pes cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	pes
Entrez Gene ID	30228
Full Name	pescadillo
General protein information	Preferred Names pescadillo Names pescadillo etID309792.5
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_131030.3	NP_571105.3	pescadillo

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Pes1	NP_075027.1
Kluyveromyces lactis	KLLA0D15268g	XP_453738.1
Eremothecium gossypii	AGOS_AAR094W	NP_982635.1
Magnaporthe oryzae (rice blast fungus)	MGG_01183	XP_003714067.1
Bos taurus (cattle)	PES1	NP_001179229.1
Caenorhabditis elegans (roundworm)	lpd-7	NP_498661.1
Danio rerio (zebrafish)	pes	NP_571105.2
Drosophila melanogaster (fruit fly)	CG4364	NP_609305.1
Anopheles gambiae (African malaria mosquito)	PESC_ANOGA	XP_308647.4
Saccharomyces cerevisiae (baker's yeast)	NOP7	NP_011617.1
Pan troglodytes (chimpanzee)	PES1	NP_001233294.1
	PES1	XP_001110399.2
Rattus norvegicus (Norway rat)	Pes1	NP_001037693.1
Schizosaccharomyces pombe (fission yeast)	ppp1	NP_596543.1
Neurospora crassa	NCU00925	XP_964751.1
Homo sapiens (human)	PES1	NP_055118.1
Canis lupus familiaris (dog)	PES1	XP_543480.2
Gallus gallus (chicken)	PES1	XP_001234133.3
Arabidopsis thaliana (thale cress)	AT5G14520	NP_196956.1
Oryza sativa (rice)	Os03g0699200	NP_001050986.1
Xenopus tropicalis (tropical clawed frog)	pes1	NP_989410.1

Gene Ontology
Bibliography

Related articles in PubMed

Cross talk between TP53 and c-Myc in the pathophysiology of Diamond-Blackfan anemia: Evidence from RPL11-deficient in?vivo and in?vitro models.
Chakraborty A, Uechi T, Nakajima Y, Gazda HT, O'Donohue MF, Gleizes PE, Kenmochi N
Biochemical and biophysical research communications495(2)1839-1845(2018 Jan)

Number of inadvertent RNA targets for morpholino knockdown in Danio rerio is largely underestimated: evidence from the study of Ser/Arg-rich splicing factors.
Joris M, Schloesser M, Baurain D, Hanikenne M, Muller M, Motte P
Nucleic acids research45(16)9547-9557(2017 Sep)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Zebrafish midbrain slow-amplifying progenitors exhibit high levels of transcripts for nucleotide and ribosome biogenesis.
Recher G, Jouralet J, Brombin A, Heuz? A, Mugniery E, Hermel JM, Desnoulez S, Savy T, Herbomel P, Bourrat F, Peyri?ras N, Jamen F, Joly JS
Development (Cambridge, England)140(24)4860-9(2013 Dec)

Isolation and characterization of a carrot nucleolar protein with structural and sequence similarity to the vertebrate PESCADILLO protein.
Ueda K, Xu ZJ, Miyagi N, Ono M, Wabiko H, Masuda K, Inoue M
Plant science : an international journal of experimental plant biology20883-92(2013 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Knockdown or mutation of either rpl3 or pes resulted in impaired expansion of pancreatic progenitor cells.
Title: Ribosomal biogenesis genes play an essential and p53-independent role in zebrafish pancreas development.

neural precursors require pes function to progress through the cell cycle and produce oligodendrocyte progenitor cells and for oligodendrocyte differentiation
Title: Mutation of pescadillo disrupts oligodendrocyte formation in zebrafish.

The following pes gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pes cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa39194	NM_131030.3 Latest version!	Danio rerio pescadillo (pes), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pes ( NM_131030.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571105.3 Danio rerio pescadillo (pes), mRNA.

Price & Availability↑

CloneID	ODa39194
Clone ID Related Accession (Same CDS sequence)	NM_131030.3
Accession Version	NM_131030.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Danio rerio(zebrafish)
Product	pescadillo
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU459127.6, BX470167.7 and BC049339.1. On Aug 27, 2015 this sequence version replaced NM_131030.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC049339.1, U77627.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCGGAT TACAAAAGAA GAAGTATGAG AGTGGGTCAG CCACTAACTA CATCACCAGG 
AATAAAGCTC GTAAGAAGCT GAGTCTGAGT CTGGCTGATT TCAGGCGGTT ATGCATATTA 
AAAGGAATTT ATCCTCATGA GCCCAAACAC AAAAAGAAGG TCAACAAGGG CTCGACGGCT 
GCTAGAACTT ACTATCTGCT GAAAGACATC CGCTTCCTGC TGCATGAACC CATCGTTGGC 
AAATTCAGAG AGTATAAGAT CTTTGTCCGG AAGCTGCGGA AAGCGTATGG GAAGGCTGAG 
TGGAGCGCAG TGGAGAGACT GAAAGAAAAC AAGCCTGGCT ACAAACTGGA CCACATCATC 
AAGGAGAGGT ATCCCACGTT CATTGATGCT CTCCGAGATG TGGACGATGC CCTTTCTATG 
TGTTTTCTGT TCTCTACATT CGCTCGTACT GGAAAATGCC ACGTCCAGAC AATTCAGCTG 
TGTCGGAGAC TCAGTGTTGA GTGGATGAAC TACATCATCT CCTCACGCTC CCTAAGAAAG 
GTTTTTCTTT CCATTAAGGG TATTTACTAT CAGGCAGAGG TTTTGGGACA GACCATCACA 
TGGATCGTAC CATATCAGTT TGCCCATAAT CATCCTACAG ATGTGGACTA CAGAGTTATG 
GCCACATTCA CAGAGCTCTA CACAACACTG CTCGGCTTCG TTAACTTCCG CCTTTATCAG 
ACTCTCAACC TTGTCTACCC GCCCAAGCTT GATGGACAAG GAGAGATCAG TCTCAAAGCA 
GAGTTTGAAG AAGATTACGC CCTGGAATCT GAAAGCTACA CTGAGAAACT GTCAGCATTG 
AGTGCCAGTC TGGCTCGTAT GGTGGCGTCA GTGGAGGAGG AGGAGGCAGA GCTGGACCAC 
TTCCCTACTG AAGGGGAGGA CCAGGAGAAA ATGGAGGTGA GGGAGAAGAT GGAGCAGCAG 
CAGAGCAAAC AGAAGAAACT CTTCGAAGGC CTCAAGTTTT TCCTAAACAG AGAAGTTCCC 
AGGGAATCGT TGGCGTTCGT GATCAGGTGT TTTGGTGGAG AGGTGTCTTG GGATAAATCC 
CTGTGCATCG GCAGCACGTA TGAAGCCACA GACGAAACAA TCACGCATCA CATCGTGGAC 
AGACCCAGCA TGGACAAACA GTATATCAAC AGATACTACA TTCAGCCCCA GTGGGTGTAT 
GACTCTGTCA ATGCTAAGAT ACAGTTACCT GTGGAGGAGT ATTTTCTGGG AGTCACACTG 
CCGCCTCATC TGTCACCGTT TGTGGAGGAG ACTGAAGGAG ATTATGTGCC GCCAGAGAAA 
CTCAAGCTCA TGGCCCTGCA GAGAGGAGAG AAACCACAAG CAGAAGAAGA CGAGGAAGAG 
GAGGGAGAAG AAGAAGAGGA TGATGAAGAA GATGAAGAGG ACGATGAGCA GAGTGAGGAT 
GAAGAGGAAG CAGAAGAAGA AGCAAATCTA GCTGAAATGG AGGAGAAGAG ATCTCAGGGA 
AAGAGCCTCT CTGTAAAGGT GACTCCTGGT AAAGCCAAAG CTGAGAACCG TGCGAGAGCA 
GCAGAGGAGG AGAAAGCAGA GGAGAAGAGA CTGGCCATTA TGATGATGAA GAAGAAAGAG 
AAATATCTCT ATGACAAGAT CATGTTCGGC AAGAAGAGAA AAGTCAGAGA GGCTAACAAA 
CTGGCTGCCA AGAGGAAAGC TCACGATGAC GCCAGCAAAG CAGACAAGAA GAAGAAGAAG 
AAGAAGTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571105.3
CDS	134..1885
Translation	MGGLQKKKYESGSATNYITRNKARKKLSLSLADFRRLCILKGIYPHEPKHKKKVNKGSTAARTYYLLKDIRFLLHEPIVGKFREYKIFVRKLRKAYGKAEWSAVERLKENKPGYKLDHIIKERYPTFIDALRDVDDALSMCFLFSTFARTGKCHVQTIQLCRRLSVEWMNYIISSRSLRKVFLSIKGIYYQAEVLGQTITWIVPYQFAHNHPTDVDYRVMATFTELYTTLLGFVNFRLYQTLNLVYPPKLDGQGEISLKAEFEEDYALESESYTEKLSALSASLARMVASVEEEEAELDHFPTEGEDQEKMEVREKMEQQQSKQKKLFEGLKFFLNREVPRESLAFVIRCFGGEVSWDKSLCIGSTYEATDETITHHIVDRPSMDKQYINRYYIQPQWVYDSVNAKIQLPVEEYFLGVTLPPHLSPFVEETEGDYVPPEKLKLMALQRGEKPQAEEDEEEEGEEEEDDEEDEEDDEQSEDEEEAEEEANLAEMEEKRSQGKSLSVKVTPGKAKAENRARAAEEEKAEEKRLAIMMMKKKEKYLYDKIMFGKKRKVREANKLAAKRKAHDDASKADKKKKKKKC

Target ORF information:

RefSeq Version	NM_131030.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio pescadillo (pes), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131030.3

ORF Insert Sequence:

ATGGGCGGAT TACAAAAGAA GAAGTATGAG AGTGGGTCAG CCACTAACTA CATCACCAGG 
AATAAAGCTC GTAAGAAGCT GAGTCTGAGT CTGGCTGATT TCAGGCGGTT ATGCATATTA 
AAAGGAATTT ATCCTCATGA GCCCAAACAC AAAAAGAAGG TCAACAAGGG CTCGACGGCT 
GCTAGAACTT ACTATCTGCT GAAAGACATC CGCTTCCTGC TGCATGAACC CATCGTTGGC 
AAATTCAGAG AGTATAAGAT CTTTGTCCGG AAGCTGCGGA AAGCGTATGG GAAGGCTGAG 
TGGAGCGCAG TGGAGAGACT GAAAGAAAAC AAGCCTGGCT ACAAACTGGA CCACATCATC 
AAGGAGAGGT ATCCCACGTT CATTGATGCT CTCCGAGATG TGGACGATGC CCTTTCTATG 
TGTTTTCTGT TCTCTACATT CGCTCGTACT GGAAAATGCC ACGTCCAGAC AATTCAGCTG 
TGTCGGAGAC TCAGTGTTGA GTGGATGAAC TACATCATCT CCTCACGCTC CCTAAGAAAG 
GTTTTTCTTT CCATTAAGGG TATTTACTAT CAGGCAGAGG TTTTGGGACA GACCATCACA 
TGGATCGTAC CATATCAGTT TGCCCATAAT CATCCTACAG ATGTGGACTA CAGAGTTATG 
GCCACATTCA CAGAGCTCTA CACAACACTG CTCGGCTTCG TTAACTTCCG CCTTTATCAG 
ACTCTCAACC TTGTCTACCC GCCCAAGCTT GATGGACAAG GAGAGATCAG TCTCAAAGCA 
GAGTTTGAAG AAGATTACGC CCTGGAATCT GAAAGCTACA CTGAGAAACT GTCAGCATTG 
AGTGCCAGTC TGGCTCGTAT GGTGGCGTCA GTGGAGGAGG AGGAGGCAGA GCTGGACCAC 
TTCCCTACTG AAGGGGAGGA CCAGGAGAAA ATGGAGGTGA GGGAGAAGAT GGAGCAGCAG 
CAGAGCAAAC AGAAGAAACT CTTCGAAGGC CTCAAGTTTT TCCTAAACAG AGAAGTTCCC 
AGGGAATCGT TGGCGTTCGT GATCAGGTGT TTTGGTGGAG AGGTGTCTTG GGATAAATCC 
CTGTGCATCG GCAGCACGTA TGAAGCCACA GACGAAACAA TCACGCATCA CATCGTGGAC 
AGACCCAGCA TGGACAAACA GTATATCAAC AGATACTACA TTCAGCCCCA GTGGGTGTAT 
GACTCTGTCA ATGCTAAGAT ACAGTTACCT GTGGAGGAGT ATTTTCTGGG AGTCACACTG 
CCGCCTCATC TGTCACCGTT TGTGGAGGAG ACTGAAGGAG ATTATGTGCC GCCAGAGAAA 
CTCAAGCTCA TGGCCCTGCA GAGAGGAGAG AAACCACAAG CAGAAGAAGA CGAGGAAGAG 
GAGGGAGAAG AAGAAGAGGA TGATGAAGAA GATGAAGAGG ACGATGAGCA GAGTGAGGAT 
GAAGAGGAAG CAGAAGAAGA AGCAAATCTA GCTGAAATGG AGGAGAAGAG ATCTCAGGGA 
AAGAGCCTCT CTGTAAAGGT GACTCCTGGT AAAGCCAAAG CTGAGAACCG TGCGAGAGCA 
GCAGAGGAGG AGAAAGCAGA GGAGAAGAGA CTGGCCATTA TGATGATGAA GAAGAAAGAG 
AAATATCTCT ATGACAAGAT CATGTTCGGC AAGAAGAGAA AAGTCAGAGA GGCTAACAAA 
CTGGCTGCCA AGAGGAAAGC TCACGATGAC GCCAGCAAAG CAGACAAGAA GAAGAAGAAG 
AAGAAGTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cross talk between TP53 and c-Myc in the pathophysiology of Diamond-Blackfan anemia: Evidence from RPL11-deficient in?vivo and in?vitro models.
Biochemical and biophysical research communications495(2)1839-1845(2018 Jan)
Chakraborty A,Uechi T,Nakajima Y,Gazda HT,O'Donohue MF,Gleizes PE,Kenmochi N

Number of inadvertent RNA targets for morpholino knockdown in Danio rerio is largely underestimated: evidence from the study of Ser/Arg-rich splicing factors.
Nucleic acids research45(16)9547-9557(2017 Sep)
Joris M,Schloesser M,Baurain D,Hanikenne M,Muller M,Motte P

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Zebrafish midbrain slow-amplifying progenitors exhibit high levels of transcripts for nucleotide and ribosome biogenesis.
Development (Cambridge, England)140(24)4860-9(2013 Dec)
Recher G,Jouralet J,Brombin A,Heuz? A,Mugniery E,Hermel JM,Desnoulez S,Savy T,Herbomel P,Bourrat F,Peyri?ras N,Jamen F,Joly JS

Isolation and characterization of a carrot nucleolar protein with structural and sequence similarity to the vertebrate PESCADILLO protein.
Plant science : an international journal of experimental plant biology20883-92(2013 Jul)
Ueda K,Xu ZJ,Miyagi N,Ono M,Wabiko H,Masuda K,Inoue M

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pes cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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