ek1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ek1
Entrez Gene ID	30311
Full Name	eph-like kinase 1
Synonyms	epha3,etID309879.24,rtk7,zgc:100780
General protein information	Preferred Names eph-like kinase 1 Names ephrin type-A receptor 3 eph-like receptor tyrosine kinase 7 epha4d tyrosine-protein kinase receptor ZEK1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_131095.1	NP_571170.1	ephrin type-A receptor 3 precursor

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	ek1	NP_571170.1
Danio rerio (zebrafish)	epha4b	NP_705944.1
Xenopus tropicalis (tropical clawed frog)	LOC100494929	XP_004917824.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Conservation and early expression of zebrafish tyrosine kinases support the utility of zebrafish as a model for tyrosine kinase biology.
Challa AK, Chatti K
Zebrafish10(3)264-74(2013 Sep)

Integrity of the midbrain region is required to maintain the diencephalic-mesencephalic boundary in zebrafish no isthmus/pax2.1 mutants.
Scholpp S, Brand M
Developmental dynamics : an official publication of the American Association of Anatomists228(3)313-22(2003 Nov)

Characterisation of five novel zebrafish Eph-related receptor tyrosine kinases suggests roles in patterning the neural plate.
Cooke JE, Xu Q, Wilson SW, Holder N
Development genes and evolution206(8)515-531(1997 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ek1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ek1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa01248	NM_131095.1 Latest version!	Danio rerio eph-like kinase 1 (ek1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ek1 ( NM_131095.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571170.1 Danio rerio eph-like kinase 1 (ek1), mRNA.

Price & Availability↑

CloneID	ODa01248
Clone ID Related Accession (Same CDS sequence)	NM_131095.1
Accession Version	NM_131095.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2946bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-23
Organism	Danio rerio(zebrafish)
Product	ephrin type-A receptor 3 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U89295.1. On Jun 29, 2012 this sequence version replaced NM_001009911.1. ##Evidence-Data-START## Transcript exon combination :: U89295.1, BC162500.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168447, SAMEA4476733 [ECO:0000348] ##Evidence-Data-END##

ATGGCTCTCT TTAGGATTTA CTCTTTCCTT GCTCCTTTTC ACATACTAGT GCTGTGTCAA 
GCCTTGAGGA ATTATCCAGA TAATGAAGTT ACCCTGCTTG ACTCCATGTC CGCCCCAGGT 
GATCTGGGTT GGGAAGCGTA TCCATCAGAG GGGTGGGAGG AGATTAGTGT TATGGATGAG 
AGGAACATAC CTATGAGGAC TTACCAGGTA TGCAACGTAA TGGAAGCCAA TCAGAACAAC 
TGGTTGCGTA CTGGACTGAT TCAACGAGAA GGTGCTCAGC GCGTCTATGT GGAGATCAAA 
TTTACTCTGC GTGACTGCAA CAGCCTGCCG GGAGTCCCTG GAACCTGTAA GGAGACGTTT 
AACGTGTACT ACCACGAATC CAACAATGCA GTTGCCGCAC CTTTGCGGCA CATTCGTGAG 
AGCCAGTACA TAAAGATCGA CACTATAGCA GCTGATGAGA GCTTCACACA GACAGACGTT 
GGGGATCGTG TCATGAAGTT AAACACAGAA GTACGAGATA TCAGTGGCCT CAGTAAGAGA 
GGCTTGTATC TGGCATTTCA GGACCTGGGT GCTTGCATTG CTCTGGTGTC GGTGAGAGTC 
TTTTATAAAC GCTGTCCGTT GGCGGTGCTC AATCTGGCTC GGTTTCCAGA CACAGTGACG 
GGTGGTGATT CAGCGCTGGT GGAAGTGCGT GGGACTTGTG TGGAAGATGC AGAAGAGCTG 
GAGGGACCAC GGATGTTTTG CAGCGCAGAC GGAGGTTGGC TGGTGCCCAT CGGCCGCTGT 
GTTTGCAGGC CAGGTTTCGA GGAAGTGGAT GGACACTGTC AACCATGTCG GTCAGGCTTC 
TACAAGGCCT CAGCTATGGA TGCGTACTGT GTCAAGTGTC CTCCACACAG CTACTCTCAC 
CAGGACAAGG CCTCTGAGTG TGTATGTGAA CGAGGTTTCT ATCGAGCAGA ATCAGACCCT 
CGCTCTATGG CCTGCACAAG ACCACCTTCA GCACCAGGAA ACCCGATTTC GATGGTGAAC 
GAGACAGCGG TGACTCTGGA ATGGAGTCCT CCCCGAGACA GTGGTGGACG TGGAGATGTC 
AGCTATAGTG TTCACTGCAG AAAGTGCTCT GGCGAAACAG GGGCGTCAGA GAGGTGTGTT 
CCCTGTGGCA GCGGCGCACA CTTCAACCCC AGACAGTTCG GCTTGACGCA CCCCAGAGTT 
CTGGTCACTG AACTGCAGCC ACACACCAAT TACACCTTCA GCGTTGAAGC CCTGAATGGA 
GTCTCAGATC TGAGCCCCAG TCCACGTCAG CTGGTGTCTG TCAACGTCAC CACCAGTCAG 
ACAGTGAGCG TCATCTTAAA GGAAAGGAAG GGCACTGATA GTGTGACGCT GGCATGGCAG 
GGTCCAGAAC CTGTTGACGG GACCGTGGTG GAATATGAAG TCACCTATTA TGAAAAGAAC 
CAGCAGGATC AGAACTACAC TGTCCTCAAG ACAAAATCCA ACAGCATGAC GGTGGACGGG 
CTTAAACCGG GAACTACATA CATCTTCCGG GTTCGAGCAC GTACTGATGG AGGATATGGG 
AACTATAAGG GAGAGATTGA GTTGGAGACG AGCCACGAGG ACATGCTGGC AGTCGGAGAT 
CCAAACCAGC AAACCATACT GGCTATTTCA GTGGCCGGCG GTGCAGTTCT CCTGGTCCTG 
CTTGTGGCCT GCTTCATTGT TAGTGGACGG CGCTGTGGAT ATATTAAAGC AAAGCAAGAC 
CCTGAGGAGG AGAAAATGCA GTTTCAGCAT GGACGAGTCA AACTTCCTGA AACCCGTACG 
TACATTCACC CCCATACATA TGAAGACCCG AACCAGGCAG TGCGAGACTT CGCCAAAGAG 
ATTGAAGTCT CCAACATTCG GATTGAAAGA GTCATTGGGG CTGGTGAGTT CGGCGAGGTC 
TGCAGTGGCC GTCTGCGTCT GCCCAGTAAA AGAGAGATCC AGGTGGCCAT CAAGAGTCTG 
AAAGCTGGAT ATTCAGAACA CCAGAGACGC GACTTTCTGA GCGAGGCCAG CATTATGGGC 
CAGTTTGACC ACCCTAACAT CATTCGACTG GAAGGAGTTG TCACCAGATG TAAGCCTGTG 
ATGATAGTGA CAGAGTACAT GGAAAATGGA TCTCTGGACA CTTTTTTGAA GAAACACGAC 
GGGCAGTTCA CAGTGATCCA GCTGTTGGGA ATGCTGAGAG GGATCGCCGC CGGGATGCAG 
TACCTCTCCG AGATGAACTA CGTGCATCGG GATTTGGCAG CACGGAACAT TCTGGTAAAC 
AGAAACCTGG TGTGTAAAGT GTCTGACTTC GGGCTGTCGC GCGTGCTGGA GGACGATCCT 
GAAGCGGCAT ACACTACACG GGGTGGAAAG ATTCCCATTC GCTGGACGGC TCCAGAGGCC 
ATCACTTACA GGAAGTTTAC CTCAGCCAGT GATGTATGGA GTTACGGCAT TGTCATGTGG 
GAGGTGATAT CCTATGGGGA GCGACCGTAC TGGGAAATGT CCAATCAGGA TGTGATAAAA 
GCTGTAGATG AAGGCTACAG GCTTCCAGCC CCGATGGATT GTCCGGTGGT GCTTCACCAG 
CTCATGCTTG ACTGCTGGGA AAAGAACCGC AGCGATCGGC CTAAATTCGG GCAGATTGTC 
AACACTCTGG ACAGGCTGAT CCGAAATCCC AGCAGCCTAA AGCAGCTGGC CAACAGTGCG 
GTCTGGGAGG ATCCAGTGAC TCCTGAGGCG GCGGTCAACA CAGTTGAAGA CTGGCTGGAC 
TTGATTAAAA TGGGTCAGTA CAAAGAACAC TTCTCCAGCG CCGGCTACGT TACCCTGGAC 
TCAGTTCTGT ATGTCAGCAG CAGTGAATTG GACAAGATGG GTGTTGAGCT GGCAGGACAT 
CAGAAGAAGA TCCTGTCCAG CATCCAGTGT CTTCAGGCAC ATCACGGGAC TCAAGTTCAG 
GTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571170.1
CDS	126..3071
Translation	MALFRIYSFLAPFHILVLCQALRNYPDNEVTLLDSMSAPGDLGWEAYPSEGWEEISVMDERNIPMRTYQVCNVMEANQNNWLRTGLIQREGAQRVYVEIKFTLRDCNSLPGVPGTCKETFNVYYHESNNAVAAPLRHIRESQYIKIDTIAADESFTQTDVGDRVMKLNTEVRDISGLSKRGLYLAFQDLGACIALVSVRVFYKRCPLAVLNLARFPDTVTGGDSALVEVRGTCVEDAEELEGPRMFCSADGGWLVPIGRCVCRPGFEEVDGHCQPCRSGFYKASAMDAYCVKCPPHSYSHQDKASECVCERGFYRAESDPRSMACTRPPSAPGNPISMVNETAVTLEWSPPRDSGGRGDVSYSVHCRKCSGETGASERCVPCGSGAHFNPRQFGLTHPRVLVTELQPHTNYTFSVEALNGVSDLSPSPRQLVSVNVTTSQTVSVILKERKGTDSVTLAWQGPEPVDGTVVEYEVTYYEKNQQDQNYTVLKTKSNSMTVDGLKPGTTYIFRVRARTDGGYGNYKGEIELETSHEDMLAVGDPNQQTILAISVAGGAVLLVLLVACFIVSGRRCGYIKAKQDPEEEKMQFQHGRVKLPETRTYIHPHTYEDPNQAVRDFAKEIEVSNIRIERVIGAGEFGEVCSGRLRLPSKREIQVAIKSLKAGYSEHQRRDFLSEASIMGQFDHPNIIRLEGVVTRCKPVMIVTEYMENGSLDTFLKKHDGQFTVIQLLGMLRGIAAGMQYLSEMNYVHRDLAARNILVNRNLVCKVSDFGLSRVLEDDPEAAYTTRGGKIPIRWTAPEAITYRKFTSASDVWSYGIVMWEVISYGERPYWEMSNQDVIKAVDEGYRLPAPMDCPVVLHQLMLDCWEKNRSDRPKFGQIVNTLDRLIRNPSSLKQLANSAVWEDPVTPEAAVNTVEDWLDLIKMGQYKEHFSSAGYVTLDSVLYVSSSELDKMGVELAGHQKKILSSIQCLQAHHGTQVQV

Target ORF information:

RefSeq Version	NM_131095.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio eph-like kinase 1 (ek1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131095.1

ORF Insert Sequence:

ATGGCTCTCT TTAGGATTTA CTCTTTCCTT GCTCCTTTTC ACATACTAGT GCTGTGTCAA 
GCCTTGAGGA ATTATCCAGA TAATGAAGTT ACCCTGCTTG ACTCCATGTC CGCCCCAGGT 
GATCTGGGTT GGGAAGCGTA TCCATCAGAG GGGTGGGAGG AGATTAGTGT TATGGATGAG 
AGGAACATAC CTATGAGGAC TTACCAGGTA TGCAACGTAA TGGAAGCCAA TCAGAACAAC 
TGGTTGCGTA CTGGACTGAT TCAACGAGAA GGTGCTCAGC GCGTCTATGT GGAGATCAAA 
TTTACTCTGC GTGACTGCAA CAGCCTGCCG GGAGTCCCTG GAACCTGTAA GGAGACGTTT 
AACGTGTACT ACCACGAATC CAACAATGCA GTTGCCGCAC CTTTGCGGCA CATTCGTGAG 
AGCCAGTACA TAAAGATCGA CACTATAGCA GCTGATGAGA GCTTCACACA GACAGACGTT 
GGGGATCGTG TCATGAAGTT AAACACAGAA GTACGAGATA TCAGTGGCCT CAGTAAGAGA 
GGCTTGTATC TGGCATTTCA GGACCTGGGT GCTTGCATTG CTCTGGTGTC GGTGAGAGTC 
TTTTATAAAC GCTGTCCGTT GGCGGTGCTC AATCTGGCTC GGTTTCCAGA CACAGTGACG 
GGTGGTGATT CAGCGCTGGT GGAAGTGCGT GGGACTTGTG TGGAAGATGC AGAAGAGCTG 
GAGGGACCAC GGATGTTTTG CAGCGCAGAC GGAGGTTGGC TGGTGCCCAT CGGCCGCTGT 
GTTTGCAGGC CAGGTTTCGA GGAAGTGGAT GGACACTGTC AACCATGTCG GTCAGGCTTC 
TACAAGGCCT CAGCTATGGA TGCGTACTGT GTCAAGTGTC CTCCACACAG CTACTCTCAC 
CAGGACAAGG CCTCTGAGTG TGTATGTGAA CGAGGTTTCT ATCGAGCAGA ATCAGACCCT 
CGCTCTATGG CCTGCACAAG ACCACCTTCA GCACCAGGAA ACCCGATTTC GATGGTGAAC 
GAGACAGCGG TGACTCTGGA ATGGAGTCCT CCCCGAGACA GTGGTGGACG TGGAGATGTC 
AGCTATAGTG TTCACTGCAG AAAGTGCTCT GGCGAAACAG GGGCGTCAGA GAGGTGTGTT 
CCCTGTGGCA GCGGCGCACA CTTCAACCCC AGACAGTTCG GCTTGACGCA CCCCAGAGTT 
CTGGTCACTG AACTGCAGCC ACACACCAAT TACACCTTCA GCGTTGAAGC CCTGAATGGA 
GTCTCAGATC TGAGCCCCAG TCCACGTCAG CTGGTGTCTG TCAACGTCAC CACCAGTCAG 
ACAGTGAGCG TCATCTTAAA GGAAAGGAAG GGCACTGATA GTGTGACGCT GGCATGGCAG 
GGTCCAGAAC CTGTTGACGG GACCGTGGTG GAATATGAAG TCACCTATTA TGAAAAGAAC 
CAGCAGGATC AGAACTACAC TGTCCTCAAG ACAAAATCCA ACAGCATGAC GGTGGACGGG 
CTTAAACCGG GAACTACATA CATCTTCCGG GTTCGAGCAC GTACTGATGG AGGATATGGG 
AACTATAAGG GAGAGATTGA GTTGGAGACG AGCCACGAGG ACATGCTGGC AGTCGGAGAT 
CCAAACCAGC AAACCATACT GGCTATTTCA GTGGCCGGCG GTGCAGTTCT CCTGGTCCTG 
CTTGTGGCCT GCTTCATTGT TAGTGGACGG CGCTGTGGAT ATATTAAAGC AAAGCAAGAC 
CCTGAGGAGG AGAAAATGCA GTTTCAGCAT GGACGAGTCA AACTTCCTGA AACCCGTACG 
TACATTCACC CCCATACATA TGAAGACCCG AACCAGGCAG TGCGAGACTT CGCCAAAGAG 
ATTGAAGTCT CCAACATTCG GATTGAAAGA GTCATTGGGG CTGGTGAGTT CGGCGAGGTC 
TGCAGTGGCC GTCTGCGTCT GCCCAGTAAA AGAGAGATCC AGGTGGCCAT CAAGAGTCTG 
AAAGCTGGAT ATTCAGAACA CCAGAGACGC GACTTTCTGA GCGAGGCCAG CATTATGGGC 
CAGTTTGACC ACCCTAACAT CATTCGACTG GAAGGAGTTG TCACCAGATG TAAGCCTGTG 
ATGATAGTGA CAGAGTACAT GGAAAATGGA TCTCTGGACA CTTTTTTGAA GAAACACGAC 
GGGCAGTTCA CAGTGATCCA GCTGTTGGGA ATGCTGAGAG GGATCGCCGC CGGGATGCAG 
TACCTCTCCG AGATGAACTA CGTGCATCGG GATTTGGCAG CACGGAACAT TCTGGTAAAC 
AGAAACCTGG TGTGTAAAGT GTCTGACTTC GGGCTGTCGC GCGTGCTGGA GGACGATCCT 
GAAGCGGCAT ACACTACACG GGGTGGAAAG ATTCCCATTC GCTGGACGGC TCCAGAGGCC 
ATCACTTACA GGAAGTTTAC CTCAGCCAGT GATGTATGGA GTTACGGCAT TGTCATGTGG 
GAGGTGATAT CCTATGGGGA GCGACCGTAC TGGGAAATGT CCAATCAGGA TGTGATAAAA 
GCTGTAGATG AAGGCTACAG GCTTCCAGCC CCGATGGATT GTCCGGTGGT GCTTCACCAG 
CTCATGCTTG ACTGCTGGGA AAAGAACCGC AGCGATCGGC CTAAATTCGG GCAGATTGTC 
AACACTCTGG ACAGGCTGAT CCGAAATCCC AGCAGCCTAA AGCAGCTGGC CAACAGTGCG 
GTCTGGGAGG ATCCAGTGAC TCCTGAGGCG GCGGTCAACA CAGTTGAAGA CTGGCTGGAC 
TTGATTAAAA TGGGTCAGTA CAAAGAACAC TTCTCCAGCG CCGGCTACGT TACCCTGGAC 
TCAGTTCTGT ATGTCAGCAG CAGTGAATTG GACAAGATGG GTGTTGAGCT GGCAGGACAT 
CAGAAGAAGA TCCTGTCCAG CATCCAGTGT CTTCAGGCAC ATCACGGGAC TCAAGTTCAG 
GTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Conservation and early expression of zebrafish tyrosine kinases support the utility of zebrafish as a model for tyrosine kinase biology.
Zebrafish10(3)264-74(2013 Sep)
Challa AK,Chatti K

Integrity of the midbrain region is required to maintain the diencephalic-mesencephalic boundary in zebrafish no isthmus/pax2.1 mutants.
Developmental dynamics : an official publication of the American Association of Anatomists228(3)313-22(2003 Nov)
Scholpp S,Brand M

Characterisation of five novel zebrafish Eph-related receptor tyrosine kinases suggests roles in patterning the neural plate.
Development genes and evolution206(8)515-531(1997 May)
Cooke JE,Xu Q,Wilson SW,Holder N

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ek1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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