selenow1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	selenow1
Entrez Gene ID	352915
Full Name	selenoprotein W, 1
Synonyms	cb694,fe37b06,selW,selenow,sepw1,wu:fe37b06,wu:fk70h03,zgc:109781
General protein information	Preferred Names selenoprotein W, 1 Names selenoprotein W selenoprotein W1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)
Summary	This gene encodes a selenoprotein containing a selenocysteine (Sec) residue, which is encoded by the UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This protein belongs to the SelWTH family, which possesses a thioredoxin-like fold and a conserved CxxU (C is cysteine, U is Sec) motif, suggesting a redox function for this gene. Unlike in mammals, where this protein is highly expressed in skeletal muscle, heart and brain; it is expressed in sensory organs in zebrafish. Paralogs of this gene, sepw2a and sepw2b, are found on chromosome 3. [provided by RefSeq, Jul 2017].

mRNA and Protein(s)

mRNA	Protein	Name
NM_178287.5	NP_840072.3	selenoprotein W1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Sepw1	NP_037159.4
Pan troglodytes (chimpanzee)	SEPW1	NP_001148049.1
Gallus gallus (chicken)	SEPW1	NP_001159799.1
Danio rerio (zebrafish)	sepw1	NP_840072.3
Homo sapiens (human)	SEPW1	NP_003000.1
Bos taurus (cattle)	SEPW1	NP_001156697.1
	SEPW1	NP_001036202.1
Canis lupus familiaris (dog)	SEPW1	NP_001108484.1
Mus musculus (house mouse)	Sepw1	NP_033182.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Selenoprotein Gene Nomenclature.
Gladyshev VN, Arn?r ES, Berry MJ, Brigelius-Floh? R, Bruford EA, Burk RF, Carlson BA, Castellano S, Chavatte L, Conrad M, Copeland PR, Diamond AM, Driscoll DM, Ferreiro A, Floh? L, Green FR, Guig? R, Handy DE, Hatfield DL, Hesketh J, Hoffmann PR, Holmgren A, Hondal RJ, Howard MT, Huang K, Kim HY, Kim IY, K?hrle J, Krol A, Kryukov GV, Lee BJ, Lee BC, Lei XG, Liu Q, Lescure A, Lobanov AV, Loscalzo J, Maiorino M, Mariotti M, Sandeep Prabhu K, Rayman MP, Rozovsky S, Salinas G, Schmidt EE, Schomburg L, Schweizer U, Simonovi? M, Sunde RA, Tsuji PA, Tweedie S, Ursini F, Whanger PD, Zhang Y
The Journal of biological chemistry291(46)24036-24040(2016 Nov)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Roles of selenoprotein antioxidant protection in zebrafish, Danio rerio, subjected to dietary oxidative stress.
Betancor MB, Almaida-Pag?n PF, Sprague M, Hern?ndez A, Tocher DR
Fish physiology and biochemistry41(3)705-20(2015 Jun)

Selenium prevents downregulation of antioxidant selenoprotein genes by methylmercury.
Penglase S, Hamre K, Ellingsen S
Free radical biology & medicine7595-104(2014 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following selenow1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the selenow1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa38991	NM_178287.5 Latest version!	Danio rerio selenoprotein W, 1 (selenow1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

selenow1 ( NM_178287.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_840072.3 Danio rerio selenoprotein W, 1 (selenow1), mRNA.

Price & Availability↑

CloneID	ODa38991
Clone ID Related Accession (Same CDS sequence)	NM_178287.5
Accession Version	NM_178287.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Danio rerio(zebrafish)
Product	selenoprotein W1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EH593497.1, EH445855.1 and EH564833.1. On Feb 4, 2010 this sequence version replaced NM_178287.4. ##Evidence-Data-START## Transcript exon combination :: CN017424.1, EH593497.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGACCGTCA AAGTTCATGT CGTTTACTGC GGCGGATGAG GGTACCGGCC CAAGTTCATC 
AAACTCAAGA CGTTGCTTGA GGATGAATTC CCCAACGAAC TTGAGATTAC TGGTGAGGGC 
ACACCCTCGA CCACAGGCTG GTTGGAGGTG GAAGTAAACG GCAAGTTGGT GCACTCTAAG 
AAGAACGGAG ATGGATTCGT CGACTCCGAT TCAAAAATGC AGAAAATCGT GACTGCCATC 
GAGCAGGCCA TGGGGAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_840072.3
CDS	136..396
Translation	MTVKVHVVYCGG*GYRPKFIKLKTLLEDEFPNELEITGEGTPSTTGWLEVEVNGKLVHSKKNGDGFVDSDSKMQKIVTAIEQAMGK

Target ORF information:

RefSeq Version	NM_178287.5
Organism	Danio rerio(zebrafish)
Definition	Danio rerio selenoprotein W, 1 (selenow1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178287.5

ORF Insert Sequence:

ATGACCGTCA AAGTTCATGT CGTTTACTGC GGCGGATGAG GGTACCGGCC CAAGTTCATC 
AAACTCAAGA CGTTGCTTGA GGATGAATTC CCCAACGAAC TTGAGATTAC TGGTGAGGGC 
ACACCCTCGA CCACAGGCTG GTTGGAGGTG GAAGTAAACG GCAAGTTGGT GCACTCTAAG 
AAGAACGGAG ATGGATTCGT CGACTCCGAT TCAAAAATGC AGAAAATCGT GACTGCCATC 
GAGCAGGCCA TGGGGAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Selenoprotein Gene Nomenclature.
The Journal of biological chemistry291(46)24036-24040(2016 Nov)
Gladyshev VN,Arn?r ES,Berry MJ,Brigelius-Floh? R,Bruford EA,Burk RF,Carlson BA,Castellano S,Chavatte L,Conrad M,Copeland PR,Diamond AM,Driscoll DM,Ferreiro A,Floh? L,Green FR,Guig? R,Handy DE,Hatfield DL,Hesketh J,Hoffmann PR,Holmgren A,Hondal RJ,Howard MT,Huang K,Kim HY,Kim IY,K?hrle J,Krol A,Kryukov GV,Lee BJ,Lee BC,Lei XG,Liu Q,Lescure A,Lobanov AV,Loscalzo J,Maiorino M,Mariotti M,Sandeep Prabhu K,Rayman MP,Rozovsky S,Salinas G,Schmidt EE,Schomburg L,Schweizer U,Simonovi? M,Sunde RA,Tsuji PA,Tweedie S,Ursini F,Whanger PD,Zhang Y

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Roles of selenoprotein antioxidant protection in zebrafish, Danio rerio, subjected to dietary oxidative stress.
Fish physiology and biochemistry41(3)705-20(2015 Jun)
Betancor MB,Almaida-Pag?n PF,Sprague M,Hern?ndez A,Tocher DR

Selenium prevents downregulation of antioxidant selenoprotein genes by methylmercury.
Free radical biology & medicine7595-104(2014 Oct)
Penglase S,Hamre K,Ellingsen S

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selenow1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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