LAMB2 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	LAMB2
Entrez Gene ID	337767
Full Name	laminin, beta 2 (laminin S)
Synonyms	si:dkey-32e6.2
General protein information	Preferred Names laminin, beta 2 (laminin S) Names laminin subunit beta-2 sof softy
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017353525.1	XP_017209014.1	laminin subunit beta-2 isoform X1
XM_005162046.2	XP_005162103.1	laminin subunit beta-2 isoform X1
NM_001243045.1	NP_001229974.1	laminin subunit beta-2 precursor
XM_017353525.2	XP_017209014.1	laminin subunit beta-2 isoform X1
XM_005162046.3	XP_005162103.1	laminin subunit beta-2 isoform X1
XM_005162045.3	XP_005162102.1	laminin subunit beta-2 isoform X1
XM_005162045.2	XP_005162102.1	laminin subunit beta-2 isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	LAMB2	NP_989497.1
Drosophila melanogaster (fruit fly)	LanB1	NP_723319.1
Danio rerio (zebrafish)	lamb2	NP_001229974.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001381	XP_321757.5
Caenorhabditis elegans (roundworm)	lam-1	NP_500734.3
Mus musculus (house mouse)	Lamb2	NP_032509.2
Homo sapiens (human)	LAMB2	NP_002283.3
Pan troglodytes (chimpanzee)	LAMB2	XP_003309859.1
Bos taurus (cattle)	LAMB2	NP_001039712.2
	LAMB2	XP_001110027.1
Canis lupus familiaris (dog)	LAMB2	XP_005632610.1
Rattus norvegicus (Norway rat)	Lamb2	NP_037106.1
Xenopus tropicalis (tropical clawed frog)	lamb2	XP_002933101.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Canonical Wnt signalling regulates epithelial patterning by modulating levels of laminins in zebrafish appendages.
Nagendran M, Arora P, Gori P, Mulay A, Ray S, Jacob T, Sonawane M
Development (Cambridge, England)142(2)320-30(2015 Jan)

Thrombospondin-4 controls matrix assembly during development and repair of myotendinous junctions.
Subramanian A, Schilling TF
eLife3(2014 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

describe a mutant, softy, which displays severe embryonic muscle degeneration as a result of initial basement membrane failure
Title: The zebrafish dystrophic mutant softy maintains muscle fibre viability despite basement membrane rupture and muscle detachment.

The following LAMB2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LAMB2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa33391	NM_001243045.1 Latest version!	Danio rerio laminin, beta 2 (laminin S) (lamb2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa27565	XM_017353525.2 Latest version!	Danio rerio laminin, beta 2 (laminin S) (lamb2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa27565	XM_005162045.3 Latest version!	Danio rerio laminin, beta 2 (laminin S) (lamb2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa27565	XM_005162046.3 Latest version!	Danio rerio laminin, beta 2 (laminin S) (lamb2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
ODa27565	XM_017353525.1	Danio rerio laminin, beta 2 (laminin S) (lamb2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa27565	XM_005162046.2	Danio rerio laminin, beta 2 (laminin S) (lamb2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa27565	XM_005162045.2	Danio rerio laminin, beta 2 (laminin S) (lamb2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LAMB2 ( NM_001243045.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001229974.1 Danio rerio laminin, beta 2 (laminin S) (lamb2), mRNA.

Price & Availability↑

CloneID	ODa33391
Clone ID Related Accession (Same CDS sequence)	NM_001243045.1
Accession Version	NM_001243045.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5349bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-12
Organism	Danio rerio(zebrafish)
Product	laminin subunit beta-2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL953864.6. On Jul 14, 2011 this sequence version replaced XM_002666455.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GDQH01019217.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCGCGCGC TTCTGTTGCT GTCCATCTGT GTCCTGTCAG CGGTCAGTGC ACAGGAGCCT 
GATGCTGCTC ACGGCTGCAC TCATGGAAGC TGCTATCCTG CAACCGGTGA CCTCTTGGTG 
GGTCGAGAAG CCAACCTGAA AGCATCATCC ACCTGTGGCC TGCGGAGGAG AGAACCCTAC 
TGCATTGTCA GCCATCTGCA GGAGGAGAAG AAGTGTTTCC AGTGTGATTC ACGGCGGCCT 
TATGATCCTC AGTCCAACCC CGTAAGCCAC CGCATCGAGA ACGTCATCAC CACCTTCAAA 
CCACATCGCA AGAAGTCCTG GTGGCAGTCG GAAAACGGGA AGTCTGATGT TTACCTTCAG 
CTGGATCTGG AGGCTGAGTT TCACTTCACT CATCTCATCA TGACTTTTAA GACTTTCCGT 
CCAGCAGCCA TGTTGATTGA GAGATCTGCA GATTTCGGCC GAAGCTGGCA GGTGTATCGC 
TACTTTGCTA ACGACTGCGA GTCCATCTTT CCAGGAATTT CCCAAGATTC TCTTCGAAAG 
GTTGATGATG TCATCTGCGA GTCCAGATAT TCTGATATCG AGCCGTCGAC AGAAGGAGAG 
GTGATTTTTC GTGTTCTTGA CCCTGCTATC AGGATCGAGG ACCCTTACAG CCCAAGAATT 
CAGAACCAGC TGAAGATCAC AAATCTTCGC CTGAATCTTA CCAAGCTGCA CACTCTGGGT 
GACAATCTGC TGGACTCACG GCCTGAGATC AAGGAGAAAT ACTACTATGC CATGTATGAG 
CTGGTCGTCA GGGGCAACTG CTTCTGCTAT GGCCACGCCT CTGAGTGTGC GCCAATTGAG 
GGCATCAGGG ATGACATTGA AGGCATGGTT CACGGGAGGT GTGTGTGCAA ACACAACACT 
AAAGGACTGA ACTGTGAACA GTGTGACGAC TTCTACAATG ATCTGCCCTG GAGACCAGCA 
GAAGGCCGGA ACACTAATGC CTGCAAGAAA TGCAACTGTA ATGGTCACTC CAACCAGTGT 
CATTTTGACA TGGCGGTGTA TTTGGCAACG GGGAACATCA GTGGAGGTGT GTGTGACAAC 
TGCCTTCACA ACACCATGGG CAGCAACTGT GAGAGCTGTA AACCTTTCTA CTACCAGGAT 
CCAACTAGAG ATATTAGAGA CCCTGGAGTC TGTGTAGCTT GCGATTGTGA CCCAGATGGC 
TCTAAGAACG GTGGTGTTTG TGACGGCCAT GATGACCCTT CTCTGGGCAT GATTGCTGGA 
CAGTGCCGCT GTAAAGACAA TGTTGAAGGT TCACGCTGTG ATAAATGCAA GCCTGGTTTC 
TTTGGCCTGA GTGCTAGTGA TCCACGTGGT TGTCAACCAT GTAAATGTGA CCCCAGGGGT 
ACAGTTTCCG GCAGCTCCCA ATGTGACCCT GTGAGCGGAG ACTGCTTCTG TAAACGCCTG 
GTCACTGGAC ACAGCTGCAA CCAATGTCTG CCTGAGCACT GGGCCTTGAG TCATGATACC 
AGTGGCTGCA GAGGGTGTGA CTGTGATGTG GGCGGAGCTA CAGAAAACCA ATGCTCAATG 
GAAACAGGGC AGTGTCAGTG TCGAAGCCAC ATGATTGGTC GCCAGTGCAA CCAGGTGGAG 
TCTGGGTACT TTTTCATGGC ACTTGACCAC TACATCTATG AAGCTGAAAC TGCCAAACTG 
GGTCAAGGCT CTGTTTTTGA GGAGAGAGAG TACCAGCCGG GTCGCCCCAT CTCATGGACA 
GGCACAGGCT TTGCACGAGC TCCTGAGGGC GGGACGCTGG AATTCGCAAT TAACAACATC 
CCATACTCTA TGGAATATGA CGTCCTGATT CGCTATGAGC TTCAGATGCC ACGTGACTGG 
GAAGAGGTGC GTGTTGTAGT GATTAGACCT GGTCCTATAC CTACCAGCAG TCCATGTGGA 
AACACCATCC CTGCAGATGA TCTGCTAACG GTGTCGCTTT CCTCTGGTGC CAGGTTTGTG 
GTTCTGCCTC AGCCAGTGTG TTTGGAGAGT GGTGTGAGTT ACAAACTGCG CGTGGAGCTC 
ATCCGTTACG CTGACAGGAA CAGCATCATT ACCTCCACCA ATGCCTTTGT GCTTGTTGAC 
TCTATCGCAC TTCTCCCCCG TTACTTGTCT CTGGAGATGT TTACTGCAGG AGACCCGGCC 
TCTGTCGCAC GCAAGCAGAG TTTTGAGCGT TACCGCTGCC ACGAAACTGC CAAGAGTGTG 
TCCCGTCCGC AGATGAGTGA AGTGTGTGCC AAACTCATCA CCAGCATGTC TGCCCTCATT 
AATGACGGCG CGCTGGCGTG TGAGTGTGAT CCTCAAGGTT CGCTCAGCTC TGAGTGTGAT 
GTCCGTGGCG GTCAGTGCCG CTGCAGGCCC AACATATCTG GCCGGCGCTG TGAGAAATGT 
GCACCAGGAG CTTACGGCTT TGGCCCATCT GGATGCAAAC TTTGTGAATG CAGTTTAGAG 
GGCTCTCAGA GCCGGTTCTG TGACCTGTAC TCAGGACAGT GTCCATGCCG ACCTGGAGCA 
TATGGTCAGC GCTGTGATGG TTGTCAGGCA GGACACTGGG GTTTCCCAAA CTGCAGGCCA 
TGTCAATGTA ACGGCCATGC TGACGAGTGC CATCAGAGGA CTGGAGCCTG TCTCAACTGC 
AGAAGTAATA CTGCAGGAGA CAAATGTGAA AGGTGTGCTA ATGGCTACTA TGGTAACCCA 
GTCCTTGGTT TAGGGAGTCA GTGTCGGCCC TGCCCCTGTC CTGAGGGCCC CAACAGCGGA 
CGCCACTTTG CTGCCTCTTG TTACCAAGAC AACCGAAGCC AACAGGTGGT CTGCAACTGC 
AACCAGGGTT ACACAGGTCC ACGTTGTGAT GAATGTGCTC CTGGCTATTT TGGTGACCCG 
TCTAAGCCCG GTGGGCAGTG CCAGCCATGT CGTTGCAGTA ATAACATCGA CCTGTCAGAT 
CCTGAGTCTT GTGACAAGCG CACTGGCCAG TGTCTGAAGT GCCTGTACAA CACTGAAGGG 
CCAGACTGTA GTGTGTGCAG GAGCGGATAC TATGGAGACG CTTCTCGACG CAACTGCCGG 
AAGTGCACCT GTAACTTCCT GGGCACAGAG CGCAGTCAGT GTCTGTCTCG AGATGACTGT 
GTGTGTCAGC GTGCCACTGG TCAGTGTCAG TGTCTGCCTA ATGTTATTGG ACAAACCTGT 
GATCACTGCG CTCCAAACTC CTGGAACCTG GCCAGCGGGC AAGGCTGTGA GCCCTGCGCA 
TGTGACCCTA ACAATGCCTA CACATCTGCC TGTAATGAGT TTACAGGCCA GTGCCAGTGC 
CGTGTTGGGT TTGGAGGAAA GACCTGTACA GACTGTCAGG AGAACCACTG GGGTGACCCA 
AGAGTGCTAT GCCGAGCATG TGACTGTGAC CCCCGTGGCA TCGAGTCATC CCAATGCAAC 
CGTATGACGG GTCACTGCGT GTGCCAGCAG GGTGTTTCTG GTGTCCGCTG TGATCAGTGT 
GCCCGTGGCT TCTCCGGGAC CTTCCCTGAC TGTAAGCCTT GCCACCAGTG TTTTGGGGAC 
TGGGACCGTA TCGTTCAGGA TTTGGCCACC AGAACTAAAG CCCTCACAGA TCGAGCCAAG 
GAGCTTCAGA CCACCGGCCT GACTCGAGCA TTCGAGAGAC GCTTTAAGGA GCTGGAAGAC 
ATGCTGGCAC AGGCACGGGA CATTGTTAAT GCCCGTAATG CCACTGCTGA GGCTGTGACC 
ACCCTGATGG GCATGATTGA GGTTCTCAGA GCTCAGATTG GCGAGACCAC AGACACACTG 
AACCAGCAGG AGGGAGACCT GACCGCCGTG CAGGACAGTA ACTATGAAGC CAGTAATGCT 
TTAAGCACAC TGGAGAGAGA AGCAAAAGAG CTCAACCTGA ACGCAGATCA GCTTAACCGT 
CAGCTTGACA TCCTCAAGAA CTCCAACTTC TTGGGTGCAT ATGACAGCAT CCGGGCCTCG 
TATAATAAGT CACGTGACGC AGAGCACCGA TCAAACAGAT CTACCACTGA CACTCCCAGC 
ACAGTCAGCC AATCAGCAGA CACCCGCAAG AAAACAGAGC GGCTGATTGG CCAAAAGAGA 
GATGATTTTA ACCGCAAGAA CGCTGCTAAC AAGCGTACAC TCACTGACTT AAATGCAAAA 
GTTCAGAATC TGGACTTAAA GAAAATCAAC GAAAAGGTGT GTGGAGCTCC AGGTGATGCT 
CAGTGTGTTG ACAGTCCTTG TGGTGGTGCT GGTTGTCGTG ATGATGAAGG CAAGCGTCGA 
TGTGGAGGCC TGAACTGTAA TGGAGCTGTT GCAGTGGCGG ACACTGCTCT GGATAGATCC 
AAACATGCAG AAAAAGAGCT GGATAAAGCA ATGGGGGTAG TGGAGGAGCT TTTTAAGCAG 
GTGGCGGATG CTAAGACCAA GGCACAGGAA GCGAAAGACA GAGCTCAGGC AGCTCTGGAA 
AAAGCTAGCG ATACCAAGAA CAAAGTGGAT CATTCTAACA ATGATCTAAG GGACCTTATC 
AAACAAATAC GCCAATTCCT CATGCAGGAG GGTGCAGATC CGGACAGCAT TGAGGCGGTG 
GCCAATCGTG TGCTGGAGCT CTCAATCCCT GCCTCTCCCA AGCAGATCCG ACACCTAGCA 
GACGAGATTA AAGACCGTGT CAAGAGTCTG TCTAATGTGG ATGCCATTCT GGAGCAAACA 
CAAAATGACG TCCGCAAAGC AGAGCAACTG TTGCTGGATG CCAAGAAAGC TAGGAATAAG 
GCAGAGGGGG TGAAGAACAC AGCTGAGAGT GTGAAAAAGG CCCTGAATGA TGCCAGCAGA 
GCCCAGGCTG CAGCAGAGAA AGCCATCCAG AAAGCTAAGA ATGACATTGG CTTAACTCAG 
AACCAGCTGG CACAGATCCA GTCAGAGACC TCCGCTAGTG AGAGAGATCT TAATGATGCA 
GTGGACAGAC TGGGAGACCT GGAGCGCCAG ATCGAGGCAT TAAAGACCAA ACGTGCCAAC 
AACAGCCTGG ATGCTGCACG GGCAGAGGAG ACGGCCACCA TGGCACGGGA CAAAGCCAAC 
GAAGCCAAAG AGATCCTGGA TGGTGAGCTG TCCGATAAGT ACCGTACTGT TCAGGGTCTG 
ATGGACAATA AGGCGAAAAC CATGCAGGAC GCCAAGCACA AAGCAGAGCA ATTGAGAGAT 
GAAGCCAAGG GGCTGCTGAA AGATGCACAG GATAAACTGC AGAGGCTGGC AGAGCTTGAA 
AAGGACTATG AAGAAAACCA GAAGGTATTG GAAGGCAAAG CCAGGCAGCT GGATGGCCTG 
GAAGATAAAA TGAAGGCGAT CCTGAACGCC ATCAACAGGC AGATTCAGAT CTACAACACC 
TGTCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001229974.1
CDS	117..5465
Translation	MRALLLLSICVLSAVSAQEPDAAHGCTHGSCYPATGDLLVGREANLKASSTCGLRRREPYCIVSHLQEEKKCFQCDSRRPYDPQSNPVSHRIENVITTFKPHRKKSWWQSENGKSDVYLQLDLEAEFHFTHLIMTFKTFRPAAMLIERSADFGRSWQVYRYFANDCESIFPGISQDSLRKVDDVICESRYSDIEPSTEGEVIFRVLDPAIRIEDPYSPRIQNQLKITNLRLNLTKLHTLGDNLLDSRPEIKEKYYYAMYELVVRGNCFCYGHASECAPIEGIRDDIEGMVHGRCVCKHNTKGLNCEQCDDFYNDLPWRPAEGRNTNACKKCNCNGHSNQCHFDMAVYLATGNISGGVCDNCLHNTMGSNCESCKPFYYQDPTRDIRDPGVCVACDCDPDGSKNGGVCDGHDDPSLGMIAGQCRCKDNVEGSRCDKCKPGFFGLSASDPRGCQPCKCDPRGTVSGSSQCDPVSGDCFCKRLVTGHSCNQCLPEHWALSHDTSGCRGCDCDVGGATENQCSMETGQCQCRSHMIGRQCNQVESGYFFMALDHYIYEAETAKLGQGSVFEEREYQPGRPISWTGTGFARAPEGGTLEFAINNIPYSMEYDVLIRYELQMPRDWEEVRVVVIRPGPIPTSSPCGNTIPADDLLTVSLSSGARFVVLPQPVCLESGVSYKLRVELIRYADRNSIITSTNAFVLVDSIALLPRYLSLEMFTAGDPASVARKQSFERYRCHETAKSVSRPQMSEVCAKLITSMSALINDGALACECDPQGSLSSECDVRGGQCRCRPNISGRRCEKCAPGAYGFGPSGCKLCECSLEGSQSRFCDLYSGQCPCRPGAYGQRCDGCQAGHWGFPNCRPCQCNGHADECHQRTGACLNCRSNTAGDKCERCANGYYGNPVLGLGSQCRPCPCPEGPNSGRHFAASCYQDNRSQQVVCNCNQGYTGPRCDECAPGYFGDPSKPGGQCQPCRCSNNIDLSDPESCDKRTGQCLKCLYNTEGPDCSVCRSGYYGDASRRNCRKCTCNFLGTERSQCLSRDDCVCQRATGQCQCLPNVIGQTCDHCAPNSWNLASGQGCEPCACDPNNAYTSACNEFTGQCQCRVGFGGKTCTDCQENHWGDPRVLCRACDCDPRGIESSQCNRMTGHCVCQQGVSGVRCDQCARGFSGTFPDCKPCHQCFGDWDRIVQDLATRTKALTDRAKELQTTGLTRAFERRFKELEDMLAQARDIVNARNATAEAVTTLMGMIEVLRAQIGETTDTLNQQEGDLTAVQDSNYEASNALSTLEREAKELNLNADQLNRQLDILKNSNFLGAYDSIRASYNKSRDAEHRSNRSTTDTPSTVSQSADTRKKTERLIGQKRDDFNRKNAANKRTLTDLNAKVQNLDLKKINEKVCGAPGDAQCVDSPCGGAGCRDDEGKRRCGGLNCNGAVAVADTALDRSKHAEKELDKAMGVVEELFKQVADAKTKAQEAKDRAQAALEKASDTKNKVDHSNNDLRDLIKQIRQFLMQEGADPDSIEAVANRVLELSIPASPKQIRHLADEIKDRVKSLSNVDAILEQTQNDVRKAEQLLLDAKKARNKAEGVKNTAESVKKALNDASRAQAAAEKAIQKAKNDIGLTQNQLAQIQSETSASERDLNDAVDRLGDLERQIEALKTKRANNSLDAARAEETATMARDKANEAKEILDGELSDKYRTVQGLMDNKAKTMQDAKHKAEQLRDEAKGLLKDAQDKLQRLAELEKDYEENQKVLEGKARQLDGLEDKMKAILNAINRQIQIYNTCQ

Target ORF information:

RefSeq Version	NM_001243045.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio laminin, beta 2 (laminin S) (lamb2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001243045.1

ORF Insert Sequence:

ATGCGCGCGC TTCTGTTGCT GTCCATCTGT GTCCTGTCAG CGGTCAGTGC ACAGGAGCCT 
GATGCTGCTC ACGGCTGCAC TCATGGAAGC TGCTATCCTG CAACCGGTGA CCTCTTGGTG 
GGTCGAGAAG CCAACCTGAA AGCATCATCC ACCTGTGGCC TGCGGAGGAG AGAACCCTAC 
TGCATTGTCA GCCATCTGCA GGAGGAGAAG AAGTGTTTCC AGTGTGATTC ACGGCGGCCT 
TATGATCCTC AGTCCAACCC CGTAAGCCAC CGCATCGAGA ACGTCATCAC CACCTTCAAA 
CCACATCGCA AGAAGTCCTG GTGGCAGTCG GAAAACGGGA AGTCTGATGT TTACCTTCAG 
CTGGATCTGG AGGCTGAGTT TCACTTCACT CATCTCATCA TGACTTTTAA GACTTTCCGT 
CCAGCAGCCA TGTTGATTGA GAGATCTGCA GATTTCGGCC GAAGCTGGCA GGTGTATCGC 
TACTTTGCTA ACGACTGCGA GTCCATCTTT CCAGGAATTT CCCAAGATTC TCTTCGAAAG 
GTTGATGATG TCATCTGCGA GTCCAGATAT TCTGATATCG AGCCGTCGAC AGAAGGAGAG 
GTGATTTTTC GTGTTCTTGA CCCTGCTATC AGGATCGAGG ACCCTTACAG CCCAAGAATT 
CAGAACCAGC TGAAGATCAC AAATCTTCGC CTGAATCTTA CCAAGCTGCA CACTCTGGGT 
GACAATCTGC TGGACTCACG GCCTGAGATC AAGGAGAAAT ACTACTATGC CATGTATGAG 
CTGGTCGTCA GGGGCAACTG CTTCTGCTAT GGCCACGCCT CTGAGTGTGC GCCAATTGAG 
GGCATCAGGG ATGACATTGA AGGCATGGTT CACGGGAGGT GTGTGTGCAA ACACAACACT 
AAAGGACTGA ACTGTGAACA GTGTGACGAC TTCTACAATG ATCTGCCCTG GAGACCAGCA 
GAAGGCCGGA ACACTAATGC CTGCAAGAAA TGCAACTGTA ATGGTCACTC CAACCAGTGT 
CATTTTGACA TGGCGGTGTA TTTGGCAACG GGGAACATCA GTGGAGGTGT GTGTGACAAC 
TGCCTTCACA ACACCATGGG CAGCAACTGT GAGAGCTGTA AACCTTTCTA CTACCAGGAT 
CCAACTAGAG ATATTAGAGA CCCTGGAGTC TGTGTAGCTT GCGATTGTGA CCCAGATGGC 
TCTAAGAACG GTGGTGTTTG TGACGGCCAT GATGACCCTT CTCTGGGCAT GATTGCTGGA 
CAGTGCCGCT GTAAAGACAA TGTTGAAGGT TCACGCTGTG ATAAATGCAA GCCTGGTTTC 
TTTGGCCTGA GTGCTAGTGA TCCACGTGGT TGTCAACCAT GTAAATGTGA CCCCAGGGGT 
ACAGTTTCCG GCAGCTCCCA ATGTGACCCT GTGAGCGGAG ACTGCTTCTG TAAACGCCTG 
GTCACTGGAC ACAGCTGCAA CCAATGTCTG CCTGAGCACT GGGCCTTGAG TCATGATACC 
AGTGGCTGCA GAGGGTGTGA CTGTGATGTG GGCGGAGCTA CAGAAAACCA ATGCTCAATG 
GAAACAGGGC AGTGTCAGTG TCGAAGCCAC ATGATTGGTC GCCAGTGCAA CCAGGTGGAG 
TCTGGGTACT TTTTCATGGC ACTTGACCAC TACATCTATG AAGCTGAAAC TGCCAAACTG 
GGTCAAGGCT CTGTTTTTGA GGAGAGAGAG TACCAGCCGG GTCGCCCCAT CTCATGGACA 
GGCACAGGCT TTGCACGAGC TCCTGAGGGC GGGACGCTGG AATTCGCAAT TAACAACATC 
CCATACTCTA TGGAATATGA CGTCCTGATT CGCTATGAGC TTCAGATGCC ACGTGACTGG 
GAAGAGGTGC GTGTTGTAGT GATTAGACCT GGTCCTATAC CTACCAGCAG TCCATGTGGA 
AACACCATCC CTGCAGATGA TCTGCTAACG GTGTCGCTTT CCTCTGGTGC CAGGTTTGTG 
GTTCTGCCTC AGCCAGTGTG TTTGGAGAGT GGTGTGAGTT ACAAACTGCG CGTGGAGCTC 
ATCCGTTACG CTGACAGGAA CAGCATCATT ACCTCCACCA ATGCCTTTGT GCTTGTTGAC 
TCTATCGCAC TTCTCCCCCG TTACTTGTCT CTGGAGATGT TTACTGCAGG AGACCCGGCC 
TCTGTCGCAC GCAAGCAGAG TTTTGAGCGT TACCGCTGCC ACGAAACTGC CAAGAGTGTG 
TCCCGTCCGC AGATGAGTGA AGTGTGTGCC AAACTCATCA CCAGCATGTC TGCCCTCATT 
AATGACGGCG CGCTGGCGTG TGAGTGTGAT CCTCAAGGTT CGCTCAGCTC TGAGTGTGAT 
GTCCGTGGCG GTCAGTGCCG CTGCAGGCCC AACATATCTG GCCGGCGCTG TGAGAAATGT 
GCACCAGGAG CTTACGGCTT TGGCCCATCT GGATGCAAAC TTTGTGAATG CAGTTTAGAG 
GGCTCTCAGA GCCGGTTCTG TGACCTGTAC TCAGGACAGT GTCCATGCCG ACCTGGAGCA 
TATGGTCAGC GCTGTGATGG TTGTCAGGCA GGACACTGGG GTTTCCCAAA CTGCAGGCCA 
TGTCAATGTA ACGGCCATGC TGACGAGTGC CATCAGAGGA CTGGAGCCTG TCTCAACTGC 
AGAAGTAATA CTGCAGGAGA CAAATGTGAA AGGTGTGCTA ATGGCTACTA TGGTAACCCA 
GTCCTTGGTT TAGGGAGTCA GTGTCGGCCC TGCCCCTGTC CTGAGGGCCC CAACAGCGGA 
CGCCACTTTG CTGCCTCTTG TTACCAAGAC AACCGAAGCC AACAGGTGGT CTGCAACTGC 
AACCAGGGTT ACACAGGTCC ACGTTGTGAT GAATGTGCTC CTGGCTATTT TGGTGACCCG 
TCTAAGCCCG GTGGGCAGTG CCAGCCATGT CGTTGCAGTA ATAACATCGA CCTGTCAGAT 
CCTGAGTCTT GTGACAAGCG CACTGGCCAG TGTCTGAAGT GCCTGTACAA CACTGAAGGG 
CCAGACTGTA GTGTGTGCAG GAGCGGATAC TATGGAGACG CTTCTCGACG CAACTGCCGG 
AAGTGCACCT GTAACTTCCT GGGCACAGAG CGCAGTCAGT GTCTGTCTCG AGATGACTGT 
GTGTGTCAGC GTGCCACTGG TCAGTGTCAG TGTCTGCCTA ATGTTATTGG ACAAACCTGT 
GATCACTGCG CTCCAAACTC CTGGAACCTG GCCAGCGGGC AAGGCTGTGA GCCCTGCGCA 
TGTGACCCTA ACAATGCCTA CACATCTGCC TGTAATGAGT TTACAGGCCA GTGCCAGTGC 
CGTGTTGGGT TTGGAGGAAA GACCTGTACA GACTGTCAGG AGAACCACTG GGGTGACCCA 
AGAGTGCTAT GCCGAGCATG TGACTGTGAC CCCCGTGGCA TCGAGTCATC CCAATGCAAC 
CGTATGACGG GTCACTGCGT GTGCCAGCAG GGTGTTTCTG GTGTCCGCTG TGATCAGTGT 
GCCCGTGGCT TCTCCGGGAC CTTCCCTGAC TGTAAGCCTT GCCACCAGTG TTTTGGGGAC 
TGGGACCGTA TCGTTCAGGA TTTGGCCACC AGAACTAAAG CCCTCACAGA TCGAGCCAAG 
GAGCTTCAGA CCACCGGCCT GACTCGAGCA TTCGAGAGAC GCTTTAAGGA GCTGGAAGAC 
ATGCTGGCAC AGGCACGGGA CATTGTTAAT GCCCGTAATG CCACTGCTGA GGCTGTGACC 
ACCCTGATGG GCATGATTGA GGTTCTCAGA GCTCAGATTG GCGAGACCAC AGACACACTG 
AACCAGCAGG AGGGAGACCT GACCGCCGTG CAGGACAGTA ACTATGAAGC CAGTAATGCT 
TTAAGCACAC TGGAGAGAGA AGCAAAAGAG CTCAACCTGA ACGCAGATCA GCTTAACCGT 
CAGCTTGACA TCCTCAAGAA CTCCAACTTC TTGGGTGCAT ATGACAGCAT CCGGGCCTCG 
TATAATAAGT CACGTGACGC AGAGCACCGA TCAAACAGAT CTACCACTGA CACTCCCAGC 
ACAGTCAGCC AATCAGCAGA CACCCGCAAG AAAACAGAGC GGCTGATTGG CCAAAAGAGA 
GATGATTTTA ACCGCAAGAA CGCTGCTAAC AAGCGTACAC TCACTGACTT AAATGCAAAA 
GTTCAGAATC TGGACTTAAA GAAAATCAAC GAAAAGGTGT GTGGAGCTCC AGGTGATGCT 
CAGTGTGTTG ACAGTCCTTG TGGTGGTGCT GGTTGTCGTG ATGATGAAGG CAAGCGTCGA 
TGTGGAGGCC TGAACTGTAA TGGAGCTGTT GCAGTGGCGG ACACTGCTCT GGATAGATCC 
AAACATGCAG AAAAAGAGCT GGATAAAGCA ATGGGGGTAG TGGAGGAGCT TTTTAAGCAG 
GTGGCGGATG CTAAGACCAA GGCACAGGAA GCGAAAGACA GAGCTCAGGC AGCTCTGGAA 
AAAGCTAGCG ATACCAAGAA CAAAGTGGAT CATTCTAACA ATGATCTAAG GGACCTTATC 
AAACAAATAC GCCAATTCCT CATGCAGGAG GGTGCAGATC CGGACAGCAT TGAGGCGGTG 
GCCAATCGTG TGCTGGAGCT CTCAATCCCT GCCTCTCCCA AGCAGATCCG ACACCTAGCA 
GACGAGATTA AAGACCGTGT CAAGAGTCTG TCTAATGTGG ATGCCATTCT GGAGCAAACA 
CAAAATGACG TCCGCAAAGC AGAGCAACTG TTGCTGGATG CCAAGAAAGC TAGGAATAAG 
GCAGAGGGGG TGAAGAACAC AGCTGAGAGT GTGAAAAAGG CCCTGAATGA TGCCAGCAGA 
GCCCAGGCTG CAGCAGAGAA AGCCATCCAG AAAGCTAAGA ATGACATTGG CTTAACTCAG 
AACCAGCTGG CACAGATCCA GTCAGAGACC TCCGCTAGTG AGAGAGATCT TAATGATGCA 
GTGGACAGAC TGGGAGACCT GGAGCGCCAG ATCGAGGCAT TAAAGACCAA ACGTGCCAAC 
AACAGCCTGG ATGCTGCACG GGCAGAGGAG ACGGCCACCA TGGCACGGGA CAAAGCCAAC 
GAAGCCAAAG AGATCCTGGA TGGTGAGCTG TCCGATAAGT ACCGTACTGT TCAGGGTCTG 
ATGGACAATA AGGCGAAAAC CATGCAGGAC GCCAAGCACA AAGCAGAGCA ATTGAGAGAT 
GAAGCCAAGG GGCTGCTGAA AGATGCACAG GATAAACTGC AGAGGCTGGC AGAGCTTGAA 
AAGGACTATG AAGAAAACCA GAAGGTATTG GAAGGCAAAG CCAGGCAGCT GGATGGCCTG 
GAAGATAAAA TGAAGGCGAT CCTGAACGCC ATCAACAGGC AGATTCAGAT CTACAACACC 
TGTCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LAMB2 ( XM_017353525.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017209014.1 Danio rerio laminin, beta 2 (laminin S) (lamb2), transcript variant X2, mRNA.

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CloneID	ODa27565
Clone ID Related Accession (Same CDS sequence)	XM_017353525.1 , XM_005162046.2 , XM_017353525.2 , XM_005162046.3 , XM_005162045.3 , XM_005162045.2
Accession Version	XM_017353525.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	laminin subunit beta-2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGCGC TTCTGTTGCT GTCCATCTGT GTCCTGTCAG CGGTCAGTGC ACAGGAGCCT 
GATGCTGCTC ACGGCTGCAC TCATGGAAGC TGCTATCCTG CAACCGGTGA CCTCTTGGTG 
GGTCGAGAAG CCAACCTGAA AGCATCATCC ACCTGTGGCC TGCGGAGGAG AGAACCCTAC 
TGCATTGTCA GCCATCTGCA GGAGGAGAAG AAGTGTTTCC AGTGTGATTC ACGGCGGCCT 
TATGATCCTC AGTCCAACCC CGTAAGCCAC CGCATCGAGA ACGTCATCAC CACCTTCAAA 
CCACATCGCA AGAAGTCCTG GTGGCAGTCG GAAAACGGGA AGTCTGATGT TTACCTTCAG 
CTGGATCTGG AGGCTGAGTT TCACTTCACT CATCTCATCA TGACTTTTAA GACTTTCCGT 
CCAGCAGCCA TGTTGATTGA GAGATCTGCA GATTTCGGCC GAAGCTGGCA GGTGTATCGC 
TACTTTGCTA ACGACTGCGA GTCCATCTTT CCAGGAATTT CCCAAGATTC TCTTCGAAAG 
GTTGATGATG TCATCTGCGA GTCCAGATAT TCTGATATCG AGCCGTCGAC AGAAGGAGAG 
GTGATTTTTC GTGTTCTTGA CCCTGCTATC AGGATCGAGG ACCCTTACAG CCCAAGAATT 
CAGAACCAGC TGAAGATCAC AAATCTTCGC CTGAATCTTA CCAAGCTGCA CACTCTGGGT 
GACAATCTGC TGGACTCACG GCCTGAGATC AAGGAGAAAT ACTACTATGC CATGTATGAG 
CTGGTCGTCA GGGGCAACTG CTTCTGCTAT GGCCACGCCT CTGAGTGTGC GCCAATTGAG 
GGCATCAGGG ATGACATTGA AGGCATGGTT CACGGGAGGT GTGTGTGCAA ACACAACACT 
AAAGGACTGA ACTGTGAACA GTGTGACGAC TTCTACAATG ATCTGCCCTG GAGACCAGCA 
GAAGGCCGGA ACACTAATGC CTGCAAGAAA TGCAACTGTA ATGGTCACTC CAACCAGTGT 
CATTTTGACA TGGCGGTGTA TTTGGCAACG GGGAACATCA GTGGAGGTGT GTGTGACAAC 
TGCCTTCACA ACACCATGGG CAGCAACTGT GAGAGCTGTA AACCTTTCTA CTACCAGGAT 
CCAACTAGAG ATATTAGAGA CCCTGGAGTC TGTGTAGCTT GCGATTGTGA CCCAGATGGC 
TCTAAGAACG GTGGTGTTTG TGACGGCCAT GATGACCCTT CTCTGGGCAT GATTGCTGGA 
CAGTGCCGCT GTAAAGACAA TGTTGAAGGT TCACGCTGTG ATAAATGCAA GCCTGGTTTC 
TTTGGCCTGA GTGCTAGTGA TCCACGTGGT TGTCAACCAT GTAAATGTGA CCCCAGGGGT 
ACAGTTTCCG GCAGCTCCCA ATGTGACCCT GTGAGCGGAG ACTGCTTCTG TAAACGCCTG 
GTCACTGGAC ACAGCTGCAA CCAATGTCTG CCTGAGCACT GGGCCTTGAG TCATGATACC 
AGTGGCTGCA GAGGGTGTGA CTGTGATGTG GGCGGAGCTA CAGAAAACCA ATGCTCAATG 
GAAACAGGGC AGTGTCAGTG TCGAAGCCAC ATGATTGGTC GCCAGTGCAA CCAGGTGGAG 
TCTGGGTACT TTTTCATGGC ACTTGACCAC TACATCTATG AAGCTGAAAC TGCCAAACTG 
GGTCAAGGCT CTGTTTTTGA GGAGAGAGAG TACCAGCCGG GTCGCCCCAT CTCATGGACA 
GGCACAGGCT TTGCACGAGC TCCTGAGGGC GGGACGCTGG AATTCGCAAT TAACAACATC 
CCATACTCTA TGGAATATGA CGTCCTGATT CGCTATGAGC TTCAGATGCC ACGTGACTGG 
GAAGAGGTGC GTGTTGTAGT GATTAGACCT GGTCCTATAC CTACCAGCAG TCCATGTGGA 
AACACCATCC CTGCAGATGA TCTGCTAACG GTGTCGCTTT CCTCTGGTGC CAGGTTTGTG 
GTTCTGCCTC AGCCAGTGTG TTTGGAGAGT GGTGTGAGTT ACAAACTGCG CGTGGAGCTC 
ATCCGTTACG CTGACAGGAA CAGCATCATT ACCTCCACCA ATGCCTTTGT GCTTGTTGAC 
TCTATCGCAC TTCTCCCCCG TTACTTGTCT CTGGAGATGT TTACTGCAGG AGACCCGGCC 
TCTGTCGCAC GCAAGCAGAG TTTTGAGCGT TACCGCTGCC ACGAAACTGC CAAGAGTGTG 
TCCCGTCCGC AGATGAGTGA AGTGTGTGCC AAACTCATCA CCAGCATGTC TGCCCTCATT 
AATGACGGCG CGCTGGCGTG TGAGTGTGAT CCTCAAGGTT CGCTCAGCTC TGAGTGTGAT 
GTCCGTGGCG GTCAGTGCCG CTGCAGGCCC AACATATCTG GCCGGCGCTG TGAGAAATGT 
GCACCAGGAG CTTACGGCTT TGGCCCATCT GGATGCAAAC TTTGTGAATG CAGTTTAGAG 
GGCTCTCAGA GCCGGTTCTG TGACCTGTAC TCAGGACAGT GTCCATGCCG ACCTGGAGCA 
TATGGTCAGC GCTGTGATGG TTGTCAGGCA GGACACTGGG GTTTCCCAAA CTGCAGGCCA 
TGTCAATGTA ACGGCCATGC TGACGAGTGC CATCAGAGGA CTGGAGCCTG TCTCAACTGC 
AGAAGTAATA CTGCAGGAGA CAAATGTGAA AGGTGTGCTA ATGGCTACTA TGGTAACCCA 
GTCCTTGGTT TAGGGAGTCA GTGTCGGCCC TGCCCCTGTC CTGAGGGCCC CAACAGCGGA 
CGCCACTTTG CTGCCTCTTG TTACCAAGAC AACCGAAGCC AACAGGTGGT CTGCAACTGC 
AACCAGGGTT ACACAGGGAC TGTAAAGCGC TCTGCAGGGG CAGTCCTCAA TCGTCCACGT 
TGTGATGAAT GTGCTCCTGG CTATTTTGGT GACCCGTCTA AGCCCGGTGG GCAGTGCCAG 
CCATGTCGTT GCAGTAATAA CATCGACCTG TCAGATCCTG AGTCTTGTGA CAAGCGCACT 
GGCCAGTGTC TGAAGTGCCT GTACAACACT GAAGGGCCAG ACTGTAGTGT GTGCAGGAGC 
GGATACTATG GAGACGCTTC TCGACGCAAC TGCCGGAAGT GCACCTGTAA CTTCCTGGGC 
ACAGAGCGCA GTCAGTGTCT GTCTCGAGAT GACTGTGTGT GTCAGCGTGC CACTGGTCAG 
TGTCAGTGTC TGCCTAATGT TATTGGACAA ACCTGTGATC ACTGCGCTCC AAACTCCTGG 
AACCTGGCCA GCGGGCAAGG CTGTGAGCCC TGCGCATGTG ACCCTAACAA TGCCTACACA 
TCTGCCTGTA ATGAGTTTAC AGGCCAGTGC CAGTGCCGTG TTGGGTTTGG AGGAAAGACC 
TGTACAGACT GTCAGGAGAA CCACTGGGGT GACCCAAGAG TGCTATGCCG AGCATGTGAC 
TGTGACCCCC GTGGCATCGA GTCATCCCAA TGCAACCGTA TGACGGGTCA CTGCGTGTGC 
CAGCAGGGTG TTTCTGGTGT CCGCTGTGAT CAGTGTGCCC GTGGCTTCTC CGGGACCTTC 
CCTGACTGTA AGCCTTGCCA CCAGTGTTTT GGGGACTGGG ACCGTATCGT TCAGGATTTG 
GCCACCAGAA CTAAAGCCCT CACAGATCGA GCCAAGGAGC TTCAGACCAC CGGCCTGACT 
CGAGCATTCG AGAGACGCTT TAAGGAGCTG GAAGACATGC TGGCACAGGC ACGGGACATT 
GTTAATGCCC GTAATGCCAC TGCTGAGGCT GTGACCACCC TGATGGGCAT GATTGAGGTT 
CTCAGAGCTC AGATTGGCGA GACCACAGAC ACACTGAACC AGCAGGAGGG AGACCTGACC 
GCCGTGCAGG ACAGTAACTA TGAAGCCAGT AATGCTTTAA GCACACTGGA GAGAGAAGCA 
AAAGAGCTCA ACCTGAACGC AGATCAGCTT AACCGTCAGC TTGACATCCT CAAGAACTCC 
AACTTCTTGG GTGCATATGA CAGCATCCGG GCCTCGTATA ATAAGTCACG TGACGCAGAG 
CACCGATCAA ACAGATCTAC CACTGACACT CCCAGCACAG TCAGCCAATC AGCAGACACC 
CGCAAGAAAA CAGAGCGGCT GATTGGCCAA AAGAGAGATG ATTTTAACCG CAAGAACGCT 
GCTAACAAGC GTACACTCAC TGACTTAAAT GCAAAAGTTC AGAATCTGGA CTTAAAGAAA 
ATCAACGAAA AGGTGTGTGG AGCTCCAGGT GATGCTCAGT GTGTTGACAG TCCTTGTGGT 
GGTGCTGGTT GTCGTGATGA TGAAGGCAAG CGTCGATGTG GAGGCCTGAA CTGTAATGGA 
GCTGTTGCAG TGGCGGACAC TGCTCTGGAT AGATCCAAAC ATGCAGAAAA AGAGCTGGAT 
AAAGCAATGG GGGTAGTGGA GGAGCTTTTT AAGCAGGTGG CGGATGCTAA GACCAAGGCA 
CAGGAAGCGA AAGACAGAGC TCAGGCAGCT CTGGAAAAAG CTAGCGATAC CAAGAACAAA 
GTGGATCATT CTAACAATGA TCTAAGGGAC CTTATCAAAC AAATACGCCA ATTCCTCATG 
CAGGAGGGTG CAGATCCGGA CAGCATTGAG GCGGTGGCCA ATCGTGTGCT GGAGCTCTCA 
ATCCCTGCCT CTCCCAAGCA GATCCGACAC CTAGCAGACG AGATTAAAGA CCGTGTCAAG 
AGTCTGTCTA ATGTGGATGC CATTCTGGAG CAAACACAAA ATGACGTCCG CAAAGCAGAG 
CAACTGTTGC TGGATGCCAA GAAAGCTAGG AATAAGGCAG AGGGGGTGAA GAACACAGCT 
GAGAGTGTGA AAAAGGCCCT GAATGATGCC AGCAGAGCCC AGGCTGCAGC AGAGAAAGCC 
ATCCAGAAAG CTAAGAATGA CATTGGCTTA ACTCAGAACC AGCTGGCACA GATCCAGTCA 
GAGACCTCCG CTAGTGAGAG AGATCTTAAT GATGCAGTGG ACAGACTGGG AGACCTGGAG 
CGCCAGATCG AGGCATTAAA GACCAAACGT GCCAACAACA GCCTGGATGC TGCACGGGCA 
GAGGAGACGG CCACCATGGC ACGGGACAAA GCCAACGAAG CCAAAGAGAT CCTGGATGGT 
GAGCTGTCCG ATAAGTACCG TACTGTTCAG GGTCTGATGG ACAATAAGGC GAAAACCATG 
CAGGACGCCA AGCACAAAGC AGAGCAATTG AGAGATGAAG CCAAGGGGCT GCTGAAAGAT 
GCACAGGATA AACTGCAGAG GCTGGCAGAG CTTGAAAAGG ACTATGAAGA AAACCAGAAG 
GTATTGGAAG GCAAAGCCAG GCAGCTGGAT GGCCTGGAAG ATAAAATGAA GGCGATCCTG 
AACGCCATCA ACAGGCAGAT TCAGATCTAC AACACCTGTC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209014.1
CDS	1317..6701
Translation	MRALLLLSICVLSAVSAQEPDAAHGCTHGSCYPATGDLLVGREANLKASSTCGLRRREPYCIVSHLQEEKKCFQCDSRRPYDPQSNPVSHRIENVITTFKPHRKKSWWQSENGKSDVYLQLDLEAEFHFTHLIMTFKTFRPAAMLIERSADFGRSWQVYRYFANDCESIFPGISQDSLRKVDDVICESRYSDIEPSTEGEVIFRVLDPAIRIEDPYSPRIQNQLKITNLRLNLTKLHTLGDNLLDSRPEIKEKYYYAMYELVVRGNCFCYGHASECAPIEGIRDDIEGMVHGRCVCKHNTKGLNCEQCDDFYNDLPWRPAEGRNTNACKKCNCNGHSNQCHFDMAVYLATGNISGGVCDNCLHNTMGSNCESCKPFYYQDPTRDIRDPGVCVACDCDPDGSKNGGVCDGHDDPSLGMIAGQCRCKDNVEGSRCDKCKPGFFGLSASDPRGCQPCKCDPRGTVSGSSQCDPVSGDCFCKRLVTGHSCNQCLPEHWALSHDTSGCRGCDCDVGGATENQCSMETGQCQCRSHMIGRQCNQVESGYFFMALDHYIYEAETAKLGQGSVFEEREYQPGRPISWTGTGFARAPEGGTLEFAINNIPYSMEYDVLIRYELQMPRDWEEVRVVVIRPGPIPTSSPCGNTIPADDLLTVSLSSGARFVVLPQPVCLESGVSYKLRVELIRYADRNSIITSTNAFVLVDSIALLPRYLSLEMFTAGDPASVARKQSFERYRCHETAKSVSRPQMSEVCAKLITSMSALINDGALACECDPQGSLSSECDVRGGQCRCRPNISGRRCEKCAPGAYGFGPSGCKLCECSLEGSQSRFCDLYSGQCPCRPGAYGQRCDGCQAGHWGFPNCRPCQCNGHADECHQRTGACLNCRSNTAGDKCERCANGYYGNPVLGLGSQCRPCPCPEGPNSGRHFAASCYQDNRSQQVVCNCNQGYTGTVKRSAGAVLNRPRCDECAPGYFGDPSKPGGQCQPCRCSNNIDLSDPESCDKRTGQCLKCLYNTEGPDCSVCRSGYYGDASRRNCRKCTCNFLGTERSQCLSRDDCVCQRATGQCQCLPNVIGQTCDHCAPNSWNLASGQGCEPCACDPNNAYTSACNEFTGQCQCRVGFGGKTCTDCQENHWGDPRVLCRACDCDPRGIESSQCNRMTGHCVCQQGVSGVRCDQCARGFSGTFPDCKPCHQCFGDWDRIVQDLATRTKALTDRAKELQTTGLTRAFERRFKELEDMLAQARDIVNARNATAEAVTTLMGMIEVLRAQIGETTDTLNQQEGDLTAVQDSNYEASNALSTLEREAKELNLNADQLNRQLDILKNSNFLGAYDSIRASYNKSRDAEHRSNRSTTDTPSTVSQSADTRKKTERLIGQKRDDFNRKNAANKRTLTDLNAKVQNLDLKKINEKVCGAPGDAQCVDSPCGGAGCRDDEGKRRCGGLNCNGAVAVADTALDRSKHAEKELDKAMGVVEELFKQVADAKTKAQEAKDRAQAALEKASDTKNKVDHSNNDLRDLIKQIRQFLMQEGADPDSIEAVANRVLELSIPASPKQIRHLADEIKDRVKSLSNVDAILEQTQNDVRKAEQLLLDAKKARNKAEGVKNTAESVKKALNDASRAQAAAEKAIQKAKNDIGLTQNQLAQIQSETSASERDLNDAVDRLGDLERQIEALKTKRANNSLDAARAEETATMARDKANEAKEILDGELSDKYRTVQGLMDNKAKTMQDAKHKAEQLRDEAKGLLKDAQDKLQRLAELEKDYEENQKVLEGKARQLDGLEDKMKAILNAINRQIQIYNTCQ

Target ORF information:

RefSeq Version	XM_017353525.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio laminin, beta 2 (laminin S) (lamb2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353525.1

ORF Insert Sequence:

ATGCGCGCGC TTCTGTTGCT GTCCATCTGT GTCCTGTCAG CGGTCAGTGC ACAGGAGCCT 
GATGCTGCTC ACGGCTGCAC TCATGGAAGC TGCTATCCTG CAACCGGTGA CCTCTTGGTG 
GGTCGAGAAG CCAACCTGAA AGCATCATCC ACCTGTGGCC TGCGGAGGAG AGAACCCTAC 
TGCATTGTCA GCCATCTGCA GGAGGAGAAG AAGTGTTTCC AGTGTGATTC ACGGCGGCCT 
TATGATCCTC AGTCCAACCC CGTAAGCCAC CGCATCGAGA ACGTCATCAC CACCTTCAAA 
CCACATCGCA AGAAGTCCTG GTGGCAGTCG GAAAACGGGA AGTCTGATGT TTACCTTCAG 
CTGGATCTGG AGGCTGAGTT TCACTTCACT CATCTCATCA TGACTTTTAA GACTTTCCGT 
CCAGCAGCCA TGTTGATTGA GAGATCTGCA GATTTCGGCC GAAGCTGGCA GGTGTATCGC 
TACTTTGCTA ACGACTGCGA GTCCATCTTT CCAGGAATTT CCCAAGATTC TCTTCGAAAG 
GTTGATGATG TCATCTGCGA GTCCAGATAT TCTGATATCG AGCCGTCGAC AGAAGGAGAG 
GTGATTTTTC GTGTTCTTGA CCCTGCTATC AGGATCGAGG ACCCTTACAG CCCAAGAATT 
CAGAACCAGC TGAAGATCAC AAATCTTCGC CTGAATCTTA CCAAGCTGCA CACTCTGGGT 
GACAATCTGC TGGACTCACG GCCTGAGATC AAGGAGAAAT ACTACTATGC CATGTATGAG 
CTGGTCGTCA GGGGCAACTG CTTCTGCTAT GGCCACGCCT CTGAGTGTGC GCCAATTGAG 
GGCATCAGGG ATGACATTGA AGGCATGGTT CACGGGAGGT GTGTGTGCAA ACACAACACT 
AAAGGACTGA ACTGTGAACA GTGTGACGAC TTCTACAATG ATCTGCCCTG GAGACCAGCA 
GAAGGCCGGA ACACTAATGC CTGCAAGAAA TGCAACTGTA ATGGTCACTC CAACCAGTGT 
CATTTTGACA TGGCGGTGTA TTTGGCAACG GGGAACATCA GTGGAGGTGT GTGTGACAAC 
TGCCTTCACA ACACCATGGG CAGCAACTGT GAGAGCTGTA AACCTTTCTA CTACCAGGAT 
CCAACTAGAG ATATTAGAGA CCCTGGAGTC TGTGTAGCTT GCGATTGTGA CCCAGATGGC 
TCTAAGAACG GTGGTGTTTG TGACGGCCAT GATGACCCTT CTCTGGGCAT GATTGCTGGA 
CAGTGCCGCT GTAAAGACAA TGTTGAAGGT TCACGCTGTG ATAAATGCAA GCCTGGTTTC 
TTTGGCCTGA GTGCTAGTGA TCCACGTGGT TGTCAACCAT GTAAATGTGA CCCCAGGGGT 
ACAGTTTCCG GCAGCTCCCA ATGTGACCCT GTGAGCGGAG ACTGCTTCTG TAAACGCCTG 
GTCACTGGAC ACAGCTGCAA CCAATGTCTG CCTGAGCACT GGGCCTTGAG TCATGATACC 
AGTGGCTGCA GAGGGTGTGA CTGTGATGTG GGCGGAGCTA CAGAAAACCA ATGCTCAATG 
GAAACAGGGC AGTGTCAGTG TCGAAGCCAC ATGATTGGTC GCCAGTGCAA CCAGGTGGAG 
TCTGGGTACT TTTTCATGGC ACTTGACCAC TACATCTATG AAGCTGAAAC TGCCAAACTG 
GGTCAAGGCT CTGTTTTTGA GGAGAGAGAG TACCAGCCGG GTCGCCCCAT CTCATGGACA 
GGCACAGGCT TTGCACGAGC TCCTGAGGGC GGGACGCTGG AATTCGCAAT TAACAACATC 
CCATACTCTA TGGAATATGA CGTCCTGATT CGCTATGAGC TTCAGATGCC ACGTGACTGG 
GAAGAGGTGC GTGTTGTAGT GATTAGACCT GGTCCTATAC CTACCAGCAG TCCATGTGGA 
AACACCATCC CTGCAGATGA TCTGCTAACG GTGTCGCTTT CCTCTGGTGC CAGGTTTGTG 
GTTCTGCCTC AGCCAGTGTG TTTGGAGAGT GGTGTGAGTT ACAAACTGCG CGTGGAGCTC 
ATCCGTTACG CTGACAGGAA CAGCATCATT ACCTCCACCA ATGCCTTTGT GCTTGTTGAC 
TCTATCGCAC TTCTCCCCCG TTACTTGTCT CTGGAGATGT TTACTGCAGG AGACCCGGCC 
TCTGTCGCAC GCAAGCAGAG TTTTGAGCGT TACCGCTGCC ACGAAACTGC CAAGAGTGTG 
TCCCGTCCGC AGATGAGTGA AGTGTGTGCC AAACTCATCA CCAGCATGTC TGCCCTCATT 
AATGACGGCG CGCTGGCGTG TGAGTGTGAT CCTCAAGGTT CGCTCAGCTC TGAGTGTGAT 
GTCCGTGGCG GTCAGTGCCG CTGCAGGCCC AACATATCTG GCCGGCGCTG TGAGAAATGT 
GCACCAGGAG CTTACGGCTT TGGCCCATCT GGATGCAAAC TTTGTGAATG CAGTTTAGAG 
GGCTCTCAGA GCCGGTTCTG TGACCTGTAC TCAGGACAGT GTCCATGCCG ACCTGGAGCA 
TATGGTCAGC GCTGTGATGG TTGTCAGGCA GGACACTGGG GTTTCCCAAA CTGCAGGCCA 
TGTCAATGTA ACGGCCATGC TGACGAGTGC CATCAGAGGA CTGGAGCCTG TCTCAACTGC 
AGAAGTAATA CTGCAGGAGA CAAATGTGAA AGGTGTGCTA ATGGCTACTA TGGTAACCCA 
GTCCTTGGTT TAGGGAGTCA GTGTCGGCCC TGCCCCTGTC CTGAGGGCCC CAACAGCGGA 
CGCCACTTTG CTGCCTCTTG TTACCAAGAC AACCGAAGCC AACAGGTGGT CTGCAACTGC 
AACCAGGGTT ACACAGGGAC TGTAAAGCGC TCTGCAGGGG CAGTCCTCAA TCGTCCACGT 
TGTGATGAAT GTGCTCCTGG CTATTTTGGT GACCCGTCTA AGCCCGGTGG GCAGTGCCAG 
CCATGTCGTT GCAGTAATAA CATCGACCTG TCAGATCCTG AGTCTTGTGA CAAGCGCACT 
GGCCAGTGTC TGAAGTGCCT GTACAACACT GAAGGGCCAG ACTGTAGTGT GTGCAGGAGC 
GGATACTATG GAGACGCTTC TCGACGCAAC TGCCGGAAGT GCACCTGTAA CTTCCTGGGC 
ACAGAGCGCA GTCAGTGTCT GTCTCGAGAT GACTGTGTGT GTCAGCGTGC CACTGGTCAG 
TGTCAGTGTC TGCCTAATGT TATTGGACAA ACCTGTGATC ACTGCGCTCC AAACTCCTGG 
AACCTGGCCA GCGGGCAAGG CTGTGAGCCC TGCGCATGTG ACCCTAACAA TGCCTACACA 
TCTGCCTGTA ATGAGTTTAC AGGCCAGTGC CAGTGCCGTG TTGGGTTTGG AGGAAAGACC 
TGTACAGACT GTCAGGAGAA CCACTGGGGT GACCCAAGAG TGCTATGCCG AGCATGTGAC 
TGTGACCCCC GTGGCATCGA GTCATCCCAA TGCAACCGTA TGACGGGTCA CTGCGTGTGC 
CAGCAGGGTG TTTCTGGTGT CCGCTGTGAT CAGTGTGCCC GTGGCTTCTC CGGGACCTTC 
CCTGACTGTA AGCCTTGCCA CCAGTGTTTT GGGGACTGGG ACCGTATCGT TCAGGATTTG 
GCCACCAGAA CTAAAGCCCT CACAGATCGA GCCAAGGAGC TTCAGACCAC CGGCCTGACT 
CGAGCATTCG AGAGACGCTT TAAGGAGCTG GAAGACATGC TGGCACAGGC ACGGGACATT 
GTTAATGCCC GTAATGCCAC TGCTGAGGCT GTGACCACCC TGATGGGCAT GATTGAGGTT 
CTCAGAGCTC AGATTGGCGA GACCACAGAC ACACTGAACC AGCAGGAGGG AGACCTGACC 
GCCGTGCAGG ACAGTAACTA TGAAGCCAGT AATGCTTTAA GCACACTGGA GAGAGAAGCA 
AAAGAGCTCA ACCTGAACGC AGATCAGCTT AACCGTCAGC TTGACATCCT CAAGAACTCC 
AACTTCTTGG GTGCATATGA CAGCATCCGG GCCTCGTATA ATAAGTCACG TGACGCAGAG 
CACCGATCAA ACAGATCTAC CACTGACACT CCCAGCACAG TCAGCCAATC AGCAGACACC 
CGCAAGAAAA CAGAGCGGCT GATTGGCCAA AAGAGAGATG ATTTTAACCG CAAGAACGCT 
GCTAACAAGC GTACACTCAC TGACTTAAAT GCAAAAGTTC AGAATCTGGA CTTAAAGAAA 
ATCAACGAAA AGGTGTGTGG AGCTCCAGGT GATGCTCAGT GTGTTGACAG TCCTTGTGGT 
GGTGCTGGTT GTCGTGATGA TGAAGGCAAG CGTCGATGTG GAGGCCTGAA CTGTAATGGA 
GCTGTTGCAG TGGCGGACAC TGCTCTGGAT AGATCCAAAC ATGCAGAAAA AGAGCTGGAT 
AAAGCAATGG GGGTAGTGGA GGAGCTTTTT AAGCAGGTGG CGGATGCTAA GACCAAGGCA 
CAGGAAGCGA AAGACAGAGC TCAGGCAGCT CTGGAAAAAG CTAGCGATAC CAAGAACAAA 
GTGGATCATT CTAACAATGA TCTAAGGGAC CTTATCAAAC AAATACGCCA ATTCCTCATG 
CAGGAGGGTG CAGATCCGGA CAGCATTGAG GCGGTGGCCA ATCGTGTGCT GGAGCTCTCA 
ATCCCTGCCT CTCCCAAGCA GATCCGACAC CTAGCAGACG AGATTAAAGA CCGTGTCAAG 
AGTCTGTCTA ATGTGGATGC CATTCTGGAG CAAACACAAA ATGACGTCCG CAAAGCAGAG 
CAACTGTTGC TGGATGCCAA GAAAGCTAGG AATAAGGCAG AGGGGGTGAA GAACACAGCT 
GAGAGTGTGA AAAAGGCCCT GAATGATGCC AGCAGAGCCC AGGCTGCAGC AGAGAAAGCC 
ATCCAGAAAG CTAAGAATGA CATTGGCTTA ACTCAGAACC AGCTGGCACA GATCCAGTCA 
GAGACCTCCG CTAGTGAGAG AGATCTTAAT GATGCAGTGG ACAGACTGGG AGACCTGGAG 
CGCCAGATCG AGGCATTAAA GACCAAACGT GCCAACAACA GCCTGGATGC TGCACGGGCA 
GAGGAGACGG CCACCATGGC ACGGGACAAA GCCAACGAAG CCAAAGAGAT CCTGGATGGT 
GAGCTGTCCG ATAAGTACCG TACTGTTCAG GGTCTGATGG ACAATAAGGC GAAAACCATG 
CAGGACGCCA AGCACAAAGC AGAGCAATTG AGAGATGAAG CCAAGGGGCT GCTGAAAGAT 
GCACAGGATA AACTGCAGAG GCTGGCAGAG CTTGAAAAGG ACTATGAAGA AAACCAGAAG 
GTATTGGAAG GCAAAGCCAG GCAGCTGGAT GGCCTGGAAG ATAAAATGAA GGCGATCCTG 
AACGCCATCA ACAGGCAGAT TCAGATCTAC AACACCTGTC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LAMB2 ( XM_005162046.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005162103.1 Danio rerio laminin, beta 2 (laminin S) (lamb2), transcript variant X3, mRNA.

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CloneID	ODa27565
Clone ID Related Accession (Same CDS sequence)	XM_017353525.1 , XM_005162046.2 , XM_017353525.2 , XM_005162046.3 , XM_005162045.3 , XM_005162045.2
Accession Version	XM_005162046.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	laminin subunit beta-2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2014 this sequence version replaced XM_005162046.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGCGC TTCTGTTGCT GTCCATCTGT GTCCTGTCAG CGGTCAGTGC ACAGGAGCCT 
GATGCTGCTC ACGGCTGCAC TCATGGAAGC TGCTATCCTG CAACCGGTGA CCTCTTGGTG 
GGTCGAGAAG CCAACCTGAA AGCATCATCC ACCTGTGGCC TGCGGAGGAG AGAACCCTAC 
TGCATTGTCA GCCATCTGCA GGAGGAGAAG AAGTGTTTCC AGTGTGATTC ACGGCGGCCT 
TATGATCCTC AGTCCAACCC CGTAAGCCAC CGCATCGAGA ACGTCATCAC CACCTTCAAA 
CCACATCGCA AGAAGTCCTG GTGGCAGTCG GAAAACGGGA AGTCTGATGT TTACCTTCAG 
CTGGATCTGG AGGCTGAGTT TCACTTCACT CATCTCATCA TGACTTTTAA GACTTTCCGT 
CCAGCAGCCA TGTTGATTGA GAGATCTGCA GATTTCGGCC GAAGCTGGCA GGTGTATCGC 
TACTTTGCTA ACGACTGCGA GTCCATCTTT CCAGGAATTT CCCAAGATTC TCTTCGAAAG 
GTTGATGATG TCATCTGCGA GTCCAGATAT TCTGATATCG AGCCGTCGAC AGAAGGAGAG 
GTGATTTTTC GTGTTCTTGA CCCTGCTATC AGGATCGAGG ACCCTTACAG CCCAAGAATT 
CAGAACCAGC TGAAGATCAC AAATCTTCGC CTGAATCTTA CCAAGCTGCA CACTCTGGGT 
GACAATCTGC TGGACTCACG GCCTGAGATC AAGGAGAAAT ACTACTATGC CATGTATGAG 
CTGGTCGTCA GGGGCAACTG CTTCTGCTAT GGCCACGCCT CTGAGTGTGC GCCAATTGAG 
GGCATCAGGG ATGACATTGA AGGCATGGTT CACGGGAGGT GTGTGTGCAA ACACAACACT 
AAAGGACTGA ACTGTGAACA GTGTGACGAC TTCTACAATG ATCTGCCCTG GAGACCAGCA 
GAAGGCCGGA ACACTAATGC CTGCAAGAAA TGCAACTGTA ATGGTCACTC CAACCAGTGT 
CATTTTGACA TGGCGGTGTA TTTGGCAACG GGGAACATCA GTGGAGGTGT GTGTGACAAC 
TGCCTTCACA ACACCATGGG CAGCAACTGT GAGAGCTGTA AACCTTTCTA CTACCAGGAT 
CCAACTAGAG ATATTAGAGA CCCTGGAGTC TGTGTAGCTT GCGATTGTGA CCCAGATGGC 
TCTAAGAACG GTGGTGTTTG TGACGGCCAT GATGACCCTT CTCTGGGCAT GATTGCTGGA 
CAGTGCCGCT GTAAAGACAA TGTTGAAGGT TCACGCTGTG ATAAATGCAA GCCTGGTTTC 
TTTGGCCTGA GTGCTAGTGA TCCACGTGGT TGTCAACCAT GTAAATGTGA CCCCAGGGGT 
ACAGTTTCCG GCAGCTCCCA ATGTGACCCT GTGAGCGGAG ACTGCTTCTG TAAACGCCTG 
GTCACTGGAC ACAGCTGCAA CCAATGTCTG CCTGAGCACT GGGCCTTGAG TCATGATACC 
AGTGGCTGCA GAGGGTGTGA CTGTGATGTG GGCGGAGCTA CAGAAAACCA ATGCTCAATG 
GAAACAGGGC AGTGTCAGTG TCGAAGCCAC ATGATTGGTC GCCAGTGCAA CCAGGTGGAG 
TCTGGGTACT TTTTCATGGC ACTTGACCAC TACATCTATG AAGCTGAAAC TGCCAAACTG 
GGTCAAGGCT CTGTTTTTGA GGAGAGAGAG TACCAGCCGG GTCGCCCCAT CTCATGGACA 
GGCACAGGCT TTGCACGAGC TCCTGAGGGC GGGACGCTGG AATTCGCAAT TAACAACATC 
CCATACTCTA TGGAATATGA CGTCCTGATT CGCTATGAGC TTCAGATGCC ACGTGACTGG 
GAAGAGGTGC GTGTTGTAGT GATTAGACCT GGTCCTATAC CTACCAGCAG TCCATGTGGA 
AACACCATCC CTGCAGATGA TCTGCTAACG GTGTCGCTTT CCTCTGGTGC CAGGTTTGTG 
GTTCTGCCTC AGCCAGTGTG TTTGGAGAGT GGTGTGAGTT ACAAACTGCG CGTGGAGCTC 
ATCCGTTACG CTGACAGGAA CAGCATCATT ACCTCCACCA ATGCCTTTGT GCTTGTTGAC 
TCTATCGCAC TTCTCCCCCG TTACTTGTCT CTGGAGATGT TTACTGCAGG AGACCCGGCC 
TCTGTCGCAC GCAAGCAGAG TTTTGAGCGT TACCGCTGCC ACGAAACTGC CAAGAGTGTG 
TCCCGTCCGC AGATGAGTGA AGTGTGTGCC AAACTCATCA CCAGCATGTC TGCCCTCATT 
AATGACGGCG CGCTGGCGTG TGAGTGTGAT CCTCAAGGTT CGCTCAGCTC TGAGTGTGAT 
GTCCGTGGCG GTCAGTGCCG CTGCAGGCCC AACATATCTG GCCGGCGCTG TGAGAAATGT 
GCACCAGGAG CTTACGGCTT TGGCCCATCT GGATGCAAAC TTTGTGAATG CAGTTTAGAG 
GGCTCTCAGA GCCGGTTCTG TGACCTGTAC TCAGGACAGT GTCCATGCCG ACCTGGAGCA 
TATGGTCAGC GCTGTGATGG TTGTCAGGCA GGACACTGGG GTTTCCCAAA CTGCAGGCCA 
TGTCAATGTA ACGGCCATGC TGACGAGTGC CATCAGAGGA CTGGAGCCTG TCTCAACTGC 
AGAAGTAATA CTGCAGGAGA CAAATGTGAA AGGTGTGCTA ATGGCTACTA TGGTAACCCA 
GTCCTTGGTT TAGGGAGTCA GTGTCGGCCC TGCCCCTGTC CTGAGGGCCC CAACAGCGGA 
CGCCACTTTG CTGCCTCTTG TTACCAAGAC AACCGAAGCC AACAGGTGGT CTGCAACTGC 
AACCAGGGTT ACACAGGGAC TGTAAAGCGC TCTGCAGGGG CAGTCCTCAA TCGTCCACGT 
TGTGATGAAT GTGCTCCTGG CTATTTTGGT GACCCGTCTA AGCCCGGTGG GCAGTGCCAG 
CCATGTCGTT GCAGTAATAA CATCGACCTG TCAGATCCTG AGTCTTGTGA CAAGCGCACT 
GGCCAGTGTC TGAAGTGCCT GTACAACACT GAAGGGCCAG ACTGTAGTGT GTGCAGGAGC 
GGATACTATG GAGACGCTTC TCGACGCAAC TGCCGGAAGT GCACCTGTAA CTTCCTGGGC 
ACAGAGCGCA GTCAGTGTCT GTCTCGAGAT GACTGTGTGT GTCAGCGTGC CACTGGTCAG 
TGTCAGTGTC TGCCTAATGT TATTGGACAA ACCTGTGATC ACTGCGCTCC AAACTCCTGG 
AACCTGGCCA GCGGGCAAGG CTGTGAGCCC TGCGCATGTG ACCCTAACAA TGCCTACACA 
TCTGCCTGTA ATGAGTTTAC AGGCCAGTGC CAGTGCCGTG TTGGGTTTGG AGGAAAGACC 
TGTACAGACT GTCAGGAGAA CCACTGGGGT GACCCAAGAG TGCTATGCCG AGCATGTGAC 
TGTGACCCCC GTGGCATCGA GTCATCCCAA TGCAACCGTA TGACGGGTCA CTGCGTGTGC 
CAGCAGGGTG TTTCTGGTGT CCGCTGTGAT CAGTGTGCCC GTGGCTTCTC CGGGACCTTC 
CCTGACTGTA AGCCTTGCCA CCAGTGTTTT GGGGACTGGG ACCGTATCGT TCAGGATTTG 
GCCACCAGAA CTAAAGCCCT CACAGATCGA GCCAAGGAGC TTCAGACCAC CGGCCTGACT 
CGAGCATTCG AGAGACGCTT TAAGGAGCTG GAAGACATGC TGGCACAGGC ACGGGACATT 
GTTAATGCCC GTAATGCCAC TGCTGAGGCT GTGACCACCC TGATGGGCAT GATTGAGGTT 
CTCAGAGCTC AGATTGGCGA GACCACAGAC ACACTGAACC AGCAGGAGGG AGACCTGACC 
GCCGTGCAGG ACAGTAACTA TGAAGCCAGT AATGCTTTAA GCACACTGGA GAGAGAAGCA 
AAAGAGCTCA ACCTGAACGC AGATCAGCTT AACCGTCAGC TTGACATCCT CAAGAACTCC 
AACTTCTTGG GTGCATATGA CAGCATCCGG GCCTCGTATA ATAAGTCACG TGACGCAGAG 
CACCGATCAA ACAGATCTAC CACTGACACT CCCAGCACAG TCAGCCAATC AGCAGACACC 
CGCAAGAAAA CAGAGCGGCT GATTGGCCAA AAGAGAGATG ATTTTAACCG CAAGAACGCT 
GCTAACAAGC GTACACTCAC TGACTTAAAT GCAAAAGTTC AGAATCTGGA CTTAAAGAAA 
ATCAACGAAA AGGTGTGTGG AGCTCCAGGT GATGCTCAGT GTGTTGACAG TCCTTGTGGT 
GGTGCTGGTT GTCGTGATGA TGAAGGCAAG CGTCGATGTG GAGGCCTGAA CTGTAATGGA 
GCTGTTGCAG TGGCGGACAC TGCTCTGGAT AGATCCAAAC ATGCAGAAAA AGAGCTGGAT 
AAAGCAATGG GGGTAGTGGA GGAGCTTTTT AAGCAGGTGG CGGATGCTAA GACCAAGGCA 
CAGGAAGCGA AAGACAGAGC TCAGGCAGCT CTGGAAAAAG CTAGCGATAC CAAGAACAAA 
GTGGATCATT CTAACAATGA TCTAAGGGAC CTTATCAAAC AAATACGCCA ATTCCTCATG 
CAGGAGGGTG CAGATCCGGA CAGCATTGAG GCGGTGGCCA ATCGTGTGCT GGAGCTCTCA 
ATCCCTGCCT CTCCCAAGCA GATCCGACAC CTAGCAGACG AGATTAAAGA CCGTGTCAAG 
AGTCTGTCTA ATGTGGATGC CATTCTGGAG CAAACACAAA ATGACGTCCG CAAAGCAGAG 
CAACTGTTGC TGGATGCCAA GAAAGCTAGG AATAAGGCAG AGGGGGTGAA GAACACAGCT 
GAGAGTGTGA AAAAGGCCCT GAATGATGCC AGCAGAGCCC AGGCTGCAGC AGAGAAAGCC 
ATCCAGAAAG CTAAGAATGA CATTGGCTTA ACTCAGAACC AGCTGGCACA GATCCAGTCA 
GAGACCTCCG CTAGTGAGAG AGATCTTAAT GATGCAGTGG ACAGACTGGG AGACCTGGAG 
CGCCAGATCG AGGCATTAAA GACCAAACGT GCCAACAACA GCCTGGATGC TGCACGGGCA 
GAGGAGACGG CCACCATGGC ACGGGACAAA GCCAACGAAG CCAAAGAGAT CCTGGATGGT 
GAGCTGTCCG ATAAGTACCG TACTGTTCAG GGTCTGATGG ACAATAAGGC GAAAACCATG 
CAGGACGCCA AGCACAAAGC AGAGCAATTG AGAGATGAAG CCAAGGGGCT GCTGAAAGAT 
GCACAGGATA AACTGCAGAG GCTGGCAGAG CTTGAAAAGG ACTATGAAGA AAACCAGAAG 
GTATTGGAAG GCAAAGCCAG GCAGCTGGAT GGCCTGGAAG ATAAAATGAA GGCGATCCTG 
AACGCCATCA ACAGGCAGAT TCAGATCTAC AACACCTGTC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162103.1
CDS	1199..6583
Translation	MRALLLLSICVLSAVSAQEPDAAHGCTHGSCYPATGDLLVGREANLKASSTCGLRRREPYCIVSHLQEEKKCFQCDSRRPYDPQSNPVSHRIENVITTFKPHRKKSWWQSENGKSDVYLQLDLEAEFHFTHLIMTFKTFRPAAMLIERSADFGRSWQVYRYFANDCESIFPGISQDSLRKVDDVICESRYSDIEPSTEGEVIFRVLDPAIRIEDPYSPRIQNQLKITNLRLNLTKLHTLGDNLLDSRPEIKEKYYYAMYELVVRGNCFCYGHASECAPIEGIRDDIEGMVHGRCVCKHNTKGLNCEQCDDFYNDLPWRPAEGRNTNACKKCNCNGHSNQCHFDMAVYLATGNISGGVCDNCLHNTMGSNCESCKPFYYQDPTRDIRDPGVCVACDCDPDGSKNGGVCDGHDDPSLGMIAGQCRCKDNVEGSRCDKCKPGFFGLSASDPRGCQPCKCDPRGTVSGSSQCDPVSGDCFCKRLVTGHSCNQCLPEHWALSHDTSGCRGCDCDVGGATENQCSMETGQCQCRSHMIGRQCNQVESGYFFMALDHYIYEAETAKLGQGSVFEEREYQPGRPISWTGTGFARAPEGGTLEFAINNIPYSMEYDVLIRYELQMPRDWEEVRVVVIRPGPIPTSSPCGNTIPADDLLTVSLSSGARFVVLPQPVCLESGVSYKLRVELIRYADRNSIITSTNAFVLVDSIALLPRYLSLEMFTAGDPASVARKQSFERYRCHETAKSVSRPQMSEVCAKLITSMSALINDGALACECDPQGSLSSECDVRGGQCRCRPNISGRRCEKCAPGAYGFGPSGCKLCECSLEGSQSRFCDLYSGQCPCRPGAYGQRCDGCQAGHWGFPNCRPCQCNGHADECHQRTGACLNCRSNTAGDKCERCANGYYGNPVLGLGSQCRPCPCPEGPNSGRHFAASCYQDNRSQQVVCNCNQGYTGTVKRSAGAVLNRPRCDECAPGYFGDPSKPGGQCQPCRCSNNIDLSDPESCDKRTGQCLKCLYNTEGPDCSVCRSGYYGDASRRNCRKCTCNFLGTERSQCLSRDDCVCQRATGQCQCLPNVIGQTCDHCAPNSWNLASGQGCEPCACDPNNAYTSACNEFTGQCQCRVGFGGKTCTDCQENHWGDPRVLCRACDCDPRGIESSQCNRMTGHCVCQQGVSGVRCDQCARGFSGTFPDCKPCHQCFGDWDRIVQDLATRTKALTDRAKELQTTGLTRAFERRFKELEDMLAQARDIVNARNATAEAVTTLMGMIEVLRAQIGETTDTLNQQEGDLTAVQDSNYEASNALSTLEREAKELNLNADQLNRQLDILKNSNFLGAYDSIRASYNKSRDAEHRSNRSTTDTPSTVSQSADTRKKTERLIGQKRDDFNRKNAANKRTLTDLNAKVQNLDLKKINEKVCGAPGDAQCVDSPCGGAGCRDDEGKRRCGGLNCNGAVAVADTALDRSKHAEKELDKAMGVVEELFKQVADAKTKAQEAKDRAQAALEKASDTKNKVDHSNNDLRDLIKQIRQFLMQEGADPDSIEAVANRVLELSIPASPKQIRHLADEIKDRVKSLSNVDAILEQTQNDVRKAEQLLLDAKKARNKAEGVKNTAESVKKALNDASRAQAAAEKAIQKAKNDIGLTQNQLAQIQSETSASERDLNDAVDRLGDLERQIEALKTKRANNSLDAARAEETATMARDKANEAKEILDGELSDKYRTVQGLMDNKAKTMQDAKHKAEQLRDEAKGLLKDAQDKLQRLAELEKDYEENQKVLEGKARQLDGLEDKMKAILNAINRQIQIYNTCQ

Target ORF information:

RefSeq Version	XM_005162046.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio laminin, beta 2 (laminin S) (lamb2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162046.2

ORF Insert Sequence:

ATGCGCGCGC TTCTGTTGCT GTCCATCTGT GTCCTGTCAG CGGTCAGTGC ACAGGAGCCT 
GATGCTGCTC ACGGCTGCAC TCATGGAAGC TGCTATCCTG CAACCGGTGA CCTCTTGGTG 
GGTCGAGAAG CCAACCTGAA AGCATCATCC ACCTGTGGCC TGCGGAGGAG AGAACCCTAC 
TGCATTGTCA GCCATCTGCA GGAGGAGAAG AAGTGTTTCC AGTGTGATTC ACGGCGGCCT 
TATGATCCTC AGTCCAACCC CGTAAGCCAC CGCATCGAGA ACGTCATCAC CACCTTCAAA 
CCACATCGCA AGAAGTCCTG GTGGCAGTCG GAAAACGGGA AGTCTGATGT TTACCTTCAG 
CTGGATCTGG AGGCTGAGTT TCACTTCACT CATCTCATCA TGACTTTTAA GACTTTCCGT 
CCAGCAGCCA TGTTGATTGA GAGATCTGCA GATTTCGGCC GAAGCTGGCA GGTGTATCGC 
TACTTTGCTA ACGACTGCGA GTCCATCTTT CCAGGAATTT CCCAAGATTC TCTTCGAAAG 
GTTGATGATG TCATCTGCGA GTCCAGATAT TCTGATATCG AGCCGTCGAC AGAAGGAGAG 
GTGATTTTTC GTGTTCTTGA CCCTGCTATC AGGATCGAGG ACCCTTACAG CCCAAGAATT 
CAGAACCAGC TGAAGATCAC AAATCTTCGC CTGAATCTTA CCAAGCTGCA CACTCTGGGT 
GACAATCTGC TGGACTCACG GCCTGAGATC AAGGAGAAAT ACTACTATGC CATGTATGAG 
CTGGTCGTCA GGGGCAACTG CTTCTGCTAT GGCCACGCCT CTGAGTGTGC GCCAATTGAG 
GGCATCAGGG ATGACATTGA AGGCATGGTT CACGGGAGGT GTGTGTGCAA ACACAACACT 
AAAGGACTGA ACTGTGAACA GTGTGACGAC TTCTACAATG ATCTGCCCTG GAGACCAGCA 
GAAGGCCGGA ACACTAATGC CTGCAAGAAA TGCAACTGTA ATGGTCACTC CAACCAGTGT 
CATTTTGACA TGGCGGTGTA TTTGGCAACG GGGAACATCA GTGGAGGTGT GTGTGACAAC 
TGCCTTCACA ACACCATGGG CAGCAACTGT GAGAGCTGTA AACCTTTCTA CTACCAGGAT 
CCAACTAGAG ATATTAGAGA CCCTGGAGTC TGTGTAGCTT GCGATTGTGA CCCAGATGGC 
TCTAAGAACG GTGGTGTTTG TGACGGCCAT GATGACCCTT CTCTGGGCAT GATTGCTGGA 
CAGTGCCGCT GTAAAGACAA TGTTGAAGGT TCACGCTGTG ATAAATGCAA GCCTGGTTTC 
TTTGGCCTGA GTGCTAGTGA TCCACGTGGT TGTCAACCAT GTAAATGTGA CCCCAGGGGT 
ACAGTTTCCG GCAGCTCCCA ATGTGACCCT GTGAGCGGAG ACTGCTTCTG TAAACGCCTG 
GTCACTGGAC ACAGCTGCAA CCAATGTCTG CCTGAGCACT GGGCCTTGAG TCATGATACC 
AGTGGCTGCA GAGGGTGTGA CTGTGATGTG GGCGGAGCTA CAGAAAACCA ATGCTCAATG 
GAAACAGGGC AGTGTCAGTG TCGAAGCCAC ATGATTGGTC GCCAGTGCAA CCAGGTGGAG 
TCTGGGTACT TTTTCATGGC ACTTGACCAC TACATCTATG AAGCTGAAAC TGCCAAACTG 
GGTCAAGGCT CTGTTTTTGA GGAGAGAGAG TACCAGCCGG GTCGCCCCAT CTCATGGACA 
GGCACAGGCT TTGCACGAGC TCCTGAGGGC GGGACGCTGG AATTCGCAAT TAACAACATC 
CCATACTCTA TGGAATATGA CGTCCTGATT CGCTATGAGC TTCAGATGCC ACGTGACTGG 
GAAGAGGTGC GTGTTGTAGT GATTAGACCT GGTCCTATAC CTACCAGCAG TCCATGTGGA 
AACACCATCC CTGCAGATGA TCTGCTAACG GTGTCGCTTT CCTCTGGTGC CAGGTTTGTG 
GTTCTGCCTC AGCCAGTGTG TTTGGAGAGT GGTGTGAGTT ACAAACTGCG CGTGGAGCTC 
ATCCGTTACG CTGACAGGAA CAGCATCATT ACCTCCACCA ATGCCTTTGT GCTTGTTGAC 
TCTATCGCAC TTCTCCCCCG TTACTTGTCT CTGGAGATGT TTACTGCAGG AGACCCGGCC 
TCTGTCGCAC GCAAGCAGAG TTTTGAGCGT TACCGCTGCC ACGAAACTGC CAAGAGTGTG 
TCCCGTCCGC AGATGAGTGA AGTGTGTGCC AAACTCATCA CCAGCATGTC TGCCCTCATT 
AATGACGGCG CGCTGGCGTG TGAGTGTGAT CCTCAAGGTT CGCTCAGCTC TGAGTGTGAT 
GTCCGTGGCG GTCAGTGCCG CTGCAGGCCC AACATATCTG GCCGGCGCTG TGAGAAATGT 
GCACCAGGAG CTTACGGCTT TGGCCCATCT GGATGCAAAC TTTGTGAATG CAGTTTAGAG 
GGCTCTCAGA GCCGGTTCTG TGACCTGTAC TCAGGACAGT GTCCATGCCG ACCTGGAGCA 
TATGGTCAGC GCTGTGATGG TTGTCAGGCA GGACACTGGG GTTTCCCAAA CTGCAGGCCA 
TGTCAATGTA ACGGCCATGC TGACGAGTGC CATCAGAGGA CTGGAGCCTG TCTCAACTGC 
AGAAGTAATA CTGCAGGAGA CAAATGTGAA AGGTGTGCTA ATGGCTACTA TGGTAACCCA 
GTCCTTGGTT TAGGGAGTCA GTGTCGGCCC TGCCCCTGTC CTGAGGGCCC CAACAGCGGA 
CGCCACTTTG CTGCCTCTTG TTACCAAGAC AACCGAAGCC AACAGGTGGT CTGCAACTGC 
AACCAGGGTT ACACAGGGAC TGTAAAGCGC TCTGCAGGGG CAGTCCTCAA TCGTCCACGT 
TGTGATGAAT GTGCTCCTGG CTATTTTGGT GACCCGTCTA AGCCCGGTGG GCAGTGCCAG 
CCATGTCGTT GCAGTAATAA CATCGACCTG TCAGATCCTG AGTCTTGTGA CAAGCGCACT 
GGCCAGTGTC TGAAGTGCCT GTACAACACT GAAGGGCCAG ACTGTAGTGT GTGCAGGAGC 
GGATACTATG GAGACGCTTC TCGACGCAAC TGCCGGAAGT GCACCTGTAA CTTCCTGGGC 
ACAGAGCGCA GTCAGTGTCT GTCTCGAGAT GACTGTGTGT GTCAGCGTGC CACTGGTCAG 
TGTCAGTGTC TGCCTAATGT TATTGGACAA ACCTGTGATC ACTGCGCTCC AAACTCCTGG 
AACCTGGCCA GCGGGCAAGG CTGTGAGCCC TGCGCATGTG ACCCTAACAA TGCCTACACA 
TCTGCCTGTA ATGAGTTTAC AGGCCAGTGC CAGTGCCGTG TTGGGTTTGG AGGAAAGACC 
TGTACAGACT GTCAGGAGAA CCACTGGGGT GACCCAAGAG TGCTATGCCG AGCATGTGAC 
TGTGACCCCC GTGGCATCGA GTCATCCCAA TGCAACCGTA TGACGGGTCA CTGCGTGTGC 
CAGCAGGGTG TTTCTGGTGT CCGCTGTGAT CAGTGTGCCC GTGGCTTCTC CGGGACCTTC 
CCTGACTGTA AGCCTTGCCA CCAGTGTTTT GGGGACTGGG ACCGTATCGT TCAGGATTTG 
GCCACCAGAA CTAAAGCCCT CACAGATCGA GCCAAGGAGC TTCAGACCAC CGGCCTGACT 
CGAGCATTCG AGAGACGCTT TAAGGAGCTG GAAGACATGC TGGCACAGGC ACGGGACATT 
GTTAATGCCC GTAATGCCAC TGCTGAGGCT GTGACCACCC TGATGGGCAT GATTGAGGTT 
CTCAGAGCTC AGATTGGCGA GACCACAGAC ACACTGAACC AGCAGGAGGG AGACCTGACC 
GCCGTGCAGG ACAGTAACTA TGAAGCCAGT AATGCTTTAA GCACACTGGA GAGAGAAGCA 
AAAGAGCTCA ACCTGAACGC AGATCAGCTT AACCGTCAGC TTGACATCCT CAAGAACTCC 
AACTTCTTGG GTGCATATGA CAGCATCCGG GCCTCGTATA ATAAGTCACG TGACGCAGAG 
CACCGATCAA ACAGATCTAC CACTGACACT CCCAGCACAG TCAGCCAATC AGCAGACACC 
CGCAAGAAAA CAGAGCGGCT GATTGGCCAA AAGAGAGATG ATTTTAACCG CAAGAACGCT 
GCTAACAAGC GTACACTCAC TGACTTAAAT GCAAAAGTTC AGAATCTGGA CTTAAAGAAA 
ATCAACGAAA AGGTGTGTGG AGCTCCAGGT GATGCTCAGT GTGTTGACAG TCCTTGTGGT 
GGTGCTGGTT GTCGTGATGA TGAAGGCAAG CGTCGATGTG GAGGCCTGAA CTGTAATGGA 
GCTGTTGCAG TGGCGGACAC TGCTCTGGAT AGATCCAAAC ATGCAGAAAA AGAGCTGGAT 
AAAGCAATGG GGGTAGTGGA GGAGCTTTTT AAGCAGGTGG CGGATGCTAA GACCAAGGCA 
CAGGAAGCGA AAGACAGAGC TCAGGCAGCT CTGGAAAAAG CTAGCGATAC CAAGAACAAA 
GTGGATCATT CTAACAATGA TCTAAGGGAC CTTATCAAAC AAATACGCCA ATTCCTCATG 
CAGGAGGGTG CAGATCCGGA CAGCATTGAG GCGGTGGCCA ATCGTGTGCT GGAGCTCTCA 
ATCCCTGCCT CTCCCAAGCA GATCCGACAC CTAGCAGACG AGATTAAAGA CCGTGTCAAG 
AGTCTGTCTA ATGTGGATGC CATTCTGGAG CAAACACAAA ATGACGTCCG CAAAGCAGAG 
CAACTGTTGC TGGATGCCAA GAAAGCTAGG AATAAGGCAG AGGGGGTGAA GAACACAGCT 
GAGAGTGTGA AAAAGGCCCT GAATGATGCC AGCAGAGCCC AGGCTGCAGC AGAGAAAGCC 
ATCCAGAAAG CTAAGAATGA CATTGGCTTA ACTCAGAACC AGCTGGCACA GATCCAGTCA 
GAGACCTCCG CTAGTGAGAG AGATCTTAAT GATGCAGTGG ACAGACTGGG AGACCTGGAG 
CGCCAGATCG AGGCATTAAA GACCAAACGT GCCAACAACA GCCTGGATGC TGCACGGGCA 
GAGGAGACGG CCACCATGGC ACGGGACAAA GCCAACGAAG CCAAAGAGAT CCTGGATGGT 
GAGCTGTCCG ATAAGTACCG TACTGTTCAG GGTCTGATGG ACAATAAGGC GAAAACCATG 
CAGGACGCCA AGCACAAAGC AGAGCAATTG AGAGATGAAG CCAAGGGGCT GCTGAAAGAT 
GCACAGGATA AACTGCAGAG GCTGGCAGAG CTTGAAAAGG ACTATGAAGA AAACCAGAAG 
GTATTGGAAG GCAAAGCCAG GCAGCTGGAT GGCCTGGAAG ATAAAATGAA GGCGATCCTG 
AACGCCATCA ACAGGCAGAT TCAGATCTAC AACACCTGTC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LAMB2 ( XM_017353525.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017209014.1 Danio rerio laminin, beta 2 (laminin S) (lamb2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa27565
Clone ID Related Accession (Same CDS sequence)	XM_017353525.1 , XM_005162046.2 , XM_017353525.2 , XM_005162046.3 , XM_005162045.3 , XM_005162045.2
Accession Version	XM_017353525.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	laminin subunit beta-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017353525.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGCGC TTCTGTTGCT GTCCATCTGT GTCCTGTCAG CGGTCAGTGC ACAGGAGCCT 
GATGCTGCTC ACGGCTGCAC TCATGGAAGC TGCTATCCTG CAACCGGTGA CCTCTTGGTG 
GGTCGAGAAG CCAACCTGAA AGCATCATCC ACCTGTGGCC TGCGGAGGAG AGAACCCTAC 
TGCATTGTCA GCCATCTGCA GGAGGAGAAG AAGTGTTTCC AGTGTGATTC ACGGCGGCCT 
TATGATCCTC AGTCCAACCC CGTAAGCCAC CGCATCGAGA ACGTCATCAC CACCTTCAAA 
CCACATCGCA AGAAGTCCTG GTGGCAGTCG GAAAACGGGA AGTCTGATGT TTACCTTCAG 
CTGGATCTGG AGGCTGAGTT TCACTTCACT CATCTCATCA TGACTTTTAA GACTTTCCGT 
CCAGCAGCCA TGTTGATTGA GAGATCTGCA GATTTCGGCC GAAGCTGGCA GGTGTATCGC 
TACTTTGCTA ACGACTGCGA GTCCATCTTT CCAGGAATTT CCCAAGATTC TCTTCGAAAG 
GTTGATGATG TCATCTGCGA GTCCAGATAT TCTGATATCG AGCCGTCGAC AGAAGGAGAG 
GTGATTTTTC GTGTTCTTGA CCCTGCTATC AGGATCGAGG ACCCTTACAG CCCAAGAATT 
CAGAACCAGC TGAAGATCAC AAATCTTCGC CTGAATCTTA CCAAGCTGCA CACTCTGGGT 
GACAATCTGC TGGACTCACG GCCTGAGATC AAGGAGAAAT ACTACTATGC CATGTATGAG 
CTGGTCGTCA GGGGCAACTG CTTCTGCTAT GGCCACGCCT CTGAGTGTGC GCCAATTGAG 
GGCATCAGGG ATGACATTGA AGGCATGGTT CACGGGAGGT GTGTGTGCAA ACACAACACT 
AAAGGACTGA ACTGTGAACA GTGTGACGAC TTCTACAATG ATCTGCCCTG GAGACCAGCA 
GAAGGCCGGA ACACTAATGC CTGCAAGAAA TGCAACTGTA ATGGTCACTC CAACCAGTGT 
CATTTTGACA TGGCGGTGTA TTTGGCAACG GGGAACATCA GTGGAGGTGT GTGTGACAAC 
TGCCTTCACA ACACCATGGG CAGCAACTGT GAGAGCTGTA AACCTTTCTA CTACCAGGAT 
CCAACTAGAG ATATTAGAGA CCCTGGAGTC TGTGTAGCTT GCGATTGTGA CCCAGATGGC 
TCTAAGAACG GTGGTGTTTG TGACGGCCAT GATGACCCTT CTCTGGGCAT GATTGCTGGA 
CAGTGCCGCT GTAAAGACAA TGTTGAAGGT TCACGCTGTG ATAAATGCAA GCCTGGTTTC 
TTTGGCCTGA GTGCTAGTGA TCCACGTGGT TGTCAACCAT GTAAATGTGA CCCCAGGGGT 
ACAGTTTCCG GCAGCTCCCA ATGTGACCCT GTGAGCGGAG ACTGCTTCTG TAAACGCCTG 
GTCACTGGAC ACAGCTGCAA CCAATGTCTG CCTGAGCACT GGGCCTTGAG TCATGATACC 
AGTGGCTGCA GAGGGTGTGA CTGTGATGTG GGCGGAGCTA CAGAAAACCA ATGCTCAATG 
GAAACAGGGC AGTGTCAGTG TCGAAGCCAC ATGATTGGTC GCCAGTGCAA CCAGGTGGAG 
TCTGGGTACT TTTTCATGGC ACTTGACCAC TACATCTATG AAGCTGAAAC TGCCAAACTG 
GGTCAAGGCT CTGTTTTTGA GGAGAGAGAG TACCAGCCGG GTCGCCCCAT CTCATGGACA 
GGCACAGGCT TTGCACGAGC TCCTGAGGGC GGGACGCTGG AATTCGCAAT TAACAACATC 
CCATACTCTA TGGAATATGA CGTCCTGATT CGCTATGAGC TTCAGATGCC ACGTGACTGG 
GAAGAGGTGC GTGTTGTAGT GATTAGACCT GGTCCTATAC CTACCAGCAG TCCATGTGGA 
AACACCATCC CTGCAGATGA TCTGCTAACG GTGTCGCTTT CCTCTGGTGC CAGGTTTGTG 
GTTCTGCCTC AGCCAGTGTG TTTGGAGAGT GGTGTGAGTT ACAAACTGCG CGTGGAGCTC 
ATCCGTTACG CTGACAGGAA CAGCATCATT ACCTCCACCA ATGCCTTTGT GCTTGTTGAC 
TCTATCGCAC TTCTCCCCCG TTACTTGTCT CTGGAGATGT TTACTGCAGG AGACCCGGCC 
TCTGTCGCAC GCAAGCAGAG TTTTGAGCGT TACCGCTGCC ACGAAACTGC CAAGAGTGTG 
TCCCGTCCGC AGATGAGTGA AGTGTGTGCC AAACTCATCA CCAGCATGTC TGCCCTCATT 
AATGACGGCG CGCTGGCGTG TGAGTGTGAT CCTCAAGGTT CGCTCAGCTC TGAGTGTGAT 
GTCCGTGGCG GTCAGTGCCG CTGCAGGCCC AACATATCTG GCCGGCGCTG TGAGAAATGT 
GCACCAGGAG CTTACGGCTT TGGCCCATCT GGATGCAAAC TTTGTGAATG CAGTTTAGAG 
GGCTCTCAGA GCCGGTTCTG TGACCTGTAC TCAGGACAGT GTCCATGCCG ACCTGGAGCA 
TATGGTCAGC GCTGTGATGG TTGTCAGGCA GGACACTGGG GTTTCCCAAA CTGCAGGCCA 
TGTCAATGTA ACGGCCATGC TGACGAGTGC CATCAGAGGA CTGGAGCCTG TCTCAACTGC 
AGAAGTAATA CTGCAGGAGA CAAATGTGAA AGGTGTGCTA ATGGCTACTA TGGTAACCCA 
GTCCTTGGTT TAGGGAGTCA GTGTCGGCCC TGCCCCTGTC CTGAGGGCCC CAACAGCGGA 
CGCCACTTTG CTGCCTCTTG TTACCAAGAC AACCGAAGCC AACAGGTGGT CTGCAACTGC 
AACCAGGGTT ACACAGGGAC TGTAAAGCGC TCTGCAGGGG CAGTCCTCAA TCGTCCACGT 
TGTGATGAAT GTGCTCCTGG CTATTTTGGT GACCCGTCTA AGCCCGGTGG GCAGTGCCAG 
CCATGTCGTT GCAGTAATAA CATCGACCTG TCAGATCCTG AGTCTTGTGA CAAGCGCACT 
GGCCAGTGTC TGAAGTGCCT GTACAACACT GAAGGGCCAG ACTGTAGTGT GTGCAGGAGC 
GGATACTATG GAGACGCTTC TCGACGCAAC TGCCGGAAGT GCACCTGTAA CTTCCTGGGC 
ACAGAGCGCA GTCAGTGTCT GTCTCGAGAT GACTGTGTGT GTCAGCGTGC CACTGGTCAG 
TGTCAGTGTC TGCCTAATGT TATTGGACAA ACCTGTGATC ACTGCGCTCC AAACTCCTGG 
AACCTGGCCA GCGGGCAAGG CTGTGAGCCC TGCGCATGTG ACCCTAACAA TGCCTACACA 
TCTGCCTGTA ATGAGTTTAC AGGCCAGTGC CAGTGCCGTG TTGGGTTTGG AGGAAAGACC 
TGTACAGACT GTCAGGAGAA CCACTGGGGT GACCCAAGAG TGCTATGCCG AGCATGTGAC 
TGTGACCCCC GTGGCATCGA GTCATCCCAA TGCAACCGTA TGACGGGTCA CTGCGTGTGC 
CAGCAGGGTG TTTCTGGTGT CCGCTGTGAT CAGTGTGCCC GTGGCTTCTC CGGGACCTTC 
CCTGACTGTA AGCCTTGCCA CCAGTGTTTT GGGGACTGGG ACCGTATCGT TCAGGATTTG 
GCCACCAGAA CTAAAGCCCT CACAGATCGA GCCAAGGAGC TTCAGACCAC CGGCCTGACT 
CGAGCATTCG AGAGACGCTT TAAGGAGCTG GAAGACATGC TGGCACAGGC ACGGGACATT 
GTTAATGCCC GTAATGCCAC TGCTGAGGCT GTGACCACCC TGATGGGCAT GATTGAGGTT 
CTCAGAGCTC AGATTGGCGA GACCACAGAC ACACTGAACC AGCAGGAGGG AGACCTGACC 
GCCGTGCAGG ACAGTAACTA TGAAGCCAGT AATGCTTTAA GCACACTGGA GAGAGAAGCA 
AAAGAGCTCA ACCTGAACGC AGATCAGCTT AACCGTCAGC TTGACATCCT CAAGAACTCC 
AACTTCTTGG GTGCATATGA CAGCATCCGG GCCTCGTATA ATAAGTCACG TGACGCAGAG 
CACCGATCAA ACAGATCTAC CACTGACACT CCCAGCACAG TCAGCCAATC AGCAGACACC 
CGCAAGAAAA CAGAGCGGCT GATTGGCCAA AAGAGAGATG ATTTTAACCG CAAGAACGCT 
GCTAACAAGC GTACACTCAC TGACTTAAAT GCAAAAGTTC AGAATCTGGA CTTAAAGAAA 
ATCAACGAAA AGGTGTGTGG AGCTCCAGGT GATGCTCAGT GTGTTGACAG TCCTTGTGGT 
GGTGCTGGTT GTCGTGATGA TGAAGGCAAG CGTCGATGTG GAGGCCTGAA CTGTAATGGA 
GCTGTTGCAG TGGCGGACAC TGCTCTGGAT AGATCCAAAC ATGCAGAAAA AGAGCTGGAT 
AAAGCAATGG GGGTAGTGGA GGAGCTTTTT AAGCAGGTGG CGGATGCTAA GACCAAGGCA 
CAGGAAGCGA AAGACAGAGC TCAGGCAGCT CTGGAAAAAG CTAGCGATAC CAAGAACAAA 
GTGGATCATT CTAACAATGA TCTAAGGGAC CTTATCAAAC AAATACGCCA ATTCCTCATG 
CAGGAGGGTG CAGATCCGGA CAGCATTGAG GCGGTGGCCA ATCGTGTGCT GGAGCTCTCA 
ATCCCTGCCT CTCCCAAGCA GATCCGACAC CTAGCAGACG AGATTAAAGA CCGTGTCAAG 
AGTCTGTCTA ATGTGGATGC CATTCTGGAG CAAACACAAA ATGACGTCCG CAAAGCAGAG 
CAACTGTTGC TGGATGCCAA GAAAGCTAGG AATAAGGCAG AGGGGGTGAA GAACACAGCT 
GAGAGTGTGA AAAAGGCCCT GAATGATGCC AGCAGAGCCC AGGCTGCAGC AGAGAAAGCC 
ATCCAGAAAG CTAAGAATGA CATTGGCTTA ACTCAGAACC AGCTGGCACA GATCCAGTCA 
GAGACCTCCG CTAGTGAGAG AGATCTTAAT GATGCAGTGG ACAGACTGGG AGACCTGGAG 
CGCCAGATCG AGGCATTAAA GACCAAACGT GCCAACAACA GCCTGGATGC TGCACGGGCA 
GAGGAGACGG CCACCATGGC ACGGGACAAA GCCAACGAAG CCAAAGAGAT CCTGGATGGT 
GAGCTGTCCG ATAAGTACCG TACTGTTCAG GGTCTGATGG ACAATAAGGC GAAAACCATG 
CAGGACGCCA AGCACAAAGC AGAGCAATTG AGAGATGAAG CCAAGGGGCT GCTGAAAGAT 
GCACAGGATA AACTGCAGAG GCTGGCAGAG CTTGAAAAGG ACTATGAAGA AAACCAGAAG 
GTATTGGAAG GCAAAGCCAG GCAGCTGGAT GGCCTGGAAG ATAAAATGAA GGCGATCCTG 
AACGCCATCA ACAGGCAGAT TCAGATCTAC AACACCTGTC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209014.1
CDS	1306..6690
Translation	MRALLLLSICVLSAVSAQEPDAAHGCTHGSCYPATGDLLVGREANLKASSTCGLRRREPYCIVSHLQEEKKCFQCDSRRPYDPQSNPVSHRIENVITTFKPHRKKSWWQSENGKSDVYLQLDLEAEFHFTHLIMTFKTFRPAAMLIERSADFGRSWQVYRYFANDCESIFPGISQDSLRKVDDVICESRYSDIEPSTEGEVIFRVLDPAIRIEDPYSPRIQNQLKITNLRLNLTKLHTLGDNLLDSRPEIKEKYYYAMYELVVRGNCFCYGHASECAPIEGIRDDIEGMVHGRCVCKHNTKGLNCEQCDDFYNDLPWRPAEGRNTNACKKCNCNGHSNQCHFDMAVYLATGNISGGVCDNCLHNTMGSNCESCKPFYYQDPTRDIRDPGVCVACDCDPDGSKNGGVCDGHDDPSLGMIAGQCRCKDNVEGSRCDKCKPGFFGLSASDPRGCQPCKCDPRGTVSGSSQCDPVSGDCFCKRLVTGHSCNQCLPEHWALSHDTSGCRGCDCDVGGATENQCSMETGQCQCRSHMIGRQCNQVESGYFFMALDHYIYEAETAKLGQGSVFEEREYQPGRPISWTGTGFARAPEGGTLEFAINNIPYSMEYDVLIRYELQMPRDWEEVRVVVIRPGPIPTSSPCGNTIPADDLLTVSLSSGARFVVLPQPVCLESGVSYKLRVELIRYADRNSIITSTNAFVLVDSIALLPRYLSLEMFTAGDPASVARKQSFERYRCHETAKSVSRPQMSEVCAKLITSMSALINDGALACECDPQGSLSSECDVRGGQCRCRPNISGRRCEKCAPGAYGFGPSGCKLCECSLEGSQSRFCDLYSGQCPCRPGAYGQRCDGCQAGHWGFPNCRPCQCNGHADECHQRTGACLNCRSNTAGDKCERCANGYYGNPVLGLGSQCRPCPCPEGPNSGRHFAASCYQDNRSQQVVCNCNQGYTGTVKRSAGAVLNRPRCDECAPGYFGDPSKPGGQCQPCRCSNNIDLSDPESCDKRTGQCLKCLYNTEGPDCSVCRSGYYGDASRRNCRKCTCNFLGTERSQCLSRDDCVCQRATGQCQCLPNVIGQTCDHCAPNSWNLASGQGCEPCACDPNNAYTSACNEFTGQCQCRVGFGGKTCTDCQENHWGDPRVLCRACDCDPRGIESSQCNRMTGHCVCQQGVSGVRCDQCARGFSGTFPDCKPCHQCFGDWDRIVQDLATRTKALTDRAKELQTTGLTRAFERRFKELEDMLAQARDIVNARNATAEAVTTLMGMIEVLRAQIGETTDTLNQQEGDLTAVQDSNYEASNALSTLEREAKELNLNADQLNRQLDILKNSNFLGAYDSIRASYNKSRDAEHRSNRSTTDTPSTVSQSADTRKKTERLIGQKRDDFNRKNAANKRTLTDLNAKVQNLDLKKINEKVCGAPGDAQCVDSPCGGAGCRDDEGKRRCGGLNCNGAVAVADTALDRSKHAEKELDKAMGVVEELFKQVADAKTKAQEAKDRAQAALEKASDTKNKVDHSNNDLRDLIKQIRQFLMQEGADPDSIEAVANRVLELSIPASPKQIRHLADEIKDRVKSLSNVDAILEQTQNDVRKAEQLLLDAKKARNKAEGVKNTAESVKKALNDASRAQAAAEKAIQKAKNDIGLTQNQLAQIQSETSASERDLNDAVDRLGDLERQIEALKTKRANNSLDAARAEETATMARDKANEAKEILDGELSDKYRTVQGLMDNKAKTMQDAKHKAEQLRDEAKGLLKDAQDKLQRLAELEKDYEENQKVLEGKARQLDGLEDKMKAILNAINRQIQIYNTCQ

Target ORF information:

RefSeq Version	XM_017353525.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio laminin, beta 2 (laminin S) (lamb2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353525.2

ORF Insert Sequence:

ATGCGCGCGC TTCTGTTGCT GTCCATCTGT GTCCTGTCAG CGGTCAGTGC ACAGGAGCCT 
GATGCTGCTC ACGGCTGCAC TCATGGAAGC TGCTATCCTG CAACCGGTGA CCTCTTGGTG 
GGTCGAGAAG CCAACCTGAA AGCATCATCC ACCTGTGGCC TGCGGAGGAG AGAACCCTAC 
TGCATTGTCA GCCATCTGCA GGAGGAGAAG AAGTGTTTCC AGTGTGATTC ACGGCGGCCT 
TATGATCCTC AGTCCAACCC CGTAAGCCAC CGCATCGAGA ACGTCATCAC CACCTTCAAA 
CCACATCGCA AGAAGTCCTG GTGGCAGTCG GAAAACGGGA AGTCTGATGT TTACCTTCAG 
CTGGATCTGG AGGCTGAGTT TCACTTCACT CATCTCATCA TGACTTTTAA GACTTTCCGT 
CCAGCAGCCA TGTTGATTGA GAGATCTGCA GATTTCGGCC GAAGCTGGCA GGTGTATCGC 
TACTTTGCTA ACGACTGCGA GTCCATCTTT CCAGGAATTT CCCAAGATTC TCTTCGAAAG 
GTTGATGATG TCATCTGCGA GTCCAGATAT TCTGATATCG AGCCGTCGAC AGAAGGAGAG 
GTGATTTTTC GTGTTCTTGA CCCTGCTATC AGGATCGAGG ACCCTTACAG CCCAAGAATT 
CAGAACCAGC TGAAGATCAC AAATCTTCGC CTGAATCTTA CCAAGCTGCA CACTCTGGGT 
GACAATCTGC TGGACTCACG GCCTGAGATC AAGGAGAAAT ACTACTATGC CATGTATGAG 
CTGGTCGTCA GGGGCAACTG CTTCTGCTAT GGCCACGCCT CTGAGTGTGC GCCAATTGAG 
GGCATCAGGG ATGACATTGA AGGCATGGTT CACGGGAGGT GTGTGTGCAA ACACAACACT 
AAAGGACTGA ACTGTGAACA GTGTGACGAC TTCTACAATG ATCTGCCCTG GAGACCAGCA 
GAAGGCCGGA ACACTAATGC CTGCAAGAAA TGCAACTGTA ATGGTCACTC CAACCAGTGT 
CATTTTGACA TGGCGGTGTA TTTGGCAACG GGGAACATCA GTGGAGGTGT GTGTGACAAC 
TGCCTTCACA ACACCATGGG CAGCAACTGT GAGAGCTGTA AACCTTTCTA CTACCAGGAT 
CCAACTAGAG ATATTAGAGA CCCTGGAGTC TGTGTAGCTT GCGATTGTGA CCCAGATGGC 
TCTAAGAACG GTGGTGTTTG TGACGGCCAT GATGACCCTT CTCTGGGCAT GATTGCTGGA 
CAGTGCCGCT GTAAAGACAA TGTTGAAGGT TCACGCTGTG ATAAATGCAA GCCTGGTTTC 
TTTGGCCTGA GTGCTAGTGA TCCACGTGGT TGTCAACCAT GTAAATGTGA CCCCAGGGGT 
ACAGTTTCCG GCAGCTCCCA ATGTGACCCT GTGAGCGGAG ACTGCTTCTG TAAACGCCTG 
GTCACTGGAC ACAGCTGCAA CCAATGTCTG CCTGAGCACT GGGCCTTGAG TCATGATACC 
AGTGGCTGCA GAGGGTGTGA CTGTGATGTG GGCGGAGCTA CAGAAAACCA ATGCTCAATG 
GAAACAGGGC AGTGTCAGTG TCGAAGCCAC ATGATTGGTC GCCAGTGCAA CCAGGTGGAG 
TCTGGGTACT TTTTCATGGC ACTTGACCAC TACATCTATG AAGCTGAAAC TGCCAAACTG 
GGTCAAGGCT CTGTTTTTGA GGAGAGAGAG TACCAGCCGG GTCGCCCCAT CTCATGGACA 
GGCACAGGCT TTGCACGAGC TCCTGAGGGC GGGACGCTGG AATTCGCAAT TAACAACATC 
CCATACTCTA TGGAATATGA CGTCCTGATT CGCTATGAGC TTCAGATGCC ACGTGACTGG 
GAAGAGGTGC GTGTTGTAGT GATTAGACCT GGTCCTATAC CTACCAGCAG TCCATGTGGA 
AACACCATCC CTGCAGATGA TCTGCTAACG GTGTCGCTTT CCTCTGGTGC CAGGTTTGTG 
GTTCTGCCTC AGCCAGTGTG TTTGGAGAGT GGTGTGAGTT ACAAACTGCG CGTGGAGCTC 
ATCCGTTACG CTGACAGGAA CAGCATCATT ACCTCCACCA ATGCCTTTGT GCTTGTTGAC 
TCTATCGCAC TTCTCCCCCG TTACTTGTCT CTGGAGATGT TTACTGCAGG AGACCCGGCC 
TCTGTCGCAC GCAAGCAGAG TTTTGAGCGT TACCGCTGCC ACGAAACTGC CAAGAGTGTG 
TCCCGTCCGC AGATGAGTGA AGTGTGTGCC AAACTCATCA CCAGCATGTC TGCCCTCATT 
AATGACGGCG CGCTGGCGTG TGAGTGTGAT CCTCAAGGTT CGCTCAGCTC TGAGTGTGAT 
GTCCGTGGCG GTCAGTGCCG CTGCAGGCCC AACATATCTG GCCGGCGCTG TGAGAAATGT 
GCACCAGGAG CTTACGGCTT TGGCCCATCT GGATGCAAAC TTTGTGAATG CAGTTTAGAG 
GGCTCTCAGA GCCGGTTCTG TGACCTGTAC TCAGGACAGT GTCCATGCCG ACCTGGAGCA 
TATGGTCAGC GCTGTGATGG TTGTCAGGCA GGACACTGGG GTTTCCCAAA CTGCAGGCCA 
TGTCAATGTA ACGGCCATGC TGACGAGTGC CATCAGAGGA CTGGAGCCTG TCTCAACTGC 
AGAAGTAATA CTGCAGGAGA CAAATGTGAA AGGTGTGCTA ATGGCTACTA TGGTAACCCA 
GTCCTTGGTT TAGGGAGTCA GTGTCGGCCC TGCCCCTGTC CTGAGGGCCC CAACAGCGGA 
CGCCACTTTG CTGCCTCTTG TTACCAAGAC AACCGAAGCC AACAGGTGGT CTGCAACTGC 
AACCAGGGTT ACACAGGGAC TGTAAAGCGC TCTGCAGGGG CAGTCCTCAA TCGTCCACGT 
TGTGATGAAT GTGCTCCTGG CTATTTTGGT GACCCGTCTA AGCCCGGTGG GCAGTGCCAG 
CCATGTCGTT GCAGTAATAA CATCGACCTG TCAGATCCTG AGTCTTGTGA CAAGCGCACT 
GGCCAGTGTC TGAAGTGCCT GTACAACACT GAAGGGCCAG ACTGTAGTGT GTGCAGGAGC 
GGATACTATG GAGACGCTTC TCGACGCAAC TGCCGGAAGT GCACCTGTAA CTTCCTGGGC 
ACAGAGCGCA GTCAGTGTCT GTCTCGAGAT GACTGTGTGT GTCAGCGTGC CACTGGTCAG 
TGTCAGTGTC TGCCTAATGT TATTGGACAA ACCTGTGATC ACTGCGCTCC AAACTCCTGG 
AACCTGGCCA GCGGGCAAGG CTGTGAGCCC TGCGCATGTG ACCCTAACAA TGCCTACACA 
TCTGCCTGTA ATGAGTTTAC AGGCCAGTGC CAGTGCCGTG TTGGGTTTGG AGGAAAGACC 
TGTACAGACT GTCAGGAGAA CCACTGGGGT GACCCAAGAG TGCTATGCCG AGCATGTGAC 
TGTGACCCCC GTGGCATCGA GTCATCCCAA TGCAACCGTA TGACGGGTCA CTGCGTGTGC 
CAGCAGGGTG TTTCTGGTGT CCGCTGTGAT CAGTGTGCCC GTGGCTTCTC CGGGACCTTC 
CCTGACTGTA AGCCTTGCCA CCAGTGTTTT GGGGACTGGG ACCGTATCGT TCAGGATTTG 
GCCACCAGAA CTAAAGCCCT CACAGATCGA GCCAAGGAGC TTCAGACCAC CGGCCTGACT 
CGAGCATTCG AGAGACGCTT TAAGGAGCTG GAAGACATGC TGGCACAGGC ACGGGACATT 
GTTAATGCCC GTAATGCCAC TGCTGAGGCT GTGACCACCC TGATGGGCAT GATTGAGGTT 
CTCAGAGCTC AGATTGGCGA GACCACAGAC ACACTGAACC AGCAGGAGGG AGACCTGACC 
GCCGTGCAGG ACAGTAACTA TGAAGCCAGT AATGCTTTAA GCACACTGGA GAGAGAAGCA 
AAAGAGCTCA ACCTGAACGC AGATCAGCTT AACCGTCAGC TTGACATCCT CAAGAACTCC 
AACTTCTTGG GTGCATATGA CAGCATCCGG GCCTCGTATA ATAAGTCACG TGACGCAGAG 
CACCGATCAA ACAGATCTAC CACTGACACT CCCAGCACAG TCAGCCAATC AGCAGACACC 
CGCAAGAAAA CAGAGCGGCT GATTGGCCAA AAGAGAGATG ATTTTAACCG CAAGAACGCT 
GCTAACAAGC GTACACTCAC TGACTTAAAT GCAAAAGTTC AGAATCTGGA CTTAAAGAAA 
ATCAACGAAA AGGTGTGTGG AGCTCCAGGT GATGCTCAGT GTGTTGACAG TCCTTGTGGT 
GGTGCTGGTT GTCGTGATGA TGAAGGCAAG CGTCGATGTG GAGGCCTGAA CTGTAATGGA 
GCTGTTGCAG TGGCGGACAC TGCTCTGGAT AGATCCAAAC ATGCAGAAAA AGAGCTGGAT 
AAAGCAATGG GGGTAGTGGA GGAGCTTTTT AAGCAGGTGG CGGATGCTAA GACCAAGGCA 
CAGGAAGCGA AAGACAGAGC TCAGGCAGCT CTGGAAAAAG CTAGCGATAC CAAGAACAAA 
GTGGATCATT CTAACAATGA TCTAAGGGAC CTTATCAAAC AAATACGCCA ATTCCTCATG 
CAGGAGGGTG CAGATCCGGA CAGCATTGAG GCGGTGGCCA ATCGTGTGCT GGAGCTCTCA 
ATCCCTGCCT CTCCCAAGCA GATCCGACAC CTAGCAGACG AGATTAAAGA CCGTGTCAAG 
AGTCTGTCTA ATGTGGATGC CATTCTGGAG CAAACACAAA ATGACGTCCG CAAAGCAGAG 
CAACTGTTGC TGGATGCCAA GAAAGCTAGG AATAAGGCAG AGGGGGTGAA GAACACAGCT 
GAGAGTGTGA AAAAGGCCCT GAATGATGCC AGCAGAGCCC AGGCTGCAGC AGAGAAAGCC 
ATCCAGAAAG CTAAGAATGA CATTGGCTTA ACTCAGAACC AGCTGGCACA GATCCAGTCA 
GAGACCTCCG CTAGTGAGAG AGATCTTAAT GATGCAGTGG ACAGACTGGG AGACCTGGAG 
CGCCAGATCG AGGCATTAAA GACCAAACGT GCCAACAACA GCCTGGATGC TGCACGGGCA 
GAGGAGACGG CCACCATGGC ACGGGACAAA GCCAACGAAG CCAAAGAGAT CCTGGATGGT 
GAGCTGTCCG ATAAGTACCG TACTGTTCAG GGTCTGATGG ACAATAAGGC GAAAACCATG 
CAGGACGCCA AGCACAAAGC AGAGCAATTG AGAGATGAAG CCAAGGGGCT GCTGAAAGAT 
GCACAGGATA AACTGCAGAG GCTGGCAGAG CTTGAAAAGG ACTATGAAGA AAACCAGAAG 
GTATTGGAAG GCAAAGCCAG GCAGCTGGAT GGCCTGGAAG ATAAAATGAA GGCGATCCTG 
AACGCCATCA ACAGGCAGAT TCAGATCTAC AACACCTGTC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LAMB2 ( XM_005162046.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005162103.1 Danio rerio laminin, beta 2 (laminin S) (lamb2), transcript variant X3, mRNA.

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CloneID	ODa27565
Clone ID Related Accession (Same CDS sequence)	XM_017353525.1 , XM_005162046.2 , XM_017353525.2 , XM_005162046.3 , XM_005162045.3 , XM_005162045.2
Accession Version	XM_005162046.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	laminin subunit beta-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005162046.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGCGC TTCTGTTGCT GTCCATCTGT GTCCTGTCAG CGGTCAGTGC ACAGGAGCCT 
GATGCTGCTC ACGGCTGCAC TCATGGAAGC TGCTATCCTG CAACCGGTGA CCTCTTGGTG 
GGTCGAGAAG CCAACCTGAA AGCATCATCC ACCTGTGGCC TGCGGAGGAG AGAACCCTAC 
TGCATTGTCA GCCATCTGCA GGAGGAGAAG AAGTGTTTCC AGTGTGATTC ACGGCGGCCT 
TATGATCCTC AGTCCAACCC CGTAAGCCAC CGCATCGAGA ACGTCATCAC CACCTTCAAA 
CCACATCGCA AGAAGTCCTG GTGGCAGTCG GAAAACGGGA AGTCTGATGT TTACCTTCAG 
CTGGATCTGG AGGCTGAGTT TCACTTCACT CATCTCATCA TGACTTTTAA GACTTTCCGT 
CCAGCAGCCA TGTTGATTGA GAGATCTGCA GATTTCGGCC GAAGCTGGCA GGTGTATCGC 
TACTTTGCTA ACGACTGCGA GTCCATCTTT CCAGGAATTT CCCAAGATTC TCTTCGAAAG 
GTTGATGATG TCATCTGCGA GTCCAGATAT TCTGATATCG AGCCGTCGAC AGAAGGAGAG 
GTGATTTTTC GTGTTCTTGA CCCTGCTATC AGGATCGAGG ACCCTTACAG CCCAAGAATT 
CAGAACCAGC TGAAGATCAC AAATCTTCGC CTGAATCTTA CCAAGCTGCA CACTCTGGGT 
GACAATCTGC TGGACTCACG GCCTGAGATC AAGGAGAAAT ACTACTATGC CATGTATGAG 
CTGGTCGTCA GGGGCAACTG CTTCTGCTAT GGCCACGCCT CTGAGTGTGC GCCAATTGAG 
GGCATCAGGG ATGACATTGA AGGCATGGTT CACGGGAGGT GTGTGTGCAA ACACAACACT 
AAAGGACTGA ACTGTGAACA GTGTGACGAC TTCTACAATG ATCTGCCCTG GAGACCAGCA 
GAAGGCCGGA ACACTAATGC CTGCAAGAAA TGCAACTGTA ATGGTCACTC CAACCAGTGT 
CATTTTGACA TGGCGGTGTA TTTGGCAACG GGGAACATCA GTGGAGGTGT GTGTGACAAC 
TGCCTTCACA ACACCATGGG CAGCAACTGT GAGAGCTGTA AACCTTTCTA CTACCAGGAT 
CCAACTAGAG ATATTAGAGA CCCTGGAGTC TGTGTAGCTT GCGATTGTGA CCCAGATGGC 
TCTAAGAACG GTGGTGTTTG TGACGGCCAT GATGACCCTT CTCTGGGCAT GATTGCTGGA 
CAGTGCCGCT GTAAAGACAA TGTTGAAGGT TCACGCTGTG ATAAATGCAA GCCTGGTTTC 
TTTGGCCTGA GTGCTAGTGA TCCACGTGGT TGTCAACCAT GTAAATGTGA CCCCAGGGGT 
ACAGTTTCCG GCAGCTCCCA ATGTGACCCT GTGAGCGGAG ACTGCTTCTG TAAACGCCTG 
GTCACTGGAC ACAGCTGCAA CCAATGTCTG CCTGAGCACT GGGCCTTGAG TCATGATACC 
AGTGGCTGCA GAGGGTGTGA CTGTGATGTG GGCGGAGCTA CAGAAAACCA ATGCTCAATG 
GAAACAGGGC AGTGTCAGTG TCGAAGCCAC ATGATTGGTC GCCAGTGCAA CCAGGTGGAG 
TCTGGGTACT TTTTCATGGC ACTTGACCAC TACATCTATG AAGCTGAAAC TGCCAAACTG 
GGTCAAGGCT CTGTTTTTGA GGAGAGAGAG TACCAGCCGG GTCGCCCCAT CTCATGGACA 
GGCACAGGCT TTGCACGAGC TCCTGAGGGC GGGACGCTGG AATTCGCAAT TAACAACATC 
CCATACTCTA TGGAATATGA CGTCCTGATT CGCTATGAGC TTCAGATGCC ACGTGACTGG 
GAAGAGGTGC GTGTTGTAGT GATTAGACCT GGTCCTATAC CTACCAGCAG TCCATGTGGA 
AACACCATCC CTGCAGATGA TCTGCTAACG GTGTCGCTTT CCTCTGGTGC CAGGTTTGTG 
GTTCTGCCTC AGCCAGTGTG TTTGGAGAGT GGTGTGAGTT ACAAACTGCG CGTGGAGCTC 
ATCCGTTACG CTGACAGGAA CAGCATCATT ACCTCCACCA ATGCCTTTGT GCTTGTTGAC 
TCTATCGCAC TTCTCCCCCG TTACTTGTCT CTGGAGATGT TTACTGCAGG AGACCCGGCC 
TCTGTCGCAC GCAAGCAGAG TTTTGAGCGT TACCGCTGCC ACGAAACTGC CAAGAGTGTG 
TCCCGTCCGC AGATGAGTGA AGTGTGTGCC AAACTCATCA CCAGCATGTC TGCCCTCATT 
AATGACGGCG CGCTGGCGTG TGAGTGTGAT CCTCAAGGTT CGCTCAGCTC TGAGTGTGAT 
GTCCGTGGCG GTCAGTGCCG CTGCAGGCCC AACATATCTG GCCGGCGCTG TGAGAAATGT 
GCACCAGGAG CTTACGGCTT TGGCCCATCT GGATGCAAAC TTTGTGAATG CAGTTTAGAG 
GGCTCTCAGA GCCGGTTCTG TGACCTGTAC TCAGGACAGT GTCCATGCCG ACCTGGAGCA 
TATGGTCAGC GCTGTGATGG TTGTCAGGCA GGACACTGGG GTTTCCCAAA CTGCAGGCCA 
TGTCAATGTA ACGGCCATGC TGACGAGTGC CATCAGAGGA CTGGAGCCTG TCTCAACTGC 
AGAAGTAATA CTGCAGGAGA CAAATGTGAA AGGTGTGCTA ATGGCTACTA TGGTAACCCA 
GTCCTTGGTT TAGGGAGTCA GTGTCGGCCC TGCCCCTGTC CTGAGGGCCC CAACAGCGGA 
CGCCACTTTG CTGCCTCTTG TTACCAAGAC AACCGAAGCC AACAGGTGGT CTGCAACTGC 
AACCAGGGTT ACACAGGGAC TGTAAAGCGC TCTGCAGGGG CAGTCCTCAA TCGTCCACGT 
TGTGATGAAT GTGCTCCTGG CTATTTTGGT GACCCGTCTA AGCCCGGTGG GCAGTGCCAG 
CCATGTCGTT GCAGTAATAA CATCGACCTG TCAGATCCTG AGTCTTGTGA CAAGCGCACT 
GGCCAGTGTC TGAAGTGCCT GTACAACACT GAAGGGCCAG ACTGTAGTGT GTGCAGGAGC 
GGATACTATG GAGACGCTTC TCGACGCAAC TGCCGGAAGT GCACCTGTAA CTTCCTGGGC 
ACAGAGCGCA GTCAGTGTCT GTCTCGAGAT GACTGTGTGT GTCAGCGTGC CACTGGTCAG 
TGTCAGTGTC TGCCTAATGT TATTGGACAA ACCTGTGATC ACTGCGCTCC AAACTCCTGG 
AACCTGGCCA GCGGGCAAGG CTGTGAGCCC TGCGCATGTG ACCCTAACAA TGCCTACACA 
TCTGCCTGTA ATGAGTTTAC AGGCCAGTGC CAGTGCCGTG TTGGGTTTGG AGGAAAGACC 
TGTACAGACT GTCAGGAGAA CCACTGGGGT GACCCAAGAG TGCTATGCCG AGCATGTGAC 
TGTGACCCCC GTGGCATCGA GTCATCCCAA TGCAACCGTA TGACGGGTCA CTGCGTGTGC 
CAGCAGGGTG TTTCTGGTGT CCGCTGTGAT CAGTGTGCCC GTGGCTTCTC CGGGACCTTC 
CCTGACTGTA AGCCTTGCCA CCAGTGTTTT GGGGACTGGG ACCGTATCGT TCAGGATTTG 
GCCACCAGAA CTAAAGCCCT CACAGATCGA GCCAAGGAGC TTCAGACCAC CGGCCTGACT 
CGAGCATTCG AGAGACGCTT TAAGGAGCTG GAAGACATGC TGGCACAGGC ACGGGACATT 
GTTAATGCCC GTAATGCCAC TGCTGAGGCT GTGACCACCC TGATGGGCAT GATTGAGGTT 
CTCAGAGCTC AGATTGGCGA GACCACAGAC ACACTGAACC AGCAGGAGGG AGACCTGACC 
GCCGTGCAGG ACAGTAACTA TGAAGCCAGT AATGCTTTAA GCACACTGGA GAGAGAAGCA 
AAAGAGCTCA ACCTGAACGC AGATCAGCTT AACCGTCAGC TTGACATCCT CAAGAACTCC 
AACTTCTTGG GTGCATATGA CAGCATCCGG GCCTCGTATA ATAAGTCACG TGACGCAGAG 
CACCGATCAA ACAGATCTAC CACTGACACT CCCAGCACAG TCAGCCAATC AGCAGACACC 
CGCAAGAAAA CAGAGCGGCT GATTGGCCAA AAGAGAGATG ATTTTAACCG CAAGAACGCT 
GCTAACAAGC GTACACTCAC TGACTTAAAT GCAAAAGTTC AGAATCTGGA CTTAAAGAAA 
ATCAACGAAA AGGTGTGTGG AGCTCCAGGT GATGCTCAGT GTGTTGACAG TCCTTGTGGT 
GGTGCTGGTT GTCGTGATGA TGAAGGCAAG CGTCGATGTG GAGGCCTGAA CTGTAATGGA 
GCTGTTGCAG TGGCGGACAC TGCTCTGGAT AGATCCAAAC ATGCAGAAAA AGAGCTGGAT 
AAAGCAATGG GGGTAGTGGA GGAGCTTTTT AAGCAGGTGG CGGATGCTAA GACCAAGGCA 
CAGGAAGCGA AAGACAGAGC TCAGGCAGCT CTGGAAAAAG CTAGCGATAC CAAGAACAAA 
GTGGATCATT CTAACAATGA TCTAAGGGAC CTTATCAAAC AAATACGCCA ATTCCTCATG 
CAGGAGGGTG CAGATCCGGA CAGCATTGAG GCGGTGGCCA ATCGTGTGCT GGAGCTCTCA 
ATCCCTGCCT CTCCCAAGCA GATCCGACAC CTAGCAGACG AGATTAAAGA CCGTGTCAAG 
AGTCTGTCTA ATGTGGATGC CATTCTGGAG CAAACACAAA ATGACGTCCG CAAAGCAGAG 
CAACTGTTGC TGGATGCCAA GAAAGCTAGG AATAAGGCAG AGGGGGTGAA GAACACAGCT 
GAGAGTGTGA AAAAGGCCCT GAATGATGCC AGCAGAGCCC AGGCTGCAGC AGAGAAAGCC 
ATCCAGAAAG CTAAGAATGA CATTGGCTTA ACTCAGAACC AGCTGGCACA GATCCAGTCA 
GAGACCTCCG CTAGTGAGAG AGATCTTAAT GATGCAGTGG ACAGACTGGG AGACCTGGAG 
CGCCAGATCG AGGCATTAAA GACCAAACGT GCCAACAACA GCCTGGATGC TGCACGGGCA 
GAGGAGACGG CCACCATGGC ACGGGACAAA GCCAACGAAG CCAAAGAGAT CCTGGATGGT 
GAGCTGTCCG ATAAGTACCG TACTGTTCAG GGTCTGATGG ACAATAAGGC GAAAACCATG 
CAGGACGCCA AGCACAAAGC AGAGCAATTG AGAGATGAAG CCAAGGGGCT GCTGAAAGAT 
GCACAGGATA AACTGCAGAG GCTGGCAGAG CTTGAAAAGG ACTATGAAGA AAACCAGAAG 
GTATTGGAAG GCAAAGCCAG GCAGCTGGAT GGCCTGGAAG ATAAAATGAA GGCGATCCTG 
AACGCCATCA ACAGGCAGAT TCAGATCTAC AACACCTGTC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162103.1
CDS	1188..6572
Translation	MRALLLLSICVLSAVSAQEPDAAHGCTHGSCYPATGDLLVGREANLKASSTCGLRRREPYCIVSHLQEEKKCFQCDSRRPYDPQSNPVSHRIENVITTFKPHRKKSWWQSENGKSDVYLQLDLEAEFHFTHLIMTFKTFRPAAMLIERSADFGRSWQVYRYFANDCESIFPGISQDSLRKVDDVICESRYSDIEPSTEGEVIFRVLDPAIRIEDPYSPRIQNQLKITNLRLNLTKLHTLGDNLLDSRPEIKEKYYYAMYELVVRGNCFCYGHASECAPIEGIRDDIEGMVHGRCVCKHNTKGLNCEQCDDFYNDLPWRPAEGRNTNACKKCNCNGHSNQCHFDMAVYLATGNISGGVCDNCLHNTMGSNCESCKPFYYQDPTRDIRDPGVCVACDCDPDGSKNGGVCDGHDDPSLGMIAGQCRCKDNVEGSRCDKCKPGFFGLSASDPRGCQPCKCDPRGTVSGSSQCDPVSGDCFCKRLVTGHSCNQCLPEHWALSHDTSGCRGCDCDVGGATENQCSMETGQCQCRSHMIGRQCNQVESGYFFMALDHYIYEAETAKLGQGSVFEEREYQPGRPISWTGTGFARAPEGGTLEFAINNIPYSMEYDVLIRYELQMPRDWEEVRVVVIRPGPIPTSSPCGNTIPADDLLTVSLSSGARFVVLPQPVCLESGVSYKLRVELIRYADRNSIITSTNAFVLVDSIALLPRYLSLEMFTAGDPASVARKQSFERYRCHETAKSVSRPQMSEVCAKLITSMSALINDGALACECDPQGSLSSECDVRGGQCRCRPNISGRRCEKCAPGAYGFGPSGCKLCECSLEGSQSRFCDLYSGQCPCRPGAYGQRCDGCQAGHWGFPNCRPCQCNGHADECHQRTGACLNCRSNTAGDKCERCANGYYGNPVLGLGSQCRPCPCPEGPNSGRHFAASCYQDNRSQQVVCNCNQGYTGTVKRSAGAVLNRPRCDECAPGYFGDPSKPGGQCQPCRCSNNIDLSDPESCDKRTGQCLKCLYNTEGPDCSVCRSGYYGDASRRNCRKCTCNFLGTERSQCLSRDDCVCQRATGQCQCLPNVIGQTCDHCAPNSWNLASGQGCEPCACDPNNAYTSACNEFTGQCQCRVGFGGKTCTDCQENHWGDPRVLCRACDCDPRGIESSQCNRMTGHCVCQQGVSGVRCDQCARGFSGTFPDCKPCHQCFGDWDRIVQDLATRTKALTDRAKELQTTGLTRAFERRFKELEDMLAQARDIVNARNATAEAVTTLMGMIEVLRAQIGETTDTLNQQEGDLTAVQDSNYEASNALSTLEREAKELNLNADQLNRQLDILKNSNFLGAYDSIRASYNKSRDAEHRSNRSTTDTPSTVSQSADTRKKTERLIGQKRDDFNRKNAANKRTLTDLNAKVQNLDLKKINEKVCGAPGDAQCVDSPCGGAGCRDDEGKRRCGGLNCNGAVAVADTALDRSKHAEKELDKAMGVVEELFKQVADAKTKAQEAKDRAQAALEKASDTKNKVDHSNNDLRDLIKQIRQFLMQEGADPDSIEAVANRVLELSIPASPKQIRHLADEIKDRVKSLSNVDAILEQTQNDVRKAEQLLLDAKKARNKAEGVKNTAESVKKALNDASRAQAAAEKAIQKAKNDIGLTQNQLAQIQSETSASERDLNDAVDRLGDLERQIEALKTKRANNSLDAARAEETATMARDKANEAKEILDGELSDKYRTVQGLMDNKAKTMQDAKHKAEQLRDEAKGLLKDAQDKLQRLAELEKDYEENQKVLEGKARQLDGLEDKMKAILNAINRQIQIYNTCQ

Target ORF information:

RefSeq Version	XM_005162046.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio laminin, beta 2 (laminin S) (lamb2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162046.3

ORF Insert Sequence:

ATGCGCGCGC TTCTGTTGCT GTCCATCTGT GTCCTGTCAG CGGTCAGTGC ACAGGAGCCT 
GATGCTGCTC ACGGCTGCAC TCATGGAAGC TGCTATCCTG CAACCGGTGA CCTCTTGGTG 
GGTCGAGAAG CCAACCTGAA AGCATCATCC ACCTGTGGCC TGCGGAGGAG AGAACCCTAC 
TGCATTGTCA GCCATCTGCA GGAGGAGAAG AAGTGTTTCC AGTGTGATTC ACGGCGGCCT 
TATGATCCTC AGTCCAACCC CGTAAGCCAC CGCATCGAGA ACGTCATCAC CACCTTCAAA 
CCACATCGCA AGAAGTCCTG GTGGCAGTCG GAAAACGGGA AGTCTGATGT TTACCTTCAG 
CTGGATCTGG AGGCTGAGTT TCACTTCACT CATCTCATCA TGACTTTTAA GACTTTCCGT 
CCAGCAGCCA TGTTGATTGA GAGATCTGCA GATTTCGGCC GAAGCTGGCA GGTGTATCGC 
TACTTTGCTA ACGACTGCGA GTCCATCTTT CCAGGAATTT CCCAAGATTC TCTTCGAAAG 
GTTGATGATG TCATCTGCGA GTCCAGATAT TCTGATATCG AGCCGTCGAC AGAAGGAGAG 
GTGATTTTTC GTGTTCTTGA CCCTGCTATC AGGATCGAGG ACCCTTACAG CCCAAGAATT 
CAGAACCAGC TGAAGATCAC AAATCTTCGC CTGAATCTTA CCAAGCTGCA CACTCTGGGT 
GACAATCTGC TGGACTCACG GCCTGAGATC AAGGAGAAAT ACTACTATGC CATGTATGAG 
CTGGTCGTCA GGGGCAACTG CTTCTGCTAT GGCCACGCCT CTGAGTGTGC GCCAATTGAG 
GGCATCAGGG ATGACATTGA AGGCATGGTT CACGGGAGGT GTGTGTGCAA ACACAACACT 
AAAGGACTGA ACTGTGAACA GTGTGACGAC TTCTACAATG ATCTGCCCTG GAGACCAGCA 
GAAGGCCGGA ACACTAATGC CTGCAAGAAA TGCAACTGTA ATGGTCACTC CAACCAGTGT 
CATTTTGACA TGGCGGTGTA TTTGGCAACG GGGAACATCA GTGGAGGTGT GTGTGACAAC 
TGCCTTCACA ACACCATGGG CAGCAACTGT GAGAGCTGTA AACCTTTCTA CTACCAGGAT 
CCAACTAGAG ATATTAGAGA CCCTGGAGTC TGTGTAGCTT GCGATTGTGA CCCAGATGGC 
TCTAAGAACG GTGGTGTTTG TGACGGCCAT GATGACCCTT CTCTGGGCAT GATTGCTGGA 
CAGTGCCGCT GTAAAGACAA TGTTGAAGGT TCACGCTGTG ATAAATGCAA GCCTGGTTTC 
TTTGGCCTGA GTGCTAGTGA TCCACGTGGT TGTCAACCAT GTAAATGTGA CCCCAGGGGT 
ACAGTTTCCG GCAGCTCCCA ATGTGACCCT GTGAGCGGAG ACTGCTTCTG TAAACGCCTG 
GTCACTGGAC ACAGCTGCAA CCAATGTCTG CCTGAGCACT GGGCCTTGAG TCATGATACC 
AGTGGCTGCA GAGGGTGTGA CTGTGATGTG GGCGGAGCTA CAGAAAACCA ATGCTCAATG 
GAAACAGGGC AGTGTCAGTG TCGAAGCCAC ATGATTGGTC GCCAGTGCAA CCAGGTGGAG 
TCTGGGTACT TTTTCATGGC ACTTGACCAC TACATCTATG AAGCTGAAAC TGCCAAACTG 
GGTCAAGGCT CTGTTTTTGA GGAGAGAGAG TACCAGCCGG GTCGCCCCAT CTCATGGACA 
GGCACAGGCT TTGCACGAGC TCCTGAGGGC GGGACGCTGG AATTCGCAAT TAACAACATC 
CCATACTCTA TGGAATATGA CGTCCTGATT CGCTATGAGC TTCAGATGCC ACGTGACTGG 
GAAGAGGTGC GTGTTGTAGT GATTAGACCT GGTCCTATAC CTACCAGCAG TCCATGTGGA 
AACACCATCC CTGCAGATGA TCTGCTAACG GTGTCGCTTT CCTCTGGTGC CAGGTTTGTG 
GTTCTGCCTC AGCCAGTGTG TTTGGAGAGT GGTGTGAGTT ACAAACTGCG CGTGGAGCTC 
ATCCGTTACG CTGACAGGAA CAGCATCATT ACCTCCACCA ATGCCTTTGT GCTTGTTGAC 
TCTATCGCAC TTCTCCCCCG TTACTTGTCT CTGGAGATGT TTACTGCAGG AGACCCGGCC 
TCTGTCGCAC GCAAGCAGAG TTTTGAGCGT TACCGCTGCC ACGAAACTGC CAAGAGTGTG 
TCCCGTCCGC AGATGAGTGA AGTGTGTGCC AAACTCATCA CCAGCATGTC TGCCCTCATT 
AATGACGGCG CGCTGGCGTG TGAGTGTGAT CCTCAAGGTT CGCTCAGCTC TGAGTGTGAT 
GTCCGTGGCG GTCAGTGCCG CTGCAGGCCC AACATATCTG GCCGGCGCTG TGAGAAATGT 
GCACCAGGAG CTTACGGCTT TGGCCCATCT GGATGCAAAC TTTGTGAATG CAGTTTAGAG 
GGCTCTCAGA GCCGGTTCTG TGACCTGTAC TCAGGACAGT GTCCATGCCG ACCTGGAGCA 
TATGGTCAGC GCTGTGATGG TTGTCAGGCA GGACACTGGG GTTTCCCAAA CTGCAGGCCA 
TGTCAATGTA ACGGCCATGC TGACGAGTGC CATCAGAGGA CTGGAGCCTG TCTCAACTGC 
AGAAGTAATA CTGCAGGAGA CAAATGTGAA AGGTGTGCTA ATGGCTACTA TGGTAACCCA 
GTCCTTGGTT TAGGGAGTCA GTGTCGGCCC TGCCCCTGTC CTGAGGGCCC CAACAGCGGA 
CGCCACTTTG CTGCCTCTTG TTACCAAGAC AACCGAAGCC AACAGGTGGT CTGCAACTGC 
AACCAGGGTT ACACAGGGAC TGTAAAGCGC TCTGCAGGGG CAGTCCTCAA TCGTCCACGT 
TGTGATGAAT GTGCTCCTGG CTATTTTGGT GACCCGTCTA AGCCCGGTGG GCAGTGCCAG 
CCATGTCGTT GCAGTAATAA CATCGACCTG TCAGATCCTG AGTCTTGTGA CAAGCGCACT 
GGCCAGTGTC TGAAGTGCCT GTACAACACT GAAGGGCCAG ACTGTAGTGT GTGCAGGAGC 
GGATACTATG GAGACGCTTC TCGACGCAAC TGCCGGAAGT GCACCTGTAA CTTCCTGGGC 
ACAGAGCGCA GTCAGTGTCT GTCTCGAGAT GACTGTGTGT GTCAGCGTGC CACTGGTCAG 
TGTCAGTGTC TGCCTAATGT TATTGGACAA ACCTGTGATC ACTGCGCTCC AAACTCCTGG 
AACCTGGCCA GCGGGCAAGG CTGTGAGCCC TGCGCATGTG ACCCTAACAA TGCCTACACA 
TCTGCCTGTA ATGAGTTTAC AGGCCAGTGC CAGTGCCGTG TTGGGTTTGG AGGAAAGACC 
TGTACAGACT GTCAGGAGAA CCACTGGGGT GACCCAAGAG TGCTATGCCG AGCATGTGAC 
TGTGACCCCC GTGGCATCGA GTCATCCCAA TGCAACCGTA TGACGGGTCA CTGCGTGTGC 
CAGCAGGGTG TTTCTGGTGT CCGCTGTGAT CAGTGTGCCC GTGGCTTCTC CGGGACCTTC 
CCTGACTGTA AGCCTTGCCA CCAGTGTTTT GGGGACTGGG ACCGTATCGT TCAGGATTTG 
GCCACCAGAA CTAAAGCCCT CACAGATCGA GCCAAGGAGC TTCAGACCAC CGGCCTGACT 
CGAGCATTCG AGAGACGCTT TAAGGAGCTG GAAGACATGC TGGCACAGGC ACGGGACATT 
GTTAATGCCC GTAATGCCAC TGCTGAGGCT GTGACCACCC TGATGGGCAT GATTGAGGTT 
CTCAGAGCTC AGATTGGCGA GACCACAGAC ACACTGAACC AGCAGGAGGG AGACCTGACC 
GCCGTGCAGG ACAGTAACTA TGAAGCCAGT AATGCTTTAA GCACACTGGA GAGAGAAGCA 
AAAGAGCTCA ACCTGAACGC AGATCAGCTT AACCGTCAGC TTGACATCCT CAAGAACTCC 
AACTTCTTGG GTGCATATGA CAGCATCCGG GCCTCGTATA ATAAGTCACG TGACGCAGAG 
CACCGATCAA ACAGATCTAC CACTGACACT CCCAGCACAG TCAGCCAATC AGCAGACACC 
CGCAAGAAAA CAGAGCGGCT GATTGGCCAA AAGAGAGATG ATTTTAACCG CAAGAACGCT 
GCTAACAAGC GTACACTCAC TGACTTAAAT GCAAAAGTTC AGAATCTGGA CTTAAAGAAA 
ATCAACGAAA AGGTGTGTGG AGCTCCAGGT GATGCTCAGT GTGTTGACAG TCCTTGTGGT 
GGTGCTGGTT GTCGTGATGA TGAAGGCAAG CGTCGATGTG GAGGCCTGAA CTGTAATGGA 
GCTGTTGCAG TGGCGGACAC TGCTCTGGAT AGATCCAAAC ATGCAGAAAA AGAGCTGGAT 
AAAGCAATGG GGGTAGTGGA GGAGCTTTTT AAGCAGGTGG CGGATGCTAA GACCAAGGCA 
CAGGAAGCGA AAGACAGAGC TCAGGCAGCT CTGGAAAAAG CTAGCGATAC CAAGAACAAA 
GTGGATCATT CTAACAATGA TCTAAGGGAC CTTATCAAAC AAATACGCCA ATTCCTCATG 
CAGGAGGGTG CAGATCCGGA CAGCATTGAG GCGGTGGCCA ATCGTGTGCT GGAGCTCTCA 
ATCCCTGCCT CTCCCAAGCA GATCCGACAC CTAGCAGACG AGATTAAAGA CCGTGTCAAG 
AGTCTGTCTA ATGTGGATGC CATTCTGGAG CAAACACAAA ATGACGTCCG CAAAGCAGAG 
CAACTGTTGC TGGATGCCAA GAAAGCTAGG AATAAGGCAG AGGGGGTGAA GAACACAGCT 
GAGAGTGTGA AAAAGGCCCT GAATGATGCC AGCAGAGCCC AGGCTGCAGC AGAGAAAGCC 
ATCCAGAAAG CTAAGAATGA CATTGGCTTA ACTCAGAACC AGCTGGCACA GATCCAGTCA 
GAGACCTCCG CTAGTGAGAG AGATCTTAAT GATGCAGTGG ACAGACTGGG AGACCTGGAG 
CGCCAGATCG AGGCATTAAA GACCAAACGT GCCAACAACA GCCTGGATGC TGCACGGGCA 
GAGGAGACGG CCACCATGGC ACGGGACAAA GCCAACGAAG CCAAAGAGAT CCTGGATGGT 
GAGCTGTCCG ATAAGTACCG TACTGTTCAG GGTCTGATGG ACAATAAGGC GAAAACCATG 
CAGGACGCCA AGCACAAAGC AGAGCAATTG AGAGATGAAG CCAAGGGGCT GCTGAAAGAT 
GCACAGGATA AACTGCAGAG GCTGGCAGAG CTTGAAAAGG ACTATGAAGA AAACCAGAAG 
GTATTGGAAG GCAAAGCCAG GCAGCTGGAT GGCCTGGAAG ATAAAATGAA GGCGATCCTG 
AACGCCATCA ACAGGCAGAT TCAGATCTAC AACACCTGTC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LAMB2 ( XM_005162045.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005162102.1 Danio rerio laminin, beta 2 (laminin S) (lamb2), transcript variant X1, mRNA.

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CloneID	ODa27565
Clone ID Related Accession (Same CDS sequence)	XM_017353525.1 , XM_005162046.2 , XM_017353525.2 , XM_005162046.3 , XM_005162045.3 , XM_005162045.2
Accession Version	XM_005162045.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	laminin subunit beta-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005162045.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGCGC TTCTGTTGCT GTCCATCTGT GTCCTGTCAG CGGTCAGTGC ACAGGAGCCT 
GATGCTGCTC ACGGCTGCAC TCATGGAAGC TGCTATCCTG CAACCGGTGA CCTCTTGGTG 
GGTCGAGAAG CCAACCTGAA AGCATCATCC ACCTGTGGCC TGCGGAGGAG AGAACCCTAC 
TGCATTGTCA GCCATCTGCA GGAGGAGAAG AAGTGTTTCC AGTGTGATTC ACGGCGGCCT 
TATGATCCTC AGTCCAACCC CGTAAGCCAC CGCATCGAGA ACGTCATCAC CACCTTCAAA 
CCACATCGCA AGAAGTCCTG GTGGCAGTCG GAAAACGGGA AGTCTGATGT TTACCTTCAG 
CTGGATCTGG AGGCTGAGTT TCACTTCACT CATCTCATCA TGACTTTTAA GACTTTCCGT 
CCAGCAGCCA TGTTGATTGA GAGATCTGCA GATTTCGGCC GAAGCTGGCA GGTGTATCGC 
TACTTTGCTA ACGACTGCGA GTCCATCTTT CCAGGAATTT CCCAAGATTC TCTTCGAAAG 
GTTGATGATG TCATCTGCGA GTCCAGATAT TCTGATATCG AGCCGTCGAC AGAAGGAGAG 
GTGATTTTTC GTGTTCTTGA CCCTGCTATC AGGATCGAGG ACCCTTACAG CCCAAGAATT 
CAGAACCAGC TGAAGATCAC AAATCTTCGC CTGAATCTTA CCAAGCTGCA CACTCTGGGT 
GACAATCTGC TGGACTCACG GCCTGAGATC AAGGAGAAAT ACTACTATGC CATGTATGAG 
CTGGTCGTCA GGGGCAACTG CTTCTGCTAT GGCCACGCCT CTGAGTGTGC GCCAATTGAG 
GGCATCAGGG ATGACATTGA AGGCATGGTT CACGGGAGGT GTGTGTGCAA ACACAACACT 
AAAGGACTGA ACTGTGAACA GTGTGACGAC TTCTACAATG ATCTGCCCTG GAGACCAGCA 
GAAGGCCGGA ACACTAATGC CTGCAAGAAA TGCAACTGTA ATGGTCACTC CAACCAGTGT 
CATTTTGACA TGGCGGTGTA TTTGGCAACG GGGAACATCA GTGGAGGTGT GTGTGACAAC 
TGCCTTCACA ACACCATGGG CAGCAACTGT GAGAGCTGTA AACCTTTCTA CTACCAGGAT 
CCAACTAGAG ATATTAGAGA CCCTGGAGTC TGTGTAGCTT GCGATTGTGA CCCAGATGGC 
TCTAAGAACG GTGGTGTTTG TGACGGCCAT GATGACCCTT CTCTGGGCAT GATTGCTGGA 
CAGTGCCGCT GTAAAGACAA TGTTGAAGGT TCACGCTGTG ATAAATGCAA GCCTGGTTTC 
TTTGGCCTGA GTGCTAGTGA TCCACGTGGT TGTCAACCAT GTAAATGTGA CCCCAGGGGT 
ACAGTTTCCG GCAGCTCCCA ATGTGACCCT GTGAGCGGAG ACTGCTTCTG TAAACGCCTG 
GTCACTGGAC ACAGCTGCAA CCAATGTCTG CCTGAGCACT GGGCCTTGAG TCATGATACC 
AGTGGCTGCA GAGGGTGTGA CTGTGATGTG GGCGGAGCTA CAGAAAACCA ATGCTCAATG 
GAAACAGGGC AGTGTCAGTG TCGAAGCCAC ATGATTGGTC GCCAGTGCAA CCAGGTGGAG 
TCTGGGTACT TTTTCATGGC ACTTGACCAC TACATCTATG AAGCTGAAAC TGCCAAACTG 
GGTCAAGGCT CTGTTTTTGA GGAGAGAGAG TACCAGCCGG GTCGCCCCAT CTCATGGACA 
GGCACAGGCT TTGCACGAGC TCCTGAGGGC GGGACGCTGG AATTCGCAAT TAACAACATC 
CCATACTCTA TGGAATATGA CGTCCTGATT CGCTATGAGC TTCAGATGCC ACGTGACTGG 
GAAGAGGTGC GTGTTGTAGT GATTAGACCT GGTCCTATAC CTACCAGCAG TCCATGTGGA 
AACACCATCC CTGCAGATGA TCTGCTAACG GTGTCGCTTT CCTCTGGTGC CAGGTTTGTG 
GTTCTGCCTC AGCCAGTGTG TTTGGAGAGT GGTGTGAGTT ACAAACTGCG CGTGGAGCTC 
ATCCGTTACG CTGACAGGAA CAGCATCATT ACCTCCACCA ATGCCTTTGT GCTTGTTGAC 
TCTATCGCAC TTCTCCCCCG TTACTTGTCT CTGGAGATGT TTACTGCAGG AGACCCGGCC 
TCTGTCGCAC GCAAGCAGAG TTTTGAGCGT TACCGCTGCC ACGAAACTGC CAAGAGTGTG 
TCCCGTCCGC AGATGAGTGA AGTGTGTGCC AAACTCATCA CCAGCATGTC TGCCCTCATT 
AATGACGGCG CGCTGGCGTG TGAGTGTGAT CCTCAAGGTT CGCTCAGCTC TGAGTGTGAT 
GTCCGTGGCG GTCAGTGCCG CTGCAGGCCC AACATATCTG GCCGGCGCTG TGAGAAATGT 
GCACCAGGAG CTTACGGCTT TGGCCCATCT GGATGCAAAC TTTGTGAATG CAGTTTAGAG 
GGCTCTCAGA GCCGGTTCTG TGACCTGTAC TCAGGACAGT GTCCATGCCG ACCTGGAGCA 
TATGGTCAGC GCTGTGATGG TTGTCAGGCA GGACACTGGG GTTTCCCAAA CTGCAGGCCA 
TGTCAATGTA ACGGCCATGC TGACGAGTGC CATCAGAGGA CTGGAGCCTG TCTCAACTGC 
AGAAGTAATA CTGCAGGAGA CAAATGTGAA AGGTGTGCTA ATGGCTACTA TGGTAACCCA 
GTCCTTGGTT TAGGGAGTCA GTGTCGGCCC TGCCCCTGTC CTGAGGGCCC CAACAGCGGA 
CGCCACTTTG CTGCCTCTTG TTACCAAGAC AACCGAAGCC AACAGGTGGT CTGCAACTGC 
AACCAGGGTT ACACAGGGAC TGTAAAGCGC TCTGCAGGGG CAGTCCTCAA TCGTCCACGT 
TGTGATGAAT GTGCTCCTGG CTATTTTGGT GACCCGTCTA AGCCCGGTGG GCAGTGCCAG 
CCATGTCGTT GCAGTAATAA CATCGACCTG TCAGATCCTG AGTCTTGTGA CAAGCGCACT 
GGCCAGTGTC TGAAGTGCCT GTACAACACT GAAGGGCCAG ACTGTAGTGT GTGCAGGAGC 
GGATACTATG GAGACGCTTC TCGACGCAAC TGCCGGAAGT GCACCTGTAA CTTCCTGGGC 
ACAGAGCGCA GTCAGTGTCT GTCTCGAGAT GACTGTGTGT GTCAGCGTGC CACTGGTCAG 
TGTCAGTGTC TGCCTAATGT TATTGGACAA ACCTGTGATC ACTGCGCTCC AAACTCCTGG 
AACCTGGCCA GCGGGCAAGG CTGTGAGCCC TGCGCATGTG ACCCTAACAA TGCCTACACA 
TCTGCCTGTA ATGAGTTTAC AGGCCAGTGC CAGTGCCGTG TTGGGTTTGG AGGAAAGACC 
TGTACAGACT GTCAGGAGAA CCACTGGGGT GACCCAAGAG TGCTATGCCG AGCATGTGAC 
TGTGACCCCC GTGGCATCGA GTCATCCCAA TGCAACCGTA TGACGGGTCA CTGCGTGTGC 
CAGCAGGGTG TTTCTGGTGT CCGCTGTGAT CAGTGTGCCC GTGGCTTCTC CGGGACCTTC 
CCTGACTGTA AGCCTTGCCA CCAGTGTTTT GGGGACTGGG ACCGTATCGT TCAGGATTTG 
GCCACCAGAA CTAAAGCCCT CACAGATCGA GCCAAGGAGC TTCAGACCAC CGGCCTGACT 
CGAGCATTCG AGAGACGCTT TAAGGAGCTG GAAGACATGC TGGCACAGGC ACGGGACATT 
GTTAATGCCC GTAATGCCAC TGCTGAGGCT GTGACCACCC TGATGGGCAT GATTGAGGTT 
CTCAGAGCTC AGATTGGCGA GACCACAGAC ACACTGAACC AGCAGGAGGG AGACCTGACC 
GCCGTGCAGG ACAGTAACTA TGAAGCCAGT AATGCTTTAA GCACACTGGA GAGAGAAGCA 
AAAGAGCTCA ACCTGAACGC AGATCAGCTT AACCGTCAGC TTGACATCCT CAAGAACTCC 
AACTTCTTGG GTGCATATGA CAGCATCCGG GCCTCGTATA ATAAGTCACG TGACGCAGAG 
CACCGATCAA ACAGATCTAC CACTGACACT CCCAGCACAG TCAGCCAATC AGCAGACACC 
CGCAAGAAAA CAGAGCGGCT GATTGGCCAA AAGAGAGATG ATTTTAACCG CAAGAACGCT 
GCTAACAAGC GTACACTCAC TGACTTAAAT GCAAAAGTTC AGAATCTGGA CTTAAAGAAA 
ATCAACGAAA AGGTGTGTGG AGCTCCAGGT GATGCTCAGT GTGTTGACAG TCCTTGTGGT 
GGTGCTGGTT GTCGTGATGA TGAAGGCAAG CGTCGATGTG GAGGCCTGAA CTGTAATGGA 
GCTGTTGCAG TGGCGGACAC TGCTCTGGAT AGATCCAAAC ATGCAGAAAA AGAGCTGGAT 
AAAGCAATGG GGGTAGTGGA GGAGCTTTTT AAGCAGGTGG CGGATGCTAA GACCAAGGCA 
CAGGAAGCGA AAGACAGAGC TCAGGCAGCT CTGGAAAAAG CTAGCGATAC CAAGAACAAA 
GTGGATCATT CTAACAATGA TCTAAGGGAC CTTATCAAAC AAATACGCCA ATTCCTCATG 
CAGGAGGGTG CAGATCCGGA CAGCATTGAG GCGGTGGCCA ATCGTGTGCT GGAGCTCTCA 
ATCCCTGCCT CTCCCAAGCA GATCCGACAC CTAGCAGACG AGATTAAAGA CCGTGTCAAG 
AGTCTGTCTA ATGTGGATGC CATTCTGGAG CAAACACAAA ATGACGTCCG CAAAGCAGAG 
CAACTGTTGC TGGATGCCAA GAAAGCTAGG AATAAGGCAG AGGGGGTGAA GAACACAGCT 
GAGAGTGTGA AAAAGGCCCT GAATGATGCC AGCAGAGCCC AGGCTGCAGC AGAGAAAGCC 
ATCCAGAAAG CTAAGAATGA CATTGGCTTA ACTCAGAACC AGCTGGCACA GATCCAGTCA 
GAGACCTCCG CTAGTGAGAG AGATCTTAAT GATGCAGTGG ACAGACTGGG AGACCTGGAG 
CGCCAGATCG AGGCATTAAA GACCAAACGT GCCAACAACA GCCTGGATGC TGCACGGGCA 
GAGGAGACGG CCACCATGGC ACGGGACAAA GCCAACGAAG CCAAAGAGAT CCTGGATGGT 
GAGCTGTCCG ATAAGTACCG TACTGTTCAG GGTCTGATGG ACAATAAGGC GAAAACCATG 
CAGGACGCCA AGCACAAAGC AGAGCAATTG AGAGATGAAG CCAAGGGGCT GCTGAAAGAT 
GCACAGGATA AACTGCAGAG GCTGGCAGAG CTTGAAAAGG ACTATGAAGA AAACCAGAAG 
GTATTGGAAG GCAAAGCCAG GCAGCTGGAT GGCCTGGAAG ATAAAATGAA GGCGATCCTG 
AACGCCATCA ACAGGCAGAT TCAGATCTAC AACACCTGTC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162102.1
CDS	1150..6534
Translation	MRALLLLSICVLSAVSAQEPDAAHGCTHGSCYPATGDLLVGREANLKASSTCGLRRREPYCIVSHLQEEKKCFQCDSRRPYDPQSNPVSHRIENVITTFKPHRKKSWWQSENGKSDVYLQLDLEAEFHFTHLIMTFKTFRPAAMLIERSADFGRSWQVYRYFANDCESIFPGISQDSLRKVDDVICESRYSDIEPSTEGEVIFRVLDPAIRIEDPYSPRIQNQLKITNLRLNLTKLHTLGDNLLDSRPEIKEKYYYAMYELVVRGNCFCYGHASECAPIEGIRDDIEGMVHGRCVCKHNTKGLNCEQCDDFYNDLPWRPAEGRNTNACKKCNCNGHSNQCHFDMAVYLATGNISGGVCDNCLHNTMGSNCESCKPFYYQDPTRDIRDPGVCVACDCDPDGSKNGGVCDGHDDPSLGMIAGQCRCKDNVEGSRCDKCKPGFFGLSASDPRGCQPCKCDPRGTVSGSSQCDPVSGDCFCKRLVTGHSCNQCLPEHWALSHDTSGCRGCDCDVGGATENQCSMETGQCQCRSHMIGRQCNQVESGYFFMALDHYIYEAETAKLGQGSVFEEREYQPGRPISWTGTGFARAPEGGTLEFAINNIPYSMEYDVLIRYELQMPRDWEEVRVVVIRPGPIPTSSPCGNTIPADDLLTVSLSSGARFVVLPQPVCLESGVSYKLRVELIRYADRNSIITSTNAFVLVDSIALLPRYLSLEMFTAGDPASVARKQSFERYRCHETAKSVSRPQMSEVCAKLITSMSALINDGALACECDPQGSLSSECDVRGGQCRCRPNISGRRCEKCAPGAYGFGPSGCKLCECSLEGSQSRFCDLYSGQCPCRPGAYGQRCDGCQAGHWGFPNCRPCQCNGHADECHQRTGACLNCRSNTAGDKCERCANGYYGNPVLGLGSQCRPCPCPEGPNSGRHFAASCYQDNRSQQVVCNCNQGYTGTVKRSAGAVLNRPRCDECAPGYFGDPSKPGGQCQPCRCSNNIDLSDPESCDKRTGQCLKCLYNTEGPDCSVCRSGYYGDASRRNCRKCTCNFLGTERSQCLSRDDCVCQRATGQCQCLPNVIGQTCDHCAPNSWNLASGQGCEPCACDPNNAYTSACNEFTGQCQCRVGFGGKTCTDCQENHWGDPRVLCRACDCDPRGIESSQCNRMTGHCVCQQGVSGVRCDQCARGFSGTFPDCKPCHQCFGDWDRIVQDLATRTKALTDRAKELQTTGLTRAFERRFKELEDMLAQARDIVNARNATAEAVTTLMGMIEVLRAQIGETTDTLNQQEGDLTAVQDSNYEASNALSTLEREAKELNLNADQLNRQLDILKNSNFLGAYDSIRASYNKSRDAEHRSNRSTTDTPSTVSQSADTRKKTERLIGQKRDDFNRKNAANKRTLTDLNAKVQNLDLKKINEKVCGAPGDAQCVDSPCGGAGCRDDEGKRRCGGLNCNGAVAVADTALDRSKHAEKELDKAMGVVEELFKQVADAKTKAQEAKDRAQAALEKASDTKNKVDHSNNDLRDLIKQIRQFLMQEGADPDSIEAVANRVLELSIPASPKQIRHLADEIKDRVKSLSNVDAILEQTQNDVRKAEQLLLDAKKARNKAEGVKNTAESVKKALNDASRAQAAAEKAIQKAKNDIGLTQNQLAQIQSETSASERDLNDAVDRLGDLERQIEALKTKRANNSLDAARAEETATMARDKANEAKEILDGELSDKYRTVQGLMDNKAKTMQDAKHKAEQLRDEAKGLLKDAQDKLQRLAELEKDYEENQKVLEGKARQLDGLEDKMKAILNAINRQIQIYNTCQ

Target ORF information:

RefSeq Version	XM_005162045.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio laminin, beta 2 (laminin S) (lamb2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162045.3

ORF Insert Sequence:

ATGCGCGCGC TTCTGTTGCT GTCCATCTGT GTCCTGTCAG CGGTCAGTGC ACAGGAGCCT 
GATGCTGCTC ACGGCTGCAC TCATGGAAGC TGCTATCCTG CAACCGGTGA CCTCTTGGTG 
GGTCGAGAAG CCAACCTGAA AGCATCATCC ACCTGTGGCC TGCGGAGGAG AGAACCCTAC 
TGCATTGTCA GCCATCTGCA GGAGGAGAAG AAGTGTTTCC AGTGTGATTC ACGGCGGCCT 
TATGATCCTC AGTCCAACCC CGTAAGCCAC CGCATCGAGA ACGTCATCAC CACCTTCAAA 
CCACATCGCA AGAAGTCCTG GTGGCAGTCG GAAAACGGGA AGTCTGATGT TTACCTTCAG 
CTGGATCTGG AGGCTGAGTT TCACTTCACT CATCTCATCA TGACTTTTAA GACTTTCCGT 
CCAGCAGCCA TGTTGATTGA GAGATCTGCA GATTTCGGCC GAAGCTGGCA GGTGTATCGC 
TACTTTGCTA ACGACTGCGA GTCCATCTTT CCAGGAATTT CCCAAGATTC TCTTCGAAAG 
GTTGATGATG TCATCTGCGA GTCCAGATAT TCTGATATCG AGCCGTCGAC AGAAGGAGAG 
GTGATTTTTC GTGTTCTTGA CCCTGCTATC AGGATCGAGG ACCCTTACAG CCCAAGAATT 
CAGAACCAGC TGAAGATCAC AAATCTTCGC CTGAATCTTA CCAAGCTGCA CACTCTGGGT 
GACAATCTGC TGGACTCACG GCCTGAGATC AAGGAGAAAT ACTACTATGC CATGTATGAG 
CTGGTCGTCA GGGGCAACTG CTTCTGCTAT GGCCACGCCT CTGAGTGTGC GCCAATTGAG 
GGCATCAGGG ATGACATTGA AGGCATGGTT CACGGGAGGT GTGTGTGCAA ACACAACACT 
AAAGGACTGA ACTGTGAACA GTGTGACGAC TTCTACAATG ATCTGCCCTG GAGACCAGCA 
GAAGGCCGGA ACACTAATGC CTGCAAGAAA TGCAACTGTA ATGGTCACTC CAACCAGTGT 
CATTTTGACA TGGCGGTGTA TTTGGCAACG GGGAACATCA GTGGAGGTGT GTGTGACAAC 
TGCCTTCACA ACACCATGGG CAGCAACTGT GAGAGCTGTA AACCTTTCTA CTACCAGGAT 
CCAACTAGAG ATATTAGAGA CCCTGGAGTC TGTGTAGCTT GCGATTGTGA CCCAGATGGC 
TCTAAGAACG GTGGTGTTTG TGACGGCCAT GATGACCCTT CTCTGGGCAT GATTGCTGGA 
CAGTGCCGCT GTAAAGACAA TGTTGAAGGT TCACGCTGTG ATAAATGCAA GCCTGGTTTC 
TTTGGCCTGA GTGCTAGTGA TCCACGTGGT TGTCAACCAT GTAAATGTGA CCCCAGGGGT 
ACAGTTTCCG GCAGCTCCCA ATGTGACCCT GTGAGCGGAG ACTGCTTCTG TAAACGCCTG 
GTCACTGGAC ACAGCTGCAA CCAATGTCTG CCTGAGCACT GGGCCTTGAG TCATGATACC 
AGTGGCTGCA GAGGGTGTGA CTGTGATGTG GGCGGAGCTA CAGAAAACCA ATGCTCAATG 
GAAACAGGGC AGTGTCAGTG TCGAAGCCAC ATGATTGGTC GCCAGTGCAA CCAGGTGGAG 
TCTGGGTACT TTTTCATGGC ACTTGACCAC TACATCTATG AAGCTGAAAC TGCCAAACTG 
GGTCAAGGCT CTGTTTTTGA GGAGAGAGAG TACCAGCCGG GTCGCCCCAT CTCATGGACA 
GGCACAGGCT TTGCACGAGC TCCTGAGGGC GGGACGCTGG AATTCGCAAT TAACAACATC 
CCATACTCTA TGGAATATGA CGTCCTGATT CGCTATGAGC TTCAGATGCC ACGTGACTGG 
GAAGAGGTGC GTGTTGTAGT GATTAGACCT GGTCCTATAC CTACCAGCAG TCCATGTGGA 
AACACCATCC CTGCAGATGA TCTGCTAACG GTGTCGCTTT CCTCTGGTGC CAGGTTTGTG 
GTTCTGCCTC AGCCAGTGTG TTTGGAGAGT GGTGTGAGTT ACAAACTGCG CGTGGAGCTC 
ATCCGTTACG CTGACAGGAA CAGCATCATT ACCTCCACCA ATGCCTTTGT GCTTGTTGAC 
TCTATCGCAC TTCTCCCCCG TTACTTGTCT CTGGAGATGT TTACTGCAGG AGACCCGGCC 
TCTGTCGCAC GCAAGCAGAG TTTTGAGCGT TACCGCTGCC ACGAAACTGC CAAGAGTGTG 
TCCCGTCCGC AGATGAGTGA AGTGTGTGCC AAACTCATCA CCAGCATGTC TGCCCTCATT 
AATGACGGCG CGCTGGCGTG TGAGTGTGAT CCTCAAGGTT CGCTCAGCTC TGAGTGTGAT 
GTCCGTGGCG GTCAGTGCCG CTGCAGGCCC AACATATCTG GCCGGCGCTG TGAGAAATGT 
GCACCAGGAG CTTACGGCTT TGGCCCATCT GGATGCAAAC TTTGTGAATG CAGTTTAGAG 
GGCTCTCAGA GCCGGTTCTG TGACCTGTAC TCAGGACAGT GTCCATGCCG ACCTGGAGCA 
TATGGTCAGC GCTGTGATGG TTGTCAGGCA GGACACTGGG GTTTCCCAAA CTGCAGGCCA 
TGTCAATGTA ACGGCCATGC TGACGAGTGC CATCAGAGGA CTGGAGCCTG TCTCAACTGC 
AGAAGTAATA CTGCAGGAGA CAAATGTGAA AGGTGTGCTA ATGGCTACTA TGGTAACCCA 
GTCCTTGGTT TAGGGAGTCA GTGTCGGCCC TGCCCCTGTC CTGAGGGCCC CAACAGCGGA 
CGCCACTTTG CTGCCTCTTG TTACCAAGAC AACCGAAGCC AACAGGTGGT CTGCAACTGC 
AACCAGGGTT ACACAGGGAC TGTAAAGCGC TCTGCAGGGG CAGTCCTCAA TCGTCCACGT 
TGTGATGAAT GTGCTCCTGG CTATTTTGGT GACCCGTCTA AGCCCGGTGG GCAGTGCCAG 
CCATGTCGTT GCAGTAATAA CATCGACCTG TCAGATCCTG AGTCTTGTGA CAAGCGCACT 
GGCCAGTGTC TGAAGTGCCT GTACAACACT GAAGGGCCAG ACTGTAGTGT GTGCAGGAGC 
GGATACTATG GAGACGCTTC TCGACGCAAC TGCCGGAAGT GCACCTGTAA CTTCCTGGGC 
ACAGAGCGCA GTCAGTGTCT GTCTCGAGAT GACTGTGTGT GTCAGCGTGC CACTGGTCAG 
TGTCAGTGTC TGCCTAATGT TATTGGACAA ACCTGTGATC ACTGCGCTCC AAACTCCTGG 
AACCTGGCCA GCGGGCAAGG CTGTGAGCCC TGCGCATGTG ACCCTAACAA TGCCTACACA 
TCTGCCTGTA ATGAGTTTAC AGGCCAGTGC CAGTGCCGTG TTGGGTTTGG AGGAAAGACC 
TGTACAGACT GTCAGGAGAA CCACTGGGGT GACCCAAGAG TGCTATGCCG AGCATGTGAC 
TGTGACCCCC GTGGCATCGA GTCATCCCAA TGCAACCGTA TGACGGGTCA CTGCGTGTGC 
CAGCAGGGTG TTTCTGGTGT CCGCTGTGAT CAGTGTGCCC GTGGCTTCTC CGGGACCTTC 
CCTGACTGTA AGCCTTGCCA CCAGTGTTTT GGGGACTGGG ACCGTATCGT TCAGGATTTG 
GCCACCAGAA CTAAAGCCCT CACAGATCGA GCCAAGGAGC TTCAGACCAC CGGCCTGACT 
CGAGCATTCG AGAGACGCTT TAAGGAGCTG GAAGACATGC TGGCACAGGC ACGGGACATT 
GTTAATGCCC GTAATGCCAC TGCTGAGGCT GTGACCACCC TGATGGGCAT GATTGAGGTT 
CTCAGAGCTC AGATTGGCGA GACCACAGAC ACACTGAACC AGCAGGAGGG AGACCTGACC 
GCCGTGCAGG ACAGTAACTA TGAAGCCAGT AATGCTTTAA GCACACTGGA GAGAGAAGCA 
AAAGAGCTCA ACCTGAACGC AGATCAGCTT AACCGTCAGC TTGACATCCT CAAGAACTCC 
AACTTCTTGG GTGCATATGA CAGCATCCGG GCCTCGTATA ATAAGTCACG TGACGCAGAG 
CACCGATCAA ACAGATCTAC CACTGACACT CCCAGCACAG TCAGCCAATC AGCAGACACC 
CGCAAGAAAA CAGAGCGGCT GATTGGCCAA AAGAGAGATG ATTTTAACCG CAAGAACGCT 
GCTAACAAGC GTACACTCAC TGACTTAAAT GCAAAAGTTC AGAATCTGGA CTTAAAGAAA 
ATCAACGAAA AGGTGTGTGG AGCTCCAGGT GATGCTCAGT GTGTTGACAG TCCTTGTGGT 
GGTGCTGGTT GTCGTGATGA TGAAGGCAAG CGTCGATGTG GAGGCCTGAA CTGTAATGGA 
GCTGTTGCAG TGGCGGACAC TGCTCTGGAT AGATCCAAAC ATGCAGAAAA AGAGCTGGAT 
AAAGCAATGG GGGTAGTGGA GGAGCTTTTT AAGCAGGTGG CGGATGCTAA GACCAAGGCA 
CAGGAAGCGA AAGACAGAGC TCAGGCAGCT CTGGAAAAAG CTAGCGATAC CAAGAACAAA 
GTGGATCATT CTAACAATGA TCTAAGGGAC CTTATCAAAC AAATACGCCA ATTCCTCATG 
CAGGAGGGTG CAGATCCGGA CAGCATTGAG GCGGTGGCCA ATCGTGTGCT GGAGCTCTCA 
ATCCCTGCCT CTCCCAAGCA GATCCGACAC CTAGCAGACG AGATTAAAGA CCGTGTCAAG 
AGTCTGTCTA ATGTGGATGC CATTCTGGAG CAAACACAAA ATGACGTCCG CAAAGCAGAG 
CAACTGTTGC TGGATGCCAA GAAAGCTAGG AATAAGGCAG AGGGGGTGAA GAACACAGCT 
GAGAGTGTGA AAAAGGCCCT GAATGATGCC AGCAGAGCCC AGGCTGCAGC AGAGAAAGCC 
ATCCAGAAAG CTAAGAATGA CATTGGCTTA ACTCAGAACC AGCTGGCACA GATCCAGTCA 
GAGACCTCCG CTAGTGAGAG AGATCTTAAT GATGCAGTGG ACAGACTGGG AGACCTGGAG 
CGCCAGATCG AGGCATTAAA GACCAAACGT GCCAACAACA GCCTGGATGC TGCACGGGCA 
GAGGAGACGG CCACCATGGC ACGGGACAAA GCCAACGAAG CCAAAGAGAT CCTGGATGGT 
GAGCTGTCCG ATAAGTACCG TACTGTTCAG GGTCTGATGG ACAATAAGGC GAAAACCATG 
CAGGACGCCA AGCACAAAGC AGAGCAATTG AGAGATGAAG CCAAGGGGCT GCTGAAAGAT 
GCACAGGATA AACTGCAGAG GCTGGCAGAG CTTGAAAAGG ACTATGAAGA AAACCAGAAG 
GTATTGGAAG GCAAAGCCAG GCAGCTGGAT GGCCTGGAAG ATAAAATGAA GGCGATCCTG 
AACGCCATCA ACAGGCAGAT TCAGATCTAC AACACCTGTC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LAMB2 ( XM_005162045.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005162102.1 Danio rerio laminin, beta 2 (laminin S) (lamb2), transcript variant X1, mRNA.

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CloneID	ODa27565
Clone ID Related Accession (Same CDS sequence)	XM_017353525.1 , XM_005162046.2 , XM_017353525.2 , XM_005162046.3 , XM_005162045.3 , XM_005162045.2
Accession Version	XM_005162045.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	laminin subunit beta-2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2014 this sequence version replaced XM_005162045.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGCGC TTCTGTTGCT GTCCATCTGT GTCCTGTCAG CGGTCAGTGC ACAGGAGCCT 
GATGCTGCTC ACGGCTGCAC TCATGGAAGC TGCTATCCTG CAACCGGTGA CCTCTTGGTG 
GGTCGAGAAG CCAACCTGAA AGCATCATCC ACCTGTGGCC TGCGGAGGAG AGAACCCTAC 
TGCATTGTCA GCCATCTGCA GGAGGAGAAG AAGTGTTTCC AGTGTGATTC ACGGCGGCCT 
TATGATCCTC AGTCCAACCC CGTAAGCCAC CGCATCGAGA ACGTCATCAC CACCTTCAAA 
CCACATCGCA AGAAGTCCTG GTGGCAGTCG GAAAACGGGA AGTCTGATGT TTACCTTCAG 
CTGGATCTGG AGGCTGAGTT TCACTTCACT CATCTCATCA TGACTTTTAA GACTTTCCGT 
CCAGCAGCCA TGTTGATTGA GAGATCTGCA GATTTCGGCC GAAGCTGGCA GGTGTATCGC 
TACTTTGCTA ACGACTGCGA GTCCATCTTT CCAGGAATTT CCCAAGATTC TCTTCGAAAG 
GTTGATGATG TCATCTGCGA GTCCAGATAT TCTGATATCG AGCCGTCGAC AGAAGGAGAG 
GTGATTTTTC GTGTTCTTGA CCCTGCTATC AGGATCGAGG ACCCTTACAG CCCAAGAATT 
CAGAACCAGC TGAAGATCAC AAATCTTCGC CTGAATCTTA CCAAGCTGCA CACTCTGGGT 
GACAATCTGC TGGACTCACG GCCTGAGATC AAGGAGAAAT ACTACTATGC CATGTATGAG 
CTGGTCGTCA GGGGCAACTG CTTCTGCTAT GGCCACGCCT CTGAGTGTGC GCCAATTGAG 
GGCATCAGGG ATGACATTGA AGGCATGGTT CACGGGAGGT GTGTGTGCAA ACACAACACT 
AAAGGACTGA ACTGTGAACA GTGTGACGAC TTCTACAATG ATCTGCCCTG GAGACCAGCA 
GAAGGCCGGA ACACTAATGC CTGCAAGAAA TGCAACTGTA ATGGTCACTC CAACCAGTGT 
CATTTTGACA TGGCGGTGTA TTTGGCAACG GGGAACATCA GTGGAGGTGT GTGTGACAAC 
TGCCTTCACA ACACCATGGG CAGCAACTGT GAGAGCTGTA AACCTTTCTA CTACCAGGAT 
CCAACTAGAG ATATTAGAGA CCCTGGAGTC TGTGTAGCTT GCGATTGTGA CCCAGATGGC 
TCTAAGAACG GTGGTGTTTG TGACGGCCAT GATGACCCTT CTCTGGGCAT GATTGCTGGA 
CAGTGCCGCT GTAAAGACAA TGTTGAAGGT TCACGCTGTG ATAAATGCAA GCCTGGTTTC 
TTTGGCCTGA GTGCTAGTGA TCCACGTGGT TGTCAACCAT GTAAATGTGA CCCCAGGGGT 
ACAGTTTCCG GCAGCTCCCA ATGTGACCCT GTGAGCGGAG ACTGCTTCTG TAAACGCCTG 
GTCACTGGAC ACAGCTGCAA CCAATGTCTG CCTGAGCACT GGGCCTTGAG TCATGATACC 
AGTGGCTGCA GAGGGTGTGA CTGTGATGTG GGCGGAGCTA CAGAAAACCA ATGCTCAATG 
GAAACAGGGC AGTGTCAGTG TCGAAGCCAC ATGATTGGTC GCCAGTGCAA CCAGGTGGAG 
TCTGGGTACT TTTTCATGGC ACTTGACCAC TACATCTATG AAGCTGAAAC TGCCAAACTG 
GGTCAAGGCT CTGTTTTTGA GGAGAGAGAG TACCAGCCGG GTCGCCCCAT CTCATGGACA 
GGCACAGGCT TTGCACGAGC TCCTGAGGGC GGGACGCTGG AATTCGCAAT TAACAACATC 
CCATACTCTA TGGAATATGA CGTCCTGATT CGCTATGAGC TTCAGATGCC ACGTGACTGG 
GAAGAGGTGC GTGTTGTAGT GATTAGACCT GGTCCTATAC CTACCAGCAG TCCATGTGGA 
AACACCATCC CTGCAGATGA TCTGCTAACG GTGTCGCTTT CCTCTGGTGC CAGGTTTGTG 
GTTCTGCCTC AGCCAGTGTG TTTGGAGAGT GGTGTGAGTT ACAAACTGCG CGTGGAGCTC 
ATCCGTTACG CTGACAGGAA CAGCATCATT ACCTCCACCA ATGCCTTTGT GCTTGTTGAC 
TCTATCGCAC TTCTCCCCCG TTACTTGTCT CTGGAGATGT TTACTGCAGG AGACCCGGCC 
TCTGTCGCAC GCAAGCAGAG TTTTGAGCGT TACCGCTGCC ACGAAACTGC CAAGAGTGTG 
TCCCGTCCGC AGATGAGTGA AGTGTGTGCC AAACTCATCA CCAGCATGTC TGCCCTCATT 
AATGACGGCG CGCTGGCGTG TGAGTGTGAT CCTCAAGGTT CGCTCAGCTC TGAGTGTGAT 
GTCCGTGGCG GTCAGTGCCG CTGCAGGCCC AACATATCTG GCCGGCGCTG TGAGAAATGT 
GCACCAGGAG CTTACGGCTT TGGCCCATCT GGATGCAAAC TTTGTGAATG CAGTTTAGAG 
GGCTCTCAGA GCCGGTTCTG TGACCTGTAC TCAGGACAGT GTCCATGCCG ACCTGGAGCA 
TATGGTCAGC GCTGTGATGG TTGTCAGGCA GGACACTGGG GTTTCCCAAA CTGCAGGCCA 
TGTCAATGTA ACGGCCATGC TGACGAGTGC CATCAGAGGA CTGGAGCCTG TCTCAACTGC 
AGAAGTAATA CTGCAGGAGA CAAATGTGAA AGGTGTGCTA ATGGCTACTA TGGTAACCCA 
GTCCTTGGTT TAGGGAGTCA GTGTCGGCCC TGCCCCTGTC CTGAGGGCCC CAACAGCGGA 
CGCCACTTTG CTGCCTCTTG TTACCAAGAC AACCGAAGCC AACAGGTGGT CTGCAACTGC 
AACCAGGGTT ACACAGGGAC TGTAAAGCGC TCTGCAGGGG CAGTCCTCAA TCGTCCACGT 
TGTGATGAAT GTGCTCCTGG CTATTTTGGT GACCCGTCTA AGCCCGGTGG GCAGTGCCAG 
CCATGTCGTT GCAGTAATAA CATCGACCTG TCAGATCCTG AGTCTTGTGA CAAGCGCACT 
GGCCAGTGTC TGAAGTGCCT GTACAACACT GAAGGGCCAG ACTGTAGTGT GTGCAGGAGC 
GGATACTATG GAGACGCTTC TCGACGCAAC TGCCGGAAGT GCACCTGTAA CTTCCTGGGC 
ACAGAGCGCA GTCAGTGTCT GTCTCGAGAT GACTGTGTGT GTCAGCGTGC CACTGGTCAG 
TGTCAGTGTC TGCCTAATGT TATTGGACAA ACCTGTGATC ACTGCGCTCC AAACTCCTGG 
AACCTGGCCA GCGGGCAAGG CTGTGAGCCC TGCGCATGTG ACCCTAACAA TGCCTACACA 
TCTGCCTGTA ATGAGTTTAC AGGCCAGTGC CAGTGCCGTG TTGGGTTTGG AGGAAAGACC 
TGTACAGACT GTCAGGAGAA CCACTGGGGT GACCCAAGAG TGCTATGCCG AGCATGTGAC 
TGTGACCCCC GTGGCATCGA GTCATCCCAA TGCAACCGTA TGACGGGTCA CTGCGTGTGC 
CAGCAGGGTG TTTCTGGTGT CCGCTGTGAT CAGTGTGCCC GTGGCTTCTC CGGGACCTTC 
CCTGACTGTA AGCCTTGCCA CCAGTGTTTT GGGGACTGGG ACCGTATCGT TCAGGATTTG 
GCCACCAGAA CTAAAGCCCT CACAGATCGA GCCAAGGAGC TTCAGACCAC CGGCCTGACT 
CGAGCATTCG AGAGACGCTT TAAGGAGCTG GAAGACATGC TGGCACAGGC ACGGGACATT 
GTTAATGCCC GTAATGCCAC TGCTGAGGCT GTGACCACCC TGATGGGCAT GATTGAGGTT 
CTCAGAGCTC AGATTGGCGA GACCACAGAC ACACTGAACC AGCAGGAGGG AGACCTGACC 
GCCGTGCAGG ACAGTAACTA TGAAGCCAGT AATGCTTTAA GCACACTGGA GAGAGAAGCA 
AAAGAGCTCA ACCTGAACGC AGATCAGCTT AACCGTCAGC TTGACATCCT CAAGAACTCC 
AACTTCTTGG GTGCATATGA CAGCATCCGG GCCTCGTATA ATAAGTCACG TGACGCAGAG 
CACCGATCAA ACAGATCTAC CACTGACACT CCCAGCACAG TCAGCCAATC AGCAGACACC 
CGCAAGAAAA CAGAGCGGCT GATTGGCCAA AAGAGAGATG ATTTTAACCG CAAGAACGCT 
GCTAACAAGC GTACACTCAC TGACTTAAAT GCAAAAGTTC AGAATCTGGA CTTAAAGAAA 
ATCAACGAAA AGGTGTGTGG AGCTCCAGGT GATGCTCAGT GTGTTGACAG TCCTTGTGGT 
GGTGCTGGTT GTCGTGATGA TGAAGGCAAG CGTCGATGTG GAGGCCTGAA CTGTAATGGA 
GCTGTTGCAG TGGCGGACAC TGCTCTGGAT AGATCCAAAC ATGCAGAAAA AGAGCTGGAT 
AAAGCAATGG GGGTAGTGGA GGAGCTTTTT AAGCAGGTGG CGGATGCTAA GACCAAGGCA 
CAGGAAGCGA AAGACAGAGC TCAGGCAGCT CTGGAAAAAG CTAGCGATAC CAAGAACAAA 
GTGGATCATT CTAACAATGA TCTAAGGGAC CTTATCAAAC AAATACGCCA ATTCCTCATG 
CAGGAGGGTG CAGATCCGGA CAGCATTGAG GCGGTGGCCA ATCGTGTGCT GGAGCTCTCA 
ATCCCTGCCT CTCCCAAGCA GATCCGACAC CTAGCAGACG AGATTAAAGA CCGTGTCAAG 
AGTCTGTCTA ATGTGGATGC CATTCTGGAG CAAACACAAA ATGACGTCCG CAAAGCAGAG 
CAACTGTTGC TGGATGCCAA GAAAGCTAGG AATAAGGCAG AGGGGGTGAA GAACACAGCT 
GAGAGTGTGA AAAAGGCCCT GAATGATGCC AGCAGAGCCC AGGCTGCAGC AGAGAAAGCC 
ATCCAGAAAG CTAAGAATGA CATTGGCTTA ACTCAGAACC AGCTGGCACA GATCCAGTCA 
GAGACCTCCG CTAGTGAGAG AGATCTTAAT GATGCAGTGG ACAGACTGGG AGACCTGGAG 
CGCCAGATCG AGGCATTAAA GACCAAACGT GCCAACAACA GCCTGGATGC TGCACGGGCA 
GAGGAGACGG CCACCATGGC ACGGGACAAA GCCAACGAAG CCAAAGAGAT CCTGGATGGT 
GAGCTGTCCG ATAAGTACCG TACTGTTCAG GGTCTGATGG ACAATAAGGC GAAAACCATG 
CAGGACGCCA AGCACAAAGC AGAGCAATTG AGAGATGAAG CCAAGGGGCT GCTGAAAGAT 
GCACAGGATA AACTGCAGAG GCTGGCAGAG CTTGAAAAGG ACTATGAAGA AAACCAGAAG 
GTATTGGAAG GCAAAGCCAG GCAGCTGGAT GGCCTGGAAG ATAAAATGAA GGCGATCCTG 
AACGCCATCA ACAGGCAGAT TCAGATCTAC AACACCTGTC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162102.1
CDS	1161..6545
Translation	MRALLLLSICVLSAVSAQEPDAAHGCTHGSCYPATGDLLVGREANLKASSTCGLRRREPYCIVSHLQEEKKCFQCDSRRPYDPQSNPVSHRIENVITTFKPHRKKSWWQSENGKSDVYLQLDLEAEFHFTHLIMTFKTFRPAAMLIERSADFGRSWQVYRYFANDCESIFPGISQDSLRKVDDVICESRYSDIEPSTEGEVIFRVLDPAIRIEDPYSPRIQNQLKITNLRLNLTKLHTLGDNLLDSRPEIKEKYYYAMYELVVRGNCFCYGHASECAPIEGIRDDIEGMVHGRCVCKHNTKGLNCEQCDDFYNDLPWRPAEGRNTNACKKCNCNGHSNQCHFDMAVYLATGNISGGVCDNCLHNTMGSNCESCKPFYYQDPTRDIRDPGVCVACDCDPDGSKNGGVCDGHDDPSLGMIAGQCRCKDNVEGSRCDKCKPGFFGLSASDPRGCQPCKCDPRGTVSGSSQCDPVSGDCFCKRLVTGHSCNQCLPEHWALSHDTSGCRGCDCDVGGATENQCSMETGQCQCRSHMIGRQCNQVESGYFFMALDHYIYEAETAKLGQGSVFEEREYQPGRPISWTGTGFARAPEGGTLEFAINNIPYSMEYDVLIRYELQMPRDWEEVRVVVIRPGPIPTSSPCGNTIPADDLLTVSLSSGARFVVLPQPVCLESGVSYKLRVELIRYADRNSIITSTNAFVLVDSIALLPRYLSLEMFTAGDPASVARKQSFERYRCHETAKSVSRPQMSEVCAKLITSMSALINDGALACECDPQGSLSSECDVRGGQCRCRPNISGRRCEKCAPGAYGFGPSGCKLCECSLEGSQSRFCDLYSGQCPCRPGAYGQRCDGCQAGHWGFPNCRPCQCNGHADECHQRTGACLNCRSNTAGDKCERCANGYYGNPVLGLGSQCRPCPCPEGPNSGRHFAASCYQDNRSQQVVCNCNQGYTGTVKRSAGAVLNRPRCDECAPGYFGDPSKPGGQCQPCRCSNNIDLSDPESCDKRTGQCLKCLYNTEGPDCSVCRSGYYGDASRRNCRKCTCNFLGTERSQCLSRDDCVCQRATGQCQCLPNVIGQTCDHCAPNSWNLASGQGCEPCACDPNNAYTSACNEFTGQCQCRVGFGGKTCTDCQENHWGDPRVLCRACDCDPRGIESSQCNRMTGHCVCQQGVSGVRCDQCARGFSGTFPDCKPCHQCFGDWDRIVQDLATRTKALTDRAKELQTTGLTRAFERRFKELEDMLAQARDIVNARNATAEAVTTLMGMIEVLRAQIGETTDTLNQQEGDLTAVQDSNYEASNALSTLEREAKELNLNADQLNRQLDILKNSNFLGAYDSIRASYNKSRDAEHRSNRSTTDTPSTVSQSADTRKKTERLIGQKRDDFNRKNAANKRTLTDLNAKVQNLDLKKINEKVCGAPGDAQCVDSPCGGAGCRDDEGKRRCGGLNCNGAVAVADTALDRSKHAEKELDKAMGVVEELFKQVADAKTKAQEAKDRAQAALEKASDTKNKVDHSNNDLRDLIKQIRQFLMQEGADPDSIEAVANRVLELSIPASPKQIRHLADEIKDRVKSLSNVDAILEQTQNDVRKAEQLLLDAKKARNKAEGVKNTAESVKKALNDASRAQAAAEKAIQKAKNDIGLTQNQLAQIQSETSASERDLNDAVDRLGDLERQIEALKTKRANNSLDAARAEETATMARDKANEAKEILDGELSDKYRTVQGLMDNKAKTMQDAKHKAEQLRDEAKGLLKDAQDKLQRLAELEKDYEENQKVLEGKARQLDGLEDKMKAILNAINRQIQIYNTCQ

Target ORF information:

RefSeq Version	XM_005162045.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio laminin, beta 2 (laminin S) (lamb2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162045.2

ORF Insert Sequence:

ATGCGCGCGC TTCTGTTGCT GTCCATCTGT GTCCTGTCAG CGGTCAGTGC ACAGGAGCCT 
GATGCTGCTC ACGGCTGCAC TCATGGAAGC TGCTATCCTG CAACCGGTGA CCTCTTGGTG 
GGTCGAGAAG CCAACCTGAA AGCATCATCC ACCTGTGGCC TGCGGAGGAG AGAACCCTAC 
TGCATTGTCA GCCATCTGCA GGAGGAGAAG AAGTGTTTCC AGTGTGATTC ACGGCGGCCT 
TATGATCCTC AGTCCAACCC CGTAAGCCAC CGCATCGAGA ACGTCATCAC CACCTTCAAA 
CCACATCGCA AGAAGTCCTG GTGGCAGTCG GAAAACGGGA AGTCTGATGT TTACCTTCAG 
CTGGATCTGG AGGCTGAGTT TCACTTCACT CATCTCATCA TGACTTTTAA GACTTTCCGT 
CCAGCAGCCA TGTTGATTGA GAGATCTGCA GATTTCGGCC GAAGCTGGCA GGTGTATCGC 
TACTTTGCTA ACGACTGCGA GTCCATCTTT CCAGGAATTT CCCAAGATTC TCTTCGAAAG 
GTTGATGATG TCATCTGCGA GTCCAGATAT TCTGATATCG AGCCGTCGAC AGAAGGAGAG 
GTGATTTTTC GTGTTCTTGA CCCTGCTATC AGGATCGAGG ACCCTTACAG CCCAAGAATT 
CAGAACCAGC TGAAGATCAC AAATCTTCGC CTGAATCTTA CCAAGCTGCA CACTCTGGGT 
GACAATCTGC TGGACTCACG GCCTGAGATC AAGGAGAAAT ACTACTATGC CATGTATGAG 
CTGGTCGTCA GGGGCAACTG CTTCTGCTAT GGCCACGCCT CTGAGTGTGC GCCAATTGAG 
GGCATCAGGG ATGACATTGA AGGCATGGTT CACGGGAGGT GTGTGTGCAA ACACAACACT 
AAAGGACTGA ACTGTGAACA GTGTGACGAC TTCTACAATG ATCTGCCCTG GAGACCAGCA 
GAAGGCCGGA ACACTAATGC CTGCAAGAAA TGCAACTGTA ATGGTCACTC CAACCAGTGT 
CATTTTGACA TGGCGGTGTA TTTGGCAACG GGGAACATCA GTGGAGGTGT GTGTGACAAC 
TGCCTTCACA ACACCATGGG CAGCAACTGT GAGAGCTGTA AACCTTTCTA CTACCAGGAT 
CCAACTAGAG ATATTAGAGA CCCTGGAGTC TGTGTAGCTT GCGATTGTGA CCCAGATGGC 
TCTAAGAACG GTGGTGTTTG TGACGGCCAT GATGACCCTT CTCTGGGCAT GATTGCTGGA 
CAGTGCCGCT GTAAAGACAA TGTTGAAGGT TCACGCTGTG ATAAATGCAA GCCTGGTTTC 
TTTGGCCTGA GTGCTAGTGA TCCACGTGGT TGTCAACCAT GTAAATGTGA CCCCAGGGGT 
ACAGTTTCCG GCAGCTCCCA ATGTGACCCT GTGAGCGGAG ACTGCTTCTG TAAACGCCTG 
GTCACTGGAC ACAGCTGCAA CCAATGTCTG CCTGAGCACT GGGCCTTGAG TCATGATACC 
AGTGGCTGCA GAGGGTGTGA CTGTGATGTG GGCGGAGCTA CAGAAAACCA ATGCTCAATG 
GAAACAGGGC AGTGTCAGTG TCGAAGCCAC ATGATTGGTC GCCAGTGCAA CCAGGTGGAG 
TCTGGGTACT TTTTCATGGC ACTTGACCAC TACATCTATG AAGCTGAAAC TGCCAAACTG 
GGTCAAGGCT CTGTTTTTGA GGAGAGAGAG TACCAGCCGG GTCGCCCCAT CTCATGGACA 
GGCACAGGCT TTGCACGAGC TCCTGAGGGC GGGACGCTGG AATTCGCAAT TAACAACATC 
CCATACTCTA TGGAATATGA CGTCCTGATT CGCTATGAGC TTCAGATGCC ACGTGACTGG 
GAAGAGGTGC GTGTTGTAGT GATTAGACCT GGTCCTATAC CTACCAGCAG TCCATGTGGA 
AACACCATCC CTGCAGATGA TCTGCTAACG GTGTCGCTTT CCTCTGGTGC CAGGTTTGTG 
GTTCTGCCTC AGCCAGTGTG TTTGGAGAGT GGTGTGAGTT ACAAACTGCG CGTGGAGCTC 
ATCCGTTACG CTGACAGGAA CAGCATCATT ACCTCCACCA ATGCCTTTGT GCTTGTTGAC 
TCTATCGCAC TTCTCCCCCG TTACTTGTCT CTGGAGATGT TTACTGCAGG AGACCCGGCC 
TCTGTCGCAC GCAAGCAGAG TTTTGAGCGT TACCGCTGCC ACGAAACTGC CAAGAGTGTG 
TCCCGTCCGC AGATGAGTGA AGTGTGTGCC AAACTCATCA CCAGCATGTC TGCCCTCATT 
AATGACGGCG CGCTGGCGTG TGAGTGTGAT CCTCAAGGTT CGCTCAGCTC TGAGTGTGAT 
GTCCGTGGCG GTCAGTGCCG CTGCAGGCCC AACATATCTG GCCGGCGCTG TGAGAAATGT 
GCACCAGGAG CTTACGGCTT TGGCCCATCT GGATGCAAAC TTTGTGAATG CAGTTTAGAG 
GGCTCTCAGA GCCGGTTCTG TGACCTGTAC TCAGGACAGT GTCCATGCCG ACCTGGAGCA 
TATGGTCAGC GCTGTGATGG TTGTCAGGCA GGACACTGGG GTTTCCCAAA CTGCAGGCCA 
TGTCAATGTA ACGGCCATGC TGACGAGTGC CATCAGAGGA CTGGAGCCTG TCTCAACTGC 
AGAAGTAATA CTGCAGGAGA CAAATGTGAA AGGTGTGCTA ATGGCTACTA TGGTAACCCA 
GTCCTTGGTT TAGGGAGTCA GTGTCGGCCC TGCCCCTGTC CTGAGGGCCC CAACAGCGGA 
CGCCACTTTG CTGCCTCTTG TTACCAAGAC AACCGAAGCC AACAGGTGGT CTGCAACTGC 
AACCAGGGTT ACACAGGGAC TGTAAAGCGC TCTGCAGGGG CAGTCCTCAA TCGTCCACGT 
TGTGATGAAT GTGCTCCTGG CTATTTTGGT GACCCGTCTA AGCCCGGTGG GCAGTGCCAG 
CCATGTCGTT GCAGTAATAA CATCGACCTG TCAGATCCTG AGTCTTGTGA CAAGCGCACT 
GGCCAGTGTC TGAAGTGCCT GTACAACACT GAAGGGCCAG ACTGTAGTGT GTGCAGGAGC 
GGATACTATG GAGACGCTTC TCGACGCAAC TGCCGGAAGT GCACCTGTAA CTTCCTGGGC 
ACAGAGCGCA GTCAGTGTCT GTCTCGAGAT GACTGTGTGT GTCAGCGTGC CACTGGTCAG 
TGTCAGTGTC TGCCTAATGT TATTGGACAA ACCTGTGATC ACTGCGCTCC AAACTCCTGG 
AACCTGGCCA GCGGGCAAGG CTGTGAGCCC TGCGCATGTG ACCCTAACAA TGCCTACACA 
TCTGCCTGTA ATGAGTTTAC AGGCCAGTGC CAGTGCCGTG TTGGGTTTGG AGGAAAGACC 
TGTACAGACT GTCAGGAGAA CCACTGGGGT GACCCAAGAG TGCTATGCCG AGCATGTGAC 
TGTGACCCCC GTGGCATCGA GTCATCCCAA TGCAACCGTA TGACGGGTCA CTGCGTGTGC 
CAGCAGGGTG TTTCTGGTGT CCGCTGTGAT CAGTGTGCCC GTGGCTTCTC CGGGACCTTC 
CCTGACTGTA AGCCTTGCCA CCAGTGTTTT GGGGACTGGG ACCGTATCGT TCAGGATTTG 
GCCACCAGAA CTAAAGCCCT CACAGATCGA GCCAAGGAGC TTCAGACCAC CGGCCTGACT 
CGAGCATTCG AGAGACGCTT TAAGGAGCTG GAAGACATGC TGGCACAGGC ACGGGACATT 
GTTAATGCCC GTAATGCCAC TGCTGAGGCT GTGACCACCC TGATGGGCAT GATTGAGGTT 
CTCAGAGCTC AGATTGGCGA GACCACAGAC ACACTGAACC AGCAGGAGGG AGACCTGACC 
GCCGTGCAGG ACAGTAACTA TGAAGCCAGT AATGCTTTAA GCACACTGGA GAGAGAAGCA 
AAAGAGCTCA ACCTGAACGC AGATCAGCTT AACCGTCAGC TTGACATCCT CAAGAACTCC 
AACTTCTTGG GTGCATATGA CAGCATCCGG GCCTCGTATA ATAAGTCACG TGACGCAGAG 
CACCGATCAA ACAGATCTAC CACTGACACT CCCAGCACAG TCAGCCAATC AGCAGACACC 
CGCAAGAAAA CAGAGCGGCT GATTGGCCAA AAGAGAGATG ATTTTAACCG CAAGAACGCT 
GCTAACAAGC GTACACTCAC TGACTTAAAT GCAAAAGTTC AGAATCTGGA CTTAAAGAAA 
ATCAACGAAA AGGTGTGTGG AGCTCCAGGT GATGCTCAGT GTGTTGACAG TCCTTGTGGT 
GGTGCTGGTT GTCGTGATGA TGAAGGCAAG CGTCGATGTG GAGGCCTGAA CTGTAATGGA 
GCTGTTGCAG TGGCGGACAC TGCTCTGGAT AGATCCAAAC ATGCAGAAAA AGAGCTGGAT 
AAAGCAATGG GGGTAGTGGA GGAGCTTTTT AAGCAGGTGG CGGATGCTAA GACCAAGGCA 
CAGGAAGCGA AAGACAGAGC TCAGGCAGCT CTGGAAAAAG CTAGCGATAC CAAGAACAAA 
GTGGATCATT CTAACAATGA TCTAAGGGAC CTTATCAAAC AAATACGCCA ATTCCTCATG 
CAGGAGGGTG CAGATCCGGA CAGCATTGAG GCGGTGGCCA ATCGTGTGCT GGAGCTCTCA 
ATCCCTGCCT CTCCCAAGCA GATCCGACAC CTAGCAGACG AGATTAAAGA CCGTGTCAAG 
AGTCTGTCTA ATGTGGATGC CATTCTGGAG CAAACACAAA ATGACGTCCG CAAAGCAGAG 
CAACTGTTGC TGGATGCCAA GAAAGCTAGG AATAAGGCAG AGGGGGTGAA GAACACAGCT 
GAGAGTGTGA AAAAGGCCCT GAATGATGCC AGCAGAGCCC AGGCTGCAGC AGAGAAAGCC 
ATCCAGAAAG CTAAGAATGA CATTGGCTTA ACTCAGAACC AGCTGGCACA GATCCAGTCA 
GAGACCTCCG CTAGTGAGAG AGATCTTAAT GATGCAGTGG ACAGACTGGG AGACCTGGAG 
CGCCAGATCG AGGCATTAAA GACCAAACGT GCCAACAACA GCCTGGATGC TGCACGGGCA 
GAGGAGACGG CCACCATGGC ACGGGACAAA GCCAACGAAG CCAAAGAGAT CCTGGATGGT 
GAGCTGTCCG ATAAGTACCG TACTGTTCAG GGTCTGATGG ACAATAAGGC GAAAACCATG 
CAGGACGCCA AGCACAAAGC AGAGCAATTG AGAGATGAAG CCAAGGGGCT GCTGAAAGAT 
GCACAGGATA AACTGCAGAG GCTGGCAGAG CTTGAAAAGG ACTATGAAGA AAACCAGAAG 
GTATTGGAAG GCAAAGCCAG GCAGCTGGAT GGCCTGGAAG ATAAAATGAA GGCGATCCTG 
AACGCCATCA ACAGGCAGAT TCAGATCTAC AACACCTGTC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Canonical Wnt signalling regulates epithelial patterning by modulating levels of laminins in zebrafish appendages.
Development (Cambridge, England)142(2)320-30(2015 Jan)
Nagendran M,Arora P,Gori P,Mulay A,Ray S,Jacob T,Sonawane M

Thrombospondin-4 controls matrix assembly during development and repair of myotendinous junctions.
eLife3(2014 Jun)
Subramanian A,Schilling TF

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LAMB2 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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