mst1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	mst1
Entrez Gene ID	259260
Full Name	macrophage stimulating 1
Synonyms	cb981,hgfl1
General protein information	Preferred Names macrophage stimulating 1 Names hepatocyte growth factor-like protein macrophage stimulating 1 (hepatocyte growth factor-like) macrophage-stimulating protein msp
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_152980.1	NP_694512.1	hepatocyte growth factor-like protein precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MST1	NP_001069145.1
Xenopus tropicalis (tropical clawed frog)	mst1	XP_002936531.2
Pan troglodytes (chimpanzee)	MST1	XP_516465.4
Homo sapiens (human)	MST1	NP_066278.3
Canis lupus familiaris (dog)	MST1	XP_541884.3
Gallus gallus (chicken)	MST1	NP_990544.1
	MST1	XP_001107946.1
Rattus norvegicus (Norway rat)	Mst1	NP_077328.1
Danio rerio (zebrafish)	mst1	NP_694512.1
Mus musculus (house mouse)	Mst1	NP_032269.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Efficient identification of CRISPR/Cas9-induced insertions/deletions by direct germline screening in zebrafish.
Brocal I, White RJ, Dooley CM, Carruthers SN, Clark R, Hall A, Busch-Nentwich EM, Stemple DL, Kettleborough RN
BMC genomics17259(2016 Mar)

Deficiency in macrophage-stimulating protein results in spontaneous intestinal inflammation and increased susceptibility toward epithelial damage in zebrafish.
Witte M, Huitema LF, Nieuwenhuis EE, Brugman S
Zebrafish11(6)542-50(2014 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show the Msp/Ron signaling axis as a previously unappreciated player in calcium homeostasis and embryonic skeletal mineralization.
Title: Macrophage-stimulating protein and calcium homeostasis in zebrafish.

The following mst1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mst1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa35905	NM_152980.1 Latest version!	Danio rerio macrophage stimulating 1 (mst1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mst1 ( NM_152980.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_694512.1 Danio rerio macrophage stimulating 1 (mst1), mRNA.

Price & Availability↑

CloneID	ODa35905
Clone ID Related Accession (Same CDS sequence)	NM_152980.1
Accession Version	NM_152980.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Danio rerio(zebrafish)
Product	hepatocyte growth factor-like protein precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF370035.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF370035.1, BC084690.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTTTATGC TCTTCTTTCT CACAGCTCTC CTGCTTCGAC TCGATTCAGT TGATGGATAC 
CGCAGCGCAC TAAATGATTT TCAGCGGTCG GAGGGAAGAG AACTGGTTCT GAAATCCTGG 
AACGTGAACC GACTAACTGA ACTTCCGCGA GTCACACTGG AGGATTGCGC TAAACGCTGC 
ACAGAGAGTC CAGAATGCAG AGCTTTCAAC TATGAGTTTC GTCCATCATT GGTGTGTAAA 
CATCTGCCCT GGGTGGGCGA CGGAGACAAT GCAGACGTGA AGAGAAACGT GAACTGTGAC 
CTCTATGAGA TGAAGGTATA TGTAAGGAAA TGCATTGTGG GTAAAGGAGA AGACTATCGA 
GGGAAAGTGT CGACAACTAC AAGTGGACGA ACATGTCAAC AGTGGTGGTC AAAATTCCCT 
CATGACCACA GATGGACTCC GTCTGCGACT AATGGCCTGG AACTGAACTA CTGCCGAAAT 
CCAGACGGAG ACCGCATTGG CCCCTGGTGT TACACCACTG ACCCTGAACG CAGATACGAG 
AGTTGCAACA TCCCTCAGTG CAAAGACGAG GTGTGCATCA CTTGTAATGG TGAAGACTAC 
AGAGGGCAAG TGGATCACAC TATCAGCGGA AAAGAGTGTC AGAGATGGGA CCAACAGTTT 
CCACATCAAC ACATCTACCA GCCGGAGAAA TATCCGGATA AAAGTTTGGA TGATAATTAT 
TGTCGCAACC CGGATGCGTC TCCGGTGCCC TGGTGTTACA CCACTGACCC AACAATGGAG 
AGAGAGAGCT GTGATATAAG CAAGTGCCCT GAATTTCCCA AGCGTCGTCT TCGCTCCAGC 
TACACCACTA ACTGCTTTCG GGGGCGAGGC GAGGATTACA GGGGCAAAGT CAACGAAACA 
ACCTCTGGAA TCCCTTGTCA GCGTTGGGAT GCCCAGAAAC CTCACGAACA CCCATTTTTC 
CCCAAAACCT ACGAGTGCAA GGGTTTAGAG GAGAACTACT GTCGTAACCC GGACGGCTCA 
GAAGCACCGT GGTGTTTTAC GTCTCTGCCG GAGATGCGGA CGGCGCTCTG CCTGCAGATC 
AAACGCTGCG CTGATGACAT CGAGGCTGAA GATTGCTATA ATGAAATCGG CAAAAACTAT 
CGAGGTGTGG TCCGCAAGAC CCGGAAAGGA ATCCTCTGCC AGAAATGGAG CATCAACACA 
CCTCACAAAA CCAAGATAAA CCCCAAAACA CACCCAGAAG CAAACCTTAC GGACAACTAC 
TGCAGGAATC CAGACGGAGA TCATCACGGC CCCTGGTGCT ACACCTCTGA CCCCAAGACT 
GAGTTTGACT ACTGCGCCAT CAAGCAGTGC GCTGGAGAGA AAGTGCCAAT CATCGGTCCA 
ACAACCGAGG TTGAGTTTAA TGAGTGCGGA AAGCGTGAAG ACCGGCTGAG GTCCAGGTTA 
AGAATCGTGG GCGGCACACC TGGAAACTCC CCCTGGACCG TCAGCCTCAG AGATCGGAAA 
GGGAATCACT TCTGTGGAGG CTCTCTGGTC AGTTCTGAAT GGGTCATCAG CACTAAACAG 
TGCTTCTCAT CCTGTTATGT GGACCTGACG GGATACACTG CTATGATGGG CACCTTGTTT 
CGTGACCCAA AAGAGGGAGA ACCAGACCTA CAGAGGATTT CTCTCACCAA AATCGTCTGC 
GGCCCTTCTG AATCCCACCT GGTCATGCTG CAGCTGGAGA CTCCTGCTCA GTTTAATGAG 
CGCGTGTCCC AGATCTGCCT TCCTCCCGAG CGCTACATCG TTCCTGATGG GACCATCTGT 
GAAATTGCTG GATGGGGAGA AACTAAAGGA AAGGGAGATG AAACGGTGCT GAATGTGGCT 
CAGATGCCGG TGCTCAGCAA TAAGGACTGT AACCAGTACT TCAAGGGCCG CGTGCGTGAG 
AATGAGATGT GCACCATGGC CTTCCAGGCT GGAGTGGGAG CCTGTGAGAG GGATTACGGA 
GGCCCGCTGG CCTGTCAGAA CAGTGACTGC TGGGTCCTAG AGGGAGTCAT TATTCCCATG 
CGCCGCTGTG GACACGCAGG ACAACCCAAC ATCTTTATCC GCGTCTCGGT TTATGTGGAC 
TGGATAAAGA AAGTCATGGA GATGACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_694512.1
CDS	50..2179
Translation	MFMLFFLTALLLRLDSVDGYRSALNDFQRSEGRELVLKSWNVNRLTELPRVTLEDCAKRCTESPECRAFNYEFRPSLVCKHLPWVGDGDNADVKRNVNCDLYEMKVYVRKCIVGKGEDYRGKVSTTTSGRTCQQWWSKFPHDHRWTPSATNGLELNYCRNPDGDRIGPWCYTTDPERRYESCNIPQCKDEVCITCNGEDYRGQVDHTISGKECQRWDQQFPHQHIYQPEKYPDKSLDDNYCRNPDASPVPWCYTTDPTMERESCDISKCPEFPKRRLRSSYTTNCFRGRGEDYRGKVNETTSGIPCQRWDAQKPHEHPFFPKTYECKGLEENYCRNPDGSEAPWCFTSLPEMRTALCLQIKRCADDIEAEDCYNEIGKNYRGVVRKTRKGILCQKWSINTPHKTKINPKTHPEANLTDNYCRNPDGDHHGPWCYTSDPKTEFDYCAIKQCAGEKVPIIGPTTEVEFNECGKREDRLRSRLRIVGGTPGNSPWTVSLRDRKGNHFCGGSLVSSEWVISTKQCFSSCYVDLTGYTAMMGTLFRDPKEGEPDLQRISLTKIVCGPSESHLVMLQLETPAQFNERVSQICLPPERYIVPDGTICEIAGWGETKGKGDETVLNVAQMPVLSNKDCNQYFKGRVRENEMCTMAFQAGVGACERDYGGPLACQNSDCWVLEGVIIPMRRCGHAGQPNIFIRVSVYVDWIKKVMEMT

Target ORF information:

RefSeq Version	NM_152980.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio macrophage stimulating 1 (mst1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152980.1

ORF Insert Sequence:

ATGTTTATGC TCTTCTTTCT CACAGCTCTC CTGCTTCGAC TCGATTCAGT TGATGGATAC 
CGCAGCGCAC TAAATGATTT TCAGCGGTCG GAGGGAAGAG AACTGGTTCT GAAATCCTGG 
AACGTGAACC GACTAACTGA ACTTCCGCGA GTCACACTGG AGGATTGCGC TAAACGCTGC 
ACAGAGAGTC CAGAATGCAG AGCTTTCAAC TATGAGTTTC GTCCATCATT GGTGTGTAAA 
CATCTGCCCT GGGTGGGCGA CGGAGACAAT GCAGACGTGA AGAGAAACGT GAACTGTGAC 
CTCTATGAGA TGAAGGTATA TGTAAGGAAA TGCATTGTGG GTAAAGGAGA AGACTATCGA 
GGGAAAGTGT CGACAACTAC AAGTGGACGA ACATGTCAAC AGTGGTGGTC AAAATTCCCT 
CATGACCACA GATGGACTCC GTCTGCGACT AATGGCCTGG AACTGAACTA CTGCCGAAAT 
CCAGACGGAG ACCGCATTGG CCCCTGGTGT TACACCACTG ACCCTGAACG CAGATACGAG 
AGTTGCAACA TCCCTCAGTG CAAAGACGAG GTGTGCATCA CTTGTAATGG TGAAGACTAC 
AGAGGGCAAG TGGATCACAC TATCAGCGGA AAAGAGTGTC AGAGATGGGA CCAACAGTTT 
CCACATCAAC ACATCTACCA GCCGGAGAAA TATCCGGATA AAAGTTTGGA TGATAATTAT 
TGTCGCAACC CGGATGCGTC TCCGGTGCCC TGGTGTTACA CCACTGACCC AACAATGGAG 
AGAGAGAGCT GTGATATAAG CAAGTGCCCT GAATTTCCCA AGCGTCGTCT TCGCTCCAGC 
TACACCACTA ACTGCTTTCG GGGGCGAGGC GAGGATTACA GGGGCAAAGT CAACGAAACA 
ACCTCTGGAA TCCCTTGTCA GCGTTGGGAT GCCCAGAAAC CTCACGAACA CCCATTTTTC 
CCCAAAACCT ACGAGTGCAA GGGTTTAGAG GAGAACTACT GTCGTAACCC GGACGGCTCA 
GAAGCACCGT GGTGTTTTAC GTCTCTGCCG GAGATGCGGA CGGCGCTCTG CCTGCAGATC 
AAACGCTGCG CTGATGACAT CGAGGCTGAA GATTGCTATA ATGAAATCGG CAAAAACTAT 
CGAGGTGTGG TCCGCAAGAC CCGGAAAGGA ATCCTCTGCC AGAAATGGAG CATCAACACA 
CCTCACAAAA CCAAGATAAA CCCCAAAACA CACCCAGAAG CAAACCTTAC GGACAACTAC 
TGCAGGAATC CAGACGGAGA TCATCACGGC CCCTGGTGCT ACACCTCTGA CCCCAAGACT 
GAGTTTGACT ACTGCGCCAT CAAGCAGTGC GCTGGAGAGA AAGTGCCAAT CATCGGTCCA 
ACAACCGAGG TTGAGTTTAA TGAGTGCGGA AAGCGTGAAG ACCGGCTGAG GTCCAGGTTA 
AGAATCGTGG GCGGCACACC TGGAAACTCC CCCTGGACCG TCAGCCTCAG AGATCGGAAA 
GGGAATCACT TCTGTGGAGG CTCTCTGGTC AGTTCTGAAT GGGTCATCAG CACTAAACAG 
TGCTTCTCAT CCTGTTATGT GGACCTGACG GGATACACTG CTATGATGGG CACCTTGTTT 
CGTGACCCAA AAGAGGGAGA ACCAGACCTA CAGAGGATTT CTCTCACCAA AATCGTCTGC 
GGCCCTTCTG AATCCCACCT GGTCATGCTG CAGCTGGAGA CTCCTGCTCA GTTTAATGAG 
CGCGTGTCCC AGATCTGCCT TCCTCCCGAG CGCTACATCG TTCCTGATGG GACCATCTGT 
GAAATTGCTG GATGGGGAGA AACTAAAGGA AAGGGAGATG AAACGGTGCT GAATGTGGCT 
CAGATGCCGG TGCTCAGCAA TAAGGACTGT AACCAGTACT TCAAGGGCCG CGTGCGTGAG 
AATGAGATGT GCACCATGGC CTTCCAGGCT GGAGTGGGAG CCTGTGAGAG GGATTACGGA 
GGCCCGCTGG CCTGTCAGAA CAGTGACTGC TGGGTCCTAG AGGGAGTCAT TATTCCCATG 
CGCCGCTGTG GACACGCAGG ACAACCCAAC ATCTTTATCC GCGTCTCGGT TTATGTGGAC 
TGGATAAAGA AAGTCATGGA GATGACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Efficient identification of CRISPR/Cas9-induced insertions/deletions by direct germline screening in zebrafish.
BMC genomics17259(2016 Mar)
Brocal I,White RJ,Dooley CM,Carruthers SN,Clark R,Hall A,Busch-Nentwich EM,Stemple DL,Kettleborough RN

Deficiency in macrophage-stimulating protein results in spontaneous intestinal inflammation and increased susceptibility toward epithelial damage in zebrafish.
Zebrafish11(6)542-50(2014 Dec)
Witte M,Huitema LF,Nieuwenhuis EE,Brugman S

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mst1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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