taf3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	taf3
Entrez Gene ID	334474
Full Name	TAF3 RNA polymerase II, TATA box binding protein (TBP)-associated facto
Synonyms	fi76b06,wu:fi76b06,zgc:136280
General protein information	Preferred Names TAF3 RNA polymerase II, TATA box binding protein (TBP)-associated facto Names transcription initiation factor TFIID subunit 3 TAF3 RNA polymerase II, TATA box binding protein (TBP)-associated factor
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001042744.2	NP_001036209.1	transcription initiation factor TFIID subunit 3

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

The basal transcription complex component TAF3 transduces changes in nuclear phosphoinositides into transcriptional output.
Stijf-Bultsma Y, Sommer L, Tauber M, Baalbaki M, Giardoglou P, Jones DR, Gelato KA, van Pelt J, Shah Z, Rahnamoun H, Toma C, Anderson KE, Hawkins P, Lauberth SM, Haramis AP, Hart D, Fischle W, Divecha N
Molecular cell58(3)453-67(2015 May)

Quantitative mass spectrometry of TATA binding protein-containing complexes and subunit phosphorylations during the cell cycle.
Pijnappel WP, Kolkman A, Baltissen MP, Heck AJ, Timmers HM
Proteome science746(2009 Dec)

Selective interaction between Trf3 and Taf3 required for early development and hematopoiesis.
Hart DO, Santra MK, Raha T, Green MR
Developmental dynamics : an official publication of the American Association of Anatomists238(10)2540-9(2009 Oct)

The TFIID components human TAF(II)140 and Drosophila BIP2 (TAF(II)155) are novel metazoan homologues of yeast TAF(II)47 containing a histone fold and a PHD finger.
Gangloff YG, Pointud JC, Thuault S, Carr? L, Romier C, Muratoglu S, Brand M, Tora L, Couderc JL, Davidson I
Molecular and cellular biology21(15)5109-21(2001 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that like Trf3-depleted zebrafish embryos, Taf3-depleted embryos exhibit multiple developmental defects and fail to undergo hematopoiesis.
Title: Selective interaction between Trf3 and Taf3 required for early development and hematopoiesis.

The following taf3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the taf3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa31051	NM_001042744.2 Latest version!	Danio rerio TAF3 RNA polymerase II, TATA box binding protein (TBP)-associated facto (taf3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

taf3 ( NM_001042744.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001036209.1 Danio rerio TAF3 RNA polymerase II, TATA box binding protein (TBP)-associated facto (taf3), mRNA.

Price & Availability↑

CloneID	ODa31051
Clone ID Related Accession (Same CDS sequence)	NM_001042744.2
Accession Version	NM_001042744.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2697bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	transcription initiation factor TFIID subunit 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX640594.5 and BX927352.15. On May 28, 2010 this sequence version replaced NM_001042744.1. ##Evidence-Data-START## Transcript exon combination :: GDQH01012965.1, GDQQ01013476.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTGTGAGA GCTTCGCGCG CTCTCTGCTG CGGGTCTCCG TGGCCCAGAT GTGTCAGGCT 
GTAGGCTGGG ACGCGGTGCA GCTGAGCGCC TGCGATCTGC TCTCCGACGT GCTGGAGAGA 
TACGTGCAGC AGCTGGCCAA GAGCTGCCAC CGTTACTCTG AGCTCTATGG ACGTACAGAC 
CCTGGATTGA GTGATGTAGA CCAGGCTTTT GGGTTTCTGG GAGTCAGTAT AGCTGAACTT 
GAGGACTATG TAAATAATGT TGAGCCTATC GGTTACCCAC AAACAATCCC TCAGTTCCCC 
ATCAGCAAGA GCAGCGTTCT GCAGTTTCCC GCCGCAGGCT TCGACACTGA TGCCCGCGAC 
GCTCTCAGGG GTGAGAGGAG AGACTACATC CCAGAATACT TCCCTCCACT TGTCTCCCTG 
CAGGAAGATG AGGAAGAGGA GGAACCAGTT CCTGCAGACA TGGGCACTTC AGCTGAGGCC 
ATGCAGGTCT CCATGGGTGA GGAAGAGGAT GGTGAGGAAG AGGAAAATGA TGAAAACTGG 
CTTAGTCGTC ATGATGGACA GAGTCCCAGA CCGGAGGGGT TATTACCCGC CGCTAAGAGA 
CCTCGCCTTA GCACCAAACC CGGCGTTAGT CCAGAATGGA GTTATGAACC TCGGGAGCCC 
CTCAGTTCAC TCAACTCCCA ACACATGCCC CATTCACCCT CCCCTCCGCT CTCCCCCGAA 
TTACCCCGAC CTCCTGTGGC ACCGCCTACA CCTGGACACA AGCCCAAACT GCCATCACCT 
TCTCCTGCCC GGCAGAAGAA CAAGTCACCC AAAAGAGGGG GTGCTGCACC CACTGGTGTA 
TTGGGGCGAC CACTTACATT GACCCCACCC AAGACTAATC AGAAAGCAGG AAAGAGATCA 
CCTGGTATGC CCAAAAGTCC TCGCAGCCCA AGGCCAAGTT CTGCCCCTTT GGGCATAGCT 
GGGCGTGGTT GGCCTGGACC TCTTGCAGAT GATAGTTCTC GTGATGGACA GTTTAAATGT 
AATGACACAG ATGACAAGGA TATTGAGAAC TCTATTGCTG CTGTAATCGC TCGCGCTTGT 
GCAGGTGAAG ATGGCACATC AGACCCATTT GCATACGACT CTGACTCAGA CAGTGAGGGT 
CCACACCCTC CAAAACCTGT GCCCAAACAT ACACTAAGCC ACATCAAACC TCCACAAGCT 
CTCACTAGTA AACACCTGCT TCCACCAGTT CCCCCACTTC TTAACCCGCC TATCCGTTGG 
ACAATGGATG ACTCGATAGA TGAGGTCATT CGAAAGGCCA GTCAGGGACA AAAGCCAATT 
CAACCCCCAA GTCTTTCCCC GCTAGAGCCC CCTTACCTGT CATCACCATC TGACTCACCT 
CCCACACCGC CACCACCCCT CAAGCTGACC CAAGAAAGGA AAGATTCACC TACTGGTAAT 
CTTCTGAAAA AGAAACTCAA GAAGGACATC AAGACCAAGT TAAAGAAGAA AGACAAGGAG 
CGACTCAAGG AGAAGGGCAA AGAAAAAGGA AAGAACAAGG AGAAAAATAA AGAGAAGAAG 
CGGGAAAAGG AGAGAGGGCA GGTGGAGGAG AACAAGATGC CCTGGCCGGA GCTATCGCTT 
GTAGTCGGCG GAGAAGAGCG GAGAGATGGC ATTGGCGGGA AGAAAGAGAA GGACAAGCAC 
AAGGACAAGA AGAAAGATAA AGAGAAAGGC AAGAAAGAGA AAGAGAAACG AGACAAGGGC 
AAGGAGCGGG GCAAGGAAGA GAAAAGGATA TCTCAAGGAA CCAAAGACAT TGCCATTGCC 
GTCGCCGGTG CCACCAGCCC ACTCTTCAGC CCCCAGACCT GCTTAAGAAT CCCATCAATG 
TTGCCCCCTT TGCCACCAAT ACTCCCTGAT AAGTTGTTCC CACACCCCAA GGATATAAAG 
AGCAAAGACA AGGACAAGAA GAAGGATAAG AAAGAGAAGA AGAAGAAAAA AGAGAAGGAG 
AAAGATCGAG AGAAGGAGCG GGAGAAGGAA CGGGAGAAGG AACGCACAAA GGAGAAGGAG 
CGAGATAAAG AGGAGAAGAA GAAAGACAAA GAGAAGAAGG AAAAGACAAA GGAAAAGGAG 
AAAGACAAGC CAAAATTAGA GAAAACTAGT CTAATGGCTG AAATTCCTCC TGCTGTCCCA 
CCGTCTCCTG TCATACCCCG ACTGACCCTC AGAGTGGGCG CCGGACAAGA CAAGATTGTG 
ATCAGTAAAG TGGTGCCAGA TACAGAGCCC ACTCCTCCTC CACCGCGGAC CCCAGTGGCC 
CGAATGGGTC CCGGAGCCCG AATACGTCCT CCTCCAGTTC TTCCTCCACC TGCCCCGCCT 
CCTGCAACCC CTCCACCTCG TCCTCCACCC GTGCTGCCTC CACCTCCACC TCCAGCGGCC 
CCCATCCAGA CTCCACCCAA CCAGGCTAAG GCTCGTGGCT GCAGCGTCGT CACCGAGACT 
GTCAGTGCTT ATGTGATTCG GGATGAATGG GGTAATCAGA TCTGGATTTG TCCAGGATGT 
AATAAAGCTG ATGACGGTAG TCCAATGATC GGATGTGATG AGTGCGATGA CTGGTATCAC 
TGGCCTTGTG TGGGTCTTCT TGCCGCTCCT CCCGAGGACC AGTCCTGGTT TTGCATAAAA 
TGTGCTGGCA AGAAGAAAGA CAAGAAGACA AAAAAAAGGA AACGCAAAGC GCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036209.1
CDS	1..2697
Translation	MCESFARSLLRVSVAQMCQAVGWDAVQLSACDLLSDVLERYVQQLAKSCHRYSELYGRTDPGLSDVDQAFGFLGVSIAELEDYVNNVEPIGYPQTIPQFPISKSSVLQFPAAGFDTDARDALRGERRDYIPEYFPPLVSLQEDEEEEEPVPADMGTSAEAMQVSMGEEEDGEEEENDENWLSRHDGQSPRPEGLLPAAKRPRLSTKPGVSPEWSYEPREPLSSLNSQHMPHSPSPPLSPELPRPPVAPPTPGHKPKLPSPSPARQKNKSPKRGGAAPTGVLGRPLTLTPPKTNQKAGKRSPGMPKSPRSPRPSSAPLGIAGRGWPGPLADDSSRDGQFKCNDTDDKDIENSIAAVIARACAGEDGTSDPFAYDSDSDSEGPHPPKPVPKHTLSHIKPPQALTSKHLLPPVPPLLNPPIRWTMDDSIDEVIRKASQGQKPIQPPSLSPLEPPYLSSPSDSPPTPPPPLKLTQERKDSPTGNLLKKKLKKDIKTKLKKKDKERLKEKGKEKGKNKEKNKEKKREKERGQVEENKMPWPELSLVVGGEERRDGIGGKKEKDKHKDKKKDKEKGKKEKEKRDKGKERGKEEKRISQGTKDIAIAVAGATSPLFSPQTCLRIPSMLPPLPPILPDKLFPHPKDIKSKDKDKKKDKKEKKKKKEKEKDREKEREKEREKERTKEKERDKEEKKKDKEKKEKTKEKEKDKPKLEKTSLMAEIPPAVPPSPVIPRLTLRVGAGQDKIVISKVVPDTEPTPPPPRTPVARMGPGARIRPPPVLPPPAPPPATPPPRPPPVLPPPPPPAAPIQTPPNQAKARGCSVVTETVSAYVIRDEWGNQIWICPGCNKADDGSPMIGCDECDDWYHWPCVGLLAAPPEDQSWFCIKCAGKKKDKKTKKRKRKAH

Target ORF information:

RefSeq Version	NM_001042744.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio TAF3 RNA polymerase II, TATA box binding protein (TBP)-associated facto (taf3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042744.2

ORF Insert Sequence:

ATGTGTGAGA GCTTCGCGCG CTCTCTGCTG CGGGTCTCCG TGGCCCAGAT GTGTCAGGCT 
GTAGGCTGGG ACGCGGTGCA GCTGAGCGCC TGCGATCTGC TCTCCGACGT GCTGGAGAGA 
TACGTGCAGC AGCTGGCCAA GAGCTGCCAC CGTTACTCTG AGCTCTATGG ACGTACAGAC 
CCTGGATTGA GTGATGTAGA CCAGGCTTTT GGGTTTCTGG GAGTCAGTAT AGCTGAACTT 
GAGGACTATG TAAATAATGT TGAGCCTATC GGTTACCCAC AAACAATCCC TCAGTTCCCC 
ATCAGCAAGA GCAGCGTTCT GCAGTTTCCC GCCGCAGGCT TCGACACTGA TGCCCGCGAC 
GCTCTCAGGG GTGAGAGGAG AGACTACATC CCAGAATACT TCCCTCCACT TGTCTCCCTG 
CAGGAAGATG AGGAAGAGGA GGAACCAGTT CCTGCAGACA TGGGCACTTC AGCTGAGGCC 
ATGCAGGTCT CCATGGGTGA GGAAGAGGAT GGTGAGGAAG AGGAAAATGA TGAAAACTGG 
CTTAGTCGTC ATGATGGACA GAGTCCCAGA CCGGAGGGGT TATTACCCGC CGCTAAGAGA 
CCTCGCCTTA GCACCAAACC CGGCGTTAGT CCAGAATGGA GTTATGAACC TCGGGAGCCC 
CTCAGTTCAC TCAACTCCCA ACACATGCCC CATTCACCCT CCCCTCCGCT CTCCCCCGAA 
TTACCCCGAC CTCCTGTGGC ACCGCCTACA CCTGGACACA AGCCCAAACT GCCATCACCT 
TCTCCTGCCC GGCAGAAGAA CAAGTCACCC AAAAGAGGGG GTGCTGCACC CACTGGTGTA 
TTGGGGCGAC CACTTACATT GACCCCACCC AAGACTAATC AGAAAGCAGG AAAGAGATCA 
CCTGGTATGC CCAAAAGTCC TCGCAGCCCA AGGCCAAGTT CTGCCCCTTT GGGCATAGCT 
GGGCGTGGTT GGCCTGGACC TCTTGCAGAT GATAGTTCTC GTGATGGACA GTTTAAATGT 
AATGACACAG ATGACAAGGA TATTGAGAAC TCTATTGCTG CTGTAATCGC TCGCGCTTGT 
GCAGGTGAAG ATGGCACATC AGACCCATTT GCATACGACT CTGACTCAGA CAGTGAGGGT 
CCACACCCTC CAAAACCTGT GCCCAAACAT ACACTAAGCC ACATCAAACC TCCACAAGCT 
CTCACTAGTA AACACCTGCT TCCACCAGTT CCCCCACTTC TTAACCCGCC TATCCGTTGG 
ACAATGGATG ACTCGATAGA TGAGGTCATT CGAAAGGCCA GTCAGGGACA AAAGCCAATT 
CAACCCCCAA GTCTTTCCCC GCTAGAGCCC CCTTACCTGT CATCACCATC TGACTCACCT 
CCCACACCGC CACCACCCCT CAAGCTGACC CAAGAAAGGA AAGATTCACC TACTGGTAAT 
CTTCTGAAAA AGAAACTCAA GAAGGACATC AAGACCAAGT TAAAGAAGAA AGACAAGGAG 
CGACTCAAGG AGAAGGGCAA AGAAAAAGGA AAGAACAAGG AGAAAAATAA AGAGAAGAAG 
CGGGAAAAGG AGAGAGGGCA GGTGGAGGAG AACAAGATGC CCTGGCCGGA GCTATCGCTT 
GTAGTCGGCG GAGAAGAGCG GAGAGATGGC ATTGGCGGGA AGAAAGAGAA GGACAAGCAC 
AAGGACAAGA AGAAAGATAA AGAGAAAGGC AAGAAAGAGA AAGAGAAACG AGACAAGGGC 
AAGGAGCGGG GCAAGGAAGA GAAAAGGATA TCTCAAGGAA CCAAAGACAT TGCCATTGCC 
GTCGCCGGTG CCACCAGCCC ACTCTTCAGC CCCCAGACCT GCTTAAGAAT CCCATCAATG 
TTGCCCCCTT TGCCACCAAT ACTCCCTGAT AAGTTGTTCC CACACCCCAA GGATATAAAG 
AGCAAAGACA AGGACAAGAA GAAGGATAAG AAAGAGAAGA AGAAGAAAAA AGAGAAGGAG 
AAAGATCGAG AGAAGGAGCG GGAGAAGGAA CGGGAGAAGG AACGCACAAA GGAGAAGGAG 
CGAGATAAAG AGGAGAAGAA GAAAGACAAA GAGAAGAAGG AAAAGACAAA GGAAAAGGAG 
AAAGACAAGC CAAAATTAGA GAAAACTAGT CTAATGGCTG AAATTCCTCC TGCTGTCCCA 
CCGTCTCCTG TCATACCCCG ACTGACCCTC AGAGTGGGCG CCGGACAAGA CAAGATTGTG 
ATCAGTAAAG TGGTGCCAGA TACAGAGCCC ACTCCTCCTC CACCGCGGAC CCCAGTGGCC 
CGAATGGGTC CCGGAGCCCG AATACGTCCT CCTCCAGTTC TTCCTCCACC TGCCCCGCCT 
CCTGCAACCC CTCCACCTCG TCCTCCACCC GTGCTGCCTC CACCTCCACC TCCAGCGGCC 
CCCATCCAGA CTCCACCCAA CCAGGCTAAG GCTCGTGGCT GCAGCGTCGT CACCGAGACT 
GTCAGTGCTT ATGTGATTCG GGATGAATGG GGTAATCAGA TCTGGATTTG TCCAGGATGT 
AATAAAGCTG ATGACGGTAG TCCAATGATC GGATGTGATG AGTGCGATGA CTGGTATCAC 
TGGCCTTGTG TGGGTCTTCT TGCCGCTCCT CCCGAGGACC AGTCCTGGTT TTGCATAAAA 
TGTGCTGGCA AGAAGAAAGA CAAGAAGACA AAAAAAAGGA AACGCAAAGC GCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

The basal transcription complex component TAF3 transduces changes in nuclear phosphoinositides into transcriptional output.
Molecular cell58(3)453-67(2015 May)
Stijf-Bultsma Y,Sommer L,Tauber M,Baalbaki M,Giardoglou P,Jones DR,Gelato KA,van Pelt J,Shah Z,Rahnamoun H,Toma C,Anderson KE,Hawkins P,Lauberth SM,Haramis AP,Hart D,Fischle W,Divecha N

Quantitative mass spectrometry of TATA binding protein-containing complexes and subunit phosphorylations during the cell cycle.
Proteome science746(2009 Dec)
Pijnappel WP,Kolkman A,Baltissen MP,Heck AJ,Timmers HM

Selective interaction between Trf3 and Taf3 required for early development and hematopoiesis.
Developmental dynamics : an official publication of the American Association of Anatomists238(10)2540-9(2009 Oct)
Hart DO,Santra MK,Raha T,Green MR

The TFIID components human TAF(II)140 and Drosophila BIP2 (TAF(II)155) are novel metazoan homologues of yeast TAF(II)47 containing a histone fold and a PHD finger.
Molecular and cellular biology21(15)5109-21(2001 Aug)
Gangloff YG,Pointud JC,Thuault S,Carr? L,Romier C,Muratoglu S,Brand M,Tora L,Couderc JL,Davidson I

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taf3 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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