mkrn2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	mkrn2
Entrez Gene ID	170783
Full Name	makorin, ring finger protein, 2
Synonyms	hspc070,wu:fb99a04
General protein information	Preferred Names makorin, ring finger protein, 2 Names probable E3 ubiquitin-protein ligase makorin-2 RING-type E3 ubiquitin transferase makorin-2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005155668.3	XP_005155725.1	probable E3 ubiquitin-protein ligase makorin-2 isoform X1
NM_152979.2	NP_694511.1	probable E3 ubiquitin-protein ligase makorin-2
XM_005155668.4	XP_005155725.1	probable E3 ubiquitin-protein ligase makorin-2 isoform X1

Pathways from BioSystems

Homologies

	MKRN2	XP_001086204.2
Canis lupus familiaris (dog)	MKRN2	XP_005632195.1
Arabidopsis thaliana (thale cress)	AT3G08505	NP_974255.1
Pan troglodytes (chimpanzee)	MKRN2	XP_516287.3
Gallus gallus (chicken)	MKRN2	NP_989619.1
Oryza sativa (rice)	Os06g0318700	NP_001057505.1
Homo sapiens (human)	MKRN2	NP_054879.3
Bos taurus (cattle)	MKRN2	NP_001039454.1
Mus musculus (house mouse)	Mkrn2	NP_075779.2
Rattus norvegicus (Norway rat)	Mkrn2	NP_001008315.1
Danio rerio (zebrafish)	mkrn2	NP_694511.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Differential expression of synapsin genes during early zebrafish development.
Garbarino G, Costa S, Pestarino M, Candiani S
Neuroscience280351-67(2014 Nov)

The vertebrate makorin ubiquitin ligase gene family has been shaped by large-scale duplication and retroposition from an ancestral gonad-specific, maternal-effect gene.
B?hne A, Darras A, D'Cotta H, Baroiller JF, Galiana-Arnoux D, Volff JN
BMC genomics11721(2010 Dec)

The zebrafish gene map defines ancestral vertebrate chromosomes.
Woods IG, Wilson C, Friedlander B, Chang P, Reyes DK, Nix R, Kelly PD, Chu F, Postlethwait JH, Talbot WS
Genome research15(9)1307-14(2005 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mkrn2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mkrn2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa16589	NM_152979.2 Latest version!	Danio rerio makorin, ring finger protein, 2 (mkrn2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa16589	XM_005155668.4 Latest version!	Danio rerio makorin, ring finger protein, 2 (mkrn2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
View Old Accession Versions >>
ODa16589	XM_005155668.3	Danio rerio makorin, ring finger protein, 2 (mkrn2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mkrn2 ( NM_152979.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_694511.1 Danio rerio makorin, ring finger protein, 2 (mkrn2), mRNA.

Price & Availability↑

CloneID	ODa16589
Clone ID Related Accession (Same CDS sequence)	XM_005155668.3 , NM_152979.2 , XM_005155668.4
Accession Version	NM_152979.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Danio rerio(zebrafish)
Product	probable E3 ubiquitin-protein ligase makorin-2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CABZ01008492.1, CN321915.1, BC065352.1, CABZ01008493.1 and AI588295.1. On or before Feb 11, 2016 this sequence version replaced XM_005155667.2, NM_152979.1. ##Evidence-Data-START## Transcript exon combination :: AF277172.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTCAACGA AACAAGTGAC GTGCAGGTAT TTCCTCCACG GTGTGTGTCG GGAGGGCAGC 
AGGTGTCTGT TTTCTCACGA TTTAACCACA AGTAAACCTT CCACCATCTG CAAATACTAC 
CAGAGAGGAG CGTGTGCTTA TGGAGACAGA TGCAGATATG ACCATATTAA ACCTCCTGGT 
CGTGGCAGTG GAGCTCCAGC AGATCACAGT AACAGAAGCA GCAGCAGTGC AGGAGCCTCC 
GCACCCGGCC CCGGACCCCC GGCAAACACC AGCAAACACC TCAAAAAACC ACTAGTGCTT 
CGAGACAAAG CTCTGTGCTC AGACAGCAGA CCTCGTGTGT TCAGTGCGGA GAGTTCAGAG 
CTCAGTGAAT GCTGGGAGCA GCGAGACGAC GGCGCTCAGA AGCCGCACTC GTATCTGGAG 
GCCATCCGCT CTGGCCTGGA CGCCTCCGCC GCCGCCGCTG CCACCGCTGG GACGTTTCCA 
GAGCTCCAGC AGACGTCGCC GCAGATCTGC CCGTTCCTGG CCGCAGGGCA GTGCCAGTAT 
GGAGAAAGCT GTCCGTATCT GCATGGGGAG ATGTGTGAGA TCTGCAGACA GCATGTGCTG 
CATCCACACG ACCCCGAGCA GAGAGCAGCG CACGAGAAGA AGTGTATGGT GGCGTTTGAG 
ATGGACATGG AGCGAGCGTT CGCAGTGCAG CAGAGTCAGG ATAAGGTGTG TAAGATCTGT 
CTGGATGTGG TGTACGAGAA ATCCTCTCCG TCTGAGCGGC GCTTCGGCAT CCTGTCCAGC 
TGCGCACACA CATACTGCCT GAACTGCATC CGCCAGTGGC GCTGCGTAGA GCAGTTACAC 
AACCAGATAC GCAAGTCTTG TCCAGAGTGT CGTGTGGTCT CAGAGTTCGT GATTCCCAGC 
ATCTACTGGG TGGAGGATCA GGAGCAGAAA AACCTGCTCA TCGAGGAGTT CAAGTCTGGA 
GTCAGTAAGA AAGCCTGTAA GTACTTTGAC CAGGGCAGAG GAACGTGTCC GTTTGGAGGG 
AAGTGTTTCT ACATGCACGC TTATGCTGAC GGCAGACGAG CAGAACCTGA CAAACCCCGA 
AAACAGCTCA GCGCTGAGGG AAACGTGCGG TTCCAGAACT CGGTGCGTCT GTGGGACTTC 
ATCGAGGAGC GCGAGCACCG GAGTGTCCCG CAGCTGGAGG ACGAGGTCAA TGACCTGGGG 
GAACTCTTCA TGCAGCTGTC AGGAGCCTCA GACGCACCGC ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_694511.1
CDS	113..1357
Translation	MSTKQVTCRYFLHGVCREGSRCLFSHDLTTSKPSTICKYYQRGACAYGDRCRYDHIKPPGRGSGAPADHSNRSSSSAGASAPGPGPPANTSKHLKKPLVLRDKALCSDSRPRVFSAESSELSECWEQRDDGAQKPHSYLEAIRSGLDASAAAAATAGTFPELQQTSPQICPFLAAGQCQYGESCPYLHGEMCEICRQHVLHPHDPEQRAAHEKKCMVAFEMDMERAFAVQQSQDKVCKICLDVVYEKSSPSERRFGILSSCAHTYCLNCIRQWRCVEQLHNQIRKSCPECRVVSEFVIPSIYWVEDQEQKNLLIEEFKSGVSKKACKYFDQGRGTCPFGGKCFYMHAYADGRRAEPDKPRKQLSAEGNVRFQNSVRLWDFIEEREHRSVPQLEDEVNDLGELFMQLSGASDAPH

Target ORF information:

RefSeq Version	NM_152979.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio makorin, ring finger protein, 2 (mkrn2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152979.2

ORF Insert Sequence:

ATGTCAACGA AACAAGTGAC GTGCAGGTAT TTCCTCCACG GTGTGTGTCG GGAGGGCAGC 
AGGTGTCTGT TTTCTCACGA TTTAACCACA AGTAAACCTT CCACCATCTG CAAATACTAC 
CAGAGAGGAG CGTGTGCTTA TGGAGACAGA TGCAGATATG ACCATATTAA ACCTCCTGGT 
CGTGGCAGTG GAGCTCCAGC AGATCACAGT AACAGAAGCA GCAGCAGTGC AGGAGCCTCC 
GCACCCGGCC CCGGACCCCC GGCAAACACC AGCAAACACC TCAAAAAACC ACTAGTGCTT 
CGAGACAAAG CTCTGTGCTC AGACAGCAGA CCTCGTGTGT TCAGTGCGGA GAGTTCAGAG 
CTCAGTGAAT GCTGGGAGCA GCGAGACGAC GGCGCTCAGA AGCCGCACTC GTATCTGGAG 
GCCATCCGCT CTGGCCTGGA CGCCTCCGCC GCCGCCGCTG CCACCGCTGG GACGTTTCCA 
GAGCTCCAGC AGACGTCGCC GCAGATCTGC CCGTTCCTGG CCGCAGGGCA GTGCCAGTAT 
GGAGAAAGCT GTCCGTATCT GCATGGGGAG ATGTGTGAGA TCTGCAGACA GCATGTGCTG 
CATCCACACG ACCCCGAGCA GAGAGCAGCG CACGAGAAGA AGTGTATGGT GGCGTTTGAG 
ATGGACATGG AGCGAGCGTT CGCAGTGCAG CAGAGTCAGG ATAAGGTGTG TAAGATCTGT 
CTGGATGTGG TGTACGAGAA ATCCTCTCCG TCTGAGCGGC GCTTCGGCAT CCTGTCCAGC 
TGCGCACACA CATACTGCCT GAACTGCATC CGCCAGTGGC GCTGCGTAGA GCAGTTACAC 
AACCAGATAC GCAAGTCTTG TCCAGAGTGT CGTGTGGTCT CAGAGTTCGT GATTCCCAGC 
ATCTACTGGG TGGAGGATCA GGAGCAGAAA AACCTGCTCA TCGAGGAGTT CAAGTCTGGA 
GTCAGTAAGA AAGCCTGTAA GTACTTTGAC CAGGGCAGAG GAACGTGTCC GTTTGGAGGG 
AAGTGTTTCT ACATGCACGC TTATGCTGAC GGCAGACGAG CAGAACCTGA CAAACCCCGA 
AAACAGCTCA GCGCTGAGGG AAACGTGCGG TTCCAGAACT CGGTGCGTCT GTGGGACTTC 
ATCGAGGAGC GCGAGCACCG GAGTGTCCCG CAGCTGGAGG ACGAGGTCAA TGACCTGGGG 
GAACTCTTCA TGCAGCTGTC AGGAGCCTCA GACGCACCGC ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mkrn2 ( XM_005155668.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155725.1 Danio rerio makorin, ring finger protein, 2 (mkrn2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa16589
Clone ID Related Accession (Same CDS sequence)	XM_005155668.3 , NM_152979.2 , XM_005155668.4
Accession Version	XM_005155668.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	probable E3 ubiquitin-protein ligase makorin-2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005155668.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAACGA AACAAGTGAC GTGCAGGTAT TTCCTCCACG GTGTGTGTCG GGAGGGCAGC 
AGGTGTCTGT TTTCTCACGA TTTAACCACA AGTAAACCTT CCACCATCTG CAAATACTAC 
CAGAGAGGAG CGTGTGCTTA TGGAGACAGA TGCAGATATG ACCATATTAA ACCTCCTGGT 
CGTGGCAGTG GAGCTCCAGC AGATCACAGT AACAGAAGCA GCAGCAGTGC AGGAGCCTCC 
GCACCCGGCC CCGGACCCCC GGCAAACACC AGCAAACACC TCAAAAAACC ACTAGTGCTT 
CGAGACAAAG CTCTGTGCTC AGACAGCAGA CCTCGTGTGT TCAGTGCGGA GAGTTCAGAG 
CTCAGTGAAT GCTGGGAGCA GCGAGACGAC GGCGCTCAGA AGCCGCACTC GTATCTGGAG 
GCCATCCGCT CTGGCCTGGA CGCCTCCGCC GCCGCCGCTG CCACCGCTGG GACGTTTCCA 
GAGCTCCAGC AGACGTCGCC GCAGATCTGC CCGTTCCTGG CCGCAGGGCA GTGCCAGTAT 
GGAGAAAGCT GTCCGTATCT GCATGGGGAG ATGTGTGAGA TCTGCAGACA GCATGTGCTG 
CATCCACACG ACCCCGAGCA GAGAGCAGCG CACGAGAAGA AGTGTATGGT GGCGTTTGAG 
ATGGACATGG AGCGAGCGTT CGCAGTGCAG CAGAGTCAGG ATAAGGTGTG TAAGATCTGT 
CTGGATGTGG TGTACGAGAA ATCCTCTCCG TCTGAGCGGC GCTTCGGCAT CCTGTCCAGC 
TGCGCACACA CATACTGCCT GAACTGCATC CGCCAGTGGC GCTGCGTAGA GCAGTTACAC 
AACCAGATAC GCAAGTCTTG TCCAGAGTGT CGTGTGGTCT CAGAGTTCGT GATTCCCAGC 
ATCTACTGGG TGGAGGATCA GGAGCAGAAA AACCTGCTCA TCGAGGAGTT CAAGTCTGGA 
GTCAGTAAGA AAGCCTGTAA GTACTTTGAC CAGGGCAGAG GAACGTGTCC GTTTGGAGGG 
AAGTGTTTCT ACATGCACGC TTATGCTGAC GGCAGACGAG CAGAACCTGA CAAACCCCGA 
AAACAGCTCA GCGCTGAGGG AAACGTGCGG TTCCAGAACT CGGTGCGTCT GTGGGACTTC 
ATCGAGGAGC GCGAGCACCG GAGTGTCCCG CAGCTGGAGG ACGAGGTCAA TGACCTGGGG 
GAACTCTTCA TGCAGCTGTC AGGAGCCTCA GACGCACCGC ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155725.1
CDS	123..1367
Translation	MSTKQVTCRYFLHGVCREGSRCLFSHDLTTSKPSTICKYYQRGACAYGDRCRYDHIKPPGRGSGAPADHSNRSSSSAGASAPGPGPPANTSKHLKKPLVLRDKALCSDSRPRVFSAESSELSECWEQRDDGAQKPHSYLEAIRSGLDASAAAAATAGTFPELQQTSPQICPFLAAGQCQYGESCPYLHGEMCEICRQHVLHPHDPEQRAAHEKKCMVAFEMDMERAFAVQQSQDKVCKICLDVVYEKSSPSERRFGILSSCAHTYCLNCIRQWRCVEQLHNQIRKSCPECRVVSEFVIPSIYWVEDQEQKNLLIEEFKSGVSKKACKYFDQGRGTCPFGGKCFYMHAYADGRRAEPDKPRKQLSAEGNVRFQNSVRLWDFIEEREHRSVPQLEDEVNDLGELFMQLSGASDAPH

Target ORF information:

RefSeq Version	XM_005155668.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio makorin, ring finger protein, 2 (mkrn2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155668.3

ORF Insert Sequence:

ATGTCAACGA AACAAGTGAC GTGCAGGTAT TTCCTCCACG GTGTGTGTCG GGAGGGCAGC 
AGGTGTCTGT TTTCTCACGA TTTAACCACA AGTAAACCTT CCACCATCTG CAAATACTAC 
CAGAGAGGAG CGTGTGCTTA TGGAGACAGA TGCAGATATG ACCATATTAA ACCTCCTGGT 
CGTGGCAGTG GAGCTCCAGC AGATCACAGT AACAGAAGCA GCAGCAGTGC AGGAGCCTCC 
GCACCCGGCC CCGGACCCCC GGCAAACACC AGCAAACACC TCAAAAAACC ACTAGTGCTT 
CGAGACAAAG CTCTGTGCTC AGACAGCAGA CCTCGTGTGT TCAGTGCGGA GAGTTCAGAG 
CTCAGTGAAT GCTGGGAGCA GCGAGACGAC GGCGCTCAGA AGCCGCACTC GTATCTGGAG 
GCCATCCGCT CTGGCCTGGA CGCCTCCGCC GCCGCCGCTG CCACCGCTGG GACGTTTCCA 
GAGCTCCAGC AGACGTCGCC GCAGATCTGC CCGTTCCTGG CCGCAGGGCA GTGCCAGTAT 
GGAGAAAGCT GTCCGTATCT GCATGGGGAG ATGTGTGAGA TCTGCAGACA GCATGTGCTG 
CATCCACACG ACCCCGAGCA GAGAGCAGCG CACGAGAAGA AGTGTATGGT GGCGTTTGAG 
ATGGACATGG AGCGAGCGTT CGCAGTGCAG CAGAGTCAGG ATAAGGTGTG TAAGATCTGT 
CTGGATGTGG TGTACGAGAA ATCCTCTCCG TCTGAGCGGC GCTTCGGCAT CCTGTCCAGC 
TGCGCACACA CATACTGCCT GAACTGCATC CGCCAGTGGC GCTGCGTAGA GCAGTTACAC 
AACCAGATAC GCAAGTCTTG TCCAGAGTGT CGTGTGGTCT CAGAGTTCGT GATTCCCAGC 
ATCTACTGGG TGGAGGATCA GGAGCAGAAA AACCTGCTCA TCGAGGAGTT CAAGTCTGGA 
GTCAGTAAGA AAGCCTGTAA GTACTTTGAC CAGGGCAGAG GAACGTGTCC GTTTGGAGGG 
AAGTGTTTCT ACATGCACGC TTATGCTGAC GGCAGACGAG CAGAACCTGA CAAACCCCGA 
AAACAGCTCA GCGCTGAGGG AAACGTGCGG TTCCAGAACT CGGTGCGTCT GTGGGACTTC 
ATCGAGGAGC GCGAGCACCG GAGTGTCCCG CAGCTGGAGG ACGAGGTCAA TGACCTGGGG 
GAACTCTTCA TGCAGCTGTC AGGAGCCTCA GACGCACCGC ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mkrn2 ( XM_005155668.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155725.1 Danio rerio makorin, ring finger protein, 2 (mkrn2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa16589
Clone ID Related Accession (Same CDS sequence)	XM_005155668.3 , NM_152979.2 , XM_005155668.4
Accession Version	XM_005155668.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	probable E3 ubiquitin-protein ligase makorin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005155668.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAACGA AACAAGTGAC GTGCAGGTAT TTCCTCCACG GTGTGTGTCG GGAGGGCAGC 
AGGTGTCTGT TTTCTCACGA TTTAACCACA AGTAAACCTT CCACCATCTG CAAATACTAC 
CAGAGAGGAG CGTGTGCTTA TGGAGACAGA TGCAGATATG ACCATATTAA ACCTCCTGGT 
CGTGGCAGTG GAGCTCCAGC AGATCACAGT AACAGAAGCA GCAGCAGTGC AGGAGCCTCC 
GCACCCGGCC CCGGACCCCC GGCAAACACC AGCAAACACC TCAAAAAACC ACTAGTGCTT 
CGAGACAAAG CTCTGTGCTC AGACAGCAGA CCTCGTGTGT TCAGTGCGGA GAGTTCAGAG 
CTCAGTGAAT GCTGGGAGCA GCGAGACGAC GGCGCTCAGA AGCCGCACTC GTATCTGGAG 
GCCATCCGCT CTGGCCTGGA CGCCTCCGCC GCCGCCGCTG CCACCGCTGG GACGTTTCCA 
GAGCTCCAGC AGACGTCGCC GCAGATCTGC CCGTTCCTGG CCGCAGGGCA GTGCCAGTAT 
GGAGAAAGCT GTCCGTATCT GCATGGGGAG ATGTGTGAGA TCTGCAGACA GCATGTGCTG 
CATCCACACG ACCCCGAGCA GAGAGCAGCG CACGAGAAGA AGTGTATGGT GGCGTTTGAG 
ATGGACATGG AGCGAGCGTT CGCAGTGCAG CAGAGTCAGG ATAAGGTGTG TAAGATCTGT 
CTGGATGTGG TGTACGAGAA ATCCTCTCCG TCTGAGCGGC GCTTCGGCAT CCTGTCCAGC 
TGCGCACACA CATACTGCCT GAACTGCATC CGCCAGTGGC GCTGCGTAGA GCAGTTACAC 
AACCAGATAC GCAAGTCTTG TCCAGAGTGT CGTGTGGTCT CAGAGTTCGT GATTCCCAGC 
ATCTACTGGG TGGAGGATCA GGAGCAGAAA AACCTGCTCA TCGAGGAGTT CAAGTCTGGA 
GTCAGTAAGA AAGCCTGTAA GTACTTTGAC CAGGGCAGAG GAACGTGTCC GTTTGGAGGG 
AAGTGTTTCT ACATGCACGC TTATGCTGAC GGCAGACGAG CAGAACCTGA CAAACCCCGA 
AAACAGCTCA GCGCTGAGGG AAACGTGCGG TTCCAGAACT CGGTGCGTCT GTGGGACTTC 
ATCGAGGAGC GCGAGCACCG GAGTGTCCCG CAGCTGGAGG ACGAGGTCAA TGACCTGGGG 
GAACTCTTCA TGCAGCTGTC AGGAGCCTCA GACGCACCGC ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155725.1
CDS	131..1375
Translation	MSTKQVTCRYFLHGVCREGSRCLFSHDLTTSKPSTICKYYQRGACAYGDRCRYDHIKPPGRGSGAPADHSNRSSSSAGASAPGPGPPANTSKHLKKPLVLRDKALCSDSRPRVFSAESSELSECWEQRDDGAQKPHSYLEAIRSGLDASAAAAATAGTFPELQQTSPQICPFLAAGQCQYGESCPYLHGEMCEICRQHVLHPHDPEQRAAHEKKCMVAFEMDMERAFAVQQSQDKVCKICLDVVYEKSSPSERRFGILSSCAHTYCLNCIRQWRCVEQLHNQIRKSCPECRVVSEFVIPSIYWVEDQEQKNLLIEEFKSGVSKKACKYFDQGRGTCPFGGKCFYMHAYADGRRAEPDKPRKQLSAEGNVRFQNSVRLWDFIEEREHRSVPQLEDEVNDLGELFMQLSGASDAPH

Target ORF information:

RefSeq Version	XM_005155668.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio makorin, ring finger protein, 2 (mkrn2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155668.4

ORF Insert Sequence:

ATGTCAACGA AACAAGTGAC GTGCAGGTAT TTCCTCCACG GTGTGTGTCG GGAGGGCAGC 
AGGTGTCTGT TTTCTCACGA TTTAACCACA AGTAAACCTT CCACCATCTG CAAATACTAC 
CAGAGAGGAG CGTGTGCTTA TGGAGACAGA TGCAGATATG ACCATATTAA ACCTCCTGGT 
CGTGGCAGTG GAGCTCCAGC AGATCACAGT AACAGAAGCA GCAGCAGTGC AGGAGCCTCC 
GCACCCGGCC CCGGACCCCC GGCAAACACC AGCAAACACC TCAAAAAACC ACTAGTGCTT 
CGAGACAAAG CTCTGTGCTC AGACAGCAGA CCTCGTGTGT TCAGTGCGGA GAGTTCAGAG 
CTCAGTGAAT GCTGGGAGCA GCGAGACGAC GGCGCTCAGA AGCCGCACTC GTATCTGGAG 
GCCATCCGCT CTGGCCTGGA CGCCTCCGCC GCCGCCGCTG CCACCGCTGG GACGTTTCCA 
GAGCTCCAGC AGACGTCGCC GCAGATCTGC CCGTTCCTGG CCGCAGGGCA GTGCCAGTAT 
GGAGAAAGCT GTCCGTATCT GCATGGGGAG ATGTGTGAGA TCTGCAGACA GCATGTGCTG 
CATCCACACG ACCCCGAGCA GAGAGCAGCG CACGAGAAGA AGTGTATGGT GGCGTTTGAG 
ATGGACATGG AGCGAGCGTT CGCAGTGCAG CAGAGTCAGG ATAAGGTGTG TAAGATCTGT 
CTGGATGTGG TGTACGAGAA ATCCTCTCCG TCTGAGCGGC GCTTCGGCAT CCTGTCCAGC 
TGCGCACACA CATACTGCCT GAACTGCATC CGCCAGTGGC GCTGCGTAGA GCAGTTACAC 
AACCAGATAC GCAAGTCTTG TCCAGAGTGT CGTGTGGTCT CAGAGTTCGT GATTCCCAGC 
ATCTACTGGG TGGAGGATCA GGAGCAGAAA AACCTGCTCA TCGAGGAGTT CAAGTCTGGA 
GTCAGTAAGA AAGCCTGTAA GTACTTTGAC CAGGGCAGAG GAACGTGTCC GTTTGGAGGG 
AAGTGTTTCT ACATGCACGC TTATGCTGAC GGCAGACGAG CAGAACCTGA CAAACCCCGA 
AAACAGCTCA GCGCTGAGGG AAACGTGCGG TTCCAGAACT CGGTGCGTCT GTGGGACTTC 
ATCGAGGAGC GCGAGCACCG GAGTGTCCCG CAGCTGGAGG ACGAGGTCAA TGACCTGGGG 
GAACTCTTCA TGCAGCTGTC AGGAGCCTCA GACGCACCGC ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Differential expression of synapsin genes during early zebrafish development.
Neuroscience280351-67(2014 Nov)
Garbarino G,Costa S,Pestarino M,Candiani S

The vertebrate makorin ubiquitin ligase gene family has been shaped by large-scale duplication and retroposition from an ancestral gonad-specific, maternal-effect gene.
BMC genomics11721(2010 Dec)
B?hne A,Darras A,D'Cotta H,Baroiller JF,Galiana-Arnoux D,Volff JN

The zebrafish gene map defines ancestral vertebrate chromosomes.
Genome research15(9)1307-14(2005 Sep)
Woods IG,Wilson C,Friedlander B,Chang P,Reyes DK,Nix R,Kelly PD,Chu F,Postlethwait JH,Talbot WS

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mkrn2 cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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