pwp2h cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	pwp2h
Entrez Gene ID	266990
Full Name	PWP2 periodic tryptophan protein homolog (yeast)
Synonyms	cb471,zgc:56063
General protein information	Preferred Names PWP2 periodic tryptophan protein homolog (yeast) Names periodic tryptophan protein 2 homolog titania tti
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_213047.1	NP_998212.1	periodic tryptophan protein 2 homolog

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PWP2	XP_531577.2
	PWP2	XP_001118393.2
Mus musculus (house mouse)	Pwp2	NP_083822.1
Gallus gallus (chicken)	PWP2	XP_416757.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010693	XP_311407.4
Saccharomyces cerevisiae (baker's yeast)	PWP2	NP_009984.1
Eremothecium gossypii	AGOS_AAR057W	NP_982598.1
Arabidopsis thaliana (thale cress)	PWP2	NP_172998.1
Oryza sativa (rice)	Os05g0519500	NP_001056064.1
Rattus norvegicus (Norway rat)	Pwp2	NP_001162124.1
Drosophila melanogaster (fruit fly)	CG12325	NP_610618.1
Caenorhabditis elegans (roundworm)	F55F8.3	NP_491653.2
Neurospora crassa	NCU03794	XP_961183.1
Xenopus tropicalis (tropical clawed frog)	LOC100158563	NP_001121467.1
Danio rerio (zebrafish)	pwp2h	NP_998212.1
Kluyveromyces lactis	KLLA0C08976g	XP_452599.1
Canis lupus familiaris (dog)	PWP2	XP_544915.4
Bos taurus (cattle)	PWP2	XP_002685190.1
Schizosaccharomyces pombe (fission yeast)	SPBC713.04c	NP_595343.1
Magnaporthe oryzae (rice blast fungus)	MGG_07465	XP_003711369.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Autophagy induction is a Tor- and Tp53-independent cell survival response in a zebrafish model of disrupted ribosome biogenesis.
Boglev Y, Badrock AP, Trotter AJ, Du Q, Richardson EJ, Parslow AC, Markmiller SJ, Hall NE, de Jong-Curtain TA, Ng AY, Verkade H, Ober EA, Field HA, Shin D, Shin CH, Hannan KM, Hannan RD, Pearson RB, Kim SH, Ess KC, Lieschke GJ, Stainier DY, Heath JK
PLoS genetics9(2)e1003279(2013)

Live imaging of endogenous periodic tryptophan protein 2 gene homologue during zebrafish development.
Jayasena CS, Trinh le A, Bronner M
Developmental dynamics : an official publication of the American Association of Anatomists240(11)2578-83(2011 Nov)

Expression screening and annotation of a zebrafish myoblast cDNA library.
Baxendale S, Chen CK, Tang H, Davison C, Hateren LV, Croning MD, Humphray SJ, Hubbard SJ, Ingham PW
Gene expression patterns : GEP9(2)73-82(2009 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

pwp2h-deficient model, titania (ttis450), the growth of the endodermal organs, eyes, brain and craniofacial structures is severely arrested and autophagy is markedly up-regulated
Title: Autophagy induction is a Tor- and Tp53-independent cell survival response in a zebrafish model of disrupted ribosome biogenesis.

At the subcellular level, Pwp2h protein appears to be localized to the region of the nucleolus consistent with its presumed function in ribosomal RNA synthesis.
Title: Live imaging of endogenous periodic tryptophan protein 2 gene homologue during zebrafish development.

The following pwp2h gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pwp2h cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa37002	NM_213047.1 Latest version!	Danio rerio PWP2 small subunit processome component (pwp2h), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pwp2h ( NM_213047.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_998212.1 Danio rerio PWP2 small subunit processome component (pwp2h), mRNA.

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CloneID	ODa37002
Clone ID Related Accession (Same CDS sequence)	NM_213047.1
Accession Version	NM_213047.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2814bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-07
Organism	Danio rerio(zebrafish)
Product	periodic tryptophan protein 2 homolog
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC047817.1. ##Evidence-Data-START## Transcript exon combination :: BC047817.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGAAGTTTG CGTACAAGTT TTCAAACTTA TTAGGGGCAT TTTATCGCCA AGGGAACATA 
TCTTTCTCTA AAAATGGGGA TTCTGTCATC AGTCCTGTAG GCAACCGCAT CTCCGTCTTT 
GATCTGAAAA ATAACAAGTC AGAAACCTTA CCGGTGTCCA CCTCAAAGAA CATTGCTTGT 
TTTGACATCT CTCCAGACGG AAATCTTGCT ATACTAATAG ATGAAGATGG AGCGGCAATA 
CTGGTTAGCT TGGTCACTCG GGCAGTACTT CACCATCATC ACTTCCATAA TCCTGTTCAT 
AATGTTTCCT TTTCCCCTGA TGGAAGGAAG TTTGTGGTAA CAAAGGAGAA TGTGGCTCTA 
ATGTATCATG CACCGGGAAG AAACAAAGAA TTTAATGCAT TTGCATTAAA CAAGACTTTT 
TATGGACCAT TTGATGAAAT GACTTGTATT GACTGGACAG ACGATTCGAA GTGCTTTGCT 
GTTGGAAGTA AGGACATGAC AACCTGGATC TTTGGGGCAG AAAGATGGGC AAATCTCATC 
TACTACTCAC TTGGTGGTCA TAAGGACATC ATAGTGGGCT GTTTCTTTGA AAAAGACAGC 
TTGGATATGT ACACAGTAAG TCAGGAAGGC ACTTTATGTA TATGGGAAAG TGACACTGAT 
CTAGAAGGCT TACGAAAGGG CCCAAAATAC GCTGAGAGAA AGGAAAAGGA GCAGAAAAAA 
CTGAGAGTTT TAGAAATGGA GGAAGATGAA GCTAATGACA TTGAGATTAT GGGGGAGGAT 
GAAGAGACCA GAGGAGATGT CATCAAAGGA AGTGCCGAAA CACCTGAAGA AGTGGAAGGC 
ATAAAAAATG TTCGTTATTT ACAGAAAAGC AAATACTATT TTAACAAAGA AGGGGATTTC 
AACAAACTGA CAGCTGCCGC CTTTCACAAG AGAACACACA TACTTGTGAC AGGGTTTGCA 
TCTGGAGCTT TTCACCTGCA TGAATTGCCA GGTTTTGACC TCATTCACTC ACTCAGCATT 
TCAGATCAGA GGATTTCGGC CATTTCTCTG AATCCCACTG GTGACTGGAT TAGCTTTGGC 
TGCTCAGGTC TGGGCCAGCT GTTGGTTTGG GAATGGCAGA GCGAGTCCTA TGTGTTTAAA 
CAGCAAGGAC ACTTTAACAA CATGAACTCT TTGGCTTATT CACCTGATGG TCAGTATCTT 
GCTACTGGAG GGGATGATGG CAAGGTGAAG GTGTGGAACA CCAACAGTGG TCTCTGTTTT 
GTGACATTCA CCGAGCACTC CAGCGGTGTC ACCGAAGTTG CCTTTACCTC CAGTGGTTTC 
GTGGTGGTCA GTGCCTCACT AGATGGCACT GTTAGAGCTT TTGACCTGCA CAGGTACCGG 
AATTTCCGTA CCATGACGTC ACCACGACCC GCCCAGTTCT CCTCTCTGGC AGTGGACGGT 
AGTGGGGAAT TGGTGTGTGC TGGTTCTCAG GACTCTTTTG AGATCTTTCT GTGGTCTATG 
CAGACTGGAC GACTGTTAGA GGTGCTTAGT GGTCATGAGG GTCCTGTCAG TAACTTGTGC 
TTCAGTCCAG TCCAGTCTGT TCTTGCTAGT GTGTCATGGG ACAAAACTGT CCGGCTATGG 
GACATGTTGG ACAGCTGGCA GACCAAAGAG ACGTTGCAAC TCACCTCTGA TGGGCTTGCT 
GTAACATATC ATCCTAATGG TATGGAGTTA GCAGTTGCTT CTCTGGATGG TGAAATCACA 
TTCTGGAACC CTCAGACTGG AAGACAGACT GGCTCTATCA CTGGCCGCCA TGACCTGCAA 
ATGGGACGCA AAGAGTCTGA AAAGATCACT GCTAAACAAT CTGCCAAGGG AAAGTCTTTT 
ACCACCTTGT GCTACTCAGC GGATGGTGAA TCGATCCTTA CAGGTGGGAA GTCAAAGTTT 
GTGTGCATTT ATAACATCAA GGAACATCTC CTGATGAAGA AGTTTGAGAT CTCCTGTAAT 
CTCTCTCTGG ATGCAATGGA GGAATTCCTT GACAGGCGTA AGATGACCGA GTTTGGTAGT 
CTGTCTTTAG TGGATGAAGG CACTGGGGAT GGAAACGGAG TGGAGCTCAG TCTTCCTGGA 
GTCAAGAAAG GTGATATGAG CACCCGATGC TTTAAACCAG AGATCAGTGT TAGCTCTCTT 
CGCTTCTCAC CTACTGGGCG CAGTTGGGCT GCAGCATCCA CTGAAGGTTT GCTGATCTAC 
TCTCTTGATG CTGCACTAGT GTTTGACCCT TATGATCTGG ATATTGATGT CACACCAACA 
AGTATACGTC AGCAGATTAC CAAAAAAGAG TGGGCTTCCG CCATCTTGCT GTTTTTTCGC 
CTAAATGAAA TTCTGCTCAT TAGAGAAGTT CTGGAGGCAA TCCCTTTTGA TCAAATTACA 
GTTGTATGCA GCTCATTGCC AGATGTGTAT GTGGATAAGC TTCTAAACTT TGTGGCCTCA 
ACACTGGAAA AGTCTAACCA CCTGCAGTTC TACCTTACCT GGGCTCAGTG TTTGCTCACT 
TTACATGGAC AGAAACTCAA GAATCGACCT GGATTTGTAC TGCCTACTGT ACAGCAGCTG 
CTGAAGAGCA TCCAAAGACA CAGTGATGAT CTGTCTAAAC TTTGCGATTG GAATATTTAC 
AACATTCGCT ACGCTGCTGC TCTGGCTAAG CAGAGAGGAC TGAAAAGAAC AGCATCTGAG 
ATCTACGGTG CTGAAAAAGA GGACTCATCA GAGGCAATGG ATTCAGAGGG TGATGAGGAG 
AATGTGACAA ATGAGCCTAT AATGCTGGAA AATGCCATTC AACTTTCAGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_998212.1
CDS	87..2900
Translation	MKFAYKFSNLLGAFYRQGNISFSKNGDSVISPVGNRISVFDLKNNKSETLPVSTSKNIACFDISPDGNLAILIDEDGAAILVSLVTRAVLHHHHFHNPVHNVSFSPDGRKFVVTKENVALMYHAPGRNKEFNAFALNKTFYGPFDEMTCIDWTDDSKCFAVGSKDMTTWIFGAERWANLIYYSLGGHKDIIVGCFFEKDSLDMYTVSQEGTLCIWESDTDLEGLRKGPKYAERKEKEQKKLRVLEMEEDEANDIEIMGEDEETRGDVIKGSAETPEEVEGIKNVRYLQKSKYYFNKEGDFNKLTAAAFHKRTHILVTGFASGAFHLHELPGFDLIHSLSISDQRISAISLNPTGDWISFGCSGLGQLLVWEWQSESYVFKQQGHFNNMNSLAYSPDGQYLATGGDDGKVKVWNTNSGLCFVTFTEHSSGVTEVAFTSSGFVVVSASLDGTVRAFDLHRYRNFRTMTSPRPAQFSSLAVDGSGELVCAGSQDSFEIFLWSMQTGRLLEVLSGHEGPVSNLCFSPVQSVLASVSWDKTVRLWDMLDSWQTKETLQLTSDGLAVTYHPNGMELAVASLDGEITFWNPQTGRQTGSITGRHDLQMGRKESEKITAKQSAKGKSFTTLCYSADGESILTGGKSKFVCIYNIKEHLLMKKFEISCNLSLDAMEEFLDRRKMTEFGSLSLVDEGTGDGNGVELSLPGVKKGDMSTRCFKPEISVSSLRFSPTGRSWAAASTEGLLIYSLDAALVFDPYDLDIDVTPTSIRQQITKKEWASAILLFFRLNEILLIREVLEAIPFDQITVVCSSLPDVYVDKLLNFVASTLEKSNHLQFYLTWAQCLLTLHGQKLKNRPGFVLPTVQQLLKSIQRHSDDLSKLCDWNIYNIRYAAALAKQRGLKRTASEIYGAEKEDSSEAMDSEGDEENVTNEPIMLENAIQLSD

Target ORF information:

RefSeq Version	NM_213047.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio PWP2 small subunit processome component (pwp2h), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_213047.1

ORF Insert Sequence:

ATGAAGTTTG CGTACAAGTT TTCAAACTTA TTAGGGGCAT TTTATCGCCA AGGGAACATA 
TCTTTCTCTA AAAATGGGGA TTCTGTCATC AGTCCTGTAG GCAACCGCAT CTCCGTCTTT 
GATCTGAAAA ATAACAAGTC AGAAACCTTA CCGGTGTCCA CCTCAAAGAA CATTGCTTGT 
TTTGACATCT CTCCAGACGG AAATCTTGCT ATACTAATAG ATGAAGATGG AGCGGCAATA 
CTGGTTAGCT TGGTCACTCG GGCAGTACTT CACCATCATC ACTTCCATAA TCCTGTTCAT 
AATGTTTCCT TTTCCCCTGA TGGAAGGAAG TTTGTGGTAA CAAAGGAGAA TGTGGCTCTA 
ATGTATCATG CACCGGGAAG AAACAAAGAA TTTAATGCAT TTGCATTAAA CAAGACTTTT 
TATGGACCAT TTGATGAAAT GACTTGTATT GACTGGACAG ACGATTCGAA GTGCTTTGCT 
GTTGGAAGTA AGGACATGAC AACCTGGATC TTTGGGGCAG AAAGATGGGC AAATCTCATC 
TACTACTCAC TTGGTGGTCA TAAGGACATC ATAGTGGGCT GTTTCTTTGA AAAAGACAGC 
TTGGATATGT ACACAGTAAG TCAGGAAGGC ACTTTATGTA TATGGGAAAG TGACACTGAT 
CTAGAAGGCT TACGAAAGGG CCCAAAATAC GCTGAGAGAA AGGAAAAGGA GCAGAAAAAA 
CTGAGAGTTT TAGAAATGGA GGAAGATGAA GCTAATGACA TTGAGATTAT GGGGGAGGAT 
GAAGAGACCA GAGGAGATGT CATCAAAGGA AGTGCCGAAA CACCTGAAGA AGTGGAAGGC 
ATAAAAAATG TTCGTTATTT ACAGAAAAGC AAATACTATT TTAACAAAGA AGGGGATTTC 
AACAAACTGA CAGCTGCCGC CTTTCACAAG AGAACACACA TACTTGTGAC AGGGTTTGCA 
TCTGGAGCTT TTCACCTGCA TGAATTGCCA GGTTTTGACC TCATTCACTC ACTCAGCATT 
TCAGATCAGA GGATTTCGGC CATTTCTCTG AATCCCACTG GTGACTGGAT TAGCTTTGGC 
TGCTCAGGTC TGGGCCAGCT GTTGGTTTGG GAATGGCAGA GCGAGTCCTA TGTGTTTAAA 
CAGCAAGGAC ACTTTAACAA CATGAACTCT TTGGCTTATT CACCTGATGG TCAGTATCTT 
GCTACTGGAG GGGATGATGG CAAGGTGAAG GTGTGGAACA CCAACAGTGG TCTCTGTTTT 
GTGACATTCA CCGAGCACTC CAGCGGTGTC ACCGAAGTTG CCTTTACCTC CAGTGGTTTC 
GTGGTGGTCA GTGCCTCACT AGATGGCACT GTTAGAGCTT TTGACCTGCA CAGGTACCGG 
AATTTCCGTA CCATGACGTC ACCACGACCC GCCCAGTTCT CCTCTCTGGC AGTGGACGGT 
AGTGGGGAAT TGGTGTGTGC TGGTTCTCAG GACTCTTTTG AGATCTTTCT GTGGTCTATG 
CAGACTGGAC GACTGTTAGA GGTGCTTAGT GGTCATGAGG GTCCTGTCAG TAACTTGTGC 
TTCAGTCCAG TCCAGTCTGT TCTTGCTAGT GTGTCATGGG ACAAAACTGT CCGGCTATGG 
GACATGTTGG ACAGCTGGCA GACCAAAGAG ACGTTGCAAC TCACCTCTGA TGGGCTTGCT 
GTAACATATC ATCCTAATGG TATGGAGTTA GCAGTTGCTT CTCTGGATGG TGAAATCACA 
TTCTGGAACC CTCAGACTGG AAGACAGACT GGCTCTATCA CTGGCCGCCA TGACCTGCAA 
ATGGGACGCA AAGAGTCTGA AAAGATCACT GCTAAACAAT CTGCCAAGGG AAAGTCTTTT 
ACCACCTTGT GCTACTCAGC GGATGGTGAA TCGATCCTTA CAGGTGGGAA GTCAAAGTTT 
GTGTGCATTT ATAACATCAA GGAACATCTC CTGATGAAGA AGTTTGAGAT CTCCTGTAAT 
CTCTCTCTGG ATGCAATGGA GGAATTCCTT GACAGGCGTA AGATGACCGA GTTTGGTAGT 
CTGTCTTTAG TGGATGAAGG CACTGGGGAT GGAAACGGAG TGGAGCTCAG TCTTCCTGGA 
GTCAAGAAAG GTGATATGAG CACCCGATGC TTTAAACCAG AGATCAGTGT TAGCTCTCTT 
CGCTTCTCAC CTACTGGGCG CAGTTGGGCT GCAGCATCCA CTGAAGGTTT GCTGATCTAC 
TCTCTTGATG CTGCACTAGT GTTTGACCCT TATGATCTGG ATATTGATGT CACACCAACA 
AGTATACGTC AGCAGATTAC CAAAAAAGAG TGGGCTTCCG CCATCTTGCT GTTTTTTCGC 
CTAAATGAAA TTCTGCTCAT TAGAGAAGTT CTGGAGGCAA TCCCTTTTGA TCAAATTACA 
GTTGTATGCA GCTCATTGCC AGATGTGTAT GTGGATAAGC TTCTAAACTT TGTGGCCTCA 
ACACTGGAAA AGTCTAACCA CCTGCAGTTC TACCTTACCT GGGCTCAGTG TTTGCTCACT 
TTACATGGAC AGAAACTCAA GAATCGACCT GGATTTGTAC TGCCTACTGT ACAGCAGCTG 
CTGAAGAGCA TCCAAAGACA CAGTGATGAT CTGTCTAAAC TTTGCGATTG GAATATTTAC 
AACATTCGCT ACGCTGCTGC TCTGGCTAAG CAGAGAGGAC TGAAAAGAAC AGCATCTGAG 
ATCTACGGTG CTGAAAAAGA GGACTCATCA GAGGCAATGG ATTCAGAGGG TGATGAGGAG 
AATGTGACAA ATGAGCCTAT AATGCTGGAA AATGCCATTC AACTTTCAGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Autophagy induction is a Tor- and Tp53-independent cell survival response in a zebrafish model of disrupted ribosome biogenesis.
PLoS genetics9(2)e1003279(2013)
Boglev Y,Badrock AP,Trotter AJ,Du Q,Richardson EJ,Parslow AC,Markmiller SJ,Hall NE,de Jong-Curtain TA,Ng AY,Verkade H,Ober EA,Field HA,Shin D,Shin CH,Hannan KM,Hannan RD,Pearson RB,Kim SH,Ess KC,Lieschke GJ,Stainier DY,Heath JK

Live imaging of endogenous periodic tryptophan protein 2 gene homologue during zebrafish development.
Developmental dynamics : an official publication of the American Association of Anatomists240(11)2578-83(2011 Nov)
Jayasena CS,Trinh le A,Bronner M

Expression screening and annotation of a zebrafish myoblast cDNA library.
Gene expression patterns : GEP9(2)73-82(2009 Feb)
Baxendale S,Chen CK,Tang H,Davison C,Hateren LV,Croning MD,Humphray SJ,Hubbard SJ,Ingham PW

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pwp2h cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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