scfd1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	scfd1
Entrez Gene ID	317732
Full Name	sec1 family domain containing 1
Synonyms	SI:dZ115O7.1,SI:dZ234G15.3,SI:dZ234G15.4,SI:dZ234G15.5,SI:dZ234G15.7,dZ234G15.2,si:busm1-234g15.2,si:dz234g15.2,sly1
General protein information	Preferred Names sec1 family domain containing 1 Names sec1 family domain-containing protein 1 emm emmental suppressor of ypt1 vesicle-transport
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_182861.1	NP_878281.1	sec1 family domain-containing protein 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SCFD1	XP_003952508.1
Caenorhabditis elegans (roundworm)	F43D9.3	NP_001122697.2
Homo sapiens (human)	SCFD1	NP_057190.2
	SCFD1	XP_001107671.2
Canis lupus familiaris (dog)	SCFD1	XP_537403.4
Drosophila melanogaster (fruit fly)	Slh	NP_995614.1
Kluyveromyces lactis	KLLA0D18480g	XP_453879.1
Magnaporthe oryzae (rice blast fungus)	MGG_09552	XP_003712189.1
Bos taurus (cattle)	SCFD1	NP_001178294.1
Danio rerio (zebrafish)	sly1	NP_878281.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011358	XP_309291.4
Eremothecium gossypii	AGOS_AGL120W	NP_986547.1
Arabidopsis thaliana (thale cress)	ATSLY1	NP_179389.1
Oryza sativa (rice)	Os03g0620800	NP_001050677.1
Mus musculus (house mouse)	Scfd1	NP_084101.1
Schizosaccharomyces pombe (fission yeast)	sly1	NP_588374.1
Rattus norvegicus (Norway rat)	Scfd1	NP_062237.1
Gallus gallus (chicken)	SCFD1	NP_001186643.1
Saccharomyces cerevisiae (baker's yeast)	SLY1	NP_010475.1
Neurospora crassa	NCU04252	XP_961243.2
Xenopus tropicalis (tropical clawed frog)	scfd1	NP_001135508.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

The Sec domain protein Scfd1 facilitates trafficking of ECM components during chondrogenesis.
Hou N, Yang Y, Scott IC, Lou X
Developmental biology421(1)8-15(2017 Jan)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

The BH3-only SNARE BNip1 mediates photoreceptor apoptosis in response to vesicular fusion defects.
Nishiwaki Y, Yoshizawa A, Kojima Y, Oguri E, Nakamura S, Suzuki S, Yuasa-Kawada J, Kinoshita-Kawada M, Mochizuki T, Masai I
Developmental cell25(4)374-87(2013 May)

Simplet controls cell proliferation and gene transcription during zebrafish caudal fin regeneration.
Kizil C, Otto GW, Geisler R, N?sslein-Volhard C, Antos CL
Developmental biology325(2)329-40(2009 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the existence of a specific export pathway, mediated by a complex containing SCFD1 and STX18 that plays an essential role in secretion of large extracellular matrix proteins during chondrogenesis.
Title: The Sec domain protein Scfd1 facilitates trafficking of ECM components during chondrogenesis.

sly1 is essential for blastemal organization and proliferation during two stages of fin regeneration
Title: Positional cloning of a temperature-sensitive mutant emmental reveals a role for sly1 during cell proliferation in zebrafish fin regeneration.

The following scfd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the scfd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa02168	NM_182861.1 Latest version!	Danio rerio sec1 family domain containing 1 (scfd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

scfd1 ( NM_182861.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_878281.1 Danio rerio sec1 family domain containing 1 (scfd1), mRNA.

Price & Availability↑

CloneID	ODa02168
Clone ID Related Accession (Same CDS sequence)	NM_182861.1
Accession Version	NM_182861.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1899bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	sec1 family domain-containing protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY167023.1. ##Evidence-Data-START## Transcript exon combination :: AY167023.1, BC050950.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGCGT CCATCCGGGA AAAACAGACA GCTGCTCTCA AACGAATGCT CAACTTTAAT 
GCCCCACCCC TTAAAAACAC AACAGCTGAG CCAGTATGGA AGGTTCTTAT TTACGATCGC 
TTTGGCCAAG ACATTATATC TCCACTTCTG TCTGTGAAAG AGCTACGAGA CATGGGCATC 
ACTCTGCACC TCTTGTTGCA CTCAGATCGC GACCCCATTC CAGACGTTCC TGCTATTTAC 
TTTATAATGC CCACAGAGGA GAACATCGAC AGGATATGTC AAGACCTCCG AAACCAACTA 
TATGAGTCCT ACTATTTGAA CTTCATCTCC GCAATTTCCA GAAGCAAATT GGAGGATATA 
GCAAGTGCTG CGCTTGCGGC TAATGCAGTA AACCAGGTGA CCAAAGTATT TGATCAGTAT 
TTGAACTTCA TCACACTTGA GGATGATATG TTCATTCTCT GTAATCAAAA CAAAGAGCAC 
ATCTCCTACC ATGCAATCAA TAAGCCAGAT ATCATGGACA CAGACATGGA GGGTATAATG 
GACACAATAG TGGATAGTCT CTTCTGCTTC TTTGTTACTC TTGGTGCTGT CCCAATAATT 
CGATGTCCAA GAGGGAATGC TGCCGAAATG GTAGCAGTGA AACTTGACAA GAAACTGAGA 
GAGAATTTGA GAGACGCTCG TAACAGCCTG TTCACCGGAG ACACCATGGG CACTGGCCAG 
TTCAGTTTCC AGAGGCCGTT GTTTGTTCTT GCGGATCGTA ACCTGGATCT TGCGACTCCT 
CTCCACCACA CATGGACGTA TCAGGCTCTA ATTCATGATG TGCTGGACTT CCACCTGAAC 
CGAGTGAGTG TGGACGAGTC TCATGGGTCA GAGGCCAGTC CAGCCGGAGC ACGACCCAAA 
AAGAAAAACA AGAAGAGCTA TGATCTCACT GCTGCTGACA AGTTTTGGCA AAAACACAAA 
GGAAGCCCAT TCCCAGAGGT TGCAGAAGCT GTGCAGGAGG AACTGGACAC ATATCGAGCC 
CAAGAGGATG AGGTTAAACG GCTCAAGAGC ATCATGGGCT TAGAAGGGGA GGACGACGGA 
GCCATTAGTA TGTTGTCAGA TAACACTGCC AAACTTACCT CAGCCGTCAG TTCTCTTCCT 
GAGCTCCTGG AAAAGAAGAG ACTGATTGAC CTCCACACCA ACGTTGCCAC AGCAGTTCTG 
GACCACATCA AGAGCCGTAA GCTTGACGTG TACTTCGAGT ACGAGGAGAA GCTCATGAGC 
AAATCCACAC TCGATAAATC CCTGCTGGAC ATTATCAGTG ACCCTGATGC TGGAACGGCA 
GAGGATAAGA TGAGGCTGTT TCTGATCTAC TACATCACTT CCCAGCAGCC CCCATCGGAG 
GCTGATCTAG AACAGTACAA GAAAGCCATG GTTGATGCAG GCTGCGACCT ATCCCCACTT 
AACTACATTA AACAGTGGAA AGCTTTCACC AAGATGGCTG CGACGCCAGC CAATTACGGG 
AGCAGTGGAG TGAAACCTAT GGGACTGTTC TCAAGGGTGA TGAACACCGG CTCCCAGTTT 
GTGATGGAGG GAGTGAAAAA TCTGGTCTTG AAACAGCATA ACCTACCGGT TACTCGCATC 
TTGGACAACT TGATGGAAAT GAAGTCAAAC CCAGAGACTG ATGACTACAG ATACTTTGAT 
CCCAAGATGC TTCGTGGAAG CGAGAGCTCC ATTCCGAGGA ACAAGAACCC CTTTCAGGAG 
GCTATCGTCT TTGTTGTTGG AGGAGGCAAT TACATAGAGT ATCAAAATCT TGTTGACTAC 
ACAAAGACCA GGCAAGGAAA GCGGATTCTT TATGGCTGTA GTGAGCTGTT CAATGCTGCC 
CAGTTCATTA AACAGCTCTC TCAGCTTGGT CAGAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_878281.1
CDS	55..1953
Translation	MAASIREKQTAALKRMLNFNAPPLKNTTAEPVWKVLIYDRFGQDIISPLLSVKELRDMGITLHLLLHSDRDPIPDVPAIYFIMPTEENIDRICQDLRNQLYESYYLNFISAISRSKLEDIASAALAANAVNQVTKVFDQYLNFITLEDDMFILCNQNKEHISYHAINKPDIMDTDMEGIMDTIVDSLFCFFVTLGAVPIIRCPRGNAAEMVAVKLDKKLRENLRDARNSLFTGDTMGTGQFSFQRPLFVLADRNLDLATPLHHTWTYQALIHDVLDFHLNRVSVDESHGSEASPAGARPKKKNKKSYDLTAADKFWQKHKGSPFPEVAEAVQEELDTYRAQEDEVKRLKSIMGLEGEDDGAISMLSDNTAKLTSAVSSLPELLEKKRLIDLHTNVATAVLDHIKSRKLDVYFEYEEKLMSKSTLDKSLLDIISDPDAGTAEDKMRLFLIYYITSQQPPSEADLEQYKKAMVDAGCDLSPLNYIKQWKAFTKMAATPANYGSSGVKPMGLFSRVMNTGSQFVMEGVKNLVLKQHNLPVTRILDNLMEMKSNPETDDYRYFDPKMLRGSESSIPRNKNPFQEAIVFVVGGGNYIEYQNLVDYTKTRQGKRILYGCSELFNAAQFIKQLSQLGQK

Target ORF information:

RefSeq Version	NM_182861.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sec1 family domain containing 1 (scfd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_182861.1

ORF Insert Sequence:

ATGGCGGCGT CCATCCGGGA AAAACAGACA GCTGCTCTCA AACGAATGCT CAACTTTAAT 
GCCCCACCCC TTAAAAACAC AACAGCTGAG CCAGTATGGA AGGTTCTTAT TTACGATCGC 
TTTGGCCAAG ACATTATATC TCCACTTCTG TCTGTGAAAG AGCTACGAGA CATGGGCATC 
ACTCTGCACC TCTTGTTGCA CTCAGATCGC GACCCCATTC CAGACGTTCC TGCTATTTAC 
TTTATAATGC CCACAGAGGA GAACATCGAC AGGATATGTC AAGACCTCCG AAACCAACTA 
TATGAGTCCT ACTATTTGAA CTTCATCTCC GCAATTTCCA GAAGCAAATT GGAGGATATA 
GCAAGTGCTG CGCTTGCGGC TAATGCAGTA AACCAGGTGA CCAAAGTATT TGATCAGTAT 
TTGAACTTCA TCACACTTGA GGATGATATG TTCATTCTCT GTAATCAAAA CAAAGAGCAC 
ATCTCCTACC ATGCAATCAA TAAGCCAGAT ATCATGGACA CAGACATGGA GGGTATAATG 
GACACAATAG TGGATAGTCT CTTCTGCTTC TTTGTTACTC TTGGTGCTGT CCCAATAATT 
CGATGTCCAA GAGGGAATGC TGCCGAAATG GTAGCAGTGA AACTTGACAA GAAACTGAGA 
GAGAATTTGA GAGACGCTCG TAACAGCCTG TTCACCGGAG ACACCATGGG CACTGGCCAG 
TTCAGTTTCC AGAGGCCGTT GTTTGTTCTT GCGGATCGTA ACCTGGATCT TGCGACTCCT 
CTCCACCACA CATGGACGTA TCAGGCTCTA ATTCATGATG TGCTGGACTT CCACCTGAAC 
CGAGTGAGTG TGGACGAGTC TCATGGGTCA GAGGCCAGTC CAGCCGGAGC ACGACCCAAA 
AAGAAAAACA AGAAGAGCTA TGATCTCACT GCTGCTGACA AGTTTTGGCA AAAACACAAA 
GGAAGCCCAT TCCCAGAGGT TGCAGAAGCT GTGCAGGAGG AACTGGACAC ATATCGAGCC 
CAAGAGGATG AGGTTAAACG GCTCAAGAGC ATCATGGGCT TAGAAGGGGA GGACGACGGA 
GCCATTAGTA TGTTGTCAGA TAACACTGCC AAACTTACCT CAGCCGTCAG TTCTCTTCCT 
GAGCTCCTGG AAAAGAAGAG ACTGATTGAC CTCCACACCA ACGTTGCCAC AGCAGTTCTG 
GACCACATCA AGAGCCGTAA GCTTGACGTG TACTTCGAGT ACGAGGAGAA GCTCATGAGC 
AAATCCACAC TCGATAAATC CCTGCTGGAC ATTATCAGTG ACCCTGATGC TGGAACGGCA 
GAGGATAAGA TGAGGCTGTT TCTGATCTAC TACATCACTT CCCAGCAGCC CCCATCGGAG 
GCTGATCTAG AACAGTACAA GAAAGCCATG GTTGATGCAG GCTGCGACCT ATCCCCACTT 
AACTACATTA AACAGTGGAA AGCTTTCACC AAGATGGCTG CGACGCCAGC CAATTACGGG 
AGCAGTGGAG TGAAACCTAT GGGACTGTTC TCAAGGGTGA TGAACACCGG CTCCCAGTTT 
GTGATGGAGG GAGTGAAAAA TCTGGTCTTG AAACAGCATA ACCTACCGGT TACTCGCATC 
TTGGACAACT TGATGGAAAT GAAGTCAAAC CCAGAGACTG ATGACTACAG ATACTTTGAT 
CCCAAGATGC TTCGTGGAAG CGAGAGCTCC ATTCCGAGGA ACAAGAACCC CTTTCAGGAG 
GCTATCGTCT TTGTTGTTGG AGGAGGCAAT TACATAGAGT ATCAAAATCT TGTTGACTAC 
ACAAAGACCA GGCAAGGAAA GCGGATTCTT TATGGCTGTA GTGAGCTGTT CAATGCTGCC 
CAGTTCATTA AACAGCTCTC TCAGCTTGGT CAGAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

The Sec domain protein Scfd1 facilitates trafficking of ECM components during chondrogenesis.
Developmental biology421(1)8-15(2017 Jan)
Hou N,Yang Y,Scott IC,Lou X

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

The BH3-only SNARE BNip1 mediates photoreceptor apoptosis in response to vesicular fusion defects.
Developmental cell25(4)374-87(2013 May)
Nishiwaki Y,Yoshizawa A,Kojima Y,Oguri E,Nakamura S,Suzuki S,Yuasa-Kawada J,Kinoshita-Kawada M,Mochizuki T,Masai I

Simplet controls cell proliferation and gene transcription during zebrafish caudal fin regeneration.
Developmental biology325(2)329-40(2009 Jan)
Kizil C,Otto GW,Geisler R,N?sslein-Volhard C,Antos CL