tle3a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tle3a
Entrez Gene ID	30206
Full Name	transducin-like enhancer of split 3a
Synonyms	grg2,gro2,groucho2,sb:eu753
General protein information	Preferred Names transducin-like enhancer of split 3a Names transducin-like enhancer protein 3-A protein groucho-2 groucho-related Grg2 groucho-related protein grg2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005159039.3	XP_005159096.1	protein groucho-2 isoform X1
XM_017351898.1	XP_017207387.1	protein groucho-2 isoform X1
XM_017351898.2	XP_017207387.1	protein groucho-2 isoform X1
NM_001313694.1	NP_001300623.1	transducin-like enhancer protein 3-A isoform 1
XM_005159041.3	XP_005159098.1	protein groucho-2 isoform X2
XM_005159041.4	XP_005159098.1	protein groucho-2 isoform X2
NM_131012.2	NP_571087.2	transducin-like enhancer protein 3-A isoform 2
XM_005159039.4	XP_005159096.1	protein groucho-2 isoform X1

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004280	XP_313201.4
	TLE3	XP_001086565.2
Danio rerio (zebrafish)	gro2	NP_571087.1
Drosophila melanogaster (fruit fly)	gro	NP_996298.1

Gene Ontology
Bibliography

Related articles in PubMed

Transcriptional and histological alterations in gonad of adult zebrafish after exposure to the synthetic progestin norgestrel.
Shi WJ, Zhao JL, Jiang YX, Huang GY, Liu YS, Zhang JN, Ying GG
Environmental toxicology and chemistry36(12)3267-3276(2017 Dec)

An Integrative Developmental Genomics and Systems Biology Approach to Identify an In Vivo Sox Trio-Mediated Gene Regulatory Network in Murine Embryos.
Lee WJ, Chatterjee S, Yap SP, Lim SL, Xing X, Kraus P, Sun W, Hu X, Sivakamasundari V, Chan HY, Kolatkar PR, Prabhakar S, Lufkin T
BioMed research international20178932583(2017)

Testing multiple hypotheses through IMP weighted FDR based on a genetic functional network with application to a new zebrafish transcriptome study.
Gui J, Greene CS, Sullivan C, Taylor W, Moore JH, Kim C
BioData mining817(2015)

Two zebrafish homologues of the Drosophila neurogenic gene groucho and their pattern of transcription during early embryogenesis.
W?lbeck C, Campos-Ortega JA
Development genes and evolution207(3)156-166(1997 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following tle3a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tle3a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa23208	NM_001313694.1 Latest version!	Danio rerio TLE family member 3, transcriptional corepressor a (tle3a), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa23209	NM_131012.2 Latest version!	Danio rerio TLE family member 3, transcriptional corepressor a (tle3a), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa23208	XM_005159039.4 Latest version!	Danio rerio transducin-like enhancer of split 3a (tle3a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa23208	XM_017351898.2 Latest version!	Danio rerio transducin-like enhancer of split 3a (tle3a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa23209	XM_005159041.4 Latest version!	Danio rerio transducin-like enhancer of split 3a (tle3a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
ODa23208	XM_017351898.1	Danio rerio transducin-like enhancer of split 3a (tle3a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa23209	XM_005159041.3	Danio rerio transducin-like enhancer of split 3a (tle3a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa23208	XM_005159039.3	Danio rerio transducin-like enhancer of split 3a (tle3a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tle3a ( NM_001313694.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001300623.1 Danio rerio TLE family member 3, transcriptional corepressor a (tle3a), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ODa23208
Clone ID Related Accession (Same CDS sequence)	XM_005159039.3 , XM_017351898.1 , XM_017351898.2 , NM_001313694.1 , XM_005159039.4
Accession Version	NM_001313694.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-07
Organism	Danio rerio(zebrafish)
Product	transducin-like enhancer protein 3-A isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT613405.2, BX324138.6 and EH525475.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC162533.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTATCCAC AGGGCCGGCA TCCGGCGCCC CACCAGCCTG GTCAGCCAGG CTTCAAATTC 
ACAGTGGCGG AGTCCTGTGA CAGGATCAAA GACGAATTTC AGTTCCTGCA AGCCCAATAT 
CACAGTCTCA AAGTGGAGTA CGACAAACTG GCCAATGAGA AGACCGAGAT GCAGCGCCAT 
TATGTCATGT ACTATGAGAT GTCCTATGGG CTGAACATTG AAATGCACAA ACAGACCGAG 
ATTGCCAAAA GGCTCAATGC GATTCTAGCC CAGATTATGC CTTTTCTGTC ACAAGAGCAC 
CAACAGCAAG TAGCACAAGC TGTTGAGCGT GCAAAACAAG TGACAATGAC CGAACTGAAT 
GCTATCATCG GGGTACGTGG ACTTCCCAAT CTGCCTCTCA CCCAGCAGCA GCTCCAAGCC 
CAGCACCTCT CTCACGCTGC CCACGGCCCC GTGGCTTTAC CTCCCCACCC TTCTGGCCTT 
CAGCCACCAG GAATCCCTCC GGTCACCGGC TCTGGCTCAG GCCTGCTGGC TCTAGGGGCC 
CTGGGAAGTC AGCCACACTT GCCGGTGAAG GATGAAAAAA ACCACCATGA CCTGGAGCAC 
CGAGAGAGCA CGAACAACTC CATCTCTCCA TCAGACAGCC TGCGCACAAC CAGTGAGAAG 
CACCGTGGCT CCTCCGATTA CAGCTTAGAC TCCAAAAAAC GCAAAGTGGA GGACAAGGAC 
AGCATGAGTC GATATGACAG TGATGGAGAC AAAAGTGATG ATTTGGTGGT TGATGTTTCC 
AATGAGGACC CTGCCACGCC CAGAGGCAGC CCCGCCCACT CGCCGCCAGA GAATGGTATA 
GATAAGCCCC GGCCAGCAAA AAAGGACACA CCCAACAGTC CCGCCTCTGT GGCCTCGTCT 
GGCAGTACCC CTTCCTCTAA AAACAAGGAG CACGGCCATA ATGACAAATC TTCCACGCCT 
GGTCTCAAGT CTAACACCCC TACACCTCGT AACGATGCCC CCACACCAGG TACCAGCACC 
ACCCCTGGGC TGCGGCCCAT CCTGGGCAAG CCTCCGATGG AAGCTCTTGC CGCCCCTGCT 
CTGCGCACCC CTCTCACTTA CCCAACTCCC TTCGCCATGA TGAGCCACCA TGAGATGAAT 
GGTTCTCTGA CCAACCCTGG AGTCTATGCT GGCCTTCACA TCTCTCCTCA GATGAGCGCG 
GCTGCTGCTG CTGCCTACGG GCGCTCGCCC ATGGCGGGTT TCGAGCACCC TCATATGAGA 
GCTCCCGGCC TCCCTGCCAG CCTCACCTCG ATCTCTGGAG GAAAACCAGC CTACTCTTTC 
CACGTCAGCG CCGATGGACA AATGCAACCT GTTCCCTTCC CTCCAGATGC TCTCATTGGT 
CCAGGGATCC CTCGCCATGC CCGGCAAATC AACACACTCA GTCATGGTGA GGTTGTTTGT 
GCCGTGACTA TCAGCAACCC CACGCGCCAC GTCTACACTG GTGGCAAGGG CTGTGTGAAG 
ATCTGGGACA TCAGTCAGCC TGGAAGCAAG AGTCCGGTTT CTCAACTTGA CTGCCTGAAT 
AGAGACAACT ACATTCGATC CTGTAAGCTG TTGCCTGATG GTCGCACACT CATTGTTGGA 
GGAGAGGCCA GCACTCTGAC CATTTGGGAT CTTGCCTCCC AGACACCCCG AATCAAAGCC 
GAGCTGACCT CCTCTGCGCC AGCCTGCTAC GCGCTGGCCA TCAGCCCAGA TGCCAAGGTG 
TGCTTCTCCT GCTGTAGTGA TGGAAACATC GCTGTCTGGG ACCTTCACAA CCAGACCCTT 
GTCAGGCAAT TCCAAGGCCA CACAGATGGT GCCAGCTGCA TTGACATCTC TCATGATGGC 
ACCAAGCTCT GGACAGGTGG TCTTGATAAC ACAGTCCGCT CCTGGGACCT CAGGGAGGGA 
AGGCAACTCC AACAGCACGA CTTCACATCT CAGATCTTCT CTCTGGGCTA CTGTCCAACG 
GGCGAGTGGC TGGCTGTTGG TATGGAAAGC AGCAACGTTG AGGTCCTCCA CCACACCAAG 
CCTGACAAGT ACCAGCTGCA CCTTCACGAG AGCTGCGTTC TCTCTCTCAA GTTTGCATAC 
TGTGGTAAAT GGTTCGTGAG CACTGGGAAG GACAATCTGT TGAATGCATG GAGGACTCCC 
TATGGCGCCA GCATATTCCA GTCAAAGGAA TCATCCTCGG TCCTGAGCTG TGACATATCA 
GCTGATGACA AGTACATTGT TACGGGCTCT GGAGACAAGA AGGCCACGGT GTATGAAGTG 
ATCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300623.1
CDS	134..2422
Translation	MYPQGRHPAPHQPGQPGFKFTVAESCDRIKDEFQFLQAQYHSLKVEYDKLANEKTEMQRHYVMYYEMSYGLNIEMHKQTEIAKRLNAILAQIMPFLSQEHQQQVAQAVERAKQVTMTELNAIIGVRGLPNLPLTQQQLQAQHLSHAAHGPVALPPHPSGLQPPGIPPVTGSGSGLLALGALGSQPHLPVKDEKNHHDLEHRESTNNSISPSDSLRTTSEKHRGSSDYSLDSKKRKVEDKDSMSRYDSDGDKSDDLVVDVSNEDPATPRGSPAHSPPENGIDKPRPAKKDTPNSPASVASSGSTPSSKNKEHGHNDKSSTPGLKSNTPTPRNDAPTPGTSTTPGLRPILGKPPMEALAAPALRTPLTYPTPFAMMSHHEMNGSLTNPGVYAGLHISPQMSAAAAAAYGRSPMAGFEHPHMRAPGLPASLTSISGGKPAYSFHVSADGQMQPVPFPPDALIGPGIPRHARQINTLSHGEVVCAVTISNPTRHVYTGGKGCVKIWDISQPGSKSPVSQLDCLNRDNYIRSCKLLPDGRTLIVGGEASTLTIWDLASQTPRIKAELTSSAPACYALAISPDAKVCFSCCSDGNIAVWDLHNQTLVRQFQGHTDGASCIDISHDGTKLWTGGLDNTVRSWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMESSNVEVLHHTKPDKYQLHLHESCVLSLKFAYCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISADDKYIVTGSGDKKATVYEVIY

Target ORF information:

RefSeq Version	NM_001313694.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio TLE family member 3, transcriptional corepressor a (tle3a), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313694.1

ORF Insert Sequence:

ATGTATCCAC AGGGCCGGCA TCCGGCGCCC CACCAGCCTG GTCAGCCAGG CTTCAAATTC 
ACAGTGGCGG AGTCCTGTGA CAGGATCAAA GACGAATTTC AGTTCCTGCA AGCCCAATAT 
CACAGTCTCA AAGTGGAGTA CGACAAACTG GCCAATGAGA AGACCGAGAT GCAGCGCCAT 
TATGTCATGT ACTATGAGAT GTCCTATGGG CTGAACATTG AAATGCACAA ACAGACCGAG 
ATTGCCAAAA GGCTCAATGC GATTCTAGCC CAGATTATGC CTTTTCTGTC ACAAGAGCAC 
CAACAGCAAG TAGCACAAGC TGTTGAGCGT GCAAAACAAG TGACAATGAC CGAACTGAAT 
GCTATCATCG GGGTACGTGG ACTTCCCAAT CTGCCTCTCA CCCAGCAGCA GCTCCAAGCC 
CAGCACCTCT CTCACGCTGC CCACGGCCCC GTGGCTTTAC CTCCCCACCC TTCTGGCCTT 
CAGCCACCAG GAATCCCTCC GGTCACCGGC TCTGGCTCAG GCCTGCTGGC TCTAGGGGCC 
CTGGGAAGTC AGCCACACTT GCCGGTGAAG GATGAAAAAA ACCACCATGA CCTGGAGCAC 
CGAGAGAGCA CGAACAACTC CATCTCTCCA TCAGACAGCC TGCGCACAAC CAGTGAGAAG 
CACCGTGGCT CCTCCGATTA CAGCTTAGAC TCCAAAAAAC GCAAAGTGGA GGACAAGGAC 
AGCATGAGTC GATATGACAG TGATGGAGAC AAAAGTGATG ATTTGGTGGT TGATGTTTCC 
AATGAGGACC CTGCCACGCC CAGAGGCAGC CCCGCCCACT CGCCGCCAGA GAATGGTATA 
GATAAGCCCC GGCCAGCAAA AAAGGACACA CCCAACAGTC CCGCCTCTGT GGCCTCGTCT 
GGCAGTACCC CTTCCTCTAA AAACAAGGAG CACGGCCATA ATGACAAATC TTCCACGCCT 
GGTCTCAAGT CTAACACCCC TACACCTCGT AACGATGCCC CCACACCAGG TACCAGCACC 
ACCCCTGGGC TGCGGCCCAT CCTGGGCAAG CCTCCGATGG AAGCTCTTGC CGCCCCTGCT 
CTGCGCACCC CTCTCACTTA CCCAACTCCC TTCGCCATGA TGAGCCACCA TGAGATGAAT 
GGTTCTCTGA CCAACCCTGG AGTCTATGCT GGCCTTCACA TCTCTCCTCA GATGAGCGCG 
GCTGCTGCTG CTGCCTACGG GCGCTCGCCC ATGGCGGGTT TCGAGCACCC TCATATGAGA 
GCTCCCGGCC TCCCTGCCAG CCTCACCTCG ATCTCTGGAG GAAAACCAGC CTACTCTTTC 
CACGTCAGCG CCGATGGACA AATGCAACCT GTTCCCTTCC CTCCAGATGC TCTCATTGGT 
CCAGGGATCC CTCGCCATGC CCGGCAAATC AACACACTCA GTCATGGTGA GGTTGTTTGT 
GCCGTGACTA TCAGCAACCC CACGCGCCAC GTCTACACTG GTGGCAAGGG CTGTGTGAAG 
ATCTGGGACA TCAGTCAGCC TGGAAGCAAG AGTCCGGTTT CTCAACTTGA CTGCCTGAAT 
AGAGACAACT ACATTCGATC CTGTAAGCTG TTGCCTGATG GTCGCACACT CATTGTTGGA 
GGAGAGGCCA GCACTCTGAC CATTTGGGAT CTTGCCTCCC AGACACCCCG AATCAAAGCC 
GAGCTGACCT CCTCTGCGCC AGCCTGCTAC GCGCTGGCCA TCAGCCCAGA TGCCAAGGTG 
TGCTTCTCCT GCTGTAGTGA TGGAAACATC GCTGTCTGGG ACCTTCACAA CCAGACCCTT 
GTCAGGCAAT TCCAAGGCCA CACAGATGGT GCCAGCTGCA TTGACATCTC TCATGATGGC 
ACCAAGCTCT GGACAGGTGG TCTTGATAAC ACAGTCCGCT CCTGGGACCT CAGGGAGGGA 
AGGCAACTCC AACAGCACGA CTTCACATCT CAGATCTTCT CTCTGGGCTA CTGTCCAACG 
GGCGAGTGGC TGGCTGTTGG TATGGAAAGC AGCAACGTTG AGGTCCTCCA CCACACCAAG 
CCTGACAAGT ACCAGCTGCA CCTTCACGAG AGCTGCGTTC TCTCTCTCAA GTTTGCATAC 
TGTGGTAAAT GGTTCGTGAG CACTGGGAAG GACAATCTGT TGAATGCATG GAGGACTCCC 
TATGGCGCCA GCATATTCCA GTCAAAGGAA TCATCCTCGG TCCTGAGCTG TGACATATCA 
GCTGATGACA AGTACATTGT TACGGGCTCT GGAGACAAGA AGGCCACGGT GTATGAAGTG 
ATCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tle3a ( NM_131012.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571087.2 Danio rerio TLE family member 3, transcriptional corepressor a (tle3a), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ODa23209
Clone ID Related Accession (Same CDS sequence)	XM_005159041.3 , XM_005159041.4 , NM_131012.2
Accession Version	NM_131012.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-07
Organism	Danio rerio(zebrafish)
Product	transducin-like enhancer protein 3-A isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT613405.2, BX324138.6 and EH525475.1. On Aug 27, 2015 this sequence version replaced NM_131012.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in its 5' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U96340.1, Y12466.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTATCCAC AGGGCCGGCA TCCGGCGCCC CACCAGCCTG GTCAGCCAGG CTTCAAATTC 
ACAGTGGCGG AGTCCTGTGA CAGGATCAAA GACGAATTTC AGTTCCTGCA AGCCCAATAT 
CACAGTCTCA AAGTGGAGTA CGACAAACTG GCCAATGAGA AGACCGAGAT GCAGCGCCAT 
TATGTCATGT ACTATGAGAT GTCCTATGGG CTGAACATTG AAATGCACAA ACAGACCGAG 
ATTGCCAAAA GGCTCAATGC GATTCTAGCC CAGATTATGC CTTTTCTGTC ACAAGAGCAC 
CAACAGCAAG TAGCACAAGC TGTTGAGCGT GCAAAACAAG TGACAATGAC CGAACTGAAT 
GCTATCATCG GGGTACGTGG ACTTCCCAAT CTGCCTCTCA CCCAGCAGCT CCAAGCCCAG 
CACCTCTCTC ACGCTGCCCA CGGCCCCGTG GCTTTACCTC CCCACCCTTC TGGCCTTCAG 
CCACCAGGAA TCCCTCCGGT CACCGGCTCT GGCTCAGGCC TGCTGGCTCT AGGGGCCCTG 
GGAAGTCAGC CACACTTGCC GGTGAAGGAT GAAAAAAACC ACCATGACCT GGAGCACCGA 
GAGAGCACGA ACAACTCCAT CTCTCCATCA GACAGCCTGC GCACAACCAG TGAGAAGCAC 
CGTGGCTCCT CCGATTACAG CTTAGACTCC AAAAAACGCA AAGTGGAGGA CAAGGACAGC 
ATGAGTCGAT ATGACAGTGA TGGAGACAAA AGTGATGATT TGGTGGTTGA TGTTTCCAAT 
GAGGACCCTG CCACGCCCAG AGGCAGCCCC GCCCACTCGC CGCCAGAGAA TGGTATAGAT 
AAGCCCCGGC CAGCAAAAAA GGACACACCC AACAGTCCCG CCTCTGTGGC CTCGTCTGGC 
AGTACCCCTT CCTCTAAAAA CAAGGAGCAC GGCCATAATG ACAAATCTTC CACGCCTGGT 
CTCAAGTCTA ACACCCCTAC ACCTCGTAAC GATGCCCCCA CACCAGGTAC CAGCACCACC 
CCTGGGCTGC GGCCCATCCT GGGCAAGCCT CCGATGGAAG CTCTTGCCGC CCCTGCTCTG 
CGCACCCCTC TCACTTACCC AACTCCCTTC GCCATGATGA GCCACCATGA GATGAATGGT 
TCTCTGACCA ACCCTGGAGT CTATGCTGGC CTTCACATCT CTCCTCAGAT GAGCGCGGCT 
GCTGCTGCTG CCTACGGGCG CTCGCCCATG GCGGGTTTCG AGCACCCTCA TATGAGAGCT 
CCCGGCCTCC CTGCCAGCCT CACCTCGATC TCTGGAGGAA AACCAGCCTA CTCTTTCCAC 
GTCAGCGCCG ATGGACAAAT GCAACCTGTT CCCTTCCCTC CAGATGCTCT CATTGGTCCA 
GGGATCCCTC GCCATGCCCG GCAAATCAAC ACACTCAGTC ATGGTGAGGT TGTTTGTGCC 
GTGACTATCA GCAACCCCAC GCGCCACGTC TACACTGGTG GCAAGGGCTG TGTGAAGATC 
TGGGACATCA GTCAGCCTGG AAGCAAGAGT CCGGTTTCTC AACTTGACTG CCTGAATAGA 
GACAACTACA TTCGATCCTG TAAGCTGTTG CCTGATGGTC GCACACTCAT TGTTGGAGGA 
GAGGCCAGCA CTCTGACCAT TTGGGATCTT GCCTCCCAGA CACCCCGAAT CAAAGCCGAG 
CTGACCTCCT CTGCGCCAGC CTGCTACGCG CTGGCCATCA GCCCAGATGC CAAGGTGTGC 
TTCTCCTGCT GTAGTGATGG AAACATCGCT GTCTGGGACC TTCACAACCA GACCCTTGTC 
AGGCAATTCC AAGGCCACAC AGATGGTGCC AGCTGCATTG ACATCTCTCA TGATGGCACC 
AAGCTCTGGA CAGGTGGTCT TGATAACACA GTCCGCTCCT GGGACCTCAG GGAGGGAAGG 
CAACTCCAAC AGCACGACTT CACATCTCAG ATCTTCTCTC TGGGCTACTG TCCAACGGGC 
GAGTGGCTGG CTGTTGGTAT GGAAAGCAGC AACGTTGAGG TCCTCCACCA CACCAAGCCT 
GACAAGTACC AGCTGCACCT TCACGAGAGC TGCGTTCTCT CTCTCAAGTT TGCATACTGT 
GGTAAATGGT TCGTGAGCAC TGGGAAGGAC AATCTGTTGA ATGCATGGAG GACTCCCTAT 
GGCGCCAGCA TATTCCAGTC AAAGGAATCA TCCTCGGTCC TGAGCTGTGA CATATCAGCT 
GATGACAAGT ACATTGTTAC GGGCTCTGGA GACAAGAAGG CCACGGTGTA TGAAGTGATC 
TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571087.2
CDS	134..2419
Translation	MYPQGRHPAPHQPGQPGFKFTVAESCDRIKDEFQFLQAQYHSLKVEYDKLANEKTEMQRHYVMYYEMSYGLNIEMHKQTEIAKRLNAILAQIMPFLSQEHQQQVAQAVERAKQVTMTELNAIIGVRGLPNLPLTQQLQAQHLSHAAHGPVALPPHPSGLQPPGIPPVTGSGSGLLALGALGSQPHLPVKDEKNHHDLEHRESTNNSISPSDSLRTTSEKHRGSSDYSLDSKKRKVEDKDSMSRYDSDGDKSDDLVVDVSNEDPATPRGSPAHSPPENGIDKPRPAKKDTPNSPASVASSGSTPSSKNKEHGHNDKSSTPGLKSNTPTPRNDAPTPGTSTTPGLRPILGKPPMEALAAPALRTPLTYPTPFAMMSHHEMNGSLTNPGVYAGLHISPQMSAAAAAAYGRSPMAGFEHPHMRAPGLPASLTSISGGKPAYSFHVSADGQMQPVPFPPDALIGPGIPRHARQINTLSHGEVVCAVTISNPTRHVYTGGKGCVKIWDISQPGSKSPVSQLDCLNRDNYIRSCKLLPDGRTLIVGGEASTLTIWDLASQTPRIKAELTSSAPACYALAISPDAKVCFSCCSDGNIAVWDLHNQTLVRQFQGHTDGASCIDISHDGTKLWTGGLDNTVRSWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMESSNVEVLHHTKPDKYQLHLHESCVLSLKFAYCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISADDKYIVTGSGDKKATVYEVIY

Target ORF information:

RefSeq Version	NM_131012.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio TLE family member 3, transcriptional corepressor a (tle3a), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131012.2

ORF Insert Sequence:

ATGTATCCAC AGGGCCGGCA TCCGGCGCCC CACCAGCCTG GTCAGCCAGG CTTCAAATTC 
ACAGTGGCGG AGTCCTGTGA CAGGATCAAA GACGAATTTC AGTTCCTGCA AGCCCAATAT 
CACAGTCTCA AAGTGGAGTA CGACAAACTG GCCAATGAGA AGACCGAGAT GCAGCGCCAT 
TATGTCATGT ACTATGAGAT GTCCTATGGG CTGAACATTG AAATGCACAA ACAGACCGAG 
ATTGCCAAAA GGCTCAATGC GATTCTAGCC CAGATTATGC CTTTTCTGTC ACAAGAGCAC 
CAACAGCAAG TAGCACAAGC TGTTGAGCGT GCAAAACAAG TGACAATGAC CGAACTGAAT 
GCTATCATCG GGGTACGTGG ACTTCCCAAT CTGCCTCTCA CCCAGCAGCT CCAAGCCCAG 
CACCTCTCTC ACGCTGCCCA CGGCCCCGTG GCTTTACCTC CCCACCCTTC TGGCCTTCAG 
CCACCAGGAA TCCCTCCGGT CACCGGCTCT GGCTCAGGCC TGCTGGCTCT AGGGGCCCTG 
GGAAGTCAGC CACACTTGCC GGTGAAGGAT GAAAAAAACC ACCATGACCT GGAGCACCGA 
GAGAGCACGA ACAACTCCAT CTCTCCATCA GACAGCCTGC GCACAACCAG TGAGAAGCAC 
CGTGGCTCCT CCGATTACAG CTTAGACTCC AAAAAACGCA AAGTGGAGGA CAAGGACAGC 
ATGAGTCGAT ATGACAGTGA TGGAGACAAA AGTGATGATT TGGTGGTTGA TGTTTCCAAT 
GAGGACCCTG CCACGCCCAG AGGCAGCCCC GCCCACTCGC CGCCAGAGAA TGGTATAGAT 
AAGCCCCGGC CAGCAAAAAA GGACACACCC AACAGTCCCG CCTCTGTGGC CTCGTCTGGC 
AGTACCCCTT CCTCTAAAAA CAAGGAGCAC GGCCATAATG ACAAATCTTC CACGCCTGGT 
CTCAAGTCTA ACACCCCTAC ACCTCGTAAC GATGCCCCCA CACCAGGTAC CAGCACCACC 
CCTGGGCTGC GGCCCATCCT GGGCAAGCCT CCGATGGAAG CTCTTGCCGC CCCTGCTCTG 
CGCACCCCTC TCACTTACCC AACTCCCTTC GCCATGATGA GCCACCATGA GATGAATGGT 
TCTCTGACCA ACCCTGGAGT CTATGCTGGC CTTCACATCT CTCCTCAGAT GAGCGCGGCT 
GCTGCTGCTG CCTACGGGCG CTCGCCCATG GCGGGTTTCG AGCACCCTCA TATGAGAGCT 
CCCGGCCTCC CTGCCAGCCT CACCTCGATC TCTGGAGGAA AACCAGCCTA CTCTTTCCAC 
GTCAGCGCCG ATGGACAAAT GCAACCTGTT CCCTTCCCTC CAGATGCTCT CATTGGTCCA 
GGGATCCCTC GCCATGCCCG GCAAATCAAC ACACTCAGTC ATGGTGAGGT TGTTTGTGCC 
GTGACTATCA GCAACCCCAC GCGCCACGTC TACACTGGTG GCAAGGGCTG TGTGAAGATC 
TGGGACATCA GTCAGCCTGG AAGCAAGAGT CCGGTTTCTC AACTTGACTG CCTGAATAGA 
GACAACTACA TTCGATCCTG TAAGCTGTTG CCTGATGGTC GCACACTCAT TGTTGGAGGA 
GAGGCCAGCA CTCTGACCAT TTGGGATCTT GCCTCCCAGA CACCCCGAAT CAAAGCCGAG 
CTGACCTCCT CTGCGCCAGC CTGCTACGCG CTGGCCATCA GCCCAGATGC CAAGGTGTGC 
TTCTCCTGCT GTAGTGATGG AAACATCGCT GTCTGGGACC TTCACAACCA GACCCTTGTC 
AGGCAATTCC AAGGCCACAC AGATGGTGCC AGCTGCATTG ACATCTCTCA TGATGGCACC 
AAGCTCTGGA CAGGTGGTCT TGATAACACA GTCCGCTCCT GGGACCTCAG GGAGGGAAGG 
CAACTCCAAC AGCACGACTT CACATCTCAG ATCTTCTCTC TGGGCTACTG TCCAACGGGC 
GAGTGGCTGG CTGTTGGTAT GGAAAGCAGC AACGTTGAGG TCCTCCACCA CACCAAGCCT 
GACAAGTACC AGCTGCACCT TCACGAGAGC TGCGTTCTCT CTCTCAAGTT TGCATACTGT 
GGTAAATGGT TCGTGAGCAC TGGGAAGGAC AATCTGTTGA ATGCATGGAG GACTCCCTAT 
GGCGCCAGCA TATTCCAGTC AAAGGAATCA TCCTCGGTCC TGAGCTGTGA CATATCAGCT 
GATGACAAGT ACATTGTTAC GGGCTCTGGA GACAAGAAGG CCACGGTGTA TGAAGTGATC 
TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tle3a ( XM_005159039.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159096.1 Danio rerio transducin-like enhancer of split 3a (tle3a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa23208
Clone ID Related Accession (Same CDS sequence)	XM_005159039.3 , XM_017351898.1 , XM_017351898.2 , NM_001313694.1 , XM_005159039.4
Accession Version	XM_005159039.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein groucho-2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159039.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATCCAC AGGGCCGGCA TCCGGCGCCC CACCAGCCTG GTCAGCCAGG CTTCAAATTC 
ACAGTGGCGG AGTCCTGTGA CAGGATCAAA GACGAATTTC AGTTCCTGCA AGCCCAATAT 
CACAGTCTCA AAGTGGAGTA CGACAAACTG GCCAATGAGA AGACCGAGAT GCAGCGCCAT 
TATGTCATGT ACTATGAGAT GTCCTATGGG CTGAACATTG AAATGCACAA ACAGACCGAG 
ATTGCCAAAA GGCTCAATGC GATTCTAGCC CAGATTATGC CTTTTCTGTC ACAAGAGCAC 
CAACAGCAAG TAGCACAAGC TGTTGAGCGT GCAAAACAAG TGACAATGAC CGAACTGAAT 
GCTATCATCG GGGTACGTGG ACTTCCCAAT CTGCCTCTCA CCCAGCAGCA GCTCCAAGCC 
CAGCACCTCT CTCACGCTGC CCACGGCCCC GTGGCTTTAC CTCCCCACCC TTCTGGCCTT 
CAGCCACCAG GAATCCCTCC GGTCACCGGC TCTGGCTCAG GCCTGCTGGC TCTAGGGGCC 
CTGGGAAGTC AGCCACACTT GCCGGTGAAG GATGAAAAAA ACCACCATGA CCTGGAGCAC 
CGAGAGAGCA CGAACAACTC CATCTCTCCA TCAGACAGCC TGCGCACAAC CAGTGAGAAG 
CACCGTGGCT CCTCCGATTA CAGCTTAGAC TCCAAAAAAC GCAAAGTGGA GGACAAGGAC 
AGCATGAGTC GATATGACAG TGATGGAGAC AAAAGTGATG ATTTGGTGGT TGATGTTTCC 
AATGAGGACC CTGCCACGCC CAGAGGCAGC CCCGCCCACT CGCCGCCAGA GAATGGTATA 
GATAAGCCCC GGCCAGCAAA AAAGGACACA CCCAACAGTC CCGCCTCTGT GGCCTCGTCT 
GGCAGTACCC CTTCCTCTAA AAACAAGGAG CACGGCCATA ATGACAAATC TTCCACGCCT 
GGTCTCAAGT CTAACACCCC TACACCTCGT AACGATGCCC CCACACCAGG TACCAGCACC 
ACCCCTGGGC TGCGGCCCAT CCTGGGCAAG CCTCCGATGG AAGCTCTTGC CGCCCCTGCT 
CTGCGCACCC CTCTCACTTA CCCAACTCCC TTCGCCATGA TGAGCCACCA TGAGATGAAT 
GGTTCTCTGA CCAACCCTGG AGTCTATGCT GGCCTTCACA TCTCTCCTCA GATGAGCGCG 
GCTGCTGCTG CTGCCTACGG GCGCTCGCCC ATGGCGGGTT TCGAGCACCC TCATATGAGA 
GCTCCCGGCC TCCCTGCCAG CCTCACCTCG ATCTCTGGAG GAAAACCAGC CTACTCTTTC 
CACGTCAGCG CCGATGGACA AATGCAACCT GTTCCCTTCC CTCCAGATGC TCTCATTGGT 
CCAGGGATCC CTCGCCATGC CCGGCAAATC AACACACTCA GTCATGGTGA GGTTGTTTGT 
GCCGTGACTA TCAGCAACCC CACGCGCCAC GTCTACACTG GTGGCAAGGG CTGTGTGAAG 
ATCTGGGACA TCAGTCAGCC TGGAAGCAAG AGTCCGGTTT CTCAACTTGA CTGCCTGAAT 
AGAGACAACT ACATTCGATC CTGTAAGCTG TTGCCTGATG GTCGCACACT CATTGTTGGA 
GGAGAGGCCA GCACTCTGAC CATTTGGGAT CTTGCCTCCC AGACACCCCG AATCAAAGCC 
GAGCTGACCT CCTCTGCGCC AGCCTGCTAC GCGCTGGCCA TCAGCCCAGA TGCCAAGGTG 
TGCTTCTCCT GCTGTAGTGA TGGAAACATC GCTGTCTGGG ACCTTCACAA CCAGACCCTT 
GTCAGGCAAT TCCAAGGCCA CACAGATGGT GCCAGCTGCA TTGACATCTC TCATGATGGC 
ACCAAGCTCT GGACAGGTGG TCTTGATAAC ACAGTCCGCT CCTGGGACCT CAGGGAGGGA 
AGGCAACTCC AACAGCACGA CTTCACATCT CAGATCTTCT CTCTGGGCTA CTGTCCAACG 
GGCGAGTGGC TGGCTGTTGG TATGGAAAGC AGCAACGTTG AGGTCCTCCA CCACACCAAG 
CCTGACAAGT ACCAGCTGCA CCTTCACGAG AGCTGCGTTC TCTCTCTCAA GTTTGCATAC 
TGTGGTAAAT GGTTCGTGAG CACTGGGAAG GACAATCTGT TGAATGCATG GAGGACTCCC 
TATGGCGCCA GCATATTCCA GTCAAAGGAA TCATCCTCGG TCCTGAGCTG TGACATATCA 
GCTGATGACA AGTACATTGT TACGGGCTCT GGAGACAAGA AGGCCACGGT GTATGAAGTG 
ATCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159096.1
CDS	518..2806
Translation	MYPQGRHPAPHQPGQPGFKFTVAESCDRIKDEFQFLQAQYHSLKVEYDKLANEKTEMQRHYVMYYEMSYGLNIEMHKQTEIAKRLNAILAQIMPFLSQEHQQQVAQAVERAKQVTMTELNAIIGVRGLPNLPLTQQQLQAQHLSHAAHGPVALPPHPSGLQPPGIPPVTGSGSGLLALGALGSQPHLPVKDEKNHHDLEHRESTNNSISPSDSLRTTSEKHRGSSDYSLDSKKRKVEDKDSMSRYDSDGDKSDDLVVDVSNEDPATPRGSPAHSPPENGIDKPRPAKKDTPNSPASVASSGSTPSSKNKEHGHNDKSSTPGLKSNTPTPRNDAPTPGTSTTPGLRPILGKPPMEALAAPALRTPLTYPTPFAMMSHHEMNGSLTNPGVYAGLHISPQMSAAAAAAYGRSPMAGFEHPHMRAPGLPASLTSISGGKPAYSFHVSADGQMQPVPFPPDALIGPGIPRHARQINTLSHGEVVCAVTISNPTRHVYTGGKGCVKIWDISQPGSKSPVSQLDCLNRDNYIRSCKLLPDGRTLIVGGEASTLTIWDLASQTPRIKAELTSSAPACYALAISPDAKVCFSCCSDGNIAVWDLHNQTLVRQFQGHTDGASCIDISHDGTKLWTGGLDNTVRSWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMESSNVEVLHHTKPDKYQLHLHESCVLSLKFAYCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISADDKYIVTGSGDKKATVYEVIY

Target ORF information:

RefSeq Version	XM_005159039.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio transducin-like enhancer of split 3a (tle3a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159039.3

ORF Insert Sequence:

ATGTATCCAC AGGGCCGGCA TCCGGCGCCC CACCAGCCTG GTCAGCCAGG CTTCAAATTC 
ACAGTGGCGG AGTCCTGTGA CAGGATCAAA GACGAATTTC AGTTCCTGCA AGCCCAATAT 
CACAGTCTCA AAGTGGAGTA CGACAAACTG GCCAATGAGA AGACCGAGAT GCAGCGCCAT 
TATGTCATGT ACTATGAGAT GTCCTATGGG CTGAACATTG AAATGCACAA ACAGACCGAG 
ATTGCCAAAA GGCTCAATGC GATTCTAGCC CAGATTATGC CTTTTCTGTC ACAAGAGCAC 
CAACAGCAAG TAGCACAAGC TGTTGAGCGT GCAAAACAAG TGACAATGAC CGAACTGAAT 
GCTATCATCG GGGTACGTGG ACTTCCCAAT CTGCCTCTCA CCCAGCAGCA GCTCCAAGCC 
CAGCACCTCT CTCACGCTGC CCACGGCCCC GTGGCTTTAC CTCCCCACCC TTCTGGCCTT 
CAGCCACCAG GAATCCCTCC GGTCACCGGC TCTGGCTCAG GCCTGCTGGC TCTAGGGGCC 
CTGGGAAGTC AGCCACACTT GCCGGTGAAG GATGAAAAAA ACCACCATGA CCTGGAGCAC 
CGAGAGAGCA CGAACAACTC CATCTCTCCA TCAGACAGCC TGCGCACAAC CAGTGAGAAG 
CACCGTGGCT CCTCCGATTA CAGCTTAGAC TCCAAAAAAC GCAAAGTGGA GGACAAGGAC 
AGCATGAGTC GATATGACAG TGATGGAGAC AAAAGTGATG ATTTGGTGGT TGATGTTTCC 
AATGAGGACC CTGCCACGCC CAGAGGCAGC CCCGCCCACT CGCCGCCAGA GAATGGTATA 
GATAAGCCCC GGCCAGCAAA AAAGGACACA CCCAACAGTC CCGCCTCTGT GGCCTCGTCT 
GGCAGTACCC CTTCCTCTAA AAACAAGGAG CACGGCCATA ATGACAAATC TTCCACGCCT 
GGTCTCAAGT CTAACACCCC TACACCTCGT AACGATGCCC CCACACCAGG TACCAGCACC 
ACCCCTGGGC TGCGGCCCAT CCTGGGCAAG CCTCCGATGG AAGCTCTTGC CGCCCCTGCT 
CTGCGCACCC CTCTCACTTA CCCAACTCCC TTCGCCATGA TGAGCCACCA TGAGATGAAT 
GGTTCTCTGA CCAACCCTGG AGTCTATGCT GGCCTTCACA TCTCTCCTCA GATGAGCGCG 
GCTGCTGCTG CTGCCTACGG GCGCTCGCCC ATGGCGGGTT TCGAGCACCC TCATATGAGA 
GCTCCCGGCC TCCCTGCCAG CCTCACCTCG ATCTCTGGAG GAAAACCAGC CTACTCTTTC 
CACGTCAGCG CCGATGGACA AATGCAACCT GTTCCCTTCC CTCCAGATGC TCTCATTGGT 
CCAGGGATCC CTCGCCATGC CCGGCAAATC AACACACTCA GTCATGGTGA GGTTGTTTGT 
GCCGTGACTA TCAGCAACCC CACGCGCCAC GTCTACACTG GTGGCAAGGG CTGTGTGAAG 
ATCTGGGACA TCAGTCAGCC TGGAAGCAAG AGTCCGGTTT CTCAACTTGA CTGCCTGAAT 
AGAGACAACT ACATTCGATC CTGTAAGCTG TTGCCTGATG GTCGCACACT CATTGTTGGA 
GGAGAGGCCA GCACTCTGAC CATTTGGGAT CTTGCCTCCC AGACACCCCG AATCAAAGCC 
GAGCTGACCT CCTCTGCGCC AGCCTGCTAC GCGCTGGCCA TCAGCCCAGA TGCCAAGGTG 
TGCTTCTCCT GCTGTAGTGA TGGAAACATC GCTGTCTGGG ACCTTCACAA CCAGACCCTT 
GTCAGGCAAT TCCAAGGCCA CACAGATGGT GCCAGCTGCA TTGACATCTC TCATGATGGC 
ACCAAGCTCT GGACAGGTGG TCTTGATAAC ACAGTCCGCT CCTGGGACCT CAGGGAGGGA 
AGGCAACTCC AACAGCACGA CTTCACATCT CAGATCTTCT CTCTGGGCTA CTGTCCAACG 
GGCGAGTGGC TGGCTGTTGG TATGGAAAGC AGCAACGTTG AGGTCCTCCA CCACACCAAG 
CCTGACAAGT ACCAGCTGCA CCTTCACGAG AGCTGCGTTC TCTCTCTCAA GTTTGCATAC 
TGTGGTAAAT GGTTCGTGAG CACTGGGAAG GACAATCTGT TGAATGCATG GAGGACTCCC 
TATGGCGCCA GCATATTCCA GTCAAAGGAA TCATCCTCGG TCCTGAGCTG TGACATATCA 
GCTGATGACA AGTACATTGT TACGGGCTCT GGAGACAAGA AGGCCACGGT GTATGAAGTG 
ATCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tle3a ( XM_017351898.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207387.1 Danio rerio transducin-like enhancer of split 3a (tle3a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa23208
Clone ID Related Accession (Same CDS sequence)	XM_005159039.3 , XM_017351898.1 , XM_017351898.2 , NM_001313694.1 , XM_005159039.4
Accession Version	XM_017351898.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein groucho-2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATCCAC AGGGCCGGCA TCCGGCGCCC CACCAGCCTG GTCAGCCAGG CTTCAAATTC 
ACAGTGGCGG AGTCCTGTGA CAGGATCAAA GACGAATTTC AGTTCCTGCA AGCCCAATAT 
CACAGTCTCA AAGTGGAGTA CGACAAACTG GCCAATGAGA AGACCGAGAT GCAGCGCCAT 
TATGTCATGT ACTATGAGAT GTCCTATGGG CTGAACATTG AAATGCACAA ACAGACCGAG 
ATTGCCAAAA GGCTCAATGC GATTCTAGCC CAGATTATGC CTTTTCTGTC ACAAGAGCAC 
CAACAGCAAG TAGCACAAGC TGTTGAGCGT GCAAAACAAG TGACAATGAC CGAACTGAAT 
GCTATCATCG GGGTACGTGG ACTTCCCAAT CTGCCTCTCA CCCAGCAGCA GCTCCAAGCC 
CAGCACCTCT CTCACGCTGC CCACGGCCCC GTGGCTTTAC CTCCCCACCC TTCTGGCCTT 
CAGCCACCAG GAATCCCTCC GGTCACCGGC TCTGGCTCAG GCCTGCTGGC TCTAGGGGCC 
CTGGGAAGTC AGCCACACTT GCCGGTGAAG GATGAAAAAA ACCACCATGA CCTGGAGCAC 
CGAGAGAGCA CGAACAACTC CATCTCTCCA TCAGACAGCC TGCGCACAAC CAGTGAGAAG 
CACCGTGGCT CCTCCGATTA CAGCTTAGAC TCCAAAAAAC GCAAAGTGGA GGACAAGGAC 
AGCATGAGTC GATATGACAG TGATGGAGAC AAAAGTGATG ATTTGGTGGT TGATGTTTCC 
AATGAGGACC CTGCCACGCC CAGAGGCAGC CCCGCCCACT CGCCGCCAGA GAATGGTATA 
GATAAGCCCC GGCCAGCAAA AAAGGACACA CCCAACAGTC CCGCCTCTGT GGCCTCGTCT 
GGCAGTACCC CTTCCTCTAA AAACAAGGAG CACGGCCATA ATGACAAATC TTCCACGCCT 
GGTCTCAAGT CTAACACCCC TACACCTCGT AACGATGCCC CCACACCAGG TACCAGCACC 
ACCCCTGGGC TGCGGCCCAT CCTGGGCAAG CCTCCGATGG AAGCTCTTGC CGCCCCTGCT 
CTGCGCACCC CTCTCACTTA CCCAACTCCC TTCGCCATGA TGAGCCACCA TGAGATGAAT 
GGTTCTCTGA CCAACCCTGG AGTCTATGCT GGCCTTCACA TCTCTCCTCA GATGAGCGCG 
GCTGCTGCTG CTGCCTACGG GCGCTCGCCC ATGGCGGGTT TCGAGCACCC TCATATGAGA 
GCTCCCGGCC TCCCTGCCAG CCTCACCTCG ATCTCTGGAG GAAAACCAGC CTACTCTTTC 
CACGTCAGCG CCGATGGACA AATGCAACCT GTTCCCTTCC CTCCAGATGC TCTCATTGGT 
CCAGGGATCC CTCGCCATGC CCGGCAAATC AACACACTCA GTCATGGTGA GGTTGTTTGT 
GCCGTGACTA TCAGCAACCC CACGCGCCAC GTCTACACTG GTGGCAAGGG CTGTGTGAAG 
ATCTGGGACA TCAGTCAGCC TGGAAGCAAG AGTCCGGTTT CTCAACTTGA CTGCCTGAAT 
AGAGACAACT ACATTCGATC CTGTAAGCTG TTGCCTGATG GTCGCACACT CATTGTTGGA 
GGAGAGGCCA GCACTCTGAC CATTTGGGAT CTTGCCTCCC AGACACCCCG AATCAAAGCC 
GAGCTGACCT CCTCTGCGCC AGCCTGCTAC GCGCTGGCCA TCAGCCCAGA TGCCAAGGTG 
TGCTTCTCCT GCTGTAGTGA TGGAAACATC GCTGTCTGGG ACCTTCACAA CCAGACCCTT 
GTCAGGCAAT TCCAAGGCCA CACAGATGGT GCCAGCTGCA TTGACATCTC TCATGATGGC 
ACCAAGCTCT GGACAGGTGG TCTTGATAAC ACAGTCCGCT CCTGGGACCT CAGGGAGGGA 
AGGCAACTCC AACAGCACGA CTTCACATCT CAGATCTTCT CTCTGGGCTA CTGTCCAACG 
GGCGAGTGGC TGGCTGTTGG TATGGAAAGC AGCAACGTTG AGGTCCTCCA CCACACCAAG 
CCTGACAAGT ACCAGCTGCA CCTTCACGAG AGCTGCGTTC TCTCTCTCAA GTTTGCATAC 
TGTGGTAAAT GGTTCGTGAG CACTGGGAAG GACAATCTGT TGAATGCATG GAGGACTCCC 
TATGGCGCCA GCATATTCCA GTCAAAGGAA TCATCCTCGG TCCTGAGCTG TGACATATCA 
GCTGATGACA AGTACATTGT TACGGGCTCT GGAGACAAGA AGGCCACGGT GTATGAAGTG 
ATCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207387.1
CDS	515..2803
Translation	MYPQGRHPAPHQPGQPGFKFTVAESCDRIKDEFQFLQAQYHSLKVEYDKLANEKTEMQRHYVMYYEMSYGLNIEMHKQTEIAKRLNAILAQIMPFLSQEHQQQVAQAVERAKQVTMTELNAIIGVRGLPNLPLTQQQLQAQHLSHAAHGPVALPPHPSGLQPPGIPPVTGSGSGLLALGALGSQPHLPVKDEKNHHDLEHRESTNNSISPSDSLRTTSEKHRGSSDYSLDSKKRKVEDKDSMSRYDSDGDKSDDLVVDVSNEDPATPRGSPAHSPPENGIDKPRPAKKDTPNSPASVASSGSTPSSKNKEHGHNDKSSTPGLKSNTPTPRNDAPTPGTSTTPGLRPILGKPPMEALAAPALRTPLTYPTPFAMMSHHEMNGSLTNPGVYAGLHISPQMSAAAAAAYGRSPMAGFEHPHMRAPGLPASLTSISGGKPAYSFHVSADGQMQPVPFPPDALIGPGIPRHARQINTLSHGEVVCAVTISNPTRHVYTGGKGCVKIWDISQPGSKSPVSQLDCLNRDNYIRSCKLLPDGRTLIVGGEASTLTIWDLASQTPRIKAELTSSAPACYALAISPDAKVCFSCCSDGNIAVWDLHNQTLVRQFQGHTDGASCIDISHDGTKLWTGGLDNTVRSWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMESSNVEVLHHTKPDKYQLHLHESCVLSLKFAYCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISADDKYIVTGSGDKKATVYEVIY

Target ORF information:

RefSeq Version	XM_017351898.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio transducin-like enhancer of split 3a (tle3a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351898.1

ORF Insert Sequence:

ATGTATCCAC AGGGCCGGCA TCCGGCGCCC CACCAGCCTG GTCAGCCAGG CTTCAAATTC 
ACAGTGGCGG AGTCCTGTGA CAGGATCAAA GACGAATTTC AGTTCCTGCA AGCCCAATAT 
CACAGTCTCA AAGTGGAGTA CGACAAACTG GCCAATGAGA AGACCGAGAT GCAGCGCCAT 
TATGTCATGT ACTATGAGAT GTCCTATGGG CTGAACATTG AAATGCACAA ACAGACCGAG 
ATTGCCAAAA GGCTCAATGC GATTCTAGCC CAGATTATGC CTTTTCTGTC ACAAGAGCAC 
CAACAGCAAG TAGCACAAGC TGTTGAGCGT GCAAAACAAG TGACAATGAC CGAACTGAAT 
GCTATCATCG GGGTACGTGG ACTTCCCAAT CTGCCTCTCA CCCAGCAGCA GCTCCAAGCC 
CAGCACCTCT CTCACGCTGC CCACGGCCCC GTGGCTTTAC CTCCCCACCC TTCTGGCCTT 
CAGCCACCAG GAATCCCTCC GGTCACCGGC TCTGGCTCAG GCCTGCTGGC TCTAGGGGCC 
CTGGGAAGTC AGCCACACTT GCCGGTGAAG GATGAAAAAA ACCACCATGA CCTGGAGCAC 
CGAGAGAGCA CGAACAACTC CATCTCTCCA TCAGACAGCC TGCGCACAAC CAGTGAGAAG 
CACCGTGGCT CCTCCGATTA CAGCTTAGAC TCCAAAAAAC GCAAAGTGGA GGACAAGGAC 
AGCATGAGTC GATATGACAG TGATGGAGAC AAAAGTGATG ATTTGGTGGT TGATGTTTCC 
AATGAGGACC CTGCCACGCC CAGAGGCAGC CCCGCCCACT CGCCGCCAGA GAATGGTATA 
GATAAGCCCC GGCCAGCAAA AAAGGACACA CCCAACAGTC CCGCCTCTGT GGCCTCGTCT 
GGCAGTACCC CTTCCTCTAA AAACAAGGAG CACGGCCATA ATGACAAATC TTCCACGCCT 
GGTCTCAAGT CTAACACCCC TACACCTCGT AACGATGCCC CCACACCAGG TACCAGCACC 
ACCCCTGGGC TGCGGCCCAT CCTGGGCAAG CCTCCGATGG AAGCTCTTGC CGCCCCTGCT 
CTGCGCACCC CTCTCACTTA CCCAACTCCC TTCGCCATGA TGAGCCACCA TGAGATGAAT 
GGTTCTCTGA CCAACCCTGG AGTCTATGCT GGCCTTCACA TCTCTCCTCA GATGAGCGCG 
GCTGCTGCTG CTGCCTACGG GCGCTCGCCC ATGGCGGGTT TCGAGCACCC TCATATGAGA 
GCTCCCGGCC TCCCTGCCAG CCTCACCTCG ATCTCTGGAG GAAAACCAGC CTACTCTTTC 
CACGTCAGCG CCGATGGACA AATGCAACCT GTTCCCTTCC CTCCAGATGC TCTCATTGGT 
CCAGGGATCC CTCGCCATGC CCGGCAAATC AACACACTCA GTCATGGTGA GGTTGTTTGT 
GCCGTGACTA TCAGCAACCC CACGCGCCAC GTCTACACTG GTGGCAAGGG CTGTGTGAAG 
ATCTGGGACA TCAGTCAGCC TGGAAGCAAG AGTCCGGTTT CTCAACTTGA CTGCCTGAAT 
AGAGACAACT ACATTCGATC CTGTAAGCTG TTGCCTGATG GTCGCACACT CATTGTTGGA 
GGAGAGGCCA GCACTCTGAC CATTTGGGAT CTTGCCTCCC AGACACCCCG AATCAAAGCC 
GAGCTGACCT CCTCTGCGCC AGCCTGCTAC GCGCTGGCCA TCAGCCCAGA TGCCAAGGTG 
TGCTTCTCCT GCTGTAGTGA TGGAAACATC GCTGTCTGGG ACCTTCACAA CCAGACCCTT 
GTCAGGCAAT TCCAAGGCCA CACAGATGGT GCCAGCTGCA TTGACATCTC TCATGATGGC 
ACCAAGCTCT GGACAGGTGG TCTTGATAAC ACAGTCCGCT CCTGGGACCT CAGGGAGGGA 
AGGCAACTCC AACAGCACGA CTTCACATCT CAGATCTTCT CTCTGGGCTA CTGTCCAACG 
GGCGAGTGGC TGGCTGTTGG TATGGAAAGC AGCAACGTTG AGGTCCTCCA CCACACCAAG 
CCTGACAAGT ACCAGCTGCA CCTTCACGAG AGCTGCGTTC TCTCTCTCAA GTTTGCATAC 
TGTGGTAAAT GGTTCGTGAG CACTGGGAAG GACAATCTGT TGAATGCATG GAGGACTCCC 
TATGGCGCCA GCATATTCCA GTCAAAGGAA TCATCCTCGG TCCTGAGCTG TGACATATCA 
GCTGATGACA AGTACATTGT TACGGGCTCT GGAGACAAGA AGGCCACGGT GTATGAAGTG 
ATCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tle3a ( XM_017351898.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207387.1 Danio rerio transducin-like enhancer of split 3a (tle3a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa23208
Clone ID Related Accession (Same CDS sequence)	XM_005159039.3 , XM_017351898.1 , XM_017351898.2 , NM_001313694.1 , XM_005159039.4
Accession Version	XM_017351898.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein groucho-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017351898.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATCCAC AGGGCCGGCA TCCGGCGCCC CACCAGCCTG GTCAGCCAGG CTTCAAATTC 
ACAGTGGCGG AGTCCTGTGA CAGGATCAAA GACGAATTTC AGTTCCTGCA AGCCCAATAT 
CACAGTCTCA AAGTGGAGTA CGACAAACTG GCCAATGAGA AGACCGAGAT GCAGCGCCAT 
TATGTCATGT ACTATGAGAT GTCCTATGGG CTGAACATTG AAATGCACAA ACAGACCGAG 
ATTGCCAAAA GGCTCAATGC GATTCTAGCC CAGATTATGC CTTTTCTGTC ACAAGAGCAC 
CAACAGCAAG TAGCACAAGC TGTTGAGCGT GCAAAACAAG TGACAATGAC CGAACTGAAT 
GCTATCATCG GGGTACGTGG ACTTCCCAAT CTGCCTCTCA CCCAGCAGCA GCTCCAAGCC 
CAGCACCTCT CTCACGCTGC CCACGGCCCC GTGGCTTTAC CTCCCCACCC TTCTGGCCTT 
CAGCCACCAG GAATCCCTCC GGTCACCGGC TCTGGCTCAG GCCTGCTGGC TCTAGGGGCC 
CTGGGAAGTC AGCCACACTT GCCGGTGAAG GATGAAAAAA ACCACCATGA CCTGGAGCAC 
CGAGAGAGCA CGAACAACTC CATCTCTCCA TCAGACAGCC TGCGCACAAC CAGTGAGAAG 
CACCGTGGCT CCTCCGATTA CAGCTTAGAC TCCAAAAAAC GCAAAGTGGA GGACAAGGAC 
AGCATGAGTC GATATGACAG TGATGGAGAC AAAAGTGATG ATTTGGTGGT TGATGTTTCC 
AATGAGGACC CTGCCACGCC CAGAGGCAGC CCCGCCCACT CGCCGCCAGA GAATGGTATA 
GATAAGCCCC GGCCAGCAAA AAAGGACACA CCCAACAGTC CCGCCTCTGT GGCCTCGTCT 
GGCAGTACCC CTTCCTCTAA AAACAAGGAG CACGGCCATA ATGACAAATC TTCCACGCCT 
GGTCTCAAGT CTAACACCCC TACACCTCGT AACGATGCCC CCACACCAGG TACCAGCACC 
ACCCCTGGGC TGCGGCCCAT CCTGGGCAAG CCTCCGATGG AAGCTCTTGC CGCCCCTGCT 
CTGCGCACCC CTCTCACTTA CCCAACTCCC TTCGCCATGA TGAGCCACCA TGAGATGAAT 
GGTTCTCTGA CCAACCCTGG AGTCTATGCT GGCCTTCACA TCTCTCCTCA GATGAGCGCG 
GCTGCTGCTG CTGCCTACGG GCGCTCGCCC ATGGCGGGTT TCGAGCACCC TCATATGAGA 
GCTCCCGGCC TCCCTGCCAG CCTCACCTCG ATCTCTGGAG GAAAACCAGC CTACTCTTTC 
CACGTCAGCG CCGATGGACA AATGCAACCT GTTCCCTTCC CTCCAGATGC TCTCATTGGT 
CCAGGGATCC CTCGCCATGC CCGGCAAATC AACACACTCA GTCATGGTGA GGTTGTTTGT 
GCCGTGACTA TCAGCAACCC CACGCGCCAC GTCTACACTG GTGGCAAGGG CTGTGTGAAG 
ATCTGGGACA TCAGTCAGCC TGGAAGCAAG AGTCCGGTTT CTCAACTTGA CTGCCTGAAT 
AGAGACAACT ACATTCGATC CTGTAAGCTG TTGCCTGATG GTCGCACACT CATTGTTGGA 
GGAGAGGCCA GCACTCTGAC CATTTGGGAT CTTGCCTCCC AGACACCCCG AATCAAAGCC 
GAGCTGACCT CCTCTGCGCC AGCCTGCTAC GCGCTGGCCA TCAGCCCAGA TGCCAAGGTG 
TGCTTCTCCT GCTGTAGTGA TGGAAACATC GCTGTCTGGG ACCTTCACAA CCAGACCCTT 
GTCAGGCAAT TCCAAGGCCA CACAGATGGT GCCAGCTGCA TTGACATCTC TCATGATGGC 
ACCAAGCTCT GGACAGGTGG TCTTGATAAC ACAGTCCGCT CCTGGGACCT CAGGGAGGGA 
AGGCAACTCC AACAGCACGA CTTCACATCT CAGATCTTCT CTCTGGGCTA CTGTCCAACG 
GGCGAGTGGC TGGCTGTTGG TATGGAAAGC AGCAACGTTG AGGTCCTCCA CCACACCAAG 
CCTGACAAGT ACCAGCTGCA CCTTCACGAG AGCTGCGTTC TCTCTCTCAA GTTTGCATAC 
TGTGGTAAAT GGTTCGTGAG CACTGGGAAG GACAATCTGT TGAATGCATG GAGGACTCCC 
TATGGCGCCA GCATATTCCA GTCAAAGGAA TCATCCTCGG TCCTGAGCTG TGACATATCA 
GCTGATGACA AGTACATTGT TACGGGCTCT GGAGACAAGA AGGCCACGGT GTATGAAGTG 
ATCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207387.1
CDS	516..2804
Translation	MYPQGRHPAPHQPGQPGFKFTVAESCDRIKDEFQFLQAQYHSLKVEYDKLANEKTEMQRHYVMYYEMSYGLNIEMHKQTEIAKRLNAILAQIMPFLSQEHQQQVAQAVERAKQVTMTELNAIIGVRGLPNLPLTQQQLQAQHLSHAAHGPVALPPHPSGLQPPGIPPVTGSGSGLLALGALGSQPHLPVKDEKNHHDLEHRESTNNSISPSDSLRTTSEKHRGSSDYSLDSKKRKVEDKDSMSRYDSDGDKSDDLVVDVSNEDPATPRGSPAHSPPENGIDKPRPAKKDTPNSPASVASSGSTPSSKNKEHGHNDKSSTPGLKSNTPTPRNDAPTPGTSTTPGLRPILGKPPMEALAAPALRTPLTYPTPFAMMSHHEMNGSLTNPGVYAGLHISPQMSAAAAAAYGRSPMAGFEHPHMRAPGLPASLTSISGGKPAYSFHVSADGQMQPVPFPPDALIGPGIPRHARQINTLSHGEVVCAVTISNPTRHVYTGGKGCVKIWDISQPGSKSPVSQLDCLNRDNYIRSCKLLPDGRTLIVGGEASTLTIWDLASQTPRIKAELTSSAPACYALAISPDAKVCFSCCSDGNIAVWDLHNQTLVRQFQGHTDGASCIDISHDGTKLWTGGLDNTVRSWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMESSNVEVLHHTKPDKYQLHLHESCVLSLKFAYCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISADDKYIVTGSGDKKATVYEVIY

Target ORF information:

RefSeq Version	XM_017351898.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transducin-like enhancer of split 3a (tle3a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351898.2

ORF Insert Sequence:

ATGTATCCAC AGGGCCGGCA TCCGGCGCCC CACCAGCCTG GTCAGCCAGG CTTCAAATTC 
ACAGTGGCGG AGTCCTGTGA CAGGATCAAA GACGAATTTC AGTTCCTGCA AGCCCAATAT 
CACAGTCTCA AAGTGGAGTA CGACAAACTG GCCAATGAGA AGACCGAGAT GCAGCGCCAT 
TATGTCATGT ACTATGAGAT GTCCTATGGG CTGAACATTG AAATGCACAA ACAGACCGAG 
ATTGCCAAAA GGCTCAATGC GATTCTAGCC CAGATTATGC CTTTTCTGTC ACAAGAGCAC 
CAACAGCAAG TAGCACAAGC TGTTGAGCGT GCAAAACAAG TGACAATGAC CGAACTGAAT 
GCTATCATCG GGGTACGTGG ACTTCCCAAT CTGCCTCTCA CCCAGCAGCA GCTCCAAGCC 
CAGCACCTCT CTCACGCTGC CCACGGCCCC GTGGCTTTAC CTCCCCACCC TTCTGGCCTT 
CAGCCACCAG GAATCCCTCC GGTCACCGGC TCTGGCTCAG GCCTGCTGGC TCTAGGGGCC 
CTGGGAAGTC AGCCACACTT GCCGGTGAAG GATGAAAAAA ACCACCATGA CCTGGAGCAC 
CGAGAGAGCA CGAACAACTC CATCTCTCCA TCAGACAGCC TGCGCACAAC CAGTGAGAAG 
CACCGTGGCT CCTCCGATTA CAGCTTAGAC TCCAAAAAAC GCAAAGTGGA GGACAAGGAC 
AGCATGAGTC GATATGACAG TGATGGAGAC AAAAGTGATG ATTTGGTGGT TGATGTTTCC 
AATGAGGACC CTGCCACGCC CAGAGGCAGC CCCGCCCACT CGCCGCCAGA GAATGGTATA 
GATAAGCCCC GGCCAGCAAA AAAGGACACA CCCAACAGTC CCGCCTCTGT GGCCTCGTCT 
GGCAGTACCC CTTCCTCTAA AAACAAGGAG CACGGCCATA ATGACAAATC TTCCACGCCT 
GGTCTCAAGT CTAACACCCC TACACCTCGT AACGATGCCC CCACACCAGG TACCAGCACC 
ACCCCTGGGC TGCGGCCCAT CCTGGGCAAG CCTCCGATGG AAGCTCTTGC CGCCCCTGCT 
CTGCGCACCC CTCTCACTTA CCCAACTCCC TTCGCCATGA TGAGCCACCA TGAGATGAAT 
GGTTCTCTGA CCAACCCTGG AGTCTATGCT GGCCTTCACA TCTCTCCTCA GATGAGCGCG 
GCTGCTGCTG CTGCCTACGG GCGCTCGCCC ATGGCGGGTT TCGAGCACCC TCATATGAGA 
GCTCCCGGCC TCCCTGCCAG CCTCACCTCG ATCTCTGGAG GAAAACCAGC CTACTCTTTC 
CACGTCAGCG CCGATGGACA AATGCAACCT GTTCCCTTCC CTCCAGATGC TCTCATTGGT 
CCAGGGATCC CTCGCCATGC CCGGCAAATC AACACACTCA GTCATGGTGA GGTTGTTTGT 
GCCGTGACTA TCAGCAACCC CACGCGCCAC GTCTACACTG GTGGCAAGGG CTGTGTGAAG 
ATCTGGGACA TCAGTCAGCC TGGAAGCAAG AGTCCGGTTT CTCAACTTGA CTGCCTGAAT 
AGAGACAACT ACATTCGATC CTGTAAGCTG TTGCCTGATG GTCGCACACT CATTGTTGGA 
GGAGAGGCCA GCACTCTGAC CATTTGGGAT CTTGCCTCCC AGACACCCCG AATCAAAGCC 
GAGCTGACCT CCTCTGCGCC AGCCTGCTAC GCGCTGGCCA TCAGCCCAGA TGCCAAGGTG 
TGCTTCTCCT GCTGTAGTGA TGGAAACATC GCTGTCTGGG ACCTTCACAA CCAGACCCTT 
GTCAGGCAAT TCCAAGGCCA CACAGATGGT GCCAGCTGCA TTGACATCTC TCATGATGGC 
ACCAAGCTCT GGACAGGTGG TCTTGATAAC ACAGTCCGCT CCTGGGACCT CAGGGAGGGA 
AGGCAACTCC AACAGCACGA CTTCACATCT CAGATCTTCT CTCTGGGCTA CTGTCCAACG 
GGCGAGTGGC TGGCTGTTGG TATGGAAAGC AGCAACGTTG AGGTCCTCCA CCACACCAAG 
CCTGACAAGT ACCAGCTGCA CCTTCACGAG AGCTGCGTTC TCTCTCTCAA GTTTGCATAC 
TGTGGTAAAT GGTTCGTGAG CACTGGGAAG GACAATCTGT TGAATGCATG GAGGACTCCC 
TATGGCGCCA GCATATTCCA GTCAAAGGAA TCATCCTCGG TCCTGAGCTG TGACATATCA 
GCTGATGACA AGTACATTGT TACGGGCTCT GGAGACAAGA AGGCCACGGT GTATGAAGTG 
ATCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tle3a ( XM_005159039.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159096.1 Danio rerio transducin-like enhancer of split 3a (tle3a), transcript variant X1, mRNA.

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CloneID	ODa23208
Clone ID Related Accession (Same CDS sequence)	XM_005159039.3 , XM_017351898.1 , XM_017351898.2 , NM_001313694.1 , XM_005159039.4
Accession Version	XM_005159039.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein groucho-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159039.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATCCAC AGGGCCGGCA TCCGGCGCCC CACCAGCCTG GTCAGCCAGG CTTCAAATTC 
ACAGTGGCGG AGTCCTGTGA CAGGATCAAA GACGAATTTC AGTTCCTGCA AGCCCAATAT 
CACAGTCTCA AAGTGGAGTA CGACAAACTG GCCAATGAGA AGACCGAGAT GCAGCGCCAT 
TATGTCATGT ACTATGAGAT GTCCTATGGG CTGAACATTG AAATGCACAA ACAGACCGAG 
ATTGCCAAAA GGCTCAATGC GATTCTAGCC CAGATTATGC CTTTTCTGTC ACAAGAGCAC 
CAACAGCAAG TAGCACAAGC TGTTGAGCGT GCAAAACAAG TGACAATGAC CGAACTGAAT 
GCTATCATCG GGGTACGTGG ACTTCCCAAT CTGCCTCTCA CCCAGCAGCA GCTCCAAGCC 
CAGCACCTCT CTCACGCTGC CCACGGCCCC GTGGCTTTAC CTCCCCACCC TTCTGGCCTT 
CAGCCACCAG GAATCCCTCC GGTCACCGGC TCTGGCTCAG GCCTGCTGGC TCTAGGGGCC 
CTGGGAAGTC AGCCACACTT GCCGGTGAAG GATGAAAAAA ACCACCATGA CCTGGAGCAC 
CGAGAGAGCA CGAACAACTC CATCTCTCCA TCAGACAGCC TGCGCACAAC CAGTGAGAAG 
CACCGTGGCT CCTCCGATTA CAGCTTAGAC TCCAAAAAAC GCAAAGTGGA GGACAAGGAC 
AGCATGAGTC GATATGACAG TGATGGAGAC AAAAGTGATG ATTTGGTGGT TGATGTTTCC 
AATGAGGACC CTGCCACGCC CAGAGGCAGC CCCGCCCACT CGCCGCCAGA GAATGGTATA 
GATAAGCCCC GGCCAGCAAA AAAGGACACA CCCAACAGTC CCGCCTCTGT GGCCTCGTCT 
GGCAGTACCC CTTCCTCTAA AAACAAGGAG CACGGCCATA ATGACAAATC TTCCACGCCT 
GGTCTCAAGT CTAACACCCC TACACCTCGT AACGATGCCC CCACACCAGG TACCAGCACC 
ACCCCTGGGC TGCGGCCCAT CCTGGGCAAG CCTCCGATGG AAGCTCTTGC CGCCCCTGCT 
CTGCGCACCC CTCTCACTTA CCCAACTCCC TTCGCCATGA TGAGCCACCA TGAGATGAAT 
GGTTCTCTGA CCAACCCTGG AGTCTATGCT GGCCTTCACA TCTCTCCTCA GATGAGCGCG 
GCTGCTGCTG CTGCCTACGG GCGCTCGCCC ATGGCGGGTT TCGAGCACCC TCATATGAGA 
GCTCCCGGCC TCCCTGCCAG CCTCACCTCG ATCTCTGGAG GAAAACCAGC CTACTCTTTC 
CACGTCAGCG CCGATGGACA AATGCAACCT GTTCCCTTCC CTCCAGATGC TCTCATTGGT 
CCAGGGATCC CTCGCCATGC CCGGCAAATC AACACACTCA GTCATGGTGA GGTTGTTTGT 
GCCGTGACTA TCAGCAACCC CACGCGCCAC GTCTACACTG GTGGCAAGGG CTGTGTGAAG 
ATCTGGGACA TCAGTCAGCC TGGAAGCAAG AGTCCGGTTT CTCAACTTGA CTGCCTGAAT 
AGAGACAACT ACATTCGATC CTGTAAGCTG TTGCCTGATG GTCGCACACT CATTGTTGGA 
GGAGAGGCCA GCACTCTGAC CATTTGGGAT CTTGCCTCCC AGACACCCCG AATCAAAGCC 
GAGCTGACCT CCTCTGCGCC AGCCTGCTAC GCGCTGGCCA TCAGCCCAGA TGCCAAGGTG 
TGCTTCTCCT GCTGTAGTGA TGGAAACATC GCTGTCTGGG ACCTTCACAA CCAGACCCTT 
GTCAGGCAAT TCCAAGGCCA CACAGATGGT GCCAGCTGCA TTGACATCTC TCATGATGGC 
ACCAAGCTCT GGACAGGTGG TCTTGATAAC ACAGTCCGCT CCTGGGACCT CAGGGAGGGA 
AGGCAACTCC AACAGCACGA CTTCACATCT CAGATCTTCT CTCTGGGCTA CTGTCCAACG 
GGCGAGTGGC TGGCTGTTGG TATGGAAAGC AGCAACGTTG AGGTCCTCCA CCACACCAAG 
CCTGACAAGT ACCAGCTGCA CCTTCACGAG AGCTGCGTTC TCTCTCTCAA GTTTGCATAC 
TGTGGTAAAT GGTTCGTGAG CACTGGGAAG GACAATCTGT TGAATGCATG GAGGACTCCC 
TATGGCGCCA GCATATTCCA GTCAAAGGAA TCATCCTCGG TCCTGAGCTG TGACATATCA 
GCTGATGACA AGTACATTGT TACGGGCTCT GGAGACAAGA AGGCCACGGT GTATGAAGTG 
ATCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159096.1
CDS	519..2807
Translation	MYPQGRHPAPHQPGQPGFKFTVAESCDRIKDEFQFLQAQYHSLKVEYDKLANEKTEMQRHYVMYYEMSYGLNIEMHKQTEIAKRLNAILAQIMPFLSQEHQQQVAQAVERAKQVTMTELNAIIGVRGLPNLPLTQQQLQAQHLSHAAHGPVALPPHPSGLQPPGIPPVTGSGSGLLALGALGSQPHLPVKDEKNHHDLEHRESTNNSISPSDSLRTTSEKHRGSSDYSLDSKKRKVEDKDSMSRYDSDGDKSDDLVVDVSNEDPATPRGSPAHSPPENGIDKPRPAKKDTPNSPASVASSGSTPSSKNKEHGHNDKSSTPGLKSNTPTPRNDAPTPGTSTTPGLRPILGKPPMEALAAPALRTPLTYPTPFAMMSHHEMNGSLTNPGVYAGLHISPQMSAAAAAAYGRSPMAGFEHPHMRAPGLPASLTSISGGKPAYSFHVSADGQMQPVPFPPDALIGPGIPRHARQINTLSHGEVVCAVTISNPTRHVYTGGKGCVKIWDISQPGSKSPVSQLDCLNRDNYIRSCKLLPDGRTLIVGGEASTLTIWDLASQTPRIKAELTSSAPACYALAISPDAKVCFSCCSDGNIAVWDLHNQTLVRQFQGHTDGASCIDISHDGTKLWTGGLDNTVRSWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMESSNVEVLHHTKPDKYQLHLHESCVLSLKFAYCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISADDKYIVTGSGDKKATVYEVIY

Target ORF information:

RefSeq Version	XM_005159039.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transducin-like enhancer of split 3a (tle3a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159039.4

ORF Insert Sequence:

ATGTATCCAC AGGGCCGGCA TCCGGCGCCC CACCAGCCTG GTCAGCCAGG CTTCAAATTC 
ACAGTGGCGG AGTCCTGTGA CAGGATCAAA GACGAATTTC AGTTCCTGCA AGCCCAATAT 
CACAGTCTCA AAGTGGAGTA CGACAAACTG GCCAATGAGA AGACCGAGAT GCAGCGCCAT 
TATGTCATGT ACTATGAGAT GTCCTATGGG CTGAACATTG AAATGCACAA ACAGACCGAG 
ATTGCCAAAA GGCTCAATGC GATTCTAGCC CAGATTATGC CTTTTCTGTC ACAAGAGCAC 
CAACAGCAAG TAGCACAAGC TGTTGAGCGT GCAAAACAAG TGACAATGAC CGAACTGAAT 
GCTATCATCG GGGTACGTGG ACTTCCCAAT CTGCCTCTCA CCCAGCAGCA GCTCCAAGCC 
CAGCACCTCT CTCACGCTGC CCACGGCCCC GTGGCTTTAC CTCCCCACCC TTCTGGCCTT 
CAGCCACCAG GAATCCCTCC GGTCACCGGC TCTGGCTCAG GCCTGCTGGC TCTAGGGGCC 
CTGGGAAGTC AGCCACACTT GCCGGTGAAG GATGAAAAAA ACCACCATGA CCTGGAGCAC 
CGAGAGAGCA CGAACAACTC CATCTCTCCA TCAGACAGCC TGCGCACAAC CAGTGAGAAG 
CACCGTGGCT CCTCCGATTA CAGCTTAGAC TCCAAAAAAC GCAAAGTGGA GGACAAGGAC 
AGCATGAGTC GATATGACAG TGATGGAGAC AAAAGTGATG ATTTGGTGGT TGATGTTTCC 
AATGAGGACC CTGCCACGCC CAGAGGCAGC CCCGCCCACT CGCCGCCAGA GAATGGTATA 
GATAAGCCCC GGCCAGCAAA AAAGGACACA CCCAACAGTC CCGCCTCTGT GGCCTCGTCT 
GGCAGTACCC CTTCCTCTAA AAACAAGGAG CACGGCCATA ATGACAAATC TTCCACGCCT 
GGTCTCAAGT CTAACACCCC TACACCTCGT AACGATGCCC CCACACCAGG TACCAGCACC 
ACCCCTGGGC TGCGGCCCAT CCTGGGCAAG CCTCCGATGG AAGCTCTTGC CGCCCCTGCT 
CTGCGCACCC CTCTCACTTA CCCAACTCCC TTCGCCATGA TGAGCCACCA TGAGATGAAT 
GGTTCTCTGA CCAACCCTGG AGTCTATGCT GGCCTTCACA TCTCTCCTCA GATGAGCGCG 
GCTGCTGCTG CTGCCTACGG GCGCTCGCCC ATGGCGGGTT TCGAGCACCC TCATATGAGA 
GCTCCCGGCC TCCCTGCCAG CCTCACCTCG ATCTCTGGAG GAAAACCAGC CTACTCTTTC 
CACGTCAGCG CCGATGGACA AATGCAACCT GTTCCCTTCC CTCCAGATGC TCTCATTGGT 
CCAGGGATCC CTCGCCATGC CCGGCAAATC AACACACTCA GTCATGGTGA GGTTGTTTGT 
GCCGTGACTA TCAGCAACCC CACGCGCCAC GTCTACACTG GTGGCAAGGG CTGTGTGAAG 
ATCTGGGACA TCAGTCAGCC TGGAAGCAAG AGTCCGGTTT CTCAACTTGA CTGCCTGAAT 
AGAGACAACT ACATTCGATC CTGTAAGCTG TTGCCTGATG GTCGCACACT CATTGTTGGA 
GGAGAGGCCA GCACTCTGAC CATTTGGGAT CTTGCCTCCC AGACACCCCG AATCAAAGCC 
GAGCTGACCT CCTCTGCGCC AGCCTGCTAC GCGCTGGCCA TCAGCCCAGA TGCCAAGGTG 
TGCTTCTCCT GCTGTAGTGA TGGAAACATC GCTGTCTGGG ACCTTCACAA CCAGACCCTT 
GTCAGGCAAT TCCAAGGCCA CACAGATGGT GCCAGCTGCA TTGACATCTC TCATGATGGC 
ACCAAGCTCT GGACAGGTGG TCTTGATAAC ACAGTCCGCT CCTGGGACCT CAGGGAGGGA 
AGGCAACTCC AACAGCACGA CTTCACATCT CAGATCTTCT CTCTGGGCTA CTGTCCAACG 
GGCGAGTGGC TGGCTGTTGG TATGGAAAGC AGCAACGTTG AGGTCCTCCA CCACACCAAG 
CCTGACAAGT ACCAGCTGCA CCTTCACGAG AGCTGCGTTC TCTCTCTCAA GTTTGCATAC 
TGTGGTAAAT GGTTCGTGAG CACTGGGAAG GACAATCTGT TGAATGCATG GAGGACTCCC 
TATGGCGCCA GCATATTCCA GTCAAAGGAA TCATCCTCGG TCCTGAGCTG TGACATATCA 
GCTGATGACA AGTACATTGT TACGGGCTCT GGAGACAAGA AGGCCACGGT GTATGAAGTG 
ATCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tle3a ( XM_005159041.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159098.1 Danio rerio transducin-like enhancer of split 3a (tle3a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa23209
Clone ID Related Accession (Same CDS sequence)	XM_005159041.3 , XM_005159041.4 , NM_131012.2
Accession Version	XM_005159041.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein groucho-2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159041.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATCCAC AGGGCCGGCA TCCGGCGCCC CACCAGCCTG GTCAGCCAGG CTTCAAATTC 
ACAGTGGCGG AGTCCTGTGA CAGGATCAAA GACGAATTTC AGTTCCTGCA AGCCCAATAT 
CACAGTCTCA AAGTGGAGTA CGACAAACTG GCCAATGAGA AGACCGAGAT GCAGCGCCAT 
TATGTCATGT ACTATGAGAT GTCCTATGGG CTGAACATTG AAATGCACAA ACAGACCGAG 
ATTGCCAAAA GGCTCAATGC GATTCTAGCC CAGATTATGC CTTTTCTGTC ACAAGAGCAC 
CAACAGCAAG TAGCACAAGC TGTTGAGCGT GCAAAACAAG TGACAATGAC CGAACTGAAT 
GCTATCATCG GGGTACGTGG ACTTCCCAAT CTGCCTCTCA CCCAGCAGCT CCAAGCCCAG 
CACCTCTCTC ACGCTGCCCA CGGCCCCGTG GCTTTACCTC CCCACCCTTC TGGCCTTCAG 
CCACCAGGAA TCCCTCCGGT CACCGGCTCT GGCTCAGGCC TGCTGGCTCT AGGGGCCCTG 
GGAAGTCAGC CACACTTGCC GGTGAAGGAT GAAAAAAACC ACCATGACCT GGAGCACCGA 
GAGAGCACGA ACAACTCCAT CTCTCCATCA GACAGCCTGC GCACAACCAG TGAGAAGCAC 
CGTGGCTCCT CCGATTACAG CTTAGACTCC AAAAAACGCA AAGTGGAGGA CAAGGACAGC 
ATGAGTCGAT ATGACAGTGA TGGAGACAAA AGTGATGATT TGGTGGTTGA TGTTTCCAAT 
GAGGACCCTG CCACGCCCAG AGGCAGCCCC GCCCACTCGC CGCCAGAGAA TGGTATAGAT 
AAGCCCCGGC CAGCAAAAAA GGACACACCC AACAGTCCCG CCTCTGTGGC CTCGTCTGGC 
AGTACCCCTT CCTCTAAAAA CAAGGAGCAC GGCCATAATG ACAAATCTTC CACGCCTGGT 
CTCAAGTCTA ACACCCCTAC ACCTCGTAAC GATGCCCCCA CACCAGGTAC CAGCACCACC 
CCTGGGCTGC GGCCCATCCT GGGCAAGCCT CCGATGGAAG CTCTTGCCGC CCCTGCTCTG 
CGCACCCCTC TCACTTACCC AACTCCCTTC GCCATGATGA GCCACCATGA GATGAATGGT 
TCTCTGACCA ACCCTGGAGT CTATGCTGGC CTTCACATCT CTCCTCAGAT GAGCGCGGCT 
GCTGCTGCTG CCTACGGGCG CTCGCCCATG GCGGGTTTCG AGCACCCTCA TATGAGAGCT 
CCCGGCCTCC CTGCCAGCCT CACCTCGATC TCTGGAGGAA AACCAGCCTA CTCTTTCCAC 
GTCAGCGCCG ATGGACAAAT GCAACCTGTT CCCTTCCCTC CAGATGCTCT CATTGGTCCA 
GGGATCCCTC GCCATGCCCG GCAAATCAAC ACACTCAGTC ATGGTGAGGT TGTTTGTGCC 
GTGACTATCA GCAACCCCAC GCGCCACGTC TACACTGGTG GCAAGGGCTG TGTGAAGATC 
TGGGACATCA GTCAGCCTGG AAGCAAGAGT CCGGTTTCTC AACTTGACTG CCTGAATAGA 
GACAACTACA TTCGATCCTG TAAGCTGTTG CCTGATGGTC GCACACTCAT TGTTGGAGGA 
GAGGCCAGCA CTCTGACCAT TTGGGATCTT GCCTCCCAGA CACCCCGAAT CAAAGCCGAG 
CTGACCTCCT CTGCGCCAGC CTGCTACGCG CTGGCCATCA GCCCAGATGC CAAGGTGTGC 
TTCTCCTGCT GTAGTGATGG AAACATCGCT GTCTGGGACC TTCACAACCA GACCCTTGTC 
AGGCAATTCC AAGGCCACAC AGATGGTGCC AGCTGCATTG ACATCTCTCA TGATGGCACC 
AAGCTCTGGA CAGGTGGTCT TGATAACACA GTCCGCTCCT GGGACCTCAG GGAGGGAAGG 
CAACTCCAAC AGCACGACTT CACATCTCAG ATCTTCTCTC TGGGCTACTG TCCAACGGGC 
GAGTGGCTGG CTGTTGGTAT GGAAAGCAGC AACGTTGAGG TCCTCCACCA CACCAAGCCT 
GACAAGTACC AGCTGCACCT TCACGAGAGC TGCGTTCTCT CTCTCAAGTT TGCATACTGT 
GGTAAATGGT TCGTGAGCAC TGGGAAGGAC AATCTGTTGA ATGCATGGAG GACTCCCTAT 
GGCGCCAGCA TATTCCAGTC AAAGGAATCA TCCTCGGTCC TGAGCTGTGA CATATCAGCT 
GATGACAAGT ACATTGTTAC GGGCTCTGGA GACAAGAAGG CCACGGTGTA TGAAGTGATC 
TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159098.1
CDS	518..2803
Translation	MYPQGRHPAPHQPGQPGFKFTVAESCDRIKDEFQFLQAQYHSLKVEYDKLANEKTEMQRHYVMYYEMSYGLNIEMHKQTEIAKRLNAILAQIMPFLSQEHQQQVAQAVERAKQVTMTELNAIIGVRGLPNLPLTQQLQAQHLSHAAHGPVALPPHPSGLQPPGIPPVTGSGSGLLALGALGSQPHLPVKDEKNHHDLEHRESTNNSISPSDSLRTTSEKHRGSSDYSLDSKKRKVEDKDSMSRYDSDGDKSDDLVVDVSNEDPATPRGSPAHSPPENGIDKPRPAKKDTPNSPASVASSGSTPSSKNKEHGHNDKSSTPGLKSNTPTPRNDAPTPGTSTTPGLRPILGKPPMEALAAPALRTPLTYPTPFAMMSHHEMNGSLTNPGVYAGLHISPQMSAAAAAAYGRSPMAGFEHPHMRAPGLPASLTSISGGKPAYSFHVSADGQMQPVPFPPDALIGPGIPRHARQINTLSHGEVVCAVTISNPTRHVYTGGKGCVKIWDISQPGSKSPVSQLDCLNRDNYIRSCKLLPDGRTLIVGGEASTLTIWDLASQTPRIKAELTSSAPACYALAISPDAKVCFSCCSDGNIAVWDLHNQTLVRQFQGHTDGASCIDISHDGTKLWTGGLDNTVRSWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMESSNVEVLHHTKPDKYQLHLHESCVLSLKFAYCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISADDKYIVTGSGDKKATVYEVIY

Target ORF information:

RefSeq Version	XM_005159041.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio transducin-like enhancer of split 3a (tle3a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159041.3

ORF Insert Sequence:

ATGTATCCAC AGGGCCGGCA TCCGGCGCCC CACCAGCCTG GTCAGCCAGG CTTCAAATTC 
ACAGTGGCGG AGTCCTGTGA CAGGATCAAA GACGAATTTC AGTTCCTGCA AGCCCAATAT 
CACAGTCTCA AAGTGGAGTA CGACAAACTG GCCAATGAGA AGACCGAGAT GCAGCGCCAT 
TATGTCATGT ACTATGAGAT GTCCTATGGG CTGAACATTG AAATGCACAA ACAGACCGAG 
ATTGCCAAAA GGCTCAATGC GATTCTAGCC CAGATTATGC CTTTTCTGTC ACAAGAGCAC 
CAACAGCAAG TAGCACAAGC TGTTGAGCGT GCAAAACAAG TGACAATGAC CGAACTGAAT 
GCTATCATCG GGGTACGTGG ACTTCCCAAT CTGCCTCTCA CCCAGCAGCT CCAAGCCCAG 
CACCTCTCTC ACGCTGCCCA CGGCCCCGTG GCTTTACCTC CCCACCCTTC TGGCCTTCAG 
CCACCAGGAA TCCCTCCGGT CACCGGCTCT GGCTCAGGCC TGCTGGCTCT AGGGGCCCTG 
GGAAGTCAGC CACACTTGCC GGTGAAGGAT GAAAAAAACC ACCATGACCT GGAGCACCGA 
GAGAGCACGA ACAACTCCAT CTCTCCATCA GACAGCCTGC GCACAACCAG TGAGAAGCAC 
CGTGGCTCCT CCGATTACAG CTTAGACTCC AAAAAACGCA AAGTGGAGGA CAAGGACAGC 
ATGAGTCGAT ATGACAGTGA TGGAGACAAA AGTGATGATT TGGTGGTTGA TGTTTCCAAT 
GAGGACCCTG CCACGCCCAG AGGCAGCCCC GCCCACTCGC CGCCAGAGAA TGGTATAGAT 
AAGCCCCGGC CAGCAAAAAA GGACACACCC AACAGTCCCG CCTCTGTGGC CTCGTCTGGC 
AGTACCCCTT CCTCTAAAAA CAAGGAGCAC GGCCATAATG ACAAATCTTC CACGCCTGGT 
CTCAAGTCTA ACACCCCTAC ACCTCGTAAC GATGCCCCCA CACCAGGTAC CAGCACCACC 
CCTGGGCTGC GGCCCATCCT GGGCAAGCCT CCGATGGAAG CTCTTGCCGC CCCTGCTCTG 
CGCACCCCTC TCACTTACCC AACTCCCTTC GCCATGATGA GCCACCATGA GATGAATGGT 
TCTCTGACCA ACCCTGGAGT CTATGCTGGC CTTCACATCT CTCCTCAGAT GAGCGCGGCT 
GCTGCTGCTG CCTACGGGCG CTCGCCCATG GCGGGTTTCG AGCACCCTCA TATGAGAGCT 
CCCGGCCTCC CTGCCAGCCT CACCTCGATC TCTGGAGGAA AACCAGCCTA CTCTTTCCAC 
GTCAGCGCCG ATGGACAAAT GCAACCTGTT CCCTTCCCTC CAGATGCTCT CATTGGTCCA 
GGGATCCCTC GCCATGCCCG GCAAATCAAC ACACTCAGTC ATGGTGAGGT TGTTTGTGCC 
GTGACTATCA GCAACCCCAC GCGCCACGTC TACACTGGTG GCAAGGGCTG TGTGAAGATC 
TGGGACATCA GTCAGCCTGG AAGCAAGAGT CCGGTTTCTC AACTTGACTG CCTGAATAGA 
GACAACTACA TTCGATCCTG TAAGCTGTTG CCTGATGGTC GCACACTCAT TGTTGGAGGA 
GAGGCCAGCA CTCTGACCAT TTGGGATCTT GCCTCCCAGA CACCCCGAAT CAAAGCCGAG 
CTGACCTCCT CTGCGCCAGC CTGCTACGCG CTGGCCATCA GCCCAGATGC CAAGGTGTGC 
TTCTCCTGCT GTAGTGATGG AAACATCGCT GTCTGGGACC TTCACAACCA GACCCTTGTC 
AGGCAATTCC AAGGCCACAC AGATGGTGCC AGCTGCATTG ACATCTCTCA TGATGGCACC 
AAGCTCTGGA CAGGTGGTCT TGATAACACA GTCCGCTCCT GGGACCTCAG GGAGGGAAGG 
CAACTCCAAC AGCACGACTT CACATCTCAG ATCTTCTCTC TGGGCTACTG TCCAACGGGC 
GAGTGGCTGG CTGTTGGTAT GGAAAGCAGC AACGTTGAGG TCCTCCACCA CACCAAGCCT 
GACAAGTACC AGCTGCACCT TCACGAGAGC TGCGTTCTCT CTCTCAAGTT TGCATACTGT 
GGTAAATGGT TCGTGAGCAC TGGGAAGGAC AATCTGTTGA ATGCATGGAG GACTCCCTAT 
GGCGCCAGCA TATTCCAGTC AAAGGAATCA TCCTCGGTCC TGAGCTGTGA CATATCAGCT 
GATGACAAGT ACATTGTTAC GGGCTCTGGA GACAAGAAGG CCACGGTGTA TGAAGTGATC 
TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tle3a ( XM_005159041.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159098.1 Danio rerio transducin-like enhancer of split 3a (tle3a), transcript variant X3, mRNA.

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CloneID	ODa23209
Clone ID Related Accession (Same CDS sequence)	XM_005159041.3 , XM_005159041.4 , NM_131012.2
Accession Version	XM_005159041.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein groucho-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159041.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATCCAC AGGGCCGGCA TCCGGCGCCC CACCAGCCTG GTCAGCCAGG CTTCAAATTC 
ACAGTGGCGG AGTCCTGTGA CAGGATCAAA GACGAATTTC AGTTCCTGCA AGCCCAATAT 
CACAGTCTCA AAGTGGAGTA CGACAAACTG GCCAATGAGA AGACCGAGAT GCAGCGCCAT 
TATGTCATGT ACTATGAGAT GTCCTATGGG CTGAACATTG AAATGCACAA ACAGACCGAG 
ATTGCCAAAA GGCTCAATGC GATTCTAGCC CAGATTATGC CTTTTCTGTC ACAAGAGCAC 
CAACAGCAAG TAGCACAAGC TGTTGAGCGT GCAAAACAAG TGACAATGAC CGAACTGAAT 
GCTATCATCG GGGTACGTGG ACTTCCCAAT CTGCCTCTCA CCCAGCAGCT CCAAGCCCAG 
CACCTCTCTC ACGCTGCCCA CGGCCCCGTG GCTTTACCTC CCCACCCTTC TGGCCTTCAG 
CCACCAGGAA TCCCTCCGGT CACCGGCTCT GGCTCAGGCC TGCTGGCTCT AGGGGCCCTG 
GGAAGTCAGC CACACTTGCC GGTGAAGGAT GAAAAAAACC ACCATGACCT GGAGCACCGA 
GAGAGCACGA ACAACTCCAT CTCTCCATCA GACAGCCTGC GCACAACCAG TGAGAAGCAC 
CGTGGCTCCT CCGATTACAG CTTAGACTCC AAAAAACGCA AAGTGGAGGA CAAGGACAGC 
ATGAGTCGAT ATGACAGTGA TGGAGACAAA AGTGATGATT TGGTGGTTGA TGTTTCCAAT 
GAGGACCCTG CCACGCCCAG AGGCAGCCCC GCCCACTCGC CGCCAGAGAA TGGTATAGAT 
AAGCCCCGGC CAGCAAAAAA GGACACACCC AACAGTCCCG CCTCTGTGGC CTCGTCTGGC 
AGTACCCCTT CCTCTAAAAA CAAGGAGCAC GGCCATAATG ACAAATCTTC CACGCCTGGT 
CTCAAGTCTA ACACCCCTAC ACCTCGTAAC GATGCCCCCA CACCAGGTAC CAGCACCACC 
CCTGGGCTGC GGCCCATCCT GGGCAAGCCT CCGATGGAAG CTCTTGCCGC CCCTGCTCTG 
CGCACCCCTC TCACTTACCC AACTCCCTTC GCCATGATGA GCCACCATGA GATGAATGGT 
TCTCTGACCA ACCCTGGAGT CTATGCTGGC CTTCACATCT CTCCTCAGAT GAGCGCGGCT 
GCTGCTGCTG CCTACGGGCG CTCGCCCATG GCGGGTTTCG AGCACCCTCA TATGAGAGCT 
CCCGGCCTCC CTGCCAGCCT CACCTCGATC TCTGGAGGAA AACCAGCCTA CTCTTTCCAC 
GTCAGCGCCG ATGGACAAAT GCAACCTGTT CCCTTCCCTC CAGATGCTCT CATTGGTCCA 
GGGATCCCTC GCCATGCCCG GCAAATCAAC ACACTCAGTC ATGGTGAGGT TGTTTGTGCC 
GTGACTATCA GCAACCCCAC GCGCCACGTC TACACTGGTG GCAAGGGCTG TGTGAAGATC 
TGGGACATCA GTCAGCCTGG AAGCAAGAGT CCGGTTTCTC AACTTGACTG CCTGAATAGA 
GACAACTACA TTCGATCCTG TAAGCTGTTG CCTGATGGTC GCACACTCAT TGTTGGAGGA 
GAGGCCAGCA CTCTGACCAT TTGGGATCTT GCCTCCCAGA CACCCCGAAT CAAAGCCGAG 
CTGACCTCCT CTGCGCCAGC CTGCTACGCG CTGGCCATCA GCCCAGATGC CAAGGTGTGC 
TTCTCCTGCT GTAGTGATGG AAACATCGCT GTCTGGGACC TTCACAACCA GACCCTTGTC 
AGGCAATTCC AAGGCCACAC AGATGGTGCC AGCTGCATTG ACATCTCTCA TGATGGCACC 
AAGCTCTGGA CAGGTGGTCT TGATAACACA GTCCGCTCCT GGGACCTCAG GGAGGGAAGG 
CAACTCCAAC AGCACGACTT CACATCTCAG ATCTTCTCTC TGGGCTACTG TCCAACGGGC 
GAGTGGCTGG CTGTTGGTAT GGAAAGCAGC AACGTTGAGG TCCTCCACCA CACCAAGCCT 
GACAAGTACC AGCTGCACCT TCACGAGAGC TGCGTTCTCT CTCTCAAGTT TGCATACTGT 
GGTAAATGGT TCGTGAGCAC TGGGAAGGAC AATCTGTTGA ATGCATGGAG GACTCCCTAT 
GGCGCCAGCA TATTCCAGTC AAAGGAATCA TCCTCGGTCC TGAGCTGTGA CATATCAGCT 
GATGACAAGT ACATTGTTAC GGGCTCTGGA GACAAGAAGG CCACGGTGTA TGAAGTGATC 
TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159098.1
CDS	519..2804
Translation	MYPQGRHPAPHQPGQPGFKFTVAESCDRIKDEFQFLQAQYHSLKVEYDKLANEKTEMQRHYVMYYEMSYGLNIEMHKQTEIAKRLNAILAQIMPFLSQEHQQQVAQAVERAKQVTMTELNAIIGVRGLPNLPLTQQLQAQHLSHAAHGPVALPPHPSGLQPPGIPPVTGSGSGLLALGALGSQPHLPVKDEKNHHDLEHRESTNNSISPSDSLRTTSEKHRGSSDYSLDSKKRKVEDKDSMSRYDSDGDKSDDLVVDVSNEDPATPRGSPAHSPPENGIDKPRPAKKDTPNSPASVASSGSTPSSKNKEHGHNDKSSTPGLKSNTPTPRNDAPTPGTSTTPGLRPILGKPPMEALAAPALRTPLTYPTPFAMMSHHEMNGSLTNPGVYAGLHISPQMSAAAAAAYGRSPMAGFEHPHMRAPGLPASLTSISGGKPAYSFHVSADGQMQPVPFPPDALIGPGIPRHARQINTLSHGEVVCAVTISNPTRHVYTGGKGCVKIWDISQPGSKSPVSQLDCLNRDNYIRSCKLLPDGRTLIVGGEASTLTIWDLASQTPRIKAELTSSAPACYALAISPDAKVCFSCCSDGNIAVWDLHNQTLVRQFQGHTDGASCIDISHDGTKLWTGGLDNTVRSWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMESSNVEVLHHTKPDKYQLHLHESCVLSLKFAYCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISADDKYIVTGSGDKKATVYEVIY

Target ORF information:

RefSeq Version	XM_005159041.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transducin-like enhancer of split 3a (tle3a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159041.4

ORF Insert Sequence:

ATGTATCCAC AGGGCCGGCA TCCGGCGCCC CACCAGCCTG GTCAGCCAGG CTTCAAATTC 
ACAGTGGCGG AGTCCTGTGA CAGGATCAAA GACGAATTTC AGTTCCTGCA AGCCCAATAT 
CACAGTCTCA AAGTGGAGTA CGACAAACTG GCCAATGAGA AGACCGAGAT GCAGCGCCAT 
TATGTCATGT ACTATGAGAT GTCCTATGGG CTGAACATTG AAATGCACAA ACAGACCGAG 
ATTGCCAAAA GGCTCAATGC GATTCTAGCC CAGATTATGC CTTTTCTGTC ACAAGAGCAC 
CAACAGCAAG TAGCACAAGC TGTTGAGCGT GCAAAACAAG TGACAATGAC CGAACTGAAT 
GCTATCATCG GGGTACGTGG ACTTCCCAAT CTGCCTCTCA CCCAGCAGCT CCAAGCCCAG 
CACCTCTCTC ACGCTGCCCA CGGCCCCGTG GCTTTACCTC CCCACCCTTC TGGCCTTCAG 
CCACCAGGAA TCCCTCCGGT CACCGGCTCT GGCTCAGGCC TGCTGGCTCT AGGGGCCCTG 
GGAAGTCAGC CACACTTGCC GGTGAAGGAT GAAAAAAACC ACCATGACCT GGAGCACCGA 
GAGAGCACGA ACAACTCCAT CTCTCCATCA GACAGCCTGC GCACAACCAG TGAGAAGCAC 
CGTGGCTCCT CCGATTACAG CTTAGACTCC AAAAAACGCA AAGTGGAGGA CAAGGACAGC 
ATGAGTCGAT ATGACAGTGA TGGAGACAAA AGTGATGATT TGGTGGTTGA TGTTTCCAAT 
GAGGACCCTG CCACGCCCAG AGGCAGCCCC GCCCACTCGC CGCCAGAGAA TGGTATAGAT 
AAGCCCCGGC CAGCAAAAAA GGACACACCC AACAGTCCCG CCTCTGTGGC CTCGTCTGGC 
AGTACCCCTT CCTCTAAAAA CAAGGAGCAC GGCCATAATG ACAAATCTTC CACGCCTGGT 
CTCAAGTCTA ACACCCCTAC ACCTCGTAAC GATGCCCCCA CACCAGGTAC CAGCACCACC 
CCTGGGCTGC GGCCCATCCT GGGCAAGCCT CCGATGGAAG CTCTTGCCGC CCCTGCTCTG 
CGCACCCCTC TCACTTACCC AACTCCCTTC GCCATGATGA GCCACCATGA GATGAATGGT 
TCTCTGACCA ACCCTGGAGT CTATGCTGGC CTTCACATCT CTCCTCAGAT GAGCGCGGCT 
GCTGCTGCTG CCTACGGGCG CTCGCCCATG GCGGGTTTCG AGCACCCTCA TATGAGAGCT 
CCCGGCCTCC CTGCCAGCCT CACCTCGATC TCTGGAGGAA AACCAGCCTA CTCTTTCCAC 
GTCAGCGCCG ATGGACAAAT GCAACCTGTT CCCTTCCCTC CAGATGCTCT CATTGGTCCA 
GGGATCCCTC GCCATGCCCG GCAAATCAAC ACACTCAGTC ATGGTGAGGT TGTTTGTGCC 
GTGACTATCA GCAACCCCAC GCGCCACGTC TACACTGGTG GCAAGGGCTG TGTGAAGATC 
TGGGACATCA GTCAGCCTGG AAGCAAGAGT CCGGTTTCTC AACTTGACTG CCTGAATAGA 
GACAACTACA TTCGATCCTG TAAGCTGTTG CCTGATGGTC GCACACTCAT TGTTGGAGGA 
GAGGCCAGCA CTCTGACCAT TTGGGATCTT GCCTCCCAGA CACCCCGAAT CAAAGCCGAG 
CTGACCTCCT CTGCGCCAGC CTGCTACGCG CTGGCCATCA GCCCAGATGC CAAGGTGTGC 
TTCTCCTGCT GTAGTGATGG AAACATCGCT GTCTGGGACC TTCACAACCA GACCCTTGTC 
AGGCAATTCC AAGGCCACAC AGATGGTGCC AGCTGCATTG ACATCTCTCA TGATGGCACC 
AAGCTCTGGA CAGGTGGTCT TGATAACACA GTCCGCTCCT GGGACCTCAG GGAGGGAAGG 
CAACTCCAAC AGCACGACTT CACATCTCAG ATCTTCTCTC TGGGCTACTG TCCAACGGGC 
GAGTGGCTGG CTGTTGGTAT GGAAAGCAGC AACGTTGAGG TCCTCCACCA CACCAAGCCT 
GACAAGTACC AGCTGCACCT TCACGAGAGC TGCGTTCTCT CTCTCAAGTT TGCATACTGT 
GGTAAATGGT TCGTGAGCAC TGGGAAGGAC AATCTGTTGA ATGCATGGAG GACTCCCTAT 
GGCGCCAGCA TATTCCAGTC AAAGGAATCA TCCTCGGTCC TGAGCTGTGA CATATCAGCT 
GATGACAAGT ACATTGTTAC GGGCTCTGGA GACAAGAAGG CCACGGTGTA TGAAGTGATC 
TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transcriptional and histological alterations in gonad of adult zebrafish after exposure to the synthetic progestin norgestrel.
Environmental toxicology and chemistry36(12)3267-3276(2017 Dec)
Shi WJ,Zhao JL,Jiang YX,Huang GY,Liu YS,Zhang JN,Ying GG

An Integrative Developmental Genomics and Systems Biology Approach to Identify an In Vivo Sox Trio-Mediated Gene Regulatory Network in Murine Embryos.
BioMed research international20178932583(2017)
Lee WJ,Chatterjee S,Yap SP,Lim SL,Xing X,Kraus P,Sun W,Hu X,Sivakamasundari V,Chan HY,Kolatkar PR,Prabhakar S,Lufkin T

Testing multiple hypotheses through IMP weighted FDR based on a genetic functional network with application to a new zebrafish transcriptome study.
BioData mining817(2015)
Gui J,Greene CS,Sullivan C,Taylor W,Moore JH,Kim C

Two zebrafish homologues of the Drosophila neurogenic gene groucho and their pattern of transcription during early embryogenesis.
Development genes and evolution207(3)156-166(1997 Aug)
W?lbeck C,Campos-Ortega JA

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tle3a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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