sh3rf2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	sh3rf2
Entrez Gene ID	100537944
Full Name	SH3 domain containing ring finger 2
Synonyms	si:dkey-42a21.3,si:dkey-42a21.4
General protein information	Preferred Names SH3 domain containing ring finger 2 Names putative E3 ubiquitin-protein ligase SH3RF2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009291057.3	XP_009289332.1	putative E3 ubiquitin-protein ligase SH3RF2 isoform X1
XM_009291058.3	XP_009289333.1	putative E3 ubiquitin-protein ligase SH3RF2 isoform X2
XM_009291058.2	XP_009289333.1	putative E3 ubiquitin-protein ligase SH3RF2 isoform X2
XM_009291057.2	XP_009289332.1	putative E3 ubiquitin-protein ligase SH3RF2 isoform X1
XM_021474306.1	XP_021329981.1	putative E3 ubiquitin-protein ligase SH3RF2 isoform X1
XM_021481155.1	XP_021336830.1	putative E3 ubiquitin-protein ligase SH3RF2 isoform X2
XM_021481154.1	XP_021336829.1	putative E3 ubiquitin-protein ligase SH3RF2 isoform X1
XM_021474307.1	XP_021329982.1	putative E3 ubiquitin-protein ligase SH3RF2 isoform X2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC101882901	XP_005173208.1
Danio rerio (zebrafish)	LOC100537944	XP_005173210.1

Gene Ontology

The following sh3rf2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sh3rf2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa19581	XM_009291058.3 Latest version!	Danio rerio SH3 domain containing ring finger 2 (sh3rf2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa19581	XM_021474307.1 Latest version!	Danio rerio SH3 domain containing ring finger 2 (sh3rf2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa19581	XM_021481155.1 Latest version!	Danio rerio SH3 domain containing ring finger 2 (sh3rf2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa19580	XM_009291057.3 Latest version!	Danio rerio SH3 domain containing ring finger 2 (sh3rf2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa19580	XM_021474306.1 Latest version!	Danio rerio SH3 domain containing ring finger 2 (sh3rf2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa19580	XM_021481154.1 Latest version!	Danio rerio SH3 domain containing ring finger 2 (sh3rf2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
ODa19581	XM_009291058.2	Danio rerio SH3 domain containing ring finger 2 (sh3rf2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa19580	XM_009291057.2	Danio rerio SH3 domain containing ring finger 2 (sh3rf2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sh3rf2 ( XM_009291058.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009289333.1 Danio rerio SH3 domain containing ring finger 2 (sh3rf2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa19581
Clone ID Related Accession (Same CDS sequence)	XM_009291058.3 , XM_009291058.2 , XM_021481155.1 , XM_021474307.1
Accession Version	XM_009291058.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	putative E3 ubiquitin-protein ligase SH3RF2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394908.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009291058.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTCTT TTACTTTGGT GGCAGAGCAG CACATCATGG GAAAAGGGAG GGGGAACGTC 
ACTCCTGCCC ACATGTGCTG CACCAGTCCA TCTCAACGCC GCGCTCCTTA CATCCACACT 
CTCAGGAACT TTAAGACGAC TAATAGCTTG AGAATAGCAC GCTTCTTACT GGCAAACTCT 
GAGGCGCAGC AGCCAAATCC GGCGGCATAC GAGCTTCTGG AAAAGCATAA AAGGAGGGAC 
TCAGTGGAAG CGCAGCCAAG GAGCAGCGTT AGGGTCAACG CAGATGCTTG CATCCACAGG 
CGGCTCACTG GTCCATCTCA GAAGTCATCC AGGCCTTCAA ATGTCAATCT CTTGAACAGC 
CTAAACTGTC CTCCCCTCAG CCTGAGCCAG CAGCCTCCTC ACATCAGCTC CAGCGCACAG 
CCATCCTACA GCAAGACGCC ACAACTCGCC ACATTTAACA GACCGCGTCT GAGGAGCTCT 
CGCAGAAACT TGCCTAAGAG ACACAGAACA ATGAACGGTG AAAGTCCGCC GGCCATCACC 
ATGGCACTGA TTAACCCTCA GGCCTCGCCC ATGCCCTCAG AGAGCAAGAT GTCTGCAACT 
CAGCAGCTCT CCATCAGTGT GTGTGCGGCG CTGTATTCCT ACACACCTTA TCGCTCTGAA 
GAGCTGGAAC TGAGGAAAGG TGAAATGGTG GGGGTTTATG GGAAATTCAG CGAGGGCTGG 
CTGCGCGGAC TGTCTCTCAA AACTGGCAAA GTAGGAATCC TGCCTACCAA CTATGTTACA 
CCAGTGCTCA GAACATCAGC AAGGTTTCTC GAACAAAGCA AACCTGCCAT TCCTAATTCA 
AGTGCAGTGT CAACAAAGCG CCACGCCTCA CACAAGCCGC AGGCTGTTGT TCTGGCCCTC 
GACAGGGTAA ACACTGACAG CCCTTCAGCT ACAATGCCTG TGACGTCATC TGCCGGTCCA 
GGAAGAGCAG CACAGCTGGG CGGAAAGCAG GGCTGGAGCT CAGTGAGACG CTCCTTTCAT 
GCCACACATA GGGGACTGCC TCACCATGGA AGCTACAACT CAAACTCTAT CCTAAGCCTG 
CAGCCTCCAC CTCAGGACTT GGGGCAGATC TACACTTTCG GTCGCTCACC TGTTCTTCCT 
AAAAAGAGAA ATGGTTTATT CTCCAACCCC ATGAAGGCAC AGCATTGGGC TTATGAAACA 
ACATCTCCCT CTGCTGGTGC TTATCAACCC ATCATCAACC CTGGCCAGAA GGAGACCACC 
ACAACTCGGC AGTCTATTCT GGTTAAACCA GATGCTTACA AGAACAATGC AGAGAAGCCA 
GTGAAGTCAG TGCGGTTCCT GGCTCATGAC ACTCCTCAGA CAGCACCAAA AACGCCGTCC 
ACTTCCACTG CAGGATTAAG CTCTAGTTCT CAGCCTGGCT TTTCAACTCT GGACCACTGG 
AATCCCTCGG CCATACTGGG TCGAGATGGA CATACATTAG TCCTCAAAGA CACAAAAACC 
CCTCCAGCCA GAAAGAGCAC AGGCCAAGAT CATGCTGCTA TGGACTCCCT CGCGCTAAAT 
ATGAAGCCAG TACTGATCAA CAACCAATCC GGCTCCAGTA GATGTAGGGT AATTAAGGGA 
TACAACGCCA AAACAGATGC AGAGCTCACG CTCACTGAAG GAGAAATGGT CCTGGTTCAG 
AGGCCTCGAG CAGACGGAAG AGTTCTGGTG ACGCAGGAGA TCAGTGGAAA AACAGGCCTT 
TTCCAAAGCA GCGTTCTGGA ACTGCTAGAC AAAATCCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289333.1
CDS	509..2290
Translation	MLSFTLVAEQHIMGKGRGNVTPAHMCCTSPSQRRAPYIHTLRNFKTTNSLRIARFLLANSEAQQPNPAAYELLEKHKRRDSVEAQPRSSVRVNADACIHRRLTGPSQKSSRPSNVNLLNSLNCPPLSLSQQPPHISSSAQPSYSKTPQLATFNRPRLRSSRRNLPKRHRTMNGESPPAITMALINPQASPMPSESKMSATQQLSISVCAALYSYTPYRSEELELRKGEMVGVYGKFSEGWLRGLSLKTGKVGILPTNYVTPVLRTSARFLEQSKPAIPNSSAVSTKRHASHKPQAVVLALDRVNTDSPSATMPVTSSAGPGRAAQLGGKQGWSSVRRSFHATHRGLPHHGSYNSNSILSLQPPPQDLGQIYTFGRSPVLPKKRNGLFSNPMKAQHWAYETTSPSAGAYQPIINPGQKETTTTRQSILVKPDAYKNNAEKPVKSVRFLAHDTPQTAPKTPSTSTAGLSSSSQPGFSTLDHWNPSAILGRDGHTLVLKDTKTPPARKSTGQDHAAMDSLALNMKPVLINNQSGSSRCRVIKGYNAKTDAELTLTEGEMVLVQRPRADGRVLVTQEISGKTGLFQSSVLELLDKIL

Target ORF information:

RefSeq Version	XM_009291058.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SH3 domain containing ring finger 2 (sh3rf2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291058.3

ORF Insert Sequence:

ATGCTTTCTT TTACTTTGGT GGCAGAGCAG CACATCATGG GAAAAGGGAG GGGGAACGTC 
ACTCCTGCCC ACATGTGCTG CACCAGTCCA TCTCAACGCC GCGCTCCTTA CATCCACACT 
CTCAGGAACT TTAAGACGAC TAATAGCTTG AGAATAGCAC GCTTCTTACT GGCAAACTCT 
GAGGCGCAGC AGCCAAATCC GGCGGCATAC GAGCTTCTGG AAAAGCATAA AAGGAGGGAC 
TCAGTGGAAG CGCAGCCAAG GAGCAGCGTT AGGGTCAACG CAGATGCTTG CATCCACAGG 
CGGCTCACTG GTCCATCTCA GAAGTCATCC AGGCCTTCAA ATGTCAATCT CTTGAACAGC 
CTAAACTGTC CTCCCCTCAG CCTGAGCCAG CAGCCTCCTC ACATCAGCTC CAGCGCACAG 
CCATCCTACA GCAAGACGCC ACAACTCGCC ACATTTAACA GACCGCGTCT GAGGAGCTCT 
CGCAGAAACT TGCCTAAGAG ACACAGAACA ATGAACGGTG AAAGTCCGCC GGCCATCACC 
ATGGCACTGA TTAACCCTCA GGCCTCGCCC ATGCCCTCAG AGAGCAAGAT GTCTGCAACT 
CAGCAGCTCT CCATCAGTGT GTGTGCGGCG CTGTATTCCT ACACACCTTA TCGCTCTGAA 
GAGCTGGAAC TGAGGAAAGG TGAAATGGTG GGGGTTTATG GGAAATTCAG CGAGGGCTGG 
CTGCGCGGAC TGTCTCTCAA AACTGGCAAA GTAGGAATCC TGCCTACCAA CTATGTTACA 
CCAGTGCTCA GAACATCAGC AAGGTTTCTC GAACAAAGCA AACCTGCCAT TCCTAATTCA 
AGTGCAGTGT CAACAAAGCG CCACGCCTCA CACAAGCCGC AGGCTGTTGT TCTGGCCCTC 
GACAGGGTAA ACACTGACAG CCCTTCAGCT ACAATGCCTG TGACGTCATC TGCCGGTCCA 
GGAAGAGCAG CACAGCTGGG CGGAAAGCAG GGCTGGAGCT CAGTGAGACG CTCCTTTCAT 
GCCACACATA GGGGACTGCC TCACCATGGA AGCTACAACT CAAACTCTAT CCTAAGCCTG 
CAGCCTCCAC CTCAGGACTT GGGGCAGATC TACACTTTCG GTCGCTCACC TGTTCTTCCT 
AAAAAGAGAA ATGGTTTATT CTCCAACCCC ATGAAGGCAC AGCATTGGGC TTATGAAACA 
ACATCTCCCT CTGCTGGTGC TTATCAACCC ATCATCAACC CTGGCCAGAA GGAGACCACC 
ACAACTCGGC AGTCTATTCT GGTTAAACCA GATGCTTACA AGAACAATGC AGAGAAGCCA 
GTGAAGTCAG TGCGGTTCCT GGCTCATGAC ACTCCTCAGA CAGCACCAAA AACGCCGTCC 
ACTTCCACTG CAGGATTAAG CTCTAGTTCT CAGCCTGGCT TTTCAACTCT GGACCACTGG 
AATCCCTCGG CCATACTGGG TCGAGATGGA CATACATTAG TCCTCAAAGA CACAAAAACC 
CCTCCAGCCA GAAAGAGCAC AGGCCAAGAT CATGCTGCTA TGGACTCCCT CGCGCTAAAT 
ATGAAGCCAG TACTGATCAA CAACCAATCC GGCTCCAGTA GATGTAGGGT AATTAAGGGA 
TACAACGCCA AAACAGATGC AGAGCTCACG CTCACTGAAG GAGAAATGGT CCTGGTTCAG 
AGGCCTCGAG CAGACGGAAG AGTTCTGGTG ACGCAGGAGA TCAGTGGAAA AACAGGCCTT 
TTCCAAAGCA GCGTTCTGGA ACTGCTAGAC AAAATCCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sh3rf2 ( XM_009291058.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009289333.1 Danio rerio SH3 domain containing ring finger 2 (sh3rf2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa19581
Clone ID Related Accession (Same CDS sequence)	XM_009291058.3 , XM_009291058.2 , XM_021481155.1 , XM_021474307.1
Accession Version	XM_009291058.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	putative E3 ubiquitin-protein ligase SH3RF2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291058.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTCTT TTACTTTGGT GGCAGAGCAG CACATCATGG GAAAAGGGAG GGGGAACGTC 
ACTCCTGCCC ACATGTGCTG CACCAGTCCA TCTCAACGCC GCGCTCCTTA CATCCACACT 
CTCAGGAACT TTAAGACGAC TAATAGCTTG AGAATAGCAC GCTTCTTACT GGCAAACTCT 
GAGGCGCAGC AGCCAAATCC GGCGGCATAC GAGCTTCTGG AAAAGCATAA AAGGAGGGAC 
TCAGTGGAAG CGCAGCCAAG GAGCAGCGTT AGGGTCAACG CAGATGCTTG CATCCACAGG 
CGGCTCACTG GTCCATCTCA GAAGTCATCC AGGCCTTCAA ATGTCAATCT CTTGAACAGC 
CTAAACTGTC CTCCCCTCAG CCTGAGCCAG CAGCCTCCTC ACATCAGCTC CAGCGCACAG 
CCATCCTACA GCAAGACGCC ACAACTCGCC ACATTTAACA GACCGCGTCT GAGGAGCTCT 
CGCAGAAACT TGCCTAAGAG ACACAGAACA ATGAACGGTG AAAGTCCGCC GGCCATCACC 
ATGGCACTGA TTAACCCTCA GGCCTCGCCC ATGCCCTCAG AGAGCAAGAT GTCTGCAACT 
CAGCAGCTCT CCATCAGTGT GTGTGCGGCG CTGTATTCCT ACACACCTTA TCGCTCTGAA 
GAGCTGGAAC TGAGGAAAGG TGAAATGGTG GGGGTTTATG GGAAATTCAG CGAGGGCTGG 
CTGCGCGGAC TGTCTCTCAA AACTGGCAAA GTAGGAATCC TGCCTACCAA CTATGTTACA 
CCAGTGCTCA GAACATCAGC AAGGTTTCTC GAACAAAGCA AACCTGCCAT TCCTAATTCA 
AGTGCAGTGT CAACAAAGCG CCACGCCTCA CACAAGCCGC AGGCTGTTGT TCTGGCCCTC 
GACAGGGTAA ACACTGACAG CCCTTCAGCT ACAATGCCTG TGACGTCATC TGCCGGTCCA 
GGAAGAGCAG CACAGCTGGG CGGAAAGCAG GGCTGGAGCT CAGTGAGACG CTCCTTTCAT 
GCCACACATA GGGGACTGCC TCACCATGGA AGCTACAACT CAAACTCTAT CCTAAGCCTG 
CAGCCTCCAC CTCAGGACTT GGGGCAGATC TACACTTTCG GTCGCTCACC TGTTCTTCCT 
AAAAAGAGAA ATGGTTTATT CTCCAACCCC ATGAAGGCAC AGCATTGGGC TTATGAAACA 
ACATCTCCCT CTGCTGGTGC TTATCAACCC ATCATCAACC CTGGCCAGAA GGAGACCACC 
ACAACTCGGC AGTCTATTCT GGTTAAACCA GATGCTTACA AGAACAATGC AGAGAAGCCA 
GTGAAGTCAG TGCGGTTCCT GGCTCATGAC ACTCCTCAGA CAGCACCAAA AACGCCGTCC 
ACTTCCACTG CAGGATTAAG CTCTAGTTCT CAGCCTGGCT TTTCAACTCT GGACCACTGG 
AATCCCTCGG CCATACTGGG TCGAGATGGA CATACATTAG TCCTCAAAGA CACAAAAACC 
CCTCCAGCCA GAAAGAGCAC AGGCCAAGAT CATGCTGCTA TGGACTCCCT CGCGCTAAAT 
ATGAAGCCAG TACTGATCAA CAACCAATCC GGCTCCAGTA GATGTAGGGT AATTAAGGGA 
TACAACGCCA AAACAGATGC AGAGCTCACG CTCACTGAAG GAGAAATGGT CCTGGTTCAG 
AGGCCTCGAG CAGACGGAAG AGTTCTGGTG ACGCAGGAGA TCAGTGGAAA AACAGGCCTT 
TTCCAAAGCA GCGTTCTGGA ACTGCTAGAC AAAATCCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289333.1
CDS	233..2014
Translation	MLSFTLVAEQHIMGKGRGNVTPAHMCCTSPSQRRAPYIHTLRNFKTTNSLRIARFLLANSEAQQPNPAAYELLEKHKRRDSVEAQPRSSVRVNADACIHRRLTGPSQKSSRPSNVNLLNSLNCPPLSLSQQPPHISSSAQPSYSKTPQLATFNRPRLRSSRRNLPKRHRTMNGESPPAITMALINPQASPMPSESKMSATQQLSISVCAALYSYTPYRSEELELRKGEMVGVYGKFSEGWLRGLSLKTGKVGILPTNYVTPVLRTSARFLEQSKPAIPNSSAVSTKRHASHKPQAVVLALDRVNTDSPSATMPVTSSAGPGRAAQLGGKQGWSSVRRSFHATHRGLPHHGSYNSNSILSLQPPPQDLGQIYTFGRSPVLPKKRNGLFSNPMKAQHWAYETTSPSAGAYQPIINPGQKETTTTRQSILVKPDAYKNNAEKPVKSVRFLAHDTPQTAPKTPSTSTAGLSSSSQPGFSTLDHWNPSAILGRDGHTLVLKDTKTPPARKSTGQDHAAMDSLALNMKPVLINNQSGSSRCRVIKGYNAKTDAELTLTEGEMVLVQRPRADGRVLVTQEISGKTGLFQSSVLELLDKIL

Target ORF information:

RefSeq Version	XM_009291058.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio SH3 domain containing ring finger 2 (sh3rf2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291058.2

ORF Insert Sequence:

ATGCTTTCTT TTACTTTGGT GGCAGAGCAG CACATCATGG GAAAAGGGAG GGGGAACGTC 
ACTCCTGCCC ACATGTGCTG CACCAGTCCA TCTCAACGCC GCGCTCCTTA CATCCACACT 
CTCAGGAACT TTAAGACGAC TAATAGCTTG AGAATAGCAC GCTTCTTACT GGCAAACTCT 
GAGGCGCAGC AGCCAAATCC GGCGGCATAC GAGCTTCTGG AAAAGCATAA AAGGAGGGAC 
TCAGTGGAAG CGCAGCCAAG GAGCAGCGTT AGGGTCAACG CAGATGCTTG CATCCACAGG 
CGGCTCACTG GTCCATCTCA GAAGTCATCC AGGCCTTCAA ATGTCAATCT CTTGAACAGC 
CTAAACTGTC CTCCCCTCAG CCTGAGCCAG CAGCCTCCTC ACATCAGCTC CAGCGCACAG 
CCATCCTACA GCAAGACGCC ACAACTCGCC ACATTTAACA GACCGCGTCT GAGGAGCTCT 
CGCAGAAACT TGCCTAAGAG ACACAGAACA ATGAACGGTG AAAGTCCGCC GGCCATCACC 
ATGGCACTGA TTAACCCTCA GGCCTCGCCC ATGCCCTCAG AGAGCAAGAT GTCTGCAACT 
CAGCAGCTCT CCATCAGTGT GTGTGCGGCG CTGTATTCCT ACACACCTTA TCGCTCTGAA 
GAGCTGGAAC TGAGGAAAGG TGAAATGGTG GGGGTTTATG GGAAATTCAG CGAGGGCTGG 
CTGCGCGGAC TGTCTCTCAA AACTGGCAAA GTAGGAATCC TGCCTACCAA CTATGTTACA 
CCAGTGCTCA GAACATCAGC AAGGTTTCTC GAACAAAGCA AACCTGCCAT TCCTAATTCA 
AGTGCAGTGT CAACAAAGCG CCACGCCTCA CACAAGCCGC AGGCTGTTGT TCTGGCCCTC 
GACAGGGTAA ACACTGACAG CCCTTCAGCT ACAATGCCTG TGACGTCATC TGCCGGTCCA 
GGAAGAGCAG CACAGCTGGG CGGAAAGCAG GGCTGGAGCT CAGTGAGACG CTCCTTTCAT 
GCCACACATA GGGGACTGCC TCACCATGGA AGCTACAACT CAAACTCTAT CCTAAGCCTG 
CAGCCTCCAC CTCAGGACTT GGGGCAGATC TACACTTTCG GTCGCTCACC TGTTCTTCCT 
AAAAAGAGAA ATGGTTTATT CTCCAACCCC ATGAAGGCAC AGCATTGGGC TTATGAAACA 
ACATCTCCCT CTGCTGGTGC TTATCAACCC ATCATCAACC CTGGCCAGAA GGAGACCACC 
ACAACTCGGC AGTCTATTCT GGTTAAACCA GATGCTTACA AGAACAATGC AGAGAAGCCA 
GTGAAGTCAG TGCGGTTCCT GGCTCATGAC ACTCCTCAGA CAGCACCAAA AACGCCGTCC 
ACTTCCACTG CAGGATTAAG CTCTAGTTCT CAGCCTGGCT TTTCAACTCT GGACCACTGG 
AATCCCTCGG CCATACTGGG TCGAGATGGA CATACATTAG TCCTCAAAGA CACAAAAACC 
CCTCCAGCCA GAAAGAGCAC AGGCCAAGAT CATGCTGCTA TGGACTCCCT CGCGCTAAAT 
ATGAAGCCAG TACTGATCAA CAACCAATCC GGCTCCAGTA GATGTAGGGT AATTAAGGGA 
TACAACGCCA AAACAGATGC AGAGCTCACG CTCACTGAAG GAGAAATGGT CCTGGTTCAG 
AGGCCTCGAG CAGACGGAAG AGTTCTGGTG ACGCAGGAGA TCAGTGGAAA AACAGGCCTT 
TTCCAAAGCA GCGTTCTGGA ACTGCTAGAC AAAATCCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sh3rf2 ( XM_021481155.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021336830.1 Danio rerio SH3 domain containing ring finger 2 (sh3rf2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa19581
Clone ID Related Accession (Same CDS sequence)	XM_009291058.3 , XM_009291058.2 , XM_021481155.1 , XM_021474307.1
Accession Version	XM_021481155.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	putative E3 ubiquitin-protein ligase SH3RF2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTCTT TTACTTTGGT GGCAGAGCAG CACATCATGG GAAAAGGGAG GGGGAACGTC 
ACTCCTGCCC ACATGTGCTG CACCAGTCCA TCTCAACGCC GCGCTCCTTA CATCCACACT 
CTCAGGAACT TTAAGACGAC TAATAGCTTG AGAATAGCAC GCTTCTTACT GGCAAACTCT 
GAGGCGCAGC AGCCAAATCC GGCGGCATAC GAGCTTCTGG AAAAGCATAA AAGGAGGGAC 
TCAGTGGAAG CGCAGCCAAG GAGCAGCGTT AGGGTCAACG CAGATGCTTG CATCCACAGG 
CGGCTCACTG GTCCATCTCA GAAGTCATCC AGGCCTTCAA ATGTCAATCT CTTGAACAGC 
CTAAACTGTC CTCCCCTCAG CCTGAGCCAG CAGCCTCCTC ACATCAGCTC CAGCGCACAG 
CCATCCTACA GCAAGACGCC ACAACTCGCC ACATTTAACA GACCGCGTCT GAGGAGCTCT 
CGCAGAAACT TGCCTAAGAG ACACAGAACA ATGAACGGTG AAAGTCCGCC GGCCATCACC 
ATGGCACTGA TTAACCCTCA GGCCTCGCCC ATGCCCTCAG AGAGCAAGAT GTCTGCAACT 
CAGCAGCTCT CCATCAGTGT GTGTGCGGCG CTGTATTCCT ACACACCTTA TCGCTCTGAA 
GAGCTGGAAC TGAGGAAAGG TGAAATGGTG GGGGTTTATG GGAAATTCAG CGAGGGCTGG 
CTGCGCGGAC TGTCTCTCAA AACTGGCAAA GTAGGAATCC TGCCTACCAA CTATGTTACA 
CCAGTGCTCA GAACATCAGC AAGGTTTCTC GAACAAAGCA AACCTGCCAT TCCTAATTCA 
AGTGCAGTGT CAACAAAGCG CCACGCCTCA CACAAGCCGC AGGCTGTTGT TCTGGCCCTC 
GACAGGGTAA ACACTGACAG CCCTTCAGCT ACAATGCCTG TGACGTCATC TGCCGGTCCA 
GGAAGAGCAG CACAGCTGGG CGGAAAGCAG GGCTGGAGCT CAGTGAGACG CTCCTTTCAT 
GCCACACATA GGGGACTGCC TCACCATGGA AGCTACAACT CAAACTCTAT CCTAAGCCTG 
CAGCCTCCAC CTCAGGACTT GGGGCAGATC TACACTTTCG GTCGCTCACC TGTTCTTCCT 
AAAAAGAGAA ATGGTTTATT CTCCAACCCC ATGAAGGCAC AGCATTGGGC TTATGAAACA 
ACATCTCCCT CTGCTGGTGC TTATCAACCC ATCATCAACC CTGGCCAGAA GGAGACCACC 
ACAACTCGGC AGTCTATTCT GGTTAAACCA GATGCTTACA AGAACAATGC AGAGAAGCCA 
GTGAAGTCAG TGCGGTTCCT GGCTCATGAC ACTCCTCAGA CAGCACCAAA AACGCCGTCC 
ACTTCCACTG CAGGATTAAG CTCTAGTTCT CAGCCTGGCT TTTCAACTCT GGACCACTGG 
AATCCCTCGG CCATACTGGG TCGAGATGGA CATACATTAG TCCTCAAAGA CACAAAAACC 
CCTCCAGCCA GAAAGAGCAC AGGCCAAGAT CATGCTGCTA TGGACTCCCT CGCGCTAAAT 
ATGAAGCCAG TACTGATCAA CAACCAATCC GGCTCCAGTA GATGTAGGGT AATTAAGGGA 
TACAACGCCA AAACAGATGC AGAGCTCACG CTCACTGAAG GAGAAATGGT CCTGGTTCAG 
AGGCCTCGAG CAGACGGAAG AGTTCTGGTG ACGCAGGAGA TCAGTGGAAA AACAGGCCTT 
TTCCAAAGCA GCGTTCTGGA ACTGCTAGAC AAAATCCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336830.1
CDS	509..2290
Translation	MLSFTLVAEQHIMGKGRGNVTPAHMCCTSPSQRRAPYIHTLRNFKTTNSLRIARFLLANSEAQQPNPAAYELLEKHKRRDSVEAQPRSSVRVNADACIHRRLTGPSQKSSRPSNVNLLNSLNCPPLSLSQQPPHISSSAQPSYSKTPQLATFNRPRLRSSRRNLPKRHRTMNGESPPAITMALINPQASPMPSESKMSATQQLSISVCAALYSYTPYRSEELELRKGEMVGVYGKFSEGWLRGLSLKTGKVGILPTNYVTPVLRTSARFLEQSKPAIPNSSAVSTKRHASHKPQAVVLALDRVNTDSPSATMPVTSSAGPGRAAQLGGKQGWSSVRRSFHATHRGLPHHGSYNSNSILSLQPPPQDLGQIYTFGRSPVLPKKRNGLFSNPMKAQHWAYETTSPSAGAYQPIINPGQKETTTTRQSILVKPDAYKNNAEKPVKSVRFLAHDTPQTAPKTPSTSTAGLSSSSQPGFSTLDHWNPSAILGRDGHTLVLKDTKTPPARKSTGQDHAAMDSLALNMKPVLINNQSGSSRCRVIKGYNAKTDAELTLTEGEMVLVQRPRADGRVLVTQEISGKTGLFQSSVLELLDKIL

Target ORF information:

RefSeq Version	XM_021481155.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SH3 domain containing ring finger 2 (sh3rf2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481155.1

ORF Insert Sequence:

ATGCTTTCTT TTACTTTGGT GGCAGAGCAG CACATCATGG GAAAAGGGAG GGGGAACGTC 
ACTCCTGCCC ACATGTGCTG CACCAGTCCA TCTCAACGCC GCGCTCCTTA CATCCACACT 
CTCAGGAACT TTAAGACGAC TAATAGCTTG AGAATAGCAC GCTTCTTACT GGCAAACTCT 
GAGGCGCAGC AGCCAAATCC GGCGGCATAC GAGCTTCTGG AAAAGCATAA AAGGAGGGAC 
TCAGTGGAAG CGCAGCCAAG GAGCAGCGTT AGGGTCAACG CAGATGCTTG CATCCACAGG 
CGGCTCACTG GTCCATCTCA GAAGTCATCC AGGCCTTCAA ATGTCAATCT CTTGAACAGC 
CTAAACTGTC CTCCCCTCAG CCTGAGCCAG CAGCCTCCTC ACATCAGCTC CAGCGCACAG 
CCATCCTACA GCAAGACGCC ACAACTCGCC ACATTTAACA GACCGCGTCT GAGGAGCTCT 
CGCAGAAACT TGCCTAAGAG ACACAGAACA ATGAACGGTG AAAGTCCGCC GGCCATCACC 
ATGGCACTGA TTAACCCTCA GGCCTCGCCC ATGCCCTCAG AGAGCAAGAT GTCTGCAACT 
CAGCAGCTCT CCATCAGTGT GTGTGCGGCG CTGTATTCCT ACACACCTTA TCGCTCTGAA 
GAGCTGGAAC TGAGGAAAGG TGAAATGGTG GGGGTTTATG GGAAATTCAG CGAGGGCTGG 
CTGCGCGGAC TGTCTCTCAA AACTGGCAAA GTAGGAATCC TGCCTACCAA CTATGTTACA 
CCAGTGCTCA GAACATCAGC AAGGTTTCTC GAACAAAGCA AACCTGCCAT TCCTAATTCA 
AGTGCAGTGT CAACAAAGCG CCACGCCTCA CACAAGCCGC AGGCTGTTGT TCTGGCCCTC 
GACAGGGTAA ACACTGACAG CCCTTCAGCT ACAATGCCTG TGACGTCATC TGCCGGTCCA 
GGAAGAGCAG CACAGCTGGG CGGAAAGCAG GGCTGGAGCT CAGTGAGACG CTCCTTTCAT 
GCCACACATA GGGGACTGCC TCACCATGGA AGCTACAACT CAAACTCTAT CCTAAGCCTG 
CAGCCTCCAC CTCAGGACTT GGGGCAGATC TACACTTTCG GTCGCTCACC TGTTCTTCCT 
AAAAAGAGAA ATGGTTTATT CTCCAACCCC ATGAAGGCAC AGCATTGGGC TTATGAAACA 
ACATCTCCCT CTGCTGGTGC TTATCAACCC ATCATCAACC CTGGCCAGAA GGAGACCACC 
ACAACTCGGC AGTCTATTCT GGTTAAACCA GATGCTTACA AGAACAATGC AGAGAAGCCA 
GTGAAGTCAG TGCGGTTCCT GGCTCATGAC ACTCCTCAGA CAGCACCAAA AACGCCGTCC 
ACTTCCACTG CAGGATTAAG CTCTAGTTCT CAGCCTGGCT TTTCAACTCT GGACCACTGG 
AATCCCTCGG CCATACTGGG TCGAGATGGA CATACATTAG TCCTCAAAGA CACAAAAACC 
CCTCCAGCCA GAAAGAGCAC AGGCCAAGAT CATGCTGCTA TGGACTCCCT CGCGCTAAAT 
ATGAAGCCAG TACTGATCAA CAACCAATCC GGCTCCAGTA GATGTAGGGT AATTAAGGGA 
TACAACGCCA AAACAGATGC AGAGCTCACG CTCACTGAAG GAGAAATGGT CCTGGTTCAG 
AGGCCTCGAG CAGACGGAAG AGTTCTGGTG ACGCAGGAGA TCAGTGGAAA AACAGGCCTT 
TTCCAAAGCA GCGTTCTGGA ACTGCTAGAC AAAATCCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sh3rf2 ( XM_021474307.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329982.1 Danio rerio SH3 domain containing ring finger 2 (sh3rf2), transcript variant X2, mRNA.

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CloneID	ODa19581
Clone ID Related Accession (Same CDS sequence)	XM_009291058.3 , XM_009291058.2 , XM_021481155.1 , XM_021474307.1
Accession Version	XM_021474307.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	putative E3 ubiquitin-protein ligase SH3RF2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395314.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTCTT TTACTTTGGT GGCAGAGCAG CACATCATGG GAAAAGGGAG GGGGAACGTC 
ACTCCTGCCC ACATGTGCTG CACCAGTCCA TCTCAACGCC GCGCTCCTTA CATCCACACT 
CTCAGGAACT TTAAGACGAC TAATAGCTTG AGAATAGCAC GCTTCTTACT GGCAAACTCT 
GAGGCGCAGC AGCCAAATCC GGCGGCATAC GAGCTTCTGG AAAAGCATAA AAGGAGGGAC 
TCAGTGGAAG CGCAGCCAAG GAGCAGCGTT AGGGTCAACG CAGATGCTTG CATCCACAGG 
CGGCTCACTG GTCCATCTCA GAAGTCATCC AGGCCTTCAA ATGTCAATCT CTTGAACAGC 
CTAAACTGTC CTCCCCTCAG CCTGAGCCAG CAGCCTCCTC ACATCAGCTC CAGCGCACAG 
CCATCCTACA GCAAGACGCC ACAACTCGCC ACATTTAACA GACCGCGTCT GAGGAGCTCT 
CGCAGAAACT TGCCTAAGAG ACACAGAACA ATGAACGGTG AAAGTCCGCC GGCCATCACC 
ATGGCACTGA TTAACCCTCA GGCCTCGCCC ATGCCCTCAG AGAGCAAGAT GTCTGCAACT 
CAGCAGCTCT CCATCAGTGT GTGTGCGGCG CTGTATTCCT ACACACCTTA TCGCTCTGAA 
GAGCTGGAAC TGAGGAAAGG TGAAATGGTG GGGGTTTATG GGAAATTCAG CGAGGGCTGG 
CTGCGCGGAC TGTCTCTCAA AACTGGCAAA GTAGGAATCC TGCCTACCAA CTATGTTACA 
CCAGTGCTCA GAACATCAGC AAGGTTTCTC GAACAAAGCA AACCTGCCAT TCCTAATTCA 
AGTGCAGTGT CAACAAAGCG CCACGCCTCA CACAAGCCGC AGGCTGTTGT TCTGGCCCTC 
GACAGGGTAA ACACTGACAG CCCTTCAGCT ACAATGCCTG TGACGTCATC TGCCGGTCCA 
GGAAGAGCAG CACAGCTGGG CGGAAAGCAG GGCTGGAGCT CAGTGAGACG CTCCTTTCAT 
GCCACACATA GGGGACTGCC TCACCATGGA AGCTACAACT CAAACTCTAT CCTAAGCCTG 
CAGCCTCCAC CTCAGGACTT GGGGCAGATC TACACTTTCG GTCGCTCACC TGTTCTTCCT 
AAAAAGAGAA ATGGTTTATT CTCCAACCCC ATGAAGGCAC AGCATTGGGC TTATGAAACA 
ACATCTCCCT CTGCTGGTGC TTATCAACCC ATCATCAACC CTGGCCAGAA GGAGACCACC 
ACAACTCGGC AGTCTATTCT GGTTAAACCA GATGCTTACA AGAACAATGC AGAGAAGCCA 
GTGAAGTCAG TGCGGTTCCT GGCTCATGAC ACTCCTCAGA CAGCACCAAA AACGCCGTCC 
ACTTCCACTG CAGGATTAAG CTCTAGTTCT CAGCCTGGCT TTTCAACTCT GGACCACTGG 
AATCCCTCGG CCATACTGGG TCGAGATGGA CATACATTAG TCCTCAAAGA CACAAAAACC 
CCTCCAGCCA GAAAGAGCAC AGGCCAAGAT CATGCTGCTA TGGACTCCCT CGCGCTAAAT 
ATGAAGCCAG TACTGATCAA CAACCAATCC GGCTCCAGTA GATGTAGGGT AATTAAGGGA 
TACAACGCCA AAACAGATGC AGAGCTCACG CTCACTGAAG GAGAAATGGT CCTGGTTCAG 
AGGCCTCGAG CAGACGGAAG AGTTCTGGTG ACGCAGGAGA TCAGTGGAAA AACAGGCCTT 
TTCCAAAGCA GCGTTCTGGA ACTGCTAGAC AAAATCCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329982.1
CDS	509..2290
Translation	MLSFTLVAEQHIMGKGRGNVTPAHMCCTSPSQRRAPYIHTLRNFKTTNSLRIARFLLANSEAQQPNPAAYELLEKHKRRDSVEAQPRSSVRVNADACIHRRLTGPSQKSSRPSNVNLLNSLNCPPLSLSQQPPHISSSAQPSYSKTPQLATFNRPRLRSSRRNLPKRHRTMNGESPPAITMALINPQASPMPSESKMSATQQLSISVCAALYSYTPYRSEELELRKGEMVGVYGKFSEGWLRGLSLKTGKVGILPTNYVTPVLRTSARFLEQSKPAIPNSSAVSTKRHASHKPQAVVLALDRVNTDSPSATMPVTSSAGPGRAAQLGGKQGWSSVRRSFHATHRGLPHHGSYNSNSILSLQPPPQDLGQIYTFGRSPVLPKKRNGLFSNPMKAQHWAYETTSPSAGAYQPIINPGQKETTTTRQSILVKPDAYKNNAEKPVKSVRFLAHDTPQTAPKTPSTSTAGLSSSSQPGFSTLDHWNPSAILGRDGHTLVLKDTKTPPARKSTGQDHAAMDSLALNMKPVLINNQSGSSRCRVIKGYNAKTDAELTLTEGEMVLVQRPRADGRVLVTQEISGKTGLFQSSVLELLDKIL

Target ORF information:

RefSeq Version	XM_021474307.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SH3 domain containing ring finger 2 (sh3rf2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474307.1

ORF Insert Sequence:

ATGCTTTCTT TTACTTTGGT GGCAGAGCAG CACATCATGG GAAAAGGGAG GGGGAACGTC 
ACTCCTGCCC ACATGTGCTG CACCAGTCCA TCTCAACGCC GCGCTCCTTA CATCCACACT 
CTCAGGAACT TTAAGACGAC TAATAGCTTG AGAATAGCAC GCTTCTTACT GGCAAACTCT 
GAGGCGCAGC AGCCAAATCC GGCGGCATAC GAGCTTCTGG AAAAGCATAA AAGGAGGGAC 
TCAGTGGAAG CGCAGCCAAG GAGCAGCGTT AGGGTCAACG CAGATGCTTG CATCCACAGG 
CGGCTCACTG GTCCATCTCA GAAGTCATCC AGGCCTTCAA ATGTCAATCT CTTGAACAGC 
CTAAACTGTC CTCCCCTCAG CCTGAGCCAG CAGCCTCCTC ACATCAGCTC CAGCGCACAG 
CCATCCTACA GCAAGACGCC ACAACTCGCC ACATTTAACA GACCGCGTCT GAGGAGCTCT 
CGCAGAAACT TGCCTAAGAG ACACAGAACA ATGAACGGTG AAAGTCCGCC GGCCATCACC 
ATGGCACTGA TTAACCCTCA GGCCTCGCCC ATGCCCTCAG AGAGCAAGAT GTCTGCAACT 
CAGCAGCTCT CCATCAGTGT GTGTGCGGCG CTGTATTCCT ACACACCTTA TCGCTCTGAA 
GAGCTGGAAC TGAGGAAAGG TGAAATGGTG GGGGTTTATG GGAAATTCAG CGAGGGCTGG 
CTGCGCGGAC TGTCTCTCAA AACTGGCAAA GTAGGAATCC TGCCTACCAA CTATGTTACA 
CCAGTGCTCA GAACATCAGC AAGGTTTCTC GAACAAAGCA AACCTGCCAT TCCTAATTCA 
AGTGCAGTGT CAACAAAGCG CCACGCCTCA CACAAGCCGC AGGCTGTTGT TCTGGCCCTC 
GACAGGGTAA ACACTGACAG CCCTTCAGCT ACAATGCCTG TGACGTCATC TGCCGGTCCA 
GGAAGAGCAG CACAGCTGGG CGGAAAGCAG GGCTGGAGCT CAGTGAGACG CTCCTTTCAT 
GCCACACATA GGGGACTGCC TCACCATGGA AGCTACAACT CAAACTCTAT CCTAAGCCTG 
CAGCCTCCAC CTCAGGACTT GGGGCAGATC TACACTTTCG GTCGCTCACC TGTTCTTCCT 
AAAAAGAGAA ATGGTTTATT CTCCAACCCC ATGAAGGCAC AGCATTGGGC TTATGAAACA 
ACATCTCCCT CTGCTGGTGC TTATCAACCC ATCATCAACC CTGGCCAGAA GGAGACCACC 
ACAACTCGGC AGTCTATTCT GGTTAAACCA GATGCTTACA AGAACAATGC AGAGAAGCCA 
GTGAAGTCAG TGCGGTTCCT GGCTCATGAC ACTCCTCAGA CAGCACCAAA AACGCCGTCC 
ACTTCCACTG CAGGATTAAG CTCTAGTTCT CAGCCTGGCT TTTCAACTCT GGACCACTGG 
AATCCCTCGG CCATACTGGG TCGAGATGGA CATACATTAG TCCTCAAAGA CACAAAAACC 
CCTCCAGCCA GAAAGAGCAC AGGCCAAGAT CATGCTGCTA TGGACTCCCT CGCGCTAAAT 
ATGAAGCCAG TACTGATCAA CAACCAATCC GGCTCCAGTA GATGTAGGGT AATTAAGGGA 
TACAACGCCA AAACAGATGC AGAGCTCACG CTCACTGAAG GAGAAATGGT CCTGGTTCAG 
AGGCCTCGAG CAGACGGAAG AGTTCTGGTG ACGCAGGAGA TCAGTGGAAA AACAGGCCTT 
TTCCAAAGCA GCGTTCTGGA ACTGCTAGAC AAAATCCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sh3rf2 ( XM_009291057.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009289332.1 Danio rerio SH3 domain containing ring finger 2 (sh3rf2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa19580
Clone ID Related Accession (Same CDS sequence)	XM_009291057.3 , XM_009291057.2 , XM_021474306.1 , XM_021481154.1
Accession Version	XM_009291057.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	putative E3 ubiquitin-protein ligase SH3RF2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394908.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009291057.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGATC TGGCTGTTAT GGAGCTGTTA GAGTGTCCTT TGTGTATGGA GCCATTGGAC 
GTGTCTGCAA AGGTCCTGCC ATGCCAGCAC ACGTTCTGTA AATCATGCCT TCAGCAGCAG 
GAGACATCAC ACCCTGATCA GTTGTGCTGC CCAGAGTGCA GAGCTGCTGT TCCTGGATCA 
GTGGAGGAGC TTCCCACTAA CCCGCTGCTG CATAGACTCT TAGAGAGCCT TCACGAATGG 
GGTCCAGTGA GCACACCGAG GGACAGATCT GTCAGATATA TGAGCTCTCC GGCTCAGGAG 
GACCATTGCA GCAGTTACAC GCAAGATCTT CCCAAGCAGG AGCAGAACAC GCAGAGGGTT 
CAAGCCCAAG CATTATATGA CTTCCAGGGT AATGCTCCAG GGGAGCTCAC AATGAAGTCA 
GGTGACATCA TTTATCTGCG GTGGAGAGTA GATGATAACT GGTATTATGG GGAAGGCAAA 
GACAGCAGAG GTTTGGTTCC AGTCAATGTG TTGCAGGTCT TGAGTGAACA GCCTCAGCCT 
CTAGCCCTCT GCAGAGCTCT TTATGACTTC AATGCTAACA ATCTGGATCC AGAGGGCAGC 
AAGGAATGCT TAACCTTCTT CAAGGGAGAC AGCATTACTC TCCTTAAACA AGTCAATGAA 
AATTGGGTGG AAGGTAAACT CGGAGACAAA GTTGGGATTT TTCCTTTGCA ACTCACAGAG 
CCAAATCCGG CGGCATACGA GCTTCTGGAA AAGCATAAAA GGAGGGACTC AGTGGAAGCG 
CAGCCAAGGA GCAGCGTTAG GGTCAACGCA GATGCTTGCA TCCACAGGCG GCTCACTGGT 
CCATCTCAGA AGTCATCCAG GCCTTCAAAT GTCAATCTCT TGAACAGCCT AAACTGTCCT 
CCCCTCAGCC TGAGCCAGCA GCCTCCTCAC ATCAGCTCCA GCGCACAGCC ATCCTACAGC 
AAGACGCCAC AACTCGCCAC ATTTAACAGA CCGCGTCTGA GGAGCTCTCG CAGAAACTTG 
CCTAAGAGAC ACAGAACAAT GAACGGTGAA AGTCCGCCGG CCATCACCAT GGCACTGATT 
AACCCTCAGG CCTCGCCCAT GCCCTCAGAG AGCAAGATGT CTGCAACTCA GCAGCTCTCC 
ATCAGTGTGT GTGCGGCGCT GTATTCCTAC ACACCTTATC GCTCTGAAGA GCTGGAACTG 
AGGAAAGGTG AAATGGTGGG GGTTTATGGG AAATTCAGCG AGGGCTGGCT GCGCGGACTG 
TCTCTCAAAA CTGGCAAAGT AGGAATCCTG CCTACCAACT ATGTTACACC AGTGCTCAGA 
ACATCAGCAA GGTTTCTCGA ACAAAGCAAA CCTGCCATTC CTAATTCAAG TGCAGTGTCA 
ACAAAGCGCC ACGCCTCACA CAAGCCGCAG GCTGTTGTTC TGGCCCTCGA CAGGGTAAAC 
ACTGACAGCC CTTCAGCTAC AATGCCTGTG ACGTCATCTG CCGGTCCAGG AAGAGCAGCA 
CAGCTGGGCG GAAAGCAGGG CTGGAGCTCA GTGAGACGCT CCTTTCATGC CACACATAGG 
GGACTGCCTC ACCATGGAAG CTACAACTCA AACTCTATCC TAAGCCTGCA GCCTCCACCT 
CAGGACTTGG GGCAGATCTA CACTTTCGGT CGCTCACCTG TTCTTCCTAA AAAGAGAAAT 
GGTTTATTCT CCAACCCCAT GAAGGCACAG CATTGGGCTT ATGAAACAAC ATCTCCCTCT 
GCTGGTGCTT ATCAACCCAT CATCAACCCT GGCCAGAAGG AGACCACCAC AACTCGGCAG 
TCTATTCTGG TTAAACCAGA TGCTTACAAG AACAATGCAG AGAAGCCAGT GAAGTCAGTG 
CGGTTCCTGG CTCATGACAC TCCTCAGACA GCACCAAAAA CGCCGTCCAC TTCCACTGCA 
GGATTAAGCT CTAGTTCTCA GCCTGGCTTT TCAACTCTGG ACCACTGGAA TCCCTCGGCC 
ATACTGGGTC GAGATGGACA TACATTAGTC CTCAAAGACA CAAAAACCCC TCCAGCCAGA 
AAGAGCACAG GCCAAGATCA TGCTGCTATG GACTCCCTCG CGCTAAATAT GAAGCCAGTA 
CTGATCAACA ACCAATCCGG CTCCAGTAGA TGTAGGGTAA TTAAGGGATA CAACGCCAAA 
ACAGATGCAG AGCTCACGCT CACTGAAGGA GAAATGGTCC TGGTTCAGAG GCCTCGAGCA 
GACGGAAGAG TTCTGGTGAC GCAGGAGATC AGTGGAAAAA CAGGCCTTTT CCAAAGCAGC 
GTTCTGGAAC TGCTAGACAA AATCCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289332.1
CDS	144..2453
Translation	MEDLAVMELLECPLCMEPLDVSAKVLPCQHTFCKSCLQQQETSHPDQLCCPECRAAVPGSVEELPTNPLLHRLLESLHEWGPVSTPRDRSVRYMSSPAQEDHCSSYTQDLPKQEQNTQRVQAQALYDFQGNAPGELTMKSGDIIYLRWRVDDNWYYGEGKDSRGLVPVNVLQVLSEQPQPLALCRALYDFNANNLDPEGSKECLTFFKGDSITLLKQVNENWVEGKLGDKVGIFPLQLTEPNPAAYELLEKHKRRDSVEAQPRSSVRVNADACIHRRLTGPSQKSSRPSNVNLLNSLNCPPLSLSQQPPHISSSAQPSYSKTPQLATFNRPRLRSSRRNLPKRHRTMNGESPPAITMALINPQASPMPSESKMSATQQLSISVCAALYSYTPYRSEELELRKGEMVGVYGKFSEGWLRGLSLKTGKVGILPTNYVTPVLRTSARFLEQSKPAIPNSSAVSTKRHASHKPQAVVLALDRVNTDSPSATMPVTSSAGPGRAAQLGGKQGWSSVRRSFHATHRGLPHHGSYNSNSILSLQPPPQDLGQIYTFGRSPVLPKKRNGLFSNPMKAQHWAYETTSPSAGAYQPIINPGQKETTTTRQSILVKPDAYKNNAEKPVKSVRFLAHDTPQTAPKTPSTSTAGLSSSSQPGFSTLDHWNPSAILGRDGHTLVLKDTKTPPARKSTGQDHAAMDSLALNMKPVLINNQSGSSRCRVIKGYNAKTDAELTLTEGEMVLVQRPRADGRVLVTQEISGKTGLFQSSVLELLDKIL

Target ORF information:

RefSeq Version	XM_009291057.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SH3 domain containing ring finger 2 (sh3rf2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291057.3

ORF Insert Sequence:

ATGGAGGATC TGGCTGTTAT GGAGCTGTTA GAGTGTCCTT TGTGTATGGA GCCATTGGAC 
GTGTCTGCAA AGGTCCTGCC ATGCCAGCAC ACGTTCTGTA AATCATGCCT TCAGCAGCAG 
GAGACATCAC ACCCTGATCA GTTGTGCTGC CCAGAGTGCA GAGCTGCTGT TCCTGGATCA 
GTGGAGGAGC TTCCCACTAA CCCGCTGCTG CATAGACTCT TAGAGAGCCT TCACGAATGG 
GGTCCAGTGA GCACACCGAG GGACAGATCT GTCAGATATA TGAGCTCTCC GGCTCAGGAG 
GACCATTGCA GCAGTTACAC GCAAGATCTT CCCAAGCAGG AGCAGAACAC GCAGAGGGTT 
CAAGCCCAAG CATTATATGA CTTCCAGGGT AATGCTCCAG GGGAGCTCAC AATGAAGTCA 
GGTGACATCA TTTATCTGCG GTGGAGAGTA GATGATAACT GGTATTATGG GGAAGGCAAA 
GACAGCAGAG GTTTGGTTCC AGTCAATGTG TTGCAGGTCT TGAGTGAACA GCCTCAGCCT 
CTAGCCCTCT GCAGAGCTCT TTATGACTTC AATGCTAACA ATCTGGATCC AGAGGGCAGC 
AAGGAATGCT TAACCTTCTT CAAGGGAGAC AGCATTACTC TCCTTAAACA AGTCAATGAA 
AATTGGGTGG AAGGTAAACT CGGAGACAAA GTTGGGATTT TTCCTTTGCA ACTCACAGAG 
CCAAATCCGG CGGCATACGA GCTTCTGGAA AAGCATAAAA GGAGGGACTC AGTGGAAGCG 
CAGCCAAGGA GCAGCGTTAG GGTCAACGCA GATGCTTGCA TCCACAGGCG GCTCACTGGT 
CCATCTCAGA AGTCATCCAG GCCTTCAAAT GTCAATCTCT TGAACAGCCT AAACTGTCCT 
CCCCTCAGCC TGAGCCAGCA GCCTCCTCAC ATCAGCTCCA GCGCACAGCC ATCCTACAGC 
AAGACGCCAC AACTCGCCAC ATTTAACAGA CCGCGTCTGA GGAGCTCTCG CAGAAACTTG 
CCTAAGAGAC ACAGAACAAT GAACGGTGAA AGTCCGCCGG CCATCACCAT GGCACTGATT 
AACCCTCAGG CCTCGCCCAT GCCCTCAGAG AGCAAGATGT CTGCAACTCA GCAGCTCTCC 
ATCAGTGTGT GTGCGGCGCT GTATTCCTAC ACACCTTATC GCTCTGAAGA GCTGGAACTG 
AGGAAAGGTG AAATGGTGGG GGTTTATGGG AAATTCAGCG AGGGCTGGCT GCGCGGACTG 
TCTCTCAAAA CTGGCAAAGT AGGAATCCTG CCTACCAACT ATGTTACACC AGTGCTCAGA 
ACATCAGCAA GGTTTCTCGA ACAAAGCAAA CCTGCCATTC CTAATTCAAG TGCAGTGTCA 
ACAAAGCGCC ACGCCTCACA CAAGCCGCAG GCTGTTGTTC TGGCCCTCGA CAGGGTAAAC 
ACTGACAGCC CTTCAGCTAC AATGCCTGTG ACGTCATCTG CCGGTCCAGG AAGAGCAGCA 
CAGCTGGGCG GAAAGCAGGG CTGGAGCTCA GTGAGACGCT CCTTTCATGC CACACATAGG 
GGACTGCCTC ACCATGGAAG CTACAACTCA AACTCTATCC TAAGCCTGCA GCCTCCACCT 
CAGGACTTGG GGCAGATCTA CACTTTCGGT CGCTCACCTG TTCTTCCTAA AAAGAGAAAT 
GGTTTATTCT CCAACCCCAT GAAGGCACAG CATTGGGCTT ATGAAACAAC ATCTCCCTCT 
GCTGGTGCTT ATCAACCCAT CATCAACCCT GGCCAGAAGG AGACCACCAC AACTCGGCAG 
TCTATTCTGG TTAAACCAGA TGCTTACAAG AACAATGCAG AGAAGCCAGT GAAGTCAGTG 
CGGTTCCTGG CTCATGACAC TCCTCAGACA GCACCAAAAA CGCCGTCCAC TTCCACTGCA 
GGATTAAGCT CTAGTTCTCA GCCTGGCTTT TCAACTCTGG ACCACTGGAA TCCCTCGGCC 
ATACTGGGTC GAGATGGACA TACATTAGTC CTCAAAGACA CAAAAACCCC TCCAGCCAGA 
AAGAGCACAG GCCAAGATCA TGCTGCTATG GACTCCCTCG CGCTAAATAT GAAGCCAGTA 
CTGATCAACA ACCAATCCGG CTCCAGTAGA TGTAGGGTAA TTAAGGGATA CAACGCCAAA 
ACAGATGCAG AGCTCACGCT CACTGAAGGA GAAATGGTCC TGGTTCAGAG GCCTCGAGCA 
GACGGAAGAG TTCTGGTGAC GCAGGAGATC AGTGGAAAAA CAGGCCTTTT CCAAAGCAGC 
GTTCTGGAAC TGCTAGACAA AATCCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sh3rf2 ( XM_009291057.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009289332.1 Danio rerio SH3 domain containing ring finger 2 (sh3rf2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa19580
Clone ID Related Accession (Same CDS sequence)	XM_009291057.3 , XM_009291057.2 , XM_021474306.1 , XM_021481154.1
Accession Version	XM_009291057.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	putative E3 ubiquitin-protein ligase SH3RF2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291057.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGATC TGGCTGTTAT GGAGCTGTTA GAGTGTCCTT TGTGTATGGA GCCATTGGAC 
GTGTCTGCAA AGGTCCTGCC ATGCCAGCAC ACGTTCTGTA AATCATGCCT TCAGCAGCAG 
GAGACATCAC ACCCTGATCA GTTGTGCTGC CCAGAGTGCA GAGCTGCTGT TCCTGGATCA 
GTGGAGGAGC TTCCCACTAA CCCGCTGCTG CATAGACTCT TAGAGAGCCT TCACGAATGG 
GGTCCAGTGA GCACACCGAG GGACAGATCT GTCAGATATA TGAGCTCTCC GGCTCAGGAG 
GACCATTGCA GCAGTTACAC GCAAGATCTT CCCAAGCAGG AGCAGAACAC GCAGAGGGTT 
CAAGCCCAAG CATTATATGA CTTCCAGGGT AATGCTCCAG GGGAGCTCAC AATGAAGTCA 
GGTGACATCA TTTATCTGCG GTGGAGAGTA GATGATAACT GGTATTATGG GGAAGGCAAA 
GACAGCAGAG GTTTGGTTCC AGTCAATGTG TTGCAGGTCT TGAGTGAACA GCCTCAGCCT 
CTAGCCCTCT GCAGAGCTCT TTATGACTTC AATGCTAACA ATCTGGATCC AGAGGGCAGC 
AAGGAATGCT TAACCTTCTT CAAGGGAGAC AGCATTACTC TCCTTAAACA AGTCAATGAA 
AATTGGGTGG AAGGTAAACT CGGAGACAAA GTTGGGATTT TTCCTTTGCA ACTCACAGAG 
CCAAATCCGG CGGCATACGA GCTTCTGGAA AAGCATAAAA GGAGGGACTC AGTGGAAGCG 
CAGCCAAGGA GCAGCGTTAG GGTCAACGCA GATGCTTGCA TCCACAGGCG GCTCACTGGT 
CCATCTCAGA AGTCATCCAG GCCTTCAAAT GTCAATCTCT TGAACAGCCT AAACTGTCCT 
CCCCTCAGCC TGAGCCAGCA GCCTCCTCAC ATCAGCTCCA GCGCACAGCC ATCCTACAGC 
AAGACGCCAC AACTCGCCAC ATTTAACAGA CCGCGTCTGA GGAGCTCTCG CAGAAACTTG 
CCTAAGAGAC ACAGAACAAT GAACGGTGAA AGTCCGCCGG CCATCACCAT GGCACTGATT 
AACCCTCAGG CCTCGCCCAT GCCCTCAGAG AGCAAGATGT CTGCAACTCA GCAGCTCTCC 
ATCAGTGTGT GTGCGGCGCT GTATTCCTAC ACACCTTATC GCTCTGAAGA GCTGGAACTG 
AGGAAAGGTG AAATGGTGGG GGTTTATGGG AAATTCAGCG AGGGCTGGCT GCGCGGACTG 
TCTCTCAAAA CTGGCAAAGT AGGAATCCTG CCTACCAACT ATGTTACACC AGTGCTCAGA 
ACATCAGCAA GGTTTCTCGA ACAAAGCAAA CCTGCCATTC CTAATTCAAG TGCAGTGTCA 
ACAAAGCGCC ACGCCTCACA CAAGCCGCAG GCTGTTGTTC TGGCCCTCGA CAGGGTAAAC 
ACTGACAGCC CTTCAGCTAC AATGCCTGTG ACGTCATCTG CCGGTCCAGG AAGAGCAGCA 
CAGCTGGGCG GAAAGCAGGG CTGGAGCTCA GTGAGACGCT CCTTTCATGC CACACATAGG 
GGACTGCCTC ACCATGGAAG CTACAACTCA AACTCTATCC TAAGCCTGCA GCCTCCACCT 
CAGGACTTGG GGCAGATCTA CACTTTCGGT CGCTCACCTG TTCTTCCTAA AAAGAGAAAT 
GGTTTATTCT CCAACCCCAT GAAGGCACAG CATTGGGCTT ATGAAACAAC ATCTCCCTCT 
GCTGGTGCTT ATCAACCCAT CATCAACCCT GGCCAGAAGG AGACCACCAC AACTCGGCAG 
TCTATTCTGG TTAAACCAGA TGCTTACAAG AACAATGCAG AGAAGCCAGT GAAGTCAGTG 
CGGTTCCTGG CTCATGACAC TCCTCAGACA GCACCAAAAA CGCCGTCCAC TTCCACTGCA 
GGATTAAGCT CTAGTTCTCA GCCTGGCTTT TCAACTCTGG ACCACTGGAA TCCCTCGGCC 
ATACTGGGTC GAGATGGACA TACATTAGTC CTCAAAGACA CAAAAACCCC TCCAGCCAGA 
AAGAGCACAG GCCAAGATCA TGCTGCTATG GACTCCCTCG CGCTAAATAT GAAGCCAGTA 
CTGATCAACA ACCAATCCGG CTCCAGTAGA TGTAGGGTAA TTAAGGGATA CAACGCCAAA 
ACAGATGCAG AGCTCACGCT CACTGAAGGA GAAATGGTCC TGGTTCAGAG GCCTCGAGCA 
GACGGAAGAG TTCTGGTGAC GCAGGAGATC AGTGGAAAAA CAGGCCTTTT CCAAAGCAGC 
GTTCTGGAAC TGCTAGACAA AATCCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289332.1
CDS	110..2419
Translation	MEDLAVMELLECPLCMEPLDVSAKVLPCQHTFCKSCLQQQETSHPDQLCCPECRAAVPGSVEELPTNPLLHRLLESLHEWGPVSTPRDRSVRYMSSPAQEDHCSSYTQDLPKQEQNTQRVQAQALYDFQGNAPGELTMKSGDIIYLRWRVDDNWYYGEGKDSRGLVPVNVLQVLSEQPQPLALCRALYDFNANNLDPEGSKECLTFFKGDSITLLKQVNENWVEGKLGDKVGIFPLQLTEPNPAAYELLEKHKRRDSVEAQPRSSVRVNADACIHRRLTGPSQKSSRPSNVNLLNSLNCPPLSLSQQPPHISSSAQPSYSKTPQLATFNRPRLRSSRRNLPKRHRTMNGESPPAITMALINPQASPMPSESKMSATQQLSISVCAALYSYTPYRSEELELRKGEMVGVYGKFSEGWLRGLSLKTGKVGILPTNYVTPVLRTSARFLEQSKPAIPNSSAVSTKRHASHKPQAVVLALDRVNTDSPSATMPVTSSAGPGRAAQLGGKQGWSSVRRSFHATHRGLPHHGSYNSNSILSLQPPPQDLGQIYTFGRSPVLPKKRNGLFSNPMKAQHWAYETTSPSAGAYQPIINPGQKETTTTRQSILVKPDAYKNNAEKPVKSVRFLAHDTPQTAPKTPSTSTAGLSSSSQPGFSTLDHWNPSAILGRDGHTLVLKDTKTPPARKSTGQDHAAMDSLALNMKPVLINNQSGSSRCRVIKGYNAKTDAELTLTEGEMVLVQRPRADGRVLVTQEISGKTGLFQSSVLELLDKIL

Target ORF information:

RefSeq Version	XM_009291057.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio SH3 domain containing ring finger 2 (sh3rf2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291057.2

ORF Insert Sequence:

ATGGAGGATC TGGCTGTTAT GGAGCTGTTA GAGTGTCCTT TGTGTATGGA GCCATTGGAC 
GTGTCTGCAA AGGTCCTGCC ATGCCAGCAC ACGTTCTGTA AATCATGCCT TCAGCAGCAG 
GAGACATCAC ACCCTGATCA GTTGTGCTGC CCAGAGTGCA GAGCTGCTGT TCCTGGATCA 
GTGGAGGAGC TTCCCACTAA CCCGCTGCTG CATAGACTCT TAGAGAGCCT TCACGAATGG 
GGTCCAGTGA GCACACCGAG GGACAGATCT GTCAGATATA TGAGCTCTCC GGCTCAGGAG 
GACCATTGCA GCAGTTACAC GCAAGATCTT CCCAAGCAGG AGCAGAACAC GCAGAGGGTT 
CAAGCCCAAG CATTATATGA CTTCCAGGGT AATGCTCCAG GGGAGCTCAC AATGAAGTCA 
GGTGACATCA TTTATCTGCG GTGGAGAGTA GATGATAACT GGTATTATGG GGAAGGCAAA 
GACAGCAGAG GTTTGGTTCC AGTCAATGTG TTGCAGGTCT TGAGTGAACA GCCTCAGCCT 
CTAGCCCTCT GCAGAGCTCT TTATGACTTC AATGCTAACA ATCTGGATCC AGAGGGCAGC 
AAGGAATGCT TAACCTTCTT CAAGGGAGAC AGCATTACTC TCCTTAAACA AGTCAATGAA 
AATTGGGTGG AAGGTAAACT CGGAGACAAA GTTGGGATTT TTCCTTTGCA ACTCACAGAG 
CCAAATCCGG CGGCATACGA GCTTCTGGAA AAGCATAAAA GGAGGGACTC AGTGGAAGCG 
CAGCCAAGGA GCAGCGTTAG GGTCAACGCA GATGCTTGCA TCCACAGGCG GCTCACTGGT 
CCATCTCAGA AGTCATCCAG GCCTTCAAAT GTCAATCTCT TGAACAGCCT AAACTGTCCT 
CCCCTCAGCC TGAGCCAGCA GCCTCCTCAC ATCAGCTCCA GCGCACAGCC ATCCTACAGC 
AAGACGCCAC AACTCGCCAC ATTTAACAGA CCGCGTCTGA GGAGCTCTCG CAGAAACTTG 
CCTAAGAGAC ACAGAACAAT GAACGGTGAA AGTCCGCCGG CCATCACCAT GGCACTGATT 
AACCCTCAGG CCTCGCCCAT GCCCTCAGAG AGCAAGATGT CTGCAACTCA GCAGCTCTCC 
ATCAGTGTGT GTGCGGCGCT GTATTCCTAC ACACCTTATC GCTCTGAAGA GCTGGAACTG 
AGGAAAGGTG AAATGGTGGG GGTTTATGGG AAATTCAGCG AGGGCTGGCT GCGCGGACTG 
TCTCTCAAAA CTGGCAAAGT AGGAATCCTG CCTACCAACT ATGTTACACC AGTGCTCAGA 
ACATCAGCAA GGTTTCTCGA ACAAAGCAAA CCTGCCATTC CTAATTCAAG TGCAGTGTCA 
ACAAAGCGCC ACGCCTCACA CAAGCCGCAG GCTGTTGTTC TGGCCCTCGA CAGGGTAAAC 
ACTGACAGCC CTTCAGCTAC AATGCCTGTG ACGTCATCTG CCGGTCCAGG AAGAGCAGCA 
CAGCTGGGCG GAAAGCAGGG CTGGAGCTCA GTGAGACGCT CCTTTCATGC CACACATAGG 
GGACTGCCTC ACCATGGAAG CTACAACTCA AACTCTATCC TAAGCCTGCA GCCTCCACCT 
CAGGACTTGG GGCAGATCTA CACTTTCGGT CGCTCACCTG TTCTTCCTAA AAAGAGAAAT 
GGTTTATTCT CCAACCCCAT GAAGGCACAG CATTGGGCTT ATGAAACAAC ATCTCCCTCT 
GCTGGTGCTT ATCAACCCAT CATCAACCCT GGCCAGAAGG AGACCACCAC AACTCGGCAG 
TCTATTCTGG TTAAACCAGA TGCTTACAAG AACAATGCAG AGAAGCCAGT GAAGTCAGTG 
CGGTTCCTGG CTCATGACAC TCCTCAGACA GCACCAAAAA CGCCGTCCAC TTCCACTGCA 
GGATTAAGCT CTAGTTCTCA GCCTGGCTTT TCAACTCTGG ACCACTGGAA TCCCTCGGCC 
ATACTGGGTC GAGATGGACA TACATTAGTC CTCAAAGACA CAAAAACCCC TCCAGCCAGA 
AAGAGCACAG GCCAAGATCA TGCTGCTATG GACTCCCTCG CGCTAAATAT GAAGCCAGTA 
CTGATCAACA ACCAATCCGG CTCCAGTAGA TGTAGGGTAA TTAAGGGATA CAACGCCAAA 
ACAGATGCAG AGCTCACGCT CACTGAAGGA GAAATGGTCC TGGTTCAGAG GCCTCGAGCA 
GACGGAAGAG TTCTGGTGAC GCAGGAGATC AGTGGAAAAA CAGGCCTTTT CCAAAGCAGC 
GTTCTGGAAC TGCTAGACAA AATCCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sh3rf2 ( XM_021474306.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329981.1 Danio rerio SH3 domain containing ring finger 2 (sh3rf2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa19580
Clone ID Related Accession (Same CDS sequence)	XM_009291057.3 , XM_009291057.2 , XM_021474306.1 , XM_021481154.1
Accession Version	XM_021474306.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	putative E3 ubiquitin-protein ligase SH3RF2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395314.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGATC TGGCTGTTAT GGAGCTGTTA GAGTGTCCTT TGTGTATGGA GCCATTGGAC 
GTGTCTGCAA AGGTCCTGCC ATGCCAGCAC ACGTTCTGTA AATCATGCCT TCAGCAGCAG 
GAGACATCAC ACCCTGATCA GTTGTGCTGC CCAGAGTGCA GAGCTGCTGT TCCTGGATCA 
GTGGAGGAGC TTCCCACTAA CCCGCTGCTG CATAGACTCT TAGAGAGCCT TCACGAATGG 
GGTCCAGTGA GCACACCGAG GGACAGATCT GTCAGATATA TGAGCTCTCC GGCTCAGGAG 
GACCATTGCA GCAGTTACAC GCAAGATCTT CCCAAGCAGG AGCAGAACAC GCAGAGGGTT 
CAAGCCCAAG CATTATATGA CTTCCAGGGT AATGCTCCAG GGGAGCTCAC AATGAAGTCA 
GGTGACATCA TTTATCTGCG GTGGAGAGTA GATGATAACT GGTATTATGG GGAAGGCAAA 
GACAGCAGAG GTTTGGTTCC AGTCAATGTG TTGCAGGTCT TGAGTGAACA GCCTCAGCCT 
CTAGCCCTCT GCAGAGCTCT TTATGACTTC AATGCTAACA ATCTGGATCC AGAGGGCAGC 
AAGGAATGCT TAACCTTCTT CAAGGGAGAC AGCATTACTC TCCTTAAACA AGTCAATGAA 
AATTGGGTGG AAGGTAAACT CGGAGACAAA GTTGGGATTT TTCCTTTGCA ACTCACAGAG 
CCAAATCCGG CGGCATACGA GCTTCTGGAA AAGCATAAAA GGAGGGACTC AGTGGAAGCG 
CAGCCAAGGA GCAGCGTTAG GGTCAACGCA GATGCTTGCA TCCACAGGCG GCTCACTGGT 
CCATCTCAGA AGTCATCCAG GCCTTCAAAT GTCAATCTCT TGAACAGCCT AAACTGTCCT 
CCCCTCAGCC TGAGCCAGCA GCCTCCTCAC ATCAGCTCCA GCGCACAGCC ATCCTACAGC 
AAGACGCCAC AACTCGCCAC ATTTAACAGA CCGCGTCTGA GGAGCTCTCG CAGAAACTTG 
CCTAAGAGAC ACAGAACAAT GAACGGTGAA AGTCCGCCGG CCATCACCAT GGCACTGATT 
AACCCTCAGG CCTCGCCCAT GCCCTCAGAG AGCAAGATGT CTGCAACTCA GCAGCTCTCC 
ATCAGTGTGT GTGCGGCGCT GTATTCCTAC ACACCTTATC GCTCTGAAGA GCTGGAACTG 
AGGAAAGGTG AAATGGTGGG GGTTTATGGG AAATTCAGCG AGGGCTGGCT GCGCGGACTG 
TCTCTCAAAA CTGGCAAAGT AGGAATCCTG CCTACCAACT ATGTTACACC AGTGCTCAGA 
ACATCAGCAA GGTTTCTCGA ACAAAGCAAA CCTGCCATTC CTAATTCAAG TGCAGTGTCA 
ACAAAGCGCC ACGCCTCACA CAAGCCGCAG GCTGTTGTTC TGGCCCTCGA CAGGGTAAAC 
ACTGACAGCC CTTCAGCTAC AATGCCTGTG ACGTCATCTG CCGGTCCAGG AAGAGCAGCA 
CAGCTGGGCG GAAAGCAGGG CTGGAGCTCA GTGAGACGCT CCTTTCATGC CACACATAGG 
GGACTGCCTC ACCATGGAAG CTACAACTCA AACTCTATCC TAAGCCTGCA GCCTCCACCT 
CAGGACTTGG GGCAGATCTA CACTTTCGGT CGCTCACCTG TTCTTCCTAA AAAGAGAAAT 
GGTTTATTCT CCAACCCCAT GAAGGCACAG CATTGGGCTT ATGAAACAAC ATCTCCCTCT 
GCTGGTGCTT ATCAACCCAT CATCAACCCT GGCCAGAAGG AGACCACCAC AACTCGGCAG 
TCTATTCTGG TTAAACCAGA TGCTTACAAG AACAATGCAG AGAAGCCAGT GAAGTCAGTG 
CGGTTCCTGG CTCATGACAC TCCTCAGACA GCACCAAAAA CGCCGTCCAC TTCCACTGCA 
GGATTAAGCT CTAGTTCTCA GCCTGGCTTT TCAACTCTGG ACCACTGGAA TCCCTCGGCC 
ATACTGGGTC GAGATGGACA TACATTAGTC CTCAAAGACA CAAAAACCCC TCCAGCCAGA 
AAGAGCACAG GCCAAGATCA TGCTGCTATG GACTCCCTCG CGCTAAATAT GAAGCCAGTA 
CTGATCAACA ACCAATCCGG CTCCAGTAGA TGTAGGGTAA TTAAGGGATA CAACGCCAAA 
ACAGATGCAG AGCTCACGCT CACTGAAGGA GAAATGGTCC TGGTTCAGAG GCCTCGAGCA 
GACGGAAGAG TTCTGGTGAC GCAGGAGATC AGTGGAAAAA CAGGCCTTTT CCAAAGCAGC 
GTTCTGGAAC TGCTAGACAA AATCCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329981.1
CDS	144..2453
Translation	MEDLAVMELLECPLCMEPLDVSAKVLPCQHTFCKSCLQQQETSHPDQLCCPECRAAVPGSVEELPTNPLLHRLLESLHEWGPVSTPRDRSVRYMSSPAQEDHCSSYTQDLPKQEQNTQRVQAQALYDFQGNAPGELTMKSGDIIYLRWRVDDNWYYGEGKDSRGLVPVNVLQVLSEQPQPLALCRALYDFNANNLDPEGSKECLTFFKGDSITLLKQVNENWVEGKLGDKVGIFPLQLTEPNPAAYELLEKHKRRDSVEAQPRSSVRVNADACIHRRLTGPSQKSSRPSNVNLLNSLNCPPLSLSQQPPHISSSAQPSYSKTPQLATFNRPRLRSSRRNLPKRHRTMNGESPPAITMALINPQASPMPSESKMSATQQLSISVCAALYSYTPYRSEELELRKGEMVGVYGKFSEGWLRGLSLKTGKVGILPTNYVTPVLRTSARFLEQSKPAIPNSSAVSTKRHASHKPQAVVLALDRVNTDSPSATMPVTSSAGPGRAAQLGGKQGWSSVRRSFHATHRGLPHHGSYNSNSILSLQPPPQDLGQIYTFGRSPVLPKKRNGLFSNPMKAQHWAYETTSPSAGAYQPIINPGQKETTTTRQSILVKPDAYKNNAEKPVKSVRFLAHDTPQTAPKTPSTSTAGLSSSSQPGFSTLDHWNPSAILGRDGHTLVLKDTKTPPARKSTGQDHAAMDSLALNMKPVLINNQSGSSRCRVIKGYNAKTDAELTLTEGEMVLVQRPRADGRVLVTQEISGKTGLFQSSVLELLDKIL

Target ORF information:

RefSeq Version	XM_021474306.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SH3 domain containing ring finger 2 (sh3rf2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474306.1

ORF Insert Sequence:

ATGGAGGATC TGGCTGTTAT GGAGCTGTTA GAGTGTCCTT TGTGTATGGA GCCATTGGAC 
GTGTCTGCAA AGGTCCTGCC ATGCCAGCAC ACGTTCTGTA AATCATGCCT TCAGCAGCAG 
GAGACATCAC ACCCTGATCA GTTGTGCTGC CCAGAGTGCA GAGCTGCTGT TCCTGGATCA 
GTGGAGGAGC TTCCCACTAA CCCGCTGCTG CATAGACTCT TAGAGAGCCT TCACGAATGG 
GGTCCAGTGA GCACACCGAG GGACAGATCT GTCAGATATA TGAGCTCTCC GGCTCAGGAG 
GACCATTGCA GCAGTTACAC GCAAGATCTT CCCAAGCAGG AGCAGAACAC GCAGAGGGTT 
CAAGCCCAAG CATTATATGA CTTCCAGGGT AATGCTCCAG GGGAGCTCAC AATGAAGTCA 
GGTGACATCA TTTATCTGCG GTGGAGAGTA GATGATAACT GGTATTATGG GGAAGGCAAA 
GACAGCAGAG GTTTGGTTCC AGTCAATGTG TTGCAGGTCT TGAGTGAACA GCCTCAGCCT 
CTAGCCCTCT GCAGAGCTCT TTATGACTTC AATGCTAACA ATCTGGATCC AGAGGGCAGC 
AAGGAATGCT TAACCTTCTT CAAGGGAGAC AGCATTACTC TCCTTAAACA AGTCAATGAA 
AATTGGGTGG AAGGTAAACT CGGAGACAAA GTTGGGATTT TTCCTTTGCA ACTCACAGAG 
CCAAATCCGG CGGCATACGA GCTTCTGGAA AAGCATAAAA GGAGGGACTC AGTGGAAGCG 
CAGCCAAGGA GCAGCGTTAG GGTCAACGCA GATGCTTGCA TCCACAGGCG GCTCACTGGT 
CCATCTCAGA AGTCATCCAG GCCTTCAAAT GTCAATCTCT TGAACAGCCT AAACTGTCCT 
CCCCTCAGCC TGAGCCAGCA GCCTCCTCAC ATCAGCTCCA GCGCACAGCC ATCCTACAGC 
AAGACGCCAC AACTCGCCAC ATTTAACAGA CCGCGTCTGA GGAGCTCTCG CAGAAACTTG 
CCTAAGAGAC ACAGAACAAT GAACGGTGAA AGTCCGCCGG CCATCACCAT GGCACTGATT 
AACCCTCAGG CCTCGCCCAT GCCCTCAGAG AGCAAGATGT CTGCAACTCA GCAGCTCTCC 
ATCAGTGTGT GTGCGGCGCT GTATTCCTAC ACACCTTATC GCTCTGAAGA GCTGGAACTG 
AGGAAAGGTG AAATGGTGGG GGTTTATGGG AAATTCAGCG AGGGCTGGCT GCGCGGACTG 
TCTCTCAAAA CTGGCAAAGT AGGAATCCTG CCTACCAACT ATGTTACACC AGTGCTCAGA 
ACATCAGCAA GGTTTCTCGA ACAAAGCAAA CCTGCCATTC CTAATTCAAG TGCAGTGTCA 
ACAAAGCGCC ACGCCTCACA CAAGCCGCAG GCTGTTGTTC TGGCCCTCGA CAGGGTAAAC 
ACTGACAGCC CTTCAGCTAC AATGCCTGTG ACGTCATCTG CCGGTCCAGG AAGAGCAGCA 
CAGCTGGGCG GAAAGCAGGG CTGGAGCTCA GTGAGACGCT CCTTTCATGC CACACATAGG 
GGACTGCCTC ACCATGGAAG CTACAACTCA AACTCTATCC TAAGCCTGCA GCCTCCACCT 
CAGGACTTGG GGCAGATCTA CACTTTCGGT CGCTCACCTG TTCTTCCTAA AAAGAGAAAT 
GGTTTATTCT CCAACCCCAT GAAGGCACAG CATTGGGCTT ATGAAACAAC ATCTCCCTCT 
GCTGGTGCTT ATCAACCCAT CATCAACCCT GGCCAGAAGG AGACCACCAC AACTCGGCAG 
TCTATTCTGG TTAAACCAGA TGCTTACAAG AACAATGCAG AGAAGCCAGT GAAGTCAGTG 
CGGTTCCTGG CTCATGACAC TCCTCAGACA GCACCAAAAA CGCCGTCCAC TTCCACTGCA 
GGATTAAGCT CTAGTTCTCA GCCTGGCTTT TCAACTCTGG ACCACTGGAA TCCCTCGGCC 
ATACTGGGTC GAGATGGACA TACATTAGTC CTCAAAGACA CAAAAACCCC TCCAGCCAGA 
AAGAGCACAG GCCAAGATCA TGCTGCTATG GACTCCCTCG CGCTAAATAT GAAGCCAGTA 
CTGATCAACA ACCAATCCGG CTCCAGTAGA TGTAGGGTAA TTAAGGGATA CAACGCCAAA 
ACAGATGCAG AGCTCACGCT CACTGAAGGA GAAATGGTCC TGGTTCAGAG GCCTCGAGCA 
GACGGAAGAG TTCTGGTGAC GCAGGAGATC AGTGGAAAAA CAGGCCTTTT CCAAAGCAGC 
GTTCTGGAAC TGCTAGACAA AATCCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sh3rf2 ( XM_021481154.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021336829.1 Danio rerio SH3 domain containing ring finger 2 (sh3rf2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa19580
Clone ID Related Accession (Same CDS sequence)	XM_009291057.3 , XM_009291057.2 , XM_021474306.1 , XM_021481154.1
Accession Version	XM_021481154.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	putative E3 ubiquitin-protein ligase SH3RF2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGATC TGGCTGTTAT GGAGCTGTTA GAGTGTCCTT TGTGTATGGA GCCATTGGAC 
GTGTCTGCAA AGGTCCTGCC ATGCCAGCAC ACGTTCTGTA AATCATGCCT TCAGCAGCAG 
GAGACATCAC ACCCTGATCA GTTGTGCTGC CCAGAGTGCA GAGCTGCTGT TCCTGGATCA 
GTGGAGGAGC TTCCCACTAA CCCGCTGCTG CATAGACTCT TAGAGAGCCT TCACGAATGG 
GGTCCAGTGA GCACACCGAG GGACAGATCT GTCAGATATA TGAGCTCTCC GGCTCAGGAG 
GACCATTGCA GCAGTTACAC GCAAGATCTT CCCAAGCAGG AGCAGAACAC GCAGAGGGTT 
CAAGCCCAAG CATTATATGA CTTCCAGGGT AATGCTCCAG GGGAGCTCAC AATGAAGTCA 
GGTGACATCA TTTATCTGCG GTGGAGAGTA GATGATAACT GGTATTATGG GGAAGGCAAA 
GACAGCAGAG GTTTGGTTCC AGTCAATGTG TTGCAGGTCT TGAGTGAACA GCCTCAGCCT 
CTAGCCCTCT GCAGAGCTCT TTATGACTTC AATGCTAACA ATCTGGATCC AGAGGGCAGC 
AAGGAATGCT TAACCTTCTT CAAGGGAGAC AGCATTACTC TCCTTAAACA AGTCAATGAA 
AATTGGGTGG AAGGTAAACT CGGAGACAAA GTTGGGATTT TTCCTTTGCA ACTCACAGAG 
CCAAATCCGG CGGCATACGA GCTTCTGGAA AAGCATAAAA GGAGGGACTC AGTGGAAGCG 
CAGCCAAGGA GCAGCGTTAG GGTCAACGCA GATGCTTGCA TCCACAGGCG GCTCACTGGT 
CCATCTCAGA AGTCATCCAG GCCTTCAAAT GTCAATCTCT TGAACAGCCT AAACTGTCCT 
CCCCTCAGCC TGAGCCAGCA GCCTCCTCAC ATCAGCTCCA GCGCACAGCC ATCCTACAGC 
AAGACGCCAC AACTCGCCAC ATTTAACAGA CCGCGTCTGA GGAGCTCTCG CAGAAACTTG 
CCTAAGAGAC ACAGAACAAT GAACGGTGAA AGTCCGCCGG CCATCACCAT GGCACTGATT 
AACCCTCAGG CCTCGCCCAT GCCCTCAGAG AGCAAGATGT CTGCAACTCA GCAGCTCTCC 
ATCAGTGTGT GTGCGGCGCT GTATTCCTAC ACACCTTATC GCTCTGAAGA GCTGGAACTG 
AGGAAAGGTG AAATGGTGGG GGTTTATGGG AAATTCAGCG AGGGCTGGCT GCGCGGACTG 
TCTCTCAAAA CTGGCAAAGT AGGAATCCTG CCTACCAACT ATGTTACACC AGTGCTCAGA 
ACATCAGCAA GGTTTCTCGA ACAAAGCAAA CCTGCCATTC CTAATTCAAG TGCAGTGTCA 
ACAAAGCGCC ACGCCTCACA CAAGCCGCAG GCTGTTGTTC TGGCCCTCGA CAGGGTAAAC 
ACTGACAGCC CTTCAGCTAC AATGCCTGTG ACGTCATCTG CCGGTCCAGG AAGAGCAGCA 
CAGCTGGGCG GAAAGCAGGG CTGGAGCTCA GTGAGACGCT CCTTTCATGC CACACATAGG 
GGACTGCCTC ACCATGGAAG CTACAACTCA AACTCTATCC TAAGCCTGCA GCCTCCACCT 
CAGGACTTGG GGCAGATCTA CACTTTCGGT CGCTCACCTG TTCTTCCTAA AAAGAGAAAT 
GGTTTATTCT CCAACCCCAT GAAGGCACAG CATTGGGCTT ATGAAACAAC ATCTCCCTCT 
GCTGGTGCTT ATCAACCCAT CATCAACCCT GGCCAGAAGG AGACCACCAC AACTCGGCAG 
TCTATTCTGG TTAAACCAGA TGCTTACAAG AACAATGCAG AGAAGCCAGT GAAGTCAGTG 
CGGTTCCTGG CTCATGACAC TCCTCAGACA GCACCAAAAA CGCCGTCCAC TTCCACTGCA 
GGATTAAGCT CTAGTTCTCA GCCTGGCTTT TCAACTCTGG ACCACTGGAA TCCCTCGGCC 
ATACTGGGTC GAGATGGACA TACATTAGTC CTCAAAGACA CAAAAACCCC TCCAGCCAGA 
AAGAGCACAG GCCAAGATCA TGCTGCTATG GACTCCCTCG CGCTAAATAT GAAGCCAGTA 
CTGATCAACA ACCAATCCGG CTCCAGTAGA TGTAGGGTAA TTAAGGGATA CAACGCCAAA 
ACAGATGCAG AGCTCACGCT CACTGAAGGA GAAATGGTCC TGGTTCAGAG GCCTCGAGCA 
GACGGAAGAG TTCTGGTGAC GCAGGAGATC AGTGGAAAAA CAGGCCTTTT CCAAAGCAGC 
GTTCTGGAAC TGCTAGACAA AATCCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336829.1
CDS	144..2453
Translation	MEDLAVMELLECPLCMEPLDVSAKVLPCQHTFCKSCLQQQETSHPDQLCCPECRAAVPGSVEELPTNPLLHRLLESLHEWGPVSTPRDRSVRYMSSPAQEDHCSSYTQDLPKQEQNTQRVQAQALYDFQGNAPGELTMKSGDIIYLRWRVDDNWYYGEGKDSRGLVPVNVLQVLSEQPQPLALCRALYDFNANNLDPEGSKECLTFFKGDSITLLKQVNENWVEGKLGDKVGIFPLQLTEPNPAAYELLEKHKRRDSVEAQPRSSVRVNADACIHRRLTGPSQKSSRPSNVNLLNSLNCPPLSLSQQPPHISSSAQPSYSKTPQLATFNRPRLRSSRRNLPKRHRTMNGESPPAITMALINPQASPMPSESKMSATQQLSISVCAALYSYTPYRSEELELRKGEMVGVYGKFSEGWLRGLSLKTGKVGILPTNYVTPVLRTSARFLEQSKPAIPNSSAVSTKRHASHKPQAVVLALDRVNTDSPSATMPVTSSAGPGRAAQLGGKQGWSSVRRSFHATHRGLPHHGSYNSNSILSLQPPPQDLGQIYTFGRSPVLPKKRNGLFSNPMKAQHWAYETTSPSAGAYQPIINPGQKETTTTRQSILVKPDAYKNNAEKPVKSVRFLAHDTPQTAPKTPSTSTAGLSSSSQPGFSTLDHWNPSAILGRDGHTLVLKDTKTPPARKSTGQDHAAMDSLALNMKPVLINNQSGSSRCRVIKGYNAKTDAELTLTEGEMVLVQRPRADGRVLVTQEISGKTGLFQSSVLELLDKIL

Target ORF information:

RefSeq Version	XM_021481154.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SH3 domain containing ring finger 2 (sh3rf2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481154.1

ORF Insert Sequence:

ATGGAGGATC TGGCTGTTAT GGAGCTGTTA GAGTGTCCTT TGTGTATGGA GCCATTGGAC 
GTGTCTGCAA AGGTCCTGCC ATGCCAGCAC ACGTTCTGTA AATCATGCCT TCAGCAGCAG 
GAGACATCAC ACCCTGATCA GTTGTGCTGC CCAGAGTGCA GAGCTGCTGT TCCTGGATCA 
GTGGAGGAGC TTCCCACTAA CCCGCTGCTG CATAGACTCT TAGAGAGCCT TCACGAATGG 
GGTCCAGTGA GCACACCGAG GGACAGATCT GTCAGATATA TGAGCTCTCC GGCTCAGGAG 
GACCATTGCA GCAGTTACAC GCAAGATCTT CCCAAGCAGG AGCAGAACAC GCAGAGGGTT 
CAAGCCCAAG CATTATATGA CTTCCAGGGT AATGCTCCAG GGGAGCTCAC AATGAAGTCA 
GGTGACATCA TTTATCTGCG GTGGAGAGTA GATGATAACT GGTATTATGG GGAAGGCAAA 
GACAGCAGAG GTTTGGTTCC AGTCAATGTG TTGCAGGTCT TGAGTGAACA GCCTCAGCCT 
CTAGCCCTCT GCAGAGCTCT TTATGACTTC AATGCTAACA ATCTGGATCC AGAGGGCAGC 
AAGGAATGCT TAACCTTCTT CAAGGGAGAC AGCATTACTC TCCTTAAACA AGTCAATGAA 
AATTGGGTGG AAGGTAAACT CGGAGACAAA GTTGGGATTT TTCCTTTGCA ACTCACAGAG 
CCAAATCCGG CGGCATACGA GCTTCTGGAA AAGCATAAAA GGAGGGACTC AGTGGAAGCG 
CAGCCAAGGA GCAGCGTTAG GGTCAACGCA GATGCTTGCA TCCACAGGCG GCTCACTGGT 
CCATCTCAGA AGTCATCCAG GCCTTCAAAT GTCAATCTCT TGAACAGCCT AAACTGTCCT 
CCCCTCAGCC TGAGCCAGCA GCCTCCTCAC ATCAGCTCCA GCGCACAGCC ATCCTACAGC 
AAGACGCCAC AACTCGCCAC ATTTAACAGA CCGCGTCTGA GGAGCTCTCG CAGAAACTTG 
CCTAAGAGAC ACAGAACAAT GAACGGTGAA AGTCCGCCGG CCATCACCAT GGCACTGATT 
AACCCTCAGG CCTCGCCCAT GCCCTCAGAG AGCAAGATGT CTGCAACTCA GCAGCTCTCC 
ATCAGTGTGT GTGCGGCGCT GTATTCCTAC ACACCTTATC GCTCTGAAGA GCTGGAACTG 
AGGAAAGGTG AAATGGTGGG GGTTTATGGG AAATTCAGCG AGGGCTGGCT GCGCGGACTG 
TCTCTCAAAA CTGGCAAAGT AGGAATCCTG CCTACCAACT ATGTTACACC AGTGCTCAGA 
ACATCAGCAA GGTTTCTCGA ACAAAGCAAA CCTGCCATTC CTAATTCAAG TGCAGTGTCA 
ACAAAGCGCC ACGCCTCACA CAAGCCGCAG GCTGTTGTTC TGGCCCTCGA CAGGGTAAAC 
ACTGACAGCC CTTCAGCTAC AATGCCTGTG ACGTCATCTG CCGGTCCAGG AAGAGCAGCA 
CAGCTGGGCG GAAAGCAGGG CTGGAGCTCA GTGAGACGCT CCTTTCATGC CACACATAGG 
GGACTGCCTC ACCATGGAAG CTACAACTCA AACTCTATCC TAAGCCTGCA GCCTCCACCT 
CAGGACTTGG GGCAGATCTA CACTTTCGGT CGCTCACCTG TTCTTCCTAA AAAGAGAAAT 
GGTTTATTCT CCAACCCCAT GAAGGCACAG CATTGGGCTT ATGAAACAAC ATCTCCCTCT 
GCTGGTGCTT ATCAACCCAT CATCAACCCT GGCCAGAAGG AGACCACCAC AACTCGGCAG 
TCTATTCTGG TTAAACCAGA TGCTTACAAG AACAATGCAG AGAAGCCAGT GAAGTCAGTG 
CGGTTCCTGG CTCATGACAC TCCTCAGACA GCACCAAAAA CGCCGTCCAC TTCCACTGCA 
GGATTAAGCT CTAGTTCTCA GCCTGGCTTT TCAACTCTGG ACCACTGGAA TCCCTCGGCC 
ATACTGGGTC GAGATGGACA TACATTAGTC CTCAAAGACA CAAAAACCCC TCCAGCCAGA 
AAGAGCACAG GCCAAGATCA TGCTGCTATG GACTCCCTCG CGCTAAATAT GAAGCCAGTA 
CTGATCAACA ACCAATCCGG CTCCAGTAGA TGTAGGGTAA TTAAGGGATA CAACGCCAAA 
ACAGATGCAG AGCTCACGCT CACTGAAGGA GAAATGGTCC TGGTTCAGAG GCCTCGAGCA 
GACGGAAGAG TTCTGGTGAC GCAGGAGATC AGTGGAAAAA CAGGCCTTTT CCAAAGCAGC 
GTTCTGGAAC TGCTAGACAA AATCCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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sh3rf2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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電話

FAX

オンラインフォーム

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