mylipa cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	mylipa
Entrez Gene ID	335888
Full Name	myosin regulatory light chain interacting protein a
Synonyms	Mir,mylip,wu:fj36b03,zgc:55987
General protein information	Preferred Names myosin regulatory light chain interacting protein a Names E3 ubiquitin-protein ligase MYLIP-A MIR-A RING-type E3 ubiquitin transferase MYLIP-A myosin regulatory light chain-interacting protein A
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_199983.1	NP_956277.1	E3 ubiquitin-protein ligase MYLIP-A

Pathways from BioSystems

Homologies

Homo sapiens (human)	MYLIP	NP_037394.2
Bos taurus (cattle)	MYLIP	NP_001095723.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001609	XP_551203.4
Danio rerio (zebrafish)	mylipa	NP_956277.1
Xenopus tropicalis (tropical clawed frog)	mylip	NP_001017059.2
Mus musculus (house mouse)	Mylip	NP_722484.2
Gallus gallus (chicken)	MYLIP	NP_001012579.1
Drosophila melanogaster (fruit fly)	dnr1	NP_611680.2
Canis lupus familiaris (dog)	MYLIP	XP_545352.3
Pan troglodytes (chimpanzee)	MYLIP	XP_518252.2
	MYLIP	XP_001094408.1
Rattus norvegicus (Norway rat)	Mylip	NP_001100814.2

Gene Ontology
Bibliography

Related articles in PubMed

m
Zhao BS, Wang X, Beadell AV, Lu Z, Shi H, Kuuspalu A, Ho RK, He C
Nature542(7642)475-478(2017 02)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Mutant-specific gene expression profiling identifies SRY-related HMG box 11b (SOX11b) as a novel regulator of vascular development in zebrafish.
Schmitt CE, Woolls MJ, Jin SW
Molecules and cells35(2)166-72(2013 Feb)

Zebrafish Mir antagonizes Frizzled 7-induced gastrulation defects.
Knowlton MN, Kelly GM
Zebrafish1(2)133-44(2004)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

essential for embryonic development, with a role in the extensive cell movements associated with gastrulation
Title: The zebrafish band 4.1 member Mir is involved in cell movements associated with gastrulation.

The following mylipa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mylipa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa38402	NM_199983.1 Latest version!	Danio rerio myosin regulatory light chain interacting protein a (mylipa), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mylipa ( NM_199983.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956277.1 Danio rerio myosin regulatory light chain interacting protein a (mylipa), mRNA.

Price & Availability↑

CloneID	ODa38402
Clone ID Related Accession (Same CDS sequence)	NM_199983.1
Accession Version	NM_199983.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase MYLIP-A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY423020.1. ##Evidence-Data-START## Transcript exon combination :: AY423020.1, BC045844.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGCTCTGCC ATGTAACTCG ACCGGACGCG GTGGTGATGG AGATTGAAGT AGACGCGAAG 
GCGAACGGAG AAGACTGTCT GAATAAGGTA TGTCGGAAGT TGGGAATTAT AGAAGTGGAT 
TACTTTGGGC TGCAGTTCTC TGGCAGTAAA GGAGAAAACC TGTGGCTGAA CCTGAGGAAC 
AGAATCTCCC AGCAGATGGA CAATCTCACG CCCTGCAGAC TGCGGCTCCG AGTAAAGTTC 
TTTGTGGAGC CTCATCTTAT TCTGCAGGAG CAGACGAGGC ACCTGTTCTT CATGCATGTG 
AAAGAGGATC TGCACCGTGG GCATCTGCGC ATGTGCTCGG AACAAGCCCA AGAGCTCAGC 
GCACTCCTCG CACAGGCCGA GTTTGGGGAT TATAACCAGA ACACAGCCAA GTACTGGTAC 
ACTGAACTCT GCGGCTCAGA ACCAAACCAG ACAACCATCA ACAGTATCAT AGCCAAGCAC 
AAGGCTCTGG AGGGTCTGAG CCAGGCCTCG GTGGAGTATC AGGCTCTGCA GCTGGTGTCG 
TCACTGGAGC ATTATGGGGT GGAGTGGCAC TGGGCGCGGG ACGCTGAAGC ACAAAGACTG 
GCTATCGGTG TGGGTCCAGA GGGCATTGCT ATCTGTCGAG ATGATTTCAG CCTGGTCAAC 
AGGATATCCT ATCCCATCAT TCAAATCGCC ACGCAGTCAG GTAAGAGCGT ATACTTGACT 
GTCACCAAGG AGAGCAGCGA CAGTGTGGTC CTGCTGTTCA AGTTGATCAG TAATCGAGCC 
GCCAGTGGAC TTTACCGCGC CATCACGGAG ACACACGCTT TTTACAGATG TGACACAGTG 
ACCAACGCCG TCATGATGCA GTACAGCCGG GATTTCAAGG GTCACCTGGC TTCACTTTTC 
CTCAACGAGA ACATCAACCT GGGGAAGAAG TACGTATTTG ATATCCGCCG CACATCTAAG 
GAAGTGTACG ACTACGCCAG ACGCGCACTT TACAATGCGG GGATCGTGGA CATGATGGCT 
CGGCCGGGAG AGCGCACGCC ATCCAACCGC TCTCCGTCCC GGGAGCAGGA GGGTGCGCTG 
GACTGTGGGG GCTGCCAGCA GAGCCGCCTG CTGCAGGAGA AGCTGCAGAA GCTGAGGGAG 
GCTCTGCTCT GTATGCTGTG CTGCGAGGAG GAGATTGACG CCGCCTTCTG CCCCTGCGGA 
CACATGGTCT GCTGCCAGAA CTGTGCTGCT CAGTTGCAGT CATGCCCAGT TTGTCGCTCA 
GAAGTCGAAC ATGTCCAACA TGTCTACTTG CCCACATGTA CCAGTCTACT AAACCTGACC 
ATTGGCGAAA ACAGCCCCGA GCCCATCCAT CGAGGCATGG CGGCCCATAC CTGCACTACC 
AACGACTACT CCACCAGCGA GAAAATCTAC CAAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956277.1
CDS	148..1566
Translation	MLCHVTRPDAVVMEIEVDAKANGEDCLNKVCRKLGIIEVDYFGLQFSGSKGENLWLNLRNRISQQMDNLTPCRLRLRVKFFVEPHLILQEQTRHLFFMHVKEDLHRGHLRMCSEQAQELSALLAQAEFGDYNQNTAKYWYTELCGSEPNQTTINSIIAKHKALEGLSQASVEYQALQLVSSLEHYGVEWHWARDAEAQRLAIGVGPEGIAICRDDFSLVNRISYPIIQIATQSGKSVYLTVTKESSDSVVLLFKLISNRAASGLYRAITETHAFYRCDTVTNAVMMQYSRDFKGHLASLFLNENINLGKKYVFDIRRTSKEVYDYARRALYNAGIVDMMARPGERTPSNRSPSREQEGALDCGGCQQSRLLQEKLQKLREALLCMLCCEEEIDAAFCPCGHMVCCQNCAAQLQSCPVCRSEVEHVQHVYLPTCTSLLNLTIGENSPEPIHRGMAAHTCTTNDYSTSEKIYQN

Target ORF information:

RefSeq Version	NM_199983.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myosin regulatory light chain interacting protein a (mylipa), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199983.1

ORF Insert Sequence:

ATGCTCTGCC ATGTAACTCG ACCGGACGCG GTGGTGATGG AGATTGAAGT AGACGCGAAG 
GCGAACGGAG AAGACTGTCT GAATAAGGTA TGTCGGAAGT TGGGAATTAT AGAAGTGGAT 
TACTTTGGGC TGCAGTTCTC TGGCAGTAAA GGAGAAAACC TGTGGCTGAA CCTGAGGAAC 
AGAATCTCCC AGCAGATGGA CAATCTCACG CCCTGCAGAC TGCGGCTCCG AGTAAAGTTC 
TTTGTGGAGC CTCATCTTAT TCTGCAGGAG CAGACGAGGC ACCTGTTCTT CATGCATGTG 
AAAGAGGATC TGCACCGTGG GCATCTGCGC ATGTGCTCGG AACAAGCCCA AGAGCTCAGC 
GCACTCCTCG CACAGGCCGA GTTTGGGGAT TATAACCAGA ACACAGCCAA GTACTGGTAC 
ACTGAACTCT GCGGCTCAGA ACCAAACCAG ACAACCATCA ACAGTATCAT AGCCAAGCAC 
AAGGCTCTGG AGGGTCTGAG CCAGGCCTCG GTGGAGTATC AGGCTCTGCA GCTGGTGTCG 
TCACTGGAGC ATTATGGGGT GGAGTGGCAC TGGGCGCGGG ACGCTGAAGC ACAAAGACTG 
GCTATCGGTG TGGGTCCAGA GGGCATTGCT ATCTGTCGAG ATGATTTCAG CCTGGTCAAC 
AGGATATCCT ATCCCATCAT TCAAATCGCC ACGCAGTCAG GTAAGAGCGT ATACTTGACT 
GTCACCAAGG AGAGCAGCGA CAGTGTGGTC CTGCTGTTCA AGTTGATCAG TAATCGAGCC 
GCCAGTGGAC TTTACCGCGC CATCACGGAG ACACACGCTT TTTACAGATG TGACACAGTG 
ACCAACGCCG TCATGATGCA GTACAGCCGG GATTTCAAGG GTCACCTGGC TTCACTTTTC 
CTCAACGAGA ACATCAACCT GGGGAAGAAG TACGTATTTG ATATCCGCCG CACATCTAAG 
GAAGTGTACG ACTACGCCAG ACGCGCACTT TACAATGCGG GGATCGTGGA CATGATGGCT 
CGGCCGGGAG AGCGCACGCC ATCCAACCGC TCTCCGTCCC GGGAGCAGGA GGGTGCGCTG 
GACTGTGGGG GCTGCCAGCA GAGCCGCCTG CTGCAGGAGA AGCTGCAGAA GCTGAGGGAG 
GCTCTGCTCT GTATGCTGTG CTGCGAGGAG GAGATTGACG CCGCCTTCTG CCCCTGCGGA 
CACATGGTCT GCTGCCAGAA CTGTGCTGCT CAGTTGCAGT CATGCCCAGT TTGTCGCTCA 
GAAGTCGAAC ATGTCCAACA TGTCTACTTG CCCACATGTA CCAGTCTACT AAACCTGACC 
ATTGGCGAAA ACAGCCCCGA GCCCATCCAT CGAGGCATGG CGGCCCATAC CTGCACTACC 
AACGACTACT CCACCAGCGA GAAAATCTAC CAAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

m
Nature542(7642)475-478(2017 02)
Zhao BS,Wang X,Beadell AV,Lu Z,Shi H,Kuuspalu A,Ho RK,He C

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Mutant-specific gene expression profiling identifies SRY-related HMG box 11b (SOX11b) as a novel regulator of vascular development in zebrafish.
Molecules and cells35(2)166-72(2013 Feb)
Schmitt CE,Woolls MJ,Jin SW

Zebrafish Mir antagonizes Frizzled 7-induced gastrulation defects.
Zebrafish1(2)133-44(2004)
Knowlton MN,Kelly GM