buc2l cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	buc2l
Entrez Gene ID	100537720
Full Name	bucky ball 2-like
Synonyms	fi76b10,si:ch73-119o17.1,wu:fi76b10
General protein information	Preferred Names bucky ball 2-like Names uncharacterized protein buc2l
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003199579.4	XP_003199627.1	uncharacterized protein si:ch73-119o17.1
XM_003199579.5	XP_003199627.1	uncharacterized protein buc2l

Pathways from BioSystems
Gene Ontology

The following buc2l gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the buc2l cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa17706	XM_003199579.5 Latest version!	Danio rerio bucky ball 2-like (buc2l), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
View Old Accession Versions >>
ODa17706	XM_003199579.4	Danio rerio si:ch73-119o17.1 (si:ch73-119o17.1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

buc2l ( XM_003199579.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_003199627.1 Danio rerio si:ch73-119o17.1 (si:ch73-119o17.1), mRNA.

Price & Availability↑

CloneID	ODa17706
Clone ID Related Accession (Same CDS sequence)	XM_003199579.4 , XM_003199579.5
Accession Version	XM_003199579.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	uncharacterized protein si:ch73-119o17.1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_003199579.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTA TATCTACTCA TGAACCCATT GGTGGTTTGT CAGGCGTTCT TAGTGAACCA 
CTGCATGCAC AGGCCCCTCT ATGCGCTGGT CCTCTGCCCT CCAGAATACT ACACCCAGAT 
GAGCAGCTTC AGCTAAACCA GCAATTTTAT GTTCCCACTG TTCAGCCTTT CATGCCCTAT 
CAGTTTCCTA CATCATCTCT ATGTGTGCCA TACAGTGGAT TTCATGGCTT AGGGTATAGT 
GTGATGCCAC TACAACTTCC ATCATGTGTT GAAGTCCCTG GATTCATAAC ACCTCAACTG 
CAAATGTTGG ACTACAGACG TATAACCCCT CATGTGGCTC CAGCTACTGC CCACCGGACC 
CGCCACTCCA ATTTCCAAAA CCCTGTGCCA CTTGGTCCTG TGATGGTCAA CTCAGAGGTG 
CAAACTGAAC CTATTTGTCA TAGCATAGGC TCACATGGAC CTGCAGCGAG ATCAGACACC 
TGTTCAGAAT CAGGTCGTGG TACTGGCTGT GATAGTCCAG TGTCAACAAC ACCCAGGTCC 
ACTGAGAACA AATCCATAGC TTGCCCTGAA GGTGTGTCTG TGCTCACTCA AAATTGCAAC 
ACCAGTGCTA CTGAGACCAC TGATTTGTCT ACTGTACCCA AAAGTGAAAT CATCCAAGCT 
GAACAGGTGC AGAGCAAATG TGGTGTATTG CTGTCTAAGC ATGAGATTAT TCAGAACAAG 
AACAATAAGA TGGCAAGTTA TAACAAAAAT GACATTATGC AGTGCAGTTT GGGATCTGTG 
CAATCTTCAG AGGATGTTGT GGTGTGTTCT TACCGGTCAT TAGCATTCAG GGAGGATAAA 
CAGAGGGTTG AAGCTGGGAT GTTAAGTTAT TCCAGAAAGC ATCATCTAAC ATCTTGCACA 
GAACTTAATG TTTTGAGAAC TCCGTCTTGT ACCTTAAAAA AAATTTCTAA ACCAAGCAAA 
TCGGTCATTG CACAATGTGT TAAAGTAAAA AACAACTCCG AGACACAATT TCAGACAAAG 
GACAAGGATG ATGGAAATTC TCCTAATAAT AATTTTAAGA TTCTCCGACT GCCATTTGAT 
GTGCAGAACC ATAAACTGCC TTGTCAGCTT GATGCTTCTA TCTGGTCGGT AGAGTCTCTG 
TTACCATATG TGCCATCCAG TGAGTGGCTG GCAGAAAATG GTCTTCTAAC CCCTCAGAAA 
ACCTTAAGCT CACTGACTAA ACCCAGTAAT GTTATATCAA AGCCATACTG TGTTTCTGTG 
GGGAAAAATT TACAAAGTGG TCCATCAGCA AAATATTGTG TTTCTCCACA TCAGTTAGAG 
GCTTCGATCT GGTCCATAGA TTCTTTGATG CCATATGTGC CTTCAAATGA GTGGATGGTT 
GAGAATGGGT TTTCAACGCC TCAAAAAGGT GTGAGCCCAC AGATTAAATC CAGTGATGGT 
CATTCTTCCA CTGATGGCAT TCCTTTAAGG AAAAATCTGC AAGCATGTGG ATCAACTAAA 
TATCATGGTG TTTCCCATCA GTTAGAGGCT TCGATCTGGT CTGTAGATTC TTTGATCCCA 
TATGTGCCTC CCAATGAGTT GATGGTTGAT AATGGGTTTT CAACACCTCA AAAATCCGTT 
AGTCCACAGA TTAAATCCAG TGATGGTGAT TTTTCCACTG ATGGCATTCC TTTAAGGAAA 
AATCTGCAAA CATGTGGATC AACTAAATAT CACCGTGTTT CCCATCAGTT AGAAGCTTCA 
ATCTGGTCTG TAGATTCTTT GATGCCTTAT GTGCCTTCCA ATGAGTTGAT GGTTGATAAT 
GGGTTTTCAA CGCCTCAAAA AGGCATTAGC CCACAGATAA AATCCAGTGA TGGTTATTCT 
TCCACTGATG GCATTCCTTT AAAGAAAAAT CTGCAAACAT GTGGATCAGC AAAATATCAT 
GGTGTTTCCC ATCAGTTAGA GGCTTCTATC TGGTCAGTAG AATCTTTGAA GCCGTATGTA 
CCTTCTAATG GATATATGGT TGATAATGGG TATTTAACTT CTCATCAAGC TCTGAGCCCA 
CCGTTTAAAT CCAGTAATGT TGTGTCAGAG CCGAATCAGA CTTCCAGTGA TCATTTTCAA 
GTAGGGGAGA GGCTACAAAC TTGTGCATCT GCTAAACTTA AGGGGTCCAT TAAGTCACTC 
GAAAAACGCT CCCCTTACCG TCCCTCATCG AGCTGGCTGG CTGATTTTGG TAATGTATAC 
TACTACAGTA AGTTACCCCC TGTTCAGCAA CAGTCTGAGA TTCTGGAGAG ATGGCAGCCA 
AAAGTGTCCC TTACCACTCA TCTTGAACCA TCTTTCATTA ATTTAAAAAA GACCAGAAAC 
CAAACTCCAG CAACTGGTAT CTCAGACCAG AAGCACTGCG TATCTAATAA ATGCAAGTGC 
AAAGTCAAAA GAAGTCCAAG GCTTTCTTGT GTCACACTTG AAAGTAAATT ACCTTTGTGC 
CAATCATGTC GATATGACAC CAAAGGAAAA GATTCAAAAA AGCATTCAGA TTGTCCTGGC 
TGCAAAAAAG AAGACTATGT TGAAGTTAAG ACTTTTGACA AGATCTCCAA CGAGGGTGTC 
CTTGATGCCA AAAACATGGC ATTGGGAAAT CTTTTGCCAG AATGCTGTGG TGCCGCTCGG 
GTGGAATTAA GACAACAGAC TGCACTGACC AGCCCTGCTC AAGATAAATG TGGAGACCTT 
GAATGGAGAC TTTGGGAAAA GAAGAAAGAT TTTATGAACC AGAATAAGAG TCAGATATGG 
AGTCTACAGA AAGAAAATGC AGCAAAAGAA TCAATGCAGG CGTCTATTTC TGTAAGAAGG 
ATTGAGAACA AAGAAAGCAT GCTCACAAGA AATCGCACTA GAGGGGAACA TGTCACTGGC 
CGTGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003199627.1
CDS	123..3011
Translation	MAAISTHEPIGGLSGVLSEPLHAQAPLCAGPLPSRILHPDEQLQLNQQFYVPTVQPFMPYQFPTSSLCVPYSGFHGLGYSVMPLQLPSCVEVPGFITPQLQMLDYRRITPHVAPATAHRTRHSNFQNPVPLGPVMVNSEVQTEPICHSIGSHGPAARSDTCSESGRGTGCDSPVSTTPRSTENKSIACPEGVSVLTQNCNTSATETTDLSTVPKSEIIQAEQVQSKCGVLLSKHEIIQNKNNKMASYNKNDIMQCSLGSVQSSEDVVVCSYRSLAFREDKQRVEAGMLSYSRKHHLTSCTELNVLRTPSCTLKKISKPSKSVIAQCVKVKNNSETQFQTKDKDDGNSPNNNFKILRLPFDVQNHKLPCQLDASIWSVESLLPYVPSSEWLAENGLLTPQKTLSSLTKPSNVISKPYCVSVGKNLQSGPSAKYCVSPHQLEASIWSIDSLMPYVPSNEWMVENGFSTPQKGVSPQIKSSDGHSSTDGIPLRKNLQACGSTKYHGVSHQLEASIWSVDSLIPYVPPNELMVDNGFSTPQKSVSPQIKSSDGDFSTDGIPLRKNLQTCGSTKYHRVSHQLEASIWSVDSLMPYVPSNELMVDNGFSTPQKGISPQIKSSDGYSSTDGIPLKKNLQTCGSAKYHGVSHQLEASIWSVESLKPYVPSNGYMVDNGYLTSHQALSPPFKSSNVVSEPNQTSSDHFQVGERLQTCASAKLKGSIKSLEKRSPYRPSSSWLADFGNVYYYSKLPPVQQQSEILERWQPKVSLTTHLEPSFINLKKTRNQTPATGISDQKHCVSNKCKCKVKRSPRLSCVTLESKLPLCQSCRYDTKGKDSKKHSDCPGCKKEDYVEVKTFDKISNEGVLDAKNMALGNLLPECCGAARVELRQQTALTSPAQDKCGDLEWRLWEKKKDFMNQNKSQIWSLQKENAAKESMQASISVRRIENKESMLTRNRTRGEHVTGRE

Target ORF information:

RefSeq Version	XM_003199579.4
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch73-119o17.1 (si:ch73-119o17.1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003199579.4

ORF Insert Sequence:

ATGGCTGCTA TATCTACTCA TGAACCCATT GGTGGTTTGT CAGGCGTTCT TAGTGAACCA 
CTGCATGCAC AGGCCCCTCT ATGCGCTGGT CCTCTGCCCT CCAGAATACT ACACCCAGAT 
GAGCAGCTTC AGCTAAACCA GCAATTTTAT GTTCCCACTG TTCAGCCTTT CATGCCCTAT 
CAGTTTCCTA CATCATCTCT ATGTGTGCCA TACAGTGGAT TTCATGGCTT AGGGTATAGT 
GTGATGCCAC TACAACTTCC ATCATGTGTT GAAGTCCCTG GATTCATAAC ACCTCAACTG 
CAAATGTTGG ACTACAGACG TATAACCCCT CATGTGGCTC CAGCTACTGC CCACCGGACC 
CGCCACTCCA ATTTCCAAAA CCCTGTGCCA CTTGGTCCTG TGATGGTCAA CTCAGAGGTG 
CAAACTGAAC CTATTTGTCA TAGCATAGGC TCACATGGAC CTGCAGCGAG ATCAGACACC 
TGTTCAGAAT CAGGTCGTGG TACTGGCTGT GATAGTCCAG TGTCAACAAC ACCCAGGTCC 
ACTGAGAACA AATCCATAGC TTGCCCTGAA GGTGTGTCTG TGCTCACTCA AAATTGCAAC 
ACCAGTGCTA CTGAGACCAC TGATTTGTCT ACTGTACCCA AAAGTGAAAT CATCCAAGCT 
GAACAGGTGC AGAGCAAATG TGGTGTATTG CTGTCTAAGC ATGAGATTAT TCAGAACAAG 
AACAATAAGA TGGCAAGTTA TAACAAAAAT GACATTATGC AGTGCAGTTT GGGATCTGTG 
CAATCTTCAG AGGATGTTGT GGTGTGTTCT TACCGGTCAT TAGCATTCAG GGAGGATAAA 
CAGAGGGTTG AAGCTGGGAT GTTAAGTTAT TCCAGAAAGC ATCATCTAAC ATCTTGCACA 
GAACTTAATG TTTTGAGAAC TCCGTCTTGT ACCTTAAAAA AAATTTCTAA ACCAAGCAAA 
TCGGTCATTG CACAATGTGT TAAAGTAAAA AACAACTCCG AGACACAATT TCAGACAAAG 
GACAAGGATG ATGGAAATTC TCCTAATAAT AATTTTAAGA TTCTCCGACT GCCATTTGAT 
GTGCAGAACC ATAAACTGCC TTGTCAGCTT GATGCTTCTA TCTGGTCGGT AGAGTCTCTG 
TTACCATATG TGCCATCCAG TGAGTGGCTG GCAGAAAATG GTCTTCTAAC CCCTCAGAAA 
ACCTTAAGCT CACTGACTAA ACCCAGTAAT GTTATATCAA AGCCATACTG TGTTTCTGTG 
GGGAAAAATT TACAAAGTGG TCCATCAGCA AAATATTGTG TTTCTCCACA TCAGTTAGAG 
GCTTCGATCT GGTCCATAGA TTCTTTGATG CCATATGTGC CTTCAAATGA GTGGATGGTT 
GAGAATGGGT TTTCAACGCC TCAAAAAGGT GTGAGCCCAC AGATTAAATC CAGTGATGGT 
CATTCTTCCA CTGATGGCAT TCCTTTAAGG AAAAATCTGC AAGCATGTGG ATCAACTAAA 
TATCATGGTG TTTCCCATCA GTTAGAGGCT TCGATCTGGT CTGTAGATTC TTTGATCCCA 
TATGTGCCTC CCAATGAGTT GATGGTTGAT AATGGGTTTT CAACACCTCA AAAATCCGTT 
AGTCCACAGA TTAAATCCAG TGATGGTGAT TTTTCCACTG ATGGCATTCC TTTAAGGAAA 
AATCTGCAAA CATGTGGATC AACTAAATAT CACCGTGTTT CCCATCAGTT AGAAGCTTCA 
ATCTGGTCTG TAGATTCTTT GATGCCTTAT GTGCCTTCCA ATGAGTTGAT GGTTGATAAT 
GGGTTTTCAA CGCCTCAAAA AGGCATTAGC CCACAGATAA AATCCAGTGA TGGTTATTCT 
TCCACTGATG GCATTCCTTT AAAGAAAAAT CTGCAAACAT GTGGATCAGC AAAATATCAT 
GGTGTTTCCC ATCAGTTAGA GGCTTCTATC TGGTCAGTAG AATCTTTGAA GCCGTATGTA 
CCTTCTAATG GATATATGGT TGATAATGGG TATTTAACTT CTCATCAAGC TCTGAGCCCA 
CCGTTTAAAT CCAGTAATGT TGTGTCAGAG CCGAATCAGA CTTCCAGTGA TCATTTTCAA 
GTAGGGGAGA GGCTACAAAC TTGTGCATCT GCTAAACTTA AGGGGTCCAT TAAGTCACTC 
GAAAAACGCT CCCCTTACCG TCCCTCATCG AGCTGGCTGG CTGATTTTGG TAATGTATAC 
TACTACAGTA AGTTACCCCC TGTTCAGCAA CAGTCTGAGA TTCTGGAGAG ATGGCAGCCA 
AAAGTGTCCC TTACCACTCA TCTTGAACCA TCTTTCATTA ATTTAAAAAA GACCAGAAAC 
CAAACTCCAG CAACTGGTAT CTCAGACCAG AAGCACTGCG TATCTAATAA ATGCAAGTGC 
AAAGTCAAAA GAAGTCCAAG GCTTTCTTGT GTCACACTTG AAAGTAAATT ACCTTTGTGC 
CAATCATGTC GATATGACAC CAAAGGAAAA GATTCAAAAA AGCATTCAGA TTGTCCTGGC 
TGCAAAAAAG AAGACTATGT TGAAGTTAAG ACTTTTGACA AGATCTCCAA CGAGGGTGTC 
CTTGATGCCA AAAACATGGC ATTGGGAAAT CTTTTGCCAG AATGCTGTGG TGCCGCTCGG 
GTGGAATTAA GACAACAGAC TGCACTGACC AGCCCTGCTC AAGATAAATG TGGAGACCTT 
GAATGGAGAC TTTGGGAAAA GAAGAAAGAT TTTATGAACC AGAATAAGAG TCAGATATGG 
AGTCTACAGA AAGAAAATGC AGCAAAAGAA TCAATGCAGG CGTCTATTTC TGTAAGAAGG 
ATTGAGAACA AAGAAAGCAT GCTCACAAGA AATCGCACTA GAGGGGAACA TGTCACTGGC 
CGTGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

buc2l ( XM_003199579.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_003199627.1 Danio rerio bucky ball 2-like (buc2l), mRNA.

Price & Availability↑

CloneID	ODa17706
Clone ID Related Accession (Same CDS sequence)	XM_003199579.4 , XM_003199579.5
Accession Version	XM_003199579.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein buc2l
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_003199579.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTA TATCTACTCA TGAACCCATT GGTGGTTTGT CAGGCGTTCT TAGTGAACCA 
CTGCATGCAC AGGCCCCTCT ATGCGCTGGT CCTCTGCCCT CCAGAATACT ACACCCAGAT 
GAGCAGCTTC AGCTAAACCA GCAATTTTAT GTTCCCACTG TTCAGCCTTT CATGCCCTAT 
CAGTTTCCTA CATCATCTCT ATGTGTGCCA TACAGTGGAT TTCATGGCTT AGGGTATAGT 
GTGATGCCAC TACAACTTCC ATCATGTGTT GAAGTCCCTG GATTCATAAC ACCTCAACTG 
CAAATGTTGG ACTACAGACG TATAACCCCT CATGTGGCTC CAGCTACTGC CCACCGGACC 
CGCCACTCCA ATTTCCAAAA CCCTGTGCCA CTTGGTCCTG TGATGGTCAA CTCAGAGGTG 
CAAACTGAAC CTATTTGTCA TAGCATAGGC TCACATGGAC CTGCAGCGAG ATCAGACACC 
TGTTCAGAAT CAGGTCGTGG TACTGGCTGT GATAGTCCAG TGTCAACAAC ACCCAGGTCC 
ACTGAGAACA AATCCATAGC TTGCCCTGAA GGTGTGTCTG TGCTCACTCA AAATTGCAAC 
ACCAGTGCTA CTGAGACCAC TGATTTGTCT ACTGTACCCA AAAGTGAAAT CATCCAAGCT 
GAACAGGTGC AGAGCAAATG TGGTGTATTG CTGTCTAAGC ATGAGATTAT TCAGAACAAG 
AACAATAAGA TGGCAAGTTA TAACAAAAAT GACATTATGC AGTGCAGTTT GGGATCTGTG 
CAATCTTCAG AGGATGTTGT GGTGTGTTCT TACCGGTCAT TAGCATTCAG GGAGGATAAA 
CAGAGGGTTG AAGCTGGGAT GTTAAGTTAT TCCAGAAAGC ATCATCTAAC ATCTTGCACA 
GAACTTAATG TTTTGAGAAC TCCGTCTTGT ACCTTAAAAA AAATTTCTAA ACCAAGCAAA 
TCGGTCATTG CACAATGTGT TAAAGTAAAA AACAACTCCG AGACACAATT TCAGACAAAG 
GACAAGGATG ATGGAAATTC TCCTAATAAT AATTTTAAGA TTCTCCGACT GCCATTTGAT 
GTGCAGAACC ATAAACTGCC TTGTCAGCTT GATGCTTCTA TCTGGTCGGT AGAGTCTCTG 
TTACCATATG TGCCATCCAG TGAGTGGCTG GCAGAAAATG GTCTTCTAAC CCCTCAGAAA 
ACCTTAAGCT CACTGACTAA ACCCAGTAAT GTTATATCAA AGCCATACTG TGTTTCTGTG 
GGGAAAAATT TACAAAGTGG TCCATCAGCA AAATATTGTG TTTCTCCACA TCAGTTAGAG 
GCTTCGATCT GGTCCATAGA TTCTTTGATG CCATATGTGC CTTCAAATGA GTGGATGGTT 
GAGAATGGGT TTTCAACGCC TCAAAAAGGT GTGAGCCCAC AGATTAAATC CAGTGATGGT 
CATTCTTCCA CTGATGGCAT TCCTTTAAGG AAAAATCTGC AAGCATGTGG ATCAACTAAA 
TATCATGGTG TTTCCCATCA GTTAGAGGCT TCGATCTGGT CTGTAGATTC TTTGATCCCA 
TATGTGCCTC CCAATGAGTT GATGGTTGAT AATGGGTTTT CAACACCTCA AAAATCCGTT 
AGTCCACAGA TTAAATCCAG TGATGGTGAT TTTTCCACTG ATGGCATTCC TTTAAGGAAA 
AATCTGCAAA CATGTGGATC AACTAAATAT CACCGTGTTT CCCATCAGTT AGAAGCTTCA 
ATCTGGTCTG TAGATTCTTT GATGCCTTAT GTGCCTTCCA ATGAGTTGAT GGTTGATAAT 
GGGTTTTCAA CGCCTCAAAA AGGCATTAGC CCACAGATAA AATCCAGTGA TGGTTATTCT 
TCCACTGATG GCATTCCTTT AAAGAAAAAT CTGCAAACAT GTGGATCAGC AAAATATCAT 
GGTGTTTCCC ATCAGTTAGA GGCTTCTATC TGGTCAGTAG AATCTTTGAA GCCGTATGTA 
CCTTCTAATG GATATATGGT TGATAATGGG TATTTAACTT CTCATCAAGC TCTGAGCCCA 
CCGTTTAAAT CCAGTAATGT TGTGTCAGAG CCGAATCAGA CTTCCAGTGA TCATTTTCAA 
GTAGGGGAGA GGCTACAAAC TTGTGCATCT GCTAAACTTA AGGGGTCCAT TAAGTCACTC 
GAAAAACGCT CCCCTTACCG TCCCTCATCG AGCTGGCTGG CTGATTTTGG TAATGTATAC 
TACTACAGTA AGTTACCCCC TGTTCAGCAA CAGTCTGAGA TTCTGGAGAG ATGGCAGCCA 
AAAGTGTCCC TTACCACTCA TCTTGAACCA TCTTTCATTA ATTTAAAAAA GACCAGAAAC 
CAAACTCCAG CAACTGGTAT CTCAGACCAG AAGCACTGCG TATCTAATAA ATGCAAGTGC 
AAAGTCAAAA GAAGTCCAAG GCTTTCTTGT GTCACACTTG AAAGTAAATT ACCTTTGTGC 
CAATCATGTC GATATGACAC CAAAGGAAAA GATTCAAAAA AGCATTCAGA TTGTCCTGGC 
TGCAAAAAAG AAGACTATGT TGAAGTTAAG ACTTTTGACA AGATCTCCAA CGAGGGTGTC 
CTTGATGCCA AAAACATGGC ATTGGGAAAT CTTTTGCCAG AATGCTGTGG TGCCGCTCGG 
GTGGAATTAA GACAACAGAC TGCACTGACC AGCCCTGCTC AAGATAAATG TGGAGACCTT 
GAATGGAGAC TTTGGGAAAA GAAGAAAGAT TTTATGAACC AGAATAAGAG TCAGATATGG 
AGTCTACAGA AAGAAAATGC AGCAAAAGAA TCAATGCAGG CGTCTATTTC TGTAAGAAGG 
ATTGAGAACA AAGAAAGCAT GCTCACAAGA AATCGCACTA GAGGGGAACA TGTCACTGGC 
CGTGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003199627.1
CDS	128..3016
Translation	MAAISTHEPIGGLSGVLSEPLHAQAPLCAGPLPSRILHPDEQLQLNQQFYVPTVQPFMPYQFPTSSLCVPYSGFHGLGYSVMPLQLPSCVEVPGFITPQLQMLDYRRITPHVAPATAHRTRHSNFQNPVPLGPVMVNSEVQTEPICHSIGSHGPAARSDTCSESGRGTGCDSPVSTTPRSTENKSIACPEGVSVLTQNCNTSATETTDLSTVPKSEIIQAEQVQSKCGVLLSKHEIIQNKNNKMASYNKNDIMQCSLGSVQSSEDVVVCSYRSLAFREDKQRVEAGMLSYSRKHHLTSCTELNVLRTPSCTLKKISKPSKSVIAQCVKVKNNSETQFQTKDKDDGNSPNNNFKILRLPFDVQNHKLPCQLDASIWSVESLLPYVPSSEWLAENGLLTPQKTLSSLTKPSNVISKPYCVSVGKNLQSGPSAKYCVSPHQLEASIWSIDSLMPYVPSNEWMVENGFSTPQKGVSPQIKSSDGHSSTDGIPLRKNLQACGSTKYHGVSHQLEASIWSVDSLIPYVPPNELMVDNGFSTPQKSVSPQIKSSDGDFSTDGIPLRKNLQTCGSTKYHRVSHQLEASIWSVDSLMPYVPSNELMVDNGFSTPQKGISPQIKSSDGYSSTDGIPLKKNLQTCGSAKYHGVSHQLEASIWSVESLKPYVPSNGYMVDNGYLTSHQALSPPFKSSNVVSEPNQTSSDHFQVGERLQTCASAKLKGSIKSLEKRSPYRPSSSWLADFGNVYYYSKLPPVQQQSEILERWQPKVSLTTHLEPSFINLKKTRNQTPATGISDQKHCVSNKCKCKVKRSPRLSCVTLESKLPLCQSCRYDTKGKDSKKHSDCPGCKKEDYVEVKTFDKISNEGVLDAKNMALGNLLPECCGAARVELRQQTALTSPAQDKCGDLEWRLWEKKKDFMNQNKSQIWSLQKENAAKESMQASISVRRIENKESMLTRNRTRGEHVTGRE

Target ORF information:

RefSeq Version	XM_003199579.5
Organism	Danio rerio(zebrafish)
Definition	Danio rerio bucky ball 2-like (buc2l), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003199579.5

ORF Insert Sequence:

ATGGCTGCTA TATCTACTCA TGAACCCATT GGTGGTTTGT CAGGCGTTCT TAGTGAACCA 
CTGCATGCAC AGGCCCCTCT ATGCGCTGGT CCTCTGCCCT CCAGAATACT ACACCCAGAT 
GAGCAGCTTC AGCTAAACCA GCAATTTTAT GTTCCCACTG TTCAGCCTTT CATGCCCTAT 
CAGTTTCCTA CATCATCTCT ATGTGTGCCA TACAGTGGAT TTCATGGCTT AGGGTATAGT 
GTGATGCCAC TACAACTTCC ATCATGTGTT GAAGTCCCTG GATTCATAAC ACCTCAACTG 
CAAATGTTGG ACTACAGACG TATAACCCCT CATGTGGCTC CAGCTACTGC CCACCGGACC 
CGCCACTCCA ATTTCCAAAA CCCTGTGCCA CTTGGTCCTG TGATGGTCAA CTCAGAGGTG 
CAAACTGAAC CTATTTGTCA TAGCATAGGC TCACATGGAC CTGCAGCGAG ATCAGACACC 
TGTTCAGAAT CAGGTCGTGG TACTGGCTGT GATAGTCCAG TGTCAACAAC ACCCAGGTCC 
ACTGAGAACA AATCCATAGC TTGCCCTGAA GGTGTGTCTG TGCTCACTCA AAATTGCAAC 
ACCAGTGCTA CTGAGACCAC TGATTTGTCT ACTGTACCCA AAAGTGAAAT CATCCAAGCT 
GAACAGGTGC AGAGCAAATG TGGTGTATTG CTGTCTAAGC ATGAGATTAT TCAGAACAAG 
AACAATAAGA TGGCAAGTTA TAACAAAAAT GACATTATGC AGTGCAGTTT GGGATCTGTG 
CAATCTTCAG AGGATGTTGT GGTGTGTTCT TACCGGTCAT TAGCATTCAG GGAGGATAAA 
CAGAGGGTTG AAGCTGGGAT GTTAAGTTAT TCCAGAAAGC ATCATCTAAC ATCTTGCACA 
GAACTTAATG TTTTGAGAAC TCCGTCTTGT ACCTTAAAAA AAATTTCTAA ACCAAGCAAA 
TCGGTCATTG CACAATGTGT TAAAGTAAAA AACAACTCCG AGACACAATT TCAGACAAAG 
GACAAGGATG ATGGAAATTC TCCTAATAAT AATTTTAAGA TTCTCCGACT GCCATTTGAT 
GTGCAGAACC ATAAACTGCC TTGTCAGCTT GATGCTTCTA TCTGGTCGGT AGAGTCTCTG 
TTACCATATG TGCCATCCAG TGAGTGGCTG GCAGAAAATG GTCTTCTAAC CCCTCAGAAA 
ACCTTAAGCT CACTGACTAA ACCCAGTAAT GTTATATCAA AGCCATACTG TGTTTCTGTG 
GGGAAAAATT TACAAAGTGG TCCATCAGCA AAATATTGTG TTTCTCCACA TCAGTTAGAG 
GCTTCGATCT GGTCCATAGA TTCTTTGATG CCATATGTGC CTTCAAATGA GTGGATGGTT 
GAGAATGGGT TTTCAACGCC TCAAAAAGGT GTGAGCCCAC AGATTAAATC CAGTGATGGT 
CATTCTTCCA CTGATGGCAT TCCTTTAAGG AAAAATCTGC AAGCATGTGG ATCAACTAAA 
TATCATGGTG TTTCCCATCA GTTAGAGGCT TCGATCTGGT CTGTAGATTC TTTGATCCCA 
TATGTGCCTC CCAATGAGTT GATGGTTGAT AATGGGTTTT CAACACCTCA AAAATCCGTT 
AGTCCACAGA TTAAATCCAG TGATGGTGAT TTTTCCACTG ATGGCATTCC TTTAAGGAAA 
AATCTGCAAA CATGTGGATC AACTAAATAT CACCGTGTTT CCCATCAGTT AGAAGCTTCA 
ATCTGGTCTG TAGATTCTTT GATGCCTTAT GTGCCTTCCA ATGAGTTGAT GGTTGATAAT 
GGGTTTTCAA CGCCTCAAAA AGGCATTAGC CCACAGATAA AATCCAGTGA TGGTTATTCT 
TCCACTGATG GCATTCCTTT AAAGAAAAAT CTGCAAACAT GTGGATCAGC AAAATATCAT 
GGTGTTTCCC ATCAGTTAGA GGCTTCTATC TGGTCAGTAG AATCTTTGAA GCCGTATGTA 
CCTTCTAATG GATATATGGT TGATAATGGG TATTTAACTT CTCATCAAGC TCTGAGCCCA 
CCGTTTAAAT CCAGTAATGT TGTGTCAGAG CCGAATCAGA CTTCCAGTGA TCATTTTCAA 
GTAGGGGAGA GGCTACAAAC TTGTGCATCT GCTAAACTTA AGGGGTCCAT TAAGTCACTC 
GAAAAACGCT CCCCTTACCG TCCCTCATCG AGCTGGCTGG CTGATTTTGG TAATGTATAC 
TACTACAGTA AGTTACCCCC TGTTCAGCAA CAGTCTGAGA TTCTGGAGAG ATGGCAGCCA 
AAAGTGTCCC TTACCACTCA TCTTGAACCA TCTTTCATTA ATTTAAAAAA GACCAGAAAC 
CAAACTCCAG CAACTGGTAT CTCAGACCAG AAGCACTGCG TATCTAATAA ATGCAAGTGC 
AAAGTCAAAA GAAGTCCAAG GCTTTCTTGT GTCACACTTG AAAGTAAATT ACCTTTGTGC 
CAATCATGTC GATATGACAC CAAAGGAAAA GATTCAAAAA AGCATTCAGA TTGTCCTGGC 
TGCAAAAAAG AAGACTATGT TGAAGTTAAG ACTTTTGACA AGATCTCCAA CGAGGGTGTC 
CTTGATGCCA AAAACATGGC ATTGGGAAAT CTTTTGCCAG AATGCTGTGG TGCCGCTCGG 
GTGGAATTAA GACAACAGAC TGCACTGACC AGCCCTGCTC AAGATAAATG TGGAGACCTT 
GAATGGAGAC TTTGGGAAAA GAAGAAAGAT TTTATGAACC AGAATAAGAG TCAGATATGG 
AGTCTACAGA AAGAAAATGC AGCAAAAGAA TCAATGCAGG CGTCTATTTC TGTAAGAAGG 
ATTGAGAACA AAGAAAGCAT GCTCACAAGA AATCGCACTA GAGGGGAACA TGTCACTGGC 
CGTGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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buc2l cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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