rsf1b.1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	rsf1b.1
Entrez Gene ID	327618
Full Name	remodeling and spacing factor 1b, tandem duplicate 1
Synonyms	fi20e01,si:dkey-21n12.1,wu:fi20e01
General protein information	Preferred Names remodeling and spacing factor 1b, tandem duplicate 1 Names remodeling and spacing factor 1 uncharacterized protein LOC327618
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017358078.1	XP_017213567.1	remodeling and spacing factor 1 isoform X1
XM_017358080.1	XP_017213569.1	remodeling and spacing factor 1 isoform X3
XM_017358079.1	XP_017213568.1	remodeling and spacing factor 1 isoform X2
XM_017358078.2	XP_017213567.1	remodeling and spacing factor 1 isoform X1
XM_017358079.2	XP_017213568.1	remodeling and spacing factor 1 isoform X2
XM_017358080.2	XP_017213569.1	remodeling and spacing factor 1 isoform X3

Pathways from BioSystems

Homologies

	RSF1	XP_001091378.1
Canis lupus familiaris (dog)	RSF1	XP_534002.2
Rattus norvegicus (Norway rat)	Rsf1	XP_218939.4
Homo sapiens (human)	RSF1	NP_057662.3
Gallus gallus (chicken)	RSF1	XP_424124.3
Danio rerio (zebrafish)	si:dkey-21n12.1	XP_005169237.1
Bos taurus (cattle)	RSF1	XP_002699109.1
Mus musculus (house mouse)	Rsf1	NP_001074736.1
Pan troglodytes (chimpanzee)	RSF1	XP_001175061.2

Gene Ontology

The following rsf1b.1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rsf1b.1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa16390	XM_017358078.2 Latest version!	Danio rerio remodeling and spacing factor 1b, tandem duplicate 1 (rsf1b.1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa16391	XM_017358080.2 Latest version!	Danio rerio remodeling and spacing factor 1b, tandem duplicate 1 (rsf1b.1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa39952	XM_017358079.2 Latest version!	Danio rerio remodeling and spacing factor 1b, tandem duplicate 1 (rsf1b.1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
View Old Accession Versions >>
ODa16390	XM_017358078.1	Danio rerio remodeling and spacing factor 1b, tandem duplicate 1 (rsf1b.1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa39952	XM_017358079.1	Danio rerio remodeling and spacing factor 1b, tandem duplicate 1 (rsf1b.1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa16391	XM_017358080.1	Danio rerio remodeling and spacing factor 1b, tandem duplicate 1 (rsf1b.1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rsf1b.1 ( XM_017358078.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213567.1 Danio rerio remodeling and spacing factor 1b, tandem duplicate 1 (rsf1b.1), transcript variant X1, mRNA.

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CloneID	ODa16390
Clone ID Related Accession (Same CDS sequence)	XM_017358078.1 , XM_017358078.2
Accession Version	XM_017358078.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	remodeling and spacing factor 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCCT CGGAGGAAGC GGACGGTTCT TCCCCGGGAT TGTGCCCGAG CTTTGCCGTG 
ATCTGCTCGT TTCTGGAGCG GTACGGAGCT CTGCTGGACC TGCCGGAGGT GTCGTTCCCG 
GAGCTGGAGC GCAGCCTGCA GGACAGCAGC GCGGTACCCA AACTTCTTGT GGACCTTCAC 
GTGAAGTTAC TGAGGAAAAT TGGCAAGTCT GTGTCTGCAG AGAGATGGGA GAAATATCTC 
ATTAAGATTT GTCAGGAGTT TAACACCACA TGGGCATGGG AGTTGGAAAA GAAAGGTTAT 
CAAGAGTTGA CAGTGGAGTG TAAGACAGGA ATATTGAAGT ATTTGTGTGA ATGCCAGTTT 
GATGACAATG TCAAGTTTAA AAATGCTATC AATGAAGAAG ACCCGGATAA AATGCGACTT 
CAGCCTATTG GCCGAGACAA AGACGGCCTC ATGTACTGGT TCCAGCTGGA CCAAGACCAT 
AATGTAAGGA TCTATGTGGA AGAGCAGGAT GATTTGGATG GGTCGTCCTG GAAATGTATA 
GTAAGAAATA GAGATGACCT GGCTCAGATT CTTGGGTTGT TGAAGACTCA GATTGATCCA 
GCACTCCTGG TGAAGAAGGA ACAGGAGGAG GGCTCGTCTA ATAACAGTCC AAATCCCGAT 
GATGAGGAGA ACAAAAAGAA AGAGGGAGCT GAAGTGGAGA AGTCACAAGA TGATTTGTCC 
AAATCTGAAA ATAAGGAAAA CATATTAGAG AAAGAAAATA AAAAGGAAGA AGGTGCAGAT 
GCCACAGATG CTTTGTCGGA AAAGGGTTTA AAGGTAAAAG AGGAGCTGCA GAAACATTCA 
GAGACGGCAA ACGGATCTGA CGTTATTGCT GGATCAATCA AAGAAGAACC ACAGGATGAT 
GAGAAAGTCT TTTCCGCACC TTCAAAGAAA GCAGAACAGA CAGAGGAGGT GAAGAGAAAA 
ACACTAGAAG AGACCCAAAG AGCACTGAAA AATGACCATC AAGCTAAAAT CCCCCTGAAG 
AAGCGAGAGA TGAAGCTAAG TGAAGACTTT GACAGCAACA GCGCAGGACT TCGAAATGCT 
CCAGTAAAAG AGTCAAGGAC GTGTTTCGAT GATAGCATGT CGAGTGCTGA AAAGATCAAT 
GGCCATGTTC CTCACCTTCA GAAAGAAGCA ACAGATTCAG AAAGTCTCTC TCATTTTGAA 
TCCAATGAAA AAACAAAAGA CGCCCAGGTG GATAGAAAGA TGGACCCGAA TGAAACCAGA 
TTTCCTTCTG AAGAGAAGAT GGAAGTAGAG AAACCTAAGG AGAAAAATGA AGCTTCAACT 
AAACAGCACA AAACAACTCC TGGGGAAGAA AAACCAAGTG AACTATCAGA AAAGACACCT 
GATTCTGAAG TAGTAACTGA GAATATGGAA ACTATTGAAG ATATTAAGAC AACACCATGT 
GACGACACAG CAAATGAACT GCCTAAAGAG AAATCAATTA AAGATATTCC AAACAAAGAA 
TCAAAAGAAG ATTCATTGAA AGATGCACAA AGCAAAGAAT CAAAGGGAGA GTCATTGAAA 
GGAAGCAAAG AATCAAAAGG AGAATCATTA TTAAAAGATG CCCCAAGCAA AGAATCAAAA 
GAAGAATCAT TGAAAGATGC GCCGAGCAAA GAGTCAAAAG AATCATCATC AAAAGGTGCC 
CCAAGCAAAG AATCAAAAGG AGAATTATTT AAGGAAAGCA AAGAGTCGAA AAAAGAATCA 
TTAAAAGATA CCCCAAGTAA AGAATCAATA GGAGAATCAT TGAGAGATAC CCAAAGCAAA 
GAGTCAAAAG AATCATCATC AAAAGATGCC CCACGCAAAG AATCAAAAGG AGAATCATTG 
AAGGATGCCC CAAGCAAAGA GTCAAAAGAA GACTCAATGA AAGATACCCC AAGCAAAGAA 
TCAAATGAAT CATTATTGAA GGAAAGCAAA GAGTCAAAAG AAGAATCATT GAAAGATAAC 
CCAAGTAAAG AATCAAAAGA AGAAATATTG AAAGATACCC CAAGCAAAGA ATCAAAACAA 
GAATCATTGA AAGATACCCC AAGCAAAGAA TCAAAGGGAG AATCGTTGAA AGATGCCCCA 
AGCAAAGAAT CAAAAGAATC ATTATTGAAG GAAAGTAAAG AGTCAAAAGA AGAATCGTTG 
AAAGATCCCC CAGGCAAGGA GTCAGCAAAA TCAAAAGATG ACAAAACTAA AGCTCTTTCA 
AAAAAGGAAT CATTGGAAGA TATCTCAAAT GAAAAGTCAG CAAAATCTCT CAAAGATGAT 
GCAACAAAAG ATCCATCAAA AAAAGAATCA TTGCAAGATA TCTCAAACGA GTCAACAAAA 
TCTGTATCAA AAGACGACGC AGCACAAGAT CACTTTAACA AAGAATCATC AAAAGATCTC 
GCAAGCGAAG AGTCGGGGAA AGATCAGCCG GCAAAAGATC TTTCGAATGA AGAGTCTATA 
AAAAATCCTC CAAATCAAAA ACCAAGCAAA GATCTTGCAA AAAAAGAGTC ACAAAAGGTT 
ATCCCAGAAA GCGAATCACA AAAAGTTGTT TCAGAGAAAG ATTTGCAGAA GGGCATCCCA 
GAAAAAGGGT CACAGAAGAG TAACCCGGAA AAAGAGTCGC AGAAGAGTAA CCCGGAAAAA 
GAGTCGCAGA AGAGTAACCC GGAAAAAGAG TCGCAGAAGA GTAACCCGGA AAAAGAGTCG 
CAGAAGAGTA ACCCGGAAAA AGAGTCGCAG AAGAGTAACC CGGAAAAAGA GTCGCAGAAG 
AGTAACCCGG AAAAAGAGTC GCAGAATGTT CTCCCAAGTC AGAAATCTCC TAATAAAGAC 
CAAGAACAAG CAATGGAAAC CGAATCATTC CAGTCATCAA AATCAGTAGA GACCCGTGAA 
ATAATCAAAC CTCATTCTGA CACAGAAAAG CCAGAGAATG CTGAAAAAAC TAAGCTTCAT 
GAAAGCAACA CAGAAAAAGA GTCAACTCAA AAACCATGCA TCTCTGAAGG AGACAAACTC 
TTAACCAGGG GAGAGTCTAT TGGTTCTAAA CCCTGTGAAG ATACGGATAA CCCTCAAGAC 
AAAAAACAAG AGAAGTCTGG CTCCTCCAAA TCGAGAACAG ATACTAATAA ATCATCTGTT 
TCTGATTCAA AAAAGTCAGA AACCAGAAAG AAAGTAGATG CTGAGGACAA ATGTGATGCA 
GCTGAAACGG CTAAAAGTGA CAAATCAGAA ATAGCAGAAG ACAAAGATAA AAAGAGTGAA 
AGTACAAAAA CATCAGATGA AGTTAAAGCT CCAGAAAAGC CCAATAAATC AGATAAAGAA 
CATGAAAAGC AACCAAATCC AATGGAAACT GATTCAGAGA TCCCTAAAAC TGCTGAGGAG 
ACAACAAAAG AAAGCGTGAA GGAAGCCAAT GCTACTGTAG ATAACTGCAC CAGCAAACAA 
TCAGAGCGAG ACGCGACTTC TGAAGAACGC GAGGCTTCCG GTGTTGTTCA AGAAAAGGGG 
AAAGGCATTA AAAAACCAGG ACGGCCTTCA AAGAAATCTA CTGAGAGTCA AAATAAGGAT 
AAAACTGTAG AAGAGGAAAA AGATGATGGA AAGCGACGGG GAAGAACCCC TCTGAAGTTG 
AAGAAACCAG CTCACAGGAG AAAGGCAGAG ATTCAGAGAG AAGAGCTGAA GGGAGATTCG 
GAGTCTGAGA CGACTGATGG ACGGTGTTTG AGAAGGTCTC CCAGAATCTG CAGACCAACC 
GCTAAAGCAG TGGAGATCCA GGATAAGAGG GCGGAGCGGA AACAGGCCAC GCCAGAGAAG 
GAGAAGGAGG AAGAAGAAGA AGAAGAGGAG GAGGAGCCTG TACAGAGGAA ACCGAGGAAA 
GCAGAGCCAG ATGGACAGAC AAAACCAAAG CAGGTGGGGA GACGTCGAAA ACGACAACCG 
TGGTCTCGCA TGAGGCGTAA AAAGAAATCT TCGGAGGATG AGGAAGATGA AGAGGAGAGC 
GATAGCGAAG AAGAGGAGAC AGAGGAGGAT GACAGCGATG AAGACTACAA GGTGGAGAAA 
AGCAAGAAGA GAAGGAGAAA CCGAAACCGC GAACGGCGCA GCTCTGACTC CTCCACCTCT 
TCATCAGAAG ACGATACACC CAATGACGAC CCCTGCAAAC ACTGTGGCCT TCCCAATCAT 
CCCGAACTGA TCCTGCTGTG TGATTCCTGT GACAGCGGCT ACCACACCGC CTGCCTGCGT 
CCTCCGCTCA TGATCATTCC AGACGGAGAG TGGTTCTGTC CTCCCTGCCA GCACAAGCTA 
CTTTGCGACA GACTGGAAGA GCAGCTGCTG AATTTGGATG CCGCCCTCAA AAAGAAAGAA 
CGAGCAGAGC GAAGGAAAGA GCGACTGGTG TATGTTGGCA TTAGTGTGGA GAACATCATC 
ACGCCCTCTG TTGAAGTGGA GGAAGAAAAG GAAGAGGTTT TGGTTAAGGA TAAAGCGGAG 
ACGAAGAAGA GCAAAGGCTG GGGACGGAGA TCAACCAGAG CCAAGAAATA CATAAGCTAC 
AGATTCGATG AGTTTGATGA AGCGATCGAA GAGGCGATTG AGGAGGACAT TAAAGAGGCA 
GAAGGTGGAG GAGCAGGACG AGGGAAGGAC ATGGCGAACA TCACAGGCCA CAGAGGGAAG 
GACATGTCCA CCATCCTGCA GGAGGAAGGC AAAGAGAACG GCCGACCACA AAGAACTAGC 
GCAGCGCAGA AGAAGAAACG GCGGCGTCTC AATGATCTGG ACAGCGACAG CACTGTAGAT 
GAGGAGGAGA GCGAAGATGA GTTTCGCCTC AGCGACAGCA GTTCTGAGGA GGAGTTTGTG 
GCGTCTGAAA ACGCTGAAAG TGAAGACAAT GCGCCATCCT TAGAGGACAG CGACTTTGAC 
AGTGATGCTC AAGGTTCACG AGTGAATCGT GCGAACAGAA GGAAAACGAG CCGGAGTCGA 
ATCCGGAGAA AACAACCTCC GCGCAGGAGA CGCAGACCCA GAGGATACTC TGATGAAGAG 
GAGCTGGAGA CTGATGAAGA GGAGGAGGAA GAGGACGAGA TGGTGACGGA GGGCTCTAGT 
GAGTTCAGTG ACAGTGATCT GGACGTCCGC TGTCGACGCT CGTATCGCAG CCGAAAGCAG 
GAGGTGAATT ACTGTGAAAC TTCAGATTCA GACGGCTCGC AAGCCAGCAC CAACAAAGAC 
AAGAAAAAGA AGCGCAGACG ACTGTCCAGC TCTGACAGCG AAGCCAGTTT CCAGTCAGCG 
GAATCGGAAG AAGAAGAGCG AAAACCCAAG AAGATCAGAG CCGACTCGTC TGAGGAGGAG 
GAATCCAGGA AGCGACGCAG ACGTTTATCC CTAAAGCGCC GACGGGAGTC AGAGGACGAT 
GACGATGATG ATGATTCAGA TGAATCCGAG GAAGAGGAGC GTCCTGTCCG GAAGCGAGTA 
AACCGAATCG ACTCTGACGA AAGTGAGAGC GAAGAAGAGG AGAAGGAGGT GAAGAAGAGT 
CCGCAGGAGA AAGACACAGA AGAGACGAGC GCTAGTAAAG GTCCCAGTCC AATAAACGAC 
AACACAGCGG AGCCTCCTTC TACTAATGGA CAGAGCCCTA TAAAGGGTCT GGAGGGCGTC 
GCGCCACGGC CGGGAGCAGG AGCAACGCCT AAAAATGGCA ACACACCATC CATAGCACCC 
AATGGCCTGG AAGCCGGCGC ACAGGAAGAA GACGAAGACG ATCTTTTGGG CGTCACAGAC 
CTCGTAGACT ATGTCTGCAA CAGCGAACAG TTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213567.1
CDS	52..5787
Translation	MSASEEADGSSPGLCPSFAVICSFLERYGALLDLPEVSFPELERSLQDSSAVPKLLVDLHVKLLRKIGKSVSAERWEKYLIKICQEFNTTWAWELEKKGYQELTVECKTGILKYLCECQFDDNVKFKNAINEEDPDKMRLQPIGRDKDGLMYWFQLDQDHNVRIYVEEQDDLDGSSWKCIVRNRDDLAQILGLLKTQIDPALLVKKEQEEGSSNNSPNPDDEENKKKEGAEVEKSQDDLSKSENKENILEKENKKEEGADATDALSEKGLKVKEELQKHSETANGSDVIAGSIKEEPQDDEKVFSAPSKKAEQTEEVKRKTLEETQRALKNDHQAKIPLKKREMKLSEDFDSNSAGLRNAPVKESRTCFDDSMSSAEKINGHVPHLQKEATDSESLSHFESNEKTKDAQVDRKMDPNETRFPSEEKMEVEKPKEKNEASTKQHKTTPGEEKPSELSEKTPDSEVVTENMETIEDIKTTPCDDTANELPKEKSIKDIPNKESKEDSLKDAQSKESKGESLKGSKESKGESLLKDAPSKESKEESLKDAPSKESKESSSKGAPSKESKGELFKESKESKKESLKDTPSKESIGESLRDTQSKESKESSSKDAPRKESKGESLKDAPSKESKEDSMKDTPSKESNESLLKESKESKEESLKDNPSKESKEEILKDTPSKESKQESLKDTPSKESKGESLKDAPSKESKESLLKESKESKEESLKDPPGKESAKSKDDKTKALSKKESLEDISNEKSAKSLKDDATKDPSKKESLQDISNESTKSVSKDDAAQDHFNKESSKDLASEESGKDQPAKDLSNEESIKNPPNQKPSKDLAKKESQKVIPESESQKVVSEKDLQKGIPEKGSQKSNPEKESQKSNPEKESQKSNPEKESQKSNPEKESQKSNPEKESQKSNPEKESQKSNPEKESQNVLPSQKSPNKDQEQAMETESFQSSKSVETREIIKPHSDTEKPENAEKTKLHESNTEKESTQKPCISEGDKLLTRGESIGSKPCEDTDNPQDKKQEKSGSSKSRTDTNKSSVSDSKKSETRKKVDAEDKCDAAETAKSDKSEIAEDKDKKSESTKTSDEVKAPEKPNKSDKEHEKQPNPMETDSEIPKTAEETTKESVKEANATVDNCTSKQSERDATSEEREASGVVQEKGKGIKKPGRPSKKSTESQNKDKTVEEEKDDGKRRGRTPLKLKKPAHRRKAEIQREELKGDSESETTDGRCLRRSPRICRPTAKAVEIQDKRAERKQATPEKEKEEEEEEEEEEPVQRKPRKAEPDGQTKPKQVGRRRKRQPWSRMRRKKKSSEDEEDEEESDSEEEETEEDDSDEDYKVEKSKKRRRNRNRERRSSDSSTSSSEDDTPNDDPCKHCGLPNHPELILLCDSCDSGYHTACLRPPLMIIPDGEWFCPPCQHKLLCDRLEEQLLNLDAALKKKERAERRKERLVYVGISVENIITPSVEVEEEKEEVLVKDKAETKKSKGWGRRSTRAKKYISYRFDEFDEAIEEAIEEDIKEAEGGGAGRGKDMANITGHRGKDMSTILQEEGKENGRPQRTSAAQKKKRRRLNDLDSDSTVDEEESEDEFRLSDSSSEEEFVASENAESEDNAPSLEDSDFDSDAQGSRVNRANRRKTSRSRIRRKQPPRRRRRPRGYSDEEELETDEEEEEEDEMVTEGSSEFSDSDLDVRCRRSYRSRKQEVNYCETSDSDGSQASTNKDKKKKRRRLSSSDSEASFQSAESEEEERKPKKIRADSSEEEESRKRRRRLSLKRRRESEDDDDDDDSDESEEEERPVRKRVNRIDSDESESEEEEKEVKKSPQEKDTEETSASKGPSPINDNTAEPPSTNGQSPIKGLEGVAPRPGAGATPKNGNTPSIAPNGLEAGAQEEDEDDLLGVTDLVDYVCNSEQL

Target ORF information:

RefSeq Version	XM_017358078.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio remodeling and spacing factor 1b, tandem duplicate 1 (rsf1b.1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358078.1

ORF Insert Sequence:

ATGTCTGCCT CGGAGGAAGC GGACGGTTCT TCCCCGGGAT TGTGCCCGAG CTTTGCCGTG 
ATCTGCTCGT TTCTGGAGCG GTACGGAGCT CTGCTGGACC TGCCGGAGGT GTCGTTCCCG 
GAGCTGGAGC GCAGCCTGCA GGACAGCAGC GCGGTACCCA AACTTCTTGT GGACCTTCAC 
GTGAAGTTAC TGAGGAAAAT TGGCAAGTCT GTGTCTGCAG AGAGATGGGA GAAATATCTC 
ATTAAGATTT GTCAGGAGTT TAACACCACA TGGGCATGGG AGTTGGAAAA GAAAGGTTAT 
CAAGAGTTGA CAGTGGAGTG TAAGACAGGA ATATTGAAGT ATTTGTGTGA ATGCCAGTTT 
GATGACAATG TCAAGTTTAA AAATGCTATC AATGAAGAAG ACCCGGATAA AATGCGACTT 
CAGCCTATTG GCCGAGACAA AGACGGCCTC ATGTACTGGT TCCAGCTGGA CCAAGACCAT 
AATGTAAGGA TCTATGTGGA AGAGCAGGAT GATTTGGATG GGTCGTCCTG GAAATGTATA 
GTAAGAAATA GAGATGACCT GGCTCAGATT CTTGGGTTGT TGAAGACTCA GATTGATCCA 
GCACTCCTGG TGAAGAAGGA ACAGGAGGAG GGCTCGTCTA ATAACAGTCC AAATCCCGAT 
GATGAGGAGA ACAAAAAGAA AGAGGGAGCT GAAGTGGAGA AGTCACAAGA TGATTTGTCC 
AAATCTGAAA ATAAGGAAAA CATATTAGAG AAAGAAAATA AAAAGGAAGA AGGTGCAGAT 
GCCACAGATG CTTTGTCGGA AAAGGGTTTA AAGGTAAAAG AGGAGCTGCA GAAACATTCA 
GAGACGGCAA ACGGATCTGA CGTTATTGCT GGATCAATCA AAGAAGAACC ACAGGATGAT 
GAGAAAGTCT TTTCCGCACC TTCAAAGAAA GCAGAACAGA CAGAGGAGGT GAAGAGAAAA 
ACACTAGAAG AGACCCAAAG AGCACTGAAA AATGACCATC AAGCTAAAAT CCCCCTGAAG 
AAGCGAGAGA TGAAGCTAAG TGAAGACTTT GACAGCAACA GCGCAGGACT TCGAAATGCT 
CCAGTAAAAG AGTCAAGGAC GTGTTTCGAT GATAGCATGT CGAGTGCTGA AAAGATCAAT 
GGCCATGTTC CTCACCTTCA GAAAGAAGCA ACAGATTCAG AAAGTCTCTC TCATTTTGAA 
TCCAATGAAA AAACAAAAGA CGCCCAGGTG GATAGAAAGA TGGACCCGAA TGAAACCAGA 
TTTCCTTCTG AAGAGAAGAT GGAAGTAGAG AAACCTAAGG AGAAAAATGA AGCTTCAACT 
AAACAGCACA AAACAACTCC TGGGGAAGAA AAACCAAGTG AACTATCAGA AAAGACACCT 
GATTCTGAAG TAGTAACTGA GAATATGGAA ACTATTGAAG ATATTAAGAC AACACCATGT 
GACGACACAG CAAATGAACT GCCTAAAGAG AAATCAATTA AAGATATTCC AAACAAAGAA 
TCAAAAGAAG ATTCATTGAA AGATGCACAA AGCAAAGAAT CAAAGGGAGA GTCATTGAAA 
GGAAGCAAAG AATCAAAAGG AGAATCATTA TTAAAAGATG CCCCAAGCAA AGAATCAAAA 
GAAGAATCAT TGAAAGATGC GCCGAGCAAA GAGTCAAAAG AATCATCATC AAAAGGTGCC 
CCAAGCAAAG AATCAAAAGG AGAATTATTT AAGGAAAGCA AAGAGTCGAA AAAAGAATCA 
TTAAAAGATA CCCCAAGTAA AGAATCAATA GGAGAATCAT TGAGAGATAC CCAAAGCAAA 
GAGTCAAAAG AATCATCATC AAAAGATGCC CCACGCAAAG AATCAAAAGG AGAATCATTG 
AAGGATGCCC CAAGCAAAGA GTCAAAAGAA GACTCAATGA AAGATACCCC AAGCAAAGAA 
TCAAATGAAT CATTATTGAA GGAAAGCAAA GAGTCAAAAG AAGAATCATT GAAAGATAAC 
CCAAGTAAAG AATCAAAAGA AGAAATATTG AAAGATACCC CAAGCAAAGA ATCAAAACAA 
GAATCATTGA AAGATACCCC AAGCAAAGAA TCAAAGGGAG AATCGTTGAA AGATGCCCCA 
AGCAAAGAAT CAAAAGAATC ATTATTGAAG GAAAGTAAAG AGTCAAAAGA AGAATCGTTG 
AAAGATCCCC CAGGCAAGGA GTCAGCAAAA TCAAAAGATG ACAAAACTAA AGCTCTTTCA 
AAAAAGGAAT CATTGGAAGA TATCTCAAAT GAAAAGTCAG CAAAATCTCT CAAAGATGAT 
GCAACAAAAG ATCCATCAAA AAAAGAATCA TTGCAAGATA TCTCAAACGA GTCAACAAAA 
TCTGTATCAA AAGACGACGC AGCACAAGAT CACTTTAACA AAGAATCATC AAAAGATCTC 
GCAAGCGAAG AGTCGGGGAA AGATCAGCCG GCAAAAGATC TTTCGAATGA AGAGTCTATA 
AAAAATCCTC CAAATCAAAA ACCAAGCAAA GATCTTGCAA AAAAAGAGTC ACAAAAGGTT 
ATCCCAGAAA GCGAATCACA AAAAGTTGTT TCAGAGAAAG ATTTGCAGAA GGGCATCCCA 
GAAAAAGGGT CACAGAAGAG TAACCCGGAA AAAGAGTCGC AGAAGAGTAA CCCGGAAAAA 
GAGTCGCAGA AGAGTAACCC GGAAAAAGAG TCGCAGAAGA GTAACCCGGA AAAAGAGTCG 
CAGAAGAGTA ACCCGGAAAA AGAGTCGCAG AAGAGTAACC CGGAAAAAGA GTCGCAGAAG 
AGTAACCCGG AAAAAGAGTC GCAGAATGTT CTCCCAAGTC AGAAATCTCC TAATAAAGAC 
CAAGAACAAG CAATGGAAAC CGAATCATTC CAGTCATCAA AATCAGTAGA GACCCGTGAA 
ATAATCAAAC CTCATTCTGA CACAGAAAAG CCAGAGAATG CTGAAAAAAC TAAGCTTCAT 
GAAAGCAACA CAGAAAAAGA GTCAACTCAA AAACCATGCA TCTCTGAAGG AGACAAACTC 
TTAACCAGGG GAGAGTCTAT TGGTTCTAAA CCCTGTGAAG ATACGGATAA CCCTCAAGAC 
AAAAAACAAG AGAAGTCTGG CTCCTCCAAA TCGAGAACAG ATACTAATAA ATCATCTGTT 
TCTGATTCAA AAAAGTCAGA AACCAGAAAG AAAGTAGATG CTGAGGACAA ATGTGATGCA 
GCTGAAACGG CTAAAAGTGA CAAATCAGAA ATAGCAGAAG ACAAAGATAA AAAGAGTGAA 
AGTACAAAAA CATCAGATGA AGTTAAAGCT CCAGAAAAGC CCAATAAATC AGATAAAGAA 
CATGAAAAGC AACCAAATCC AATGGAAACT GATTCAGAGA TCCCTAAAAC TGCTGAGGAG 
ACAACAAAAG AAAGCGTGAA GGAAGCCAAT GCTACTGTAG ATAACTGCAC CAGCAAACAA 
TCAGAGCGAG ACGCGACTTC TGAAGAACGC GAGGCTTCCG GTGTTGTTCA AGAAAAGGGG 
AAAGGCATTA AAAAACCAGG ACGGCCTTCA AAGAAATCTA CTGAGAGTCA AAATAAGGAT 
AAAACTGTAG AAGAGGAAAA AGATGATGGA AAGCGACGGG GAAGAACCCC TCTGAAGTTG 
AAGAAACCAG CTCACAGGAG AAAGGCAGAG ATTCAGAGAG AAGAGCTGAA GGGAGATTCG 
GAGTCTGAGA CGACTGATGG ACGGTGTTTG AGAAGGTCTC CCAGAATCTG CAGACCAACC 
GCTAAAGCAG TGGAGATCCA GGATAAGAGG GCGGAGCGGA AACAGGCCAC GCCAGAGAAG 
GAGAAGGAGG AAGAAGAAGA AGAAGAGGAG GAGGAGCCTG TACAGAGGAA ACCGAGGAAA 
GCAGAGCCAG ATGGACAGAC AAAACCAAAG CAGGTGGGGA GACGTCGAAA ACGACAACCG 
TGGTCTCGCA TGAGGCGTAA AAAGAAATCT TCGGAGGATG AGGAAGATGA AGAGGAGAGC 
GATAGCGAAG AAGAGGAGAC AGAGGAGGAT GACAGCGATG AAGACTACAA GGTGGAGAAA 
AGCAAGAAGA GAAGGAGAAA CCGAAACCGC GAACGGCGCA GCTCTGACTC CTCCACCTCT 
TCATCAGAAG ACGATACACC CAATGACGAC CCCTGCAAAC ACTGTGGCCT TCCCAATCAT 
CCCGAACTGA TCCTGCTGTG TGATTCCTGT GACAGCGGCT ACCACACCGC CTGCCTGCGT 
CCTCCGCTCA TGATCATTCC AGACGGAGAG TGGTTCTGTC CTCCCTGCCA GCACAAGCTA 
CTTTGCGACA GACTGGAAGA GCAGCTGCTG AATTTGGATG CCGCCCTCAA AAAGAAAGAA 
CGAGCAGAGC GAAGGAAAGA GCGACTGGTG TATGTTGGCA TTAGTGTGGA GAACATCATC 
ACGCCCTCTG TTGAAGTGGA GGAAGAAAAG GAAGAGGTTT TGGTTAAGGA TAAAGCGGAG 
ACGAAGAAGA GCAAAGGCTG GGGACGGAGA TCAACCAGAG CCAAGAAATA CATAAGCTAC 
AGATTCGATG AGTTTGATGA AGCGATCGAA GAGGCGATTG AGGAGGACAT TAAAGAGGCA 
GAAGGTGGAG GAGCAGGACG AGGGAAGGAC ATGGCGAACA TCACAGGCCA CAGAGGGAAG 
GACATGTCCA CCATCCTGCA GGAGGAAGGC AAAGAGAACG GCCGACCACA AAGAACTAGC 
GCAGCGCAGA AGAAGAAACG GCGGCGTCTC AATGATCTGG ACAGCGACAG CACTGTAGAT 
GAGGAGGAGA GCGAAGATGA GTTTCGCCTC AGCGACAGCA GTTCTGAGGA GGAGTTTGTG 
GCGTCTGAAA ACGCTGAAAG TGAAGACAAT GCGCCATCCT TAGAGGACAG CGACTTTGAC 
AGTGATGCTC AAGGTTCACG AGTGAATCGT GCGAACAGAA GGAAAACGAG CCGGAGTCGA 
ATCCGGAGAA AACAACCTCC GCGCAGGAGA CGCAGACCCA GAGGATACTC TGATGAAGAG 
GAGCTGGAGA CTGATGAAGA GGAGGAGGAA GAGGACGAGA TGGTGACGGA GGGCTCTAGT 
GAGTTCAGTG ACAGTGATCT GGACGTCCGC TGTCGACGCT CGTATCGCAG CCGAAAGCAG 
GAGGTGAATT ACTGTGAAAC TTCAGATTCA GACGGCTCGC AAGCCAGCAC CAACAAAGAC 
AAGAAAAAGA AGCGCAGACG ACTGTCCAGC TCTGACAGCG AAGCCAGTTT CCAGTCAGCG 
GAATCGGAAG AAGAAGAGCG AAAACCCAAG AAGATCAGAG CCGACTCGTC TGAGGAGGAG 
GAATCCAGGA AGCGACGCAG ACGTTTATCC CTAAAGCGCC GACGGGAGTC AGAGGACGAT 
GACGATGATG ATGATTCAGA TGAATCCGAG GAAGAGGAGC GTCCTGTCCG GAAGCGAGTA 
AACCGAATCG ACTCTGACGA AAGTGAGAGC GAAGAAGAGG AGAAGGAGGT GAAGAAGAGT 
CCGCAGGAGA AAGACACAGA AGAGACGAGC GCTAGTAAAG GTCCCAGTCC AATAAACGAC 
AACACAGCGG AGCCTCCTTC TACTAATGGA CAGAGCCCTA TAAAGGGTCT GGAGGGCGTC 
GCGCCACGGC CGGGAGCAGG AGCAACGCCT AAAAATGGCA ACACACCATC CATAGCACCC 
AATGGCCTGG AAGCCGGCGC ACAGGAAGAA GACGAAGACG ATCTTTTGGG CGTCACAGAC 
CTCGTAGACT ATGTCTGCAA CAGCGAACAG TTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rsf1b.1 ( XM_017358078.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213567.1 Danio rerio remodeling and spacing factor 1b, tandem duplicate 1 (rsf1b.1), transcript variant X1, mRNA.

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CloneID	ODa16390
Clone ID Related Accession (Same CDS sequence)	XM_017358078.1 , XM_017358078.2
Accession Version	XM_017358078.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	remodeling and spacing factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017358078.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCCT CGGAGGAAGC GGACGGTTCT TCCCCGGGAT TGTGCCCGAG CTTTGCCGTG 
ATCTGCTCGT TTCTGGAGCG GTACGGAGCT CTGCTGGACC TGCCGGAGGT GTCGTTCCCG 
GAGCTGGAGC GCAGCCTGCA GGACAGCAGC GCGGTACCCA AACTTCTTGT GGACCTTCAC 
GTGAAGTTAC TGAGGAAAAT TGGCAAGTCT GTGTCTGCAG AGAGATGGGA GAAATATCTC 
ATTAAGATTT GTCAGGAGTT TAACACCACA TGGGCATGGG AGTTGGAAAA GAAAGGTTAT 
CAAGAGTTGA CAGTGGAGTG TAAGACAGGA ATATTGAAGT ATTTGTGTGA ATGCCAGTTT 
GATGACAATG TCAAGTTTAA AAATGCTATC AATGAAGAAG ACCCGGATAA AATGCGACTT 
CAGCCTATTG GCCGAGACAA AGACGGCCTC ATGTACTGGT TCCAGCTGGA CCAAGACCAT 
AATGTAAGGA TCTATGTGGA AGAGCAGGAT GATTTGGATG GGTCGTCCTG GAAATGTATA 
GTAAGAAATA GAGATGACCT GGCTCAGATT CTTGGGTTGT TGAAGACTCA GATTGATCCA 
GCACTCCTGG TGAAGAAGGA ACAGGAGGAG GGCTCGTCTA ATAACAGTCC AAATCCCGAT 
GATGAGGAGA ACAAAAAGAA AGAGGGAGCT GAAGTGGAGA AGTCACAAGA TGATTTGTCC 
AAATCTGAAA ATAAGGAAAA CATATTAGAG AAAGAAAATA AAAAGGAAGA AGGTGCAGAT 
GCCACAGATG CTTTGTCGGA AAAGGGTTTA AAGGTAAAAG AGGAGCTGCA GAAACATTCA 
GAGACGGCAA ACGGATCTGA CGTTATTGCT GGATCAATCA AAGAAGAACC ACAGGATGAT 
GAGAAAGTCT TTTCCGCACC TTCAAAGAAA GCAGAACAGA CAGAGGAGGT GAAGAGAAAA 
ACACTAGAAG AGACCCAAAG AGCACTGAAA AATGACCATC AAGCTAAAAT CCCCCTGAAG 
AAGCGAGAGA TGAAGCTAAG TGAAGACTTT GACAGCAACA GCGCAGGACT TCGAAATGCT 
CCAGTAAAAG AGTCAAGGAC GTGTTTCGAT GATAGCATGT CGAGTGCTGA AAAGATCAAT 
GGCCATGTTC CTCACCTTCA GAAAGAAGCA ACAGATTCAG AAAGTCTCTC TCATTTTGAA 
TCCAATGAAA AAACAAAAGA CGCCCAGGTG GATAGAAAGA TGGACCCGAA TGAAACCAGA 
TTTCCTTCTG AAGAGAAGAT GGAAGTAGAG AAACCTAAGG AGAAAAATGA AGCTTCAACT 
AAACAGCACA AAACAACTCC TGGGGAAGAA AAACCAAGTG AACTATCAGA AAAGACACCT 
GATTCTGAAG TAGTAACTGA GAATATGGAA ACTATTGAAG ATATTAAGAC AACACCATGT 
GACGACACAG CAAATGAACT GCCTAAAGAG AAATCAATTA AAGATATTCC AAACAAAGAA 
TCAAAAGAAG ATTCATTGAA AGATGCACAA AGCAAAGAAT CAAAGGGAGA GTCATTGAAA 
GGAAGCAAAG AATCAAAAGG AGAATCATTA TTAAAAGATG CCCCAAGCAA AGAATCAAAA 
GAAGAATCAT TGAAAGATGC GCCGAGCAAA GAGTCAAAAG AATCATCATC AAAAGGTGCC 
CCAAGCAAAG AATCAAAAGG AGAATTATTT AAGGAAAGCA AAGAGTCGAA AAAAGAATCA 
TTAAAAGATA CCCCAAGTAA AGAATCAATA GGAGAATCAT TGAGAGATAC CCAAAGCAAA 
GAGTCAAAAG AATCATCATC AAAAGATGCC CCACGCAAAG AATCAAAAGG AGAATCATTG 
AAGGATGCCC CAAGCAAAGA GTCAAAAGAA GACTCAATGA AAGATACCCC AAGCAAAGAA 
TCAAATGAAT CATTATTGAA GGAAAGCAAA GAGTCAAAAG AAGAATCATT GAAAGATAAC 
CCAAGTAAAG AATCAAAAGA AGAAATATTG AAAGATACCC CAAGCAAAGA ATCAAAACAA 
GAATCATTGA AAGATACCCC AAGCAAAGAA TCAAAGGGAG AATCGTTGAA AGATGCCCCA 
AGCAAAGAAT CAAAAGAATC ATTATTGAAG GAAAGTAAAG AGTCAAAAGA AGAATCGTTG 
AAAGATCCCC CAGGCAAGGA GTCAGCAAAA TCAAAAGATG ACAAAACTAA AGCTCTTTCA 
AAAAAGGAAT CATTGGAAGA TATCTCAAAT GAAAAGTCAG CAAAATCTCT CAAAGATGAT 
GCAACAAAAG ATCCATCAAA AAAAGAATCA TTGCAAGATA TCTCAAACGA GTCAACAAAA 
TCTGTATCAA AAGACGACGC AGCACAAGAT CACTTTAACA AAGAATCATC AAAAGATCTC 
GCAAGCGAAG AGTCGGGGAA AGATCAGCCG GCAAAAGATC TTTCGAATGA AGAGTCTATA 
AAAAATCCTC CAAATCAAAA ACCAAGCAAA GATCTTGCAA AAAAAGAGTC ACAAAAGGTT 
ATCCCAGAAA GCGAATCACA AAAAGTTGTT TCAGAGAAAG ATTTGCAGAA GGGCATCCCA 
GAAAAAGGGT CACAGAAGAG TAACCCGGAA AAAGAGTCGC AGAAGAGTAA CCCGGAAAAA 
GAGTCGCAGA AGAGTAACCC GGAAAAAGAG TCGCAGAAGA GTAACCCGGA AAAAGAGTCG 
CAGAAGAGTA ACCCGGAAAA AGAGTCGCAG AAGAGTAACC CGGAAAAAGA GTCGCAGAAG 
AGTAACCCGG AAAAAGAGTC GCAGAATGTT CTCCCAAGTC AGAAATCTCC TAATAAAGAC 
CAAGAACAAG CAATGGAAAC CGAATCATTC CAGTCATCAA AATCAGTAGA GACCCGTGAA 
ATAATCAAAC CTCATTCTGA CACAGAAAAG CCAGAGAATG CTGAAAAAAC TAAGCTTCAT 
GAAAGCAACA CAGAAAAAGA GTCAACTCAA AAACCATGCA TCTCTGAAGG AGACAAACTC 
TTAACCAGGG GAGAGTCTAT TGGTTCTAAA CCCTGTGAAG ATACGGATAA CCCTCAAGAC 
AAAAAACAAG AGAAGTCTGG CTCCTCCAAA TCGAGAACAG ATACTAATAA ATCATCTGTT 
TCTGATTCAA AAAAGTCAGA AACCAGAAAG AAAGTAGATG CTGAGGACAA ATGTGATGCA 
GCTGAAACGG CTAAAAGTGA CAAATCAGAA ATAGCAGAAG ACAAAGATAA AAAGAGTGAA 
AGTACAAAAA CATCAGATGA AGTTAAAGCT CCAGAAAAGC CCAATAAATC AGATAAAGAA 
CATGAAAAGC AACCAAATCC AATGGAAACT GATTCAGAGA TCCCTAAAAC TGCTGAGGAG 
ACAACAAAAG AAAGCGTGAA GGAAGCCAAT GCTACTGTAG ATAACTGCAC CAGCAAACAA 
TCAGAGCGAG ACGCGACTTC TGAAGAACGC GAGGCTTCCG GTGTTGTTCA AGAAAAGGGG 
AAAGGCATTA AAAAACCAGG ACGGCCTTCA AAGAAATCTA CTGAGAGTCA AAATAAGGAT 
AAAACTGTAG AAGAGGAAAA AGATGATGGA AAGCGACGGG GAAGAACCCC TCTGAAGTTG 
AAGAAACCAG CTCACAGGAG AAAGGCAGAG ATTCAGAGAG AAGAGCTGAA GGGAGATTCG 
GAGTCTGAGA CGACTGATGG ACGGTGTTTG AGAAGGTCTC CCAGAATCTG CAGACCAACC 
GCTAAAGCAG TGGAGATCCA GGATAAGAGG GCGGAGCGGA AACAGGCCAC GCCAGAGAAG 
GAGAAGGAGG AAGAAGAAGA AGAAGAGGAG GAGGAGCCTG TACAGAGGAA ACCGAGGAAA 
GCAGAGCCAG ATGGACAGAC AAAACCAAAG CAGGTGGGGA GACGTCGAAA ACGACAACCG 
TGGTCTCGCA TGAGGCGTAA AAAGAAATCT TCGGAGGATG AGGAAGATGA AGAGGAGAGC 
GATAGCGAAG AAGAGGAGAC AGAGGAGGAT GACAGCGATG AAGACTACAA GGTGGAGAAA 
AGCAAGAAGA GAAGGAGAAA CCGAAACCGC GAACGGCGCA GCTCTGACTC CTCCACCTCT 
TCATCAGAAG ACGATACACC CAATGACGAC CCCTGCAAAC ACTGTGGCCT TCCCAATCAT 
CCCGAACTGA TCCTGCTGTG TGATTCCTGT GACAGCGGCT ACCACACCGC CTGCCTGCGT 
CCTCCGCTCA TGATCATTCC AGACGGAGAG TGGTTCTGTC CTCCCTGCCA GCACAAGCTA 
CTTTGCGACA GACTGGAAGA GCAGCTGCTG AATTTGGATG CCGCCCTCAA AAAGAAAGAA 
CGAGCAGAGC GAAGGAAAGA GCGACTGGTG TATGTTGGCA TTAGTGTGGA GAACATCATC 
ACGCCCTCTG TTGAAGTGGA GGAAGAAAAG GAAGAGGTTT TGGTTAAGGA TAAAGCGGAG 
ACGAAGAAGA GCAAAGGCTG GGGACGGAGA TCAACCAGAG CCAAGAAATA CATAAGCTAC 
AGATTCGATG AGTTTGATGA AGCGATCGAA GAGGCGATTG AGGAGGACAT TAAAGAGGCA 
GAAGGTGGAG GAGCAGGACG AGGGAAGGAC ATGGCGAACA TCACAGGCCA CAGAGGGAAG 
GACATGTCCA CCATCCTGCA GGAGGAAGGC AAAGAGAACG GCCGACCACA AAGAACTAGC 
GCAGCGCAGA AGAAGAAACG GCGGCGTCTC AATGATCTGG ACAGCGACAG CACTGTAGAT 
GAGGAGGAGA GCGAAGATGA GTTTCGCCTC AGCGACAGCA GTTCTGAGGA GGAGTTTGTG 
GCGTCTGAAA ACGCTGAAAG TGAAGACAAT GCGCCATCCT TAGAGGACAG CGACTTTGAC 
AGTGATGCTC AAGGTTCACG AGTGAATCGT GCGAACAGAA GGAAAACGAG CCGGAGTCGA 
ATCCGGAGAA AACAACCTCC GCGCAGGAGA CGCAGACCCA GAGGATACTC TGATGAAGAG 
GAGCTGGAGA CTGATGAAGA GGAGGAGGAA GAGGACGAGA TGGTGACGGA GGGCTCTAGT 
GAGTTCAGTG ACAGTGATCT GGACGTCCGC TGTCGACGCT CGTATCGCAG CCGAAAGCAG 
GAGGTGAATT ACTGTGAAAC TTCAGATTCA GACGGCTCGC AAGCCAGCAC CAACAAAGAC 
AAGAAAAAGA AGCGCAGACG ACTGTCCAGC TCTGACAGCG AAGCCAGTTT CCAGTCAGCG 
GAATCGGAAG AAGAAGAGCG AAAACCCAAG AAGATCAGAG CCGACTCGTC TGAGGAGGAG 
GAATCCAGGA AGCGACGCAG ACGTTTATCC CTAAAGCGCC GACGGGAGTC AGAGGACGAT 
GACGATGATG ATGATTCAGA TGAATCCGAG GAAGAGGAGC GTCCTGTCCG GAAGCGAGTA 
AACCGAATCG ACTCTGACGA AAGTGAGAGC GAAGAAGAGG AGAAGGAGGT GAAGAAGAGT 
CCGCAGGAGA AAGACACAGA AGAGACGAGC GCTAGTAAAG GTCCCAGTCC AATAAACGAC 
AACACAGCGG AGCCTCCTTC TACTAATGGA CAGAGCCCTA TAAAGGGTCT GGAGGGCGTC 
GCGCCACGGC CGGGAGCAGG AGCAACGCCT AAAAATGGCA ACACACCATC CATAGCACCC 
AATGGCCTGG AAGCCGGCGC ACAGGAAGAA GACGAAGACG ATCTTTTGGG CGTCACAGAC 
CTCGTAGACT ATGTCTGCAA CAGCGAACAG TTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213567.1
CDS	95..5830
Translation	MSASEEADGSSPGLCPSFAVICSFLERYGALLDLPEVSFPELERSLQDSSAVPKLLVDLHVKLLRKIGKSVSAERWEKYLIKICQEFNTTWAWELEKKGYQELTVECKTGILKYLCECQFDDNVKFKNAINEEDPDKMRLQPIGRDKDGLMYWFQLDQDHNVRIYVEEQDDLDGSSWKCIVRNRDDLAQILGLLKTQIDPALLVKKEQEEGSSNNSPNPDDEENKKKEGAEVEKSQDDLSKSENKENILEKENKKEEGADATDALSEKGLKVKEELQKHSETANGSDVIAGSIKEEPQDDEKVFSAPSKKAEQTEEVKRKTLEETQRALKNDHQAKIPLKKREMKLSEDFDSNSAGLRNAPVKESRTCFDDSMSSAEKINGHVPHLQKEATDSESLSHFESNEKTKDAQVDRKMDPNETRFPSEEKMEVEKPKEKNEASTKQHKTTPGEEKPSELSEKTPDSEVVTENMETIEDIKTTPCDDTANELPKEKSIKDIPNKESKEDSLKDAQSKESKGESLKGSKESKGESLLKDAPSKESKEESLKDAPSKESKESSSKGAPSKESKGELFKESKESKKESLKDTPSKESIGESLRDTQSKESKESSSKDAPRKESKGESLKDAPSKESKEDSMKDTPSKESNESLLKESKESKEESLKDNPSKESKEEILKDTPSKESKQESLKDTPSKESKGESLKDAPSKESKESLLKESKESKEESLKDPPGKESAKSKDDKTKALSKKESLEDISNEKSAKSLKDDATKDPSKKESLQDISNESTKSVSKDDAAQDHFNKESSKDLASEESGKDQPAKDLSNEESIKNPPNQKPSKDLAKKESQKVIPESESQKVVSEKDLQKGIPEKGSQKSNPEKESQKSNPEKESQKSNPEKESQKSNPEKESQKSNPEKESQKSNPEKESQKSNPEKESQNVLPSQKSPNKDQEQAMETESFQSSKSVETREIIKPHSDTEKPENAEKTKLHESNTEKESTQKPCISEGDKLLTRGESIGSKPCEDTDNPQDKKQEKSGSSKSRTDTNKSSVSDSKKSETRKKVDAEDKCDAAETAKSDKSEIAEDKDKKSESTKTSDEVKAPEKPNKSDKEHEKQPNPMETDSEIPKTAEETTKESVKEANATVDNCTSKQSERDATSEEREASGVVQEKGKGIKKPGRPSKKSTESQNKDKTVEEEKDDGKRRGRTPLKLKKPAHRRKAEIQREELKGDSESETTDGRCLRRSPRICRPTAKAVEIQDKRAERKQATPEKEKEEEEEEEEEEPVQRKPRKAEPDGQTKPKQVGRRRKRQPWSRMRRKKKSSEDEEDEEESDSEEEETEEDDSDEDYKVEKSKKRRRNRNRERRSSDSSTSSSEDDTPNDDPCKHCGLPNHPELILLCDSCDSGYHTACLRPPLMIIPDGEWFCPPCQHKLLCDRLEEQLLNLDAALKKKERAERRKERLVYVGISVENIITPSVEVEEEKEEVLVKDKAETKKSKGWGRRSTRAKKYISYRFDEFDEAIEEAIEEDIKEAEGGGAGRGKDMANITGHRGKDMSTILQEEGKENGRPQRTSAAQKKKRRRLNDLDSDSTVDEEESEDEFRLSDSSSEEEFVASENAESEDNAPSLEDSDFDSDAQGSRVNRANRRKTSRSRIRRKQPPRRRRRPRGYSDEEELETDEEEEEEDEMVTEGSSEFSDSDLDVRCRRSYRSRKQEVNYCETSDSDGSQASTNKDKKKKRRRLSSSDSEASFQSAESEEEERKPKKIRADSSEEEESRKRRRRLSLKRRRESEDDDDDDDSDESEEEERPVRKRVNRIDSDESESEEEEKEVKKSPQEKDTEETSASKGPSPINDNTAEPPSTNGQSPIKGLEGVAPRPGAGATPKNGNTPSIAPNGLEAGAQEEDEDDLLGVTDLVDYVCNSEQL

Target ORF information:

RefSeq Version	XM_017358078.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio remodeling and spacing factor 1b, tandem duplicate 1 (rsf1b.1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358078.2

ORF Insert Sequence:

ATGTCTGCCT CGGAGGAAGC GGACGGTTCT TCCCCGGGAT TGTGCCCGAG CTTTGCCGTG 
ATCTGCTCGT TTCTGGAGCG GTACGGAGCT CTGCTGGACC TGCCGGAGGT GTCGTTCCCG 
GAGCTGGAGC GCAGCCTGCA GGACAGCAGC GCGGTACCCA AACTTCTTGT GGACCTTCAC 
GTGAAGTTAC TGAGGAAAAT TGGCAAGTCT GTGTCTGCAG AGAGATGGGA GAAATATCTC 
ATTAAGATTT GTCAGGAGTT TAACACCACA TGGGCATGGG AGTTGGAAAA GAAAGGTTAT 
CAAGAGTTGA CAGTGGAGTG TAAGACAGGA ATATTGAAGT ATTTGTGTGA ATGCCAGTTT 
GATGACAATG TCAAGTTTAA AAATGCTATC AATGAAGAAG ACCCGGATAA AATGCGACTT 
CAGCCTATTG GCCGAGACAA AGACGGCCTC ATGTACTGGT TCCAGCTGGA CCAAGACCAT 
AATGTAAGGA TCTATGTGGA AGAGCAGGAT GATTTGGATG GGTCGTCCTG GAAATGTATA 
GTAAGAAATA GAGATGACCT GGCTCAGATT CTTGGGTTGT TGAAGACTCA GATTGATCCA 
GCACTCCTGG TGAAGAAGGA ACAGGAGGAG GGCTCGTCTA ATAACAGTCC AAATCCCGAT 
GATGAGGAGA ACAAAAAGAA AGAGGGAGCT GAAGTGGAGA AGTCACAAGA TGATTTGTCC 
AAATCTGAAA ATAAGGAAAA CATATTAGAG AAAGAAAATA AAAAGGAAGA AGGTGCAGAT 
GCCACAGATG CTTTGTCGGA AAAGGGTTTA AAGGTAAAAG AGGAGCTGCA GAAACATTCA 
GAGACGGCAA ACGGATCTGA CGTTATTGCT GGATCAATCA AAGAAGAACC ACAGGATGAT 
GAGAAAGTCT TTTCCGCACC TTCAAAGAAA GCAGAACAGA CAGAGGAGGT GAAGAGAAAA 
ACACTAGAAG AGACCCAAAG AGCACTGAAA AATGACCATC AAGCTAAAAT CCCCCTGAAG 
AAGCGAGAGA TGAAGCTAAG TGAAGACTTT GACAGCAACA GCGCAGGACT TCGAAATGCT 
CCAGTAAAAG AGTCAAGGAC GTGTTTCGAT GATAGCATGT CGAGTGCTGA AAAGATCAAT 
GGCCATGTTC CTCACCTTCA GAAAGAAGCA ACAGATTCAG AAAGTCTCTC TCATTTTGAA 
TCCAATGAAA AAACAAAAGA CGCCCAGGTG GATAGAAAGA TGGACCCGAA TGAAACCAGA 
TTTCCTTCTG AAGAGAAGAT GGAAGTAGAG AAACCTAAGG AGAAAAATGA AGCTTCAACT 
AAACAGCACA AAACAACTCC TGGGGAAGAA AAACCAAGTG AACTATCAGA AAAGACACCT 
GATTCTGAAG TAGTAACTGA GAATATGGAA ACTATTGAAG ATATTAAGAC AACACCATGT 
GACGACACAG CAAATGAACT GCCTAAAGAG AAATCAATTA AAGATATTCC AAACAAAGAA 
TCAAAAGAAG ATTCATTGAA AGATGCACAA AGCAAAGAAT CAAAGGGAGA GTCATTGAAA 
GGAAGCAAAG AATCAAAAGG AGAATCATTA TTAAAAGATG CCCCAAGCAA AGAATCAAAA 
GAAGAATCAT TGAAAGATGC GCCGAGCAAA GAGTCAAAAG AATCATCATC AAAAGGTGCC 
CCAAGCAAAG AATCAAAAGG AGAATTATTT AAGGAAAGCA AAGAGTCGAA AAAAGAATCA 
TTAAAAGATA CCCCAAGTAA AGAATCAATA GGAGAATCAT TGAGAGATAC CCAAAGCAAA 
GAGTCAAAAG AATCATCATC AAAAGATGCC CCACGCAAAG AATCAAAAGG AGAATCATTG 
AAGGATGCCC CAAGCAAAGA GTCAAAAGAA GACTCAATGA AAGATACCCC AAGCAAAGAA 
TCAAATGAAT CATTATTGAA GGAAAGCAAA GAGTCAAAAG AAGAATCATT GAAAGATAAC 
CCAAGTAAAG AATCAAAAGA AGAAATATTG AAAGATACCC CAAGCAAAGA ATCAAAACAA 
GAATCATTGA AAGATACCCC AAGCAAAGAA TCAAAGGGAG AATCGTTGAA AGATGCCCCA 
AGCAAAGAAT CAAAAGAATC ATTATTGAAG GAAAGTAAAG AGTCAAAAGA AGAATCGTTG 
AAAGATCCCC CAGGCAAGGA GTCAGCAAAA TCAAAAGATG ACAAAACTAA AGCTCTTTCA 
AAAAAGGAAT CATTGGAAGA TATCTCAAAT GAAAAGTCAG CAAAATCTCT CAAAGATGAT 
GCAACAAAAG ATCCATCAAA AAAAGAATCA TTGCAAGATA TCTCAAACGA GTCAACAAAA 
TCTGTATCAA AAGACGACGC AGCACAAGAT CACTTTAACA AAGAATCATC AAAAGATCTC 
GCAAGCGAAG AGTCGGGGAA AGATCAGCCG GCAAAAGATC TTTCGAATGA AGAGTCTATA 
AAAAATCCTC CAAATCAAAA ACCAAGCAAA GATCTTGCAA AAAAAGAGTC ACAAAAGGTT 
ATCCCAGAAA GCGAATCACA AAAAGTTGTT TCAGAGAAAG ATTTGCAGAA GGGCATCCCA 
GAAAAAGGGT CACAGAAGAG TAACCCGGAA AAAGAGTCGC AGAAGAGTAA CCCGGAAAAA 
GAGTCGCAGA AGAGTAACCC GGAAAAAGAG TCGCAGAAGA GTAACCCGGA AAAAGAGTCG 
CAGAAGAGTA ACCCGGAAAA AGAGTCGCAG AAGAGTAACC CGGAAAAAGA GTCGCAGAAG 
AGTAACCCGG AAAAAGAGTC GCAGAATGTT CTCCCAAGTC AGAAATCTCC TAATAAAGAC 
CAAGAACAAG CAATGGAAAC CGAATCATTC CAGTCATCAA AATCAGTAGA GACCCGTGAA 
ATAATCAAAC CTCATTCTGA CACAGAAAAG CCAGAGAATG CTGAAAAAAC TAAGCTTCAT 
GAAAGCAACA CAGAAAAAGA GTCAACTCAA AAACCATGCA TCTCTGAAGG AGACAAACTC 
TTAACCAGGG GAGAGTCTAT TGGTTCTAAA CCCTGTGAAG ATACGGATAA CCCTCAAGAC 
AAAAAACAAG AGAAGTCTGG CTCCTCCAAA TCGAGAACAG ATACTAATAA ATCATCTGTT 
TCTGATTCAA AAAAGTCAGA AACCAGAAAG AAAGTAGATG CTGAGGACAA ATGTGATGCA 
GCTGAAACGG CTAAAAGTGA CAAATCAGAA ATAGCAGAAG ACAAAGATAA AAAGAGTGAA 
AGTACAAAAA CATCAGATGA AGTTAAAGCT CCAGAAAAGC CCAATAAATC AGATAAAGAA 
CATGAAAAGC AACCAAATCC AATGGAAACT GATTCAGAGA TCCCTAAAAC TGCTGAGGAG 
ACAACAAAAG AAAGCGTGAA GGAAGCCAAT GCTACTGTAG ATAACTGCAC CAGCAAACAA 
TCAGAGCGAG ACGCGACTTC TGAAGAACGC GAGGCTTCCG GTGTTGTTCA AGAAAAGGGG 
AAAGGCATTA AAAAACCAGG ACGGCCTTCA AAGAAATCTA CTGAGAGTCA AAATAAGGAT 
AAAACTGTAG AAGAGGAAAA AGATGATGGA AAGCGACGGG GAAGAACCCC TCTGAAGTTG 
AAGAAACCAG CTCACAGGAG AAAGGCAGAG ATTCAGAGAG AAGAGCTGAA GGGAGATTCG 
GAGTCTGAGA CGACTGATGG ACGGTGTTTG AGAAGGTCTC CCAGAATCTG CAGACCAACC 
GCTAAAGCAG TGGAGATCCA GGATAAGAGG GCGGAGCGGA AACAGGCCAC GCCAGAGAAG 
GAGAAGGAGG AAGAAGAAGA AGAAGAGGAG GAGGAGCCTG TACAGAGGAA ACCGAGGAAA 
GCAGAGCCAG ATGGACAGAC AAAACCAAAG CAGGTGGGGA GACGTCGAAA ACGACAACCG 
TGGTCTCGCA TGAGGCGTAA AAAGAAATCT TCGGAGGATG AGGAAGATGA AGAGGAGAGC 
GATAGCGAAG AAGAGGAGAC AGAGGAGGAT GACAGCGATG AAGACTACAA GGTGGAGAAA 
AGCAAGAAGA GAAGGAGAAA CCGAAACCGC GAACGGCGCA GCTCTGACTC CTCCACCTCT 
TCATCAGAAG ACGATACACC CAATGACGAC CCCTGCAAAC ACTGTGGCCT TCCCAATCAT 
CCCGAACTGA TCCTGCTGTG TGATTCCTGT GACAGCGGCT ACCACACCGC CTGCCTGCGT 
CCTCCGCTCA TGATCATTCC AGACGGAGAG TGGTTCTGTC CTCCCTGCCA GCACAAGCTA 
CTTTGCGACA GACTGGAAGA GCAGCTGCTG AATTTGGATG CCGCCCTCAA AAAGAAAGAA 
CGAGCAGAGC GAAGGAAAGA GCGACTGGTG TATGTTGGCA TTAGTGTGGA GAACATCATC 
ACGCCCTCTG TTGAAGTGGA GGAAGAAAAG GAAGAGGTTT TGGTTAAGGA TAAAGCGGAG 
ACGAAGAAGA GCAAAGGCTG GGGACGGAGA TCAACCAGAG CCAAGAAATA CATAAGCTAC 
AGATTCGATG AGTTTGATGA AGCGATCGAA GAGGCGATTG AGGAGGACAT TAAAGAGGCA 
GAAGGTGGAG GAGCAGGACG AGGGAAGGAC ATGGCGAACA TCACAGGCCA CAGAGGGAAG 
GACATGTCCA CCATCCTGCA GGAGGAAGGC AAAGAGAACG GCCGACCACA AAGAACTAGC 
GCAGCGCAGA AGAAGAAACG GCGGCGTCTC AATGATCTGG ACAGCGACAG CACTGTAGAT 
GAGGAGGAGA GCGAAGATGA GTTTCGCCTC AGCGACAGCA GTTCTGAGGA GGAGTTTGTG 
GCGTCTGAAA ACGCTGAAAG TGAAGACAAT GCGCCATCCT TAGAGGACAG CGACTTTGAC 
AGTGATGCTC AAGGTTCACG AGTGAATCGT GCGAACAGAA GGAAAACGAG CCGGAGTCGA 
ATCCGGAGAA AACAACCTCC GCGCAGGAGA CGCAGACCCA GAGGATACTC TGATGAAGAG 
GAGCTGGAGA CTGATGAAGA GGAGGAGGAA GAGGACGAGA TGGTGACGGA GGGCTCTAGT 
GAGTTCAGTG ACAGTGATCT GGACGTCCGC TGTCGACGCT CGTATCGCAG CCGAAAGCAG 
GAGGTGAATT ACTGTGAAAC TTCAGATTCA GACGGCTCGC AAGCCAGCAC CAACAAAGAC 
AAGAAAAAGA AGCGCAGACG ACTGTCCAGC TCTGACAGCG AAGCCAGTTT CCAGTCAGCG 
GAATCGGAAG AAGAAGAGCG AAAACCCAAG AAGATCAGAG CCGACTCGTC TGAGGAGGAG 
GAATCCAGGA AGCGACGCAG ACGTTTATCC CTAAAGCGCC GACGGGAGTC AGAGGACGAT 
GACGATGATG ATGATTCAGA TGAATCCGAG GAAGAGGAGC GTCCTGTCCG GAAGCGAGTA 
AACCGAATCG ACTCTGACGA AAGTGAGAGC GAAGAAGAGG AGAAGGAGGT GAAGAAGAGT 
CCGCAGGAGA AAGACACAGA AGAGACGAGC GCTAGTAAAG GTCCCAGTCC AATAAACGAC 
AACACAGCGG AGCCTCCTTC TACTAATGGA CAGAGCCCTA TAAAGGGTCT GGAGGGCGTC 
GCGCCACGGC CGGGAGCAGG AGCAACGCCT AAAAATGGCA ACACACCATC CATAGCACCC 
AATGGCCTGG AAGCCGGCGC ACAGGAAGAA GACGAAGACG ATCTTTTGGG CGTCACAGAC 
CTCGTAGACT ATGTCTGCAA CAGCGAACAG TTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rsf1b.1 ( XM_017358080.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213569.1 Danio rerio remodeling and spacing factor 1b, tandem duplicate 1 (rsf1b.1), transcript variant X3, mRNA.

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CloneID	ODa16391
Clone ID Related Accession (Same CDS sequence)	XM_017358080.1 , XM_017358080.2
Accession Version	XM_017358080.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	remodeling and spacing factor 1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCCT CGGAGGAAGC GGACGGTTCT TCCCCGGGAT TGTGCCCGAG CTTTGCCGTG 
ATCTGCTCGT TTCTGGAGCG GTACGGAGCT CTGCTGGACC TGCCGGAGGT GTCGTTCCCG 
GAGCTGGAGC GCAGCCTGCA GGACAGCAGC GCGGTACCCA AACTTCTTGT GGACCTTCAC 
GTGAAGTTAC TGAGGAAAAT TGGCAAGTCT GTGTCTGCAG AGAGATGGGA GAAATATCTC 
ATTAAGATTT GTCAGGAGTT TAACACCACA TGGGCATGGG AGTTGGAAAA GAAAGGTTAT 
CAAGAGTTGA CAGTGGAGTG TAAGACAGGA ATATTGAAGT ATTTGTGTGA ATGCCAGTTT 
GATGACAATG TCAAGTTTAA AAATGCTATC AATGAAGAAG ACCCGGATAA AATGCGACTT 
CAGCCTATTG GCCGAGACAA AGACGGCCTC ATGTACTGGT TCCAGCTGGA CCAAGACCAT 
AATGTAAGGA TCTATGTGGA AGAGCAGGAT GATTTGGATG GGTCGTCCTG GAAATGTATA 
GTAAGAAATA GAGATGACCT GGCTCAGATT CTTGGGTTGT TGAAGACTCA GATTGATCCA 
GCACTCCTGG TGAAGAAGGA ACAGGAGGAG GGCTCGTCTA ATAACAGTCC AAATCCCGAT 
GATGAGGAGA ACAAAAAGAA AGAGGGAGCT GAAGTGGAGA AGTCACAAGA TGATTTGTCC 
AAATCTGAAA ATAAGGAAAA CATATTAGAG AAAGAAAATA AAAAGGAAGA AGGTGCAGAT 
GCCACAGATG CTTTGTCGGA AAAGGGTTTA AAGGTAAAAG AGGAGCTGCA GAAACATTCA 
GAGACGGCAA ACGGATCTGA CGTTATTGCT GGATCAATCA AAGAAGAACC ACAGGATGAT 
GAGAAAGTCT TTTCCGCACC TTCAAAGAAA GCAGAACAGA CAGAGGAGGT GAAGAGAAAA 
ACACTAGAAG AGACCCAAAG AGCACTGAAA AATGACCATC AAGCTAAAAT CCCCCTGAAG 
AAGCGAGAGA TGAAGCTAAG TGAAGACTTT GACAGCAACA GCGCAGGACT TCGAAATGCT 
CCAGTAAAAG AGTCAAGGAC GTGTTTCGAT GATAGCATGT CGAGTGCTGA AAAGATCAAT 
GGCCATGTTC CTCACCTTCA GAAAGAAGCA ACAGATTCAG AAAGTCTCTC TCATTTTGAA 
TCCAATGAAA AAACAAAAGA CGCCCAGGTG GATAGAAAGA TGGACCCGAA TGAAACCAGA 
TTTCCTTCTG AAGAGAAGAT GGAAGTAGAG AAACCTAAGG AGAAAAATGA AGCTTCAACT 
AAACAGCACA AAACAACTCC TGGGGAAGAA AAACCAAGTG AACTATCAGA AAAGACACCT 
GATTCTGAAG TAGTAACTGA GAATATGGAA ACTATTGAAG ATATTAAGAC AACACCATGT 
GACGACACAG CAAATGAACT GCCTAAAGAG AAATCAATTA AAGATATTCC AAACAAAGAA 
TCAAAAGAAG ATTCATTGAA AGATGCACAA AGCAAAGAAT CAAAGGGAGA GTCATTGAAA 
GGAAGCAAAG AATCAAAAGG AGAATCATTA TTAAAAGATG CCCCAAGCAA AGAATCAAAA 
GAAGAATCAT TGAAAGATGC GCCGAGCAAA GAGTCAAAAG AATCATCATC AAAAGGTGCC 
CCAAGCAAAG AATCAAAAGG AGAATTATTT AAGGAAAGCA AAGAGTCGAA AAAAGAATCA 
TTAAAAGATA CCCCAAGTAA AGAATCAATA GGAGAATCAT TGAGAGATAC CCAAAGCAAA 
GAGTCAAAAG AATCATCATC AAAAGATGCC CCACGCAAAG AATCAAAAGG AGAATCATTG 
AAGGATGCCC CAAGCAAAGA GTCAAAAGAA GACTCAATGA AAGATACCCC AAGCAAAGAA 
TCAAATGAAT CATTATTGAA GGAAAGCAAA GAGTCAAAAG AAGAATCATT GAAAGATAAC 
CCAAGTAAAG AATCAAAAGA AGAAATATTG AAAGATACCC CAAGCAAAGA ATCAAAACAA 
GAATCATTGA AAGATACCCC AAGCAAAGAA TCAAAGGGAG AATCGTTGAA AGATGCCCCA 
AGCAAAGAAT CAAAAGAATC ATTATTGAAG GAAAGTAAAG AGTCAAAAGA AGAATCGTTG 
AAAGATCCCC CAGGCAAGGA GTCAGCAAAA TCAAAAGATG ACAAAACTAA AGCTCTTTCA 
AAAAAGGAAT CATTGGAAGA TATCTCAAAT GAAAAGTCAG CAAAATCTCT CAAAGATGAT 
GCAACAAAAG ATCCATCAAA AAAAGAATCA TTGCAAGATA TCTCAAACGA GTCAACAAAA 
TCTGTATCAA AAGACGACGC AGCACAAGAT CACTTTAACA AAGAATCATC AAAAGATCTC 
GCAAGCGAAG AGTCGGGGAA AGATCAGCCG GCAAAAGATC TTTCGAATGA AGAGTCTATA 
AAAAATCCTC CAAATCAAAA ACCAAGCAAA GATCTTGCAA AAAAAGAGTC ACAAAAGGTT 
ATCCCAGAAA GCGAATCACA AAAAGTTGTT TCAGAGAAAG ATTTGCAGAA GGGCATCCCA 
GAAAAAGGGT CACAGAAGAG TAACCCGGAA AAAGAGTCGC AGAAGAGTAA CCCGGAAAAA 
GAGTCGCAGA AGAGTAACCC GGAAAAAGAG TCGCAGAAGA GTAACCCGGA AAAAGAGTCG 
CAGAAGAGTA ACCCGGAAAA AGAGTCGCAG AAGAGTAACC CGGAAAAAGA GTCGCAGAAG 
AGTAACCCGG AAAAAGAGTC GCAGAATGTT CTCCCAAGTC AGAAATCTCC TAATAAAGAC 
CAAGAACAAG CAATGGAAAC CGAATCATTC CAGTCATCAA AATCAGTAGA GACCCGTGAA 
ATAATCAAAC CTCATTCTGA CACAGAAAAG CCAGAGAATG CTGAAAAAAC TAAGCTTCAT 
GAAAGCAACA CAGAAAAAGA GTCAACTCAA AAACCATGCA TCTCTGAAGG AGACAAACTC 
TTAACCAGGG GAGAGTCTAT TGGTTCTAAA CCCTGTGAAG ATACGGATAA CCCTCAAGAC 
AAAAAACAAG AGAAGTCTGG CTCCTCCAAA TCGAGAACAG ATACTAATAA ATCATCTGTT 
TCTGATTCAA AAAAGTCAGA AACCAGAAAG AAAGTAGATG CTGAGGACAA ATGTGATGCA 
GCTGAAACGG CTAAAAGTGA CAAATCAGAA ATAGCAGAAG ACAAAGATAA AAAGAGTGAA 
AGTACAAAAA CATCAGATGA AGTTAAAGCT CCAGAAAAGC CCAATAAATC AGATAAAGAA 
CATGAAAAGC AACCAAATCC AATGGAAACT GATTCAGAGA TCCCTAAAAC TGCTGAGGAG 
ACAACAAAAG AAAGCGTGAA GGAAGCCAAT GCTACTGTAG ATAACTGCAC CAGCAAACAA 
TCAGAGCGAG ACGCGACTTC TGAAGAACGC GAGGCTTCCG GTGTTGTTCA AGAAAAGGGG 
AAAGGCATTA AAAAACCAGG ACGGCCTTCA AAGAAATCTA CTGAGAGTCA AAATAAGGAT 
AAAACTGTAG AAGAGGAAAA AGATGATGGA AAGCGACGGG GAAGAACCCC TCTGAAGTTG 
AAGAAACCAG CTCACAGGAG AAAGGCAGAG ATTCAGAGAG AAGAGCTGAA GGGAGATTCG 
GAGTCTGAGA CGACTGATGG ACGGTGTTTG AGAAGGTCTC CCAGAATCTG CAGACCAACC 
GCTAAAGCAG TGGAGATCCA GGATAAGAGG GCGGAGCGGA AACAGGCCAC GCCAGAGAAG 
GAGAAGGAGG AAGAAGAAGA AGAAGAGGAG GAGGAGCCTG TACAGAGGAA ACCGAGGAAA 
GCAGAGCCAG ATGGACAGAC AAAACCAAAG GTGGGGAGAC GTCGAAAACG ACAACCGTGG 
TCTCGCATGA GGCGTAAAAA GAAATCTTCG GAGGATGAGG AAGATGAAGA GGAGAGCGAT 
AGCGAAGAAG AGGAGACAGA GGAGGATGAC AGCGATGAAG ACTACAAGGT GGAGAAAAGC 
AAGAAGAGAA GGAGAAACCG AAACCGCGAA CGGCGCAGCT CTGACTCCTC CACCTCTTCA 
TCAGAAGACG ATACACCCAA TGACGACCCC TGCAAACACT GTGGCCTTCC CAATCATCCC 
GAACTGATCC TGCTGTGTGA TTCCTGTGAC AGCGGCTACC ACACCGCCTG CCTGCGTCCT 
CCGCTCATGA TCATTCCAGA CGGAGAGTGG TTCTGTCCTC CCTGCCAGCA CAAGCTACTT 
TGCGACAGAC TGGAAGAGCA GCTGCTGAAT TTGGATGCCG CCCTCAAAAA GAAAGAACGA 
GCAGAGCGAA GGAAAGAGCG ACTGGTGTAT GTTGGCATTA GTGTGGAGAA CATCATCACG 
CCCTCTGTTG AAGTGGAGGA AGAAAAGGAA GAGGTTTTGG TTAAGGATAA AGCGGAGACG 
AAGAAGAGCA AAGGCTGGGG ACGGAGATCA ACCAGAGCCA AGAAATACAT AAGCTACAGA 
TTCGATGAGT TTGATGAAGC GATCGAAGAG GCGATTGAGG AGGACATTAA AGAGGCAGAA 
GGTGGAGGAG CAGGACGAGG GAAGGACATG GCGAACATCA CAGGCCACAG AGGGAAGGAC 
ATGTCCACCA TCCTGCAGGA GGAAGGCAAA GAGAACGGCC GACCACAAAG AACTAGCGCA 
GCGCAGAAGA AGAAACGGCG GCGTCTCAAT GATCTGGACA GCGACAGCAC TGTAGATGAG 
GAGGAGAGCG AAGATGAGTT TCGCCTCAGC GACAGCAGTT CTGAGGAGGA GTTTGTGGCG 
TCTGAAAACG CTGAAAGTGA AGACAATGCG CCATCCTTAG AGGACAGCGA CTTTGACAGT 
GATGCTCAAG GTTCACGAGT GAATCGTGCG AACAGAAGGA AAACGAGCCG GAGTCGAATC 
CGGAGAAAAC AACCTCCGCG CAGGAGACGC AGACCCAGAG GATACTCTGA TGAAGAGGAG 
CTGGAGACTG ATGAAGAGGA GGAGGAAGAG GACGAGATGG TGACGGAGGG CTCTAGTGAG 
TTCAGTGACA GTGATCTGGA CGTCCGCTGT CGACGCTCGT ATCGCAGCCG AAAGCAGGAG 
GTGAATTACT GTGAAACTTC AGATTCAGAC GGCTCGCAAG CCAGCACCAA CAAAGACAAG 
AAAAAGAAGC GCAGACGACT GTCCAGCTCT GACAGCGAAG CCAGTTTCCA GTCAGCGGAA 
TCGGAAGAAG AAGAGCGAAA ACCCAAGAAG ATCAGAGCCG ACTCGTCTGA GGAGGAGGAA 
TCCAGGAAGC GACGCAGACG TTTATCCCTA AAGCGCCGAC GGGAGTCAGA GGACGATGAC 
GATGATGATG ATTCAGATGA ATCCGAGGAA GAGGAGCGTC CTGTCCGGAA GCGAGTAAAC 
CGAATCGACT CTGACGAAAG TGAGAGCGAA GAAGAGGAGA AGGAGGTGAA GAAGAGTCCG 
CAGGAGAAAG ACACAGAAGA GACGAGCGCT AGTAAAGGTC CCAGTCCAAT AAACGACAAC 
ACAGCGGAGC CTCCTTCTAC TAATGGACAG AGCCCTATAA AGGGTCTGGA GGGCGTCGCG 
CCACGGCCGG GAGCAGGAGC AACGCCTAAA AATGGCAACA CACCATCCAT AGCACCCAAT 
GGCCTGGAAG CCGGCGCACA GGAAGAAGAC GAAGACGATC TTTTGGGCGT CACAGACCTC 
GTAGACTATG TCTGCAACAG CGAACAGTTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213569.1
CDS	52..5784
Translation	MSASEEADGSSPGLCPSFAVICSFLERYGALLDLPEVSFPELERSLQDSSAVPKLLVDLHVKLLRKIGKSVSAERWEKYLIKICQEFNTTWAWELEKKGYQELTVECKTGILKYLCECQFDDNVKFKNAINEEDPDKMRLQPIGRDKDGLMYWFQLDQDHNVRIYVEEQDDLDGSSWKCIVRNRDDLAQILGLLKTQIDPALLVKKEQEEGSSNNSPNPDDEENKKKEGAEVEKSQDDLSKSENKENILEKENKKEEGADATDALSEKGLKVKEELQKHSETANGSDVIAGSIKEEPQDDEKVFSAPSKKAEQTEEVKRKTLEETQRALKNDHQAKIPLKKREMKLSEDFDSNSAGLRNAPVKESRTCFDDSMSSAEKINGHVPHLQKEATDSESLSHFESNEKTKDAQVDRKMDPNETRFPSEEKMEVEKPKEKNEASTKQHKTTPGEEKPSELSEKTPDSEVVTENMETIEDIKTTPCDDTANELPKEKSIKDIPNKESKEDSLKDAQSKESKGESLKGSKESKGESLLKDAPSKESKEESLKDAPSKESKESSSKGAPSKESKGELFKESKESKKESLKDTPSKESIGESLRDTQSKESKESSSKDAPRKESKGESLKDAPSKESKEDSMKDTPSKESNESLLKESKESKEESLKDNPSKESKEEILKDTPSKESKQESLKDTPSKESKGESLKDAPSKESKESLLKESKESKEESLKDPPGKESAKSKDDKTKALSKKESLEDISNEKSAKSLKDDATKDPSKKESLQDISNESTKSVSKDDAAQDHFNKESSKDLASEESGKDQPAKDLSNEESIKNPPNQKPSKDLAKKESQKVIPESESQKVVSEKDLQKGIPEKGSQKSNPEKESQKSNPEKESQKSNPEKESQKSNPEKESQKSNPEKESQKSNPEKESQKSNPEKESQNVLPSQKSPNKDQEQAMETESFQSSKSVETREIIKPHSDTEKPENAEKTKLHESNTEKESTQKPCISEGDKLLTRGESIGSKPCEDTDNPQDKKQEKSGSSKSRTDTNKSSVSDSKKSETRKKVDAEDKCDAAETAKSDKSEIAEDKDKKSESTKTSDEVKAPEKPNKSDKEHEKQPNPMETDSEIPKTAEETTKESVKEANATVDNCTSKQSERDATSEEREASGVVQEKGKGIKKPGRPSKKSTESQNKDKTVEEEKDDGKRRGRTPLKLKKPAHRRKAEIQREELKGDSESETTDGRCLRRSPRICRPTAKAVEIQDKRAERKQATPEKEKEEEEEEEEEEPVQRKPRKAEPDGQTKPKVGRRRKRQPWSRMRRKKKSSEDEEDEEESDSEEEETEEDDSDEDYKVEKSKKRRRNRNRERRSSDSSTSSSEDDTPNDDPCKHCGLPNHPELILLCDSCDSGYHTACLRPPLMIIPDGEWFCPPCQHKLLCDRLEEQLLNLDAALKKKERAERRKERLVYVGISVENIITPSVEVEEEKEEVLVKDKAETKKSKGWGRRSTRAKKYISYRFDEFDEAIEEAIEEDIKEAEGGGAGRGKDMANITGHRGKDMSTILQEEGKENGRPQRTSAAQKKKRRRLNDLDSDSTVDEEESEDEFRLSDSSSEEEFVASENAESEDNAPSLEDSDFDSDAQGSRVNRANRRKTSRSRIRRKQPPRRRRRPRGYSDEEELETDEEEEEEDEMVTEGSSEFSDSDLDVRCRRSYRSRKQEVNYCETSDSDGSQASTNKDKKKKRRRLSSSDSEASFQSAESEEEERKPKKIRADSSEEEESRKRRRRLSLKRRRESEDDDDDDDSDESEEEERPVRKRVNRIDSDESESEEEEKEVKKSPQEKDTEETSASKGPSPINDNTAEPPSTNGQSPIKGLEGVAPRPGAGATPKNGNTPSIAPNGLEAGAQEEDEDDLLGVTDLVDYVCNSEQL

Target ORF information:

RefSeq Version	XM_017358080.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio remodeling and spacing factor 1b, tandem duplicate 1 (rsf1b.1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358080.1

ORF Insert Sequence:

ATGTCTGCCT CGGAGGAAGC GGACGGTTCT TCCCCGGGAT TGTGCCCGAG CTTTGCCGTG 
ATCTGCTCGT TTCTGGAGCG GTACGGAGCT CTGCTGGACC TGCCGGAGGT GTCGTTCCCG 
GAGCTGGAGC GCAGCCTGCA GGACAGCAGC GCGGTACCCA AACTTCTTGT GGACCTTCAC 
GTGAAGTTAC TGAGGAAAAT TGGCAAGTCT GTGTCTGCAG AGAGATGGGA GAAATATCTC 
ATTAAGATTT GTCAGGAGTT TAACACCACA TGGGCATGGG AGTTGGAAAA GAAAGGTTAT 
CAAGAGTTGA CAGTGGAGTG TAAGACAGGA ATATTGAAGT ATTTGTGTGA ATGCCAGTTT 
GATGACAATG TCAAGTTTAA AAATGCTATC AATGAAGAAG ACCCGGATAA AATGCGACTT 
CAGCCTATTG GCCGAGACAA AGACGGCCTC ATGTACTGGT TCCAGCTGGA CCAAGACCAT 
AATGTAAGGA TCTATGTGGA AGAGCAGGAT GATTTGGATG GGTCGTCCTG GAAATGTATA 
GTAAGAAATA GAGATGACCT GGCTCAGATT CTTGGGTTGT TGAAGACTCA GATTGATCCA 
GCACTCCTGG TGAAGAAGGA ACAGGAGGAG GGCTCGTCTA ATAACAGTCC AAATCCCGAT 
GATGAGGAGA ACAAAAAGAA AGAGGGAGCT GAAGTGGAGA AGTCACAAGA TGATTTGTCC 
AAATCTGAAA ATAAGGAAAA CATATTAGAG AAAGAAAATA AAAAGGAAGA AGGTGCAGAT 
GCCACAGATG CTTTGTCGGA AAAGGGTTTA AAGGTAAAAG AGGAGCTGCA GAAACATTCA 
GAGACGGCAA ACGGATCTGA CGTTATTGCT GGATCAATCA AAGAAGAACC ACAGGATGAT 
GAGAAAGTCT TTTCCGCACC TTCAAAGAAA GCAGAACAGA CAGAGGAGGT GAAGAGAAAA 
ACACTAGAAG AGACCCAAAG AGCACTGAAA AATGACCATC AAGCTAAAAT CCCCCTGAAG 
AAGCGAGAGA TGAAGCTAAG TGAAGACTTT GACAGCAACA GCGCAGGACT TCGAAATGCT 
CCAGTAAAAG AGTCAAGGAC GTGTTTCGAT GATAGCATGT CGAGTGCTGA AAAGATCAAT 
GGCCATGTTC CTCACCTTCA GAAAGAAGCA ACAGATTCAG AAAGTCTCTC TCATTTTGAA 
TCCAATGAAA AAACAAAAGA CGCCCAGGTG GATAGAAAGA TGGACCCGAA TGAAACCAGA 
TTTCCTTCTG AAGAGAAGAT GGAAGTAGAG AAACCTAAGG AGAAAAATGA AGCTTCAACT 
AAACAGCACA AAACAACTCC TGGGGAAGAA AAACCAAGTG AACTATCAGA AAAGACACCT 
GATTCTGAAG TAGTAACTGA GAATATGGAA ACTATTGAAG ATATTAAGAC AACACCATGT 
GACGACACAG CAAATGAACT GCCTAAAGAG AAATCAATTA AAGATATTCC AAACAAAGAA 
TCAAAAGAAG ATTCATTGAA AGATGCACAA AGCAAAGAAT CAAAGGGAGA GTCATTGAAA 
GGAAGCAAAG AATCAAAAGG AGAATCATTA TTAAAAGATG CCCCAAGCAA AGAATCAAAA 
GAAGAATCAT TGAAAGATGC GCCGAGCAAA GAGTCAAAAG AATCATCATC AAAAGGTGCC 
CCAAGCAAAG AATCAAAAGG AGAATTATTT AAGGAAAGCA AAGAGTCGAA AAAAGAATCA 
TTAAAAGATA CCCCAAGTAA AGAATCAATA GGAGAATCAT TGAGAGATAC CCAAAGCAAA 
GAGTCAAAAG AATCATCATC AAAAGATGCC CCACGCAAAG AATCAAAAGG AGAATCATTG 
AAGGATGCCC CAAGCAAAGA GTCAAAAGAA GACTCAATGA AAGATACCCC AAGCAAAGAA 
TCAAATGAAT CATTATTGAA GGAAAGCAAA GAGTCAAAAG AAGAATCATT GAAAGATAAC 
CCAAGTAAAG AATCAAAAGA AGAAATATTG AAAGATACCC CAAGCAAAGA ATCAAAACAA 
GAATCATTGA AAGATACCCC AAGCAAAGAA TCAAAGGGAG AATCGTTGAA AGATGCCCCA 
AGCAAAGAAT CAAAAGAATC ATTATTGAAG GAAAGTAAAG AGTCAAAAGA AGAATCGTTG 
AAAGATCCCC CAGGCAAGGA GTCAGCAAAA TCAAAAGATG ACAAAACTAA AGCTCTTTCA 
AAAAAGGAAT CATTGGAAGA TATCTCAAAT GAAAAGTCAG CAAAATCTCT CAAAGATGAT 
GCAACAAAAG ATCCATCAAA AAAAGAATCA TTGCAAGATA TCTCAAACGA GTCAACAAAA 
TCTGTATCAA AAGACGACGC AGCACAAGAT CACTTTAACA AAGAATCATC AAAAGATCTC 
GCAAGCGAAG AGTCGGGGAA AGATCAGCCG GCAAAAGATC TTTCGAATGA AGAGTCTATA 
AAAAATCCTC CAAATCAAAA ACCAAGCAAA GATCTTGCAA AAAAAGAGTC ACAAAAGGTT 
ATCCCAGAAA GCGAATCACA AAAAGTTGTT TCAGAGAAAG ATTTGCAGAA GGGCATCCCA 
GAAAAAGGGT CACAGAAGAG TAACCCGGAA AAAGAGTCGC AGAAGAGTAA CCCGGAAAAA 
GAGTCGCAGA AGAGTAACCC GGAAAAAGAG TCGCAGAAGA GTAACCCGGA AAAAGAGTCG 
CAGAAGAGTA ACCCGGAAAA AGAGTCGCAG AAGAGTAACC CGGAAAAAGA GTCGCAGAAG 
AGTAACCCGG AAAAAGAGTC GCAGAATGTT CTCCCAAGTC AGAAATCTCC TAATAAAGAC 
CAAGAACAAG CAATGGAAAC CGAATCATTC CAGTCATCAA AATCAGTAGA GACCCGTGAA 
ATAATCAAAC CTCATTCTGA CACAGAAAAG CCAGAGAATG CTGAAAAAAC TAAGCTTCAT 
GAAAGCAACA CAGAAAAAGA GTCAACTCAA AAACCATGCA TCTCTGAAGG AGACAAACTC 
TTAACCAGGG GAGAGTCTAT TGGTTCTAAA CCCTGTGAAG ATACGGATAA CCCTCAAGAC 
AAAAAACAAG AGAAGTCTGG CTCCTCCAAA TCGAGAACAG ATACTAATAA ATCATCTGTT 
TCTGATTCAA AAAAGTCAGA AACCAGAAAG AAAGTAGATG CTGAGGACAA ATGTGATGCA 
GCTGAAACGG CTAAAAGTGA CAAATCAGAA ATAGCAGAAG ACAAAGATAA AAAGAGTGAA 
AGTACAAAAA CATCAGATGA AGTTAAAGCT CCAGAAAAGC CCAATAAATC AGATAAAGAA 
CATGAAAAGC AACCAAATCC AATGGAAACT GATTCAGAGA TCCCTAAAAC TGCTGAGGAG 
ACAACAAAAG AAAGCGTGAA GGAAGCCAAT GCTACTGTAG ATAACTGCAC CAGCAAACAA 
TCAGAGCGAG ACGCGACTTC TGAAGAACGC GAGGCTTCCG GTGTTGTTCA AGAAAAGGGG 
AAAGGCATTA AAAAACCAGG ACGGCCTTCA AAGAAATCTA CTGAGAGTCA AAATAAGGAT 
AAAACTGTAG AAGAGGAAAA AGATGATGGA AAGCGACGGG GAAGAACCCC TCTGAAGTTG 
AAGAAACCAG CTCACAGGAG AAAGGCAGAG ATTCAGAGAG AAGAGCTGAA GGGAGATTCG 
GAGTCTGAGA CGACTGATGG ACGGTGTTTG AGAAGGTCTC CCAGAATCTG CAGACCAACC 
GCTAAAGCAG TGGAGATCCA GGATAAGAGG GCGGAGCGGA AACAGGCCAC GCCAGAGAAG 
GAGAAGGAGG AAGAAGAAGA AGAAGAGGAG GAGGAGCCTG TACAGAGGAA ACCGAGGAAA 
GCAGAGCCAG ATGGACAGAC AAAACCAAAG GTGGGGAGAC GTCGAAAACG ACAACCGTGG 
TCTCGCATGA GGCGTAAAAA GAAATCTTCG GAGGATGAGG AAGATGAAGA GGAGAGCGAT 
AGCGAAGAAG AGGAGACAGA GGAGGATGAC AGCGATGAAG ACTACAAGGT GGAGAAAAGC 
AAGAAGAGAA GGAGAAACCG AAACCGCGAA CGGCGCAGCT CTGACTCCTC CACCTCTTCA 
TCAGAAGACG ATACACCCAA TGACGACCCC TGCAAACACT GTGGCCTTCC CAATCATCCC 
GAACTGATCC TGCTGTGTGA TTCCTGTGAC AGCGGCTACC ACACCGCCTG CCTGCGTCCT 
CCGCTCATGA TCATTCCAGA CGGAGAGTGG TTCTGTCCTC CCTGCCAGCA CAAGCTACTT 
TGCGACAGAC TGGAAGAGCA GCTGCTGAAT TTGGATGCCG CCCTCAAAAA GAAAGAACGA 
GCAGAGCGAA GGAAAGAGCG ACTGGTGTAT GTTGGCATTA GTGTGGAGAA CATCATCACG 
CCCTCTGTTG AAGTGGAGGA AGAAAAGGAA GAGGTTTTGG TTAAGGATAA AGCGGAGACG 
AAGAAGAGCA AAGGCTGGGG ACGGAGATCA ACCAGAGCCA AGAAATACAT AAGCTACAGA 
TTCGATGAGT TTGATGAAGC GATCGAAGAG GCGATTGAGG AGGACATTAA AGAGGCAGAA 
GGTGGAGGAG CAGGACGAGG GAAGGACATG GCGAACATCA CAGGCCACAG AGGGAAGGAC 
ATGTCCACCA TCCTGCAGGA GGAAGGCAAA GAGAACGGCC GACCACAAAG AACTAGCGCA 
GCGCAGAAGA AGAAACGGCG GCGTCTCAAT GATCTGGACA GCGACAGCAC TGTAGATGAG 
GAGGAGAGCG AAGATGAGTT TCGCCTCAGC GACAGCAGTT CTGAGGAGGA GTTTGTGGCG 
TCTGAAAACG CTGAAAGTGA AGACAATGCG CCATCCTTAG AGGACAGCGA CTTTGACAGT 
GATGCTCAAG GTTCACGAGT GAATCGTGCG AACAGAAGGA AAACGAGCCG GAGTCGAATC 
CGGAGAAAAC AACCTCCGCG CAGGAGACGC AGACCCAGAG GATACTCTGA TGAAGAGGAG 
CTGGAGACTG ATGAAGAGGA GGAGGAAGAG GACGAGATGG TGACGGAGGG CTCTAGTGAG 
TTCAGTGACA GTGATCTGGA CGTCCGCTGT CGACGCTCGT ATCGCAGCCG AAAGCAGGAG 
GTGAATTACT GTGAAACTTC AGATTCAGAC GGCTCGCAAG CCAGCACCAA CAAAGACAAG 
AAAAAGAAGC GCAGACGACT GTCCAGCTCT GACAGCGAAG CCAGTTTCCA GTCAGCGGAA 
TCGGAAGAAG AAGAGCGAAA ACCCAAGAAG ATCAGAGCCG ACTCGTCTGA GGAGGAGGAA 
TCCAGGAAGC GACGCAGACG TTTATCCCTA AAGCGCCGAC GGGAGTCAGA GGACGATGAC 
GATGATGATG ATTCAGATGA ATCCGAGGAA GAGGAGCGTC CTGTCCGGAA GCGAGTAAAC 
CGAATCGACT CTGACGAAAG TGAGAGCGAA GAAGAGGAGA AGGAGGTGAA GAAGAGTCCG 
CAGGAGAAAG ACACAGAAGA GACGAGCGCT AGTAAAGGTC CCAGTCCAAT AAACGACAAC 
ACAGCGGAGC CTCCTTCTAC TAATGGACAG AGCCCTATAA AGGGTCTGGA GGGCGTCGCG 
CCACGGCCGG GAGCAGGAGC AACGCCTAAA AATGGCAACA CACCATCCAT AGCACCCAAT 
GGCCTGGAAG CCGGCGCACA GGAAGAAGAC GAAGACGATC TTTTGGGCGT CACAGACCTC 
GTAGACTATG TCTGCAACAG CGAACAGTTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rsf1b.1 ( XM_017358080.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213569.1 Danio rerio remodeling and spacing factor 1b, tandem duplicate 1 (rsf1b.1), transcript variant X3, mRNA.

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CloneID	ODa16391
Clone ID Related Accession (Same CDS sequence)	XM_017358080.1 , XM_017358080.2
Accession Version	XM_017358080.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	remodeling and spacing factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017358080.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCCT CGGAGGAAGC GGACGGTTCT TCCCCGGGAT TGTGCCCGAG CTTTGCCGTG 
ATCTGCTCGT TTCTGGAGCG GTACGGAGCT CTGCTGGACC TGCCGGAGGT GTCGTTCCCG 
GAGCTGGAGC GCAGCCTGCA GGACAGCAGC GCGGTACCCA AACTTCTTGT GGACCTTCAC 
GTGAAGTTAC TGAGGAAAAT TGGCAAGTCT GTGTCTGCAG AGAGATGGGA GAAATATCTC 
ATTAAGATTT GTCAGGAGTT TAACACCACA TGGGCATGGG AGTTGGAAAA GAAAGGTTAT 
CAAGAGTTGA CAGTGGAGTG TAAGACAGGA ATATTGAAGT ATTTGTGTGA ATGCCAGTTT 
GATGACAATG TCAAGTTTAA AAATGCTATC AATGAAGAAG ACCCGGATAA AATGCGACTT 
CAGCCTATTG GCCGAGACAA AGACGGCCTC ATGTACTGGT TCCAGCTGGA CCAAGACCAT 
AATGTAAGGA TCTATGTGGA AGAGCAGGAT GATTTGGATG GGTCGTCCTG GAAATGTATA 
GTAAGAAATA GAGATGACCT GGCTCAGATT CTTGGGTTGT TGAAGACTCA GATTGATCCA 
GCACTCCTGG TGAAGAAGGA ACAGGAGGAG GGCTCGTCTA ATAACAGTCC AAATCCCGAT 
GATGAGGAGA ACAAAAAGAA AGAGGGAGCT GAAGTGGAGA AGTCACAAGA TGATTTGTCC 
AAATCTGAAA ATAAGGAAAA CATATTAGAG AAAGAAAATA AAAAGGAAGA AGGTGCAGAT 
GCCACAGATG CTTTGTCGGA AAAGGGTTTA AAGGTAAAAG AGGAGCTGCA GAAACATTCA 
GAGACGGCAA ACGGATCTGA CGTTATTGCT GGATCAATCA AAGAAGAACC ACAGGATGAT 
GAGAAAGTCT TTTCCGCACC TTCAAAGAAA GCAGAACAGA CAGAGGAGGT GAAGAGAAAA 
ACACTAGAAG AGACCCAAAG AGCACTGAAA AATGACCATC AAGCTAAAAT CCCCCTGAAG 
AAGCGAGAGA TGAAGCTAAG TGAAGACTTT GACAGCAACA GCGCAGGACT TCGAAATGCT 
CCAGTAAAAG AGTCAAGGAC GTGTTTCGAT GATAGCATGT CGAGTGCTGA AAAGATCAAT 
GGCCATGTTC CTCACCTTCA GAAAGAAGCA ACAGATTCAG AAAGTCTCTC TCATTTTGAA 
TCCAATGAAA AAACAAAAGA CGCCCAGGTG GATAGAAAGA TGGACCCGAA TGAAACCAGA 
TTTCCTTCTG AAGAGAAGAT GGAAGTAGAG AAACCTAAGG AGAAAAATGA AGCTTCAACT 
AAACAGCACA AAACAACTCC TGGGGAAGAA AAACCAAGTG AACTATCAGA AAAGACACCT 
GATTCTGAAG TAGTAACTGA GAATATGGAA ACTATTGAAG ATATTAAGAC AACACCATGT 
GACGACACAG CAAATGAACT GCCTAAAGAG AAATCAATTA AAGATATTCC AAACAAAGAA 
TCAAAAGAAG ATTCATTGAA AGATGCACAA AGCAAAGAAT CAAAGGGAGA GTCATTGAAA 
GGAAGCAAAG AATCAAAAGG AGAATCATTA TTAAAAGATG CCCCAAGCAA AGAATCAAAA 
GAAGAATCAT TGAAAGATGC GCCGAGCAAA GAGTCAAAAG AATCATCATC AAAAGGTGCC 
CCAAGCAAAG AATCAAAAGG AGAATTATTT AAGGAAAGCA AAGAGTCGAA AAAAGAATCA 
TTAAAAGATA CCCCAAGTAA AGAATCAATA GGAGAATCAT TGAGAGATAC CCAAAGCAAA 
GAGTCAAAAG AATCATCATC AAAAGATGCC CCACGCAAAG AATCAAAAGG AGAATCATTG 
AAGGATGCCC CAAGCAAAGA GTCAAAAGAA GACTCAATGA AAGATACCCC AAGCAAAGAA 
TCAAATGAAT CATTATTGAA GGAAAGCAAA GAGTCAAAAG AAGAATCATT GAAAGATAAC 
CCAAGTAAAG AATCAAAAGA AGAAATATTG AAAGATACCC CAAGCAAAGA ATCAAAACAA 
GAATCATTGA AAGATACCCC AAGCAAAGAA TCAAAGGGAG AATCGTTGAA AGATGCCCCA 
AGCAAAGAAT CAAAAGAATC ATTATTGAAG GAAAGTAAAG AGTCAAAAGA AGAATCGTTG 
AAAGATCCCC CAGGCAAGGA GTCAGCAAAA TCAAAAGATG ACAAAACTAA AGCTCTTTCA 
AAAAAGGAAT CATTGGAAGA TATCTCAAAT GAAAAGTCAG CAAAATCTCT CAAAGATGAT 
GCAACAAAAG ATCCATCAAA AAAAGAATCA TTGCAAGATA TCTCAAACGA GTCAACAAAA 
TCTGTATCAA AAGACGACGC AGCACAAGAT CACTTTAACA AAGAATCATC AAAAGATCTC 
GCAAGCGAAG AGTCGGGGAA AGATCAGCCG GCAAAAGATC TTTCGAATGA AGAGTCTATA 
AAAAATCCTC CAAATCAAAA ACCAAGCAAA GATCTTGCAA AAAAAGAGTC ACAAAAGGTT 
ATCCCAGAAA GCGAATCACA AAAAGTTGTT TCAGAGAAAG ATTTGCAGAA GGGCATCCCA 
GAAAAAGGGT CACAGAAGAG TAACCCGGAA AAAGAGTCGC AGAAGAGTAA CCCGGAAAAA 
GAGTCGCAGA AGAGTAACCC GGAAAAAGAG TCGCAGAAGA GTAACCCGGA AAAAGAGTCG 
CAGAAGAGTA ACCCGGAAAA AGAGTCGCAG AAGAGTAACC CGGAAAAAGA GTCGCAGAAG 
AGTAACCCGG AAAAAGAGTC GCAGAATGTT CTCCCAAGTC AGAAATCTCC TAATAAAGAC 
CAAGAACAAG CAATGGAAAC CGAATCATTC CAGTCATCAA AATCAGTAGA GACCCGTGAA 
ATAATCAAAC CTCATTCTGA CACAGAAAAG CCAGAGAATG CTGAAAAAAC TAAGCTTCAT 
GAAAGCAACA CAGAAAAAGA GTCAACTCAA AAACCATGCA TCTCTGAAGG AGACAAACTC 
TTAACCAGGG GAGAGTCTAT TGGTTCTAAA CCCTGTGAAG ATACGGATAA CCCTCAAGAC 
AAAAAACAAG AGAAGTCTGG CTCCTCCAAA TCGAGAACAG ATACTAATAA ATCATCTGTT 
TCTGATTCAA AAAAGTCAGA AACCAGAAAG AAAGTAGATG CTGAGGACAA ATGTGATGCA 
GCTGAAACGG CTAAAAGTGA CAAATCAGAA ATAGCAGAAG ACAAAGATAA AAAGAGTGAA 
AGTACAAAAA CATCAGATGA AGTTAAAGCT CCAGAAAAGC CCAATAAATC AGATAAAGAA 
CATGAAAAGC AACCAAATCC AATGGAAACT GATTCAGAGA TCCCTAAAAC TGCTGAGGAG 
ACAACAAAAG AAAGCGTGAA GGAAGCCAAT GCTACTGTAG ATAACTGCAC CAGCAAACAA 
TCAGAGCGAG ACGCGACTTC TGAAGAACGC GAGGCTTCCG GTGTTGTTCA AGAAAAGGGG 
AAAGGCATTA AAAAACCAGG ACGGCCTTCA AAGAAATCTA CTGAGAGTCA AAATAAGGAT 
AAAACTGTAG AAGAGGAAAA AGATGATGGA AAGCGACGGG GAAGAACCCC TCTGAAGTTG 
AAGAAACCAG CTCACAGGAG AAAGGCAGAG ATTCAGAGAG AAGAGCTGAA GGGAGATTCG 
GAGTCTGAGA CGACTGATGG ACGGTGTTTG AGAAGGTCTC CCAGAATCTG CAGACCAACC 
GCTAAAGCAG TGGAGATCCA GGATAAGAGG GCGGAGCGGA AACAGGCCAC GCCAGAGAAG 
GAGAAGGAGG AAGAAGAAGA AGAAGAGGAG GAGGAGCCTG TACAGAGGAA ACCGAGGAAA 
GCAGAGCCAG ATGGACAGAC AAAACCAAAG GTGGGGAGAC GTCGAAAACG ACAACCGTGG 
TCTCGCATGA GGCGTAAAAA GAAATCTTCG GAGGATGAGG AAGATGAAGA GGAGAGCGAT 
AGCGAAGAAG AGGAGACAGA GGAGGATGAC AGCGATGAAG ACTACAAGGT GGAGAAAAGC 
AAGAAGAGAA GGAGAAACCG AAACCGCGAA CGGCGCAGCT CTGACTCCTC CACCTCTTCA 
TCAGAAGACG ATACACCCAA TGACGACCCC TGCAAACACT GTGGCCTTCC CAATCATCCC 
GAACTGATCC TGCTGTGTGA TTCCTGTGAC AGCGGCTACC ACACCGCCTG CCTGCGTCCT 
CCGCTCATGA TCATTCCAGA CGGAGAGTGG TTCTGTCCTC CCTGCCAGCA CAAGCTACTT 
TGCGACAGAC TGGAAGAGCA GCTGCTGAAT TTGGATGCCG CCCTCAAAAA GAAAGAACGA 
GCAGAGCGAA GGAAAGAGCG ACTGGTGTAT GTTGGCATTA GTGTGGAGAA CATCATCACG 
CCCTCTGTTG AAGTGGAGGA AGAAAAGGAA GAGGTTTTGG TTAAGGATAA AGCGGAGACG 
AAGAAGAGCA AAGGCTGGGG ACGGAGATCA ACCAGAGCCA AGAAATACAT AAGCTACAGA 
TTCGATGAGT TTGATGAAGC GATCGAAGAG GCGATTGAGG AGGACATTAA AGAGGCAGAA 
GGTGGAGGAG CAGGACGAGG GAAGGACATG GCGAACATCA CAGGCCACAG AGGGAAGGAC 
ATGTCCACCA TCCTGCAGGA GGAAGGCAAA GAGAACGGCC GACCACAAAG AACTAGCGCA 
GCGCAGAAGA AGAAACGGCG GCGTCTCAAT GATCTGGACA GCGACAGCAC TGTAGATGAG 
GAGGAGAGCG AAGATGAGTT TCGCCTCAGC GACAGCAGTT CTGAGGAGGA GTTTGTGGCG 
TCTGAAAACG CTGAAAGTGA AGACAATGCG CCATCCTTAG AGGACAGCGA CTTTGACAGT 
GATGCTCAAG GTTCACGAGT GAATCGTGCG AACAGAAGGA AAACGAGCCG GAGTCGAATC 
CGGAGAAAAC AACCTCCGCG CAGGAGACGC AGACCCAGAG GATACTCTGA TGAAGAGGAG 
CTGGAGACTG ATGAAGAGGA GGAGGAAGAG GACGAGATGG TGACGGAGGG CTCTAGTGAG 
TTCAGTGACA GTGATCTGGA CGTCCGCTGT CGACGCTCGT ATCGCAGCCG AAAGCAGGAG 
GTGAATTACT GTGAAACTTC AGATTCAGAC GGCTCGCAAG CCAGCACCAA CAAAGACAAG 
AAAAAGAAGC GCAGACGACT GTCCAGCTCT GACAGCGAAG CCAGTTTCCA GTCAGCGGAA 
TCGGAAGAAG AAGAGCGAAA ACCCAAGAAG ATCAGAGCCG ACTCGTCTGA GGAGGAGGAA 
TCCAGGAAGC GACGCAGACG TTTATCCCTA AAGCGCCGAC GGGAGTCAGA GGACGATGAC 
GATGATGATG ATTCAGATGA ATCCGAGGAA GAGGAGCGTC CTGTCCGGAA GCGAGTAAAC 
CGAATCGACT CTGACGAAAG TGAGAGCGAA GAAGAGGAGA AGGAGGTGAA GAAGAGTCCG 
CAGGAGAAAG ACACAGAAGA GACGAGCGCT AGTAAAGGTC CCAGTCCAAT AAACGACAAC 
ACAGCGGAGC CTCCTTCTAC TAATGGACAG AGCCCTATAA AGGGTCTGGA GGGCGTCGCG 
CCACGGCCGG GAGCAGGAGC AACGCCTAAA AATGGCAACA CACCATCCAT AGCACCCAAT 
GGCCTGGAAG CCGGCGCACA GGAAGAAGAC GAAGACGATC TTTTGGGCGT CACAGACCTC 
GTAGACTATG TCTGCAACAG CGAACAGTTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213569.1
CDS	96..5828
Translation	MSASEEADGSSPGLCPSFAVICSFLERYGALLDLPEVSFPELERSLQDSSAVPKLLVDLHVKLLRKIGKSVSAERWEKYLIKICQEFNTTWAWELEKKGYQELTVECKTGILKYLCECQFDDNVKFKNAINEEDPDKMRLQPIGRDKDGLMYWFQLDQDHNVRIYVEEQDDLDGSSWKCIVRNRDDLAQILGLLKTQIDPALLVKKEQEEGSSNNSPNPDDEENKKKEGAEVEKSQDDLSKSENKENILEKENKKEEGADATDALSEKGLKVKEELQKHSETANGSDVIAGSIKEEPQDDEKVFSAPSKKAEQTEEVKRKTLEETQRALKNDHQAKIPLKKREMKLSEDFDSNSAGLRNAPVKESRTCFDDSMSSAEKINGHVPHLQKEATDSESLSHFESNEKTKDAQVDRKMDPNETRFPSEEKMEVEKPKEKNEASTKQHKTTPGEEKPSELSEKTPDSEVVTENMETIEDIKTTPCDDTANELPKEKSIKDIPNKESKEDSLKDAQSKESKGESLKGSKESKGESLLKDAPSKESKEESLKDAPSKESKESSSKGAPSKESKGELFKESKESKKESLKDTPSKESIGESLRDTQSKESKESSSKDAPRKESKGESLKDAPSKESKEDSMKDTPSKESNESLLKESKESKEESLKDNPSKESKEEILKDTPSKESKQESLKDTPSKESKGESLKDAPSKESKESLLKESKESKEESLKDPPGKESAKSKDDKTKALSKKESLEDISNEKSAKSLKDDATKDPSKKESLQDISNESTKSVSKDDAAQDHFNKESSKDLASEESGKDQPAKDLSNEESIKNPPNQKPSKDLAKKESQKVIPESESQKVVSEKDLQKGIPEKGSQKSNPEKESQKSNPEKESQKSNPEKESQKSNPEKESQKSNPEKESQKSNPEKESQKSNPEKESQNVLPSQKSPNKDQEQAMETESFQSSKSVETREIIKPHSDTEKPENAEKTKLHESNTEKESTQKPCISEGDKLLTRGESIGSKPCEDTDNPQDKKQEKSGSSKSRTDTNKSSVSDSKKSETRKKVDAEDKCDAAETAKSDKSEIAEDKDKKSESTKTSDEVKAPEKPNKSDKEHEKQPNPMETDSEIPKTAEETTKESVKEANATVDNCTSKQSERDATSEEREASGVVQEKGKGIKKPGRPSKKSTESQNKDKTVEEEKDDGKRRGRTPLKLKKPAHRRKAEIQREELKGDSESETTDGRCLRRSPRICRPTAKAVEIQDKRAERKQATPEKEKEEEEEEEEEEPVQRKPRKAEPDGQTKPKVGRRRKRQPWSRMRRKKKSSEDEEDEEESDSEEEETEEDDSDEDYKVEKSKKRRRNRNRERRSSDSSTSSSEDDTPNDDPCKHCGLPNHPELILLCDSCDSGYHTACLRPPLMIIPDGEWFCPPCQHKLLCDRLEEQLLNLDAALKKKERAERRKERLVYVGISVENIITPSVEVEEEKEEVLVKDKAETKKSKGWGRRSTRAKKYISYRFDEFDEAIEEAIEEDIKEAEGGGAGRGKDMANITGHRGKDMSTILQEEGKENGRPQRTSAAQKKKRRRLNDLDSDSTVDEEESEDEFRLSDSSSEEEFVASENAESEDNAPSLEDSDFDSDAQGSRVNRANRRKTSRSRIRRKQPPRRRRRPRGYSDEEELETDEEEEEEDEMVTEGSSEFSDSDLDVRCRRSYRSRKQEVNYCETSDSDGSQASTNKDKKKKRRRLSSSDSEASFQSAESEEEERKPKKIRADSSEEEESRKRRRRLSLKRRRESEDDDDDDDSDESEEEERPVRKRVNRIDSDESESEEEEKEVKKSPQEKDTEETSASKGPSPINDNTAEPPSTNGQSPIKGLEGVAPRPGAGATPKNGNTPSIAPNGLEAGAQEEDEDDLLGVTDLVDYVCNSEQL

Target ORF information:

RefSeq Version	XM_017358080.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio remodeling and spacing factor 1b, tandem duplicate 1 (rsf1b.1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358080.2

ORF Insert Sequence:

ATGTCTGCCT CGGAGGAAGC GGACGGTTCT TCCCCGGGAT TGTGCCCGAG CTTTGCCGTG 
ATCTGCTCGT TTCTGGAGCG GTACGGAGCT CTGCTGGACC TGCCGGAGGT GTCGTTCCCG 
GAGCTGGAGC GCAGCCTGCA GGACAGCAGC GCGGTACCCA AACTTCTTGT GGACCTTCAC 
GTGAAGTTAC TGAGGAAAAT TGGCAAGTCT GTGTCTGCAG AGAGATGGGA GAAATATCTC 
ATTAAGATTT GTCAGGAGTT TAACACCACA TGGGCATGGG AGTTGGAAAA GAAAGGTTAT 
CAAGAGTTGA CAGTGGAGTG TAAGACAGGA ATATTGAAGT ATTTGTGTGA ATGCCAGTTT 
GATGACAATG TCAAGTTTAA AAATGCTATC AATGAAGAAG ACCCGGATAA AATGCGACTT 
CAGCCTATTG GCCGAGACAA AGACGGCCTC ATGTACTGGT TCCAGCTGGA CCAAGACCAT 
AATGTAAGGA TCTATGTGGA AGAGCAGGAT GATTTGGATG GGTCGTCCTG GAAATGTATA 
GTAAGAAATA GAGATGACCT GGCTCAGATT CTTGGGTTGT TGAAGACTCA GATTGATCCA 
GCACTCCTGG TGAAGAAGGA ACAGGAGGAG GGCTCGTCTA ATAACAGTCC AAATCCCGAT 
GATGAGGAGA ACAAAAAGAA AGAGGGAGCT GAAGTGGAGA AGTCACAAGA TGATTTGTCC 
AAATCTGAAA ATAAGGAAAA CATATTAGAG AAAGAAAATA AAAAGGAAGA AGGTGCAGAT 
GCCACAGATG CTTTGTCGGA AAAGGGTTTA AAGGTAAAAG AGGAGCTGCA GAAACATTCA 
GAGACGGCAA ACGGATCTGA CGTTATTGCT GGATCAATCA AAGAAGAACC ACAGGATGAT 
GAGAAAGTCT TTTCCGCACC TTCAAAGAAA GCAGAACAGA CAGAGGAGGT GAAGAGAAAA 
ACACTAGAAG AGACCCAAAG AGCACTGAAA AATGACCATC AAGCTAAAAT CCCCCTGAAG 
AAGCGAGAGA TGAAGCTAAG TGAAGACTTT GACAGCAACA GCGCAGGACT TCGAAATGCT 
CCAGTAAAAG AGTCAAGGAC GTGTTTCGAT GATAGCATGT CGAGTGCTGA AAAGATCAAT 
GGCCATGTTC CTCACCTTCA GAAAGAAGCA ACAGATTCAG AAAGTCTCTC TCATTTTGAA 
TCCAATGAAA AAACAAAAGA CGCCCAGGTG GATAGAAAGA TGGACCCGAA TGAAACCAGA 
TTTCCTTCTG AAGAGAAGAT GGAAGTAGAG AAACCTAAGG AGAAAAATGA AGCTTCAACT 
AAACAGCACA AAACAACTCC TGGGGAAGAA AAACCAAGTG AACTATCAGA AAAGACACCT 
GATTCTGAAG TAGTAACTGA GAATATGGAA ACTATTGAAG ATATTAAGAC AACACCATGT 
GACGACACAG CAAATGAACT GCCTAAAGAG AAATCAATTA AAGATATTCC AAACAAAGAA 
TCAAAAGAAG ATTCATTGAA AGATGCACAA AGCAAAGAAT CAAAGGGAGA GTCATTGAAA 
GGAAGCAAAG AATCAAAAGG AGAATCATTA TTAAAAGATG CCCCAAGCAA AGAATCAAAA 
GAAGAATCAT TGAAAGATGC GCCGAGCAAA GAGTCAAAAG AATCATCATC AAAAGGTGCC 
CCAAGCAAAG AATCAAAAGG AGAATTATTT AAGGAAAGCA AAGAGTCGAA AAAAGAATCA 
TTAAAAGATA CCCCAAGTAA AGAATCAATA GGAGAATCAT TGAGAGATAC CCAAAGCAAA 
GAGTCAAAAG AATCATCATC AAAAGATGCC CCACGCAAAG AATCAAAAGG AGAATCATTG 
AAGGATGCCC CAAGCAAAGA GTCAAAAGAA GACTCAATGA AAGATACCCC AAGCAAAGAA 
TCAAATGAAT CATTATTGAA GGAAAGCAAA GAGTCAAAAG AAGAATCATT GAAAGATAAC 
CCAAGTAAAG AATCAAAAGA AGAAATATTG AAAGATACCC CAAGCAAAGA ATCAAAACAA 
GAATCATTGA AAGATACCCC AAGCAAAGAA TCAAAGGGAG AATCGTTGAA AGATGCCCCA 
AGCAAAGAAT CAAAAGAATC ATTATTGAAG GAAAGTAAAG AGTCAAAAGA AGAATCGTTG 
AAAGATCCCC CAGGCAAGGA GTCAGCAAAA TCAAAAGATG ACAAAACTAA AGCTCTTTCA 
AAAAAGGAAT CATTGGAAGA TATCTCAAAT GAAAAGTCAG CAAAATCTCT CAAAGATGAT 
GCAACAAAAG ATCCATCAAA AAAAGAATCA TTGCAAGATA TCTCAAACGA GTCAACAAAA 
TCTGTATCAA AAGACGACGC AGCACAAGAT CACTTTAACA AAGAATCATC AAAAGATCTC 
GCAAGCGAAG AGTCGGGGAA AGATCAGCCG GCAAAAGATC TTTCGAATGA AGAGTCTATA 
AAAAATCCTC CAAATCAAAA ACCAAGCAAA GATCTTGCAA AAAAAGAGTC ACAAAAGGTT 
ATCCCAGAAA GCGAATCACA AAAAGTTGTT TCAGAGAAAG ATTTGCAGAA GGGCATCCCA 
GAAAAAGGGT CACAGAAGAG TAACCCGGAA AAAGAGTCGC AGAAGAGTAA CCCGGAAAAA 
GAGTCGCAGA AGAGTAACCC GGAAAAAGAG TCGCAGAAGA GTAACCCGGA AAAAGAGTCG 
CAGAAGAGTA ACCCGGAAAA AGAGTCGCAG AAGAGTAACC CGGAAAAAGA GTCGCAGAAG 
AGTAACCCGG AAAAAGAGTC GCAGAATGTT CTCCCAAGTC AGAAATCTCC TAATAAAGAC 
CAAGAACAAG CAATGGAAAC CGAATCATTC CAGTCATCAA AATCAGTAGA GACCCGTGAA 
ATAATCAAAC CTCATTCTGA CACAGAAAAG CCAGAGAATG CTGAAAAAAC TAAGCTTCAT 
GAAAGCAACA CAGAAAAAGA GTCAACTCAA AAACCATGCA TCTCTGAAGG AGACAAACTC 
TTAACCAGGG GAGAGTCTAT TGGTTCTAAA CCCTGTGAAG ATACGGATAA CCCTCAAGAC 
AAAAAACAAG AGAAGTCTGG CTCCTCCAAA TCGAGAACAG ATACTAATAA ATCATCTGTT 
TCTGATTCAA AAAAGTCAGA AACCAGAAAG AAAGTAGATG CTGAGGACAA ATGTGATGCA 
GCTGAAACGG CTAAAAGTGA CAAATCAGAA ATAGCAGAAG ACAAAGATAA AAAGAGTGAA 
AGTACAAAAA CATCAGATGA AGTTAAAGCT CCAGAAAAGC CCAATAAATC AGATAAAGAA 
CATGAAAAGC AACCAAATCC AATGGAAACT GATTCAGAGA TCCCTAAAAC TGCTGAGGAG 
ACAACAAAAG AAAGCGTGAA GGAAGCCAAT GCTACTGTAG ATAACTGCAC CAGCAAACAA 
TCAGAGCGAG ACGCGACTTC TGAAGAACGC GAGGCTTCCG GTGTTGTTCA AGAAAAGGGG 
AAAGGCATTA AAAAACCAGG ACGGCCTTCA AAGAAATCTA CTGAGAGTCA AAATAAGGAT 
AAAACTGTAG AAGAGGAAAA AGATGATGGA AAGCGACGGG GAAGAACCCC TCTGAAGTTG 
AAGAAACCAG CTCACAGGAG AAAGGCAGAG ATTCAGAGAG AAGAGCTGAA GGGAGATTCG 
GAGTCTGAGA CGACTGATGG ACGGTGTTTG AGAAGGTCTC CCAGAATCTG CAGACCAACC 
GCTAAAGCAG TGGAGATCCA GGATAAGAGG GCGGAGCGGA AACAGGCCAC GCCAGAGAAG 
GAGAAGGAGG AAGAAGAAGA AGAAGAGGAG GAGGAGCCTG TACAGAGGAA ACCGAGGAAA 
GCAGAGCCAG ATGGACAGAC AAAACCAAAG GTGGGGAGAC GTCGAAAACG ACAACCGTGG 
TCTCGCATGA GGCGTAAAAA GAAATCTTCG GAGGATGAGG AAGATGAAGA GGAGAGCGAT 
AGCGAAGAAG AGGAGACAGA GGAGGATGAC AGCGATGAAG ACTACAAGGT GGAGAAAAGC 
AAGAAGAGAA GGAGAAACCG AAACCGCGAA CGGCGCAGCT CTGACTCCTC CACCTCTTCA 
TCAGAAGACG ATACACCCAA TGACGACCCC TGCAAACACT GTGGCCTTCC CAATCATCCC 
GAACTGATCC TGCTGTGTGA TTCCTGTGAC AGCGGCTACC ACACCGCCTG CCTGCGTCCT 
CCGCTCATGA TCATTCCAGA CGGAGAGTGG TTCTGTCCTC CCTGCCAGCA CAAGCTACTT 
TGCGACAGAC TGGAAGAGCA GCTGCTGAAT TTGGATGCCG CCCTCAAAAA GAAAGAACGA 
GCAGAGCGAA GGAAAGAGCG ACTGGTGTAT GTTGGCATTA GTGTGGAGAA CATCATCACG 
CCCTCTGTTG AAGTGGAGGA AGAAAAGGAA GAGGTTTTGG TTAAGGATAA AGCGGAGACG 
AAGAAGAGCA AAGGCTGGGG ACGGAGATCA ACCAGAGCCA AGAAATACAT AAGCTACAGA 
TTCGATGAGT TTGATGAAGC GATCGAAGAG GCGATTGAGG AGGACATTAA AGAGGCAGAA 
GGTGGAGGAG CAGGACGAGG GAAGGACATG GCGAACATCA CAGGCCACAG AGGGAAGGAC 
ATGTCCACCA TCCTGCAGGA GGAAGGCAAA GAGAACGGCC GACCACAAAG AACTAGCGCA 
GCGCAGAAGA AGAAACGGCG GCGTCTCAAT GATCTGGACA GCGACAGCAC TGTAGATGAG 
GAGGAGAGCG AAGATGAGTT TCGCCTCAGC GACAGCAGTT CTGAGGAGGA GTTTGTGGCG 
TCTGAAAACG CTGAAAGTGA AGACAATGCG CCATCCTTAG AGGACAGCGA CTTTGACAGT 
GATGCTCAAG GTTCACGAGT GAATCGTGCG AACAGAAGGA AAACGAGCCG GAGTCGAATC 
CGGAGAAAAC AACCTCCGCG CAGGAGACGC AGACCCAGAG GATACTCTGA TGAAGAGGAG 
CTGGAGACTG ATGAAGAGGA GGAGGAAGAG GACGAGATGG TGACGGAGGG CTCTAGTGAG 
TTCAGTGACA GTGATCTGGA CGTCCGCTGT CGACGCTCGT ATCGCAGCCG AAAGCAGGAG 
GTGAATTACT GTGAAACTTC AGATTCAGAC GGCTCGCAAG CCAGCACCAA CAAAGACAAG 
AAAAAGAAGC GCAGACGACT GTCCAGCTCT GACAGCGAAG CCAGTTTCCA GTCAGCGGAA 
TCGGAAGAAG AAGAGCGAAA ACCCAAGAAG ATCAGAGCCG ACTCGTCTGA GGAGGAGGAA 
TCCAGGAAGC GACGCAGACG TTTATCCCTA AAGCGCCGAC GGGAGTCAGA GGACGATGAC 
GATGATGATG ATTCAGATGA ATCCGAGGAA GAGGAGCGTC CTGTCCGGAA GCGAGTAAAC 
CGAATCGACT CTGACGAAAG TGAGAGCGAA GAAGAGGAGA AGGAGGTGAA GAAGAGTCCG 
CAGGAGAAAG ACACAGAAGA GACGAGCGCT AGTAAAGGTC CCAGTCCAAT AAACGACAAC 
ACAGCGGAGC CTCCTTCTAC TAATGGACAG AGCCCTATAA AGGGTCTGGA GGGCGTCGCG 
CCACGGCCGG GAGCAGGAGC AACGCCTAAA AATGGCAACA CACCATCCAT AGCACCCAAT 
GGCCTGGAAG CCGGCGCACA GGAAGAAGAC GAAGACGATC TTTTGGGCGT CACAGACCTC 
GTAGACTATG TCTGCAACAG CGAACAGTTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rsf1b.1 ( XM_017358079.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213568.1 Danio rerio remodeling and spacing factor 1b, tandem duplicate 1 (rsf1b.1), transcript variant X2, mRNA.

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CloneID	ODa39952
Clone ID Related Accession (Same CDS sequence)	XM_017358079.1 , XM_017358079.2
Accession Version	XM_017358079.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	remodeling and spacing factor 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCCT CGGAGGAAGC GGACGGTTCT TCCCCGGGAT TGTGCCCGAG CTTTGCCGTG 
ATCTGCTCGT TTCTGGAGCG GTACGGAGCT CTGCTGGACC TGCCGGAGGT GTCGTTCCCG 
GAGCTGGAGC GCAGCCTGCA GGACAGCAGC GCGGTACCCA AACTTCTTGT GGACCTTCAC 
GTGAAGTTAC TGAGGAAAAT TGGCAAGTCT GTGTCTGCAG AGAGATGGGA GAAATATCTC 
ATTAAGATTT GTCAGGAGTT TAACACCACA TGGGCATGGG AGTTGGAAAA GAAAGGTTAT 
CAAGAGTTGA CAGTGGAGTG TAAGACAGGA ATATTGAAGT ATTTGTGTGA ATGCCAGTTT 
GATGACAATG TCAAGTTTAA AAATGCTATC AATGAAGAAG ACCCGGATAA AATGCGACTT 
CAGCCTATTG GCCGAGACAA AGACGGCCTC ATGTACTGGT TCCAGCTGGA CCAAGACCAT 
AATGTAAGGA TCTATGTGGA AGAGCAGGAT GATTTGGATG GGTCGTCCTG GAAATGTATA 
GTAAGAAATA GAGATGACCT GGCTCAGATT CTTGGGTTGT TGAAGACTCA GATTGATCCA 
GCACTCCTGG TGAAGAAGGA ACAGGAGGAG GGCTCGTCTA ATAACAGTCC AAATCCCGAT 
GATGAGGAGA ACAAAAAGAA AGAGGGAGCT GAAGTGGAGA AGTCACAAGA TGATTTGTCC 
AAATCTGAAA ATAAGGAAAA CATATTAGAG AAAGAAAATA AAAAGGAAGA AGGTGCAGAT 
GCCACAGATG CTTTGTCGGA AAAGGGTTTA AAGGTAAAAG AGGAGCTGCA GAAACATTCA 
GAGACGGCAA ACGGATCTGA CGTTATTGCT GGATCAATCA AAGAAGAACC ACAGGATGAT 
GAGAAAGTCT TTTCCGCACC TTCAAAGAAA GCAGAACAGA CAGAGGAGGT GAAGAGAAAA 
ACACTAGAAG AGACCCAAAG AGCACTGAAA AATGACCATC AAGCTAAAAT CCCCCTGAAG 
AAGCGAGAGA TGAAGCTAAG TGAAGACTTT GACAGCAACA GCGCAGGACT TCGAAATGCT 
CCAGTAAAAG AGTCAAGGAC GTGTTTCGAT GATAGCATGT CGAGTGCTGA AAAGATCAAT 
GGCCATGTTC CTCACCTTCA GAAAGAAGCA ACAGATTCAG AAAGTCTCTC TCATTTTGAA 
TCCAATGAAA AAACAAAAGA CGCCCAGGTG GATAGAAAGA TGGACCCGAA TGAAACCAGA 
TTTCCTTCTG AAGAGAAGAT GGAAGTAGAG AAACCTAAGG AGAAAAATGA AGCTTCAACT 
AAACAGCACA AAACAACTCC TGGGGAAGAA AAACCAAGTG AACTATCAGA AAAGACACCT 
GATTCTGAAG TAGTAACTGA GAATATGGAA ACTATTGAAG ATATTAAGAC AACACCATGT 
GACGACACAG CAAATGAACT GCCTAAAGAG AAATCAATTA AAGATATTCC AAACAAAGAA 
TCAAAAGAAG ATTCATTGAA AGATGCACAA AGCAAAGAAT CAAAGGGAGA GTCATTGAAA 
GGAAGCAAAG AATCAAAAGG AGAATCATTA TTAAAAGATG CCCCAAGCAA AGAATCAAAA 
GAAGAATCAT TGAAAGATGC GCCGAGCAAA GAGTCAAAAG AATCATCATC AAAAGGTGCC 
CCAAGCAAAG AATCAAAAGG AGAATTATTT AAGGAAAGCA AAGAGTCGAA AAAAGAATCA 
TTAAAAGATA CCCCAAGTAA AGAATCAATA GGAGAATCAT TGAGAGATAC CCAAAGCAAA 
GAGTCAAAAG AATCATCATC AAAAGATGCC CCACGCAAAG AATCAAAAGG AGAATCATTG 
AAGGATGCCC CAAGCAAAGA GTCAAAAGAA GACTCAATGA AAGATACCCC AAGCAAAGAA 
TCAAATGAAT CATTATTGAA GGAAAGCAAA GAGTCAAAAG AAGAATCATT GAAAGATAAC 
CCAAGTAAAG AATCAAAAGA AGAAATATTG AAAGATACCC CAAGCAAAGA ATCAAAACAA 
GAATCATTGA AAGATACCCC AAGCAAAGAA TCAAAGGGAG AATCGTTGAA AGATGCCCCA 
AGCAAAGAAT CAAAAGAATC ATTATTGAAG GAAAGTAAAG AGTCAAAAGA AGAATCGTTG 
AAAGATCCCC CAGGCAAGGA GTCAGCAAAA TCAAAAGATG ACAAAACTAA AGCTCTTTCA 
AAAAAGGAAT CATTGGAAGA TATCTCAAAT GAAAAGTCAG CAAAATCTCT CAAAGATGAT 
GCAACAAAAG ATCCATCAAA AAAAGAATCA TTGCAAGATA TCTCAAACGA GTCAACAAAA 
TCTGTATCAA AAGACGACGC AGCACAAGAT CACTTTAACA AAGAATCATC AAAAGATCTC 
GCAAGCGAAG AGTCGGGGAA AGATCAGCCG GCAAAAGATC TTTCGAATGA AGAGTCTATA 
AAAAATCCTC CAAATCAAAA ACCAAGCAAA GATCTTGCAA AAAAAGAGTC ACAAAAGGTT 
ATCCCAGAAA GCGAATCACA AAAAGTTGTT TCAGAGAAAG ATTTGCAGAA GGGCATCCCA 
GAAAAAGGGT CACAGAAGAG TAACCCGGAA AAAGAGTCGC AGAAGAGTAA CCCGGAAAAA 
GAGTCGCAGA AGAGTAACCC GGAAAAAGAG TCGCAGAAGA GTAACCCGGA AAAAGAGTCG 
CAGAAGAGTA ACCCGGAAAA AGAGTCGCAG AAGAGTAACC CGGAAAAAGA GTCGCAGAAG 
AGTAACCCGG AAAAAGAGTC GCAGAATGTT CTCCCAAGTC AGAAATCTCC TAATAAAGAC 
CAAGAACAAG CAATGGAAAC CGAATCATTC CAGTCATCAA AATCAGTAGA GACCCGTGAA 
ATAATCAAAC CTCATTCTGA CACAGAAAAG CCAGAGAATG CTGAAAAAAC TAAGCTTCAT 
GAAAGCAACA CAGAAAAAGA GTCAACTCAA AAACCATGCA TCTCTGAAGG AGACAAACTC 
TTAACCAGGG GAGAGTCTAT TGGTTCTAAA CCCTGTGAAG ATACGGATAA CCCTCAAGAC 
AAAAAACAAG AGAAGTCTGG CTCCTCCAAA TCGAGAACAG ATACTAATAA ATCATCTGTT 
TCTGATTCAA AAAAGTCAGA AACCAGAAAG AAAGTAGATG CTGAGGACAA ATGTGATGCA 
GCTGAAACGG CTAAAAGTGA CAAATCAGAA ATAGCAGAAG ACAAAGATAA AAAGAGTGAA 
AGTACAAAAA CATCAGATGA AGTTAAAGCT CCAGAAAAGC CCAATAAATC AGATAAAGAA 
CATGAAAAGC AACCAAATCC AATGGAAACT GATTCAGAGA TCCCTAAAAC TGCTGAGGAG 
ACAACAAAAG AAAGCGTGAA GGAAGCCAAT GCTACTGTAG ATAACTGCAC CAGCAAACAA 
TCAGAGCGAG ACGCGACTTC TGAAGAACGC GAGGCTTCCG GTGTTGTTCA AGAAAAGGGG 
AAAGGCATTA AAAAACCAGG ACGGCCTTCA AAGAAATCTA CTGAGAGTCA AAATAAGGAT 
AAAACTGTAG AAGAGGAAAA AGATGATGGA AAGCGACGGG GAAGAACCCC TCTGAAGTTG 
AAGAAACCAG CTCACAGGAG AAAGGCAGAG ATTCAGAGAG AAGAGCTGAA GGGAGATTCG 
GAGTCTGAGA CGACTGATGG ACGGTGTTTG AGAAGGTCTC CCAGAATCTG CAGACCAACC 
GCTAAAGCAG TGGAGATCCA GGATAAGAGG GCGGAGCGGA AACAGGCCAC GCCAGAGAAG 
GAGAAGGAGG AAGAAGAAGA AGAAGAGGAG GAGGAGCCTG TACAGAGGAA ACCGAGGAAA 
GCAGAGCCAG ATGGACAGAC AAAACCAAAG CAGGTGGGGA GACGTCGAAA ACGACAACCG 
TGGTCTCGCA TGAGGCGTAA AAAGAAATCT TCGGAGGATG AGGAAGATGA AGAGGAGAGC 
GATAGCGAAG AAGAGGAGAC AGAGGAGGAT GACAGCGATG AAGACTACAA GGTGGAGAAA 
AGCAAGAAGA GAAGGAGAAA CCGAAACCGC GAACGGCGCA GCTCTGACTC CTCCACCTCT 
TCATCAGAAG ACGATACACC CAATGACGAC CCCTGCAAAC ACTGTGGCCT TCCCAATCAT 
CCCGAACTGA TCCTGCTGTG TGATTCCTGT GACAGCGGCT ACCACACCGC CTGCCTGCGT 
CCTCCGCTCA TGATCATTCC AGACGGAGAG TGGTTCTGTC CTCCCTGCCA GCACAAGCTA 
CTTTGCGACA GACTGGAAGA GCAGCTGCTG AATTTGGATG CCGCCCTCAA AAAGAAAGAA 
CGAGCAGAGC GAAGGAAAGA GCGACTGGTG TATGTTGGCA TTAGTGTGGA GAACATCATC 
ACGCCCTCTG TTGAAGTGGA GGAAGAAAAG GAAGAGGTTT TGGTTAAGGA TAAAGCGGAG 
ACGAAGAAGA GCAAAGGCTG GGGACGGAGA TCAACCAGAG CCAAGAAATA CATAAGCTAC 
AGATTCGATG AGTTTGATGA AGCGATCGAA GAGGCGATTG AGGAGGACAT TAAAGAGGCA 
GAAGGTGGAG GAGCAGGACG AGGGAAGGAC ATGGCGAACA TCACAGGCCA CAGAGGGAAG 
GACATGTCCA CCATCCTGCA GGAGGAAGGC AAAGAGAACG GCCGACCACA AAGAACTAGC 
GCAGCGCAGA AGAAGAAACG GCGGCGTCTC AATGATCTGG ACAGCGACAG CACTGTAGAT 
GAGGAGGAGA GCGAAGATGA GTTTCGCCTC AGCGACAGTT CTGAGGAGGA GTTTGTGGCG 
TCTGAAAACG CTGAAAGTGA AGACAATGCG CCATCCTTAG AGGACAGCGA CTTTGACAGT 
GATGCTCAAG GTTCACGAGT GAATCGTGCG AACAGAAGGA AAACGAGCCG GAGTCGAATC 
CGGAGAAAAC AACCTCCGCG CAGGAGACGC AGACCCAGAG GATACTCTGA TGAAGAGGAG 
CTGGAGACTG ATGAAGAGGA GGAGGAAGAG GACGAGATGG TGACGGAGGG CTCTAGTGAG 
TTCAGTGACA GTGATCTGGA CGTCCGCTGT CGACGCTCGT ATCGCAGCCG AAAGCAGGAG 
GTGAATTACT GTGAAACTTC AGATTCAGAC GGCTCGCAAG CCAGCACCAA CAAAGACAAG 
AAAAAGAAGC GCAGACGACT GTCCAGCTCT GACAGCGAAG CCAGTTTCCA GTCAGCGGAA 
TCGGAAGAAG AAGAGCGAAA ACCCAAGAAG ATCAGAGCCG ACTCGTCTGA GGAGGAGGAA 
TCCAGGAAGC GACGCAGACG TTTATCCCTA AAGCGCCGAC GGGAGTCAGA GGACGATGAC 
GATGATGATG ATTCAGATGA ATCCGAGGAA GAGGAGCGTC CTGTCCGGAA GCGAGTAAAC 
CGAATCGACT CTGACGAAAG TGAGAGCGAA GAAGAGGAGA AGGAGGTGAA GAAGAGTCCG 
CAGGAGAAAG ACACAGAAGA GACGAGCGCT AGTAAAGGTC CCAGTCCAAT AAACGACAAC 
ACAGCGGAGC CTCCTTCTAC TAATGGACAG AGCCCTATAA AGGGTCTGGA GGGCGTCGCG 
CCACGGCCGG GAGCAGGAGC AACGCCTAAA AATGGCAACA CACCATCCAT AGCACCCAAT 
GGCCTGGAAG CCGGCGCACA GGAAGAAGAC GAAGACGATC TTTTGGGCGT CACAGACCTC 
GTAGACTATG TCTGCAACAG CGAACAGTTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213568.1
CDS	52..5784
Translation	MSASEEADGSSPGLCPSFAVICSFLERYGALLDLPEVSFPELERSLQDSSAVPKLLVDLHVKLLRKIGKSVSAERWEKYLIKICQEFNTTWAWELEKKGYQELTVECKTGILKYLCECQFDDNVKFKNAINEEDPDKMRLQPIGRDKDGLMYWFQLDQDHNVRIYVEEQDDLDGSSWKCIVRNRDDLAQILGLLKTQIDPALLVKKEQEEGSSNNSPNPDDEENKKKEGAEVEKSQDDLSKSENKENILEKENKKEEGADATDALSEKGLKVKEELQKHSETANGSDVIAGSIKEEPQDDEKVFSAPSKKAEQTEEVKRKTLEETQRALKNDHQAKIPLKKREMKLSEDFDSNSAGLRNAPVKESRTCFDDSMSSAEKINGHVPHLQKEATDSESLSHFESNEKTKDAQVDRKMDPNETRFPSEEKMEVEKPKEKNEASTKQHKTTPGEEKPSELSEKTPDSEVVTENMETIEDIKTTPCDDTANELPKEKSIKDIPNKESKEDSLKDAQSKESKGESLKGSKESKGESLLKDAPSKESKEESLKDAPSKESKESSSKGAPSKESKGELFKESKESKKESLKDTPSKESIGESLRDTQSKESKESSSKDAPRKESKGESLKDAPSKESKEDSMKDTPSKESNESLLKESKESKEESLKDNPSKESKEEILKDTPSKESKQESLKDTPSKESKGESLKDAPSKESKESLLKESKESKEESLKDPPGKESAKSKDDKTKALSKKESLEDISNEKSAKSLKDDATKDPSKKESLQDISNESTKSVSKDDAAQDHFNKESSKDLASEESGKDQPAKDLSNEESIKNPPNQKPSKDLAKKESQKVIPESESQKVVSEKDLQKGIPEKGSQKSNPEKESQKSNPEKESQKSNPEKESQKSNPEKESQKSNPEKESQKSNPEKESQKSNPEKESQNVLPSQKSPNKDQEQAMETESFQSSKSVETREIIKPHSDTEKPENAEKTKLHESNTEKESTQKPCISEGDKLLTRGESIGSKPCEDTDNPQDKKQEKSGSSKSRTDTNKSSVSDSKKSETRKKVDAEDKCDAAETAKSDKSEIAEDKDKKSESTKTSDEVKAPEKPNKSDKEHEKQPNPMETDSEIPKTAEETTKESVKEANATVDNCTSKQSERDATSEEREASGVVQEKGKGIKKPGRPSKKSTESQNKDKTVEEEKDDGKRRGRTPLKLKKPAHRRKAEIQREELKGDSESETTDGRCLRRSPRICRPTAKAVEIQDKRAERKQATPEKEKEEEEEEEEEEPVQRKPRKAEPDGQTKPKQVGRRRKRQPWSRMRRKKKSSEDEEDEEESDSEEEETEEDDSDEDYKVEKSKKRRRNRNRERRSSDSSTSSSEDDTPNDDPCKHCGLPNHPELILLCDSCDSGYHTACLRPPLMIIPDGEWFCPPCQHKLLCDRLEEQLLNLDAALKKKERAERRKERLVYVGISVENIITPSVEVEEEKEEVLVKDKAETKKSKGWGRRSTRAKKYISYRFDEFDEAIEEAIEEDIKEAEGGGAGRGKDMANITGHRGKDMSTILQEEGKENGRPQRTSAAQKKKRRRLNDLDSDSTVDEEESEDEFRLSDSSEEEFVASENAESEDNAPSLEDSDFDSDAQGSRVNRANRRKTSRSRIRRKQPPRRRRRPRGYSDEEELETDEEEEEEDEMVTEGSSEFSDSDLDVRCRRSYRSRKQEVNYCETSDSDGSQASTNKDKKKKRRRLSSSDSEASFQSAESEEEERKPKKIRADSSEEEESRKRRRRLSLKRRRESEDDDDDDDSDESEEEERPVRKRVNRIDSDESESEEEEKEVKKSPQEKDTEETSASKGPSPINDNTAEPPSTNGQSPIKGLEGVAPRPGAGATPKNGNTPSIAPNGLEAGAQEEDEDDLLGVTDLVDYVCNSEQL

Target ORF information:

RefSeq Version	XM_017358079.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio remodeling and spacing factor 1b, tandem duplicate 1 (rsf1b.1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358079.1

ORF Insert Sequence:

ATGTCTGCCT CGGAGGAAGC GGACGGTTCT TCCCCGGGAT TGTGCCCGAG CTTTGCCGTG 
ATCTGCTCGT TTCTGGAGCG GTACGGAGCT CTGCTGGACC TGCCGGAGGT GTCGTTCCCG 
GAGCTGGAGC GCAGCCTGCA GGACAGCAGC GCGGTACCCA AACTTCTTGT GGACCTTCAC 
GTGAAGTTAC TGAGGAAAAT TGGCAAGTCT GTGTCTGCAG AGAGATGGGA GAAATATCTC 
ATTAAGATTT GTCAGGAGTT TAACACCACA TGGGCATGGG AGTTGGAAAA GAAAGGTTAT 
CAAGAGTTGA CAGTGGAGTG TAAGACAGGA ATATTGAAGT ATTTGTGTGA ATGCCAGTTT 
GATGACAATG TCAAGTTTAA AAATGCTATC AATGAAGAAG ACCCGGATAA AATGCGACTT 
CAGCCTATTG GCCGAGACAA AGACGGCCTC ATGTACTGGT TCCAGCTGGA CCAAGACCAT 
AATGTAAGGA TCTATGTGGA AGAGCAGGAT GATTTGGATG GGTCGTCCTG GAAATGTATA 
GTAAGAAATA GAGATGACCT GGCTCAGATT CTTGGGTTGT TGAAGACTCA GATTGATCCA 
GCACTCCTGG TGAAGAAGGA ACAGGAGGAG GGCTCGTCTA ATAACAGTCC AAATCCCGAT 
GATGAGGAGA ACAAAAAGAA AGAGGGAGCT GAAGTGGAGA AGTCACAAGA TGATTTGTCC 
AAATCTGAAA ATAAGGAAAA CATATTAGAG AAAGAAAATA AAAAGGAAGA AGGTGCAGAT 
GCCACAGATG CTTTGTCGGA AAAGGGTTTA AAGGTAAAAG AGGAGCTGCA GAAACATTCA 
GAGACGGCAA ACGGATCTGA CGTTATTGCT GGATCAATCA AAGAAGAACC ACAGGATGAT 
GAGAAAGTCT TTTCCGCACC TTCAAAGAAA GCAGAACAGA CAGAGGAGGT GAAGAGAAAA 
ACACTAGAAG AGACCCAAAG AGCACTGAAA AATGACCATC AAGCTAAAAT CCCCCTGAAG 
AAGCGAGAGA TGAAGCTAAG TGAAGACTTT GACAGCAACA GCGCAGGACT TCGAAATGCT 
CCAGTAAAAG AGTCAAGGAC GTGTTTCGAT GATAGCATGT CGAGTGCTGA AAAGATCAAT 
GGCCATGTTC CTCACCTTCA GAAAGAAGCA ACAGATTCAG AAAGTCTCTC TCATTTTGAA 
TCCAATGAAA AAACAAAAGA CGCCCAGGTG GATAGAAAGA TGGACCCGAA TGAAACCAGA 
TTTCCTTCTG AAGAGAAGAT GGAAGTAGAG AAACCTAAGG AGAAAAATGA AGCTTCAACT 
AAACAGCACA AAACAACTCC TGGGGAAGAA AAACCAAGTG AACTATCAGA AAAGACACCT 
GATTCTGAAG TAGTAACTGA GAATATGGAA ACTATTGAAG ATATTAAGAC AACACCATGT 
GACGACACAG CAAATGAACT GCCTAAAGAG AAATCAATTA AAGATATTCC AAACAAAGAA 
TCAAAAGAAG ATTCATTGAA AGATGCACAA AGCAAAGAAT CAAAGGGAGA GTCATTGAAA 
GGAAGCAAAG AATCAAAAGG AGAATCATTA TTAAAAGATG CCCCAAGCAA AGAATCAAAA 
GAAGAATCAT TGAAAGATGC GCCGAGCAAA GAGTCAAAAG AATCATCATC AAAAGGTGCC 
CCAAGCAAAG AATCAAAAGG AGAATTATTT AAGGAAAGCA AAGAGTCGAA AAAAGAATCA 
TTAAAAGATA CCCCAAGTAA AGAATCAATA GGAGAATCAT TGAGAGATAC CCAAAGCAAA 
GAGTCAAAAG AATCATCATC AAAAGATGCC CCACGCAAAG AATCAAAAGG AGAATCATTG 
AAGGATGCCC CAAGCAAAGA GTCAAAAGAA GACTCAATGA AAGATACCCC AAGCAAAGAA 
TCAAATGAAT CATTATTGAA GGAAAGCAAA GAGTCAAAAG AAGAATCATT GAAAGATAAC 
CCAAGTAAAG AATCAAAAGA AGAAATATTG AAAGATACCC CAAGCAAAGA ATCAAAACAA 
GAATCATTGA AAGATACCCC AAGCAAAGAA TCAAAGGGAG AATCGTTGAA AGATGCCCCA 
AGCAAAGAAT CAAAAGAATC ATTATTGAAG GAAAGTAAAG AGTCAAAAGA AGAATCGTTG 
AAAGATCCCC CAGGCAAGGA GTCAGCAAAA TCAAAAGATG ACAAAACTAA AGCTCTTTCA 
AAAAAGGAAT CATTGGAAGA TATCTCAAAT GAAAAGTCAG CAAAATCTCT CAAAGATGAT 
GCAACAAAAG ATCCATCAAA AAAAGAATCA TTGCAAGATA TCTCAAACGA GTCAACAAAA 
TCTGTATCAA AAGACGACGC AGCACAAGAT CACTTTAACA AAGAATCATC AAAAGATCTC 
GCAAGCGAAG AGTCGGGGAA AGATCAGCCG GCAAAAGATC TTTCGAATGA AGAGTCTATA 
AAAAATCCTC CAAATCAAAA ACCAAGCAAA GATCTTGCAA AAAAAGAGTC ACAAAAGGTT 
ATCCCAGAAA GCGAATCACA AAAAGTTGTT TCAGAGAAAG ATTTGCAGAA GGGCATCCCA 
GAAAAAGGGT CACAGAAGAG TAACCCGGAA AAAGAGTCGC AGAAGAGTAA CCCGGAAAAA 
GAGTCGCAGA AGAGTAACCC GGAAAAAGAG TCGCAGAAGA GTAACCCGGA AAAAGAGTCG 
CAGAAGAGTA ACCCGGAAAA AGAGTCGCAG AAGAGTAACC CGGAAAAAGA GTCGCAGAAG 
AGTAACCCGG AAAAAGAGTC GCAGAATGTT CTCCCAAGTC AGAAATCTCC TAATAAAGAC 
CAAGAACAAG CAATGGAAAC CGAATCATTC CAGTCATCAA AATCAGTAGA GACCCGTGAA 
ATAATCAAAC CTCATTCTGA CACAGAAAAG CCAGAGAATG CTGAAAAAAC TAAGCTTCAT 
GAAAGCAACA CAGAAAAAGA GTCAACTCAA AAACCATGCA TCTCTGAAGG AGACAAACTC 
TTAACCAGGG GAGAGTCTAT TGGTTCTAAA CCCTGTGAAG ATACGGATAA CCCTCAAGAC 
AAAAAACAAG AGAAGTCTGG CTCCTCCAAA TCGAGAACAG ATACTAATAA ATCATCTGTT 
TCTGATTCAA AAAAGTCAGA AACCAGAAAG AAAGTAGATG CTGAGGACAA ATGTGATGCA 
GCTGAAACGG CTAAAAGTGA CAAATCAGAA ATAGCAGAAG ACAAAGATAA AAAGAGTGAA 
AGTACAAAAA CATCAGATGA AGTTAAAGCT CCAGAAAAGC CCAATAAATC AGATAAAGAA 
CATGAAAAGC AACCAAATCC AATGGAAACT GATTCAGAGA TCCCTAAAAC TGCTGAGGAG 
ACAACAAAAG AAAGCGTGAA GGAAGCCAAT GCTACTGTAG ATAACTGCAC CAGCAAACAA 
TCAGAGCGAG ACGCGACTTC TGAAGAACGC GAGGCTTCCG GTGTTGTTCA AGAAAAGGGG 
AAAGGCATTA AAAAACCAGG ACGGCCTTCA AAGAAATCTA CTGAGAGTCA AAATAAGGAT 
AAAACTGTAG AAGAGGAAAA AGATGATGGA AAGCGACGGG GAAGAACCCC TCTGAAGTTG 
AAGAAACCAG CTCACAGGAG AAAGGCAGAG ATTCAGAGAG AAGAGCTGAA GGGAGATTCG 
GAGTCTGAGA CGACTGATGG ACGGTGTTTG AGAAGGTCTC CCAGAATCTG CAGACCAACC 
GCTAAAGCAG TGGAGATCCA GGATAAGAGG GCGGAGCGGA AACAGGCCAC GCCAGAGAAG 
GAGAAGGAGG AAGAAGAAGA AGAAGAGGAG GAGGAGCCTG TACAGAGGAA ACCGAGGAAA 
GCAGAGCCAG ATGGACAGAC AAAACCAAAG CAGGTGGGGA GACGTCGAAA ACGACAACCG 
TGGTCTCGCA TGAGGCGTAA AAAGAAATCT TCGGAGGATG AGGAAGATGA AGAGGAGAGC 
GATAGCGAAG AAGAGGAGAC AGAGGAGGAT GACAGCGATG AAGACTACAA GGTGGAGAAA 
AGCAAGAAGA GAAGGAGAAA CCGAAACCGC GAACGGCGCA GCTCTGACTC CTCCACCTCT 
TCATCAGAAG ACGATACACC CAATGACGAC CCCTGCAAAC ACTGTGGCCT TCCCAATCAT 
CCCGAACTGA TCCTGCTGTG TGATTCCTGT GACAGCGGCT ACCACACCGC CTGCCTGCGT 
CCTCCGCTCA TGATCATTCC AGACGGAGAG TGGTTCTGTC CTCCCTGCCA GCACAAGCTA 
CTTTGCGACA GACTGGAAGA GCAGCTGCTG AATTTGGATG CCGCCCTCAA AAAGAAAGAA 
CGAGCAGAGC GAAGGAAAGA GCGACTGGTG TATGTTGGCA TTAGTGTGGA GAACATCATC 
ACGCCCTCTG TTGAAGTGGA GGAAGAAAAG GAAGAGGTTT TGGTTAAGGA TAAAGCGGAG 
ACGAAGAAGA GCAAAGGCTG GGGACGGAGA TCAACCAGAG CCAAGAAATA CATAAGCTAC 
AGATTCGATG AGTTTGATGA AGCGATCGAA GAGGCGATTG AGGAGGACAT TAAAGAGGCA 
GAAGGTGGAG GAGCAGGACG AGGGAAGGAC ATGGCGAACA TCACAGGCCA CAGAGGGAAG 
GACATGTCCA CCATCCTGCA GGAGGAAGGC AAAGAGAACG GCCGACCACA AAGAACTAGC 
GCAGCGCAGA AGAAGAAACG GCGGCGTCTC AATGATCTGG ACAGCGACAG CACTGTAGAT 
GAGGAGGAGA GCGAAGATGA GTTTCGCCTC AGCGACAGTT CTGAGGAGGA GTTTGTGGCG 
TCTGAAAACG CTGAAAGTGA AGACAATGCG CCATCCTTAG AGGACAGCGA CTTTGACAGT 
GATGCTCAAG GTTCACGAGT GAATCGTGCG AACAGAAGGA AAACGAGCCG GAGTCGAATC 
CGGAGAAAAC AACCTCCGCG CAGGAGACGC AGACCCAGAG GATACTCTGA TGAAGAGGAG 
CTGGAGACTG ATGAAGAGGA GGAGGAAGAG GACGAGATGG TGACGGAGGG CTCTAGTGAG 
TTCAGTGACA GTGATCTGGA CGTCCGCTGT CGACGCTCGT ATCGCAGCCG AAAGCAGGAG 
GTGAATTACT GTGAAACTTC AGATTCAGAC GGCTCGCAAG CCAGCACCAA CAAAGACAAG 
AAAAAGAAGC GCAGACGACT GTCCAGCTCT GACAGCGAAG CCAGTTTCCA GTCAGCGGAA 
TCGGAAGAAG AAGAGCGAAA ACCCAAGAAG ATCAGAGCCG ACTCGTCTGA GGAGGAGGAA 
TCCAGGAAGC GACGCAGACG TTTATCCCTA AAGCGCCGAC GGGAGTCAGA GGACGATGAC 
GATGATGATG ATTCAGATGA ATCCGAGGAA GAGGAGCGTC CTGTCCGGAA GCGAGTAAAC 
CGAATCGACT CTGACGAAAG TGAGAGCGAA GAAGAGGAGA AGGAGGTGAA GAAGAGTCCG 
CAGGAGAAAG ACACAGAAGA GACGAGCGCT AGTAAAGGTC CCAGTCCAAT AAACGACAAC 
ACAGCGGAGC CTCCTTCTAC TAATGGACAG AGCCCTATAA AGGGTCTGGA GGGCGTCGCG 
CCACGGCCGG GAGCAGGAGC AACGCCTAAA AATGGCAACA CACCATCCAT AGCACCCAAT 
GGCCTGGAAG CCGGCGCACA GGAAGAAGAC GAAGACGATC TTTTGGGCGT CACAGACCTC 
GTAGACTATG TCTGCAACAG CGAACAGTTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rsf1b.1 ( XM_017358079.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213568.1 Danio rerio remodeling and spacing factor 1b, tandem duplicate 1 (rsf1b.1), transcript variant X2, mRNA.

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CloneID	ODa39952
Clone ID Related Accession (Same CDS sequence)	XM_017358079.1 , XM_017358079.2
Accession Version	XM_017358079.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	remodeling and spacing factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017358079.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCCT CGGAGGAAGC GGACGGTTCT TCCCCGGGAT TGTGCCCGAG CTTTGCCGTG 
ATCTGCTCGT TTCTGGAGCG GTACGGAGCT CTGCTGGACC TGCCGGAGGT GTCGTTCCCG 
GAGCTGGAGC GCAGCCTGCA GGACAGCAGC GCGGTACCCA AACTTCTTGT GGACCTTCAC 
GTGAAGTTAC TGAGGAAAAT TGGCAAGTCT GTGTCTGCAG AGAGATGGGA GAAATATCTC 
ATTAAGATTT GTCAGGAGTT TAACACCACA TGGGCATGGG AGTTGGAAAA GAAAGGTTAT 
CAAGAGTTGA CAGTGGAGTG TAAGACAGGA ATATTGAAGT ATTTGTGTGA ATGCCAGTTT 
GATGACAATG TCAAGTTTAA AAATGCTATC AATGAAGAAG ACCCGGATAA AATGCGACTT 
CAGCCTATTG GCCGAGACAA AGACGGCCTC ATGTACTGGT TCCAGCTGGA CCAAGACCAT 
AATGTAAGGA TCTATGTGGA AGAGCAGGAT GATTTGGATG GGTCGTCCTG GAAATGTATA 
GTAAGAAATA GAGATGACCT GGCTCAGATT CTTGGGTTGT TGAAGACTCA GATTGATCCA 
GCACTCCTGG TGAAGAAGGA ACAGGAGGAG GGCTCGTCTA ATAACAGTCC AAATCCCGAT 
GATGAGGAGA ACAAAAAGAA AGAGGGAGCT GAAGTGGAGA AGTCACAAGA TGATTTGTCC 
AAATCTGAAA ATAAGGAAAA CATATTAGAG AAAGAAAATA AAAAGGAAGA AGGTGCAGAT 
GCCACAGATG CTTTGTCGGA AAAGGGTTTA AAGGTAAAAG AGGAGCTGCA GAAACATTCA 
GAGACGGCAA ACGGATCTGA CGTTATTGCT GGATCAATCA AAGAAGAACC ACAGGATGAT 
GAGAAAGTCT TTTCCGCACC TTCAAAGAAA GCAGAACAGA CAGAGGAGGT GAAGAGAAAA 
ACACTAGAAG AGACCCAAAG AGCACTGAAA AATGACCATC AAGCTAAAAT CCCCCTGAAG 
AAGCGAGAGA TGAAGCTAAG TGAAGACTTT GACAGCAACA GCGCAGGACT TCGAAATGCT 
CCAGTAAAAG AGTCAAGGAC GTGTTTCGAT GATAGCATGT CGAGTGCTGA AAAGATCAAT 
GGCCATGTTC CTCACCTTCA GAAAGAAGCA ACAGATTCAG AAAGTCTCTC TCATTTTGAA 
TCCAATGAAA AAACAAAAGA CGCCCAGGTG GATAGAAAGA TGGACCCGAA TGAAACCAGA 
TTTCCTTCTG AAGAGAAGAT GGAAGTAGAG AAACCTAAGG AGAAAAATGA AGCTTCAACT 
AAACAGCACA AAACAACTCC TGGGGAAGAA AAACCAAGTG AACTATCAGA AAAGACACCT 
GATTCTGAAG TAGTAACTGA GAATATGGAA ACTATTGAAG ATATTAAGAC AACACCATGT 
GACGACACAG CAAATGAACT GCCTAAAGAG AAATCAATTA AAGATATTCC AAACAAAGAA 
TCAAAAGAAG ATTCATTGAA AGATGCACAA AGCAAAGAAT CAAAGGGAGA GTCATTGAAA 
GGAAGCAAAG AATCAAAAGG AGAATCATTA TTAAAAGATG CCCCAAGCAA AGAATCAAAA 
GAAGAATCAT TGAAAGATGC GCCGAGCAAA GAGTCAAAAG AATCATCATC AAAAGGTGCC 
CCAAGCAAAG AATCAAAAGG AGAATTATTT AAGGAAAGCA AAGAGTCGAA AAAAGAATCA 
TTAAAAGATA CCCCAAGTAA AGAATCAATA GGAGAATCAT TGAGAGATAC CCAAAGCAAA 
GAGTCAAAAG AATCATCATC AAAAGATGCC CCACGCAAAG AATCAAAAGG AGAATCATTG 
AAGGATGCCC CAAGCAAAGA GTCAAAAGAA GACTCAATGA AAGATACCCC AAGCAAAGAA 
TCAAATGAAT CATTATTGAA GGAAAGCAAA GAGTCAAAAG AAGAATCATT GAAAGATAAC 
CCAAGTAAAG AATCAAAAGA AGAAATATTG AAAGATACCC CAAGCAAAGA ATCAAAACAA 
GAATCATTGA AAGATACCCC AAGCAAAGAA TCAAAGGGAG AATCGTTGAA AGATGCCCCA 
AGCAAAGAAT CAAAAGAATC ATTATTGAAG GAAAGTAAAG AGTCAAAAGA AGAATCGTTG 
AAAGATCCCC CAGGCAAGGA GTCAGCAAAA TCAAAAGATG ACAAAACTAA AGCTCTTTCA 
AAAAAGGAAT CATTGGAAGA TATCTCAAAT GAAAAGTCAG CAAAATCTCT CAAAGATGAT 
GCAACAAAAG ATCCATCAAA AAAAGAATCA TTGCAAGATA TCTCAAACGA GTCAACAAAA 
TCTGTATCAA AAGACGACGC AGCACAAGAT CACTTTAACA AAGAATCATC AAAAGATCTC 
GCAAGCGAAG AGTCGGGGAA AGATCAGCCG GCAAAAGATC TTTCGAATGA AGAGTCTATA 
AAAAATCCTC CAAATCAAAA ACCAAGCAAA GATCTTGCAA AAAAAGAGTC ACAAAAGGTT 
ATCCCAGAAA GCGAATCACA AAAAGTTGTT TCAGAGAAAG ATTTGCAGAA GGGCATCCCA 
GAAAAAGGGT CACAGAAGAG TAACCCGGAA AAAGAGTCGC AGAAGAGTAA CCCGGAAAAA 
GAGTCGCAGA AGAGTAACCC GGAAAAAGAG TCGCAGAAGA GTAACCCGGA AAAAGAGTCG 
CAGAAGAGTA ACCCGGAAAA AGAGTCGCAG AAGAGTAACC CGGAAAAAGA GTCGCAGAAG 
AGTAACCCGG AAAAAGAGTC GCAGAATGTT CTCCCAAGTC AGAAATCTCC TAATAAAGAC 
CAAGAACAAG CAATGGAAAC CGAATCATTC CAGTCATCAA AATCAGTAGA GACCCGTGAA 
ATAATCAAAC CTCATTCTGA CACAGAAAAG CCAGAGAATG CTGAAAAAAC TAAGCTTCAT 
GAAAGCAACA CAGAAAAAGA GTCAACTCAA AAACCATGCA TCTCTGAAGG AGACAAACTC 
TTAACCAGGG GAGAGTCTAT TGGTTCTAAA CCCTGTGAAG ATACGGATAA CCCTCAAGAC 
AAAAAACAAG AGAAGTCTGG CTCCTCCAAA TCGAGAACAG ATACTAATAA ATCATCTGTT 
TCTGATTCAA AAAAGTCAGA AACCAGAAAG AAAGTAGATG CTGAGGACAA ATGTGATGCA 
GCTGAAACGG CTAAAAGTGA CAAATCAGAA ATAGCAGAAG ACAAAGATAA AAAGAGTGAA 
AGTACAAAAA CATCAGATGA AGTTAAAGCT CCAGAAAAGC CCAATAAATC AGATAAAGAA 
CATGAAAAGC AACCAAATCC AATGGAAACT GATTCAGAGA TCCCTAAAAC TGCTGAGGAG 
ACAACAAAAG AAAGCGTGAA GGAAGCCAAT GCTACTGTAG ATAACTGCAC CAGCAAACAA 
TCAGAGCGAG ACGCGACTTC TGAAGAACGC GAGGCTTCCG GTGTTGTTCA AGAAAAGGGG 
AAAGGCATTA AAAAACCAGG ACGGCCTTCA AAGAAATCTA CTGAGAGTCA AAATAAGGAT 
AAAACTGTAG AAGAGGAAAA AGATGATGGA AAGCGACGGG GAAGAACCCC TCTGAAGTTG 
AAGAAACCAG CTCACAGGAG AAAGGCAGAG ATTCAGAGAG AAGAGCTGAA GGGAGATTCG 
GAGTCTGAGA CGACTGATGG ACGGTGTTTG AGAAGGTCTC CCAGAATCTG CAGACCAACC 
GCTAAAGCAG TGGAGATCCA GGATAAGAGG GCGGAGCGGA AACAGGCCAC GCCAGAGAAG 
GAGAAGGAGG AAGAAGAAGA AGAAGAGGAG GAGGAGCCTG TACAGAGGAA ACCGAGGAAA 
GCAGAGCCAG ATGGACAGAC AAAACCAAAG CAGGTGGGGA GACGTCGAAA ACGACAACCG 
TGGTCTCGCA TGAGGCGTAA AAAGAAATCT TCGGAGGATG AGGAAGATGA AGAGGAGAGC 
GATAGCGAAG AAGAGGAGAC AGAGGAGGAT GACAGCGATG AAGACTACAA GGTGGAGAAA 
AGCAAGAAGA GAAGGAGAAA CCGAAACCGC GAACGGCGCA GCTCTGACTC CTCCACCTCT 
TCATCAGAAG ACGATACACC CAATGACGAC CCCTGCAAAC ACTGTGGCCT TCCCAATCAT 
CCCGAACTGA TCCTGCTGTG TGATTCCTGT GACAGCGGCT ACCACACCGC CTGCCTGCGT 
CCTCCGCTCA TGATCATTCC AGACGGAGAG TGGTTCTGTC CTCCCTGCCA GCACAAGCTA 
CTTTGCGACA GACTGGAAGA GCAGCTGCTG AATTTGGATG CCGCCCTCAA AAAGAAAGAA 
CGAGCAGAGC GAAGGAAAGA GCGACTGGTG TATGTTGGCA TTAGTGTGGA GAACATCATC 
ACGCCCTCTG TTGAAGTGGA GGAAGAAAAG GAAGAGGTTT TGGTTAAGGA TAAAGCGGAG 
ACGAAGAAGA GCAAAGGCTG GGGACGGAGA TCAACCAGAG CCAAGAAATA CATAAGCTAC 
AGATTCGATG AGTTTGATGA AGCGATCGAA GAGGCGATTG AGGAGGACAT TAAAGAGGCA 
GAAGGTGGAG GAGCAGGACG AGGGAAGGAC ATGGCGAACA TCACAGGCCA CAGAGGGAAG 
GACATGTCCA CCATCCTGCA GGAGGAAGGC AAAGAGAACG GCCGACCACA AAGAACTAGC 
GCAGCGCAGA AGAAGAAACG GCGGCGTCTC AATGATCTGG ACAGCGACAG CACTGTAGAT 
GAGGAGGAGA GCGAAGATGA GTTTCGCCTC AGCGACAGTT CTGAGGAGGA GTTTGTGGCG 
TCTGAAAACG CTGAAAGTGA AGACAATGCG CCATCCTTAG AGGACAGCGA CTTTGACAGT 
GATGCTCAAG GTTCACGAGT GAATCGTGCG AACAGAAGGA AAACGAGCCG GAGTCGAATC 
CGGAGAAAAC AACCTCCGCG CAGGAGACGC AGACCCAGAG GATACTCTGA TGAAGAGGAG 
CTGGAGACTG ATGAAGAGGA GGAGGAAGAG GACGAGATGG TGACGGAGGG CTCTAGTGAG 
TTCAGTGACA GTGATCTGGA CGTCCGCTGT CGACGCTCGT ATCGCAGCCG AAAGCAGGAG 
GTGAATTACT GTGAAACTTC AGATTCAGAC GGCTCGCAAG CCAGCACCAA CAAAGACAAG 
AAAAAGAAGC GCAGACGACT GTCCAGCTCT GACAGCGAAG CCAGTTTCCA GTCAGCGGAA 
TCGGAAGAAG AAGAGCGAAA ACCCAAGAAG ATCAGAGCCG ACTCGTCTGA GGAGGAGGAA 
TCCAGGAAGC GACGCAGACG TTTATCCCTA AAGCGCCGAC GGGAGTCAGA GGACGATGAC 
GATGATGATG ATTCAGATGA ATCCGAGGAA GAGGAGCGTC CTGTCCGGAA GCGAGTAAAC 
CGAATCGACT CTGACGAAAG TGAGAGCGAA GAAGAGGAGA AGGAGGTGAA GAAGAGTCCG 
CAGGAGAAAG ACACAGAAGA GACGAGCGCT AGTAAAGGTC CCAGTCCAAT AAACGACAAC 
ACAGCGGAGC CTCCTTCTAC TAATGGACAG AGCCCTATAA AGGGTCTGGA GGGCGTCGCG 
CCACGGCCGG GAGCAGGAGC AACGCCTAAA AATGGCAACA CACCATCCAT AGCACCCAAT 
GGCCTGGAAG CCGGCGCACA GGAAGAAGAC GAAGACGATC TTTTGGGCGT CACAGACCTC 
GTAGACTATG TCTGCAACAG CGAACAGTTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213568.1
CDS	94..5826
Translation	MSASEEADGSSPGLCPSFAVICSFLERYGALLDLPEVSFPELERSLQDSSAVPKLLVDLHVKLLRKIGKSVSAERWEKYLIKICQEFNTTWAWELEKKGYQELTVECKTGILKYLCECQFDDNVKFKNAINEEDPDKMRLQPIGRDKDGLMYWFQLDQDHNVRIYVEEQDDLDGSSWKCIVRNRDDLAQILGLLKTQIDPALLVKKEQEEGSSNNSPNPDDEENKKKEGAEVEKSQDDLSKSENKENILEKENKKEEGADATDALSEKGLKVKEELQKHSETANGSDVIAGSIKEEPQDDEKVFSAPSKKAEQTEEVKRKTLEETQRALKNDHQAKIPLKKREMKLSEDFDSNSAGLRNAPVKESRTCFDDSMSSAEKINGHVPHLQKEATDSESLSHFESNEKTKDAQVDRKMDPNETRFPSEEKMEVEKPKEKNEASTKQHKTTPGEEKPSELSEKTPDSEVVTENMETIEDIKTTPCDDTANELPKEKSIKDIPNKESKEDSLKDAQSKESKGESLKGSKESKGESLLKDAPSKESKEESLKDAPSKESKESSSKGAPSKESKGELFKESKESKKESLKDTPSKESIGESLRDTQSKESKESSSKDAPRKESKGESLKDAPSKESKEDSMKDTPSKESNESLLKESKESKEESLKDNPSKESKEEILKDTPSKESKQESLKDTPSKESKGESLKDAPSKESKESLLKESKESKEESLKDPPGKESAKSKDDKTKALSKKESLEDISNEKSAKSLKDDATKDPSKKESLQDISNESTKSVSKDDAAQDHFNKESSKDLASEESGKDQPAKDLSNEESIKNPPNQKPSKDLAKKESQKVIPESESQKVVSEKDLQKGIPEKGSQKSNPEKESQKSNPEKESQKSNPEKESQKSNPEKESQKSNPEKESQKSNPEKESQKSNPEKESQNVLPSQKSPNKDQEQAMETESFQSSKSVETREIIKPHSDTEKPENAEKTKLHESNTEKESTQKPCISEGDKLLTRGESIGSKPCEDTDNPQDKKQEKSGSSKSRTDTNKSSVSDSKKSETRKKVDAEDKCDAAETAKSDKSEIAEDKDKKSESTKTSDEVKAPEKPNKSDKEHEKQPNPMETDSEIPKTAEETTKESVKEANATVDNCTSKQSERDATSEEREASGVVQEKGKGIKKPGRPSKKSTESQNKDKTVEEEKDDGKRRGRTPLKLKKPAHRRKAEIQREELKGDSESETTDGRCLRRSPRICRPTAKAVEIQDKRAERKQATPEKEKEEEEEEEEEEPVQRKPRKAEPDGQTKPKQVGRRRKRQPWSRMRRKKKSSEDEEDEEESDSEEEETEEDDSDEDYKVEKSKKRRRNRNRERRSSDSSTSSSEDDTPNDDPCKHCGLPNHPELILLCDSCDSGYHTACLRPPLMIIPDGEWFCPPCQHKLLCDRLEEQLLNLDAALKKKERAERRKERLVYVGISVENIITPSVEVEEEKEEVLVKDKAETKKSKGWGRRSTRAKKYISYRFDEFDEAIEEAIEEDIKEAEGGGAGRGKDMANITGHRGKDMSTILQEEGKENGRPQRTSAAQKKKRRRLNDLDSDSTVDEEESEDEFRLSDSSEEEFVASENAESEDNAPSLEDSDFDSDAQGSRVNRANRRKTSRSRIRRKQPPRRRRRPRGYSDEEELETDEEEEEEDEMVTEGSSEFSDSDLDVRCRRSYRSRKQEVNYCETSDSDGSQASTNKDKKKKRRRLSSSDSEASFQSAESEEEERKPKKIRADSSEEEESRKRRRRLSLKRRRESEDDDDDDDSDESEEEERPVRKRVNRIDSDESESEEEEKEVKKSPQEKDTEETSASKGPSPINDNTAEPPSTNGQSPIKGLEGVAPRPGAGATPKNGNTPSIAPNGLEAGAQEEDEDDLLGVTDLVDYVCNSEQL

Target ORF information:

RefSeq Version	XM_017358079.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio remodeling and spacing factor 1b, tandem duplicate 1 (rsf1b.1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358079.2

ORF Insert Sequence:

ATGTCTGCCT CGGAGGAAGC GGACGGTTCT TCCCCGGGAT TGTGCCCGAG CTTTGCCGTG 
ATCTGCTCGT TTCTGGAGCG GTACGGAGCT CTGCTGGACC TGCCGGAGGT GTCGTTCCCG 
GAGCTGGAGC GCAGCCTGCA GGACAGCAGC GCGGTACCCA AACTTCTTGT GGACCTTCAC 
GTGAAGTTAC TGAGGAAAAT TGGCAAGTCT GTGTCTGCAG AGAGATGGGA GAAATATCTC 
ATTAAGATTT GTCAGGAGTT TAACACCACA TGGGCATGGG AGTTGGAAAA GAAAGGTTAT 
CAAGAGTTGA CAGTGGAGTG TAAGACAGGA ATATTGAAGT ATTTGTGTGA ATGCCAGTTT 
GATGACAATG TCAAGTTTAA AAATGCTATC AATGAAGAAG ACCCGGATAA AATGCGACTT 
CAGCCTATTG GCCGAGACAA AGACGGCCTC ATGTACTGGT TCCAGCTGGA CCAAGACCAT 
AATGTAAGGA TCTATGTGGA AGAGCAGGAT GATTTGGATG GGTCGTCCTG GAAATGTATA 
GTAAGAAATA GAGATGACCT GGCTCAGATT CTTGGGTTGT TGAAGACTCA GATTGATCCA 
GCACTCCTGG TGAAGAAGGA ACAGGAGGAG GGCTCGTCTA ATAACAGTCC AAATCCCGAT 
GATGAGGAGA ACAAAAAGAA AGAGGGAGCT GAAGTGGAGA AGTCACAAGA TGATTTGTCC 
AAATCTGAAA ATAAGGAAAA CATATTAGAG AAAGAAAATA AAAAGGAAGA AGGTGCAGAT 
GCCACAGATG CTTTGTCGGA AAAGGGTTTA AAGGTAAAAG AGGAGCTGCA GAAACATTCA 
GAGACGGCAA ACGGATCTGA CGTTATTGCT GGATCAATCA AAGAAGAACC ACAGGATGAT 
GAGAAAGTCT TTTCCGCACC TTCAAAGAAA GCAGAACAGA CAGAGGAGGT GAAGAGAAAA 
ACACTAGAAG AGACCCAAAG AGCACTGAAA AATGACCATC AAGCTAAAAT CCCCCTGAAG 
AAGCGAGAGA TGAAGCTAAG TGAAGACTTT GACAGCAACA GCGCAGGACT TCGAAATGCT 
CCAGTAAAAG AGTCAAGGAC GTGTTTCGAT GATAGCATGT CGAGTGCTGA AAAGATCAAT 
GGCCATGTTC CTCACCTTCA GAAAGAAGCA ACAGATTCAG AAAGTCTCTC TCATTTTGAA 
TCCAATGAAA AAACAAAAGA CGCCCAGGTG GATAGAAAGA TGGACCCGAA TGAAACCAGA 
TTTCCTTCTG AAGAGAAGAT GGAAGTAGAG AAACCTAAGG AGAAAAATGA AGCTTCAACT 
AAACAGCACA AAACAACTCC TGGGGAAGAA AAACCAAGTG AACTATCAGA AAAGACACCT 
GATTCTGAAG TAGTAACTGA GAATATGGAA ACTATTGAAG ATATTAAGAC AACACCATGT 
GACGACACAG CAAATGAACT GCCTAAAGAG AAATCAATTA AAGATATTCC AAACAAAGAA 
TCAAAAGAAG ATTCATTGAA AGATGCACAA AGCAAAGAAT CAAAGGGAGA GTCATTGAAA 
GGAAGCAAAG AATCAAAAGG AGAATCATTA TTAAAAGATG CCCCAAGCAA AGAATCAAAA 
GAAGAATCAT TGAAAGATGC GCCGAGCAAA GAGTCAAAAG AATCATCATC AAAAGGTGCC 
CCAAGCAAAG AATCAAAAGG AGAATTATTT AAGGAAAGCA AAGAGTCGAA AAAAGAATCA 
TTAAAAGATA CCCCAAGTAA AGAATCAATA GGAGAATCAT TGAGAGATAC CCAAAGCAAA 
GAGTCAAAAG AATCATCATC AAAAGATGCC CCACGCAAAG AATCAAAAGG AGAATCATTG 
AAGGATGCCC CAAGCAAAGA GTCAAAAGAA GACTCAATGA AAGATACCCC AAGCAAAGAA 
TCAAATGAAT CATTATTGAA GGAAAGCAAA GAGTCAAAAG AAGAATCATT GAAAGATAAC 
CCAAGTAAAG AATCAAAAGA AGAAATATTG AAAGATACCC CAAGCAAAGA ATCAAAACAA 
GAATCATTGA AAGATACCCC AAGCAAAGAA TCAAAGGGAG AATCGTTGAA AGATGCCCCA 
AGCAAAGAAT CAAAAGAATC ATTATTGAAG GAAAGTAAAG AGTCAAAAGA AGAATCGTTG 
AAAGATCCCC CAGGCAAGGA GTCAGCAAAA TCAAAAGATG ACAAAACTAA AGCTCTTTCA 
AAAAAGGAAT CATTGGAAGA TATCTCAAAT GAAAAGTCAG CAAAATCTCT CAAAGATGAT 
GCAACAAAAG ATCCATCAAA AAAAGAATCA TTGCAAGATA TCTCAAACGA GTCAACAAAA 
TCTGTATCAA AAGACGACGC AGCACAAGAT CACTTTAACA AAGAATCATC AAAAGATCTC 
GCAAGCGAAG AGTCGGGGAA AGATCAGCCG GCAAAAGATC TTTCGAATGA AGAGTCTATA 
AAAAATCCTC CAAATCAAAA ACCAAGCAAA GATCTTGCAA AAAAAGAGTC ACAAAAGGTT 
ATCCCAGAAA GCGAATCACA AAAAGTTGTT TCAGAGAAAG ATTTGCAGAA GGGCATCCCA 
GAAAAAGGGT CACAGAAGAG TAACCCGGAA AAAGAGTCGC AGAAGAGTAA CCCGGAAAAA 
GAGTCGCAGA AGAGTAACCC GGAAAAAGAG TCGCAGAAGA GTAACCCGGA AAAAGAGTCG 
CAGAAGAGTA ACCCGGAAAA AGAGTCGCAG AAGAGTAACC CGGAAAAAGA GTCGCAGAAG 
AGTAACCCGG AAAAAGAGTC GCAGAATGTT CTCCCAAGTC AGAAATCTCC TAATAAAGAC 
CAAGAACAAG CAATGGAAAC CGAATCATTC CAGTCATCAA AATCAGTAGA GACCCGTGAA 
ATAATCAAAC CTCATTCTGA CACAGAAAAG CCAGAGAATG CTGAAAAAAC TAAGCTTCAT 
GAAAGCAACA CAGAAAAAGA GTCAACTCAA AAACCATGCA TCTCTGAAGG AGACAAACTC 
TTAACCAGGG GAGAGTCTAT TGGTTCTAAA CCCTGTGAAG ATACGGATAA CCCTCAAGAC 
AAAAAACAAG AGAAGTCTGG CTCCTCCAAA TCGAGAACAG ATACTAATAA ATCATCTGTT 
TCTGATTCAA AAAAGTCAGA AACCAGAAAG AAAGTAGATG CTGAGGACAA ATGTGATGCA 
GCTGAAACGG CTAAAAGTGA CAAATCAGAA ATAGCAGAAG ACAAAGATAA AAAGAGTGAA 
AGTACAAAAA CATCAGATGA AGTTAAAGCT CCAGAAAAGC CCAATAAATC AGATAAAGAA 
CATGAAAAGC AACCAAATCC AATGGAAACT GATTCAGAGA TCCCTAAAAC TGCTGAGGAG 
ACAACAAAAG AAAGCGTGAA GGAAGCCAAT GCTACTGTAG ATAACTGCAC CAGCAAACAA 
TCAGAGCGAG ACGCGACTTC TGAAGAACGC GAGGCTTCCG GTGTTGTTCA AGAAAAGGGG 
AAAGGCATTA AAAAACCAGG ACGGCCTTCA AAGAAATCTA CTGAGAGTCA AAATAAGGAT 
AAAACTGTAG AAGAGGAAAA AGATGATGGA AAGCGACGGG GAAGAACCCC TCTGAAGTTG 
AAGAAACCAG CTCACAGGAG AAAGGCAGAG ATTCAGAGAG AAGAGCTGAA GGGAGATTCG 
GAGTCTGAGA CGACTGATGG ACGGTGTTTG AGAAGGTCTC CCAGAATCTG CAGACCAACC 
GCTAAAGCAG TGGAGATCCA GGATAAGAGG GCGGAGCGGA AACAGGCCAC GCCAGAGAAG 
GAGAAGGAGG AAGAAGAAGA AGAAGAGGAG GAGGAGCCTG TACAGAGGAA ACCGAGGAAA 
GCAGAGCCAG ATGGACAGAC AAAACCAAAG CAGGTGGGGA GACGTCGAAA ACGACAACCG 
TGGTCTCGCA TGAGGCGTAA AAAGAAATCT TCGGAGGATG AGGAAGATGA AGAGGAGAGC 
GATAGCGAAG AAGAGGAGAC AGAGGAGGAT GACAGCGATG AAGACTACAA GGTGGAGAAA 
AGCAAGAAGA GAAGGAGAAA CCGAAACCGC GAACGGCGCA GCTCTGACTC CTCCACCTCT 
TCATCAGAAG ACGATACACC CAATGACGAC CCCTGCAAAC ACTGTGGCCT TCCCAATCAT 
CCCGAACTGA TCCTGCTGTG TGATTCCTGT GACAGCGGCT ACCACACCGC CTGCCTGCGT 
CCTCCGCTCA TGATCATTCC AGACGGAGAG TGGTTCTGTC CTCCCTGCCA GCACAAGCTA 
CTTTGCGACA GACTGGAAGA GCAGCTGCTG AATTTGGATG CCGCCCTCAA AAAGAAAGAA 
CGAGCAGAGC GAAGGAAAGA GCGACTGGTG TATGTTGGCA TTAGTGTGGA GAACATCATC 
ACGCCCTCTG TTGAAGTGGA GGAAGAAAAG GAAGAGGTTT TGGTTAAGGA TAAAGCGGAG 
ACGAAGAAGA GCAAAGGCTG GGGACGGAGA TCAACCAGAG CCAAGAAATA CATAAGCTAC 
AGATTCGATG AGTTTGATGA AGCGATCGAA GAGGCGATTG AGGAGGACAT TAAAGAGGCA 
GAAGGTGGAG GAGCAGGACG AGGGAAGGAC ATGGCGAACA TCACAGGCCA CAGAGGGAAG 
GACATGTCCA CCATCCTGCA GGAGGAAGGC AAAGAGAACG GCCGACCACA AAGAACTAGC 
GCAGCGCAGA AGAAGAAACG GCGGCGTCTC AATGATCTGG ACAGCGACAG CACTGTAGAT 
GAGGAGGAGA GCGAAGATGA GTTTCGCCTC AGCGACAGTT CTGAGGAGGA GTTTGTGGCG 
TCTGAAAACG CTGAAAGTGA AGACAATGCG CCATCCTTAG AGGACAGCGA CTTTGACAGT 
GATGCTCAAG GTTCACGAGT GAATCGTGCG AACAGAAGGA AAACGAGCCG GAGTCGAATC 
CGGAGAAAAC AACCTCCGCG CAGGAGACGC AGACCCAGAG GATACTCTGA TGAAGAGGAG 
CTGGAGACTG ATGAAGAGGA GGAGGAAGAG GACGAGATGG TGACGGAGGG CTCTAGTGAG 
TTCAGTGACA GTGATCTGGA CGTCCGCTGT CGACGCTCGT ATCGCAGCCG AAAGCAGGAG 
GTGAATTACT GTGAAACTTC AGATTCAGAC GGCTCGCAAG CCAGCACCAA CAAAGACAAG 
AAAAAGAAGC GCAGACGACT GTCCAGCTCT GACAGCGAAG CCAGTTTCCA GTCAGCGGAA 
TCGGAAGAAG AAGAGCGAAA ACCCAAGAAG ATCAGAGCCG ACTCGTCTGA GGAGGAGGAA 
TCCAGGAAGC GACGCAGACG TTTATCCCTA AAGCGCCGAC GGGAGTCAGA GGACGATGAC 
GATGATGATG ATTCAGATGA ATCCGAGGAA GAGGAGCGTC CTGTCCGGAA GCGAGTAAAC 
CGAATCGACT CTGACGAAAG TGAGAGCGAA GAAGAGGAGA AGGAGGTGAA GAAGAGTCCG 
CAGGAGAAAG ACACAGAAGA GACGAGCGCT AGTAAAGGTC CCAGTCCAAT AAACGACAAC 
ACAGCGGAGC CTCCTTCTAC TAATGGACAG AGCCCTATAA AGGGTCTGGA GGGCGTCGCG 
CCACGGCCGG GAGCAGGAGC AACGCCTAAA AATGGCAACA CACCATCCAT AGCACCCAAT 
GGCCTGGAAG CCGGCGCACA GGAAGAAGAC GAAGACGATC TTTTGGGCGT CACAGACCTC 
GTAGACTATG TCTGCAACAG CGAACAGTTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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rsf1b.1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

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