dlat cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	dlat
Entrez Gene ID	324201
Full Name	dihydrolipoamide S-acetyltransferase (E2 component of pyruvate dehydrogenase complex)
Synonyms	wu:fc14f10,wu:fc21f08,wu:fc86g11,wu:fj57d06
General protein information	Preferred Names dihydrolipoamide S-acetyltransferase (E2 component of pyruvate dehydrogenase complex) Names dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial fc86g11 fj57d06 no optokinetic nystagmus a no optokinetic response b noa nrb
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_212667.1	NP_997832.1	dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial
NM_212667.2	NP_997832.2	dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial

Pathways from BioSystems

Homologies

Homo sapiens (human)	DLAT	NP_001922.2
Rattus norvegicus (Norway rat)	Dlat	NP_112287.1
Drosophila melanogaster (fruit fly)	CG5261	NP_001260196.1
Magnaporthe oryzae (rice blast fungus)	MGG_09878	XP_003720354.1
Arabidopsis thaliana (thale cress)	AT3G13930	NP_566470.1
Xenopus tropicalis (tropical clawed frog)	dlat	NP_001016320.2
	DLAT	XP_001107013.1
Canis lupus familiaris (dog)	DLAT	XP_546524.2
Mus musculus (house mouse)	Dlat	NP_663589.3
Oryza sativa (rice)	Os07g0410100	NP_001059445.1
Bos taurus (cattle)	DLAT	NP_001192659.2
Gallus gallus (chicken)	DLAT	XP_417933.3
Neurospora crassa	NCU07659	XP_962786.1
Pan troglodytes (chimpanzee)	DLAT	XP_003313345.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007975	XP_317493.4
Caenorhabditis elegans (roundworm)	dlat-1	NP_506579.1
Arabidopsis thaliana (thale cress)	AT1G54220	NP_001031186.1
Danio rerio (zebrafish)	dlat	NP_997832.1
Saccharomyces cerevisiae (baker's yeast)	LAT1	NP_014328.3
Kluyveromyces lactis	KLLA0F04741g	XP_455294.1
Eremothecium gossypii	AGOS_AER364W	NP_985220.1
Schizosaccharomyces pombe (fission yeast)	lat1	NP_587755.1
Oryza sativa (rice)	Os06g0105400	NP_001056555.1
Oryza sativa (rice)	Os02g0105200	NP_001045618.1

Gene Ontology
Bibliography

Related articles in PubMed

Phenylbutyrate therapy for pyruvate dehydrogenase complex deficiency and lactic acidosis.
Ferriero R, Manco G, Lamantea E, Nusco E, Ferrante MI, Sordino P, Stacpoole PW, Lee B, Zeviani M, Brunetti-Pierri N
Science translational medicine5(175)175ra31(2013 Mar)

The 13q and 11q B-cell chronic lymphocytic leukaemia-associated regions derive from a common ancestral region in the zebrafish.
Auer RL, Riaz S, Cotter FE
British journal of haematology137(5)443-53(2007 Jun)

Temperature- and exercise-induced gene expression and metabolic enzyme changes in skeletal muscle of adult zebrafish (Danio rerio).
McClelland GB, Craig PM, Dhekney K, Dipardo S
The Journal of physiology577(Pt 2)739-51(2006 Dec)

A zebrafish model for pyruvate dehydrogenase deficiency: rescue of neurological dysfunction and embryonic lethality using a ketogenic diet.
Taylor MR, Hurley JB, Van Epps HA, Brockerhoff SE
Proceedings of the National Academy of Sciences of the United States of America101(13)4584-9(2004 Mar)

Behavioral genetic approaches to visual system development and function in zebrafish.
Neuhauss SC
Journal of neurobiology54(1)148-60(2003 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Deficiency of dihydrolipoamide S-acetyltransferase (Dlat) results in neurological dysfunction, congenital lactic acidosis, growth retardation, and early death.
Title: A zebrafish model for pyruvate dehydrogenase deficiency: rescue of neurological dysfunction and embryonic lethality using a ketogenic diet.

The following dlat gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dlat cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa36280	NM_212667.1 Latest version!	Danio rerio dihydrolipoamide S-acetyltransferase (E2 component of pyruvate dehydrogenase complex) (dlat), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa159694	NM_212667.2 Latest version!	Danio rerio dihydrolipoamide S-acetyltransferase (E2 component of pyruvate dehydrogenase complex) (dlat), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dlat ( NM_212667.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_997832.1 Danio rerio dihydrolipoamide S-acetyltransferase (E2 component of pyruvate dehydrogenase complex) (dlat), mRNA.

Price & Availability↑

CloneID	ODa36280
Clone ID Related Accession (Same CDS sequence)	NM_212667.1
Accession Version	NM_212667.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-07-02
Organism	Danio rerio(zebrafish)
Product	dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY188775.1. ##Evidence-Data-START## Transcript exon combination :: AY188775.1, BC163264.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476745, SAMEA4476770 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGTTGCGTT TAGTGCAGCG AGTGCGCCCA TCTGCCAGGC TCACCCGGCC CAGTGTCCTC 
TCGGGCCCTG CTGCTGCTCC TGCTGCCAGT CCTCGACCCG GACCAGTGTC CTGCCATCAT 
CTCAAACGGT ACCACTCCAG CGGGCCTGCC CGGATCCGGA GCTCGTTTCA CGGTCGGCGA 
GTCTGCAACA GCATCCTCAG TAACAGATCA TGGGGGCTGA GAAGTCAAAC GGCCGCTTTC 
AGTCAGAGCA TGAGAGTCTA CAGCCTGCCG CCGCATCAGA AGGTGGAACT ACCTGCCTTG 
TCCCCCACAA TGCAGATGGG CACTATTGCA CGCTGGGAGA AGAAGGAAGG TGACAAAATC 
AATGAGGGAG ATTTGATTGC TGAGGTTGAA ACAGACAAGG CTACAGTTGG ATTTGAAATG 
TTGGAAGAGT GCTATCTAGC CAAGATCCTG GTTGCAGAGG GTACAAGAGA TGTGCCCATT 
GGAGCAGTCA TTTGTATTAC TGTTGACAAA CCTGAGCTCA TCTCTTCATT CAAGGACTTT 
ACATTGGATA AAATCACCAG CTCTGCCCCT GCTGCTGCAG CTCCTCCCCC TCCAGCCACA 
CCCACTTCTG CTCCTGCAGC CCCACAAGTA CCTGGAAGCT CCTACCCGCC TCATATGAAG 
GTGCTTCTTC CAGCTCTCTC ACCCACCATG ACTATGGGCA CCGTGCAGCG CTGGGAGAAG 
AAAGTAGGTG AGAAGCTGAG TGAGGGAGAC CTGCTGGCTG AAATCGAGAC AGATAAGGCA 
ACAATCGGTT TTGAGGTTCA GGAGGAAGGC TACCTGGCTA AGATCATGAT TTCTGAGGGC 
ACCAGAGACG TTCCTCTCGG TACTCCGCTC TGTATCATCG TGGAAAAGGA ATCAGACATT 
AGTGCATTTG CAGACTATGT AGAGACAGGG GTTGCTGCTA GCCCACCTCC CGCTCCCACA 
CTGGTGGCCA CTCCACCACC AGCAGCAGCT CCAGCAGCTC CCATCCCGGC CCCTGCTGCT 
GCTCCTGCCG CTCCTGCTGC TGCTAGGAAA GGAAGGGTGT TTGCCAGTCC TTTAGCCAAG 
AAGCTGGCAG CTGAGAAGGG AGTTGACATC ACTCAAGTCA CTGGAACTGG ACCAGATGGC 
AGAGTCACCA AGAAAGATAT TGACAGTTTT GTTCCACCAA AGCTCACTCC TGCTGCAGCT 
GCTGCCCCGT CTGCACCCAC CCCCAGTCCT CCTGCTGCCC CTGCTTATGC TGCTGTACCT 
ACTGGAACCT TCACGGACGT CCCTATCAGC AATATCCGCA AAGTGATTGC CCAGAGGTTG 
ATGCAGTCAA AACAGACTAT TCCTCACTAT TATCTGTCTA TCGATGTCAA CATGGACCAA 
GTTCTTGAAC TCAGGAAAGA GCTCAATGCT GAGGTGAAAG CTGAAAACAT CAAACTCTCG 
GTCAATGACT TCATCATCAA GGCCTCGGCT CTAGCCTGTT TAAAGGTGCC GGAGGCCAAT 
TCCTCTTGGA TGGACACAGT CATAAGACAA AATCACGTGG TGGATGTTAG TGTGGCGGTC 
AGCACGCCTG TTGGTCTCAT CACTCCCATT GTATTTAATG CTCATATTAA GGGCCTTGCA 
AATATCAGCA AAGACGTCTC CGCGTTAGCT GCTAAAGCAC GAGATGGGAA ACTACAACCT 
CATGAATTCC AGGGTGGAAC CTTCACTATT TCCAATCTGG GCATGTATGG CATCAAGCAT 
TTTTCAGCTA TCATTAATCC TCCTCAAGCC TGCATTCTGG CTGTGGGTGG CTCAGAAAAG 
AGACTGCTGC CTGCGGACAA TGAAAAAGGG TTTGACGTGG CTAACATGAT GTCTGTGACT 
CTGAGCTGCG ATCACCGGGT GGTTGATGGT GCTGTTGGCG CTCAATGGCT GGCAGAGTTT 
CGCAAGTTCC TAGAGAAACC ATTTACCATG CTTCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997832.1
CDS	1..1959
Translation	MLRLVQRVRPSARLTRPSVLSGPAAAPAASPRPGPVSCHHLKRYHSSGPARIRSSFHGRRVCNSILSNRSWGLRSQTAAFSQSMRVYSLPPHQKVELPALSPTMQMGTIARWEKKEGDKINEGDLIAEVETDKATVGFEMLEECYLAKILVAEGTRDVPIGAVICITVDKPELISSFKDFTLDKITSSAPAAAAPPPPATPTSAPAAPQVPGSSYPPHMKVLLPALSPTMTMGTVQRWEKKVGEKLSEGDLLAEIETDKATIGFEVQEEGYLAKIMISEGTRDVPLGTPLCIIVEKESDISAFADYVETGVAASPPPAPTLVATPPPAAAPAAPIPAPAAAPAAPAAARKGRVFASPLAKKLAAEKGVDITQVTGTGPDGRVTKKDIDSFVPPKLTPAAAAAPSAPTPSPPAAPAYAAVPTGTFTDVPISNIRKVIAQRLMQSKQTIPHYYLSIDVNMDQVLELRKELNAEVKAENIKLSVNDFIIKASALACLKVPEANSSWMDTVIRQNHVVDVSVAVSTPVGLITPIVFNAHIKGLANISKDVSALAAKARDGKLQPHEFQGGTFTISNLGMYGIKHFSAIINPPQACILAVGGSEKRLLPADNEKGFDVANMMSVTLSCDHRVVDGAVGAQWLAEFRKFLEKPFTMLL

Target ORF information:

RefSeq Version	NM_212667.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio dihydrolipoamide S-acetyltransferase (E2 component of pyruvate dehydrogenase complex) (dlat), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_212667.1

ORF Insert Sequence:

ATGTTGCGTT TAGTGCAGCG AGTGCGCCCA TCTGCCAGGC TCACCCGGCC CAGTGTCCTC 
TCGGGCCCTG CTGCTGCTCC TGCTGCCAGT CCTCGACCCG GACCAGTGTC CTGCCATCAT 
CTCAAACGGT ACCACTCCAG CGGGCCTGCC CGGATCCGGA GCTCGTTTCA CGGTCGGCGA 
GTCTGCAACA GCATCCTCAG TAACAGATCA TGGGGGCTGA GAAGTCAAAC GGCCGCTTTC 
AGTCAGAGCA TGAGAGTCTA CAGCCTGCCG CCGCATCAGA AGGTGGAACT ACCTGCCTTG 
TCCCCCACAA TGCAGATGGG CACTATTGCA CGCTGGGAGA AGAAGGAAGG TGACAAAATC 
AATGAGGGAG ATTTGATTGC TGAGGTTGAA ACAGACAAGG CTACAGTTGG ATTTGAAATG 
TTGGAAGAGT GCTATCTAGC CAAGATCCTG GTTGCAGAGG GTACAAGAGA TGTGCCCATT 
GGAGCAGTCA TTTGTATTAC TGTTGACAAA CCTGAGCTCA TCTCTTCATT CAAGGACTTT 
ACATTGGATA AAATCACCAG CTCTGCCCCT GCTGCTGCAG CTCCTCCCCC TCCAGCCACA 
CCCACTTCTG CTCCTGCAGC CCCACAAGTA CCTGGAAGCT CCTACCCGCC TCATATGAAG 
GTGCTTCTTC CAGCTCTCTC ACCCACCATG ACTATGGGCA CCGTGCAGCG CTGGGAGAAG 
AAAGTAGGTG AGAAGCTGAG TGAGGGAGAC CTGCTGGCTG AAATCGAGAC AGATAAGGCA 
ACAATCGGTT TTGAGGTTCA GGAGGAAGGC TACCTGGCTA AGATCATGAT TTCTGAGGGC 
ACCAGAGACG TTCCTCTCGG TACTCCGCTC TGTATCATCG TGGAAAAGGA ATCAGACATT 
AGTGCATTTG CAGACTATGT AGAGACAGGG GTTGCTGCTA GCCCACCTCC CGCTCCCACA 
CTGGTGGCCA CTCCACCACC AGCAGCAGCT CCAGCAGCTC CCATCCCGGC CCCTGCTGCT 
GCTCCTGCCG CTCCTGCTGC TGCTAGGAAA GGAAGGGTGT TTGCCAGTCC TTTAGCCAAG 
AAGCTGGCAG CTGAGAAGGG AGTTGACATC ACTCAAGTCA CTGGAACTGG ACCAGATGGC 
AGAGTCACCA AGAAAGATAT TGACAGTTTT GTTCCACCAA AGCTCACTCC TGCTGCAGCT 
GCTGCCCCGT CTGCACCCAC CCCCAGTCCT CCTGCTGCCC CTGCTTATGC TGCTGTACCT 
ACTGGAACCT TCACGGACGT CCCTATCAGC AATATCCGCA AAGTGATTGC CCAGAGGTTG 
ATGCAGTCAA AACAGACTAT TCCTCACTAT TATCTGTCTA TCGATGTCAA CATGGACCAA 
GTTCTTGAAC TCAGGAAAGA GCTCAATGCT GAGGTGAAAG CTGAAAACAT CAAACTCTCG 
GTCAATGACT TCATCATCAA GGCCTCGGCT CTAGCCTGTT TAAAGGTGCC GGAGGCCAAT 
TCCTCTTGGA TGGACACAGT CATAAGACAA AATCACGTGG TGGATGTTAG TGTGGCGGTC 
AGCACGCCTG TTGGTCTCAT CACTCCCATT GTATTTAATG CTCATATTAA GGGCCTTGCA 
AATATCAGCA AAGACGTCTC CGCGTTAGCT GCTAAAGCAC GAGATGGGAA ACTACAACCT 
CATGAATTCC AGGGTGGAAC CTTCACTATT TCCAATCTGG GCATGTATGG CATCAAGCAT 
TTTTCAGCTA TCATTAATCC TCCTCAAGCC TGCATTCTGG CTGTGGGTGG CTCAGAAAAG 
AGACTGCTGC CTGCGGACAA TGAAAAAGGG TTTGACGTGG CTAACATGAT GTCTGTGACT 
CTGAGCTGCG ATCACCGGGT GGTTGATGGT GCTGTTGGCG CTCAATGGCT GGCAGAGTTT 
CGCAAGTTCC TAGAGAAACC ATTTACCATG CTTCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dlat ( NM_212667.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_997832.2 Danio rerio dihydrolipoamide S-acetyltransferase (E2 component of pyruvate dehydrogenase complex) (dlat), mRNA.

Price & Availability↑

CloneID	ODa159694
Clone ID Related Accession (Same CDS sequence)	NM_212667.2
Accession Version	NM_212667.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-20
Organism	Danio rerio(zebrafish)
Product	dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX296544.9 and CU694531.8. On Aug 4, 2018 this sequence version replaced NM_212667.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GDQH01006449.1, GDQQ01017196.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476745, SAMEA4476770 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGTTGCGTT TAGTGCTGCG AGTGCGCCCA TCTGCCAGGC TCACCCGGCC CAGTGTCCTC 
TCGGGCCCTG CTGCTGCTCC TGCTGCCAGT CCTCGACCCG GACCAGTGTC CTGCCATCAT 
CTCAAACGGT ACCACTCCAG CGGGCCTGCC CGGATCCGGA GCTCGTTTCA CGGTCGGCGA 
GTCTGCAACA GCATCCTCAG TAACAGATCA TGGGGGCTGA GAAGCCAAAC GGCCGCTTTC 
AGTCAGAGCA TGAGAGTCTA CAGCCTGCCG CCGCATCAGA AGGTGGAACT ACCTGCCTTG 
TCCCCCACAA TGCAGATGGG CACTATTGCA CGCTGGGAGA AGAAGGAAGG TGACAAAATC 
AATGAGGGAG ATTTGATTGC TGAGGTTGAA ACAGACAAGG CTACAGTTGG ATTTGAAATG 
TTGGAAGAGT GCTATCTAGC CAAGATCCTG GTTGCAGAGG GTACAAGAGA TGTGCCCATT 
GGAGCAGTCA TTTGTATTAC TGTTGACAAA CCTGAGCTCA TCTCTTCATT CAAGGACTTT 
ACATTGGATA AAATCACCAG CTCTGCCCCT GCTGCTGCAG CTCCTCCCCC TCCAGCCACA 
CCCACATCTG CTCCTGCAGC CCCACAAGTA CCTGGAAGCT CCTACCCGCC TCATATGAAG 
GTGCTTCTTC CAGCTCTCTC ACCCACCATG ACTATGGGCA CCGTGCAGCG CTGGGAGAAG 
AAAGTAGGTG AGAAGTTGAG TGAGGGAGAC CTGCTGGCTG AAATCGAGAC AGATAAGGCA 
ACAATCGGTT TTGAGGTTCA GGAGGAAGGC TACCTGGCTA AGATCATGAT TTCTGAGGGC 
ACCAGAGACG TTCCTCTCGG CACTCCGCTC TGTATCATCG TGGAAAAGGA ATCGGACATT 
AGTGCATTTG CAGACTATGT AGAGACAGGG GTTGCTGCTA GCCCACCTCC TGCTCCCACA 
CCGGTGGCCA CTCCACCACC AGCAGCAGCT CCAGCAGCTC CCATCCCGGC CCCTGCTGCT 
GCTCCTGCCG CTCCTGCTGC TGCTAGGAAG GGAAGGGTGT TTGCCAGTCC TTTAGCCAAG 
AAGCTGGCGG CTGAGAAGGG AGTTGACATC ACTCAAGTCA CTGGAACTGG ACCAGATGGC 
AGAGTCACCA AGAAAGATAT TGACAGTTTT GTTCCACCAA AGCTTGCTCC TGCTGCAGCT 
GCTGCCCCGT CTGCACCCAC CCCCAGTCCT CCTGCTGCCC CTGCTTATGC TGCTGTACCT 
ACAGGAACCT TCACGGACGT CCCTATCAGC AATATCCGCA AAGTGATCGC CCAGAGGTTG 
ATGCAGTCAA AACAGACTAT TCCACACTAT TATCTGTCTA TCGATGTCAA CATGGACCAA 
GTTCTTGAAC TCAGGAAAGA GCTCAATGCT GAGGTGAAAG CTGAAAACAT CAAACTCTCG 
GTCAATGACT TTATCATCAA GGCCTCGGCT CTAGCCTGTT TAAAGGTGCC GGAGGCCAAT 
TCCTCTTGGA TGGACACAGT CATAAGACAA AATCACGTGG TGGATGTTAG TGTGGCGGTC 
AGCACGCCTG TGGGTCTCAT CACTCCCATT GTATTTAATG CTCATATTAA GGGCCTTGCA 
AATATCAGCA AAGACGTCTC CGCGTTAGCT GCTAAAGCAC GAGATGGGAA ACTACAACCT 
CATGAATTCC AGGGTGGAAC CTTCACTATT TCCAATCTGG GCATGTATGG CATCAAGCAT 
TTTTCAGCTA TCATTAATCC TCCTCAAGCC TGCATTCTGG CTGTGGGTGG CTCAGAAAAG 
AGACTGCTGC CTGCGGACAA TGAAAAAGGG TTTGACGTGG CTAACATGAT GTCTGTGACT 
CTGAGCTGCG ATCACCGGGT GGTTGATGGT GCTGTTGGCG CTCAATGGCT GGCAGAGTTT 
CGCAAGTTCC TAGAGAAACC GTTTACCATG CTTCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997832.2
CDS	180..2138
Translation	MLRLVLRVRPSARLTRPSVLSGPAAAPAASPRPGPVSCHHLKRYHSSGPARIRSSFHGRRVCNSILSNRSWGLRSQTAAFSQSMRVYSLPPHQKVELPALSPTMQMGTIARWEKKEGDKINEGDLIAEVETDKATVGFEMLEECYLAKILVAEGTRDVPIGAVICITVDKPELISSFKDFTLDKITSSAPAAAAPPPPATPTSAPAAPQVPGSSYPPHMKVLLPALSPTMTMGTVQRWEKKVGEKLSEGDLLAEIETDKATIGFEVQEEGYLAKIMISEGTRDVPLGTPLCIIVEKESDISAFADYVETGVAASPPPAPTPVATPPPAAAPAAPIPAPAAAPAAPAAARKGRVFASPLAKKLAAEKGVDITQVTGTGPDGRVTKKDIDSFVPPKLAPAAAAAPSAPTPSPPAAPAYAAVPTGTFTDVPISNIRKVIAQRLMQSKQTIPHYYLSIDVNMDQVLELRKELNAEVKAENIKLSVNDFIIKASALACLKVPEANSSWMDTVIRQNHVVDVSVAVSTPVGLITPIVFNAHIKGLANISKDVSALAAKARDGKLQPHEFQGGTFTISNLGMYGIKHFSAIINPPQACILAVGGSEKRLLPADNEKGFDVANMMSVTLSCDHRVVDGAVGAQWLAEFRKFLEKPFTMLL

Target ORF information:

RefSeq Version	NM_212667.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio dihydrolipoamide S-acetyltransferase (E2 component of pyruvate dehydrogenase complex) (dlat), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_212667.2

ORF Insert Sequence:

ATGTTGCGTT TAGTGCTGCG AGTGCGCCCA TCTGCCAGGC TCACCCGGCC CAGTGTCCTC 
TCGGGCCCTG CTGCTGCTCC TGCTGCCAGT CCTCGACCCG GACCAGTGTC CTGCCATCAT 
CTCAAACGGT ACCACTCCAG CGGGCCTGCC CGGATCCGGA GCTCGTTTCA CGGTCGGCGA 
GTCTGCAACA GCATCCTCAG TAACAGATCA TGGGGGCTGA GAAGCCAAAC GGCCGCTTTC 
AGTCAGAGCA TGAGAGTCTA CAGCCTGCCG CCGCATCAGA AGGTGGAACT ACCTGCCTTG 
TCCCCCACAA TGCAGATGGG CACTATTGCA CGCTGGGAGA AGAAGGAAGG TGACAAAATC 
AATGAGGGAG ATTTGATTGC TGAGGTTGAA ACAGACAAGG CTACAGTTGG ATTTGAAATG 
TTGGAAGAGT GCTATCTAGC CAAGATCCTG GTTGCAGAGG GTACAAGAGA TGTGCCCATT 
GGAGCAGTCA TTTGTATTAC TGTTGACAAA CCTGAGCTCA TCTCTTCATT CAAGGACTTT 
ACATTGGATA AAATCACCAG CTCTGCCCCT GCTGCTGCAG CTCCTCCCCC TCCAGCCACA 
CCCACATCTG CTCCTGCAGC CCCACAAGTA CCTGGAAGCT CCTACCCGCC TCATATGAAG 
GTGCTTCTTC CAGCTCTCTC ACCCACCATG ACTATGGGCA CCGTGCAGCG CTGGGAGAAG 
AAAGTAGGTG AGAAGTTGAG TGAGGGAGAC CTGCTGGCTG AAATCGAGAC AGATAAGGCA 
ACAATCGGTT TTGAGGTTCA GGAGGAAGGC TACCTGGCTA AGATCATGAT TTCTGAGGGC 
ACCAGAGACG TTCCTCTCGG CACTCCGCTC TGTATCATCG TGGAAAAGGA ATCGGACATT 
AGTGCATTTG CAGACTATGT AGAGACAGGG GTTGCTGCTA GCCCACCTCC TGCTCCCACA 
CCGGTGGCCA CTCCACCACC AGCAGCAGCT CCAGCAGCTC CCATCCCGGC CCCTGCTGCT 
GCTCCTGCCG CTCCTGCTGC TGCTAGGAAG GGAAGGGTGT TTGCCAGTCC TTTAGCCAAG 
AAGCTGGCGG CTGAGAAGGG AGTTGACATC ACTCAAGTCA CTGGAACTGG ACCAGATGGC 
AGAGTCACCA AGAAAGATAT TGACAGTTTT GTTCCACCAA AGCTTGCTCC TGCTGCAGCT 
GCTGCCCCGT CTGCACCCAC CCCCAGTCCT CCTGCTGCCC CTGCTTATGC TGCTGTACCT 
ACAGGAACCT TCACGGACGT CCCTATCAGC AATATCCGCA AAGTGATCGC CCAGAGGTTG 
ATGCAGTCAA AACAGACTAT TCCACACTAT TATCTGTCTA TCGATGTCAA CATGGACCAA 
GTTCTTGAAC TCAGGAAAGA GCTCAATGCT GAGGTGAAAG CTGAAAACAT CAAACTCTCG 
GTCAATGACT TTATCATCAA GGCCTCGGCT CTAGCCTGTT TAAAGGTGCC GGAGGCCAAT 
TCCTCTTGGA TGGACACAGT CATAAGACAA AATCACGTGG TGGATGTTAG TGTGGCGGTC 
AGCACGCCTG TGGGTCTCAT CACTCCCATT GTATTTAATG CTCATATTAA GGGCCTTGCA 
AATATCAGCA AAGACGTCTC CGCGTTAGCT GCTAAAGCAC GAGATGGGAA ACTACAACCT 
CATGAATTCC AGGGTGGAAC CTTCACTATT TCCAATCTGG GCATGTATGG CATCAAGCAT 
TTTTCAGCTA TCATTAATCC TCCTCAAGCC TGCATTCTGG CTGTGGGTGG CTCAGAAAAG 
AGACTGCTGC CTGCGGACAA TGAAAAAGGG TTTGACGTGG CTAACATGAT GTCTGTGACT 
CTGAGCTGCG ATCACCGGGT GGTTGATGGT GCTGTTGGCG CTCAATGGCT GGCAGAGTTT 
CGCAAGTTCC TAGAGAAACC GTTTACCATG CTTCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Phenylbutyrate therapy for pyruvate dehydrogenase complex deficiency and lactic acidosis.
Science translational medicine5(175)175ra31(2013 Mar)
Ferriero R,Manco G,Lamantea E,Nusco E,Ferrante MI,Sordino P,Stacpoole PW,Lee B,Zeviani M,Brunetti-Pierri N

The 13q and 11q B-cell chronic lymphocytic leukaemia-associated regions derive from a common ancestral region in the zebrafish.
British journal of haematology137(5)443-53(2007 Jun)
Auer RL,Riaz S,Cotter FE

Temperature- and exercise-induced gene expression and metabolic enzyme changes in skeletal muscle of adult zebrafish (Danio rerio).
The Journal of physiology577(Pt 2)739-51(2006 Dec)
McClelland GB,Craig PM,Dhekney K,Dipardo S

A zebrafish model for pyruvate dehydrogenase deficiency: rescue of neurological dysfunction and embryonic lethality using a ketogenic diet.
Proceedings of the National Academy of Sciences of the United States of America101(13)4584-9(2004 Mar)
Taylor MR,Hurley JB,Van Epps HA,Brockerhoff SE

Behavioral genetic approaches to visual system development and function in zebrafish.
Journal of neurobiology54(1)148-60(2003 Jan)
Neuhauss SC