SRSF5A cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	SRSF5A
Entrez Gene ID	335396
Full Name	serine/arginine-rich splicing factor 5a
Synonyms	im:6906390,sfrs5,sfrs5a,wu:fb49e01,wu:fj09b12,zgc:77797
General protein information	Preferred Names serine/arginine-rich splicing factor 5a Names serine/arginine-rich splicing factor 5a fb49e01 splicing factor, arginine/serine-rich 5 splicing factor, arginine/serine-rich 5a
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_200867.1	NP_957161.1	serine and arginine rich splicing factor 5a
XM_005156982.4	XP_005157039.1	serine/arginine-rich splicing factor 5a isoform X1
XM_005156982.3	XP_005157039.1	serine/arginine-rich splicing factor 5a isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	srsf5a	NP_957161.1
Xenopus tropicalis (tropical clawed frog)	krt5.1	NP_001005638.1
Drosophila melanogaster (fruit fly)	B52	NP_788665.1

Gene Ontology
Bibliography

Related articles in PubMed

Number of inadvertent RNA targets for morpholino knockdown in Danio rerio is largely underestimated: evidence from the study of Ser/Arg-rich splicing factors.
Joris M, Schloesser M, Baurain D, Hanikenne M, Muller M, Motte P
Nucleic acids research45(16)9547-9557(2017 Sep)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

A transgenic zebrafish model expressing KIT-D816V recapitulates features of aggressive systemic mastocytosis.
Balci TB, Prykhozhij SV, Teh EM, Da'as SI, McBride E, Liwski R, Chute IC, Leger D, Lewis SM, Berman JN
British journal of haematology167(1)48-61(2014 Oct)

The earliest transcribed zygotic genes are short, newly evolved, and different across species.
Heyn P, Kircher M, Dahl A, Kelso J, Tomancak P, Kalinka AT, Neugebauer KM
Cell reports6(2)285-92(2014 Jan)

Genomewide expression analysis in zebrafish mind bomb alleles with pancreas defects of different severity identifies putative Notch responsive genes.
Hegde A, Qiu NC, Qiu X, Ho SH, Tay KQ, George J, Ng FS, Govindarajan KR, Gong Z, Mathavan S, Jiang YJ
PloS one3(1)e1479(2008 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SRSF5A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SRSF5A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa01950	NM_200867.1 Latest version!	Danio rerio serine and arginine rich splicing factor 5a (srsf5a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
ODa18973	XM_005156982.4 Latest version!	Danio rerio serine/arginine-rich splicing factor 5a (srsf5a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
View Old Accession Versions >>
ODa18973	XM_005156982.3	Danio rerio serine/arginine-rich splicing factor 5a (srsf5a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SRSF5A ( NM_200867.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_957161.1 Danio rerio serine and arginine rich splicing factor 5a (srsf5a), mRNA.

Price & Availability↑

CloneID	ODa01950
Clone ID Related Accession (Same CDS sequence)	NM_200867.1
Accession Version	NM_200867.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-07
Organism	Danio rerio(zebrafish)
Product	serine and arginine rich splicing factor 5a
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY398417.1. ##Evidence-Data-START## Transcript exon combination :: AY398417.1, CN015216.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476732, SAMEA4476735 [ECO:0000348] ##Evidence-Data-END##

ATGAGTGGAT GTCGTGTGTT TATTGGCCGT CTGAGCCCTC ATGCTCGTGA AAGAGATGTG 
GAGAAGTTCT TCAAAGGCTA CGGACGTATC CGAGAGATTA ACCTGAAGAA CGGGTTTGGC 
TTTGTGGAGT TTGACGACTA CAGGGATGCA GATGATGCAG TGTATGAACT GAATGGCAAA 
GAGCTGTGCA GTGAGAGGGT GACCATTGAG CATGCCCGCT CTCGCAGAGG AAGAGGTGGT 
GGCCCAGGAA TGGGAGGTCG TTTCTCTCCA CGCTTTGGAG GTTATCGTCA GTCCCGCAGT 
GGCGGTTCCA GGTATGGACC ACCAGTGCGC ACAGAGCACA GAATCATAGT GGAGAATCTC 
TCCTCTCGCA TCAGCTGGCA GGACCTGAAG GACTTGATGA GGAAGGTTGG AGAAGTAACT 
TTTGTGGATG CACACAGGAC CAAAAAGAAT GAAGGGGTGG TGGAGTTTGC TTCACACAGT 
GATATGAAGA ATGCCATCGA GAAGCTTGAT GGAACTGACC TGAACGGGCG CAAACTCAAG 
TTGTATGAGG ACCGCAAGAG GAACCGCAGT AGGAGTCGTT CCCGCAGCAG GAGCAGCTCT 
CGTTCTCGTT CCCCAAGCCG CAGTCGCAGC CCCGAGCGTG GAAGCAAGCG CTCTCGCAGC 
CGCTCCCTCA GTCGCACCCC AGAGAAGAAA TCCACCAGCA ACAGATCTCC TTCCCGCTCT 
CCAACTCCTC AGAGGAAACA GAGCCGCTCT CGATCTGCCT CCGCAGAGAG TCAGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_957161.1
CDS	43..822
Translation	MSGCRVFIGRLSPHARERDVEKFFKGYGRIREINLKNGFGFVEFDDYRDADDAVYELNGKELCSERVTIEHARSRRGRGGGPGMGGRFSPRFGGYRQSRSGGSRYGPPVRTEHRIIVENLSSRISWQDLKDLMRKVGEVTFVDAHRTKKNEGVVEFASHSDMKNAIEKLDGTDLNGRKLKLYEDRKRNRSRSRSRSRSSSRSRSPSRSRSPERGSKRSRSRSLSRTPEKKSTSNRSPSRSPTPQRKQSRSRSASAESQH

Target ORF information:

RefSeq Version	NM_200867.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio serine and arginine rich splicing factor 5a (srsf5a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200867.1

ORF Insert Sequence:

ATGAGTGGAT GTCGTGTGTT TATTGGCCGT CTGAGCCCTC ATGCTCGTGA AAGAGATGTG 
GAGAAGTTCT TCAAAGGCTA CGGACGTATC CGAGAGATTA ACCTGAAGAA CGGGTTTGGC 
TTTGTGGAGT TTGACGACTA CAGGGATGCA GATGATGCAG TGTATGAACT GAATGGCAAA 
GAGCTGTGCA GTGAGAGGGT GACCATTGAG CATGCCCGCT CTCGCAGAGG AAGAGGTGGT 
GGCCCAGGAA TGGGAGGTCG TTTCTCTCCA CGCTTTGGAG GTTATCGTCA GTCCCGCAGT 
GGCGGTTCCA GGTATGGACC ACCAGTGCGC ACAGAGCACA GAATCATAGT GGAGAATCTC 
TCCTCTCGCA TCAGCTGGCA GGACCTGAAG GACTTGATGA GGAAGGTTGG AGAAGTAACT 
TTTGTGGATG CACACAGGAC CAAAAAGAAT GAAGGGGTGG TGGAGTTTGC TTCACACAGT 
GATATGAAGA ATGCCATCGA GAAGCTTGAT GGAACTGACC TGAACGGGCG CAAACTCAAG 
TTGTATGAGG ACCGCAAGAG GAACCGCAGT AGGAGTCGTT CCCGCAGCAG GAGCAGCTCT 
CGTTCTCGTT CCCCAAGCCG CAGTCGCAGC CCCGAGCGTG GAAGCAAGCG CTCTCGCAGC 
CGCTCCCTCA GTCGCACCCC AGAGAAGAAA TCCACCAGCA ACAGATCTCC TTCCCGCTCT 
CCAACTCCTC AGAGGAAACA GAGCCGCTCT CGATCTGCCT CCGCAGAGAG TCAGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SRSF5A ( XM_005156982.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005157039.1 Danio rerio serine/arginine-rich splicing factor 5a (srsf5a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa18973
Clone ID Related Accession (Same CDS sequence)	XM_005156982.4 , XM_005156982.3
Accession Version	XM_005156982.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/arginine-rich splicing factor 5a isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005156982.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGGAT GTCGTGTGTT TATTGGCCGT CTGAGCCCTC ATGCTCGTGA AAGAGATGTG 
GAGAAGTTCT TCAAAGGCTA CGGACGTATC CGAGAGATTA ACCTGAAAAA CGGGTTTGGC 
TTTGTGGAGT TTGACGACTA CAGGGATGCA GATGATGCAG TGTATGAACT GAATGGCAAA 
GAGCTGTGCA GTGAGAGGGT GACCATTGAG CATGCCCGCT CTCGCAGAGG AAGAGGTGGT 
GGCCCAGGAA TGGGAGGTCG TTTCTCTCCA CGCTTTGGAG GTTATCGTCA GTCCCGCAGT 
GGCGGTTCCA GGTATGGACC ACCAGTGCGC ACAGAGCACA GAATCATAGT GGAGAATCTC 
TCCTCTCGCA TCAGCTGGCA GCTGTGCTCT GGTATTCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157039.1
CDS	127..528
Translation	MSGCRVFIGRLSPHARERDVEKFFKGYGRIREINLKNGFGFVEFDDYRDADDAVYELNGKELCSERVTIEHARSRRGRGGGPGMGGRFSPRFGGYRQSRSGGSRYGPPVRTEHRIIVENLSSRISWQLCSGIL

Target ORF information:

RefSeq Version	XM_005156982.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio serine/arginine-rich splicing factor 5a (srsf5a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156982.4

ORF Insert Sequence:

ATGAGTGGAT GTCGTGTGTT TATTGGCCGT CTGAGCCCTC ATGCTCGTGA AAGAGATGTG 
GAGAAGTTCT TCAAAGGCTA CGGACGTATC CGAGAGATTA ACCTGAAAAA CGGGTTTGGC 
TTTGTGGAGT TTGACGACTA CAGGGATGCA GATGATGCAG TGTATGAACT GAATGGCAAA 
GAGCTGTGCA GTGAGAGGGT GACCATTGAG CATGCCCGCT CTCGCAGAGG AAGAGGTGGT 
GGCCCAGGAA TGGGAGGTCG TTTCTCTCCA CGCTTTGGAG GTTATCGTCA GTCCCGCAGT 
GGCGGTTCCA GGTATGGACC ACCAGTGCGC ACAGAGCACA GAATCATAGT GGAGAATCTC 
TCCTCTCGCA TCAGCTGGCA GCTGTGCTCT GGTATTCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SRSF5A ( XM_005156982.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005157039.1 Danio rerio serine/arginine-rich splicing factor 5a (srsf5a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa18973
Clone ID Related Accession (Same CDS sequence)	XM_005156982.4 , XM_005156982.3
Accession Version	XM_005156982.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	serine/arginine-rich splicing factor 5a isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005156982.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGGAT GTCGTGTGTT TATTGGCCGT CTGAGCCCTC ATGCTCGTGA AAGAGATGTG 
GAGAAGTTCT TCAAAGGCTA CGGACGTATC CGAGAGATTA ACCTGAAAAA CGGGTTTGGC 
TTTGTGGAGT TTGACGACTA CAGGGATGCA GATGATGCAG TGTATGAACT GAATGGCAAA 
GAGCTGTGCA GTGAGAGGGT GACCATTGAG CATGCCCGCT CTCGCAGAGG AAGAGGTGGT 
GGCCCAGGAA TGGGAGGTCG TTTCTCTCCA CGCTTTGGAG GTTATCGTCA GTCCCGCAGT 
GGCGGTTCCA GGTATGGACC ACCAGTGCGC ACAGAGCACA GAATCATAGT GGAGAATCTC 
TCCTCTCGCA TCAGCTGGCA GCTGTGCTCT GGTATTCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157039.1
CDS	128..529
Translation	MSGCRVFIGRLSPHARERDVEKFFKGYGRIREINLKNGFGFVEFDDYRDADDAVYELNGKELCSERVTIEHARSRRGRGGGPGMGGRFSPRFGGYRQSRSGGSRYGPPVRTEHRIIVENLSSRISWQLCSGIL

Target ORF information:

RefSeq Version	XM_005156982.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio serine/arginine-rich splicing factor 5a (srsf5a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156982.3

ORF Insert Sequence:

ATGAGTGGAT GTCGTGTGTT TATTGGCCGT CTGAGCCCTC ATGCTCGTGA AAGAGATGTG 
GAGAAGTTCT TCAAAGGCTA CGGACGTATC CGAGAGATTA ACCTGAAAAA CGGGTTTGGC 
TTTGTGGAGT TTGACGACTA CAGGGATGCA GATGATGCAG TGTATGAACT GAATGGCAAA 
GAGCTGTGCA GTGAGAGGGT GACCATTGAG CATGCCCGCT CTCGCAGAGG AAGAGGTGGT 
GGCCCAGGAA TGGGAGGTCG TTTCTCTCCA CGCTTTGGAG GTTATCGTCA GTCCCGCAGT 
GGCGGTTCCA GGTATGGACC ACCAGTGCGC ACAGAGCACA GAATCATAGT GGAGAATCTC 
TCCTCTCGCA TCAGCTGGCA GCTGTGCTCT GGTATTCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Number of inadvertent RNA targets for morpholino knockdown in Danio rerio is largely underestimated: evidence from the study of Ser/Arg-rich splicing factors.
Nucleic acids research45(16)9547-9557(2017 Sep)
Joris M,Schloesser M,Baurain D,Hanikenne M,Muller M,Motte P

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

A transgenic zebrafish model expressing KIT-D816V recapitulates features of aggressive systemic mastocytosis.
British journal of haematology167(1)48-61(2014 Oct)
Balci TB,Prykhozhij SV,Teh EM,Da'as SI,McBride E,Liwski R,Chute IC,Leger D,Lewis SM,Berman JN

The earliest transcribed zygotic genes are short, newly evolved, and different across species.
Cell reports6(2)285-92(2014 Jan)
Heyn P,Kircher M,Dahl A,Kelso J,Tomancak P,Kalinka AT,Neugebauer KM

Genomewide expression analysis in zebrafish mind bomb alleles with pancreas defects of different severity identifies putative Notch responsive genes.
PloS one3(1)e1479(2008 Jan)
Hegde A,Qiu NC,Qiu X,Ho SH,Tay KQ,George J,Ng FS,Govindarajan KR,Gong Z,Mathavan S,Jiang YJ

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SRSF5A cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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