ptchd3a cDNA ORF clone, Danio rerio(zebrafish)

The following ptchd3a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ptchd3a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa28776 XM_003201327.5
Latest version!
Danio rerio patched domain containing 3a (ptchd3a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
ODa57750 XM_017354003.2
Latest version!
Danio rerio patched domain containing 3a (ptchd3a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
View Old Accession Versions >>
ODa28776 XM_003201327.4 Danio rerio patched domain containing 3a (ptchd3a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
ODa57750 XM_017354003.1 Danio rerio patched domain containing 3a (ptchd3a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa28776
    Clone ID Related Accession (Same CDS sequence) XM_003201327.5 , XM_003201327.4
    Accession Version XM_003201327.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2943bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product patched domain-containing protein 3-like isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_003201327.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAGCAAA ATGAGAAGAA ACCACAACGT AAACACTGCG TGGAGAAGCC GATCTCCATC 
    TGCTTCCAGA AGTTCGGCCG TTTTGTGGGC ACTTACCCCT GGTGGTTCTT CATCTCTCCC
    CTGCTCATCT CTGCTGTGTT AGGCACTGGC TTTTGCCTTC TAGAAGATCG TGAAGCCAAT
    GACATTGAGG ATCAGTTCAC ACCTGTAAAC GGTCCTGCCA AGCAGGAGAG GCGCTTTGTG
    CAGCAGAACT TTCCGCAGAA CGACTCGCTG TTCTCCAACC AAAGGCTCTA CACGGACGGG
    GTTTACGCGT CCTTCATAGC CGTGACTAAA TCCTCCAATA TCCTCACAGA CGCTGCTTTT
    CGGGAGATAG TGATTTTAGA CAGGAAAGTC AAGGAGCTGA ATGTGTCCAC GGGTCACGAG
    GTGCTTACTT TTGAGGGACT TTGCGCAAGA CGATACAAGA AGTGCATACC CAATAAGATA
    TTGGACATCC ATAAGTATTA CGGAGAGAAT CTGGAGGCCA CCGAGCTCTC CTTTCCGTTC
    TTCCGCTTCG GATTCTCCTA CATTTTCTTG GGTTATTCGG TCGGTGGAGT GAATTTGAAC
    TCTTCCATTA TTAAAAGCGC AAAGTCAGTG CGTCTGTTTT ATTTTCTAAA GGAGGATAAT
    CGTACTAGAA CTGATTTATG GCTGAATGAG TTCCTCAAGG TTTTCCCGTC TAATTTATCG
    CTAAACTCCA TAAAGGTGAC CCACTCCACA TCTTTGTCAA GGCAGGTGGA GTTTGAGGCA
    AACACAAAAG ATGTCATTCC TCTCTTCTCC ATCACTTACG TCATCGCTAT TGCTTTCTCC
    ATTCTTTCTT GCTTGAGGTT CGACTGTGTC AGAAACAAAG TGTGGGTTGC AACATTTGGG
    GTTTTCTCTG CTGGACTGGC TGTGTTGTCT TCTTTCGGCA TGATGTTACA CATTGGAGTC
    CCTTTTGTCA TGACAGTTGC TAATTCTCCA TTCCTAATAT TAGGGATTGG AGTAGACGAC
    ATGTTCATCC TCATCTCCTG TTGGCAGCAG ACGAATGTTC ACGATCGAGT GGAGACTCGC
    CTGTCTAATA CATACAAAGA GGCGGCCATT TCAATCACCA TCACCACTCT CACAGACGTA
    CTCGCTTTCT ACATCGGCCT CATGACGCCT TTCCGCTCCG TCCGCTCTTT TTGCCTGTAC
    ACCAGCACCT CCATCCTTTT CTGCTACATC TACAGCATCA CTTTTTTTGG AGCGTTCCTC
    GTCCTTAACG GACGGCGGGA GAACAGCAAC AAGCACTGGC TGACCTGCAA AGAAGTACCC
    GAAGAGTGTG CTGTAGGACA GTCAAAATGG TACGAATTAT GTTGCATTGG AGGAGCTTAC
    GACCGCCATA CTGGCTCAGA GGAGGTCCAA CCCATGAATC ACTTCTTCAA AAAATACTAC
    GGCCCATTTC TGACCAAATC CTGGACGAAG GTGTTTGTGA TCGTCTTTTA TTGCATCTAT
    TTAATCGTCA GCATTTACGG CTGCTTTCAG ATTCAGGAAG GAATCGATCT CCGCAATTTG
    GCGGCTGACG ATTCCTACGT GGTAAAATAC TACGATAACG AGAAGGCGTA TTTTTCCGAA
    TACGGCCCAA ACATCATGGT TGTCATCCGA CAGGAATTTC CATACTGGGA TGAAAAGTAC
    ATGTCGGATC TAGAAACCTG CATCGAAGAC TTCAAAAACT TATCCTTCAT CGAAAAGGAC
    ATATTCACGT CCTGGATTAA ATCCTACAAG TACTACGGAT ATCACACGAA GCTCAATCTG
    AGCGCAGAAG ACGTCTTCAA AGAGAATTTG GGAACGTTTC TACGGTTTTA CTCTGATTTC
    AAACAGGACG TTAACTTCAC CAACAACTCC ATCCACGCGT CCCGGTTTTT CATACAGACC
    GTAAACATCT CCACTGCCCT GGATGAAATG AACATGTTAA ATAAACTACG AGACACTGCC
    CAAAAGTGCC CTGTTCCCTT ATTGGTGTAC CACCCTGCCT TCATTTACCA CGACCAGTAT
    GCGGTCATAG TCACCAACAC CATCCAAAAC ATTGCGGTTA CCACAGCCGT GATGCTTCTG
    ATCTCCCTTC TCCTCATCCC GAATCCTCTG TGCTCCCTTT GGGTCACTTT CTCCATTGCA
    TCCGTCATCG TCGGAGTCAC TGGGTTCATG GCGTTATGGG ATGTCAACTT GGACACCATT
    TCCATGATCA TTCTTGTCGT CTGCATTGGG TTCTCCGTAG ACTTTTCTGC TCATATTTCA
    TATGCCTTTG TATCCAACAA GAAGCCCAGT GCGAACGAAA AAGCAGTGGA GGCTTTGTTT
    AATTTAGGAT ACCCCATTTT GCAGGGTGCT GTGTCCACTA TTCTTGGGGT GGTTGTCTTG
    TCTGCGTCCA AGAATTACAT TTTTAGGACT TTCTTTAAAA TTATGTTCTT GGTCATCTTC
    TTTGGGCTCT TCCATGGTTT GACTTTCATC CCTGTTTTCT TGACCTTCTT TGATATGTGT
    AACGGAACGC CGGCTGAAAA CAAATCAGGC CCGGAAGAGC AAGCCATGAA GAAGAGTTAC
    ACCAATAACA TTGCTAAGGA TGGTGACGTG AAAGAGCGGG AAATATATGA CAATCACACT
    TTCCAATCTG ACAACCATCA AACATGTCCG TCTCAGCCCT GCTCTGATAT ATGGATGATA
    GCCGGAAATA ATCATAGTGT GCAGGATAAT CACGTGTGCT TGGCGAGCAC TGTCCCAATG
    TTCAGAGTTG ACGGTCAGAC CTATGAGGTT CACATGTATG AACCGTCCGA TGATGTCATG
    TTAAAAGTCA ACTGTCAATC AAAACAGCGT GTCGAAGGAC AAAATTGTGT TTCGGGAAAC
    AGCAACCACA TTCAAGATTC AGGTCTTCCT ACTCCTAACT GTTCCGAGTT GACCATTTTG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003201375.2
    CDS264..3206
    Translation

    Target ORF information:

    RefSeq Version XM_003201327.5
    Organism Danio rerio(zebrafish)
    Definition Danio rerio patched domain containing 3a (ptchd3a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003201327.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAGCAAA ATGAGAAGAA ACCACAACGT AAACACTGCG TGGAGAAGCC GATCTCCATC 
    TGCTTCCAGA AGTTCGGCCG TTTTGTGGGC ACTTACCCCT GGTGGTTCTT CATCTCTCCC
    CTGCTCATCT CTGCTGTGTT AGGCACTGGC TTTTGCCTTC TAGAAGATCG TGAAGCCAAT
    GACATTGAGG ATCAGTTCAC ACCTGTAAAC GGTCCTGCCA AGCAGGAGAG GCGCTTTGTG
    CAGCAGAACT TTCCGCAGAA CGACTCGCTG TTCTCCAACC AAAGGCTCTA CACGGACGGG
    GTTTACGCGT CCTTCATAGC CGTGACTAAA TCCTCCAATA TCCTCACAGA CGCTGCTTTT
    CGGGAGATAG TGATTTTAGA CAGGAAAGTC AAGGAGCTGA ATGTGTCCAC GGGTCACGAG
    GTGCTTACTT TTGAGGGACT TTGCGCAAGA CGATACAAGA AGTGCATACC CAATAAGATA
    TTGGACATCC ATAAGTATTA CGGAGAGAAT CTGGAGGCCA CCGAGCTCTC CTTTCCGTTC
    TTCCGCTTCG GATTCTCCTA CATTTTCTTG GGTTATTCGG TCGGTGGAGT GAATTTGAAC
    TCTTCCATTA TTAAAAGCGC AAAGTCAGTG CGTCTGTTTT ATTTTCTAAA GGAGGATAAT
    CGTACTAGAA CTGATTTATG GCTGAATGAG TTCCTCAAGG TTTTCCCGTC TAATTTATCG
    CTAAACTCCA TAAAGGTGAC CCACTCCACA TCTTTGTCAA GGCAGGTGGA GTTTGAGGCA
    AACACAAAAG ATGTCATTCC TCTCTTCTCC ATCACTTACG TCATCGCTAT TGCTTTCTCC
    ATTCTTTCTT GCTTGAGGTT CGACTGTGTC AGAAACAAAG TGTGGGTTGC AACATTTGGG
    GTTTTCTCTG CTGGACTGGC TGTGTTGTCT TCTTTCGGCA TGATGTTACA CATTGGAGTC
    CCTTTTGTCA TGACAGTTGC TAATTCTCCA TTCCTAATAT TAGGGATTGG AGTAGACGAC
    ATGTTCATCC TCATCTCCTG TTGGCAGCAG ACGAATGTTC ACGATCGAGT GGAGACTCGC
    CTGTCTAATA CATACAAAGA GGCGGCCATT TCAATCACCA TCACCACTCT CACAGACGTA
    CTCGCTTTCT ACATCGGCCT CATGACGCCT TTCCGCTCCG TCCGCTCTTT TTGCCTGTAC
    ACCAGCACCT CCATCCTTTT CTGCTACATC TACAGCATCA CTTTTTTTGG AGCGTTCCTC
    GTCCTTAACG GACGGCGGGA GAACAGCAAC AAGCACTGGC TGACCTGCAA AGAAGTACCC
    GAAGAGTGTG CTGTAGGACA GTCAAAATGG TACGAATTAT GTTGCATTGG AGGAGCTTAC
    GACCGCCATA CTGGCTCAGA GGAGGTCCAA CCCATGAATC ACTTCTTCAA AAAATACTAC
    GGCCCATTTC TGACCAAATC CTGGACGAAG GTGTTTGTGA TCGTCTTTTA TTGCATCTAT
    TTAATCGTCA GCATTTACGG CTGCTTTCAG ATTCAGGAAG GAATCGATCT CCGCAATTTG
    GCGGCTGACG ATTCCTACGT GGTAAAATAC TACGATAACG AGAAGGCGTA TTTTTCCGAA
    TACGGCCCAA ACATCATGGT TGTCATCCGA CAGGAATTTC CATACTGGGA TGAAAAGTAC
    ATGTCGGATC TAGAAACCTG CATCGAAGAC TTCAAAAACT TATCCTTCAT CGAAAAGGAC
    ATATTCACGT CCTGGATTAA ATCCTACAAG TACTACGGAT ATCACACGAA GCTCAATCTG
    AGCGCAGAAG ACGTCTTCAA AGAGAATTTG GGAACGTTTC TACGGTTTTA CTCTGATTTC
    AAACAGGACG TTAACTTCAC CAACAACTCC ATCCACGCGT CCCGGTTTTT CATACAGACC
    GTAAACATCT CCACTGCCCT GGATGAAATG AACATGTTAA ATAAACTACG AGACACTGCC
    CAAAAGTGCC CTGTTCCCTT ATTGGTGTAC CACCCTGCCT TCATTTACCA CGACCAGTAT
    GCGGTCATAG TCACCAACAC CATCCAAAAC ATTGCGGTTA CCACAGCCGT GATGCTTCTG
    ATCTCCCTTC TCCTCATCCC GAATCCTCTG TGCTCCCTTT GGGTCACTTT CTCCATTGCA
    TCCGTCATCG TCGGAGTCAC TGGGTTCATG GCGTTATGGG ATGTCAACTT GGACACCATT
    TCCATGATCA TTCTTGTCGT CTGCATTGGG TTCTCCGTAG ACTTTTCTGC TCATATTTCA
    TATGCCTTTG TATCCAACAA GAAGCCCAGT GCGAACGAAA AAGCAGTGGA GGCTTTGTTT
    AATTTAGGAT ACCCCATTTT GCAGGGTGCT GTGTCCACTA TTCTTGGGGT GGTTGTCTTG
    TCTGCGTCCA AGAATTACAT TTTTAGGACT TTCTTTAAAA TTATGTTCTT GGTCATCTTC
    TTTGGGCTCT TCCATGGTTT GACTTTCATC CCTGTTTTCT TGACCTTCTT TGATATGTGT
    AACGGAACGC CGGCTGAAAA CAAATCAGGC CCGGAAGAGC AAGCCATGAA GAAGAGTTAC
    ACCAATAACA TTGCTAAGGA TGGTGACGTG AAAGAGCGGG AAATATATGA CAATCACACT
    TTCCAATCTG ACAACCATCA AACATGTCCG TCTCAGCCCT GCTCTGATAT ATGGATGATA
    GCCGGAAATA ATCATAGTGT GCAGGATAAT CACGTGTGCT TGGCGAGCAC TGTCCCAATG
    TTCAGAGTTG ACGGTCAGAC CTATGAGGTT CACATGTATG AACCGTCCGA TGATGTCATG
    TTAAAAGTCA ACTGTCAATC AAAACAGCGT GTCGAAGGAC AAAATTGTGT TTCGGGAAAC
    AGCAACCACA TTCAAGATTC AGGTCTTCCT ACTCCTAACT GTTCCGAGTT GACCATTTTG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa28776
    Clone ID Related Accession (Same CDS sequence) XM_003201327.5 , XM_003201327.4
    Accession Version XM_003201327.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2943bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product patched domain-containing protein 3-like isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_003201327.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAGCAAA ATGAGAAGAA ACCACAACGT AAACACTGCG TGGAGAAGCC GATCTCCATC 
    TGCTTCCAGA AGTTCGGCCG TTTTGTGGGC ACTTACCCCT GGTGGTTCTT CATCTCTCCC
    CTGCTCATCT CTGCTGTGTT AGGCACTGGC TTTTGCCTTC TAGAAGATCG TGAAGCCAAT
    GACATTGAGG ATCAGTTCAC ACCTGTAAAC GGTCCTGCCA AGCAGGAGAG GCGCTTTGTG
    CAGCAGAACT TTCCGCAGAA CGACTCGCTG TTCTCCAACC AAAGGCTCTA CACGGACGGG
    GTTTACGCGT CCTTCATAGC CGTGACTAAA TCCTCCAATA TCCTCACAGA CGCTGCTTTT
    CGGGAGATAG TGATTTTAGA CAGGAAAGTC AAGGAGCTGA ATGTGTCCAC GGGTCACGAG
    GTGCTTACTT TTGAGGGACT TTGCGCAAGA CGATACAAGA AGTGCATACC CAATAAGATA
    TTGGACATCC ATAAGTATTA CGGAGAGAAT CTGGAGGCCA CCGAGCTCTC CTTTCCGTTC
    TTCCGCTTCG GATTCTCCTA CATTTTCTTG GGTTATTCGG TCGGTGGAGT GAATTTGAAC
    TCTTCCATTA TTAAAAGCGC AAAGTCAGTG CGTCTGTTTT ATTTTCTAAA GGAGGATAAT
    CGTACTAGAA CTGATTTATG GCTGAATGAG TTCCTCAAGG TTTTCCCGTC TAATTTATCG
    CTAAACTCCA TAAAGGTGAC CCACTCCACA TCTTTGTCAA GGCAGGTGGA GTTTGAGGCA
    AACACAAAAG ATGTCATTCC TCTCTTCTCC ATCACTTACG TCATCGCTAT TGCTTTCTCC
    ATTCTTTCTT GCTTGAGGTT CGACTGTGTC AGAAACAAAG TGTGGGTTGC AACATTTGGG
    GTTTTCTCTG CTGGACTGGC TGTGTTGTCT TCTTTCGGCA TGATGTTACA CATTGGAGTC
    CCTTTTGTCA TGACAGTTGC TAATTCTCCA TTCCTAATAT TAGGGATTGG AGTAGACGAC
    ATGTTCATCC TCATCTCCTG TTGGCAGCAG ACGAATGTTC ACGATCGAGT GGAGACTCGC
    CTGTCTAATA CATACAAAGA GGCGGCCATT TCAATCACCA TCACCACTCT CACAGACGTA
    CTCGCTTTCT ACATCGGCCT CATGACGCCT TTCCGCTCCG TCCGCTCTTT TTGCCTGTAC
    ACCAGCACCT CCATCCTTTT CTGCTACATC TACAGCATCA CTTTTTTTGG AGCGTTCCTC
    GTCCTTAACG GACGGCGGGA GAACAGCAAC AAGCACTGGC TGACCTGCAA AGAAGTACCC
    GAAGAGTGTG CTGTAGGACA GTCAAAATGG TACGAATTAT GTTGCATTGG AGGAGCTTAC
    GACCGCCATA CTGGCTCAGA GGAGGTCCAA CCCATGAATC ACTTCTTCAA AAAATACTAC
    GGCCCATTTC TGACCAAATC CTGGACGAAG GTGTTTGTGA TCGTCTTTTA TTGCATCTAT
    TTAATCGTCA GCATTTACGG CTGCTTTCAG ATTCAGGAAG GAATCGATCT CCGCAATTTG
    GCGGCTGACG ATTCCTACGT GGTAAAATAC TACGATAACG AGAAGGCGTA TTTTTCCGAA
    TACGGCCCAA ACATCATGGT TGTCATCCGA CAGGAATTTC CATACTGGGA TGAAAAGTAC
    ATGTCGGATC TAGAAACCTG CATCGAAGAC TTCAAAAACT TATCCTTCAT CGAAAAGGAC
    ATATTCACGT CCTGGATTAA ATCCTACAAG TACTACGGAT ATCACACGAA GCTCAATCTG
    AGCGCAGAAG ACGTCTTCAA AGAGAATTTG GGAACGTTTC TACGGTTTTA CTCTGATTTC
    AAACAGGACG TTAACTTCAC CAACAACTCC ATCCACGCGT CCCGGTTTTT CATACAGACC
    GTAAACATCT CCACTGCCCT GGATGAAATG AACATGTTAA ATAAACTACG AGACACTGCC
    CAAAAGTGCC CTGTTCCCTT ATTGGTGTAC CACCCTGCCT TCATTTACCA CGACCAGTAT
    GCGGTCATAG TCACCAACAC CATCCAAAAC ATTGCGGTTA CCACAGCCGT GATGCTTCTG
    ATCTCCCTTC TCCTCATCCC GAATCCTCTG TGCTCCCTTT GGGTCACTTT CTCCATTGCA
    TCCGTCATCG TCGGAGTCAC TGGGTTCATG GCGTTATGGG ATGTCAACTT GGACACCATT
    TCCATGATCA TTCTTGTCGT CTGCATTGGG TTCTCCGTAG ACTTTTCTGC TCATATTTCA
    TATGCCTTTG TATCCAACAA GAAGCCCAGT GCGAACGAAA AAGCAGTGGA GGCTTTGTTT
    AATTTAGGAT ACCCCATTTT GCAGGGTGCT GTGTCCACTA TTCTTGGGGT GGTTGTCTTG
    TCTGCGTCCA AGAATTACAT TTTTAGGACT TTCTTTAAAA TTATGTTCTT GGTCATCTTC
    TTTGGGCTCT TCCATGGTTT GACTTTCATC CCTGTTTTCT TGACCTTCTT TGATATGTGT
    AACGGAACGC CGGCTGAAAA CAAATCAGGC CCGGAAGAGC AAGCCATGAA GAAGAGTTAC
    ACCAATAACA TTGCTAAGGA TGGTGACGTG AAAGAGCGGG AAATATATGA CAATCACACT
    TTCCAATCTG ACAACCATCA AACATGTCCG TCTCAGCCCT GCTCTGATAT ATGGATGATA
    GCCGGAAATA ATCATAGTGT GCAGGATAAT CACGTGTGCT TGGCGAGCAC TGTCCCAATG
    TTCAGAGTTG ACGGTCAGAC CTATGAGGTT CACATGTATG AACCGTCCGA TGATGTCATG
    TTAAAAGTCA ACTGTCAATC AAAACAGCGT GTCGAAGGAC AAAATTGTGT TTCGGGAAAC
    AGCAACCACA TTCAAGATTC AGGTCTTCCT ACTCCTAACT GTTCCGAGTT GACCATTTTG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003201375.2
    CDS264..3206
    Translation

    Target ORF information:

    RefSeq Version XM_003201327.4
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio patched domain containing 3a (ptchd3a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003201327.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAGCAAA ATGAGAAGAA ACCACAACGT AAACACTGCG TGGAGAAGCC GATCTCCATC 
    TGCTTCCAGA AGTTCGGCCG TTTTGTGGGC ACTTACCCCT GGTGGTTCTT CATCTCTCCC
    CTGCTCATCT CTGCTGTGTT AGGCACTGGC TTTTGCCTTC TAGAAGATCG TGAAGCCAAT
    GACATTGAGG ATCAGTTCAC ACCTGTAAAC GGTCCTGCCA AGCAGGAGAG GCGCTTTGTG
    CAGCAGAACT TTCCGCAGAA CGACTCGCTG TTCTCCAACC AAAGGCTCTA CACGGACGGG
    GTTTACGCGT CCTTCATAGC CGTGACTAAA TCCTCCAATA TCCTCACAGA CGCTGCTTTT
    CGGGAGATAG TGATTTTAGA CAGGAAAGTC AAGGAGCTGA ATGTGTCCAC GGGTCACGAG
    GTGCTTACTT TTGAGGGACT TTGCGCAAGA CGATACAAGA AGTGCATACC CAATAAGATA
    TTGGACATCC ATAAGTATTA CGGAGAGAAT CTGGAGGCCA CCGAGCTCTC CTTTCCGTTC
    TTCCGCTTCG GATTCTCCTA CATTTTCTTG GGTTATTCGG TCGGTGGAGT GAATTTGAAC
    TCTTCCATTA TTAAAAGCGC AAAGTCAGTG CGTCTGTTTT ATTTTCTAAA GGAGGATAAT
    CGTACTAGAA CTGATTTATG GCTGAATGAG TTCCTCAAGG TTTTCCCGTC TAATTTATCG
    CTAAACTCCA TAAAGGTGAC CCACTCCACA TCTTTGTCAA GGCAGGTGGA GTTTGAGGCA
    AACACAAAAG ATGTCATTCC TCTCTTCTCC ATCACTTACG TCATCGCTAT TGCTTTCTCC
    ATTCTTTCTT GCTTGAGGTT CGACTGTGTC AGAAACAAAG TGTGGGTTGC AACATTTGGG
    GTTTTCTCTG CTGGACTGGC TGTGTTGTCT TCTTTCGGCA TGATGTTACA CATTGGAGTC
    CCTTTTGTCA TGACAGTTGC TAATTCTCCA TTCCTAATAT TAGGGATTGG AGTAGACGAC
    ATGTTCATCC TCATCTCCTG TTGGCAGCAG ACGAATGTTC ACGATCGAGT GGAGACTCGC
    CTGTCTAATA CATACAAAGA GGCGGCCATT TCAATCACCA TCACCACTCT CACAGACGTA
    CTCGCTTTCT ACATCGGCCT CATGACGCCT TTCCGCTCCG TCCGCTCTTT TTGCCTGTAC
    ACCAGCACCT CCATCCTTTT CTGCTACATC TACAGCATCA CTTTTTTTGG AGCGTTCCTC
    GTCCTTAACG GACGGCGGGA GAACAGCAAC AAGCACTGGC TGACCTGCAA AGAAGTACCC
    GAAGAGTGTG CTGTAGGACA GTCAAAATGG TACGAATTAT GTTGCATTGG AGGAGCTTAC
    GACCGCCATA CTGGCTCAGA GGAGGTCCAA CCCATGAATC ACTTCTTCAA AAAATACTAC
    GGCCCATTTC TGACCAAATC CTGGACGAAG GTGTTTGTGA TCGTCTTTTA TTGCATCTAT
    TTAATCGTCA GCATTTACGG CTGCTTTCAG ATTCAGGAAG GAATCGATCT CCGCAATTTG
    GCGGCTGACG ATTCCTACGT GGTAAAATAC TACGATAACG AGAAGGCGTA TTTTTCCGAA
    TACGGCCCAA ACATCATGGT TGTCATCCGA CAGGAATTTC CATACTGGGA TGAAAAGTAC
    ATGTCGGATC TAGAAACCTG CATCGAAGAC TTCAAAAACT TATCCTTCAT CGAAAAGGAC
    ATATTCACGT CCTGGATTAA ATCCTACAAG TACTACGGAT ATCACACGAA GCTCAATCTG
    AGCGCAGAAG ACGTCTTCAA AGAGAATTTG GGAACGTTTC TACGGTTTTA CTCTGATTTC
    AAACAGGACG TTAACTTCAC CAACAACTCC ATCCACGCGT CCCGGTTTTT CATACAGACC
    GTAAACATCT CCACTGCCCT GGATGAAATG AACATGTTAA ATAAACTACG AGACACTGCC
    CAAAAGTGCC CTGTTCCCTT ATTGGTGTAC CACCCTGCCT TCATTTACCA CGACCAGTAT
    GCGGTCATAG TCACCAACAC CATCCAAAAC ATTGCGGTTA CCACAGCCGT GATGCTTCTG
    ATCTCCCTTC TCCTCATCCC GAATCCTCTG TGCTCCCTTT GGGTCACTTT CTCCATTGCA
    TCCGTCATCG TCGGAGTCAC TGGGTTCATG GCGTTATGGG ATGTCAACTT GGACACCATT
    TCCATGATCA TTCTTGTCGT CTGCATTGGG TTCTCCGTAG ACTTTTCTGC TCATATTTCA
    TATGCCTTTG TATCCAACAA GAAGCCCAGT GCGAACGAAA AAGCAGTGGA GGCTTTGTTT
    AATTTAGGAT ACCCCATTTT GCAGGGTGCT GTGTCCACTA TTCTTGGGGT GGTTGTCTTG
    TCTGCGTCCA AGAATTACAT TTTTAGGACT TTCTTTAAAA TTATGTTCTT GGTCATCTTC
    TTTGGGCTCT TCCATGGTTT GACTTTCATC CCTGTTTTCT TGACCTTCTT TGATATGTGT
    AACGGAACGC CGGCTGAAAA CAAATCAGGC CCGGAAGAGC AAGCCATGAA GAAGAGTTAC
    ACCAATAACA TTGCTAAGGA TGGTGACGTG AAAGAGCGGG AAATATATGA CAATCACACT
    TTCCAATCTG ACAACCATCA AACATGTCCG TCTCAGCCCT GCTCTGATAT ATGGATGATA
    GCCGGAAATA ATCATAGTGT GCAGGATAAT CACGTGTGCT TGGCGAGCAC TGTCCCAATG
    TTCAGAGTTG ACGGTCAGAC CTATGAGGTT CACATGTATG AACCGTCCGA TGATGTCATG
    TTAAAAGTCA ACTGTCAATC AAAACAGCGT GTCGAAGGAC AAAATTGTGT TTCGGGAAAC
    AGCAACCACA TTCAAGATTC AGGTCTTCCT ACTCCTAACT GTTCCGAGTT GACCATTTTG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa57750
    Clone ID Related Accession (Same CDS sequence) XM_017354003.1 , XM_017354003.2
    Accession Version XM_017354003.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2940bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product patched domain-containing protein 3-like isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGCCGGTGA ACATGGCTGG GTGCACCACA GACTGCGTGG AGAAGCCGAT CTCCATCTGC 
    TTCCAGAAGT TCGGCCGTTT TGTGGGCACT TACCCCTGGT GGTTCTTCAT CTCTCCCCTG
    CTCATCTCTG CTGTGTTAGG CACTGGCTTT TGCCTTCTAG AAGATCGTGA AGCCAATGAC
    ATTGAGGATC AGTTCACACC TGTAAACGGT CCTGCCAAGC AGGAGAGGCG CTTTGTGCAG
    CAGAACTTTC CGCAGAACGA CTCGCTGTTC TCCAACCAAA GGCTCTACAC GGACGGGGTT
    TACGCGTCCT TCATAGCCGT GACTAAATCC TCCAATATCC TCACAGACGC TGCTTTTCGG
    GAGATAGTGA TTTTAGACAG GAAAGTCAAG GAGCTGAATG TGTCCACGGG TCACGAGGTG
    CTTACTTTTG AGGGACTTTG CGCAAGACGA TACAAGAAGT GCATACCCAA TAAGATATTG
    GACATCCATA AGTATTACGG AGAGAATCTG GAGGCCACCG AGCTCTCCTT TCCGTTCTTC
    CGCTTCGGAT TCTCCTACAT TTTCTTGGGT TATTCGGTCG GTGGAGTGAA TTTGAACTCT
    TCCATTATTA AAAGCGCAAA GTCAGTGCGT CTGTTTTATT TTCTAAAGGA GGATAATCGT
    ACTAGAACTG ATTTATGGCT GAATGAGTTC CTCAAGGTTT TCCCGTCTAA TTTATCGCTA
    AACTCCATAA AGGTGACCCA CTCCACATCT TTGTCAAGGC AGGTGGAGTT TGAGGCAAAC
    ACAAAAGATG TCATTCCTCT CTTCTCCATC ACTTACGTCA TCGCTATTGC TTTCTCCATT
    CTTTCTTGCT TGAGGTTCGA CTGTGTCAGA AACAAAGTGT GGGTTGCAAC ATTTGGGGTT
    TTCTCTGCTG GACTGGCTGT GTTGTCTTCT TTCGGCATGA TGTTACACAT TGGAGTCCCT
    TTTGTCATGA CAGTTGCTAA TTCTCCATTC CTAATATTAG GGATTGGAGT AGACGACATG
    TTCATCCTCA TCTCCTGTTG GCAGCAGACG AATGTTCACG ATCGAGTGGA GACTCGCCTG
    TCTAATACAT ACAAAGAGGC GGCCATTTCA ATCACCATCA CCACTCTCAC AGACGTACTC
    GCTTTCTACA TCGGCCTCAT GACGCCTTTC CGCTCCGTCC GCTCTTTTTG CCTGTACACC
    AGCACCTCCA TCCTTTTCTG CTACATCTAC AGCATCACTT TTTTTGGAGC GTTCCTCGTC
    CTTAACGGAC GGCGGGAGAA CAGCAACAAG CACTGGCTGA CCTGCAAAGA AGTACCCGAA
    GAGTGTGCTG TAGGACAGTC AAAATGGTAC GAATTATGTT GCATTGGAGG AGCTTACGAC
    CGCCATACTG GCTCAGAGGA GGTCCAACCC ATGAATCACT TCTTCAAAAA ATACTACGGC
    CCATTTCTGA CCAAATCCTG GACGAAGGTG TTTGTGATCG TCTTTTATTG CATCTATTTA
    ATCGTCAGCA TTTACGGCTG CTTTCAGATT CAGGAAGGAA TCGATCTCCG CAATTTGGCG
    GCTGACGATT CCTACGTGGT AAAATACTAC GATAACGAGA AGGCGTATTT TTCCGAATAC
    GGCCCAAACA TCATGGTTGT CATCCGACAG GAATTTCCAT ACTGGGATGA AAAGTACATG
    TCGGATCTAG AAACCTGCAT CGAAGACTTC AAAAACTTAT CCTTCATCGA AAAGGACATA
    TTCACGTCCT GGATTAAATC CTACAAGTAC TACGGATATC ACACGAAGCT CAATCTGAGC
    GCAGAAGACG TCTTCAAAGA GAATTTGGGA ACGTTTCTAC GGTTTTACTC TGATTTCAAA
    CAGGACGTTA ACTTCACCAA CAACTCCATC CACGCGTCCC GGTTTTTCAT ACAGACCGTA
    AACATCTCCA CTGCCCTGGA TGAAATGAAC ATGTTAAATA AACTACGAGA CACTGCCCAA
    AAGTGCCCTG TTCCCTTATT GGTGTACCAC CCTGCCTTCA TTTACCACGA CCAGTATGCG
    GTCATAGTCA CCAACACCAT CCAAAACATT GCGGTTACCA CAGCCGTGAT GCTTCTGATC
    TCCCTTCTCC TCATCCCGAA TCCTCTGTGC TCCCTTTGGG TCACTTTCTC CATTGCATCC
    GTCATCGTCG GAGTCACTGG GTTCATGGCG TTATGGGATG TCAACTTGGA CACCATTTCC
    ATGATCATTC TTGTCGTCTG CATTGGGTTC TCCGTAGACT TTTCTGCTCA TATTTCATAT
    GCCTTTGTAT CCAACAAGAA GCCCAGTGCG AACGAAAAAG CAGTGGAGGC TTTGTTTAAT
    TTAGGATACC CCATTTTGCA GGGTGCTGTG TCCACTATTC TTGGGGTGGT TGTCTTGTCT
    GCGTCCAAGA ATTACATTTT TAGGACTTTC TTTAAAATTA TGTTCTTGGT CATCTTCTTT
    GGGCTCTTCC ATGGTTTGAC TTTCATCCCT GTTTTCTTGA CCTTCTTTGA TATGTGTAAC
    GGAACGCCGG CTGAAAACAA ATCAGGCCCG GAAGAGCAAG CCATGAAGAA GAGTTACACC
    AATAACATTG CTAAGGATGG TGACGTGAAA GAGCGGGAAA TATATGACAA TCACACTTTC
    CAATCTGACA ACCATCAAAC ATGTCCGTCT CAGCCCTGCT CTGATATATG GATGATAGCC
    GGAAATAATC ATAGTGTGCA GGATAATCAC GTGTGCTTGG CGAGCACTGT CCCAATGTTC
    AGAGTTGACG GTCAGACCTA TGAGGTTCAC ATGTATGAAC CGTCCGATGA TGTCATGTTA
    AAAGTCAACT GTCAATCAAA ACAGCGTGTC GAAGGACAAA ATTGTGTTTC GGGAAACAGC
    AACCACATTC AAGATTCAGG TCTTCCTACT CCTAACTGTT CCGAGTTGAC CATTTTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017209492.1
    CDS155..3094
    Translation

    Target ORF information:

    RefSeq Version XM_017354003.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio patched domain containing 3a (ptchd3a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017354003.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGCCGGTGA ACATGGCTGG GTGCACCACA GACTGCGTGG AGAAGCCGAT CTCCATCTGC 
    TTCCAGAAGT TCGGCCGTTT TGTGGGCACT TACCCCTGGT GGTTCTTCAT CTCTCCCCTG
    CTCATCTCTG CTGTGTTAGG CACTGGCTTT TGCCTTCTAG AAGATCGTGA AGCCAATGAC
    ATTGAGGATC AGTTCACACC TGTAAACGGT CCTGCCAAGC AGGAGAGGCG CTTTGTGCAG
    CAGAACTTTC CGCAGAACGA CTCGCTGTTC TCCAACCAAA GGCTCTACAC GGACGGGGTT
    TACGCGTCCT TCATAGCCGT GACTAAATCC TCCAATATCC TCACAGACGC TGCTTTTCGG
    GAGATAGTGA TTTTAGACAG GAAAGTCAAG GAGCTGAATG TGTCCACGGG TCACGAGGTG
    CTTACTTTTG AGGGACTTTG CGCAAGACGA TACAAGAAGT GCATACCCAA TAAGATATTG
    GACATCCATA AGTATTACGG AGAGAATCTG GAGGCCACCG AGCTCTCCTT TCCGTTCTTC
    CGCTTCGGAT TCTCCTACAT TTTCTTGGGT TATTCGGTCG GTGGAGTGAA TTTGAACTCT
    TCCATTATTA AAAGCGCAAA GTCAGTGCGT CTGTTTTATT TTCTAAAGGA GGATAATCGT
    ACTAGAACTG ATTTATGGCT GAATGAGTTC CTCAAGGTTT TCCCGTCTAA TTTATCGCTA
    AACTCCATAA AGGTGACCCA CTCCACATCT TTGTCAAGGC AGGTGGAGTT TGAGGCAAAC
    ACAAAAGATG TCATTCCTCT CTTCTCCATC ACTTACGTCA TCGCTATTGC TTTCTCCATT
    CTTTCTTGCT TGAGGTTCGA CTGTGTCAGA AACAAAGTGT GGGTTGCAAC ATTTGGGGTT
    TTCTCTGCTG GACTGGCTGT GTTGTCTTCT TTCGGCATGA TGTTACACAT TGGAGTCCCT
    TTTGTCATGA CAGTTGCTAA TTCTCCATTC CTAATATTAG GGATTGGAGT AGACGACATG
    TTCATCCTCA TCTCCTGTTG GCAGCAGACG AATGTTCACG ATCGAGTGGA GACTCGCCTG
    TCTAATACAT ACAAAGAGGC GGCCATTTCA ATCACCATCA CCACTCTCAC AGACGTACTC
    GCTTTCTACA TCGGCCTCAT GACGCCTTTC CGCTCCGTCC GCTCTTTTTG CCTGTACACC
    AGCACCTCCA TCCTTTTCTG CTACATCTAC AGCATCACTT TTTTTGGAGC GTTCCTCGTC
    CTTAACGGAC GGCGGGAGAA CAGCAACAAG CACTGGCTGA CCTGCAAAGA AGTACCCGAA
    GAGTGTGCTG TAGGACAGTC AAAATGGTAC GAATTATGTT GCATTGGAGG AGCTTACGAC
    CGCCATACTG GCTCAGAGGA GGTCCAACCC ATGAATCACT TCTTCAAAAA ATACTACGGC
    CCATTTCTGA CCAAATCCTG GACGAAGGTG TTTGTGATCG TCTTTTATTG CATCTATTTA
    ATCGTCAGCA TTTACGGCTG CTTTCAGATT CAGGAAGGAA TCGATCTCCG CAATTTGGCG
    GCTGACGATT CCTACGTGGT AAAATACTAC GATAACGAGA AGGCGTATTT TTCCGAATAC
    GGCCCAAACA TCATGGTTGT CATCCGACAG GAATTTCCAT ACTGGGATGA AAAGTACATG
    TCGGATCTAG AAACCTGCAT CGAAGACTTC AAAAACTTAT CCTTCATCGA AAAGGACATA
    TTCACGTCCT GGATTAAATC CTACAAGTAC TACGGATATC ACACGAAGCT CAATCTGAGC
    GCAGAAGACG TCTTCAAAGA GAATTTGGGA ACGTTTCTAC GGTTTTACTC TGATTTCAAA
    CAGGACGTTA ACTTCACCAA CAACTCCATC CACGCGTCCC GGTTTTTCAT ACAGACCGTA
    AACATCTCCA CTGCCCTGGA TGAAATGAAC ATGTTAAATA AACTACGAGA CACTGCCCAA
    AAGTGCCCTG TTCCCTTATT GGTGTACCAC CCTGCCTTCA TTTACCACGA CCAGTATGCG
    GTCATAGTCA CCAACACCAT CCAAAACATT GCGGTTACCA CAGCCGTGAT GCTTCTGATC
    TCCCTTCTCC TCATCCCGAA TCCTCTGTGC TCCCTTTGGG TCACTTTCTC CATTGCATCC
    GTCATCGTCG GAGTCACTGG GTTCATGGCG TTATGGGATG TCAACTTGGA CACCATTTCC
    ATGATCATTC TTGTCGTCTG CATTGGGTTC TCCGTAGACT TTTCTGCTCA TATTTCATAT
    GCCTTTGTAT CCAACAAGAA GCCCAGTGCG AACGAAAAAG CAGTGGAGGC TTTGTTTAAT
    TTAGGATACC CCATTTTGCA GGGTGCTGTG TCCACTATTC TTGGGGTGGT TGTCTTGTCT
    GCGTCCAAGA ATTACATTTT TAGGACTTTC TTTAAAATTA TGTTCTTGGT CATCTTCTTT
    GGGCTCTTCC ATGGTTTGAC TTTCATCCCT GTTTTCTTGA CCTTCTTTGA TATGTGTAAC
    GGAACGCCGG CTGAAAACAA ATCAGGCCCG GAAGAGCAAG CCATGAAGAA GAGTTACACC
    AATAACATTG CTAAGGATGG TGACGTGAAA GAGCGGGAAA TATATGACAA TCACACTTTC
    CAATCTGACA ACCATCAAAC ATGTCCGTCT CAGCCCTGCT CTGATATATG GATGATAGCC
    GGAAATAATC ATAGTGTGCA GGATAATCAC GTGTGCTTGG CGAGCACTGT CCCAATGTTC
    AGAGTTGACG GTCAGACCTA TGAGGTTCAC ATGTATGAAC CGTCCGATGA TGTCATGTTA
    AAAGTCAACT GTCAATCAAA ACAGCGTGTC GAAGGACAAA ATTGTGTTTC GGGAAACAGC
    AACCACATTC AAGATTCAGG TCTTCCTACT CCTAACTGTT CCGAGTTGAC CATTTTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa57750
    Clone ID Related Accession (Same CDS sequence) XM_017354003.1 , XM_017354003.2
    Accession Version XM_017354003.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2940bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product patched domain-containing protein 3-like isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017354003.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGCCGGTGA ACATGGCTGG GTGCACCACA GACTGCGTGG AGAAGCCGAT CTCCATCTGC 
    TTCCAGAAGT TCGGCCGTTT TGTGGGCACT TACCCCTGGT GGTTCTTCAT CTCTCCCCTG
    CTCATCTCTG CTGTGTTAGG CACTGGCTTT TGCCTTCTAG AAGATCGTGA AGCCAATGAC
    ATTGAGGATC AGTTCACACC TGTAAACGGT CCTGCCAAGC AGGAGAGGCG CTTTGTGCAG
    CAGAACTTTC CGCAGAACGA CTCGCTGTTC TCCAACCAAA GGCTCTACAC GGACGGGGTT
    TACGCGTCCT TCATAGCCGT GACTAAATCC TCCAATATCC TCACAGACGC TGCTTTTCGG
    GAGATAGTGA TTTTAGACAG GAAAGTCAAG GAGCTGAATG TGTCCACGGG TCACGAGGTG
    CTTACTTTTG AGGGACTTTG CGCAAGACGA TACAAGAAGT GCATACCCAA TAAGATATTG
    GACATCCATA AGTATTACGG AGAGAATCTG GAGGCCACCG AGCTCTCCTT TCCGTTCTTC
    CGCTTCGGAT TCTCCTACAT TTTCTTGGGT TATTCGGTCG GTGGAGTGAA TTTGAACTCT
    TCCATTATTA AAAGCGCAAA GTCAGTGCGT CTGTTTTATT TTCTAAAGGA GGATAATCGT
    ACTAGAACTG ATTTATGGCT GAATGAGTTC CTCAAGGTTT TCCCGTCTAA TTTATCGCTA
    AACTCCATAA AGGTGACCCA CTCCACATCT TTGTCAAGGC AGGTGGAGTT TGAGGCAAAC
    ACAAAAGATG TCATTCCTCT CTTCTCCATC ACTTACGTCA TCGCTATTGC TTTCTCCATT
    CTTTCTTGCT TGAGGTTCGA CTGTGTCAGA AACAAAGTGT GGGTTGCAAC ATTTGGGGTT
    TTCTCTGCTG GACTGGCTGT GTTGTCTTCT TTCGGCATGA TGTTACACAT TGGAGTCCCT
    TTTGTCATGA CAGTTGCTAA TTCTCCATTC CTAATATTAG GGATTGGAGT AGACGACATG
    TTCATCCTCA TCTCCTGTTG GCAGCAGACG AATGTTCACG ATCGAGTGGA GACTCGCCTG
    TCTAATACAT ACAAAGAGGC GGCCATTTCA ATCACCATCA CCACTCTCAC AGACGTACTC
    GCTTTCTACA TCGGCCTCAT GACGCCTTTC CGCTCCGTCC GCTCTTTTTG CCTGTACACC
    AGCACCTCCA TCCTTTTCTG CTACATCTAC AGCATCACTT TTTTTGGAGC GTTCCTCGTC
    CTTAACGGAC GGCGGGAGAA CAGCAACAAG CACTGGCTGA CCTGCAAAGA AGTACCCGAA
    GAGTGTGCTG TAGGACAGTC AAAATGGTAC GAATTATGTT GCATTGGAGG AGCTTACGAC
    CGCCATACTG GCTCAGAGGA GGTCCAACCC ATGAATCACT TCTTCAAAAA ATACTACGGC
    CCATTTCTGA CCAAATCCTG GACGAAGGTG TTTGTGATCG TCTTTTATTG CATCTATTTA
    ATCGTCAGCA TTTACGGCTG CTTTCAGATT CAGGAAGGAA TCGATCTCCG CAATTTGGCG
    GCTGACGATT CCTACGTGGT AAAATACTAC GATAACGAGA AGGCGTATTT TTCCGAATAC
    GGCCCAAACA TCATGGTTGT CATCCGACAG GAATTTCCAT ACTGGGATGA AAAGTACATG
    TCGGATCTAG AAACCTGCAT CGAAGACTTC AAAAACTTAT CCTTCATCGA AAAGGACATA
    TTCACGTCCT GGATTAAATC CTACAAGTAC TACGGATATC ACACGAAGCT CAATCTGAGC
    GCAGAAGACG TCTTCAAAGA GAATTTGGGA ACGTTTCTAC GGTTTTACTC TGATTTCAAA
    CAGGACGTTA ACTTCACCAA CAACTCCATC CACGCGTCCC GGTTTTTCAT ACAGACCGTA
    AACATCTCCA CTGCCCTGGA TGAAATGAAC ATGTTAAATA AACTACGAGA CACTGCCCAA
    AAGTGCCCTG TTCCCTTATT GGTGTACCAC CCTGCCTTCA TTTACCACGA CCAGTATGCG
    GTCATAGTCA CCAACACCAT CCAAAACATT GCGGTTACCA CAGCCGTGAT GCTTCTGATC
    TCCCTTCTCC TCATCCCGAA TCCTCTGTGC TCCCTTTGGG TCACTTTCTC CATTGCATCC
    GTCATCGTCG GAGTCACTGG GTTCATGGCG TTATGGGATG TCAACTTGGA CACCATTTCC
    ATGATCATTC TTGTCGTCTG CATTGGGTTC TCCGTAGACT TTTCTGCTCA TATTTCATAT
    GCCTTTGTAT CCAACAAGAA GCCCAGTGCG AACGAAAAAG CAGTGGAGGC TTTGTTTAAT
    TTAGGATACC CCATTTTGCA GGGTGCTGTG TCCACTATTC TTGGGGTGGT TGTCTTGTCT
    GCGTCCAAGA ATTACATTTT TAGGACTTTC TTTAAAATTA TGTTCTTGGT CATCTTCTTT
    GGGCTCTTCC ATGGTTTGAC TTTCATCCCT GTTTTCTTGA CCTTCTTTGA TATGTGTAAC
    GGAACGCCGG CTGAAAACAA ATCAGGCCCG GAAGAGCAAG CCATGAAGAA GAGTTACACC
    AATAACATTG CTAAGGATGG TGACGTGAAA GAGCGGGAAA TATATGACAA TCACACTTTC
    CAATCTGACA ACCATCAAAC ATGTCCGTCT CAGCCCTGCT CTGATATATG GATGATAGCC
    GGAAATAATC ATAGTGTGCA GGATAATCAC GTGTGCTTGG CGAGCACTGT CCCAATGTTC
    AGAGTTGACG GTCAGACCTA TGAGGTTCAC ATGTATGAAC CGTCCGATGA TGTCATGTTA
    AAAGTCAACT GTCAATCAAA ACAGCGTGTC GAAGGACAAA ATTGTGTTTC GGGAAACAGC
    AACCACATTC AAGATTCAGG TCTTCCTACT CCTAACTGTT CCGAGTTGAC CATTTTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017209492.1
    CDS242..3181
    Translation

    Target ORF information:

    RefSeq Version XM_017354003.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio patched domain containing 3a (ptchd3a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017354003.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGCCGGTGA ACATGGCTGG GTGCACCACA GACTGCGTGG AGAAGCCGAT CTCCATCTGC 
    TTCCAGAAGT TCGGCCGTTT TGTGGGCACT TACCCCTGGT GGTTCTTCAT CTCTCCCCTG
    CTCATCTCTG CTGTGTTAGG CACTGGCTTT TGCCTTCTAG AAGATCGTGA AGCCAATGAC
    ATTGAGGATC AGTTCACACC TGTAAACGGT CCTGCCAAGC AGGAGAGGCG CTTTGTGCAG
    CAGAACTTTC CGCAGAACGA CTCGCTGTTC TCCAACCAAA GGCTCTACAC GGACGGGGTT
    TACGCGTCCT TCATAGCCGT GACTAAATCC TCCAATATCC TCACAGACGC TGCTTTTCGG
    GAGATAGTGA TTTTAGACAG GAAAGTCAAG GAGCTGAATG TGTCCACGGG TCACGAGGTG
    CTTACTTTTG AGGGACTTTG CGCAAGACGA TACAAGAAGT GCATACCCAA TAAGATATTG
    GACATCCATA AGTATTACGG AGAGAATCTG GAGGCCACCG AGCTCTCCTT TCCGTTCTTC
    CGCTTCGGAT TCTCCTACAT TTTCTTGGGT TATTCGGTCG GTGGAGTGAA TTTGAACTCT
    TCCATTATTA AAAGCGCAAA GTCAGTGCGT CTGTTTTATT TTCTAAAGGA GGATAATCGT
    ACTAGAACTG ATTTATGGCT GAATGAGTTC CTCAAGGTTT TCCCGTCTAA TTTATCGCTA
    AACTCCATAA AGGTGACCCA CTCCACATCT TTGTCAAGGC AGGTGGAGTT TGAGGCAAAC
    ACAAAAGATG TCATTCCTCT CTTCTCCATC ACTTACGTCA TCGCTATTGC TTTCTCCATT
    CTTTCTTGCT TGAGGTTCGA CTGTGTCAGA AACAAAGTGT GGGTTGCAAC ATTTGGGGTT
    TTCTCTGCTG GACTGGCTGT GTTGTCTTCT TTCGGCATGA TGTTACACAT TGGAGTCCCT
    TTTGTCATGA CAGTTGCTAA TTCTCCATTC CTAATATTAG GGATTGGAGT AGACGACATG
    TTCATCCTCA TCTCCTGTTG GCAGCAGACG AATGTTCACG ATCGAGTGGA GACTCGCCTG
    TCTAATACAT ACAAAGAGGC GGCCATTTCA ATCACCATCA CCACTCTCAC AGACGTACTC
    GCTTTCTACA TCGGCCTCAT GACGCCTTTC CGCTCCGTCC GCTCTTTTTG CCTGTACACC
    AGCACCTCCA TCCTTTTCTG CTACATCTAC AGCATCACTT TTTTTGGAGC GTTCCTCGTC
    CTTAACGGAC GGCGGGAGAA CAGCAACAAG CACTGGCTGA CCTGCAAAGA AGTACCCGAA
    GAGTGTGCTG TAGGACAGTC AAAATGGTAC GAATTATGTT GCATTGGAGG AGCTTACGAC
    CGCCATACTG GCTCAGAGGA GGTCCAACCC ATGAATCACT TCTTCAAAAA ATACTACGGC
    CCATTTCTGA CCAAATCCTG GACGAAGGTG TTTGTGATCG TCTTTTATTG CATCTATTTA
    ATCGTCAGCA TTTACGGCTG CTTTCAGATT CAGGAAGGAA TCGATCTCCG CAATTTGGCG
    GCTGACGATT CCTACGTGGT AAAATACTAC GATAACGAGA AGGCGTATTT TTCCGAATAC
    GGCCCAAACA TCATGGTTGT CATCCGACAG GAATTTCCAT ACTGGGATGA AAAGTACATG
    TCGGATCTAG AAACCTGCAT CGAAGACTTC AAAAACTTAT CCTTCATCGA AAAGGACATA
    TTCACGTCCT GGATTAAATC CTACAAGTAC TACGGATATC ACACGAAGCT CAATCTGAGC
    GCAGAAGACG TCTTCAAAGA GAATTTGGGA ACGTTTCTAC GGTTTTACTC TGATTTCAAA
    CAGGACGTTA ACTTCACCAA CAACTCCATC CACGCGTCCC GGTTTTTCAT ACAGACCGTA
    AACATCTCCA CTGCCCTGGA TGAAATGAAC ATGTTAAATA AACTACGAGA CACTGCCCAA
    AAGTGCCCTG TTCCCTTATT GGTGTACCAC CCTGCCTTCA TTTACCACGA CCAGTATGCG
    GTCATAGTCA CCAACACCAT CCAAAACATT GCGGTTACCA CAGCCGTGAT GCTTCTGATC
    TCCCTTCTCC TCATCCCGAA TCCTCTGTGC TCCCTTTGGG TCACTTTCTC CATTGCATCC
    GTCATCGTCG GAGTCACTGG GTTCATGGCG TTATGGGATG TCAACTTGGA CACCATTTCC
    ATGATCATTC TTGTCGTCTG CATTGGGTTC TCCGTAGACT TTTCTGCTCA TATTTCATAT
    GCCTTTGTAT CCAACAAGAA GCCCAGTGCG AACGAAAAAG CAGTGGAGGC TTTGTTTAAT
    TTAGGATACC CCATTTTGCA GGGTGCTGTG TCCACTATTC TTGGGGTGGT TGTCTTGTCT
    GCGTCCAAGA ATTACATTTT TAGGACTTTC TTTAAAATTA TGTTCTTGGT CATCTTCTTT
    GGGCTCTTCC ATGGTTTGAC TTTCATCCCT GTTTTCTTGA CCTTCTTTGA TATGTGTAAC
    GGAACGCCGG CTGAAAACAA ATCAGGCCCG GAAGAGCAAG CCATGAAGAA GAGTTACACC
    AATAACATTG CTAAGGATGG TGACGTGAAA GAGCGGGAAA TATATGACAA TCACACTTTC
    CAATCTGACA ACCATCAAAC ATGTCCGTCT CAGCCCTGCT CTGATATATG GATGATAGCC
    GGAAATAATC ATAGTGTGCA GGATAATCAC GTGTGCTTGG CGAGCACTGT CCCAATGTTC
    AGAGTTGACG GTCAGACCTA TGAGGTTCAC ATGTATGAAC CGTCCGATGA TGTCATGTTA
    AAAGTCAACT GTCAATCAAA ACAGCGTGTC GAAGGACAAA ATTGTGTTTC GGGAAACAGC
    AACCACATTC AAGATTCAGG TCTTCCTACT CCTAACTGTT CCGAGTTGAC CATTTTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.