MTCL1 cDNA ORF clone, Danio rerio(Zebrafish)

The following MTCL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MTCL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa56507 XM_005171457.3
Latest version!
Danio rerio microtubule crosslinking factor 1 (mtcl1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa56508 XM_017352451.2
Latest version!
Danio rerio microtubule crosslinking factor 1 (mtcl1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa56509 XM_017352452.2
Latest version!
Danio rerio microtubule crosslinking factor 1 (mtcl1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa56510 XM_017352453.2
Latest version!
Danio rerio microtubule crosslinking factor 1 (mtcl1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa56511 XM_017352457.2
Latest version!
Danio rerio microtubule crosslinking factor 1 (mtcl1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
View Old Accession Versions >>
ODa56511 XM_017352457.1 Danio rerio microtubule crosslinking factor 1 (mtcl1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa56508 XM_017352451.1 Danio rerio microtubule crosslinking factor 1 (mtcl1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa56507 XM_005171457.2 Danio rerio microtubule crosslinking factor 1 (mtcl1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa56509 XM_017352452.1 Danio rerio microtubule crosslinking factor 1 (mtcl1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa56510 XM_017352453.1 Danio rerio microtubule crosslinking factor 1 (mtcl1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa56507
    Clone ID Related Accession (Same CDS sequence) XM_005171457.2 , XM_005171457.3
    Accession Version XM_005171457.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5769bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product microtubule cross-linking factor 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005171457.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGAGAGCT CCAATGGCTC CGACACCAAA CCGCAGAGCC ATCATCCTCA GGTGGAGAAG 
    AAGAGGTTAA ACCGCGCGCC GTCCCCTGCC AGACCCGTCC TCAAGGATGT CCACTCGCGG
    ACGGCCAAAC CGATCGTGCC AAAATCGCCC AAATTCAACA AGCAGCAAGC GCCGGTGACG
    CCTCAGAGCC GCGTCCCGAG ACGCACCGTC CCGGGCGCGA AGGACAAAAC CGCATCAGCA
    CCTGCAAAAA GCAACACCAA GCCCGCCTCA GCGACAAAAA AGCCCGTTAA GGTCACTCAG
    CATGCAGCAT CCGAACCCAA ACCGGCCGGT AAGGCTGCGG AGTCCGCCGC TCCGCAGATC
    ACCAAGAGCA AGGGGAGACC TGCCCCCATA CGCGTGCCCA CCGGACGCGG CGTCTGTCAG
    ACCGACAGCA GCTCGGATTT GTCTGACTGT CCTTCCGAGC CGCTGTCCGA CGAGCAGAGG
    CTGGTCCAGG CCGCCAGCAG CGACGCGGAG TCGGGCAGCG GCTCGGGTTC GAGTGAGCAG
    GCGGCTGCAG GATCACGGAC TGCACCTGCT CCTGCTCCCG TTGCGCGCGC GGACAAACGC
    AAAGATAATA AAGACGCACA GTTTAAACCC GACGGCAAGG AGATGGAGAC GGAGATGGCC
    CAGGACGAGC TGCTAAGGGA GATCGAAGAC CTGCGATCGG AAAATGACTA CCTCAAGGAT
    GAAGTGGATG AGCTGCGAGC AGAGATGGAG GAGATCCGGG ACAGTTACCT GGAAGAGGAG
    GTGTACCAGC TGCAAGATCT GCGCCGGGAA CTGGACCGCT CCAACAAAAA CTGCCGGATC
    CTCCAGTACC GCCTGCGCAA GGCTGAACAG AAGAGCCTGC GTGTAGCACA GACCGGACAT
    GTGGACGGAG AGCTTCTGCG CGGCCTGGAG CAGGACCTCA AGGTGGCCAA AGATGTATCG
    GTGCGTCTGC ACAATGAACT AGAAACGGTG GAAGACAAGC GAACGCGGGC GGAAGATGAA
    AACGAGCTGC TCAGGCAAAA GATCATCGAA ATCGAGATCT CCAAACAGGC CCTGCATAAT
    GAGCTGGAGA GAGCCAAAGA GACTGCTCTG CGGAGGCGAG GCAGTAGAGA AGCCTTCAAA
    GATAAGAAGC CTTTATCTCA GGAGGACAGT GCAGATCTGC GCTGTCAGCT ACAGTTTGCC
    AAGGAGGAAT CGACTCTCAT GCGTAAAAAG ATGGCCAAAC TTGGCCGCGA GAAGGATGAA
    CTGGAACAAG AATTGCAGAA GTATAAATCA GTGTACGGCG ACGTGGACAG CCCTTTGCCT
    TTAGCGGAGG TGACCGGCGG AGGCCCACAC ACCACACGCG AAGCTGAGCT ACGACTACGA
    CTTAAACTAG TAGAAGAAGA AGCAAACATC CTGGGGAGGA AGATTGTGGA GCTGGAAGTT
    GAAAACCGCG GTCTCAGAGC GGAAAATGAA GACTTGCGTA GTCAATATGA AAGGGATTGC
    TTTGGCCGTG AACCCTTCTC CAGCGTCCCT ACCTCACCGT TTGGCGGCGA TGCGCTTGAG
    TCTGCGAGTG AACTGCGCAG ACATTTGCAG TTTGTGGAGG AAGAAGCTGA GCTCCTGCGG
    CGCTCCATTT CTGAAATCGA AGATCACAAC AAACAGCTAA CATCCGAGCT CAACCGCTTC
    AAATTCGGTC CCAATCAATC CGAGCGGGAA TCCGAAGGAG CTGAAGGAGG ACTCAAAATT
    GTGAATACCG CAGGTAATGG TTTTTCAAGC AGCACACTTC AGGATGAACT AAAAAACGCA
    AGACTCCAAG TCAACGAGTT AAGCGGGAAA GTAATGAAGC TTCAATATGA GAATCGGGTG
    CTAATCTCTA ATATGCAGCG CTGTGATTTG GCCGCACACT TGGGAATGCG TACTGGTAGT
    CCACGGGATA GTGATGTAGA CAGCGACGCT GGCCGAAGGG AACCAGATGA AGATGAAACA
    GCAAGGATAT TGCTGCTCCA TCCCAAACGT GAAGGTCCTG TTGGCGGAGA GAGCGATTCT
    GATGATTTAT TTGAACGTAC GGCTACTTCA GGATTCGCCA GTGGTGAAAA ACCTTCAGAT
    TCTGGCGAAC TGGGAGTAAT CGAATTAGCT CAGCGTAGAC GGGAAGACCG GGAAGCGTTT
    AATAATATCA GAAGGGAAGC TGAGAGGCTC GGGAAAACAG TAGAGCGGCT CATAACGGAT
    ACGGATAGTC TTATCCGGGA AGGTCGACTG GTTGTTTTGG GAGGAGATCT TCTTGCGGAG
    GGTGTGGAAT TTAGAGGCGA CGGAGACTCA GCTGAATCTA AACAAGACTC TCAGGTGCTG
    GACTCCATTA ATGCACGCAT GAGGACTTTC CGAACCGATC TCCAGCAATT CATGGATAAA
    GTGGAGCATC TAGGGGATGG TTTACGGGAT CGGGTTGATG ATCTTTCCCC GATGCCTAAC
    CTCACAGAAT CTAGCAGCTT CCTTTCTACG GTTACCTCCA TGTCCAGGGA TTCGCCAATC
    GGGACACTGG GAAGGGATCT AGTGACGGAC TTCCAGTCCG GGCGCAGGGA GGAGCAGGAG
    AGCTGTGTAG ACCGGCCCAG AGGAGCCGCG GGACTCTCTA GATACACCAG ACCACACACT
    TTCAGATCCG AGTTTCGCGA CCCTGTCCTG CCTGAGCTGG GAACGCCAAC CCGAGAGATA
    CAGCTCCGTC TGGACCACGA GCGGAGATTG AGAGCTGGAG CAGAGGACAG AGAAGGCTTT
    ACTTCATCTA TCACTCAGGA GGAGGAGAGG AGGCGTGCAG AGCTCCGAAG AGGACTCGAG
    CTGCAAGGGC TTCAGTCTCA TGAGCCAACA TGGCCACAGG AAAGAGGCCT GCTGCAACAG
    GAAGTGAGGT TGTTCCGCCA CAACATGGTG ATTGTGTACA TGAAGCTGCG CTGGATCCTC
    ATGCACTGGA GACTCGGACG CAGGACGGAC ACCATGGAGG AGGGAGCACA TGCTGAGTAT
    GAACGACTGG AGAATATTCC AGAACTGGGC ATTATGATCG ATCAGGGTGA CGGAGACACG
    GAGCGGGAAG ACAAAGTGCA TCCTCAAATA ACAGGAAGTG ACATTGCAGA GCCAGGACCA
    CTTCTGCTGC CATCGCCGGA GAGATTCCAG TACCAGAAAC AGGCGTGTGA CAGTCGGCGT
    GTTCTGCATG CGCTACGGTC TCTGCTGGAG GAGTTCAGGG CGGAGCTTCG GGAGGAGGCG
    GGGCGGCGCT GGCAGCTGCA GCAGGCGTTA GCCAATGAGA GAGCGGCATG GGAAATACAG
    CGCGCTGAAA TGAGGAGTCA CATCAACACG CTGCAGGATG CCAGAGGGGT TTCTACAGAT
    GCAGATGAAG GTGCAGTTTC TACAGATGTT TTACAGCTGG AGCGGGAAGA GCAGCGCCGC
    CTTCTGGCCG ACAGTCACAG CACGGCACTG GAACTGCGCT GGAAACTGCA GCACGGAGAA
    AAGCGCTGGA CTCGAGAGCG CAACGAACTT CTGGAGCGAT TTGAGAAAGA GCGGCAGGAA
    TGGGACCGCA GCTTACGGGA CATGCACCGC AAGATGGAAA AGATGCAGAA AGATGCTCTT
    CAGTCAGATG AGGGCGCAGC ACATGTCTTC TCCCCTCAGG AAAGCCCTTG TAAGGCTCCC
    AGATCTCCAC GTTCCCCCTG CATTGCAGCA CACACTCGCT CACACTCCGA CTCCGAGGCC
    ACACTGGAGG AGAAGGGAGC AGTAGGAAGA CCCAGGCCCT GTGAGGGACC CCGGCCCCCA
    GAGAGCCTGT TTCTGGATGC CCTCTCCCTG GACCCCCTGA GCGAAGCTGA GGTTCCCCCT
    CCATCACGTC TGGAGAGTGA AAAAAGGTTC CCCCACCTGA AGGAAGTAAG AGACCGAGCA
    CTGAATGAGA TTTCTGAGAT TGCGGATCCT GCCATTTACC CTGAAGAAGA GAAAAGTAGT
    GGCAGCTTAC TCAGGGCAAA GTCAGTCTGC TCCATGAGCG ACTTCCAACG TTTAATGGAC
    AGCTCTCCCT TTCTACCAGA CAAAAGTAAG ACTGGTGAGC ATGGGCGGGA TGATGTGACC
    CCTCCTCTTT CTCCAGACGA CCTCAAGTAC ATTGAGGAGT TTAATAGTAA GGGTTGGGAT
    CTGCCCACCA GCTCTCTAGC CACTTGCCCC ATTCCTGGCC CAGTAATCCC CTCTCCGGTG
    ATGGAGGCCT GGGCGGAGAG AGCAGAATCC CGAAGGGCAG ATTCTACTTC AGAAGCATTC
    CAGCAAGCCT CTTGGTTCTT GACCACCAGC GCTACCTTGA CCACCAACAC CATGAGTAGC
    CCTGAGTACT GCCAAAAGGT CCAACTCAGG GCAGCTCAGG GCAGTGACCA GTTTGGGGTG
    CATGTTCTTC ACAGTCCGTC TCGAGGGGAA CGATCCGCTC CGAGTCCTTC TGAGCAGGAA
    TACATGTTCT CCAAAGTGAC CAAAGTGAAG GCAGGGGAAG TAGTAGGGGG TGCAGAAGAA
    GTATTTGGAG GAAAATGGCC GTGTGAGGTT GGGAGTCATT TGGAGGCAGG ACTTAGGCCT
    GGTGAGCGTC CATCAATTTG TGCTGCAGTC GGCTATACCT CATCACTAGA GCTGGAGCTG
    TCACGCAATC TGAGCGATGA TATGAAGGAG AAGGCATATT CCGCCCGCAA TGCAATCCGA
    TCATCTACTA CCACTGGATC CAGCCCTCCG GAAAGGCAGC TACGGGACAT GGCATGCCAG
    ACGAATGGTC TGACCTCACG AGGAACACAG ACCACTCAGA CTATCAGTGT GGGACTGCAA
    ACTGAGGCTC TTCGCACCCT CACGAGCAGC CCGCATCGCT GTCTTACCCC CAAAGGAGGA
    GGCTCTACTC CCATCTCATC CCCTTCACGT AGCCTAAGAA AGATGCAGTA CTCTCCAGTC
    GTCCAAGCCA AGTTTGAACG GCCATGCTGT TCTCCAAAAT ACGGCTCACC CAAGCTTCAA
    AGGAAACCTT CTAATGCTAG TAAAACAGAA CAGCCTGTCA GCCAAACCCG TGCTCCCACC
    CCAACAACTC CTGTCCAGCA GCAAAAAGGC AATAACGAAT CCGCTTGGGC TCGTTCTACG
    ACCACCAGAG ATAGCCCCGT TCACACCACC ATCAACGACG GCCTCTCCAG CCTCTTCAAC
    ATCATCGACC ACACGCCCAT CGCCTACGAC CCCATGCAGA AGTTCAACAA ATCCCCAAGC
    CGCTCTCGTC CTACCGAATC AGGGCCAACC GAGCCCAAAT CTCCCAGTGT TAGTGCTGCA
    CAGGGGCCTT TGAGGAACTC TCGGGGCCGG TCCCCAAGCC CTGTGCAGCT GATTGTAGAG
    ACGCAGGGCG AGAAAACTCC AGAGGTGATT AGCATCAGAC AGGACCTGTC TGCTCCTCCG
    GGCTACACGT TAGCAGAGAA CGCAGCACGG ATATTGAATA AGAAACTACT GGAGCAGAGC
    TTCAGGGAGG AAAGGAGGCT TTCTGCTTCA TCCGCAGCTG TCCCGAACAG AACCGGAGAC
    TCAGAGAAAC CCGGATGTCT TGAGGATCTG CCACGTTCTC CTGTGGCTCC GCCTCTGGAC
    TCCTGCTTTC TGCGTCCGGC TCGTCCTGCC AATCGGCGTC CTCCGTCCCG CTGGGCGTCT
    CACTCCCCCT CATCCTCACC CAAATACAGC GAGCGCTCAG AGCGGTTCCT CTTCCCTTCG
    CCCGTCCGAC CCATTCACAC CATCCCAGAG ACTGAGGAGC CAAGCCTGGA GACCCGCCTG
    CAACCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005171514.2
    CDS510..6278
    Translation

    Target ORF information:

    RefSeq Version XM_005171457.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio microtubule crosslinking factor 1 (mtcl1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005171457.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGAGAGCT CCAATGGCTC CGACACCAAA CCGCAGAGCC ATCATCCTCA GGTGGAGAAG 
    AAGAGGTTAA ACCGCGCGCC GTCCCCTGCC AGACCCGTCC TCAAGGATGT CCACTCGCGG
    ACGGCCAAAC CGATCGTGCC AAAATCGCCC AAATTCAACA AGCAGCAAGC GCCGGTGACG
    CCTCAGAGCC GCGTCCCGAG ACGCACCGTC CCGGGCGCGA AGGACAAAAC CGCATCAGCA
    CCTGCAAAAA GCAACACCAA GCCCGCCTCA GCGACAAAAA AGCCCGTTAA GGTCACTCAG
    CATGCAGCAT CCGAACCCAA ACCGGCCGGT AAGGCTGCGG AGTCCGCCGC TCCGCAGATC
    ACCAAGAGCA AGGGGAGACC TGCCCCCATA CGCGTGCCCA CCGGACGCGG CGTCTGTCAG
    ACCGACAGCA GCTCGGATTT GTCTGACTGT CCTTCCGAGC CGCTGTCCGA CGAGCAGAGG
    CTGGTCCAGG CCGCCAGCAG CGACGCGGAG TCGGGCAGCG GCTCGGGTTC GAGTGAGCAG
    GCGGCTGCAG GATCACGGAC TGCACCTGCT CCTGCTCCCG TTGCGCGCGC GGACAAACGC
    AAAGATAATA AAGACGCACA GTTTAAACCC GACGGCAAGG AGATGGAGAC GGAGATGGCC
    CAGGACGAGC TGCTAAGGGA GATCGAAGAC CTGCGATCGG AAAATGACTA CCTCAAGGAT
    GAAGTGGATG AGCTGCGAGC AGAGATGGAG GAGATCCGGG ACAGTTACCT GGAAGAGGAG
    GTGTACCAGC TGCAAGATCT GCGCCGGGAA CTGGACCGCT CCAACAAAAA CTGCCGGATC
    CTCCAGTACC GCCTGCGCAA GGCTGAACAG AAGAGCCTGC GTGTAGCACA GACCGGACAT
    GTGGACGGAG AGCTTCTGCG CGGCCTGGAG CAGGACCTCA AGGTGGCCAA AGATGTATCG
    GTGCGTCTGC ACAATGAACT AGAAACGGTG GAAGACAAGC GAACGCGGGC GGAAGATGAA
    AACGAGCTGC TCAGGCAAAA GATCATCGAA ATCGAGATCT CCAAACAGGC CCTGCATAAT
    GAGCTGGAGA GAGCCAAAGA GACTGCTCTG CGGAGGCGAG GCAGTAGAGA AGCCTTCAAA
    GATAAGAAGC CTTTATCTCA GGAGGACAGT GCAGATCTGC GCTGTCAGCT ACAGTTTGCC
    AAGGAGGAAT CGACTCTCAT GCGTAAAAAG ATGGCCAAAC TTGGCCGCGA GAAGGATGAA
    CTGGAACAAG AATTGCAGAA GTATAAATCA GTGTACGGCG ACGTGGACAG CCCTTTGCCT
    TTAGCGGAGG TGACCGGCGG AGGCCCACAC ACCACACGCG AAGCTGAGCT ACGACTACGA
    CTTAAACTAG TAGAAGAAGA AGCAAACATC CTGGGGAGGA AGATTGTGGA GCTGGAAGTT
    GAAAACCGCG GTCTCAGAGC GGAAAATGAA GACTTGCGTA GTCAATATGA AAGGGATTGC
    TTTGGCCGTG AACCCTTCTC CAGCGTCCCT ACCTCACCGT TTGGCGGCGA TGCGCTTGAG
    TCTGCGAGTG AACTGCGCAG ACATTTGCAG TTTGTGGAGG AAGAAGCTGA GCTCCTGCGG
    CGCTCCATTT CTGAAATCGA AGATCACAAC AAACAGCTAA CATCCGAGCT CAACCGCTTC
    AAATTCGGTC CCAATCAATC CGAGCGGGAA TCCGAAGGAG CTGAAGGAGG ACTCAAAATT
    GTGAATACCG CAGGTAATGG TTTTTCAAGC AGCACACTTC AGGATGAACT AAAAAACGCA
    AGACTCCAAG TCAACGAGTT AAGCGGGAAA GTAATGAAGC TTCAATATGA GAATCGGGTG
    CTAATCTCTA ATATGCAGCG CTGTGATTTG GCCGCACACT TGGGAATGCG TACTGGTAGT
    CCACGGGATA GTGATGTAGA CAGCGACGCT GGCCGAAGGG AACCAGATGA AGATGAAACA
    GCAAGGATAT TGCTGCTCCA TCCCAAACGT GAAGGTCCTG TTGGCGGAGA GAGCGATTCT
    GATGATTTAT TTGAACGTAC GGCTACTTCA GGATTCGCCA GTGGTGAAAA ACCTTCAGAT
    TCTGGCGAAC TGGGAGTAAT CGAATTAGCT CAGCGTAGAC GGGAAGACCG GGAAGCGTTT
    AATAATATCA GAAGGGAAGC TGAGAGGCTC GGGAAAACAG TAGAGCGGCT CATAACGGAT
    ACGGATAGTC TTATCCGGGA AGGTCGACTG GTTGTTTTGG GAGGAGATCT TCTTGCGGAG
    GGTGTGGAAT TTAGAGGCGA CGGAGACTCA GCTGAATCTA AACAAGACTC TCAGGTGCTG
    GACTCCATTA ATGCACGCAT GAGGACTTTC CGAACCGATC TCCAGCAATT CATGGATAAA
    GTGGAGCATC TAGGGGATGG TTTACGGGAT CGGGTTGATG ATCTTTCCCC GATGCCTAAC
    CTCACAGAAT CTAGCAGCTT CCTTTCTACG GTTACCTCCA TGTCCAGGGA TTCGCCAATC
    GGGACACTGG GAAGGGATCT AGTGACGGAC TTCCAGTCCG GGCGCAGGGA GGAGCAGGAG
    AGCTGTGTAG ACCGGCCCAG AGGAGCCGCG GGACTCTCTA GATACACCAG ACCACACACT
    TTCAGATCCG AGTTTCGCGA CCCTGTCCTG CCTGAGCTGG GAACGCCAAC CCGAGAGATA
    CAGCTCCGTC TGGACCACGA GCGGAGATTG AGAGCTGGAG CAGAGGACAG AGAAGGCTTT
    ACTTCATCTA TCACTCAGGA GGAGGAGAGG AGGCGTGCAG AGCTCCGAAG AGGACTCGAG
    CTGCAAGGGC TTCAGTCTCA TGAGCCAACA TGGCCACAGG AAAGAGGCCT GCTGCAACAG
    GAAGTGAGGT TGTTCCGCCA CAACATGGTG ATTGTGTACA TGAAGCTGCG CTGGATCCTC
    ATGCACTGGA GACTCGGACG CAGGACGGAC ACCATGGAGG AGGGAGCACA TGCTGAGTAT
    GAACGACTGG AGAATATTCC AGAACTGGGC ATTATGATCG ATCAGGGTGA CGGAGACACG
    GAGCGGGAAG ACAAAGTGCA TCCTCAAATA ACAGGAAGTG ACATTGCAGA GCCAGGACCA
    CTTCTGCTGC CATCGCCGGA GAGATTCCAG TACCAGAAAC AGGCGTGTGA CAGTCGGCGT
    GTTCTGCATG CGCTACGGTC TCTGCTGGAG GAGTTCAGGG CGGAGCTTCG GGAGGAGGCG
    GGGCGGCGCT GGCAGCTGCA GCAGGCGTTA GCCAATGAGA GAGCGGCATG GGAAATACAG
    CGCGCTGAAA TGAGGAGTCA CATCAACACG CTGCAGGATG CCAGAGGGGT TTCTACAGAT
    GCAGATGAAG GTGCAGTTTC TACAGATGTT TTACAGCTGG AGCGGGAAGA GCAGCGCCGC
    CTTCTGGCCG ACAGTCACAG CACGGCACTG GAACTGCGCT GGAAACTGCA GCACGGAGAA
    AAGCGCTGGA CTCGAGAGCG CAACGAACTT CTGGAGCGAT TTGAGAAAGA GCGGCAGGAA
    TGGGACCGCA GCTTACGGGA CATGCACCGC AAGATGGAAA AGATGCAGAA AGATGCTCTT
    CAGTCAGATG AGGGCGCAGC ACATGTCTTC TCCCCTCAGG AAAGCCCTTG TAAGGCTCCC
    AGATCTCCAC GTTCCCCCTG CATTGCAGCA CACACTCGCT CACACTCCGA CTCCGAGGCC
    ACACTGGAGG AGAAGGGAGC AGTAGGAAGA CCCAGGCCCT GTGAGGGACC CCGGCCCCCA
    GAGAGCCTGT TTCTGGATGC CCTCTCCCTG GACCCCCTGA GCGAAGCTGA GGTTCCCCCT
    CCATCACGTC TGGAGAGTGA AAAAAGGTTC CCCCACCTGA AGGAAGTAAG AGACCGAGCA
    CTGAATGAGA TTTCTGAGAT TGCGGATCCT GCCATTTACC CTGAAGAAGA GAAAAGTAGT
    GGCAGCTTAC TCAGGGCAAA GTCAGTCTGC TCCATGAGCG ACTTCCAACG TTTAATGGAC
    AGCTCTCCCT TTCTACCAGA CAAAAGTAAG ACTGGTGAGC ATGGGCGGGA TGATGTGACC
    CCTCCTCTTT CTCCAGACGA CCTCAAGTAC ATTGAGGAGT TTAATAGTAA GGGTTGGGAT
    CTGCCCACCA GCTCTCTAGC CACTTGCCCC ATTCCTGGCC CAGTAATCCC CTCTCCGGTG
    ATGGAGGCCT GGGCGGAGAG AGCAGAATCC CGAAGGGCAG ATTCTACTTC AGAAGCATTC
    CAGCAAGCCT CTTGGTTCTT GACCACCAGC GCTACCTTGA CCACCAACAC CATGAGTAGC
    CCTGAGTACT GCCAAAAGGT CCAACTCAGG GCAGCTCAGG GCAGTGACCA GTTTGGGGTG
    CATGTTCTTC ACAGTCCGTC TCGAGGGGAA CGATCCGCTC CGAGTCCTTC TGAGCAGGAA
    TACATGTTCT CCAAAGTGAC CAAAGTGAAG GCAGGGGAAG TAGTAGGGGG TGCAGAAGAA
    GTATTTGGAG GAAAATGGCC GTGTGAGGTT GGGAGTCATT TGGAGGCAGG ACTTAGGCCT
    GGTGAGCGTC CATCAATTTG TGCTGCAGTC GGCTATACCT CATCACTAGA GCTGGAGCTG
    TCACGCAATC TGAGCGATGA TATGAAGGAG AAGGCATATT CCGCCCGCAA TGCAATCCGA
    TCATCTACTA CCACTGGATC CAGCCCTCCG GAAAGGCAGC TACGGGACAT GGCATGCCAG
    ACGAATGGTC TGACCTCACG AGGAACACAG ACCACTCAGA CTATCAGTGT GGGACTGCAA
    ACTGAGGCTC TTCGCACCCT CACGAGCAGC CCGCATCGCT GTCTTACCCC CAAAGGAGGA
    GGCTCTACTC CCATCTCATC CCCTTCACGT AGCCTAAGAA AGATGCAGTA CTCTCCAGTC
    GTCCAAGCCA AGTTTGAACG GCCATGCTGT TCTCCAAAAT ACGGCTCACC CAAGCTTCAA
    AGGAAACCTT CTAATGCTAG TAAAACAGAA CAGCCTGTCA GCCAAACCCG TGCTCCCACC
    CCAACAACTC CTGTCCAGCA GCAAAAAGGC AATAACGAAT CCGCTTGGGC TCGTTCTACG
    ACCACCAGAG ATAGCCCCGT TCACACCACC ATCAACGACG GCCTCTCCAG CCTCTTCAAC
    ATCATCGACC ACACGCCCAT CGCCTACGAC CCCATGCAGA AGTTCAACAA ATCCCCAAGC
    CGCTCTCGTC CTACCGAATC AGGGCCAACC GAGCCCAAAT CTCCCAGTGT TAGTGCTGCA
    CAGGGGCCTT TGAGGAACTC TCGGGGCCGG TCCCCAAGCC CTGTGCAGCT GATTGTAGAG
    ACGCAGGGCG AGAAAACTCC AGAGGTGATT AGCATCAGAC AGGACCTGTC TGCTCCTCCG
    GGCTACACGT TAGCAGAGAA CGCAGCACGG ATATTGAATA AGAAACTACT GGAGCAGAGC
    TTCAGGGAGG AAAGGAGGCT TTCTGCTTCA TCCGCAGCTG TCCCGAACAG AACCGGAGAC
    TCAGAGAAAC CCGGATGTCT TGAGGATCTG CCACGTTCTC CTGTGGCTCC GCCTCTGGAC
    TCCTGCTTTC TGCGTCCGGC TCGTCCTGCC AATCGGCGTC CTCCGTCCCG CTGGGCGTCT
    CACTCCCCCT CATCCTCACC CAAATACAGC GAGCGCTCAG AGCGGTTCCT CTTCCCTTCG
    CCCGTCCGAC CCATTCACAC CATCCCAGAG ACTGAGGAGC CAAGCCTGGA GACCCGCCTG
    CAACCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa56507
    Clone ID Related Accession (Same CDS sequence) XM_005171457.2 , XM_005171457.3
    Accession Version XM_005171457.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5769bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product microtubule cross-linking factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005171457.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGAGAGCT CCAATGGCTC CGACACCAAA CCGCAGAGCC ATCATCCTCA GGTGGAGAAG 
    AAGAGGTTAA ACCGCGCGCC GTCCCCTGCC AGACCCGTCC TCAAGGATGT CCACTCGCGG
    ACGGCCAAAC CGATCGTGCC AAAATCGCCC AAATTCAACA AGCAGCAAGC GCCGGTGACG
    CCTCAGAGCC GCGTCCCGAG ACGCACCGTC CCGGGCGCGA AGGACAAAAC CGCATCAGCA
    CCTGCAAAAA GCAACACCAA GCCCGCCTCA GCGACAAAAA AGCCCGTTAA GGTCACTCAG
    CATGCAGCAT CCGAACCCAA ACCGGCCGGT AAGGCTGCGG AGTCCGCCGC TCCGCAGATC
    ACCAAGAGCA AGGGGAGACC TGCCCCCATA CGCGTGCCCA CCGGACGCGG CGTCTGTCAG
    ACCGACAGCA GCTCGGATTT GTCTGACTGT CCTTCCGAGC CGCTGTCCGA CGAGCAGAGG
    CTGGTCCAGG CCGCCAGCAG CGACGCGGAG TCGGGCAGCG GCTCGGGTTC GAGTGAGCAG
    GCGGCTGCAG GATCACGGAC TGCACCTGCT CCTGCTCCCG TTGCGCGCGC GGACAAACGC
    AAAGATAATA AAGACGCACA GTTTAAACCC GACGGCAAGG AGATGGAGAC GGAGATGGCC
    CAGGACGAGC TGCTAAGGGA GATCGAAGAC CTGCGATCGG AAAATGACTA CCTCAAGGAT
    GAAGTGGATG AGCTGCGAGC AGAGATGGAG GAGATCCGGG ACAGTTACCT GGAAGAGGAG
    GTGTACCAGC TGCAAGATCT GCGCCGGGAA CTGGACCGCT CCAACAAAAA CTGCCGGATC
    CTCCAGTACC GCCTGCGCAA GGCTGAACAG AAGAGCCTGC GTGTAGCACA GACCGGACAT
    GTGGACGGAG AGCTTCTGCG CGGCCTGGAG CAGGACCTCA AGGTGGCCAA AGATGTATCG
    GTGCGTCTGC ACAATGAACT AGAAACGGTG GAAGACAAGC GAACGCGGGC GGAAGATGAA
    AACGAGCTGC TCAGGCAAAA GATCATCGAA ATCGAGATCT CCAAACAGGC CCTGCATAAT
    GAGCTGGAGA GAGCCAAAGA GACTGCTCTG CGGAGGCGAG GCAGTAGAGA AGCCTTCAAA
    GATAAGAAGC CTTTATCTCA GGAGGACAGT GCAGATCTGC GCTGTCAGCT ACAGTTTGCC
    AAGGAGGAAT CGACTCTCAT GCGTAAAAAG ATGGCCAAAC TTGGCCGCGA GAAGGATGAA
    CTGGAACAAG AATTGCAGAA GTATAAATCA GTGTACGGCG ACGTGGACAG CCCTTTGCCT
    TTAGCGGAGG TGACCGGCGG AGGCCCACAC ACCACACGCG AAGCTGAGCT ACGACTACGA
    CTTAAACTAG TAGAAGAAGA AGCAAACATC CTGGGGAGGA AGATTGTGGA GCTGGAAGTT
    GAAAACCGCG GTCTCAGAGC GGAAAATGAA GACTTGCGTA GTCAATATGA AAGGGATTGC
    TTTGGCCGTG AACCCTTCTC CAGCGTCCCT ACCTCACCGT TTGGCGGCGA TGCGCTTGAG
    TCTGCGAGTG AACTGCGCAG ACATTTGCAG TTTGTGGAGG AAGAAGCTGA GCTCCTGCGG
    CGCTCCATTT CTGAAATCGA AGATCACAAC AAACAGCTAA CATCCGAGCT CAACCGCTTC
    AAATTCGGTC CCAATCAATC CGAGCGGGAA TCCGAAGGAG CTGAAGGAGG ACTCAAAATT
    GTGAATACCG CAGGTAATGG TTTTTCAAGC AGCACACTTC AGGATGAACT AAAAAACGCA
    AGACTCCAAG TCAACGAGTT AAGCGGGAAA GTAATGAAGC TTCAATATGA GAATCGGGTG
    CTAATCTCTA ATATGCAGCG CTGTGATTTG GCCGCACACT TGGGAATGCG TACTGGTAGT
    CCACGGGATA GTGATGTAGA CAGCGACGCT GGCCGAAGGG AACCAGATGA AGATGAAACA
    GCAAGGATAT TGCTGCTCCA TCCCAAACGT GAAGGTCCTG TTGGCGGAGA GAGCGATTCT
    GATGATTTAT TTGAACGTAC GGCTACTTCA GGATTCGCCA GTGGTGAAAA ACCTTCAGAT
    TCTGGCGAAC TGGGAGTAAT CGAATTAGCT CAGCGTAGAC GGGAAGACCG GGAAGCGTTT
    AATAATATCA GAAGGGAAGC TGAGAGGCTC GGGAAAACAG TAGAGCGGCT CATAACGGAT
    ACGGATAGTC TTATCCGGGA AGGTCGACTG GTTGTTTTGG GAGGAGATCT TCTTGCGGAG
    GGTGTGGAAT TTAGAGGCGA CGGAGACTCA GCTGAATCTA AACAAGACTC TCAGGTGCTG
    GACTCCATTA ATGCACGCAT GAGGACTTTC CGAACCGATC TCCAGCAATT CATGGATAAA
    GTGGAGCATC TAGGGGATGG TTTACGGGAT CGGGTTGATG ATCTTTCCCC GATGCCTAAC
    CTCACAGAAT CTAGCAGCTT CCTTTCTACG GTTACCTCCA TGTCCAGGGA TTCGCCAATC
    GGGACACTGG GAAGGGATCT AGTGACGGAC TTCCAGTCCG GGCGCAGGGA GGAGCAGGAG
    AGCTGTGTAG ACCGGCCCAG AGGAGCCGCG GGACTCTCTA GATACACCAG ACCACACACT
    TTCAGATCCG AGTTTCGCGA CCCTGTCCTG CCTGAGCTGG GAACGCCAAC CCGAGAGATA
    CAGCTCCGTC TGGACCACGA GCGGAGATTG AGAGCTGGAG CAGAGGACAG AGAAGGCTTT
    ACTTCATCTA TCACTCAGGA GGAGGAGAGG AGGCGTGCAG AGCTCCGAAG AGGACTCGAG
    CTGCAAGGGC TTCAGTCTCA TGAGCCAACA TGGCCACAGG AAAGAGGCCT GCTGCAACAG
    GAAGTGAGGT TGTTCCGCCA CAACATGGTG ATTGTGTACA TGAAGCTGCG CTGGATCCTC
    ATGCACTGGA GACTCGGACG CAGGACGGAC ACCATGGAGG AGGGAGCACA TGCTGAGTAT
    GAACGACTGG AGAATATTCC AGAACTGGGC ATTATGATCG ATCAGGGTGA CGGAGACACG
    GAGCGGGAAG ACAAAGTGCA TCCTCAAATA ACAGGAAGTG ACATTGCAGA GCCAGGACCA
    CTTCTGCTGC CATCGCCGGA GAGATTCCAG TACCAGAAAC AGGCGTGTGA CAGTCGGCGT
    GTTCTGCATG CGCTACGGTC TCTGCTGGAG GAGTTCAGGG CGGAGCTTCG GGAGGAGGCG
    GGGCGGCGCT GGCAGCTGCA GCAGGCGTTA GCCAATGAGA GAGCGGCATG GGAAATACAG
    CGCGCTGAAA TGAGGAGTCA CATCAACACG CTGCAGGATG CCAGAGGGGT TTCTACAGAT
    GCAGATGAAG GTGCAGTTTC TACAGATGTT TTACAGCTGG AGCGGGAAGA GCAGCGCCGC
    CTTCTGGCCG ACAGTCACAG CACGGCACTG GAACTGCGCT GGAAACTGCA GCACGGAGAA
    AAGCGCTGGA CTCGAGAGCG CAACGAACTT CTGGAGCGAT TTGAGAAAGA GCGGCAGGAA
    TGGGACCGCA GCTTACGGGA CATGCACCGC AAGATGGAAA AGATGCAGAA AGATGCTCTT
    CAGTCAGATG AGGGCGCAGC ACATGTCTTC TCCCCTCAGG AAAGCCCTTG TAAGGCTCCC
    AGATCTCCAC GTTCCCCCTG CATTGCAGCA CACACTCGCT CACACTCCGA CTCCGAGGCC
    ACACTGGAGG AGAAGGGAGC AGTAGGAAGA CCCAGGCCCT GTGAGGGACC CCGGCCCCCA
    GAGAGCCTGT TTCTGGATGC CCTCTCCCTG GACCCCCTGA GCGAAGCTGA GGTTCCCCCT
    CCATCACGTC TGGAGAGTGA AAAAAGGTTC CCCCACCTGA AGGAAGTAAG AGACCGAGCA
    CTGAATGAGA TTTCTGAGAT TGCGGATCCT GCCATTTACC CTGAAGAAGA GAAAAGTAGT
    GGCAGCTTAC TCAGGGCAAA GTCAGTCTGC TCCATGAGCG ACTTCCAACG TTTAATGGAC
    AGCTCTCCCT TTCTACCAGA CAAAAGTAAG ACTGGTGAGC ATGGGCGGGA TGATGTGACC
    CCTCCTCTTT CTCCAGACGA CCTCAAGTAC ATTGAGGAGT TTAATAGTAA GGGTTGGGAT
    CTGCCCACCA GCTCTCTAGC CACTTGCCCC ATTCCTGGCC CAGTAATCCC CTCTCCGGTG
    ATGGAGGCCT GGGCGGAGAG AGCAGAATCC CGAAGGGCAG ATTCTACTTC AGAAGCATTC
    CAGCAAGCCT CTTGGTTCTT GACCACCAGC GCTACCTTGA CCACCAACAC CATGAGTAGC
    CCTGAGTACT GCCAAAAGGT CCAACTCAGG GCAGCTCAGG GCAGTGACCA GTTTGGGGTG
    CATGTTCTTC ACAGTCCGTC TCGAGGGGAA CGATCCGCTC CGAGTCCTTC TGAGCAGGAA
    TACATGTTCT CCAAAGTGAC CAAAGTGAAG GCAGGGGAAG TAGTAGGGGG TGCAGAAGAA
    GTATTTGGAG GAAAATGGCC GTGTGAGGTT GGGAGTCATT TGGAGGCAGG ACTTAGGCCT
    GGTGAGCGTC CATCAATTTG TGCTGCAGTC GGCTATACCT CATCACTAGA GCTGGAGCTG
    TCACGCAATC TGAGCGATGA TATGAAGGAG AAGGCATATT CCGCCCGCAA TGCAATCCGA
    TCATCTACTA CCACTGGATC CAGCCCTCCG GAAAGGCAGC TACGGGACAT GGCATGCCAG
    ACGAATGGTC TGACCTCACG AGGAACACAG ACCACTCAGA CTATCAGTGT GGGACTGCAA
    ACTGAGGCTC TTCGCACCCT CACGAGCAGC CCGCATCGCT GTCTTACCCC CAAAGGAGGA
    GGCTCTACTC CCATCTCATC CCCTTCACGT AGCCTAAGAA AGATGCAGTA CTCTCCAGTC
    GTCCAAGCCA AGTTTGAACG GCCATGCTGT TCTCCAAAAT ACGGCTCACC CAAGCTTCAA
    AGGAAACCTT CTAATGCTAG TAAAACAGAA CAGCCTGTCA GCCAAACCCG TGCTCCCACC
    CCAACAACTC CTGTCCAGCA GCAAAAAGGC AATAACGAAT CCGCTTGGGC TCGTTCTACG
    ACCACCAGAG ATAGCCCCGT TCACACCACC ATCAACGACG GCCTCTCCAG CCTCTTCAAC
    ATCATCGACC ACACGCCCAT CGCCTACGAC CCCATGCAGA AGTTCAACAA ATCCCCAAGC
    CGCTCTCGTC CTACCGAATC AGGGCCAACC GAGCCCAAAT CTCCCAGTGT TAGTGCTGCA
    CAGGGGCCTT TGAGGAACTC TCGGGGCCGG TCCCCAAGCC CTGTGCAGCT GATTGTAGAG
    ACGCAGGGCG AGAAAACTCC AGAGGTGATT AGCATCAGAC AGGACCTGTC TGCTCCTCCG
    GGCTACACGT TAGCAGAGAA CGCAGCACGG ATATTGAATA AGAAACTACT GGAGCAGAGC
    TTCAGGGAGG AAAGGAGGCT TTCTGCTTCA TCCGCAGCTG TCCCGAACAG AACCGGAGAC
    TCAGAGAAAC CCGGATGTCT TGAGGATCTG CCACGTTCTC CTGTGGCTCC GCCTCTGGAC
    TCCTGCTTTC TGCGTCCGGC TCGTCCTGCC AATCGGCGTC CTCCGTCCCG CTGGGCGTCT
    CACTCCCCCT CATCCTCACC CAAATACAGC GAGCGCTCAG AGCGGTTCCT CTTCCCTTCG
    CCCGTCCGAC CCATTCACAC CATCCCAGAG ACTGAGGAGC CAAGCCTGGA GACCCGCCTG
    CAACCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005171514.2
    CDS480..6248
    Translation

    Target ORF information:

    RefSeq Version XM_005171457.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio microtubule crosslinking factor 1 (mtcl1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005171457.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGAGAGCT CCAATGGCTC CGACACCAAA CCGCAGAGCC ATCATCCTCA GGTGGAGAAG 
    AAGAGGTTAA ACCGCGCGCC GTCCCCTGCC AGACCCGTCC TCAAGGATGT CCACTCGCGG
    ACGGCCAAAC CGATCGTGCC AAAATCGCCC AAATTCAACA AGCAGCAAGC GCCGGTGACG
    CCTCAGAGCC GCGTCCCGAG ACGCACCGTC CCGGGCGCGA AGGACAAAAC CGCATCAGCA
    CCTGCAAAAA GCAACACCAA GCCCGCCTCA GCGACAAAAA AGCCCGTTAA GGTCACTCAG
    CATGCAGCAT CCGAACCCAA ACCGGCCGGT AAGGCTGCGG AGTCCGCCGC TCCGCAGATC
    ACCAAGAGCA AGGGGAGACC TGCCCCCATA CGCGTGCCCA CCGGACGCGG CGTCTGTCAG
    ACCGACAGCA GCTCGGATTT GTCTGACTGT CCTTCCGAGC CGCTGTCCGA CGAGCAGAGG
    CTGGTCCAGG CCGCCAGCAG CGACGCGGAG TCGGGCAGCG GCTCGGGTTC GAGTGAGCAG
    GCGGCTGCAG GATCACGGAC TGCACCTGCT CCTGCTCCCG TTGCGCGCGC GGACAAACGC
    AAAGATAATA AAGACGCACA GTTTAAACCC GACGGCAAGG AGATGGAGAC GGAGATGGCC
    CAGGACGAGC TGCTAAGGGA GATCGAAGAC CTGCGATCGG AAAATGACTA CCTCAAGGAT
    GAAGTGGATG AGCTGCGAGC AGAGATGGAG GAGATCCGGG ACAGTTACCT GGAAGAGGAG
    GTGTACCAGC TGCAAGATCT GCGCCGGGAA CTGGACCGCT CCAACAAAAA CTGCCGGATC
    CTCCAGTACC GCCTGCGCAA GGCTGAACAG AAGAGCCTGC GTGTAGCACA GACCGGACAT
    GTGGACGGAG AGCTTCTGCG CGGCCTGGAG CAGGACCTCA AGGTGGCCAA AGATGTATCG
    GTGCGTCTGC ACAATGAACT AGAAACGGTG GAAGACAAGC GAACGCGGGC GGAAGATGAA
    AACGAGCTGC TCAGGCAAAA GATCATCGAA ATCGAGATCT CCAAACAGGC CCTGCATAAT
    GAGCTGGAGA GAGCCAAAGA GACTGCTCTG CGGAGGCGAG GCAGTAGAGA AGCCTTCAAA
    GATAAGAAGC CTTTATCTCA GGAGGACAGT GCAGATCTGC GCTGTCAGCT ACAGTTTGCC
    AAGGAGGAAT CGACTCTCAT GCGTAAAAAG ATGGCCAAAC TTGGCCGCGA GAAGGATGAA
    CTGGAACAAG AATTGCAGAA GTATAAATCA GTGTACGGCG ACGTGGACAG CCCTTTGCCT
    TTAGCGGAGG TGACCGGCGG AGGCCCACAC ACCACACGCG AAGCTGAGCT ACGACTACGA
    CTTAAACTAG TAGAAGAAGA AGCAAACATC CTGGGGAGGA AGATTGTGGA GCTGGAAGTT
    GAAAACCGCG GTCTCAGAGC GGAAAATGAA GACTTGCGTA GTCAATATGA AAGGGATTGC
    TTTGGCCGTG AACCCTTCTC CAGCGTCCCT ACCTCACCGT TTGGCGGCGA TGCGCTTGAG
    TCTGCGAGTG AACTGCGCAG ACATTTGCAG TTTGTGGAGG AAGAAGCTGA GCTCCTGCGG
    CGCTCCATTT CTGAAATCGA AGATCACAAC AAACAGCTAA CATCCGAGCT CAACCGCTTC
    AAATTCGGTC CCAATCAATC CGAGCGGGAA TCCGAAGGAG CTGAAGGAGG ACTCAAAATT
    GTGAATACCG CAGGTAATGG TTTTTCAAGC AGCACACTTC AGGATGAACT AAAAAACGCA
    AGACTCCAAG TCAACGAGTT AAGCGGGAAA GTAATGAAGC TTCAATATGA GAATCGGGTG
    CTAATCTCTA ATATGCAGCG CTGTGATTTG GCCGCACACT TGGGAATGCG TACTGGTAGT
    CCACGGGATA GTGATGTAGA CAGCGACGCT GGCCGAAGGG AACCAGATGA AGATGAAACA
    GCAAGGATAT TGCTGCTCCA TCCCAAACGT GAAGGTCCTG TTGGCGGAGA GAGCGATTCT
    GATGATTTAT TTGAACGTAC GGCTACTTCA GGATTCGCCA GTGGTGAAAA ACCTTCAGAT
    TCTGGCGAAC TGGGAGTAAT CGAATTAGCT CAGCGTAGAC GGGAAGACCG GGAAGCGTTT
    AATAATATCA GAAGGGAAGC TGAGAGGCTC GGGAAAACAG TAGAGCGGCT CATAACGGAT
    ACGGATAGTC TTATCCGGGA AGGTCGACTG GTTGTTTTGG GAGGAGATCT TCTTGCGGAG
    GGTGTGGAAT TTAGAGGCGA CGGAGACTCA GCTGAATCTA AACAAGACTC TCAGGTGCTG
    GACTCCATTA ATGCACGCAT GAGGACTTTC CGAACCGATC TCCAGCAATT CATGGATAAA
    GTGGAGCATC TAGGGGATGG TTTACGGGAT CGGGTTGATG ATCTTTCCCC GATGCCTAAC
    CTCACAGAAT CTAGCAGCTT CCTTTCTACG GTTACCTCCA TGTCCAGGGA TTCGCCAATC
    GGGACACTGG GAAGGGATCT AGTGACGGAC TTCCAGTCCG GGCGCAGGGA GGAGCAGGAG
    AGCTGTGTAG ACCGGCCCAG AGGAGCCGCG GGACTCTCTA GATACACCAG ACCACACACT
    TTCAGATCCG AGTTTCGCGA CCCTGTCCTG CCTGAGCTGG GAACGCCAAC CCGAGAGATA
    CAGCTCCGTC TGGACCACGA GCGGAGATTG AGAGCTGGAG CAGAGGACAG AGAAGGCTTT
    ACTTCATCTA TCACTCAGGA GGAGGAGAGG AGGCGTGCAG AGCTCCGAAG AGGACTCGAG
    CTGCAAGGGC TTCAGTCTCA TGAGCCAACA TGGCCACAGG AAAGAGGCCT GCTGCAACAG
    GAAGTGAGGT TGTTCCGCCA CAACATGGTG ATTGTGTACA TGAAGCTGCG CTGGATCCTC
    ATGCACTGGA GACTCGGACG CAGGACGGAC ACCATGGAGG AGGGAGCACA TGCTGAGTAT
    GAACGACTGG AGAATATTCC AGAACTGGGC ATTATGATCG ATCAGGGTGA CGGAGACACG
    GAGCGGGAAG ACAAAGTGCA TCCTCAAATA ACAGGAAGTG ACATTGCAGA GCCAGGACCA
    CTTCTGCTGC CATCGCCGGA GAGATTCCAG TACCAGAAAC AGGCGTGTGA CAGTCGGCGT
    GTTCTGCATG CGCTACGGTC TCTGCTGGAG GAGTTCAGGG CGGAGCTTCG GGAGGAGGCG
    GGGCGGCGCT GGCAGCTGCA GCAGGCGTTA GCCAATGAGA GAGCGGCATG GGAAATACAG
    CGCGCTGAAA TGAGGAGTCA CATCAACACG CTGCAGGATG CCAGAGGGGT TTCTACAGAT
    GCAGATGAAG GTGCAGTTTC TACAGATGTT TTACAGCTGG AGCGGGAAGA GCAGCGCCGC
    CTTCTGGCCG ACAGTCACAG CACGGCACTG GAACTGCGCT GGAAACTGCA GCACGGAGAA
    AAGCGCTGGA CTCGAGAGCG CAACGAACTT CTGGAGCGAT TTGAGAAAGA GCGGCAGGAA
    TGGGACCGCA GCTTACGGGA CATGCACCGC AAGATGGAAA AGATGCAGAA AGATGCTCTT
    CAGTCAGATG AGGGCGCAGC ACATGTCTTC TCCCCTCAGG AAAGCCCTTG TAAGGCTCCC
    AGATCTCCAC GTTCCCCCTG CATTGCAGCA CACACTCGCT CACACTCCGA CTCCGAGGCC
    ACACTGGAGG AGAAGGGAGC AGTAGGAAGA CCCAGGCCCT GTGAGGGACC CCGGCCCCCA
    GAGAGCCTGT TTCTGGATGC CCTCTCCCTG GACCCCCTGA GCGAAGCTGA GGTTCCCCCT
    CCATCACGTC TGGAGAGTGA AAAAAGGTTC CCCCACCTGA AGGAAGTAAG AGACCGAGCA
    CTGAATGAGA TTTCTGAGAT TGCGGATCCT GCCATTTACC CTGAAGAAGA GAAAAGTAGT
    GGCAGCTTAC TCAGGGCAAA GTCAGTCTGC TCCATGAGCG ACTTCCAACG TTTAATGGAC
    AGCTCTCCCT TTCTACCAGA CAAAAGTAAG ACTGGTGAGC ATGGGCGGGA TGATGTGACC
    CCTCCTCTTT CTCCAGACGA CCTCAAGTAC ATTGAGGAGT TTAATAGTAA GGGTTGGGAT
    CTGCCCACCA GCTCTCTAGC CACTTGCCCC ATTCCTGGCC CAGTAATCCC CTCTCCGGTG
    ATGGAGGCCT GGGCGGAGAG AGCAGAATCC CGAAGGGCAG ATTCTACTTC AGAAGCATTC
    CAGCAAGCCT CTTGGTTCTT GACCACCAGC GCTACCTTGA CCACCAACAC CATGAGTAGC
    CCTGAGTACT GCCAAAAGGT CCAACTCAGG GCAGCTCAGG GCAGTGACCA GTTTGGGGTG
    CATGTTCTTC ACAGTCCGTC TCGAGGGGAA CGATCCGCTC CGAGTCCTTC TGAGCAGGAA
    TACATGTTCT CCAAAGTGAC CAAAGTGAAG GCAGGGGAAG TAGTAGGGGG TGCAGAAGAA
    GTATTTGGAG GAAAATGGCC GTGTGAGGTT GGGAGTCATT TGGAGGCAGG ACTTAGGCCT
    GGTGAGCGTC CATCAATTTG TGCTGCAGTC GGCTATACCT CATCACTAGA GCTGGAGCTG
    TCACGCAATC TGAGCGATGA TATGAAGGAG AAGGCATATT CCGCCCGCAA TGCAATCCGA
    TCATCTACTA CCACTGGATC CAGCCCTCCG GAAAGGCAGC TACGGGACAT GGCATGCCAG
    ACGAATGGTC TGACCTCACG AGGAACACAG ACCACTCAGA CTATCAGTGT GGGACTGCAA
    ACTGAGGCTC TTCGCACCCT CACGAGCAGC CCGCATCGCT GTCTTACCCC CAAAGGAGGA
    GGCTCTACTC CCATCTCATC CCCTTCACGT AGCCTAAGAA AGATGCAGTA CTCTCCAGTC
    GTCCAAGCCA AGTTTGAACG GCCATGCTGT TCTCCAAAAT ACGGCTCACC CAAGCTTCAA
    AGGAAACCTT CTAATGCTAG TAAAACAGAA CAGCCTGTCA GCCAAACCCG TGCTCCCACC
    CCAACAACTC CTGTCCAGCA GCAAAAAGGC AATAACGAAT CCGCTTGGGC TCGTTCTACG
    ACCACCAGAG ATAGCCCCGT TCACACCACC ATCAACGACG GCCTCTCCAG CCTCTTCAAC
    ATCATCGACC ACACGCCCAT CGCCTACGAC CCCATGCAGA AGTTCAACAA ATCCCCAAGC
    CGCTCTCGTC CTACCGAATC AGGGCCAACC GAGCCCAAAT CTCCCAGTGT TAGTGCTGCA
    CAGGGGCCTT TGAGGAACTC TCGGGGCCGG TCCCCAAGCC CTGTGCAGCT GATTGTAGAG
    ACGCAGGGCG AGAAAACTCC AGAGGTGATT AGCATCAGAC AGGACCTGTC TGCTCCTCCG
    GGCTACACGT TAGCAGAGAA CGCAGCACGG ATATTGAATA AGAAACTACT GGAGCAGAGC
    TTCAGGGAGG AAAGGAGGCT TTCTGCTTCA TCCGCAGCTG TCCCGAACAG AACCGGAGAC
    TCAGAGAAAC CCGGATGTCT TGAGGATCTG CCACGTTCTC CTGTGGCTCC GCCTCTGGAC
    TCCTGCTTTC TGCGTCCGGC TCGTCCTGCC AATCGGCGTC CTCCGTCCCG CTGGGCGTCT
    CACTCCCCCT CATCCTCACC CAAATACAGC GAGCGCTCAG AGCGGTTCCT CTTCCCTTCG
    CCCGTCCGAC CCATTCACAC CATCCCAGAG ACTGAGGAGC CAAGCCTGGA GACCCGCCTG
    CAACCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa56508
    Clone ID Related Accession (Same CDS sequence) XM_017352451.2 , XM_017352451.1
    Accession Version XM_017352451.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5757bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product microtubule cross-linking factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017352451.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGAGAGCT CCAATGGCTC CGACACCAAA CCGCAGAGCC ATCATCCTCA GGTGGAGAAG 
    AAGAGGTTAA ACCGCGCGCC GTCCCCTGCC AGACCCGTCC TCAAGGATGT CCACTCGCGG
    ACGGCCAAAC CGATCGTGCC AAAATCGCCC AAATTCAACA AGCAGCAAGC GCCGGTGACG
    CCTCAGAGCC GCGTCCCGAG ACGCACCGTC CCGGGCGCGA AGGACAAAAC CGCATCAGCA
    CCTGCAAAAA GCAACACCAA GCCCGCCTCA GCGACAAAAA AGCCCGTTAA GGTCACTCAG
    CATGCAGCAT CCGAACCCAA ACCGGCCGGT AAGGCTGCGG AGTCCGCCGC TCCGCAGATC
    ACCAAGAGCA AGGGGAGACC TGCCCCCATA CGCGTGCCCA CCGGACGCGG CGTCTGTCAG
    ACCGACAGCA GCTCGGATTT GTCTGACTGT CCTTCCGAGC CGCTGTCCGA CGAGCAGAGG
    CTGGTCCAGG CCGCCAGCAG CGACGCGGAG TCGGGCAGCG GCTCGGGTTC GAGTGAGCAG
    GCGGCTGCAG GATCACGGAC TGCACCTGCT CCTGCTCCCG TTGCGCGCGC GGACAAACGC
    AAAGATAATA AAGACGCACA GTTTAAACCC GACGGCAAGG AGATGGAGAC GGAGATGGCC
    CAGGACGAGC TGCTAAGGGA GATCGAAGAC CTGCGATCGG AAAATGACTA CCTCAAGGAT
    GAAGTGGATG AGCTGCGAGC AGAGATGGAG GAGATCCGGG ACAGTTACCT GGAAGAGGAG
    GTGTACCAGC TGCAAGATCT GCGCCGGGAA CTGGACCGCT CCAACAAAAA CTGCCGGATC
    CTCCAGTACC GCCTGCGCAA GGCTGAACAG AAGAGCCTGC GTGTAGCACA GACCGGACAT
    GTGGACGGAG AGCTTCTGCG CGGCCTGGAG CAGGACCTCA AGGTGGCCAA AGATGTATCG
    GTGCGTCTGC ACAATGAACT AGAAACGGTG GAAGACAAGC GAACGCGGGC GGAAGATGAA
    AACGAGCTGC TCAGGCAAAA GATCATCGAA ATCGAGATCT CCAAACAGGC CCTGCATAAT
    GAGCTGGAGA GAGCCAAAGA GACTGCTCTG CGGAGGCGAG GCAGTAGAGA AGCCTTCAAA
    GATAAGAAGC CTTTATCTCA GGAGGACAGT GCAGATCTGC GCTGTCAGCT ACAGTTTGCC
    AAGGAGGAAT CGACTCTCAT GCGTAAAAAG ATGGCCAAAC TTGGCCGCGA GAAGGATGAA
    CTGGAACAAG AATTGCAGAA GTATAAATCA GTGTACGGCG ACGTGGACAG CCCTTTGCCT
    TTAGCGGAGG TGACCGGCGG AGGCCCACAC ACCACACGCG AAGCTGAGCT ACGACTACGA
    CTTAAACTAG TAGAAGAAGA AGCAAACATC CTGGGGAGGA AGATTGTGGA GCTGGAAGTT
    GAAAACCGCG GTCTCAGAGC GGAAAATGAA GACTTGCGTA GTCAATATGA AAGGGATTGC
    TTTGGCCGTG AACCCTTCTC CAGCGTCCCT ACCTCACCGT TTGGCGGCGA TGCGCTTGAG
    TCTGCGAGTG AACTGCGCAG ACATTTGCAG TTTGTGGAGG AAGAAGCTGA GCTCCTGCGG
    CGCTCCATTT CTGAAATCGA AGATCACAAC AAACAGCTAA CATCCGAGCT CAACCGCTTC
    AAATTCGGTC CCAATCAATC CGAGCGGGAA TCCGAAGGAG CTGAAGGAGG ACTCAAAATT
    GTGAATACCG CAGGTAATGG TTTTTCAAGC AGCACACTTC AGGATGAACT AAAAAACGCA
    AGACTCCAAG TCAACGAGTT AAGCGGGAAA GTAATGAAGC TTCAATATGA GAATCGGGTG
    CTAATCTCTA ATATGCAGCG CTGTGATTTG GCCGCACACT TGGGAATGCG TACTGGTAGT
    CCACGGGATA GTGATGTAGA CAGCGACGCT GGCCGAAGGG AACCAGATGA AGATGAAACA
    GCAAGGATAT TGCTGCTCCA TCCCAAACGT GAAGGTCCTG TTGGCGGAGA GAGCGATTCT
    GATGATTTAT TTGAACGTAC GGCTACTTCA GGATTCGCCA GTGGTGAAAA ACCTTCAGAT
    TCTGGCGAAC TGGGAGTAAT CGAATTAGCT CAGCGTAGAC GGGAAGACCG GGAAGCGTTT
    AATAATATCA GAAGGGAAGC TGAGAGGCTC GGGAAAACAG TAGAGCGGCT CATAACGGAT
    ACGGATAGTC TTATCCGGGA AGGTCGACTG GTTGTTTTGG GAGGAGATCT TCTTGCGGAG
    GGTGTGGAAT TTAGAGGCGA CGGAGACTCA GCTGAATCTA AACAAGACTC TCAGGTGCTG
    GACTCCATTA ATGCACGCAT GAGGACTTTC CGAACCGATC TCCAGCAATT CATGGATAAA
    GTGGAGCATC TAGGGGATGG TTTACGGGAT CGGGTTGATG ATCTTTCCCC GATGCCTAAC
    CTCACAGAAT CTAGCAGCTT CCTTTCTACG GTTACCTCCA TGTCCAGGGA TTCGCCAATC
    GGGACACTGG GAAGGGATCT AGTGACGGAC TTCCAGTCCG GGCGCAGGGA GGAGCAGGAG
    AGCTGTGTAG ACCGGCCCAG AGGAGCCGCG GGACTCTCTA GATACACCAG ACCACACACT
    TTCAGATCCG AGTTTCGCGA CCCTGTCCTG CCTGAGCTGG GAACGCCAAC CCGAGAGATA
    CAGCTCCGTC TGGACCACGA GCGGAGATTG AGAGCTGGAG CAGAGGACAG AGAAGGCTTT
    ACTTCATCTA TCACTCAGGA GGAGGAGAGG AGGCGTGCAG AGCTCCGAAG AGGACTCGAG
    CTGCAAGGGC TTCAGTCTCA TGAGCCAACA TGGCCACAGG AAAGAGGCCT GCTGCAACAG
    GAAGTGAGGT TGTTCCGCCA CAACATGGTG ATTGTGTACA TGAAGCTGCG CTGGATCCTC
    ATGCACTGGA GACTCGGACG CAGGACGGAC ACCATGGAGG AGGGAGCACA TGCTGAGTAT
    GAACGACTGG AGAATATTCC AGAACTGGGC ATTATGATCG ATCAGGGTGA CGGAGACACG
    GAGCGGGAAG ACAAAGTGCA TCCTCAAATA ACAGGAAGTG ACATTGCAGA GCCAGGACCA
    CTTCTGCTGC CATCGCCGGA GAGATTCCAG TACCAGAAAC AGGCGTGTGA CAGTCGGCGT
    GTTCTGCATG CGCTACGGTC TCTGCTGGAG GAGTTCAGGG CGGAGCTTCG GGAGGAGGCG
    GGGCGGCGCT GGCAGCTGCA GCAGGCGTTA GCCAATGAGA GAGCGGCATG GGAAATACAG
    CGCGCTGAAA TGAGGAGTCA CATCAACACG CTGCAGGATG CCAGAGGGGT TTCTACAGAT
    GCAGATGAAG GTGCAGTTTC TACAGATGTT TTACAGCTGG AGCGGGAAGA GCAGCGCCGC
    CTTCTGGCCG ACAGTCACAG CACGGCACTG GAACTGCGCT GGAAACTGCA GCACGGAGAA
    AAGCGCTGGA CTCGAGAGCG CAACGAACTT CTGGAGCGAT TTGAGAAAGA GCGGCAGGAA
    TGGGACCGCA GCTTACGGGA CATGCACCGC AAGATGGAAA AGATGCAGAA AGATGCTCTT
    CAGTCAGATG AGGGCGCAGC ACATGTCTTC TCCCCTCAGG AAAGCCCTTG TAAGGCTCCC
    AGATCTCCAC GTTCCCCCTG CATTGCAGCA CACACTCGCT CACACTCCGA CTCCGAGGCC
    ACACTGGAGG AGAAGGGAGC AGTAGGAAGA CCCAGGCCCT GTGAGGGACC CCGGCCCCCA
    GAGAGCCTGT TTCTGGATGC CCTCTCCCTG GACCCCCTGA GCGAAGCTGA GGTTCCCCCT
    CCATCACGTC TGGAGAGTGA AAAAAGGTTC CCCCACCTGA AGGAAGCACT GAATGAGATT
    TCTGAGATTG CGGATCCTGC CATTTACCCT GAAGAAGAGA AAAGTAGTGG CAGCTTACTC
    AGGGCAAAGT CAGTCTGCTC CATGAGCGAC TTCCAACGTT TAATGGACAG CTCTCCCTTT
    CTACCAGACA AAAGTAAGAC TGGTGAGCAT GGGCGGGATG ATGTGACCCC TCCTCTTTCT
    CCAGACGACC TCAAGTACAT TGAGGAGTTT AATAGTAAGG GTTGGGATCT GCCCACCAGC
    TCTCTAGCCA CTTGCCCCAT TCCTGGCCCA GTAATCCCCT CTCCGGTGAT GGAGGCCTGG
    GCGGAGAGAG CAGAATCCCG AAGGGCAGAT TCTACTTCAG AAGCATTCCA GCAAGCCTCT
    TGGTTCTTGA CCACCAGCGC TACCTTGACC ACCAACACCA TGAGTAGCCC TGAGTACTGC
    CAAAAGGTCC AACTCAGGGC AGCTCAGGGC AGTGACCAGT TTGGGGTGCA TGTTCTTCAC
    AGTCCGTCTC GAGGGGAACG ATCCGCTCCG AGTCCTTCTG AGCAGGAATA CATGTTCTCC
    AAAGTGACCA AAGTGAAGGC AGGGGAAGTA GTAGGGGGTG CAGAAGAAGT ATTTGGAGGA
    AAATGGCCGT GTGAGGTTGG GAGTCATTTG GAGGCAGGAC TTAGGCCTGG TGAGCGTCCA
    TCAATTTGTG CTGCAGTCGG CTATACCTCA TCACTAGAGC TGGAGCTGTC ACGCAATCTG
    AGCGATGATA TGAAGGAGAA GGCATATTCC GCCCGCAATG CAATCCGATC ATCTACTACC
    ACTGGATCCA GCCCTCCGGA AAGGCAGCTA CGGGACATGG CATGCCAGAC GAATGGTCTG
    ACCTCACGAG GAACACAGAC CACTCAGACT ATCAGTGTGG GACTGCAAAC TGAGGCTCTT
    CGCACCCTCA CGAGCAGCCC GCATCGCTGT CTTACCCCCA AAGGAGGAGG CTCTACTCCC
    ATCTCATCCC CTTCACGTAG CCTAAGAAAG ATGCAGTACT CTCCAGTCGT CCAAGCCAAG
    TTTGAACGGC CATGCTGTTC TCCAAAATAC GGCTCACCCA AGCTTCAAAG GAAACCTTCT
    AATGCTAGTA AAACAGAACA GCCTGTCAGC CAAACCCGTG CTCCCACCCC AACAACTCCT
    GTCCAGCAGC AAAAAGGCAA TAACGAATCC GCTTGGGCTC GTTCTACGAC CACCAGAGAT
    AGCCCCGTTC ACACCACCAT CAACGACGGC CTCTCCAGCC TCTTCAACAT CATCGACCAC
    ACGCCCATCG CCTACGACCC CATGCAGAAG TTCAACAAAT CCCCAAGCCG CTCTCGTCCT
    ACCGAATCAG GGCCAACCGA GCCCAAATCT CCCAGTGTTA GTGCTGCACA GGGGCCTTTG
    AGGAACTCTC GGGGCCGGTC CCCAAGCCCT GTGCAGCTGA TTGTAGAGAC GCAGGGCGAG
    AAAACTCCAG AGGTGATTAG CATCAGACAG GACCTGTCTG CTCCTCCGGG CTACACGTTA
    GCAGAGAACG CAGCACGGAT ATTGAATAAG AAACTACTGG AGCAGAGCTT CAGGGAGGAA
    AGGAGGCTTT CTGCTTCATC CGCAGCTGTC CCGAACAGAA CCGGAGACTC AGAGAAACCC
    GGATGTCTTG AGGATCTGCC ACGTTCTCCT GTGGCTCCGC CTCTGGACTC CTGCTTTCTG
    CGTCCGGCTC GTCCTGCCAA TCGGCGTCCT CCGTCCCGCT GGGCGTCTCA CTCCCCCTCA
    TCCTCACCCA AATACAGCGA GCGCTCAGAG CGGTTCCTCT TCCCTTCGCC CGTCCGACCC
    ATTCACACCA TCCCAGAGAC TGAGGAGCCA AGCCTGGAGA CCCGCCTGCA ACCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207940.1
    CDS480..6236
    Translation

    Target ORF information:

    RefSeq Version XM_017352451.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio microtubule crosslinking factor 1 (mtcl1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017352451.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGAGAGCT CCAATGGCTC CGACACCAAA CCGCAGAGCC ATCATCCTCA GGTGGAGAAG 
    AAGAGGTTAA ACCGCGCGCC GTCCCCTGCC AGACCCGTCC TCAAGGATGT CCACTCGCGG
    ACGGCCAAAC CGATCGTGCC AAAATCGCCC AAATTCAACA AGCAGCAAGC GCCGGTGACG
    CCTCAGAGCC GCGTCCCGAG ACGCACCGTC CCGGGCGCGA AGGACAAAAC CGCATCAGCA
    CCTGCAAAAA GCAACACCAA GCCCGCCTCA GCGACAAAAA AGCCCGTTAA GGTCACTCAG
    CATGCAGCAT CCGAACCCAA ACCGGCCGGT AAGGCTGCGG AGTCCGCCGC TCCGCAGATC
    ACCAAGAGCA AGGGGAGACC TGCCCCCATA CGCGTGCCCA CCGGACGCGG CGTCTGTCAG
    ACCGACAGCA GCTCGGATTT GTCTGACTGT CCTTCCGAGC CGCTGTCCGA CGAGCAGAGG
    CTGGTCCAGG CCGCCAGCAG CGACGCGGAG TCGGGCAGCG GCTCGGGTTC GAGTGAGCAG
    GCGGCTGCAG GATCACGGAC TGCACCTGCT CCTGCTCCCG TTGCGCGCGC GGACAAACGC
    AAAGATAATA AAGACGCACA GTTTAAACCC GACGGCAAGG AGATGGAGAC GGAGATGGCC
    CAGGACGAGC TGCTAAGGGA GATCGAAGAC CTGCGATCGG AAAATGACTA CCTCAAGGAT
    GAAGTGGATG AGCTGCGAGC AGAGATGGAG GAGATCCGGG ACAGTTACCT GGAAGAGGAG
    GTGTACCAGC TGCAAGATCT GCGCCGGGAA CTGGACCGCT CCAACAAAAA CTGCCGGATC
    CTCCAGTACC GCCTGCGCAA GGCTGAACAG AAGAGCCTGC GTGTAGCACA GACCGGACAT
    GTGGACGGAG AGCTTCTGCG CGGCCTGGAG CAGGACCTCA AGGTGGCCAA AGATGTATCG
    GTGCGTCTGC ACAATGAACT AGAAACGGTG GAAGACAAGC GAACGCGGGC GGAAGATGAA
    AACGAGCTGC TCAGGCAAAA GATCATCGAA ATCGAGATCT CCAAACAGGC CCTGCATAAT
    GAGCTGGAGA GAGCCAAAGA GACTGCTCTG CGGAGGCGAG GCAGTAGAGA AGCCTTCAAA
    GATAAGAAGC CTTTATCTCA GGAGGACAGT GCAGATCTGC GCTGTCAGCT ACAGTTTGCC
    AAGGAGGAAT CGACTCTCAT GCGTAAAAAG ATGGCCAAAC TTGGCCGCGA GAAGGATGAA
    CTGGAACAAG AATTGCAGAA GTATAAATCA GTGTACGGCG ACGTGGACAG CCCTTTGCCT
    TTAGCGGAGG TGACCGGCGG AGGCCCACAC ACCACACGCG AAGCTGAGCT ACGACTACGA
    CTTAAACTAG TAGAAGAAGA AGCAAACATC CTGGGGAGGA AGATTGTGGA GCTGGAAGTT
    GAAAACCGCG GTCTCAGAGC GGAAAATGAA GACTTGCGTA GTCAATATGA AAGGGATTGC
    TTTGGCCGTG AACCCTTCTC CAGCGTCCCT ACCTCACCGT TTGGCGGCGA TGCGCTTGAG
    TCTGCGAGTG AACTGCGCAG ACATTTGCAG TTTGTGGAGG AAGAAGCTGA GCTCCTGCGG
    CGCTCCATTT CTGAAATCGA AGATCACAAC AAACAGCTAA CATCCGAGCT CAACCGCTTC
    AAATTCGGTC CCAATCAATC CGAGCGGGAA TCCGAAGGAG CTGAAGGAGG ACTCAAAATT
    GTGAATACCG CAGGTAATGG TTTTTCAAGC AGCACACTTC AGGATGAACT AAAAAACGCA
    AGACTCCAAG TCAACGAGTT AAGCGGGAAA GTAATGAAGC TTCAATATGA GAATCGGGTG
    CTAATCTCTA ATATGCAGCG CTGTGATTTG GCCGCACACT TGGGAATGCG TACTGGTAGT
    CCACGGGATA GTGATGTAGA CAGCGACGCT GGCCGAAGGG AACCAGATGA AGATGAAACA
    GCAAGGATAT TGCTGCTCCA TCCCAAACGT GAAGGTCCTG TTGGCGGAGA GAGCGATTCT
    GATGATTTAT TTGAACGTAC GGCTACTTCA GGATTCGCCA GTGGTGAAAA ACCTTCAGAT
    TCTGGCGAAC TGGGAGTAAT CGAATTAGCT CAGCGTAGAC GGGAAGACCG GGAAGCGTTT
    AATAATATCA GAAGGGAAGC TGAGAGGCTC GGGAAAACAG TAGAGCGGCT CATAACGGAT
    ACGGATAGTC TTATCCGGGA AGGTCGACTG GTTGTTTTGG GAGGAGATCT TCTTGCGGAG
    GGTGTGGAAT TTAGAGGCGA CGGAGACTCA GCTGAATCTA AACAAGACTC TCAGGTGCTG
    GACTCCATTA ATGCACGCAT GAGGACTTTC CGAACCGATC TCCAGCAATT CATGGATAAA
    GTGGAGCATC TAGGGGATGG TTTACGGGAT CGGGTTGATG ATCTTTCCCC GATGCCTAAC
    CTCACAGAAT CTAGCAGCTT CCTTTCTACG GTTACCTCCA TGTCCAGGGA TTCGCCAATC
    GGGACACTGG GAAGGGATCT AGTGACGGAC TTCCAGTCCG GGCGCAGGGA GGAGCAGGAG
    AGCTGTGTAG ACCGGCCCAG AGGAGCCGCG GGACTCTCTA GATACACCAG ACCACACACT
    TTCAGATCCG AGTTTCGCGA CCCTGTCCTG CCTGAGCTGG GAACGCCAAC CCGAGAGATA
    CAGCTCCGTC TGGACCACGA GCGGAGATTG AGAGCTGGAG CAGAGGACAG AGAAGGCTTT
    ACTTCATCTA TCACTCAGGA GGAGGAGAGG AGGCGTGCAG AGCTCCGAAG AGGACTCGAG
    CTGCAAGGGC TTCAGTCTCA TGAGCCAACA TGGCCACAGG AAAGAGGCCT GCTGCAACAG
    GAAGTGAGGT TGTTCCGCCA CAACATGGTG ATTGTGTACA TGAAGCTGCG CTGGATCCTC
    ATGCACTGGA GACTCGGACG CAGGACGGAC ACCATGGAGG AGGGAGCACA TGCTGAGTAT
    GAACGACTGG AGAATATTCC AGAACTGGGC ATTATGATCG ATCAGGGTGA CGGAGACACG
    GAGCGGGAAG ACAAAGTGCA TCCTCAAATA ACAGGAAGTG ACATTGCAGA GCCAGGACCA
    CTTCTGCTGC CATCGCCGGA GAGATTCCAG TACCAGAAAC AGGCGTGTGA CAGTCGGCGT
    GTTCTGCATG CGCTACGGTC TCTGCTGGAG GAGTTCAGGG CGGAGCTTCG GGAGGAGGCG
    GGGCGGCGCT GGCAGCTGCA GCAGGCGTTA GCCAATGAGA GAGCGGCATG GGAAATACAG
    CGCGCTGAAA TGAGGAGTCA CATCAACACG CTGCAGGATG CCAGAGGGGT TTCTACAGAT
    GCAGATGAAG GTGCAGTTTC TACAGATGTT TTACAGCTGG AGCGGGAAGA GCAGCGCCGC
    CTTCTGGCCG ACAGTCACAG CACGGCACTG GAACTGCGCT GGAAACTGCA GCACGGAGAA
    AAGCGCTGGA CTCGAGAGCG CAACGAACTT CTGGAGCGAT TTGAGAAAGA GCGGCAGGAA
    TGGGACCGCA GCTTACGGGA CATGCACCGC AAGATGGAAA AGATGCAGAA AGATGCTCTT
    CAGTCAGATG AGGGCGCAGC ACATGTCTTC TCCCCTCAGG AAAGCCCTTG TAAGGCTCCC
    AGATCTCCAC GTTCCCCCTG CATTGCAGCA CACACTCGCT CACACTCCGA CTCCGAGGCC
    ACACTGGAGG AGAAGGGAGC AGTAGGAAGA CCCAGGCCCT GTGAGGGACC CCGGCCCCCA
    GAGAGCCTGT TTCTGGATGC CCTCTCCCTG GACCCCCTGA GCGAAGCTGA GGTTCCCCCT
    CCATCACGTC TGGAGAGTGA AAAAAGGTTC CCCCACCTGA AGGAAGCACT GAATGAGATT
    TCTGAGATTG CGGATCCTGC CATTTACCCT GAAGAAGAGA AAAGTAGTGG CAGCTTACTC
    AGGGCAAAGT CAGTCTGCTC CATGAGCGAC TTCCAACGTT TAATGGACAG CTCTCCCTTT
    CTACCAGACA AAAGTAAGAC TGGTGAGCAT GGGCGGGATG ATGTGACCCC TCCTCTTTCT
    CCAGACGACC TCAAGTACAT TGAGGAGTTT AATAGTAAGG GTTGGGATCT GCCCACCAGC
    TCTCTAGCCA CTTGCCCCAT TCCTGGCCCA GTAATCCCCT CTCCGGTGAT GGAGGCCTGG
    GCGGAGAGAG CAGAATCCCG AAGGGCAGAT TCTACTTCAG AAGCATTCCA GCAAGCCTCT
    TGGTTCTTGA CCACCAGCGC TACCTTGACC ACCAACACCA TGAGTAGCCC TGAGTACTGC
    CAAAAGGTCC AACTCAGGGC AGCTCAGGGC AGTGACCAGT TTGGGGTGCA TGTTCTTCAC
    AGTCCGTCTC GAGGGGAACG ATCCGCTCCG AGTCCTTCTG AGCAGGAATA CATGTTCTCC
    AAAGTGACCA AAGTGAAGGC AGGGGAAGTA GTAGGGGGTG CAGAAGAAGT ATTTGGAGGA
    AAATGGCCGT GTGAGGTTGG GAGTCATTTG GAGGCAGGAC TTAGGCCTGG TGAGCGTCCA
    TCAATTTGTG CTGCAGTCGG CTATACCTCA TCACTAGAGC TGGAGCTGTC ACGCAATCTG
    AGCGATGATA TGAAGGAGAA GGCATATTCC GCCCGCAATG CAATCCGATC ATCTACTACC
    ACTGGATCCA GCCCTCCGGA AAGGCAGCTA CGGGACATGG CATGCCAGAC GAATGGTCTG
    ACCTCACGAG GAACACAGAC CACTCAGACT ATCAGTGTGG GACTGCAAAC TGAGGCTCTT
    CGCACCCTCA CGAGCAGCCC GCATCGCTGT CTTACCCCCA AAGGAGGAGG CTCTACTCCC
    ATCTCATCCC CTTCACGTAG CCTAAGAAAG ATGCAGTACT CTCCAGTCGT CCAAGCCAAG
    TTTGAACGGC CATGCTGTTC TCCAAAATAC GGCTCACCCA AGCTTCAAAG GAAACCTTCT
    AATGCTAGTA AAACAGAACA GCCTGTCAGC CAAACCCGTG CTCCCACCCC AACAACTCCT
    GTCCAGCAGC AAAAAGGCAA TAACGAATCC GCTTGGGCTC GTTCTACGAC CACCAGAGAT
    AGCCCCGTTC ACACCACCAT CAACGACGGC CTCTCCAGCC TCTTCAACAT CATCGACCAC
    ACGCCCATCG CCTACGACCC CATGCAGAAG TTCAACAAAT CCCCAAGCCG CTCTCGTCCT
    ACCGAATCAG GGCCAACCGA GCCCAAATCT CCCAGTGTTA GTGCTGCACA GGGGCCTTTG
    AGGAACTCTC GGGGCCGGTC CCCAAGCCCT GTGCAGCTGA TTGTAGAGAC GCAGGGCGAG
    AAAACTCCAG AGGTGATTAG CATCAGACAG GACCTGTCTG CTCCTCCGGG CTACACGTTA
    GCAGAGAACG CAGCACGGAT ATTGAATAAG AAACTACTGG AGCAGAGCTT CAGGGAGGAA
    AGGAGGCTTT CTGCTTCATC CGCAGCTGTC CCGAACAGAA CCGGAGACTC AGAGAAACCC
    GGATGTCTTG AGGATCTGCC ACGTTCTCCT GTGGCTCCGC CTCTGGACTC CTGCTTTCTG
    CGTCCGGCTC GTCCTGCCAA TCGGCGTCCT CCGTCCCGCT GGGCGTCTCA CTCCCCCTCA
    TCCTCACCCA AATACAGCGA GCGCTCAGAG CGGTTCCTCT TCCCTTCGCC CGTCCGACCC
    ATTCACACCA TCCCAGAGAC TGAGGAGCCA AGCCTGGAGA CCCGCCTGCA ACCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa56508
    Clone ID Related Accession (Same CDS sequence) XM_017352451.2 , XM_017352451.1
    Accession Version XM_017352451.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5757bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product microtubule cross-linking factor 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGAGAGCT CCAATGGCTC CGACACCAAA CCGCAGAGCC ATCATCCTCA GGTGGAGAAG 
    AAGAGGTTAA ACCGCGCGCC GTCCCCTGCC AGACCCGTCC TCAAGGATGT CCACTCGCGG
    ACGGCCAAAC CGATCGTGCC AAAATCGCCC AAATTCAACA AGCAGCAAGC GCCGGTGACG
    CCTCAGAGCC GCGTCCCGAG ACGCACCGTC CCGGGCGCGA AGGACAAAAC CGCATCAGCA
    CCTGCAAAAA GCAACACCAA GCCCGCCTCA GCGACAAAAA AGCCCGTTAA GGTCACTCAG
    CATGCAGCAT CCGAACCCAA ACCGGCCGGT AAGGCTGCGG AGTCCGCCGC TCCGCAGATC
    ACCAAGAGCA AGGGGAGACC TGCCCCCATA CGCGTGCCCA CCGGACGCGG CGTCTGTCAG
    ACCGACAGCA GCTCGGATTT GTCTGACTGT CCTTCCGAGC CGCTGTCCGA CGAGCAGAGG
    CTGGTCCAGG CCGCCAGCAG CGACGCGGAG TCGGGCAGCG GCTCGGGTTC GAGTGAGCAG
    GCGGCTGCAG GATCACGGAC TGCACCTGCT CCTGCTCCCG TTGCGCGCGC GGACAAACGC
    AAAGATAATA AAGACGCACA GTTTAAACCC GACGGCAAGG AGATGGAGAC GGAGATGGCC
    CAGGACGAGC TGCTAAGGGA GATCGAAGAC CTGCGATCGG AAAATGACTA CCTCAAGGAT
    GAAGTGGATG AGCTGCGAGC AGAGATGGAG GAGATCCGGG ACAGTTACCT GGAAGAGGAG
    GTGTACCAGC TGCAAGATCT GCGCCGGGAA CTGGACCGCT CCAACAAAAA CTGCCGGATC
    CTCCAGTACC GCCTGCGCAA GGCTGAACAG AAGAGCCTGC GTGTAGCACA GACCGGACAT
    GTGGACGGAG AGCTTCTGCG CGGCCTGGAG CAGGACCTCA AGGTGGCCAA AGATGTATCG
    GTGCGTCTGC ACAATGAACT AGAAACGGTG GAAGACAAGC GAACGCGGGC GGAAGATGAA
    AACGAGCTGC TCAGGCAAAA GATCATCGAA ATCGAGATCT CCAAACAGGC CCTGCATAAT
    GAGCTGGAGA GAGCCAAAGA GACTGCTCTG CGGAGGCGAG GCAGTAGAGA AGCCTTCAAA
    GATAAGAAGC CTTTATCTCA GGAGGACAGT GCAGATCTGC GCTGTCAGCT ACAGTTTGCC
    AAGGAGGAAT CGACTCTCAT GCGTAAAAAG ATGGCCAAAC TTGGCCGCGA GAAGGATGAA
    CTGGAACAAG AATTGCAGAA GTATAAATCA GTGTACGGCG ACGTGGACAG CCCTTTGCCT
    TTAGCGGAGG TGACCGGCGG AGGCCCACAC ACCACACGCG AAGCTGAGCT ACGACTACGA
    CTTAAACTAG TAGAAGAAGA AGCAAACATC CTGGGGAGGA AGATTGTGGA GCTGGAAGTT
    GAAAACCGCG GTCTCAGAGC GGAAAATGAA GACTTGCGTA GTCAATATGA AAGGGATTGC
    TTTGGCCGTG AACCCTTCTC CAGCGTCCCT ACCTCACCGT TTGGCGGCGA TGCGCTTGAG
    TCTGCGAGTG AACTGCGCAG ACATTTGCAG TTTGTGGAGG AAGAAGCTGA GCTCCTGCGG
    CGCTCCATTT CTGAAATCGA AGATCACAAC AAACAGCTAA CATCCGAGCT CAACCGCTTC
    AAATTCGGTC CCAATCAATC CGAGCGGGAA TCCGAAGGAG CTGAAGGAGG ACTCAAAATT
    GTGAATACCG CAGGTAATGG TTTTTCAAGC AGCACACTTC AGGATGAACT AAAAAACGCA
    AGACTCCAAG TCAACGAGTT AAGCGGGAAA GTAATGAAGC TTCAATATGA GAATCGGGTG
    CTAATCTCTA ATATGCAGCG CTGTGATTTG GCCGCACACT TGGGAATGCG TACTGGTAGT
    CCACGGGATA GTGATGTAGA CAGCGACGCT GGCCGAAGGG AACCAGATGA AGATGAAACA
    GCAAGGATAT TGCTGCTCCA TCCCAAACGT GAAGGTCCTG TTGGCGGAGA GAGCGATTCT
    GATGATTTAT TTGAACGTAC GGCTACTTCA GGATTCGCCA GTGGTGAAAA ACCTTCAGAT
    TCTGGCGAAC TGGGAGTAAT CGAATTAGCT CAGCGTAGAC GGGAAGACCG GGAAGCGTTT
    AATAATATCA GAAGGGAAGC TGAGAGGCTC GGGAAAACAG TAGAGCGGCT CATAACGGAT
    ACGGATAGTC TTATCCGGGA AGGTCGACTG GTTGTTTTGG GAGGAGATCT TCTTGCGGAG
    GGTGTGGAAT TTAGAGGCGA CGGAGACTCA GCTGAATCTA AACAAGACTC TCAGGTGCTG
    GACTCCATTA ATGCACGCAT GAGGACTTTC CGAACCGATC TCCAGCAATT CATGGATAAA
    GTGGAGCATC TAGGGGATGG TTTACGGGAT CGGGTTGATG ATCTTTCCCC GATGCCTAAC
    CTCACAGAAT CTAGCAGCTT CCTTTCTACG GTTACCTCCA TGTCCAGGGA TTCGCCAATC
    GGGACACTGG GAAGGGATCT AGTGACGGAC TTCCAGTCCG GGCGCAGGGA GGAGCAGGAG
    AGCTGTGTAG ACCGGCCCAG AGGAGCCGCG GGACTCTCTA GATACACCAG ACCACACACT
    TTCAGATCCG AGTTTCGCGA CCCTGTCCTG CCTGAGCTGG GAACGCCAAC CCGAGAGATA
    CAGCTCCGTC TGGACCACGA GCGGAGATTG AGAGCTGGAG CAGAGGACAG AGAAGGCTTT
    ACTTCATCTA TCACTCAGGA GGAGGAGAGG AGGCGTGCAG AGCTCCGAAG AGGACTCGAG
    CTGCAAGGGC TTCAGTCTCA TGAGCCAACA TGGCCACAGG AAAGAGGCCT GCTGCAACAG
    GAAGTGAGGT TGTTCCGCCA CAACATGGTG ATTGTGTACA TGAAGCTGCG CTGGATCCTC
    ATGCACTGGA GACTCGGACG CAGGACGGAC ACCATGGAGG AGGGAGCACA TGCTGAGTAT
    GAACGACTGG AGAATATTCC AGAACTGGGC ATTATGATCG ATCAGGGTGA CGGAGACACG
    GAGCGGGAAG ACAAAGTGCA TCCTCAAATA ACAGGAAGTG ACATTGCAGA GCCAGGACCA
    CTTCTGCTGC CATCGCCGGA GAGATTCCAG TACCAGAAAC AGGCGTGTGA CAGTCGGCGT
    GTTCTGCATG CGCTACGGTC TCTGCTGGAG GAGTTCAGGG CGGAGCTTCG GGAGGAGGCG
    GGGCGGCGCT GGCAGCTGCA GCAGGCGTTA GCCAATGAGA GAGCGGCATG GGAAATACAG
    CGCGCTGAAA TGAGGAGTCA CATCAACACG CTGCAGGATG CCAGAGGGGT TTCTACAGAT
    GCAGATGAAG GTGCAGTTTC TACAGATGTT TTACAGCTGG AGCGGGAAGA GCAGCGCCGC
    CTTCTGGCCG ACAGTCACAG CACGGCACTG GAACTGCGCT GGAAACTGCA GCACGGAGAA
    AAGCGCTGGA CTCGAGAGCG CAACGAACTT CTGGAGCGAT TTGAGAAAGA GCGGCAGGAA
    TGGGACCGCA GCTTACGGGA CATGCACCGC AAGATGGAAA AGATGCAGAA AGATGCTCTT
    CAGTCAGATG AGGGCGCAGC ACATGTCTTC TCCCCTCAGG AAAGCCCTTG TAAGGCTCCC
    AGATCTCCAC GTTCCCCCTG CATTGCAGCA CACACTCGCT CACACTCCGA CTCCGAGGCC
    ACACTGGAGG AGAAGGGAGC AGTAGGAAGA CCCAGGCCCT GTGAGGGACC CCGGCCCCCA
    GAGAGCCTGT TTCTGGATGC CCTCTCCCTG GACCCCCTGA GCGAAGCTGA GGTTCCCCCT
    CCATCACGTC TGGAGAGTGA AAAAAGGTTC CCCCACCTGA AGGAAGCACT GAATGAGATT
    TCTGAGATTG CGGATCCTGC CATTTACCCT GAAGAAGAGA AAAGTAGTGG CAGCTTACTC
    AGGGCAAAGT CAGTCTGCTC CATGAGCGAC TTCCAACGTT TAATGGACAG CTCTCCCTTT
    CTACCAGACA AAAGTAAGAC TGGTGAGCAT GGGCGGGATG ATGTGACCCC TCCTCTTTCT
    CCAGACGACC TCAAGTACAT TGAGGAGTTT AATAGTAAGG GTTGGGATCT GCCCACCAGC
    TCTCTAGCCA CTTGCCCCAT TCCTGGCCCA GTAATCCCCT CTCCGGTGAT GGAGGCCTGG
    GCGGAGAGAG CAGAATCCCG AAGGGCAGAT TCTACTTCAG AAGCATTCCA GCAAGCCTCT
    TGGTTCTTGA CCACCAGCGC TACCTTGACC ACCAACACCA TGAGTAGCCC TGAGTACTGC
    CAAAAGGTCC AACTCAGGGC AGCTCAGGGC AGTGACCAGT TTGGGGTGCA TGTTCTTCAC
    AGTCCGTCTC GAGGGGAACG ATCCGCTCCG AGTCCTTCTG AGCAGGAATA CATGTTCTCC
    AAAGTGACCA AAGTGAAGGC AGGGGAAGTA GTAGGGGGTG CAGAAGAAGT ATTTGGAGGA
    AAATGGCCGT GTGAGGTTGG GAGTCATTTG GAGGCAGGAC TTAGGCCTGG TGAGCGTCCA
    TCAATTTGTG CTGCAGTCGG CTATACCTCA TCACTAGAGC TGGAGCTGTC ACGCAATCTG
    AGCGATGATA TGAAGGAGAA GGCATATTCC GCCCGCAATG CAATCCGATC ATCTACTACC
    ACTGGATCCA GCCCTCCGGA AAGGCAGCTA CGGGACATGG CATGCCAGAC GAATGGTCTG
    ACCTCACGAG GAACACAGAC CACTCAGACT ATCAGTGTGG GACTGCAAAC TGAGGCTCTT
    CGCACCCTCA CGAGCAGCCC GCATCGCTGT CTTACCCCCA AAGGAGGAGG CTCTACTCCC
    ATCTCATCCC CTTCACGTAG CCTAAGAAAG ATGCAGTACT CTCCAGTCGT CCAAGCCAAG
    TTTGAACGGC CATGCTGTTC TCCAAAATAC GGCTCACCCA AGCTTCAAAG GAAACCTTCT
    AATGCTAGTA AAACAGAACA GCCTGTCAGC CAAACCCGTG CTCCCACCCC AACAACTCCT
    GTCCAGCAGC AAAAAGGCAA TAACGAATCC GCTTGGGCTC GTTCTACGAC CACCAGAGAT
    AGCCCCGTTC ACACCACCAT CAACGACGGC CTCTCCAGCC TCTTCAACAT CATCGACCAC
    ACGCCCATCG CCTACGACCC CATGCAGAAG TTCAACAAAT CCCCAAGCCG CTCTCGTCCT
    ACCGAATCAG GGCCAACCGA GCCCAAATCT CCCAGTGTTA GTGCTGCACA GGGGCCTTTG
    AGGAACTCTC GGGGCCGGTC CCCAAGCCCT GTGCAGCTGA TTGTAGAGAC GCAGGGCGAG
    AAAACTCCAG AGGTGATTAG CATCAGACAG GACCTGTCTG CTCCTCCGGG CTACACGTTA
    GCAGAGAACG CAGCACGGAT ATTGAATAAG AAACTACTGG AGCAGAGCTT CAGGGAGGAA
    AGGAGGCTTT CTGCTTCATC CGCAGCTGTC CCGAACAGAA CCGGAGACTC AGAGAAACCC
    GGATGTCTTG AGGATCTGCC ACGTTCTCCT GTGGCTCCGC CTCTGGACTC CTGCTTTCTG
    CGTCCGGCTC GTCCTGCCAA TCGGCGTCCT CCGTCCCGCT GGGCGTCTCA CTCCCCCTCA
    TCCTCACCCA AATACAGCGA GCGCTCAGAG CGGTTCCTCT TCCCTTCGCC CGTCCGACCC
    ATTCACACCA TCCCAGAGAC TGAGGAGCCA AGCCTGGAGA CCCGCCTGCA ACCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207940.1
    CDS510..6266
    Translation

    Target ORF information:

    RefSeq Version XM_017352451.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio microtubule crosslinking factor 1 (mtcl1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017352451.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGAGAGCT CCAATGGCTC CGACACCAAA CCGCAGAGCC ATCATCCTCA GGTGGAGAAG 
    AAGAGGTTAA ACCGCGCGCC GTCCCCTGCC AGACCCGTCC TCAAGGATGT CCACTCGCGG
    ACGGCCAAAC CGATCGTGCC AAAATCGCCC AAATTCAACA AGCAGCAAGC GCCGGTGACG
    CCTCAGAGCC GCGTCCCGAG ACGCACCGTC CCGGGCGCGA AGGACAAAAC CGCATCAGCA
    CCTGCAAAAA GCAACACCAA GCCCGCCTCA GCGACAAAAA AGCCCGTTAA GGTCACTCAG
    CATGCAGCAT CCGAACCCAA ACCGGCCGGT AAGGCTGCGG AGTCCGCCGC TCCGCAGATC
    ACCAAGAGCA AGGGGAGACC TGCCCCCATA CGCGTGCCCA CCGGACGCGG CGTCTGTCAG
    ACCGACAGCA GCTCGGATTT GTCTGACTGT CCTTCCGAGC CGCTGTCCGA CGAGCAGAGG
    CTGGTCCAGG CCGCCAGCAG CGACGCGGAG TCGGGCAGCG GCTCGGGTTC GAGTGAGCAG
    GCGGCTGCAG GATCACGGAC TGCACCTGCT CCTGCTCCCG TTGCGCGCGC GGACAAACGC
    AAAGATAATA AAGACGCACA GTTTAAACCC GACGGCAAGG AGATGGAGAC GGAGATGGCC
    CAGGACGAGC TGCTAAGGGA GATCGAAGAC CTGCGATCGG AAAATGACTA CCTCAAGGAT
    GAAGTGGATG AGCTGCGAGC AGAGATGGAG GAGATCCGGG ACAGTTACCT GGAAGAGGAG
    GTGTACCAGC TGCAAGATCT GCGCCGGGAA CTGGACCGCT CCAACAAAAA CTGCCGGATC
    CTCCAGTACC GCCTGCGCAA GGCTGAACAG AAGAGCCTGC GTGTAGCACA GACCGGACAT
    GTGGACGGAG AGCTTCTGCG CGGCCTGGAG CAGGACCTCA AGGTGGCCAA AGATGTATCG
    GTGCGTCTGC ACAATGAACT AGAAACGGTG GAAGACAAGC GAACGCGGGC GGAAGATGAA
    AACGAGCTGC TCAGGCAAAA GATCATCGAA ATCGAGATCT CCAAACAGGC CCTGCATAAT
    GAGCTGGAGA GAGCCAAAGA GACTGCTCTG CGGAGGCGAG GCAGTAGAGA AGCCTTCAAA
    GATAAGAAGC CTTTATCTCA GGAGGACAGT GCAGATCTGC GCTGTCAGCT ACAGTTTGCC
    AAGGAGGAAT CGACTCTCAT GCGTAAAAAG ATGGCCAAAC TTGGCCGCGA GAAGGATGAA
    CTGGAACAAG AATTGCAGAA GTATAAATCA GTGTACGGCG ACGTGGACAG CCCTTTGCCT
    TTAGCGGAGG TGACCGGCGG AGGCCCACAC ACCACACGCG AAGCTGAGCT ACGACTACGA
    CTTAAACTAG TAGAAGAAGA AGCAAACATC CTGGGGAGGA AGATTGTGGA GCTGGAAGTT
    GAAAACCGCG GTCTCAGAGC GGAAAATGAA GACTTGCGTA GTCAATATGA AAGGGATTGC
    TTTGGCCGTG AACCCTTCTC CAGCGTCCCT ACCTCACCGT TTGGCGGCGA TGCGCTTGAG
    TCTGCGAGTG AACTGCGCAG ACATTTGCAG TTTGTGGAGG AAGAAGCTGA GCTCCTGCGG
    CGCTCCATTT CTGAAATCGA AGATCACAAC AAACAGCTAA CATCCGAGCT CAACCGCTTC
    AAATTCGGTC CCAATCAATC CGAGCGGGAA TCCGAAGGAG CTGAAGGAGG ACTCAAAATT
    GTGAATACCG CAGGTAATGG TTTTTCAAGC AGCACACTTC AGGATGAACT AAAAAACGCA
    AGACTCCAAG TCAACGAGTT AAGCGGGAAA GTAATGAAGC TTCAATATGA GAATCGGGTG
    CTAATCTCTA ATATGCAGCG CTGTGATTTG GCCGCACACT TGGGAATGCG TACTGGTAGT
    CCACGGGATA GTGATGTAGA CAGCGACGCT GGCCGAAGGG AACCAGATGA AGATGAAACA
    GCAAGGATAT TGCTGCTCCA TCCCAAACGT GAAGGTCCTG TTGGCGGAGA GAGCGATTCT
    GATGATTTAT TTGAACGTAC GGCTACTTCA GGATTCGCCA GTGGTGAAAA ACCTTCAGAT
    TCTGGCGAAC TGGGAGTAAT CGAATTAGCT CAGCGTAGAC GGGAAGACCG GGAAGCGTTT
    AATAATATCA GAAGGGAAGC TGAGAGGCTC GGGAAAACAG TAGAGCGGCT CATAACGGAT
    ACGGATAGTC TTATCCGGGA AGGTCGACTG GTTGTTTTGG GAGGAGATCT TCTTGCGGAG
    GGTGTGGAAT TTAGAGGCGA CGGAGACTCA GCTGAATCTA AACAAGACTC TCAGGTGCTG
    GACTCCATTA ATGCACGCAT GAGGACTTTC CGAACCGATC TCCAGCAATT CATGGATAAA
    GTGGAGCATC TAGGGGATGG TTTACGGGAT CGGGTTGATG ATCTTTCCCC GATGCCTAAC
    CTCACAGAAT CTAGCAGCTT CCTTTCTACG GTTACCTCCA TGTCCAGGGA TTCGCCAATC
    GGGACACTGG GAAGGGATCT AGTGACGGAC TTCCAGTCCG GGCGCAGGGA GGAGCAGGAG
    AGCTGTGTAG ACCGGCCCAG AGGAGCCGCG GGACTCTCTA GATACACCAG ACCACACACT
    TTCAGATCCG AGTTTCGCGA CCCTGTCCTG CCTGAGCTGG GAACGCCAAC CCGAGAGATA
    CAGCTCCGTC TGGACCACGA GCGGAGATTG AGAGCTGGAG CAGAGGACAG AGAAGGCTTT
    ACTTCATCTA TCACTCAGGA GGAGGAGAGG AGGCGTGCAG AGCTCCGAAG AGGACTCGAG
    CTGCAAGGGC TTCAGTCTCA TGAGCCAACA TGGCCACAGG AAAGAGGCCT GCTGCAACAG
    GAAGTGAGGT TGTTCCGCCA CAACATGGTG ATTGTGTACA TGAAGCTGCG CTGGATCCTC
    ATGCACTGGA GACTCGGACG CAGGACGGAC ACCATGGAGG AGGGAGCACA TGCTGAGTAT
    GAACGACTGG AGAATATTCC AGAACTGGGC ATTATGATCG ATCAGGGTGA CGGAGACACG
    GAGCGGGAAG ACAAAGTGCA TCCTCAAATA ACAGGAAGTG ACATTGCAGA GCCAGGACCA
    CTTCTGCTGC CATCGCCGGA GAGATTCCAG TACCAGAAAC AGGCGTGTGA CAGTCGGCGT
    GTTCTGCATG CGCTACGGTC TCTGCTGGAG GAGTTCAGGG CGGAGCTTCG GGAGGAGGCG
    GGGCGGCGCT GGCAGCTGCA GCAGGCGTTA GCCAATGAGA GAGCGGCATG GGAAATACAG
    CGCGCTGAAA TGAGGAGTCA CATCAACACG CTGCAGGATG CCAGAGGGGT TTCTACAGAT
    GCAGATGAAG GTGCAGTTTC TACAGATGTT TTACAGCTGG AGCGGGAAGA GCAGCGCCGC
    CTTCTGGCCG ACAGTCACAG CACGGCACTG GAACTGCGCT GGAAACTGCA GCACGGAGAA
    AAGCGCTGGA CTCGAGAGCG CAACGAACTT CTGGAGCGAT TTGAGAAAGA GCGGCAGGAA
    TGGGACCGCA GCTTACGGGA CATGCACCGC AAGATGGAAA AGATGCAGAA AGATGCTCTT
    CAGTCAGATG AGGGCGCAGC ACATGTCTTC TCCCCTCAGG AAAGCCCTTG TAAGGCTCCC
    AGATCTCCAC GTTCCCCCTG CATTGCAGCA CACACTCGCT CACACTCCGA CTCCGAGGCC
    ACACTGGAGG AGAAGGGAGC AGTAGGAAGA CCCAGGCCCT GTGAGGGACC CCGGCCCCCA
    GAGAGCCTGT TTCTGGATGC CCTCTCCCTG GACCCCCTGA GCGAAGCTGA GGTTCCCCCT
    CCATCACGTC TGGAGAGTGA AAAAAGGTTC CCCCACCTGA AGGAAGCACT GAATGAGATT
    TCTGAGATTG CGGATCCTGC CATTTACCCT GAAGAAGAGA AAAGTAGTGG CAGCTTACTC
    AGGGCAAAGT CAGTCTGCTC CATGAGCGAC TTCCAACGTT TAATGGACAG CTCTCCCTTT
    CTACCAGACA AAAGTAAGAC TGGTGAGCAT GGGCGGGATG ATGTGACCCC TCCTCTTTCT
    CCAGACGACC TCAAGTACAT TGAGGAGTTT AATAGTAAGG GTTGGGATCT GCCCACCAGC
    TCTCTAGCCA CTTGCCCCAT TCCTGGCCCA GTAATCCCCT CTCCGGTGAT GGAGGCCTGG
    GCGGAGAGAG CAGAATCCCG AAGGGCAGAT TCTACTTCAG AAGCATTCCA GCAAGCCTCT
    TGGTTCTTGA CCACCAGCGC TACCTTGACC ACCAACACCA TGAGTAGCCC TGAGTACTGC
    CAAAAGGTCC AACTCAGGGC AGCTCAGGGC AGTGACCAGT TTGGGGTGCA TGTTCTTCAC
    AGTCCGTCTC GAGGGGAACG ATCCGCTCCG AGTCCTTCTG AGCAGGAATA CATGTTCTCC
    AAAGTGACCA AAGTGAAGGC AGGGGAAGTA GTAGGGGGTG CAGAAGAAGT ATTTGGAGGA
    AAATGGCCGT GTGAGGTTGG GAGTCATTTG GAGGCAGGAC TTAGGCCTGG TGAGCGTCCA
    TCAATTTGTG CTGCAGTCGG CTATACCTCA TCACTAGAGC TGGAGCTGTC ACGCAATCTG
    AGCGATGATA TGAAGGAGAA GGCATATTCC GCCCGCAATG CAATCCGATC ATCTACTACC
    ACTGGATCCA GCCCTCCGGA AAGGCAGCTA CGGGACATGG CATGCCAGAC GAATGGTCTG
    ACCTCACGAG GAACACAGAC CACTCAGACT ATCAGTGTGG GACTGCAAAC TGAGGCTCTT
    CGCACCCTCA CGAGCAGCCC GCATCGCTGT CTTACCCCCA AAGGAGGAGG CTCTACTCCC
    ATCTCATCCC CTTCACGTAG CCTAAGAAAG ATGCAGTACT CTCCAGTCGT CCAAGCCAAG
    TTTGAACGGC CATGCTGTTC TCCAAAATAC GGCTCACCCA AGCTTCAAAG GAAACCTTCT
    AATGCTAGTA AAACAGAACA GCCTGTCAGC CAAACCCGTG CTCCCACCCC AACAACTCCT
    GTCCAGCAGC AAAAAGGCAA TAACGAATCC GCTTGGGCTC GTTCTACGAC CACCAGAGAT
    AGCCCCGTTC ACACCACCAT CAACGACGGC CTCTCCAGCC TCTTCAACAT CATCGACCAC
    ACGCCCATCG CCTACGACCC CATGCAGAAG TTCAACAAAT CCCCAAGCCG CTCTCGTCCT
    ACCGAATCAG GGCCAACCGA GCCCAAATCT CCCAGTGTTA GTGCTGCACA GGGGCCTTTG
    AGGAACTCTC GGGGCCGGTC CCCAAGCCCT GTGCAGCTGA TTGTAGAGAC GCAGGGCGAG
    AAAACTCCAG AGGTGATTAG CATCAGACAG GACCTGTCTG CTCCTCCGGG CTACACGTTA
    GCAGAGAACG CAGCACGGAT ATTGAATAAG AAACTACTGG AGCAGAGCTT CAGGGAGGAA
    AGGAGGCTTT CTGCTTCATC CGCAGCTGTC CCGAACAGAA CCGGAGACTC AGAGAAACCC
    GGATGTCTTG AGGATCTGCC ACGTTCTCCT GTGGCTCCGC CTCTGGACTC CTGCTTTCTG
    CGTCCGGCTC GTCCTGCCAA TCGGCGTCCT CCGTCCCGCT GGGCGTCTCA CTCCCCCTCA
    TCCTCACCCA AATACAGCGA GCGCTCAGAG CGGTTCCTCT TCCCTTCGCC CGTCCGACCC
    ATTCACACCA TCCCAGAGAC TGAGGAGCCA AGCCTGGAGA CCCGCCTGCA ACCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa56509
    Clone ID Related Accession (Same CDS sequence) XM_017352452.2 , XM_017352452.1
    Accession Version XM_017352452.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5673bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product microtubule cross-linking factor 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017352452.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGAGAGCT CCAATGGCTC CGACACCAAA CCGCAGAGCC ATCATCCTCA GGTGGAGAAG 
    AAGAGGTTAA ACCGCGCGCC GTCCCCTGCC AGACCCGTCC TCAAGGATGT CCACTCGCGG
    ACGGCCAAAC CGATCGTGCC AAAATCGCCC AAATTCAACA AGCAGCAAGC GCCGGTGACG
    CCTCAGAGCC GCGTCCCGAG ACGCACCGTC CCGGGCGCGA AGGACAAAAC CGCATCAGCA
    CCTGCAAAAA GCAACACCAA GCCCGCCTCA GCGACAAAAA AGCCCGTTAA GGTCACTCAG
    CATGCAGCAT CCGAACCCAA ACCGGCCGGT AAGGCTGCGG AGTCCGCCGC TCCGCAGATC
    ACCAAGAGCA AGGGGAGACC TGCCCCCATA CGCGTGCCCA CCGGACGCGG CGTCTGTCAG
    ACCGACAGCA GCTCGGATTT GTCTGACTGT CCTTCCGAGC CGCTGTCCGA CGAGCAGAGG
    CTGGTCCAGG CCGCCAGCAG CGACGCGGAG TCGGGCAGCG GCTCGGGTTC GAGTGAGCAG
    GCGGCTGCAG GATCACGGAC TGCACCTGCT CCTGCTCCCG TTGCGCGCGC GGACAAACGC
    AAAGATAATA AAGACGCACA GTTTAAACCC GACGGCAAGG AGATGGAGAC GGAGATGGCC
    CAGGACGAGC TGCTAAGGGA GATCGAAGAC CTGCGATCGG AAAATGACTA CCTCAAGGAT
    GAAGTGGATG AGCTGCGAGC AGAGATGGAG GAGATCCGGG ACAGTTACCT GGAAGAGGAG
    GTGTACCAGC TGCAAGATCT GCGCCGGGAA CTGGACCGCT CCAACAAAAA CTGCCGGATC
    CTCCAGTACC GCCTGCGCAA GGCTGAACAG AAGAGCCTGC GTGTAGCACA GACCGGACAT
    GTGGACGGAG AGCTTCTGCG CGGCCTGGAG CAGGACCTCA AGGTGGCCAA AGATGTATCG
    GTGCGTCTGC ACAATGAACT AGAAACGGTG GAAGACAAGC GAACGCGGGC GGAAGATGAA
    AACGAGCTGC TCAGGCAAAA GATCATCGAA ATCGAGATCT CCAAACAGGC CCTGCATAAT
    GAGCTGGAGA GAGCCAAAGA GACTGCTCTG CGGAGGCGAG GCAGTAGAGA AGCCTTCAAA
    GATAAGAAGC CTTTATCTCA GGAGGACAGT GCAGATCTGC GCTGTCAGCT ACAGTTTGCC
    AAGGAGGAAT CGACTCTCAT GCGTAAAAAG ATGGCCAAAC TTGGCCGCGA GAAGGATGAA
    CTGGAACAAG AATTGCAGAA GTATAAATCA GTGTACGGCG ACGTGGACAG CCCTTTGCCT
    TTAGCGGAGG TGACCGGCGG AGGCCCACAC ACCACACGCG AAGCTGAGCT ACGACTACGA
    CTTAAACTAG TAGAAGAAGA AGCAAACATC CTGGGGAGGA AGATTGTGGA GCTGGAAGTT
    GAAAACCGCG GTCTCAGAGC GGAAAATGAA GACTTGCGTA GTCAATATGA AAGGGATTGC
    TTTGGCCGTG AACCCTTCTC CAGCGTCCCT ACCTCACCGT TTGGCGGCGA TGCGCTTGAG
    TCTGCGAGTG AACTGCGCAG ACATTTGCAG TTTGTGGAGG AAGAAGCTGA GCTCCTGCGG
    CGCTCCATTT CTGAAATCGA AGATCACAAC AAACAGCTAA CATCCGAGCT CAACCGCTTC
    AAATTCGGTC CCAATCAATC CGAGCGGGAA TCCGAAGGAG CTGAAGGAGG ACTCAAAATT
    GTGAATACCG CAGGTAATGG TTTTTCAAGC AGCACACTTC AGGATGAACT AAAAAACGCA
    AGACTCCAAG TCAACGAGTT AAGCGGGAAA GTAATGAAGC TTCAATATGA GAATCGGGTG
    CTAATCTCTA ATATGCAGCG CTGTGATTTG GCCGCACACT TGGGAATGCG TACTGGTAGT
    CCACGGGATA GTGATGTAGA CAGCGACGCT GGCCGAAGGG AACCAGATGA AGATGAAACA
    GCAAGGATAT TGCTGCTCCA TCCCAAACGT GAAGGTCCTG TTGGCGGAGA GAGCGATTCT
    GATGATTTAT TTGAACGTAC GGCTACTTCA GGATTCGCCA GTGGTGAAAA ACCTTCAGAT
    TCTGGCGAAC TGGGAGTAAT CGAATTAGCT CAGCGTAGAC GGGAAGACCG GGAAGCGTTT
    AATAATATCA GAAGGGAAGC TGAGAGGCTC GGGAAAACAG TAGAGCGGCT CATAACGGAT
    ACGGATAGTC TTATCCGGGA AGGTCGACTG GTTGTTTTGG GAGGAGATCT TCTTGCGGAG
    GGTGTGGAAT TTAGAGGCGA CGGAGACTCA GCTGAATCTA AACAAGACTC TCAGGTGCTG
    GACTCCATTA ATGCACGCAT GAGGACTTTC CGAACCGATC TCCAGCAATT CATGGATAAA
    GTGGAGCATC TAGGGGATGG TTTACGGGAT CGGGTTGATG ATCTTTCCCC GATGCCTAAC
    CTCACAGAAT CTAGCAGCTT CCTTTCTACG GTTACCTCCA TGTCCAGGGA TTCGCCAATC
    GGGACACTGG GAAGGGATCT AGTGACGGAC TTCCAGTTTC GCGACCCTGT CCTGCCTGAG
    CTGGGAACGC CAACCCGAGA GATACAGCTC CGTCTGGACC ACGAGCGGAG ATTGAGAGCT
    GGAGCAGAGG ACAGAGAAGG CTTTACTTCA TCTATCACTC AGGAGGAGGA GAGGAGGCGT
    GCAGAGCTCC GAAGAGGACT CGAGCTGCAA GGGCTTCAGT CTCATGAGCC AACATGGCCA
    CAGGAAAGAG GCCTGCTGCA ACAGGAAGTG AGGTTGTTCC GCCACAACAT GGTGATTGTG
    TACATGAAGC TGCGCTGGAT CCTCATGCAC TGGAGACTCG GACGCAGGAC GGACACCATG
    GAGGAGGGAG CACATGCTGA GTATGAACGA CTGGAGAATA TTCCAGAACT GGGCATTATG
    ATCGATCAGG GTGACGGAGA CACGGAGCGG GAAGACAAAG TGCATCCTCA AATAACAGGA
    AGTGACATTG CAGAGCCAGG ACCACTTCTG CTGCCATCGC CGGAGAGATT CCAGTACCAG
    AAACAGGCGT GTGACAGTCG GCGTGTTCTG CATGCGCTAC GGTCTCTGCT GGAGGAGTTC
    AGGGCGGAGC TTCGGGAGGA GGCGGGGCGG CGCTGGCAGC TGCAGCAGGC GTTAGCCAAT
    GAGAGAGCGG CATGGGAAAT ACAGCGCGCT GAAATGAGGA GTCACATCAA CACGCTGCAG
    GATGCCAGAG GGGTTTCTAC AGATGCAGAT GAAGGTGCAG TTTCTACAGA TGTTTTACAG
    CTGGAGCGGG AAGAGCAGCG CCGCCTTCTG GCCGACAGTC ACAGCACGGC ACTGGAACTG
    CGCTGGAAAC TGCAGCACGG AGAAAAGCGC TGGACTCGAG AGCGCAACGA ACTTCTGGAG
    CGATTTGAGA AAGAGCGGCA GGAATGGGAC CGCAGCTTAC GGGACATGCA CCGCAAGATG
    GAAAAGATGC AGAAAGATGC TCTTCAGTCA GATGAGGGCG CAGCACATGT CTTCTCCCCT
    CAGGAAAGCC CTTGTAAGGC TCCCAGATCT CCACGTTCCC CCTGCATTGC AGCACACACT
    CGCTCACACT CCGACTCCGA GGCCACACTG GAGGAGAAGG GAGCAGTAGG AAGACCCAGG
    CCCTGTGAGG GACCCCGGCC CCCAGAGAGC CTGTTTCTGG ATGCCCTCTC CCTGGACCCC
    CTGAGCGAAG CTGAGGTTCC CCCTCCATCA CGTCTGGAGA GTGAAAAAAG GTTCCCCCAC
    CTGAAGGAAG TAAGAGACCG AGCACTGAAT GAGATTTCTG AGATTGCGGA TCCTGCCATT
    TACCCTGAAG AAGAGAAAAG TAGTGGCAGC TTACTCAGGG CAAAGTCAGT CTGCTCCATG
    AGCGACTTCC AACGTTTAAT GGACAGCTCT CCCTTTCTAC CAGACAAAAG TAAGACTGGT
    GAGCATGGGC GGGATGATGT GACCCCTCCT CTTTCTCCAG ACGACCTCAA GTACATTGAG
    GAGTTTAATA GTAAGGGTTG GGATCTGCCC ACCAGCTCTC TAGCCACTTG CCCCATTCCT
    GGCCCAGTAA TCCCCTCTCC GGTGATGGAG GCCTGGGCGG AGAGAGCAGA ATCCCGAAGG
    GCAGATTCTA CTTCAGAAGC ATTCCAGCAA GCCTCTTGGT TCTTGACCAC CAGCGCTACC
    TTGACCACCA ACACCATGAG TAGCCCTGAG TACTGCCAAA AGGTCCAACT CAGGGCAGCT
    CAGGGCAGTG ACCAGTTTGG GGTGCATGTT CTTCACAGTC CGTCTCGAGG GGAACGATCC
    GCTCCGAGTC CTTCTGAGCA GGAATACATG TTCTCCAAAG TGACCAAAGT GAAGGCAGGG
    GAAGTAGTAG GGGGTGCAGA AGAAGTATTT GGAGGAAAAT GGCCGTGTGA GGTTGGGAGT
    CATTTGGAGG CAGGACTTAG GCCTGGTGAG CGTCCATCAA TTTGTGCTGC AGTCGGCTAT
    ACCTCATCAC TAGAGCTGGA GCTGTCACGC AATCTGAGCG ATGATATGAA GGAGAAGGCA
    TATTCCGCCC GCAATGCAAT CCGATCATCT ACTACCACTG GATCCAGCCC TCCGGAAAGG
    CAGCTACGGG ACATGGCATG CCAGACGAAT GGTCTGACCT CACGAGGAAC ACAGACCACT
    CAGACTATCA GTGTGGGACT GCAAACTGAG GCTCTTCGCA CCCTCACGAG CAGCCCGCAT
    CGCTGTCTTA CCCCCAAAGG AGGAGGCTCT ACTCCCATCT CATCCCCTTC ACGTAGCCTA
    AGAAAGATGC AGTACTCTCC AGTCGTCCAA GCCAAGTTTG AACGGCCATG CTGTTCTCCA
    AAATACGGCT CACCCAAGCT TCAAAGGAAA CCTTCTAATG CTAGTAAAAC AGAACAGCCT
    GTCAGCCAAA CCCGTGCTCC CACCCCAACA ACTCCTGTCC AGCAGCAAAA AGGCAATAAC
    GAATCCGCTT GGGCTCGTTC TACGACCACC AGAGATAGCC CCGTTCACAC CACCATCAAC
    GACGGCCTCT CCAGCCTCTT CAACATCATC GACCACACGC CCATCGCCTA CGACCCCATG
    CAGAAGTTCA ACAAATCCCC AAGCCGCTCT CGTCCTACCG AATCAGGGCC AACCGAGCCC
    AAATCTCCCA GTGTTAGTGC TGCACAGGGG CCTTTGAGGA ACTCTCGGGG CCGGTCCCCA
    AGCCCTGTGC AGCTGATTGT AGAGACGCAG GGCGAGAAAA CTCCAGAGGT GATTAGCATC
    AGACAGGACC TGTCTGCTCC TCCGGGCTAC ACGTTAGCAG AGAACGCAGC ACGGATATTG
    AATAAGAAAC TACTGGAGCA GAGCTTCAGG GAGGAAAGGA GGCTTTCTGC TTCATCCGCA
    GCTGTCCCGA ACAGAACCGG AGACTCAGAG AAACCCGGAT GTCTTGAGGA TCTGCCACGT
    TCTCCTGTGG CTCCGCCTCT GGACTCCTGC TTTCTGCGTC CGGCTCGTCC TGCCAATCGG
    CGTCCTCCGT CCCGCTGGGC GTCTCACTCC CCCTCATCCT CACCCAAATA CAGCGAGCGC
    TCAGAGCGGT TCCTCTTCCC TTCGCCCGTC CGACCCATTC ACACCATCCC AGAGACTGAG
    GAGCCAAGCC TGGAGACCCG CCTGCAACCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207941.1
    CDS480..6152
    Translation

    Target ORF information:

    RefSeq Version XM_017352452.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio microtubule crosslinking factor 1 (mtcl1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017352452.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGAGAGCT CCAATGGCTC CGACACCAAA CCGCAGAGCC ATCATCCTCA GGTGGAGAAG 
    AAGAGGTTAA ACCGCGCGCC GTCCCCTGCC AGACCCGTCC TCAAGGATGT CCACTCGCGG
    ACGGCCAAAC CGATCGTGCC AAAATCGCCC AAATTCAACA AGCAGCAAGC GCCGGTGACG
    CCTCAGAGCC GCGTCCCGAG ACGCACCGTC CCGGGCGCGA AGGACAAAAC CGCATCAGCA
    CCTGCAAAAA GCAACACCAA GCCCGCCTCA GCGACAAAAA AGCCCGTTAA GGTCACTCAG
    CATGCAGCAT CCGAACCCAA ACCGGCCGGT AAGGCTGCGG AGTCCGCCGC TCCGCAGATC
    ACCAAGAGCA AGGGGAGACC TGCCCCCATA CGCGTGCCCA CCGGACGCGG CGTCTGTCAG
    ACCGACAGCA GCTCGGATTT GTCTGACTGT CCTTCCGAGC CGCTGTCCGA CGAGCAGAGG
    CTGGTCCAGG CCGCCAGCAG CGACGCGGAG TCGGGCAGCG GCTCGGGTTC GAGTGAGCAG
    GCGGCTGCAG GATCACGGAC TGCACCTGCT CCTGCTCCCG TTGCGCGCGC GGACAAACGC
    AAAGATAATA AAGACGCACA GTTTAAACCC GACGGCAAGG AGATGGAGAC GGAGATGGCC
    CAGGACGAGC TGCTAAGGGA GATCGAAGAC CTGCGATCGG AAAATGACTA CCTCAAGGAT
    GAAGTGGATG AGCTGCGAGC AGAGATGGAG GAGATCCGGG ACAGTTACCT GGAAGAGGAG
    GTGTACCAGC TGCAAGATCT GCGCCGGGAA CTGGACCGCT CCAACAAAAA CTGCCGGATC
    CTCCAGTACC GCCTGCGCAA GGCTGAACAG AAGAGCCTGC GTGTAGCACA GACCGGACAT
    GTGGACGGAG AGCTTCTGCG CGGCCTGGAG CAGGACCTCA AGGTGGCCAA AGATGTATCG
    GTGCGTCTGC ACAATGAACT AGAAACGGTG GAAGACAAGC GAACGCGGGC GGAAGATGAA
    AACGAGCTGC TCAGGCAAAA GATCATCGAA ATCGAGATCT CCAAACAGGC CCTGCATAAT
    GAGCTGGAGA GAGCCAAAGA GACTGCTCTG CGGAGGCGAG GCAGTAGAGA AGCCTTCAAA
    GATAAGAAGC CTTTATCTCA GGAGGACAGT GCAGATCTGC GCTGTCAGCT ACAGTTTGCC
    AAGGAGGAAT CGACTCTCAT GCGTAAAAAG ATGGCCAAAC TTGGCCGCGA GAAGGATGAA
    CTGGAACAAG AATTGCAGAA GTATAAATCA GTGTACGGCG ACGTGGACAG CCCTTTGCCT
    TTAGCGGAGG TGACCGGCGG AGGCCCACAC ACCACACGCG AAGCTGAGCT ACGACTACGA
    CTTAAACTAG TAGAAGAAGA AGCAAACATC CTGGGGAGGA AGATTGTGGA GCTGGAAGTT
    GAAAACCGCG GTCTCAGAGC GGAAAATGAA GACTTGCGTA GTCAATATGA AAGGGATTGC
    TTTGGCCGTG AACCCTTCTC CAGCGTCCCT ACCTCACCGT TTGGCGGCGA TGCGCTTGAG
    TCTGCGAGTG AACTGCGCAG ACATTTGCAG TTTGTGGAGG AAGAAGCTGA GCTCCTGCGG
    CGCTCCATTT CTGAAATCGA AGATCACAAC AAACAGCTAA CATCCGAGCT CAACCGCTTC
    AAATTCGGTC CCAATCAATC CGAGCGGGAA TCCGAAGGAG CTGAAGGAGG ACTCAAAATT
    GTGAATACCG CAGGTAATGG TTTTTCAAGC AGCACACTTC AGGATGAACT AAAAAACGCA
    AGACTCCAAG TCAACGAGTT AAGCGGGAAA GTAATGAAGC TTCAATATGA GAATCGGGTG
    CTAATCTCTA ATATGCAGCG CTGTGATTTG GCCGCACACT TGGGAATGCG TACTGGTAGT
    CCACGGGATA GTGATGTAGA CAGCGACGCT GGCCGAAGGG AACCAGATGA AGATGAAACA
    GCAAGGATAT TGCTGCTCCA TCCCAAACGT GAAGGTCCTG TTGGCGGAGA GAGCGATTCT
    GATGATTTAT TTGAACGTAC GGCTACTTCA GGATTCGCCA GTGGTGAAAA ACCTTCAGAT
    TCTGGCGAAC TGGGAGTAAT CGAATTAGCT CAGCGTAGAC GGGAAGACCG GGAAGCGTTT
    AATAATATCA GAAGGGAAGC TGAGAGGCTC GGGAAAACAG TAGAGCGGCT CATAACGGAT
    ACGGATAGTC TTATCCGGGA AGGTCGACTG GTTGTTTTGG GAGGAGATCT TCTTGCGGAG
    GGTGTGGAAT TTAGAGGCGA CGGAGACTCA GCTGAATCTA AACAAGACTC TCAGGTGCTG
    GACTCCATTA ATGCACGCAT GAGGACTTTC CGAACCGATC TCCAGCAATT CATGGATAAA
    GTGGAGCATC TAGGGGATGG TTTACGGGAT CGGGTTGATG ATCTTTCCCC GATGCCTAAC
    CTCACAGAAT CTAGCAGCTT CCTTTCTACG GTTACCTCCA TGTCCAGGGA TTCGCCAATC
    GGGACACTGG GAAGGGATCT AGTGACGGAC TTCCAGTTTC GCGACCCTGT CCTGCCTGAG
    CTGGGAACGC CAACCCGAGA GATACAGCTC CGTCTGGACC ACGAGCGGAG ATTGAGAGCT
    GGAGCAGAGG ACAGAGAAGG CTTTACTTCA TCTATCACTC AGGAGGAGGA GAGGAGGCGT
    GCAGAGCTCC GAAGAGGACT CGAGCTGCAA GGGCTTCAGT CTCATGAGCC AACATGGCCA
    CAGGAAAGAG GCCTGCTGCA ACAGGAAGTG AGGTTGTTCC GCCACAACAT GGTGATTGTG
    TACATGAAGC TGCGCTGGAT CCTCATGCAC TGGAGACTCG GACGCAGGAC GGACACCATG
    GAGGAGGGAG CACATGCTGA GTATGAACGA CTGGAGAATA TTCCAGAACT GGGCATTATG
    ATCGATCAGG GTGACGGAGA CACGGAGCGG GAAGACAAAG TGCATCCTCA AATAACAGGA
    AGTGACATTG CAGAGCCAGG ACCACTTCTG CTGCCATCGC CGGAGAGATT CCAGTACCAG
    AAACAGGCGT GTGACAGTCG GCGTGTTCTG CATGCGCTAC GGTCTCTGCT GGAGGAGTTC
    AGGGCGGAGC TTCGGGAGGA GGCGGGGCGG CGCTGGCAGC TGCAGCAGGC GTTAGCCAAT
    GAGAGAGCGG CATGGGAAAT ACAGCGCGCT GAAATGAGGA GTCACATCAA CACGCTGCAG
    GATGCCAGAG GGGTTTCTAC AGATGCAGAT GAAGGTGCAG TTTCTACAGA TGTTTTACAG
    CTGGAGCGGG AAGAGCAGCG CCGCCTTCTG GCCGACAGTC ACAGCACGGC ACTGGAACTG
    CGCTGGAAAC TGCAGCACGG AGAAAAGCGC TGGACTCGAG AGCGCAACGA ACTTCTGGAG
    CGATTTGAGA AAGAGCGGCA GGAATGGGAC CGCAGCTTAC GGGACATGCA CCGCAAGATG
    GAAAAGATGC AGAAAGATGC TCTTCAGTCA GATGAGGGCG CAGCACATGT CTTCTCCCCT
    CAGGAAAGCC CTTGTAAGGC TCCCAGATCT CCACGTTCCC CCTGCATTGC AGCACACACT
    CGCTCACACT CCGACTCCGA GGCCACACTG GAGGAGAAGG GAGCAGTAGG AAGACCCAGG
    CCCTGTGAGG GACCCCGGCC CCCAGAGAGC CTGTTTCTGG ATGCCCTCTC CCTGGACCCC
    CTGAGCGAAG CTGAGGTTCC CCCTCCATCA CGTCTGGAGA GTGAAAAAAG GTTCCCCCAC
    CTGAAGGAAG TAAGAGACCG AGCACTGAAT GAGATTTCTG AGATTGCGGA TCCTGCCATT
    TACCCTGAAG AAGAGAAAAG TAGTGGCAGC TTACTCAGGG CAAAGTCAGT CTGCTCCATG
    AGCGACTTCC AACGTTTAAT GGACAGCTCT CCCTTTCTAC CAGACAAAAG TAAGACTGGT
    GAGCATGGGC GGGATGATGT GACCCCTCCT CTTTCTCCAG ACGACCTCAA GTACATTGAG
    GAGTTTAATA GTAAGGGTTG GGATCTGCCC ACCAGCTCTC TAGCCACTTG CCCCATTCCT
    GGCCCAGTAA TCCCCTCTCC GGTGATGGAG GCCTGGGCGG AGAGAGCAGA ATCCCGAAGG
    GCAGATTCTA CTTCAGAAGC ATTCCAGCAA GCCTCTTGGT TCTTGACCAC CAGCGCTACC
    TTGACCACCA ACACCATGAG TAGCCCTGAG TACTGCCAAA AGGTCCAACT CAGGGCAGCT
    CAGGGCAGTG ACCAGTTTGG GGTGCATGTT CTTCACAGTC CGTCTCGAGG GGAACGATCC
    GCTCCGAGTC CTTCTGAGCA GGAATACATG TTCTCCAAAG TGACCAAAGT GAAGGCAGGG
    GAAGTAGTAG GGGGTGCAGA AGAAGTATTT GGAGGAAAAT GGCCGTGTGA GGTTGGGAGT
    CATTTGGAGG CAGGACTTAG GCCTGGTGAG CGTCCATCAA TTTGTGCTGC AGTCGGCTAT
    ACCTCATCAC TAGAGCTGGA GCTGTCACGC AATCTGAGCG ATGATATGAA GGAGAAGGCA
    TATTCCGCCC GCAATGCAAT CCGATCATCT ACTACCACTG GATCCAGCCC TCCGGAAAGG
    CAGCTACGGG ACATGGCATG CCAGACGAAT GGTCTGACCT CACGAGGAAC ACAGACCACT
    CAGACTATCA GTGTGGGACT GCAAACTGAG GCTCTTCGCA CCCTCACGAG CAGCCCGCAT
    CGCTGTCTTA CCCCCAAAGG AGGAGGCTCT ACTCCCATCT CATCCCCTTC ACGTAGCCTA
    AGAAAGATGC AGTACTCTCC AGTCGTCCAA GCCAAGTTTG AACGGCCATG CTGTTCTCCA
    AAATACGGCT CACCCAAGCT TCAAAGGAAA CCTTCTAATG CTAGTAAAAC AGAACAGCCT
    GTCAGCCAAA CCCGTGCTCC CACCCCAACA ACTCCTGTCC AGCAGCAAAA AGGCAATAAC
    GAATCCGCTT GGGCTCGTTC TACGACCACC AGAGATAGCC CCGTTCACAC CACCATCAAC
    GACGGCCTCT CCAGCCTCTT CAACATCATC GACCACACGC CCATCGCCTA CGACCCCATG
    CAGAAGTTCA ACAAATCCCC AAGCCGCTCT CGTCCTACCG AATCAGGGCC AACCGAGCCC
    AAATCTCCCA GTGTTAGTGC TGCACAGGGG CCTTTGAGGA ACTCTCGGGG CCGGTCCCCA
    AGCCCTGTGC AGCTGATTGT AGAGACGCAG GGCGAGAAAA CTCCAGAGGT GATTAGCATC
    AGACAGGACC TGTCTGCTCC TCCGGGCTAC ACGTTAGCAG AGAACGCAGC ACGGATATTG
    AATAAGAAAC TACTGGAGCA GAGCTTCAGG GAGGAAAGGA GGCTTTCTGC TTCATCCGCA
    GCTGTCCCGA ACAGAACCGG AGACTCAGAG AAACCCGGAT GTCTTGAGGA TCTGCCACGT
    TCTCCTGTGG CTCCGCCTCT GGACTCCTGC TTTCTGCGTC CGGCTCGTCC TGCCAATCGG
    CGTCCTCCGT CCCGCTGGGC GTCTCACTCC CCCTCATCCT CACCCAAATA CAGCGAGCGC
    TCAGAGCGGT TCCTCTTCCC TTCGCCCGTC CGACCCATTC ACACCATCCC AGAGACTGAG
    GAGCCAAGCC TGGAGACCCG CCTGCAACCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa56509
    Clone ID Related Accession (Same CDS sequence) XM_017352452.2 , XM_017352452.1
    Accession Version XM_017352452.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5673bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product microtubule cross-linking factor 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGAGAGCT CCAATGGCTC CGACACCAAA CCGCAGAGCC ATCATCCTCA GGTGGAGAAG 
    AAGAGGTTAA ACCGCGCGCC GTCCCCTGCC AGACCCGTCC TCAAGGATGT CCACTCGCGG
    ACGGCCAAAC CGATCGTGCC AAAATCGCCC AAATTCAACA AGCAGCAAGC GCCGGTGACG
    CCTCAGAGCC GCGTCCCGAG ACGCACCGTC CCGGGCGCGA AGGACAAAAC CGCATCAGCA
    CCTGCAAAAA GCAACACCAA GCCCGCCTCA GCGACAAAAA AGCCCGTTAA GGTCACTCAG
    CATGCAGCAT CCGAACCCAA ACCGGCCGGT AAGGCTGCGG AGTCCGCCGC TCCGCAGATC
    ACCAAGAGCA AGGGGAGACC TGCCCCCATA CGCGTGCCCA CCGGACGCGG CGTCTGTCAG
    ACCGACAGCA GCTCGGATTT GTCTGACTGT CCTTCCGAGC CGCTGTCCGA CGAGCAGAGG
    CTGGTCCAGG CCGCCAGCAG CGACGCGGAG TCGGGCAGCG GCTCGGGTTC GAGTGAGCAG
    GCGGCTGCAG GATCACGGAC TGCACCTGCT CCTGCTCCCG TTGCGCGCGC GGACAAACGC
    AAAGATAATA AAGACGCACA GTTTAAACCC GACGGCAAGG AGATGGAGAC GGAGATGGCC
    CAGGACGAGC TGCTAAGGGA GATCGAAGAC CTGCGATCGG AAAATGACTA CCTCAAGGAT
    GAAGTGGATG AGCTGCGAGC AGAGATGGAG GAGATCCGGG ACAGTTACCT GGAAGAGGAG
    GTGTACCAGC TGCAAGATCT GCGCCGGGAA CTGGACCGCT CCAACAAAAA CTGCCGGATC
    CTCCAGTACC GCCTGCGCAA GGCTGAACAG AAGAGCCTGC GTGTAGCACA GACCGGACAT
    GTGGACGGAG AGCTTCTGCG CGGCCTGGAG CAGGACCTCA AGGTGGCCAA AGATGTATCG
    GTGCGTCTGC ACAATGAACT AGAAACGGTG GAAGACAAGC GAACGCGGGC GGAAGATGAA
    AACGAGCTGC TCAGGCAAAA GATCATCGAA ATCGAGATCT CCAAACAGGC CCTGCATAAT
    GAGCTGGAGA GAGCCAAAGA GACTGCTCTG CGGAGGCGAG GCAGTAGAGA AGCCTTCAAA
    GATAAGAAGC CTTTATCTCA GGAGGACAGT GCAGATCTGC GCTGTCAGCT ACAGTTTGCC
    AAGGAGGAAT CGACTCTCAT GCGTAAAAAG ATGGCCAAAC TTGGCCGCGA GAAGGATGAA
    CTGGAACAAG AATTGCAGAA GTATAAATCA GTGTACGGCG ACGTGGACAG CCCTTTGCCT
    TTAGCGGAGG TGACCGGCGG AGGCCCACAC ACCACACGCG AAGCTGAGCT ACGACTACGA
    CTTAAACTAG TAGAAGAAGA AGCAAACATC CTGGGGAGGA AGATTGTGGA GCTGGAAGTT
    GAAAACCGCG GTCTCAGAGC GGAAAATGAA GACTTGCGTA GTCAATATGA AAGGGATTGC
    TTTGGCCGTG AACCCTTCTC CAGCGTCCCT ACCTCACCGT TTGGCGGCGA TGCGCTTGAG
    TCTGCGAGTG AACTGCGCAG ACATTTGCAG TTTGTGGAGG AAGAAGCTGA GCTCCTGCGG
    CGCTCCATTT CTGAAATCGA AGATCACAAC AAACAGCTAA CATCCGAGCT CAACCGCTTC
    AAATTCGGTC CCAATCAATC CGAGCGGGAA TCCGAAGGAG CTGAAGGAGG ACTCAAAATT
    GTGAATACCG CAGGTAATGG TTTTTCAAGC AGCACACTTC AGGATGAACT AAAAAACGCA
    AGACTCCAAG TCAACGAGTT AAGCGGGAAA GTAATGAAGC TTCAATATGA GAATCGGGTG
    CTAATCTCTA ATATGCAGCG CTGTGATTTG GCCGCACACT TGGGAATGCG TACTGGTAGT
    CCACGGGATA GTGATGTAGA CAGCGACGCT GGCCGAAGGG AACCAGATGA AGATGAAACA
    GCAAGGATAT TGCTGCTCCA TCCCAAACGT GAAGGTCCTG TTGGCGGAGA GAGCGATTCT
    GATGATTTAT TTGAACGTAC GGCTACTTCA GGATTCGCCA GTGGTGAAAA ACCTTCAGAT
    TCTGGCGAAC TGGGAGTAAT CGAATTAGCT CAGCGTAGAC GGGAAGACCG GGAAGCGTTT
    AATAATATCA GAAGGGAAGC TGAGAGGCTC GGGAAAACAG TAGAGCGGCT CATAACGGAT
    ACGGATAGTC TTATCCGGGA AGGTCGACTG GTTGTTTTGG GAGGAGATCT TCTTGCGGAG
    GGTGTGGAAT TTAGAGGCGA CGGAGACTCA GCTGAATCTA AACAAGACTC TCAGGTGCTG
    GACTCCATTA ATGCACGCAT GAGGACTTTC CGAACCGATC TCCAGCAATT CATGGATAAA
    GTGGAGCATC TAGGGGATGG TTTACGGGAT CGGGTTGATG ATCTTTCCCC GATGCCTAAC
    CTCACAGAAT CTAGCAGCTT CCTTTCTACG GTTACCTCCA TGTCCAGGGA TTCGCCAATC
    GGGACACTGG GAAGGGATCT AGTGACGGAC TTCCAGTTTC GCGACCCTGT CCTGCCTGAG
    CTGGGAACGC CAACCCGAGA GATACAGCTC CGTCTGGACC ACGAGCGGAG ATTGAGAGCT
    GGAGCAGAGG ACAGAGAAGG CTTTACTTCA TCTATCACTC AGGAGGAGGA GAGGAGGCGT
    GCAGAGCTCC GAAGAGGACT CGAGCTGCAA GGGCTTCAGT CTCATGAGCC AACATGGCCA
    CAGGAAAGAG GCCTGCTGCA ACAGGAAGTG AGGTTGTTCC GCCACAACAT GGTGATTGTG
    TACATGAAGC TGCGCTGGAT CCTCATGCAC TGGAGACTCG GACGCAGGAC GGACACCATG
    GAGGAGGGAG CACATGCTGA GTATGAACGA CTGGAGAATA TTCCAGAACT GGGCATTATG
    ATCGATCAGG GTGACGGAGA CACGGAGCGG GAAGACAAAG TGCATCCTCA AATAACAGGA
    AGTGACATTG CAGAGCCAGG ACCACTTCTG CTGCCATCGC CGGAGAGATT CCAGTACCAG
    AAACAGGCGT GTGACAGTCG GCGTGTTCTG CATGCGCTAC GGTCTCTGCT GGAGGAGTTC
    AGGGCGGAGC TTCGGGAGGA GGCGGGGCGG CGCTGGCAGC TGCAGCAGGC GTTAGCCAAT
    GAGAGAGCGG CATGGGAAAT ACAGCGCGCT GAAATGAGGA GTCACATCAA CACGCTGCAG
    GATGCCAGAG GGGTTTCTAC AGATGCAGAT GAAGGTGCAG TTTCTACAGA TGTTTTACAG
    CTGGAGCGGG AAGAGCAGCG CCGCCTTCTG GCCGACAGTC ACAGCACGGC ACTGGAACTG
    CGCTGGAAAC TGCAGCACGG AGAAAAGCGC TGGACTCGAG AGCGCAACGA ACTTCTGGAG
    CGATTTGAGA AAGAGCGGCA GGAATGGGAC CGCAGCTTAC GGGACATGCA CCGCAAGATG
    GAAAAGATGC AGAAAGATGC TCTTCAGTCA GATGAGGGCG CAGCACATGT CTTCTCCCCT
    CAGGAAAGCC CTTGTAAGGC TCCCAGATCT CCACGTTCCC CCTGCATTGC AGCACACACT
    CGCTCACACT CCGACTCCGA GGCCACACTG GAGGAGAAGG GAGCAGTAGG AAGACCCAGG
    CCCTGTGAGG GACCCCGGCC CCCAGAGAGC CTGTTTCTGG ATGCCCTCTC CCTGGACCCC
    CTGAGCGAAG CTGAGGTTCC CCCTCCATCA CGTCTGGAGA GTGAAAAAAG GTTCCCCCAC
    CTGAAGGAAG TAAGAGACCG AGCACTGAAT GAGATTTCTG AGATTGCGGA TCCTGCCATT
    TACCCTGAAG AAGAGAAAAG TAGTGGCAGC TTACTCAGGG CAAAGTCAGT CTGCTCCATG
    AGCGACTTCC AACGTTTAAT GGACAGCTCT CCCTTTCTAC CAGACAAAAG TAAGACTGGT
    GAGCATGGGC GGGATGATGT GACCCCTCCT CTTTCTCCAG ACGACCTCAA GTACATTGAG
    GAGTTTAATA GTAAGGGTTG GGATCTGCCC ACCAGCTCTC TAGCCACTTG CCCCATTCCT
    GGCCCAGTAA TCCCCTCTCC GGTGATGGAG GCCTGGGCGG AGAGAGCAGA ATCCCGAAGG
    GCAGATTCTA CTTCAGAAGC ATTCCAGCAA GCCTCTTGGT TCTTGACCAC CAGCGCTACC
    TTGACCACCA ACACCATGAG TAGCCCTGAG TACTGCCAAA AGGTCCAACT CAGGGCAGCT
    CAGGGCAGTG ACCAGTTTGG GGTGCATGTT CTTCACAGTC CGTCTCGAGG GGAACGATCC
    GCTCCGAGTC CTTCTGAGCA GGAATACATG TTCTCCAAAG TGACCAAAGT GAAGGCAGGG
    GAAGTAGTAG GGGGTGCAGA AGAAGTATTT GGAGGAAAAT GGCCGTGTGA GGTTGGGAGT
    CATTTGGAGG CAGGACTTAG GCCTGGTGAG CGTCCATCAA TTTGTGCTGC AGTCGGCTAT
    ACCTCATCAC TAGAGCTGGA GCTGTCACGC AATCTGAGCG ATGATATGAA GGAGAAGGCA
    TATTCCGCCC GCAATGCAAT CCGATCATCT ACTACCACTG GATCCAGCCC TCCGGAAAGG
    CAGCTACGGG ACATGGCATG CCAGACGAAT GGTCTGACCT CACGAGGAAC ACAGACCACT
    CAGACTATCA GTGTGGGACT GCAAACTGAG GCTCTTCGCA CCCTCACGAG CAGCCCGCAT
    CGCTGTCTTA CCCCCAAAGG AGGAGGCTCT ACTCCCATCT CATCCCCTTC ACGTAGCCTA
    AGAAAGATGC AGTACTCTCC AGTCGTCCAA GCCAAGTTTG AACGGCCATG CTGTTCTCCA
    AAATACGGCT CACCCAAGCT TCAAAGGAAA CCTTCTAATG CTAGTAAAAC AGAACAGCCT
    GTCAGCCAAA CCCGTGCTCC CACCCCAACA ACTCCTGTCC AGCAGCAAAA AGGCAATAAC
    GAATCCGCTT GGGCTCGTTC TACGACCACC AGAGATAGCC CCGTTCACAC CACCATCAAC
    GACGGCCTCT CCAGCCTCTT CAACATCATC GACCACACGC CCATCGCCTA CGACCCCATG
    CAGAAGTTCA ACAAATCCCC AAGCCGCTCT CGTCCTACCG AATCAGGGCC AACCGAGCCC
    AAATCTCCCA GTGTTAGTGC TGCACAGGGG CCTTTGAGGA ACTCTCGGGG CCGGTCCCCA
    AGCCCTGTGC AGCTGATTGT AGAGACGCAG GGCGAGAAAA CTCCAGAGGT GATTAGCATC
    AGACAGGACC TGTCTGCTCC TCCGGGCTAC ACGTTAGCAG AGAACGCAGC ACGGATATTG
    AATAAGAAAC TACTGGAGCA GAGCTTCAGG GAGGAAAGGA GGCTTTCTGC TTCATCCGCA
    GCTGTCCCGA ACAGAACCGG AGACTCAGAG AAACCCGGAT GTCTTGAGGA TCTGCCACGT
    TCTCCTGTGG CTCCGCCTCT GGACTCCTGC TTTCTGCGTC CGGCTCGTCC TGCCAATCGG
    CGTCCTCCGT CCCGCTGGGC GTCTCACTCC CCCTCATCCT CACCCAAATA CAGCGAGCGC
    TCAGAGCGGT TCCTCTTCCC TTCGCCCGTC CGACCCATTC ACACCATCCC AGAGACTGAG
    GAGCCAAGCC TGGAGACCCG CCTGCAACCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207941.1
    CDS510..6182
    Translation

    Target ORF information:

    RefSeq Version XM_017352452.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio microtubule crosslinking factor 1 (mtcl1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017352452.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGAGAGCT CCAATGGCTC CGACACCAAA CCGCAGAGCC ATCATCCTCA GGTGGAGAAG 
    AAGAGGTTAA ACCGCGCGCC GTCCCCTGCC AGACCCGTCC TCAAGGATGT CCACTCGCGG
    ACGGCCAAAC CGATCGTGCC AAAATCGCCC AAATTCAACA AGCAGCAAGC GCCGGTGACG
    CCTCAGAGCC GCGTCCCGAG ACGCACCGTC CCGGGCGCGA AGGACAAAAC CGCATCAGCA
    CCTGCAAAAA GCAACACCAA GCCCGCCTCA GCGACAAAAA AGCCCGTTAA GGTCACTCAG
    CATGCAGCAT CCGAACCCAA ACCGGCCGGT AAGGCTGCGG AGTCCGCCGC TCCGCAGATC
    ACCAAGAGCA AGGGGAGACC TGCCCCCATA CGCGTGCCCA CCGGACGCGG CGTCTGTCAG
    ACCGACAGCA GCTCGGATTT GTCTGACTGT CCTTCCGAGC CGCTGTCCGA CGAGCAGAGG
    CTGGTCCAGG CCGCCAGCAG CGACGCGGAG TCGGGCAGCG GCTCGGGTTC GAGTGAGCAG
    GCGGCTGCAG GATCACGGAC TGCACCTGCT CCTGCTCCCG TTGCGCGCGC GGACAAACGC
    AAAGATAATA AAGACGCACA GTTTAAACCC GACGGCAAGG AGATGGAGAC GGAGATGGCC
    CAGGACGAGC TGCTAAGGGA GATCGAAGAC CTGCGATCGG AAAATGACTA CCTCAAGGAT
    GAAGTGGATG AGCTGCGAGC AGAGATGGAG GAGATCCGGG ACAGTTACCT GGAAGAGGAG
    GTGTACCAGC TGCAAGATCT GCGCCGGGAA CTGGACCGCT CCAACAAAAA CTGCCGGATC
    CTCCAGTACC GCCTGCGCAA GGCTGAACAG AAGAGCCTGC GTGTAGCACA GACCGGACAT
    GTGGACGGAG AGCTTCTGCG CGGCCTGGAG CAGGACCTCA AGGTGGCCAA AGATGTATCG
    GTGCGTCTGC ACAATGAACT AGAAACGGTG GAAGACAAGC GAACGCGGGC GGAAGATGAA
    AACGAGCTGC TCAGGCAAAA GATCATCGAA ATCGAGATCT CCAAACAGGC CCTGCATAAT
    GAGCTGGAGA GAGCCAAAGA GACTGCTCTG CGGAGGCGAG GCAGTAGAGA AGCCTTCAAA
    GATAAGAAGC CTTTATCTCA GGAGGACAGT GCAGATCTGC GCTGTCAGCT ACAGTTTGCC
    AAGGAGGAAT CGACTCTCAT GCGTAAAAAG ATGGCCAAAC TTGGCCGCGA GAAGGATGAA
    CTGGAACAAG AATTGCAGAA GTATAAATCA GTGTACGGCG ACGTGGACAG CCCTTTGCCT
    TTAGCGGAGG TGACCGGCGG AGGCCCACAC ACCACACGCG AAGCTGAGCT ACGACTACGA
    CTTAAACTAG TAGAAGAAGA AGCAAACATC CTGGGGAGGA AGATTGTGGA GCTGGAAGTT
    GAAAACCGCG GTCTCAGAGC GGAAAATGAA GACTTGCGTA GTCAATATGA AAGGGATTGC
    TTTGGCCGTG AACCCTTCTC CAGCGTCCCT ACCTCACCGT TTGGCGGCGA TGCGCTTGAG
    TCTGCGAGTG AACTGCGCAG ACATTTGCAG TTTGTGGAGG AAGAAGCTGA GCTCCTGCGG
    CGCTCCATTT CTGAAATCGA AGATCACAAC AAACAGCTAA CATCCGAGCT CAACCGCTTC
    AAATTCGGTC CCAATCAATC CGAGCGGGAA TCCGAAGGAG CTGAAGGAGG ACTCAAAATT
    GTGAATACCG CAGGTAATGG TTTTTCAAGC AGCACACTTC AGGATGAACT AAAAAACGCA
    AGACTCCAAG TCAACGAGTT AAGCGGGAAA GTAATGAAGC TTCAATATGA GAATCGGGTG
    CTAATCTCTA ATATGCAGCG CTGTGATTTG GCCGCACACT TGGGAATGCG TACTGGTAGT
    CCACGGGATA GTGATGTAGA CAGCGACGCT GGCCGAAGGG AACCAGATGA AGATGAAACA
    GCAAGGATAT TGCTGCTCCA TCCCAAACGT GAAGGTCCTG TTGGCGGAGA GAGCGATTCT
    GATGATTTAT TTGAACGTAC GGCTACTTCA GGATTCGCCA GTGGTGAAAA ACCTTCAGAT
    TCTGGCGAAC TGGGAGTAAT CGAATTAGCT CAGCGTAGAC GGGAAGACCG GGAAGCGTTT
    AATAATATCA GAAGGGAAGC TGAGAGGCTC GGGAAAACAG TAGAGCGGCT CATAACGGAT
    ACGGATAGTC TTATCCGGGA AGGTCGACTG GTTGTTTTGG GAGGAGATCT TCTTGCGGAG
    GGTGTGGAAT TTAGAGGCGA CGGAGACTCA GCTGAATCTA AACAAGACTC TCAGGTGCTG
    GACTCCATTA ATGCACGCAT GAGGACTTTC CGAACCGATC TCCAGCAATT CATGGATAAA
    GTGGAGCATC TAGGGGATGG TTTACGGGAT CGGGTTGATG ATCTTTCCCC GATGCCTAAC
    CTCACAGAAT CTAGCAGCTT CCTTTCTACG GTTACCTCCA TGTCCAGGGA TTCGCCAATC
    GGGACACTGG GAAGGGATCT AGTGACGGAC TTCCAGTTTC GCGACCCTGT CCTGCCTGAG
    CTGGGAACGC CAACCCGAGA GATACAGCTC CGTCTGGACC ACGAGCGGAG ATTGAGAGCT
    GGAGCAGAGG ACAGAGAAGG CTTTACTTCA TCTATCACTC AGGAGGAGGA GAGGAGGCGT
    GCAGAGCTCC GAAGAGGACT CGAGCTGCAA GGGCTTCAGT CTCATGAGCC AACATGGCCA
    CAGGAAAGAG GCCTGCTGCA ACAGGAAGTG AGGTTGTTCC GCCACAACAT GGTGATTGTG
    TACATGAAGC TGCGCTGGAT CCTCATGCAC TGGAGACTCG GACGCAGGAC GGACACCATG
    GAGGAGGGAG CACATGCTGA GTATGAACGA CTGGAGAATA TTCCAGAACT GGGCATTATG
    ATCGATCAGG GTGACGGAGA CACGGAGCGG GAAGACAAAG TGCATCCTCA AATAACAGGA
    AGTGACATTG CAGAGCCAGG ACCACTTCTG CTGCCATCGC CGGAGAGATT CCAGTACCAG
    AAACAGGCGT GTGACAGTCG GCGTGTTCTG CATGCGCTAC GGTCTCTGCT GGAGGAGTTC
    AGGGCGGAGC TTCGGGAGGA GGCGGGGCGG CGCTGGCAGC TGCAGCAGGC GTTAGCCAAT
    GAGAGAGCGG CATGGGAAAT ACAGCGCGCT GAAATGAGGA GTCACATCAA CACGCTGCAG
    GATGCCAGAG GGGTTTCTAC AGATGCAGAT GAAGGTGCAG TTTCTACAGA TGTTTTACAG
    CTGGAGCGGG AAGAGCAGCG CCGCCTTCTG GCCGACAGTC ACAGCACGGC ACTGGAACTG
    CGCTGGAAAC TGCAGCACGG AGAAAAGCGC TGGACTCGAG AGCGCAACGA ACTTCTGGAG
    CGATTTGAGA AAGAGCGGCA GGAATGGGAC CGCAGCTTAC GGGACATGCA CCGCAAGATG
    GAAAAGATGC AGAAAGATGC TCTTCAGTCA GATGAGGGCG CAGCACATGT CTTCTCCCCT
    CAGGAAAGCC CTTGTAAGGC TCCCAGATCT CCACGTTCCC CCTGCATTGC AGCACACACT
    CGCTCACACT CCGACTCCGA GGCCACACTG GAGGAGAAGG GAGCAGTAGG AAGACCCAGG
    CCCTGTGAGG GACCCCGGCC CCCAGAGAGC CTGTTTCTGG ATGCCCTCTC CCTGGACCCC
    CTGAGCGAAG CTGAGGTTCC CCCTCCATCA CGTCTGGAGA GTGAAAAAAG GTTCCCCCAC
    CTGAAGGAAG TAAGAGACCG AGCACTGAAT GAGATTTCTG AGATTGCGGA TCCTGCCATT
    TACCCTGAAG AAGAGAAAAG TAGTGGCAGC TTACTCAGGG CAAAGTCAGT CTGCTCCATG
    AGCGACTTCC AACGTTTAAT GGACAGCTCT CCCTTTCTAC CAGACAAAAG TAAGACTGGT
    GAGCATGGGC GGGATGATGT GACCCCTCCT CTTTCTCCAG ACGACCTCAA GTACATTGAG
    GAGTTTAATA GTAAGGGTTG GGATCTGCCC ACCAGCTCTC TAGCCACTTG CCCCATTCCT
    GGCCCAGTAA TCCCCTCTCC GGTGATGGAG GCCTGGGCGG AGAGAGCAGA ATCCCGAAGG
    GCAGATTCTA CTTCAGAAGC ATTCCAGCAA GCCTCTTGGT TCTTGACCAC CAGCGCTACC
    TTGACCACCA ACACCATGAG TAGCCCTGAG TACTGCCAAA AGGTCCAACT CAGGGCAGCT
    CAGGGCAGTG ACCAGTTTGG GGTGCATGTT CTTCACAGTC CGTCTCGAGG GGAACGATCC
    GCTCCGAGTC CTTCTGAGCA GGAATACATG TTCTCCAAAG TGACCAAAGT GAAGGCAGGG
    GAAGTAGTAG GGGGTGCAGA AGAAGTATTT GGAGGAAAAT GGCCGTGTGA GGTTGGGAGT
    CATTTGGAGG CAGGACTTAG GCCTGGTGAG CGTCCATCAA TTTGTGCTGC AGTCGGCTAT
    ACCTCATCAC TAGAGCTGGA GCTGTCACGC AATCTGAGCG ATGATATGAA GGAGAAGGCA
    TATTCCGCCC GCAATGCAAT CCGATCATCT ACTACCACTG GATCCAGCCC TCCGGAAAGG
    CAGCTACGGG ACATGGCATG CCAGACGAAT GGTCTGACCT CACGAGGAAC ACAGACCACT
    CAGACTATCA GTGTGGGACT GCAAACTGAG GCTCTTCGCA CCCTCACGAG CAGCCCGCAT
    CGCTGTCTTA CCCCCAAAGG AGGAGGCTCT ACTCCCATCT CATCCCCTTC ACGTAGCCTA
    AGAAAGATGC AGTACTCTCC AGTCGTCCAA GCCAAGTTTG AACGGCCATG CTGTTCTCCA
    AAATACGGCT CACCCAAGCT TCAAAGGAAA CCTTCTAATG CTAGTAAAAC AGAACAGCCT
    GTCAGCCAAA CCCGTGCTCC CACCCCAACA ACTCCTGTCC AGCAGCAAAA AGGCAATAAC
    GAATCCGCTT GGGCTCGTTC TACGACCACC AGAGATAGCC CCGTTCACAC CACCATCAAC
    GACGGCCTCT CCAGCCTCTT CAACATCATC GACCACACGC CCATCGCCTA CGACCCCATG
    CAGAAGTTCA ACAAATCCCC AAGCCGCTCT CGTCCTACCG AATCAGGGCC AACCGAGCCC
    AAATCTCCCA GTGTTAGTGC TGCACAGGGG CCTTTGAGGA ACTCTCGGGG CCGGTCCCCA
    AGCCCTGTGC AGCTGATTGT AGAGACGCAG GGCGAGAAAA CTCCAGAGGT GATTAGCATC
    AGACAGGACC TGTCTGCTCC TCCGGGCTAC ACGTTAGCAG AGAACGCAGC ACGGATATTG
    AATAAGAAAC TACTGGAGCA GAGCTTCAGG GAGGAAAGGA GGCTTTCTGC TTCATCCGCA
    GCTGTCCCGA ACAGAACCGG AGACTCAGAG AAACCCGGAT GTCTTGAGGA TCTGCCACGT
    TCTCCTGTGG CTCCGCCTCT GGACTCCTGC TTTCTGCGTC CGGCTCGTCC TGCCAATCGG
    CGTCCTCCGT CCCGCTGGGC GTCTCACTCC CCCTCATCCT CACCCAAATA CAGCGAGCGC
    TCAGAGCGGT TCCTCTTCCC TTCGCCCGTC CGACCCATTC ACACCATCCC AGAGACTGAG
    GAGCCAAGCC TGGAGACCCG CCTGCAACCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa56510
    Clone ID Related Accession (Same CDS sequence) XM_017352453.2 , XM_017352453.1
    Accession Version XM_017352453.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5583bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product microtubule cross-linking factor 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017352453.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGAGAGCT CCAATGGCTC CGACACCAAA CCGCAGAGCC ATCATCCTCA GGTGGAGAAG 
    AAGAGGTTAA ACCGCGCGCC GTCCCCTGCC AGACCCGTCC TCAAGGATGT CCACTCGCGG
    ACGGCCAAAC CGATCGTGCC AAAATCGCCC AAATTCAACA AGCAGCAAGC GCCGGTGACG
    CCTCAGAGCC GCGTCCCGAG ACGCACCGTC CCGGGCGCGA AGGACAAAAC CGCATCAGCA
    CCTGCAAAAA GCAACACCAA GCCCGCCTCA GCGACAAAAA AGCCCGTTAA GGTCACTCAG
    CATGCAGCAT CCGAACCCAA ACCGGCCGGT AAGGCTGCGG AGTCCGCCGC TCCGCAGATC
    ACCAAGAGCA AGGGGAGACC TGCCCCCATA CGCGTGCCCA CCGGACGCGG CGTCTGTCAG
    ACCGACAGCA GCTCGGATTT GTCTGACTGT CCTTCCGAGC CGCTGTCCGA CGAGCAGAGG
    CTGGTCCAGG CCGCCAGCAG CGACGCGGAG TCGGGCAGCG GCTCGGGTTC GAGTGAGCAG
    GCGGCTGCAG GATCACGGAC TGCACCTGCT CCTGCTCCCG TTGCGCGCGC GGACAAACGC
    AAAGATAATA AAGACGCACA GTTTAAACCC GACGGCAAGG AGATGGAGAC GGAGATGGCC
    CAGGACGAGC TGCTAAGGGA GATCGAAGAC CTGCGATCGG AAAATGACTA CCTCAAGGAT
    GAAGTGGATG AGCTGCGAGC AGAGATGGAG GAGATCCGGG ACAGTTACCT GGAAGAGGAG
    GTGTACCAGC TGCAAGATCT GCGCCGGGAA CTGGACCGCT CCAACAAAAA CTGCCGGATC
    CTCCAGTACC GCCTGCGCAA GGCTGAACAG AAGAGCCTGC GTGTAGCACA GACCGGACAT
    GTGGACGGAG AGCTTCTGCG CGGCCTGGAG CAGGACCTCA AGGTGGCCAA AGATGTATCG
    GTGCGTCTGC ACAATGAACT AGAAACGGTG GAAGACAAGC GAACGCGGGC GGAAGATGAA
    AACGAGCTGC TCAGGCAAAA GATCATCGAA ATCGAGATCT CCAAACAGGC CCTGCATAAT
    GAGCTGGAGA GAGCCAAAGA GACTGCTCTG CGGAGGCGAG GCAGTAGAGA AGCCTTCAAA
    GATAAGAAGC CTTTATCTCA GGAGGACAGT GCAGATCTGC GCTGTCAGCT ACAGTTTGCC
    AAGGAGGAAT CGACTCTCAT GCGTAAAAAG ATGGCCAAAC TTGGCCGCGA GAAGGATGAA
    CTGGAACAAG AATTGCAGAA GTATAAATCA GTGTACGGCG ACGTGGACAG CCCTTTGCCT
    TTAGCGGAGG TGACCGGCGG AGGCCCACAC ACCACACGCG AAGCTGAGCT ACGACTACGA
    CTTAAACTAG TAGAAGAAGA AGCAAACATC CTGGGGAGGA AGATTGTGGA GCTGGAAGTT
    GAAAACCGCG GTCTCAGAGC GGAAAATGAA GACTTGCGTA GTCAATATGA AAGGGATTGC
    TTTGGCCGTG AACCCTTCTC CAGCGTCCCT ACCTCACCGT TTGGCGGCGA TGCGCTTGAG
    TCTGCGAGTG AACTGCGCAG ACATTTGCAG TTTGTGGAGG AAGAAGCTGA GCTCCTGCGG
    CGCTCCATTT CTGAAATCGA AGATCACAAC AAACAGCTAA CATCCGAGCT CAACCGCTTC
    AAATTCGGTC CCAATCAATC CGAGCGGGAA TCCGAAGGAG CTGAAGGAGG ACTCAAAATT
    GTGAATACCG CAGGTAATGG TTTTTCAAGC AGCACACTTC AGGATGAACT AAAAAACGCA
    AGACTCCAAG TCAACGAGTT AAGCGGGAAA GTAATGAAGC TTCAATATGA GAATCGGGTG
    CTAATCTCTA ATATGCAGCG CTGTGATTTG GCCGCACACT TGGGAATGCG TACTGGTAGT
    CCACGGGATA GTGATGTAGA CAGCGACGCT GGCCGAAGGG AACCAGATGA AGATGAAACA
    GCAAGGATAT TGCTGCTCCA TCCCAAACGT GAAGGTCCTG TTGGCGGAGA GAGCGATTCT
    GATGATTTAT TTGAACGTAC GGCTACTTCA GGATTCGCCA GTGGTGAAAA ACCTTCAGAT
    TCTGGCGAAC TGGGAGTAAT CGAATTAGCT CAGCGTAGAC GGGAAGACCG GGAAGCGTTT
    AATAATATCA GAAGGGAAGC TGAGAGGCTC GGGAAAACAG TAGAGCGGCT CATAACGGAT
    ACGGATAGTC TTATCCGGGA AGGTCGACTG GTTGTTTTGG GAGGAGATCT TCTTGCGGAG
    GGTGTGGAAT TTAGAGGCGA CGGAGACTCA GCTGAATCTA AACAAGACTC TCAGGTGCTG
    GACTCCATTA ATGCACGCAT GAGGACTTTC CGAACCGATC TCCAGCAATT CATGGATAAA
    GTGGAGCATC TAGGGGATGG TTTACGGGAT CGGGTTGATG ATCTTTCCCC GATGCCTAAC
    CTCACAGAAT CTAGCAGCTT CCTTTCTACG GTTACCTCCA TGTCCAGGGA TTCGCCAATC
    GGGACACTGG GAAGGGATCT AGTGACGGAC TTCCAGTCCG GGCGCAGGGA GGAGCAGGAG
    AGCTGTGTAG ACCGGCCCAG AGGAGCCGCG GGACTCTCTA GATACACCAG ACCACACACT
    TTCAGATCCG AGTTTCGCGA CCCTGTCCTG CCTGAGCTGG GAACGCCAAC CCGAGAGATA
    CAGCTCCGTC TGGACCACGA GCGGAGATTG AGAGCTGGAG CAGAGGACAG AGAAGGCTTT
    ACTTCATCTA TCACTCAGGA GGAGGAGAGG AGGCGTGCAG AGCTCCGAAG AGGACTCGAG
    CTGCAAGGGC TTCAGTCTCA TGAGCCAACA TGGCCACAGG AAAGAGGCCT GCTGCAACAG
    GAAGTGAGGT TGTTCCGCCA CAACATGGTG ATTGTGTACA TGAAGCTGCG CTGGATCCTC
    ATGCACTGGA GACTCGGACG CAGGACGGAC ACCATGGAGG AGGGAGCACA TGCTGAGTAT
    GAACGACTGG AGAATATTCC AGAACTGGGC ATTATGATCG ATCAGGGTGA CGGAGACACG
    GAGCGGGAAG ACAAAGTGCA TCCTCAAATA ACAGGAAGTG ACATTGCAGA GCCAGGACCA
    CTTCTGCTGC CATCGCCGGA GAGATTCCAG TACCAGAAAC AGGCGTGTGA CAGTCGGCGT
    GTTCTGCATG CGCTACGGTC TCTGCTGGAG GAGTTCAGGG CGGAGCTTCG GGAGGAGGCG
    GGGCGGCGCT GGCAGCTGCA GCAGGCGTTA GCCAATGAGA GAGCGGCATG GGAAATACAG
    CGCGCTGAAA TGAGGAGTCA CATCAACACG CTGCAGGATG CCAGAGGGGT TTCTACAGAT
    GCAGATGAAG GTGCAGTTTC TACAGATGTT TTACAGCTGG AGCGGGAAGA GCAGCGCCGC
    CTTCTGGCCG ACAGTCACAG CACGGCACTG GAACTGCGCT GGAAACTGCA GCACGGAGAA
    AAGCGCTGGA CTCGAGAGCG CAACGAACTT CTGGAGCGAT TTGAGAAAGA GCGGCAGGAA
    TGGGACCGCA GCTTACGGGA CATGCACCGC AAGATGGAAA AGATGCAGAA AGATGCTCTT
    CAGTCAGATG AGGGCGCAGC ACATGTCTTC TCCCCTCAGG AAAGCCCTTG TAAGGCTCCC
    AGATCTCCAC GTTCCCCCTG CATTGCAGCA CACACTCGCT CACACTCCGA CTCCGAGGCC
    ACACTGGAGG AGAAGGGAGC AGTAGGAAGA CCCAGGCCCT GTGAGGGACC CCGGCCCCCA
    GAGAGCCTGT TTCTGGATGC CCTCTCCCTG GACCCCCTGA GCGAAGCTGA GGTTCCCCCT
    CCATCACGTC TGGAGAGTGA AAAAAGGTTC CCCCACCTGA AGGAAGTAAG AGACCGAGCA
    CTGAATGAGA TTTCTGAGAT TGCGGATCCT GCCATTTACC CTGAAGAAGA GAAAAGTAGT
    GGCAGCTTAC TCAGGGCAAA GTCAGTCTGC TCCATGAGCG ACTTCCAACG TTTAATGGAC
    AGCTCTCCCT TTCTACCAGA CAAAAGTAAG ACTGGTGAGC ATGGGCGGGA TGATGTGACC
    CCTCCTCTTT CTCCAGACGA CCTCAAGTAC ATTGAGGAGT TTAATAGTAA GGGTTGGGAT
    CTGCCCACCA GCTCTCTAGC CACTTGCCCC ATTCCTGGCC CAGTAATCCC CTCTCCGGTG
    ATGGAGGCCT GGGCGGAGAG AGCAGAATCC CGAAGGGCAG ATTCTACTTC AGAAGCATTC
    CAGCAAGCCT CTTGGTTCTT GACCACCAGC GCTACCTTGA CCACCAACAC CATGAGTAGC
    CCTGAGTACT GCCAAAAGGT CCAACTCAGG GCAGCTCAGG GCAGTGACCA GTTTGGGGTG
    CATGTTCTTC ACAGTCCGTC TCGAGGGGAA CGATCCGCTC CGAGTCCTTC TGAGCAGGAA
    TACATGTTCT CCAAAGTGAC CAAAGTGAAG GCAGGGGAAG TAGTAGGGGG TGCAGAAGAA
    GTATTTGGAG GAAAATGGCC GTGTGAGGTT GGGAGTCATT TGGAGGCAGG ACTTAGGCCT
    GGTGAGCGTC CATCAATTTG TGCTGCAGTC GGCTATACCT CATCACTAGA GCTGGAGCTG
    TCACGCAATC TGAGCGATGA TATGAAGGAG AAGGCATATT CCGCCCGCAA TGCAATCCGA
    TCATCTACTA CCACTGGATC CAGCCCTCCG GAAAGGCAGC TACGGGACAT GGCATGCCAG
    ACGAATGGTC TGACCTCACG AGGAACACAG ACCACTCAGA CTATCAGTGT GGGACTGCAA
    ACTGAGGCTC TTCGCACCCT CACGAGCAGC CCGCATCGCT GTCTTACCCC CAAAGGAGGA
    GGCTCTACTC CCATCTCATC CCCTTCACGT AGCCTAAGAA AGATGCAGTA CTCTCCAGTC
    GTCCAAGCCA AGTTTGAACG GCCATGCTGT TCTCCAAAAT ACGGCTCACC CAAGCTTCAA
    AGGAAACCTT CTAATGCTAG TAAAACAGAA CAGCCTGTCA GCCAAACCCG TGCTCCCACC
    CCAACAACTC CTGTCCAGCA GCAAAAAGGC AATAACGAAT CCGCTTGGGC TCGTTCTACG
    ACCACCAGAG ATAGCCCCGT TCACACCACC ATCAACGACG GCCTCTCCAG CCTCTTCAAC
    ATCATCGACC ACACGCCCAT CGCCTACGAC CCCATGCAGA AGTTCAACAA ATCCCCAAGC
    CGCTCTCGTC CTACCGAATC AGGGCCAACC GAGCCCAAAT CTCCCAGTGT TAGTGCTGCA
    CAGGGGCCTT TGAGGAACTC TCGGGGCCGG TCCCCAAGCC CTGTGCAGCT GATTGTAGAG
    ACGCAGGGCG AGAAAACTCC AGAGGTGATT AGCATCAGAC AGGACCTGTC TGCTCCTCCG
    GGCTACACGT TAGCAGAGAA CGCAGCACGG ATATTGAATA AGAAACTACT GGAGCAGAGC
    TTCAGGGAGG AAAGGAGGCT TTCTGCTTCA TCCGCAGCTG TCCCGAACAG AACCGGAGAC
    TCAGAGAAAC CCGGATGTCT TGAGAACCAC ACTGTCTTAC TAACTGCACC CTGGGGACTG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207942.1
    CDS481..6063
    Translation

    Target ORF information:

    RefSeq Version XM_017352453.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio microtubule crosslinking factor 1 (mtcl1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017352453.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGAGAGCT CCAATGGCTC CGACACCAAA CCGCAGAGCC ATCATCCTCA GGTGGAGAAG 
    AAGAGGTTAA ACCGCGCGCC GTCCCCTGCC AGACCCGTCC TCAAGGATGT CCACTCGCGG
    ACGGCCAAAC CGATCGTGCC AAAATCGCCC AAATTCAACA AGCAGCAAGC GCCGGTGACG
    CCTCAGAGCC GCGTCCCGAG ACGCACCGTC CCGGGCGCGA AGGACAAAAC CGCATCAGCA
    CCTGCAAAAA GCAACACCAA GCCCGCCTCA GCGACAAAAA AGCCCGTTAA GGTCACTCAG
    CATGCAGCAT CCGAACCCAA ACCGGCCGGT AAGGCTGCGG AGTCCGCCGC TCCGCAGATC
    ACCAAGAGCA AGGGGAGACC TGCCCCCATA CGCGTGCCCA CCGGACGCGG CGTCTGTCAG
    ACCGACAGCA GCTCGGATTT GTCTGACTGT CCTTCCGAGC CGCTGTCCGA CGAGCAGAGG
    CTGGTCCAGG CCGCCAGCAG CGACGCGGAG TCGGGCAGCG GCTCGGGTTC GAGTGAGCAG
    GCGGCTGCAG GATCACGGAC TGCACCTGCT CCTGCTCCCG TTGCGCGCGC GGACAAACGC
    AAAGATAATA AAGACGCACA GTTTAAACCC GACGGCAAGG AGATGGAGAC GGAGATGGCC
    CAGGACGAGC TGCTAAGGGA GATCGAAGAC CTGCGATCGG AAAATGACTA CCTCAAGGAT
    GAAGTGGATG AGCTGCGAGC AGAGATGGAG GAGATCCGGG ACAGTTACCT GGAAGAGGAG
    GTGTACCAGC TGCAAGATCT GCGCCGGGAA CTGGACCGCT CCAACAAAAA CTGCCGGATC
    CTCCAGTACC GCCTGCGCAA GGCTGAACAG AAGAGCCTGC GTGTAGCACA GACCGGACAT
    GTGGACGGAG AGCTTCTGCG CGGCCTGGAG CAGGACCTCA AGGTGGCCAA AGATGTATCG
    GTGCGTCTGC ACAATGAACT AGAAACGGTG GAAGACAAGC GAACGCGGGC GGAAGATGAA
    AACGAGCTGC TCAGGCAAAA GATCATCGAA ATCGAGATCT CCAAACAGGC CCTGCATAAT
    GAGCTGGAGA GAGCCAAAGA GACTGCTCTG CGGAGGCGAG GCAGTAGAGA AGCCTTCAAA
    GATAAGAAGC CTTTATCTCA GGAGGACAGT GCAGATCTGC GCTGTCAGCT ACAGTTTGCC
    AAGGAGGAAT CGACTCTCAT GCGTAAAAAG ATGGCCAAAC TTGGCCGCGA GAAGGATGAA
    CTGGAACAAG AATTGCAGAA GTATAAATCA GTGTACGGCG ACGTGGACAG CCCTTTGCCT
    TTAGCGGAGG TGACCGGCGG AGGCCCACAC ACCACACGCG AAGCTGAGCT ACGACTACGA
    CTTAAACTAG TAGAAGAAGA AGCAAACATC CTGGGGAGGA AGATTGTGGA GCTGGAAGTT
    GAAAACCGCG GTCTCAGAGC GGAAAATGAA GACTTGCGTA GTCAATATGA AAGGGATTGC
    TTTGGCCGTG AACCCTTCTC CAGCGTCCCT ACCTCACCGT TTGGCGGCGA TGCGCTTGAG
    TCTGCGAGTG AACTGCGCAG ACATTTGCAG TTTGTGGAGG AAGAAGCTGA GCTCCTGCGG
    CGCTCCATTT CTGAAATCGA AGATCACAAC AAACAGCTAA CATCCGAGCT CAACCGCTTC
    AAATTCGGTC CCAATCAATC CGAGCGGGAA TCCGAAGGAG CTGAAGGAGG ACTCAAAATT
    GTGAATACCG CAGGTAATGG TTTTTCAAGC AGCACACTTC AGGATGAACT AAAAAACGCA
    AGACTCCAAG TCAACGAGTT AAGCGGGAAA GTAATGAAGC TTCAATATGA GAATCGGGTG
    CTAATCTCTA ATATGCAGCG CTGTGATTTG GCCGCACACT TGGGAATGCG TACTGGTAGT
    CCACGGGATA GTGATGTAGA CAGCGACGCT GGCCGAAGGG AACCAGATGA AGATGAAACA
    GCAAGGATAT TGCTGCTCCA TCCCAAACGT GAAGGTCCTG TTGGCGGAGA GAGCGATTCT
    GATGATTTAT TTGAACGTAC GGCTACTTCA GGATTCGCCA GTGGTGAAAA ACCTTCAGAT
    TCTGGCGAAC TGGGAGTAAT CGAATTAGCT CAGCGTAGAC GGGAAGACCG GGAAGCGTTT
    AATAATATCA GAAGGGAAGC TGAGAGGCTC GGGAAAACAG TAGAGCGGCT CATAACGGAT
    ACGGATAGTC TTATCCGGGA AGGTCGACTG GTTGTTTTGG GAGGAGATCT TCTTGCGGAG
    GGTGTGGAAT TTAGAGGCGA CGGAGACTCA GCTGAATCTA AACAAGACTC TCAGGTGCTG
    GACTCCATTA ATGCACGCAT GAGGACTTTC CGAACCGATC TCCAGCAATT CATGGATAAA
    GTGGAGCATC TAGGGGATGG TTTACGGGAT CGGGTTGATG ATCTTTCCCC GATGCCTAAC
    CTCACAGAAT CTAGCAGCTT CCTTTCTACG GTTACCTCCA TGTCCAGGGA TTCGCCAATC
    GGGACACTGG GAAGGGATCT AGTGACGGAC TTCCAGTCCG GGCGCAGGGA GGAGCAGGAG
    AGCTGTGTAG ACCGGCCCAG AGGAGCCGCG GGACTCTCTA GATACACCAG ACCACACACT
    TTCAGATCCG AGTTTCGCGA CCCTGTCCTG CCTGAGCTGG GAACGCCAAC CCGAGAGATA
    CAGCTCCGTC TGGACCACGA GCGGAGATTG AGAGCTGGAG CAGAGGACAG AGAAGGCTTT
    ACTTCATCTA TCACTCAGGA GGAGGAGAGG AGGCGTGCAG AGCTCCGAAG AGGACTCGAG
    CTGCAAGGGC TTCAGTCTCA TGAGCCAACA TGGCCACAGG AAAGAGGCCT GCTGCAACAG
    GAAGTGAGGT TGTTCCGCCA CAACATGGTG ATTGTGTACA TGAAGCTGCG CTGGATCCTC
    ATGCACTGGA GACTCGGACG CAGGACGGAC ACCATGGAGG AGGGAGCACA TGCTGAGTAT
    GAACGACTGG AGAATATTCC AGAACTGGGC ATTATGATCG ATCAGGGTGA CGGAGACACG
    GAGCGGGAAG ACAAAGTGCA TCCTCAAATA ACAGGAAGTG ACATTGCAGA GCCAGGACCA
    CTTCTGCTGC CATCGCCGGA GAGATTCCAG TACCAGAAAC AGGCGTGTGA CAGTCGGCGT
    GTTCTGCATG CGCTACGGTC TCTGCTGGAG GAGTTCAGGG CGGAGCTTCG GGAGGAGGCG
    GGGCGGCGCT GGCAGCTGCA GCAGGCGTTA GCCAATGAGA GAGCGGCATG GGAAATACAG
    CGCGCTGAAA TGAGGAGTCA CATCAACACG CTGCAGGATG CCAGAGGGGT TTCTACAGAT
    GCAGATGAAG GTGCAGTTTC TACAGATGTT TTACAGCTGG AGCGGGAAGA GCAGCGCCGC
    CTTCTGGCCG ACAGTCACAG CACGGCACTG GAACTGCGCT GGAAACTGCA GCACGGAGAA
    AAGCGCTGGA CTCGAGAGCG CAACGAACTT CTGGAGCGAT TTGAGAAAGA GCGGCAGGAA
    TGGGACCGCA GCTTACGGGA CATGCACCGC AAGATGGAAA AGATGCAGAA AGATGCTCTT
    CAGTCAGATG AGGGCGCAGC ACATGTCTTC TCCCCTCAGG AAAGCCCTTG TAAGGCTCCC
    AGATCTCCAC GTTCCCCCTG CATTGCAGCA CACACTCGCT CACACTCCGA CTCCGAGGCC
    ACACTGGAGG AGAAGGGAGC AGTAGGAAGA CCCAGGCCCT GTGAGGGACC CCGGCCCCCA
    GAGAGCCTGT TTCTGGATGC CCTCTCCCTG GACCCCCTGA GCGAAGCTGA GGTTCCCCCT
    CCATCACGTC TGGAGAGTGA AAAAAGGTTC CCCCACCTGA AGGAAGTAAG AGACCGAGCA
    CTGAATGAGA TTTCTGAGAT TGCGGATCCT GCCATTTACC CTGAAGAAGA GAAAAGTAGT
    GGCAGCTTAC TCAGGGCAAA GTCAGTCTGC TCCATGAGCG ACTTCCAACG TTTAATGGAC
    AGCTCTCCCT TTCTACCAGA CAAAAGTAAG ACTGGTGAGC ATGGGCGGGA TGATGTGACC
    CCTCCTCTTT CTCCAGACGA CCTCAAGTAC ATTGAGGAGT TTAATAGTAA GGGTTGGGAT
    CTGCCCACCA GCTCTCTAGC CACTTGCCCC ATTCCTGGCC CAGTAATCCC CTCTCCGGTG
    ATGGAGGCCT GGGCGGAGAG AGCAGAATCC CGAAGGGCAG ATTCTACTTC AGAAGCATTC
    CAGCAAGCCT CTTGGTTCTT GACCACCAGC GCTACCTTGA CCACCAACAC CATGAGTAGC
    CCTGAGTACT GCCAAAAGGT CCAACTCAGG GCAGCTCAGG GCAGTGACCA GTTTGGGGTG
    CATGTTCTTC ACAGTCCGTC TCGAGGGGAA CGATCCGCTC CGAGTCCTTC TGAGCAGGAA
    TACATGTTCT CCAAAGTGAC CAAAGTGAAG GCAGGGGAAG TAGTAGGGGG TGCAGAAGAA
    GTATTTGGAG GAAAATGGCC GTGTGAGGTT GGGAGTCATT TGGAGGCAGG ACTTAGGCCT
    GGTGAGCGTC CATCAATTTG TGCTGCAGTC GGCTATACCT CATCACTAGA GCTGGAGCTG
    TCACGCAATC TGAGCGATGA TATGAAGGAG AAGGCATATT CCGCCCGCAA TGCAATCCGA
    TCATCTACTA CCACTGGATC CAGCCCTCCG GAAAGGCAGC TACGGGACAT GGCATGCCAG
    ACGAATGGTC TGACCTCACG AGGAACACAG ACCACTCAGA CTATCAGTGT GGGACTGCAA
    ACTGAGGCTC TTCGCACCCT CACGAGCAGC CCGCATCGCT GTCTTACCCC CAAAGGAGGA
    GGCTCTACTC CCATCTCATC CCCTTCACGT AGCCTAAGAA AGATGCAGTA CTCTCCAGTC
    GTCCAAGCCA AGTTTGAACG GCCATGCTGT TCTCCAAAAT ACGGCTCACC CAAGCTTCAA
    AGGAAACCTT CTAATGCTAG TAAAACAGAA CAGCCTGTCA GCCAAACCCG TGCTCCCACC
    CCAACAACTC CTGTCCAGCA GCAAAAAGGC AATAACGAAT CCGCTTGGGC TCGTTCTACG
    ACCACCAGAG ATAGCCCCGT TCACACCACC ATCAACGACG GCCTCTCCAG CCTCTTCAAC
    ATCATCGACC ACACGCCCAT CGCCTACGAC CCCATGCAGA AGTTCAACAA ATCCCCAAGC
    CGCTCTCGTC CTACCGAATC AGGGCCAACC GAGCCCAAAT CTCCCAGTGT TAGTGCTGCA
    CAGGGGCCTT TGAGGAACTC TCGGGGCCGG TCCCCAAGCC CTGTGCAGCT GATTGTAGAG
    ACGCAGGGCG AGAAAACTCC AGAGGTGATT AGCATCAGAC AGGACCTGTC TGCTCCTCCG
    GGCTACACGT TAGCAGAGAA CGCAGCACGG ATATTGAATA AGAAACTACT GGAGCAGAGC
    TTCAGGGAGG AAAGGAGGCT TTCTGCTTCA TCCGCAGCTG TCCCGAACAG AACCGGAGAC
    TCAGAGAAAC CCGGATGTCT TGAGAACCAC ACTGTCTTAC TAACTGCACC CTGGGGACTG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa56510
    Clone ID Related Accession (Same CDS sequence) XM_017352453.2 , XM_017352453.1
    Accession Version XM_017352453.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5583bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product microtubule cross-linking factor 1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGAGAGCT CCAATGGCTC CGACACCAAA CCGCAGAGCC ATCATCCTCA GGTGGAGAAG 
    AAGAGGTTAA ACCGCGCGCC GTCCCCTGCC AGACCCGTCC TCAAGGATGT CCACTCGCGG
    ACGGCCAAAC CGATCGTGCC AAAATCGCCC AAATTCAACA AGCAGCAAGC GCCGGTGACG
    CCTCAGAGCC GCGTCCCGAG ACGCACCGTC CCGGGCGCGA AGGACAAAAC CGCATCAGCA
    CCTGCAAAAA GCAACACCAA GCCCGCCTCA GCGACAAAAA AGCCCGTTAA GGTCACTCAG
    CATGCAGCAT CCGAACCCAA ACCGGCCGGT AAGGCTGCGG AGTCCGCCGC TCCGCAGATC
    ACCAAGAGCA AGGGGAGACC TGCCCCCATA CGCGTGCCCA CCGGACGCGG CGTCTGTCAG
    ACCGACAGCA GCTCGGATTT GTCTGACTGT CCTTCCGAGC CGCTGTCCGA CGAGCAGAGG
    CTGGTCCAGG CCGCCAGCAG CGACGCGGAG TCGGGCAGCG GCTCGGGTTC GAGTGAGCAG
    GCGGCTGCAG GATCACGGAC TGCACCTGCT CCTGCTCCCG TTGCGCGCGC GGACAAACGC
    AAAGATAATA AAGACGCACA GTTTAAACCC GACGGCAAGG AGATGGAGAC GGAGATGGCC
    CAGGACGAGC TGCTAAGGGA GATCGAAGAC CTGCGATCGG AAAATGACTA CCTCAAGGAT
    GAAGTGGATG AGCTGCGAGC AGAGATGGAG GAGATCCGGG ACAGTTACCT GGAAGAGGAG
    GTGTACCAGC TGCAAGATCT GCGCCGGGAA CTGGACCGCT CCAACAAAAA CTGCCGGATC
    CTCCAGTACC GCCTGCGCAA GGCTGAACAG AAGAGCCTGC GTGTAGCACA GACCGGACAT
    GTGGACGGAG AGCTTCTGCG CGGCCTGGAG CAGGACCTCA AGGTGGCCAA AGATGTATCG
    GTGCGTCTGC ACAATGAACT AGAAACGGTG GAAGACAAGC GAACGCGGGC GGAAGATGAA
    AACGAGCTGC TCAGGCAAAA GATCATCGAA ATCGAGATCT CCAAACAGGC CCTGCATAAT
    GAGCTGGAGA GAGCCAAAGA GACTGCTCTG CGGAGGCGAG GCAGTAGAGA AGCCTTCAAA
    GATAAGAAGC CTTTATCTCA GGAGGACAGT GCAGATCTGC GCTGTCAGCT ACAGTTTGCC
    AAGGAGGAAT CGACTCTCAT GCGTAAAAAG ATGGCCAAAC TTGGCCGCGA GAAGGATGAA
    CTGGAACAAG AATTGCAGAA GTATAAATCA GTGTACGGCG ACGTGGACAG CCCTTTGCCT
    TTAGCGGAGG TGACCGGCGG AGGCCCACAC ACCACACGCG AAGCTGAGCT ACGACTACGA
    CTTAAACTAG TAGAAGAAGA AGCAAACATC CTGGGGAGGA AGATTGTGGA GCTGGAAGTT
    GAAAACCGCG GTCTCAGAGC GGAAAATGAA GACTTGCGTA GTCAATATGA AAGGGATTGC
    TTTGGCCGTG AACCCTTCTC CAGCGTCCCT ACCTCACCGT TTGGCGGCGA TGCGCTTGAG
    TCTGCGAGTG AACTGCGCAG ACATTTGCAG TTTGTGGAGG AAGAAGCTGA GCTCCTGCGG
    CGCTCCATTT CTGAAATCGA AGATCACAAC AAACAGCTAA CATCCGAGCT CAACCGCTTC
    AAATTCGGTC CCAATCAATC CGAGCGGGAA TCCGAAGGAG CTGAAGGAGG ACTCAAAATT
    GTGAATACCG CAGGTAATGG TTTTTCAAGC AGCACACTTC AGGATGAACT AAAAAACGCA
    AGACTCCAAG TCAACGAGTT AAGCGGGAAA GTAATGAAGC TTCAATATGA GAATCGGGTG
    CTAATCTCTA ATATGCAGCG CTGTGATTTG GCCGCACACT TGGGAATGCG TACTGGTAGT
    CCACGGGATA GTGATGTAGA CAGCGACGCT GGCCGAAGGG AACCAGATGA AGATGAAACA
    GCAAGGATAT TGCTGCTCCA TCCCAAACGT GAAGGTCCTG TTGGCGGAGA GAGCGATTCT
    GATGATTTAT TTGAACGTAC GGCTACTTCA GGATTCGCCA GTGGTGAAAA ACCTTCAGAT
    TCTGGCGAAC TGGGAGTAAT CGAATTAGCT CAGCGTAGAC GGGAAGACCG GGAAGCGTTT
    AATAATATCA GAAGGGAAGC TGAGAGGCTC GGGAAAACAG TAGAGCGGCT CATAACGGAT
    ACGGATAGTC TTATCCGGGA AGGTCGACTG GTTGTTTTGG GAGGAGATCT TCTTGCGGAG
    GGTGTGGAAT TTAGAGGCGA CGGAGACTCA GCTGAATCTA AACAAGACTC TCAGGTGCTG
    GACTCCATTA ATGCACGCAT GAGGACTTTC CGAACCGATC TCCAGCAATT CATGGATAAA
    GTGGAGCATC TAGGGGATGG TTTACGGGAT CGGGTTGATG ATCTTTCCCC GATGCCTAAC
    CTCACAGAAT CTAGCAGCTT CCTTTCTACG GTTACCTCCA TGTCCAGGGA TTCGCCAATC
    GGGACACTGG GAAGGGATCT AGTGACGGAC TTCCAGTCCG GGCGCAGGGA GGAGCAGGAG
    AGCTGTGTAG ACCGGCCCAG AGGAGCCGCG GGACTCTCTA GATACACCAG ACCACACACT
    TTCAGATCCG AGTTTCGCGA CCCTGTCCTG CCTGAGCTGG GAACGCCAAC CCGAGAGATA
    CAGCTCCGTC TGGACCACGA GCGGAGATTG AGAGCTGGAG CAGAGGACAG AGAAGGCTTT
    ACTTCATCTA TCACTCAGGA GGAGGAGAGG AGGCGTGCAG AGCTCCGAAG AGGACTCGAG
    CTGCAAGGGC TTCAGTCTCA TGAGCCAACA TGGCCACAGG AAAGAGGCCT GCTGCAACAG
    GAAGTGAGGT TGTTCCGCCA CAACATGGTG ATTGTGTACA TGAAGCTGCG CTGGATCCTC
    ATGCACTGGA GACTCGGACG CAGGACGGAC ACCATGGAGG AGGGAGCACA TGCTGAGTAT
    GAACGACTGG AGAATATTCC AGAACTGGGC ATTATGATCG ATCAGGGTGA CGGAGACACG
    GAGCGGGAAG ACAAAGTGCA TCCTCAAATA ACAGGAAGTG ACATTGCAGA GCCAGGACCA
    CTTCTGCTGC CATCGCCGGA GAGATTCCAG TACCAGAAAC AGGCGTGTGA CAGTCGGCGT
    GTTCTGCATG CGCTACGGTC TCTGCTGGAG GAGTTCAGGG CGGAGCTTCG GGAGGAGGCG
    GGGCGGCGCT GGCAGCTGCA GCAGGCGTTA GCCAATGAGA GAGCGGCATG GGAAATACAG
    CGCGCTGAAA TGAGGAGTCA CATCAACACG CTGCAGGATG CCAGAGGGGT TTCTACAGAT
    GCAGATGAAG GTGCAGTTTC TACAGATGTT TTACAGCTGG AGCGGGAAGA GCAGCGCCGC
    CTTCTGGCCG ACAGTCACAG CACGGCACTG GAACTGCGCT GGAAACTGCA GCACGGAGAA
    AAGCGCTGGA CTCGAGAGCG CAACGAACTT CTGGAGCGAT TTGAGAAAGA GCGGCAGGAA
    TGGGACCGCA GCTTACGGGA CATGCACCGC AAGATGGAAA AGATGCAGAA AGATGCTCTT
    CAGTCAGATG AGGGCGCAGC ACATGTCTTC TCCCCTCAGG AAAGCCCTTG TAAGGCTCCC
    AGATCTCCAC GTTCCCCCTG CATTGCAGCA CACACTCGCT CACACTCCGA CTCCGAGGCC
    ACACTGGAGG AGAAGGGAGC AGTAGGAAGA CCCAGGCCCT GTGAGGGACC CCGGCCCCCA
    GAGAGCCTGT TTCTGGATGC CCTCTCCCTG GACCCCCTGA GCGAAGCTGA GGTTCCCCCT
    CCATCACGTC TGGAGAGTGA AAAAAGGTTC CCCCACCTGA AGGAAGTAAG AGACCGAGCA
    CTGAATGAGA TTTCTGAGAT TGCGGATCCT GCCATTTACC CTGAAGAAGA GAAAAGTAGT
    GGCAGCTTAC TCAGGGCAAA GTCAGTCTGC TCCATGAGCG ACTTCCAACG TTTAATGGAC
    AGCTCTCCCT TTCTACCAGA CAAAAGTAAG ACTGGTGAGC ATGGGCGGGA TGATGTGACC
    CCTCCTCTTT CTCCAGACGA CCTCAAGTAC ATTGAGGAGT TTAATAGTAA GGGTTGGGAT
    CTGCCCACCA GCTCTCTAGC CACTTGCCCC ATTCCTGGCC CAGTAATCCC CTCTCCGGTG
    ATGGAGGCCT GGGCGGAGAG AGCAGAATCC CGAAGGGCAG ATTCTACTTC AGAAGCATTC
    CAGCAAGCCT CTTGGTTCTT GACCACCAGC GCTACCTTGA CCACCAACAC CATGAGTAGC
    CCTGAGTACT GCCAAAAGGT CCAACTCAGG GCAGCTCAGG GCAGTGACCA GTTTGGGGTG
    CATGTTCTTC ACAGTCCGTC TCGAGGGGAA CGATCCGCTC CGAGTCCTTC TGAGCAGGAA
    TACATGTTCT CCAAAGTGAC CAAAGTGAAG GCAGGGGAAG TAGTAGGGGG TGCAGAAGAA
    GTATTTGGAG GAAAATGGCC GTGTGAGGTT GGGAGTCATT TGGAGGCAGG ACTTAGGCCT
    GGTGAGCGTC CATCAATTTG TGCTGCAGTC GGCTATACCT CATCACTAGA GCTGGAGCTG
    TCACGCAATC TGAGCGATGA TATGAAGGAG AAGGCATATT CCGCCCGCAA TGCAATCCGA
    TCATCTACTA CCACTGGATC CAGCCCTCCG GAAAGGCAGC TACGGGACAT GGCATGCCAG
    ACGAATGGTC TGACCTCACG AGGAACACAG ACCACTCAGA CTATCAGTGT GGGACTGCAA
    ACTGAGGCTC TTCGCACCCT CACGAGCAGC CCGCATCGCT GTCTTACCCC CAAAGGAGGA
    GGCTCTACTC CCATCTCATC CCCTTCACGT AGCCTAAGAA AGATGCAGTA CTCTCCAGTC
    GTCCAAGCCA AGTTTGAACG GCCATGCTGT TCTCCAAAAT ACGGCTCACC CAAGCTTCAA
    AGGAAACCTT CTAATGCTAG TAAAACAGAA CAGCCTGTCA GCCAAACCCG TGCTCCCACC
    CCAACAACTC CTGTCCAGCA GCAAAAAGGC AATAACGAAT CCGCTTGGGC TCGTTCTACG
    ACCACCAGAG ATAGCCCCGT TCACACCACC ATCAACGACG GCCTCTCCAG CCTCTTCAAC
    ATCATCGACC ACACGCCCAT CGCCTACGAC CCCATGCAGA AGTTCAACAA ATCCCCAAGC
    CGCTCTCGTC CTACCGAATC AGGGCCAACC GAGCCCAAAT CTCCCAGTGT TAGTGCTGCA
    CAGGGGCCTT TGAGGAACTC TCGGGGCCGG TCCCCAAGCC CTGTGCAGCT GATTGTAGAG
    ACGCAGGGCG AGAAAACTCC AGAGGTGATT AGCATCAGAC AGGACCTGTC TGCTCCTCCG
    GGCTACACGT TAGCAGAGAA CGCAGCACGG ATATTGAATA AGAAACTACT GGAGCAGAGC
    TTCAGGGAGG AAAGGAGGCT TTCTGCTTCA TCCGCAGCTG TCCCGAACAG AACCGGAGAC
    TCAGAGAAAC CCGGATGTCT TGAGAACCAC ACTGTCTTAC TAACTGCACC CTGGGGACTG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207942.1
    CDS510..6092
    Translation

    Target ORF information:

    RefSeq Version XM_017352453.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio microtubule crosslinking factor 1 (mtcl1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017352453.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGAGAGCT CCAATGGCTC CGACACCAAA CCGCAGAGCC ATCATCCTCA GGTGGAGAAG 
    AAGAGGTTAA ACCGCGCGCC GTCCCCTGCC AGACCCGTCC TCAAGGATGT CCACTCGCGG
    ACGGCCAAAC CGATCGTGCC AAAATCGCCC AAATTCAACA AGCAGCAAGC GCCGGTGACG
    CCTCAGAGCC GCGTCCCGAG ACGCACCGTC CCGGGCGCGA AGGACAAAAC CGCATCAGCA
    CCTGCAAAAA GCAACACCAA GCCCGCCTCA GCGACAAAAA AGCCCGTTAA GGTCACTCAG
    CATGCAGCAT CCGAACCCAA ACCGGCCGGT AAGGCTGCGG AGTCCGCCGC TCCGCAGATC
    ACCAAGAGCA AGGGGAGACC TGCCCCCATA CGCGTGCCCA CCGGACGCGG CGTCTGTCAG
    ACCGACAGCA GCTCGGATTT GTCTGACTGT CCTTCCGAGC CGCTGTCCGA CGAGCAGAGG
    CTGGTCCAGG CCGCCAGCAG CGACGCGGAG TCGGGCAGCG GCTCGGGTTC GAGTGAGCAG
    GCGGCTGCAG GATCACGGAC TGCACCTGCT CCTGCTCCCG TTGCGCGCGC GGACAAACGC
    AAAGATAATA AAGACGCACA GTTTAAACCC GACGGCAAGG AGATGGAGAC GGAGATGGCC
    CAGGACGAGC TGCTAAGGGA GATCGAAGAC CTGCGATCGG AAAATGACTA CCTCAAGGAT
    GAAGTGGATG AGCTGCGAGC AGAGATGGAG GAGATCCGGG ACAGTTACCT GGAAGAGGAG
    GTGTACCAGC TGCAAGATCT GCGCCGGGAA CTGGACCGCT CCAACAAAAA CTGCCGGATC
    CTCCAGTACC GCCTGCGCAA GGCTGAACAG AAGAGCCTGC GTGTAGCACA GACCGGACAT
    GTGGACGGAG AGCTTCTGCG CGGCCTGGAG CAGGACCTCA AGGTGGCCAA AGATGTATCG
    GTGCGTCTGC ACAATGAACT AGAAACGGTG GAAGACAAGC GAACGCGGGC GGAAGATGAA
    AACGAGCTGC TCAGGCAAAA GATCATCGAA ATCGAGATCT CCAAACAGGC CCTGCATAAT
    GAGCTGGAGA GAGCCAAAGA GACTGCTCTG CGGAGGCGAG GCAGTAGAGA AGCCTTCAAA
    GATAAGAAGC CTTTATCTCA GGAGGACAGT GCAGATCTGC GCTGTCAGCT ACAGTTTGCC
    AAGGAGGAAT CGACTCTCAT GCGTAAAAAG ATGGCCAAAC TTGGCCGCGA GAAGGATGAA
    CTGGAACAAG AATTGCAGAA GTATAAATCA GTGTACGGCG ACGTGGACAG CCCTTTGCCT
    TTAGCGGAGG TGACCGGCGG AGGCCCACAC ACCACACGCG AAGCTGAGCT ACGACTACGA
    CTTAAACTAG TAGAAGAAGA AGCAAACATC CTGGGGAGGA AGATTGTGGA GCTGGAAGTT
    GAAAACCGCG GTCTCAGAGC GGAAAATGAA GACTTGCGTA GTCAATATGA AAGGGATTGC
    TTTGGCCGTG AACCCTTCTC CAGCGTCCCT ACCTCACCGT TTGGCGGCGA TGCGCTTGAG
    TCTGCGAGTG AACTGCGCAG ACATTTGCAG TTTGTGGAGG AAGAAGCTGA GCTCCTGCGG
    CGCTCCATTT CTGAAATCGA AGATCACAAC AAACAGCTAA CATCCGAGCT CAACCGCTTC
    AAATTCGGTC CCAATCAATC CGAGCGGGAA TCCGAAGGAG CTGAAGGAGG ACTCAAAATT
    GTGAATACCG CAGGTAATGG TTTTTCAAGC AGCACACTTC AGGATGAACT AAAAAACGCA
    AGACTCCAAG TCAACGAGTT AAGCGGGAAA GTAATGAAGC TTCAATATGA GAATCGGGTG
    CTAATCTCTA ATATGCAGCG CTGTGATTTG GCCGCACACT TGGGAATGCG TACTGGTAGT
    CCACGGGATA GTGATGTAGA CAGCGACGCT GGCCGAAGGG AACCAGATGA AGATGAAACA
    GCAAGGATAT TGCTGCTCCA TCCCAAACGT GAAGGTCCTG TTGGCGGAGA GAGCGATTCT
    GATGATTTAT TTGAACGTAC GGCTACTTCA GGATTCGCCA GTGGTGAAAA ACCTTCAGAT
    TCTGGCGAAC TGGGAGTAAT CGAATTAGCT CAGCGTAGAC GGGAAGACCG GGAAGCGTTT
    AATAATATCA GAAGGGAAGC TGAGAGGCTC GGGAAAACAG TAGAGCGGCT CATAACGGAT
    ACGGATAGTC TTATCCGGGA AGGTCGACTG GTTGTTTTGG GAGGAGATCT TCTTGCGGAG
    GGTGTGGAAT TTAGAGGCGA CGGAGACTCA GCTGAATCTA AACAAGACTC TCAGGTGCTG
    GACTCCATTA ATGCACGCAT GAGGACTTTC CGAACCGATC TCCAGCAATT CATGGATAAA
    GTGGAGCATC TAGGGGATGG TTTACGGGAT CGGGTTGATG ATCTTTCCCC GATGCCTAAC
    CTCACAGAAT CTAGCAGCTT CCTTTCTACG GTTACCTCCA TGTCCAGGGA TTCGCCAATC
    GGGACACTGG GAAGGGATCT AGTGACGGAC TTCCAGTCCG GGCGCAGGGA GGAGCAGGAG
    AGCTGTGTAG ACCGGCCCAG AGGAGCCGCG GGACTCTCTA GATACACCAG ACCACACACT
    TTCAGATCCG AGTTTCGCGA CCCTGTCCTG CCTGAGCTGG GAACGCCAAC CCGAGAGATA
    CAGCTCCGTC TGGACCACGA GCGGAGATTG AGAGCTGGAG CAGAGGACAG AGAAGGCTTT
    ACTTCATCTA TCACTCAGGA GGAGGAGAGG AGGCGTGCAG AGCTCCGAAG AGGACTCGAG
    CTGCAAGGGC TTCAGTCTCA TGAGCCAACA TGGCCACAGG AAAGAGGCCT GCTGCAACAG
    GAAGTGAGGT TGTTCCGCCA CAACATGGTG ATTGTGTACA TGAAGCTGCG CTGGATCCTC
    ATGCACTGGA GACTCGGACG CAGGACGGAC ACCATGGAGG AGGGAGCACA TGCTGAGTAT
    GAACGACTGG AGAATATTCC AGAACTGGGC ATTATGATCG ATCAGGGTGA CGGAGACACG
    GAGCGGGAAG ACAAAGTGCA TCCTCAAATA ACAGGAAGTG ACATTGCAGA GCCAGGACCA
    CTTCTGCTGC CATCGCCGGA GAGATTCCAG TACCAGAAAC AGGCGTGTGA CAGTCGGCGT
    GTTCTGCATG CGCTACGGTC TCTGCTGGAG GAGTTCAGGG CGGAGCTTCG GGAGGAGGCG
    GGGCGGCGCT GGCAGCTGCA GCAGGCGTTA GCCAATGAGA GAGCGGCATG GGAAATACAG
    CGCGCTGAAA TGAGGAGTCA CATCAACACG CTGCAGGATG CCAGAGGGGT TTCTACAGAT
    GCAGATGAAG GTGCAGTTTC TACAGATGTT TTACAGCTGG AGCGGGAAGA GCAGCGCCGC
    CTTCTGGCCG ACAGTCACAG CACGGCACTG GAACTGCGCT GGAAACTGCA GCACGGAGAA
    AAGCGCTGGA CTCGAGAGCG CAACGAACTT CTGGAGCGAT TTGAGAAAGA GCGGCAGGAA
    TGGGACCGCA GCTTACGGGA CATGCACCGC AAGATGGAAA AGATGCAGAA AGATGCTCTT
    CAGTCAGATG AGGGCGCAGC ACATGTCTTC TCCCCTCAGG AAAGCCCTTG TAAGGCTCCC
    AGATCTCCAC GTTCCCCCTG CATTGCAGCA CACACTCGCT CACACTCCGA CTCCGAGGCC
    ACACTGGAGG AGAAGGGAGC AGTAGGAAGA CCCAGGCCCT GTGAGGGACC CCGGCCCCCA
    GAGAGCCTGT TTCTGGATGC CCTCTCCCTG GACCCCCTGA GCGAAGCTGA GGTTCCCCCT
    CCATCACGTC TGGAGAGTGA AAAAAGGTTC CCCCACCTGA AGGAAGTAAG AGACCGAGCA
    CTGAATGAGA TTTCTGAGAT TGCGGATCCT GCCATTTACC CTGAAGAAGA GAAAAGTAGT
    GGCAGCTTAC TCAGGGCAAA GTCAGTCTGC TCCATGAGCG ACTTCCAACG TTTAATGGAC
    AGCTCTCCCT TTCTACCAGA CAAAAGTAAG ACTGGTGAGC ATGGGCGGGA TGATGTGACC
    CCTCCTCTTT CTCCAGACGA CCTCAAGTAC ATTGAGGAGT TTAATAGTAA GGGTTGGGAT
    CTGCCCACCA GCTCTCTAGC CACTTGCCCC ATTCCTGGCC CAGTAATCCC CTCTCCGGTG
    ATGGAGGCCT GGGCGGAGAG AGCAGAATCC CGAAGGGCAG ATTCTACTTC AGAAGCATTC
    CAGCAAGCCT CTTGGTTCTT GACCACCAGC GCTACCTTGA CCACCAACAC CATGAGTAGC
    CCTGAGTACT GCCAAAAGGT CCAACTCAGG GCAGCTCAGG GCAGTGACCA GTTTGGGGTG
    CATGTTCTTC ACAGTCCGTC TCGAGGGGAA CGATCCGCTC CGAGTCCTTC TGAGCAGGAA
    TACATGTTCT CCAAAGTGAC CAAAGTGAAG GCAGGGGAAG TAGTAGGGGG TGCAGAAGAA
    GTATTTGGAG GAAAATGGCC GTGTGAGGTT GGGAGTCATT TGGAGGCAGG ACTTAGGCCT
    GGTGAGCGTC CATCAATTTG TGCTGCAGTC GGCTATACCT CATCACTAGA GCTGGAGCTG
    TCACGCAATC TGAGCGATGA TATGAAGGAG AAGGCATATT CCGCCCGCAA TGCAATCCGA
    TCATCTACTA CCACTGGATC CAGCCCTCCG GAAAGGCAGC TACGGGACAT GGCATGCCAG
    ACGAATGGTC TGACCTCACG AGGAACACAG ACCACTCAGA CTATCAGTGT GGGACTGCAA
    ACTGAGGCTC TTCGCACCCT CACGAGCAGC CCGCATCGCT GTCTTACCCC CAAAGGAGGA
    GGCTCTACTC CCATCTCATC CCCTTCACGT AGCCTAAGAA AGATGCAGTA CTCTCCAGTC
    GTCCAAGCCA AGTTTGAACG GCCATGCTGT TCTCCAAAAT ACGGCTCACC CAAGCTTCAA
    AGGAAACCTT CTAATGCTAG TAAAACAGAA CAGCCTGTCA GCCAAACCCG TGCTCCCACC
    CCAACAACTC CTGTCCAGCA GCAAAAAGGC AATAACGAAT CCGCTTGGGC TCGTTCTACG
    ACCACCAGAG ATAGCCCCGT TCACACCACC ATCAACGACG GCCTCTCCAG CCTCTTCAAC
    ATCATCGACC ACACGCCCAT CGCCTACGAC CCCATGCAGA AGTTCAACAA ATCCCCAAGC
    CGCTCTCGTC CTACCGAATC AGGGCCAACC GAGCCCAAAT CTCCCAGTGT TAGTGCTGCA
    CAGGGGCCTT TGAGGAACTC TCGGGGCCGG TCCCCAAGCC CTGTGCAGCT GATTGTAGAG
    ACGCAGGGCG AGAAAACTCC AGAGGTGATT AGCATCAGAC AGGACCTGTC TGCTCCTCCG
    GGCTACACGT TAGCAGAGAA CGCAGCACGG ATATTGAATA AGAAACTACT GGAGCAGAGC
    TTCAGGGAGG AAAGGAGGCT TTCTGCTTCA TCCGCAGCTG TCCCGAACAG AACCGGAGAC
    TCAGAGAAAC CCGGATGTCT TGAGAACCAC ACTGTCTTAC TAACTGCACC CTGGGGACTG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa56511
    Clone ID Related Accession (Same CDS sequence) XM_017352457.2 , XM_017352457.1
    Accession Version XM_017352457.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5583bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product microtubule cross-linking factor 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017352457.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGAGAGCT CCAATGGCTC CGACACCAAA CCGCAGAGCC ATCATCCTCA GGTGGAGAAG 
    AAGAGGTTAA ACCGCGCGCC GTCCCCTGCC AGACCCGTCC TCAAGGATGT CCACTCGCGG
    ACGGCCAAAC CGATCGTGCC AAAATCGCCC AAATTCAACA AGCAGCAAGC GCCGGTGACG
    CCTCAGAGCC GCGTCCCGAG ACGCACCGTC CCGGGCGCGA AGGACAAAAC CGCATCAGCA
    CCTGCAAAAA GCAACACCAA GCCCGCCTCA GCGACAAAAA AGCCCGTTAA GGTCACTCAG
    CATGCAGCAT CCGAACCCAA ACCGGCCGGT AAGGCTGCGG AGTCCGCCGC TCCGCAGATC
    ACCAAGAGCA AGGGGAGACC TGCCCCCATA CGCGTGCCCA CCGGACGCGG CGTCTGTCAG
    ACCGACAGCA GCTCGGATTT GTCTGACTGT CCTTCCGAGC CGCTGTCCGA CGAGCAGAGG
    CTGGTCCAGG CCGCCAGCAG CGACGCGGAG TCGGGCAGCG GCTCGGGTTC GAGTGAGCAG
    GCGGCTGCAG GATCACGGAC TGCACCTGCT CCTGCTCCCG TTGCGCGCGC GGACAAACGC
    AAAGATAATA AAGACGCACA GTTTAAACCC GACGGCAAGG AGATGGAGAC GGAGATGGCC
    CAGGACGAGC TGCTAAGGGA GATCGAAGAC CTGCGATCGG AAAATGACTA CCTCAAGGAT
    GAAGTGGATG AGCTGCGAGC AGAGATGGAG GAGATCCGGG ACAGTTACCT GGAAGAGGAG
    GTGTACCAGC TGCAAGATCT GCGCCGGGAA CTGGACCGCT CCAACAAAAA CTGCCGGATC
    CTCCAGTACC GCCTGCGCAA GGCTGAACAG AAGAGCCTGC GTGTAGCACA GACCGGACAT
    GTGGACGGAG AGCTTCTGCG CGGCCTGGAG CAGGACCTCA AGGTGGCCAA AGATGTATCG
    GTGCGTCTGC ACAATGAACT AGAAACGGTG GAAGACAAGC GAACGCGGGC GGAAGATGAA
    AACGAGCTGC TCAGGCAAAA GATCATCGAA ATCGAGATCT CCAAACAGGC CCTGCATAAT
    GAGCTGGAGA GAGCCAAAGA GACTGCTCTG CGGAGGCGAG GCAGTAGAGA AGCCTTCAAA
    GATAAGAAGC CTTTATCTCA GGAGGACAGT GCAGATCTGC GCTGTCAGCT ACAGTTTGCC
    AAGGAGGAAT CGACTCTCAT GCGTAAAAAG ATGGCCAAAC TTGGCCGCGA GAAGGATGAA
    CTGGAACAAG AATTGCAGAA GTATAAATCA GTGTACGGCG ACGTGGACAG CCCTTTGCCT
    TTAGCGGAGG TGACCGGCGG AGGCCCACAC ACCACACGCG AAGCTGAGCT ACGACTACGA
    CTTAAACTAG TAGAAGAAGA AGCAAACATC CTGGGGAGGA AGATTGTGGA GCTGGAAGTT
    GAAAACCGCG GTCTCAGAGC GGAAAATGAA GACTTGCGTA GTCAATATGA AAGGGATTGC
    TTTGGCCGTG AACCCTTCTC CAGCGTCCCT ACCTCACCGT TTGGCGGCGA TGCGCTTGAG
    TCTGCGAGTG AACTGCGCAG ACATTTGCAG TTTGTGGAGG AAGAAGCTGA GCTCCTGCGG
    CGCTCCATTT CTGAAATCGA AGATCACAAC AAACAGCTAA CATCCGAGCT CAACCGCTTC
    AAATTCGGTC CCAATCAATC CGAGCGGGAA TCCGAAGGAG CTGAAGGAGG ACTCAAAATT
    GTGAATACCG CAGGTAATGG TTTTTCAAGC AGCACACTTC AGGATGAACT AAAAAACGCA
    AGACTCCAAG TCAACGAGTT AAGCGGGAAA GTAATGAAGC TTCAATATGA GAATCGGGTG
    CTAATCTCTA ATATGCAGCG CTGTGATTTG GCCGCACACT TGGGAATGCG TACTGGTAGT
    CCACGGGATA GTGATGTAGA CAGCGACGCT GGCCGAAGGG AACCAGATGA AGATGAAACA
    GCAAGGATAT TGCTGCTCCA TCCCAAACGT GAAGGTCCTG TTGGCGGAGA GAGCGATTCT
    GATGATTTAT TTGAACGTAC GGCTACTTCA GGATTCGCCA GTGGTGAAAA ACCTTCAGAT
    TCTGGCGAAC TGGGAGTAAT CGAATTAGCT CAGCGTAGAC GGGAAGACCG GGAAGCGTTT
    AATAATATCA GAAGGGAAGC TGAGAGGCTC GGGAAAACAG TAGAGCGGCT CATAACGGAT
    ACGGATAGTC TTATCCGGGA AGGTCGACTG GTTGTTTTGG GAGGAGATCT TCTTGCGGAG
    GGTGTGGAAT TTAGAGGCGA CGGAGACTCA GCTGAATCTA AACAAGACTC TCAGGTGCTG
    GACTCCATTA ATGCACGCAT GAGGACTTTC CGAACCGATC TCCAGCAATT CATGGATAAA
    GTGGAGCATC TAGGGGATGG TTTACGGGAT CGGGTTGATG ATCTTTCCCC GATGCCTAAC
    CTCACAGAAT CTAGCAGCTT CCTTTCTACG GTTACCTCCA TGTCCAGGGA TTCGCCAATC
    GGGACACTGG GAAGGGATCT AGTGACGGAC TTCCAGTCCG GGCGCAGGGA GGAGCAGGAG
    AGCTGTGTAG ACCGGCCCAG AGGAGCCGCG GGACTCTCTA GATACACCAG ACCACACACT
    TTCAGATCCG AGTTTCGCGA CCCTGTCCTG CCTGAGCTGG GAACGCCAAC CCGAGAGATA
    CAGCTCCGTC TGGACCACGA GCGGAGATTG AGAGCTGGAG CAGAGGACAG AGAAGGCTTT
    ACTTCATCTA TCACTCAGGA GGAGGAGAGG AGGCGTGCAG AGCTCCGAAG AGGACTCGAG
    CTGCAAGGGC TTCAGTCTCA TGAGCCAACA TGGCCACAGG AAAGAGGCCT GCTGCAACAG
    GAAGTGAGGT TGTTCCGCCA CAACATGGTG ATTGTGTACA TGAAGCTGCG CTGGATCCTC
    ATGCACTGGA GACTCGGACG CAGGACGGAC ACCATGGAGG AGGGAGCACA TGCTGAGTAT
    GAACGACTGG AGAATATTCC AGAACTGGGC ATTATGATCG ATCAGGGTGA CGGAGACACG
    GAGCGGGAAG ACAAAGTGCA TCCTCAAATA ACAGGAAGTG ACATTGCAGA GCCAGGACCA
    CTTCTGCTGC CATCGCCGGA GAGATTCCAG TACCAGAAAC AGGCGTGTGA CAGTCGGCGT
    GTTCTGCATG CGCTACGGTC TCTGCTGGAG GAGTTCAGGG CGGAGCTTCG GGAGGAGGCG
    GGGCGGCGCT GGCAGCTGCA GCAGGCGTTA GCCAATGAGA GAGCGGCATG GGAAATACAG
    CGCGCTGAAA TGAGGAGTCA CATCAACACG CTGCAGGATG CCAGAGGGGT TTCTACAGAT
    GCAGATGAAG GTGCAGTTTC TACAGATGTT TTACAGCTGG AGCGGGAAGA GCAGCGCCGC
    CTTCTGGCCG ACAGTCACAG CACGGCACTG GAACTGCGCT GGAAACTGCA GCACGGAGAA
    AAGCGCTGGA CTCGAGAGCG CAACGAACTT CTGGAGCGAT TTGAGAAAGA GCGGCAGGAA
    TGGGACCGCA GCTTACGGGA CATGCACCGC AAGATGGAAA AGATGCAGAA AGATGCTCTT
    CAGTCAGATG AGGGCGCAGC ACATGTCTTC TCCCCTCAGG AAAGCCCTTG TAAGGCTCCC
    AGATCTCCAC GTTCCCCCTG CATTGCAGCA CACACTCGCT CACACTCCGA CTCCGAGGCC
    ACACTGGAGG AGAAGGGAGC AGTAGGAAGA CCCAGGCCCT GTGAGGGACC CCGGCCCCCA
    GAGAGCCTGT TTCTGGATGC CCTCTCCCTG GACCCCCTGA GCGAAGCTGA GGTTCCCCCT
    CCATCACGTC TGGAGAGTGA AAAAAGGTTC CCCCACCTGA AGGAAGTAAG AGACCGAGCA
    CTGAATGAGA TTTCTGAGAT TGCGGATCCT GCCATTTACC CTGAAGAAGA GAAAAGTAGT
    GGCAGCTTAC TCAGGGCAAA GTCAGTCTGC TCCATGAGCG ACTTCCAACG TTTAATGGAC
    AGCTCTCCCT TTCTACCAGA CAAAAGTAAG ACTGGTGAGC ATGGGCGGGA TGATGTGACC
    CCTCCTCTTT CTCCAGACGA CCTCAAGTAC ATTGAGGAGT TTAATAGTAA GGGTTGGGAT
    CTGCCCACCA GCTCTCTAGC CACTTGCCCC ATTCCTGGCC CAGTAATCCC CTCTCCGGTG
    ATGGAGGCCT GGGCGGAGAG AGCAGAATCC CGAAGGGCAG ATTCTACTTC AGAAGCATTC
    CAGCAAGCCT CTTGGTTCTT GACCACCAGC GCTACCTTGA CCACCAACAC CATGAGTAGC
    CCTGAGTACT GCCAAAAGGT CCAACTCAGG GCAGCTCAGG GCAGTGACCA GTTTGGGGTG
    CATGTTCTTC ACAGTCCGTC TCGAGGGGAA CGATCCGCTC CGAGTCCTTC TGAGCAGGAA
    TACATGTTCT CCAAAGTGAC CAAAGTGAAG GCAGGGGAAG TAGTAGGGGG TGCAGAAGAA
    GTATTTGGAG GAAAATGGCC GTGTGAGGTT GGGAGTCATT TGGAGGCAGG ACTTAGGCCT
    GGTGAGCGTC CATCAATTTG TGCTGCAGTC GGCTATACCT CATCACTAGA GCTGGAGCTG
    TCACGCAATC TGAGCGATGA TATGAAGGAG AAGGCATATT CCGCCCGCAA TGCAATCCGA
    TCATCTACTA CCACTGGATC CAGCCCTCCG GAAAGGCAGC TACGGGACAT GGCATGCCAG
    ACGAATGGTC TGACCTCACG AGGAACACAG ACCACTCAGA CTATCAGTGT GGGACTGCAA
    ACTGAGGCTC TTCGCACCCT CACGAGCAGC CCGCATCGCT GTCTTACCCC CAAAGGAGGA
    GGCTCTACTC CCATCTCATC CCCTTCACGT AGCCTAAGAA AGATGCAGTA CTCTCCAGTC
    GTCCAAGCCA AGTTTGAACG GCCATGCTGT TCTCCAAAAT ACGGCTCACC CAAGCTTCAA
    AGGAAACCTT CTAATGCTAG TAAAACAGAA CAGCCTGTCA GCCAAACCCG TGCTCCCACC
    CCAACAACTC CTGTCCAGCA GCAAAAAGGC AATAACGAAT CCGCTTGGGC TCGTTCTACG
    ACCACCAGAG ATAGCCCCGT TCACACCACC ATCAACGACG GCCTCTCCAG CCTCTTCAAC
    ATCATCGACC ACACGCCCAT CGCCTACGAC CCCATGCAGA AGTTCAACAA ATCCCCAAGC
    CGCTCTCGTC CTACCGAATC AGGGCCAACC GAGCCCAAAT CTCCCAGTGT TAGTGCTGCA
    CAGGGGCCTT TGAGGAACTC TCGGGGCCGG TCCCCAAGCC CTGTGCAGCT GATTGTAGAG
    ACGCAGGGCG AGAAAACTCC AGAGGTGATT AGCATCAGAC AGGACCTGTC TGCTCCTCCG
    GGCTACACGT TAGCAGAGAA CGCAGCACGG ATATTGAATA AGAAACTACT GGAGCAGAGC
    TTCAGGGAGG AAAGGAGGCT TTCTGCTTCA TCCGCAGCTG TCCCGAACAG AACCGGAGAC
    TCAGAGAAAC CCGGATGTCT TGAGGTTTCC CCCATGGATG TCCCGCGTGC AGATGATTGC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207946.1
    CDS481..6063
    Translation

    Target ORF information:

    RefSeq Version XM_017352457.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio microtubule crosslinking factor 1 (mtcl1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017352457.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGAGAGCT CCAATGGCTC CGACACCAAA CCGCAGAGCC ATCATCCTCA GGTGGAGAAG 
    AAGAGGTTAA ACCGCGCGCC GTCCCCTGCC AGACCCGTCC TCAAGGATGT CCACTCGCGG
    ACGGCCAAAC CGATCGTGCC AAAATCGCCC AAATTCAACA AGCAGCAAGC GCCGGTGACG
    CCTCAGAGCC GCGTCCCGAG ACGCACCGTC CCGGGCGCGA AGGACAAAAC CGCATCAGCA
    CCTGCAAAAA GCAACACCAA GCCCGCCTCA GCGACAAAAA AGCCCGTTAA GGTCACTCAG
    CATGCAGCAT CCGAACCCAA ACCGGCCGGT AAGGCTGCGG AGTCCGCCGC TCCGCAGATC
    ACCAAGAGCA AGGGGAGACC TGCCCCCATA CGCGTGCCCA CCGGACGCGG CGTCTGTCAG
    ACCGACAGCA GCTCGGATTT GTCTGACTGT CCTTCCGAGC CGCTGTCCGA CGAGCAGAGG
    CTGGTCCAGG CCGCCAGCAG CGACGCGGAG TCGGGCAGCG GCTCGGGTTC GAGTGAGCAG
    GCGGCTGCAG GATCACGGAC TGCACCTGCT CCTGCTCCCG TTGCGCGCGC GGACAAACGC
    AAAGATAATA AAGACGCACA GTTTAAACCC GACGGCAAGG AGATGGAGAC GGAGATGGCC
    CAGGACGAGC TGCTAAGGGA GATCGAAGAC CTGCGATCGG AAAATGACTA CCTCAAGGAT
    GAAGTGGATG AGCTGCGAGC AGAGATGGAG GAGATCCGGG ACAGTTACCT GGAAGAGGAG
    GTGTACCAGC TGCAAGATCT GCGCCGGGAA CTGGACCGCT CCAACAAAAA CTGCCGGATC
    CTCCAGTACC GCCTGCGCAA GGCTGAACAG AAGAGCCTGC GTGTAGCACA GACCGGACAT
    GTGGACGGAG AGCTTCTGCG CGGCCTGGAG CAGGACCTCA AGGTGGCCAA AGATGTATCG
    GTGCGTCTGC ACAATGAACT AGAAACGGTG GAAGACAAGC GAACGCGGGC GGAAGATGAA
    AACGAGCTGC TCAGGCAAAA GATCATCGAA ATCGAGATCT CCAAACAGGC CCTGCATAAT
    GAGCTGGAGA GAGCCAAAGA GACTGCTCTG CGGAGGCGAG GCAGTAGAGA AGCCTTCAAA
    GATAAGAAGC CTTTATCTCA GGAGGACAGT GCAGATCTGC GCTGTCAGCT ACAGTTTGCC
    AAGGAGGAAT CGACTCTCAT GCGTAAAAAG ATGGCCAAAC TTGGCCGCGA GAAGGATGAA
    CTGGAACAAG AATTGCAGAA GTATAAATCA GTGTACGGCG ACGTGGACAG CCCTTTGCCT
    TTAGCGGAGG TGACCGGCGG AGGCCCACAC ACCACACGCG AAGCTGAGCT ACGACTACGA
    CTTAAACTAG TAGAAGAAGA AGCAAACATC CTGGGGAGGA AGATTGTGGA GCTGGAAGTT
    GAAAACCGCG GTCTCAGAGC GGAAAATGAA GACTTGCGTA GTCAATATGA AAGGGATTGC
    TTTGGCCGTG AACCCTTCTC CAGCGTCCCT ACCTCACCGT TTGGCGGCGA TGCGCTTGAG
    TCTGCGAGTG AACTGCGCAG ACATTTGCAG TTTGTGGAGG AAGAAGCTGA GCTCCTGCGG
    CGCTCCATTT CTGAAATCGA AGATCACAAC AAACAGCTAA CATCCGAGCT CAACCGCTTC
    AAATTCGGTC CCAATCAATC CGAGCGGGAA TCCGAAGGAG CTGAAGGAGG ACTCAAAATT
    GTGAATACCG CAGGTAATGG TTTTTCAAGC AGCACACTTC AGGATGAACT AAAAAACGCA
    AGACTCCAAG TCAACGAGTT AAGCGGGAAA GTAATGAAGC TTCAATATGA GAATCGGGTG
    CTAATCTCTA ATATGCAGCG CTGTGATTTG GCCGCACACT TGGGAATGCG TACTGGTAGT
    CCACGGGATA GTGATGTAGA CAGCGACGCT GGCCGAAGGG AACCAGATGA AGATGAAACA
    GCAAGGATAT TGCTGCTCCA TCCCAAACGT GAAGGTCCTG TTGGCGGAGA GAGCGATTCT
    GATGATTTAT TTGAACGTAC GGCTACTTCA GGATTCGCCA GTGGTGAAAA ACCTTCAGAT
    TCTGGCGAAC TGGGAGTAAT CGAATTAGCT CAGCGTAGAC GGGAAGACCG GGAAGCGTTT
    AATAATATCA GAAGGGAAGC TGAGAGGCTC GGGAAAACAG TAGAGCGGCT CATAACGGAT
    ACGGATAGTC TTATCCGGGA AGGTCGACTG GTTGTTTTGG GAGGAGATCT TCTTGCGGAG
    GGTGTGGAAT TTAGAGGCGA CGGAGACTCA GCTGAATCTA AACAAGACTC TCAGGTGCTG
    GACTCCATTA ATGCACGCAT GAGGACTTTC CGAACCGATC TCCAGCAATT CATGGATAAA
    GTGGAGCATC TAGGGGATGG TTTACGGGAT CGGGTTGATG ATCTTTCCCC GATGCCTAAC
    CTCACAGAAT CTAGCAGCTT CCTTTCTACG GTTACCTCCA TGTCCAGGGA TTCGCCAATC
    GGGACACTGG GAAGGGATCT AGTGACGGAC TTCCAGTCCG GGCGCAGGGA GGAGCAGGAG
    AGCTGTGTAG ACCGGCCCAG AGGAGCCGCG GGACTCTCTA GATACACCAG ACCACACACT
    TTCAGATCCG AGTTTCGCGA CCCTGTCCTG CCTGAGCTGG GAACGCCAAC CCGAGAGATA
    CAGCTCCGTC TGGACCACGA GCGGAGATTG AGAGCTGGAG CAGAGGACAG AGAAGGCTTT
    ACTTCATCTA TCACTCAGGA GGAGGAGAGG AGGCGTGCAG AGCTCCGAAG AGGACTCGAG
    CTGCAAGGGC TTCAGTCTCA TGAGCCAACA TGGCCACAGG AAAGAGGCCT GCTGCAACAG
    GAAGTGAGGT TGTTCCGCCA CAACATGGTG ATTGTGTACA TGAAGCTGCG CTGGATCCTC
    ATGCACTGGA GACTCGGACG CAGGACGGAC ACCATGGAGG AGGGAGCACA TGCTGAGTAT
    GAACGACTGG AGAATATTCC AGAACTGGGC ATTATGATCG ATCAGGGTGA CGGAGACACG
    GAGCGGGAAG ACAAAGTGCA TCCTCAAATA ACAGGAAGTG ACATTGCAGA GCCAGGACCA
    CTTCTGCTGC CATCGCCGGA GAGATTCCAG TACCAGAAAC AGGCGTGTGA CAGTCGGCGT
    GTTCTGCATG CGCTACGGTC TCTGCTGGAG GAGTTCAGGG CGGAGCTTCG GGAGGAGGCG
    GGGCGGCGCT GGCAGCTGCA GCAGGCGTTA GCCAATGAGA GAGCGGCATG GGAAATACAG
    CGCGCTGAAA TGAGGAGTCA CATCAACACG CTGCAGGATG CCAGAGGGGT TTCTACAGAT
    GCAGATGAAG GTGCAGTTTC TACAGATGTT TTACAGCTGG AGCGGGAAGA GCAGCGCCGC
    CTTCTGGCCG ACAGTCACAG CACGGCACTG GAACTGCGCT GGAAACTGCA GCACGGAGAA
    AAGCGCTGGA CTCGAGAGCG CAACGAACTT CTGGAGCGAT TTGAGAAAGA GCGGCAGGAA
    TGGGACCGCA GCTTACGGGA CATGCACCGC AAGATGGAAA AGATGCAGAA AGATGCTCTT
    CAGTCAGATG AGGGCGCAGC ACATGTCTTC TCCCCTCAGG AAAGCCCTTG TAAGGCTCCC
    AGATCTCCAC GTTCCCCCTG CATTGCAGCA CACACTCGCT CACACTCCGA CTCCGAGGCC
    ACACTGGAGG AGAAGGGAGC AGTAGGAAGA CCCAGGCCCT GTGAGGGACC CCGGCCCCCA
    GAGAGCCTGT TTCTGGATGC CCTCTCCCTG GACCCCCTGA GCGAAGCTGA GGTTCCCCCT
    CCATCACGTC TGGAGAGTGA AAAAAGGTTC CCCCACCTGA AGGAAGTAAG AGACCGAGCA
    CTGAATGAGA TTTCTGAGAT TGCGGATCCT GCCATTTACC CTGAAGAAGA GAAAAGTAGT
    GGCAGCTTAC TCAGGGCAAA GTCAGTCTGC TCCATGAGCG ACTTCCAACG TTTAATGGAC
    AGCTCTCCCT TTCTACCAGA CAAAAGTAAG ACTGGTGAGC ATGGGCGGGA TGATGTGACC
    CCTCCTCTTT CTCCAGACGA CCTCAAGTAC ATTGAGGAGT TTAATAGTAA GGGTTGGGAT
    CTGCCCACCA GCTCTCTAGC CACTTGCCCC ATTCCTGGCC CAGTAATCCC CTCTCCGGTG
    ATGGAGGCCT GGGCGGAGAG AGCAGAATCC CGAAGGGCAG ATTCTACTTC AGAAGCATTC
    CAGCAAGCCT CTTGGTTCTT GACCACCAGC GCTACCTTGA CCACCAACAC CATGAGTAGC
    CCTGAGTACT GCCAAAAGGT CCAACTCAGG GCAGCTCAGG GCAGTGACCA GTTTGGGGTG
    CATGTTCTTC ACAGTCCGTC TCGAGGGGAA CGATCCGCTC CGAGTCCTTC TGAGCAGGAA
    TACATGTTCT CCAAAGTGAC CAAAGTGAAG GCAGGGGAAG TAGTAGGGGG TGCAGAAGAA
    GTATTTGGAG GAAAATGGCC GTGTGAGGTT GGGAGTCATT TGGAGGCAGG ACTTAGGCCT
    GGTGAGCGTC CATCAATTTG TGCTGCAGTC GGCTATACCT CATCACTAGA GCTGGAGCTG
    TCACGCAATC TGAGCGATGA TATGAAGGAG AAGGCATATT CCGCCCGCAA TGCAATCCGA
    TCATCTACTA CCACTGGATC CAGCCCTCCG GAAAGGCAGC TACGGGACAT GGCATGCCAG
    ACGAATGGTC TGACCTCACG AGGAACACAG ACCACTCAGA CTATCAGTGT GGGACTGCAA
    ACTGAGGCTC TTCGCACCCT CACGAGCAGC CCGCATCGCT GTCTTACCCC CAAAGGAGGA
    GGCTCTACTC CCATCTCATC CCCTTCACGT AGCCTAAGAA AGATGCAGTA CTCTCCAGTC
    GTCCAAGCCA AGTTTGAACG GCCATGCTGT TCTCCAAAAT ACGGCTCACC CAAGCTTCAA
    AGGAAACCTT CTAATGCTAG TAAAACAGAA CAGCCTGTCA GCCAAACCCG TGCTCCCACC
    CCAACAACTC CTGTCCAGCA GCAAAAAGGC AATAACGAAT CCGCTTGGGC TCGTTCTACG
    ACCACCAGAG ATAGCCCCGT TCACACCACC ATCAACGACG GCCTCTCCAG CCTCTTCAAC
    ATCATCGACC ACACGCCCAT CGCCTACGAC CCCATGCAGA AGTTCAACAA ATCCCCAAGC
    CGCTCTCGTC CTACCGAATC AGGGCCAACC GAGCCCAAAT CTCCCAGTGT TAGTGCTGCA
    CAGGGGCCTT TGAGGAACTC TCGGGGCCGG TCCCCAAGCC CTGTGCAGCT GATTGTAGAG
    ACGCAGGGCG AGAAAACTCC AGAGGTGATT AGCATCAGAC AGGACCTGTC TGCTCCTCCG
    GGCTACACGT TAGCAGAGAA CGCAGCACGG ATATTGAATA AGAAACTACT GGAGCAGAGC
    TTCAGGGAGG AAAGGAGGCT TTCTGCTTCA TCCGCAGCTG TCCCGAACAG AACCGGAGAC
    TCAGAGAAAC CCGGATGTCT TGAGGTTTCC CCCATGGATG TCCCGCGTGC AGATGATTGC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa56511
    Clone ID Related Accession (Same CDS sequence) XM_017352457.2 , XM_017352457.1
    Accession Version XM_017352457.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5583bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product microtubule cross-linking factor 1 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGAGAGCT CCAATGGCTC CGACACCAAA CCGCAGAGCC ATCATCCTCA GGTGGAGAAG 
    AAGAGGTTAA ACCGCGCGCC GTCCCCTGCC AGACCCGTCC TCAAGGATGT CCACTCGCGG
    ACGGCCAAAC CGATCGTGCC AAAATCGCCC AAATTCAACA AGCAGCAAGC GCCGGTGACG
    CCTCAGAGCC GCGTCCCGAG ACGCACCGTC CCGGGCGCGA AGGACAAAAC CGCATCAGCA
    CCTGCAAAAA GCAACACCAA GCCCGCCTCA GCGACAAAAA AGCCCGTTAA GGTCACTCAG
    CATGCAGCAT CCGAACCCAA ACCGGCCGGT AAGGCTGCGG AGTCCGCCGC TCCGCAGATC
    ACCAAGAGCA AGGGGAGACC TGCCCCCATA CGCGTGCCCA CCGGACGCGG CGTCTGTCAG
    ACCGACAGCA GCTCGGATTT GTCTGACTGT CCTTCCGAGC CGCTGTCCGA CGAGCAGAGG
    CTGGTCCAGG CCGCCAGCAG CGACGCGGAG TCGGGCAGCG GCTCGGGTTC GAGTGAGCAG
    GCGGCTGCAG GATCACGGAC TGCACCTGCT CCTGCTCCCG TTGCGCGCGC GGACAAACGC
    AAAGATAATA AAGACGCACA GTTTAAACCC GACGGCAAGG AGATGGAGAC GGAGATGGCC
    CAGGACGAGC TGCTAAGGGA GATCGAAGAC CTGCGATCGG AAAATGACTA CCTCAAGGAT
    GAAGTGGATG AGCTGCGAGC AGAGATGGAG GAGATCCGGG ACAGTTACCT GGAAGAGGAG
    GTGTACCAGC TGCAAGATCT GCGCCGGGAA CTGGACCGCT CCAACAAAAA CTGCCGGATC
    CTCCAGTACC GCCTGCGCAA GGCTGAACAG AAGAGCCTGC GTGTAGCACA GACCGGACAT
    GTGGACGGAG AGCTTCTGCG CGGCCTGGAG CAGGACCTCA AGGTGGCCAA AGATGTATCG
    GTGCGTCTGC ACAATGAACT AGAAACGGTG GAAGACAAGC GAACGCGGGC GGAAGATGAA
    AACGAGCTGC TCAGGCAAAA GATCATCGAA ATCGAGATCT CCAAACAGGC CCTGCATAAT
    GAGCTGGAGA GAGCCAAAGA GACTGCTCTG CGGAGGCGAG GCAGTAGAGA AGCCTTCAAA
    GATAAGAAGC CTTTATCTCA GGAGGACAGT GCAGATCTGC GCTGTCAGCT ACAGTTTGCC
    AAGGAGGAAT CGACTCTCAT GCGTAAAAAG ATGGCCAAAC TTGGCCGCGA GAAGGATGAA
    CTGGAACAAG AATTGCAGAA GTATAAATCA GTGTACGGCG ACGTGGACAG CCCTTTGCCT
    TTAGCGGAGG TGACCGGCGG AGGCCCACAC ACCACACGCG AAGCTGAGCT ACGACTACGA
    CTTAAACTAG TAGAAGAAGA AGCAAACATC CTGGGGAGGA AGATTGTGGA GCTGGAAGTT
    GAAAACCGCG GTCTCAGAGC GGAAAATGAA GACTTGCGTA GTCAATATGA AAGGGATTGC
    TTTGGCCGTG AACCCTTCTC CAGCGTCCCT ACCTCACCGT TTGGCGGCGA TGCGCTTGAG
    TCTGCGAGTG AACTGCGCAG ACATTTGCAG TTTGTGGAGG AAGAAGCTGA GCTCCTGCGG
    CGCTCCATTT CTGAAATCGA AGATCACAAC AAACAGCTAA CATCCGAGCT CAACCGCTTC
    AAATTCGGTC CCAATCAATC CGAGCGGGAA TCCGAAGGAG CTGAAGGAGG ACTCAAAATT
    GTGAATACCG CAGGTAATGG TTTTTCAAGC AGCACACTTC AGGATGAACT AAAAAACGCA
    AGACTCCAAG TCAACGAGTT AAGCGGGAAA GTAATGAAGC TTCAATATGA GAATCGGGTG
    CTAATCTCTA ATATGCAGCG CTGTGATTTG GCCGCACACT TGGGAATGCG TACTGGTAGT
    CCACGGGATA GTGATGTAGA CAGCGACGCT GGCCGAAGGG AACCAGATGA AGATGAAACA
    GCAAGGATAT TGCTGCTCCA TCCCAAACGT GAAGGTCCTG TTGGCGGAGA GAGCGATTCT
    GATGATTTAT TTGAACGTAC GGCTACTTCA GGATTCGCCA GTGGTGAAAA ACCTTCAGAT
    TCTGGCGAAC TGGGAGTAAT CGAATTAGCT CAGCGTAGAC GGGAAGACCG GGAAGCGTTT
    AATAATATCA GAAGGGAAGC TGAGAGGCTC GGGAAAACAG TAGAGCGGCT CATAACGGAT
    ACGGATAGTC TTATCCGGGA AGGTCGACTG GTTGTTTTGG GAGGAGATCT TCTTGCGGAG
    GGTGTGGAAT TTAGAGGCGA CGGAGACTCA GCTGAATCTA AACAAGACTC TCAGGTGCTG
    GACTCCATTA ATGCACGCAT GAGGACTTTC CGAACCGATC TCCAGCAATT CATGGATAAA
    GTGGAGCATC TAGGGGATGG TTTACGGGAT CGGGTTGATG ATCTTTCCCC GATGCCTAAC
    CTCACAGAAT CTAGCAGCTT CCTTTCTACG GTTACCTCCA TGTCCAGGGA TTCGCCAATC
    GGGACACTGG GAAGGGATCT AGTGACGGAC TTCCAGTCCG GGCGCAGGGA GGAGCAGGAG
    AGCTGTGTAG ACCGGCCCAG AGGAGCCGCG GGACTCTCTA GATACACCAG ACCACACACT
    TTCAGATCCG AGTTTCGCGA CCCTGTCCTG CCTGAGCTGG GAACGCCAAC CCGAGAGATA
    CAGCTCCGTC TGGACCACGA GCGGAGATTG AGAGCTGGAG CAGAGGACAG AGAAGGCTTT
    ACTTCATCTA TCACTCAGGA GGAGGAGAGG AGGCGTGCAG AGCTCCGAAG AGGACTCGAG
    CTGCAAGGGC TTCAGTCTCA TGAGCCAACA TGGCCACAGG AAAGAGGCCT GCTGCAACAG
    GAAGTGAGGT TGTTCCGCCA CAACATGGTG ATTGTGTACA TGAAGCTGCG CTGGATCCTC
    ATGCACTGGA GACTCGGACG CAGGACGGAC ACCATGGAGG AGGGAGCACA TGCTGAGTAT
    GAACGACTGG AGAATATTCC AGAACTGGGC ATTATGATCG ATCAGGGTGA CGGAGACACG
    GAGCGGGAAG ACAAAGTGCA TCCTCAAATA ACAGGAAGTG ACATTGCAGA GCCAGGACCA
    CTTCTGCTGC CATCGCCGGA GAGATTCCAG TACCAGAAAC AGGCGTGTGA CAGTCGGCGT
    GTTCTGCATG CGCTACGGTC TCTGCTGGAG GAGTTCAGGG CGGAGCTTCG GGAGGAGGCG
    GGGCGGCGCT GGCAGCTGCA GCAGGCGTTA GCCAATGAGA GAGCGGCATG GGAAATACAG
    CGCGCTGAAA TGAGGAGTCA CATCAACACG CTGCAGGATG CCAGAGGGGT TTCTACAGAT
    GCAGATGAAG GTGCAGTTTC TACAGATGTT TTACAGCTGG AGCGGGAAGA GCAGCGCCGC
    CTTCTGGCCG ACAGTCACAG CACGGCACTG GAACTGCGCT GGAAACTGCA GCACGGAGAA
    AAGCGCTGGA CTCGAGAGCG CAACGAACTT CTGGAGCGAT TTGAGAAAGA GCGGCAGGAA
    TGGGACCGCA GCTTACGGGA CATGCACCGC AAGATGGAAA AGATGCAGAA AGATGCTCTT
    CAGTCAGATG AGGGCGCAGC ACATGTCTTC TCCCCTCAGG AAAGCCCTTG TAAGGCTCCC
    AGATCTCCAC GTTCCCCCTG CATTGCAGCA CACACTCGCT CACACTCCGA CTCCGAGGCC
    ACACTGGAGG AGAAGGGAGC AGTAGGAAGA CCCAGGCCCT GTGAGGGACC CCGGCCCCCA
    GAGAGCCTGT TTCTGGATGC CCTCTCCCTG GACCCCCTGA GCGAAGCTGA GGTTCCCCCT
    CCATCACGTC TGGAGAGTGA AAAAAGGTTC CCCCACCTGA AGGAAGTAAG AGACCGAGCA
    CTGAATGAGA TTTCTGAGAT TGCGGATCCT GCCATTTACC CTGAAGAAGA GAAAAGTAGT
    GGCAGCTTAC TCAGGGCAAA GTCAGTCTGC TCCATGAGCG ACTTCCAACG TTTAATGGAC
    AGCTCTCCCT TTCTACCAGA CAAAAGTAAG ACTGGTGAGC ATGGGCGGGA TGATGTGACC
    CCTCCTCTTT CTCCAGACGA CCTCAAGTAC ATTGAGGAGT TTAATAGTAA GGGTTGGGAT
    CTGCCCACCA GCTCTCTAGC CACTTGCCCC ATTCCTGGCC CAGTAATCCC CTCTCCGGTG
    ATGGAGGCCT GGGCGGAGAG AGCAGAATCC CGAAGGGCAG ATTCTACTTC AGAAGCATTC
    CAGCAAGCCT CTTGGTTCTT GACCACCAGC GCTACCTTGA CCACCAACAC CATGAGTAGC
    CCTGAGTACT GCCAAAAGGT CCAACTCAGG GCAGCTCAGG GCAGTGACCA GTTTGGGGTG
    CATGTTCTTC ACAGTCCGTC TCGAGGGGAA CGATCCGCTC CGAGTCCTTC TGAGCAGGAA
    TACATGTTCT CCAAAGTGAC CAAAGTGAAG GCAGGGGAAG TAGTAGGGGG TGCAGAAGAA
    GTATTTGGAG GAAAATGGCC GTGTGAGGTT GGGAGTCATT TGGAGGCAGG ACTTAGGCCT
    GGTGAGCGTC CATCAATTTG TGCTGCAGTC GGCTATACCT CATCACTAGA GCTGGAGCTG
    TCACGCAATC TGAGCGATGA TATGAAGGAG AAGGCATATT CCGCCCGCAA TGCAATCCGA
    TCATCTACTA CCACTGGATC CAGCCCTCCG GAAAGGCAGC TACGGGACAT GGCATGCCAG
    ACGAATGGTC TGACCTCACG AGGAACACAG ACCACTCAGA CTATCAGTGT GGGACTGCAA
    ACTGAGGCTC TTCGCACCCT CACGAGCAGC CCGCATCGCT GTCTTACCCC CAAAGGAGGA
    GGCTCTACTC CCATCTCATC CCCTTCACGT AGCCTAAGAA AGATGCAGTA CTCTCCAGTC
    GTCCAAGCCA AGTTTGAACG GCCATGCTGT TCTCCAAAAT ACGGCTCACC CAAGCTTCAA
    AGGAAACCTT CTAATGCTAG TAAAACAGAA CAGCCTGTCA GCCAAACCCG TGCTCCCACC
    CCAACAACTC CTGTCCAGCA GCAAAAAGGC AATAACGAAT CCGCTTGGGC TCGTTCTACG
    ACCACCAGAG ATAGCCCCGT TCACACCACC ATCAACGACG GCCTCTCCAG CCTCTTCAAC
    ATCATCGACC ACACGCCCAT CGCCTACGAC CCCATGCAGA AGTTCAACAA ATCCCCAAGC
    CGCTCTCGTC CTACCGAATC AGGGCCAACC GAGCCCAAAT CTCCCAGTGT TAGTGCTGCA
    CAGGGGCCTT TGAGGAACTC TCGGGGCCGG TCCCCAAGCC CTGTGCAGCT GATTGTAGAG
    ACGCAGGGCG AGAAAACTCC AGAGGTGATT AGCATCAGAC AGGACCTGTC TGCTCCTCCG
    GGCTACACGT TAGCAGAGAA CGCAGCACGG ATATTGAATA AGAAACTACT GGAGCAGAGC
    TTCAGGGAGG AAAGGAGGCT TTCTGCTTCA TCCGCAGCTG TCCCGAACAG AACCGGAGAC
    TCAGAGAAAC CCGGATGTCT TGAGGTTTCC CCCATGGATG TCCCGCGTGC AGATGATTGC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207946.1
    CDS511..6093
    Translation

    Target ORF information:

    RefSeq Version XM_017352457.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio microtubule crosslinking factor 1 (mtcl1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017352457.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGAGAGCT CCAATGGCTC CGACACCAAA CCGCAGAGCC ATCATCCTCA GGTGGAGAAG 
    AAGAGGTTAA ACCGCGCGCC GTCCCCTGCC AGACCCGTCC TCAAGGATGT CCACTCGCGG
    ACGGCCAAAC CGATCGTGCC AAAATCGCCC AAATTCAACA AGCAGCAAGC GCCGGTGACG
    CCTCAGAGCC GCGTCCCGAG ACGCACCGTC CCGGGCGCGA AGGACAAAAC CGCATCAGCA
    CCTGCAAAAA GCAACACCAA GCCCGCCTCA GCGACAAAAA AGCCCGTTAA GGTCACTCAG
    CATGCAGCAT CCGAACCCAA ACCGGCCGGT AAGGCTGCGG AGTCCGCCGC TCCGCAGATC
    ACCAAGAGCA AGGGGAGACC TGCCCCCATA CGCGTGCCCA CCGGACGCGG CGTCTGTCAG
    ACCGACAGCA GCTCGGATTT GTCTGACTGT CCTTCCGAGC CGCTGTCCGA CGAGCAGAGG
    CTGGTCCAGG CCGCCAGCAG CGACGCGGAG TCGGGCAGCG GCTCGGGTTC GAGTGAGCAG
    GCGGCTGCAG GATCACGGAC TGCACCTGCT CCTGCTCCCG TTGCGCGCGC GGACAAACGC
    AAAGATAATA AAGACGCACA GTTTAAACCC GACGGCAAGG AGATGGAGAC GGAGATGGCC
    CAGGACGAGC TGCTAAGGGA GATCGAAGAC CTGCGATCGG AAAATGACTA CCTCAAGGAT
    GAAGTGGATG AGCTGCGAGC AGAGATGGAG GAGATCCGGG ACAGTTACCT GGAAGAGGAG
    GTGTACCAGC TGCAAGATCT GCGCCGGGAA CTGGACCGCT CCAACAAAAA CTGCCGGATC
    CTCCAGTACC GCCTGCGCAA GGCTGAACAG AAGAGCCTGC GTGTAGCACA GACCGGACAT
    GTGGACGGAG AGCTTCTGCG CGGCCTGGAG CAGGACCTCA AGGTGGCCAA AGATGTATCG
    GTGCGTCTGC ACAATGAACT AGAAACGGTG GAAGACAAGC GAACGCGGGC GGAAGATGAA
    AACGAGCTGC TCAGGCAAAA GATCATCGAA ATCGAGATCT CCAAACAGGC CCTGCATAAT
    GAGCTGGAGA GAGCCAAAGA GACTGCTCTG CGGAGGCGAG GCAGTAGAGA AGCCTTCAAA
    GATAAGAAGC CTTTATCTCA GGAGGACAGT GCAGATCTGC GCTGTCAGCT ACAGTTTGCC
    AAGGAGGAAT CGACTCTCAT GCGTAAAAAG ATGGCCAAAC TTGGCCGCGA GAAGGATGAA
    CTGGAACAAG AATTGCAGAA GTATAAATCA GTGTACGGCG ACGTGGACAG CCCTTTGCCT
    TTAGCGGAGG TGACCGGCGG AGGCCCACAC ACCACACGCG AAGCTGAGCT ACGACTACGA
    CTTAAACTAG TAGAAGAAGA AGCAAACATC CTGGGGAGGA AGATTGTGGA GCTGGAAGTT
    GAAAACCGCG GTCTCAGAGC GGAAAATGAA GACTTGCGTA GTCAATATGA AAGGGATTGC
    TTTGGCCGTG AACCCTTCTC CAGCGTCCCT ACCTCACCGT TTGGCGGCGA TGCGCTTGAG
    TCTGCGAGTG AACTGCGCAG ACATTTGCAG TTTGTGGAGG AAGAAGCTGA GCTCCTGCGG
    CGCTCCATTT CTGAAATCGA AGATCACAAC AAACAGCTAA CATCCGAGCT CAACCGCTTC
    AAATTCGGTC CCAATCAATC CGAGCGGGAA TCCGAAGGAG CTGAAGGAGG ACTCAAAATT
    GTGAATACCG CAGGTAATGG TTTTTCAAGC AGCACACTTC AGGATGAACT AAAAAACGCA
    AGACTCCAAG TCAACGAGTT AAGCGGGAAA GTAATGAAGC TTCAATATGA GAATCGGGTG
    CTAATCTCTA ATATGCAGCG CTGTGATTTG GCCGCACACT TGGGAATGCG TACTGGTAGT
    CCACGGGATA GTGATGTAGA CAGCGACGCT GGCCGAAGGG AACCAGATGA AGATGAAACA
    GCAAGGATAT TGCTGCTCCA TCCCAAACGT GAAGGTCCTG TTGGCGGAGA GAGCGATTCT
    GATGATTTAT TTGAACGTAC GGCTACTTCA GGATTCGCCA GTGGTGAAAA ACCTTCAGAT
    TCTGGCGAAC TGGGAGTAAT CGAATTAGCT CAGCGTAGAC GGGAAGACCG GGAAGCGTTT
    AATAATATCA GAAGGGAAGC TGAGAGGCTC GGGAAAACAG TAGAGCGGCT CATAACGGAT
    ACGGATAGTC TTATCCGGGA AGGTCGACTG GTTGTTTTGG GAGGAGATCT TCTTGCGGAG
    GGTGTGGAAT TTAGAGGCGA CGGAGACTCA GCTGAATCTA AACAAGACTC TCAGGTGCTG
    GACTCCATTA ATGCACGCAT GAGGACTTTC CGAACCGATC TCCAGCAATT CATGGATAAA
    GTGGAGCATC TAGGGGATGG TTTACGGGAT CGGGTTGATG ATCTTTCCCC GATGCCTAAC
    CTCACAGAAT CTAGCAGCTT CCTTTCTACG GTTACCTCCA TGTCCAGGGA TTCGCCAATC
    GGGACACTGG GAAGGGATCT AGTGACGGAC TTCCAGTCCG GGCGCAGGGA GGAGCAGGAG
    AGCTGTGTAG ACCGGCCCAG AGGAGCCGCG GGACTCTCTA GATACACCAG ACCACACACT
    TTCAGATCCG AGTTTCGCGA CCCTGTCCTG CCTGAGCTGG GAACGCCAAC CCGAGAGATA
    CAGCTCCGTC TGGACCACGA GCGGAGATTG AGAGCTGGAG CAGAGGACAG AGAAGGCTTT
    ACTTCATCTA TCACTCAGGA GGAGGAGAGG AGGCGTGCAG AGCTCCGAAG AGGACTCGAG
    CTGCAAGGGC TTCAGTCTCA TGAGCCAACA TGGCCACAGG AAAGAGGCCT GCTGCAACAG
    GAAGTGAGGT TGTTCCGCCA CAACATGGTG ATTGTGTACA TGAAGCTGCG CTGGATCCTC
    ATGCACTGGA GACTCGGACG CAGGACGGAC ACCATGGAGG AGGGAGCACA TGCTGAGTAT
    GAACGACTGG AGAATATTCC AGAACTGGGC ATTATGATCG ATCAGGGTGA CGGAGACACG
    GAGCGGGAAG ACAAAGTGCA TCCTCAAATA ACAGGAAGTG ACATTGCAGA GCCAGGACCA
    CTTCTGCTGC CATCGCCGGA GAGATTCCAG TACCAGAAAC AGGCGTGTGA CAGTCGGCGT
    GTTCTGCATG CGCTACGGTC TCTGCTGGAG GAGTTCAGGG CGGAGCTTCG GGAGGAGGCG
    GGGCGGCGCT GGCAGCTGCA GCAGGCGTTA GCCAATGAGA GAGCGGCATG GGAAATACAG
    CGCGCTGAAA TGAGGAGTCA CATCAACACG CTGCAGGATG CCAGAGGGGT TTCTACAGAT
    GCAGATGAAG GTGCAGTTTC TACAGATGTT TTACAGCTGG AGCGGGAAGA GCAGCGCCGC
    CTTCTGGCCG ACAGTCACAG CACGGCACTG GAACTGCGCT GGAAACTGCA GCACGGAGAA
    AAGCGCTGGA CTCGAGAGCG CAACGAACTT CTGGAGCGAT TTGAGAAAGA GCGGCAGGAA
    TGGGACCGCA GCTTACGGGA CATGCACCGC AAGATGGAAA AGATGCAGAA AGATGCTCTT
    CAGTCAGATG AGGGCGCAGC ACATGTCTTC TCCCCTCAGG AAAGCCCTTG TAAGGCTCCC
    AGATCTCCAC GTTCCCCCTG CATTGCAGCA CACACTCGCT CACACTCCGA CTCCGAGGCC
    ACACTGGAGG AGAAGGGAGC AGTAGGAAGA CCCAGGCCCT GTGAGGGACC CCGGCCCCCA
    GAGAGCCTGT TTCTGGATGC CCTCTCCCTG GACCCCCTGA GCGAAGCTGA GGTTCCCCCT
    CCATCACGTC TGGAGAGTGA AAAAAGGTTC CCCCACCTGA AGGAAGTAAG AGACCGAGCA
    CTGAATGAGA TTTCTGAGAT TGCGGATCCT GCCATTTACC CTGAAGAAGA GAAAAGTAGT
    GGCAGCTTAC TCAGGGCAAA GTCAGTCTGC TCCATGAGCG ACTTCCAACG TTTAATGGAC
    AGCTCTCCCT TTCTACCAGA CAAAAGTAAG ACTGGTGAGC ATGGGCGGGA TGATGTGACC
    CCTCCTCTTT CTCCAGACGA CCTCAAGTAC ATTGAGGAGT TTAATAGTAA GGGTTGGGAT
    CTGCCCACCA GCTCTCTAGC CACTTGCCCC ATTCCTGGCC CAGTAATCCC CTCTCCGGTG
    ATGGAGGCCT GGGCGGAGAG AGCAGAATCC CGAAGGGCAG ATTCTACTTC AGAAGCATTC
    CAGCAAGCCT CTTGGTTCTT GACCACCAGC GCTACCTTGA CCACCAACAC CATGAGTAGC
    CCTGAGTACT GCCAAAAGGT CCAACTCAGG GCAGCTCAGG GCAGTGACCA GTTTGGGGTG
    CATGTTCTTC ACAGTCCGTC TCGAGGGGAA CGATCCGCTC CGAGTCCTTC TGAGCAGGAA
    TACATGTTCT CCAAAGTGAC CAAAGTGAAG GCAGGGGAAG TAGTAGGGGG TGCAGAAGAA
    GTATTTGGAG GAAAATGGCC GTGTGAGGTT GGGAGTCATT TGGAGGCAGG ACTTAGGCCT
    GGTGAGCGTC CATCAATTTG TGCTGCAGTC GGCTATACCT CATCACTAGA GCTGGAGCTG
    TCACGCAATC TGAGCGATGA TATGAAGGAG AAGGCATATT CCGCCCGCAA TGCAATCCGA
    TCATCTACTA CCACTGGATC CAGCCCTCCG GAAAGGCAGC TACGGGACAT GGCATGCCAG
    ACGAATGGTC TGACCTCACG AGGAACACAG ACCACTCAGA CTATCAGTGT GGGACTGCAA
    ACTGAGGCTC TTCGCACCCT CACGAGCAGC CCGCATCGCT GTCTTACCCC CAAAGGAGGA
    GGCTCTACTC CCATCTCATC CCCTTCACGT AGCCTAAGAA AGATGCAGTA CTCTCCAGTC
    GTCCAAGCCA AGTTTGAACG GCCATGCTGT TCTCCAAAAT ACGGCTCACC CAAGCTTCAA
    AGGAAACCTT CTAATGCTAG TAAAACAGAA CAGCCTGTCA GCCAAACCCG TGCTCCCACC
    CCAACAACTC CTGTCCAGCA GCAAAAAGGC AATAACGAAT CCGCTTGGGC TCGTTCTACG
    ACCACCAGAG ATAGCCCCGT TCACACCACC ATCAACGACG GCCTCTCCAG CCTCTTCAAC
    ATCATCGACC ACACGCCCAT CGCCTACGAC CCCATGCAGA AGTTCAACAA ATCCCCAAGC
    CGCTCTCGTC CTACCGAATC AGGGCCAACC GAGCCCAAAT CTCCCAGTGT TAGTGCTGCA
    CAGGGGCCTT TGAGGAACTC TCGGGGCCGG TCCCCAAGCC CTGTGCAGCT GATTGTAGAG
    ACGCAGGGCG AGAAAACTCC AGAGGTGATT AGCATCAGAC AGGACCTGTC TGCTCCTCCG
    GGCTACACGT TAGCAGAGAA CGCAGCACGG ATATTGAATA AGAAACTACT GGAGCAGAGC
    TTCAGGGAGG AAAGGAGGCT TTCTGCTTCA TCCGCAGCTG TCCCGAACAG AACCGGAGAC
    TCAGAGAAAC CCGGATGTCT TGAGGTTTCC CCCATGGATG TCCCGCGTGC AGATGATTGC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.