npm1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	npm1a
Entrez Gene ID	266985
Full Name	nucleophosmin 1a
Synonyms	npm1,sb:cb487
General protein information	Preferred Names nucleophosmin 1a Names nucleophosmin nucleophosmin 1 nucleophosmin 1a (nucleolar phosphoprotein B23, numatrin)
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_199428.2	NP_955460.2	nucleophosmin

Pathways from BioSystems

Homologies

	LOC706883	XP_002804677.1
Bos taurus (cattle)	NPM1	NP_001030518.1
Rattus norvegicus (Norway rat)	Npm1	NP_037124.1
Gallus gallus (chicken)	NPM1	NP_990598.1
Mus musculus (house mouse)	Npm1	NP_032748.1
Danio rerio (zebrafish)	npm1a	NP_955460.1
Homo sapiens (human)	NPM1	NP_002511.1
Pan troglodytes (chimpanzee)	NPM1	NP_001238905.1
Xenopus tropicalis (tropical clawed frog)	npm1	NP_988883.1
Canis lupus familiaris (dog)	NPM1	NP_001239100.1

Gene Ontology
Bibliography

Related articles in PubMed

Cross talk between TP53 and c-Myc in the pathophysiology of Diamond-Blackfan anemia: Evidence from RPL11-deficient in?vivo and in?vitro models.
Chakraborty A, Uechi T, Nakajima Y, Gazda HT, O'Donohue MF, Gleizes PE, Kenmochi N
Biochemical and biophysical research communications495(2)1839-1845(2018 Jan)

NPM1 directs PIDDosome-dependent caspase-2 activation in the nucleolus.
Ando K, Parsons MJ, Shah RB, Charendoff CI, Paris SL, Liu PH, Fassio SR, Rohrman BA, Thompson R, Oberst A, Sidi S, Bouchier-Hayes L
The Journal of cell biology216(6)1795-1810(2017 Jun)

Maternal methylmercury from a wild-caught walleye diet induces developmental abnormalities in zebrafish.
Liu Q, Klingler RH, Wimpee B, Dellinger M, King-Heiden T, Grzybowski J, Gerstenberger SL, Weber DN, Carvan MJ 3rd
Reproductive toxicology (Elmsford, N.Y.)65272-282(2016 10)

High efficiency In Vivo genome engineering with a simplified 15-RVD GoldyTALEN design.
Ma AC, Lee HB, Clark KJ, Ekker SC
PloS one8(5)e65259(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following npm1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the npm1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa39596	NM_199428.2 Latest version!	Danio rerio nucleophosmin 1a (npm1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

npm1a ( NM_199428.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_955460.2 Danio rerio nucleophosmin 1a (npm1a), mRNA.

Price & Availability↑

CloneID	ODa39596
Clone ID Related Accession (Same CDS sequence)	NM_199428.2
Accession Version	NM_199428.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-30
Organism	Danio rerio(zebrafish)
Product	nucleophosmin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX005027.13. On Aug 20, 2015 this sequence version replaced NM_199428.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC053240.1, CN016602.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476743, SAMEA4476768 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATCTCG AACAGATGGG TCCCCAAACC TTCCTGTACG GTTGTGAACT GAAAGCAGGC 
AAGGATATCA CGTTTAATCC AGAGGATGAT GATTATGATC ATCAATTATC GGTTAGAATG 
GCCTGTGTGG ATCCCTCGAC AAAAGATGAA TTGAATGTTG TGGAGATTGA AGGACAAGAT 
TCAGAGGGTC AGAAGGTCAA GGCAGTTCTA GCAACACTCA AGCCTTCAAC TCTCCCAAGT 
GTATGTTTGG GTGGGTTTGA GATCACGCCA CCGGTTGTTT TCCGTCTGCG CACTGGTTCT 
GGTCCAGTTC ACATTAGTGG ACAGCATCTT GTTATCATGG GAGGAGATCA GTCTTTTGAT 
GAAGAGGAGG AGGAGGAGGA AGAAGAGGAA ACCGTGATGA CATCCAAGAA GAGGCCAGCG 
CTGTCAACTC CAAAGCCTTC AAAAAAAATG AAGATGGATG AGGAAGATGA CGATGATGAT 
GATGAGGACG ATGATGATGA AGATGACGAT GAGGAAGATG AAAGTGAAGA GGAGTCACCT 
GTGAAAGAAA AGAAAGCACC AGCGAAACCA AAGACCCCTA CACAGAATGG AAAAGGACCC 
AAACCCAGTA CACCTGCTAA ACAAACTCCA CAAAAGGGCA AGAAGGAACA GACACCCAAA 
ACGCCACAAA CACCACGGAC TTTAGCCGAT ATCAAGTCCA AGATGATGGA GAGTGTAGCA 
AAGGGTGTGT CATTACCAAA AGTACAGCTG AAGTTTGAGA ACTATGTGAA TAACTGCTTC 
AAAGGCACAG ATCCAAAGGT TGTTGAGGAG CTCTGGAAGT GGAGACAGAC TGTCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_955460.2
CDS	134..973
Translation	MDLEQMGPQTFLYGCELKAGKDITFNPEDDDYDHQLSVRMACVDPSTKDELNVVEIEGQDSEGQKVKAVLATLKPSTLPSVCLGGFEITPPVVFRLRTGSGPVHISGQHLVIMGGDQSFDEEEEEEEEEETVMTSKKRPALSTPKPSKKMKMDEEDDDDDDEDDDDEDDDEEDESEEESPVKEKKAPAKPKTPTQNGKGPKPSTPAKQTPQKGKKEQTPKTPQTPRTLADIKSKMMESVAKGVSLPKVQLKFENYVNNCFKGTDPKVVEELWKWRQTVK

Target ORF information:

RefSeq Version	NM_199428.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nucleophosmin 1a (npm1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199428.2

ORF Insert Sequence:

ATGGATCTCG AACAGATGGG TCCCCAAACC TTCCTGTACG GTTGTGAACT GAAAGCAGGC 
AAGGATATCA CGTTTAATCC AGAGGATGAT GATTATGATC ATCAATTATC GGTTAGAATG 
GCCTGTGTGG ATCCCTCGAC AAAAGATGAA TTGAATGTTG TGGAGATTGA AGGACAAGAT 
TCAGAGGGTC AGAAGGTCAA GGCAGTTCTA GCAACACTCA AGCCTTCAAC TCTCCCAAGT 
GTATGTTTGG GTGGGTTTGA GATCACGCCA CCGGTTGTTT TCCGTCTGCG CACTGGTTCT 
GGTCCAGTTC ACATTAGTGG ACAGCATCTT GTTATCATGG GAGGAGATCA GTCTTTTGAT 
GAAGAGGAGG AGGAGGAGGA AGAAGAGGAA ACCGTGATGA CATCCAAGAA GAGGCCAGCG 
CTGTCAACTC CAAAGCCTTC AAAAAAAATG AAGATGGATG AGGAAGATGA CGATGATGAT 
GATGAGGACG ATGATGATGA AGATGACGAT GAGGAAGATG AAAGTGAAGA GGAGTCACCT 
GTGAAAGAAA AGAAAGCACC AGCGAAACCA AAGACCCCTA CACAGAATGG AAAAGGACCC 
AAACCCAGTA CACCTGCTAA ACAAACTCCA CAAAAGGGCA AGAAGGAACA GACACCCAAA 
ACGCCACAAA CACCACGGAC TTTAGCCGAT ATCAAGTCCA AGATGATGGA GAGTGTAGCA 
AAGGGTGTGT CATTACCAAA AGTACAGCTG AAGTTTGAGA ACTATGTGAA TAACTGCTTC 
AAAGGCACAG ATCCAAAGGT TGTTGAGGAG CTCTGGAAGT GGAGACAGAC TGTCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cross talk between TP53 and c-Myc in the pathophysiology of Diamond-Blackfan anemia: Evidence from RPL11-deficient in?vivo and in?vitro models.
Biochemical and biophysical research communications495(2)1839-1845(2018 Jan)
Chakraborty A,Uechi T,Nakajima Y,Gazda HT,O'Donohue MF,Gleizes PE,Kenmochi N

NPM1 directs PIDDosome-dependent caspase-2 activation in the nucleolus.
The Journal of cell biology216(6)1795-1810(2017 Jun)
Ando K,Parsons MJ,Shah RB,Charendoff CI,Paris SL,Liu PH,Fassio SR,Rohrman BA,Thompson R,Oberst A,Sidi S,Bouchier-Hayes L

Maternal methylmercury from a wild-caught walleye diet induces developmental abnormalities in zebrafish.
Reproductive toxicology (Elmsford, N.Y.)65272-282(2016 10)
Liu Q,Klingler RH,Wimpee B,Dellinger M,King-Heiden T,Grzybowski J,Gerstenberger SL,Weber DN,Carvan MJ 3rd

High efficiency In Vivo genome engineering with a simplified 15-RVD GoldyTALEN design.
PloS one8(5)e65259(2013)
Ma AC,Lee HB,Clark KJ,Ekker SC