ptpn11a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ptpn11a
Entrez Gene ID	333979
Full Name	protein tyrosine phosphatase, non-receptor type 11, a
Synonyms	fa14b09,ptpn11,shp2,wu:fa14b09,wu:fi24f03,zgc:55388
General protein information	Preferred Names protein tyrosine phosphatase, non-receptor type 11, a Names tyrosine-protein phosphatase non-receptor type 11 protein tyrosine phosphatase SH-PTP2 protein tyrosine phosphatase, non-receptor type 11 (Noonan syndrome 1) protein-tyrosine phosphatase SHP-2a
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_199846.1	NP_956140.1	tyrosine-protein phosphatase non-receptor type 11

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	PTPN11	NP_990299.1
Danio rerio (zebrafish)	ptpn11a	NP_956140.1
	PTPN11	XP_001110117.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002438	XP_563746.4
Bos taurus (cattle)	PTPN11	XP_002694636.2
Rattus norvegicus (Norway rat)	Ptpn11	NP_001171064.1
Drosophila melanogaster (fruit fly)	csw	NP_001259174.1
Pan troglodytes (chimpanzee)	PTPN11	XP_522535.3
Canis lupus familiaris (dog)	PTPN11	XP_005636307.1
Homo sapiens (human)	PTPN11	NP_002825.3
Mus musculus (house mouse)	Ptpn11	NP_035332.1

Gene Ontology
Bibliography

Related articles in PubMed

The protein tyrosine phosphatase Shp2 regulates oligodendrocyte differentiation and early myelination and contributes to timely remyelination.
Ahrendsen JT, Harlow DE, Finseth LT, Bourne JN, Hickey SP, Gould EA, Culp CM, Macklin WB
The Journal of neuroscience : the official journal of the Society for Neuroscience(2017 Dec)

Shp2-MAPK signalling drives proliferation during zebrafish embryo caudal fin-fold regeneration.
Hale AJ, den Hertog J
Molecular and cellular biology(2017 Dec)

Differential oxidation of protein-tyrosine phosphatases during zebrafish caudal fin regeneration.
Wu W, Hale AJ, Lemeer S, den Hertog J
Scientific reports7(1)8460(2017 Aug)

Critical Role for GAB2 in Neuroblastoma Pathogenesis through the Promotion of SHP2/MYCN Cooperation.
Zhang X, Dong Z, Zhang C, Ung CY, He S, Tao T, Oliveira AM, Meves A, Ji B, Look AT, Li H, Neel BG, Zhu S
Cell reports18(12)2932-2942(2017 Mar)

Utilising polymorphisms to achieve allele-specific genome editing in zebrafish.
Capon SJ, Baillie GJ, Bower NI, da Silva JA, Paterson S, Hogan BM, Simons C, Smith KA
Biology open6(1)125-131(2017 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In MYCN transgenic fish, Gab2 overexpression activated the Shp2-Ras-Erk pathway, enhanced neuroblastoma induction, and increased tumor penetrance.
Title: Critical Role for GAB2 in Neuroblastoma Pathogenesis through the Promotion of SHP2/MYCN Cooperation.

Shp2 variant-mediated hyperactivation of MAPK signaling leads to impaired cilia function in Kupffer's vesicle, causing left-right asymmetry defects and defective early cardiac development.
Title: Noonan and LEOPARD syndrome Shp2 variants induce heart displacement defects in zebrafish.

Fer knockdown cooperated with NS and LS, but not wild type Shp2 to induce developmental defects, suggesting a role for Fer in the pathogenesis of both NS and LS.
Title: Phosphoproteomics-mediated identification of Fer kinase as a target of mutant Shp2 in Noonan and LEOPARD syndrome.

functions of ptpn11 genes in Zebrafish development
Title: Distinct and overlapping functions of ptpn11 genes in Zebrafish development.

Cell tracing experiments demonstrated that Shp2 knockdown induced defects in convergence and extension cell movements. In situ hybridization using a panel of markers indicated that cell fate was not affected by Shp2 knock down.
Title: Shp2 knockdown and Noonan/LEOPARD mutant Shp2-induced gastrulation defects.

The following ptpn11a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ptpn11a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa02011	NM_199846.1 Latest version!	Danio rerio protein tyrosine phosphatase non-receptor type 11a (ptpn11a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ptpn11a ( NM_199846.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956140.1 Danio rerio protein tyrosine phosphatase non-receptor type 11a (ptpn11a), mRNA.

Price & Availability↑

CloneID	ODa02011
Clone ID Related Accession (Same CDS sequence)	NM_199846.1
Accession Version	NM_199846.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1785bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-07
Organism	Danio rerio(zebrafish)
Product	tyrosine-protein phosphatase non-receptor type 11
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC045328.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC045328.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGACATCCC GAAGGTGGTT CCACCCCAAC ATCACTGGAG TGGAGGCAGA GAATCTCCTT 
CTCACTCGGG GTGTGGATGG GAGTTTTCTG GCCCGTCCCA GCAAAAGCAA CCCTGGAGAC 
TTTACACTTT CTGTCAGGCG AAATGGCGCT GTCACACACA TTAAGATTCA AAACACAGGA 
GACTATTACG ACCTGTATGG TGGAGAGAAG TTTGCCACTC TTGCTGAGCT GGTCCAGTAT 
TACATGGAGC ATCATGGACA GCTGAAAGAG AAAAACGGAG ACGTCATTGA GCTGAAATAC 
CCTCTGAACT GTGCCGATCC CACCTCTGAG AGGTGGTTTC ATGGTCACCT CTCTGGTCGT 
GAAGCTGAGA AGCTGTTGAC AGAGAAGGGC AAGAACGGAA GTTTCCTGGT GAGAGAAAGT 
CAGAGTCACC CAGGAGATTT TGTGCTCTCC GTACGAACTG GAGATGACAA GACGGACACC 
AGCGAAGGCA AACCCAAAGT CACACACGTC ATGATACGCT GCCAGCATGA CCTGAAGTAT 
GATGTTGGAG GTGGGGAGAA ATTTGACTCG CTAACGGATC TTGTGGAGCA CTACAAGAAA 
AATCCTATGG TGGAAACACT GGGGACAGTC CTGCAGCTTA AACAGCCTCT GAACACGACA 
CGCATCAATG CAGCAGAGAT CGAAAGTCGA GTACGAGAGC TCAGCAAGCT TGCAGAGGCC 
ACAGATAAAG TCAAGCAGGG ATTCTGGGAG GAGTTTGAGA CGCTTCAGCA ACAGGAATGT 
AAACTCCTCT ACAGTCGCAA AGAGGGTCAG CGACCAGAAA ACAAAAACAA GAACAGATAT 
AAGAACATTC TTCCCTTCGA CCACACTCGA GTCGTGCTCA CTGACGGCGA TGTGAATGAA 
CAAGGTTCTG ACTACATCAA CGCCAACCTC ATTATGCCTG ATAATGAGGC TAAAAGCAAC 
AATTCCAAGT TGAAGAGGAG CTACATTGCC ACACAAGGCT GCCTGCAGAA CACCATCAGC 
GATTTCTGGA GGATGGTGTT TCAGGAGAAC TCTCGAGTCA TCGTCATGAC CACAAAAGAA 
GTGGAACGGG GAAAGAGTAA GTGTGTGAAG TATTGGCCTG ATGTGTCTGC ACTGAAGGAG 
TACGGAGCCA TGCGTGTGCG CAATGTTAAG GAAACAATGG CGCACGATTA CATCCTGCGA 
GAGCTGAAGC TGTCTAAAGT TGGACAGGGA AATACAGAGC GTACAGTTTG GCAGTACCAT 
TTCCGTGCCT GGCCTGATCA CGGTGTGCCT GGAGACCCTG GTGGTGTCCT GGACTTCCTG 
GAAGAGGTCA AATTGAAGCA GGAGGGCATA ACTGGGGCCG GACCCATCGT GGTGCACTGC 
AGTGCTGGAA TTGGACGAAC AGGAACGTTT ATCGTGATTG ATATATTAAT AGACATCATC 
AGAGAAAAGG GGGTGGACTG CGATATCGAT GTGCCCAAGA CTATCCAGAT GGTGCGATCT 
CAGCGTTCAG GAATGGTACA GACTGAAGCA CAGTATCGCT TCATCTACAT GGCCGTACAG 
CATTATATTG AGACTCTGCA GCGCCGCATA GAGGAGGAGC AGAAAAGTAA AATTAAAGGA 
CGAGAGTACA CCAACATCAA ATATTCTCTC TCTGACCTGA GTGGAGGAGA TCAGAGCCCT 
CTGCCGCCCT GCACACCAAC ACCAACCTGT GCCGACATGA GGGACGACAG CTCTCGAGTC 
TATGAGAACG TGGGTCTCAT GCAGCAGCAG AAGAGTCACA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956140.1
CDS	195..1979
Translation	MTSRRWFHPNITGVEAENLLLTRGVDGSFLARPSKSNPGDFTLSVRRNGAVTHIKIQNTGDYYDLYGGEKFATLAELVQYYMEHHGQLKEKNGDVIELKYPLNCADPTSERWFHGHLSGREAEKLLTEKGKNGSFLVRESQSHPGDFVLSVRTGDDKTDTSEGKPKVTHVMIRCQHDLKYDVGGGEKFDSLTDLVEHYKKNPMVETLGTVLQLKQPLNTTRINAAEIESRVRELSKLAEATDKVKQGFWEEFETLQQQECKLLYSRKEGQRPENKNKNRYKNILPFDHTRVVLTDGDVNEQGSDYINANLIMPDNEAKSNNSKLKRSYIATQGCLQNTISDFWRMVFQENSRVIVMTTKEVERGKSKCVKYWPDVSALKEYGAMRVRNVKETMAHDYILRELKLSKVGQGNTERTVWQYHFRAWPDHGVPGDPGGVLDFLEEVKLKQEGITGAGPIVVHCSAGIGRTGTFIVIDILIDIIREKGVDCDIDVPKTIQMVRSQRSGMVQTEAQYRFIYMAVQHYIETLQRRIEEEQKSKIKGREYTNIKYSLSDLSGGDQSPLPPCTPTPTCADMRDDSSRVYENVGLMQQQKSHR

Target ORF information:

RefSeq Version	NM_199846.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase non-receptor type 11a (ptpn11a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199846.1

ORF Insert Sequence:

ATGACATCCC GAAGGTGGTT CCACCCCAAC ATCACTGGAG TGGAGGCAGA GAATCTCCTT 
CTCACTCGGG GTGTGGATGG GAGTTTTCTG GCCCGTCCCA GCAAAAGCAA CCCTGGAGAC 
TTTACACTTT CTGTCAGGCG AAATGGCGCT GTCACACACA TTAAGATTCA AAACACAGGA 
GACTATTACG ACCTGTATGG TGGAGAGAAG TTTGCCACTC TTGCTGAGCT GGTCCAGTAT 
TACATGGAGC ATCATGGACA GCTGAAAGAG AAAAACGGAG ACGTCATTGA GCTGAAATAC 
CCTCTGAACT GTGCCGATCC CACCTCTGAG AGGTGGTTTC ATGGTCACCT CTCTGGTCGT 
GAAGCTGAGA AGCTGTTGAC AGAGAAGGGC AAGAACGGAA GTTTCCTGGT GAGAGAAAGT 
CAGAGTCACC CAGGAGATTT TGTGCTCTCC GTACGAACTG GAGATGACAA GACGGACACC 
AGCGAAGGCA AACCCAAAGT CACACACGTC ATGATACGCT GCCAGCATGA CCTGAAGTAT 
GATGTTGGAG GTGGGGAGAA ATTTGACTCG CTAACGGATC TTGTGGAGCA CTACAAGAAA 
AATCCTATGG TGGAAACACT GGGGACAGTC CTGCAGCTTA AACAGCCTCT GAACACGACA 
CGCATCAATG CAGCAGAGAT CGAAAGTCGA GTACGAGAGC TCAGCAAGCT TGCAGAGGCC 
ACAGATAAAG TCAAGCAGGG ATTCTGGGAG GAGTTTGAGA CGCTTCAGCA ACAGGAATGT 
AAACTCCTCT ACAGTCGCAA AGAGGGTCAG CGACCAGAAA ACAAAAACAA GAACAGATAT 
AAGAACATTC TTCCCTTCGA CCACACTCGA GTCGTGCTCA CTGACGGCGA TGTGAATGAA 
CAAGGTTCTG ACTACATCAA CGCCAACCTC ATTATGCCTG ATAATGAGGC TAAAAGCAAC 
AATTCCAAGT TGAAGAGGAG CTACATTGCC ACACAAGGCT GCCTGCAGAA CACCATCAGC 
GATTTCTGGA GGATGGTGTT TCAGGAGAAC TCTCGAGTCA TCGTCATGAC CACAAAAGAA 
GTGGAACGGG GAAAGAGTAA GTGTGTGAAG TATTGGCCTG ATGTGTCTGC ACTGAAGGAG 
TACGGAGCCA TGCGTGTGCG CAATGTTAAG GAAACAATGG CGCACGATTA CATCCTGCGA 
GAGCTGAAGC TGTCTAAAGT TGGACAGGGA AATACAGAGC GTACAGTTTG GCAGTACCAT 
TTCCGTGCCT GGCCTGATCA CGGTGTGCCT GGAGACCCTG GTGGTGTCCT GGACTTCCTG 
GAAGAGGTCA AATTGAAGCA GGAGGGCATA ACTGGGGCCG GACCCATCGT GGTGCACTGC 
AGTGCTGGAA TTGGACGAAC AGGAACGTTT ATCGTGATTG ATATATTAAT AGACATCATC 
AGAGAAAAGG GGGTGGACTG CGATATCGAT GTGCCCAAGA CTATCCAGAT GGTGCGATCT 
CAGCGTTCAG GAATGGTACA GACTGAAGCA CAGTATCGCT TCATCTACAT GGCCGTACAG 
CATTATATTG AGACTCTGCA GCGCCGCATA GAGGAGGAGC AGAAAAGTAA AATTAAAGGA 
CGAGAGTACA CCAACATCAA ATATTCTCTC TCTGACCTGA GTGGAGGAGA TCAGAGCCCT 
CTGCCGCCCT GCACACCAAC ACCAACCTGT GCCGACATGA GGGACGACAG CTCTCGAGTC 
TATGAGAACG TGGGTCTCAT GCAGCAGCAG AAGAGTCACA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The protein tyrosine phosphatase Shp2 regulates oligodendrocyte differentiation and early myelination and contributes to timely remyelination.
The Journal of neuroscience : the official journal of the Society for Neuroscience(2017 Dec)
Ahrendsen JT,Harlow DE,Finseth LT,Bourne JN,Hickey SP,Gould EA,Culp CM,Macklin WB

Shp2-MAPK signalling drives proliferation during zebrafish embryo caudal fin-fold regeneration.
Molecular and cellular biology(2017 Dec)
Hale AJ,den Hertog J

Differential oxidation of protein-tyrosine phosphatases during zebrafish caudal fin regeneration.
Scientific reports7(1)8460(2017 Aug)
Wu W,Hale AJ,Lemeer S,den Hertog J

Critical Role for GAB2 in Neuroblastoma Pathogenesis through the Promotion of SHP2/MYCN Cooperation.
Cell reports18(12)2932-2942(2017 Mar)
Zhang X,Dong Z,Zhang C,Ung CY,He S,Tao T,Oliveira AM,Meves A,Ji B,Look AT,Li H,Neel BG,Zhu S

Utilising polymorphisms to achieve allele-specific genome editing in zebrafish.
Biology open6(1)125-131(2017 Jan)
Capon SJ,Baillie GJ,Bower NI,da Silva JA,Paterson S,Hogan BM,Simons C,Smith KA

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ptpn11a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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