dicer1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	dicer1
Entrez Gene ID	324724
Full Name	dicer 1, ribonuclease type III
Synonyms	fc39d11,fc74b05,wu:fc39d11,wu:fc74b05
General protein information	Preferred Names dicer 1, ribonuclease type III Names endoribonuclease Dicer Dicer1, Dcr-1 homolog dsRNA-specific ribonuclease
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005158665.3	XP_005158722.1	endoribonuclease Dicer isoform X1
XM_005158666.3	XP_005158723.1	endoribonuclease Dicer isoform X1
XM_021467115.1	XP_021322790.1	endoribonuclease Dicer isoform X1
XM_005158665.4	XP_005158722.1	endoribonuclease Dicer isoform X1
XM_021467114.1	XP_021322789.1	endoribonuclease Dicer isoform X1
XM_021467116.1	XP_021322791.1	endoribonuclease Dicer isoform X1
NM_001161453.2	NP_001154925.1	endoribonuclease Dicer
XM_005158666.4	XP_005158723.1	endoribonuclease Dicer isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	DICER1	NP_085124.2
Rattus norvegicus (Norway rat)	Dicer1	XP_006240573.1
Danio rerio (zebrafish)	dicer1	NP_001154925.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002836	XP_003436256.1
Magnaporthe oryzae (rice blast fungus)	MGG_01541	XP_003714515.1
Neurospora crassa	NCU08270	XP_961898.1
	DICER1	XP_001100868.1
Gallus gallus (chicken)	DICER1	NP_001035555.1
Drosophila melanogaster (fruit fly)	Dcr-1	NP_524453.1
Caenorhabditis elegans (roundworm)	dcr-1	NP_498761.2
Xenopus tropicalis (tropical clawed frog)	dicer1	NP_001123390.2
Canis lupus familiaris (dog)	DICER1	XP_868526.3
Bos taurus (cattle)	DICER1	NP_976235.1
Schizosaccharomyces pombe (fission yeast)	dcr1	NP_588215.2
Pan troglodytes (chimpanzee)	DICER1	XP_001154369.1
Mus musculus (house mouse)	Dicer1	NP_683750.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Histological Characterization of the Dicer1 Mutant Zebrafish Retina.
Akhtar S, Patnaik SR, Kotapati Raghupathy R, Al-Mubrad TM, Craft JA, Shu X
Journal of ophthalmology2015309510(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Dynamic miRNA expression patterns during retinal regeneration in zebrafish: reduced dicer or miRNA expression suppresses proliferation of Muller glia-derived neuronal progenitor cells
Title: Dynamic miRNA expression patterns during retinal regeneration in zebrafish: reduced dicer or miRNA expression suppresses proliferation of M?ller glia-derived neuronal progenitor cells.

The following dicer1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dicer1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa03830	NM_001161453.2 Latest version!	Danio rerio dicer 1, ribonuclease type III (dicer1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa03830	XM_005158665.4 Latest version!	Danio rerio dicer 1, ribonuclease type III (dicer1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa03830	XM_005158666.4 Latest version!	Danio rerio dicer 1, ribonuclease type III (dicer1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa03830	XM_021467115.1 Latest version!	Danio rerio dicer 1, ribonuclease type III (dicer1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa03830	XM_021467116.1 Latest version!	Danio rerio dicer 1, ribonuclease type III (dicer1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa03830	XM_021467114.1 Latest version!	Danio rerio dicer 1, ribonuclease type III (dicer1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
ODa03830	XM_005158665.3	Danio rerio dicer 1, ribonuclease type III (dicer1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa03830	XM_005158666.3	Danio rerio dicer 1, ribonuclease type III (dicer1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dicer1 ( NM_001161453.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001154925.1 Danio rerio dicer 1, ribonuclease type III (dicer1), mRNA.

Price & Availability↑

CloneID	ODa03830
Clone ID Related Accession (Same CDS sequence)	XM_005158665.3 , XM_005158666.3 , XM_021467115.1 , XM_005158665.4 , XM_021467114.1 , XM_021467116.1 , NM_001161453.2 , XM_005158666.4
Accession Version	NM_001161453.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5598bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-14
Organism	Danio rerio(zebrafish)
Product	endoribonuclease Dicer
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL772219.7. On Oct 26, 2010 this sequence version replaced NM_001161453.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: GDQH01002492.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCTGGCC TACAGCTGGT GACCCCTGCC TCCTCACCCA TGGGGCCCTT CTTTGGTCTC 
CCGTGGCAAC AGGAAGCTAT CCATGACAAC ATATACACAC CAAGGAAATA TCAGGTTGAA 
CTTCTTGAAG CAGCTCTTGA ACATAATACT ATAGTCTGCT TAAATACTGG CTCAGGGAAG 
ACCTTTATCG CAGTGCTCCT AATCAAAGAA CTCTCCCACC AAATTCGTGG CGAGAATGGG 
AAGAGGACAG TTTTCCTGGT GAATGCAGCG TCATCTGTGG CTCAGCAAGC ATCTACTGTA 
AGAACACACT CTGATCTCCA AGTAGGCGAC TATATGTCAG AGGACATGAC ATCATGGCCC 
GAGGAGATGT GGAACAGAGA GATGATAGAG AATCAGGTGC TGGTGATGAC GTGCCACATC 
TTCCTGCATG TTTTAAAGAA TGGAGTCTTG CCACTATCAA AGATCAACCT GCTGGTTTTT 
GACGAATGCC ACCTTGCAAT CACTGGCCAT CCTTATCGGG AAATAATGAA GATTTGTGAA 
GGTTGCCCCA GCTGTCCTCG CATTTTGGGT CTCACTGCTT CCATTTTAAA TGGCAAATGT 
GACCCTTGTG ATTTGGAGGA GAAAATTCAG AACCTGGAGA AGATTCTTCA GAGCAATGCA 
GAAACTGCCA CTGATCTTGT GGTGCTGGAT AGGTATGCGT CGCAGCCGCG TGAGGAGGTG 
CTGGATTGTG GACAGTACCA AGATCAGAGT GGGCTCTCGG AGAGGCTTTT AAATGAGCTG 
GACGAAGCCC TCAATTTTCT AAACGATTGC AACTTGTCTG TACATAGAGA AGATAGAGAC 
CCCACGTTTA TCTCCAAACA GGTGCTGAAT GACTGTCGTG CTGTGTTGAC TGTGCTGGGC 
CCTTGGTGTG CGGATAAAGC TGCAGGAATC ATGGTTCGGG AGCTGCAGAA ATATATTAAA 
CATGAACAGG AAGAGCTAAA CCGCAAGTTC CTGTTGTTCA CCGACACCAT CCTGAGGAAA 
ATCCATGCCT TATGTGAGGA GCATTTTTCC CCAGCTTCCC TCGACCTGAA GTTTGTCACT 
CCCAAGGTCA TCAGACTTTT GGAGATTCTC CACGAGTACA AGCCTTTCGA ACGGCAGCAG 
TTTGAGAGTG TGGAGTGGTA CAACAATCGC AATCAGGACA ATTATGTTTC TTGGAGTGAT 
TCTGAAGATG ATGATGAGGA CGAAGAGGCG GAGGCAAAGG AGAAAACCGA AGCAAATTTT 
CCCTCGCCGT TCACAAATAT CCTCTGTGGA ATCATCTTTG TGGAAAGGCG ATATACAGCG 
GTTGTTTTAA ACAGACTTAT TAAGGAGGCA GGAAAGCAAG ACCCAGAGCT GGCTTACATC 
AGTAGCAACT TCATCACAGG GCACAGCATC GGCAAGAACC AGCCTCGCAA CAAGCAGATG 
GAGGTGGAGT TCAGAAAACA AGAGGAAGTC TTGAGGAAAT TCAGAGCCCA TGAAACCAAC 
TTGCTCATTG CAACCAGCAT TGTGGAGGAA GGCGTCGATA TCCCAAAGTG CAATTTGGTC 
GTTCGGTTTG ACCTGCCGAC AGAGTACCGG TCGTACGTGC AATCCAAAGG TCGAGCCCGT 
GCTCCAGTCT CCAACTACAT CATGCTAGCA GACAGCGAAC GGACTAAAAC CTTCCAAGAA 
GACCTCAAGA CCTACAAGGC CATAGAGAAG ATCCTCCGAA ATAAGTGCTC GAAGTCGGCT 
GAATGCAATG ATTTTGAGCT GGAGCCTGTG ACCGATGACG ACAATGTTCT CCCACCGTAC 
GTCCTGCGCT CAGAGGACGG CGGGCCTCGT GTCACCATGA ACACTGCCAT TGGGCACGTC 
AACCGATATT GTGCGCGCTT GCCCAGTGAT CCGTTCACTC ATCTCGCTCC GAAGTGTAAA 
ACTGTAGAGA TGAACACCGG AGGGTATCGA TCCACACTCT TCCTACCTAT CAACTCACCA 
CTCAGAGTTC CTGTCACCGG GCCAGTAATG AATTGTGCAA GACTGGCTGA GAAAGCTGTC 
GCTCTTCTCT GTTGTGAAAA ACTCCACAAA ATTGGTGAAC TGGATGATCA TCTGATGCCT 
GTGGGAAAGG AAACTGTGAA GTATGAGGAG GAGCTGGACC TGCATGATGA AGAGGAGACA 
AGCGTGCCAG GAAGACCAGG CTCCACTAAA CGGAGACAGT GCTCCCCAAA AGCCATACCA 
GAGTGTCTGC GGGGTTGTTA CCCTGTGCCA GAGCAGCCTT GCTACCTTTA CGTTATTGGG 
ATGGTCCTCA CCACCCCTCT ACCTGACGAA CTCAACTTCC GGCGGAGGAA GCTGTATCCA 
CCAGAGGACA CAACCCGATG TTTTGGCATT CTGACAGCTA AACCAATACC CCGGATCCCT 
CATTTCCCTG TGTACACTCG TTCTGGTGAG GTGACCATCT CCATTGAGCT GCAGAAGTCG 
GGCTTTAGTC TAAGTGCGGA GCAGCTGGAG CTGATCACAC GTCTGCATCA GTACATCTTC 
TCCCACATCC TCCGTCTGGA AAAGCCAGCA CTAGAGTTTA AGCCTGTAGA GGCAGATTCT 
GCCTACTGTG TTCTACCTCT CAACATCGTT GAGGATTCCA ATACTTTGGA TCTGGATTTC 
AAGTTCATGG AGGATATTGA AAAGTCTGAA GCACGCATTG GCATTCCAAA CACTCAGTAC 
ACCAAGCAAA ACCCATTCAT CTTCAAATTG GAGGACTACC AGGATGCTGT CATCATTCCA 
AGGTATCGTA ATTTTGACCA GCCGCATCGG TTCTACGTGG CTGACGTTTA CACAGACCTC 
ACCCCGCTCA GTAAATTCCC CTCTCCAGAG TACGAGACTT TTGCCGAATA CTATAAAACC 
AAGTACAACC TGGACCTGTC AAATGTTAAT CAGCCCCTAC TGGATGTGGA CCACACATCT 
TCAAGATTGA ATCTTTTAAC TCCCCGGCAC CTTAATCAAA AGGGGAAAGC CCTTCCTCTC 
AGCAGTGCGG AGAAGAGAAA GGCCAAGTGG GAAAGCCTAC AAAACAAACA GATTTTAGTT 
CCGGAGCTCT GTGCCATTCA CCCCATCCCA GCCTCTTTGT GGAGGAAAGC CGTCTGCCTT 
CCCAGCATTC TCTATAGACT TCACTGCCTT CTGACGGCCG AAGAGCTGCG GTCTCAGACA 
GCCATCGATG CTGGCGTAGG GGCTCAGACC TTGCCTCCTG ATTTCAGGTA TCCTAATTTG 
GATTTCGGAT GGAAGAAATC CATCGACAGC AAGTCTTTCA TCTCCTGCCC CAGTGCATGT 
ATGGAAGAAG ACGACGATCA CTGTAAACTC GGCACAAGCT CCGATTCAAA CCACACTGCC 
CCCGAGAGCT GCAGTATGGA GGTCTCCCAG CCTCCAGAAG GCGCTCCCAA CACACCAGAT 
GAAAAGCTAG AAACCCTCAC TCTCCCTGTG ACTGATCTAA ACAAAGACTG TTTCCCAAAC 
CTCCCAAACG GCACTCAGGC CGACAGCGAC GACCTCCCTC ACAGAAGTGA CGTTTGCCAA 
TGCTCTCAGC TGGGCCCTCT GGAGAGAGAC CTATCAACAC AAACCACTAC CTCAGTTTCA 
GTGCGGCCCT CTCCAGCTGG AGAACCACAG CCATGGCCCA GCGATGAATG TACAGGCAGG 
AGCTCGGATC TCTGCGACCC ACATGTGAAG AAACCTACCT CAAAGCATTG CCCTAAATCG 
GAGACGGCGA CTAGCACCCC AGCACCTTCA GAAACGTCAT CAGAGGACTG TAGATCAGCC 
TGCGCTGGTC CAGCCTGGGA TTCGCCCAAA ACCCTGGGCC CGAACCCCGG GCTCATCCTG 
CAGGCCCTGA CTCTCTCCAA CGCCAGTGAC GGCTTCAATC TGGAACGCCT GGAGATGCTG 
GGCGACTCCT TCCTGAAACA CGCCATCACC ACATACCTGT TCTGCACTTA CCCTGACGCC 
CACGAGGGCA GACTGTCCTA CATGAGAAGC AAGAAGGTCA GCAACTGTAA CCTGTATCGG 
CTGGGGAAGA AGAAGGGTCT TCCGAGTCGA ATGGTGGTGT CCATCTTTGA CCCGCCGGTA 
AACTGGCTTC CTCCAGGCTA CGTCGTGAAT CAGGACAAAA GCAGCACAGA CAAGTGGGAC 
TCAGATGAGA ATAAAGATTT AGCGAATGGA AAAGCCAGCG ATGACGAAGA CGAGGATGAC 
GATGATGAGC CGGAAGAAGC AGAGGTCGAG CCATCTAAAG AAGATGTAAA TGTTGAAGAC 
GACCTGGAGT ATTATTACGA ACACATCCGC TTCATCGACA GCATGTTAAT CGGCTCTGGC 
GCTTTCGGCA AGAAGATCTC CCTCCAGCCC ACAGACCCCG GCTACGAATG GAAAGCTCCT 
AAAAAAGCCC ACAACTCCCA CTTCTCGCCA GACGGGGGTG CTGACGAGTT CGATTACAGC 
TCCTGGGACG CCATGTGTTA CCTGGACCCC AGTAAAGCAG GTGAGGAGGA CGATTTCGTG 
GTGGGCTTCT GGAATCCCTC TGAGGAAAAC TGCGGCACGG ACATCGGGAA ACAGTCCATC 
TCTTATGACC TTCACACGGA GCAGTGCATC GCAGATAAGA GCATAGCGGA CTGTGTGGAG 
GCTCTGCTGG GCTGCTATCT GACCAGCTGT GGTGAGCGAG CGGCTCAGCT GTTTCTCTGC 
TCTCTGGGCC TGAAGGTGCT ACCACCGGAG AAGCAGAGTT CGGGAGGCTC TGCCGAGCTC 
CAGTACGGCT GGCTGAAGAT CCCACCACGC TGCATGTTCG AACATCCTGA CGCTGAACGC 
ACGCTCAACC ACCTCATCTC TGGCTTCCTG AACTTCGAGA GCAAGATCAA CTACACGTTT 
AAGAACAAGG CGTACCTGCT GCAGGCCTTC ACACACGCCT CGTATCATTA CAACACCATT 
ACAGATTGTT ATCAGCGGTT GGAGTTTCTT GGTGATGCAA TTCTAGACTA CCTCATAACC 
AAGCACCTTT ATGAAGACCC CCGGCAGCAC TCGCCCGGCG TGCTGACCGA CCTGCGCTCT 
GCACTTGTCA ACAACACCAT ATTCGCTTCC CTGGCTGTCA AGTATGATTA CCACAAGTAC 
TTCAAAGCTG TCTCGCCAGA ACTCTTCCAC GTGATCGACG ATTTTGTGCA ATTTCAACTG 
GAGAAGAATG AAATGCAAGG AATGGATTCA GAGCTTCGCC GATCGGAGGA AGATGAAGAG 
AAAGAGGAAG ACATTGAAGT CCCCAAAGCA ATGGGAGACA TCTTTGAATC GCTAGCTGGT 
GCAATTTACA TGGATAGCGG GATGTCACTT GAGACAGTTT GGCAAGTGTA TTATCCAATG 
ATGAGGCCGC TCATAGAAAA ATTCTCCGCT AATGTCCCTC GATCCCCAGT GCGTGAACTC 
CTGGAGATGG AGCCAGAGAC AGCTAAGTTC AGCCCTGCGG AAAGAACTTA TGATGGAAAG 
GTACGGGTGA CGGTGGAAGT CGTGGGCAAA GGCAAGTTCA AAGGCGTAGG ACGCAGCTAC 
CGGATAGCCA AGTCAGCAGC CGCCCGCCGA GCCCTGCGCA GTCTCAAAGC CAACCAACCT 
CAGGTCCAAA ACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001154925.1
CDS	184..5781
Translation	MAGLQLVTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALEHNTIVCLNTGSGKTFIAVLLIKELSHQIRGENGKRTVFLVNAASSVAQQASTVRTHSDLQVGDYMSEDMTSWPEEMWNREMIENQVLVMTCHIFLHVLKNGVLPLSKINLLVFDECHLAITGHPYREIMKICEGCPSCPRILGLTASILNGKCDPCDLEEKIQNLEKILQSNAETATDLVVLDRYASQPREEVLDCGQYQDQSGLSERLLNELDEALNFLNDCNLSVHREDRDPTFISKQVLNDCRAVLTVLGPWCADKAAGIMVRELQKYIKHEQEELNRKFLLFTDTILRKIHALCEEHFSPASLDLKFVTPKVIRLLEILHEYKPFERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEAEAKEKTEANFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHSIGKNQPRNKQMEVEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPVSNYIMLADSERTKTFQEDLKTYKAIEKILRNKCSKSAECNDFELEPVTDDDNVLPPYVLRSEDGGPRVTMNTAIGHVNRYCARLPSDPFTHLAPKCKTVEMNTGGYRSTLFLPINSPLRVPVTGPVMNCARLAEKAVALLCCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCSPKAIPECLRGCYPVPEQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPRIPHFPVYTRSGEVTISIELQKSGFSLSAEQLELITRLHQYIFSHILRLEKPALEFKPVEADSAYCVLPLNIVEDSNTLDLDFKFMEDIEKSEARIGIPNTQYTKQNPFIFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLSNVNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRSQTAIDAGVGAQTLPPDFRYPNLDFGWKKSIDSKSFISCPSACMEEDDDHCKLGTSSDSNHTAPESCSMEVSQPPEGAPNTPDEKLETLTLPVTDLNKDCFPNLPNGTQADSDDLPHRSDVCQCSQLGPLERDLSTQTTTSVSVRPSPAGEPQPWPSDECTGRSSDLCDPHVKKPTSKHCPKSETATSTPAPSETSSEDCRSACAGPAWDSPKTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSSTDKWDSDENKDLANGKASDDEDEDDDDEPEEAEVEPSKEDVNVEDDLEYYYEHIRFIDSMLIGSGAFGKKISLQPTDPGYEWKAPKKAHNSHFSPDGGADEFDYSSWDAMCYLDPSKAGEEDDFVVGFWNPSEENCGTDIGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPPEKQSSGGSAELQYGWLKIPPRCMFEHPDAERTLNHLISGFLNFESKINYTFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVQNN

Target ORF information:

RefSeq Version	NM_001161453.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio dicer 1, ribonuclease type III (dicer1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001161453.2

ORF Insert Sequence:

ATGGCTGGCC TACAGCTGGT GACCCCTGCC TCCTCACCCA TGGGGCCCTT CTTTGGTCTC 
CCGTGGCAAC AGGAAGCTAT CCATGACAAC ATATACACAC CAAGGAAATA TCAGGTTGAA 
CTTCTTGAAG CAGCTCTTGA ACATAATACT ATAGTCTGCT TAAATACTGG CTCAGGGAAG 
ACCTTTATCG CAGTGCTCCT AATCAAAGAA CTCTCCCACC AAATTCGTGG CGAGAATGGG 
AAGAGGACAG TTTTCCTGGT GAATGCAGCG TCATCTGTGG CTCAGCAAGC ATCTACTGTA 
AGAACACACT CTGATCTCCA AGTAGGCGAC TATATGTCAG AGGACATGAC ATCATGGCCC 
GAGGAGATGT GGAACAGAGA GATGATAGAG AATCAGGTGC TGGTGATGAC GTGCCACATC 
TTCCTGCATG TTTTAAAGAA TGGAGTCTTG CCACTATCAA AGATCAACCT GCTGGTTTTT 
GACGAATGCC ACCTTGCAAT CACTGGCCAT CCTTATCGGG AAATAATGAA GATTTGTGAA 
GGTTGCCCCA GCTGTCCTCG CATTTTGGGT CTCACTGCTT CCATTTTAAA TGGCAAATGT 
GACCCTTGTG ATTTGGAGGA GAAAATTCAG AACCTGGAGA AGATTCTTCA GAGCAATGCA 
GAAACTGCCA CTGATCTTGT GGTGCTGGAT AGGTATGCGT CGCAGCCGCG TGAGGAGGTG 
CTGGATTGTG GACAGTACCA AGATCAGAGT GGGCTCTCGG AGAGGCTTTT AAATGAGCTG 
GACGAAGCCC TCAATTTTCT AAACGATTGC AACTTGTCTG TACATAGAGA AGATAGAGAC 
CCCACGTTTA TCTCCAAACA GGTGCTGAAT GACTGTCGTG CTGTGTTGAC TGTGCTGGGC 
CCTTGGTGTG CGGATAAAGC TGCAGGAATC ATGGTTCGGG AGCTGCAGAA ATATATTAAA 
CATGAACAGG AAGAGCTAAA CCGCAAGTTC CTGTTGTTCA CCGACACCAT CCTGAGGAAA 
ATCCATGCCT TATGTGAGGA GCATTTTTCC CCAGCTTCCC TCGACCTGAA GTTTGTCACT 
CCCAAGGTCA TCAGACTTTT GGAGATTCTC CACGAGTACA AGCCTTTCGA ACGGCAGCAG 
TTTGAGAGTG TGGAGTGGTA CAACAATCGC AATCAGGACA ATTATGTTTC TTGGAGTGAT 
TCTGAAGATG ATGATGAGGA CGAAGAGGCG GAGGCAAAGG AGAAAACCGA AGCAAATTTT 
CCCTCGCCGT TCACAAATAT CCTCTGTGGA ATCATCTTTG TGGAAAGGCG ATATACAGCG 
GTTGTTTTAA ACAGACTTAT TAAGGAGGCA GGAAAGCAAG ACCCAGAGCT GGCTTACATC 
AGTAGCAACT TCATCACAGG GCACAGCATC GGCAAGAACC AGCCTCGCAA CAAGCAGATG 
GAGGTGGAGT TCAGAAAACA AGAGGAAGTC TTGAGGAAAT TCAGAGCCCA TGAAACCAAC 
TTGCTCATTG CAACCAGCAT TGTGGAGGAA GGCGTCGATA TCCCAAAGTG CAATTTGGTC 
GTTCGGTTTG ACCTGCCGAC AGAGTACCGG TCGTACGTGC AATCCAAAGG TCGAGCCCGT 
GCTCCAGTCT CCAACTACAT CATGCTAGCA GACAGCGAAC GGACTAAAAC CTTCCAAGAA 
GACCTCAAGA CCTACAAGGC CATAGAGAAG ATCCTCCGAA ATAAGTGCTC GAAGTCGGCT 
GAATGCAATG ATTTTGAGCT GGAGCCTGTG ACCGATGACG ACAATGTTCT CCCACCGTAC 
GTCCTGCGCT CAGAGGACGG CGGGCCTCGT GTCACCATGA ACACTGCCAT TGGGCACGTC 
AACCGATATT GTGCGCGCTT GCCCAGTGAT CCGTTCACTC ATCTCGCTCC GAAGTGTAAA 
ACTGTAGAGA TGAACACCGG AGGGTATCGA TCCACACTCT TCCTACCTAT CAACTCACCA 
CTCAGAGTTC CTGTCACCGG GCCAGTAATG AATTGTGCAA GACTGGCTGA GAAAGCTGTC 
GCTCTTCTCT GTTGTGAAAA ACTCCACAAA ATTGGTGAAC TGGATGATCA TCTGATGCCT 
GTGGGAAAGG AAACTGTGAA GTATGAGGAG GAGCTGGACC TGCATGATGA AGAGGAGACA 
AGCGTGCCAG GAAGACCAGG CTCCACTAAA CGGAGACAGT GCTCCCCAAA AGCCATACCA 
GAGTGTCTGC GGGGTTGTTA CCCTGTGCCA GAGCAGCCTT GCTACCTTTA CGTTATTGGG 
ATGGTCCTCA CCACCCCTCT ACCTGACGAA CTCAACTTCC GGCGGAGGAA GCTGTATCCA 
CCAGAGGACA CAACCCGATG TTTTGGCATT CTGACAGCTA AACCAATACC CCGGATCCCT 
CATTTCCCTG TGTACACTCG TTCTGGTGAG GTGACCATCT CCATTGAGCT GCAGAAGTCG 
GGCTTTAGTC TAAGTGCGGA GCAGCTGGAG CTGATCACAC GTCTGCATCA GTACATCTTC 
TCCCACATCC TCCGTCTGGA AAAGCCAGCA CTAGAGTTTA AGCCTGTAGA GGCAGATTCT 
GCCTACTGTG TTCTACCTCT CAACATCGTT GAGGATTCCA ATACTTTGGA TCTGGATTTC 
AAGTTCATGG AGGATATTGA AAAGTCTGAA GCACGCATTG GCATTCCAAA CACTCAGTAC 
ACCAAGCAAA ACCCATTCAT CTTCAAATTG GAGGACTACC AGGATGCTGT CATCATTCCA 
AGGTATCGTA ATTTTGACCA GCCGCATCGG TTCTACGTGG CTGACGTTTA CACAGACCTC 
ACCCCGCTCA GTAAATTCCC CTCTCCAGAG TACGAGACTT TTGCCGAATA CTATAAAACC 
AAGTACAACC TGGACCTGTC AAATGTTAAT CAGCCCCTAC TGGATGTGGA CCACACATCT 
TCAAGATTGA ATCTTTTAAC TCCCCGGCAC CTTAATCAAA AGGGGAAAGC CCTTCCTCTC 
AGCAGTGCGG AGAAGAGAAA GGCCAAGTGG GAAAGCCTAC AAAACAAACA GATTTTAGTT 
CCGGAGCTCT GTGCCATTCA CCCCATCCCA GCCTCTTTGT GGAGGAAAGC CGTCTGCCTT 
CCCAGCATTC TCTATAGACT TCACTGCCTT CTGACGGCCG AAGAGCTGCG GTCTCAGACA 
GCCATCGATG CTGGCGTAGG GGCTCAGACC TTGCCTCCTG ATTTCAGGTA TCCTAATTTG 
GATTTCGGAT GGAAGAAATC CATCGACAGC AAGTCTTTCA TCTCCTGCCC CAGTGCATGT 
ATGGAAGAAG ACGACGATCA CTGTAAACTC GGCACAAGCT CCGATTCAAA CCACACTGCC 
CCCGAGAGCT GCAGTATGGA GGTCTCCCAG CCTCCAGAAG GCGCTCCCAA CACACCAGAT 
GAAAAGCTAG AAACCCTCAC TCTCCCTGTG ACTGATCTAA ACAAAGACTG TTTCCCAAAC 
CTCCCAAACG GCACTCAGGC CGACAGCGAC GACCTCCCTC ACAGAAGTGA CGTTTGCCAA 
TGCTCTCAGC TGGGCCCTCT GGAGAGAGAC CTATCAACAC AAACCACTAC CTCAGTTTCA 
GTGCGGCCCT CTCCAGCTGG AGAACCACAG CCATGGCCCA GCGATGAATG TACAGGCAGG 
AGCTCGGATC TCTGCGACCC ACATGTGAAG AAACCTACCT CAAAGCATTG CCCTAAATCG 
GAGACGGCGA CTAGCACCCC AGCACCTTCA GAAACGTCAT CAGAGGACTG TAGATCAGCC 
TGCGCTGGTC CAGCCTGGGA TTCGCCCAAA ACCCTGGGCC CGAACCCCGG GCTCATCCTG 
CAGGCCCTGA CTCTCTCCAA CGCCAGTGAC GGCTTCAATC TGGAACGCCT GGAGATGCTG 
GGCGACTCCT TCCTGAAACA CGCCATCACC ACATACCTGT TCTGCACTTA CCCTGACGCC 
CACGAGGGCA GACTGTCCTA CATGAGAAGC AAGAAGGTCA GCAACTGTAA CCTGTATCGG 
CTGGGGAAGA AGAAGGGTCT TCCGAGTCGA ATGGTGGTGT CCATCTTTGA CCCGCCGGTA 
AACTGGCTTC CTCCAGGCTA CGTCGTGAAT CAGGACAAAA GCAGCACAGA CAAGTGGGAC 
TCAGATGAGA ATAAAGATTT AGCGAATGGA AAAGCCAGCG ATGACGAAGA CGAGGATGAC 
GATGATGAGC CGGAAGAAGC AGAGGTCGAG CCATCTAAAG AAGATGTAAA TGTTGAAGAC 
GACCTGGAGT ATTATTACGA ACACATCCGC TTCATCGACA GCATGTTAAT CGGCTCTGGC 
GCTTTCGGCA AGAAGATCTC CCTCCAGCCC ACAGACCCCG GCTACGAATG GAAAGCTCCT 
AAAAAAGCCC ACAACTCCCA CTTCTCGCCA GACGGGGGTG CTGACGAGTT CGATTACAGC 
TCCTGGGACG CCATGTGTTA CCTGGACCCC AGTAAAGCAG GTGAGGAGGA CGATTTCGTG 
GTGGGCTTCT GGAATCCCTC TGAGGAAAAC TGCGGCACGG ACATCGGGAA ACAGTCCATC 
TCTTATGACC TTCACACGGA GCAGTGCATC GCAGATAAGA GCATAGCGGA CTGTGTGGAG 
GCTCTGCTGG GCTGCTATCT GACCAGCTGT GGTGAGCGAG CGGCTCAGCT GTTTCTCTGC 
TCTCTGGGCC TGAAGGTGCT ACCACCGGAG AAGCAGAGTT CGGGAGGCTC TGCCGAGCTC 
CAGTACGGCT GGCTGAAGAT CCCACCACGC TGCATGTTCG AACATCCTGA CGCTGAACGC 
ACGCTCAACC ACCTCATCTC TGGCTTCCTG AACTTCGAGA GCAAGATCAA CTACACGTTT 
AAGAACAAGG CGTACCTGCT GCAGGCCTTC ACACACGCCT CGTATCATTA CAACACCATT 
ACAGATTGTT ATCAGCGGTT GGAGTTTCTT GGTGATGCAA TTCTAGACTA CCTCATAACC 
AAGCACCTTT ATGAAGACCC CCGGCAGCAC TCGCCCGGCG TGCTGACCGA CCTGCGCTCT 
GCACTTGTCA ACAACACCAT ATTCGCTTCC CTGGCTGTCA AGTATGATTA CCACAAGTAC 
TTCAAAGCTG TCTCGCCAGA ACTCTTCCAC GTGATCGACG ATTTTGTGCA ATTTCAACTG 
GAGAAGAATG AAATGCAAGG AATGGATTCA GAGCTTCGCC GATCGGAGGA AGATGAAGAG 
AAAGAGGAAG ACATTGAAGT CCCCAAAGCA ATGGGAGACA TCTTTGAATC GCTAGCTGGT 
GCAATTTACA TGGATAGCGG GATGTCACTT GAGACAGTTT GGCAAGTGTA TTATCCAATG 
ATGAGGCCGC TCATAGAAAA ATTCTCCGCT AATGTCCCTC GATCCCCAGT GCGTGAACTC 
CTGGAGATGG AGCCAGAGAC AGCTAAGTTC AGCCCTGCGG AAAGAACTTA TGATGGAAAG 
GTACGGGTGA CGGTGGAAGT CGTGGGCAAA GGCAAGTTCA AAGGCGTAGG ACGCAGCTAC 
CGGATAGCCA AGTCAGCAGC CGCCCGCCGA GCCCTGCGCA GTCTCAAAGC CAACCAACCT 
CAGGTCCAAA ACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dicer1 ( XM_005158665.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005158722.1 Danio rerio dicer 1, ribonuclease type III (dicer1), transcript variant X1, mRNA.

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CloneID	ODa03830
Clone ID Related Accession (Same CDS sequence)	XM_005158665.3 , XM_005158666.3 , XM_021467115.1 , XM_005158665.4 , XM_021467114.1 , XM_021467116.1 , NM_001161453.2 , XM_005158666.4
Accession Version	XM_005158665.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5598bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	endoribonuclease Dicer isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005158665.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGCC TACAGCTGGT GACCCCTGCC TCCTCACCCA TGGGGCCCTT CTTTGGTCTC 
CCGTGGCAAC AGGAAGCTAT CCATGACAAC ATATACACAC CAAGGAAATA TCAGGTTGAA 
CTTCTTGAAG CAGCTCTTGA ACATAATACT ATAGTCTGCT TAAATACTGG CTCAGGGAAG 
ACCTTTATCG CAGTGCTCCT AATCAAAGAA CTCTCCCACC AAATTCGTGG CGAGAATGGG 
AAGAGGACAG TTTTCCTGGT GAATGCAGCG TCATCTGTGG CTCAGCAAGC ATCTACTGTA 
AGAACACACT CTGATCTCCA AGTAGGCGAC TATATGTCAG AGGACATGAC ATCATGGCCC 
GAGGAGATGT GGAACAGAGA GATGATAGAG AATCAGGTGC TGGTGATGAC GTGCCACATC 
TTCCTGCATG TTTTAAAGAA TGGAGTCTTG CCACTATCAA AGATCAACCT GCTGGTTTTT 
GACGAATGCC ACCTTGCAAT CACTGGCCAT CCTTATCGGG AAATAATGAA GATTTGTGAA 
GGTTGCCCCA GCTGTCCTCG CATTTTGGGT CTCACTGCTT CCATTTTAAA TGGCAAATGT 
GACCCTTGTG ATTTGGAGGA GAAAATTCAG AACCTGGAGA AGATTCTTCA GAGCAATGCA 
GAAACTGCCA CTGATCTTGT GGTGCTGGAT AGGTATGCGT CGCAGCCGCG TGAGGAGGTG 
CTGGATTGTG GACAGTACCA AGATCAGAGT GGGCTCTCGG AGAGGCTTTT AAATGAGCTG 
GACGAAGCCC TCAATTTTCT AAACGATTGC AACTTGTCTG TACATAGAGA AGATAGAGAC 
CCCACGTTTA TCTCCAAACA GGTGCTGAAT GACTGTCGTG CTGTGTTGAC TGTGCTGGGC 
CCTTGGTGTG CGGATAAAGC TGCAGGAATC ATGGTTCGGG AGCTGCAGAA ATATATTAAA 
CATGAACAGG AAGAGCTAAA CCGCAAGTTC CTGTTGTTCA CCGACACCAT CCTGAGGAAA 
ATCCATGCCT TATGTGAGGA GCATTTTTCC CCAGCTTCCC TCGACCTGAA GTTTGTCACT 
CCCAAGGTCA TCAGACTTTT GGAGATTCTC CACGAGTACA AGCCTTTCGA ACGGCAGCAG 
TTTGAGAGTG TGGAGTGGTA CAACAATCGC AATCAGGACA ATTATGTTTC TTGGAGTGAT 
TCTGAAGATG ATGATGAGGA CGAAGAGGCG GAGGCAAAGG AGAAAACCGA AGCAAATTTT 
CCCTCGCCGT TCACAAATAT CCTCTGTGGA ATCATCTTTG TGGAAAGGCG ATATACAGCG 
GTTGTTTTAA ACAGACTTAT TAAGGAGGCA GGAAAGCAAG ACCCAGAGCT GGCTTACATC 
AGTAGCAACT TCATCACAGG GCACAGCATC GGCAAGAACC AGCCTCGCAA CAAGCAGATG 
GAGGTGGAGT TCAGAAAACA AGAGGAAGTC TTGAGGAAAT TCAGAGCCCA TGAAACCAAC 
TTGCTCATTG CAACCAGCAT TGTGGAGGAA GGCGTCGATA TCCCAAAGTG CAATTTGGTC 
GTTCGGTTTG ACCTGCCGAC AGAGTACCGG TCGTACGTGC AATCCAAAGG TCGAGCCCGT 
GCTCCAGTCT CCAACTACAT CATGCTAGCA GACAGCGAAC GGACTAAAAC CTTCCAAGAA 
GACCTCAAGA CCTACAAGGC CATAGAGAAG ATCCTCCGAA ATAAGTGCTC GAAGTCGGCT 
GAATGCAATG ATTTTGAGCT GGAGCCTGTG ACCGATGACG ACAATGTTCT CCCACCGTAC 
GTCCTGCGCT CAGAGGACGG CGGGCCTCGT GTCACCATGA ACACTGCCAT TGGGCACGTC 
AACCGATATT GTGCGCGCTT GCCCAGTGAT CCGTTCACTC ATCTCGCTCC GAAGTGTAAA 
ACTGTAGAGA TGAACACCGG AGGGTATCGA TCCACACTCT TCCTACCTAT CAACTCACCA 
CTCAGAGTTC CTGTCACCGG GCCAGTAATG AATTGTGCAA GACTGGCTGA GAAAGCTGTC 
GCTCTTCTCT GTTGTGAAAA ACTCCACAAA ATTGGTGAAC TGGATGATCA TCTGATGCCT 
GTGGGAAAGG AAACTGTGAA GTATGAGGAG GAGCTGGACC TGCATGATGA AGAGGAGACA 
AGCGTGCCAG GAAGACCAGG CTCCACTAAA CGGAGACAGT GCTCCCCAAA AGCCATACCA 
GAGTGTCTGC GGGGTTGTTA CCCTGTGCCA GAGCAGCCTT GCTACCTTTA CGTTATTGGG 
ATGGTCCTCA CCACCCCTCT ACCTGACGAA CTCAACTTCC GGCGGAGGAA GCTGTATCCA 
CCAGAGGACA CAACCCGATG TTTTGGCATT CTGACAGCTA AACCAATACC CCGGATCCCT 
CATTTCCCTG TGTACACTCG TTCTGGTGAG GTGACCATCT CCATTGAGCT GCAGAAGTCG 
GGCTTTAGTC TAAGTGCGGA GCAGCTGGAG CTGATCACAC GTCTGCATCA GTACATCTTC 
TCCCACATCC TCCGTCTGGA AAAGCCAGCA CTAGAGTTTA AGCCTGTAGA GGCAGATTCT 
GCCTACTGTG TTCTACCTCT CAACATCGTT GAGGATTCCA ATACTTTGGA TCTGGATTTC 
AAGTTCATGG AGGATATTGA AAAGTCTGAA GCACGCATTG GCATTCCAAA CACTCAGTAC 
ACCAAGCAAA ACCCATTCAT CTTCAAATTG GAGGACTACC AGGATGCTGT CATCATTCCA 
AGGTATCGTA ATTTTGACCA GCCGCATCGG TTCTACGTGG CTGACGTTTA CACAGACCTC 
ACCCCGCTCA GTAAATTCCC CTCTCCAGAG TACGAGACTT TTGCCGAATA CTATAAAACC 
AAGTACAACC TGGACCTGTC AAATGTTAAT CAGCCCCTAC TGGATGTGGA CCACACATCT 
TCAAGATTGA ATCTTTTAAC TCCCCGGCAC CTTAATCAAA AGGGGAAAGC CCTTCCTCTC 
AGCAGTGCGG AGAAGAGAAA GGCCAAGTGG GAAAGCCTAC AAAACAAACA GATTTTAGTT 
CCGGAGCTCT GTGCCATTCA CCCCATCCCA GCCTCTTTGT GGAGGAAAGC CGTCTGCCTT 
CCCAGCATTC TCTATAGACT TCACTGCCTT CTGACGGCCG AAGAGCTGCG GTCTCAGACA 
GCCATCGATG CTGGCGTAGG GGCTCAGACC TTGCCTCCTG ATTTCAGGTA TCCTAATTTG 
GATTTCGGAT GGAAGAAATC CATCGACAGC AAGTCTTTCA TCTCCTGCCC CAGTGCATGT 
ATGGAAGAAG ACGACGATCA CTGTAAACTC GGCACAAGCT CCGATTCAAA CCACACTGCC 
CCCGAGAGCT GCAGTATGGA GGTCTCCCAG CCTCCAGAAG GCGCTCCCAA CACACCAGAT 
GAAAAGCTAG AAACCCTCAC TCTCCCTGTG ACTGATCTAA ACAAAGACTG TTTCCCAAAC 
CTCCCAAACG GCACTCAGGC CGACAGCGAC GACCTCCCTC ACAGAAGTGA CGTTTGCCAA 
TGCTCTCAGC TGGGCCCTCT GGAGAGAGAC CTATCAACAC AAACCACTAC CTCAGTTTCA 
GTGCGGCCCT CTCCAGCTGG AGAACCACAG CCATGGCCCA GCGATGAATG TACAGGCAGG 
AGCTCGGATC TCTGCGACCC ACATGTGAAG AAACCTACCT CAAAGCATTG CCCTAAATCG 
GAGACGGCGA CTAGCACCCC AGCACCTTCA GAAACGTCAT CAGAGGACTG TAGATCAGCC 
TGCGCTGGTC CAGCCTGGGA TTCGCCCAAA ACCCTGGGCC CGAACCCCGG GCTCATCCTG 
CAGGCCCTGA CTCTCTCCAA CGCCAGTGAC GGCTTCAATC TGGAACGCCT GGAGATGCTG 
GGCGACTCCT TCCTGAAACA CGCCATCACC ACATACCTGT TCTGCACTTA CCCTGACGCC 
CACGAGGGCA GACTGTCCTA CATGAGAAGC AAGAAGGTCA GCAACTGTAA CCTGTATCGG 
CTGGGGAAGA AGAAGGGTCT TCCGAGTCGA ATGGTGGTGT CCATCTTTGA CCCGCCGGTA 
AACTGGCTTC CTCCAGGCTA CGTCGTGAAT CAGGACAAAA GCAGCACAGA CAAGTGGGAC 
TCAGATGAGA ATAAAGATTT AGCGAATGGA AAAGCCAGCG ATGACGAAGA CGAGGATGAC 
GATGATGAGC CGGAAGAAGC AGAGGTCGAG CCATCTAAAG AAGATGTAAA TGTTGAAGAC 
GACCTGGAGT ATTATTACGA ACACATCCGC TTCATCGACA GCATGTTAAT CGGCTCTGGC 
GCTTTCGGCA AGAAGATCTC CCTCCAGCCC ACAGACCCCG GCTACGAATG GAAAGCTCCT 
AAAAAAGCCC ACAACTCCCA CTTCTCGCCA GACGGGGGTG CTGACGAGTT CGATTACAGC 
TCCTGGGACG CCATGTGTTA CCTGGACCCC AGTAAAGCAG GTGAGGAGGA CGATTTCGTG 
GTGGGCTTCT GGAATCCCTC TGAGGAAAAC TGCGGCACGG ACATCGGGAA ACAGTCCATC 
TCTTATGACC TTCACACGGA GCAGTGCATC GCAGATAAGA GCATAGCGGA CTGTGTGGAG 
GCTCTGCTGG GCTGCTATCT GACCAGCTGT GGTGAGCGAG CGGCTCAGCT GTTTCTCTGC 
TCTCTGGGCC TGAAGGTGCT ACCACCGGAG AAGCAGAGTT CGGGAGGCTC TGCCGAGCTC 
CAGTACGGCT GGCTGAAGAT CCCACCACGC TGCATGTTCG AACATCCTGA CGCTGAACGC 
ACGCTCAACC ACCTCATCTC TGGCTTCCTG AACTTCGAGA GCAAGATCAA CTACACGTTT 
AAGAACAAGG CGTACCTGCT GCAGGCCTTC ACACACGCCT CGTATCATTA CAACACCATT 
ACAGATTGTT ATCAGCGGTT GGAGTTTCTT GGTGATGCAA TTCTAGACTA CCTCATAACC 
AAGCACCTTT ATGAAGACCC CCGGCAGCAC TCGCCCGGCG TGCTGACCGA CCTGCGCTCT 
GCACTTGTCA ACAACACCAT ATTCGCTTCC CTGGCTGTCA AGTATGATTA CCACAAGTAC 
TTCAAAGCTG TCTCGCCAGA ACTCTTCCAC GTGATCGACG ATTTTGTGCA ATTTCAACTG 
GAGAAGAATG AAATGCAAGG AATGGATTCA GAGCTTCGCC GATCGGAGGA AGATGAAGAG 
AAAGAGGAAG ACATTGAAGT CCCCAAAGCA ATGGGAGACA TCTTTGAATC GCTAGCTGGT 
GCAATTTACA TGGATAGCGG GATGTCACTT GAGACAGTTT GGCAAGTGTA TTATCCAATG 
ATGAGGCCGC TCATAGAAAA ATTCTCCGCT AATGTCCCTC GATCCCCAGT GCGTGAACTC 
CTGGAGATGG AGCCAGAGAC AGCTAAGTTC AGCCCTGCGG AAAGAACTTA TGATGGAAAG 
GTACGGGTGA CGGTGGAAGT CGTGGGCAAA GGCAAGTTCA AAGGCGTAGG ACGCAGCTAC 
CGGATAGCCA AGTCAGCAGC CGCCCGCCGA GCCCTGCGCA GTCTCAAAGC CAACCAACCT 
CAGGTCCAAA ACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158722.1
CDS	374..5971
Translation	MAGLQLVTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALEHNTIVCLNTGSGKTFIAVLLIKELSHQIRGENGKRTVFLVNAASSVAQQASTVRTHSDLQVGDYMSEDMTSWPEEMWNREMIENQVLVMTCHIFLHVLKNGVLPLSKINLLVFDECHLAITGHPYREIMKICEGCPSCPRILGLTASILNGKCDPCDLEEKIQNLEKILQSNAETATDLVVLDRYASQPREEVLDCGQYQDQSGLSERLLNELDEALNFLNDCNLSVHREDRDPTFISKQVLNDCRAVLTVLGPWCADKAAGIMVRELQKYIKHEQEELNRKFLLFTDTILRKIHALCEEHFSPASLDLKFVTPKVIRLLEILHEYKPFERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEAEAKEKTEANFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHSIGKNQPRNKQMEVEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPVSNYIMLADSERTKTFQEDLKTYKAIEKILRNKCSKSAECNDFELEPVTDDDNVLPPYVLRSEDGGPRVTMNTAIGHVNRYCARLPSDPFTHLAPKCKTVEMNTGGYRSTLFLPINSPLRVPVTGPVMNCARLAEKAVALLCCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCSPKAIPECLRGCYPVPEQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPRIPHFPVYTRSGEVTISIELQKSGFSLSAEQLELITRLHQYIFSHILRLEKPALEFKPVEADSAYCVLPLNIVEDSNTLDLDFKFMEDIEKSEARIGIPNTQYTKQNPFIFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLSNVNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRSQTAIDAGVGAQTLPPDFRYPNLDFGWKKSIDSKSFISCPSACMEEDDDHCKLGTSSDSNHTAPESCSMEVSQPPEGAPNTPDEKLETLTLPVTDLNKDCFPNLPNGTQADSDDLPHRSDVCQCSQLGPLERDLSTQTTTSVSVRPSPAGEPQPWPSDECTGRSSDLCDPHVKKPTSKHCPKSETATSTPAPSETSSEDCRSACAGPAWDSPKTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSSTDKWDSDENKDLANGKASDDEDEDDDDEPEEAEVEPSKEDVNVEDDLEYYYEHIRFIDSMLIGSGAFGKKISLQPTDPGYEWKAPKKAHNSHFSPDGGADEFDYSSWDAMCYLDPSKAGEEDDFVVGFWNPSEENCGTDIGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPPEKQSSGGSAELQYGWLKIPPRCMFEHPDAERTLNHLISGFLNFESKINYTFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVQNN

Target ORF information:

RefSeq Version	XM_005158665.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio dicer 1, ribonuclease type III (dicer1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158665.3

ORF Insert Sequence:

ATGGCTGGCC TACAGCTGGT GACCCCTGCC TCCTCACCCA TGGGGCCCTT CTTTGGTCTC 
CCGTGGCAAC AGGAAGCTAT CCATGACAAC ATATACACAC CAAGGAAATA TCAGGTTGAA 
CTTCTTGAAG CAGCTCTTGA ACATAATACT ATAGTCTGCT TAAATACTGG CTCAGGGAAG 
ACCTTTATCG CAGTGCTCCT AATCAAAGAA CTCTCCCACC AAATTCGTGG CGAGAATGGG 
AAGAGGACAG TTTTCCTGGT GAATGCAGCG TCATCTGTGG CTCAGCAAGC ATCTACTGTA 
AGAACACACT CTGATCTCCA AGTAGGCGAC TATATGTCAG AGGACATGAC ATCATGGCCC 
GAGGAGATGT GGAACAGAGA GATGATAGAG AATCAGGTGC TGGTGATGAC GTGCCACATC 
TTCCTGCATG TTTTAAAGAA TGGAGTCTTG CCACTATCAA AGATCAACCT GCTGGTTTTT 
GACGAATGCC ACCTTGCAAT CACTGGCCAT CCTTATCGGG AAATAATGAA GATTTGTGAA 
GGTTGCCCCA GCTGTCCTCG CATTTTGGGT CTCACTGCTT CCATTTTAAA TGGCAAATGT 
GACCCTTGTG ATTTGGAGGA GAAAATTCAG AACCTGGAGA AGATTCTTCA GAGCAATGCA 
GAAACTGCCA CTGATCTTGT GGTGCTGGAT AGGTATGCGT CGCAGCCGCG TGAGGAGGTG 
CTGGATTGTG GACAGTACCA AGATCAGAGT GGGCTCTCGG AGAGGCTTTT AAATGAGCTG 
GACGAAGCCC TCAATTTTCT AAACGATTGC AACTTGTCTG TACATAGAGA AGATAGAGAC 
CCCACGTTTA TCTCCAAACA GGTGCTGAAT GACTGTCGTG CTGTGTTGAC TGTGCTGGGC 
CCTTGGTGTG CGGATAAAGC TGCAGGAATC ATGGTTCGGG AGCTGCAGAA ATATATTAAA 
CATGAACAGG AAGAGCTAAA CCGCAAGTTC CTGTTGTTCA CCGACACCAT CCTGAGGAAA 
ATCCATGCCT TATGTGAGGA GCATTTTTCC CCAGCTTCCC TCGACCTGAA GTTTGTCACT 
CCCAAGGTCA TCAGACTTTT GGAGATTCTC CACGAGTACA AGCCTTTCGA ACGGCAGCAG 
TTTGAGAGTG TGGAGTGGTA CAACAATCGC AATCAGGACA ATTATGTTTC TTGGAGTGAT 
TCTGAAGATG ATGATGAGGA CGAAGAGGCG GAGGCAAAGG AGAAAACCGA AGCAAATTTT 
CCCTCGCCGT TCACAAATAT CCTCTGTGGA ATCATCTTTG TGGAAAGGCG ATATACAGCG 
GTTGTTTTAA ACAGACTTAT TAAGGAGGCA GGAAAGCAAG ACCCAGAGCT GGCTTACATC 
AGTAGCAACT TCATCACAGG GCACAGCATC GGCAAGAACC AGCCTCGCAA CAAGCAGATG 
GAGGTGGAGT TCAGAAAACA AGAGGAAGTC TTGAGGAAAT TCAGAGCCCA TGAAACCAAC 
TTGCTCATTG CAACCAGCAT TGTGGAGGAA GGCGTCGATA TCCCAAAGTG CAATTTGGTC 
GTTCGGTTTG ACCTGCCGAC AGAGTACCGG TCGTACGTGC AATCCAAAGG TCGAGCCCGT 
GCTCCAGTCT CCAACTACAT CATGCTAGCA GACAGCGAAC GGACTAAAAC CTTCCAAGAA 
GACCTCAAGA CCTACAAGGC CATAGAGAAG ATCCTCCGAA ATAAGTGCTC GAAGTCGGCT 
GAATGCAATG ATTTTGAGCT GGAGCCTGTG ACCGATGACG ACAATGTTCT CCCACCGTAC 
GTCCTGCGCT CAGAGGACGG CGGGCCTCGT GTCACCATGA ACACTGCCAT TGGGCACGTC 
AACCGATATT GTGCGCGCTT GCCCAGTGAT CCGTTCACTC ATCTCGCTCC GAAGTGTAAA 
ACTGTAGAGA TGAACACCGG AGGGTATCGA TCCACACTCT TCCTACCTAT CAACTCACCA 
CTCAGAGTTC CTGTCACCGG GCCAGTAATG AATTGTGCAA GACTGGCTGA GAAAGCTGTC 
GCTCTTCTCT GTTGTGAAAA ACTCCACAAA ATTGGTGAAC TGGATGATCA TCTGATGCCT 
GTGGGAAAGG AAACTGTGAA GTATGAGGAG GAGCTGGACC TGCATGATGA AGAGGAGACA 
AGCGTGCCAG GAAGACCAGG CTCCACTAAA CGGAGACAGT GCTCCCCAAA AGCCATACCA 
GAGTGTCTGC GGGGTTGTTA CCCTGTGCCA GAGCAGCCTT GCTACCTTTA CGTTATTGGG 
ATGGTCCTCA CCACCCCTCT ACCTGACGAA CTCAACTTCC GGCGGAGGAA GCTGTATCCA 
CCAGAGGACA CAACCCGATG TTTTGGCATT CTGACAGCTA AACCAATACC CCGGATCCCT 
CATTTCCCTG TGTACACTCG TTCTGGTGAG GTGACCATCT CCATTGAGCT GCAGAAGTCG 
GGCTTTAGTC TAAGTGCGGA GCAGCTGGAG CTGATCACAC GTCTGCATCA GTACATCTTC 
TCCCACATCC TCCGTCTGGA AAAGCCAGCA CTAGAGTTTA AGCCTGTAGA GGCAGATTCT 
GCCTACTGTG TTCTACCTCT CAACATCGTT GAGGATTCCA ATACTTTGGA TCTGGATTTC 
AAGTTCATGG AGGATATTGA AAAGTCTGAA GCACGCATTG GCATTCCAAA CACTCAGTAC 
ACCAAGCAAA ACCCATTCAT CTTCAAATTG GAGGACTACC AGGATGCTGT CATCATTCCA 
AGGTATCGTA ATTTTGACCA GCCGCATCGG TTCTACGTGG CTGACGTTTA CACAGACCTC 
ACCCCGCTCA GTAAATTCCC CTCTCCAGAG TACGAGACTT TTGCCGAATA CTATAAAACC 
AAGTACAACC TGGACCTGTC AAATGTTAAT CAGCCCCTAC TGGATGTGGA CCACACATCT 
TCAAGATTGA ATCTTTTAAC TCCCCGGCAC CTTAATCAAA AGGGGAAAGC CCTTCCTCTC 
AGCAGTGCGG AGAAGAGAAA GGCCAAGTGG GAAAGCCTAC AAAACAAACA GATTTTAGTT 
CCGGAGCTCT GTGCCATTCA CCCCATCCCA GCCTCTTTGT GGAGGAAAGC CGTCTGCCTT 
CCCAGCATTC TCTATAGACT TCACTGCCTT CTGACGGCCG AAGAGCTGCG GTCTCAGACA 
GCCATCGATG CTGGCGTAGG GGCTCAGACC TTGCCTCCTG ATTTCAGGTA TCCTAATTTG 
GATTTCGGAT GGAAGAAATC CATCGACAGC AAGTCTTTCA TCTCCTGCCC CAGTGCATGT 
ATGGAAGAAG ACGACGATCA CTGTAAACTC GGCACAAGCT CCGATTCAAA CCACACTGCC 
CCCGAGAGCT GCAGTATGGA GGTCTCCCAG CCTCCAGAAG GCGCTCCCAA CACACCAGAT 
GAAAAGCTAG AAACCCTCAC TCTCCCTGTG ACTGATCTAA ACAAAGACTG TTTCCCAAAC 
CTCCCAAACG GCACTCAGGC CGACAGCGAC GACCTCCCTC ACAGAAGTGA CGTTTGCCAA 
TGCTCTCAGC TGGGCCCTCT GGAGAGAGAC CTATCAACAC AAACCACTAC CTCAGTTTCA 
GTGCGGCCCT CTCCAGCTGG AGAACCACAG CCATGGCCCA GCGATGAATG TACAGGCAGG 
AGCTCGGATC TCTGCGACCC ACATGTGAAG AAACCTACCT CAAAGCATTG CCCTAAATCG 
GAGACGGCGA CTAGCACCCC AGCACCTTCA GAAACGTCAT CAGAGGACTG TAGATCAGCC 
TGCGCTGGTC CAGCCTGGGA TTCGCCCAAA ACCCTGGGCC CGAACCCCGG GCTCATCCTG 
CAGGCCCTGA CTCTCTCCAA CGCCAGTGAC GGCTTCAATC TGGAACGCCT GGAGATGCTG 
GGCGACTCCT TCCTGAAACA CGCCATCACC ACATACCTGT TCTGCACTTA CCCTGACGCC 
CACGAGGGCA GACTGTCCTA CATGAGAAGC AAGAAGGTCA GCAACTGTAA CCTGTATCGG 
CTGGGGAAGA AGAAGGGTCT TCCGAGTCGA ATGGTGGTGT CCATCTTTGA CCCGCCGGTA 
AACTGGCTTC CTCCAGGCTA CGTCGTGAAT CAGGACAAAA GCAGCACAGA CAAGTGGGAC 
TCAGATGAGA ATAAAGATTT AGCGAATGGA AAAGCCAGCG ATGACGAAGA CGAGGATGAC 
GATGATGAGC CGGAAGAAGC AGAGGTCGAG CCATCTAAAG AAGATGTAAA TGTTGAAGAC 
GACCTGGAGT ATTATTACGA ACACATCCGC TTCATCGACA GCATGTTAAT CGGCTCTGGC 
GCTTTCGGCA AGAAGATCTC CCTCCAGCCC ACAGACCCCG GCTACGAATG GAAAGCTCCT 
AAAAAAGCCC ACAACTCCCA CTTCTCGCCA GACGGGGGTG CTGACGAGTT CGATTACAGC 
TCCTGGGACG CCATGTGTTA CCTGGACCCC AGTAAAGCAG GTGAGGAGGA CGATTTCGTG 
GTGGGCTTCT GGAATCCCTC TGAGGAAAAC TGCGGCACGG ACATCGGGAA ACAGTCCATC 
TCTTATGACC TTCACACGGA GCAGTGCATC GCAGATAAGA GCATAGCGGA CTGTGTGGAG 
GCTCTGCTGG GCTGCTATCT GACCAGCTGT GGTGAGCGAG CGGCTCAGCT GTTTCTCTGC 
TCTCTGGGCC TGAAGGTGCT ACCACCGGAG AAGCAGAGTT CGGGAGGCTC TGCCGAGCTC 
CAGTACGGCT GGCTGAAGAT CCCACCACGC TGCATGTTCG AACATCCTGA CGCTGAACGC 
ACGCTCAACC ACCTCATCTC TGGCTTCCTG AACTTCGAGA GCAAGATCAA CTACACGTTT 
AAGAACAAGG CGTACCTGCT GCAGGCCTTC ACACACGCCT CGTATCATTA CAACACCATT 
ACAGATTGTT ATCAGCGGTT GGAGTTTCTT GGTGATGCAA TTCTAGACTA CCTCATAACC 
AAGCACCTTT ATGAAGACCC CCGGCAGCAC TCGCCCGGCG TGCTGACCGA CCTGCGCTCT 
GCACTTGTCA ACAACACCAT ATTCGCTTCC CTGGCTGTCA AGTATGATTA CCACAAGTAC 
TTCAAAGCTG TCTCGCCAGA ACTCTTCCAC GTGATCGACG ATTTTGTGCA ATTTCAACTG 
GAGAAGAATG AAATGCAAGG AATGGATTCA GAGCTTCGCC GATCGGAGGA AGATGAAGAG 
AAAGAGGAAG ACATTGAAGT CCCCAAAGCA ATGGGAGACA TCTTTGAATC GCTAGCTGGT 
GCAATTTACA TGGATAGCGG GATGTCACTT GAGACAGTTT GGCAAGTGTA TTATCCAATG 
ATGAGGCCGC TCATAGAAAA ATTCTCCGCT AATGTCCCTC GATCCCCAGT GCGTGAACTC 
CTGGAGATGG AGCCAGAGAC AGCTAAGTTC AGCCCTGCGG AAAGAACTTA TGATGGAAAG 
GTACGGGTGA CGGTGGAAGT CGTGGGCAAA GGCAAGTTCA AAGGCGTAGG ACGCAGCTAC 
CGGATAGCCA AGTCAGCAGC CGCCCGCCGA GCCCTGCGCA GTCTCAAAGC CAACCAACCT 
CAGGTCCAAA ACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dicer1 ( XM_005158666.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005158723.1 Danio rerio dicer 1, ribonuclease type III (dicer1), transcript variant X2, mRNA.

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CloneID	ODa03830
Clone ID Related Accession (Same CDS sequence)	XM_005158665.3 , XM_005158666.3 , XM_021467115.1 , XM_005158665.4 , XM_021467114.1 , XM_021467116.1 , NM_001161453.2 , XM_005158666.4
Accession Version	XM_005158666.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5598bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	endoribonuclease Dicer isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005158666.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGCC TACAGCTGGT GACCCCTGCC TCCTCACCCA TGGGGCCCTT CTTTGGTCTC 
CCGTGGCAAC AGGAAGCTAT CCATGACAAC ATATACACAC CAAGGAAATA TCAGGTTGAA 
CTTCTTGAAG CAGCTCTTGA ACATAATACT ATAGTCTGCT TAAATACTGG CTCAGGGAAG 
ACCTTTATCG CAGTGCTCCT AATCAAAGAA CTCTCCCACC AAATTCGTGG CGAGAATGGG 
AAGAGGACAG TTTTCCTGGT GAATGCAGCG TCATCTGTGG CTCAGCAAGC ATCTACTGTA 
AGAACACACT CTGATCTCCA AGTAGGCGAC TATATGTCAG AGGACATGAC ATCATGGCCC 
GAGGAGATGT GGAACAGAGA GATGATAGAG AATCAGGTGC TGGTGATGAC GTGCCACATC 
TTCCTGCATG TTTTAAAGAA TGGAGTCTTG CCACTATCAA AGATCAACCT GCTGGTTTTT 
GACGAATGCC ACCTTGCAAT CACTGGCCAT CCTTATCGGG AAATAATGAA GATTTGTGAA 
GGTTGCCCCA GCTGTCCTCG CATTTTGGGT CTCACTGCTT CCATTTTAAA TGGCAAATGT 
GACCCTTGTG ATTTGGAGGA GAAAATTCAG AACCTGGAGA AGATTCTTCA GAGCAATGCA 
GAAACTGCCA CTGATCTTGT GGTGCTGGAT AGGTATGCGT CGCAGCCGCG TGAGGAGGTG 
CTGGATTGTG GACAGTACCA AGATCAGAGT GGGCTCTCGG AGAGGCTTTT AAATGAGCTG 
GACGAAGCCC TCAATTTTCT AAACGATTGC AACTTGTCTG TACATAGAGA AGATAGAGAC 
CCCACGTTTA TCTCCAAACA GGTGCTGAAT GACTGTCGTG CTGTGTTGAC TGTGCTGGGC 
CCTTGGTGTG CGGATAAAGC TGCAGGAATC ATGGTTCGGG AGCTGCAGAA ATATATTAAA 
CATGAACAGG AAGAGCTAAA CCGCAAGTTC CTGTTGTTCA CCGACACCAT CCTGAGGAAA 
ATCCATGCCT TATGTGAGGA GCATTTTTCC CCAGCTTCCC TCGACCTGAA GTTTGTCACT 
CCCAAGGTCA TCAGACTTTT GGAGATTCTC CACGAGTACA AGCCTTTCGA ACGGCAGCAG 
TTTGAGAGTG TGGAGTGGTA CAACAATCGC AATCAGGACA ATTATGTTTC TTGGAGTGAT 
TCTGAAGATG ATGATGAGGA CGAAGAGGCG GAGGCAAAGG AGAAAACCGA AGCAAATTTT 
CCCTCGCCGT TCACAAATAT CCTCTGTGGA ATCATCTTTG TGGAAAGGCG ATATACAGCG 
GTTGTTTTAA ACAGACTTAT TAAGGAGGCA GGAAAGCAAG ACCCAGAGCT GGCTTACATC 
AGTAGCAACT TCATCACAGG GCACAGCATC GGCAAGAACC AGCCTCGCAA CAAGCAGATG 
GAGGTGGAGT TCAGAAAACA AGAGGAAGTC TTGAGGAAAT TCAGAGCCCA TGAAACCAAC 
TTGCTCATTG CAACCAGCAT TGTGGAGGAA GGCGTCGATA TCCCAAAGTG CAATTTGGTC 
GTTCGGTTTG ACCTGCCGAC AGAGTACCGG TCGTACGTGC AATCCAAAGG TCGAGCCCGT 
GCTCCAGTCT CCAACTACAT CATGCTAGCA GACAGCGAAC GGACTAAAAC CTTCCAAGAA 
GACCTCAAGA CCTACAAGGC CATAGAGAAG ATCCTCCGAA ATAAGTGCTC GAAGTCGGCT 
GAATGCAATG ATTTTGAGCT GGAGCCTGTG ACCGATGACG ACAATGTTCT CCCACCGTAC 
GTCCTGCGCT CAGAGGACGG CGGGCCTCGT GTCACCATGA ACACTGCCAT TGGGCACGTC 
AACCGATATT GTGCGCGCTT GCCCAGTGAT CCGTTCACTC ATCTCGCTCC GAAGTGTAAA 
ACTGTAGAGA TGAACACCGG AGGGTATCGA TCCACACTCT TCCTACCTAT CAACTCACCA 
CTCAGAGTTC CTGTCACCGG GCCAGTAATG AATTGTGCAA GACTGGCTGA GAAAGCTGTC 
GCTCTTCTCT GTTGTGAAAA ACTCCACAAA ATTGGTGAAC TGGATGATCA TCTGATGCCT 
GTGGGAAAGG AAACTGTGAA GTATGAGGAG GAGCTGGACC TGCATGATGA AGAGGAGACA 
AGCGTGCCAG GAAGACCAGG CTCCACTAAA CGGAGACAGT GCTCCCCAAA AGCCATACCA 
GAGTGTCTGC GGGGTTGTTA CCCTGTGCCA GAGCAGCCTT GCTACCTTTA CGTTATTGGG 
ATGGTCCTCA CCACCCCTCT ACCTGACGAA CTCAACTTCC GGCGGAGGAA GCTGTATCCA 
CCAGAGGACA CAACCCGATG TTTTGGCATT CTGACAGCTA AACCAATACC CCGGATCCCT 
CATTTCCCTG TGTACACTCG TTCTGGTGAG GTGACCATCT CCATTGAGCT GCAGAAGTCG 
GGCTTTAGTC TAAGTGCGGA GCAGCTGGAG CTGATCACAC GTCTGCATCA GTACATCTTC 
TCCCACATCC TCCGTCTGGA AAAGCCAGCA CTAGAGTTTA AGCCTGTAGA GGCAGATTCT 
GCCTACTGTG TTCTACCTCT CAACATCGTT GAGGATTCCA ATACTTTGGA TCTGGATTTC 
AAGTTCATGG AGGATATTGA AAAGTCTGAA GCACGCATTG GCATTCCAAA CACTCAGTAC 
ACCAAGCAAA ACCCATTCAT CTTCAAATTG GAGGACTACC AGGATGCTGT CATCATTCCA 
AGGTATCGTA ATTTTGACCA GCCGCATCGG TTCTACGTGG CTGACGTTTA CACAGACCTC 
ACCCCGCTCA GTAAATTCCC CTCTCCAGAG TACGAGACTT TTGCCGAATA CTATAAAACC 
AAGTACAACC TGGACCTGTC AAATGTTAAT CAGCCCCTAC TGGATGTGGA CCACACATCT 
TCAAGATTGA ATCTTTTAAC TCCCCGGCAC CTTAATCAAA AGGGGAAAGC CCTTCCTCTC 
AGCAGTGCGG AGAAGAGAAA GGCCAAGTGG GAAAGCCTAC AAAACAAACA GATTTTAGTT 
CCGGAGCTCT GTGCCATTCA CCCCATCCCA GCCTCTTTGT GGAGGAAAGC CGTCTGCCTT 
CCCAGCATTC TCTATAGACT TCACTGCCTT CTGACGGCCG AAGAGCTGCG GTCTCAGACA 
GCCATCGATG CTGGCGTAGG GGCTCAGACC TTGCCTCCTG ATTTCAGGTA TCCTAATTTG 
GATTTCGGAT GGAAGAAATC CATCGACAGC AAGTCTTTCA TCTCCTGCCC CAGTGCATGT 
ATGGAAGAAG ACGACGATCA CTGTAAACTC GGCACAAGCT CCGATTCAAA CCACACTGCC 
CCCGAGAGCT GCAGTATGGA GGTCTCCCAG CCTCCAGAAG GCGCTCCCAA CACACCAGAT 
GAAAAGCTAG AAACCCTCAC TCTCCCTGTG ACTGATCTAA ACAAAGACTG TTTCCCAAAC 
CTCCCAAACG GCACTCAGGC CGACAGCGAC GACCTCCCTC ACAGAAGTGA CGTTTGCCAA 
TGCTCTCAGC TGGGCCCTCT GGAGAGAGAC CTATCAACAC AAACCACTAC CTCAGTTTCA 
GTGCGGCCCT CTCCAGCTGG AGAACCACAG CCATGGCCCA GCGATGAATG TACAGGCAGG 
AGCTCGGATC TCTGCGACCC ACATGTGAAG AAACCTACCT CAAAGCATTG CCCTAAATCG 
GAGACGGCGA CTAGCACCCC AGCACCTTCA GAAACGTCAT CAGAGGACTG TAGATCAGCC 
TGCGCTGGTC CAGCCTGGGA TTCGCCCAAA ACCCTGGGCC CGAACCCCGG GCTCATCCTG 
CAGGCCCTGA CTCTCTCCAA CGCCAGTGAC GGCTTCAATC TGGAACGCCT GGAGATGCTG 
GGCGACTCCT TCCTGAAACA CGCCATCACC ACATACCTGT TCTGCACTTA CCCTGACGCC 
CACGAGGGCA GACTGTCCTA CATGAGAAGC AAGAAGGTCA GCAACTGTAA CCTGTATCGG 
CTGGGGAAGA AGAAGGGTCT TCCGAGTCGA ATGGTGGTGT CCATCTTTGA CCCGCCGGTA 
AACTGGCTTC CTCCAGGCTA CGTCGTGAAT CAGGACAAAA GCAGCACAGA CAAGTGGGAC 
TCAGATGAGA ATAAAGATTT AGCGAATGGA AAAGCCAGCG ATGACGAAGA CGAGGATGAC 
GATGATGAGC CGGAAGAAGC AGAGGTCGAG CCATCTAAAG AAGATGTAAA TGTTGAAGAC 
GACCTGGAGT ATTATTACGA ACACATCCGC TTCATCGACA GCATGTTAAT CGGCTCTGGC 
GCTTTCGGCA AGAAGATCTC CCTCCAGCCC ACAGACCCCG GCTACGAATG GAAAGCTCCT 
AAAAAAGCCC ACAACTCCCA CTTCTCGCCA GACGGGGGTG CTGACGAGTT CGATTACAGC 
TCCTGGGACG CCATGTGTTA CCTGGACCCC AGTAAAGCAG GTGAGGAGGA CGATTTCGTG 
GTGGGCTTCT GGAATCCCTC TGAGGAAAAC TGCGGCACGG ACATCGGGAA ACAGTCCATC 
TCTTATGACC TTCACACGGA GCAGTGCATC GCAGATAAGA GCATAGCGGA CTGTGTGGAG 
GCTCTGCTGG GCTGCTATCT GACCAGCTGT GGTGAGCGAG CGGCTCAGCT GTTTCTCTGC 
TCTCTGGGCC TGAAGGTGCT ACCACCGGAG AAGCAGAGTT CGGGAGGCTC TGCCGAGCTC 
CAGTACGGCT GGCTGAAGAT CCCACCACGC TGCATGTTCG AACATCCTGA CGCTGAACGC 
ACGCTCAACC ACCTCATCTC TGGCTTCCTG AACTTCGAGA GCAAGATCAA CTACACGTTT 
AAGAACAAGG CGTACCTGCT GCAGGCCTTC ACACACGCCT CGTATCATTA CAACACCATT 
ACAGATTGTT ATCAGCGGTT GGAGTTTCTT GGTGATGCAA TTCTAGACTA CCTCATAACC 
AAGCACCTTT ATGAAGACCC CCGGCAGCAC TCGCCCGGCG TGCTGACCGA CCTGCGCTCT 
GCACTTGTCA ACAACACCAT ATTCGCTTCC CTGGCTGTCA AGTATGATTA CCACAAGTAC 
TTCAAAGCTG TCTCGCCAGA ACTCTTCCAC GTGATCGACG ATTTTGTGCA ATTTCAACTG 
GAGAAGAATG AAATGCAAGG AATGGATTCA GAGCTTCGCC GATCGGAGGA AGATGAAGAG 
AAAGAGGAAG ACATTGAAGT CCCCAAAGCA ATGGGAGACA TCTTTGAATC GCTAGCTGGT 
GCAATTTACA TGGATAGCGG GATGTCACTT GAGACAGTTT GGCAAGTGTA TTATCCAATG 
ATGAGGCCGC TCATAGAAAA ATTCTCCGCT AATGTCCCTC GATCCCCAGT GCGTGAACTC 
CTGGAGATGG AGCCAGAGAC AGCTAAGTTC AGCCCTGCGG AAAGAACTTA TGATGGAAAG 
GTACGGGTGA CGGTGGAAGT CGTGGGCAAA GGCAAGTTCA AAGGCGTAGG ACGCAGCTAC 
CGGATAGCCA AGTCAGCAGC CGCCCGCCGA GCCCTGCGCA GTCTCAAAGC CAACCAACCT 
CAGGTCCAAA ACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158723.1
CDS	377..5974
Translation	MAGLQLVTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALEHNTIVCLNTGSGKTFIAVLLIKELSHQIRGENGKRTVFLVNAASSVAQQASTVRTHSDLQVGDYMSEDMTSWPEEMWNREMIENQVLVMTCHIFLHVLKNGVLPLSKINLLVFDECHLAITGHPYREIMKICEGCPSCPRILGLTASILNGKCDPCDLEEKIQNLEKILQSNAETATDLVVLDRYASQPREEVLDCGQYQDQSGLSERLLNELDEALNFLNDCNLSVHREDRDPTFISKQVLNDCRAVLTVLGPWCADKAAGIMVRELQKYIKHEQEELNRKFLLFTDTILRKIHALCEEHFSPASLDLKFVTPKVIRLLEILHEYKPFERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEAEAKEKTEANFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHSIGKNQPRNKQMEVEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPVSNYIMLADSERTKTFQEDLKTYKAIEKILRNKCSKSAECNDFELEPVTDDDNVLPPYVLRSEDGGPRVTMNTAIGHVNRYCARLPSDPFTHLAPKCKTVEMNTGGYRSTLFLPINSPLRVPVTGPVMNCARLAEKAVALLCCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCSPKAIPECLRGCYPVPEQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPRIPHFPVYTRSGEVTISIELQKSGFSLSAEQLELITRLHQYIFSHILRLEKPALEFKPVEADSAYCVLPLNIVEDSNTLDLDFKFMEDIEKSEARIGIPNTQYTKQNPFIFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLSNVNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRSQTAIDAGVGAQTLPPDFRYPNLDFGWKKSIDSKSFISCPSACMEEDDDHCKLGTSSDSNHTAPESCSMEVSQPPEGAPNTPDEKLETLTLPVTDLNKDCFPNLPNGTQADSDDLPHRSDVCQCSQLGPLERDLSTQTTTSVSVRPSPAGEPQPWPSDECTGRSSDLCDPHVKKPTSKHCPKSETATSTPAPSETSSEDCRSACAGPAWDSPKTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSSTDKWDSDENKDLANGKASDDEDEDDDDEPEEAEVEPSKEDVNVEDDLEYYYEHIRFIDSMLIGSGAFGKKISLQPTDPGYEWKAPKKAHNSHFSPDGGADEFDYSSWDAMCYLDPSKAGEEDDFVVGFWNPSEENCGTDIGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPPEKQSSGGSAELQYGWLKIPPRCMFEHPDAERTLNHLISGFLNFESKINYTFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVQNN

Target ORF information:

RefSeq Version	XM_005158666.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio dicer 1, ribonuclease type III (dicer1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158666.3

ORF Insert Sequence:

ATGGCTGGCC TACAGCTGGT GACCCCTGCC TCCTCACCCA TGGGGCCCTT CTTTGGTCTC 
CCGTGGCAAC AGGAAGCTAT CCATGACAAC ATATACACAC CAAGGAAATA TCAGGTTGAA 
CTTCTTGAAG CAGCTCTTGA ACATAATACT ATAGTCTGCT TAAATACTGG CTCAGGGAAG 
ACCTTTATCG CAGTGCTCCT AATCAAAGAA CTCTCCCACC AAATTCGTGG CGAGAATGGG 
AAGAGGACAG TTTTCCTGGT GAATGCAGCG TCATCTGTGG CTCAGCAAGC ATCTACTGTA 
AGAACACACT CTGATCTCCA AGTAGGCGAC TATATGTCAG AGGACATGAC ATCATGGCCC 
GAGGAGATGT GGAACAGAGA GATGATAGAG AATCAGGTGC TGGTGATGAC GTGCCACATC 
TTCCTGCATG TTTTAAAGAA TGGAGTCTTG CCACTATCAA AGATCAACCT GCTGGTTTTT 
GACGAATGCC ACCTTGCAAT CACTGGCCAT CCTTATCGGG AAATAATGAA GATTTGTGAA 
GGTTGCCCCA GCTGTCCTCG CATTTTGGGT CTCACTGCTT CCATTTTAAA TGGCAAATGT 
GACCCTTGTG ATTTGGAGGA GAAAATTCAG AACCTGGAGA AGATTCTTCA GAGCAATGCA 
GAAACTGCCA CTGATCTTGT GGTGCTGGAT AGGTATGCGT CGCAGCCGCG TGAGGAGGTG 
CTGGATTGTG GACAGTACCA AGATCAGAGT GGGCTCTCGG AGAGGCTTTT AAATGAGCTG 
GACGAAGCCC TCAATTTTCT AAACGATTGC AACTTGTCTG TACATAGAGA AGATAGAGAC 
CCCACGTTTA TCTCCAAACA GGTGCTGAAT GACTGTCGTG CTGTGTTGAC TGTGCTGGGC 
CCTTGGTGTG CGGATAAAGC TGCAGGAATC ATGGTTCGGG AGCTGCAGAA ATATATTAAA 
CATGAACAGG AAGAGCTAAA CCGCAAGTTC CTGTTGTTCA CCGACACCAT CCTGAGGAAA 
ATCCATGCCT TATGTGAGGA GCATTTTTCC CCAGCTTCCC TCGACCTGAA GTTTGTCACT 
CCCAAGGTCA TCAGACTTTT GGAGATTCTC CACGAGTACA AGCCTTTCGA ACGGCAGCAG 
TTTGAGAGTG TGGAGTGGTA CAACAATCGC AATCAGGACA ATTATGTTTC TTGGAGTGAT 
TCTGAAGATG ATGATGAGGA CGAAGAGGCG GAGGCAAAGG AGAAAACCGA AGCAAATTTT 
CCCTCGCCGT TCACAAATAT CCTCTGTGGA ATCATCTTTG TGGAAAGGCG ATATACAGCG 
GTTGTTTTAA ACAGACTTAT TAAGGAGGCA GGAAAGCAAG ACCCAGAGCT GGCTTACATC 
AGTAGCAACT TCATCACAGG GCACAGCATC GGCAAGAACC AGCCTCGCAA CAAGCAGATG 
GAGGTGGAGT TCAGAAAACA AGAGGAAGTC TTGAGGAAAT TCAGAGCCCA TGAAACCAAC 
TTGCTCATTG CAACCAGCAT TGTGGAGGAA GGCGTCGATA TCCCAAAGTG CAATTTGGTC 
GTTCGGTTTG ACCTGCCGAC AGAGTACCGG TCGTACGTGC AATCCAAAGG TCGAGCCCGT 
GCTCCAGTCT CCAACTACAT CATGCTAGCA GACAGCGAAC GGACTAAAAC CTTCCAAGAA 
GACCTCAAGA CCTACAAGGC CATAGAGAAG ATCCTCCGAA ATAAGTGCTC GAAGTCGGCT 
GAATGCAATG ATTTTGAGCT GGAGCCTGTG ACCGATGACG ACAATGTTCT CCCACCGTAC 
GTCCTGCGCT CAGAGGACGG CGGGCCTCGT GTCACCATGA ACACTGCCAT TGGGCACGTC 
AACCGATATT GTGCGCGCTT GCCCAGTGAT CCGTTCACTC ATCTCGCTCC GAAGTGTAAA 
ACTGTAGAGA TGAACACCGG AGGGTATCGA TCCACACTCT TCCTACCTAT CAACTCACCA 
CTCAGAGTTC CTGTCACCGG GCCAGTAATG AATTGTGCAA GACTGGCTGA GAAAGCTGTC 
GCTCTTCTCT GTTGTGAAAA ACTCCACAAA ATTGGTGAAC TGGATGATCA TCTGATGCCT 
GTGGGAAAGG AAACTGTGAA GTATGAGGAG GAGCTGGACC TGCATGATGA AGAGGAGACA 
AGCGTGCCAG GAAGACCAGG CTCCACTAAA CGGAGACAGT GCTCCCCAAA AGCCATACCA 
GAGTGTCTGC GGGGTTGTTA CCCTGTGCCA GAGCAGCCTT GCTACCTTTA CGTTATTGGG 
ATGGTCCTCA CCACCCCTCT ACCTGACGAA CTCAACTTCC GGCGGAGGAA GCTGTATCCA 
CCAGAGGACA CAACCCGATG TTTTGGCATT CTGACAGCTA AACCAATACC CCGGATCCCT 
CATTTCCCTG TGTACACTCG TTCTGGTGAG GTGACCATCT CCATTGAGCT GCAGAAGTCG 
GGCTTTAGTC TAAGTGCGGA GCAGCTGGAG CTGATCACAC GTCTGCATCA GTACATCTTC 
TCCCACATCC TCCGTCTGGA AAAGCCAGCA CTAGAGTTTA AGCCTGTAGA GGCAGATTCT 
GCCTACTGTG TTCTACCTCT CAACATCGTT GAGGATTCCA ATACTTTGGA TCTGGATTTC 
AAGTTCATGG AGGATATTGA AAAGTCTGAA GCACGCATTG GCATTCCAAA CACTCAGTAC 
ACCAAGCAAA ACCCATTCAT CTTCAAATTG GAGGACTACC AGGATGCTGT CATCATTCCA 
AGGTATCGTA ATTTTGACCA GCCGCATCGG TTCTACGTGG CTGACGTTTA CACAGACCTC 
ACCCCGCTCA GTAAATTCCC CTCTCCAGAG TACGAGACTT TTGCCGAATA CTATAAAACC 
AAGTACAACC TGGACCTGTC AAATGTTAAT CAGCCCCTAC TGGATGTGGA CCACACATCT 
TCAAGATTGA ATCTTTTAAC TCCCCGGCAC CTTAATCAAA AGGGGAAAGC CCTTCCTCTC 
AGCAGTGCGG AGAAGAGAAA GGCCAAGTGG GAAAGCCTAC AAAACAAACA GATTTTAGTT 
CCGGAGCTCT GTGCCATTCA CCCCATCCCA GCCTCTTTGT GGAGGAAAGC CGTCTGCCTT 
CCCAGCATTC TCTATAGACT TCACTGCCTT CTGACGGCCG AAGAGCTGCG GTCTCAGACA 
GCCATCGATG CTGGCGTAGG GGCTCAGACC TTGCCTCCTG ATTTCAGGTA TCCTAATTTG 
GATTTCGGAT GGAAGAAATC CATCGACAGC AAGTCTTTCA TCTCCTGCCC CAGTGCATGT 
ATGGAAGAAG ACGACGATCA CTGTAAACTC GGCACAAGCT CCGATTCAAA CCACACTGCC 
CCCGAGAGCT GCAGTATGGA GGTCTCCCAG CCTCCAGAAG GCGCTCCCAA CACACCAGAT 
GAAAAGCTAG AAACCCTCAC TCTCCCTGTG ACTGATCTAA ACAAAGACTG TTTCCCAAAC 
CTCCCAAACG GCACTCAGGC CGACAGCGAC GACCTCCCTC ACAGAAGTGA CGTTTGCCAA 
TGCTCTCAGC TGGGCCCTCT GGAGAGAGAC CTATCAACAC AAACCACTAC CTCAGTTTCA 
GTGCGGCCCT CTCCAGCTGG AGAACCACAG CCATGGCCCA GCGATGAATG TACAGGCAGG 
AGCTCGGATC TCTGCGACCC ACATGTGAAG AAACCTACCT CAAAGCATTG CCCTAAATCG 
GAGACGGCGA CTAGCACCCC AGCACCTTCA GAAACGTCAT CAGAGGACTG TAGATCAGCC 
TGCGCTGGTC CAGCCTGGGA TTCGCCCAAA ACCCTGGGCC CGAACCCCGG GCTCATCCTG 
CAGGCCCTGA CTCTCTCCAA CGCCAGTGAC GGCTTCAATC TGGAACGCCT GGAGATGCTG 
GGCGACTCCT TCCTGAAACA CGCCATCACC ACATACCTGT TCTGCACTTA CCCTGACGCC 
CACGAGGGCA GACTGTCCTA CATGAGAAGC AAGAAGGTCA GCAACTGTAA CCTGTATCGG 
CTGGGGAAGA AGAAGGGTCT TCCGAGTCGA ATGGTGGTGT CCATCTTTGA CCCGCCGGTA 
AACTGGCTTC CTCCAGGCTA CGTCGTGAAT CAGGACAAAA GCAGCACAGA CAAGTGGGAC 
TCAGATGAGA ATAAAGATTT AGCGAATGGA AAAGCCAGCG ATGACGAAGA CGAGGATGAC 
GATGATGAGC CGGAAGAAGC AGAGGTCGAG CCATCTAAAG AAGATGTAAA TGTTGAAGAC 
GACCTGGAGT ATTATTACGA ACACATCCGC TTCATCGACA GCATGTTAAT CGGCTCTGGC 
GCTTTCGGCA AGAAGATCTC CCTCCAGCCC ACAGACCCCG GCTACGAATG GAAAGCTCCT 
AAAAAAGCCC ACAACTCCCA CTTCTCGCCA GACGGGGGTG CTGACGAGTT CGATTACAGC 
TCCTGGGACG CCATGTGTTA CCTGGACCCC AGTAAAGCAG GTGAGGAGGA CGATTTCGTG 
GTGGGCTTCT GGAATCCCTC TGAGGAAAAC TGCGGCACGG ACATCGGGAA ACAGTCCATC 
TCTTATGACC TTCACACGGA GCAGTGCATC GCAGATAAGA GCATAGCGGA CTGTGTGGAG 
GCTCTGCTGG GCTGCTATCT GACCAGCTGT GGTGAGCGAG CGGCTCAGCT GTTTCTCTGC 
TCTCTGGGCC TGAAGGTGCT ACCACCGGAG AAGCAGAGTT CGGGAGGCTC TGCCGAGCTC 
CAGTACGGCT GGCTGAAGAT CCCACCACGC TGCATGTTCG AACATCCTGA CGCTGAACGC 
ACGCTCAACC ACCTCATCTC TGGCTTCCTG AACTTCGAGA GCAAGATCAA CTACACGTTT 
AAGAACAAGG CGTACCTGCT GCAGGCCTTC ACACACGCCT CGTATCATTA CAACACCATT 
ACAGATTGTT ATCAGCGGTT GGAGTTTCTT GGTGATGCAA TTCTAGACTA CCTCATAACC 
AAGCACCTTT ATGAAGACCC CCGGCAGCAC TCGCCCGGCG TGCTGACCGA CCTGCGCTCT 
GCACTTGTCA ACAACACCAT ATTCGCTTCC CTGGCTGTCA AGTATGATTA CCACAAGTAC 
TTCAAAGCTG TCTCGCCAGA ACTCTTCCAC GTGATCGACG ATTTTGTGCA ATTTCAACTG 
GAGAAGAATG AAATGCAAGG AATGGATTCA GAGCTTCGCC GATCGGAGGA AGATGAAGAG 
AAAGAGGAAG ACATTGAAGT CCCCAAAGCA ATGGGAGACA TCTTTGAATC GCTAGCTGGT 
GCAATTTACA TGGATAGCGG GATGTCACTT GAGACAGTTT GGCAAGTGTA TTATCCAATG 
ATGAGGCCGC TCATAGAAAA ATTCTCCGCT AATGTCCCTC GATCCCCAGT GCGTGAACTC 
CTGGAGATGG AGCCAGAGAC AGCTAAGTTC AGCCCTGCGG AAAGAACTTA TGATGGAAAG 
GTACGGGTGA CGGTGGAAGT CGTGGGCAAA GGCAAGTTCA AAGGCGTAGG ACGCAGCTAC 
CGGATAGCCA AGTCAGCAGC CGCCCGCCGA GCCCTGCGCA GTCTCAAAGC CAACCAACCT 
CAGGTCCAAA ACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dicer1 ( XM_021467115.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021322790.1 Danio rerio dicer 1, ribonuclease type III (dicer1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa03830
Clone ID Related Accession (Same CDS sequence)	XM_005158665.3 , XM_005158666.3 , XM_021467115.1 , XM_005158665.4 , XM_021467114.1 , XM_021467116.1 , NM_001161453.2 , XM_005158666.4
Accession Version	XM_021467115.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5598bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGCC TACAGCTGGT GACCCCTGCC TCCTCACCCA TGGGGCCCTT CTTTGGTCTC 
CCGTGGCAAC AGGAAGCTAT CCATGACAAC ATATACACAC CAAGGAAATA TCAGGTTGAA 
CTTCTTGAAG CAGCTCTTGA ACATAATACT ATAGTCTGCT TAAATACTGG CTCAGGGAAG 
ACCTTTATCG CAGTGCTCCT AATCAAAGAA CTCTCCCACC AAATTCGTGG CGAGAATGGG 
AAGAGGACAG TTTTCCTGGT GAATGCAGCG TCATCTGTGG CTCAGCAAGC ATCTACTGTA 
AGAACACACT CTGATCTCCA AGTAGGCGAC TATATGTCAG AGGACATGAC ATCATGGCCC 
GAGGAGATGT GGAACAGAGA GATGATAGAG AATCAGGTGC TGGTGATGAC GTGCCACATC 
TTCCTGCATG TTTTAAAGAA TGGAGTCTTG CCACTATCAA AGATCAACCT GCTGGTTTTT 
GACGAATGCC ACCTTGCAAT CACTGGCCAT CCTTATCGGG AAATAATGAA GATTTGTGAA 
GGTTGCCCCA GCTGTCCTCG CATTTTGGGT CTCACTGCTT CCATTTTAAA TGGCAAATGT 
GACCCTTGTG ATTTGGAGGA GAAAATTCAG AACCTGGAGA AGATTCTTCA GAGCAATGCA 
GAAACTGCCA CTGATCTTGT GGTGCTGGAT AGGTATGCGT CGCAGCCGCG TGAGGAGGTG 
CTGGATTGTG GACAGTACCA AGATCAGAGT GGGCTCTCGG AGAGGCTTTT AAATGAGCTG 
GACGAAGCCC TCAATTTTCT AAACGATTGC AACTTGTCTG TACATAGAGA AGATAGAGAC 
CCCACGTTTA TCTCCAAACA GGTGCTGAAT GACTGTCGTG CTGTGTTGAC TGTGCTGGGC 
CCTTGGTGTG CGGATAAAGC TGCAGGAATC ATGGTTCGGG AGCTGCAGAA ATATATTAAA 
CATGAACAGG AAGAGCTAAA CCGCAAGTTC CTGTTGTTCA CCGACACCAT CCTGAGGAAA 
ATCCATGCCT TATGTGAGGA GCATTTTTCC CCAGCTTCCC TCGACCTGAA GTTTGTCACT 
CCCAAGGTCA TCAGACTTTT GGAGATTCTC CACGAGTACA AGCCTTTCGA ACGGCAGCAG 
TTTGAGAGTG TGGAGTGGTA CAACAATCGC AATCAGGACA ATTATGTTTC TTGGAGTGAT 
TCTGAAGATG ATGATGAGGA CGAAGAGGCG GAGGCAAAGG AGAAAACCGA AGCAAATTTT 
CCCTCGCCGT TCACAAATAT CCTCTGTGGA ATCATCTTTG TGGAAAGGCG ATATACAGCG 
GTTGTTTTAA ACAGACTTAT TAAGGAGGCA GGAAAGCAAG ACCCAGAGCT GGCTTACATC 
AGTAGCAACT TCATCACAGG GCACAGCATC GGCAAGAACC AGCCTCGCAA CAAGCAGATG 
GAGGTGGAGT TCAGAAAACA AGAGGAAGTC TTGAGGAAAT TCAGAGCCCA TGAAACCAAC 
TTGCTCATTG CAACCAGCAT TGTGGAGGAA GGCGTCGATA TCCCAAAGTG CAATTTGGTC 
GTTCGGTTTG ACCTGCCGAC AGAGTACCGG TCGTACGTGC AATCCAAAGG TCGAGCCCGT 
GCTCCAGTCT CCAACTACAT CATGCTAGCA GACAGCGAAC GGACTAAAAC CTTCCAAGAA 
GACCTCAAGA CCTACAAGGC CATAGAGAAG ATCCTCCGAA ATAAGTGCTC GAAGTCGGCT 
GAATGCAATG ATTTTGAGCT GGAGCCTGTG ACCGATGACG ACAATGTTCT CCCACCGTAC 
GTCCTGCGCT CAGAGGACGG CGGGCCTCGT GTCACCATGA ACACTGCCAT TGGGCACGTC 
AACCGATATT GTGCGCGCTT GCCCAGTGAT CCGTTCACTC ATCTCGCTCC GAAGTGTAAA 
ACTGTAGAGA TGAACACCGG AGGGTATCGA TCCACACTCT TCCTACCTAT CAACTCACCA 
CTCAGAGTTC CTGTCACCGG GCCAGTAATG AATTGTGCAA GACTGGCTGA GAAAGCTGTC 
GCTCTTCTCT GTTGTGAAAA ACTCCACAAA ATTGGTGAAC TGGATGATCA TCTGATGCCT 
GTGGGAAAGG AAACTGTGAA GTATGAGGAG GAGCTGGACC TGCATGATGA AGAGGAGACA 
AGCGTGCCAG GAAGACCAGG CTCCACTAAA CGGAGACAGT GCTCCCCAAA AGCCATACCA 
GAGTGTCTGC GGGGTTGTTA CCCTGTGCCA GAGCAGCCTT GCTACCTTTA CGTTATTGGG 
ATGGTCCTCA CCACCCCTCT ACCTGACGAA CTCAACTTCC GGCGGAGGAA GCTGTATCCA 
CCAGAGGACA CAACCCGATG TTTTGGCATT CTGACAGCTA AACCAATACC CCGGATCCCT 
CATTTCCCTG TGTACACTCG TTCTGGTGAG GTGACCATCT CCATTGAGCT GCAGAAGTCG 
GGCTTTAGTC TAAGTGCGGA GCAGCTGGAG CTGATCACAC GTCTGCATCA GTACATCTTC 
TCCCACATCC TCCGTCTGGA AAAGCCAGCA CTAGAGTTTA AGCCTGTAGA GGCAGATTCT 
GCCTACTGTG TTCTACCTCT CAACATCGTT GAGGATTCCA ATACTTTGGA TCTGGATTTC 
AAGTTCATGG AGGATATTGA AAAGTCTGAA GCACGCATTG GCATTCCAAA CACTCAGTAC 
ACCAAGCAAA ACCCATTCAT CTTCAAATTG GAGGACTACC AGGATGCTGT CATCATTCCA 
AGGTATCGTA ATTTTGACCA GCCGCATCGG TTCTACGTGG CTGACGTTTA CACAGACCTC 
ACCCCGCTCA GTAAATTCCC CTCTCCAGAG TACGAGACTT TTGCCGAATA CTATAAAACC 
AAGTACAACC TGGACCTGTC AAATGTTAAT CAGCCCCTAC TGGATGTGGA CCACACATCT 
TCAAGATTGA ATCTTTTAAC TCCCCGGCAC CTTAATCAAA AGGGGAAAGC CCTTCCTCTC 
AGCAGTGCGG AGAAGAGAAA GGCCAAGTGG GAAAGCCTAC AAAACAAACA GATTTTAGTT 
CCGGAGCTCT GTGCCATTCA CCCCATCCCA GCCTCTTTGT GGAGGAAAGC CGTCTGCCTT 
CCCAGCATTC TCTATAGACT TCACTGCCTT CTGACGGCCG AAGAGCTGCG GTCTCAGACA 
GCCATCGATG CTGGCGTAGG GGCTCAGACC TTGCCTCCTG ATTTCAGGTA TCCTAATTTG 
GATTTCGGAT GGAAGAAATC CATCGACAGC AAGTCTTTCA TCTCCTGCCC CAGTGCATGT 
ATGGAAGAAG ACGACGATCA CTGTAAACTC GGCACAAGCT CCGATTCAAA CCACACTGCC 
CCCGAGAGCT GCAGTATGGA GGTCTCCCAG CCTCCAGAAG GCGCTCCCAA CACACCAGAT 
GAAAAGCTAG AAACCCTCAC TCTCCCTGTG ACTGATCTAA ACAAAGACTG TTTCCCAAAC 
CTCCCAAACG GCACTCAGGC CGACAGCGAC GACCTCCCTC ACAGAAGTGA CGTTTGCCAA 
TGCTCTCAGC TGGGCCCTCT GGAGAGAGAC CTATCAACAC AAACCACTAC CTCAGTTTCA 
GTGCGGCCCT CTCCAGCTGG AGAACCACAG CCATGGCCCA GCGATGAATG TACAGGCAGG 
AGCTCGGATC TCTGCGACCC ACATGTGAAG AAACCTACCT CAAAGCATTG CCCTAAATCG 
GAGACGGCGA CTAGCACCCC AGCACCTTCA GAAACGTCAT CAGAGGACTG TAGATCAGCC 
TGCGCTGGTC CAGCCTGGGA TTCGCCCAAA ACCCTGGGCC CGAACCCCGG GCTCATCCTG 
CAGGCCCTGA CTCTCTCCAA CGCCAGTGAC GGCTTCAATC TGGAACGCCT GGAGATGCTG 
GGCGACTCCT TCCTGAAACA CGCCATCACC ACATACCTGT TCTGCACTTA CCCTGACGCC 
CACGAGGGCA GACTGTCCTA CATGAGAAGC AAGAAGGTCA GCAACTGTAA CCTGTATCGG 
CTGGGGAAGA AGAAGGGTCT TCCGAGTCGA ATGGTGGTGT CCATCTTTGA CCCGCCGGTA 
AACTGGCTTC CTCCAGGCTA CGTCGTGAAT CAGGACAAAA GCAGCACAGA CAAGTGGGAC 
TCAGATGAGA ATAAAGATTT AGCGAATGGA AAAGCCAGCG ATGACGAAGA CGAGGATGAC 
GATGATGAGC CGGAAGAAGC AGAGGTCGAG CCATCTAAAG AAGATGTAAA TGTTGAAGAC 
GACCTGGAGT ATTATTACGA ACACATCCGC TTCATCGACA GCATGTTAAT CGGCTCTGGC 
GCTTTCGGCA AGAAGATCTC CCTCCAGCCC ACAGACCCCG GCTACGAATG GAAAGCTCCT 
AAAAAAGCCC ACAACTCCCA CTTCTCGCCA GACGGGGGTG CTGACGAGTT CGATTACAGC 
TCCTGGGACG CCATGTGTTA CCTGGACCCC AGTAAAGCAG GTGAGGAGGA CGATTTCGTG 
GTGGGCTTCT GGAATCCCTC TGAGGAAAAC TGCGGCACGG ACATCGGGAA ACAGTCCATC 
TCTTATGACC TTCACACGGA GCAGTGCATC GCAGATAAGA GCATAGCGGA CTGTGTGGAG 
GCTCTGCTGG GCTGCTATCT GACCAGCTGT GGTGAGCGAG CGGCTCAGCT GTTTCTCTGC 
TCTCTGGGCC TGAAGGTGCT ACCACCGGAG AAGCAGAGTT CGGGAGGCTC TGCCGAGCTC 
CAGTACGGCT GGCTGAAGAT CCCACCACGC TGCATGTTCG AACATCCTGA CGCTGAACGC 
ACGCTCAACC ACCTCATCTC TGGCTTCCTG AACTTCGAGA GCAAGATCAA CTACACGTTT 
AAGAACAAGG CGTACCTGCT GCAGGCCTTC ACACACGCCT CGTATCATTA CAACACCATT 
ACAGATTGTT ATCAGCGGTT GGAGTTTCTT GGTGATGCAA TTCTAGACTA CCTCATAACC 
AAGCACCTTT ATGAAGACCC CCGGCAGCAC TCGCCCGGCG TGCTGACCGA CCTGCGCTCT 
GCACTTGTCA ACAACACCAT ATTCGCTTCC CTGGCTGTCA AGTATGATTA CCACAAGTAC 
TTCAAAGCTG TCTCGCCAGA ACTCTTCCAC GTGATCGACG ATTTTGTGCA ATTTCAACTG 
GAGAAGAATG AAATGCAAGG AATGGATTCA GAGCTTCGCC GATCGGAGGA AGATGAAGAG 
AAAGAGGAAG ACATTGAAGT CCCCAAAGCA ATGGGAGACA TCTTTGAATC GCTAGCTGGT 
GCAATTTACA TGGATAGCGG GATGTCACTT GAGACAGTTT GGCAAGTGTA TTATCCAATG 
ATGAGGCCGC TCATAGAAAA ATTCTCCGCT AATGTCCCTC GATCCCCAGT GCGTGAACTC 
CTGGAGATGG AGCCAGAGAC AGCTAAGTTC AGCCCTGCGG AAAGAACTTA TGATGGAAAG 
GTACGGGTGA CGGTGGAAGT CGTGGGCAAA GGCAAGTTCA AAGGCGTAGG ACGCAGCTAC 
CGGATAGCCA AGTCAGCAGC CGCCCGCCGA GCCCTGCGCA GTCTCAAAGC CAACCAACCT 
CAGGTCCAAA ACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021322790.1
CDS	458..6055
Translation	MAGLQLVTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALEHNTIVCLNTGSGKTFIAVLLIKELSHQIRGENGKRTVFLVNAASSVAQQASTVRTHSDLQVGDYMSEDMTSWPEEMWNREMIENQVLVMTCHIFLHVLKNGVLPLSKINLLVFDECHLAITGHPYREIMKICEGCPSCPRILGLTASILNGKCDPCDLEEKIQNLEKILQSNAETATDLVVLDRYASQPREEVLDCGQYQDQSGLSERLLNELDEALNFLNDCNLSVHREDRDPTFISKQVLNDCRAVLTVLGPWCADKAAGIMVRELQKYIKHEQEELNRKFLLFTDTILRKIHALCEEHFSPASLDLKFVTPKVIRLLEILHEYKPFERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEAEAKEKTEANFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHSIGKNQPRNKQMEVEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPVSNYIMLADSERTKTFQEDLKTYKAIEKILRNKCSKSAECNDFELEPVTDDDNVLPPYVLRSEDGGPRVTMNTAIGHVNRYCARLPSDPFTHLAPKCKTVEMNTGGYRSTLFLPINSPLRVPVTGPVMNCARLAEKAVALLCCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCSPKAIPECLRGCYPVPEQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPRIPHFPVYTRSGEVTISIELQKSGFSLSAEQLELITRLHQYIFSHILRLEKPALEFKPVEADSAYCVLPLNIVEDSNTLDLDFKFMEDIEKSEARIGIPNTQYTKQNPFIFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLSNVNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRSQTAIDAGVGAQTLPPDFRYPNLDFGWKKSIDSKSFISCPSACMEEDDDHCKLGTSSDSNHTAPESCSMEVSQPPEGAPNTPDEKLETLTLPVTDLNKDCFPNLPNGTQADSDDLPHRSDVCQCSQLGPLERDLSTQTTTSVSVRPSPAGEPQPWPSDECTGRSSDLCDPHVKKPTSKHCPKSETATSTPAPSETSSEDCRSACAGPAWDSPKTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSSTDKWDSDENKDLANGKASDDEDEDDDDEPEEAEVEPSKEDVNVEDDLEYYYEHIRFIDSMLIGSGAFGKKISLQPTDPGYEWKAPKKAHNSHFSPDGGADEFDYSSWDAMCYLDPSKAGEEDDFVVGFWNPSEENCGTDIGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPPEKQSSGGSAELQYGWLKIPPRCMFEHPDAERTLNHLISGFLNFESKINYTFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVQNN

Target ORF information:

RefSeq Version	XM_021467115.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio dicer 1, ribonuclease type III (dicer1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021467115.1

ORF Insert Sequence:

ATGGCTGGCC TACAGCTGGT GACCCCTGCC TCCTCACCCA TGGGGCCCTT CTTTGGTCTC 
CCGTGGCAAC AGGAAGCTAT CCATGACAAC ATATACACAC CAAGGAAATA TCAGGTTGAA 
CTTCTTGAAG CAGCTCTTGA ACATAATACT ATAGTCTGCT TAAATACTGG CTCAGGGAAG 
ACCTTTATCG CAGTGCTCCT AATCAAAGAA CTCTCCCACC AAATTCGTGG CGAGAATGGG 
AAGAGGACAG TTTTCCTGGT GAATGCAGCG TCATCTGTGG CTCAGCAAGC ATCTACTGTA 
AGAACACACT CTGATCTCCA AGTAGGCGAC TATATGTCAG AGGACATGAC ATCATGGCCC 
GAGGAGATGT GGAACAGAGA GATGATAGAG AATCAGGTGC TGGTGATGAC GTGCCACATC 
TTCCTGCATG TTTTAAAGAA TGGAGTCTTG CCACTATCAA AGATCAACCT GCTGGTTTTT 
GACGAATGCC ACCTTGCAAT CACTGGCCAT CCTTATCGGG AAATAATGAA GATTTGTGAA 
GGTTGCCCCA GCTGTCCTCG CATTTTGGGT CTCACTGCTT CCATTTTAAA TGGCAAATGT 
GACCCTTGTG ATTTGGAGGA GAAAATTCAG AACCTGGAGA AGATTCTTCA GAGCAATGCA 
GAAACTGCCA CTGATCTTGT GGTGCTGGAT AGGTATGCGT CGCAGCCGCG TGAGGAGGTG 
CTGGATTGTG GACAGTACCA AGATCAGAGT GGGCTCTCGG AGAGGCTTTT AAATGAGCTG 
GACGAAGCCC TCAATTTTCT AAACGATTGC AACTTGTCTG TACATAGAGA AGATAGAGAC 
CCCACGTTTA TCTCCAAACA GGTGCTGAAT GACTGTCGTG CTGTGTTGAC TGTGCTGGGC 
CCTTGGTGTG CGGATAAAGC TGCAGGAATC ATGGTTCGGG AGCTGCAGAA ATATATTAAA 
CATGAACAGG AAGAGCTAAA CCGCAAGTTC CTGTTGTTCA CCGACACCAT CCTGAGGAAA 
ATCCATGCCT TATGTGAGGA GCATTTTTCC CCAGCTTCCC TCGACCTGAA GTTTGTCACT 
CCCAAGGTCA TCAGACTTTT GGAGATTCTC CACGAGTACA AGCCTTTCGA ACGGCAGCAG 
TTTGAGAGTG TGGAGTGGTA CAACAATCGC AATCAGGACA ATTATGTTTC TTGGAGTGAT 
TCTGAAGATG ATGATGAGGA CGAAGAGGCG GAGGCAAAGG AGAAAACCGA AGCAAATTTT 
CCCTCGCCGT TCACAAATAT CCTCTGTGGA ATCATCTTTG TGGAAAGGCG ATATACAGCG 
GTTGTTTTAA ACAGACTTAT TAAGGAGGCA GGAAAGCAAG ACCCAGAGCT GGCTTACATC 
AGTAGCAACT TCATCACAGG GCACAGCATC GGCAAGAACC AGCCTCGCAA CAAGCAGATG 
GAGGTGGAGT TCAGAAAACA AGAGGAAGTC TTGAGGAAAT TCAGAGCCCA TGAAACCAAC 
TTGCTCATTG CAACCAGCAT TGTGGAGGAA GGCGTCGATA TCCCAAAGTG CAATTTGGTC 
GTTCGGTTTG ACCTGCCGAC AGAGTACCGG TCGTACGTGC AATCCAAAGG TCGAGCCCGT 
GCTCCAGTCT CCAACTACAT CATGCTAGCA GACAGCGAAC GGACTAAAAC CTTCCAAGAA 
GACCTCAAGA CCTACAAGGC CATAGAGAAG ATCCTCCGAA ATAAGTGCTC GAAGTCGGCT 
GAATGCAATG ATTTTGAGCT GGAGCCTGTG ACCGATGACG ACAATGTTCT CCCACCGTAC 
GTCCTGCGCT CAGAGGACGG CGGGCCTCGT GTCACCATGA ACACTGCCAT TGGGCACGTC 
AACCGATATT GTGCGCGCTT GCCCAGTGAT CCGTTCACTC ATCTCGCTCC GAAGTGTAAA 
ACTGTAGAGA TGAACACCGG AGGGTATCGA TCCACACTCT TCCTACCTAT CAACTCACCA 
CTCAGAGTTC CTGTCACCGG GCCAGTAATG AATTGTGCAA GACTGGCTGA GAAAGCTGTC 
GCTCTTCTCT GTTGTGAAAA ACTCCACAAA ATTGGTGAAC TGGATGATCA TCTGATGCCT 
GTGGGAAAGG AAACTGTGAA GTATGAGGAG GAGCTGGACC TGCATGATGA AGAGGAGACA 
AGCGTGCCAG GAAGACCAGG CTCCACTAAA CGGAGACAGT GCTCCCCAAA AGCCATACCA 
GAGTGTCTGC GGGGTTGTTA CCCTGTGCCA GAGCAGCCTT GCTACCTTTA CGTTATTGGG 
ATGGTCCTCA CCACCCCTCT ACCTGACGAA CTCAACTTCC GGCGGAGGAA GCTGTATCCA 
CCAGAGGACA CAACCCGATG TTTTGGCATT CTGACAGCTA AACCAATACC CCGGATCCCT 
CATTTCCCTG TGTACACTCG TTCTGGTGAG GTGACCATCT CCATTGAGCT GCAGAAGTCG 
GGCTTTAGTC TAAGTGCGGA GCAGCTGGAG CTGATCACAC GTCTGCATCA GTACATCTTC 
TCCCACATCC TCCGTCTGGA AAAGCCAGCA CTAGAGTTTA AGCCTGTAGA GGCAGATTCT 
GCCTACTGTG TTCTACCTCT CAACATCGTT GAGGATTCCA ATACTTTGGA TCTGGATTTC 
AAGTTCATGG AGGATATTGA AAAGTCTGAA GCACGCATTG GCATTCCAAA CACTCAGTAC 
ACCAAGCAAA ACCCATTCAT CTTCAAATTG GAGGACTACC AGGATGCTGT CATCATTCCA 
AGGTATCGTA ATTTTGACCA GCCGCATCGG TTCTACGTGG CTGACGTTTA CACAGACCTC 
ACCCCGCTCA GTAAATTCCC CTCTCCAGAG TACGAGACTT TTGCCGAATA CTATAAAACC 
AAGTACAACC TGGACCTGTC AAATGTTAAT CAGCCCCTAC TGGATGTGGA CCACACATCT 
TCAAGATTGA ATCTTTTAAC TCCCCGGCAC CTTAATCAAA AGGGGAAAGC CCTTCCTCTC 
AGCAGTGCGG AGAAGAGAAA GGCCAAGTGG GAAAGCCTAC AAAACAAACA GATTTTAGTT 
CCGGAGCTCT GTGCCATTCA CCCCATCCCA GCCTCTTTGT GGAGGAAAGC CGTCTGCCTT 
CCCAGCATTC TCTATAGACT TCACTGCCTT CTGACGGCCG AAGAGCTGCG GTCTCAGACA 
GCCATCGATG CTGGCGTAGG GGCTCAGACC TTGCCTCCTG ATTTCAGGTA TCCTAATTTG 
GATTTCGGAT GGAAGAAATC CATCGACAGC AAGTCTTTCA TCTCCTGCCC CAGTGCATGT 
ATGGAAGAAG ACGACGATCA CTGTAAACTC GGCACAAGCT CCGATTCAAA CCACACTGCC 
CCCGAGAGCT GCAGTATGGA GGTCTCCCAG CCTCCAGAAG GCGCTCCCAA CACACCAGAT 
GAAAAGCTAG AAACCCTCAC TCTCCCTGTG ACTGATCTAA ACAAAGACTG TTTCCCAAAC 
CTCCCAAACG GCACTCAGGC CGACAGCGAC GACCTCCCTC ACAGAAGTGA CGTTTGCCAA 
TGCTCTCAGC TGGGCCCTCT GGAGAGAGAC CTATCAACAC AAACCACTAC CTCAGTTTCA 
GTGCGGCCCT CTCCAGCTGG AGAACCACAG CCATGGCCCA GCGATGAATG TACAGGCAGG 
AGCTCGGATC TCTGCGACCC ACATGTGAAG AAACCTACCT CAAAGCATTG CCCTAAATCG 
GAGACGGCGA CTAGCACCCC AGCACCTTCA GAAACGTCAT CAGAGGACTG TAGATCAGCC 
TGCGCTGGTC CAGCCTGGGA TTCGCCCAAA ACCCTGGGCC CGAACCCCGG GCTCATCCTG 
CAGGCCCTGA CTCTCTCCAA CGCCAGTGAC GGCTTCAATC TGGAACGCCT GGAGATGCTG 
GGCGACTCCT TCCTGAAACA CGCCATCACC ACATACCTGT TCTGCACTTA CCCTGACGCC 
CACGAGGGCA GACTGTCCTA CATGAGAAGC AAGAAGGTCA GCAACTGTAA CCTGTATCGG 
CTGGGGAAGA AGAAGGGTCT TCCGAGTCGA ATGGTGGTGT CCATCTTTGA CCCGCCGGTA 
AACTGGCTTC CTCCAGGCTA CGTCGTGAAT CAGGACAAAA GCAGCACAGA CAAGTGGGAC 
TCAGATGAGA ATAAAGATTT AGCGAATGGA AAAGCCAGCG ATGACGAAGA CGAGGATGAC 
GATGATGAGC CGGAAGAAGC AGAGGTCGAG CCATCTAAAG AAGATGTAAA TGTTGAAGAC 
GACCTGGAGT ATTATTACGA ACACATCCGC TTCATCGACA GCATGTTAAT CGGCTCTGGC 
GCTTTCGGCA AGAAGATCTC CCTCCAGCCC ACAGACCCCG GCTACGAATG GAAAGCTCCT 
AAAAAAGCCC ACAACTCCCA CTTCTCGCCA GACGGGGGTG CTGACGAGTT CGATTACAGC 
TCCTGGGACG CCATGTGTTA CCTGGACCCC AGTAAAGCAG GTGAGGAGGA CGATTTCGTG 
GTGGGCTTCT GGAATCCCTC TGAGGAAAAC TGCGGCACGG ACATCGGGAA ACAGTCCATC 
TCTTATGACC TTCACACGGA GCAGTGCATC GCAGATAAGA GCATAGCGGA CTGTGTGGAG 
GCTCTGCTGG GCTGCTATCT GACCAGCTGT GGTGAGCGAG CGGCTCAGCT GTTTCTCTGC 
TCTCTGGGCC TGAAGGTGCT ACCACCGGAG AAGCAGAGTT CGGGAGGCTC TGCCGAGCTC 
CAGTACGGCT GGCTGAAGAT CCCACCACGC TGCATGTTCG AACATCCTGA CGCTGAACGC 
ACGCTCAACC ACCTCATCTC TGGCTTCCTG AACTTCGAGA GCAAGATCAA CTACACGTTT 
AAGAACAAGG CGTACCTGCT GCAGGCCTTC ACACACGCCT CGTATCATTA CAACACCATT 
ACAGATTGTT ATCAGCGGTT GGAGTTTCTT GGTGATGCAA TTCTAGACTA CCTCATAACC 
AAGCACCTTT ATGAAGACCC CCGGCAGCAC TCGCCCGGCG TGCTGACCGA CCTGCGCTCT 
GCACTTGTCA ACAACACCAT ATTCGCTTCC CTGGCTGTCA AGTATGATTA CCACAAGTAC 
TTCAAAGCTG TCTCGCCAGA ACTCTTCCAC GTGATCGACG ATTTTGTGCA ATTTCAACTG 
GAGAAGAATG AAATGCAAGG AATGGATTCA GAGCTTCGCC GATCGGAGGA AGATGAAGAG 
AAAGAGGAAG ACATTGAAGT CCCCAAAGCA ATGGGAGACA TCTTTGAATC GCTAGCTGGT 
GCAATTTACA TGGATAGCGG GATGTCACTT GAGACAGTTT GGCAAGTGTA TTATCCAATG 
ATGAGGCCGC TCATAGAAAA ATTCTCCGCT AATGTCCCTC GATCCCCAGT GCGTGAACTC 
CTGGAGATGG AGCCAGAGAC AGCTAAGTTC AGCCCTGCGG AAAGAACTTA TGATGGAAAG 
GTACGGGTGA CGGTGGAAGT CGTGGGCAAA GGCAAGTTCA AAGGCGTAGG ACGCAGCTAC 
CGGATAGCCA AGTCAGCAGC CGCCCGCCGA GCCCTGCGCA GTCTCAAAGC CAACCAACCT 
CAGGTCCAAA ACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dicer1 ( XM_005158665.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005158722.1 Danio rerio dicer 1, ribonuclease type III (dicer1), transcript variant X2, mRNA.

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CloneID	ODa03830
Clone ID Related Accession (Same CDS sequence)	XM_005158665.3 , XM_005158666.3 , XM_021467115.1 , XM_005158665.4 , XM_021467114.1 , XM_021467116.1 , NM_001161453.2 , XM_005158666.4
Accession Version	XM_005158665.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5598bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005158665.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGCC TACAGCTGGT GACCCCTGCC TCCTCACCCA TGGGGCCCTT CTTTGGTCTC 
CCGTGGCAAC AGGAAGCTAT CCATGACAAC ATATACACAC CAAGGAAATA TCAGGTTGAA 
CTTCTTGAAG CAGCTCTTGA ACATAATACT ATAGTCTGCT TAAATACTGG CTCAGGGAAG 
ACCTTTATCG CAGTGCTCCT AATCAAAGAA CTCTCCCACC AAATTCGTGG CGAGAATGGG 
AAGAGGACAG TTTTCCTGGT GAATGCAGCG TCATCTGTGG CTCAGCAAGC ATCTACTGTA 
AGAACACACT CTGATCTCCA AGTAGGCGAC TATATGTCAG AGGACATGAC ATCATGGCCC 
GAGGAGATGT GGAACAGAGA GATGATAGAG AATCAGGTGC TGGTGATGAC GTGCCACATC 
TTCCTGCATG TTTTAAAGAA TGGAGTCTTG CCACTATCAA AGATCAACCT GCTGGTTTTT 
GACGAATGCC ACCTTGCAAT CACTGGCCAT CCTTATCGGG AAATAATGAA GATTTGTGAA 
GGTTGCCCCA GCTGTCCTCG CATTTTGGGT CTCACTGCTT CCATTTTAAA TGGCAAATGT 
GACCCTTGTG ATTTGGAGGA GAAAATTCAG AACCTGGAGA AGATTCTTCA GAGCAATGCA 
GAAACTGCCA CTGATCTTGT GGTGCTGGAT AGGTATGCGT CGCAGCCGCG TGAGGAGGTG 
CTGGATTGTG GACAGTACCA AGATCAGAGT GGGCTCTCGG AGAGGCTTTT AAATGAGCTG 
GACGAAGCCC TCAATTTTCT AAACGATTGC AACTTGTCTG TACATAGAGA AGATAGAGAC 
CCCACGTTTA TCTCCAAACA GGTGCTGAAT GACTGTCGTG CTGTGTTGAC TGTGCTGGGC 
CCTTGGTGTG CGGATAAAGC TGCAGGAATC ATGGTTCGGG AGCTGCAGAA ATATATTAAA 
CATGAACAGG AAGAGCTAAA CCGCAAGTTC CTGTTGTTCA CCGACACCAT CCTGAGGAAA 
ATCCATGCCT TATGTGAGGA GCATTTTTCC CCAGCTTCCC TCGACCTGAA GTTTGTCACT 
CCCAAGGTCA TCAGACTTTT GGAGATTCTC CACGAGTACA AGCCTTTCGA ACGGCAGCAG 
TTTGAGAGTG TGGAGTGGTA CAACAATCGC AATCAGGACA ATTATGTTTC TTGGAGTGAT 
TCTGAAGATG ATGATGAGGA CGAAGAGGCG GAGGCAAAGG AGAAAACCGA AGCAAATTTT 
CCCTCGCCGT TCACAAATAT CCTCTGTGGA ATCATCTTTG TGGAAAGGCG ATATACAGCG 
GTTGTTTTAA ACAGACTTAT TAAGGAGGCA GGAAAGCAAG ACCCAGAGCT GGCTTACATC 
AGTAGCAACT TCATCACAGG GCACAGCATC GGCAAGAACC AGCCTCGCAA CAAGCAGATG 
GAGGTGGAGT TCAGAAAACA AGAGGAAGTC TTGAGGAAAT TCAGAGCCCA TGAAACCAAC 
TTGCTCATTG CAACCAGCAT TGTGGAGGAA GGCGTCGATA TCCCAAAGTG CAATTTGGTC 
GTTCGGTTTG ACCTGCCGAC AGAGTACCGG TCGTACGTGC AATCCAAAGG TCGAGCCCGT 
GCTCCAGTCT CCAACTACAT CATGCTAGCA GACAGCGAAC GGACTAAAAC CTTCCAAGAA 
GACCTCAAGA CCTACAAGGC CATAGAGAAG ATCCTCCGAA ATAAGTGCTC GAAGTCGGCT 
GAATGCAATG ATTTTGAGCT GGAGCCTGTG ACCGATGACG ACAATGTTCT CCCACCGTAC 
GTCCTGCGCT CAGAGGACGG CGGGCCTCGT GTCACCATGA ACACTGCCAT TGGGCACGTC 
AACCGATATT GTGCGCGCTT GCCCAGTGAT CCGTTCACTC ATCTCGCTCC GAAGTGTAAA 
ACTGTAGAGA TGAACACCGG AGGGTATCGA TCCACACTCT TCCTACCTAT CAACTCACCA 
CTCAGAGTTC CTGTCACCGG GCCAGTAATG AATTGTGCAA GACTGGCTGA GAAAGCTGTC 
GCTCTTCTCT GTTGTGAAAA ACTCCACAAA ATTGGTGAAC TGGATGATCA TCTGATGCCT 
GTGGGAAAGG AAACTGTGAA GTATGAGGAG GAGCTGGACC TGCATGATGA AGAGGAGACA 
AGCGTGCCAG GAAGACCAGG CTCCACTAAA CGGAGACAGT GCTCCCCAAA AGCCATACCA 
GAGTGTCTGC GGGGTTGTTA CCCTGTGCCA GAGCAGCCTT GCTACCTTTA CGTTATTGGG 
ATGGTCCTCA CCACCCCTCT ACCTGACGAA CTCAACTTCC GGCGGAGGAA GCTGTATCCA 
CCAGAGGACA CAACCCGATG TTTTGGCATT CTGACAGCTA AACCAATACC CCGGATCCCT 
CATTTCCCTG TGTACACTCG TTCTGGTGAG GTGACCATCT CCATTGAGCT GCAGAAGTCG 
GGCTTTAGTC TAAGTGCGGA GCAGCTGGAG CTGATCACAC GTCTGCATCA GTACATCTTC 
TCCCACATCC TCCGTCTGGA AAAGCCAGCA CTAGAGTTTA AGCCTGTAGA GGCAGATTCT 
GCCTACTGTG TTCTACCTCT CAACATCGTT GAGGATTCCA ATACTTTGGA TCTGGATTTC 
AAGTTCATGG AGGATATTGA AAAGTCTGAA GCACGCATTG GCATTCCAAA CACTCAGTAC 
ACCAAGCAAA ACCCATTCAT CTTCAAATTG GAGGACTACC AGGATGCTGT CATCATTCCA 
AGGTATCGTA ATTTTGACCA GCCGCATCGG TTCTACGTGG CTGACGTTTA CACAGACCTC 
ACCCCGCTCA GTAAATTCCC CTCTCCAGAG TACGAGACTT TTGCCGAATA CTATAAAACC 
AAGTACAACC TGGACCTGTC AAATGTTAAT CAGCCCCTAC TGGATGTGGA CCACACATCT 
TCAAGATTGA ATCTTTTAAC TCCCCGGCAC CTTAATCAAA AGGGGAAAGC CCTTCCTCTC 
AGCAGTGCGG AGAAGAGAAA GGCCAAGTGG GAAAGCCTAC AAAACAAACA GATTTTAGTT 
CCGGAGCTCT GTGCCATTCA CCCCATCCCA GCCTCTTTGT GGAGGAAAGC CGTCTGCCTT 
CCCAGCATTC TCTATAGACT TCACTGCCTT CTGACGGCCG AAGAGCTGCG GTCTCAGACA 
GCCATCGATG CTGGCGTAGG GGCTCAGACC TTGCCTCCTG ATTTCAGGTA TCCTAATTTG 
GATTTCGGAT GGAAGAAATC CATCGACAGC AAGTCTTTCA TCTCCTGCCC CAGTGCATGT 
ATGGAAGAAG ACGACGATCA CTGTAAACTC GGCACAAGCT CCGATTCAAA CCACACTGCC 
CCCGAGAGCT GCAGTATGGA GGTCTCCCAG CCTCCAGAAG GCGCTCCCAA CACACCAGAT 
GAAAAGCTAG AAACCCTCAC TCTCCCTGTG ACTGATCTAA ACAAAGACTG TTTCCCAAAC 
CTCCCAAACG GCACTCAGGC CGACAGCGAC GACCTCCCTC ACAGAAGTGA CGTTTGCCAA 
TGCTCTCAGC TGGGCCCTCT GGAGAGAGAC CTATCAACAC AAACCACTAC CTCAGTTTCA 
GTGCGGCCCT CTCCAGCTGG AGAACCACAG CCATGGCCCA GCGATGAATG TACAGGCAGG 
AGCTCGGATC TCTGCGACCC ACATGTGAAG AAACCTACCT CAAAGCATTG CCCTAAATCG 
GAGACGGCGA CTAGCACCCC AGCACCTTCA GAAACGTCAT CAGAGGACTG TAGATCAGCC 
TGCGCTGGTC CAGCCTGGGA TTCGCCCAAA ACCCTGGGCC CGAACCCCGG GCTCATCCTG 
CAGGCCCTGA CTCTCTCCAA CGCCAGTGAC GGCTTCAATC TGGAACGCCT GGAGATGCTG 
GGCGACTCCT TCCTGAAACA CGCCATCACC ACATACCTGT TCTGCACTTA CCCTGACGCC 
CACGAGGGCA GACTGTCCTA CATGAGAAGC AAGAAGGTCA GCAACTGTAA CCTGTATCGG 
CTGGGGAAGA AGAAGGGTCT TCCGAGTCGA ATGGTGGTGT CCATCTTTGA CCCGCCGGTA 
AACTGGCTTC CTCCAGGCTA CGTCGTGAAT CAGGACAAAA GCAGCACAGA CAAGTGGGAC 
TCAGATGAGA ATAAAGATTT AGCGAATGGA AAAGCCAGCG ATGACGAAGA CGAGGATGAC 
GATGATGAGC CGGAAGAAGC AGAGGTCGAG CCATCTAAAG AAGATGTAAA TGTTGAAGAC 
GACCTGGAGT ATTATTACGA ACACATCCGC TTCATCGACA GCATGTTAAT CGGCTCTGGC 
GCTTTCGGCA AGAAGATCTC CCTCCAGCCC ACAGACCCCG GCTACGAATG GAAAGCTCCT 
AAAAAAGCCC ACAACTCCCA CTTCTCGCCA GACGGGGGTG CTGACGAGTT CGATTACAGC 
TCCTGGGACG CCATGTGTTA CCTGGACCCC AGTAAAGCAG GTGAGGAGGA CGATTTCGTG 
GTGGGCTTCT GGAATCCCTC TGAGGAAAAC TGCGGCACGG ACATCGGGAA ACAGTCCATC 
TCTTATGACC TTCACACGGA GCAGTGCATC GCAGATAAGA GCATAGCGGA CTGTGTGGAG 
GCTCTGCTGG GCTGCTATCT GACCAGCTGT GGTGAGCGAG CGGCTCAGCT GTTTCTCTGC 
TCTCTGGGCC TGAAGGTGCT ACCACCGGAG AAGCAGAGTT CGGGAGGCTC TGCCGAGCTC 
CAGTACGGCT GGCTGAAGAT CCCACCACGC TGCATGTTCG AACATCCTGA CGCTGAACGC 
ACGCTCAACC ACCTCATCTC TGGCTTCCTG AACTTCGAGA GCAAGATCAA CTACACGTTT 
AAGAACAAGG CGTACCTGCT GCAGGCCTTC ACACACGCCT CGTATCATTA CAACACCATT 
ACAGATTGTT ATCAGCGGTT GGAGTTTCTT GGTGATGCAA TTCTAGACTA CCTCATAACC 
AAGCACCTTT ATGAAGACCC CCGGCAGCAC TCGCCCGGCG TGCTGACCGA CCTGCGCTCT 
GCACTTGTCA ACAACACCAT ATTCGCTTCC CTGGCTGTCA AGTATGATTA CCACAAGTAC 
TTCAAAGCTG TCTCGCCAGA ACTCTTCCAC GTGATCGACG ATTTTGTGCA ATTTCAACTG 
GAGAAGAATG AAATGCAAGG AATGGATTCA GAGCTTCGCC GATCGGAGGA AGATGAAGAG 
AAAGAGGAAG ACATTGAAGT CCCCAAAGCA ATGGGAGACA TCTTTGAATC GCTAGCTGGT 
GCAATTTACA TGGATAGCGG GATGTCACTT GAGACAGTTT GGCAAGTGTA TTATCCAATG 
ATGAGGCCGC TCATAGAAAA ATTCTCCGCT AATGTCCCTC GATCCCCAGT GCGTGAACTC 
CTGGAGATGG AGCCAGAGAC AGCTAAGTTC AGCCCTGCGG AAAGAACTTA TGATGGAAAG 
GTACGGGTGA CGGTGGAAGT CGTGGGCAAA GGCAAGTTCA AAGGCGTAGG ACGCAGCTAC 
CGGATAGCCA AGTCAGCAGC CGCCCGCCGA GCCCTGCGCA GTCTCAAAGC CAACCAACCT 
CAGGTCCAAA ACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158722.1
CDS	379..5976
Translation	MAGLQLVTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALEHNTIVCLNTGSGKTFIAVLLIKELSHQIRGENGKRTVFLVNAASSVAQQASTVRTHSDLQVGDYMSEDMTSWPEEMWNREMIENQVLVMTCHIFLHVLKNGVLPLSKINLLVFDECHLAITGHPYREIMKICEGCPSCPRILGLTASILNGKCDPCDLEEKIQNLEKILQSNAETATDLVVLDRYASQPREEVLDCGQYQDQSGLSERLLNELDEALNFLNDCNLSVHREDRDPTFISKQVLNDCRAVLTVLGPWCADKAAGIMVRELQKYIKHEQEELNRKFLLFTDTILRKIHALCEEHFSPASLDLKFVTPKVIRLLEILHEYKPFERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEAEAKEKTEANFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHSIGKNQPRNKQMEVEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPVSNYIMLADSERTKTFQEDLKTYKAIEKILRNKCSKSAECNDFELEPVTDDDNVLPPYVLRSEDGGPRVTMNTAIGHVNRYCARLPSDPFTHLAPKCKTVEMNTGGYRSTLFLPINSPLRVPVTGPVMNCARLAEKAVALLCCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCSPKAIPECLRGCYPVPEQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPRIPHFPVYTRSGEVTISIELQKSGFSLSAEQLELITRLHQYIFSHILRLEKPALEFKPVEADSAYCVLPLNIVEDSNTLDLDFKFMEDIEKSEARIGIPNTQYTKQNPFIFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLSNVNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRSQTAIDAGVGAQTLPPDFRYPNLDFGWKKSIDSKSFISCPSACMEEDDDHCKLGTSSDSNHTAPESCSMEVSQPPEGAPNTPDEKLETLTLPVTDLNKDCFPNLPNGTQADSDDLPHRSDVCQCSQLGPLERDLSTQTTTSVSVRPSPAGEPQPWPSDECTGRSSDLCDPHVKKPTSKHCPKSETATSTPAPSETSSEDCRSACAGPAWDSPKTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSSTDKWDSDENKDLANGKASDDEDEDDDDEPEEAEVEPSKEDVNVEDDLEYYYEHIRFIDSMLIGSGAFGKKISLQPTDPGYEWKAPKKAHNSHFSPDGGADEFDYSSWDAMCYLDPSKAGEEDDFVVGFWNPSEENCGTDIGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPPEKQSSGGSAELQYGWLKIPPRCMFEHPDAERTLNHLISGFLNFESKINYTFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVQNN

Target ORF information:

RefSeq Version	XM_005158665.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio dicer 1, ribonuclease type III (dicer1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158665.4

ORF Insert Sequence:

ATGGCTGGCC TACAGCTGGT GACCCCTGCC TCCTCACCCA TGGGGCCCTT CTTTGGTCTC 
CCGTGGCAAC AGGAAGCTAT CCATGACAAC ATATACACAC CAAGGAAATA TCAGGTTGAA 
CTTCTTGAAG CAGCTCTTGA ACATAATACT ATAGTCTGCT TAAATACTGG CTCAGGGAAG 
ACCTTTATCG CAGTGCTCCT AATCAAAGAA CTCTCCCACC AAATTCGTGG CGAGAATGGG 
AAGAGGACAG TTTTCCTGGT GAATGCAGCG TCATCTGTGG CTCAGCAAGC ATCTACTGTA 
AGAACACACT CTGATCTCCA AGTAGGCGAC TATATGTCAG AGGACATGAC ATCATGGCCC 
GAGGAGATGT GGAACAGAGA GATGATAGAG AATCAGGTGC TGGTGATGAC GTGCCACATC 
TTCCTGCATG TTTTAAAGAA TGGAGTCTTG CCACTATCAA AGATCAACCT GCTGGTTTTT 
GACGAATGCC ACCTTGCAAT CACTGGCCAT CCTTATCGGG AAATAATGAA GATTTGTGAA 
GGTTGCCCCA GCTGTCCTCG CATTTTGGGT CTCACTGCTT CCATTTTAAA TGGCAAATGT 
GACCCTTGTG ATTTGGAGGA GAAAATTCAG AACCTGGAGA AGATTCTTCA GAGCAATGCA 
GAAACTGCCA CTGATCTTGT GGTGCTGGAT AGGTATGCGT CGCAGCCGCG TGAGGAGGTG 
CTGGATTGTG GACAGTACCA AGATCAGAGT GGGCTCTCGG AGAGGCTTTT AAATGAGCTG 
GACGAAGCCC TCAATTTTCT AAACGATTGC AACTTGTCTG TACATAGAGA AGATAGAGAC 
CCCACGTTTA TCTCCAAACA GGTGCTGAAT GACTGTCGTG CTGTGTTGAC TGTGCTGGGC 
CCTTGGTGTG CGGATAAAGC TGCAGGAATC ATGGTTCGGG AGCTGCAGAA ATATATTAAA 
CATGAACAGG AAGAGCTAAA CCGCAAGTTC CTGTTGTTCA CCGACACCAT CCTGAGGAAA 
ATCCATGCCT TATGTGAGGA GCATTTTTCC CCAGCTTCCC TCGACCTGAA GTTTGTCACT 
CCCAAGGTCA TCAGACTTTT GGAGATTCTC CACGAGTACA AGCCTTTCGA ACGGCAGCAG 
TTTGAGAGTG TGGAGTGGTA CAACAATCGC AATCAGGACA ATTATGTTTC TTGGAGTGAT 
TCTGAAGATG ATGATGAGGA CGAAGAGGCG GAGGCAAAGG AGAAAACCGA AGCAAATTTT 
CCCTCGCCGT TCACAAATAT CCTCTGTGGA ATCATCTTTG TGGAAAGGCG ATATACAGCG 
GTTGTTTTAA ACAGACTTAT TAAGGAGGCA GGAAAGCAAG ACCCAGAGCT GGCTTACATC 
AGTAGCAACT TCATCACAGG GCACAGCATC GGCAAGAACC AGCCTCGCAA CAAGCAGATG 
GAGGTGGAGT TCAGAAAACA AGAGGAAGTC TTGAGGAAAT TCAGAGCCCA TGAAACCAAC 
TTGCTCATTG CAACCAGCAT TGTGGAGGAA GGCGTCGATA TCCCAAAGTG CAATTTGGTC 
GTTCGGTTTG ACCTGCCGAC AGAGTACCGG TCGTACGTGC AATCCAAAGG TCGAGCCCGT 
GCTCCAGTCT CCAACTACAT CATGCTAGCA GACAGCGAAC GGACTAAAAC CTTCCAAGAA 
GACCTCAAGA CCTACAAGGC CATAGAGAAG ATCCTCCGAA ATAAGTGCTC GAAGTCGGCT 
GAATGCAATG ATTTTGAGCT GGAGCCTGTG ACCGATGACG ACAATGTTCT CCCACCGTAC 
GTCCTGCGCT CAGAGGACGG CGGGCCTCGT GTCACCATGA ACACTGCCAT TGGGCACGTC 
AACCGATATT GTGCGCGCTT GCCCAGTGAT CCGTTCACTC ATCTCGCTCC GAAGTGTAAA 
ACTGTAGAGA TGAACACCGG AGGGTATCGA TCCACACTCT TCCTACCTAT CAACTCACCA 
CTCAGAGTTC CTGTCACCGG GCCAGTAATG AATTGTGCAA GACTGGCTGA GAAAGCTGTC 
GCTCTTCTCT GTTGTGAAAA ACTCCACAAA ATTGGTGAAC TGGATGATCA TCTGATGCCT 
GTGGGAAAGG AAACTGTGAA GTATGAGGAG GAGCTGGACC TGCATGATGA AGAGGAGACA 
AGCGTGCCAG GAAGACCAGG CTCCACTAAA CGGAGACAGT GCTCCCCAAA AGCCATACCA 
GAGTGTCTGC GGGGTTGTTA CCCTGTGCCA GAGCAGCCTT GCTACCTTTA CGTTATTGGG 
ATGGTCCTCA CCACCCCTCT ACCTGACGAA CTCAACTTCC GGCGGAGGAA GCTGTATCCA 
CCAGAGGACA CAACCCGATG TTTTGGCATT CTGACAGCTA AACCAATACC CCGGATCCCT 
CATTTCCCTG TGTACACTCG TTCTGGTGAG GTGACCATCT CCATTGAGCT GCAGAAGTCG 
GGCTTTAGTC TAAGTGCGGA GCAGCTGGAG CTGATCACAC GTCTGCATCA GTACATCTTC 
TCCCACATCC TCCGTCTGGA AAAGCCAGCA CTAGAGTTTA AGCCTGTAGA GGCAGATTCT 
GCCTACTGTG TTCTACCTCT CAACATCGTT GAGGATTCCA ATACTTTGGA TCTGGATTTC 
AAGTTCATGG AGGATATTGA AAAGTCTGAA GCACGCATTG GCATTCCAAA CACTCAGTAC 
ACCAAGCAAA ACCCATTCAT CTTCAAATTG GAGGACTACC AGGATGCTGT CATCATTCCA 
AGGTATCGTA ATTTTGACCA GCCGCATCGG TTCTACGTGG CTGACGTTTA CACAGACCTC 
ACCCCGCTCA GTAAATTCCC CTCTCCAGAG TACGAGACTT TTGCCGAATA CTATAAAACC 
AAGTACAACC TGGACCTGTC AAATGTTAAT CAGCCCCTAC TGGATGTGGA CCACACATCT 
TCAAGATTGA ATCTTTTAAC TCCCCGGCAC CTTAATCAAA AGGGGAAAGC CCTTCCTCTC 
AGCAGTGCGG AGAAGAGAAA GGCCAAGTGG GAAAGCCTAC AAAACAAACA GATTTTAGTT 
CCGGAGCTCT GTGCCATTCA CCCCATCCCA GCCTCTTTGT GGAGGAAAGC CGTCTGCCTT 
CCCAGCATTC TCTATAGACT TCACTGCCTT CTGACGGCCG AAGAGCTGCG GTCTCAGACA 
GCCATCGATG CTGGCGTAGG GGCTCAGACC TTGCCTCCTG ATTTCAGGTA TCCTAATTTG 
GATTTCGGAT GGAAGAAATC CATCGACAGC AAGTCTTTCA TCTCCTGCCC CAGTGCATGT 
ATGGAAGAAG ACGACGATCA CTGTAAACTC GGCACAAGCT CCGATTCAAA CCACACTGCC 
CCCGAGAGCT GCAGTATGGA GGTCTCCCAG CCTCCAGAAG GCGCTCCCAA CACACCAGAT 
GAAAAGCTAG AAACCCTCAC TCTCCCTGTG ACTGATCTAA ACAAAGACTG TTTCCCAAAC 
CTCCCAAACG GCACTCAGGC CGACAGCGAC GACCTCCCTC ACAGAAGTGA CGTTTGCCAA 
TGCTCTCAGC TGGGCCCTCT GGAGAGAGAC CTATCAACAC AAACCACTAC CTCAGTTTCA 
GTGCGGCCCT CTCCAGCTGG AGAACCACAG CCATGGCCCA GCGATGAATG TACAGGCAGG 
AGCTCGGATC TCTGCGACCC ACATGTGAAG AAACCTACCT CAAAGCATTG CCCTAAATCG 
GAGACGGCGA CTAGCACCCC AGCACCTTCA GAAACGTCAT CAGAGGACTG TAGATCAGCC 
TGCGCTGGTC CAGCCTGGGA TTCGCCCAAA ACCCTGGGCC CGAACCCCGG GCTCATCCTG 
CAGGCCCTGA CTCTCTCCAA CGCCAGTGAC GGCTTCAATC TGGAACGCCT GGAGATGCTG 
GGCGACTCCT TCCTGAAACA CGCCATCACC ACATACCTGT TCTGCACTTA CCCTGACGCC 
CACGAGGGCA GACTGTCCTA CATGAGAAGC AAGAAGGTCA GCAACTGTAA CCTGTATCGG 
CTGGGGAAGA AGAAGGGTCT TCCGAGTCGA ATGGTGGTGT CCATCTTTGA CCCGCCGGTA 
AACTGGCTTC CTCCAGGCTA CGTCGTGAAT CAGGACAAAA GCAGCACAGA CAAGTGGGAC 
TCAGATGAGA ATAAAGATTT AGCGAATGGA AAAGCCAGCG ATGACGAAGA CGAGGATGAC 
GATGATGAGC CGGAAGAAGC AGAGGTCGAG CCATCTAAAG AAGATGTAAA TGTTGAAGAC 
GACCTGGAGT ATTATTACGA ACACATCCGC TTCATCGACA GCATGTTAAT CGGCTCTGGC 
GCTTTCGGCA AGAAGATCTC CCTCCAGCCC ACAGACCCCG GCTACGAATG GAAAGCTCCT 
AAAAAAGCCC ACAACTCCCA CTTCTCGCCA GACGGGGGTG CTGACGAGTT CGATTACAGC 
TCCTGGGACG CCATGTGTTA CCTGGACCCC AGTAAAGCAG GTGAGGAGGA CGATTTCGTG 
GTGGGCTTCT GGAATCCCTC TGAGGAAAAC TGCGGCACGG ACATCGGGAA ACAGTCCATC 
TCTTATGACC TTCACACGGA GCAGTGCATC GCAGATAAGA GCATAGCGGA CTGTGTGGAG 
GCTCTGCTGG GCTGCTATCT GACCAGCTGT GGTGAGCGAG CGGCTCAGCT GTTTCTCTGC 
TCTCTGGGCC TGAAGGTGCT ACCACCGGAG AAGCAGAGTT CGGGAGGCTC TGCCGAGCTC 
CAGTACGGCT GGCTGAAGAT CCCACCACGC TGCATGTTCG AACATCCTGA CGCTGAACGC 
ACGCTCAACC ACCTCATCTC TGGCTTCCTG AACTTCGAGA GCAAGATCAA CTACACGTTT 
AAGAACAAGG CGTACCTGCT GCAGGCCTTC ACACACGCCT CGTATCATTA CAACACCATT 
ACAGATTGTT ATCAGCGGTT GGAGTTTCTT GGTGATGCAA TTCTAGACTA CCTCATAACC 
AAGCACCTTT ATGAAGACCC CCGGCAGCAC TCGCCCGGCG TGCTGACCGA CCTGCGCTCT 
GCACTTGTCA ACAACACCAT ATTCGCTTCC CTGGCTGTCA AGTATGATTA CCACAAGTAC 
TTCAAAGCTG TCTCGCCAGA ACTCTTCCAC GTGATCGACG ATTTTGTGCA ATTTCAACTG 
GAGAAGAATG AAATGCAAGG AATGGATTCA GAGCTTCGCC GATCGGAGGA AGATGAAGAG 
AAAGAGGAAG ACATTGAAGT CCCCAAAGCA ATGGGAGACA TCTTTGAATC GCTAGCTGGT 
GCAATTTACA TGGATAGCGG GATGTCACTT GAGACAGTTT GGCAAGTGTA TTATCCAATG 
ATGAGGCCGC TCATAGAAAA ATTCTCCGCT AATGTCCCTC GATCCCCAGT GCGTGAACTC 
CTGGAGATGG AGCCAGAGAC AGCTAAGTTC AGCCCTGCGG AAAGAACTTA TGATGGAAAG 
GTACGGGTGA CGGTGGAAGT CGTGGGCAAA GGCAAGTTCA AAGGCGTAGG ACGCAGCTAC 
CGGATAGCCA AGTCAGCAGC CGCCCGCCGA GCCCTGCGCA GTCTCAAAGC CAACCAACCT 
CAGGTCCAAA ACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dicer1 ( XM_021467114.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021322789.1 Danio rerio dicer 1, ribonuclease type III (dicer1), transcript variant X3, mRNA.

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CloneID	ODa03830
Clone ID Related Accession (Same CDS sequence)	XM_005158665.3 , XM_005158666.3 , XM_021467115.1 , XM_005158665.4 , XM_021467114.1 , XM_021467116.1 , NM_001161453.2 , XM_005158666.4
Accession Version	XM_021467114.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5598bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGCC TACAGCTGGT GACCCCTGCC TCCTCACCCA TGGGGCCCTT CTTTGGTCTC 
CCGTGGCAAC AGGAAGCTAT CCATGACAAC ATATACACAC CAAGGAAATA TCAGGTTGAA 
CTTCTTGAAG CAGCTCTTGA ACATAATACT ATAGTCTGCT TAAATACTGG CTCAGGGAAG 
ACCTTTATCG CAGTGCTCCT AATCAAAGAA CTCTCCCACC AAATTCGTGG CGAGAATGGG 
AAGAGGACAG TTTTCCTGGT GAATGCAGCG TCATCTGTGG CTCAGCAAGC ATCTACTGTA 
AGAACACACT CTGATCTCCA AGTAGGCGAC TATATGTCAG AGGACATGAC ATCATGGCCC 
GAGGAGATGT GGAACAGAGA GATGATAGAG AATCAGGTGC TGGTGATGAC GTGCCACATC 
TTCCTGCATG TTTTAAAGAA TGGAGTCTTG CCACTATCAA AGATCAACCT GCTGGTTTTT 
GACGAATGCC ACCTTGCAAT CACTGGCCAT CCTTATCGGG AAATAATGAA GATTTGTGAA 
GGTTGCCCCA GCTGTCCTCG CATTTTGGGT CTCACTGCTT CCATTTTAAA TGGCAAATGT 
GACCCTTGTG ATTTGGAGGA GAAAATTCAG AACCTGGAGA AGATTCTTCA GAGCAATGCA 
GAAACTGCCA CTGATCTTGT GGTGCTGGAT AGGTATGCGT CGCAGCCGCG TGAGGAGGTG 
CTGGATTGTG GACAGTACCA AGATCAGAGT GGGCTCTCGG AGAGGCTTTT AAATGAGCTG 
GACGAAGCCC TCAATTTTCT AAACGATTGC AACTTGTCTG TACATAGAGA AGATAGAGAC 
CCCACGTTTA TCTCCAAACA GGTGCTGAAT GACTGTCGTG CTGTGTTGAC TGTGCTGGGC 
CCTTGGTGTG CGGATAAAGC TGCAGGAATC ATGGTTCGGG AGCTGCAGAA ATATATTAAA 
CATGAACAGG AAGAGCTAAA CCGCAAGTTC CTGTTGTTCA CCGACACCAT CCTGAGGAAA 
ATCCATGCCT TATGTGAGGA GCATTTTTCC CCAGCTTCCC TCGACCTGAA GTTTGTCACT 
CCCAAGGTCA TCAGACTTTT GGAGATTCTC CACGAGTACA AGCCTTTCGA ACGGCAGCAG 
TTTGAGAGTG TGGAGTGGTA CAACAATCGC AATCAGGACA ATTATGTTTC TTGGAGTGAT 
TCTGAAGATG ATGATGAGGA CGAAGAGGCG GAGGCAAAGG AGAAAACCGA AGCAAATTTT 
CCCTCGCCGT TCACAAATAT CCTCTGTGGA ATCATCTTTG TGGAAAGGCG ATATACAGCG 
GTTGTTTTAA ACAGACTTAT TAAGGAGGCA GGAAAGCAAG ACCCAGAGCT GGCTTACATC 
AGTAGCAACT TCATCACAGG GCACAGCATC GGCAAGAACC AGCCTCGCAA CAAGCAGATG 
GAGGTGGAGT TCAGAAAACA AGAGGAAGTC TTGAGGAAAT TCAGAGCCCA TGAAACCAAC 
TTGCTCATTG CAACCAGCAT TGTGGAGGAA GGCGTCGATA TCCCAAAGTG CAATTTGGTC 
GTTCGGTTTG ACCTGCCGAC AGAGTACCGG TCGTACGTGC AATCCAAAGG TCGAGCCCGT 
GCTCCAGTCT CCAACTACAT CATGCTAGCA GACAGCGAAC GGACTAAAAC CTTCCAAGAA 
GACCTCAAGA CCTACAAGGC CATAGAGAAG ATCCTCCGAA ATAAGTGCTC GAAGTCGGCT 
GAATGCAATG ATTTTGAGCT GGAGCCTGTG ACCGATGACG ACAATGTTCT CCCACCGTAC 
GTCCTGCGCT CAGAGGACGG CGGGCCTCGT GTCACCATGA ACACTGCCAT TGGGCACGTC 
AACCGATATT GTGCGCGCTT GCCCAGTGAT CCGTTCACTC ATCTCGCTCC GAAGTGTAAA 
ACTGTAGAGA TGAACACCGG AGGGTATCGA TCCACACTCT TCCTACCTAT CAACTCACCA 
CTCAGAGTTC CTGTCACCGG GCCAGTAATG AATTGTGCAA GACTGGCTGA GAAAGCTGTC 
GCTCTTCTCT GTTGTGAAAA ACTCCACAAA ATTGGTGAAC TGGATGATCA TCTGATGCCT 
GTGGGAAAGG AAACTGTGAA GTATGAGGAG GAGCTGGACC TGCATGATGA AGAGGAGACA 
AGCGTGCCAG GAAGACCAGG CTCCACTAAA CGGAGACAGT GCTCCCCAAA AGCCATACCA 
GAGTGTCTGC GGGGTTGTTA CCCTGTGCCA GAGCAGCCTT GCTACCTTTA CGTTATTGGG 
ATGGTCCTCA CCACCCCTCT ACCTGACGAA CTCAACTTCC GGCGGAGGAA GCTGTATCCA 
CCAGAGGACA CAACCCGATG TTTTGGCATT CTGACAGCTA AACCAATACC CCGGATCCCT 
CATTTCCCTG TGTACACTCG TTCTGGTGAG GTGACCATCT CCATTGAGCT GCAGAAGTCG 
GGCTTTAGTC TAAGTGCGGA GCAGCTGGAG CTGATCACAC GTCTGCATCA GTACATCTTC 
TCCCACATCC TCCGTCTGGA AAAGCCAGCA CTAGAGTTTA AGCCTGTAGA GGCAGATTCT 
GCCTACTGTG TTCTACCTCT CAACATCGTT GAGGATTCCA ATACTTTGGA TCTGGATTTC 
AAGTTCATGG AGGATATTGA AAAGTCTGAA GCACGCATTG GCATTCCAAA CACTCAGTAC 
ACCAAGCAAA ACCCATTCAT CTTCAAATTG GAGGACTACC AGGATGCTGT CATCATTCCA 
AGGTATCGTA ATTTTGACCA GCCGCATCGG TTCTACGTGG CTGACGTTTA CACAGACCTC 
ACCCCGCTCA GTAAATTCCC CTCTCCAGAG TACGAGACTT TTGCCGAATA CTATAAAACC 
AAGTACAACC TGGACCTGTC AAATGTTAAT CAGCCCCTAC TGGATGTGGA CCACACATCT 
TCAAGATTGA ATCTTTTAAC TCCCCGGCAC CTTAATCAAA AGGGGAAAGC CCTTCCTCTC 
AGCAGTGCGG AGAAGAGAAA GGCCAAGTGG GAAAGCCTAC AAAACAAACA GATTTTAGTT 
CCGGAGCTCT GTGCCATTCA CCCCATCCCA GCCTCTTTGT GGAGGAAAGC CGTCTGCCTT 
CCCAGCATTC TCTATAGACT TCACTGCCTT CTGACGGCCG AAGAGCTGCG GTCTCAGACA 
GCCATCGATG CTGGCGTAGG GGCTCAGACC TTGCCTCCTG ATTTCAGGTA TCCTAATTTG 
GATTTCGGAT GGAAGAAATC CATCGACAGC AAGTCTTTCA TCTCCTGCCC CAGTGCATGT 
ATGGAAGAAG ACGACGATCA CTGTAAACTC GGCACAAGCT CCGATTCAAA CCACACTGCC 
CCCGAGAGCT GCAGTATGGA GGTCTCCCAG CCTCCAGAAG GCGCTCCCAA CACACCAGAT 
GAAAAGCTAG AAACCCTCAC TCTCCCTGTG ACTGATCTAA ACAAAGACTG TTTCCCAAAC 
CTCCCAAACG GCACTCAGGC CGACAGCGAC GACCTCCCTC ACAGAAGTGA CGTTTGCCAA 
TGCTCTCAGC TGGGCCCTCT GGAGAGAGAC CTATCAACAC AAACCACTAC CTCAGTTTCA 
GTGCGGCCCT CTCCAGCTGG AGAACCACAG CCATGGCCCA GCGATGAATG TACAGGCAGG 
AGCTCGGATC TCTGCGACCC ACATGTGAAG AAACCTACCT CAAAGCATTG CCCTAAATCG 
GAGACGGCGA CTAGCACCCC AGCACCTTCA GAAACGTCAT CAGAGGACTG TAGATCAGCC 
TGCGCTGGTC CAGCCTGGGA TTCGCCCAAA ACCCTGGGCC CGAACCCCGG GCTCATCCTG 
CAGGCCCTGA CTCTCTCCAA CGCCAGTGAC GGCTTCAATC TGGAACGCCT GGAGATGCTG 
GGCGACTCCT TCCTGAAACA CGCCATCACC ACATACCTGT TCTGCACTTA CCCTGACGCC 
CACGAGGGCA GACTGTCCTA CATGAGAAGC AAGAAGGTCA GCAACTGTAA CCTGTATCGG 
CTGGGGAAGA AGAAGGGTCT TCCGAGTCGA ATGGTGGTGT CCATCTTTGA CCCGCCGGTA 
AACTGGCTTC CTCCAGGCTA CGTCGTGAAT CAGGACAAAA GCAGCACAGA CAAGTGGGAC 
TCAGATGAGA ATAAAGATTT AGCGAATGGA AAAGCCAGCG ATGACGAAGA CGAGGATGAC 
GATGATGAGC CGGAAGAAGC AGAGGTCGAG CCATCTAAAG AAGATGTAAA TGTTGAAGAC 
GACCTGGAGT ATTATTACGA ACACATCCGC TTCATCGACA GCATGTTAAT CGGCTCTGGC 
GCTTTCGGCA AGAAGATCTC CCTCCAGCCC ACAGACCCCG GCTACGAATG GAAAGCTCCT 
AAAAAAGCCC ACAACTCCCA CTTCTCGCCA GACGGGGGTG CTGACGAGTT CGATTACAGC 
TCCTGGGACG CCATGTGTTA CCTGGACCCC AGTAAAGCAG GTGAGGAGGA CGATTTCGTG 
GTGGGCTTCT GGAATCCCTC TGAGGAAAAC TGCGGCACGG ACATCGGGAA ACAGTCCATC 
TCTTATGACC TTCACACGGA GCAGTGCATC GCAGATAAGA GCATAGCGGA CTGTGTGGAG 
GCTCTGCTGG GCTGCTATCT GACCAGCTGT GGTGAGCGAG CGGCTCAGCT GTTTCTCTGC 
TCTCTGGGCC TGAAGGTGCT ACCACCGGAG AAGCAGAGTT CGGGAGGCTC TGCCGAGCTC 
CAGTACGGCT GGCTGAAGAT CCCACCACGC TGCATGTTCG AACATCCTGA CGCTGAACGC 
ACGCTCAACC ACCTCATCTC TGGCTTCCTG AACTTCGAGA GCAAGATCAA CTACACGTTT 
AAGAACAAGG CGTACCTGCT GCAGGCCTTC ACACACGCCT CGTATCATTA CAACACCATT 
ACAGATTGTT ATCAGCGGTT GGAGTTTCTT GGTGATGCAA TTCTAGACTA CCTCATAACC 
AAGCACCTTT ATGAAGACCC CCGGCAGCAC TCGCCCGGCG TGCTGACCGA CCTGCGCTCT 
GCACTTGTCA ACAACACCAT ATTCGCTTCC CTGGCTGTCA AGTATGATTA CCACAAGTAC 
TTCAAAGCTG TCTCGCCAGA ACTCTTCCAC GTGATCGACG ATTTTGTGCA ATTTCAACTG 
GAGAAGAATG AAATGCAAGG AATGGATTCA GAGCTTCGCC GATCGGAGGA AGATGAAGAG 
AAAGAGGAAG ACATTGAAGT CCCCAAAGCA ATGGGAGACA TCTTTGAATC GCTAGCTGGT 
GCAATTTACA TGGATAGCGG GATGTCACTT GAGACAGTTT GGCAAGTGTA TTATCCAATG 
ATGAGGCCGC TCATAGAAAA ATTCTCCGCT AATGTCCCTC GATCCCCAGT GCGTGAACTC 
CTGGAGATGG AGCCAGAGAC AGCTAAGTTC AGCCCTGCGG AAAGAACTTA TGATGGAAAG 
GTACGGGTGA CGGTGGAAGT CGTGGGCAAA GGCAAGTTCA AAGGCGTAGG ACGCAGCTAC 
CGGATAGCCA AGTCAGCAGC CGCCCGCCGA GCCCTGCGCA GTCTCAAAGC CAACCAACCT 
CAGGTCCAAA ACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021322789.1
CDS	372..5969
Translation	MAGLQLVTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALEHNTIVCLNTGSGKTFIAVLLIKELSHQIRGENGKRTVFLVNAASSVAQQASTVRTHSDLQVGDYMSEDMTSWPEEMWNREMIENQVLVMTCHIFLHVLKNGVLPLSKINLLVFDECHLAITGHPYREIMKICEGCPSCPRILGLTASILNGKCDPCDLEEKIQNLEKILQSNAETATDLVVLDRYASQPREEVLDCGQYQDQSGLSERLLNELDEALNFLNDCNLSVHREDRDPTFISKQVLNDCRAVLTVLGPWCADKAAGIMVRELQKYIKHEQEELNRKFLLFTDTILRKIHALCEEHFSPASLDLKFVTPKVIRLLEILHEYKPFERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEAEAKEKTEANFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHSIGKNQPRNKQMEVEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPVSNYIMLADSERTKTFQEDLKTYKAIEKILRNKCSKSAECNDFELEPVTDDDNVLPPYVLRSEDGGPRVTMNTAIGHVNRYCARLPSDPFTHLAPKCKTVEMNTGGYRSTLFLPINSPLRVPVTGPVMNCARLAEKAVALLCCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCSPKAIPECLRGCYPVPEQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPRIPHFPVYTRSGEVTISIELQKSGFSLSAEQLELITRLHQYIFSHILRLEKPALEFKPVEADSAYCVLPLNIVEDSNTLDLDFKFMEDIEKSEARIGIPNTQYTKQNPFIFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLSNVNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRSQTAIDAGVGAQTLPPDFRYPNLDFGWKKSIDSKSFISCPSACMEEDDDHCKLGTSSDSNHTAPESCSMEVSQPPEGAPNTPDEKLETLTLPVTDLNKDCFPNLPNGTQADSDDLPHRSDVCQCSQLGPLERDLSTQTTTSVSVRPSPAGEPQPWPSDECTGRSSDLCDPHVKKPTSKHCPKSETATSTPAPSETSSEDCRSACAGPAWDSPKTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSSTDKWDSDENKDLANGKASDDEDEDDDDEPEEAEVEPSKEDVNVEDDLEYYYEHIRFIDSMLIGSGAFGKKISLQPTDPGYEWKAPKKAHNSHFSPDGGADEFDYSSWDAMCYLDPSKAGEEDDFVVGFWNPSEENCGTDIGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPPEKQSSGGSAELQYGWLKIPPRCMFEHPDAERTLNHLISGFLNFESKINYTFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVQNN

Target ORF information:

RefSeq Version	XM_021467114.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio dicer 1, ribonuclease type III (dicer1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021467114.1

ORF Insert Sequence:

ATGGCTGGCC TACAGCTGGT GACCCCTGCC TCCTCACCCA TGGGGCCCTT CTTTGGTCTC 
CCGTGGCAAC AGGAAGCTAT CCATGACAAC ATATACACAC CAAGGAAATA TCAGGTTGAA 
CTTCTTGAAG CAGCTCTTGA ACATAATACT ATAGTCTGCT TAAATACTGG CTCAGGGAAG 
ACCTTTATCG CAGTGCTCCT AATCAAAGAA CTCTCCCACC AAATTCGTGG CGAGAATGGG 
AAGAGGACAG TTTTCCTGGT GAATGCAGCG TCATCTGTGG CTCAGCAAGC ATCTACTGTA 
AGAACACACT CTGATCTCCA AGTAGGCGAC TATATGTCAG AGGACATGAC ATCATGGCCC 
GAGGAGATGT GGAACAGAGA GATGATAGAG AATCAGGTGC TGGTGATGAC GTGCCACATC 
TTCCTGCATG TTTTAAAGAA TGGAGTCTTG CCACTATCAA AGATCAACCT GCTGGTTTTT 
GACGAATGCC ACCTTGCAAT CACTGGCCAT CCTTATCGGG AAATAATGAA GATTTGTGAA 
GGTTGCCCCA GCTGTCCTCG CATTTTGGGT CTCACTGCTT CCATTTTAAA TGGCAAATGT 
GACCCTTGTG ATTTGGAGGA GAAAATTCAG AACCTGGAGA AGATTCTTCA GAGCAATGCA 
GAAACTGCCA CTGATCTTGT GGTGCTGGAT AGGTATGCGT CGCAGCCGCG TGAGGAGGTG 
CTGGATTGTG GACAGTACCA AGATCAGAGT GGGCTCTCGG AGAGGCTTTT AAATGAGCTG 
GACGAAGCCC TCAATTTTCT AAACGATTGC AACTTGTCTG TACATAGAGA AGATAGAGAC 
CCCACGTTTA TCTCCAAACA GGTGCTGAAT GACTGTCGTG CTGTGTTGAC TGTGCTGGGC 
CCTTGGTGTG CGGATAAAGC TGCAGGAATC ATGGTTCGGG AGCTGCAGAA ATATATTAAA 
CATGAACAGG AAGAGCTAAA CCGCAAGTTC CTGTTGTTCA CCGACACCAT CCTGAGGAAA 
ATCCATGCCT TATGTGAGGA GCATTTTTCC CCAGCTTCCC TCGACCTGAA GTTTGTCACT 
CCCAAGGTCA TCAGACTTTT GGAGATTCTC CACGAGTACA AGCCTTTCGA ACGGCAGCAG 
TTTGAGAGTG TGGAGTGGTA CAACAATCGC AATCAGGACA ATTATGTTTC TTGGAGTGAT 
TCTGAAGATG ATGATGAGGA CGAAGAGGCG GAGGCAAAGG AGAAAACCGA AGCAAATTTT 
CCCTCGCCGT TCACAAATAT CCTCTGTGGA ATCATCTTTG TGGAAAGGCG ATATACAGCG 
GTTGTTTTAA ACAGACTTAT TAAGGAGGCA GGAAAGCAAG ACCCAGAGCT GGCTTACATC 
AGTAGCAACT TCATCACAGG GCACAGCATC GGCAAGAACC AGCCTCGCAA CAAGCAGATG 
GAGGTGGAGT TCAGAAAACA AGAGGAAGTC TTGAGGAAAT TCAGAGCCCA TGAAACCAAC 
TTGCTCATTG CAACCAGCAT TGTGGAGGAA GGCGTCGATA TCCCAAAGTG CAATTTGGTC 
GTTCGGTTTG ACCTGCCGAC AGAGTACCGG TCGTACGTGC AATCCAAAGG TCGAGCCCGT 
GCTCCAGTCT CCAACTACAT CATGCTAGCA GACAGCGAAC GGACTAAAAC CTTCCAAGAA 
GACCTCAAGA CCTACAAGGC CATAGAGAAG ATCCTCCGAA ATAAGTGCTC GAAGTCGGCT 
GAATGCAATG ATTTTGAGCT GGAGCCTGTG ACCGATGACG ACAATGTTCT CCCACCGTAC 
GTCCTGCGCT CAGAGGACGG CGGGCCTCGT GTCACCATGA ACACTGCCAT TGGGCACGTC 
AACCGATATT GTGCGCGCTT GCCCAGTGAT CCGTTCACTC ATCTCGCTCC GAAGTGTAAA 
ACTGTAGAGA TGAACACCGG AGGGTATCGA TCCACACTCT TCCTACCTAT CAACTCACCA 
CTCAGAGTTC CTGTCACCGG GCCAGTAATG AATTGTGCAA GACTGGCTGA GAAAGCTGTC 
GCTCTTCTCT GTTGTGAAAA ACTCCACAAA ATTGGTGAAC TGGATGATCA TCTGATGCCT 
GTGGGAAAGG AAACTGTGAA GTATGAGGAG GAGCTGGACC TGCATGATGA AGAGGAGACA 
AGCGTGCCAG GAAGACCAGG CTCCACTAAA CGGAGACAGT GCTCCCCAAA AGCCATACCA 
GAGTGTCTGC GGGGTTGTTA CCCTGTGCCA GAGCAGCCTT GCTACCTTTA CGTTATTGGG 
ATGGTCCTCA CCACCCCTCT ACCTGACGAA CTCAACTTCC GGCGGAGGAA GCTGTATCCA 
CCAGAGGACA CAACCCGATG TTTTGGCATT CTGACAGCTA AACCAATACC CCGGATCCCT 
CATTTCCCTG TGTACACTCG TTCTGGTGAG GTGACCATCT CCATTGAGCT GCAGAAGTCG 
GGCTTTAGTC TAAGTGCGGA GCAGCTGGAG CTGATCACAC GTCTGCATCA GTACATCTTC 
TCCCACATCC TCCGTCTGGA AAAGCCAGCA CTAGAGTTTA AGCCTGTAGA GGCAGATTCT 
GCCTACTGTG TTCTACCTCT CAACATCGTT GAGGATTCCA ATACTTTGGA TCTGGATTTC 
AAGTTCATGG AGGATATTGA AAAGTCTGAA GCACGCATTG GCATTCCAAA CACTCAGTAC 
ACCAAGCAAA ACCCATTCAT CTTCAAATTG GAGGACTACC AGGATGCTGT CATCATTCCA 
AGGTATCGTA ATTTTGACCA GCCGCATCGG TTCTACGTGG CTGACGTTTA CACAGACCTC 
ACCCCGCTCA GTAAATTCCC CTCTCCAGAG TACGAGACTT TTGCCGAATA CTATAAAACC 
AAGTACAACC TGGACCTGTC AAATGTTAAT CAGCCCCTAC TGGATGTGGA CCACACATCT 
TCAAGATTGA ATCTTTTAAC TCCCCGGCAC CTTAATCAAA AGGGGAAAGC CCTTCCTCTC 
AGCAGTGCGG AGAAGAGAAA GGCCAAGTGG GAAAGCCTAC AAAACAAACA GATTTTAGTT 
CCGGAGCTCT GTGCCATTCA CCCCATCCCA GCCTCTTTGT GGAGGAAAGC CGTCTGCCTT 
CCCAGCATTC TCTATAGACT TCACTGCCTT CTGACGGCCG AAGAGCTGCG GTCTCAGACA 
GCCATCGATG CTGGCGTAGG GGCTCAGACC TTGCCTCCTG ATTTCAGGTA TCCTAATTTG 
GATTTCGGAT GGAAGAAATC CATCGACAGC AAGTCTTTCA TCTCCTGCCC CAGTGCATGT 
ATGGAAGAAG ACGACGATCA CTGTAAACTC GGCACAAGCT CCGATTCAAA CCACACTGCC 
CCCGAGAGCT GCAGTATGGA GGTCTCCCAG CCTCCAGAAG GCGCTCCCAA CACACCAGAT 
GAAAAGCTAG AAACCCTCAC TCTCCCTGTG ACTGATCTAA ACAAAGACTG TTTCCCAAAC 
CTCCCAAACG GCACTCAGGC CGACAGCGAC GACCTCCCTC ACAGAAGTGA CGTTTGCCAA 
TGCTCTCAGC TGGGCCCTCT GGAGAGAGAC CTATCAACAC AAACCACTAC CTCAGTTTCA 
GTGCGGCCCT CTCCAGCTGG AGAACCACAG CCATGGCCCA GCGATGAATG TACAGGCAGG 
AGCTCGGATC TCTGCGACCC ACATGTGAAG AAACCTACCT CAAAGCATTG CCCTAAATCG 
GAGACGGCGA CTAGCACCCC AGCACCTTCA GAAACGTCAT CAGAGGACTG TAGATCAGCC 
TGCGCTGGTC CAGCCTGGGA TTCGCCCAAA ACCCTGGGCC CGAACCCCGG GCTCATCCTG 
CAGGCCCTGA CTCTCTCCAA CGCCAGTGAC GGCTTCAATC TGGAACGCCT GGAGATGCTG 
GGCGACTCCT TCCTGAAACA CGCCATCACC ACATACCTGT TCTGCACTTA CCCTGACGCC 
CACGAGGGCA GACTGTCCTA CATGAGAAGC AAGAAGGTCA GCAACTGTAA CCTGTATCGG 
CTGGGGAAGA AGAAGGGTCT TCCGAGTCGA ATGGTGGTGT CCATCTTTGA CCCGCCGGTA 
AACTGGCTTC CTCCAGGCTA CGTCGTGAAT CAGGACAAAA GCAGCACAGA CAAGTGGGAC 
TCAGATGAGA ATAAAGATTT AGCGAATGGA AAAGCCAGCG ATGACGAAGA CGAGGATGAC 
GATGATGAGC CGGAAGAAGC AGAGGTCGAG CCATCTAAAG AAGATGTAAA TGTTGAAGAC 
GACCTGGAGT ATTATTACGA ACACATCCGC TTCATCGACA GCATGTTAAT CGGCTCTGGC 
GCTTTCGGCA AGAAGATCTC CCTCCAGCCC ACAGACCCCG GCTACGAATG GAAAGCTCCT 
AAAAAAGCCC ACAACTCCCA CTTCTCGCCA GACGGGGGTG CTGACGAGTT CGATTACAGC 
TCCTGGGACG CCATGTGTTA CCTGGACCCC AGTAAAGCAG GTGAGGAGGA CGATTTCGTG 
GTGGGCTTCT GGAATCCCTC TGAGGAAAAC TGCGGCACGG ACATCGGGAA ACAGTCCATC 
TCTTATGACC TTCACACGGA GCAGTGCATC GCAGATAAGA GCATAGCGGA CTGTGTGGAG 
GCTCTGCTGG GCTGCTATCT GACCAGCTGT GGTGAGCGAG CGGCTCAGCT GTTTCTCTGC 
TCTCTGGGCC TGAAGGTGCT ACCACCGGAG AAGCAGAGTT CGGGAGGCTC TGCCGAGCTC 
CAGTACGGCT GGCTGAAGAT CCCACCACGC TGCATGTTCG AACATCCTGA CGCTGAACGC 
ACGCTCAACC ACCTCATCTC TGGCTTCCTG AACTTCGAGA GCAAGATCAA CTACACGTTT 
AAGAACAAGG CGTACCTGCT GCAGGCCTTC ACACACGCCT CGTATCATTA CAACACCATT 
ACAGATTGTT ATCAGCGGTT GGAGTTTCTT GGTGATGCAA TTCTAGACTA CCTCATAACC 
AAGCACCTTT ATGAAGACCC CCGGCAGCAC TCGCCCGGCG TGCTGACCGA CCTGCGCTCT 
GCACTTGTCA ACAACACCAT ATTCGCTTCC CTGGCTGTCA AGTATGATTA CCACAAGTAC 
TTCAAAGCTG TCTCGCCAGA ACTCTTCCAC GTGATCGACG ATTTTGTGCA ATTTCAACTG 
GAGAAGAATG AAATGCAAGG AATGGATTCA GAGCTTCGCC GATCGGAGGA AGATGAAGAG 
AAAGAGGAAG ACATTGAAGT CCCCAAAGCA ATGGGAGACA TCTTTGAATC GCTAGCTGGT 
GCAATTTACA TGGATAGCGG GATGTCACTT GAGACAGTTT GGCAAGTGTA TTATCCAATG 
ATGAGGCCGC TCATAGAAAA ATTCTCCGCT AATGTCCCTC GATCCCCAGT GCGTGAACTC 
CTGGAGATGG AGCCAGAGAC AGCTAAGTTC AGCCCTGCGG AAAGAACTTA TGATGGAAAG 
GTACGGGTGA CGGTGGAAGT CGTGGGCAAA GGCAAGTTCA AAGGCGTAGG ACGCAGCTAC 
CGGATAGCCA AGTCAGCAGC CGCCCGCCGA GCCCTGCGCA GTCTCAAAGC CAACCAACCT 
CAGGTCCAAA ACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dicer1 ( XM_021467116.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021322791.1 Danio rerio dicer 1, ribonuclease type III (dicer1), transcript variant X5, mRNA.

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CloneID	ODa03830
Clone ID Related Accession (Same CDS sequence)	XM_005158665.3 , XM_005158666.3 , XM_021467115.1 , XM_005158665.4 , XM_021467114.1 , XM_021467116.1 , NM_001161453.2 , XM_005158666.4
Accession Version	XM_021467116.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5598bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGCC TACAGCTGGT GACCCCTGCC TCCTCACCCA TGGGGCCCTT CTTTGGTCTC 
CCGTGGCAAC AGGAAGCTAT CCATGACAAC ATATACACAC CAAGGAAATA TCAGGTTGAA 
CTTCTTGAAG CAGCTCTTGA ACATAATACT ATAGTCTGCT TAAATACTGG CTCAGGGAAG 
ACCTTTATCG CAGTGCTCCT AATCAAAGAA CTCTCCCACC AAATTCGTGG CGAGAATGGG 
AAGAGGACAG TTTTCCTGGT GAATGCAGCG TCATCTGTGG CTCAGCAAGC ATCTACTGTA 
AGAACACACT CTGATCTCCA AGTAGGCGAC TATATGTCAG AGGACATGAC ATCATGGCCC 
GAGGAGATGT GGAACAGAGA GATGATAGAG AATCAGGTGC TGGTGATGAC GTGCCACATC 
TTCCTGCATG TTTTAAAGAA TGGAGTCTTG CCACTATCAA AGATCAACCT GCTGGTTTTT 
GACGAATGCC ACCTTGCAAT CACTGGCCAT CCTTATCGGG AAATAATGAA GATTTGTGAA 
GGTTGCCCCA GCTGTCCTCG CATTTTGGGT CTCACTGCTT CCATTTTAAA TGGCAAATGT 
GACCCTTGTG ATTTGGAGGA GAAAATTCAG AACCTGGAGA AGATTCTTCA GAGCAATGCA 
GAAACTGCCA CTGATCTTGT GGTGCTGGAT AGGTATGCGT CGCAGCCGCG TGAGGAGGTG 
CTGGATTGTG GACAGTACCA AGATCAGAGT GGGCTCTCGG AGAGGCTTTT AAATGAGCTG 
GACGAAGCCC TCAATTTTCT AAACGATTGC AACTTGTCTG TACATAGAGA AGATAGAGAC 
CCCACGTTTA TCTCCAAACA GGTGCTGAAT GACTGTCGTG CTGTGTTGAC TGTGCTGGGC 
CCTTGGTGTG CGGATAAAGC TGCAGGAATC ATGGTTCGGG AGCTGCAGAA ATATATTAAA 
CATGAACAGG AAGAGCTAAA CCGCAAGTTC CTGTTGTTCA CCGACACCAT CCTGAGGAAA 
ATCCATGCCT TATGTGAGGA GCATTTTTCC CCAGCTTCCC TCGACCTGAA GTTTGTCACT 
CCCAAGGTCA TCAGACTTTT GGAGATTCTC CACGAGTACA AGCCTTTCGA ACGGCAGCAG 
TTTGAGAGTG TGGAGTGGTA CAACAATCGC AATCAGGACA ATTATGTTTC TTGGAGTGAT 
TCTGAAGATG ATGATGAGGA CGAAGAGGCG GAGGCAAAGG AGAAAACCGA AGCAAATTTT 
CCCTCGCCGT TCACAAATAT CCTCTGTGGA ATCATCTTTG TGGAAAGGCG ATATACAGCG 
GTTGTTTTAA ACAGACTTAT TAAGGAGGCA GGAAAGCAAG ACCCAGAGCT GGCTTACATC 
AGTAGCAACT TCATCACAGG GCACAGCATC GGCAAGAACC AGCCTCGCAA CAAGCAGATG 
GAGGTGGAGT TCAGAAAACA AGAGGAAGTC TTGAGGAAAT TCAGAGCCCA TGAAACCAAC 
TTGCTCATTG CAACCAGCAT TGTGGAGGAA GGCGTCGATA TCCCAAAGTG CAATTTGGTC 
GTTCGGTTTG ACCTGCCGAC AGAGTACCGG TCGTACGTGC AATCCAAAGG TCGAGCCCGT 
GCTCCAGTCT CCAACTACAT CATGCTAGCA GACAGCGAAC GGACTAAAAC CTTCCAAGAA 
GACCTCAAGA CCTACAAGGC CATAGAGAAG ATCCTCCGAA ATAAGTGCTC GAAGTCGGCT 
GAATGCAATG ATTTTGAGCT GGAGCCTGTG ACCGATGACG ACAATGTTCT CCCACCGTAC 
GTCCTGCGCT CAGAGGACGG CGGGCCTCGT GTCACCATGA ACACTGCCAT TGGGCACGTC 
AACCGATATT GTGCGCGCTT GCCCAGTGAT CCGTTCACTC ATCTCGCTCC GAAGTGTAAA 
ACTGTAGAGA TGAACACCGG AGGGTATCGA TCCACACTCT TCCTACCTAT CAACTCACCA 
CTCAGAGTTC CTGTCACCGG GCCAGTAATG AATTGTGCAA GACTGGCTGA GAAAGCTGTC 
GCTCTTCTCT GTTGTGAAAA ACTCCACAAA ATTGGTGAAC TGGATGATCA TCTGATGCCT 
GTGGGAAAGG AAACTGTGAA GTATGAGGAG GAGCTGGACC TGCATGATGA AGAGGAGACA 
AGCGTGCCAG GAAGACCAGG CTCCACTAAA CGGAGACAGT GCTCCCCAAA AGCCATACCA 
GAGTGTCTGC GGGGTTGTTA CCCTGTGCCA GAGCAGCCTT GCTACCTTTA CGTTATTGGG 
ATGGTCCTCA CCACCCCTCT ACCTGACGAA CTCAACTTCC GGCGGAGGAA GCTGTATCCA 
CCAGAGGACA CAACCCGATG TTTTGGCATT CTGACAGCTA AACCAATACC CCGGATCCCT 
CATTTCCCTG TGTACACTCG TTCTGGTGAG GTGACCATCT CCATTGAGCT GCAGAAGTCG 
GGCTTTAGTC TAAGTGCGGA GCAGCTGGAG CTGATCACAC GTCTGCATCA GTACATCTTC 
TCCCACATCC TCCGTCTGGA AAAGCCAGCA CTAGAGTTTA AGCCTGTAGA GGCAGATTCT 
GCCTACTGTG TTCTACCTCT CAACATCGTT GAGGATTCCA ATACTTTGGA TCTGGATTTC 
AAGTTCATGG AGGATATTGA AAAGTCTGAA GCACGCATTG GCATTCCAAA CACTCAGTAC 
ACCAAGCAAA ACCCATTCAT CTTCAAATTG GAGGACTACC AGGATGCTGT CATCATTCCA 
AGGTATCGTA ATTTTGACCA GCCGCATCGG TTCTACGTGG CTGACGTTTA CACAGACCTC 
ACCCCGCTCA GTAAATTCCC CTCTCCAGAG TACGAGACTT TTGCCGAATA CTATAAAACC 
AAGTACAACC TGGACCTGTC AAATGTTAAT CAGCCCCTAC TGGATGTGGA CCACACATCT 
TCAAGATTGA ATCTTTTAAC TCCCCGGCAC CTTAATCAAA AGGGGAAAGC CCTTCCTCTC 
AGCAGTGCGG AGAAGAGAAA GGCCAAGTGG GAAAGCCTAC AAAACAAACA GATTTTAGTT 
CCGGAGCTCT GTGCCATTCA CCCCATCCCA GCCTCTTTGT GGAGGAAAGC CGTCTGCCTT 
CCCAGCATTC TCTATAGACT TCACTGCCTT CTGACGGCCG AAGAGCTGCG GTCTCAGACA 
GCCATCGATG CTGGCGTAGG GGCTCAGACC TTGCCTCCTG ATTTCAGGTA TCCTAATTTG 
GATTTCGGAT GGAAGAAATC CATCGACAGC AAGTCTTTCA TCTCCTGCCC CAGTGCATGT 
ATGGAAGAAG ACGACGATCA CTGTAAACTC GGCACAAGCT CCGATTCAAA CCACACTGCC 
CCCGAGAGCT GCAGTATGGA GGTCTCCCAG CCTCCAGAAG GCGCTCCCAA CACACCAGAT 
GAAAAGCTAG AAACCCTCAC TCTCCCTGTG ACTGATCTAA ACAAAGACTG TTTCCCAAAC 
CTCCCAAACG GCACTCAGGC CGACAGCGAC GACCTCCCTC ACAGAAGTGA CGTTTGCCAA 
TGCTCTCAGC TGGGCCCTCT GGAGAGAGAC CTATCAACAC AAACCACTAC CTCAGTTTCA 
GTGCGGCCCT CTCCAGCTGG AGAACCACAG CCATGGCCCA GCGATGAATG TACAGGCAGG 
AGCTCGGATC TCTGCGACCC ACATGTGAAG AAACCTACCT CAAAGCATTG CCCTAAATCG 
GAGACGGCGA CTAGCACCCC AGCACCTTCA GAAACGTCAT CAGAGGACTG TAGATCAGCC 
TGCGCTGGTC CAGCCTGGGA TTCGCCCAAA ACCCTGGGCC CGAACCCCGG GCTCATCCTG 
CAGGCCCTGA CTCTCTCCAA CGCCAGTGAC GGCTTCAATC TGGAACGCCT GGAGATGCTG 
GGCGACTCCT TCCTGAAACA CGCCATCACC ACATACCTGT TCTGCACTTA CCCTGACGCC 
CACGAGGGCA GACTGTCCTA CATGAGAAGC AAGAAGGTCA GCAACTGTAA CCTGTATCGG 
CTGGGGAAGA AGAAGGGTCT TCCGAGTCGA ATGGTGGTGT CCATCTTTGA CCCGCCGGTA 
AACTGGCTTC CTCCAGGCTA CGTCGTGAAT CAGGACAAAA GCAGCACAGA CAAGTGGGAC 
TCAGATGAGA ATAAAGATTT AGCGAATGGA AAAGCCAGCG ATGACGAAGA CGAGGATGAC 
GATGATGAGC CGGAAGAAGC AGAGGTCGAG CCATCTAAAG AAGATGTAAA TGTTGAAGAC 
GACCTGGAGT ATTATTACGA ACACATCCGC TTCATCGACA GCATGTTAAT CGGCTCTGGC 
GCTTTCGGCA AGAAGATCTC CCTCCAGCCC ACAGACCCCG GCTACGAATG GAAAGCTCCT 
AAAAAAGCCC ACAACTCCCA CTTCTCGCCA GACGGGGGTG CTGACGAGTT CGATTACAGC 
TCCTGGGACG CCATGTGTTA CCTGGACCCC AGTAAAGCAG GTGAGGAGGA CGATTTCGTG 
GTGGGCTTCT GGAATCCCTC TGAGGAAAAC TGCGGCACGG ACATCGGGAA ACAGTCCATC 
TCTTATGACC TTCACACGGA GCAGTGCATC GCAGATAAGA GCATAGCGGA CTGTGTGGAG 
GCTCTGCTGG GCTGCTATCT GACCAGCTGT GGTGAGCGAG CGGCTCAGCT GTTTCTCTGC 
TCTCTGGGCC TGAAGGTGCT ACCACCGGAG AAGCAGAGTT CGGGAGGCTC TGCCGAGCTC 
CAGTACGGCT GGCTGAAGAT CCCACCACGC TGCATGTTCG AACATCCTGA CGCTGAACGC 
ACGCTCAACC ACCTCATCTC TGGCTTCCTG AACTTCGAGA GCAAGATCAA CTACACGTTT 
AAGAACAAGG CGTACCTGCT GCAGGCCTTC ACACACGCCT CGTATCATTA CAACACCATT 
ACAGATTGTT ATCAGCGGTT GGAGTTTCTT GGTGATGCAA TTCTAGACTA CCTCATAACC 
AAGCACCTTT ATGAAGACCC CCGGCAGCAC TCGCCCGGCG TGCTGACCGA CCTGCGCTCT 
GCACTTGTCA ACAACACCAT ATTCGCTTCC CTGGCTGTCA AGTATGATTA CCACAAGTAC 
TTCAAAGCTG TCTCGCCAGA ACTCTTCCAC GTGATCGACG ATTTTGTGCA ATTTCAACTG 
GAGAAGAATG AAATGCAAGG AATGGATTCA GAGCTTCGCC GATCGGAGGA AGATGAAGAG 
AAAGAGGAAG ACATTGAAGT CCCCAAAGCA ATGGGAGACA TCTTTGAATC GCTAGCTGGT 
GCAATTTACA TGGATAGCGG GATGTCACTT GAGACAGTTT GGCAAGTGTA TTATCCAATG 
ATGAGGCCGC TCATAGAAAA ATTCTCCGCT AATGTCCCTC GATCCCCAGT GCGTGAACTC 
CTGGAGATGG AGCCAGAGAC AGCTAAGTTC AGCCCTGCGG AAAGAACTTA TGATGGAAAG 
GTACGGGTGA CGGTGGAAGT CGTGGGCAAA GGCAAGTTCA AAGGCGTAGG ACGCAGCTAC 
CGGATAGCCA AGTCAGCAGC CGCCCGCCGA GCCCTGCGCA GTCTCAAAGC CAACCAACCT 
CAGGTCCAAA ACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021322791.1
CDS	375..5972
Translation	MAGLQLVTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALEHNTIVCLNTGSGKTFIAVLLIKELSHQIRGENGKRTVFLVNAASSVAQQASTVRTHSDLQVGDYMSEDMTSWPEEMWNREMIENQVLVMTCHIFLHVLKNGVLPLSKINLLVFDECHLAITGHPYREIMKICEGCPSCPRILGLTASILNGKCDPCDLEEKIQNLEKILQSNAETATDLVVLDRYASQPREEVLDCGQYQDQSGLSERLLNELDEALNFLNDCNLSVHREDRDPTFISKQVLNDCRAVLTVLGPWCADKAAGIMVRELQKYIKHEQEELNRKFLLFTDTILRKIHALCEEHFSPASLDLKFVTPKVIRLLEILHEYKPFERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEAEAKEKTEANFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHSIGKNQPRNKQMEVEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPVSNYIMLADSERTKTFQEDLKTYKAIEKILRNKCSKSAECNDFELEPVTDDDNVLPPYVLRSEDGGPRVTMNTAIGHVNRYCARLPSDPFTHLAPKCKTVEMNTGGYRSTLFLPINSPLRVPVTGPVMNCARLAEKAVALLCCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCSPKAIPECLRGCYPVPEQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPRIPHFPVYTRSGEVTISIELQKSGFSLSAEQLELITRLHQYIFSHILRLEKPALEFKPVEADSAYCVLPLNIVEDSNTLDLDFKFMEDIEKSEARIGIPNTQYTKQNPFIFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLSNVNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRSQTAIDAGVGAQTLPPDFRYPNLDFGWKKSIDSKSFISCPSACMEEDDDHCKLGTSSDSNHTAPESCSMEVSQPPEGAPNTPDEKLETLTLPVTDLNKDCFPNLPNGTQADSDDLPHRSDVCQCSQLGPLERDLSTQTTTSVSVRPSPAGEPQPWPSDECTGRSSDLCDPHVKKPTSKHCPKSETATSTPAPSETSSEDCRSACAGPAWDSPKTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSSTDKWDSDENKDLANGKASDDEDEDDDDEPEEAEVEPSKEDVNVEDDLEYYYEHIRFIDSMLIGSGAFGKKISLQPTDPGYEWKAPKKAHNSHFSPDGGADEFDYSSWDAMCYLDPSKAGEEDDFVVGFWNPSEENCGTDIGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPPEKQSSGGSAELQYGWLKIPPRCMFEHPDAERTLNHLISGFLNFESKINYTFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVQNN

Target ORF information:

RefSeq Version	XM_021467116.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio dicer 1, ribonuclease type III (dicer1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021467116.1

ORF Insert Sequence:

ATGGCTGGCC TACAGCTGGT GACCCCTGCC TCCTCACCCA TGGGGCCCTT CTTTGGTCTC 
CCGTGGCAAC AGGAAGCTAT CCATGACAAC ATATACACAC CAAGGAAATA TCAGGTTGAA 
CTTCTTGAAG CAGCTCTTGA ACATAATACT ATAGTCTGCT TAAATACTGG CTCAGGGAAG 
ACCTTTATCG CAGTGCTCCT AATCAAAGAA CTCTCCCACC AAATTCGTGG CGAGAATGGG 
AAGAGGACAG TTTTCCTGGT GAATGCAGCG TCATCTGTGG CTCAGCAAGC ATCTACTGTA 
AGAACACACT CTGATCTCCA AGTAGGCGAC TATATGTCAG AGGACATGAC ATCATGGCCC 
GAGGAGATGT GGAACAGAGA GATGATAGAG AATCAGGTGC TGGTGATGAC GTGCCACATC 
TTCCTGCATG TTTTAAAGAA TGGAGTCTTG CCACTATCAA AGATCAACCT GCTGGTTTTT 
GACGAATGCC ACCTTGCAAT CACTGGCCAT CCTTATCGGG AAATAATGAA GATTTGTGAA 
GGTTGCCCCA GCTGTCCTCG CATTTTGGGT CTCACTGCTT CCATTTTAAA TGGCAAATGT 
GACCCTTGTG ATTTGGAGGA GAAAATTCAG AACCTGGAGA AGATTCTTCA GAGCAATGCA 
GAAACTGCCA CTGATCTTGT GGTGCTGGAT AGGTATGCGT CGCAGCCGCG TGAGGAGGTG 
CTGGATTGTG GACAGTACCA AGATCAGAGT GGGCTCTCGG AGAGGCTTTT AAATGAGCTG 
GACGAAGCCC TCAATTTTCT AAACGATTGC AACTTGTCTG TACATAGAGA AGATAGAGAC 
CCCACGTTTA TCTCCAAACA GGTGCTGAAT GACTGTCGTG CTGTGTTGAC TGTGCTGGGC 
CCTTGGTGTG CGGATAAAGC TGCAGGAATC ATGGTTCGGG AGCTGCAGAA ATATATTAAA 
CATGAACAGG AAGAGCTAAA CCGCAAGTTC CTGTTGTTCA CCGACACCAT CCTGAGGAAA 
ATCCATGCCT TATGTGAGGA GCATTTTTCC CCAGCTTCCC TCGACCTGAA GTTTGTCACT 
CCCAAGGTCA TCAGACTTTT GGAGATTCTC CACGAGTACA AGCCTTTCGA ACGGCAGCAG 
TTTGAGAGTG TGGAGTGGTA CAACAATCGC AATCAGGACA ATTATGTTTC TTGGAGTGAT 
TCTGAAGATG ATGATGAGGA CGAAGAGGCG GAGGCAAAGG AGAAAACCGA AGCAAATTTT 
CCCTCGCCGT TCACAAATAT CCTCTGTGGA ATCATCTTTG TGGAAAGGCG ATATACAGCG 
GTTGTTTTAA ACAGACTTAT TAAGGAGGCA GGAAAGCAAG ACCCAGAGCT GGCTTACATC 
AGTAGCAACT TCATCACAGG GCACAGCATC GGCAAGAACC AGCCTCGCAA CAAGCAGATG 
GAGGTGGAGT TCAGAAAACA AGAGGAAGTC TTGAGGAAAT TCAGAGCCCA TGAAACCAAC 
TTGCTCATTG CAACCAGCAT TGTGGAGGAA GGCGTCGATA TCCCAAAGTG CAATTTGGTC 
GTTCGGTTTG ACCTGCCGAC AGAGTACCGG TCGTACGTGC AATCCAAAGG TCGAGCCCGT 
GCTCCAGTCT CCAACTACAT CATGCTAGCA GACAGCGAAC GGACTAAAAC CTTCCAAGAA 
GACCTCAAGA CCTACAAGGC CATAGAGAAG ATCCTCCGAA ATAAGTGCTC GAAGTCGGCT 
GAATGCAATG ATTTTGAGCT GGAGCCTGTG ACCGATGACG ACAATGTTCT CCCACCGTAC 
GTCCTGCGCT CAGAGGACGG CGGGCCTCGT GTCACCATGA ACACTGCCAT TGGGCACGTC 
AACCGATATT GTGCGCGCTT GCCCAGTGAT CCGTTCACTC ATCTCGCTCC GAAGTGTAAA 
ACTGTAGAGA TGAACACCGG AGGGTATCGA TCCACACTCT TCCTACCTAT CAACTCACCA 
CTCAGAGTTC CTGTCACCGG GCCAGTAATG AATTGTGCAA GACTGGCTGA GAAAGCTGTC 
GCTCTTCTCT GTTGTGAAAA ACTCCACAAA ATTGGTGAAC TGGATGATCA TCTGATGCCT 
GTGGGAAAGG AAACTGTGAA GTATGAGGAG GAGCTGGACC TGCATGATGA AGAGGAGACA 
AGCGTGCCAG GAAGACCAGG CTCCACTAAA CGGAGACAGT GCTCCCCAAA AGCCATACCA 
GAGTGTCTGC GGGGTTGTTA CCCTGTGCCA GAGCAGCCTT GCTACCTTTA CGTTATTGGG 
ATGGTCCTCA CCACCCCTCT ACCTGACGAA CTCAACTTCC GGCGGAGGAA GCTGTATCCA 
CCAGAGGACA CAACCCGATG TTTTGGCATT CTGACAGCTA AACCAATACC CCGGATCCCT 
CATTTCCCTG TGTACACTCG TTCTGGTGAG GTGACCATCT CCATTGAGCT GCAGAAGTCG 
GGCTTTAGTC TAAGTGCGGA GCAGCTGGAG CTGATCACAC GTCTGCATCA GTACATCTTC 
TCCCACATCC TCCGTCTGGA AAAGCCAGCA CTAGAGTTTA AGCCTGTAGA GGCAGATTCT 
GCCTACTGTG TTCTACCTCT CAACATCGTT GAGGATTCCA ATACTTTGGA TCTGGATTTC 
AAGTTCATGG AGGATATTGA AAAGTCTGAA GCACGCATTG GCATTCCAAA CACTCAGTAC 
ACCAAGCAAA ACCCATTCAT CTTCAAATTG GAGGACTACC AGGATGCTGT CATCATTCCA 
AGGTATCGTA ATTTTGACCA GCCGCATCGG TTCTACGTGG CTGACGTTTA CACAGACCTC 
ACCCCGCTCA GTAAATTCCC CTCTCCAGAG TACGAGACTT TTGCCGAATA CTATAAAACC 
AAGTACAACC TGGACCTGTC AAATGTTAAT CAGCCCCTAC TGGATGTGGA CCACACATCT 
TCAAGATTGA ATCTTTTAAC TCCCCGGCAC CTTAATCAAA AGGGGAAAGC CCTTCCTCTC 
AGCAGTGCGG AGAAGAGAAA GGCCAAGTGG GAAAGCCTAC AAAACAAACA GATTTTAGTT 
CCGGAGCTCT GTGCCATTCA CCCCATCCCA GCCTCTTTGT GGAGGAAAGC CGTCTGCCTT 
CCCAGCATTC TCTATAGACT TCACTGCCTT CTGACGGCCG AAGAGCTGCG GTCTCAGACA 
GCCATCGATG CTGGCGTAGG GGCTCAGACC TTGCCTCCTG ATTTCAGGTA TCCTAATTTG 
GATTTCGGAT GGAAGAAATC CATCGACAGC AAGTCTTTCA TCTCCTGCCC CAGTGCATGT 
ATGGAAGAAG ACGACGATCA CTGTAAACTC GGCACAAGCT CCGATTCAAA CCACACTGCC 
CCCGAGAGCT GCAGTATGGA GGTCTCCCAG CCTCCAGAAG GCGCTCCCAA CACACCAGAT 
GAAAAGCTAG AAACCCTCAC TCTCCCTGTG ACTGATCTAA ACAAAGACTG TTTCCCAAAC 
CTCCCAAACG GCACTCAGGC CGACAGCGAC GACCTCCCTC ACAGAAGTGA CGTTTGCCAA 
TGCTCTCAGC TGGGCCCTCT GGAGAGAGAC CTATCAACAC AAACCACTAC CTCAGTTTCA 
GTGCGGCCCT CTCCAGCTGG AGAACCACAG CCATGGCCCA GCGATGAATG TACAGGCAGG 
AGCTCGGATC TCTGCGACCC ACATGTGAAG AAACCTACCT CAAAGCATTG CCCTAAATCG 
GAGACGGCGA CTAGCACCCC AGCACCTTCA GAAACGTCAT CAGAGGACTG TAGATCAGCC 
TGCGCTGGTC CAGCCTGGGA TTCGCCCAAA ACCCTGGGCC CGAACCCCGG GCTCATCCTG 
CAGGCCCTGA CTCTCTCCAA CGCCAGTGAC GGCTTCAATC TGGAACGCCT GGAGATGCTG 
GGCGACTCCT TCCTGAAACA CGCCATCACC ACATACCTGT TCTGCACTTA CCCTGACGCC 
CACGAGGGCA GACTGTCCTA CATGAGAAGC AAGAAGGTCA GCAACTGTAA CCTGTATCGG 
CTGGGGAAGA AGAAGGGTCT TCCGAGTCGA ATGGTGGTGT CCATCTTTGA CCCGCCGGTA 
AACTGGCTTC CTCCAGGCTA CGTCGTGAAT CAGGACAAAA GCAGCACAGA CAAGTGGGAC 
TCAGATGAGA ATAAAGATTT AGCGAATGGA AAAGCCAGCG ATGACGAAGA CGAGGATGAC 
GATGATGAGC CGGAAGAAGC AGAGGTCGAG CCATCTAAAG AAGATGTAAA TGTTGAAGAC 
GACCTGGAGT ATTATTACGA ACACATCCGC TTCATCGACA GCATGTTAAT CGGCTCTGGC 
GCTTTCGGCA AGAAGATCTC CCTCCAGCCC ACAGACCCCG GCTACGAATG GAAAGCTCCT 
AAAAAAGCCC ACAACTCCCA CTTCTCGCCA GACGGGGGTG CTGACGAGTT CGATTACAGC 
TCCTGGGACG CCATGTGTTA CCTGGACCCC AGTAAAGCAG GTGAGGAGGA CGATTTCGTG 
GTGGGCTTCT GGAATCCCTC TGAGGAAAAC TGCGGCACGG ACATCGGGAA ACAGTCCATC 
TCTTATGACC TTCACACGGA GCAGTGCATC GCAGATAAGA GCATAGCGGA CTGTGTGGAG 
GCTCTGCTGG GCTGCTATCT GACCAGCTGT GGTGAGCGAG CGGCTCAGCT GTTTCTCTGC 
TCTCTGGGCC TGAAGGTGCT ACCACCGGAG AAGCAGAGTT CGGGAGGCTC TGCCGAGCTC 
CAGTACGGCT GGCTGAAGAT CCCACCACGC TGCATGTTCG AACATCCTGA CGCTGAACGC 
ACGCTCAACC ACCTCATCTC TGGCTTCCTG AACTTCGAGA GCAAGATCAA CTACACGTTT 
AAGAACAAGG CGTACCTGCT GCAGGCCTTC ACACACGCCT CGTATCATTA CAACACCATT 
ACAGATTGTT ATCAGCGGTT GGAGTTTCTT GGTGATGCAA TTCTAGACTA CCTCATAACC 
AAGCACCTTT ATGAAGACCC CCGGCAGCAC TCGCCCGGCG TGCTGACCGA CCTGCGCTCT 
GCACTTGTCA ACAACACCAT ATTCGCTTCC CTGGCTGTCA AGTATGATTA CCACAAGTAC 
TTCAAAGCTG TCTCGCCAGA ACTCTTCCAC GTGATCGACG ATTTTGTGCA ATTTCAACTG 
GAGAAGAATG AAATGCAAGG AATGGATTCA GAGCTTCGCC GATCGGAGGA AGATGAAGAG 
AAAGAGGAAG ACATTGAAGT CCCCAAAGCA ATGGGAGACA TCTTTGAATC GCTAGCTGGT 
GCAATTTACA TGGATAGCGG GATGTCACTT GAGACAGTTT GGCAAGTGTA TTATCCAATG 
ATGAGGCCGC TCATAGAAAA ATTCTCCGCT AATGTCCCTC GATCCCCAGT GCGTGAACTC 
CTGGAGATGG AGCCAGAGAC AGCTAAGTTC AGCCCTGCGG AAAGAACTTA TGATGGAAAG 
GTACGGGTGA CGGTGGAAGT CGTGGGCAAA GGCAAGTTCA AAGGCGTAGG ACGCAGCTAC 
CGGATAGCCA AGTCAGCAGC CGCCCGCCGA GCCCTGCGCA GTCTCAAAGC CAACCAACCT 
CAGGTCCAAA ACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dicer1 ( XM_005158666.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005158723.1 Danio rerio dicer 1, ribonuclease type III (dicer1), transcript variant X1, mRNA.

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CloneID	ODa03830
Clone ID Related Accession (Same CDS sequence)	XM_005158665.3 , XM_005158666.3 , XM_021467115.1 , XM_005158665.4 , XM_021467114.1 , XM_021467116.1 , NM_001161453.2 , XM_005158666.4
Accession Version	XM_005158666.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5598bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005158666.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGCC TACAGCTGGT GACCCCTGCC TCCTCACCCA TGGGGCCCTT CTTTGGTCTC 
CCGTGGCAAC AGGAAGCTAT CCATGACAAC ATATACACAC CAAGGAAATA TCAGGTTGAA 
CTTCTTGAAG CAGCTCTTGA ACATAATACT ATAGTCTGCT TAAATACTGG CTCAGGGAAG 
ACCTTTATCG CAGTGCTCCT AATCAAAGAA CTCTCCCACC AAATTCGTGG CGAGAATGGG 
AAGAGGACAG TTTTCCTGGT GAATGCAGCG TCATCTGTGG CTCAGCAAGC ATCTACTGTA 
AGAACACACT CTGATCTCCA AGTAGGCGAC TATATGTCAG AGGACATGAC ATCATGGCCC 
GAGGAGATGT GGAACAGAGA GATGATAGAG AATCAGGTGC TGGTGATGAC GTGCCACATC 
TTCCTGCATG TTTTAAAGAA TGGAGTCTTG CCACTATCAA AGATCAACCT GCTGGTTTTT 
GACGAATGCC ACCTTGCAAT CACTGGCCAT CCTTATCGGG AAATAATGAA GATTTGTGAA 
GGTTGCCCCA GCTGTCCTCG CATTTTGGGT CTCACTGCTT CCATTTTAAA TGGCAAATGT 
GACCCTTGTG ATTTGGAGGA GAAAATTCAG AACCTGGAGA AGATTCTTCA GAGCAATGCA 
GAAACTGCCA CTGATCTTGT GGTGCTGGAT AGGTATGCGT CGCAGCCGCG TGAGGAGGTG 
CTGGATTGTG GACAGTACCA AGATCAGAGT GGGCTCTCGG AGAGGCTTTT AAATGAGCTG 
GACGAAGCCC TCAATTTTCT AAACGATTGC AACTTGTCTG TACATAGAGA AGATAGAGAC 
CCCACGTTTA TCTCCAAACA GGTGCTGAAT GACTGTCGTG CTGTGTTGAC TGTGCTGGGC 
CCTTGGTGTG CGGATAAAGC TGCAGGAATC ATGGTTCGGG AGCTGCAGAA ATATATTAAA 
CATGAACAGG AAGAGCTAAA CCGCAAGTTC CTGTTGTTCA CCGACACCAT CCTGAGGAAA 
ATCCATGCCT TATGTGAGGA GCATTTTTCC CCAGCTTCCC TCGACCTGAA GTTTGTCACT 
CCCAAGGTCA TCAGACTTTT GGAGATTCTC CACGAGTACA AGCCTTTCGA ACGGCAGCAG 
TTTGAGAGTG TGGAGTGGTA CAACAATCGC AATCAGGACA ATTATGTTTC TTGGAGTGAT 
TCTGAAGATG ATGATGAGGA CGAAGAGGCG GAGGCAAAGG AGAAAACCGA AGCAAATTTT 
CCCTCGCCGT TCACAAATAT CCTCTGTGGA ATCATCTTTG TGGAAAGGCG ATATACAGCG 
GTTGTTTTAA ACAGACTTAT TAAGGAGGCA GGAAAGCAAG ACCCAGAGCT GGCTTACATC 
AGTAGCAACT TCATCACAGG GCACAGCATC GGCAAGAACC AGCCTCGCAA CAAGCAGATG 
GAGGTGGAGT TCAGAAAACA AGAGGAAGTC TTGAGGAAAT TCAGAGCCCA TGAAACCAAC 
TTGCTCATTG CAACCAGCAT TGTGGAGGAA GGCGTCGATA TCCCAAAGTG CAATTTGGTC 
GTTCGGTTTG ACCTGCCGAC AGAGTACCGG TCGTACGTGC AATCCAAAGG TCGAGCCCGT 
GCTCCAGTCT CCAACTACAT CATGCTAGCA GACAGCGAAC GGACTAAAAC CTTCCAAGAA 
GACCTCAAGA CCTACAAGGC CATAGAGAAG ATCCTCCGAA ATAAGTGCTC GAAGTCGGCT 
GAATGCAATG ATTTTGAGCT GGAGCCTGTG ACCGATGACG ACAATGTTCT CCCACCGTAC 
GTCCTGCGCT CAGAGGACGG CGGGCCTCGT GTCACCATGA ACACTGCCAT TGGGCACGTC 
AACCGATATT GTGCGCGCTT GCCCAGTGAT CCGTTCACTC ATCTCGCTCC GAAGTGTAAA 
ACTGTAGAGA TGAACACCGG AGGGTATCGA TCCACACTCT TCCTACCTAT CAACTCACCA 
CTCAGAGTTC CTGTCACCGG GCCAGTAATG AATTGTGCAA GACTGGCTGA GAAAGCTGTC 
GCTCTTCTCT GTTGTGAAAA ACTCCACAAA ATTGGTGAAC TGGATGATCA TCTGATGCCT 
GTGGGAAAGG AAACTGTGAA GTATGAGGAG GAGCTGGACC TGCATGATGA AGAGGAGACA 
AGCGTGCCAG GAAGACCAGG CTCCACTAAA CGGAGACAGT GCTCCCCAAA AGCCATACCA 
GAGTGTCTGC GGGGTTGTTA CCCTGTGCCA GAGCAGCCTT GCTACCTTTA CGTTATTGGG 
ATGGTCCTCA CCACCCCTCT ACCTGACGAA CTCAACTTCC GGCGGAGGAA GCTGTATCCA 
CCAGAGGACA CAACCCGATG TTTTGGCATT CTGACAGCTA AACCAATACC CCGGATCCCT 
CATTTCCCTG TGTACACTCG TTCTGGTGAG GTGACCATCT CCATTGAGCT GCAGAAGTCG 
GGCTTTAGTC TAAGTGCGGA GCAGCTGGAG CTGATCACAC GTCTGCATCA GTACATCTTC 
TCCCACATCC TCCGTCTGGA AAAGCCAGCA CTAGAGTTTA AGCCTGTAGA GGCAGATTCT 
GCCTACTGTG TTCTACCTCT CAACATCGTT GAGGATTCCA ATACTTTGGA TCTGGATTTC 
AAGTTCATGG AGGATATTGA AAAGTCTGAA GCACGCATTG GCATTCCAAA CACTCAGTAC 
ACCAAGCAAA ACCCATTCAT CTTCAAATTG GAGGACTACC AGGATGCTGT CATCATTCCA 
AGGTATCGTA ATTTTGACCA GCCGCATCGG TTCTACGTGG CTGACGTTTA CACAGACCTC 
ACCCCGCTCA GTAAATTCCC CTCTCCAGAG TACGAGACTT TTGCCGAATA CTATAAAACC 
AAGTACAACC TGGACCTGTC AAATGTTAAT CAGCCCCTAC TGGATGTGGA CCACACATCT 
TCAAGATTGA ATCTTTTAAC TCCCCGGCAC CTTAATCAAA AGGGGAAAGC CCTTCCTCTC 
AGCAGTGCGG AGAAGAGAAA GGCCAAGTGG GAAAGCCTAC AAAACAAACA GATTTTAGTT 
CCGGAGCTCT GTGCCATTCA CCCCATCCCA GCCTCTTTGT GGAGGAAAGC CGTCTGCCTT 
CCCAGCATTC TCTATAGACT TCACTGCCTT CTGACGGCCG AAGAGCTGCG GTCTCAGACA 
GCCATCGATG CTGGCGTAGG GGCTCAGACC TTGCCTCCTG ATTTCAGGTA TCCTAATTTG 
GATTTCGGAT GGAAGAAATC CATCGACAGC AAGTCTTTCA TCTCCTGCCC CAGTGCATGT 
ATGGAAGAAG ACGACGATCA CTGTAAACTC GGCACAAGCT CCGATTCAAA CCACACTGCC 
CCCGAGAGCT GCAGTATGGA GGTCTCCCAG CCTCCAGAAG GCGCTCCCAA CACACCAGAT 
GAAAAGCTAG AAACCCTCAC TCTCCCTGTG ACTGATCTAA ACAAAGACTG TTTCCCAAAC 
CTCCCAAACG GCACTCAGGC CGACAGCGAC GACCTCCCTC ACAGAAGTGA CGTTTGCCAA 
TGCTCTCAGC TGGGCCCTCT GGAGAGAGAC CTATCAACAC AAACCACTAC CTCAGTTTCA 
GTGCGGCCCT CTCCAGCTGG AGAACCACAG CCATGGCCCA GCGATGAATG TACAGGCAGG 
AGCTCGGATC TCTGCGACCC ACATGTGAAG AAACCTACCT CAAAGCATTG CCCTAAATCG 
GAGACGGCGA CTAGCACCCC AGCACCTTCA GAAACGTCAT CAGAGGACTG TAGATCAGCC 
TGCGCTGGTC CAGCCTGGGA TTCGCCCAAA ACCCTGGGCC CGAACCCCGG GCTCATCCTG 
CAGGCCCTGA CTCTCTCCAA CGCCAGTGAC GGCTTCAATC TGGAACGCCT GGAGATGCTG 
GGCGACTCCT TCCTGAAACA CGCCATCACC ACATACCTGT TCTGCACTTA CCCTGACGCC 
CACGAGGGCA GACTGTCCTA CATGAGAAGC AAGAAGGTCA GCAACTGTAA CCTGTATCGG 
CTGGGGAAGA AGAAGGGTCT TCCGAGTCGA ATGGTGGTGT CCATCTTTGA CCCGCCGGTA 
AACTGGCTTC CTCCAGGCTA CGTCGTGAAT CAGGACAAAA GCAGCACAGA CAAGTGGGAC 
TCAGATGAGA ATAAAGATTT AGCGAATGGA AAAGCCAGCG ATGACGAAGA CGAGGATGAC 
GATGATGAGC CGGAAGAAGC AGAGGTCGAG CCATCTAAAG AAGATGTAAA TGTTGAAGAC 
GACCTGGAGT ATTATTACGA ACACATCCGC TTCATCGACA GCATGTTAAT CGGCTCTGGC 
GCTTTCGGCA AGAAGATCTC CCTCCAGCCC ACAGACCCCG GCTACGAATG GAAAGCTCCT 
AAAAAAGCCC ACAACTCCCA CTTCTCGCCA GACGGGGGTG CTGACGAGTT CGATTACAGC 
TCCTGGGACG CCATGTGTTA CCTGGACCCC AGTAAAGCAG GTGAGGAGGA CGATTTCGTG 
GTGGGCTTCT GGAATCCCTC TGAGGAAAAC TGCGGCACGG ACATCGGGAA ACAGTCCATC 
TCTTATGACC TTCACACGGA GCAGTGCATC GCAGATAAGA GCATAGCGGA CTGTGTGGAG 
GCTCTGCTGG GCTGCTATCT GACCAGCTGT GGTGAGCGAG CGGCTCAGCT GTTTCTCTGC 
TCTCTGGGCC TGAAGGTGCT ACCACCGGAG AAGCAGAGTT CGGGAGGCTC TGCCGAGCTC 
CAGTACGGCT GGCTGAAGAT CCCACCACGC TGCATGTTCG AACATCCTGA CGCTGAACGC 
ACGCTCAACC ACCTCATCTC TGGCTTCCTG AACTTCGAGA GCAAGATCAA CTACACGTTT 
AAGAACAAGG CGTACCTGCT GCAGGCCTTC ACACACGCCT CGTATCATTA CAACACCATT 
ACAGATTGTT ATCAGCGGTT GGAGTTTCTT GGTGATGCAA TTCTAGACTA CCTCATAACC 
AAGCACCTTT ATGAAGACCC CCGGCAGCAC TCGCCCGGCG TGCTGACCGA CCTGCGCTCT 
GCACTTGTCA ACAACACCAT ATTCGCTTCC CTGGCTGTCA AGTATGATTA CCACAAGTAC 
TTCAAAGCTG TCTCGCCAGA ACTCTTCCAC GTGATCGACG ATTTTGTGCA ATTTCAACTG 
GAGAAGAATG AAATGCAAGG AATGGATTCA GAGCTTCGCC GATCGGAGGA AGATGAAGAG 
AAAGAGGAAG ACATTGAAGT CCCCAAAGCA ATGGGAGACA TCTTTGAATC GCTAGCTGGT 
GCAATTTACA TGGATAGCGG GATGTCACTT GAGACAGTTT GGCAAGTGTA TTATCCAATG 
ATGAGGCCGC TCATAGAAAA ATTCTCCGCT AATGTCCCTC GATCCCCAGT GCGTGAACTC 
CTGGAGATGG AGCCAGAGAC AGCTAAGTTC AGCCCTGCGG AAAGAACTTA TGATGGAAAG 
GTACGGGTGA CGGTGGAAGT CGTGGGCAAA GGCAAGTTCA AAGGCGTAGG ACGCAGCTAC 
CGGATAGCCA AGTCAGCAGC CGCCCGCCGA GCCCTGCGCA GTCTCAAAGC CAACCAACCT 
CAGGTCCAAA ACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158723.1
CDS	382..5979
Translation	MAGLQLVTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALEHNTIVCLNTGSGKTFIAVLLIKELSHQIRGENGKRTVFLVNAASSVAQQASTVRTHSDLQVGDYMSEDMTSWPEEMWNREMIENQVLVMTCHIFLHVLKNGVLPLSKINLLVFDECHLAITGHPYREIMKICEGCPSCPRILGLTASILNGKCDPCDLEEKIQNLEKILQSNAETATDLVVLDRYASQPREEVLDCGQYQDQSGLSERLLNELDEALNFLNDCNLSVHREDRDPTFISKQVLNDCRAVLTVLGPWCADKAAGIMVRELQKYIKHEQEELNRKFLLFTDTILRKIHALCEEHFSPASLDLKFVTPKVIRLLEILHEYKPFERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEAEAKEKTEANFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHSIGKNQPRNKQMEVEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPVSNYIMLADSERTKTFQEDLKTYKAIEKILRNKCSKSAECNDFELEPVTDDDNVLPPYVLRSEDGGPRVTMNTAIGHVNRYCARLPSDPFTHLAPKCKTVEMNTGGYRSTLFLPINSPLRVPVTGPVMNCARLAEKAVALLCCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCSPKAIPECLRGCYPVPEQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPRIPHFPVYTRSGEVTISIELQKSGFSLSAEQLELITRLHQYIFSHILRLEKPALEFKPVEADSAYCVLPLNIVEDSNTLDLDFKFMEDIEKSEARIGIPNTQYTKQNPFIFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLSNVNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRSQTAIDAGVGAQTLPPDFRYPNLDFGWKKSIDSKSFISCPSACMEEDDDHCKLGTSSDSNHTAPESCSMEVSQPPEGAPNTPDEKLETLTLPVTDLNKDCFPNLPNGTQADSDDLPHRSDVCQCSQLGPLERDLSTQTTTSVSVRPSPAGEPQPWPSDECTGRSSDLCDPHVKKPTSKHCPKSETATSTPAPSETSSEDCRSACAGPAWDSPKTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSSTDKWDSDENKDLANGKASDDEDEDDDDEPEEAEVEPSKEDVNVEDDLEYYYEHIRFIDSMLIGSGAFGKKISLQPTDPGYEWKAPKKAHNSHFSPDGGADEFDYSSWDAMCYLDPSKAGEEDDFVVGFWNPSEENCGTDIGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPPEKQSSGGSAELQYGWLKIPPRCMFEHPDAERTLNHLISGFLNFESKINYTFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVQNN

Target ORF information:

RefSeq Version	XM_005158666.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio dicer 1, ribonuclease type III (dicer1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158666.4

ORF Insert Sequence:

ATGGCTGGCC TACAGCTGGT GACCCCTGCC TCCTCACCCA TGGGGCCCTT CTTTGGTCTC 
CCGTGGCAAC AGGAAGCTAT CCATGACAAC ATATACACAC CAAGGAAATA TCAGGTTGAA 
CTTCTTGAAG CAGCTCTTGA ACATAATACT ATAGTCTGCT TAAATACTGG CTCAGGGAAG 
ACCTTTATCG CAGTGCTCCT AATCAAAGAA CTCTCCCACC AAATTCGTGG CGAGAATGGG 
AAGAGGACAG TTTTCCTGGT GAATGCAGCG TCATCTGTGG CTCAGCAAGC ATCTACTGTA 
AGAACACACT CTGATCTCCA AGTAGGCGAC TATATGTCAG AGGACATGAC ATCATGGCCC 
GAGGAGATGT GGAACAGAGA GATGATAGAG AATCAGGTGC TGGTGATGAC GTGCCACATC 
TTCCTGCATG TTTTAAAGAA TGGAGTCTTG CCACTATCAA AGATCAACCT GCTGGTTTTT 
GACGAATGCC ACCTTGCAAT CACTGGCCAT CCTTATCGGG AAATAATGAA GATTTGTGAA 
GGTTGCCCCA GCTGTCCTCG CATTTTGGGT CTCACTGCTT CCATTTTAAA TGGCAAATGT 
GACCCTTGTG ATTTGGAGGA GAAAATTCAG AACCTGGAGA AGATTCTTCA GAGCAATGCA 
GAAACTGCCA CTGATCTTGT GGTGCTGGAT AGGTATGCGT CGCAGCCGCG TGAGGAGGTG 
CTGGATTGTG GACAGTACCA AGATCAGAGT GGGCTCTCGG AGAGGCTTTT AAATGAGCTG 
GACGAAGCCC TCAATTTTCT AAACGATTGC AACTTGTCTG TACATAGAGA AGATAGAGAC 
CCCACGTTTA TCTCCAAACA GGTGCTGAAT GACTGTCGTG CTGTGTTGAC TGTGCTGGGC 
CCTTGGTGTG CGGATAAAGC TGCAGGAATC ATGGTTCGGG AGCTGCAGAA ATATATTAAA 
CATGAACAGG AAGAGCTAAA CCGCAAGTTC CTGTTGTTCA CCGACACCAT CCTGAGGAAA 
ATCCATGCCT TATGTGAGGA GCATTTTTCC CCAGCTTCCC TCGACCTGAA GTTTGTCACT 
CCCAAGGTCA TCAGACTTTT GGAGATTCTC CACGAGTACA AGCCTTTCGA ACGGCAGCAG 
TTTGAGAGTG TGGAGTGGTA CAACAATCGC AATCAGGACA ATTATGTTTC TTGGAGTGAT 
TCTGAAGATG ATGATGAGGA CGAAGAGGCG GAGGCAAAGG AGAAAACCGA AGCAAATTTT 
CCCTCGCCGT TCACAAATAT CCTCTGTGGA ATCATCTTTG TGGAAAGGCG ATATACAGCG 
GTTGTTTTAA ACAGACTTAT TAAGGAGGCA GGAAAGCAAG ACCCAGAGCT GGCTTACATC 
AGTAGCAACT TCATCACAGG GCACAGCATC GGCAAGAACC AGCCTCGCAA CAAGCAGATG 
GAGGTGGAGT TCAGAAAACA AGAGGAAGTC TTGAGGAAAT TCAGAGCCCA TGAAACCAAC 
TTGCTCATTG CAACCAGCAT TGTGGAGGAA GGCGTCGATA TCCCAAAGTG CAATTTGGTC 
GTTCGGTTTG ACCTGCCGAC AGAGTACCGG TCGTACGTGC AATCCAAAGG TCGAGCCCGT 
GCTCCAGTCT CCAACTACAT CATGCTAGCA GACAGCGAAC GGACTAAAAC CTTCCAAGAA 
GACCTCAAGA CCTACAAGGC CATAGAGAAG ATCCTCCGAA ATAAGTGCTC GAAGTCGGCT 
GAATGCAATG ATTTTGAGCT GGAGCCTGTG ACCGATGACG ACAATGTTCT CCCACCGTAC 
GTCCTGCGCT CAGAGGACGG CGGGCCTCGT GTCACCATGA ACACTGCCAT TGGGCACGTC 
AACCGATATT GTGCGCGCTT GCCCAGTGAT CCGTTCACTC ATCTCGCTCC GAAGTGTAAA 
ACTGTAGAGA TGAACACCGG AGGGTATCGA TCCACACTCT TCCTACCTAT CAACTCACCA 
CTCAGAGTTC CTGTCACCGG GCCAGTAATG AATTGTGCAA GACTGGCTGA GAAAGCTGTC 
GCTCTTCTCT GTTGTGAAAA ACTCCACAAA ATTGGTGAAC TGGATGATCA TCTGATGCCT 
GTGGGAAAGG AAACTGTGAA GTATGAGGAG GAGCTGGACC TGCATGATGA AGAGGAGACA 
AGCGTGCCAG GAAGACCAGG CTCCACTAAA CGGAGACAGT GCTCCCCAAA AGCCATACCA 
GAGTGTCTGC GGGGTTGTTA CCCTGTGCCA GAGCAGCCTT GCTACCTTTA CGTTATTGGG 
ATGGTCCTCA CCACCCCTCT ACCTGACGAA CTCAACTTCC GGCGGAGGAA GCTGTATCCA 
CCAGAGGACA CAACCCGATG TTTTGGCATT CTGACAGCTA AACCAATACC CCGGATCCCT 
CATTTCCCTG TGTACACTCG TTCTGGTGAG GTGACCATCT CCATTGAGCT GCAGAAGTCG 
GGCTTTAGTC TAAGTGCGGA GCAGCTGGAG CTGATCACAC GTCTGCATCA GTACATCTTC 
TCCCACATCC TCCGTCTGGA AAAGCCAGCA CTAGAGTTTA AGCCTGTAGA GGCAGATTCT 
GCCTACTGTG TTCTACCTCT CAACATCGTT GAGGATTCCA ATACTTTGGA TCTGGATTTC 
AAGTTCATGG AGGATATTGA AAAGTCTGAA GCACGCATTG GCATTCCAAA CACTCAGTAC 
ACCAAGCAAA ACCCATTCAT CTTCAAATTG GAGGACTACC AGGATGCTGT CATCATTCCA 
AGGTATCGTA ATTTTGACCA GCCGCATCGG TTCTACGTGG CTGACGTTTA CACAGACCTC 
ACCCCGCTCA GTAAATTCCC CTCTCCAGAG TACGAGACTT TTGCCGAATA CTATAAAACC 
AAGTACAACC TGGACCTGTC AAATGTTAAT CAGCCCCTAC TGGATGTGGA CCACACATCT 
TCAAGATTGA ATCTTTTAAC TCCCCGGCAC CTTAATCAAA AGGGGAAAGC CCTTCCTCTC 
AGCAGTGCGG AGAAGAGAAA GGCCAAGTGG GAAAGCCTAC AAAACAAACA GATTTTAGTT 
CCGGAGCTCT GTGCCATTCA CCCCATCCCA GCCTCTTTGT GGAGGAAAGC CGTCTGCCTT 
CCCAGCATTC TCTATAGACT TCACTGCCTT CTGACGGCCG AAGAGCTGCG GTCTCAGACA 
GCCATCGATG CTGGCGTAGG GGCTCAGACC TTGCCTCCTG ATTTCAGGTA TCCTAATTTG 
GATTTCGGAT GGAAGAAATC CATCGACAGC AAGTCTTTCA TCTCCTGCCC CAGTGCATGT 
ATGGAAGAAG ACGACGATCA CTGTAAACTC GGCACAAGCT CCGATTCAAA CCACACTGCC 
CCCGAGAGCT GCAGTATGGA GGTCTCCCAG CCTCCAGAAG GCGCTCCCAA CACACCAGAT 
GAAAAGCTAG AAACCCTCAC TCTCCCTGTG ACTGATCTAA ACAAAGACTG TTTCCCAAAC 
CTCCCAAACG GCACTCAGGC CGACAGCGAC GACCTCCCTC ACAGAAGTGA CGTTTGCCAA 
TGCTCTCAGC TGGGCCCTCT GGAGAGAGAC CTATCAACAC AAACCACTAC CTCAGTTTCA 
GTGCGGCCCT CTCCAGCTGG AGAACCACAG CCATGGCCCA GCGATGAATG TACAGGCAGG 
AGCTCGGATC TCTGCGACCC ACATGTGAAG AAACCTACCT CAAAGCATTG CCCTAAATCG 
GAGACGGCGA CTAGCACCCC AGCACCTTCA GAAACGTCAT CAGAGGACTG TAGATCAGCC 
TGCGCTGGTC CAGCCTGGGA TTCGCCCAAA ACCCTGGGCC CGAACCCCGG GCTCATCCTG 
CAGGCCCTGA CTCTCTCCAA CGCCAGTGAC GGCTTCAATC TGGAACGCCT GGAGATGCTG 
GGCGACTCCT TCCTGAAACA CGCCATCACC ACATACCTGT TCTGCACTTA CCCTGACGCC 
CACGAGGGCA GACTGTCCTA CATGAGAAGC AAGAAGGTCA GCAACTGTAA CCTGTATCGG 
CTGGGGAAGA AGAAGGGTCT TCCGAGTCGA ATGGTGGTGT CCATCTTTGA CCCGCCGGTA 
AACTGGCTTC CTCCAGGCTA CGTCGTGAAT CAGGACAAAA GCAGCACAGA CAAGTGGGAC 
TCAGATGAGA ATAAAGATTT AGCGAATGGA AAAGCCAGCG ATGACGAAGA CGAGGATGAC 
GATGATGAGC CGGAAGAAGC AGAGGTCGAG CCATCTAAAG AAGATGTAAA TGTTGAAGAC 
GACCTGGAGT ATTATTACGA ACACATCCGC TTCATCGACA GCATGTTAAT CGGCTCTGGC 
GCTTTCGGCA AGAAGATCTC CCTCCAGCCC ACAGACCCCG GCTACGAATG GAAAGCTCCT 
AAAAAAGCCC ACAACTCCCA CTTCTCGCCA GACGGGGGTG CTGACGAGTT CGATTACAGC 
TCCTGGGACG CCATGTGTTA CCTGGACCCC AGTAAAGCAG GTGAGGAGGA CGATTTCGTG 
GTGGGCTTCT GGAATCCCTC TGAGGAAAAC TGCGGCACGG ACATCGGGAA ACAGTCCATC 
TCTTATGACC TTCACACGGA GCAGTGCATC GCAGATAAGA GCATAGCGGA CTGTGTGGAG 
GCTCTGCTGG GCTGCTATCT GACCAGCTGT GGTGAGCGAG CGGCTCAGCT GTTTCTCTGC 
TCTCTGGGCC TGAAGGTGCT ACCACCGGAG AAGCAGAGTT CGGGAGGCTC TGCCGAGCTC 
CAGTACGGCT GGCTGAAGAT CCCACCACGC TGCATGTTCG AACATCCTGA CGCTGAACGC 
ACGCTCAACC ACCTCATCTC TGGCTTCCTG AACTTCGAGA GCAAGATCAA CTACACGTTT 
AAGAACAAGG CGTACCTGCT GCAGGCCTTC ACACACGCCT CGTATCATTA CAACACCATT 
ACAGATTGTT ATCAGCGGTT GGAGTTTCTT GGTGATGCAA TTCTAGACTA CCTCATAACC 
AAGCACCTTT ATGAAGACCC CCGGCAGCAC TCGCCCGGCG TGCTGACCGA CCTGCGCTCT 
GCACTTGTCA ACAACACCAT ATTCGCTTCC CTGGCTGTCA AGTATGATTA CCACAAGTAC 
TTCAAAGCTG TCTCGCCAGA ACTCTTCCAC GTGATCGACG ATTTTGTGCA ATTTCAACTG 
GAGAAGAATG AAATGCAAGG AATGGATTCA GAGCTTCGCC GATCGGAGGA AGATGAAGAG 
AAAGAGGAAG ACATTGAAGT CCCCAAAGCA ATGGGAGACA TCTTTGAATC GCTAGCTGGT 
GCAATTTACA TGGATAGCGG GATGTCACTT GAGACAGTTT GGCAAGTGTA TTATCCAATG 
ATGAGGCCGC TCATAGAAAA ATTCTCCGCT AATGTCCCTC GATCCCCAGT GCGTGAACTC 
CTGGAGATGG AGCCAGAGAC AGCTAAGTTC AGCCCTGCGG AAAGAACTTA TGATGGAAAG 
GTACGGGTGA CGGTGGAAGT CGTGGGCAAA GGCAAGTTCA AAGGCGTAGG ACGCAGCTAC 
CGGATAGCCA AGTCAGCAGC CGCCCGCCGA GCCCTGCGCA GTCTCAAAGC CAACCAACCT 
CAGGTCCAAA ACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Histological Characterization of the Dicer1 Mutant Zebrafish Retina.
Journal of ophthalmology2015309510(2015)
Akhtar S,Patnaik SR,Kotapati Raghupathy R,Al-Mubrad TM,Craft JA,Shu X

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dicer1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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