pdx1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	pdx1
Entrez Gene ID	30721
Full Name	pancreatic and duodenal homeobox 1
Synonyms	ipf1,pdx-1,zgc:100874
General protein information	Preferred Names pancreatic and duodenal homeobox 1 Names pancreas/duodenum homeobox protein 1 insulin promoter factor 1, homeodomain transcription factor pancreatic and duodenal homeobox gene 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_131443.3	NP_571518.2	pancreas/duodenum homeobox protein 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	PDX1	NP_001179065.1
	PDX1	XP_001096758.1
Xenopus tropicalis (tropical clawed frog)	pdx1	XP_002934065.1
Canis lupus familiaris (dog)	PDX1	XP_543155.3
Mus musculus (house mouse)	Pdx1	NP_032840.1
Pan troglodytes (chimpanzee)	PDX1	NP_001074947.1
Danio rerio (zebrafish)	pdx1	NP_571518.2
Homo sapiens (human)	PDX1	NP_000200.1
Rattus norvegicus (Norway rat)	Pdx1	NP_074043.3
Gallus gallus (chicken)	PDX1	XP_001234636.2

Gene Ontology

The following pdx1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pdx1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa35850	NM_131443.3 Latest version!	Danio rerio pancreatic and duodenal homeobox 1 (pdx1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pdx1 ( NM_131443.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571518.2 Danio rerio pancreatic and duodenal homeobox 1 (pdx1), mRNA.

Price & Availability↑

CloneID	ODa35850
Clone ID Related Accession (Same CDS sequence)	NM_131443.3
Accession Version	NM_131443.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-14
Organism	Danio rerio(zebrafish)
Product	pancreas/duodenum homeobox protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC078192.1. On Aug 31, 2012 this sequence version replaced NM_131443.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC078192.1, AF036325.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END##

ATGAATCGGG AAGAGCATTA CTATCCGCCT AACCACCTGT ACAAGGACTC TTGTGCCTTC 
CAGAGACACC CCAACGAAGA CTACAGCCAA AACCCTCCAC CGTGTCTTTA TATGAGACAG 
GCACATTCAG TATACGCCTC ACCATTGGGC GCACAGGACC AGCCAAATCT TACCGACATT 
ACTTCTTATA ACATGTCGAG CCGGGATGAT CTGGCAGGGC CTCATCTTCA CCTTCCCCAA 
ACTTCACAGA CATCTCTACA GTCGCTCGGG GGTTACGGAG ACTCTCTGGA CCTCTGCGGG 
GATCGGAACA GATACCATCT CCCATTTCCG TGGATGAAGT CAACCAAATC TCACACGCAC 
GCATGGAAAG GACAGTGGAC AGGCCCTTAT ATGGTCGAGG CCGAGGAGAA CAAGCGCACG 
CGCACAGCTT ACACCAGAGC GCAGCTGCTC GAGCTCGAGA AAGAGTTTCT CTTCAACAAA 
TACATCTCGC GCCCGCGCCG CGTTGAACTC GCACTCACCC TCAGCCTCAC CGAGAGACAC 
ATCAAGATCT GGTTTCAGAA CCGACGCATG AAGTGGAAAA AAGAGGAGGA CAAGAGGAGA 
GCCAGGGGAG TGGATCCCGA ACAGGACTCC TCCATCACGT CCGGGGATCT TAAGGATGAA 
TCTTGCGTCG GCACGGCAAC ACTTGCGGGA CCCCCTTCCC CCCTGCACCC GCATGCACCT 
TCAGTCCAAC AAGACTCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571518.2
CDS	91..831
Translation	MNREEHYYPPNHLYKDSCAFQRHPNEDYSQNPPPCLYMRQAHSVYASPLGAQDQPNLTDITSYNMSSRDDLAGPHLHLPQTSQTSLQSLGGYGDSLDLCGDRNRYHLPFPWMKSTKSHTHAWKGQWTGPYMVEAEENKRTRTAYTRAQLLELEKEFLFNKYISRPRRVELALTLSLTERHIKIWFQNRRMKWKKEEDKRRARGVDPEQDSSITSGDLKDESCVGTATLAGPPSPLHPHAPSVQQDS

Target ORF information:

RefSeq Version	NM_131443.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio pancreatic and duodenal homeobox 1 (pdx1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131443.3

ORF Insert Sequence:

ATGAATCGGG AAGAGCATTA CTATCCGCCT AACCACCTGT ACAAGGACTC TTGTGCCTTC 
CAGAGACACC CCAACGAAGA CTACAGCCAA AACCCTCCAC CGTGTCTTTA TATGAGACAG 
GCACATTCAG TATACGCCTC ACCATTGGGC GCACAGGACC AGCCAAATCT TACCGACATT 
ACTTCTTATA ACATGTCGAG CCGGGATGAT CTGGCAGGGC CTCATCTTCA CCTTCCCCAA 
ACTTCACAGA CATCTCTACA GTCGCTCGGG GGTTACGGAG ACTCTCTGGA CCTCTGCGGG 
GATCGGAACA GATACCATCT CCCATTTCCG TGGATGAAGT CAACCAAATC TCACACGCAC 
GCATGGAAAG GACAGTGGAC AGGCCCTTAT ATGGTCGAGG CCGAGGAGAA CAAGCGCACG 
CGCACAGCTT ACACCAGAGC GCAGCTGCTC GAGCTCGAGA AAGAGTTTCT CTTCAACAAA 
TACATCTCGC GCCCGCGCCG CGTTGAACTC GCACTCACCC TCAGCCTCAC CGAGAGACAC 
ATCAAGATCT GGTTTCAGAA CCGACGCATG AAGTGGAAAA AAGAGGAGGA CAAGAGGAGA 
GCCAGGGGAG TGGATCCCGA ACAGGACTCC TCCATCACGT CCGGGGATCT TAAGGATGAA 
TCTTGCGTCG GCACGGCAAC ACTTGCGGGA CCCCCTTCCC CCCTGCACCC GCATGCACCT 
TCAGTCCAAC AAGACTCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.