MAP3K1 cDNA ORF clone, Danio rerio(Zebrafish)

The following MAP3K1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAP3K1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa15816 XM_005155507.4
Latest version!
Danio rerio mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (map3k1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa49967 XM_009305108.2
Latest version!
Danio rerio mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (map3k1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
ODa15816 XM_005155507.3 Danio rerio mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (map3k1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa15816
    Clone ID Related Accession (Same CDS sequence) XM_005155507.4 , XM_005155507.3
    Accession Version XM_005155507.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4440bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product mitogen-activated protein kinase kinase kinase 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005155507.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCGACGG CGGGGAGCCG GACGTCGTCG TTAGGCGGAT TATTAGACTC CAGTAGCGGC 
    ACAAACGTCG GGAACGGACT TCAAGGCACT TCCAACGGCT CCACGTCTCA GAACAATAGC
    GGGAAAGTGA AGAGAAATGC GGGTGAGAGC TCTCAGTGGA GACGAAAGGT GAGGAGTGTG
    GATCTGGATA AATGCGAGAG CGGGACCGCA GCGCTGCACT TCCTCACGGT GTCCGGTCCC
    GGCTCGATCC AGACCGCTTT ACTGCAGCAG AGCCTCGCCG ACCCGCCGCT CGGTGACAAA
    ATAAGCAATA ATTTGTGTAC AAGTGCTCAG GAATGGATGA AAAGCTCTTT AAAAAGCGGC
    AGTGAAAGTC CGAGCGCTGA ACATGGAGGG GCTGCTGCTG CTGTGGACGT GAACGCTTCG
    CAAAACAGCC GGGACATGGA GAACAAGGAG ACTCTGCGCG GGCTGCAGAA GATGGAGGAC
    CGGCCTGAGG AGCGACTGAT CCGAGAGAAG CTCAAGGCCA CCTGTATGCC CACCTGGAAG
    AGCGAGTGGC TGGAGAGGCG GAGCAGGAGA GGACCAATGG TTGTGAAGCC CATTCCTCTG
    AGAGCCGACG GCACTGAAGC CGGGAGTGAC AGTCACAACT CTTCTTCATC CCAGAGCCGA
    GGTCAACGCA GCCCGTCTCC CGGACCTTCC TCTTTCTCAG CCGCTTCCTC CTCCTCATCA
    TCGTCCTCCA CAAAGACTGC TGGGAAGCCA GATTCTCCTG GCCTACGCAG ACGGAGAGCT
    TCACCAGTGC CCAGTGGACG TGTGACCCCT CCTCGAAGAG CTCCCTCCCC TGATGGCTTT
    TCCCCATACA GTCCAGAGGA GACCAGCCGC AGGGTCAACA AGGTCATGAG GGCGCGCCTG
    TACCTGCTCC AGCAAATAGG ACCCAACTCA TTCTTGATTG GCGGAGACAG TCCTGACAAC
    AAGTTCAGGG TGTTCATTGG TCCACAGACC TGCAGCTGCG GCAGAGGAAC TTTCTGCATC
    CATGTGCTCT TCGTCATGCT CCGAGTTTTC CAGCTGGAGC CTTCAGATCC TCTTCTGTGG
    CGGAAAACCC TCAAGAATTT TGAGGTGGAG AGCTTATTTC AGAAGTACCA CAATCGCCGC
    AGCTCCCGCA TCAAAGCTCC CTCACGCAGC ACCATCCAGA AGTTTGTGTC CCGCATGTCC
    AACTCTCACA CCTCGTGTAC CTCCAGTGCC TCACCATCCA GTAATGAAAG CAGTATGAAG
    GACGAGGAGG AGCAGATGTG TCCCATCTGT CTGCTGGACA TGCTGGATGA AGAAAGTCTG
    ACAGTGTGTG AAGAGGGCTG CCGAAACAAA CTCCACCATC ACTGCATGTC TATATGGGCC
    GAGGAGTGCA GAAGAAATCA CGATCCTTTA ATCTGCCCAC TGTGTAGAGC CAAATGGAAG
    CCTCATGATT TTTACAGTTA TGAAGCGCCA TCTACTGTGG AAAGCACAAC CAGCCGCTCT
    CAGAGTCAAA CAGCTGCTTC ATCATCATCA TCATCTTCAT CTCTTCATGC TGAGAGCTCC
    AGGACGGGCC AGGATGGAGA CTTCTCTCTT CCTCAATATG GAGTTCAGCA CATCCCTCAG
    ACGTACACGG AGCTCGCCGA ACCCTGGATC AAGGTGTTTG GATTGGAGCT GGTCGGCTGC
    CTCTTCTCTC GTAACTGGAA CATCAGAGAA ATGGCTCTGC GGCGGCTGTC CCATGACGTG
    AGTGGAGCCC TGCTCTTGGC CAACGGCGAG CACTCATTGG CCGGAGCGGG TGCAGGGGCA
    GGGCTGGCCG AGGTATCTGG AGACGTGGTG GTGGAGTCCT GCTGTAGTGT GCTCTCCATG
    GTCTGTGCAG ACCCTGTCTA CAAAGTCTAC GTAGCAGCGC TGAAAACCCT GCGAGCGATG
    CTTGTCTACA CCCCATGCCA CTCTGCTTCA GAGAGGACTC GATTACAGCA GCTTCTGCGG
    CCCGTGGTGG AAACCATACT GGTCAAGTGT GCAGATGCTA ACAGTCGCAC CAGTCAGCTG
    TCCGTGTCCA CTCTGCTGGA GCTGTGCAAA GGTCAAATGG GAGAACTCGC TGTGGGCAGA
    GAAATTCTCA AAGCAGGCTC TATTGGTATT GGTGGAGTTG ACTATGTCCT GAACTGCATC
    TTGGGCTCTA AAACGGAGGC CAATAACTGG CAAGCACTGC TGGGTCGGCT GTGTCTGATT
    GACAGGCTCT TACTGGAGTT TCCTGCTGAA TTCTACCCTC ACATCGTGTC AGGTGGAGAC
    TGCAACCAGG CGCAAAACGT TGTGGAGAGG TACCAGAAGC TGCTGCCTCT TCTAAGTTTT
    GCTCTGAAGT CCATTGATAA CTCTCACTCC ATGGTGGGAA AGCTTTCTCG TCGTGTCTTC
    CTGAGCGCTG CCCGTATGGT AGCTCGTGTG CCTCATGTCT TCGTTAAGCT ACTGGACATG
    CTCAACGTCA CCAGCTCCAC CCACTATGCA CGCATGCGCC GGCGTCTTCT GGCCATTGCG
    GAGGAGATGG ACATCCTGGA TGCGATTCAT CTGGGACTAG ATGACCTACA GAAAGAGCAC
    ACCTCACCCT CTTGTGCTTT CCTTGAACCC TCAGCCCCTC ATAACTCCCC ATGTACGACT
    GAAAGAAGCT CCCCCACCCA TGGTCTACAG CAGCCCGCCG CTAAGGTGGC ACTTTCAGGT
    CAAGCCAGTT CTACTCCTGT AGAGGACATA ACCGAGAGAC TCTCAAACAT AGTCGCTAGC
    CCTGCTGAAT TTCCAAAGCC CCCCATTCAG GCTCGAAACA GACCCGTTAG TCAGTGTCTG
    AACTCCCCCT CATCTGGTCA ACCCCCAAGC CTTCCTTCTC TGTCCATGGC CAATGCCTCA
    GCCTTCCCTC CAGAAAACCC CAAAGCCCTG CCACAGAGTT TTATTCCCAG CAAGTTTGCC
    CAGTCCTCAC TGGGTTCCCA ACGCAAGTTG CCTCTTCAGA AAGGTGCCTG TAGTAGTAAA
    GAACCAGAGA AGCTTCCATC CCCTGTGTTT ACTCAAGCTC GGCCACTGCC CCAGAGTCAG
    ATCCACCGGC CCAGACCTTC TCGACCCACT CCTGCTGCCT CATCTGATGG AGCTAAAGCT
    TTAAGCCCTT TGACAAAGGG CAACATGAAA CTGGACCTTC AGGAGGCCCG GTCCAGAGAT
    GATGTGGCCT GCAGTGGGGG ATCTGCTCTC ATCCCCAGTG AGGACACTGC CTTCACGCCA
    GTGGAGGAGA AGTTCCACGG TCTGGATGCT TCAGCCGATC TCAGCTCCAG CATGGAGGAC
    CTGCTGGAAG CCTCTATCCC TGCGGCAGAC AGCACGGTCA CCTTCCAGTC TGAAGTGGCA
    GTGCTGTCCC CCGAGCAGGC AAATGCAAAC GATGACACGT ACAGTGAAGA CGTGACCCAG
    AACCAGAAGT GCAAGGAGAA GATGGAAGCT GAAGAAGAGG AGGCATTGGC TGTAGTCATG
    GCCATGTCTG AGTCACAAGA CGCCCTACCT GTAATCCCAC AGTTACAGGT GGACAAAGGG
    GAGGACGTGA TCATTATTCA GGTGGATACA CCAGAGACTC TGCCTGGTCA TACCAAAGCA
    AAGCAGCCAT ACAGGGAGGG AAACGAATGG CTAAAAGGTC AACAGATTGG CCTGGGTGCC
    TTTTCGTCAT GTTACCAGGC TCAGGATGTG GGCACTGGGA CTCTGATGGC TGTCAAACAG
    GTGACGTACG TGAGGAACAC TTCATCAGAG CAGGAGGAGG TGGTTGAGGC GCTACGAGAG
    GAGATTCGCA TGATGGGTCT ACTAAACCAC CCACACATCA TCCGCATGCT GGGGGCAACC
    TGTGAGAAGA ACAACTACAA CCTTTTCGTA GAGTGGATGG CAGGTGGCTC TGTATCTCAT
    CTCTTGAATA AGTATGGCGC ATTTAAAGAA GGTGTGATCA TCAACTACAC GGAGCAGCTT
    CTGCGGGGTC TGGCCTACCT TCATGAGAAC CAGATCATCC ACCGCGACAT CAAAGGTGCC
    AATCTACTGA TTGACAGCAC AGGCCAGAGG CTGAGGATAG CTGATTTTGG GGCTGCAGCA
    CGTTTGGCTT CGAAAGGCAC TGGAGCAGGG GAGTTCCAGG GTCAACTTCT GGGAACCATT
    GCCTTCATGG CTCCGGAGGT GCTGAGAGGA CAGCAGTACG GCCGGAGCTG TGATGTCTGG
    AGTGTTGGCT GTGCCATCAT TGAGATGTCC TGTGCTAAAC CTCCCTGGAA CGCTGAAAAA
    CACTCCAACC ACCTGGCGCT CATTTTCAAG ATCGCCAGCG CCACCACAGC GCCCTCCATC
    CCACCACACT TGTCTCCAGG CCTGCGGGAC GTGACCCTGC GCTGTCTGGA GCTCCAGCCC
    ACCGACCGGC CGCCCTCCAG AGAGCTCCTG AAACACCCCG TCTTCAGACA CAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005155564.1
    CDS36..4475
    Translation

    Target ORF information:

    RefSeq Version XM_005155507.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (map3k1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005155507.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCGACGG CGGGGAGCCG GACGTCGTCG TTAGGCGGAT TATTAGACTC CAGTAGCGGC 
    ACAAACGTCG GGAACGGACT TCAAGGCACT TCCAACGGCT CCACGTCTCA GAACAATAGC
    GGGAAAGTGA AGAGAAATGC GGGTGAGAGC TCTCAGTGGA GACGAAAGGT GAGGAGTGTG
    GATCTGGATA AATGCGAGAG CGGGACCGCA GCGCTGCACT TCCTCACGGT GTCCGGTCCC
    GGCTCGATCC AGACCGCTTT ACTGCAGCAG AGCCTCGCCG ACCCGCCGCT CGGTGACAAA
    ATAAGCAATA ATTTGTGTAC AAGTGCTCAG GAATGGATGA AAAGCTCTTT AAAAAGCGGC
    AGTGAAAGTC CGAGCGCTGA ACATGGAGGG GCTGCTGCTG CTGTGGACGT GAACGCTTCG
    CAAAACAGCC GGGACATGGA GAACAAGGAG ACTCTGCGCG GGCTGCAGAA GATGGAGGAC
    CGGCCTGAGG AGCGACTGAT CCGAGAGAAG CTCAAGGCCA CCTGTATGCC CACCTGGAAG
    AGCGAGTGGC TGGAGAGGCG GAGCAGGAGA GGACCAATGG TTGTGAAGCC CATTCCTCTG
    AGAGCCGACG GCACTGAAGC CGGGAGTGAC AGTCACAACT CTTCTTCATC CCAGAGCCGA
    GGTCAACGCA GCCCGTCTCC CGGACCTTCC TCTTTCTCAG CCGCTTCCTC CTCCTCATCA
    TCGTCCTCCA CAAAGACTGC TGGGAAGCCA GATTCTCCTG GCCTACGCAG ACGGAGAGCT
    TCACCAGTGC CCAGTGGACG TGTGACCCCT CCTCGAAGAG CTCCCTCCCC TGATGGCTTT
    TCCCCATACA GTCCAGAGGA GACCAGCCGC AGGGTCAACA AGGTCATGAG GGCGCGCCTG
    TACCTGCTCC AGCAAATAGG ACCCAACTCA TTCTTGATTG GCGGAGACAG TCCTGACAAC
    AAGTTCAGGG TGTTCATTGG TCCACAGACC TGCAGCTGCG GCAGAGGAAC TTTCTGCATC
    CATGTGCTCT TCGTCATGCT CCGAGTTTTC CAGCTGGAGC CTTCAGATCC TCTTCTGTGG
    CGGAAAACCC TCAAGAATTT TGAGGTGGAG AGCTTATTTC AGAAGTACCA CAATCGCCGC
    AGCTCCCGCA TCAAAGCTCC CTCACGCAGC ACCATCCAGA AGTTTGTGTC CCGCATGTCC
    AACTCTCACA CCTCGTGTAC CTCCAGTGCC TCACCATCCA GTAATGAAAG CAGTATGAAG
    GACGAGGAGG AGCAGATGTG TCCCATCTGT CTGCTGGACA TGCTGGATGA AGAAAGTCTG
    ACAGTGTGTG AAGAGGGCTG CCGAAACAAA CTCCACCATC ACTGCATGTC TATATGGGCC
    GAGGAGTGCA GAAGAAATCA CGATCCTTTA ATCTGCCCAC TGTGTAGAGC CAAATGGAAG
    CCTCATGATT TTTACAGTTA TGAAGCGCCA TCTACTGTGG AAAGCACAAC CAGCCGCTCT
    CAGAGTCAAA CAGCTGCTTC ATCATCATCA TCATCTTCAT CTCTTCATGC TGAGAGCTCC
    AGGACGGGCC AGGATGGAGA CTTCTCTCTT CCTCAATATG GAGTTCAGCA CATCCCTCAG
    ACGTACACGG AGCTCGCCGA ACCCTGGATC AAGGTGTTTG GATTGGAGCT GGTCGGCTGC
    CTCTTCTCTC GTAACTGGAA CATCAGAGAA ATGGCTCTGC GGCGGCTGTC CCATGACGTG
    AGTGGAGCCC TGCTCTTGGC CAACGGCGAG CACTCATTGG CCGGAGCGGG TGCAGGGGCA
    GGGCTGGCCG AGGTATCTGG AGACGTGGTG GTGGAGTCCT GCTGTAGTGT GCTCTCCATG
    GTCTGTGCAG ACCCTGTCTA CAAAGTCTAC GTAGCAGCGC TGAAAACCCT GCGAGCGATG
    CTTGTCTACA CCCCATGCCA CTCTGCTTCA GAGAGGACTC GATTACAGCA GCTTCTGCGG
    CCCGTGGTGG AAACCATACT GGTCAAGTGT GCAGATGCTA ACAGTCGCAC CAGTCAGCTG
    TCCGTGTCCA CTCTGCTGGA GCTGTGCAAA GGTCAAATGG GAGAACTCGC TGTGGGCAGA
    GAAATTCTCA AAGCAGGCTC TATTGGTATT GGTGGAGTTG ACTATGTCCT GAACTGCATC
    TTGGGCTCTA AAACGGAGGC CAATAACTGG CAAGCACTGC TGGGTCGGCT GTGTCTGATT
    GACAGGCTCT TACTGGAGTT TCCTGCTGAA TTCTACCCTC ACATCGTGTC AGGTGGAGAC
    TGCAACCAGG CGCAAAACGT TGTGGAGAGG TACCAGAAGC TGCTGCCTCT TCTAAGTTTT
    GCTCTGAAGT CCATTGATAA CTCTCACTCC ATGGTGGGAA AGCTTTCTCG TCGTGTCTTC
    CTGAGCGCTG CCCGTATGGT AGCTCGTGTG CCTCATGTCT TCGTTAAGCT ACTGGACATG
    CTCAACGTCA CCAGCTCCAC CCACTATGCA CGCATGCGCC GGCGTCTTCT GGCCATTGCG
    GAGGAGATGG ACATCCTGGA TGCGATTCAT CTGGGACTAG ATGACCTACA GAAAGAGCAC
    ACCTCACCCT CTTGTGCTTT CCTTGAACCC TCAGCCCCTC ATAACTCCCC ATGTACGACT
    GAAAGAAGCT CCCCCACCCA TGGTCTACAG CAGCCCGCCG CTAAGGTGGC ACTTTCAGGT
    CAAGCCAGTT CTACTCCTGT AGAGGACATA ACCGAGAGAC TCTCAAACAT AGTCGCTAGC
    CCTGCTGAAT TTCCAAAGCC CCCCATTCAG GCTCGAAACA GACCCGTTAG TCAGTGTCTG
    AACTCCCCCT CATCTGGTCA ACCCCCAAGC CTTCCTTCTC TGTCCATGGC CAATGCCTCA
    GCCTTCCCTC CAGAAAACCC CAAAGCCCTG CCACAGAGTT TTATTCCCAG CAAGTTTGCC
    CAGTCCTCAC TGGGTTCCCA ACGCAAGTTG CCTCTTCAGA AAGGTGCCTG TAGTAGTAAA
    GAACCAGAGA AGCTTCCATC CCCTGTGTTT ACTCAAGCTC GGCCACTGCC CCAGAGTCAG
    ATCCACCGGC CCAGACCTTC TCGACCCACT CCTGCTGCCT CATCTGATGG AGCTAAAGCT
    TTAAGCCCTT TGACAAAGGG CAACATGAAA CTGGACCTTC AGGAGGCCCG GTCCAGAGAT
    GATGTGGCCT GCAGTGGGGG ATCTGCTCTC ATCCCCAGTG AGGACACTGC CTTCACGCCA
    GTGGAGGAGA AGTTCCACGG TCTGGATGCT TCAGCCGATC TCAGCTCCAG CATGGAGGAC
    CTGCTGGAAG CCTCTATCCC TGCGGCAGAC AGCACGGTCA CCTTCCAGTC TGAAGTGGCA
    GTGCTGTCCC CCGAGCAGGC AAATGCAAAC GATGACACGT ACAGTGAAGA CGTGACCCAG
    AACCAGAAGT GCAAGGAGAA GATGGAAGCT GAAGAAGAGG AGGCATTGGC TGTAGTCATG
    GCCATGTCTG AGTCACAAGA CGCCCTACCT GTAATCCCAC AGTTACAGGT GGACAAAGGG
    GAGGACGTGA TCATTATTCA GGTGGATACA CCAGAGACTC TGCCTGGTCA TACCAAAGCA
    AAGCAGCCAT ACAGGGAGGG AAACGAATGG CTAAAAGGTC AACAGATTGG CCTGGGTGCC
    TTTTCGTCAT GTTACCAGGC TCAGGATGTG GGCACTGGGA CTCTGATGGC TGTCAAACAG
    GTGACGTACG TGAGGAACAC TTCATCAGAG CAGGAGGAGG TGGTTGAGGC GCTACGAGAG
    GAGATTCGCA TGATGGGTCT ACTAAACCAC CCACACATCA TCCGCATGCT GGGGGCAACC
    TGTGAGAAGA ACAACTACAA CCTTTTCGTA GAGTGGATGG CAGGTGGCTC TGTATCTCAT
    CTCTTGAATA AGTATGGCGC ATTTAAAGAA GGTGTGATCA TCAACTACAC GGAGCAGCTT
    CTGCGGGGTC TGGCCTACCT TCATGAGAAC CAGATCATCC ACCGCGACAT CAAAGGTGCC
    AATCTACTGA TTGACAGCAC AGGCCAGAGG CTGAGGATAG CTGATTTTGG GGCTGCAGCA
    CGTTTGGCTT CGAAAGGCAC TGGAGCAGGG GAGTTCCAGG GTCAACTTCT GGGAACCATT
    GCCTTCATGG CTCCGGAGGT GCTGAGAGGA CAGCAGTACG GCCGGAGCTG TGATGTCTGG
    AGTGTTGGCT GTGCCATCAT TGAGATGTCC TGTGCTAAAC CTCCCTGGAA CGCTGAAAAA
    CACTCCAACC ACCTGGCGCT CATTTTCAAG ATCGCCAGCG CCACCACAGC GCCCTCCATC
    CCACCACACT TGTCTCCAGG CCTGCGGGAC GTGACCCTGC GCTGTCTGGA GCTCCAGCCC
    ACCGACCGGC CGCCCTCCAG AGAGCTCCTG AAACACCCCG TCTTCAGACA CAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa15816
    Clone ID Related Accession (Same CDS sequence) XM_005155507.4 , XM_005155507.3
    Accession Version XM_005155507.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4440bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product mitogen-activated protein kinase kinase kinase 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005155507.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCGACGG CGGGGAGCCG GACGTCGTCG TTAGGCGGAT TATTAGACTC CAGTAGCGGC 
    ACAAACGTCG GGAACGGACT TCAAGGCACT TCCAACGGCT CCACGTCTCA GAACAATAGC
    GGGAAAGTGA AGAGAAATGC GGGTGAGAGC TCTCAGTGGA GACGAAAGGT GAGGAGTGTG
    GATCTGGATA AATGCGAGAG CGGGACCGCA GCGCTGCACT TCCTCACGGT GTCCGGTCCC
    GGCTCGATCC AGACCGCTTT ACTGCAGCAG AGCCTCGCCG ACCCGCCGCT CGGTGACAAA
    ATAAGCAATA ATTTGTGTAC AAGTGCTCAG GAATGGATGA AAAGCTCTTT AAAAAGCGGC
    AGTGAAAGTC CGAGCGCTGA ACATGGAGGG GCTGCTGCTG CTGTGGACGT GAACGCTTCG
    CAAAACAGCC GGGACATGGA GAACAAGGAG ACTCTGCGCG GGCTGCAGAA GATGGAGGAC
    CGGCCTGAGG AGCGACTGAT CCGAGAGAAG CTCAAGGCCA CCTGTATGCC CACCTGGAAG
    AGCGAGTGGC TGGAGAGGCG GAGCAGGAGA GGACCAATGG TTGTGAAGCC CATTCCTCTG
    AGAGCCGACG GCACTGAAGC CGGGAGTGAC AGTCACAACT CTTCTTCATC CCAGAGCCGA
    GGTCAACGCA GCCCGTCTCC CGGACCTTCC TCTTTCTCAG CCGCTTCCTC CTCCTCATCA
    TCGTCCTCCA CAAAGACTGC TGGGAAGCCA GATTCTCCTG GCCTACGCAG ACGGAGAGCT
    TCACCAGTGC CCAGTGGACG TGTGACCCCT CCTCGAAGAG CTCCCTCCCC TGATGGCTTT
    TCCCCATACA GTCCAGAGGA GACCAGCCGC AGGGTCAACA AGGTCATGAG GGCGCGCCTG
    TACCTGCTCC AGCAAATAGG ACCCAACTCA TTCTTGATTG GCGGAGACAG TCCTGACAAC
    AAGTTCAGGG TGTTCATTGG TCCACAGACC TGCAGCTGCG GCAGAGGAAC TTTCTGCATC
    CATGTGCTCT TCGTCATGCT CCGAGTTTTC CAGCTGGAGC CTTCAGATCC TCTTCTGTGG
    CGGAAAACCC TCAAGAATTT TGAGGTGGAG AGCTTATTTC AGAAGTACCA CAATCGCCGC
    AGCTCCCGCA TCAAAGCTCC CTCACGCAGC ACCATCCAGA AGTTTGTGTC CCGCATGTCC
    AACTCTCACA CCTCGTGTAC CTCCAGTGCC TCACCATCCA GTAATGAAAG CAGTATGAAG
    GACGAGGAGG AGCAGATGTG TCCCATCTGT CTGCTGGACA TGCTGGATGA AGAAAGTCTG
    ACAGTGTGTG AAGAGGGCTG CCGAAACAAA CTCCACCATC ACTGCATGTC TATATGGGCC
    GAGGAGTGCA GAAGAAATCA CGATCCTTTA ATCTGCCCAC TGTGTAGAGC CAAATGGAAG
    CCTCATGATT TTTACAGTTA TGAAGCGCCA TCTACTGTGG AAAGCACAAC CAGCCGCTCT
    CAGAGTCAAA CAGCTGCTTC ATCATCATCA TCATCTTCAT CTCTTCATGC TGAGAGCTCC
    AGGACGGGCC AGGATGGAGA CTTCTCTCTT CCTCAATATG GAGTTCAGCA CATCCCTCAG
    ACGTACACGG AGCTCGCCGA ACCCTGGATC AAGGTGTTTG GATTGGAGCT GGTCGGCTGC
    CTCTTCTCTC GTAACTGGAA CATCAGAGAA ATGGCTCTGC GGCGGCTGTC CCATGACGTG
    AGTGGAGCCC TGCTCTTGGC CAACGGCGAG CACTCATTGG CCGGAGCGGG TGCAGGGGCA
    GGGCTGGCCG AGGTATCTGG AGACGTGGTG GTGGAGTCCT GCTGTAGTGT GCTCTCCATG
    GTCTGTGCAG ACCCTGTCTA CAAAGTCTAC GTAGCAGCGC TGAAAACCCT GCGAGCGATG
    CTTGTCTACA CCCCATGCCA CTCTGCTTCA GAGAGGACTC GATTACAGCA GCTTCTGCGG
    CCCGTGGTGG AAACCATACT GGTCAAGTGT GCAGATGCTA ACAGTCGCAC CAGTCAGCTG
    TCCGTGTCCA CTCTGCTGGA GCTGTGCAAA GGTCAAATGG GAGAACTCGC TGTGGGCAGA
    GAAATTCTCA AAGCAGGCTC TATTGGTATT GGTGGAGTTG ACTATGTCCT GAACTGCATC
    TTGGGCTCTA AAACGGAGGC CAATAACTGG CAAGCACTGC TGGGTCGGCT GTGTCTGATT
    GACAGGCTCT TACTGGAGTT TCCTGCTGAA TTCTACCCTC ACATCGTGTC AGGTGGAGAC
    TGCAACCAGG CGCAAAACGT TGTGGAGAGG TACCAGAAGC TGCTGCCTCT TCTAAGTTTT
    GCTCTGAAGT CCATTGATAA CTCTCACTCC ATGGTGGGAA AGCTTTCTCG TCGTGTCTTC
    CTGAGCGCTG CCCGTATGGT AGCTCGTGTG CCTCATGTCT TCGTTAAGCT ACTGGACATG
    CTCAACGTCA CCAGCTCCAC CCACTATGCA CGCATGCGCC GGCGTCTTCT GGCCATTGCG
    GAGGAGATGG ACATCCTGGA TGCGATTCAT CTGGGACTAG ATGACCTACA GAAAGAGCAC
    ACCTCACCCT CTTGTGCTTT CCTTGAACCC TCAGCCCCTC ATAACTCCCC ATGTACGACT
    GAAAGAAGCT CCCCCACCCA TGGTCTACAG CAGCCCGCCG CTAAGGTGGC ACTTTCAGGT
    CAAGCCAGTT CTACTCCTGT AGAGGACATA ACCGAGAGAC TCTCAAACAT AGTCGCTAGC
    CCTGCTGAAT TTCCAAAGCC CCCCATTCAG GCTCGAAACA GACCCGTTAG TCAGTGTCTG
    AACTCCCCCT CATCTGGTCA ACCCCCAAGC CTTCCTTCTC TGTCCATGGC CAATGCCTCA
    GCCTTCCCTC CAGAAAACCC CAAAGCCCTG CCACAGAGTT TTATTCCCAG CAAGTTTGCC
    CAGTCCTCAC TGGGTTCCCA ACGCAAGTTG CCTCTTCAGA AAGGTGCCTG TAGTAGTAAA
    GAACCAGAGA AGCTTCCATC CCCTGTGTTT ACTCAAGCTC GGCCACTGCC CCAGAGTCAG
    ATCCACCGGC CCAGACCTTC TCGACCCACT CCTGCTGCCT CATCTGATGG AGCTAAAGCT
    TTAAGCCCTT TGACAAAGGG CAACATGAAA CTGGACCTTC AGGAGGCCCG GTCCAGAGAT
    GATGTGGCCT GCAGTGGGGG ATCTGCTCTC ATCCCCAGTG AGGACACTGC CTTCACGCCA
    GTGGAGGAGA AGTTCCACGG TCTGGATGCT TCAGCCGATC TCAGCTCCAG CATGGAGGAC
    CTGCTGGAAG CCTCTATCCC TGCGGCAGAC AGCACGGTCA CCTTCCAGTC TGAAGTGGCA
    GTGCTGTCCC CCGAGCAGGC AAATGCAAAC GATGACACGT ACAGTGAAGA CGTGACCCAG
    AACCAGAAGT GCAAGGAGAA GATGGAAGCT GAAGAAGAGG AGGCATTGGC TGTAGTCATG
    GCCATGTCTG AGTCACAAGA CGCCCTACCT GTAATCCCAC AGTTACAGGT GGACAAAGGG
    GAGGACGTGA TCATTATTCA GGTGGATACA CCAGAGACTC TGCCTGGTCA TACCAAAGCA
    AAGCAGCCAT ACAGGGAGGG AAACGAATGG CTAAAAGGTC AACAGATTGG CCTGGGTGCC
    TTTTCGTCAT GTTACCAGGC TCAGGATGTG GGCACTGGGA CTCTGATGGC TGTCAAACAG
    GTGACGTACG TGAGGAACAC TTCATCAGAG CAGGAGGAGG TGGTTGAGGC GCTACGAGAG
    GAGATTCGCA TGATGGGTCT ACTAAACCAC CCACACATCA TCCGCATGCT GGGGGCAACC
    TGTGAGAAGA ACAACTACAA CCTTTTCGTA GAGTGGATGG CAGGTGGCTC TGTATCTCAT
    CTCTTGAATA AGTATGGCGC ATTTAAAGAA GGTGTGATCA TCAACTACAC GGAGCAGCTT
    CTGCGGGGTC TGGCCTACCT TCATGAGAAC CAGATCATCC ACCGCGACAT CAAAGGTGCC
    AATCTACTGA TTGACAGCAC AGGCCAGAGG CTGAGGATAG CTGATTTTGG GGCTGCAGCA
    CGTTTGGCTT CGAAAGGCAC TGGAGCAGGG GAGTTCCAGG GTCAACTTCT GGGAACCATT
    GCCTTCATGG CTCCGGAGGT GCTGAGAGGA CAGCAGTACG GCCGGAGCTG TGATGTCTGG
    AGTGTTGGCT GTGCCATCAT TGAGATGTCC TGTGCTAAAC CTCCCTGGAA CGCTGAAAAA
    CACTCCAACC ACCTGGCGCT CATTTTCAAG ATCGCCAGCG CCACCACAGC GCCCTCCATC
    CCACCACACT TGTCTCCAGG CCTGCGGGAC GTGACCCTGC GCTGTCTGGA GCTCCAGCCC
    ACCGACCGGC CGCCCTCCAG AGAGCTCCTG AAACACCCCG TCTTCAGACA CAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005155564.1
    CDS39..4478
    Translation

    Target ORF information:

    RefSeq Version XM_005155507.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (map3k1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005155507.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCGACGG CGGGGAGCCG GACGTCGTCG TTAGGCGGAT TATTAGACTC CAGTAGCGGC 
    ACAAACGTCG GGAACGGACT TCAAGGCACT TCCAACGGCT CCACGTCTCA GAACAATAGC
    GGGAAAGTGA AGAGAAATGC GGGTGAGAGC TCTCAGTGGA GACGAAAGGT GAGGAGTGTG
    GATCTGGATA AATGCGAGAG CGGGACCGCA GCGCTGCACT TCCTCACGGT GTCCGGTCCC
    GGCTCGATCC AGACCGCTTT ACTGCAGCAG AGCCTCGCCG ACCCGCCGCT CGGTGACAAA
    ATAAGCAATA ATTTGTGTAC AAGTGCTCAG GAATGGATGA AAAGCTCTTT AAAAAGCGGC
    AGTGAAAGTC CGAGCGCTGA ACATGGAGGG GCTGCTGCTG CTGTGGACGT GAACGCTTCG
    CAAAACAGCC GGGACATGGA GAACAAGGAG ACTCTGCGCG GGCTGCAGAA GATGGAGGAC
    CGGCCTGAGG AGCGACTGAT CCGAGAGAAG CTCAAGGCCA CCTGTATGCC CACCTGGAAG
    AGCGAGTGGC TGGAGAGGCG GAGCAGGAGA GGACCAATGG TTGTGAAGCC CATTCCTCTG
    AGAGCCGACG GCACTGAAGC CGGGAGTGAC AGTCACAACT CTTCTTCATC CCAGAGCCGA
    GGTCAACGCA GCCCGTCTCC CGGACCTTCC TCTTTCTCAG CCGCTTCCTC CTCCTCATCA
    TCGTCCTCCA CAAAGACTGC TGGGAAGCCA GATTCTCCTG GCCTACGCAG ACGGAGAGCT
    TCACCAGTGC CCAGTGGACG TGTGACCCCT CCTCGAAGAG CTCCCTCCCC TGATGGCTTT
    TCCCCATACA GTCCAGAGGA GACCAGCCGC AGGGTCAACA AGGTCATGAG GGCGCGCCTG
    TACCTGCTCC AGCAAATAGG ACCCAACTCA TTCTTGATTG GCGGAGACAG TCCTGACAAC
    AAGTTCAGGG TGTTCATTGG TCCACAGACC TGCAGCTGCG GCAGAGGAAC TTTCTGCATC
    CATGTGCTCT TCGTCATGCT CCGAGTTTTC CAGCTGGAGC CTTCAGATCC TCTTCTGTGG
    CGGAAAACCC TCAAGAATTT TGAGGTGGAG AGCTTATTTC AGAAGTACCA CAATCGCCGC
    AGCTCCCGCA TCAAAGCTCC CTCACGCAGC ACCATCCAGA AGTTTGTGTC CCGCATGTCC
    AACTCTCACA CCTCGTGTAC CTCCAGTGCC TCACCATCCA GTAATGAAAG CAGTATGAAG
    GACGAGGAGG AGCAGATGTG TCCCATCTGT CTGCTGGACA TGCTGGATGA AGAAAGTCTG
    ACAGTGTGTG AAGAGGGCTG CCGAAACAAA CTCCACCATC ACTGCATGTC TATATGGGCC
    GAGGAGTGCA GAAGAAATCA CGATCCTTTA ATCTGCCCAC TGTGTAGAGC CAAATGGAAG
    CCTCATGATT TTTACAGTTA TGAAGCGCCA TCTACTGTGG AAAGCACAAC CAGCCGCTCT
    CAGAGTCAAA CAGCTGCTTC ATCATCATCA TCATCTTCAT CTCTTCATGC TGAGAGCTCC
    AGGACGGGCC AGGATGGAGA CTTCTCTCTT CCTCAATATG GAGTTCAGCA CATCCCTCAG
    ACGTACACGG AGCTCGCCGA ACCCTGGATC AAGGTGTTTG GATTGGAGCT GGTCGGCTGC
    CTCTTCTCTC GTAACTGGAA CATCAGAGAA ATGGCTCTGC GGCGGCTGTC CCATGACGTG
    AGTGGAGCCC TGCTCTTGGC CAACGGCGAG CACTCATTGG CCGGAGCGGG TGCAGGGGCA
    GGGCTGGCCG AGGTATCTGG AGACGTGGTG GTGGAGTCCT GCTGTAGTGT GCTCTCCATG
    GTCTGTGCAG ACCCTGTCTA CAAAGTCTAC GTAGCAGCGC TGAAAACCCT GCGAGCGATG
    CTTGTCTACA CCCCATGCCA CTCTGCTTCA GAGAGGACTC GATTACAGCA GCTTCTGCGG
    CCCGTGGTGG AAACCATACT GGTCAAGTGT GCAGATGCTA ACAGTCGCAC CAGTCAGCTG
    TCCGTGTCCA CTCTGCTGGA GCTGTGCAAA GGTCAAATGG GAGAACTCGC TGTGGGCAGA
    GAAATTCTCA AAGCAGGCTC TATTGGTATT GGTGGAGTTG ACTATGTCCT GAACTGCATC
    TTGGGCTCTA AAACGGAGGC CAATAACTGG CAAGCACTGC TGGGTCGGCT GTGTCTGATT
    GACAGGCTCT TACTGGAGTT TCCTGCTGAA TTCTACCCTC ACATCGTGTC AGGTGGAGAC
    TGCAACCAGG CGCAAAACGT TGTGGAGAGG TACCAGAAGC TGCTGCCTCT TCTAAGTTTT
    GCTCTGAAGT CCATTGATAA CTCTCACTCC ATGGTGGGAA AGCTTTCTCG TCGTGTCTTC
    CTGAGCGCTG CCCGTATGGT AGCTCGTGTG CCTCATGTCT TCGTTAAGCT ACTGGACATG
    CTCAACGTCA CCAGCTCCAC CCACTATGCA CGCATGCGCC GGCGTCTTCT GGCCATTGCG
    GAGGAGATGG ACATCCTGGA TGCGATTCAT CTGGGACTAG ATGACCTACA GAAAGAGCAC
    ACCTCACCCT CTTGTGCTTT CCTTGAACCC TCAGCCCCTC ATAACTCCCC ATGTACGACT
    GAAAGAAGCT CCCCCACCCA TGGTCTACAG CAGCCCGCCG CTAAGGTGGC ACTTTCAGGT
    CAAGCCAGTT CTACTCCTGT AGAGGACATA ACCGAGAGAC TCTCAAACAT AGTCGCTAGC
    CCTGCTGAAT TTCCAAAGCC CCCCATTCAG GCTCGAAACA GACCCGTTAG TCAGTGTCTG
    AACTCCCCCT CATCTGGTCA ACCCCCAAGC CTTCCTTCTC TGTCCATGGC CAATGCCTCA
    GCCTTCCCTC CAGAAAACCC CAAAGCCCTG CCACAGAGTT TTATTCCCAG CAAGTTTGCC
    CAGTCCTCAC TGGGTTCCCA ACGCAAGTTG CCTCTTCAGA AAGGTGCCTG TAGTAGTAAA
    GAACCAGAGA AGCTTCCATC CCCTGTGTTT ACTCAAGCTC GGCCACTGCC CCAGAGTCAG
    ATCCACCGGC CCAGACCTTC TCGACCCACT CCTGCTGCCT CATCTGATGG AGCTAAAGCT
    TTAAGCCCTT TGACAAAGGG CAACATGAAA CTGGACCTTC AGGAGGCCCG GTCCAGAGAT
    GATGTGGCCT GCAGTGGGGG ATCTGCTCTC ATCCCCAGTG AGGACACTGC CTTCACGCCA
    GTGGAGGAGA AGTTCCACGG TCTGGATGCT TCAGCCGATC TCAGCTCCAG CATGGAGGAC
    CTGCTGGAAG CCTCTATCCC TGCGGCAGAC AGCACGGTCA CCTTCCAGTC TGAAGTGGCA
    GTGCTGTCCC CCGAGCAGGC AAATGCAAAC GATGACACGT ACAGTGAAGA CGTGACCCAG
    AACCAGAAGT GCAAGGAGAA GATGGAAGCT GAAGAAGAGG AGGCATTGGC TGTAGTCATG
    GCCATGTCTG AGTCACAAGA CGCCCTACCT GTAATCCCAC AGTTACAGGT GGACAAAGGG
    GAGGACGTGA TCATTATTCA GGTGGATACA CCAGAGACTC TGCCTGGTCA TACCAAAGCA
    AAGCAGCCAT ACAGGGAGGG AAACGAATGG CTAAAAGGTC AACAGATTGG CCTGGGTGCC
    TTTTCGTCAT GTTACCAGGC TCAGGATGTG GGCACTGGGA CTCTGATGGC TGTCAAACAG
    GTGACGTACG TGAGGAACAC TTCATCAGAG CAGGAGGAGG TGGTTGAGGC GCTACGAGAG
    GAGATTCGCA TGATGGGTCT ACTAAACCAC CCACACATCA TCCGCATGCT GGGGGCAACC
    TGTGAGAAGA ACAACTACAA CCTTTTCGTA GAGTGGATGG CAGGTGGCTC TGTATCTCAT
    CTCTTGAATA AGTATGGCGC ATTTAAAGAA GGTGTGATCA TCAACTACAC GGAGCAGCTT
    CTGCGGGGTC TGGCCTACCT TCATGAGAAC CAGATCATCC ACCGCGACAT CAAAGGTGCC
    AATCTACTGA TTGACAGCAC AGGCCAGAGG CTGAGGATAG CTGATTTTGG GGCTGCAGCA
    CGTTTGGCTT CGAAAGGCAC TGGAGCAGGG GAGTTCCAGG GTCAACTTCT GGGAACCATT
    GCCTTCATGG CTCCGGAGGT GCTGAGAGGA CAGCAGTACG GCCGGAGCTG TGATGTCTGG
    AGTGTTGGCT GTGCCATCAT TGAGATGTCC TGTGCTAAAC CTCCCTGGAA CGCTGAAAAA
    CACTCCAACC ACCTGGCGCT CATTTTCAAG ATCGCCAGCG CCACCACAGC GCCCTCCATC
    CCACCACACT TGTCTCCAGG CCTGCGGGAC GTGACCCTGC GCTGTCTGGA GCTCCAGCCC
    ACCGACCGGC CGCCCTCCAG AGAGCTCCTG AAACACCCCG TCTTCAGACA CAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa49967
    Clone ID Related Accession (Same CDS sequence) XM_009305108.2
    Accession Version XM_009305108.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4005bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product mitogen-activated protein kinase kinase kinase 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305108.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGAACA AGGAGACTCT GCGCGGGCTG CAGAAGATGG AGGACCGGCC TGAGGAGCGA 
    CTGATCCGAG AGAAGCTCAA GGCCACCTGT ATGCCCACCT GGAAGAGCGA GTGGCTGGAG
    AGGCGGAGCA GGAGAGGACC AATGGTTGTG AAGCCCATTC CTCTGAGAGC CGACGGCACT
    GAAGCCGGGA GTGACAGTCA CAACTCTTCT TCATCCCAGA GCCGAGGTCA ACGCAGCCCG
    TCTCCCGGAC CTTCCTCTTT CTCAGCCGCT TCCTCCTCCT CATCATCGTC CTCCACAAAG
    ACTGCTGGGA AGCCAGATTC TCCTGGCCTA CGCAGACGGA GAGCTTCACC AGTGCCCAGT
    GGACGTGTGA CCCCTCCTCG AAGAGCTCCC TCCCCTGATG GCTTTTCCCC ATACAGTCCA
    GAGGAGACCA GCCGCAGGGT CAACAAGGTC ATGAGGGCGC GCCTGTACCT GCTCCAGCAA
    ATAGGACCCA ACTCATTCTT GATTGGCGGA GACAGTCCTG ACAACAAGTT CAGGGTGTTC
    ATTGGTCCAC AGACCTGCAG CTGCGGCAGA GGAACTTTCT GCATCCATGT GCTCTTCGTC
    ATGCTCCGAG TTTTCCAGCT GGAGCCTTCA GATCCTCTTC TGTGGCGGAA AACCCTCAAG
    AATTTTGAGG TGGAGAGCTT ATTTCAGAAG TACCACAATC GCCGCAGCTC CCGCATCAAA
    GCTCCCTCAC GCAGCACCAT CCAGAAGTTT GTGTCCCGCA TGTCCAACTC TCACACCTCG
    TGTACCTCCA GTGCCTCACC ATCCAGTAAT GAAAGCAGTA TGAAGGACGA GGAGGAGCAG
    ATGTGTCCCA TCTGTCTGCT GGACATGCTG GATGAAGAAA GTCTGACAGT GTGTGAAGAG
    GGCTGCCGAA ACAAACTCCA CCATCACTGC ATGTCTATAT GGGCCGAGGA GTGCAGAAGA
    AATCACGATC CTTTAATCTG CCCACTGTGT AGAGCCAAAT GGAAGCCTCA TGATTTTTAC
    AGTTATGAAG CGCCATCTAC TGTGGAAAGC ACAACCAGCC GCTCTCAGAG TCAAACAGCT
    GCTTCATCAT CATCATCATC TTCATCTCTT CATGCTGAGA GCTCCAGGAC GGGCCAGGAT
    GGAGACTTCT CTCTTCCTCA ATATGGAGTT CAGCACATCC CTCAGACGTA CACGGAGCTC
    GCCGAACCCT GGATCAAGGT GTTTGGATTG GAGCTGGTCG GCTGCCTCTT CTCTCGTAAC
    TGGAACATCA GAGAAATGGC TCTGCGGCGG CTGTCCCATG ACGTGAGTGG AGCCCTGCTC
    TTGGCCAACG GCGAGCACTC ATTGGCCGGA GCGGGTGCAG GGGCAGGGCT GGCCGAGGTA
    TCTGGAGACG TGGTGGTGGA GTCCTGCTGT AGTGTGCTCT CCATGGTCTG TGCAGACCCT
    GTCTACAAAG TCTACGTAGC AGCGCTGAAA ACCCTGCGAG CGATGCTTGT CTACACCCCA
    TGCCACTCTG CTTCAGAGAG GACTCGATTA CAGCAGCTTC TGCGGCCCGT GGTGGAAACC
    ATACTGGTCA AGTGTGCAGA TGCTAACAGT CGCACCAGTC AGCTGTCCGT GTCCACTCTG
    CTGGAGCTGT GCAAAGGTCA AATGGGAGAA CTCGCTGTGG GCAGAGAAAT TCTCAAAGCA
    GGCTCTATTG GTATTGGTGG AGTTGACTAT GTCCTGAACT GCATCTTGGG CTCTAAAACG
    GAGGCCAATA ACTGGCAAGC ACTGCTGGGT CGGCTGTGTC TGATTGACAG GCTCTTACTG
    GAGTTTCCTG CTGAATTCTA CCCTCACATC GTGTCAGGTG GAGACTGCAA CCAGGCGCAA
    AACGTTGTGG AGAGGTACCA GAAGCTGCTG CCTCTTCTAA GTTTTGCTCT GAAGTCCATT
    GATAACTCTC ACTCCATGGT GGGAAAGCTT TCTCGTCGTG TCTTCCTGAG CGCTGCCCGT
    ATGGTAGCTC GTGTGCCTCA TGTCTTCGTT AAGCTACTGG ACATGCTCAA CGTCACCAGC
    TCCACCCACT ATGCACGCAT GCGCCGGCGT CTTCTGGCCA TTGCGGAGGA GATGGACATC
    CTGGATGCGA TTCATCTGGG ACTAGATGAC CTACAGAAAG AGCACACCTC ACCCTCTTGT
    GCTTTCCTTG AACCCTCAGC CCCTCATAAC TCCCCATGTA CGACTGAAAG AAGCTCCCCC
    ACCCATGGTC TACAGCAGCC CGCCGCTAAG GTGGCACTTT CAGGTCAAGC CAGTTCTACT
    CCTGTAGAGG ACATAACCGA GAGACTCTCA AACATAGTCG CTAGCCCTGC TGAATTTCCA
    AAGCCCCCCA TTCAGGCTCG AAACAGACCC GTTAGTCAGT GTCTGAACTC CCCCTCATCT
    GGTCAACCCC CAAGCCTTCC TTCTCTGTCC ATGGCCAATG CCTCAGCCTT CCCTCCAGAA
    AACCCCAAAG CCCTGCCACA GAGTTTTATT CCCAGCAAGT TTGCCCAGTC CTCACTGGGT
    TCCCAACGCA AGTTGCCTCT TCAGAAAGGT GCCTGTAGTA GTAAAGAACC AGAGAAGCTT
    CCATCCCCTG TGTTTACTCA AGCTCGGCCA CTGCCCCAGA GTCAGATCCA CCGGCCCAGA
    CCTTCTCGAC CCACTCCTGC TGCCTCATCT GATGGAGCTA AAGCTTTAAG CCCTTTGACA
    AAGGGCAACA TGAAACTGGA CCTTCAGGAG GCCCGGTCCA GAGATGATGT GGCCTGCAGT
    GGGGGATCTG CTCTCATCCC CAGTGAGGAC ACTGCCTTCA CGCCAGTGGA GGAGAAGTTC
    CACGGTCTGG ATGCTTCAGC CGATCTCAGC TCCAGCATGG AGGACCTGCT GGAAGCCTCT
    ATCCCTGCGG CAGACAGCAC GGTCACCTTC CAGTCTGAAG TGGCAGTGCT GTCCCCCGAG
    CAGGCAAATG CAAACGATGA CACGTACAGT GAAGACGTGA CCCAGAACCA GAAGTGCAAG
    GAGAAGATGG AAGCTGAAGA AGAGGAGGCA TTGGCTGTAG TCATGGCCAT GTCTGAGTCA
    CAAGACGCCC TACCTGTAAT CCCACAGTTA CAGGTGGACA AAGGGGAGGA CGTGATCATT
    ATTCAGGTGG ATACACCAGA GACTCTGCCT GGTCATACCA AAGCAAAGCA GCCATACAGG
    GAGGGAAACG AATGGCTAAA AGGTCAACAG ATTGGCCTGG GTGCCTTTTC GTCATGTTAC
    CAGGCTCAGG ATGTGGGCAC TGGGACTCTG ATGGCTGTCA AACAGGTGAC GTACGTGAGG
    AACACTTCAT CAGAGCAGGA GGAGGTGGTT GAGGCGCTAC GAGAGGAGAT TCGCATGATG
    GGTCTACTAA ACCACCCACA CATCATCCGC ATGCTGGGGG CAACCTGTGA GAAGAACAAC
    TACAACCTTT TCGTAGAGTG GATGGCAGGT GGCTCTGTAT CTCATCTCTT GAATAAGTAT
    GGCGCATTTA AAGAAGGTGT GATCATCAAC TACACGGAGC AGCTTCTGCG GGGTCTGGCC
    TACCTTCATG AGAACCAGAT CATCCACCGC GACATCAAAG GTGCCAATCT ACTGATTGAC
    AGCACAGGCC AGAGGCTGAG GATAGCTGAT TTTGGGGCTG CAGCACGTTT GGCTTCGAAA
    GGCACTGGAG CAGGGGAGTT CCAGGGTCAA CTTCTGGGAA CCATTGCCTT CATGGCTCCG
    GAGGTGCTGA GAGGACAGCA GTACGGCCGG AGCTGTGATG TCTGGAGTGT TGGCTGTGCC
    ATCATTGAGA TGTCCTGTGC TAAACCTCCC TGGAACGCTG AAAAACACTC CAACCACCTG
    GCGCTCATTT TCAAGATCGC CAGCGCCACC ACAGCGCCCT CCATCCCACC ACACTTGTCT
    CCAGGCCTGC GGGACGTGAC CCTGCGCTGT CTGGAGCTCC AGCCCACCGA CCGGCCGCCC
    TCCAGAGAGC TCCTGAAACA CCCCGTCTTC AGACACAGCT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009303383.1
    CDS108..4112
    Translation

    Target ORF information:

    RefSeq Version XM_009305108.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (map3k1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009305108.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGAACA AGGAGACTCT GCGCGGGCTG CAGAAGATGG AGGACCGGCC TGAGGAGCGA 
    CTGATCCGAG AGAAGCTCAA GGCCACCTGT ATGCCCACCT GGAAGAGCGA GTGGCTGGAG
    AGGCGGAGCA GGAGAGGACC AATGGTTGTG AAGCCCATTC CTCTGAGAGC CGACGGCACT
    GAAGCCGGGA GTGACAGTCA CAACTCTTCT TCATCCCAGA GCCGAGGTCA ACGCAGCCCG
    TCTCCCGGAC CTTCCTCTTT CTCAGCCGCT TCCTCCTCCT CATCATCGTC CTCCACAAAG
    ACTGCTGGGA AGCCAGATTC TCCTGGCCTA CGCAGACGGA GAGCTTCACC AGTGCCCAGT
    GGACGTGTGA CCCCTCCTCG AAGAGCTCCC TCCCCTGATG GCTTTTCCCC ATACAGTCCA
    GAGGAGACCA GCCGCAGGGT CAACAAGGTC ATGAGGGCGC GCCTGTACCT GCTCCAGCAA
    ATAGGACCCA ACTCATTCTT GATTGGCGGA GACAGTCCTG ACAACAAGTT CAGGGTGTTC
    ATTGGTCCAC AGACCTGCAG CTGCGGCAGA GGAACTTTCT GCATCCATGT GCTCTTCGTC
    ATGCTCCGAG TTTTCCAGCT GGAGCCTTCA GATCCTCTTC TGTGGCGGAA AACCCTCAAG
    AATTTTGAGG TGGAGAGCTT ATTTCAGAAG TACCACAATC GCCGCAGCTC CCGCATCAAA
    GCTCCCTCAC GCAGCACCAT CCAGAAGTTT GTGTCCCGCA TGTCCAACTC TCACACCTCG
    TGTACCTCCA GTGCCTCACC ATCCAGTAAT GAAAGCAGTA TGAAGGACGA GGAGGAGCAG
    ATGTGTCCCA TCTGTCTGCT GGACATGCTG GATGAAGAAA GTCTGACAGT GTGTGAAGAG
    GGCTGCCGAA ACAAACTCCA CCATCACTGC ATGTCTATAT GGGCCGAGGA GTGCAGAAGA
    AATCACGATC CTTTAATCTG CCCACTGTGT AGAGCCAAAT GGAAGCCTCA TGATTTTTAC
    AGTTATGAAG CGCCATCTAC TGTGGAAAGC ACAACCAGCC GCTCTCAGAG TCAAACAGCT
    GCTTCATCAT CATCATCATC TTCATCTCTT CATGCTGAGA GCTCCAGGAC GGGCCAGGAT
    GGAGACTTCT CTCTTCCTCA ATATGGAGTT CAGCACATCC CTCAGACGTA CACGGAGCTC
    GCCGAACCCT GGATCAAGGT GTTTGGATTG GAGCTGGTCG GCTGCCTCTT CTCTCGTAAC
    TGGAACATCA GAGAAATGGC TCTGCGGCGG CTGTCCCATG ACGTGAGTGG AGCCCTGCTC
    TTGGCCAACG GCGAGCACTC ATTGGCCGGA GCGGGTGCAG GGGCAGGGCT GGCCGAGGTA
    TCTGGAGACG TGGTGGTGGA GTCCTGCTGT AGTGTGCTCT CCATGGTCTG TGCAGACCCT
    GTCTACAAAG TCTACGTAGC AGCGCTGAAA ACCCTGCGAG CGATGCTTGT CTACACCCCA
    TGCCACTCTG CTTCAGAGAG GACTCGATTA CAGCAGCTTC TGCGGCCCGT GGTGGAAACC
    ATACTGGTCA AGTGTGCAGA TGCTAACAGT CGCACCAGTC AGCTGTCCGT GTCCACTCTG
    CTGGAGCTGT GCAAAGGTCA AATGGGAGAA CTCGCTGTGG GCAGAGAAAT TCTCAAAGCA
    GGCTCTATTG GTATTGGTGG AGTTGACTAT GTCCTGAACT GCATCTTGGG CTCTAAAACG
    GAGGCCAATA ACTGGCAAGC ACTGCTGGGT CGGCTGTGTC TGATTGACAG GCTCTTACTG
    GAGTTTCCTG CTGAATTCTA CCCTCACATC GTGTCAGGTG GAGACTGCAA CCAGGCGCAA
    AACGTTGTGG AGAGGTACCA GAAGCTGCTG CCTCTTCTAA GTTTTGCTCT GAAGTCCATT
    GATAACTCTC ACTCCATGGT GGGAAAGCTT TCTCGTCGTG TCTTCCTGAG CGCTGCCCGT
    ATGGTAGCTC GTGTGCCTCA TGTCTTCGTT AAGCTACTGG ACATGCTCAA CGTCACCAGC
    TCCACCCACT ATGCACGCAT GCGCCGGCGT CTTCTGGCCA TTGCGGAGGA GATGGACATC
    CTGGATGCGA TTCATCTGGG ACTAGATGAC CTACAGAAAG AGCACACCTC ACCCTCTTGT
    GCTTTCCTTG AACCCTCAGC CCCTCATAAC TCCCCATGTA CGACTGAAAG AAGCTCCCCC
    ACCCATGGTC TACAGCAGCC CGCCGCTAAG GTGGCACTTT CAGGTCAAGC CAGTTCTACT
    CCTGTAGAGG ACATAACCGA GAGACTCTCA AACATAGTCG CTAGCCCTGC TGAATTTCCA
    AAGCCCCCCA TTCAGGCTCG AAACAGACCC GTTAGTCAGT GTCTGAACTC CCCCTCATCT
    GGTCAACCCC CAAGCCTTCC TTCTCTGTCC ATGGCCAATG CCTCAGCCTT CCCTCCAGAA
    AACCCCAAAG CCCTGCCACA GAGTTTTATT CCCAGCAAGT TTGCCCAGTC CTCACTGGGT
    TCCCAACGCA AGTTGCCTCT TCAGAAAGGT GCCTGTAGTA GTAAAGAACC AGAGAAGCTT
    CCATCCCCTG TGTTTACTCA AGCTCGGCCA CTGCCCCAGA GTCAGATCCA CCGGCCCAGA
    CCTTCTCGAC CCACTCCTGC TGCCTCATCT GATGGAGCTA AAGCTTTAAG CCCTTTGACA
    AAGGGCAACA TGAAACTGGA CCTTCAGGAG GCCCGGTCCA GAGATGATGT GGCCTGCAGT
    GGGGGATCTG CTCTCATCCC CAGTGAGGAC ACTGCCTTCA CGCCAGTGGA GGAGAAGTTC
    CACGGTCTGG ATGCTTCAGC CGATCTCAGC TCCAGCATGG AGGACCTGCT GGAAGCCTCT
    ATCCCTGCGG CAGACAGCAC GGTCACCTTC CAGTCTGAAG TGGCAGTGCT GTCCCCCGAG
    CAGGCAAATG CAAACGATGA CACGTACAGT GAAGACGTGA CCCAGAACCA GAAGTGCAAG
    GAGAAGATGG AAGCTGAAGA AGAGGAGGCA TTGGCTGTAG TCATGGCCAT GTCTGAGTCA
    CAAGACGCCC TACCTGTAAT CCCACAGTTA CAGGTGGACA AAGGGGAGGA CGTGATCATT
    ATTCAGGTGG ATACACCAGA GACTCTGCCT GGTCATACCA AAGCAAAGCA GCCATACAGG
    GAGGGAAACG AATGGCTAAA AGGTCAACAG ATTGGCCTGG GTGCCTTTTC GTCATGTTAC
    CAGGCTCAGG ATGTGGGCAC TGGGACTCTG ATGGCTGTCA AACAGGTGAC GTACGTGAGG
    AACACTTCAT CAGAGCAGGA GGAGGTGGTT GAGGCGCTAC GAGAGGAGAT TCGCATGATG
    GGTCTACTAA ACCACCCACA CATCATCCGC ATGCTGGGGG CAACCTGTGA GAAGAACAAC
    TACAACCTTT TCGTAGAGTG GATGGCAGGT GGCTCTGTAT CTCATCTCTT GAATAAGTAT
    GGCGCATTTA AAGAAGGTGT GATCATCAAC TACACGGAGC AGCTTCTGCG GGGTCTGGCC
    TACCTTCATG AGAACCAGAT CATCCACCGC GACATCAAAG GTGCCAATCT ACTGATTGAC
    AGCACAGGCC AGAGGCTGAG GATAGCTGAT TTTGGGGCTG CAGCACGTTT GGCTTCGAAA
    GGCACTGGAG CAGGGGAGTT CCAGGGTCAA CTTCTGGGAA CCATTGCCTT CATGGCTCCG
    GAGGTGCTGA GAGGACAGCA GTACGGCCGG AGCTGTGATG TCTGGAGTGT TGGCTGTGCC
    ATCATTGAGA TGTCCTGTGC TAAACCTCCC TGGAACGCTG AAAAACACTC CAACCACCTG
    GCGCTCATTT TCAAGATCGC CAGCGCCACC ACAGCGCCCT CCATCCCACC ACACTTGTCT
    CCAGGCCTGC GGGACGTGAC CCTGCGCTGT CTGGAGCTCC AGCCCACCGA CCGGCCGCCC
    TCCAGAGAGC TCCTGAAACA CCCCGTCTTC AGACACAGCT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.