAGCGTGGAG GGATGATGGG
    CTGGGTAAAG CTCCTACTCC TCCTGTGTCT CAGGCCTGGA TGAGCCGGGT GAGTGAAGAA
    GAAAACCACA GCCGAGCTGA AATACAGGAT AACCTCTCCA AGCTTTTCAC CCAACATCCA
    CAAGCAGGAA AAATACAGGC TCAGTCTCCC TGGTCCATGG CAGATAAGAT CCCAGTGTCT
    GGTTTTAATC AGGAGAAGGT AAAGGTGGTC TACTATAGAG CAATGTATCC CTTTGAGGCC
    CGCAGTCATG ATGAGATCAC CATCCATCCT GGAGACATAG TCATGGTGAA GGGAGAGTGG
    GTGGATGAGT CTCAGACAGG TGAGCCTGGA TGGTTAGGAG GAGAGATTAA AGGGAAGACT
    GGCTGGTTTC CTGCCAACTA TGCGGAGAAG ATTCCAGAAT CCGAAGTTCC TCTGAGTTTG
    AGGGCCAGCG CTGCTTCTAG CTCCGCCCCT AAATTGGGTT CTCACATGTC ATCTGCTTCT
    TCATCGACCA CGGCCACGCC CATTCTGCCA GTCTCCACAG AGCCTGCATC GATAGCCCCG
    CCCTCTTCAG CCCCACCCCC CACAGGCCCC GCCTCCTCCT CTTCCTCAGC ATCAAGCAAT
    TGGGCAGATT TCAGCACTAC ATGGCCCTCT AATTCTGCTG TGGAGAAGCA GGATAGTGAT
    GGATGGGATG CATGGCCGAC TCAGCCAACT CAGCCTTCTC TGTCAGTGCC CAGTGGAGGG
    CAGATCAGAC AGCGCTCTGC TTTCACGCCT GCTACACTCT CCGGCTCCTC CCCCTCACCC
    GTGCTTGGAC AGGGTGAGAA GGTGGAGGGT CTGCAGGCTC AGGCGCTGTA CCCATGGAGA
    GCCAAGAAAG ACAATCATCT AAACTTCAAC AAGAATGACG TGATCACTGT ACTGGAGCAG
    CAGGACATGT GGTGGTTTGG GGAGGTGCAA GGACAGAGAG GATGGTTCCC TAAATCTTAC
    GTCAAGCTCA TCTCTGGACC TGTCCGGAAA TCTATGAGTA TTGAGTCTGG CTCTTCAGAC
    AGCCCTCCTA GTGTAAAGAG ACCCAGTCCC TCTCTAAACA AACCCACAGA GCTCGGAGAA
    GGTCAGAACT CCAACAGCAA CTCAATGTAT CCGTCTAAAG AGTATGTGGC CATGTATACA
    TATGAAAGCA ATGAGCAGGG TGACTTGACC TTCCAGCAAG GTGATGTCAT CACAGTCACC
    AAGAAAGAAG GAGATTGGTG GACCGGCACT GTGAGCGGGA AGATCGGAGT CTTTCCATCG
    AATTATGTCA AACCTAAAGA ATCTGAGGGG TTGGGATCTG CTGGGAAAAC AGGAAGTTTA
    GGGAAGAAAC CAGAGATTGC CCAAGTCATC GCTCCATACA CGGCCACTGG TGCAGAGCAG
    CTCACTTTAG CACCAGGCCA GCTCATTCTC ATTCGCAAGA AAAACCCGGG AGGATGGTGG
    GAAGGAGAAC TTCAGGCAAG AGGAAAGAAG CGGCAGATTG GCTGGTTTCC TGCAAATTAT
    GTCAAATTAT TGAGTCCCAG TACCAGTAAG ACAACACCCA CAGATCCCAA CCCACCCAAG
    CTGCCGACAC CCAATGCAGT GTGCCAGGTG ATTGGCATGT ATGATTACAC TGCTCAGAAT
    GATGACGAGC TGCCTTTCGG GAAGGGCCAA ATCATCAATG TGTTGAGCAG AGAGGATCCC
    GATTGGTGGA AAGGAGAGTT GAATGGATCC GTCGGCCTCT TCCCCTCGAA CTACGTCAAG
    CTGACCACCG ACACAGACCC CAGCCAACAA TGGTGTGCTG ATCTCCACCT GCTCGACATG
    CTGACCCCAG TGGAGCGGAA GAGGCAGGGA TACATACATG AGCTTATTGT TACAGAGGAG
    AACTATGTCA ATGACCTGCA GCTGGTCACT GAGACCTTCC AGAAGCCTCT GCTGGAGTCT
    GATTTGTTGA CGGAGAAGGA GGTTGCCATG ATCTTTGTCA ACTGGAAAGA GCTGACCATG
    TGCAACATTA AACTGCTCAA GGCGCTGCGG GTGAGGAAGA AGATGTCAGG TGAGCGGATG
    CCAGTGAAGA TGATTGGTGA CATCCTGACG GCTCAGCTTC CTCACATGCA GCCATACATC
    CGCTTCTGCA GCTGCCAGCT CAACGGCGCC ACCCTCATTC AGCAGAAAAC TGACGAGGTG
    CCCGAATTCA AGGACTTTGT GAAGAGGTTA GCGATGGACC CTCGCTGTAA GGGAATGCCC
    CTGTCCAGTT TCCTTCTCAA ACCCATGCAG AGAGTCACCC GATACCCCCT CATCATCAAA
    AACATTTTAG AGAACACTCC AGAAAGCCAC CCGGACCACA GTCACCTGCG TCTGGCTCTG
    GAGAAAGCAG AAGAGCTGTG CTCGCAGGTC AATGAAGGTG TACGAGAGAA AGAAAACTCC
    GACCGGCTGG AATGGATACA GGCACATGTG CAGTGTGAGG GACTGTCAGA GCAACTTGTG
    TTCAACTCTG TAACAAACTG TCTGGGCCCT CGGAAGTTCC TGCACAGTGG AAAACTCTAC
    AAAGCCAAGA GCAACAAAGA GCTTTACGGC TTCCTTTTTA ACGACTTCCT GCTCCTCACA
    CAGATAATCA AACCTCTGGG CTCATCCGGG ACAGACAAGG TCTTCAGTGC GAAATCCCAT
    CTGCAGTATC GCATGTACAA AACGCCCATC TTTCTCAATG AGGTGCTAGT GAAGCTGCCT
    ACTGACCCCT CGGGAGATGA ACCCATTTTT CACATATCTC ACATTGACAG AGTCTACACT
    ATACGGGCTG AGAGCATCAA CGAAAGGACA GCTTGGGTGC AGAAAATCAA AGCTGCCTCT
    GAGCTCTTCA TCGAAACCGA AAAGAGGAAG AGAGAGAAAG CCTACTTGGT GAGGTCTCAG
    AGGGCCACTG GCATCGGGCG GCTAATGGTC AACATTGTTG AAGGCATCGA ACTGAAGCCC
    TGCCGCTCAA ATGGTAAGAG TAACCCGTAC TGTGAGGTGA CGATGGGCTC TCAGTGTCAC
    GTCACCAAGA CCCTTCAGGA CACTCTGAAC CCCAAGTGGA ACTCAAACTG CCAGTTCTTC
    ATCAAAGACC TGGAGCAGGA CGTGCTGTGT GTCACTGTGT TTGAGAGGGA TCAGTTCTCC
    CCGGACGATT TCTTGGGCAG GACGGAGATT CGGCTGGCCG ACATCAAGAA GGACCAGGGC
    TCGAAGGGTC CCATCACCAA GCGTCTGCTT TTACACGAAG TCCCGACGGG AGAGATTGTG
    GTGCGACTGG ACCTGCAGCT CTTCGATGAA CCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005171028.1
    CDS123..5378
    Translation

    Target ORF information:

    RefSeq Version XM_005170971.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio intersectin 1 (SH3 domain protein) (itsn1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005170971.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCTCAGT TTCCCACACC CTTCGGAGGG GGGTCGGATA CATGGGTAAT ATCTGTGGAC 
    GAGAGAGCCA AACATGACCA GCAGTTTCAC AGTCTCACCC CAACACCCGC TGGCTTCATC
    ACAGGTGACC AGGCAAGGAA TTTCTTCCTG CAGTCAGGTT TACCTGCCCC CATCTTGGCC
    CAGATATGGG CTCTGGCCGA CATGAACAAT GACGGGAAGA TGGACATGCA TGAGTTTTCC
    ATCGCAATGA AGCTGATTAA ATTAAAGCTG CAGGGTCACC CTCTTCCTCC ATCACTGCCC
    CCCTCCATGA AGCAACAGCC CATCTCCATA CCCCCGCCGA CCCCTTTTGG GATACCCTCT
    ATGGGTGCAA TGCCAGGCCT GCCAGCAGTG CCGCCCATGC CTCTGCCCCC TCTGCCTCCA
    GGAGTGCCAG GGATGGGTGT TTCTCCTCCA CTGGGCGCAT CGGTAACCCC ACCCGTACCA
    TCCCTCGCCA ACGGAGCACC CGCAATGATA CAACCCCTGA GTGGATTTTC ACATCCAGGT
    GCCGTGCTCA ACAAAACCCC CTCATTCAAT CGCTCCAGTA TCAAGCTACA GAAGGGCCAG
    TCTGTAGAGG CGCCCAGTGC TCCAGCTGCT CCTCCACCCG CTGACTGGGC CGTGTCTCAG
    TCCTCCAGAC TTAAGTATCG CCAGCTCTTC AACAGCCATG ATAAAATGAT GAGCGGTTAT
    CTCACAGGTC CACAAGCCAG AACTATTCTG ATGCAGTCCA GTCTACCACA AGCCCAGCTG
    GCCACCATCT GGAATCTGTC AGATATAGAT CAGGATGGGA AGTTGACCGC TGAAGAGTTT
    ATTTTGGCCA TGCACTTGAT CGACATGGCC ATGTCTGGTC TCCCTCTTCC CCCTTTGCTG
    CCGCCTGACC TTATACCTCC AACATTCAGG AGAATGCGCT CTGGTAGTGG AGTGTCTGTG
    ACTAGCATGC ACTCGACAGA CTTGCGGTCT CAGGAAGAGC CAGAGGAGGA GGAGAAAGAG
    GCCGAGAAAA AACTTCCAGT CACATTTGAG GATAAGAAAC GAGAGAACTT TGAGCGCGGA
    AACCTGGAGT TGGAGAAACG TCGACAGGCA CTTCTGGAGC AGCAGAGGAA AGAGCAGGAG
    CGACTGGCGG CGCTGGAGAA AGAAGAGCAG GAGCGGAAGG AGAGGGAACG ACTGGAGCAG
    GAGAGACGCA GACAGCAGGA GCTGGACAAA CAGCTGGAGA GACAGAGGGA GCTGGAGCGA
    CAGCGGGAGG AGGAGAGACG CAAGGAGATC GAGAGAAGAG AGGCTGCTAA ACGGGAGCTG
    GAGCGACAGA GGCAGCTGGA GTGGGAGAGG ACGCGCAGAC AGGAGCTGCT CACCCAAAGG
    AACAGAGAAC AGGAGAACAT CGTCCTGCTC AAAGCACGGA AGAAAACTCT CGAGTTTGAG
    CTGGAGGCTC TGAATGATAA GAAGTCTCAG TTGGAGGGGA AACTGCAGGA TATTCGGTCC
    CGTCTCTCGA TTCAGAGGCA TGAGATCGAG ACCACCAATA AGACTCGGGA GCTCCGCATT
    GCAGAGATCA CAAGCCTGCA ACAGCAGCTG CAGGAGTCTC AGCAGTGGTT GAGTCGCTTG
    ATTCCTGATA AACAGTGTCT GAATGACCAG CTGAAGCAGG TTCAGCAGAA CAGTCTGCAC
    AGAGACTCTT TGAACAGTCT TCAGAGGGCC ATTGAAATGA AAGAATCCAC CAAACAGCAG
    CTGAAAGAAC AGCTGGATGC TGTGGAGAAG GAAACTCGCT CCAAACTATT GGAGATCGAC
    GCCTTCAACA CACAGCTGAA GGAGCTGAGA GAAATCCACA ACAAACAGCA GAGGCAGAAA
    CAGAAGGAGC TGGAGGCTGA CATCACCCAG CCGTCACATG ACAGGAAGTC CATAGACTCA
    CAAGACTCGA GGTTGTCGGG GACTGATGAT GGCGTGTCTC CAGCGTGGAG GGATGATGGG
    CTGGGTAAAG CTCCTACTCC TCCTGTGTCT CAGGCCTGGA TGAGCCGGGT GAGTGAAGAA
    GAAAACCACA GCCGAGCTGA AATACAGGAT AACCTCTCCA AGCTTTTCAC CCAACATCCA
    CAAGCAGGAA AAATACAGGC TCAGTCTCCC TGGTCCATGG CAGATAAGAT CCCAGTGTCT
    GGTTTTAATC AGGAGAAGGT AAAGGTGGTC TACTATAGAG CAATGTATCC CTTTGAGGCC
    CGCAGTCATG ATGAGATCAC CATCCATCCT GGAGACATAG TCATGGTGAA GGGAGAGTGG
    GTGGATGAGT CTCAGACAGG TGAGCCTGGA TGGTTAGGAG GAGAGATTAA AGGGAAGACT
    GGCTGGTTTC CTGCCAACTA TGCGGAGAAG ATTCCAGAAT CCGAAGTTCC TCTGAGTTTG
    AGGGCCAGCG CTGCTTCTAG CTCCGCCCCT AAATTGGGTT CTCACATGTC ATCTGCTTCT
    TCATCGACCA CGGCCACGCC CATTCTGCCA GTCTCCACAG AGCCTGCATC GATAGCCCCG
    CCCTCTTCAG CCCCACCCCC CACAGGCCCC GCCTCCTCCT CTTCCTCAGC ATCAAGCAAT
    TGGGCAGATT TCAGCACTAC ATGGCCCTCT AATTCTGCTG TGGAGAAGCA GGATAGTGAT
    GGATGGGATG CATGGCCGAC TCAGCCAACT CAGCCTTCTC TGTCAGTGCC CAGTGGAGGG
    CAGATCAGAC AGCGCTCTGC TTTCACGCCT GCTACACTCT CCGGCTCCTC CCCCTCACCC
    GTGCTTGGAC AGGGTGAGAA GGTGGAGGGT CTGCAGGCTC AGGCGCTGTA CCCATGGAGA
    GCCAAGAAAG ACAATCATCT AAACTTCAAC AAGAATGACG TGATCACTGT ACTGGAGCAG
    CAGGACATGT GGTGGTTTGG GGAGGTGCAA GGACAGAGAG GATGGTTCCC TAAATCTTAC
    GTCAAGCTCA TCTCTGGACC TGTCCGGAAA TCTATGAGTA TTGAGTCTGG CTCTTCAGAC
    AGCCCTCCTA GTGTAAAGAG ACCCAGTCCC TCTCTAAACA AACCCACAGA GCTCGGAGAA
    GGTCAGAACT CCAACAGCAA CTCAATGTAT CCGTCTAAAG AGTATGTGGC CATGTATACA
    TATGAAAGCA ATGAGCAGGG TGACTTGACC TTCCAGCAAG GTGATGTCAT CACAGTCACC
    AAGAAAGAAG GAGATTGGTG GACCGGCACT GTGAGCGGGA AGATCGGAGT CTTTCCATCG
    AATTATGTCA AACCTAAAGA ATCTGAGGGG TTGGGATCTG CTGGGAAAAC AGGAAGTTTA
    GGGAAGAAAC CAGAGATTGC CCAAGTCATC GCTCCATACA CGGCCACTGG TGCAGAGCAG
    CTCACTTTAG CACCAGGCCA GCTCATTCTC ATTCGCAAGA AAAACCCGGG AGGATGGTGG
    GAAGGAGAAC TTCAGGCAAG AGGAAAGAAG CGGCAGATTG GCTGGTTTCC TGCAAATTAT
    GTCAAATTAT TGAGTCCCAG TACCAGTAAG ACAACACCCA CAGATCCCAA CCCACCCAAG
    CTGCCGACAC CCAATGCAGT GTGCCAGGTG ATTGGCATGT ATGATTACAC TGCTCAGAAT
    GATGACGAGC TGCCTTTCGG GAAGGGCCAA ATCATCAATG TGTTGAGCAG AGAGGATCCC
    GATTGGTGGA AAGGAGAGTT GAATGGATCC GTCGGCCTCT TCCCCTCGAA CTACGTCAAG
    CTGACCACCG ACACAGACCC CAGCCAACAA TGGTGTGCTG ATCTCCACCT GCTCGACATG
    CTGACCCCAG TGGAGCGGAA GAGGCAGGGA TACATACATG AGCTTATTGT TACAGAGGAG
    AACTATGTCA ATGACCTGCA GCTGGTCACT GAGACCTTCC AGAAGCCTCT GCTGGAGTCT
    GATTTGTTGA CGGAGAAGGA GGTTGCCATG ATCTTTGTCA ACTGGAAAGA GCTGACCATG
    TGCAACATTA AACTGCTCAA GGCGCTGCGG GTGAGGAAGA AGATGTCAGG TGAGCGGATG
    CCAGTGAAGA TGATTGGTGA CATCCTGACG GCTCAGCTTC CTCACATGCA GCCATACATC
    CGCTTCTGCA GCTGCCAGCT CAACGGCGCC ACCCTCATTC AGCAGAAAAC TGACGAGGTG
    CCCGAATTCA AGGACTTTGT GAAGAGGTTA GCGATGGACC CTCGCTGTAA GGGAATGCCC
    CTGTCCAGTT TCCTTCTCAA ACCCATGCAG AGAGTCACCC GATACCCCCT CATCATCAAA
    AACATTTTAG AGAACACTCC AGAAAGCCAC CCGGACCACA GTCACCTGCG TCTGGCTCTG
    GAGAAAGCAG AAGAGCTGTG CTCGCAGGTC AATGAAGGTG TACGAGAGAA AGAAAACTCC
    GACCGGCTGG AATGGATACA GGCACATGTG CAGTGTGAGG GACTGTCAGA GCAACTTGTG
    TTCAACTCTG TAACAAACTG TCTGGGCCCT CGGAAGTTCC TGCACAGTGG AAAACTCTAC
    AAAGCCAAGA GCAACAAAGA GCTTTACGGC TTCCTTTTTA ACGACTTCCT GCTCCTCACA
    CAGATAATCA AACCTCTGGG CTCATCCGGG ACAGACAAGG TCTTCAGTGC GAAATCCCAT
    CTGCAGTATC GCATGTACAA AACGCCCATC TTTCTCAATG AGGTGCTAGT GAAGCTGCCT
    ACTGACCCCT CGGGAGATGA ACCCATTTTT CACATATCTC ACATTGACAG AGTCTACACT
    ATACGGGCTG AGAGCATCAA CGAAAGGACA GCTTGGGTGC AGAAAATCAA AGCTGCCTCT
    GAGCTCTTCA TCGAAACCGA AAAGAGGAAG AGAGAGAAAG CCTACTTGGT GAGGTCTCAG
    AGGGCCACTG GCATCGGGCG GCTAATGGTC AACATTGTTG AAGGCATCGA ACTGAAGCCC
    TGCCGCTCAA ATGGTAAGAG TAACCCGTAC TGTGAGGTGA CGATGGGCTC TCAGTGTCAC
    GTCACCAAGA CCCTTCAGGA CACTCTGAAC CCCAAGTGGA ACTCAAACTG CCAGTTCTTC
    ATCAAAGACC TGGAGCAGGA CGTGCTGTGT GTCACTGTGT TTGAGAGGGA TCAGTTCTCC
    CCGGACGATT TCTTGGGCAG GACGGAGATT CGGCTGGCCG ACATCAAGAA GGACCAGGGC
    TCGAAGGGTC CCATCACCAA GCGTCTGCTT TTACACGAAG TCCCGACGGG AGAGATTGTG
    GTGCGACTGG ACCTGCAGCT CTTCGATGAA CCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa06107
    Clone ID Related Accession (Same CDS sequence) XM_005170972.3 , XM_005170972.4
    Accession Version XM_005170972.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5241bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product intersectin-1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005170972.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCTCAGT TTCCCACACC CTTCGGAGGG GGGTCGGATA CATGGGTAAT ATCTGTGGAC 
    GAGAGAGCCA AACATGACCA GCAGTTTCAC AGTCTCACCC CAACACCCGC TGGCTTCATC
    ACAGGTGACC AGGCAAGGAA TTTCTTCCTG CAGTCAGGTT TACCTGCCCC CATCTTGGCC
    CAGATATGGG CTCTGGCCGA CATGAACAAT GACGGGAAGA TGGACATGCA TGAGTTTTCC
    ATCGCAATGA AGCTGATTAA ATTAAAGCTG CAGGGTCACC CTCTTCCTCC ATCACTGCCC
    CCCTCCATGA AGCAACAGCC CATCTCCATA CCCCCGCCGA CCCCTTTTGG GATACCCTCT
    ATGGGTGCAA TGCCAGGCCT GCCAGCAGTG CCGCCCATGC CTCTGCCCCC TCTGCCTCCA
    GGAGTGCCAG GGATGGGTGT TTCTCCTCCA CTGGGCGCAT CGGTAACCCC ACCCGTACCA
    TCCCTCGCCA ACGGAGCACC CGCAATGATA CAACCCCTGA GTGGATTTTC ACATCCAGGT
    GCCGTGCTCA ACAAAACCCC CTCATTCAAT CGCTCCAGTA TCAAGCTACA GAAGGGCCAG
    TCTGTAGAGG CGCCCAGTGC TCCAGCTGCT CCTCCACCCG CTGACTGGGC CGTGTCTCAG
    TCCTCCAGAC TTAAGTATCG CCAGCTCTTC AACAGCCATG ATAAAATGAT GAGCGGTTAT
    CTCACAGGTC CACAAGCCAG AACTATTCTG ATGCAGTCCA GTCTACCACA AGCCCAGCTG
    GCCACCATCT GGAATCTGTC AGATATAGAT CAGGATGGGA AGTTGACCGC TGAAGAGTTT
    ATTTTGGCCA TGCACTTGAT CGACATGGCC ATGTCTGGTC TCCCTCTTCC CCCTTTGCTG
    CCGCCTGACC TTATACCTCC AACATTCAGG AGAATGCGCT CTGGTAGTGG AGTGTCTGTG
    ACTAGCATGC ACTCGACAGA CTTGCGGTCT CAGGAAGAGC CAGAGGAGGA GGAGAAAGAG
    GCCGAGAAAA AACTTCCAGT CACATTTGAG GATAAGAAAC GAGAGAACTT TGAGCGCGGA
    AACCTGGAGT TGGAGAAACG TCGACAGGCA CTTCTGGAGC AGCAGAGGAA AGAGCAGGAG
    CGACTGGCGG CGCTGGAGAA AGAAGAGCAG GAGCGGAAGG AGAGGGAACG ACTGGAGCAG
    GAGAGACGCA GACAGCAGGA GCTGGACAAA CAGCTGGAGA GACAGAGGGA GCTGGAGCGA
    CAGCGGGAGG AGGAGAGACG CAAGGAGATC GAGAGAAGAG AGGCTGCTAA ACGGGAGCTG
    GAGCGACAGA GGCAGCTGGA GTGGGAGAGG ACGCGCAGAC AGGAGCTGCT CACCCAAAGG
    AACAGAGAAC AGGAGAACAT CGTCCTGCTC AAAGCACGGA AGAAAACTCT CGAGTTTGAG
    CTGGAGGCTC TGAATGATAA GAAGTCTCAG TTGGAGGGGA AACTGCAGGA TATTCGGTCC
    CGTCTCTCGA TTCAGAGGCA TGAGATCGAG ACCACCAATA AGACTCGGGA GCTCCGCATT
    GCAGAGATCA CAAGCCTGCA ACAGCAGCTG CAGGAGTCTC AGCAGTGGTT GAGTCGCTTG
    ATTCCTGATA AACAGTGTCT GAATGACCAG CTGAAGCAGG TTCAGCAGAA CAGTCTGCAC
    AGAGACTCTT TGAACAGTCT TCAGAGGGCC ATTGAAATGA AAGAATCCAC CAAACAGCAG
    CTGAAAGAAC AGCTGGATGC TGTGGAGAAG GAAACTCGCT CCAAACTATT GGAGATCGAC
    GCCTTCAACA CACAGCTGAA GGAGCTGAGA GAAATCCACA ACAAACAGCA GAGGCAGAAA
    CAGAAGGAGC TGGAGGCTGA CATCACCCAG CCGTCACATG ACAGGAAGTC CATAGACTCA
    CAAGACTCGA GGTTGTCGGG GACTGATGAT GGCGTGTCTC CAGCGTGGAG GGATGATGGG
    CTGGGTAAAG CTCCTACTCC TCCTGTGTCT CAGGCCTGGA TGAGCCGGGT GAGTGAAGAA
    GAAAACCACA GCCGAGCTGA AATACAGGAT AACCTCTCCA AGCTTTTCAC CCAACATCCA
    CAAGCAGGAA AAATACAGGC TCAGTCTCCC TGGTCCATGG CAGATAAGAT CCCAGTGTCT
    GGTTTTAATC AGGAGAAGGT AAAGGTGGTC TACTATAGAG CAATGTATCC CTTTGAGGCC
    CGCAGTCATG ATGAGATCAC CATCCATCCT GGAGACATAG TCATGGTGGA TGAGTCTCAG
    ACAGGTGAGC CTGGATGGTT AGGAGGAGAG ATTAAAGGGA AGACTGGCTG GTTTCCTGCC
    AACTATGCGG AGAAGATTCC AGAATCCGAA GTTCCTCTGA GTTTGAGGGC CAGCGCTGCT
    TCTAGCTCCG CCCCTAAATT GGGTTCTCAC ATGTCATCTG CTTCTTCATC GACCACGGCC
    ACGCCCATTC TGCCAGTCTC CACAGAGCCT GCATCGATAG CCCCGCCCTC TTCAGCCCCA
    CCCCCCACAG GCCCCGCCTC CTCCTCTTCC TCAGCATCAA GCAATTGGGC AGATTTCAGC
    ACTACATGGC CCTCTAATTC TGCTGTGGAG AAGCAGGATA GTGATGGATG GGATGCATGG
    CCGACTCAGC CAACTCAGCC TTCTCTGTCA GTGCCCAGTG GAGGGCAGAT CAGACAGCGC
    TCTGCTTTCA CGCCTGCTAC ACTCTCCGGC TCCTCCCCCT CACCCGTGCT TGGACAGGGT
    GAGAAGGTGG AGGGTCTGCA GGCTCAGGCG CTGTACCCAT GGAGAGCCAA GAAAGACAAT
    CATCTAAACT TCAACAAGAA TGACGTGATC ACTGTACTGG AGCAGCAGGA CATGTGGTGG
    TTTGGGGAGG TGCAAGGACA GAGAGGATGG TTCCCTAAAT CTTACGTCAA GCTCATCTCT
    GGACCTGTCC GGAAATCTAT GAGTATTGAG TCTGGCTCTT CAGACAGCCC TCCTAGTGTA
    AAGAGACCCA GTCCCTCTCT AAACAAACCC ACAGAGCTCG GAGAAGGTCA GAACTCCAAC
    AGCAACTCAA TGTATCCGTC TAAAGAGTAT GTGGCCATGT ATACATATGA AAGCAATGAG
    CAGGGTGACT TGACCTTCCA GCAAGGTGAT GTCATCACAG TCACCAAGAA AGAAGGAGAT
    TGGTGGACCG GCACTGTGAG CGGGAAGATC GGAGTCTTTC CATCGAATTA TGTCAAACCT
    AAAGAATCTG AGGGGTTGGG ATCTGCTGGG AAAACAGGAA GTTTAGGGAA GAAACCAGAG
    ATTGCCCAAG TCATCGCTCC ATACACGGCC ACTGGTGCAG AGCAGCTCAC TTTAGCACCA
    GGCCAGCTCA TTCTCATTCG CAAGAAAAAC CCGGGAGGAT GGTGGGAAGG AGAACTTCAG
    GCAAGAGGAA AGAAGCGGCA GATTGGCTGG TTTCCTGCAA ATTATGTCAA ATTATTGAGT
    CCCAGTACCA GTAAGACAAC ACCCACAGAT CCCAACCCAC CCAAGCTGCC GACACCCAAT
    GCAGTGTGCC AGGTGATTGG CATGTATGAT TACACTGCTC AGAATGATGA CGAGCTGCCT
    TTCGGGAAGG GCCAAATCAT CAATGTGTTG AGCAGAGAGG ATCCCGATTG GTGGAAAGGA
    GAGTTGAATG GATCCGTCGG CCTCTTCCCC TCGAACTACG TCAAGCTGAC CACCGACACA
    GACCCCAGCC AACAATGGTG TGCTGATCTC CACCTGCTCG ACATGCTGAC CCCAGTGGAG
    CGGAAGAGGC AGGGATACAT ACATGAGCTT ATTGTTACAG AGGAGAACTA TGTCAATGAC
    CTGCAGCTGG TCACTGAGAC CTTCCAGAAG CCTCTGCTGG AGTCTGATTT GTTGACGGAG
    AAGGAGGTTG CCATGATCTT TGTCAACTGG AAAGAGCTGA CCATGTGCAA CATTAAACTG
    CTCAAGGCGC TGCGGGTGAG GAAGAAGATG TCAGGTGAGC GGATGCCAGT GAAGATGATT
    GGTGACATCC TGACGGCTCA GCTTCCTCAC ATGCAGCCAT ACATCCGCTT CTGCAGCTGC
    CAGCTCAACG GCGCCACCCT CATTCAGCAG AAAACTGACG AGGTGCCCGA ATTCAAGGAC
    TTTGTGAAGA GGTTAGCGAT GGACCCTCGC TGTAAGGGAA TGCCCCTGTC CAGTTTCCTT
    CTCAAACCCA TGCAGAGAGT CACCCGATAC CCCCTCATCA TCAAAAACAT TTTAGAGAAC
    ACTCCAGAAA GCCACCCGGA CCACAGTCAC CTGCGTCTGG CTCTGGAGAA AGCAGAAGAG
    CTGTGCTCGC AGGTCAATGA AGGTGTACGA GAGAAAGAAA ACTCCGACCG GCTGGAATGG
    ATACAGGCAC ATGTGCAGTG TGAGGGACTG TCAGAGCAAC TTGTGTTCAA CTCTGTAACA
    AACTGTCTGG GCCCTCGGAA GTTCCTGCAC AGTGGAAAAC TCTACAAAGC CAAGAGCAAC
    AAAGAGCTTT ACGGCTTCCT TTTTAACGAC TTCCTGCTCC TCACACAGAT AATCAAACCT
    CTGGGCTCAT CCGGGACAGA CAAGGTCTTC AGTGCGAAAT CCCATCTGCA GTATCGCATG
    TACAAAACGC CCATCTTTCT CAATGAGGTG CTAGTGAAGC TGCCTACTGA CCCCTCGGGA
    GATGAACCCA TTTTTCACAT ATCTCACATT GACAGAGTCT ACACTATACG GGCTGAGAGC
    ATCAACGAAA GGACAGCTTG GGTGCAGAAA ATCAAAGCTG CCTCTGAGCT CTTCATCGAA
    ACCGAAAAGA GGAAGAGAGA GAAAGCCTAC TTGGTGAGGT CTCAGAGGGC CACTGGCATC
    GGGCGGCTAA TGGTCAACAT TGTTGAAGGC ATCGAACTGA AGCCCTGCCG CTCAAATGGT
    AAGAGTAACC CGTACTGTGA GGTGACGATG GGCTCTCAGT GTCACGTCAC CAAGACCCTT
    CAGGACACTC TGAACCCCAA GTGGAACTCA AACTGCCAGT TCTTCATCAA AGACCTGGAG
    CAGGACGTGC TGTGTGTCAC TGTGTTTGAG AGGGATCAGT TCTCCCCGGA CGATTTCTTG
    GGCAGGACGG AGATTCGGCT GGCCGACATC AAGAAGGACC AGGGCTCGAA GGGTCCCATC
    ACCAAGCGTC TGCTTTTACA CGAAGTCCCG ACGGGAGAGA TTGTGGTGCG ACTGGACCTG
    CAGCTCTTCG ATGAACCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005171029.1
    CDS123..5363
    Translation

    Target ORF information:

    RefSeq Version XM_005170972.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio intersectin 1 (SH3 domain protein) (itsn1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005170972.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCTCAGT TTCCCACACC CTTCGGAGGG GGGTCGGATA CATGGGTAAT ATCTGTGGAC 
    GAGAGAGCCA AACATGACCA GCAGTTTCAC AGTCTCACCC CAACACCCGC TGGCTTCATC
    ACAGGTGACC AGGCAAGGAA TTTCTTCCTG CAGTCAGGTT TACCTGCCCC CATCTTGGCC
    CAGATATGGG CTCTGGCCGA CATGAACAAT GACGGGAAGA TGGACATGCA TGAGTTTTCC
    ATCGCAATGA AGCTGATTAA ATTAAAGCTG CAGGGTCACC CTCTTCCTCC ATCACTGCCC
    CCCTCCATGA AGCAACAGCC CATCTCCATA CCCCCGCCGA CCCCTTTTGG GATACCCTCT
    ATGGGTGCAA TGCCAGGCCT GCCAGCAGTG CCGCCCATGC CTCTGCCCCC TCTGCCTCCA
    GGAGTGCCAG GGATGGGTGT TTCTCCTCCA CTGGGCGCAT CGGTAACCCC ACCCGTACCA
    TCCCTCGCCA ACGGAGCACC CGCAATGATA CAACCCCTGA GTGGATTTTC ACATCCAGGT
    GCCGTGCTCA ACAAAACCCC CTCATTCAAT CGCTCCAGTA TCAAGCTACA GAAGGGCCAG
    TCTGTAGAGG CGCCCAGTGC TCCAGCTGCT CCTCCACCCG CTGACTGGGC CGTGTCTCAG
    TCCTCCAGAC TTAAGTATCG CCAGCTCTTC AACAGCCATG ATAAAATGAT GAGCGGTTAT
    CTCACAGGTC CACAAGCCAG AACTATTCTG ATGCAGTCCA GTCTACCACA AGCCCAGCTG
    GCCACCATCT GGAATCTGTC AGATATAGAT CAGGATGGGA AGTTGACCGC TGAAGAGTTT
    ATTTTGGCCA TGCACTTGAT CGACATGGCC ATGTCTGGTC TCCCTCTTCC CCCTTTGCTG
    CCGCCTGACC TTATACCTCC AACATTCAGG AGAATGCGCT CTGGTAGTGG AGTGTCTGTG
    ACTAGCATGC ACTCGACAGA CTTGCGGTCT CAGGAAGAGC CAGAGGAGGA GGAGAAAGAG
    GCCGAGAAAA AACTTCCAGT CACATTTGAG GATAAGAAAC GAGAGAACTT TGAGCGCGGA
    AACCTGGAGT TGGAGAAACG TCGACAGGCA CTTCTGGAGC AGCAGAGGAA AGAGCAGGAG
    CGACTGGCGG CGCTGGAGAA AGAAGAGCAG GAGCGGAAGG AGAGGGAACG ACTGGAGCAG
    GAGAGACGCA GACAGCAGGA GCTGGACAAA CAGCTGGAGA GACAGAGGGA GCTGGAGCGA
    CAGCGGGAGG AGGAGAGACG CAAGGAGATC GAGAGAAGAG AGGCTGCTAA ACGGGAGCTG
    GAGCGACAGA GGCAGCTGGA GTGGGAGAGG ACGCGCAGAC AGGAGCTGCT CACCCAAAGG
    AACAGAGAAC AGGAGAACAT CGTCCTGCTC AAAGCACGGA AGAAAACTCT CGAGTTTGAG
    CTGGAGGCTC TGAATGATAA GAAGTCTCAG TTGGAGGGGA AACTGCAGGA TATTCGGTCC
    CGTCTCTCGA TTCAGAGGCA TGAGATCGAG ACCACCAATA AGACTCGGGA GCTCCGCATT
    GCAGAGATCA CAAGCCTGCA ACAGCAGCTG CAGGAGTCTC AGCAGTGGTT GAGTCGCTTG
    ATTCCTGATA AACAGTGTCT GAATGACCAG CTGAAGCAGG TTCAGCAGAA CAGTCTGCAC
    AGAGACTCTT TGAACAGTCT TCAGAGGGCC ATTGAAATGA AAGAATCCAC CAAACAGCAG
    CTGAAAGAAC AGCTGGATGC TGTGGAGAAG GAAACTCGCT CCAAACTATT GGAGATCGAC
    GCCTTCAACA CACAGCTGAA GGAGCTGAGA GAAATCCACA ACAAACAGCA GAGGCAGAAA
    CAGAAGGAGC TGGAGGCTGA CATCACCCAG CCGTCACATG ACAGGAAGTC CATAGACTCA
    CAAGACTCGA GGTTGTCGGG GACTGATGAT GGCGTGTCTC CAGCGTGGAG GGATGATGGG
    CTGGGTAAAG CTCCTACTCC TCCTGTGTCT CAGGCCTGGA TGAGCCGGGT GAGTGAAGAA
    GAAAACCACA GCCGAGCTGA AATACAGGAT AACCTCTCCA AGCTTTTCAC CCAACATCCA
    CAAGCAGGAA AAATACAGGC TCAGTCTCCC TGGTCCATGG CAGATAAGAT CCCAGTGTCT
    GGTTTTAATC AGGAGAAGGT AAAGGTGGTC TACTATAGAG CAATGTATCC CTTTGAGGCC
    CGCAGTCATG ATGAGATCAC CATCCATCCT GGAGACATAG TCATGGTGGA TGAGTCTCAG
    ACAGGTGAGC CTGGATGGTT AGGAGGAGAG ATTAAAGGGA AGACTGGCTG GTTTCCTGCC
    AACTATGCGG AGAAGATTCC AGAATCCGAA GTTCCTCTGA GTTTGAGGGC CAGCGCTGCT
    TCTAGCTCCG CCCCTAAATT GGGTTCTCAC ATGTCATCTG CTTCTTCATC GACCACGGCC
    ACGCCCATTC TGCCAGTCTC CACAGAGCCT GCATCGATAG CCCCGCCCTC TTCAGCCCCA
    CCCCCCACAG GCCCCGCCTC CTCCTCTTCC TCAGCATCAA GCAATTGGGC AGATTTCAGC
    ACTACATGGC CCTCTAATTC TGCTGTGGAG AAGCAGGATA GTGATGGATG GGATGCATGG
    CCGACTCAGC CAACTCAGCC TTCTCTGTCA GTGCCCAGTG GAGGGCAGAT CAGACAGCGC
    TCTGCTTTCA CGCCTGCTAC ACTCTCCGGC TCCTCCCCCT CACCCGTGCT TGGACAGGGT
    GAGAAGGTGG AGGGTCTGCA GGCTCAGGCG CTGTACCCAT GGAGAGCCAA GAAAGACAAT
    CATCTAAACT TCAACAAGAA TGACGTGATC ACTGTACTGG AGCAGCAGGA CATGTGGTGG
    TTTGGGGAGG TGCAAGGACA GAGAGGATGG TTCCCTAAAT CTTACGTCAA GCTCATCTCT
    GGACCTGTCC GGAAATCTAT GAGTATTGAG TCTGGCTCTT CAGACAGCCC TCCTAGTGTA
    AAGAGACCCA GTCCCTCTCT AAACAAACCC ACAGAGCTCG GAGAAGGTCA GAACTCCAAC
    AGCAACTCAA TGTATCCGTC TAAAGAGTAT GTGGCCATGT ATACATATGA AAGCAATGAG
    CAGGGTGACT TGACCTTCCA GCAAGGTGAT GTCATCACAG TCACCAAGAA AGAAGGAGAT
    TGGTGGACCG GCACTGTGAG CGGGAAGATC GGAGTCTTTC CATCGAATTA TGTCAAACCT
    AAAGAATCTG AGGGGTTGGG ATCTGCTGGG AAAACAGGAA GTTTAGGGAA GAAACCAGAG
    ATTGCCCAAG TCATCGCTCC ATACACGGCC ACTGGTGCAG AGCAGCTCAC TTTAGCACCA
    GGCCAGCTCA TTCTCATTCG CAAGAAAAAC CCGGGAGGAT GGTGGGAAGG AGAACTTCAG
    GCAAGAGGAA AGAAGCGGCA GATTGGCTGG TTTCCTGCAA ATTATGTCAA ATTATTGAGT
    CCCAGTACCA GTAAGACAAC ACCCACAGAT CCCAACCCAC CCAAGCTGCC GACACCCAAT
    GCAGTGTGCC AGGTGATTGG CATGTATGAT TACACTGCTC AGAATGATGA CGAGCTGCCT
    TTCGGGAAGG GCCAAATCAT CAATGTGTTG AGCAGAGAGG ATCCCGATTG GTGGAAAGGA
    GAGTTGAATG GATCCGTCGG CCTCTTCCCC TCGAACTACG TCAAGCTGAC CACCGACACA
    GACCCCAGCC AACAATGGTG TGCTGATCTC CACCTGCTCG ACATGCTGAC CCCAGTGGAG
    CGGAAGAGGC AGGGATACAT ACATGAGCTT ATTGTTACAG AGGAGAACTA TGTCAATGAC
    CTGCAGCTGG TCACTGAGAC CTTCCAGAAG CCTCTGCTGG AGTCTGATTT GTTGACGGAG
    AAGGAGGTTG CCATGATCTT TGTCAACTGG AAAGAGCTGA CCATGTGCAA CATTAAACTG
    CTCAAGGCGC TGCGGGTGAG GAAGAAGATG TCAGGTGAGC GGATGCCAGT GAAGATGATT
    GGTGACATCC TGACGGCTCA GCTTCCTCAC ATGCAGCCAT ACATCCGCTT CTGCAGCTGC
    CAGCTCAACG GCGCCACCCT CATTCAGCAG AAAACTGACG AGGTGCCCGA ATTCAAGGAC
    TTTGTGAAGA GGTTAGCGAT GGACCCTCGC TGTAAGGGAA TGCCCCTGTC CAGTTTCCTT
    CTCAAACCCA TGCAGAGAGT CACCCGATAC CCCCTCATCA TCAAAAACAT TTTAGAGAAC
    ACTCCAGAAA GCCACCCGGA CCACAGTCAC CTGCGTCTGG CTCTGGAGAA AGCAGAAGAG
    CTGTGCTCGC AGGTCAATGA AGGTGTACGA GAGAAAGAAA ACTCCGACCG GCTGGAATGG
    ATACAGGCAC ATGTGCAGTG TGAGGGACTG TCAGAGCAAC TTGTGTTCAA CTCTGTAACA
    AACTGTCTGG GCCCTCGGAA GTTCCTGCAC AGTGGAAAAC TCTACAAAGC CAAGAGCAAC
    AAAGAGCTTT ACGGCTTCCT TTTTAACGAC TTCCTGCTCC TCACACAGAT AATCAAACCT
    CTGGGCTCAT CCGGGACAGA CAAGGTCTTC AGTGCGAAAT CCCATCTGCA GTATCGCATG
    TACAAAACGC CCATCTTTCT CAATGAGGTG CTAGTGAAGC TGCCTACTGA CCCCTCGGGA
    GATGAACCCA TTTTTCACAT ATCTCACATT GACAGAGTCT ACACTATACG GGCTGAGAGC
    ATCAACGAAA GGACAGCTTG GGTGCAGAAA ATCAAAGCTG CCTCTGAGCT CTTCATCGAA
    ACCGAAAAGA GGAAGAGAGA GAAAGCCTAC TTGGTGAGGT CTCAGAGGGC CACTGGCATC
    GGGCGGCTAA TGGTCAACAT TGTTGAAGGC ATCGAACTGA AGCCCTGCCG CTCAAATGGT
    AAGAGTAACC CGTACTGTGA GGTGACGATG GGCTCTCAGT GTCACGTCAC CAAGACCCTT
    CAGGACACTC TGAACCCCAA GTGGAACTCA AACTGCCAGT TCTTCATCAA AGACCTGGAG
    CAGGACGTGC TGTGTGTCAC TGTGTTTGAG AGGGATCAGT TCTCCCCGGA CGATTTCTTG
    GGCAGGACGG AGATTCGGCT GGCCGACATC AAGAAGGACC AGGGCTCGAA GGGTCCCATC
    ACCAAGCGTC TGCTTTTACA CGAAGTCCCG ACGGGAGAGA TTGTGGTGCG ACTGGACCTG
    CAGCTCTTCG ATGAACCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa06107
    Clone ID Related Accession (Same CDS sequence) XM_005170972.3 , XM_005170972.4
    Accession Version XM_005170972.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5241bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product intersectin-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005170972.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCTCAGT TTCCCACACC CTTCGGAGGG GGGTCGGATA CATGGGTAAT ATCTGTGGAC 
    GAGAGAGCCA AACATGACCA GCAGTTTCAC AGTCTCACCC CAACACCCGC TGGCTTCATC
    ACAGGTGACC AGGCAAGGAA TTTCTTCCTG CAGTCAGGTT TACCTGCCCC CATCTTGGCC
    CAGATATGGG CTCTGGCCGA CATGAACAAT GACGGGAAGA TGGACATGCA TGAGTTTTCC
    ATCGCAATGA AGCTGATTAA ATTAAAGCTG CAGGGTCACC CTCTTCCTCC ATCACTGCCC
    CCCTCCATGA AGCAACAGCC CATCTCCATA CCCCCGCCGA CCCCTTTTGG GATACCCTCT
    ATGGGTGCAA TGCCAGGCCT GCCAGCAGTG CCGCCCATGC CTCTGCCCCC TCTGCCTCCA
    GGAGTGCCAG GGATGGGTGT TTCTCCTCCA CTGGGCGCAT CGGTAACCCC ACCCGTACCA
    TCCCTCGCCA ACGGAGCACC CGCAATGATA CAACCCCTGA GTGGATTTTC ACATCCAGGT
    GCCGTGCTCA ACAAAACCCC CTCATTCAAT CGCTCCAGTA TCAAGCTACA GAAGGGCCAG
    TCTGTAGAGG CGCCCAGTGC TCCAGCTGCT CCTCCACCCG CTGACTGGGC CGTGTCTCAG
    TCCTCCAGAC TTAAGTATCG CCAGCTCTTC AACAGCCATG ATAAAATGAT GAGCGGTTAT
    CTCACAGGTC CACAAGCCAG AACTATTCTG ATGCAGTCCA GTCTACCACA AGCCCAGCTG
    GCCACCATCT GGAATCTGTC AGATATAGAT CAGGATGGGA AGTTGACCGC TGAAGAGTTT
    ATTTTGGCCA TGCACTTGAT CGACATGGCC ATGTCTGGTC TCCCTCTTCC CCCTTTGCTG
    CCGCCTGACC TTATACCTCC AACATTCAGG AGAATGCGCT CTGGTAGTGG AGTGTCTGTG
    ACTAGCATGC ACTCGACAGA CTTGCGGTCT CAGGAAGAGC CAGAGGAGGA GGAGAAAGAG
    GCCGAGAAAA AACTTCCAGT CACATTTGAG GATAAGAAAC GAGAGAACTT TGAGCGCGGA
    AACCTGGAGT TGGAGAAACG TCGACAGGCA CTTCTGGAGC AGCAGAGGAA AGAGCAGGAG
    CGACTGGCGG CGCTGGAGAA AGAAGAGCAG GAGCGGAAGG AGAGGGAACG ACTGGAGCAG
    GAGAGACGCA GACAGCAGGA GCTGGACAAA CAGCTGGAGA GACAGAGGGA GCTGGAGCGA
    CAGCGGGAGG AGGAGAGACG CAAGGAGATC GAGAGAAGAG AGGCTGCTAA ACGGGAGCTG
    GAGCGACAGA GGCAGCTGGA GTGGGAGAGG ACGCGCAGAC AGGAGCTGCT CACCCAAAGG
    AACAGAGAAC AGGAGAACAT CGTCCTGCTC AAAGCACGGA AGAAAACTCT CGAGTTTGAG
    CTGGAGGCTC TGAATGATAA GAAGTCTCAG TTGGAGGGGA AACTGCAGGA TATTCGGTCC
    CGTCTCTCGA TTCAGAGGCA TGAGATCGAG ACCACCAATA AGACTCGGGA GCTCCGCATT
    GCAGAGATCA CAAGCCTGCA ACAGCAGCTG CAGGAGTCTC AGCAGTGGTT GAGTCGCTTG
    ATTCCTGATA AACAGTGTCT GAATGACCAG CTGAAGCAGG TTCAGCAGAA CAGTCTGCAC
    AGAGACTCTT TGAACAGTCT TCAGAGGGCC ATTGAAATGA AAGAATCCAC CAAACAGCAG
    CTGAAAGAAC AGCTGGATGC TGTGGAGAAG GAAACTCGCT CCAAACTATT GGAGATCGAC
    GCCTTCAACA CACAGCTGAA GGAGCTGAGA GAAATCCACA ACAAACAGCA GAGGCAGAAA
    CAGAAGGAGC TGGAGGCTGA CATCACCCAG CCGTCACATG ACAGGAAGTC CATAGACTCA
    CAAGACTCGA GGTTGTCGGG GACTGATGAT GGCGTGTCTC CAGCGTGGAG GGATGATGGG
    CTGGGTAAAG CTCCTACTCC TCCTGTGTCT CAGGCCTGGA TGAGCCGGGT GAGTGAAGAA
    GAAAACCACA GCCGAGCTGA AATACAGGAT AACCTCTCCA AGCTTTTCAC CCAACATCCA
    CAAGCAGGAA AAATACAGGC TCAGTCTCCC TGGTCCATGG CAGATAAGAT CCCAGTGTCT
    GGTTTTAATC AGGAGAAGGT AAAGGTGGTC TACTATAGAG CAATGTATCC CTTTGAGGCC
    CGCAGTCATG ATGAGATCAC CATCCATCCT GGAGACATAG TCATGGTGGA TGAGTCTCAG
    ACAGGTGAGC CTGGATGGTT AGGAGGAGAG ATTAAAGGGA AGACTGGCTG GTTTCCTGCC
    AACTATGCGG AGAAGATTCC AGAATCCGAA GTTCCTCTGA GTTTGAGGGC CAGCGCTGCT
    TCTAGCTCCG CCCCTAAATT GGGTTCTCAC ATGTCATCTG CTTCTTCATC GACCACGGCC
    ACGCCCATTC TGCCAGTCTC CACAGAGCCT GCATCGATAG CCCCGCCCTC TTCAGCCCCA
    CCCCCCACAG GCCCCGCCTC CTCCTCTTCC TCAGCATCAA GCAATTGGGC AGATTTCAGC
    ACTACATGGC CCTCTAATTC TGCTGTGGAG AAGCAGGATA GTGATGGATG GGATGCATGG
    CCGACTCAGC CAACTCAGCC TTCTCTGTCA GTGCCCAGTG GAGGGCAGAT CAGACAGCGC
    TCTGCTTTCA CGCCTGCTAC ACTCTCCGGC TCCTCCCCCT CACCCGTGCT TGGACAGGGT
    GAGAAGGTGG AGGGTCTGCA GGCTCAGGCG CTGTACCCAT GGAGAGCCAA GAAAGACAAT
    CATCTAAACT TCAACAAGAA TGACGTGATC ACTGTACTGG AGCAGCAGGA CATGTGGTGG
    TTTGGGGAGG TGCAAGGACA GAGAGGATGG TTCCCTAAAT CTTACGTCAA GCTCATCTCT
    GGACCTGTCC GGAAATCTAT GAGTATTGAG TCTGGCTCTT CAGACAGCCC TCCTAGTGTA
    AAGAGACCCA GTCCCTCTCT AAACAAACCC ACAGAGCTCG GAGAAGGTCA GAACTCCAAC
    AGCAACTCAA TGTATCCGTC TAAAGAGTAT GTGGCCATGT ATACATATGA AAGCAATGAG
    CAGGGTGACT TGACCTTCCA GCAAGGTGAT GTCATCACAG TCACCAAGAA AGAAGGAGAT
    TGGTGGACCG GCACTGTGAG CGGGAAGATC GGAGTCTTTC CATCGAATTA TGTCAAACCT
    AAAGAATCTG AGGGGTTGGG ATCTGCTGGG AAAACAGGAA GTTTAGGGAA GAAACCAGAG
    ATTGCCCAAG TCATCGCTCC ATACACGGCC ACTGGTGCAG AGCAGCTCAC TTTAGCACCA
    GGCCAGCTCA TTCTCATTCG CAAGAAAAAC CCGGGAGGAT GGTGGGAAGG AGAACTTCAG
    GCAAGAGGAA AGAAGCGGCA GATTGGCTGG TTTCCTGCAA ATTATGTCAA ATTATTGAGT
    CCCAGTACCA GTAAGACAAC ACCCACAGAT CCCAACCCAC CCAAGCTGCC GACACCCAAT
    GCAGTGTGCC AGGTGATTGG CATGTATGAT TACACTGCTC AGAATGATGA CGAGCTGCCT
    TTCGGGAAGG GCCAAATCAT CAATGTGTTG AGCAGAGAGG ATCCCGATTG GTGGAAAGGA
    GAGTTGAATG GATCCGTCGG CCTCTTCCCC TCGAACTACG TCAAGCTGAC CACCGACACA
    GACCCCAGCC AACAATGGTG TGCTGATCTC CACCTGCTCG ACATGCTGAC CCCAGTGGAG
    CGGAAGAGGC AGGGATACAT ACATGAGCTT ATTGTTACAG AGGAGAACTA TGTCAATGAC
    CTGCAGCTGG TCACTGAGAC CTTCCAGAAG CCTCTGCTGG AGTCTGATTT GTTGACGGAG
    AAGGAGGTTG CCATGATCTT TGTCAACTGG AAAGAGCTGA CCATGTGCAA CATTAAACTG
    CTCAAGGCGC TGCGGGTGAG GAAGAAGATG TCAGGTGAGC GGATGCCAGT GAAGATGATT
    GGTGACATCC TGACGGCTCA GCTTCCTCAC ATGCAGCCAT ACATCCGCTT CTGCAGCTGC
    CAGCTCAACG GCGCCACCCT CATTCAGCAG AAAACTGACG AGGTGCCCGA ATTCAAGGAC
    TTTGTGAAGA GGTTAGCGAT GGACCCTCGC TGTAAGGGAA TGCCCCTGTC CAGTTTCCTT
    CTCAAACCCA TGCAGAGAGT CACCCGATAC CCCCTCATCA TCAAAAACAT TTTAGAGAAC
    ACTCCAGAAA GCCACCCGGA CCACAGTCAC CTGCGTCTGG CTCTGGAGAA AGCAGAAGAG
    CTGTGCTCGC AGGTCAATGA AGGTGTACGA GAGAAAGAAA ACTCCGACCG GCTGGAATGG
    ATACAGGCAC ATGTGCAGTG TGAGGGACTG TCAGAGCAAC TTGTGTTCAA CTCTGTAACA
    AACTGTCTGG GCCCTCGGAA GTTCCTGCAC AGTGGAAAAC TCTACAAAGC CAAGAGCAAC
    AAAGAGCTTT ACGGCTTCCT TTTTAACGAC TTCCTGCTCC TCACACAGAT AATCAAACCT
    CTGGGCTCAT CCGGGACAGA CAAGGTCTTC AGTGCGAAAT CCCATCTGCA GTATCGCATG
    TACAAAACGC CCATCTTTCT CAATGAGGTG CTAGTGAAGC TGCCTACTGA CCCCTCGGGA
    GATGAACCCA TTTTTCACAT ATCTCACATT GACAGAGTCT ACACTATACG GGCTGAGAGC
    ATCAACGAAA GGACAGCTTG GGTGCAGAAA ATCAAAGCTG CCTCTGAGCT CTTCATCGAA
    ACCGAAAAGA GGAAGAGAGA GAAAGCCTAC TTGGTGAGGT CTCAGAGGGC CACTGGCATC
    GGGCGGCTAA TGGTCAACAT TGTTGAAGGC ATCGAACTGA AGCCCTGCCG CTCAAATGGT
    AAGAGTAACC CGTACTGTGA GGTGACGATG GGCTCTCAGT GTCACGTCAC CAAGACCCTT
    CAGGACACTC TGAACCCCAA GTGGAACTCA AACTGCCAGT TCTTCATCAA AGACCTGGAG
    CAGGACGTGC TGTGTGTCAC TGTGTTTGAG AGGGATCAGT TCTCCCCGGA CGATTTCTTG
    GGCAGGACGG AGATTCGGCT GGCCGACATC AAGAAGGACC AGGGCTCGAA GGGTCCCATC
    ACCAAGCGTC TGCTTTTACA CGAAGTCCCG ACGGGAGAGA TTGTGGTGCG ACTGGACCTG
    CAGCTCTTCG ATGAACCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005171029.1
    CDS227..5467
    Translation

    Target ORF information:

    RefSeq Version XM_005170972.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio intersectin 1 (SH3 domain protein) (itsn1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005170972.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCTCAGT TTCCCACACC CTTCGGAGGG GGGTCGGATA CATGGGTAAT ATCTGTGGAC 
    GAGAGAGCCA AACATGACCA GCAGTTTCAC AGTCTCACCC CAACACCCGC TGGCTTCATC
    ACAGGTGACC AGGCAAGGAA TTTCTTCCTG CAGTCAGGTT TACCTGCCCC CATCTTGGCC
    CAGATATGGG CTCTGGCCGA CATGAACAAT GACGGGAAGA TGGACATGCA TGAGTTTTCC
    ATCGCAATGA AGCTGATTAA ATTAAAGCTG CAGGGTCACC CTCTTCCTCC ATCACTGCCC
    CCCTCCATGA AGCAACAGCC CATCTCCATA CCCCCGCCGA CCCCTTTTGG GATACCCTCT
    ATGGGTGCAA TGCCAGGCCT GCCAGCAGTG CCGCCCATGC CTCTGCCCCC TCTGCCTCCA
    GGAGTGCCAG GGATGGGTGT TTCTCCTCCA CTGGGCGCAT CGGTAACCCC ACCCGTACCA
    TCCCTCGCCA ACGGAGCACC CGCAATGATA CAACCCCTGA GTGGATTTTC ACATCCAGGT
    GCCGTGCTCA ACAAAACCCC CTCATTCAAT CGCTCCAGTA TCAAGCTACA GAAGGGCCAG
    TCTGTAGAGG CGCCCAGTGC TCCAGCTGCT CCTCCACCCG CTGACTGGGC CGTGTCTCAG
    TCCTCCAGAC TTAAGTATCG CCAGCTCTTC AACAGCCATG ATAAAATGAT GAGCGGTTAT
    CTCACAGGTC CACAAGCCAG AACTATTCTG ATGCAGTCCA GTCTACCACA AGCCCAGCTG
    GCCACCATCT GGAATCTGTC AGATATAGAT CAGGATGGGA AGTTGACCGC TGAAGAGTTT
    ATTTTGGCCA TGCACTTGAT CGACATGGCC ATGTCTGGTC TCCCTCTTCC CCCTTTGCTG
    CCGCCTGACC TTATACCTCC AACATTCAGG AGAATGCGCT CTGGTAGTGG AGTGTCTGTG
    ACTAGCATGC ACTCGACAGA CTTGCGGTCT CAGGAAGAGC CAGAGGAGGA GGAGAAAGAG
    GCCGAGAAAA AACTTCCAGT CACATTTGAG GATAAGAAAC GAGAGAACTT TGAGCGCGGA
    AACCTGGAGT TGGAGAAACG TCGACAGGCA CTTCTGGAGC AGCAGAGGAA AGAGCAGGAG
    CGACTGGCGG CGCTGGAGAA AGAAGAGCAG GAGCGGAAGG AGAGGGAACG ACTGGAGCAG
    GAGAGACGCA GACAGCAGGA GCTGGACAAA CAGCTGGAGA GACAGAGGGA GCTGGAGCGA
    CAGCGGGAGG AGGAGAGACG CAAGGAGATC GAGAGAAGAG AGGCTGCTAA ACGGGAGCTG
    GAGCGACAGA GGCAGCTGGA GTGGGAGAGG ACGCGCAGAC AGGAGCTGCT CACCCAAAGG
    AACAGAGAAC AGGAGAACAT CGTCCTGCTC AAAGCACGGA AGAAAACTCT CGAGTTTGAG
    CTGGAGGCTC TGAATGATAA GAAGTCTCAG TTGGAGGGGA AACTGCAGGA TATTCGGTCC
    CGTCTCTCGA TTCAGAGGCA TGAGATCGAG ACCACCAATA AGACTCGGGA GCTCCGCATT
    GCAGAGATCA CAAGCCTGCA ACAGCAGCTG CAGGAGTCTC AGCAGTGGTT GAGTCGCTTG
    ATTCCTGATA AACAGTGTCT GAATGACCAG CTGAAGCAGG TTCAGCAGAA CAGTCTGCAC
    AGAGACTCTT TGAACAGTCT TCAGAGGGCC ATTGAAATGA AAGAATCCAC CAAACAGCAG
    CTGAAAGAAC AGCTGGATGC TGTGGAGAAG GAAACTCGCT CCAAACTATT GGAGATCGAC
    GCCTTCAACA CACAGCTGAA GGAGCTGAGA GAAATCCACA ACAAACAGCA GAGGCAGAAA
    CAGAAGGAGC TGGAGGCTGA CATCACCCAG CCGTCACATG ACAGGAAGTC CATAGACTCA
    CAAGACTCGA GGTTGTCGGG GACTGATGAT GGCGTGTCTC CAGCGTGGAG GGATGATGGG
    CTGGGTAAAG CTCCTACTCC TCCTGTGTCT CAGGCCTGGA TGAGCCGGGT GAGTGAAGAA
    GAAAACCACA GCCGAGCTGA AATACAGGAT AACCTCTCCA AGCTTTTCAC CCAACATCCA
    CAAGCAGGAA AAATACAGGC TCAGTCTCCC TGGTCCATGG CAGATAAGAT CCCAGTGTCT
    GGTTTTAATC AGGAGAAGGT AAAGGTGGTC TACTATAGAG CAATGTATCC CTTTGAGGCC
    CGCAGTCATG ATGAGATCAC CATCCATCCT GGAGACATAG TCATGGTGGA TGAGTCTCAG
    ACAGGTGAGC CTGGATGGTT AGGAGGAGAG ATTAAAGGGA AGACTGGCTG GTTTCCTGCC
    AACTATGCGG AGAAGATTCC AGAATCCGAA GTTCCTCTGA GTTTGAGGGC CAGCGCTGCT
    TCTAGCTCCG CCCCTAAATT GGGTTCTCAC ATGTCATCTG CTTCTTCATC GACCACGGCC
    ACGCCCATTC TGCCAGTCTC CACAGAGCCT GCATCGATAG CCCCGCCCTC TTCAGCCCCA
    CCCCCCACAG GCCCCGCCTC CTCCTCTTCC TCAGCATCAA GCAATTGGGC AGATTTCAGC
    ACTACATGGC CCTCTAATTC TGCTGTGGAG AAGCAGGATA GTGATGGATG GGATGCATGG
    CCGACTCAGC CAACTCAGCC TTCTCTGTCA GTGCCCAGTG GAGGGCAGAT CAGACAGCGC
    TCTGCTTTCA CGCCTGCTAC ACTCTCCGGC TCCTCCCCCT CACCCGTGCT TGGACAGGGT
    GAGAAGGTGG AGGGTCTGCA GGCTCAGGCG CTGTACCCAT GGAGAGCCAA GAAAGACAAT
    CATCTAAACT TCAACAAGAA TGACGTGATC ACTGTACTGG AGCAGCAGGA CATGTGGTGG
    TTTGGGGAGG TGCAAGGACA GAGAGGATGG TTCCCTAAAT CTTACGTCAA GCTCATCTCT
    GGACCTGTCC GGAAATCTAT GAGTATTGAG TCTGGCTCTT CAGACAGCCC TCCTAGTGTA
    AAGAGACCCA GTCCCTCTCT AAACAAACCC ACAGAGCTCG GAGAAGGTCA GAACTCCAAC
    AGCAACTCAA TGTATCCGTC TAAAGAGTAT GTGGCCATGT ATACATATGA AAGCAATGAG
    CAGGGTGACT TGACCTTCCA GCAAGGTGAT GTCATCACAG TCACCAAGAA AGAAGGAGAT
    TGGTGGACCG GCACTGTGAG CGGGAAGATC GGAGTCTTTC CATCGAATTA TGTCAAACCT
    AAAGAATCTG AGGGGTTGGG ATCTGCTGGG AAAACAGGAA GTTTAGGGAA GAAACCAGAG
    ATTGCCCAAG TCATCGCTCC ATACACGGCC ACTGGTGCAG AGCAGCTCAC TTTAGCACCA
    GGCCAGCTCA TTCTCATTCG CAAGAAAAAC CCGGGAGGAT GGTGGGAAGG AGAACTTCAG
    GCAAGAGGAA AGAAGCGGCA GATTGGCTGG TTTCCTGCAA ATTATGTCAA ATTATTGAGT
    CCCAGTACCA GTAAGACAAC ACCCACAGAT CCCAACCCAC CCAAGCTGCC GACACCCAAT
    GCAGTGTGCC AGGTGATTGG CATGTATGAT TACACTGCTC AGAATGATGA CGAGCTGCCT
    TTCGGGAAGG GCCAAATCAT CAATGTGTTG AGCAGAGAGG ATCCCGATTG GTGGAAAGGA
    GAGTTGAATG GATCCGTCGG CCTCTTCCCC TCGAACTACG TCAAGCTGAC CACCGACACA
    GACCCCAGCC AACAATGGTG TGCTGATCTC CACCTGCTCG ACATGCTGAC CCCAGTGGAG
    CGGAAGAGGC AGGGATACAT ACATGAGCTT ATTGTTACAG AGGAGAACTA TGTCAATGAC
    CTGCAGCTGG TCACTGAGAC CTTCCAGAAG CCTCTGCTGG AGTCTGATTT GTTGACGGAG
    AAGGAGGTTG CCATGATCTT TGTCAACTGG AAAGAGCTGA CCATGTGCAA CATTAAACTG
    CTCAAGGCGC TGCGGGTGAG GAAGAAGATG TCAGGTGAGC GGATGCCAGT GAAGATGATT
    GGTGACATCC TGACGGCTCA GCTTCCTCAC ATGCAGCCAT ACATCCGCTT CTGCAGCTGC
    CAGCTCAACG GCGCCACCCT CATTCAGCAG AAAACTGACG AGGTGCCCGA ATTCAAGGAC
    TTTGTGAAGA GGTTAGCGAT GGACCCTCGC TGTAAGGGAA TGCCCCTGTC CAGTTTCCTT
    CTCAAACCCA TGCAGAGAGT CACCCGATAC CCCCTCATCA TCAAAAACAT TTTAGAGAAC
    ACTCCAGAAA GCCACCCGGA CCACAGTCAC CTGCGTCTGG CTCTGGAGAA AGCAGAAGAG
    CTGTGCTCGC AGGTCAATGA AGGTGTACGA GAGAAAGAAA ACTCCGACCG GCTGGAATGG
    ATACAGGCAC ATGTGCAGTG TGAGGGACTG TCAGAGCAAC TTGTGTTCAA CTCTGTAACA
    AACTGTCTGG GCCCTCGGAA GTTCCTGCAC AGTGGAAAAC TCTACAAAGC CAAGAGCAAC
    AAAGAGCTTT ACGGCTTCCT TTTTAACGAC TTCCTGCTCC TCACACAGAT AATCAAACCT
    CTGGGCTCAT CCGGGACAGA CAAGGTCTTC AGTGCGAAAT CCCATCTGCA GTATCGCATG
    TACAAAACGC CCATCTTTCT CAATGAGGTG CTAGTGAAGC TGCCTACTGA CCCCTCGGGA
    GATGAACCCA TTTTTCACAT ATCTCACATT GACAGAGTCT ACACTATACG GGCTGAGAGC
    ATCAACGAAA GGACAGCTTG GGTGCAGAAA ATCAAAGCTG CCTCTGAGCT CTTCATCGAA
    ACCGAAAAGA GGAAGAGAGA GAAAGCCTAC TTGGTGAGGT CTCAGAGGGC CACTGGCATC
    GGGCGGCTAA TGGTCAACAT TGTTGAAGGC ATCGAACTGA AGCCCTGCCG CTCAAATGGT
    AAGAGTAACC CGTACTGTGA GGTGACGATG GGCTCTCAGT GTCACGTCAC CAAGACCCTT
    CAGGACACTC TGAACCCCAA GTGGAACTCA AACTGCCAGT TCTTCATCAA AGACCTGGAG
    CAGGACGTGC TGTGTGTCAC TGTGTTTGAG AGGGATCAGT TCTCCCCGGA CGATTTCTTG
    GGCAGGACGG AGATTCGGCT GGCCGACATC AAGAAGGACC AGGGCTCGAA GGGTCCCATC
    ACCAAGCGTC TGCTTTTACA CGAAGTCCCG ACGGGAGAGA TTGTGGTGCG ACTGGACCTG
    CAGCTCTTCG ATGAACCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa06109
    Clone ID Related Accession (Same CDS sequence) XM_005170974.4 , XM_005170974.3
    Accession Version XM_005170974.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3753bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product intersectin-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005170974.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCTCAGT TTCCCACACC CTTCGGAGGG GGGTCGGATA CATGGGTAAT ATCTGTGGAC 
    GAGAGAGCCA AACATGACCA GCAGTTTCAC AGTCTCACCC CAACACCCGC TGGCTTCATC
    ACAGGTGACC AGGCAAGGAA TTTCTTCCTG CAGTCAGGTT TACCTGCCCC CATCTTGGCC
    CAGATATGGG CTCTGGCCGA CATGAACAAT GACGGGAAGA TGGACATGCA TGAGTTTTCC
    ATCGCAATGA AGCTGATTAA ATTAAAGCTG CAGGGTCACC CTCTTCCTCC ATCACTGCCC
    CCCTCCATGA AGCAACAGCC CATCTCCATA CCCCCGCCGA CCCCTTTTGG GATACCCTCT
    ATGGGTGCAA TGCCAGGCCT GCCAGCAGTG CCGCCCATGC CTCTGCCCCC TCTGCCTCCA
    GGAGTGCCAG GGATGGGTGT TTCTCCTCCA CTGGGCGCAT CGGTAACCCC ACCCGTACCA
    TCCCTCGCCA ACGGAGCACC CGCAATGATA CAACCCCTGA GTGGATTTTC ACATCCAGGT
    GCCGTGCTCA ACAAAACCCC CTCATTCAAT CGCTCCAGTA TCAAGCTACA GAAGGGCCAG
    TCTGTAGAGG CGCCCAGTGC TCCAGCTGCT CCTCCACCCG CTGACTGGGC CGTGTCTCAG
    TCCTCCAGAC TTAAGTATCG CCAGCTCTTC AACAGCCATG ATAAAATGAT GAGCGGTTAT
    CTCACAGGTC CACAAGCCAG AACTATTCTG ATGCAGTCCA GTCTACCACA AGCCCAGCTG
    GCCACCATCT GGAATCTGTC AGATATAGAT CAGGATGGGA AGTTGACCGC TGAAGAGTTT
    ATTTTGGCCA TGCACTTGAT CGACATGGCC ATGTCTGGTC TCCCTCTTCC CCCTTTGCTG
    CCGCCTGACC TTATACCTCC AACATTCAGG AGAATGCGCT CTGGTAGTGG AGTGTCTGTG
    ACTAGCATGC ACTCGACAGA CTTGCGGTCT CAGGAAGAGC CAGAGGAGGA GGAGAAAGAG
    GCCGAGAAAA AACTTCCAGT CACATTTGAG GATAAGAAAC GAGAGAACTT TGAGCGCGGA
    AACCTGGAGT TGGAGAAACG TCGACAGGCA CTTCTGGAGC AGCAGAGGAA AGAGCAGGAG
    CGACTGGCGG CGCTGGAGAA AGAAGAGCAG GAGCGGAAGG AGAGGGAACG ACTGGAGCAG
    GAGAGACGCA GACAGCAGGA GCTGGACAAA CAGCTGGAGA GACAGAGGGA GCTGGAGCGA
    CAGCGGGAGG AGGAGAGACG CAAGGAGATC GAGAGAAGAG AGGCTGCTAA ACGGGAGCTG
    GAGCGACAGA GGCAGCTGGA GTGGGAGAGG ACGCGCAGAC AGGAGCTGCT CACCCAAAGG
    AACAGAGAAC AGGAGAACAT CGTCCTGCTC AAAGCACGGA AGAAAACTCT CGAGTTTGAG
    CTGGAGGCTC TGAATGATAA GAAGTCTCAG TTGGAGGGGA AACTGCAGGA TATTCGGTCC
    CGTCTCTCGA TTCAGAGGCA TGAGATCGAG ACCACCAATA AGACTCGGGA GCTCCGCATT
    GCAGAGATCA CAAGCCTGCA ACAGCAGCTG CAGGAGTCTC AGCAGTGGTT GAGTCGCTTG
    ATTCCTGATA AACAGTGTCT GAATGACCAG CTGAAGCAGG TTCAGCAGAA CAGTCTGCAC
    AGAGACTCTT TGAACAGTCT TCAGAGGGCC ATTGAAATGA AAGAATCCAC CAAACAGCAG
    CTGAAAGAAC AGCTGGATGC TGTGGAGAAG GAAACTCGCT CCAAACTATT GGAGATCGAC
    GCCTTCAACA CACAGCTGAA GGAGCTGAGA GAAATCCACA ACAAACAGCA GAGGCAGAAA
    CAGAAGGAGC TGGAGGCTGA CATCACCCAG CCGTCACATG ACAGGAAGTC CATAGACTCA
    CAAGACTCGA GGTTGTCGGG GACTGATGAT GGCGTGTCTC CAGCGTGGAG GGATGATGGG
    CTGGGTAAAG CTCCTACTCC TCCTGTGTCT CAGGCCTGGA TGAGCCGGGT GAGTGAAGAA
    GAAAACCACA GCCGAGCTGA AATACAGGAT AACCTCTCCA AGCTTTTCAC CCAACATCCA
    CAAGCAGGAA AAATACAGGC TCAGTCTCCC TGGTCCATGG CAGATAAGAT CCCAGTGTCT
    GGTTTTAATC AGGAGAAGGT AAAGGTGGTC TACTATAGAG CAATGTATCC CTTTGAGGCC
    CGCAGTCATG ATGAGATCAC CATCCATCCT GGAGACATAG TCATGGTGAA GGGAGAGTGG
    GTGGATGAGT CTCAGACAGG TGAGCCTGGA TGGTTAGGAG GAGAGATTAA AGGGAAGACT
    GGCTGGTTTC CTGCCAACTA TGCGGAGAAG ATTCCAGAAT CCGAAGTTCC TCTGAGTTTG
    AGGGCCAGCG CTGCTTCTAG CTCCGCCCCT AAATTGGGTT CTCACATGTC ATCTGCTTCT
    TCATCGACCA CGGCCACGCC CATTCTGCCA GTCTCCACAG AGCCTGCATC GATAGCCCCG
    CCCTCTTCAG CCCCACCCCC CACAGGCCCC GCCTCCTCCT CTTCCTCAGC ATCAAGCAAT
    TGGGCAGATT TCAGCACTAC ATGGCCCTCT AATTCTGCTG TGGAGAAGCA GGATAGTGAT
    GGATGGGATG CATGGCCGAC TCAGCCAACT CAGCCTTCTC TGTCAGTGCC CAGTGGAGGG
    CAGATCAGAC AGCGCTCTGC TTTCACGCCT GCTACACTCT CCGGCTCCTC CCCCTCACCC
    GTGCTTGGAC AGGGTGAGAA GGTGGAGGGT CTGCAGGCTC AGGCGCTGTA CCCATGGAGA
    GCCAAGAAAG ACAATCATCT AAACTTCAAC AAGAATGACG TGATCACTGT ACTGGAGCAG
    CAGGACATGT GGTGGTTTGG GGAGGTGCAA GGACAGAGAG GATGGTTCCC TAAATCTTAC
    GTCAAGCTCA TCTCTGGACC TGTCCGGAAA TCTATGAGTA TTGAGTCTGG CTCTTCAGAC
    AGCCCTCCTA GTGTAAAGAG ACCCAGTCCC TCTCTAAACA AACCCACAGA GCTCGGAGAA
    GGTCAGAACT CCAACAGCAA CTCAATGTAT CCGTCTAAAG AGTATGTGGC CATGTATACA
    TATGAAAGCA ATGAGCAGGG TGACTTGACC TTCCAGCAAG GTGATGTCAT CACAGTCACC
    AAGAAAGAAG GAGATTGGTG GACCGGCACT GTGAGCGGGA AGATCGGAGT CTTTCCATCG
    AATTATGTCA AACCTAAAGA ATCTGAGGGG TTGGGATCTG CTGGGAAAAC AGGAAGTTTA
    GGGAAGAAAC CAGAGATTGC CCAAGTCATC GCTCCATACA CGGCCACTGG TGCAGAGCAG
    CTCACTTTAG CACCAGGCCA GCTCATTCTC ATTCGCAAGA AAAACCCGGG AGGATGGTGG
    GAAGGAGAAC TTCAGGCAAG AGGAAAGAAG CGGCAGATTG GCTGGTTTCC TGCAAATTAT
    GTCAAATTAT TGAGTCCCAG TACCAGTAAG ACAACACCCA CAGATCCCAA CCCACCCAAG
    CTGCCGACAC CCAATGCAGT GTGCCAGGTG ATTGGCATGT ATGATTACAC TGCTCAGAAT
    GATGACGAGC TGCCTTTCGG GAAGGGCCAA ATCATCAATG TGTTGAGCAG AGAGGATCCC
    GATTGGTGGA AAGGAGAGTT GAATGGATCC GTCGGCCTCT TCCCCTCGAA CTACGTCAAG
    CTGACCACCG ACACAGACCC CAGCCAACAA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005171031.1
    CDS226..3978
    Translation

    Target ORF information:

    RefSeq Version XM_005170974.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio intersectin 1 (SH3 domain protein) (itsn1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005170974.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCTCAGT TTCCCACACC CTTCGGAGGG GGGTCGGATA CATGGGTAAT ATCTGTGGAC 
    GAGAGAGCCA AACATGACCA GCAGTTTCAC AGTCTCACCC CAACACCCGC TGGCTTCATC
    ACAGGTGACC AGGCAAGGAA TTTCTTCCTG CAGTCAGGTT TACCTGCCCC CATCTTGGCC
    CAGATATGGG CTCTGGCCGA CATGAACAAT GACGGGAAGA TGGACATGCA TGAGTTTTCC
    ATCGCAATGA AGCTGATTAA ATTAAAGCTG CAGGGTCACC CTCTTCCTCC ATCACTGCCC
    CCCTCCATGA AGCAACAGCC CATCTCCATA CCCCCGCCGA CCCCTTTTGG GATACCCTCT
    ATGGGTGCAA TGCCAGGCCT GCCAGCAGTG CCGCCCATGC CTCTGCCCCC TCTGCCTCCA
    GGAGTGCCAG GGATGGGTGT TTCTCCTCCA CTGGGCGCAT CGGTAACCCC ACCCGTACCA
    TCCCTCGCCA ACGGAGCACC CGCAATGATA CAACCCCTGA GTGGATTTTC ACATCCAGGT
    GCCGTGCTCA ACAAAACCCC CTCATTCAAT CGCTCCAGTA TCAAGCTACA GAAGGGCCAG
    TCTGTAGAGG CGCCCAGTGC TCCAGCTGCT CCTCCACCCG CTGACTGGGC CGTGTCTCAG
    TCCTCCAGAC TTAAGTATCG CCAGCTCTTC AACAGCCATG ATAAAATGAT GAGCGGTTAT
    CTCACAGGTC CACAAGCCAG AACTATTCTG ATGCAGTCCA GTCTACCACA AGCCCAGCTG
    GCCACCATCT GGAATCTGTC AGATATAGAT CAGGATGGGA AGTTGACCGC TGAAGAGTTT
    ATTTTGGCCA TGCACTTGAT CGACATGGCC ATGTCTGGTC TCCCTCTTCC CCCTTTGCTG
    CCGCCTGACC TTATACCTCC AACATTCAGG AGAATGCGCT CTGGTAGTGG AGTGTCTGTG
    ACTAGCATGC ACTCGACAGA CTTGCGGTCT CAGGAAGAGC CAGAGGAGGA GGAGAAAGAG
    GCCGAGAAAA AACTTCCAGT CACATTTGAG GATAAGAAAC GAGAGAACTT TGAGCGCGGA
    AACCTGGAGT TGGAGAAACG TCGACAGGCA CTTCTGGAGC AGCAGAGGAA AGAGCAGGAG
    CGACTGGCGG CGCTGGAGAA AGAAGAGCAG GAGCGGAAGG AGAGGGAACG ACTGGAGCAG
    GAGAGACGCA GACAGCAGGA GCTGGACAAA CAGCTGGAGA GACAGAGGGA GCTGGAGCGA
    CAGCGGGAGG AGGAGAGACG CAAGGAGATC GAGAGAAGAG AGGCTGCTAA ACGGGAGCTG
    GAGCGACAGA GGCAGCTGGA GTGGGAGAGG ACGCGCAGAC AGGAGCTGCT CACCCAAAGG
    AACAGAGAAC AGGAGAACAT CGTCCTGCTC AAAGCACGGA AGAAAACTCT CGAGTTTGAG
    CTGGAGGCTC TGAATGATAA GAAGTCTCAG TTGGAGGGGA AACTGCAGGA TATTCGGTCC
    CGTCTCTCGA TTCAGAGGCA TGAGATCGAG ACCACCAATA AGACTCGGGA GCTCCGCATT
    GCAGAGATCA CAAGCCTGCA ACAGCAGCTG CAGGAGTCTC AGCAGTGGTT GAGTCGCTTG
    ATTCCTGATA AACAGTGTCT GAATGACCAG CTGAAGCAGG TTCAGCAGAA CAGTCTGCAC
    AGAGACTCTT TGAACAGTCT TCAGAGGGCC ATTGAAATGA AAGAATCCAC CAAACAGCAG
    CTGAAAGAAC AGCTGGATGC TGTGGAGAAG GAAACTCGCT CCAAACTATT GGAGATCGAC
    GCCTTCAACA CACAGCTGAA GGAGCTGAGA GAAATCCACA ACAAACAGCA GAGGCAGAAA
    CAGAAGGAGC TGGAGGCTGA CATCACCCAG CCGTCACATG ACAGGAAGTC CATAGACTCA
    CAAGACTCGA GGTTGTCGGG GACTGATGAT GGCGTGTCTC CAGCGTGGAG GGATGATGGG
    CTGGGTAAAG CTCCTACTCC TCCTGTGTCT CAGGCCTGGA TGAGCCGGGT GAGTGAAGAA
    GAAAACCACA GCCGAGCTGA AATACAGGAT AACCTCTCCA AGCTTTTCAC CCAACATCCA
    CAAGCAGGAA AAATACAGGC TCAGTCTCCC TGGTCCATGG CAGATAAGAT CCCAGTGTCT
    GGTTTTAATC AGGAGAAGGT AAAGGTGGTC TACTATAGAG CAATGTATCC CTTTGAGGCC
    CGCAGTCATG ATGAGATCAC CATCCATCCT GGAGACATAG TCATGGTGAA GGGAGAGTGG
    GTGGATGAGT CTCAGACAGG TGAGCCTGGA TGGTTAGGAG GAGAGATTAA AGGGAAGACT
    GGCTGGTTTC CTGCCAACTA TGCGGAGAAG ATTCCAGAAT CCGAAGTTCC TCTGAGTTTG
    AGGGCCAGCG CTGCTTCTAG CTCCGCCCCT AAATTGGGTT CTCACATGTC ATCTGCTTCT
    TCATCGACCA CGGCCACGCC CATTCTGCCA GTCTCCACAG AGCCTGCATC GATAGCCCCG
    CCCTCTTCAG CCCCACCCCC CACAGGCCCC GCCTCCTCCT CTTCCTCAGC ATCAAGCAAT
    TGGGCAGATT TCAGCACTAC ATGGCCCTCT AATTCTGCTG TGGAGAAGCA GGATAGTGAT
    GGATGGGATG CATGGCCGAC TCAGCCAACT CAGCCTTCTC TGTCAGTGCC CAGTGGAGGG
    CAGATCAGAC AGCGCTCTGC TTTCACGCCT GCTACACTCT CCGGCTCCTC CCCCTCACCC
    GTGCTTGGAC AGGGTGAGAA GGTGGAGGGT CTGCAGGCTC AGGCGCTGTA CCCATGGAGA
    GCCAAGAAAG ACAATCATCT AAACTTCAAC AAGAATGACG TGATCACTGT ACTGGAGCAG
    CAGGACATGT GGTGGTTTGG GGAGGTGCAA GGACAGAGAG GATGGTTCCC TAAATCTTAC
    GTCAAGCTCA TCTCTGGACC TGTCCGGAAA TCTATGAGTA TTGAGTCTGG CTCTTCAGAC
    AGCCCTCCTA GTGTAAAGAG ACCCAGTCCC TCTCTAAACA AACCCACAGA GCTCGGAGAA
    GGTCAGAACT CCAACAGCAA CTCAATGTAT CCGTCTAAAG AGTATGTGGC CATGTATACA
    TATGAAAGCA ATGAGCAGGG TGACTTGACC TTCCAGCAAG GTGATGTCAT CACAGTCACC
    AAGAAAGAAG GAGATTGGTG GACCGGCACT GTGAGCGGGA AGATCGGAGT CTTTCCATCG
    AATTATGTCA AACCTAAAGA ATCTGAGGGG TTGGGATCTG CTGGGAAAAC AGGAAGTTTA
    GGGAAGAAAC CAGAGATTGC CCAAGTCATC GCTCCATACA CGGCCACTGG TGCAGAGCAG
    CTCACTTTAG CACCAGGCCA GCTCATTCTC ATTCGCAAGA AAAACCCGGG AGGATGGTGG
    GAAGGAGAAC TTCAGGCAAG AGGAAAGAAG CGGCAGATTG GCTGGTTTCC TGCAAATTAT
    GTCAAATTAT TGAGTCCCAG TACCAGTAAG ACAACACCCA CAGATCCCAA CCCACCCAAG
    CTGCCGACAC CCAATGCAGT GTGCCAGGTG ATTGGCATGT ATGATTACAC TGCTCAGAAT
    GATGACGAGC TGCCTTTCGG GAAGGGCCAA ATCATCAATG TGTTGAGCAG AGAGGATCCC
    GATTGGTGGA AAGGAGAGTT GAATGGATCC GTCGGCCTCT TCCCCTCGAA CTACGTCAAG
    CTGACCACCG ACACAGACCC CAGCCAACAA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa06109
    Clone ID Related Accession (Same CDS sequence) XM_005170974.4 , XM_005170974.3
    Accession Version XM_005170974.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3753bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product intersectin-1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005170974.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCTCAGT TTCCCACACC CTTCGGAGGG GGGTCGGATA CATGGGTAAT ATCTGTGGAC 
    GAGAGAGCCA AACATGACCA GCAGTTTCAC AGTCTCACCC CAACACCCGC TGGCTTCATC
    ACAGGTGACC AGGCAAGGAA TTTCTTCCTG CAGTCAGGTT TACCTGCCCC CATCTTGGCC
    CAGATATGGG CTCTGGCCGA CATGAACAAT GACGGGAAGA TGGACATGCA TGAGTTTTCC
    ATCGCAATGA AGCTGATTAA ATTAAAGCTG CAGGGTCACC CTCTTCCTCC ATCACTGCCC
    CCCTCCATGA AGCAACAGCC CATCTCCATA CCCCCGCCGA CCCCTTTTGG GATACCCTCT
    ATGGGTGCAA TGCCAGGCCT GCCAGCAGTG CCGCCCATGC CTCTGCCCCC TCTGCCTCCA
    GGAGTGCCAG GGATGGGTGT TTCTCCTCCA CTGGGCGCAT CGGTAACCCC ACCCGTACCA
    TCCCTCGCCA ACGGAGCACC CGCAATGATA CAACCCCTGA GTGGATTTTC ACATCCAGGT
    GCCGTGCTCA ACAAAACCCC CTCATTCAAT CGCTCCAGTA TCAAGCTACA GAAGGGCCAG
    TCTGTAGAGG CGCCCAGTGC TCCAGCTGCT CCTCCACCCG CTGACTGGGC CGTGTCTCAG
    TCCTCCAGAC TTAAGTATCG CCAGCTCTTC AACAGCCATG ATAAAATGAT GAGCGGTTAT
    CTCACAGGTC CACAAGCCAG AACTATTCTG ATGCAGTCCA GTCTACCACA AGCCCAGCTG
    GCCACCATCT GGAATCTGTC AGATATAGAT CAGGATGGGA AGTTGACCGC TGAAGAGTTT
    ATTTTGGCCA TGCACTTGAT CGACATGGCC ATGTCTGGTC TCCCTCTTCC CCCTTTGCTG
    CCGCCTGACC TTATACCTCC AACATTCAGG AGAATGCGCT CTGGTAGTGG AGTGTCTGTG
    ACTAGCATGC ACTCGACAGA CTTGCGGTCT CAGGAAGAGC CAGAGGAGGA GGAGAAAGAG
    GCCGAGAAAA AACTTCCAGT CACATTTGAG GATAAGAAAC GAGAGAACTT TGAGCGCGGA
    AACCTGGAGT TGGAGAAACG TCGACAGGCA CTTCTGGAGC AGCAGAGGAA AGAGCAGGAG
    CGACTGGCGG CGCTGGAGAA AGAAGAGCAG GAGCGGAAGG AGAGGGAACG ACTGGAGCAG
    GAGAGACGCA GACAGCAGGA GCTGGACAAA CAGCTGGAGA GACAGAGGGA GCTGGAGCGA
    CAGCGGGAGG AGGAGAGACG CAAGGAGATC GAGAGAAGAG AGGCTGCTAA ACGGGAGCTG
    GAGCGACAGA GGCAGCTGGA GTGGGAGAGG ACGCGCAGAC AGGAGCTGCT CACCCAAAGG
    AACAGAGAAC AGGAGAACAT CGTCCTGCTC AAAGCACGGA AGAAAACTCT CGAGTTTGAG
    CTGGAGGCTC TGAATGATAA GAAGTCTCAG TTGGAGGGGA AACTGCAGGA TATTCGGTCC
    CGTCTCTCGA TTCAGAGGCA TGAGATCGAG ACCACCAATA AGACTCGGGA GCTCCGCATT
    GCAGAGATCA CAAGCCTGCA ACAGCAGCTG CAGGAGTCTC AGCAGTGGTT GAGTCGCTTG
    ATTCCTGATA AACAGTGTCT GAATGACCAG CTGAAGCAGG TTCAGCAGAA CAGTCTGCAC
    AGAGACTCTT TGAACAGTCT TCAGAGGGCC ATTGAAATGA AAGAATCCAC CAAACAGCAG
    CTGAAAGAAC AGCTGGATGC TGTGGAGAAG GAAACTCGCT CCAAACTATT GGAGATCGAC
    GCCTTCAACA CACAGCTGAA GGAGCTGAGA GAAATCCACA ACAAACAGCA GAGGCAGAAA
    CAGAAGGAGC TGGAGGCTGA CATCACCCAG CCGTCACATG ACAGGAAGTC CATAGACTCA
    CAAGACTCGA GGTTGTCGGG GACTGATGAT GGCGTGTCTC CAGCGTGGAG GGATGATGGG
    CTGGGTAAAG CTCCTACTCC TCCTGTGTCT CAGGCCTGGA TGAGCCGGGT GAGTGAAGAA
    GAAAACCACA GCCGAGCTGA AATACAGGAT AACCTCTCCA AGCTTTTCAC CCAACATCCA
    CAAGCAGGAA AAATACAGGC TCAGTCTCCC TGGTCCATGG CAGATAAGAT CCCAGTGTCT
    GGTTTTAATC AGGAGAAGGT AAAGGTGGTC TACTATAGAG CAATGTATCC CTTTGAGGCC
    CGCAGTCATG ATGAGATCAC CATCCATCCT GGAGACATAG TCATGGTGAA GGGAGAGTGG
    GTGGATGAGT CTCAGACAGG TGAGCCTGGA TGGTTAGGAG GAGAGATTAA AGGGAAGACT
    GGCTGGTTTC CTGCCAACTA TGCGGAGAAG ATTCCAGAAT CCGAAGTTCC TCTGAGTTTG
    AGGGCCAGCG CTGCTTCTAG CTCCGCCCCT AAATTGGGTT CTCACATGTC ATCTGCTTCT
    TCATCGACCA CGGCCACGCC CATTCTGCCA GTCTCCACAG AGCCTGCATC GATAGCCCCG
    CCCTCTTCAG CCCCACCCCC CACAGGCCCC GCCTCCTCCT CTTCCTCAGC ATCAAGCAAT
    TGGGCAGATT TCAGCACTAC ATGGCCCTCT AATTCTGCTG TGGAGAAGCA GGATAGTGAT
    GGATGGGATG CATGGCCGAC TCAGCCAACT CAGCCTTCTC TGTCAGTGCC CAGTGGAGGG
    CAGATCAGAC AGCGCTCTGC TTTCACGCCT GCTACACTCT CCGGCTCCTC CCCCTCACCC
    GTGCTTGGAC AGGGTGAGAA GGTGGAGGGT CTGCAGGCTC AGGCGCTGTA CCCATGGAGA
    GCCAAGAAAG ACAATCATCT AAACTTCAAC AAGAATGACG TGATCACTGT ACTGGAGCAG
    CAGGACATGT GGTGGTTTGG GGAGGTGCAA GGACAGAGAG GATGGTTCCC TAAATCTTAC
    GTCAAGCTCA TCTCTGGACC TGTCCGGAAA TCTATGAGTA TTGAGTCTGG CTCTTCAGAC
    AGCCCTCCTA GTGTAAAGAG ACCCAGTCCC TCTCTAAACA AACCCACAGA GCTCGGAGAA
    GGTCAGAACT CCAACAGCAA CTCAATGTAT CCGTCTAAAG AGTATGTGGC CATGTATACA
    TATGAAAGCA ATGAGCAGGG TGACTTGACC TTCCAGCAAG GTGATGTCAT CACAGTCACC
    AAGAAAGAAG GAGATTGGTG GACCGGCACT GTGAGCGGGA AGATCGGAGT CTTTCCATCG
    AATTATGTCA AACCTAAAGA ATCTGAGGGG TTGGGATCTG CTGGGAAAAC AGGAAGTTTA
    GGGAAGAAAC CAGAGATTGC CCAAGTCATC GCTCCATACA CGGCCACTGG TGCAGAGCAG
    CTCACTTTAG CACCAGGCCA GCTCATTCTC ATTCGCAAGA AAAACCCGGG AGGATGGTGG
    GAAGGAGAAC TTCAGGCAAG AGGAAAGAAG CGGCAGATTG GCTGGTTTCC TGCAAATTAT
    GTCAAATTAT TGAGTCCCAG TACCAGTAAG ACAACACCCA CAGATCCCAA CCCACCCAAG
    CTGCCGACAC CCAATGCAGT GTGCCAGGTG ATTGGCATGT ATGATTACAC TGCTCAGAAT
    GATGACGAGC TGCCTTTCGG GAAGGGCCAA ATCATCAATG TGTTGAGCAG AGAGGATCCC
    GATTGGTGGA AAGGAGAGTT GAATGGATCC GTCGGCCTCT TCCCCTCGAA CTACGTCAAG
    CTGACCACCG ACACAGACCC CAGCCAACAA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005171031.1
    CDS123..3875
    Translation

    Target ORF information:

    RefSeq Version XM_005170974.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio intersectin 1 (SH3 domain protein) (itsn1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005170974.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCTCAGT TTCCCACACC CTTCGGAGGG GGGTCGGATA CATGGGTAAT ATCTGTGGAC 
    GAGAGAGCCA AACATGACCA GCAGTTTCAC AGTCTCACCC CAACACCCGC TGGCTTCATC
    ACAGGTGACC AGGCAAGGAA TTTCTTCCTG CAGTCAGGTT TACCTGCCCC CATCTTGGCC
    CAGATATGGG CTCTGGCCGA CATGAACAAT GACGGGAAGA TGGACATGCA TGAGTTTTCC
    ATCGCAATGA AGCTGATTAA ATTAAAGCTG CAGGGTCACC CTCTTCCTCC ATCACTGCCC
    CCCTCCATGA AGCAACAGCC CATCTCCATA CCCCCGCCGA CCCCTTTTGG GATACCCTCT
    ATGGGTGCAA TGCCAGGCCT GCCAGCAGTG CCGCCCATGC CTCTGCCCCC TCTGCCTCCA
    GGAGTGCCAG GGATGGGTGT TTCTCCTCCA CTGGGCGCAT CGGTAACCCC ACCCGTACCA
    TCCCTCGCCA ACGGAGCACC CGCAATGATA CAACCCCTGA GTGGATTTTC ACATCCAGGT
    GCCGTGCTCA ACAAAACCCC CTCATTCAAT CGCTCCAGTA TCAAGCTACA GAAGGGCCAG
    TCTGTAGAGG CGCCCAGTGC TCCAGCTGCT CCTCCACCCG CTGACTGGGC CGTGTCTCAG
    TCCTCCAGAC TTAAGTATCG CCAGCTCTTC AACAGCCATG ATAAAATGAT GAGCGGTTAT
    CTCACAGGTC CACAAGCCAG AACTATTCTG ATGCAGTCCA GTCTACCACA AGCCCAGCTG
    GCCACCATCT GGAATCTGTC AGATATAGAT CAGGATGGGA AGTTGACCGC TGAAGAGTTT
    ATTTTGGCCA TGCACTTGAT CGACATGGCC ATGTCTGGTC TCCCTCTTCC CCCTTTGCTG
    CCGCCTGACC TTATACCTCC AACATTCAGG AGAATGCGCT CTGGTAGTGG AGTGTCTGTG
    ACTAGCATGC ACTCGACAGA CTTGCGGTCT CAGGAAGAGC CAGAGGAGGA GGAGAAAGAG
    GCCGAGAAAA AACTTCCAGT CACATTTGAG GATAAGAAAC GAGAGAACTT TGAGCGCGGA
    AACCTGGAGT TGGAGAAACG TCGACAGGCA CTTCTGGAGC AGCAGAGGAA AGAGCAGGAG
    CGACTGGCGG CGCTGGAGAA AGAAGAGCAG GAGCGGAAGG AGAGGGAACG ACTGGAGCAG
    GAGAGACGCA GACAGCAGGA GCTGGACAAA CAGCTGGAGA GACAGAGGGA GCTGGAGCGA
    CAGCGGGAGG AGGAGAGACG CAAGGAGATC GAGAGAAGAG AGGCTGCTAA ACGGGAGCTG
    GAGCGACAGA GGCAGCTGGA GTGGGAGAGG ACGCGCAGAC AGGAGCTGCT CACCCAAAGG
    AACAGAGAAC AGGAGAACAT CGTCCTGCTC AAAGCACGGA AGAAAACTCT CGAGTTTGAG
    CTGGAGGCTC TGAATGATAA GAAGTCTCAG TTGGAGGGGA AACTGCAGGA TATTCGGTCC
    CGTCTCTCGA TTCAGAGGCA TGAGATCGAG ACCACCAATA AGACTCGGGA GCTCCGCATT
    GCAGAGATCA CAAGCCTGCA ACAGCAGCTG CAGGAGTCTC AGCAGTGGTT GAGTCGCTTG
    ATTCCTGATA AACAGTGTCT GAATGACCAG CTGAAGCAGG TTCAGCAGAA CAGTCTGCAC
    AGAGACTCTT TGAACAGTCT TCAGAGGGCC ATTGAAATGA AAGAATCCAC CAAACAGCAG
    CTGAAAGAAC AGCTGGATGC TGTGGAGAAG GAAACTCGCT CCAAACTATT GGAGATCGAC
    GCCTTCAACA CACAGCTGAA GGAGCTGAGA GAAATCCACA ACAAACAGCA GAGGCAGAAA
    CAGAAGGAGC TGGAGGCTGA CATCACCCAG CCGTCACATG ACAGGAAGTC CATAGACTCA
    CAAGACTCGA GGTTGTCGGG GACTGATGAT GGCGTGTCTC CAGCGTGGAG GGATGATGGG
    CTGGGTAAAG CTCCTACTCC TCCTGTGTCT CAGGCCTGGA TGAGCCGGGT GAGTGAAGAA
    GAAAACCACA GCCGAGCTGA AATACAGGAT AACCTCTCCA AGCTTTTCAC CCAACATCCA
    CAAGCAGGAA AAATACAGGC TCAGTCTCCC TGGTCCATGG CAGATAAGAT CCCAGTGTCT
    GGTTTTAATC AGGAGAAGGT AAAGGTGGTC TACTATAGAG CAATGTATCC CTTTGAGGCC
    CGCAGTCATG ATGAGATCAC CATCCATCCT GGAGACATAG TCATGGTGAA GGGAGAGTGG
    GTGGATGAGT CTCAGACAGG TGAGCCTGGA TGGTTAGGAG GAGAGATTAA AGGGAAGACT
    GGCTGGTTTC CTGCCAACTA TGCGGAGAAG ATTCCAGAAT CCGAAGTTCC TCTGAGTTTG
    AGGGCCAGCG CTGCTTCTAG CTCCGCCCCT AAATTGGGTT CTCACATGTC ATCTGCTTCT
    TCATCGACCA CGGCCACGCC CATTCTGCCA GTCTCCACAG AGCCTGCATC GATAGCCCCG
    CCCTCTTCAG CCCCACCCCC CACAGGCCCC GCCTCCTCCT CTTCCTCAGC ATCAAGCAAT
    TGGGCAGATT TCAGCACTAC ATGGCCCTCT AATTCTGCTG TGGAGAAGCA GGATAGTGAT
    GGATGGGATG CATGGCCGAC TCAGCCAACT CAGCCTTCTC TGTCAGTGCC CAGTGGAGGG
    CAGATCAGAC AGCGCTCTGC TTTCACGCCT GCTACACTCT CCGGCTCCTC CCCCTCACCC
    GTGCTTGGAC AGGGTGAGAA GGTGGAGGGT CTGCAGGCTC AGGCGCTGTA CCCATGGAGA
    GCCAAGAAAG ACAATCATCT AAACTTCAAC AAGAATGACG TGATCACTGT ACTGGAGCAG
    CAGGACATGT GGTGGTTTGG GGAGGTGCAA GGACAGAGAG GATGGTTCCC TAAATCTTAC
    GTCAAGCTCA TCTCTGGACC TGTCCGGAAA TCTATGAGTA TTGAGTCTGG CTCTTCAGAC
    AGCCCTCCTA GTGTAAAGAG ACCCAGTCCC TCTCTAAACA AACCCACAGA GCTCGGAGAA
    GGTCAGAACT CCAACAGCAA CTCAATGTAT CCGTCTAAAG AGTATGTGGC CATGTATACA
    TATGAAAGCA ATGAGCAGGG TGACTTGACC TTCCAGCAAG GTGATGTCAT CACAGTCACC
    AAGAAAGAAG GAGATTGGTG GACCGGCACT GTGAGCGGGA AGATCGGAGT CTTTCCATCG
    AATTATGTCA AACCTAAAGA ATCTGAGGGG TTGGGATCTG CTGGGAAAAC AGGAAGTTTA
    GGGAAGAAAC CAGAGATTGC CCAAGTCATC GCTCCATACA CGGCCACTGG TGCAGAGCAG
    CTCACTTTAG CACCAGGCCA GCTCATTCTC ATTCGCAAGA AAAACCCGGG AGGATGGTGG
    GAAGGAGAAC TTCAGGCAAG AGGAAAGAAG CGGCAGATTG GCTGGTTTCC TGCAAATTAT
    GTCAAATTAT TGAGTCCCAG TACCAGTAAG ACAACACCCA CAGATCCCAA CCCACCCAAG
    CTGCCGACAC CCAATGCAGT GTGCCAGGTG ATTGGCATGT ATGATTACAC TGCTCAGAAT
    GATGACGAGC TGCCTTTCGG GAAGGGCCAA ATCATCAATG TGTTGAGCAG AGAGGATCCC
    GATTGGTGGA AAGGAGAGTT GAATGGATCC GTCGGCCTCT TCCCCTCGAA CTACGTCAAG
    CTGACCACCG ACACAGACCC CAGCCAACAA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.