trpm7 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	trpm7
Entrez Gene ID	280653
Full Name	transient receptor potential cation channel, subfamily M, member 7
Synonyms	cb495,tct
General protein information	Preferred Names transient receptor potential cation channel, subfamily M, member 7 Names transient receptor potential cation channel subfamily M member 7 nutria touchtone transient receptor potential melastatin 7
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001030061.1	NP_001025232.1	transient receptor potential cation channel subfamily M member 7

Pathways from BioSystems

Homologies

Homo sapiens (human)	TRPM7	NP_060142.3
Pan troglodytes (chimpanzee)	TRPM7	XP_510400.2
Gallus gallus (chicken)	TRPM7	NP_001171026.1
Danio rerio (zebrafish)	trpm7	NP_001025232.1
Canis lupus familiaris (dog)	TRPM7	XP_005638461.1
Mus musculus (house mouse)	Trpm7	NP_001157797.1
Rattus norvegicus (Norway rat)	Trpm7	NP_446157.2
Xenopus tropicalis (tropical clawed frog)	trpm7	XP_002939674.2
Bos taurus (cattle)	TRPM7	NP_001193095.1

Gene Ontology
Bibliography

Related articles in PubMed

The tbx2a/b transcription factors direct pronephros segmentation and corpuscle of Stannius formation in zebrafish.
Drummond BE, Li Y, Marra AN, Cheng CN, Wingert RA
Developmental biology421(1)52-66(2017 Jan)

Prostaglandin signaling regulates nephron segment patterning of renal progenitors during zebrafish kidney development.
Poureetezadi SJ, Cheng CN, Chambers JM, Drummond BE, Wingert RA
eLife5(2016 12)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Abnormal differentiation of dopaminergic neurons in zebrafish trpm7 mutant larvae impairs development of the motor pattern.
Title: Abnormal differentiation of dopaminergic neurons in zebrafish trpm7 mutant larvae impairs development of the motor pattern.

TRPM7 plays a role within sensory neurons in modulating neutrotransmitter release at central synapses, similar to the role proprosed for mammalian TRPM7 at peripheral synapses.
Title: TRPM7 is required within zebrafish sensory neurons for the activation of touch-evoked escape behaviors.

Trpm7 regulates exocrine pancreatic development via the Mg(2+)-sensitive Socs3a pathway.
Title: Transient receptor potential ion channel Trpm7 regulates exocrine pancreatic epithelial proliferation by Mg2+-sensitive Socs3a signaling in development and cancer.

This study showed that stanniocalcin 1 (stc1)modulates cation levels in trpm7 mutants and in the wild type; levels of cations are restored to normal in trpm7 mutants when stc1 activity is blocked.
Title: trpm7 regulation of in vivo cation homeostasis and kidney function involves stanniocalcin 1 and fgf23.

Cell death of melanophores in zebrafish trpm7 mutant embryos depends on melanin synthesis.
Title: Cell death of melanophores in zebrafish trpm7 mutant embryos depends on melanin synthesis.

The following trpm7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the trpm7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa00854	NM_001030061.1 Latest version!	Danio rerio transient receptor potential cation channel, subfamily M, member 7 (trpm7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

trpm7 ( NM_001030061.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001025232.1 Danio rerio transient receptor potential cation channel, subfamily M, member 7 (trpm7), mRNA.

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CloneID	ODa00854
Clone ID Related Accession (Same CDS sequence)	NM_001030061.1
Accession Version	NM_001030061.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Danio rerio(zebrafish)
Product	transient receptor potential cation channel subfamily M member 7
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY860421.1. On Aug 6, 2005 this sequence version replaced XM_693614.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY860421.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTCCCAGA AGTCCTGGAT CGAACACACT TTCACCAGGA GGGAATGTGT CTATATTCTG 
CCCGTGTCCA AGGATCCGCA CAGATGCCTC CCAGGATGCC AGATCTGCCA GCAGCTCGTG 
CGGTGCTGCT GTGGGCGTCT GGTGCGTCAG CATGTGGGCT TCACGGCCAC ACTGGCGGTC 
AAGTACTCGG AGGTGTCGGA GGAGCTGTCG GAGCTGGAGC AGTGGAGCGT GGAGAAACAC 
ACAGAGGAGA GTCCCACTGA TGCGTACGGG GTCATCAACT TCCAGGGCGG ATCACACTCG 
TACAGGGCCA AGTATGTGCG TCTGTCACAT GACTCGCGTC CGGAGGCGGT GCTGCGGCTG 
ATGCTGAAGG AGTGGCTGAT GGAGCTGCCG AAGATCGTCA TCAGCGTTCA CGGAGGCATC 
CAGAACTTCG AGCTGCACCC GCGCATCAAA CAGGTGGTGG GGAAGGGCCT GATCAAAGCT 
GCTGTCACCA CTGGAGCCTG GATCCTGACC GGAGGGGTCA ACACAGGTGT GGCCAAACAT 
GTGGGTGATG CGCTGAAGGA ACATCTGTCC AGATCGGCTC GGAAAATCTG TACGGTGGGA 
ATCGCACCCT GGGGAGTGAT CGAAAACAGG AACGACCTCA TCGGACGAGA CGTTGTAGCA 
CCGTATCAAA CGCTGCTGAA TCCTCTCAGT AAACTCAACG TCCTCAATAA CTTCCACTCG 
CACTTCCTGC TGGTGGACGA CGGCACTGTG GGCAAATATG GAGCTGAAGT CAGACTGAGG 
AGAGAGCTGG AGAAACACAT CCAACTGCAG AGGATACACG CACGCATTGG TCAGGGTGTT 
CCAGTGGTGG CGTTGATCTT TGAAGGGGGG CCGAATGTGA TCCTGACGGT GCTGGAGTAT 
CTGCAGGAGA GCCCGCCGGT GCCCGTGGTG GTGTGTGAGG GCACGGGACG CGCTGCGGAC 
ATACTGGCAT ACGTGCACAA GCAGACTGAG CAAGATGGTG CGCTGCCTGA CGGTGTGGAG 
TCCGACATCA TTGCAACAAT CAAGAAAACC TTCAACTTCA GTCACGGCGA CGCGCTGCAC 
CTGTTCCAGA CACTGATGGA GTGTATGAAG AACAAAGAGC TGATCACTGT GTTTCCTGTG 
GGCTCTGAGG ATCATCAGGA CATTGATGTA GCGATTCTGA AGGCTCTCCT CAAAGGCACA 
AATGCGACAG CGTTCGATCA GCTGGTGTTG ACGCTGGCGT GGGATCGCGT GGACATCGCT 
AAAGATCACG TGTTTGTTTA CGGACAGCAG CTGCTGGTGG GTTCTCTAGA GCAGGCTATG 
CTGGATGCAT TAGTGATGGA CCGTGTGGAG TTCGTCAAAC TGCTGATAGA GAACGGCGTC 
AGCATGCACA GATTCCTGAC CATCACACGC CTGGAGGAGC TCTACAACAC GAAACAAACA 
CCAACAAACC CGACGCTGTA TCACCTGGTC AGAGACGTCA AACAGGGGAA TCTCCCGCCC 
AACTATAAGA TCACACTGAT TGACGTTGGT CTGGTGATCG AGTATCTGAT GGGAGGAACG 
TACAGATGCA ACTACACACG CAAACGCTTC CGCATCATCT ACAACAACCT GCACGGGAAC 
AGCAGACGGT CGGGGCGACA CACCAGCAGC TCTCACTCTC ATGAGTCCTT CAGCATTCAG 
GCAGATAAGA AGGAGAAAAC CAGACACAAC CACTTCATCA AGACCGCACA GCCGTACAAG 
CCCAGGCTGG ACTCGGTGTC AGAGCTGCAG AAGAAGAAGA GTAAGGAGGA GGTGGTGGAC 
GTGGACGACC CGGAGACGCG GCGCTTCCCG TACCCGTTCA ACGAGCTGCT GGTGTGGGCG 
GTGCTGATGA AGCGGCAGAA GATGAGTCTG TTCTTCTGGC AGCACGGGGA GGAGTCCATG 
GCCAAAGCGC TGGTGGCCTG CAGACTGCTG CGCTCGCTCG GGGACGAGGC CAAGAAGAGC 
GACGTGGTGG ACGACACCTC CGAGGAGCTC AAGGAGTACT CCAGTGAGTT CGGCACGCTG 
GCGGTGGACC TGCTGGAGCA GTCGTTCCGT CAGGACGAGA CGATGGCGAT GAAGCTGTTG 
ACGTACGAGC TGAAGAACTG GAGCAACTCC ACCTGTCTGA AGCTGGCGGT GTCGTCACGG 
TTACGGCCCT TCGTGGCACA CACCTGCACA CAGATGCTGC TGTCCGACAT GTGGATGGGC 
AGACTCAACA TGCGCAAGAA CTCCTGGTAC AAGGTGATCT TGAGTATCCT GGTTCCTCCG 
GCCATCCTCC TGCTGGAGTA CAAGACCAAA GCGGAAATGT CCCACATTCC CCAGAGTCAG 
GACGCACACC AGAGCATGGA GGACAGCGAA CACAACCTGC AGCACCCAGA CGACATACAG 
ATGGATGTGT TTAAGGAAGT GCGTATGAGT GATAATGCAG AAATGAAGAG TGAGTTGGAG 
GTGCACGTGC GCTCCCGCAG GTTACCCATC ACACGCAAGT TCTACGCCTT CTACCACGCG 
CCCATCGTCA AGTTCTGGTT CAACACGCTG TTCTACATCG GTTTCCTGAT GCTCTACTCA 
TTCGTGGTTC TGGTGAAGAT GCAGGATCTT CCGTCTCCTC AGGAGTGGGT CGTCATTCTC 
TACATCTTCA CACTCGCGGT GGAGAAGATA CGAGAGATGT TCATGTCTGA GGGAGGAAAG 
ATCAGTCAGA AGATTAAGGT CTGGTTCAGC GATTACTTCA ACATCTCTGA TTTCCTGGCC 
ATCCTGATGT TCTTCGTGGG CTTCGGTCTG CGTCTGGTGT CGGAGGTGTT CATCGCGGGC 
CGCATCGTGT ACTGCCTGAA CATCATCTTC TGGTACGTGC GGCTGCTGGA CATCCTGGCG 
GTCAACCAGC AGGCCGGCCC GTACGTCATG ATGATCGGCA AGATGGTGGC CAACATGTTC 
TACATCGTGG TCATCATGGC GGTGGTGCTG CTGAGCTTCG GTGTTCCCCG TAAAGCTATT 
TTATACCCTG AGGAGGAGCC GTCGTGGACT CTGGCCAAAG ATGTGGTGTT CCAGCCGTAC 
TGGATGATGT ACGGGGAGGT GTACGCCTAC GAAATCGATG TGTGTGCGAA CTCGAGTGAG 
GAACATGTTC GGGATCTGTG TACGACTGGT GTGTGGCTGA CGCCGCTGCT GCAGGCCGTT 
TATCTGTTCG TCCAGTACAT CCTGATGGTC AACCTGCTGA TCGCCTTCTT CAATAACGTG 
TACCTGCAGG TGAAGTCGAT CTCTAACCTG GTGTGGAAGT ATCAGCGCTA TCACTTCATC 
CTAGCGTATC ATGATAAGCC CATCCTGCCG CCGCCGCTGA TCCTGCTGAG CCACGCCGCC 
TCGCTCCTCA GCTCCATCTG CAGGAAGAGG AAGAAGGACA GCAGCACCTA CGGGCCCAAG 
CTGTTCCTGA CCGAGGAGGA CCAGAAGAAG CTGCATGACT TCGAGGAGCA GTGTGTGGAG 
ACGTATTTCC ATGAGAAAGA CGATCAGTTC CACTCCGGCA GCGACGAGAG GATCAGACTA 
ACATCCGACA GGGTGGAGAC GATGTGTGTG CAGCTGAAGG AGGTTGGAAA TAAGGTGAAC 
TTCATCAAGC GCTCTCTGCA CACGCTGGAC TCTCAGATCG GGCACCTGCA GGATCTGTCT 
GCGCTGACGG TGGACACCCT GAAGGCTCTG ACGGCCCAGC GCGCATCTGA AGCCAGCAAA 
GTGCACAACC AGATCACACG TGAGCTCAGC GTGTCCCGAA ACCTGGCGCC CGAGGCCGTG 
CCGCCGTCCA AACCCGGCGC TCTGGTGCGC TGCAGCGTGG CCTTCATGCC GCCCAATAGC 
ATGGCGGACT CTCTGTTTGG GGGCGGGGTT GAGGCTGTGC AGGGGGCGGG GCTTCCTCAT 
GCTTCACTCA CCGCAGACGG TGTGCAGACA CCGCAGATGC GCCGCCGCGG ACACCAGGAA 
CATCTCTCCA GCGCTCCGGC TGCCGCCGCC AGCTTCTTCC TCAGCACGCC GCTGCCGCAG 
CAGCAGGACC TGCAGGCGCC GCCGGAGGAG TTCGGGGCGT TCGTGGGTCA CAAGGATGAG 
CCTGAGGGTC AAAGGTCAAT CCCTAAGGAG AGCGCTGTCA GCAGTCCATC CAGAAAGACC 
CGCGATGTGG GTAACATGCG GACGGTGAAC TCTTACGCCG GCTTCACAGA GTTGGACAGG 
AACCCTTCGC TCCTGCATCC AGAGTCCACA CTCAGTAAGC AGGAGAGGAC GCGCGTGTCG 
CTGGAGGACG TCTCTCGATA TGAGGATCTG CTGCTGGGGA AGTCCGCTCA GTCCAGCAAG 
CTGAGTCCAG GAGACGACGC CTCTAACACA GCGATCGGTT CTCCATTCAA GCCCATGGAG 
AGTTATCAGT ACTCAGCGGT GGAGCGTAAT AACCTGATGC GGTTGTCTCA GAGCATCCCC 
TTCACCCCGG TGCCCCCTAG AGGTGAGCCG GTGAGTGTGT ATCGGCTAGA GGAGAGCTCC 
CCCGGCAGTA TCAATAACAG CATGTCGTCG TGGGCTCAGC GCGGGCTCTG TGCGAAGATC 
GAGTTCCTCA GTAAGGAGGA GATGGGCGGG GGGCTGCGGC GCGCACTCAA GGTGCTCTGC 
ACCTGGTCCG AGTACGACAT CCTGAAGCCC GGACACCTGT ACATCGTCAA GTCCTTCCTG 
CCCGAGGTGG TCAACACCTG GCAGACCATC TACAGGGAGG AGACGGTGCT GCACCTCTGC 
CTCAGGGAAA TTCAGCAGCA GAGAGCAGCT CAGAAACTCA CGTTTGCCTT CAATCAGATC 
CGACCCAAAA CCATCCCGTA CTCGCCCAGG TTCCTGGAGG TCTTCCTGCT GTACTGTCAT 
TCTGCCGGTC AGTGGTTCGC CATCGAGGAG TGCATCACTG GAGATTTCCG CAAGTTTAAC 
AACAACAACG GAGATGAAAT AGTGCCGACC AACCTTCTGG AGGAAACCAT GCTGGCCTTC 
AGCCACTGGA CGTATGAATA CACACGAGGA GAGCTGCTGG TGCTGGACCT GCAGGGTGTG 
GGTGAGATTC TGACCGATCC GTCGGTCATC AAGAGCGGAG AGAAAGGGTC TTACGATATG 
ATCTTTGGAC CTGCAAACCT CGGAGACGAC GCCATTCGAA ACTTCCGTGC CAAACATCAC 
TGCAACTCCT GCTGCCGAAA ACTCAAACTT CCAGATCTGA AACGGAACGA CTACACTCCA 
GATAAGCTGA CCCTTCAGCA TGACCCCTCC GAGGGCCCGT CCCACTCTCC GCCTGCAGGG 
GCCGCCACTA AAGAACTGCG TCCCTCCATG CGCTTAATGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001025232.1
CDS	145..5469
Translation	MSQKSWIEHTFTRRECVYILPVSKDPHRCLPGCQICQQLVRCCCGRLVRQHVGFTATLAVKYSEVSEELSELEQWSVEKHTEESPTDAYGVINFQGGSHSYRAKYVRLSHDSRPEAVLRLMLKEWLMELPKIVISVHGGIQNFELHPRIKQVVGKGLIKAAVTTGAWILTGGVNTGVAKHVGDALKEHLSRSARKICTVGIAPWGVIENRNDLIGRDVVAPYQTLLNPLSKLNVLNNFHSHFLLVDDGTVGKYGAEVRLRRELEKHIQLQRIHARIGQGVPVVALIFEGGPNVILTVLEYLQESPPVPVVVCEGTGRAADILAYVHKQTEQDGALPDGVESDIIATIKKTFNFSHGDALHLFQTLMECMKNKELITVFPVGSEDHQDIDVAILKALLKGTNATAFDQLVLTLAWDRVDIAKDHVFVYGQQLLVGSLEQAMLDALVMDRVEFVKLLIENGVSMHRFLTITRLEELYNTKQTPTNPTLYHLVRDVKQGNLPPNYKITLIDVGLVIEYLMGGTYRCNYTRKRFRIIYNNLHGNSRRSGRHTSSSHSHESFSIQADKKEKTRHNHFIKTAQPYKPRLDSVSELQKKKSKEEVVDVDDPETRRFPYPFNELLVWAVLMKRQKMSLFFWQHGEESMAKALVACRLLRSLGDEAKKSDVVDDTSEELKEYSSEFGTLAVDLLEQSFRQDETMAMKLLTYELKNWSNSTCLKLAVSSRLRPFVAHTCTQMLLSDMWMGRLNMRKNSWYKVILSILVPPAILLLEYKTKAEMSHIPQSQDAHQSMEDSEHNLQHPDDIQMDVFKEVRMSDNAEMKSELEVHVRSRRLPITRKFYAFYHAPIVKFWFNTLFYIGFLMLYSFVVLVKMQDLPSPQEWVVILYIFTLAVEKIREMFMSEGGKISQKIKVWFSDYFNISDFLAILMFFVGFGLRLVSEVFIAGRIVYCLNIIFWYVRLLDILAVNQQAGPYVMMIGKMVANMFYIVVIMAVVLLSFGVPRKAILYPEEEPSWTLAKDVVFQPYWMMYGEVYAYEIDVCANSSEEHVRDLCTTGVWLTPLLQAVYLFVQYILMVNLLIAFFNNVYLQVKSISNLVWKYQRYHFILAYHDKPILPPPLILLSHAASLLSSICRKRKKDSSTYGPKLFLTEEDQKKLHDFEEQCVETYFHEKDDQFHSGSDERIRLTSDRVETMCVQLKEVGNKVNFIKRSLHTLDSQIGHLQDLSALTVDTLKALTAQRASEASKVHNQITRELSVSRNLAPEAVPPSKPGALVRCSVAFMPPNSMADSLFGGGVEAVQGAGLPHASLTADGVQTPQMRRRGHQEHLSSAPAAAASFFLSTPLPQQQDLQAPPEEFGAFVGHKDEPEGQRSIPKESAVSSPSRKTRDVGNMRTVNSYAGFTELDRNPSLLHPESTLSKQERTRVSLEDVSRYEDLLLGKSAQSSKLSPGDDASNTAIGSPFKPMESYQYSAVERNNLMRLSQSIPFTPVPPRGEPVSVYRLEESSPGSINNSMSSWAQRGLCAKIEFLSKEEMGGGLRRALKVLCTWSEYDILKPGHLYIVKSFLPEVVNTWQTIYREETVLHLCLREIQQQRAAQKLTFAFNQIRPKTIPYSPRFLEVFLLYCHSAGQWFAIEECITGDFRKFNNNNGDEIVPTNLLEETMLAFSHWTYEYTRGELLVLDLQGVGEILTDPSVIKSGEKGSYDMIFGPANLGDDAIRNFRAKHHCNSCCRKLKLPDLKRNDYTPDKLTLQHDPSEGPSHSPPAGAATKELRPSMRLML

Target ORF information:

RefSeq Version	NM_001030061.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily M, member 7 (trpm7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001030061.1

ORF Insert Sequence:

ATGTCCCAGA AGTCCTGGAT CGAACACACT TTCACCAGGA GGGAATGTGT CTATATTCTG 
CCCGTGTCCA AGGATCCGCA CAGATGCCTC CCAGGATGCC AGATCTGCCA GCAGCTCGTG 
CGGTGCTGCT GTGGGCGTCT GGTGCGTCAG CATGTGGGCT TCACGGCCAC ACTGGCGGTC 
AAGTACTCGG AGGTGTCGGA GGAGCTGTCG GAGCTGGAGC AGTGGAGCGT GGAGAAACAC 
ACAGAGGAGA GTCCCACTGA TGCGTACGGG GTCATCAACT TCCAGGGCGG ATCACACTCG 
TACAGGGCCA AGTATGTGCG TCTGTCACAT GACTCGCGTC CGGAGGCGGT GCTGCGGCTG 
ATGCTGAAGG AGTGGCTGAT GGAGCTGCCG AAGATCGTCA TCAGCGTTCA CGGAGGCATC 
CAGAACTTCG AGCTGCACCC GCGCATCAAA CAGGTGGTGG GGAAGGGCCT GATCAAAGCT 
GCTGTCACCA CTGGAGCCTG GATCCTGACC GGAGGGGTCA ACACAGGTGT GGCCAAACAT 
GTGGGTGATG CGCTGAAGGA ACATCTGTCC AGATCGGCTC GGAAAATCTG TACGGTGGGA 
ATCGCACCCT GGGGAGTGAT CGAAAACAGG AACGACCTCA TCGGACGAGA CGTTGTAGCA 
CCGTATCAAA CGCTGCTGAA TCCTCTCAGT AAACTCAACG TCCTCAATAA CTTCCACTCG 
CACTTCCTGC TGGTGGACGA CGGCACTGTG GGCAAATATG GAGCTGAAGT CAGACTGAGG 
AGAGAGCTGG AGAAACACAT CCAACTGCAG AGGATACACG CACGCATTGG TCAGGGTGTT 
CCAGTGGTGG CGTTGATCTT TGAAGGGGGG CCGAATGTGA TCCTGACGGT GCTGGAGTAT 
CTGCAGGAGA GCCCGCCGGT GCCCGTGGTG GTGTGTGAGG GCACGGGACG CGCTGCGGAC 
ATACTGGCAT ACGTGCACAA GCAGACTGAG CAAGATGGTG CGCTGCCTGA CGGTGTGGAG 
TCCGACATCA TTGCAACAAT CAAGAAAACC TTCAACTTCA GTCACGGCGA CGCGCTGCAC 
CTGTTCCAGA CACTGATGGA GTGTATGAAG AACAAAGAGC TGATCACTGT GTTTCCTGTG 
GGCTCTGAGG ATCATCAGGA CATTGATGTA GCGATTCTGA AGGCTCTCCT CAAAGGCACA 
AATGCGACAG CGTTCGATCA GCTGGTGTTG ACGCTGGCGT GGGATCGCGT GGACATCGCT 
AAAGATCACG TGTTTGTTTA CGGACAGCAG CTGCTGGTGG GTTCTCTAGA GCAGGCTATG 
CTGGATGCAT TAGTGATGGA CCGTGTGGAG TTCGTCAAAC TGCTGATAGA GAACGGCGTC 
AGCATGCACA GATTCCTGAC CATCACACGC CTGGAGGAGC TCTACAACAC GAAACAAACA 
CCAACAAACC CGACGCTGTA TCACCTGGTC AGAGACGTCA AACAGGGGAA TCTCCCGCCC 
AACTATAAGA TCACACTGAT TGACGTTGGT CTGGTGATCG AGTATCTGAT GGGAGGAACG 
TACAGATGCA ACTACACACG CAAACGCTTC CGCATCATCT ACAACAACCT GCACGGGAAC 
AGCAGACGGT CGGGGCGACA CACCAGCAGC TCTCACTCTC ATGAGTCCTT CAGCATTCAG 
GCAGATAAGA AGGAGAAAAC CAGACACAAC CACTTCATCA AGACCGCACA GCCGTACAAG 
CCCAGGCTGG ACTCGGTGTC AGAGCTGCAG AAGAAGAAGA GTAAGGAGGA GGTGGTGGAC 
GTGGACGACC CGGAGACGCG GCGCTTCCCG TACCCGTTCA ACGAGCTGCT GGTGTGGGCG 
GTGCTGATGA AGCGGCAGAA GATGAGTCTG TTCTTCTGGC AGCACGGGGA GGAGTCCATG 
GCCAAAGCGC TGGTGGCCTG CAGACTGCTG CGCTCGCTCG GGGACGAGGC CAAGAAGAGC 
GACGTGGTGG ACGACACCTC CGAGGAGCTC AAGGAGTACT CCAGTGAGTT CGGCACGCTG 
GCGGTGGACC TGCTGGAGCA GTCGTTCCGT CAGGACGAGA CGATGGCGAT GAAGCTGTTG 
ACGTACGAGC TGAAGAACTG GAGCAACTCC ACCTGTCTGA AGCTGGCGGT GTCGTCACGG 
TTACGGCCCT TCGTGGCACA CACCTGCACA CAGATGCTGC TGTCCGACAT GTGGATGGGC 
AGACTCAACA TGCGCAAGAA CTCCTGGTAC AAGGTGATCT TGAGTATCCT GGTTCCTCCG 
GCCATCCTCC TGCTGGAGTA CAAGACCAAA GCGGAAATGT CCCACATTCC CCAGAGTCAG 
GACGCACACC AGAGCATGGA GGACAGCGAA CACAACCTGC AGCACCCAGA CGACATACAG 
ATGGATGTGT TTAAGGAAGT GCGTATGAGT GATAATGCAG AAATGAAGAG TGAGTTGGAG 
GTGCACGTGC GCTCCCGCAG GTTACCCATC ACACGCAAGT TCTACGCCTT CTACCACGCG 
CCCATCGTCA AGTTCTGGTT CAACACGCTG TTCTACATCG GTTTCCTGAT GCTCTACTCA 
TTCGTGGTTC TGGTGAAGAT GCAGGATCTT CCGTCTCCTC AGGAGTGGGT CGTCATTCTC 
TACATCTTCA CACTCGCGGT GGAGAAGATA CGAGAGATGT TCATGTCTGA GGGAGGAAAG 
ATCAGTCAGA AGATTAAGGT CTGGTTCAGC GATTACTTCA ACATCTCTGA TTTCCTGGCC 
ATCCTGATGT TCTTCGTGGG CTTCGGTCTG CGTCTGGTGT CGGAGGTGTT CATCGCGGGC 
CGCATCGTGT ACTGCCTGAA CATCATCTTC TGGTACGTGC GGCTGCTGGA CATCCTGGCG 
GTCAACCAGC AGGCCGGCCC GTACGTCATG ATGATCGGCA AGATGGTGGC CAACATGTTC 
TACATCGTGG TCATCATGGC GGTGGTGCTG CTGAGCTTCG GTGTTCCCCG TAAAGCTATT 
TTATACCCTG AGGAGGAGCC GTCGTGGACT CTGGCCAAAG ATGTGGTGTT CCAGCCGTAC 
TGGATGATGT ACGGGGAGGT GTACGCCTAC GAAATCGATG TGTGTGCGAA CTCGAGTGAG 
GAACATGTTC GGGATCTGTG TACGACTGGT GTGTGGCTGA CGCCGCTGCT GCAGGCCGTT 
TATCTGTTCG TCCAGTACAT CCTGATGGTC AACCTGCTGA TCGCCTTCTT CAATAACGTG 
TACCTGCAGG TGAAGTCGAT CTCTAACCTG GTGTGGAAGT ATCAGCGCTA TCACTTCATC 
CTAGCGTATC ATGATAAGCC CATCCTGCCG CCGCCGCTGA TCCTGCTGAG CCACGCCGCC 
TCGCTCCTCA GCTCCATCTG CAGGAAGAGG AAGAAGGACA GCAGCACCTA CGGGCCCAAG 
CTGTTCCTGA CCGAGGAGGA CCAGAAGAAG CTGCATGACT TCGAGGAGCA GTGTGTGGAG 
ACGTATTTCC ATGAGAAAGA CGATCAGTTC CACTCCGGCA GCGACGAGAG GATCAGACTA 
ACATCCGACA GGGTGGAGAC GATGTGTGTG CAGCTGAAGG AGGTTGGAAA TAAGGTGAAC 
TTCATCAAGC GCTCTCTGCA CACGCTGGAC TCTCAGATCG GGCACCTGCA GGATCTGTCT 
GCGCTGACGG TGGACACCCT GAAGGCTCTG ACGGCCCAGC GCGCATCTGA AGCCAGCAAA 
GTGCACAACC AGATCACACG TGAGCTCAGC GTGTCCCGAA ACCTGGCGCC CGAGGCCGTG 
CCGCCGTCCA AACCCGGCGC TCTGGTGCGC TGCAGCGTGG CCTTCATGCC GCCCAATAGC 
ATGGCGGACT CTCTGTTTGG GGGCGGGGTT GAGGCTGTGC AGGGGGCGGG GCTTCCTCAT 
GCTTCACTCA CCGCAGACGG TGTGCAGACA CCGCAGATGC GCCGCCGCGG ACACCAGGAA 
CATCTCTCCA GCGCTCCGGC TGCCGCCGCC AGCTTCTTCC TCAGCACGCC GCTGCCGCAG 
CAGCAGGACC TGCAGGCGCC GCCGGAGGAG TTCGGGGCGT TCGTGGGTCA CAAGGATGAG 
CCTGAGGGTC AAAGGTCAAT CCCTAAGGAG AGCGCTGTCA GCAGTCCATC CAGAAAGACC 
CGCGATGTGG GTAACATGCG GACGGTGAAC TCTTACGCCG GCTTCACAGA GTTGGACAGG 
AACCCTTCGC TCCTGCATCC AGAGTCCACA CTCAGTAAGC AGGAGAGGAC GCGCGTGTCG 
CTGGAGGACG TCTCTCGATA TGAGGATCTG CTGCTGGGGA AGTCCGCTCA GTCCAGCAAG 
CTGAGTCCAG GAGACGACGC CTCTAACACA GCGATCGGTT CTCCATTCAA GCCCATGGAG 
AGTTATCAGT ACTCAGCGGT GGAGCGTAAT AACCTGATGC GGTTGTCTCA GAGCATCCCC 
TTCACCCCGG TGCCCCCTAG AGGTGAGCCG GTGAGTGTGT ATCGGCTAGA GGAGAGCTCC 
CCCGGCAGTA TCAATAACAG CATGTCGTCG TGGGCTCAGC GCGGGCTCTG TGCGAAGATC 
GAGTTCCTCA GTAAGGAGGA GATGGGCGGG GGGCTGCGGC GCGCACTCAA GGTGCTCTGC 
ACCTGGTCCG AGTACGACAT CCTGAAGCCC GGACACCTGT ACATCGTCAA GTCCTTCCTG 
CCCGAGGTGG TCAACACCTG GCAGACCATC TACAGGGAGG AGACGGTGCT GCACCTCTGC 
CTCAGGGAAA TTCAGCAGCA GAGAGCAGCT CAGAAACTCA CGTTTGCCTT CAATCAGATC 
CGACCCAAAA CCATCCCGTA CTCGCCCAGG TTCCTGGAGG TCTTCCTGCT GTACTGTCAT 
TCTGCCGGTC AGTGGTTCGC CATCGAGGAG TGCATCACTG GAGATTTCCG CAAGTTTAAC 
AACAACAACG GAGATGAAAT AGTGCCGACC AACCTTCTGG AGGAAACCAT GCTGGCCTTC 
AGCCACTGGA CGTATGAATA CACACGAGGA GAGCTGCTGG TGCTGGACCT GCAGGGTGTG 
GGTGAGATTC TGACCGATCC GTCGGTCATC AAGAGCGGAG AGAAAGGGTC TTACGATATG 
ATCTTTGGAC CTGCAAACCT CGGAGACGAC GCCATTCGAA ACTTCCGTGC CAAACATCAC 
TGCAACTCCT GCTGCCGAAA ACTCAAACTT CCAGATCTGA AACGGAACGA CTACACTCCA 
GATAAGCTGA CCCTTCAGCA TGACCCCTCC GAGGGCCCGT CCCACTCTCC GCCTGCAGGG 
GCCGCCACTA AAGAACTGCG TCCCTCCATG CGCTTAATGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The tbx2a/b transcription factors direct pronephros segmentation and corpuscle of Stannius formation in zebrafish.
Developmental biology421(1)52-66(2017 Jan)
Drummond BE,Li Y,Marra AN,Cheng CN,Wingert RA

Prostaglandin signaling regulates nephron segment patterning of renal progenitors during zebrafish kidney development.
eLife5(2016 12)
Poureetezadi SJ,Cheng CN,Chambers JM,Drummond BE,Wingert RA

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trpm7 cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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