tiam2a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tiam2a
Entrez Gene ID	100334442
Full Name	T cell lymphoma invasion and metastasis 2a
Synonyms	id:ibd5033,tiam2
General protein information	Preferred Names T cell lymphoma invasion and metastasis 2a Names T-lymphoma invasion and metastasis-inducing protein 2 T-cell lymphoma invasion and metastasis 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009293289.2	XP_009291564.1	T-lymphoma invasion and metastasis-inducing protein 2 isoform X2
XM_009293290.2	XP_009291565.1	T-lymphoma invasion and metastasis-inducing protein 2 isoform X3
XM_021467480.1	XP_021323155.1	T-lymphoma invasion and metastasis-inducing protein 2 isoform X1
XM_009293288.2	XP_009291563.1	T-lymphoma invasion and metastasis-inducing protein 2 isoform X1
XM_009293290.3	XP_009291565.1	T-lymphoma invasion and metastasis-inducing protein 2 isoform X3
XM_009293288.3	XP_009291563.1	T-lymphoma invasion and metastasis-inducing protein 2 isoform X1
XM_009293289.3	XP_009291564.1	T-lymphoma invasion and metastasis-inducing protein 2 isoform X2
XM_021467481.1	XP_021323156.1	T-lymphoma invasion and metastasis-inducing protein 2 isoform X4

Pathways from BioSystems

Homologies

Homo sapiens (human)	TIAM2	NP_036586.2
	LOC701688	XP_002808441.1
Canis lupus familiaris (dog)	TIAM2	XP_541162.4
Bos taurus (cattle)	TIAM2	XP_003586537.2
Mus musculus (house mouse)	Tiam2	NP_036008.2
Rattus norvegicus (Norway rat)	LOC100362710	XP_003748741.1
Gallus gallus (chicken)	TIAM2	NP_001254464.1
Pan troglodytes (chimpanzee)	TIAM2	XP_527545.2
Danio rerio (zebrafish)	tiam2	XP_002665298.2

Gene Ontology

The following tiam2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tiam2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa49966	XM_009293290.3 Latest version!	Danio rerio T-cell lymphoma invasion and metastasis 2a (tiam2a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa149763	XM_021467481.1 Latest version!	Danio rerio T-cell lymphoma invasion and metastasis 2a (tiam2a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa49964	XM_009293288.3 Latest version!	Danio rerio T-cell lymphoma invasion and metastasis 2a (tiam2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa49964	XM_021467480.1 Latest version!	Danio rerio T-cell lymphoma invasion and metastasis 2a (tiam2a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa49965	XM_009293289.3 Latest version!	Danio rerio T-cell lymphoma invasion and metastasis 2a (tiam2a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
ODa49966	XM_009293290.2	Danio rerio T-cell lymphoma invasion and metastasis 2a (tiam2a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa49964	XM_009293288.2	Danio rerio T-cell lymphoma invasion and metastasis 2a (tiam2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa49965	XM_009293289.2	Danio rerio T-cell lymphoma invasion and metastasis 2a (tiam2a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tiam2a ( XM_009293290.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009291565.1 Danio rerio T-cell lymphoma invasion and metastasis 2a (tiam2a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa49966
Clone ID Related Accession (Same CDS sequence)	XM_009293290.2 , XM_009293290.3
Accession Version	XM_009293290.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	T-lymphoma invasion and metastasis-inducing protein 2 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293290.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC AGACCGAACG GGATGCCAGG CCATGCATCT ACCTCACGGC GCTCATCGAG 
CTCTGCCTCT GCGAGCACTC GCCCGTCGAG CTTCTCTGTT CCTTCTTTAC CATGTGCTGG 
CTCTGGCCAG ACTGTGTGAA GGAAGGCAAG GATATCTTCC TCAACTCGGC TGTGCTGACT 
CTCCACCGGC TACTCCAGAA CAAGCTGGCC CGACTGCTAG ATGAGATCGC CGCCTGCGTG 
GCCTGCCAGA ATGGTGCCGA AGGATCCATT TTCATGAGTG TGGACACGGT GTGTTCGCTG 
TACCACTCCT ACCAGGAAGG CTCTGGAGGT CTGATGGAGA GCCAGGAGGA GCAAACGGGG 
CCTGAACCCG CTATCCTCAG GCCCTGTCCG CGTCATATGT CTGCCACAGA GAGACTACGC 
AAGGTCATCC AGGAATTAGT GGATACAGAG AAATCCTACG TAAAGGACCT CAACTGCTTG 
TTTGAAATCT ACCTGAAGCC CCTGCAAAAG GAGACCTTTC TCACTCAAGA TGAGATGGAA 
AGCCTATTTG GCAGCCTTCC AGAGATGCTG GACTTCCAGA AAGTTTTCCT GCAGACCCTG 
GAAGAGAAGA TTAGTTCCTC CCCCAACTTC AACACGTTCG AGACCCCTGT CCAATTCAAG 
AAGCTGCTCT TCTCTCTGGG TGGCTCATTT CTGTATTATG CGGAGCACTT CAAACTGTAC 
AGCGGCTTCT GCGCCAACCA TATTAAAGTG CAGAAGGTTC TGGAACGAGC TAAAACAGAT 
CGATCTTTTA AAGAGTTCCT CGATGCTAGG AACCCGACCA AGCAGCACTC AACCACGCTA 
GAGTCCTACC TGATCAAACC AGTGCAGAGA GTGCTGAAAT ACCCCCTGCT GCTCCGCGAG 
CTGGTGTCTC TGACGGACAC GGACAGTGAG GAGCACTACC ACCTCACAGA GGCGCTTAAG 
GAAATGGAAA AGGTCGCAAG CCATATAAAC GAAATGCAGA AGATCTACGA AGACTACGGT 
GCAGTGTTTG ACCAGCTGGT GGCAGAGCAA AGTGGCACAG AGAAAGAGGT CACAGAGATA 
TCTATGGGAC AGTTCCTCAT GCATTCCTCT GTGATCTGGC TCAACCCGTT CCCATCGTTG 
GGCCGTATGA AAAAGGATCC CGAGCTGACT GTTTTTGTTT TTAAGAGAGC CGTGATACTG 
CTGTACCGGG AGAACAACAA GGTGAGGAAG AAGATGGTGA CTTCAAGATC AGCCCACGGA 
GATCAGGATT CATTCAAGTT TCGCTGGCTC ATTCCCCTGT CAGCGCTACA GGTCAGACTG 
GGCAACACTG CAGACACAGA CTGCCTCTGG GAGCTGATTC ACACCAAATC ACAAAAGGAG 
GGCAGGCCTG AGACGGTGTT CCAGTTGTGC AGCAGCACAC CAGAGTGCAA AGTGAATATC 
ATCAAGGTGA TGCGCTCCAT CTTGCGGGAG AACCTGCGTC GAAACATGCA AAGGGTTGAA 
CAGTCTGAGA GAACCTGTAA AAAGCGGCTT ACTCCCCTTC GGAAAAGCCT GCCAGCTTCG 
GCTAGGATAG GTTCTTCTCG AGCCTTTTGG CTAGCCAAAC AACCTTCAAA CGACAGTCCC 
GTTCCCAGCA ATGATCCACT GAGGCTGTCT CTAGCAGATT CTGATTCAGC CAGTGCTAGC 
AGTGGAACCA CCAGCAGTGC CAGCGCTCCT CTAGATCCGC CATCACAGCA GCAGAGCGGC 
CTCAGAGAAT CCAGCATAAT CAGCGATGAG GACGAAGACG GCAGCGAGCC GGCTGATTGG 
ACGGATGCTC CCAATGCTAT CGAGGTCCAA TTTCGCAAGC TTGGTCTGAC CGAGGAGACC 
TCCCAGGCAG CTGCTCCACG TGTGCAGCCG GAGAGCGGAG AAGTGGACAC TCCAGAAAGG 
AAGGCTTCTC TAAAGCGAGC TCACTTTGCT GTGGTCAAAC GCAAGCCCGC CAGAGACACG 
AATAGCCTCA GACGCAGTCA GGTGGCCCTG CTGAACATGC GTAGACACAG CCACTCTTTG 
GACATCCCAG CGGAGATCGC GGCACATAGT GTAGACCTGA ACTCGCTACT GGAGAAAGAT 
TTCAGCGTGC AGAGCCTGAC CTCTGTTGTC AACGAAGACT TCTTCTATGA CAATCCCATG 
GAGCCAAAGA ATGTGACAAC TTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291565.1
CDS	172..2358
Translation	MAEQTERDARPCIYLTALIELCLCEHSPVELLCSFFTMCWLWPDCVKEGKDIFLNSAVLTLHRLLQNKLARLLDEIAACVACQNGAEGSIFMSVDTVCSLYHSYQEGSGGLMESQEEQTGPEPAILRPCPRHMSATERLRKVIQELVDTEKSYVKDLNCLFEIYLKPLQKETFLTQDEMESLFGSLPEMLDFQKVFLQTLEEKISSSPNFNTFETPVQFKKLLFSLGGSFLYYAEHFKLYSGFCANHIKVQKVLERAKTDRSFKEFLDARNPTKQHSTTLESYLIKPVQRVLKYPLLLRELVSLTDTDSEEHYHLTEALKEMEKVASHINEMQKIYEDYGAVFDQLVAEQSGTEKEVTEISMGQFLMHSSVIWLNPFPSLGRMKKDPELTVFVFKRAVILLYRENNKVRKKMVTSRSAHGDQDSFKFRWLIPLSALQVRLGNTADTDCLWELIHTKSQKEGRPETVFQLCSSTPECKVNIIKVMRSILRENLRRNMQRVEQSERTCKKRLTPLRKSLPASARIGSSRAFWLAKQPSNDSPVPSNDPLRLSLADSDSASASSGTTSSASAPLDPPSQQQSGLRESSIISDEDEDGSEPADWTDAPNAIEVQFRKLGLTEETSQAAAPRVQPESGEVDTPERKASLKRAHFAVVKRKPARDTNSLRRSQVALLNMRRHSHSLDIPAEIAAHSVDLNSLLEKDFSVQSLTSVVNEDFFYDNPMEPKNVTTS

Target ORF information:

RefSeq Version	XM_009293290.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio T-cell lymphoma invasion and metastasis 2a (tiam2a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293290.2

ORF Insert Sequence:

ATGGCAGAGC AGACCGAACG GGATGCCAGG CCATGCATCT ACCTCACGGC GCTCATCGAG 
CTCTGCCTCT GCGAGCACTC GCCCGTCGAG CTTCTCTGTT CCTTCTTTAC CATGTGCTGG 
CTCTGGCCAG ACTGTGTGAA GGAAGGCAAG GATATCTTCC TCAACTCGGC TGTGCTGACT 
CTCCACCGGC TACTCCAGAA CAAGCTGGCC CGACTGCTAG ATGAGATCGC CGCCTGCGTG 
GCCTGCCAGA ATGGTGCCGA AGGATCCATT TTCATGAGTG TGGACACGGT GTGTTCGCTG 
TACCACTCCT ACCAGGAAGG CTCTGGAGGT CTGATGGAGA GCCAGGAGGA GCAAACGGGG 
CCTGAACCCG CTATCCTCAG GCCCTGTCCG CGTCATATGT CTGCCACAGA GAGACTACGC 
AAGGTCATCC AGGAATTAGT GGATACAGAG AAATCCTACG TAAAGGACCT CAACTGCTTG 
TTTGAAATCT ACCTGAAGCC CCTGCAAAAG GAGACCTTTC TCACTCAAGA TGAGATGGAA 
AGCCTATTTG GCAGCCTTCC AGAGATGCTG GACTTCCAGA AAGTTTTCCT GCAGACCCTG 
GAAGAGAAGA TTAGTTCCTC CCCCAACTTC AACACGTTCG AGACCCCTGT CCAATTCAAG 
AAGCTGCTCT TCTCTCTGGG TGGCTCATTT CTGTATTATG CGGAGCACTT CAAACTGTAC 
AGCGGCTTCT GCGCCAACCA TATTAAAGTG CAGAAGGTTC TGGAACGAGC TAAAACAGAT 
CGATCTTTTA AAGAGTTCCT CGATGCTAGG AACCCGACCA AGCAGCACTC AACCACGCTA 
GAGTCCTACC TGATCAAACC AGTGCAGAGA GTGCTGAAAT ACCCCCTGCT GCTCCGCGAG 
CTGGTGTCTC TGACGGACAC GGACAGTGAG GAGCACTACC ACCTCACAGA GGCGCTTAAG 
GAAATGGAAA AGGTCGCAAG CCATATAAAC GAAATGCAGA AGATCTACGA AGACTACGGT 
GCAGTGTTTG ACCAGCTGGT GGCAGAGCAA AGTGGCACAG AGAAAGAGGT CACAGAGATA 
TCTATGGGAC AGTTCCTCAT GCATTCCTCT GTGATCTGGC TCAACCCGTT CCCATCGTTG 
GGCCGTATGA AAAAGGATCC CGAGCTGACT GTTTTTGTTT TTAAGAGAGC CGTGATACTG 
CTGTACCGGG AGAACAACAA GGTGAGGAAG AAGATGGTGA CTTCAAGATC AGCCCACGGA 
GATCAGGATT CATTCAAGTT TCGCTGGCTC ATTCCCCTGT CAGCGCTACA GGTCAGACTG 
GGCAACACTG CAGACACAGA CTGCCTCTGG GAGCTGATTC ACACCAAATC ACAAAAGGAG 
GGCAGGCCTG AGACGGTGTT CCAGTTGTGC AGCAGCACAC CAGAGTGCAA AGTGAATATC 
ATCAAGGTGA TGCGCTCCAT CTTGCGGGAG AACCTGCGTC GAAACATGCA AAGGGTTGAA 
CAGTCTGAGA GAACCTGTAA AAAGCGGCTT ACTCCCCTTC GGAAAAGCCT GCCAGCTTCG 
GCTAGGATAG GTTCTTCTCG AGCCTTTTGG CTAGCCAAAC AACCTTCAAA CGACAGTCCC 
GTTCCCAGCA ATGATCCACT GAGGCTGTCT CTAGCAGATT CTGATTCAGC CAGTGCTAGC 
AGTGGAACCA CCAGCAGTGC CAGCGCTCCT CTAGATCCGC CATCACAGCA GCAGAGCGGC 
CTCAGAGAAT CCAGCATAAT CAGCGATGAG GACGAAGACG GCAGCGAGCC GGCTGATTGG 
ACGGATGCTC CCAATGCTAT CGAGGTCCAA TTTCGCAAGC TTGGTCTGAC CGAGGAGACC 
TCCCAGGCAG CTGCTCCACG TGTGCAGCCG GAGAGCGGAG AAGTGGACAC TCCAGAAAGG 
AAGGCTTCTC TAAAGCGAGC TCACTTTGCT GTGGTCAAAC GCAAGCCCGC CAGAGACACG 
AATAGCCTCA GACGCAGTCA GGTGGCCCTG CTGAACATGC GTAGACACAG CCACTCTTTG 
GACATCCCAG CGGAGATCGC GGCACATAGT GTAGACCTGA ACTCGCTACT GGAGAAAGAT 
TTCAGCGTGC AGAGCCTGAC CTCTGTTGTC AACGAAGACT TCTTCTATGA CAATCCCATG 
GAGCCAAAGA ATGTGACAAC TTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tiam2a ( XM_009293290.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009291565.1 Danio rerio T-cell lymphoma invasion and metastasis 2a (tiam2a), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa49966
Clone ID Related Accession (Same CDS sequence)	XM_009293290.2 , XM_009293290.3
Accession Version	XM_009293290.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	T-lymphoma invasion and metastasis-inducing protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293290.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC AGACCGAACG GGATGCCAGG CCATGCATCT ACCTCACGGC GCTCATCGAG 
CTCTGCCTCT GCGAGCACTC GCCCGTCGAG CTTCTCTGTT CCTTCTTTAC CATGTGCTGG 
CTCTGGCCAG ACTGTGTGAA GGAAGGCAAG GATATCTTCC TCAACTCGGC TGTGCTGACT 
CTCCACCGGC TACTCCAGAA CAAGCTGGCC CGACTGCTAG ATGAGATCGC CGCCTGCGTG 
GCCTGCCAGA ATGGTGCCGA AGGATCCATT TTCATGAGTG TGGACACGGT GTGTTCGCTG 
TACCACTCCT ACCAGGAAGG CTCTGGAGGT CTGATGGAGA GCCAGGAGGA GCAAACGGGG 
CCTGAACCCG CTATCCTCAG GCCCTGTCCG CGTCATATGT CTGCCACAGA GAGACTACGC 
AAGGTCATCC AGGAATTAGT GGATACAGAG AAATCCTACG TAAAGGACCT CAACTGCTTG 
TTTGAAATCT ACCTGAAGCC CCTGCAAAAG GAGACCTTTC TCACTCAAGA TGAGATGGAA 
AGCCTATTTG GCAGCCTTCC AGAGATGCTG GACTTCCAGA AAGTTTTCCT GCAGACCCTG 
GAAGAGAAGA TTAGTTCCTC CCCCAACTTC AACACGTTCG AGACCCCTGT CCAATTCAAG 
AAGCTGCTCT TCTCTCTGGG TGGCTCATTT CTGTATTATG CGGAGCACTT CAAACTGTAC 
AGCGGCTTCT GCGCCAACCA TATTAAAGTG CAGAAGGTTC TGGAACGAGC TAAAACAGAT 
CGATCTTTTA AAGAGTTCCT CGATGCTAGG AACCCGACCA AGCAGCACTC AACCACGCTA 
GAGTCCTACC TGATCAAACC AGTGCAGAGA GTGCTGAAAT ACCCCCTGCT GCTCCGCGAG 
CTGGTGTCTC TGACGGACAC GGACAGTGAG GAGCACTACC ACCTCACAGA GGCGCTTAAG 
GAAATGGAAA AGGTCGCAAG CCATATAAAC GAAATGCAGA AGATCTACGA AGACTACGGT 
GCAGTGTTTG ACCAGCTGGT GGCAGAGCAA AGTGGCACAG AGAAAGAGGT CACAGAGATA 
TCTATGGGAC AGTTCCTCAT GCATTCCTCT GTGATCTGGC TCAACCCGTT CCCATCGTTG 
GGCCGTATGA AAAAGGATCC CGAGCTGACT GTTTTTGTTT TTAAGAGAGC CGTGATACTG 
CTGTACCGGG AGAACAACAA GGTGAGGAAG AAGATGGTGA CTTCAAGATC AGCCCACGGA 
GATCAGGATT CATTCAAGTT TCGCTGGCTC ATTCCCCTGT CAGCGCTACA GGTCAGACTG 
GGCAACACTG CAGACACAGA CTGCCTCTGG GAGCTGATTC ACACCAAATC ACAAAAGGAG 
GGCAGGCCTG AGACGGTGTT CCAGTTGTGC AGCAGCACAC CAGAGTGCAA AGTGAATATC 
ATCAAGGTGA TGCGCTCCAT CTTGCGGGAG AACCTGCGTC GAAACATGCA AAGGGTTGAA 
CAGTCTGAGA GAACCTGTAA AAAGCGGCTT ACTCCCCTTC GGAAAAGCCT GCCAGCTTCG 
GCTAGGATAG GTTCTTCTCG AGCCTTTTGG CTAGCCAAAC AACCTTCAAA CGACAGTCCC 
GTTCCCAGCA ATGATCCACT GAGGCTGTCT CTAGCAGATT CTGATTCAGC CAGTGCTAGC 
AGTGGAACCA CCAGCAGTGC CAGCGCTCCT CTAGATCCGC CATCACAGCA GCAGAGCGGC 
CTCAGAGAAT CCAGCATAAT CAGCGATGAG GACGAAGACG GCAGCGAGCC GGCTGATTGG 
ACGGATGCTC CCAATGCTAT CGAGGTCCAA TTTCGCAAGC TTGGTCTGAC CGAGGAGACC 
TCCCAGGCAG CTGCTCCACG TGTGCAGCCG GAGAGCGGAG AAGTGGACAC TCCAGAAAGG 
AAGGCTTCTC TAAAGCGAGC TCACTTTGCT GTGGTCAAAC GCAAGCCCGC CAGAGACACG 
AATAGCCTCA GACGCAGTCA GGTGGCCCTG CTGAACATGC GTAGACACAG CCACTCTTTG 
GACATCCCAG CGGAGATCGC GGCACATAGT GTAGACCTGA ACTCGCTACT GGAGAAAGAT 
TTCAGCGTGC AGAGCCTGAC CTCTGTTGTC AACGAAGACT TCTTCTATGA CAATCCCATG 
GAGCCAAAGA ATGTGACAAC TTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291565.1
CDS	158..2344
Translation	MAEQTERDARPCIYLTALIELCLCEHSPVELLCSFFTMCWLWPDCVKEGKDIFLNSAVLTLHRLLQNKLARLLDEIAACVACQNGAEGSIFMSVDTVCSLYHSYQEGSGGLMESQEEQTGPEPAILRPCPRHMSATERLRKVIQELVDTEKSYVKDLNCLFEIYLKPLQKETFLTQDEMESLFGSLPEMLDFQKVFLQTLEEKISSSPNFNTFETPVQFKKLLFSLGGSFLYYAEHFKLYSGFCANHIKVQKVLERAKTDRSFKEFLDARNPTKQHSTTLESYLIKPVQRVLKYPLLLRELVSLTDTDSEEHYHLTEALKEMEKVASHINEMQKIYEDYGAVFDQLVAEQSGTEKEVTEISMGQFLMHSSVIWLNPFPSLGRMKKDPELTVFVFKRAVILLYRENNKVRKKMVTSRSAHGDQDSFKFRWLIPLSALQVRLGNTADTDCLWELIHTKSQKEGRPETVFQLCSSTPECKVNIIKVMRSILRENLRRNMQRVEQSERTCKKRLTPLRKSLPASARIGSSRAFWLAKQPSNDSPVPSNDPLRLSLADSDSASASSGTTSSASAPLDPPSQQQSGLRESSIISDEDEDGSEPADWTDAPNAIEVQFRKLGLTEETSQAAAPRVQPESGEVDTPERKASLKRAHFAVVKRKPARDTNSLRRSQVALLNMRRHSHSLDIPAEIAAHSVDLNSLLEKDFSVQSLTSVVNEDFFYDNPMEPKNVTTS

Target ORF information:

RefSeq Version	XM_009293290.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio T-cell lymphoma invasion and metastasis 2a (tiam2a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293290.3

ORF Insert Sequence:

ATGGCAGAGC AGACCGAACG GGATGCCAGG CCATGCATCT ACCTCACGGC GCTCATCGAG 
CTCTGCCTCT GCGAGCACTC GCCCGTCGAG CTTCTCTGTT CCTTCTTTAC CATGTGCTGG 
CTCTGGCCAG ACTGTGTGAA GGAAGGCAAG GATATCTTCC TCAACTCGGC TGTGCTGACT 
CTCCACCGGC TACTCCAGAA CAAGCTGGCC CGACTGCTAG ATGAGATCGC CGCCTGCGTG 
GCCTGCCAGA ATGGTGCCGA AGGATCCATT TTCATGAGTG TGGACACGGT GTGTTCGCTG 
TACCACTCCT ACCAGGAAGG CTCTGGAGGT CTGATGGAGA GCCAGGAGGA GCAAACGGGG 
CCTGAACCCG CTATCCTCAG GCCCTGTCCG CGTCATATGT CTGCCACAGA GAGACTACGC 
AAGGTCATCC AGGAATTAGT GGATACAGAG AAATCCTACG TAAAGGACCT CAACTGCTTG 
TTTGAAATCT ACCTGAAGCC CCTGCAAAAG GAGACCTTTC TCACTCAAGA TGAGATGGAA 
AGCCTATTTG GCAGCCTTCC AGAGATGCTG GACTTCCAGA AAGTTTTCCT GCAGACCCTG 
GAAGAGAAGA TTAGTTCCTC CCCCAACTTC AACACGTTCG AGACCCCTGT CCAATTCAAG 
AAGCTGCTCT TCTCTCTGGG TGGCTCATTT CTGTATTATG CGGAGCACTT CAAACTGTAC 
AGCGGCTTCT GCGCCAACCA TATTAAAGTG CAGAAGGTTC TGGAACGAGC TAAAACAGAT 
CGATCTTTTA AAGAGTTCCT CGATGCTAGG AACCCGACCA AGCAGCACTC AACCACGCTA 
GAGTCCTACC TGATCAAACC AGTGCAGAGA GTGCTGAAAT ACCCCCTGCT GCTCCGCGAG 
CTGGTGTCTC TGACGGACAC GGACAGTGAG GAGCACTACC ACCTCACAGA GGCGCTTAAG 
GAAATGGAAA AGGTCGCAAG CCATATAAAC GAAATGCAGA AGATCTACGA AGACTACGGT 
GCAGTGTTTG ACCAGCTGGT GGCAGAGCAA AGTGGCACAG AGAAAGAGGT CACAGAGATA 
TCTATGGGAC AGTTCCTCAT GCATTCCTCT GTGATCTGGC TCAACCCGTT CCCATCGTTG 
GGCCGTATGA AAAAGGATCC CGAGCTGACT GTTTTTGTTT TTAAGAGAGC CGTGATACTG 
CTGTACCGGG AGAACAACAA GGTGAGGAAG AAGATGGTGA CTTCAAGATC AGCCCACGGA 
GATCAGGATT CATTCAAGTT TCGCTGGCTC ATTCCCCTGT CAGCGCTACA GGTCAGACTG 
GGCAACACTG CAGACACAGA CTGCCTCTGG GAGCTGATTC ACACCAAATC ACAAAAGGAG 
GGCAGGCCTG AGACGGTGTT CCAGTTGTGC AGCAGCACAC CAGAGTGCAA AGTGAATATC 
ATCAAGGTGA TGCGCTCCAT CTTGCGGGAG AACCTGCGTC GAAACATGCA AAGGGTTGAA 
CAGTCTGAGA GAACCTGTAA AAAGCGGCTT ACTCCCCTTC GGAAAAGCCT GCCAGCTTCG 
GCTAGGATAG GTTCTTCTCG AGCCTTTTGG CTAGCCAAAC AACCTTCAAA CGACAGTCCC 
GTTCCCAGCA ATGATCCACT GAGGCTGTCT CTAGCAGATT CTGATTCAGC CAGTGCTAGC 
AGTGGAACCA CCAGCAGTGC CAGCGCTCCT CTAGATCCGC CATCACAGCA GCAGAGCGGC 
CTCAGAGAAT CCAGCATAAT CAGCGATGAG GACGAAGACG GCAGCGAGCC GGCTGATTGG 
ACGGATGCTC CCAATGCTAT CGAGGTCCAA TTTCGCAAGC TTGGTCTGAC CGAGGAGACC 
TCCCAGGCAG CTGCTCCACG TGTGCAGCCG GAGAGCGGAG AAGTGGACAC TCCAGAAAGG 
AAGGCTTCTC TAAAGCGAGC TCACTTTGCT GTGGTCAAAC GCAAGCCCGC CAGAGACACG 
AATAGCCTCA GACGCAGTCA GGTGGCCCTG CTGAACATGC GTAGACACAG CCACTCTTTG 
GACATCCCAG CGGAGATCGC GGCACATAGT GTAGACCTGA ACTCGCTACT GGAGAAAGAT 
TTCAGCGTGC AGAGCCTGAC CTCTGTTGTC AACGAAGACT TCTTCTATGA CAATCCCATG 
GAGCCAAAGA ATGTGACAAC TTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tiam2a ( XM_021467481.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021323156.1 Danio rerio T-cell lymphoma invasion and metastasis 2a (tiam2a), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa149763
Clone ID Related Accession (Same CDS sequence)	XM_021467481.1
Accession Version	XM_021467481.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2022bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	T-lymphoma invasion and metastasis-inducing protein 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAACTT TCAGGGTGAG CAAGATTAGA AGGAAGGGCT GCTCCCTGGC CAGAAGGACC 
AAGTACCAGT CTATAGCTGC TTGGAGAATA CTATACAGGC TGCTTTTCAT GAGTGTGGAC 
ACGGTGTGTT CGCTGTACCA CTCCTACCAG GAAGGCTCTG GAGGTCTGAT GGAGAGCCAG 
GAGGAGCAAA CGGGGCCTGA ACCCGCTATC CTCAGGCCCT GTCCGCGTCA TATGTCTGCC 
ACAGAGAGAC TACGCAAGGT CATCCAGGAA TTAGTGGATA CAGAGAAATC CTACGTAAAG 
GACCTCAACT GCTTGTTTGA AATCTACCTG AAGCCCCTGC AAAAGGAGAC CTTTCTCACT 
CAAGATGAGA TGGAAAGCCT ATTTGGCAGC CTTCCAGAGA TGCTGGACTT CCAGAAAGTT 
TTCCTGCAGA CCCTGGAAGA GAAGATTAGT TCCTCCCCCA ACTTCAACAC GTTCGAGACC 
CCTGTCCAAT TCAAGAAGCT GCTCTTCTCT CTGGGTGGCT CATTTCTGTA TTATGCGGAG 
CACTTCAAAC TGTACAGCGG CTTCTGCGCC AACCATATTA AAGTGCAGAA GGTTCTGGAA 
CGAGCTAAAA CAGATCGATC TTTTAAAGAG TTCCTCGATG CTAGGAACCC GACCAAGCAG 
CACTCAACCA CGCTAGAGTC CTACCTGATC AAACCAGTGC AGAGAGTGCT GAAATACCCC 
CTGCTGCTCC GCGAGCTGGT GTCTCTGACG GACACGGACA GTGAGGAGCA CTACCACCTC 
ACAGAGGCGC TTAAGGAAAT GGAAAAGGTC GCAAGCCATA TAAACGAAAT GCAGAAGATC 
TACGAAGACT ACGGTGCAGT GTTTGACCAG CTGGTGGCAG AGCAAAGTGG CACAGAGAAA 
GAGGTCACAG AGATATCTAT GGGACAGTTC CTCATGCATT CCTCTGTGAT CTGGCTCAAC 
CCGTTCCCAT CGTTGGGCCG TATGAAAAAG GATCCCGAGC TGACTGTTTT TGTTTTTAAG 
AGAGCCGTGA TACTGCTGTA CCGGGAGAAC AACAAGGTGA GGAAGAAGAT GGTGACTTCA 
AGATCAGCCC ACGGAGATCA GGATTCATTC AAGTTTCGCT GGCTCATTCC CCTGTCAGCG 
CTACAGGTCA GACTGGGCAA CACTGCAGAC ACAGACTGCC TCTGGGAGCT GATTCACACC 
AAATCACAAA AGGAGGGCAG GCCTGAGACG GTGTTCCAGT TGTGCAGCAG CACACCAGAG 
TGCAAAGTGA ATATCATCAA GGTGATGCGC TCCATCTTGC GGGAGAACCT GCGTCGAAAC 
ATGCAAAGGG TTGAACAGTC TGAGAGAACC TGTAAAAAGC GGCTTACTCC CCTTCGGAAA 
AGCCTGCCAG CTTCGGCTAG GATAGGTTCT TCTCGAGCCT TTTGGCTAGC CAAACAACCT 
TCAAACGACA GTCCCGTTCC CAGCAATGAT CCACTGAGGC TGTCTCTAGC AGATTCTGAT 
TCAGCCAGTG CTAGCAGTGG AACCACCAGC AGTGCCAGCG CTCCTCTAGA TCCGCCATCA 
CAGCAGCAGA GCGGCCTCAG AGAATCCAGC ATAATCAGCG ATGAGGACGA AGACGGCAGC 
GAGCCGGCTG ATTGGACGGA TGCTCCCAAT GCTATCGAGG TCCAATTTCG CAAGCTTGGT 
CTGACCGAGG AGACCTCCCA GGCAGCTGCT CCACGTGTGC AGCCGGAGAG CGGAGAAGTG 
GACACTCCAG AAAGGAAGGC TTCTCTAAAG CGAGCTCACT TTGCTGTGGT CAAACGCAAG 
CCCGCCAGAG ACACGAATAG CCTCAGACGC AGTCAGGTGG CCCTGCTGAA CATGCGTAGA 
CACAGCCACT CTTTGGACAT CCCAGCGGAG ATCGCGGCAC ATAGTGTAGA CCTGAACTCG 
CTACTGGAGA AAGATTTCAG CGTGCAGAGC CTGACCTCTG TTGTCAACGA AGACTTCTTC 
TATGACAATC CCATGGAGCC AAAGAATGTG ACAACTTCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021323156.1
CDS	119..2140
Translation	MPTFRVSKIRRKGCSLARRTKYQSIAAWRILYRLLFMSVDTVCSLYHSYQEGSGGLMESQEEQTGPEPAILRPCPRHMSATERLRKVIQELVDTEKSYVKDLNCLFEIYLKPLQKETFLTQDEMESLFGSLPEMLDFQKVFLQTLEEKISSSPNFNTFETPVQFKKLLFSLGGSFLYYAEHFKLYSGFCANHIKVQKVLERAKTDRSFKEFLDARNPTKQHSTTLESYLIKPVQRVLKYPLLLRELVSLTDTDSEEHYHLTEALKEMEKVASHINEMQKIYEDYGAVFDQLVAEQSGTEKEVTEISMGQFLMHSSVIWLNPFPSLGRMKKDPELTVFVFKRAVILLYRENNKVRKKMVTSRSAHGDQDSFKFRWLIPLSALQVRLGNTADTDCLWELIHTKSQKEGRPETVFQLCSSTPECKVNIIKVMRSILRENLRRNMQRVEQSERTCKKRLTPLRKSLPASARIGSSRAFWLAKQPSNDSPVPSNDPLRLSLADSDSASASSGTTSSASAPLDPPSQQQSGLRESSIISDEDEDGSEPADWTDAPNAIEVQFRKLGLTEETSQAAAPRVQPESGEVDTPERKASLKRAHFAVVKRKPARDTNSLRRSQVALLNMRRHSHSLDIPAEIAAHSVDLNSLLEKDFSVQSLTSVVNEDFFYDNPMEPKNVTTS

Target ORF information:

RefSeq Version	XM_021467481.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio T-cell lymphoma invasion and metastasis 2a (tiam2a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021467481.1

ORF Insert Sequence:

ATGCCAACTT TCAGGGTGAG CAAGATTAGA AGGAAGGGCT GCTCCCTGGC CAGAAGGACC 
AAGTACCAGT CTATAGCTGC TTGGAGAATA CTATACAGGC TGCTTTTCAT GAGTGTGGAC 
ACGGTGTGTT CGCTGTACCA CTCCTACCAG GAAGGCTCTG GAGGTCTGAT GGAGAGCCAG 
GAGGAGCAAA CGGGGCCTGA ACCCGCTATC CTCAGGCCCT GTCCGCGTCA TATGTCTGCC 
ACAGAGAGAC TACGCAAGGT CATCCAGGAA TTAGTGGATA CAGAGAAATC CTACGTAAAG 
GACCTCAACT GCTTGTTTGA AATCTACCTG AAGCCCCTGC AAAAGGAGAC CTTTCTCACT 
CAAGATGAGA TGGAAAGCCT ATTTGGCAGC CTTCCAGAGA TGCTGGACTT CCAGAAAGTT 
TTCCTGCAGA CCCTGGAAGA GAAGATTAGT TCCTCCCCCA ACTTCAACAC GTTCGAGACC 
CCTGTCCAAT TCAAGAAGCT GCTCTTCTCT CTGGGTGGCT CATTTCTGTA TTATGCGGAG 
CACTTCAAAC TGTACAGCGG CTTCTGCGCC AACCATATTA AAGTGCAGAA GGTTCTGGAA 
CGAGCTAAAA CAGATCGATC TTTTAAAGAG TTCCTCGATG CTAGGAACCC GACCAAGCAG 
CACTCAACCA CGCTAGAGTC CTACCTGATC AAACCAGTGC AGAGAGTGCT GAAATACCCC 
CTGCTGCTCC GCGAGCTGGT GTCTCTGACG GACACGGACA GTGAGGAGCA CTACCACCTC 
ACAGAGGCGC TTAAGGAAAT GGAAAAGGTC GCAAGCCATA TAAACGAAAT GCAGAAGATC 
TACGAAGACT ACGGTGCAGT GTTTGACCAG CTGGTGGCAG AGCAAAGTGG CACAGAGAAA 
GAGGTCACAG AGATATCTAT GGGACAGTTC CTCATGCATT CCTCTGTGAT CTGGCTCAAC 
CCGTTCCCAT CGTTGGGCCG TATGAAAAAG GATCCCGAGC TGACTGTTTT TGTTTTTAAG 
AGAGCCGTGA TACTGCTGTA CCGGGAGAAC AACAAGGTGA GGAAGAAGAT GGTGACTTCA 
AGATCAGCCC ACGGAGATCA GGATTCATTC AAGTTTCGCT GGCTCATTCC CCTGTCAGCG 
CTACAGGTCA GACTGGGCAA CACTGCAGAC ACAGACTGCC TCTGGGAGCT GATTCACACC 
AAATCACAAA AGGAGGGCAG GCCTGAGACG GTGTTCCAGT TGTGCAGCAG CACACCAGAG 
TGCAAAGTGA ATATCATCAA GGTGATGCGC TCCATCTTGC GGGAGAACCT GCGTCGAAAC 
ATGCAAAGGG TTGAACAGTC TGAGAGAACC TGTAAAAAGC GGCTTACTCC CCTTCGGAAA 
AGCCTGCCAG CTTCGGCTAG GATAGGTTCT TCTCGAGCCT TTTGGCTAGC CAAACAACCT 
TCAAACGACA GTCCCGTTCC CAGCAATGAT CCACTGAGGC TGTCTCTAGC AGATTCTGAT 
TCAGCCAGTG CTAGCAGTGG AACCACCAGC AGTGCCAGCG CTCCTCTAGA TCCGCCATCA 
CAGCAGCAGA GCGGCCTCAG AGAATCCAGC ATAATCAGCG ATGAGGACGA AGACGGCAGC 
GAGCCGGCTG ATTGGACGGA TGCTCCCAAT GCTATCGAGG TCCAATTTCG CAAGCTTGGT 
CTGACCGAGG AGACCTCCCA GGCAGCTGCT CCACGTGTGC AGCCGGAGAG CGGAGAAGTG 
GACACTCCAG AAAGGAAGGC TTCTCTAAAG CGAGCTCACT TTGCTGTGGT CAAACGCAAG 
CCCGCCAGAG ACACGAATAG CCTCAGACGC AGTCAGGTGG CCCTGCTGAA CATGCGTAGA 
CACAGCCACT CTTTGGACAT CCCAGCGGAG ATCGCGGCAC ATAGTGTAGA CCTGAACTCG 
CTACTGGAGA AAGATTTCAG CGTGCAGAGC CTGACCTCTG TTGTCAACGA AGACTTCTTC 
TATGACAATC CCATGGAGCC AAAGAATGTG ACAACTTCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tiam2a ( XM_021467480.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021323155.1 Danio rerio T-cell lymphoma invasion and metastasis 2a (tiam2a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa49964
Clone ID Related Accession (Same CDS sequence)	XM_021467480.1 , XM_009293288.2 , XM_009293288.3
Accession Version	XM_021467480.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	T-lymphoma invasion and metastasis-inducing protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAACT CGGAGAGTCA GTATAGGATC CAGGGCACTA GAAGCAATAG CTTTATTATC 
CCTGACCGAG AGAAACCCTG TTCATTCAGC GTCCACTCCA GCAAAGAAGA CGTTTTATCA 
CCTTTAGGCT TTTGGAATGC TGGTTCAAGA TTTTCTAGAT TGAAATCCGG AAATGAAGGT 
GAAACTTCCT CCACGTCTAA GAGCAGCCGG GCGTTTATAT CCCGCCAGTA TGACTTCATC 
ACCCAGAAAC TAAAGAGTGG TAGTCAGTGG GATGAAGTTC CTGAGGACCT GTTGCCTGGT 
GGCTGCTTGA TGTTGCCTTC GGAAAAGGAC TGCTTGTATC GCAATCACAA GGAGAGGAGA 
AAAACATGTA ATAAATATGC TCTTAATGCA ATGAAGACAC CTGAGAGAAG TCTCAGACTT 
GAGGACATTG AAGACCAGAG CAGCCCTAGG GTTGTCATAA AGAAAGATGG TAGCGTACGT 
GTGGAATTTA ACAATAGTCC CAATAGTGCC CTTATTTTAA ATAATTGCAC TGAACCAGTT 
CAGCTCCTCA AATTTTCCCC CACCATGGAG TCCACTTGTG GTTTATCTGC TGCAGGACCC 
TCATTGGATT CTCATAATGG AGCAGCACAG GCAATGTCCA GCTTGAAAAG CAGCAAAGGT 
AGCTCTCTGA GCTCAGATGG CTCTTTGTAC GACTTGCCTT GGAGGACCAG CGTCGAACTT 
AATGATTCAG ACAATGCTTC TCCTGACCAG CAAAGGCAAA CTGTTTCTAC AAACACAGGC 
TTTTTTGATG AGCAGTTGCC AGCCATTACT ACAACCGACC TCTACAGGGA TCCCAGAATT 
GCTTCCACCT TCCCCACGGT CAAGGATTTG CAGTGTTCAA ATGGCAGCCG TTCACACCAC 
AGATCCTCCT TTGTGTCCGT GATGGAGGGA CTCAGTTCAG AAGGGGTCCC AGAGGAACAG 
CAGTATTCCT CCTTCACCTT GCCTTGCCGG AAATCCAAAG CTTCTCCTGA AAATAATGGA 
AAGAAAGCGT CGATTAGGAG CAGATTTAGA CGCATCAGTG ACTGGACCGG AAGTCTGACC 
AGGAAAAAGA AGTTTCAGGA GCCCAGATAT AACGAGCCGG TCGATTGCTT TGACAGCGGT 
GTGGATGGAC TCACTGCAGA TACCAGTTCC CCCTCTCAGG TCTCCAGCCT TATGGCTACC 
TCCAATGGCA TGTCTCAATC CTGCTCTCTC TCTCGCTCGC TGCACAGCCG CAGTGGCTCT 
GCCGGAGCCC TTAATTTAGG AAATGACATT CTACGGCAAA ACATCTACAA GAATTTTATG 
ATGGAGTTAA AGACTGCCCG GCTTCCAGCA GAAAGAGGAG CTGAAGATTG GTGTGAGAGC 
TCCTCAGAGG AAACAGGAAG CAGTAGTCAA GAAATCCCGG GGTCTCTGGA GCAGCTGGAT 
TTGCTGTTCG AGAAAGAACA AGGGGTAGTT AGGCGAGCTG GGTGGCTGTC CTTCAAGCCC 
TTGCTCACTG TACACAAGGA CCGCAAACTG GAGCTAGTGA GCCGTCGAAA ATGGAAGCAA 
TTCTGGGTCA CTCTCAAGGG TTGCACACTG CTGTTTTATG AAACCTATGG AAAGGCATCT 
TCAGAGCCAG AATCTCCATG CTATGCCTTG TTCGCAGAAG ATGGCGTAGT CCAGGCTGTT 
CCCGAACACC CTAAGAAGGA GAATATCTTC TGCCTTAGTA ACACCTATGG CGATGTCTAC 
CTTTTCCAGG CCTCCAGTCA GACTGACCTA GAGAACTGGG TTACTGCCAT TCATTCAGCA 
AGTGCCTCTT TGTTTGCGAA ACGCTACGGC AAAGAGGACA CACTGCGATT GTTACGTGGG 
CAGATCCGTG GCCTTCTCCA GAAAATCGAC ATGGATGGCA AAATGAAGAA AATGGCCGAG 
CTTCAGCTGT CCGTCGTTGG GGACCCCAAA AACAGAAAAG CTATTGAAAA CCAGATTCAA 
CAATGGGAGC AGAATCTCGA GCGCTTCAAT ATGGACCTTT TCCGCATGCG ATGCTACCTG 
GCCAGTCTGC AAGGAGGCGA GCTCCCCAAC CCAAAGAGCC TCCTAGCCAC GGCCAGCCGC 
CGCACCAAAG CTGCACTAGC CCGATTGGGA ATCTTCTCCG TCTCCTCCTT CCATGCACTG 
ATCTGTTCCA GAGATGAGGC CACGATGCGA AAGCGCTCTC TGTCTCTCTC CCACAGAGGC 
CGCAGCAAGA AAGGGGTCTT TTCATCCCTT AAGGGCCTGG ATACTCTTAC CAAGCGAGGC 
CATGAAAAAA AGCATTCTCT CTCACAGGAC CTCAGTGAAT TGAGCTGCTG TTCTTACAGT 
GGTCTTCCGC AGGTCTTTGA CAGTGATGGC GGAGCGCAGC TAAATTCCGT TACACATTGT 
AGTTTTAAGC GGTTAGGCAG TTTGACGAAT GTTTACTCAA TTGTGCCACC TGAAGGCAGC 
CAGTGGGATT GCAGTCGAGA AAGCACAACA TGCATCTACA TGCCAAACTG CCAGACTGTT 
ACTGTTCTCC TGAAAAGTCA TCACACTGTC ATGGATGTGT TGGCTGAGAC CTGCATGCTC 
GTGGGCCTGG ACCCCAGCAC ACACTGTTTG AGGCTGAGAA GATTCACAGG AGACAAGATG 
GAGATCTCAA CACCATCAGC TCATGAGCTT CTGCAAGGCT TGCCATATGA CGAATTGGAA 
GTTAGCCAGC TGCAAGTCTA CACTCTGCAC ATGTCTAGAC CCAGTCTAAC TGGAGACTTT 
GGCTTTGCTG TTACAGGCTA TGTAGACAGC CTCAAAAACA GCCATATCAT TGTCAGCGAA 
GTTCTCCCTG ATGGGCTCGC TTTCAGCAAA GGTCTTAGGC CTGGTGATGA GATCGTGGTT 
CTGAACGGTT GTGAGGTGTC TTCTCTGGAC CTGACTCTCA TACAGACTTT ATTTAATGAG 
CAGAATCTGC AGCTGACTGC GAAACGTCAC CTGCCCTCCA TTCCACATGA TTCAGACACA 
GCAAACGTGC AGTCCCCGTA CAGAGATATC CATCAAAACC GCCAGAGAGC CAAGTCCTCA 
ACAGATGTGT CCTGTGTGAC AGAAACCTTG TCTGTAAATG ATGGTCTTCA AGATTCTTAC 
CACAACCATC GCCAAGTTCA TCACAGCAAG AGTGTGGACA CGGTGTGTTC GCTGTACCAC 
TCCTACCAGG AAGGCTCTGG AGGTCTGATG GAGAGCCAGG AGGAGCAAAC GGGGCCTGAA 
CCCGCTATCC TCAGGCCCTG TCCGCGTCAT ATGTCTGCCA CAGAGAGACT ACGCAAGGTC 
ATCCAGGAAT TAGTGGATAC AGAGAAATCC TACGTAAAGG ACCTCAACTG CTTGTTTGAA 
ATCTACCTGA AGCCCCTGCA AAAGGAGACC TTTCTCACTC AAGATGAGAT GGAAAGCCTA 
TTTGGCAGCC TTCCAGAGAT GCTGGACTTC CAGAAAGTTT TCCTGCAGAC CCTGGAAGAG 
AAGATTAGTT CCTCCCCCAA CTTCAACACG TTCGAGACCC CTGTCCAATT CAAGAAGCTG 
CTCTTCTCTC TGGGTGGCTC ATTTCTGTAT TATGCGGAGC ACTTCAAACT GTACAGCGGC 
TTCTGCGCCA ACCATATTAA AGTGCAGAAG GTTCTGGAAC GAGCTAAAAC AGATCGATCT 
TTTAAAGAGT TCCTCGATGC TAGGAACCCG ACCAAGCAGC ACTCAACCAC GCTAGAGTCC 
TACCTGATCA AACCAGTGCA GAGAGTGCTG AAATACCCCC TGCTGCTCCG CGAGCTGGTG 
TCTCTGACGG ACACGGACAG TGAGGAGCAC TACCACCTCA CAGAGGCGCT TAAGGAAATG 
GAAAAGGTCG CAAGCCATAT AAACGAAATG CAGAAGATCT ACGAAGACTA CGGTGCAGTG 
TTTGACCAGC TGGTGGCAGA GCAAAGTGGC ACAGAGAAAG AGGTCACAGA GATATCTATG 
GGACAGTTCC TCATGCATTC CTCTGTGATC TGGCTCAACC CGTTCCCATC GTTGGGCCGT 
ATGAAAAAGG ATCCCGAGCT GACTGTTTTT GTTTTTAAGA GAGCCGTGAT ACTGCTGTAC 
CGGGAGAACA ACAAGGTGAG GAAGAAGATG GTGACTTCAA GATCAGCCCA CGGAGATCAG 
GATTCATTCA AGTTTCGCTG GCTCATTCCC CTGTCAGCGC TACAGGTCAG ACTGGGCAAC 
ACTGCAGACA CAGACTGCCT CTGGGAGCTG ATTCACACCA AATCACAAAA GGAGGGCAGG 
CCTGAGACGG TGTTCCAGTT GTGCAGCAGC ACACCAGAGT GCAAAGTGAA TATCATCAAG 
GTGATGCGCT CCATCTTGCG GGAGAACCTG CGTCGAAACA TGCAAAGGGT TGAACAGTCT 
GAGAGAACCT GTAAAAAGCG GCTTACTCCC CTTCGGAAAA GCCTGCCAGC TTCGGCTAGG 
ATAGGTTCTT CTCGAGCCTT TTGGCTAGCC AAACAACCTT CAAACGACAG TCCCGTTCCC 
AGCAATGATC CACTGAGGCT GTCTCTAGCA GATTCTGATT CAGCCAGTGC TAGCAGTGGA 
ACCACCAGCA GTGCCAGCGC TCCTCTAGAT CCGCCATCAC AGCAGCAGAG CGGCCTCAGA 
GAATCCAGCA TAATCAGCGA TGAGGACGAA GACGGCAGCG AGCCGGCTGA TTGGACGGAT 
GCTCCCAATG CTATCGAGGT CCAATTTCGC AAGCTTGGTC TGACCGAGGA GACCTCCCAG 
GCAGCTGCTC CACGTGTGCA GCCGGAGAGC GGAGAAGTGG ACACTCCAGA AAGGAAGGCT 
TCTCTAAAGC GAGCTCACTT TGCTGTGGTC AAACGCAAGC CCGCCAGAGA CACGAATAGC 
CTCAGACGCA GTCAGGTGGC CCTGCTGAAC ATGCGTAGAC ACAGCCACTC TTTGGACATC 
CCAGCGGAGA TCGCGGCACA TAGTGTAGAC CTGAACTCGC TACTGGAGAA AGATTTCAGC 
GTGCAGAGCC TGACCTCTGT TGTCAACGAA GACTTCTTCT ATGACAATCC CATGGAGCCA 
AAGAATGTGA CAACTTCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021323155.1
CDS	310..5370
Translation	MGNSESQYRIQGTRSNSFIIPDREKPCSFSVHSSKEDVLSPLGFWNAGSRFSRLKSGNEGETSSTSKSSRAFISRQYDFITQKLKSGSQWDEVPEDLLPGGCLMLPSEKDCLYRNHKERRKTCNKYALNAMKTPERSLRLEDIEDQSSPRVVIKKDGSVRVEFNNSPNSALILNNCTEPVQLLKFSPTMESTCGLSAAGPSLDSHNGAAQAMSSLKSSKGSSLSSDGSLYDLPWRTSVELNDSDNASPDQQRQTVSTNTGFFDEQLPAITTTDLYRDPRIASTFPTVKDLQCSNGSRSHHRSSFVSVMEGLSSEGVPEEQQYSSFTLPCRKSKASPENNGKKASIRSRFRRISDWTGSLTRKKKFQEPRYNEPVDCFDSGVDGLTADTSSPSQVSSLMATSNGMSQSCSLSRSLHSRSGSAGALNLGNDILRQNIYKNFMMELKTARLPAERGAEDWCESSSEETGSSSQEIPGSLEQLDLLFEKEQGVVRRAGWLSFKPLLTVHKDRKLELVSRRKWKQFWVTLKGCTLLFYETYGKASSEPESPCYALFAEDGVVQAVPEHPKKENIFCLSNTYGDVYLFQASSQTDLENWVTAIHSASASLFAKRYGKEDTLRLLRGQIRGLLQKIDMDGKMKKMAELQLSVVGDPKNRKAIENQIQQWEQNLERFNMDLFRMRCYLASLQGGELPNPKSLLATASRRTKAALARLGIFSVSSFHALICSRDEATMRKRSLSLSHRGRSKKGVFSSLKGLDTLTKRGHEKKHSLSQDLSELSCCSYSGLPQVFDSDGGAQLNSVTHCSFKRLGSLTNVYSIVPPEGSQWDCSRESTTCIYMPNCQTVTVLLKSHHTVMDVLAETCMLVGLDPSTHCLRLRRFTGDKMEISTPSAHELLQGLPYDELEVSQLQVYTLHMSRPSLTGDFGFAVTGYVDSLKNSHIIVSEVLPDGLAFSKGLRPGDEIVVLNGCEVSSLDLTLIQTLFNEQNLQLTAKRHLPSIPHDSDTANVQSPYRDIHQNRQRAKSSTDVSCVTETLSVNDGLQDSYHNHRQVHHSKSVDTVCSLYHSYQEGSGGLMESQEEQTGPEPAILRPCPRHMSATERLRKVIQELVDTEKSYVKDLNCLFEIYLKPLQKETFLTQDEMESLFGSLPEMLDFQKVFLQTLEEKISSSPNFNTFETPVQFKKLLFSLGGSFLYYAEHFKLYSGFCANHIKVQKVLERAKTDRSFKEFLDARNPTKQHSTTLESYLIKPVQRVLKYPLLLRELVSLTDTDSEEHYHLTEALKEMEKVASHINEMQKIYEDYGAVFDQLVAEQSGTEKEVTEISMGQFLMHSSVIWLNPFPSLGRMKKDPELTVFVFKRAVILLYRENNKVRKKMVTSRSAHGDQDSFKFRWLIPLSALQVRLGNTADTDCLWELIHTKSQKEGRPETVFQLCSSTPECKVNIIKVMRSILRENLRRNMQRVEQSERTCKKRLTPLRKSLPASARIGSSRAFWLAKQPSNDSPVPSNDPLRLSLADSDSASASSGTTSSASAPLDPPSQQQSGLRESSIISDEDEDGSEPADWTDAPNAIEVQFRKLGLTEETSQAAAPRVQPESGEVDTPERKASLKRAHFAVVKRKPARDTNSLRRSQVALLNMRRHSHSLDIPAEIAAHSVDLNSLLEKDFSVQSLTSVVNEDFFYDNPMEPKNVTTS

Target ORF information:

RefSeq Version	XM_021467480.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio T-cell lymphoma invasion and metastasis 2a (tiam2a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021467480.1

ORF Insert Sequence:

ATGGGAAACT CGGAGAGTCA GTATAGGATC CAGGGCACTA GAAGCAATAG CTTTATTATC 
CCTGACCGAG AGAAACCCTG TTCATTCAGC GTCCACTCCA GCAAAGAAGA CGTTTTATCA 
CCTTTAGGCT TTTGGAATGC TGGTTCAAGA TTTTCTAGAT TGAAATCCGG AAATGAAGGT 
GAAACTTCCT CCACGTCTAA GAGCAGCCGG GCGTTTATAT CCCGCCAGTA TGACTTCATC 
ACCCAGAAAC TAAAGAGTGG TAGTCAGTGG GATGAAGTTC CTGAGGACCT GTTGCCTGGT 
GGCTGCTTGA TGTTGCCTTC GGAAAAGGAC TGCTTGTATC GCAATCACAA GGAGAGGAGA 
AAAACATGTA ATAAATATGC TCTTAATGCA ATGAAGACAC CTGAGAGAAG TCTCAGACTT 
GAGGACATTG AAGACCAGAG CAGCCCTAGG GTTGTCATAA AGAAAGATGG TAGCGTACGT 
GTGGAATTTA ACAATAGTCC CAATAGTGCC CTTATTTTAA ATAATTGCAC TGAACCAGTT 
CAGCTCCTCA AATTTTCCCC CACCATGGAG TCCACTTGTG GTTTATCTGC TGCAGGACCC 
TCATTGGATT CTCATAATGG AGCAGCACAG GCAATGTCCA GCTTGAAAAG CAGCAAAGGT 
AGCTCTCTGA GCTCAGATGG CTCTTTGTAC GACTTGCCTT GGAGGACCAG CGTCGAACTT 
AATGATTCAG ACAATGCTTC TCCTGACCAG CAAAGGCAAA CTGTTTCTAC AAACACAGGC 
TTTTTTGATG AGCAGTTGCC AGCCATTACT ACAACCGACC TCTACAGGGA TCCCAGAATT 
GCTTCCACCT TCCCCACGGT CAAGGATTTG CAGTGTTCAA ATGGCAGCCG TTCACACCAC 
AGATCCTCCT TTGTGTCCGT GATGGAGGGA CTCAGTTCAG AAGGGGTCCC AGAGGAACAG 
CAGTATTCCT CCTTCACCTT GCCTTGCCGG AAATCCAAAG CTTCTCCTGA AAATAATGGA 
AAGAAAGCGT CGATTAGGAG CAGATTTAGA CGCATCAGTG ACTGGACCGG AAGTCTGACC 
AGGAAAAAGA AGTTTCAGGA GCCCAGATAT AACGAGCCGG TCGATTGCTT TGACAGCGGT 
GTGGATGGAC TCACTGCAGA TACCAGTTCC CCCTCTCAGG TCTCCAGCCT TATGGCTACC 
TCCAATGGCA TGTCTCAATC CTGCTCTCTC TCTCGCTCGC TGCACAGCCG CAGTGGCTCT 
GCCGGAGCCC TTAATTTAGG AAATGACATT CTACGGCAAA ACATCTACAA GAATTTTATG 
ATGGAGTTAA AGACTGCCCG GCTTCCAGCA GAAAGAGGAG CTGAAGATTG GTGTGAGAGC 
TCCTCAGAGG AAACAGGAAG CAGTAGTCAA GAAATCCCGG GGTCTCTGGA GCAGCTGGAT 
TTGCTGTTCG AGAAAGAACA AGGGGTAGTT AGGCGAGCTG GGTGGCTGTC CTTCAAGCCC 
TTGCTCACTG TACACAAGGA CCGCAAACTG GAGCTAGTGA GCCGTCGAAA ATGGAAGCAA 
TTCTGGGTCA CTCTCAAGGG TTGCACACTG CTGTTTTATG AAACCTATGG AAAGGCATCT 
TCAGAGCCAG AATCTCCATG CTATGCCTTG TTCGCAGAAG ATGGCGTAGT CCAGGCTGTT 
CCCGAACACC CTAAGAAGGA GAATATCTTC TGCCTTAGTA ACACCTATGG CGATGTCTAC 
CTTTTCCAGG CCTCCAGTCA GACTGACCTA GAGAACTGGG TTACTGCCAT TCATTCAGCA 
AGTGCCTCTT TGTTTGCGAA ACGCTACGGC AAAGAGGACA CACTGCGATT GTTACGTGGG 
CAGATCCGTG GCCTTCTCCA GAAAATCGAC ATGGATGGCA AAATGAAGAA AATGGCCGAG 
CTTCAGCTGT CCGTCGTTGG GGACCCCAAA AACAGAAAAG CTATTGAAAA CCAGATTCAA 
CAATGGGAGC AGAATCTCGA GCGCTTCAAT ATGGACCTTT TCCGCATGCG ATGCTACCTG 
GCCAGTCTGC AAGGAGGCGA GCTCCCCAAC CCAAAGAGCC TCCTAGCCAC GGCCAGCCGC 
CGCACCAAAG CTGCACTAGC CCGATTGGGA ATCTTCTCCG TCTCCTCCTT CCATGCACTG 
ATCTGTTCCA GAGATGAGGC CACGATGCGA AAGCGCTCTC TGTCTCTCTC CCACAGAGGC 
CGCAGCAAGA AAGGGGTCTT TTCATCCCTT AAGGGCCTGG ATACTCTTAC CAAGCGAGGC 
CATGAAAAAA AGCATTCTCT CTCACAGGAC CTCAGTGAAT TGAGCTGCTG TTCTTACAGT 
GGTCTTCCGC AGGTCTTTGA CAGTGATGGC GGAGCGCAGC TAAATTCCGT TACACATTGT 
AGTTTTAAGC GGTTAGGCAG TTTGACGAAT GTTTACTCAA TTGTGCCACC TGAAGGCAGC 
CAGTGGGATT GCAGTCGAGA AAGCACAACA TGCATCTACA TGCCAAACTG CCAGACTGTT 
ACTGTTCTCC TGAAAAGTCA TCACACTGTC ATGGATGTGT TGGCTGAGAC CTGCATGCTC 
GTGGGCCTGG ACCCCAGCAC ACACTGTTTG AGGCTGAGAA GATTCACAGG AGACAAGATG 
GAGATCTCAA CACCATCAGC TCATGAGCTT CTGCAAGGCT TGCCATATGA CGAATTGGAA 
GTTAGCCAGC TGCAAGTCTA CACTCTGCAC ATGTCTAGAC CCAGTCTAAC TGGAGACTTT 
GGCTTTGCTG TTACAGGCTA TGTAGACAGC CTCAAAAACA GCCATATCAT TGTCAGCGAA 
GTTCTCCCTG ATGGGCTCGC TTTCAGCAAA GGTCTTAGGC CTGGTGATGA GATCGTGGTT 
CTGAACGGTT GTGAGGTGTC TTCTCTGGAC CTGACTCTCA TACAGACTTT ATTTAATGAG 
CAGAATCTGC AGCTGACTGC GAAACGTCAC CTGCCCTCCA TTCCACATGA TTCAGACACA 
GCAAACGTGC AGTCCCCGTA CAGAGATATC CATCAAAACC GCCAGAGAGC CAAGTCCTCA 
ACAGATGTGT CCTGTGTGAC AGAAACCTTG TCTGTAAATG ATGGTCTTCA AGATTCTTAC 
CACAACCATC GCCAAGTTCA TCACAGCAAG AGTGTGGACA CGGTGTGTTC GCTGTACCAC 
TCCTACCAGG AAGGCTCTGG AGGTCTGATG GAGAGCCAGG AGGAGCAAAC GGGGCCTGAA 
CCCGCTATCC TCAGGCCCTG TCCGCGTCAT ATGTCTGCCA CAGAGAGACT ACGCAAGGTC 
ATCCAGGAAT TAGTGGATAC AGAGAAATCC TACGTAAAGG ACCTCAACTG CTTGTTTGAA 
ATCTACCTGA AGCCCCTGCA AAAGGAGACC TTTCTCACTC AAGATGAGAT GGAAAGCCTA 
TTTGGCAGCC TTCCAGAGAT GCTGGACTTC CAGAAAGTTT TCCTGCAGAC CCTGGAAGAG 
AAGATTAGTT CCTCCCCCAA CTTCAACACG TTCGAGACCC CTGTCCAATT CAAGAAGCTG 
CTCTTCTCTC TGGGTGGCTC ATTTCTGTAT TATGCGGAGC ACTTCAAACT GTACAGCGGC 
TTCTGCGCCA ACCATATTAA AGTGCAGAAG GTTCTGGAAC GAGCTAAAAC AGATCGATCT 
TTTAAAGAGT TCCTCGATGC TAGGAACCCG ACCAAGCAGC ACTCAACCAC GCTAGAGTCC 
TACCTGATCA AACCAGTGCA GAGAGTGCTG AAATACCCCC TGCTGCTCCG CGAGCTGGTG 
TCTCTGACGG ACACGGACAG TGAGGAGCAC TACCACCTCA CAGAGGCGCT TAAGGAAATG 
GAAAAGGTCG CAAGCCATAT AAACGAAATG CAGAAGATCT ACGAAGACTA CGGTGCAGTG 
TTTGACCAGC TGGTGGCAGA GCAAAGTGGC ACAGAGAAAG AGGTCACAGA GATATCTATG 
GGACAGTTCC TCATGCATTC CTCTGTGATC TGGCTCAACC CGTTCCCATC GTTGGGCCGT 
ATGAAAAAGG ATCCCGAGCT GACTGTTTTT GTTTTTAAGA GAGCCGTGAT ACTGCTGTAC 
CGGGAGAACA ACAAGGTGAG GAAGAAGATG GTGACTTCAA GATCAGCCCA CGGAGATCAG 
GATTCATTCA AGTTTCGCTG GCTCATTCCC CTGTCAGCGC TACAGGTCAG ACTGGGCAAC 
ACTGCAGACA CAGACTGCCT CTGGGAGCTG ATTCACACCA AATCACAAAA GGAGGGCAGG 
CCTGAGACGG TGTTCCAGTT GTGCAGCAGC ACACCAGAGT GCAAAGTGAA TATCATCAAG 
GTGATGCGCT CCATCTTGCG GGAGAACCTG CGTCGAAACA TGCAAAGGGT TGAACAGTCT 
GAGAGAACCT GTAAAAAGCG GCTTACTCCC CTTCGGAAAA GCCTGCCAGC TTCGGCTAGG 
ATAGGTTCTT CTCGAGCCTT TTGGCTAGCC AAACAACCTT CAAACGACAG TCCCGTTCCC 
AGCAATGATC CACTGAGGCT GTCTCTAGCA GATTCTGATT CAGCCAGTGC TAGCAGTGGA 
ACCACCAGCA GTGCCAGCGC TCCTCTAGAT CCGCCATCAC AGCAGCAGAG CGGCCTCAGA 
GAATCCAGCA TAATCAGCGA TGAGGACGAA GACGGCAGCG AGCCGGCTGA TTGGACGGAT 
GCTCCCAATG CTATCGAGGT CCAATTTCGC AAGCTTGGTC TGACCGAGGA GACCTCCCAG 
GCAGCTGCTC CACGTGTGCA GCCGGAGAGC GGAGAAGTGG ACACTCCAGA AAGGAAGGCT 
TCTCTAAAGC GAGCTCACTT TGCTGTGGTC AAACGCAAGC CCGCCAGAGA CACGAATAGC 
CTCAGACGCA GTCAGGTGGC CCTGCTGAAC ATGCGTAGAC ACAGCCACTC TTTGGACATC 
CCAGCGGAGA TCGCGGCACA TAGTGTAGAC CTGAACTCGC TACTGGAGAA AGATTTCAGC 
GTGCAGAGCC TGACCTCTGT TGTCAACGAA GACTTCTTCT ATGACAATCC CATGGAGCCA 
AAGAATGTGA CAACTTCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tiam2a ( XM_009293288.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009291563.1 Danio rerio T-cell lymphoma invasion and metastasis 2a (tiam2a), transcript variant X1, mRNA.

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CloneID	ODa49964
Clone ID Related Accession (Same CDS sequence)	XM_021467480.1 , XM_009293288.2 , XM_009293288.3
Accession Version	XM_009293288.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	T-lymphoma invasion and metastasis-inducing protein 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293288.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAACT CGGAGAGTCA GTATAGGATC CAGGGCACTA GAAGCAATAG CTTTATTATC 
CCTGACCGAG AGAAACCCTG TTCATTCAGC GTCCACTCCA GCAAAGAAGA CGTTTTATCA 
CCTTTAGGCT TTTGGAATGC TGGTTCAAGA TTTTCTAGAT TGAAATCCGG AAATGAAGGT 
GAAACTTCCT CCACGTCTAA GAGCAGCCGG GCGTTTATAT CCCGCCAGTA TGACTTCATC 
ACCCAGAAAC TAAAGAGTGG TAGTCAGTGG GATGAAGTTC CTGAGGACCT GTTGCCTGGT 
GGCTGCTTGA TGTTGCCTTC GGAAAAGGAC TGCTTGTATC GCAATCACAA GGAGAGGAGA 
AAAACATGTA ATAAATATGC TCTTAATGCA ATGAAGACAC CTGAGAGAAG TCTCAGACTT 
GAGGACATTG AAGACCAGAG CAGCCCTAGG GTTGTCATAA AGAAAGATGG TAGCGTACGT 
GTGGAATTTA ACAATAGTCC CAATAGTGCC CTTATTTTAA ATAATTGCAC TGAACCAGTT 
CAGCTCCTCA AATTTTCCCC CACCATGGAG TCCACTTGTG GTTTATCTGC TGCAGGACCC 
TCATTGGATT CTCATAATGG AGCAGCACAG GCAATGTCCA GCTTGAAAAG CAGCAAAGGT 
AGCTCTCTGA GCTCAGATGG CTCTTTGTAC GACTTGCCTT GGAGGACCAG CGTCGAACTT 
AATGATTCAG ACAATGCTTC TCCTGACCAG CAAAGGCAAA CTGTTTCTAC AAACACAGGC 
TTTTTTGATG AGCAGTTGCC AGCCATTACT ACAACCGACC TCTACAGGGA TCCCAGAATT 
GCTTCCACCT TCCCCACGGT CAAGGATTTG CAGTGTTCAA ATGGCAGCCG TTCACACCAC 
AGATCCTCCT TTGTGTCCGT GATGGAGGGA CTCAGTTCAG AAGGGGTCCC AGAGGAACAG 
CAGTATTCCT CCTTCACCTT GCCTTGCCGG AAATCCAAAG CTTCTCCTGA AAATAATGGA 
AAGAAAGCGT CGATTAGGAG CAGATTTAGA CGCATCAGTG ACTGGACCGG AAGTCTGACC 
AGGAAAAAGA AGTTTCAGGA GCCCAGATAT AACGAGCCGG TCGATTGCTT TGACAGCGGT 
GTGGATGGAC TCACTGCAGA TACCAGTTCC CCCTCTCAGG TCTCCAGCCT TATGGCTACC 
TCCAATGGCA TGTCTCAATC CTGCTCTCTC TCTCGCTCGC TGCACAGCCG CAGTGGCTCT 
GCCGGAGCCC TTAATTTAGG AAATGACATT CTACGGCAAA ACATCTACAA GAATTTTATG 
ATGGAGTTAA AGACTGCCCG GCTTCCAGCA GAAAGAGGAG CTGAAGATTG GTGTGAGAGC 
TCCTCAGAGG AAACAGGAAG CAGTAGTCAA GAAATCCCGG GGTCTCTGGA GCAGCTGGAT 
TTGCTGTTCG AGAAAGAACA AGGGGTAGTT AGGCGAGCTG GGTGGCTGTC CTTCAAGCCC 
TTGCTCACTG TACACAAGGA CCGCAAACTG GAGCTAGTGA GCCGTCGAAA ATGGAAGCAA 
TTCTGGGTCA CTCTCAAGGG TTGCACACTG CTGTTTTATG AAACCTATGG AAAGGCATCT 
TCAGAGCCAG AATCTCCATG CTATGCCTTG TTCGCAGAAG ATGGCGTAGT CCAGGCTGTT 
CCCGAACACC CTAAGAAGGA GAATATCTTC TGCCTTAGTA ACACCTATGG CGATGTCTAC 
CTTTTCCAGG CCTCCAGTCA GACTGACCTA GAGAACTGGG TTACTGCCAT TCATTCAGCA 
AGTGCCTCTT TGTTTGCGAA ACGCTACGGC AAAGAGGACA CACTGCGATT GTTACGTGGG 
CAGATCCGTG GCCTTCTCCA GAAAATCGAC ATGGATGGCA AAATGAAGAA AATGGCCGAG 
CTTCAGCTGT CCGTCGTTGG GGACCCCAAA AACAGAAAAG CTATTGAAAA CCAGATTCAA 
CAATGGGAGC AGAATCTCGA GCGCTTCAAT ATGGACCTTT TCCGCATGCG ATGCTACCTG 
GCCAGTCTGC AAGGAGGCGA GCTCCCCAAC CCAAAGAGCC TCCTAGCCAC GGCCAGCCGC 
CGCACCAAAG CTGCACTAGC CCGATTGGGA ATCTTCTCCG TCTCCTCCTT CCATGCACTG 
ATCTGTTCCA GAGATGAGGC CACGATGCGA AAGCGCTCTC TGTCTCTCTC CCACAGAGGC 
CGCAGCAAGA AAGGGGTCTT TTCATCCCTT AAGGGCCTGG ATACTCTTAC CAAGCGAGGC 
CATGAAAAAA AGCATTCTCT CTCACAGGAC CTCAGTGAAT TGAGCTGCTG TTCTTACAGT 
GGTCTTCCGC AGGTCTTTGA CAGTGATGGC GGAGCGCAGC TAAATTCCGT TACACATTGT 
AGTTTTAAGC GGTTAGGCAG TTTGACGAAT GTTTACTCAA TTGTGCCACC TGAAGGCAGC 
CAGTGGGATT GCAGTCGAGA AAGCACAACA TGCATCTACA TGCCAAACTG CCAGACTGTT 
ACTGTTCTCC TGAAAAGTCA TCACACTGTC ATGGATGTGT TGGCTGAGAC CTGCATGCTC 
GTGGGCCTGG ACCCCAGCAC ACACTGTTTG AGGCTGAGAA GATTCACAGG AGACAAGATG 
GAGATCTCAA CACCATCAGC TCATGAGCTT CTGCAAGGCT TGCCATATGA CGAATTGGAA 
GTTAGCCAGC TGCAAGTCTA CACTCTGCAC ATGTCTAGAC CCAGTCTAAC TGGAGACTTT 
GGCTTTGCTG TTACAGGCTA TGTAGACAGC CTCAAAAACA GCCATATCAT TGTCAGCGAA 
GTTCTCCCTG ATGGGCTCGC TTTCAGCAAA GGTCTTAGGC CTGGTGATGA GATCGTGGTT 
CTGAACGGTT GTGAGGTGTC TTCTCTGGAC CTGACTCTCA TACAGACTTT ATTTAATGAG 
CAGAATCTGC AGCTGACTGC GAAACGTCAC CTGCCCTCCA TTCCACATGA TTCAGACACA 
GCAAACGTGC AGTCCCCGTA CAGAGATATC CATCAAAACC GCCAGAGAGC CAAGTCCTCA 
ACAGATGTGT CCTGTGTGAC AGAAACCTTG TCTGTAAATG ATGGTCTTCA AGATTCTTAC 
CACAACCATC GCCAAGTTCA TCACAGCAAG AGTGTGGACA CGGTGTGTTC GCTGTACCAC 
TCCTACCAGG AAGGCTCTGG AGGTCTGATG GAGAGCCAGG AGGAGCAAAC GGGGCCTGAA 
CCCGCTATCC TCAGGCCCTG TCCGCGTCAT ATGTCTGCCA CAGAGAGACT ACGCAAGGTC 
ATCCAGGAAT TAGTGGATAC AGAGAAATCC TACGTAAAGG ACCTCAACTG CTTGTTTGAA 
ATCTACCTGA AGCCCCTGCA AAAGGAGACC TTTCTCACTC AAGATGAGAT GGAAAGCCTA 
TTTGGCAGCC TTCCAGAGAT GCTGGACTTC CAGAAAGTTT TCCTGCAGAC CCTGGAAGAG 
AAGATTAGTT CCTCCCCCAA CTTCAACACG TTCGAGACCC CTGTCCAATT CAAGAAGCTG 
CTCTTCTCTC TGGGTGGCTC ATTTCTGTAT TATGCGGAGC ACTTCAAACT GTACAGCGGC 
TTCTGCGCCA ACCATATTAA AGTGCAGAAG GTTCTGGAAC GAGCTAAAAC AGATCGATCT 
TTTAAAGAGT TCCTCGATGC TAGGAACCCG ACCAAGCAGC ACTCAACCAC GCTAGAGTCC 
TACCTGATCA AACCAGTGCA GAGAGTGCTG AAATACCCCC TGCTGCTCCG CGAGCTGGTG 
TCTCTGACGG ACACGGACAG TGAGGAGCAC TACCACCTCA CAGAGGCGCT TAAGGAAATG 
GAAAAGGTCG CAAGCCATAT AAACGAAATG CAGAAGATCT ACGAAGACTA CGGTGCAGTG 
TTTGACCAGC TGGTGGCAGA GCAAAGTGGC ACAGAGAAAG AGGTCACAGA GATATCTATG 
GGACAGTTCC TCATGCATTC CTCTGTGATC TGGCTCAACC CGTTCCCATC GTTGGGCCGT 
ATGAAAAAGG ATCCCGAGCT GACTGTTTTT GTTTTTAAGA GAGCCGTGAT ACTGCTGTAC 
CGGGAGAACA ACAAGGTGAG GAAGAAGATG GTGACTTCAA GATCAGCCCA CGGAGATCAG 
GATTCATTCA AGTTTCGCTG GCTCATTCCC CTGTCAGCGC TACAGGTCAG ACTGGGCAAC 
ACTGCAGACA CAGACTGCCT CTGGGAGCTG ATTCACACCA AATCACAAAA GGAGGGCAGG 
CCTGAGACGG TGTTCCAGTT GTGCAGCAGC ACACCAGAGT GCAAAGTGAA TATCATCAAG 
GTGATGCGCT CCATCTTGCG GGAGAACCTG CGTCGAAACA TGCAAAGGGT TGAACAGTCT 
GAGAGAACCT GTAAAAAGCG GCTTACTCCC CTTCGGAAAA GCCTGCCAGC TTCGGCTAGG 
ATAGGTTCTT CTCGAGCCTT TTGGCTAGCC AAACAACCTT CAAACGACAG TCCCGTTCCC 
AGCAATGATC CACTGAGGCT GTCTCTAGCA GATTCTGATT CAGCCAGTGC TAGCAGTGGA 
ACCACCAGCA GTGCCAGCGC TCCTCTAGAT CCGCCATCAC AGCAGCAGAG CGGCCTCAGA 
GAATCCAGCA TAATCAGCGA TGAGGACGAA GACGGCAGCG AGCCGGCTGA TTGGACGGAT 
GCTCCCAATG CTATCGAGGT CCAATTTCGC AAGCTTGGTC TGACCGAGGA GACCTCCCAG 
GCAGCTGCTC CACGTGTGCA GCCGGAGAGC GGAGAAGTGG ACACTCCAGA AAGGAAGGCT 
TCTCTAAAGC GAGCTCACTT TGCTGTGGTC AAACGCAAGC CCGCCAGAGA CACGAATAGC 
CTCAGACGCA GTCAGGTGGC CCTGCTGAAC ATGCGTAGAC ACAGCCACTC TTTGGACATC 
CCAGCGGAGA TCGCGGCACA TAGTGTAGAC CTGAACTCGC TACTGGAGAA AGATTTCAGC 
GTGCAGAGCC TGACCTCTGT TGTCAACGAA GACTTCTTCT ATGACAATCC CATGGAGCCA 
AAGAATGTGA CAACTTCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291563.1
CDS	547..5607
Translation	MGNSESQYRIQGTRSNSFIIPDREKPCSFSVHSSKEDVLSPLGFWNAGSRFSRLKSGNEGETSSTSKSSRAFISRQYDFITQKLKSGSQWDEVPEDLLPGGCLMLPSEKDCLYRNHKERRKTCNKYALNAMKTPERSLRLEDIEDQSSPRVVIKKDGSVRVEFNNSPNSALILNNCTEPVQLLKFSPTMESTCGLSAAGPSLDSHNGAAQAMSSLKSSKGSSLSSDGSLYDLPWRTSVELNDSDNASPDQQRQTVSTNTGFFDEQLPAITTTDLYRDPRIASTFPTVKDLQCSNGSRSHHRSSFVSVMEGLSSEGVPEEQQYSSFTLPCRKSKASPENNGKKASIRSRFRRISDWTGSLTRKKKFQEPRYNEPVDCFDSGVDGLTADTSSPSQVSSLMATSNGMSQSCSLSRSLHSRSGSAGALNLGNDILRQNIYKNFMMELKTARLPAERGAEDWCESSSEETGSSSQEIPGSLEQLDLLFEKEQGVVRRAGWLSFKPLLTVHKDRKLELVSRRKWKQFWVTLKGCTLLFYETYGKASSEPESPCYALFAEDGVVQAVPEHPKKENIFCLSNTYGDVYLFQASSQTDLENWVTAIHSASASLFAKRYGKEDTLRLLRGQIRGLLQKIDMDGKMKKMAELQLSVVGDPKNRKAIENQIQQWEQNLERFNMDLFRMRCYLASLQGGELPNPKSLLATASRRTKAALARLGIFSVSSFHALICSRDEATMRKRSLSLSHRGRSKKGVFSSLKGLDTLTKRGHEKKHSLSQDLSELSCCSYSGLPQVFDSDGGAQLNSVTHCSFKRLGSLTNVYSIVPPEGSQWDCSRESTTCIYMPNCQTVTVLLKSHHTVMDVLAETCMLVGLDPSTHCLRLRRFTGDKMEISTPSAHELLQGLPYDELEVSQLQVYTLHMSRPSLTGDFGFAVTGYVDSLKNSHIIVSEVLPDGLAFSKGLRPGDEIVVLNGCEVSSLDLTLIQTLFNEQNLQLTAKRHLPSIPHDSDTANVQSPYRDIHQNRQRAKSSTDVSCVTETLSVNDGLQDSYHNHRQVHHSKSVDTVCSLYHSYQEGSGGLMESQEEQTGPEPAILRPCPRHMSATERLRKVIQELVDTEKSYVKDLNCLFEIYLKPLQKETFLTQDEMESLFGSLPEMLDFQKVFLQTLEEKISSSPNFNTFETPVQFKKLLFSLGGSFLYYAEHFKLYSGFCANHIKVQKVLERAKTDRSFKEFLDARNPTKQHSTTLESYLIKPVQRVLKYPLLLRELVSLTDTDSEEHYHLTEALKEMEKVASHINEMQKIYEDYGAVFDQLVAEQSGTEKEVTEISMGQFLMHSSVIWLNPFPSLGRMKKDPELTVFVFKRAVILLYRENNKVRKKMVTSRSAHGDQDSFKFRWLIPLSALQVRLGNTADTDCLWELIHTKSQKEGRPETVFQLCSSTPECKVNIIKVMRSILRENLRRNMQRVEQSERTCKKRLTPLRKSLPASARIGSSRAFWLAKQPSNDSPVPSNDPLRLSLADSDSASASSGTTSSASAPLDPPSQQQSGLRESSIISDEDEDGSEPADWTDAPNAIEVQFRKLGLTEETSQAAAPRVQPESGEVDTPERKASLKRAHFAVVKRKPARDTNSLRRSQVALLNMRRHSHSLDIPAEIAAHSVDLNSLLEKDFSVQSLTSVVNEDFFYDNPMEPKNVTTS

Target ORF information:

RefSeq Version	XM_009293288.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio T-cell lymphoma invasion and metastasis 2a (tiam2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293288.2

ORF Insert Sequence:

ATGGGAAACT CGGAGAGTCA GTATAGGATC CAGGGCACTA GAAGCAATAG CTTTATTATC 
CCTGACCGAG AGAAACCCTG TTCATTCAGC GTCCACTCCA GCAAAGAAGA CGTTTTATCA 
CCTTTAGGCT TTTGGAATGC TGGTTCAAGA TTTTCTAGAT TGAAATCCGG AAATGAAGGT 
GAAACTTCCT CCACGTCTAA GAGCAGCCGG GCGTTTATAT CCCGCCAGTA TGACTTCATC 
ACCCAGAAAC TAAAGAGTGG TAGTCAGTGG GATGAAGTTC CTGAGGACCT GTTGCCTGGT 
GGCTGCTTGA TGTTGCCTTC GGAAAAGGAC TGCTTGTATC GCAATCACAA GGAGAGGAGA 
AAAACATGTA ATAAATATGC TCTTAATGCA ATGAAGACAC CTGAGAGAAG TCTCAGACTT 
GAGGACATTG AAGACCAGAG CAGCCCTAGG GTTGTCATAA AGAAAGATGG TAGCGTACGT 
GTGGAATTTA ACAATAGTCC CAATAGTGCC CTTATTTTAA ATAATTGCAC TGAACCAGTT 
CAGCTCCTCA AATTTTCCCC CACCATGGAG TCCACTTGTG GTTTATCTGC TGCAGGACCC 
TCATTGGATT CTCATAATGG AGCAGCACAG GCAATGTCCA GCTTGAAAAG CAGCAAAGGT 
AGCTCTCTGA GCTCAGATGG CTCTTTGTAC GACTTGCCTT GGAGGACCAG CGTCGAACTT 
AATGATTCAG ACAATGCTTC TCCTGACCAG CAAAGGCAAA CTGTTTCTAC AAACACAGGC 
TTTTTTGATG AGCAGTTGCC AGCCATTACT ACAACCGACC TCTACAGGGA TCCCAGAATT 
GCTTCCACCT TCCCCACGGT CAAGGATTTG CAGTGTTCAA ATGGCAGCCG TTCACACCAC 
AGATCCTCCT TTGTGTCCGT GATGGAGGGA CTCAGTTCAG AAGGGGTCCC AGAGGAACAG 
CAGTATTCCT CCTTCACCTT GCCTTGCCGG AAATCCAAAG CTTCTCCTGA AAATAATGGA 
AAGAAAGCGT CGATTAGGAG CAGATTTAGA CGCATCAGTG ACTGGACCGG AAGTCTGACC 
AGGAAAAAGA AGTTTCAGGA GCCCAGATAT AACGAGCCGG TCGATTGCTT TGACAGCGGT 
GTGGATGGAC TCACTGCAGA TACCAGTTCC CCCTCTCAGG TCTCCAGCCT TATGGCTACC 
TCCAATGGCA TGTCTCAATC CTGCTCTCTC TCTCGCTCGC TGCACAGCCG CAGTGGCTCT 
GCCGGAGCCC TTAATTTAGG AAATGACATT CTACGGCAAA ACATCTACAA GAATTTTATG 
ATGGAGTTAA AGACTGCCCG GCTTCCAGCA GAAAGAGGAG CTGAAGATTG GTGTGAGAGC 
TCCTCAGAGG AAACAGGAAG CAGTAGTCAA GAAATCCCGG GGTCTCTGGA GCAGCTGGAT 
TTGCTGTTCG AGAAAGAACA AGGGGTAGTT AGGCGAGCTG GGTGGCTGTC CTTCAAGCCC 
TTGCTCACTG TACACAAGGA CCGCAAACTG GAGCTAGTGA GCCGTCGAAA ATGGAAGCAA 
TTCTGGGTCA CTCTCAAGGG TTGCACACTG CTGTTTTATG AAACCTATGG AAAGGCATCT 
TCAGAGCCAG AATCTCCATG CTATGCCTTG TTCGCAGAAG ATGGCGTAGT CCAGGCTGTT 
CCCGAACACC CTAAGAAGGA GAATATCTTC TGCCTTAGTA ACACCTATGG CGATGTCTAC 
CTTTTCCAGG CCTCCAGTCA GACTGACCTA GAGAACTGGG TTACTGCCAT TCATTCAGCA 
AGTGCCTCTT TGTTTGCGAA ACGCTACGGC AAAGAGGACA CACTGCGATT GTTACGTGGG 
CAGATCCGTG GCCTTCTCCA GAAAATCGAC ATGGATGGCA AAATGAAGAA AATGGCCGAG 
CTTCAGCTGT CCGTCGTTGG GGACCCCAAA AACAGAAAAG CTATTGAAAA CCAGATTCAA 
CAATGGGAGC AGAATCTCGA GCGCTTCAAT ATGGACCTTT TCCGCATGCG ATGCTACCTG 
GCCAGTCTGC AAGGAGGCGA GCTCCCCAAC CCAAAGAGCC TCCTAGCCAC GGCCAGCCGC 
CGCACCAAAG CTGCACTAGC CCGATTGGGA ATCTTCTCCG TCTCCTCCTT CCATGCACTG 
ATCTGTTCCA GAGATGAGGC CACGATGCGA AAGCGCTCTC TGTCTCTCTC CCACAGAGGC 
CGCAGCAAGA AAGGGGTCTT TTCATCCCTT AAGGGCCTGG ATACTCTTAC CAAGCGAGGC 
CATGAAAAAA AGCATTCTCT CTCACAGGAC CTCAGTGAAT TGAGCTGCTG TTCTTACAGT 
GGTCTTCCGC AGGTCTTTGA CAGTGATGGC GGAGCGCAGC TAAATTCCGT TACACATTGT 
AGTTTTAAGC GGTTAGGCAG TTTGACGAAT GTTTACTCAA TTGTGCCACC TGAAGGCAGC 
CAGTGGGATT GCAGTCGAGA AAGCACAACA TGCATCTACA TGCCAAACTG CCAGACTGTT 
ACTGTTCTCC TGAAAAGTCA TCACACTGTC ATGGATGTGT TGGCTGAGAC CTGCATGCTC 
GTGGGCCTGG ACCCCAGCAC ACACTGTTTG AGGCTGAGAA GATTCACAGG AGACAAGATG 
GAGATCTCAA CACCATCAGC TCATGAGCTT CTGCAAGGCT TGCCATATGA CGAATTGGAA 
GTTAGCCAGC TGCAAGTCTA CACTCTGCAC ATGTCTAGAC CCAGTCTAAC TGGAGACTTT 
GGCTTTGCTG TTACAGGCTA TGTAGACAGC CTCAAAAACA GCCATATCAT TGTCAGCGAA 
GTTCTCCCTG ATGGGCTCGC TTTCAGCAAA GGTCTTAGGC CTGGTGATGA GATCGTGGTT 
CTGAACGGTT GTGAGGTGTC TTCTCTGGAC CTGACTCTCA TACAGACTTT ATTTAATGAG 
CAGAATCTGC AGCTGACTGC GAAACGTCAC CTGCCCTCCA TTCCACATGA TTCAGACACA 
GCAAACGTGC AGTCCCCGTA CAGAGATATC CATCAAAACC GCCAGAGAGC CAAGTCCTCA 
ACAGATGTGT CCTGTGTGAC AGAAACCTTG TCTGTAAATG ATGGTCTTCA AGATTCTTAC 
CACAACCATC GCCAAGTTCA TCACAGCAAG AGTGTGGACA CGGTGTGTTC GCTGTACCAC 
TCCTACCAGG AAGGCTCTGG AGGTCTGATG GAGAGCCAGG AGGAGCAAAC GGGGCCTGAA 
CCCGCTATCC TCAGGCCCTG TCCGCGTCAT ATGTCTGCCA CAGAGAGACT ACGCAAGGTC 
ATCCAGGAAT TAGTGGATAC AGAGAAATCC TACGTAAAGG ACCTCAACTG CTTGTTTGAA 
ATCTACCTGA AGCCCCTGCA AAAGGAGACC TTTCTCACTC AAGATGAGAT GGAAAGCCTA 
TTTGGCAGCC TTCCAGAGAT GCTGGACTTC CAGAAAGTTT TCCTGCAGAC CCTGGAAGAG 
AAGATTAGTT CCTCCCCCAA CTTCAACACG TTCGAGACCC CTGTCCAATT CAAGAAGCTG 
CTCTTCTCTC TGGGTGGCTC ATTTCTGTAT TATGCGGAGC ACTTCAAACT GTACAGCGGC 
TTCTGCGCCA ACCATATTAA AGTGCAGAAG GTTCTGGAAC GAGCTAAAAC AGATCGATCT 
TTTAAAGAGT TCCTCGATGC TAGGAACCCG ACCAAGCAGC ACTCAACCAC GCTAGAGTCC 
TACCTGATCA AACCAGTGCA GAGAGTGCTG AAATACCCCC TGCTGCTCCG CGAGCTGGTG 
TCTCTGACGG ACACGGACAG TGAGGAGCAC TACCACCTCA CAGAGGCGCT TAAGGAAATG 
GAAAAGGTCG CAAGCCATAT AAACGAAATG CAGAAGATCT ACGAAGACTA CGGTGCAGTG 
TTTGACCAGC TGGTGGCAGA GCAAAGTGGC ACAGAGAAAG AGGTCACAGA GATATCTATG 
GGACAGTTCC TCATGCATTC CTCTGTGATC TGGCTCAACC CGTTCCCATC GTTGGGCCGT 
ATGAAAAAGG ATCCCGAGCT GACTGTTTTT GTTTTTAAGA GAGCCGTGAT ACTGCTGTAC 
CGGGAGAACA ACAAGGTGAG GAAGAAGATG GTGACTTCAA GATCAGCCCA CGGAGATCAG 
GATTCATTCA AGTTTCGCTG GCTCATTCCC CTGTCAGCGC TACAGGTCAG ACTGGGCAAC 
ACTGCAGACA CAGACTGCCT CTGGGAGCTG ATTCACACCA AATCACAAAA GGAGGGCAGG 
CCTGAGACGG TGTTCCAGTT GTGCAGCAGC ACACCAGAGT GCAAAGTGAA TATCATCAAG 
GTGATGCGCT CCATCTTGCG GGAGAACCTG CGTCGAAACA TGCAAAGGGT TGAACAGTCT 
GAGAGAACCT GTAAAAAGCG GCTTACTCCC CTTCGGAAAA GCCTGCCAGC TTCGGCTAGG 
ATAGGTTCTT CTCGAGCCTT TTGGCTAGCC AAACAACCTT CAAACGACAG TCCCGTTCCC 
AGCAATGATC CACTGAGGCT GTCTCTAGCA GATTCTGATT CAGCCAGTGC TAGCAGTGGA 
ACCACCAGCA GTGCCAGCGC TCCTCTAGAT CCGCCATCAC AGCAGCAGAG CGGCCTCAGA 
GAATCCAGCA TAATCAGCGA TGAGGACGAA GACGGCAGCG AGCCGGCTGA TTGGACGGAT 
GCTCCCAATG CTATCGAGGT CCAATTTCGC AAGCTTGGTC TGACCGAGGA GACCTCCCAG 
GCAGCTGCTC CACGTGTGCA GCCGGAGAGC GGAGAAGTGG ACACTCCAGA AAGGAAGGCT 
TCTCTAAAGC GAGCTCACTT TGCTGTGGTC AAACGCAAGC CCGCCAGAGA CACGAATAGC 
CTCAGACGCA GTCAGGTGGC CCTGCTGAAC ATGCGTAGAC ACAGCCACTC TTTGGACATC 
CCAGCGGAGA TCGCGGCACA TAGTGTAGAC CTGAACTCGC TACTGGAGAA AGATTTCAGC 
GTGCAGAGCC TGACCTCTGT TGTCAACGAA GACTTCTTCT ATGACAATCC CATGGAGCCA 
AAGAATGTGA CAACTTCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tiam2a ( XM_009293288.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009291563.1 Danio rerio T-cell lymphoma invasion and metastasis 2a (tiam2a), transcript variant X1, mRNA.

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CloneID	ODa49964
Clone ID Related Accession (Same CDS sequence)	XM_021467480.1 , XM_009293288.2 , XM_009293288.3
Accession Version	XM_009293288.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	T-lymphoma invasion and metastasis-inducing protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293288.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAACT CGGAGAGTCA GTATAGGATC CAGGGCACTA GAAGCAATAG CTTTATTATC 
CCTGACCGAG AGAAACCCTG TTCATTCAGC GTCCACTCCA GCAAAGAAGA CGTTTTATCA 
CCTTTAGGCT TTTGGAATGC TGGTTCAAGA TTTTCTAGAT TGAAATCCGG AAATGAAGGT 
GAAACTTCCT CCACGTCTAA GAGCAGCCGG GCGTTTATAT CCCGCCAGTA TGACTTCATC 
ACCCAGAAAC TAAAGAGTGG TAGTCAGTGG GATGAAGTTC CTGAGGACCT GTTGCCTGGT 
GGCTGCTTGA TGTTGCCTTC GGAAAAGGAC TGCTTGTATC GCAATCACAA GGAGAGGAGA 
AAAACATGTA ATAAATATGC TCTTAATGCA ATGAAGACAC CTGAGAGAAG TCTCAGACTT 
GAGGACATTG AAGACCAGAG CAGCCCTAGG GTTGTCATAA AGAAAGATGG TAGCGTACGT 
GTGGAATTTA ACAATAGTCC CAATAGTGCC CTTATTTTAA ATAATTGCAC TGAACCAGTT 
CAGCTCCTCA AATTTTCCCC CACCATGGAG TCCACTTGTG GTTTATCTGC TGCAGGACCC 
TCATTGGATT CTCATAATGG AGCAGCACAG GCAATGTCCA GCTTGAAAAG CAGCAAAGGT 
AGCTCTCTGA GCTCAGATGG CTCTTTGTAC GACTTGCCTT GGAGGACCAG CGTCGAACTT 
AATGATTCAG ACAATGCTTC TCCTGACCAG CAAAGGCAAA CTGTTTCTAC AAACACAGGC 
TTTTTTGATG AGCAGTTGCC AGCCATTACT ACAACCGACC TCTACAGGGA TCCCAGAATT 
GCTTCCACCT TCCCCACGGT CAAGGATTTG CAGTGTTCAA ATGGCAGCCG TTCACACCAC 
AGATCCTCCT TTGTGTCCGT GATGGAGGGA CTCAGTTCAG AAGGGGTCCC AGAGGAACAG 
CAGTATTCCT CCTTCACCTT GCCTTGCCGG AAATCCAAAG CTTCTCCTGA AAATAATGGA 
AAGAAAGCGT CGATTAGGAG CAGATTTAGA CGCATCAGTG ACTGGACCGG AAGTCTGACC 
AGGAAAAAGA AGTTTCAGGA GCCCAGATAT AACGAGCCGG TCGATTGCTT TGACAGCGGT 
GTGGATGGAC TCACTGCAGA TACCAGTTCC CCCTCTCAGG TCTCCAGCCT TATGGCTACC 
TCCAATGGCA TGTCTCAATC CTGCTCTCTC TCTCGCTCGC TGCACAGCCG CAGTGGCTCT 
GCCGGAGCCC TTAATTTAGG AAATGACATT CTACGGCAAA ACATCTACAA GAATTTTATG 
ATGGAGTTAA AGACTGCCCG GCTTCCAGCA GAAAGAGGAG CTGAAGATTG GTGTGAGAGC 
TCCTCAGAGG AAACAGGAAG CAGTAGTCAA GAAATCCCGG GGTCTCTGGA GCAGCTGGAT 
TTGCTGTTCG AGAAAGAACA AGGGGTAGTT AGGCGAGCTG GGTGGCTGTC CTTCAAGCCC 
TTGCTCACTG TACACAAGGA CCGCAAACTG GAGCTAGTGA GCCGTCGAAA ATGGAAGCAA 
TTCTGGGTCA CTCTCAAGGG TTGCACACTG CTGTTTTATG AAACCTATGG AAAGGCATCT 
TCAGAGCCAG AATCTCCATG CTATGCCTTG TTCGCAGAAG ATGGCGTAGT CCAGGCTGTT 
CCCGAACACC CTAAGAAGGA GAATATCTTC TGCCTTAGTA ACACCTATGG CGATGTCTAC 
CTTTTCCAGG CCTCCAGTCA GACTGACCTA GAGAACTGGG TTACTGCCAT TCATTCAGCA 
AGTGCCTCTT TGTTTGCGAA ACGCTACGGC AAAGAGGACA CACTGCGATT GTTACGTGGG 
CAGATCCGTG GCCTTCTCCA GAAAATCGAC ATGGATGGCA AAATGAAGAA AATGGCCGAG 
CTTCAGCTGT CCGTCGTTGG GGACCCCAAA AACAGAAAAG CTATTGAAAA CCAGATTCAA 
CAATGGGAGC AGAATCTCGA GCGCTTCAAT ATGGACCTTT TCCGCATGCG ATGCTACCTG 
GCCAGTCTGC AAGGAGGCGA GCTCCCCAAC CCAAAGAGCC TCCTAGCCAC GGCCAGCCGC 
CGCACCAAAG CTGCACTAGC CCGATTGGGA ATCTTCTCCG TCTCCTCCTT CCATGCACTG 
ATCTGTTCCA GAGATGAGGC CACGATGCGA AAGCGCTCTC TGTCTCTCTC CCACAGAGGC 
CGCAGCAAGA AAGGGGTCTT TTCATCCCTT AAGGGCCTGG ATACTCTTAC CAAGCGAGGC 
CATGAAAAAA AGCATTCTCT CTCACAGGAC CTCAGTGAAT TGAGCTGCTG TTCTTACAGT 
GGTCTTCCGC AGGTCTTTGA CAGTGATGGC GGAGCGCAGC TAAATTCCGT TACACATTGT 
AGTTTTAAGC GGTTAGGCAG TTTGACGAAT GTTTACTCAA TTGTGCCACC TGAAGGCAGC 
CAGTGGGATT GCAGTCGAGA AAGCACAACA TGCATCTACA TGCCAAACTG CCAGACTGTT 
ACTGTTCTCC TGAAAAGTCA TCACACTGTC ATGGATGTGT TGGCTGAGAC CTGCATGCTC 
GTGGGCCTGG ACCCCAGCAC ACACTGTTTG AGGCTGAGAA GATTCACAGG AGACAAGATG 
GAGATCTCAA CACCATCAGC TCATGAGCTT CTGCAAGGCT TGCCATATGA CGAATTGGAA 
GTTAGCCAGC TGCAAGTCTA CACTCTGCAC ATGTCTAGAC CCAGTCTAAC TGGAGACTTT 
GGCTTTGCTG TTACAGGCTA TGTAGACAGC CTCAAAAACA GCCATATCAT TGTCAGCGAA 
GTTCTCCCTG ATGGGCTCGC TTTCAGCAAA GGTCTTAGGC CTGGTGATGA GATCGTGGTT 
CTGAACGGTT GTGAGGTGTC TTCTCTGGAC CTGACTCTCA TACAGACTTT ATTTAATGAG 
CAGAATCTGC AGCTGACTGC GAAACGTCAC CTGCCCTCCA TTCCACATGA TTCAGACACA 
GCAAACGTGC AGTCCCCGTA CAGAGATATC CATCAAAACC GCCAGAGAGC CAAGTCCTCA 
ACAGATGTGT CCTGTGTGAC AGAAACCTTG TCTGTAAATG ATGGTCTTCA AGATTCTTAC 
CACAACCATC GCCAAGTTCA TCACAGCAAG AGTGTGGACA CGGTGTGTTC GCTGTACCAC 
TCCTACCAGG AAGGCTCTGG AGGTCTGATG GAGAGCCAGG AGGAGCAAAC GGGGCCTGAA 
CCCGCTATCC TCAGGCCCTG TCCGCGTCAT ATGTCTGCCA CAGAGAGACT ACGCAAGGTC 
ATCCAGGAAT TAGTGGATAC AGAGAAATCC TACGTAAAGG ACCTCAACTG CTTGTTTGAA 
ATCTACCTGA AGCCCCTGCA AAAGGAGACC TTTCTCACTC AAGATGAGAT GGAAAGCCTA 
TTTGGCAGCC TTCCAGAGAT GCTGGACTTC CAGAAAGTTT TCCTGCAGAC CCTGGAAGAG 
AAGATTAGTT CCTCCCCCAA CTTCAACACG TTCGAGACCC CTGTCCAATT CAAGAAGCTG 
CTCTTCTCTC TGGGTGGCTC ATTTCTGTAT TATGCGGAGC ACTTCAAACT GTACAGCGGC 
TTCTGCGCCA ACCATATTAA AGTGCAGAAG GTTCTGGAAC GAGCTAAAAC AGATCGATCT 
TTTAAAGAGT TCCTCGATGC TAGGAACCCG ACCAAGCAGC ACTCAACCAC GCTAGAGTCC 
TACCTGATCA AACCAGTGCA GAGAGTGCTG AAATACCCCC TGCTGCTCCG CGAGCTGGTG 
TCTCTGACGG ACACGGACAG TGAGGAGCAC TACCACCTCA CAGAGGCGCT TAAGGAAATG 
GAAAAGGTCG CAAGCCATAT AAACGAAATG CAGAAGATCT ACGAAGACTA CGGTGCAGTG 
TTTGACCAGC TGGTGGCAGA GCAAAGTGGC ACAGAGAAAG AGGTCACAGA GATATCTATG 
GGACAGTTCC TCATGCATTC CTCTGTGATC TGGCTCAACC CGTTCCCATC GTTGGGCCGT 
ATGAAAAAGG ATCCCGAGCT GACTGTTTTT GTTTTTAAGA GAGCCGTGAT ACTGCTGTAC 
CGGGAGAACA ACAAGGTGAG GAAGAAGATG GTGACTTCAA GATCAGCCCA CGGAGATCAG 
GATTCATTCA AGTTTCGCTG GCTCATTCCC CTGTCAGCGC TACAGGTCAG ACTGGGCAAC 
ACTGCAGACA CAGACTGCCT CTGGGAGCTG ATTCACACCA AATCACAAAA GGAGGGCAGG 
CCTGAGACGG TGTTCCAGTT GTGCAGCAGC ACACCAGAGT GCAAAGTGAA TATCATCAAG 
GTGATGCGCT CCATCTTGCG GGAGAACCTG CGTCGAAACA TGCAAAGGGT TGAACAGTCT 
GAGAGAACCT GTAAAAAGCG GCTTACTCCC CTTCGGAAAA GCCTGCCAGC TTCGGCTAGG 
ATAGGTTCTT CTCGAGCCTT TTGGCTAGCC AAACAACCTT CAAACGACAG TCCCGTTCCC 
AGCAATGATC CACTGAGGCT GTCTCTAGCA GATTCTGATT CAGCCAGTGC TAGCAGTGGA 
ACCACCAGCA GTGCCAGCGC TCCTCTAGAT CCGCCATCAC AGCAGCAGAG CGGCCTCAGA 
GAATCCAGCA TAATCAGCGA TGAGGACGAA GACGGCAGCG AGCCGGCTGA TTGGACGGAT 
GCTCCCAATG CTATCGAGGT CCAATTTCGC AAGCTTGGTC TGACCGAGGA GACCTCCCAG 
GCAGCTGCTC CACGTGTGCA GCCGGAGAGC GGAGAAGTGG ACACTCCAGA AAGGAAGGCT 
TCTCTAAAGC GAGCTCACTT TGCTGTGGTC AAACGCAAGC CCGCCAGAGA CACGAATAGC 
CTCAGACGCA GTCAGGTGGC CCTGCTGAAC ATGCGTAGAC ACAGCCACTC TTTGGACATC 
CCAGCGGAGA TCGCGGCACA TAGTGTAGAC CTGAACTCGC TACTGGAGAA AGATTTCAGC 
GTGCAGAGCC TGACCTCTGT TGTCAACGAA GACTTCTTCT ATGACAATCC CATGGAGCCA 
AAGAATGTGA CAACTTCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291563.1
CDS	489..5549
Translation	MGNSESQYRIQGTRSNSFIIPDREKPCSFSVHSSKEDVLSPLGFWNAGSRFSRLKSGNEGETSSTSKSSRAFISRQYDFITQKLKSGSQWDEVPEDLLPGGCLMLPSEKDCLYRNHKERRKTCNKYALNAMKTPERSLRLEDIEDQSSPRVVIKKDGSVRVEFNNSPNSALILNNCTEPVQLLKFSPTMESTCGLSAAGPSLDSHNGAAQAMSSLKSSKGSSLSSDGSLYDLPWRTSVELNDSDNASPDQQRQTVSTNTGFFDEQLPAITTTDLYRDPRIASTFPTVKDLQCSNGSRSHHRSSFVSVMEGLSSEGVPEEQQYSSFTLPCRKSKASPENNGKKASIRSRFRRISDWTGSLTRKKKFQEPRYNEPVDCFDSGVDGLTADTSSPSQVSSLMATSNGMSQSCSLSRSLHSRSGSAGALNLGNDILRQNIYKNFMMELKTARLPAERGAEDWCESSSEETGSSSQEIPGSLEQLDLLFEKEQGVVRRAGWLSFKPLLTVHKDRKLELVSRRKWKQFWVTLKGCTLLFYETYGKASSEPESPCYALFAEDGVVQAVPEHPKKENIFCLSNTYGDVYLFQASSQTDLENWVTAIHSASASLFAKRYGKEDTLRLLRGQIRGLLQKIDMDGKMKKMAELQLSVVGDPKNRKAIENQIQQWEQNLERFNMDLFRMRCYLASLQGGELPNPKSLLATASRRTKAALARLGIFSVSSFHALICSRDEATMRKRSLSLSHRGRSKKGVFSSLKGLDTLTKRGHEKKHSLSQDLSELSCCSYSGLPQVFDSDGGAQLNSVTHCSFKRLGSLTNVYSIVPPEGSQWDCSRESTTCIYMPNCQTVTVLLKSHHTVMDVLAETCMLVGLDPSTHCLRLRRFTGDKMEISTPSAHELLQGLPYDELEVSQLQVYTLHMSRPSLTGDFGFAVTGYVDSLKNSHIIVSEVLPDGLAFSKGLRPGDEIVVLNGCEVSSLDLTLIQTLFNEQNLQLTAKRHLPSIPHDSDTANVQSPYRDIHQNRQRAKSSTDVSCVTETLSVNDGLQDSYHNHRQVHHSKSVDTVCSLYHSYQEGSGGLMESQEEQTGPEPAILRPCPRHMSATERLRKVIQELVDTEKSYVKDLNCLFEIYLKPLQKETFLTQDEMESLFGSLPEMLDFQKVFLQTLEEKISSSPNFNTFETPVQFKKLLFSLGGSFLYYAEHFKLYSGFCANHIKVQKVLERAKTDRSFKEFLDARNPTKQHSTTLESYLIKPVQRVLKYPLLLRELVSLTDTDSEEHYHLTEALKEMEKVASHINEMQKIYEDYGAVFDQLVAEQSGTEKEVTEISMGQFLMHSSVIWLNPFPSLGRMKKDPELTVFVFKRAVILLYRENNKVRKKMVTSRSAHGDQDSFKFRWLIPLSALQVRLGNTADTDCLWELIHTKSQKEGRPETVFQLCSSTPECKVNIIKVMRSILRENLRRNMQRVEQSERTCKKRLTPLRKSLPASARIGSSRAFWLAKQPSNDSPVPSNDPLRLSLADSDSASASSGTTSSASAPLDPPSQQQSGLRESSIISDEDEDGSEPADWTDAPNAIEVQFRKLGLTEETSQAAAPRVQPESGEVDTPERKASLKRAHFAVVKRKPARDTNSLRRSQVALLNMRRHSHSLDIPAEIAAHSVDLNSLLEKDFSVQSLTSVVNEDFFYDNPMEPKNVTTS

Target ORF information:

RefSeq Version	XM_009293288.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio T-cell lymphoma invasion and metastasis 2a (tiam2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293288.3

ORF Insert Sequence:

ATGGGAAACT CGGAGAGTCA GTATAGGATC CAGGGCACTA GAAGCAATAG CTTTATTATC 
CCTGACCGAG AGAAACCCTG TTCATTCAGC GTCCACTCCA GCAAAGAAGA CGTTTTATCA 
CCTTTAGGCT TTTGGAATGC TGGTTCAAGA TTTTCTAGAT TGAAATCCGG AAATGAAGGT 
GAAACTTCCT CCACGTCTAA GAGCAGCCGG GCGTTTATAT CCCGCCAGTA TGACTTCATC 
ACCCAGAAAC TAAAGAGTGG TAGTCAGTGG GATGAAGTTC CTGAGGACCT GTTGCCTGGT 
GGCTGCTTGA TGTTGCCTTC GGAAAAGGAC TGCTTGTATC GCAATCACAA GGAGAGGAGA 
AAAACATGTA ATAAATATGC TCTTAATGCA ATGAAGACAC CTGAGAGAAG TCTCAGACTT 
GAGGACATTG AAGACCAGAG CAGCCCTAGG GTTGTCATAA AGAAAGATGG TAGCGTACGT 
GTGGAATTTA ACAATAGTCC CAATAGTGCC CTTATTTTAA ATAATTGCAC TGAACCAGTT 
CAGCTCCTCA AATTTTCCCC CACCATGGAG TCCACTTGTG GTTTATCTGC TGCAGGACCC 
TCATTGGATT CTCATAATGG AGCAGCACAG GCAATGTCCA GCTTGAAAAG CAGCAAAGGT 
AGCTCTCTGA GCTCAGATGG CTCTTTGTAC GACTTGCCTT GGAGGACCAG CGTCGAACTT 
AATGATTCAG ACAATGCTTC TCCTGACCAG CAAAGGCAAA CTGTTTCTAC AAACACAGGC 
TTTTTTGATG AGCAGTTGCC AGCCATTACT ACAACCGACC TCTACAGGGA TCCCAGAATT 
GCTTCCACCT TCCCCACGGT CAAGGATTTG CAGTGTTCAA ATGGCAGCCG TTCACACCAC 
AGATCCTCCT TTGTGTCCGT GATGGAGGGA CTCAGTTCAG AAGGGGTCCC AGAGGAACAG 
CAGTATTCCT CCTTCACCTT GCCTTGCCGG AAATCCAAAG CTTCTCCTGA AAATAATGGA 
AAGAAAGCGT CGATTAGGAG CAGATTTAGA CGCATCAGTG ACTGGACCGG AAGTCTGACC 
AGGAAAAAGA AGTTTCAGGA GCCCAGATAT AACGAGCCGG TCGATTGCTT TGACAGCGGT 
GTGGATGGAC TCACTGCAGA TACCAGTTCC CCCTCTCAGG TCTCCAGCCT TATGGCTACC 
TCCAATGGCA TGTCTCAATC CTGCTCTCTC TCTCGCTCGC TGCACAGCCG CAGTGGCTCT 
GCCGGAGCCC TTAATTTAGG AAATGACATT CTACGGCAAA ACATCTACAA GAATTTTATG 
ATGGAGTTAA AGACTGCCCG GCTTCCAGCA GAAAGAGGAG CTGAAGATTG GTGTGAGAGC 
TCCTCAGAGG AAACAGGAAG CAGTAGTCAA GAAATCCCGG GGTCTCTGGA GCAGCTGGAT 
TTGCTGTTCG AGAAAGAACA AGGGGTAGTT AGGCGAGCTG GGTGGCTGTC CTTCAAGCCC 
TTGCTCACTG TACACAAGGA CCGCAAACTG GAGCTAGTGA GCCGTCGAAA ATGGAAGCAA 
TTCTGGGTCA CTCTCAAGGG TTGCACACTG CTGTTTTATG AAACCTATGG AAAGGCATCT 
TCAGAGCCAG AATCTCCATG CTATGCCTTG TTCGCAGAAG ATGGCGTAGT CCAGGCTGTT 
CCCGAACACC CTAAGAAGGA GAATATCTTC TGCCTTAGTA ACACCTATGG CGATGTCTAC 
CTTTTCCAGG CCTCCAGTCA GACTGACCTA GAGAACTGGG TTACTGCCAT TCATTCAGCA 
AGTGCCTCTT TGTTTGCGAA ACGCTACGGC AAAGAGGACA CACTGCGATT GTTACGTGGG 
CAGATCCGTG GCCTTCTCCA GAAAATCGAC ATGGATGGCA AAATGAAGAA AATGGCCGAG 
CTTCAGCTGT CCGTCGTTGG GGACCCCAAA AACAGAAAAG CTATTGAAAA CCAGATTCAA 
CAATGGGAGC AGAATCTCGA GCGCTTCAAT ATGGACCTTT TCCGCATGCG ATGCTACCTG 
GCCAGTCTGC AAGGAGGCGA GCTCCCCAAC CCAAAGAGCC TCCTAGCCAC GGCCAGCCGC 
CGCACCAAAG CTGCACTAGC CCGATTGGGA ATCTTCTCCG TCTCCTCCTT CCATGCACTG 
ATCTGTTCCA GAGATGAGGC CACGATGCGA AAGCGCTCTC TGTCTCTCTC CCACAGAGGC 
CGCAGCAAGA AAGGGGTCTT TTCATCCCTT AAGGGCCTGG ATACTCTTAC CAAGCGAGGC 
CATGAAAAAA AGCATTCTCT CTCACAGGAC CTCAGTGAAT TGAGCTGCTG TTCTTACAGT 
GGTCTTCCGC AGGTCTTTGA CAGTGATGGC GGAGCGCAGC TAAATTCCGT TACACATTGT 
AGTTTTAAGC GGTTAGGCAG TTTGACGAAT GTTTACTCAA TTGTGCCACC TGAAGGCAGC 
CAGTGGGATT GCAGTCGAGA AAGCACAACA TGCATCTACA TGCCAAACTG CCAGACTGTT 
ACTGTTCTCC TGAAAAGTCA TCACACTGTC ATGGATGTGT TGGCTGAGAC CTGCATGCTC 
GTGGGCCTGG ACCCCAGCAC ACACTGTTTG AGGCTGAGAA GATTCACAGG AGACAAGATG 
GAGATCTCAA CACCATCAGC TCATGAGCTT CTGCAAGGCT TGCCATATGA CGAATTGGAA 
GTTAGCCAGC TGCAAGTCTA CACTCTGCAC ATGTCTAGAC CCAGTCTAAC TGGAGACTTT 
GGCTTTGCTG TTACAGGCTA TGTAGACAGC CTCAAAAACA GCCATATCAT TGTCAGCGAA 
GTTCTCCCTG ATGGGCTCGC TTTCAGCAAA GGTCTTAGGC CTGGTGATGA GATCGTGGTT 
CTGAACGGTT GTGAGGTGTC TTCTCTGGAC CTGACTCTCA TACAGACTTT ATTTAATGAG 
CAGAATCTGC AGCTGACTGC GAAACGTCAC CTGCCCTCCA TTCCACATGA TTCAGACACA 
GCAAACGTGC AGTCCCCGTA CAGAGATATC CATCAAAACC GCCAGAGAGC CAAGTCCTCA 
ACAGATGTGT CCTGTGTGAC AGAAACCTTG TCTGTAAATG ATGGTCTTCA AGATTCTTAC 
CACAACCATC GCCAAGTTCA TCACAGCAAG AGTGTGGACA CGGTGTGTTC GCTGTACCAC 
TCCTACCAGG AAGGCTCTGG AGGTCTGATG GAGAGCCAGG AGGAGCAAAC GGGGCCTGAA 
CCCGCTATCC TCAGGCCCTG TCCGCGTCAT ATGTCTGCCA CAGAGAGACT ACGCAAGGTC 
ATCCAGGAAT TAGTGGATAC AGAGAAATCC TACGTAAAGG ACCTCAACTG CTTGTTTGAA 
ATCTACCTGA AGCCCCTGCA AAAGGAGACC TTTCTCACTC AAGATGAGAT GGAAAGCCTA 
TTTGGCAGCC TTCCAGAGAT GCTGGACTTC CAGAAAGTTT TCCTGCAGAC CCTGGAAGAG 
AAGATTAGTT CCTCCCCCAA CTTCAACACG TTCGAGACCC CTGTCCAATT CAAGAAGCTG 
CTCTTCTCTC TGGGTGGCTC ATTTCTGTAT TATGCGGAGC ACTTCAAACT GTACAGCGGC 
TTCTGCGCCA ACCATATTAA AGTGCAGAAG GTTCTGGAAC GAGCTAAAAC AGATCGATCT 
TTTAAAGAGT TCCTCGATGC TAGGAACCCG ACCAAGCAGC ACTCAACCAC GCTAGAGTCC 
TACCTGATCA AACCAGTGCA GAGAGTGCTG AAATACCCCC TGCTGCTCCG CGAGCTGGTG 
TCTCTGACGG ACACGGACAG TGAGGAGCAC TACCACCTCA CAGAGGCGCT TAAGGAAATG 
GAAAAGGTCG CAAGCCATAT AAACGAAATG CAGAAGATCT ACGAAGACTA CGGTGCAGTG 
TTTGACCAGC TGGTGGCAGA GCAAAGTGGC ACAGAGAAAG AGGTCACAGA GATATCTATG 
GGACAGTTCC TCATGCATTC CTCTGTGATC TGGCTCAACC CGTTCCCATC GTTGGGCCGT 
ATGAAAAAGG ATCCCGAGCT GACTGTTTTT GTTTTTAAGA GAGCCGTGAT ACTGCTGTAC 
CGGGAGAACA ACAAGGTGAG GAAGAAGATG GTGACTTCAA GATCAGCCCA CGGAGATCAG 
GATTCATTCA AGTTTCGCTG GCTCATTCCC CTGTCAGCGC TACAGGTCAG ACTGGGCAAC 
ACTGCAGACA CAGACTGCCT CTGGGAGCTG ATTCACACCA AATCACAAAA GGAGGGCAGG 
CCTGAGACGG TGTTCCAGTT GTGCAGCAGC ACACCAGAGT GCAAAGTGAA TATCATCAAG 
GTGATGCGCT CCATCTTGCG GGAGAACCTG CGTCGAAACA TGCAAAGGGT TGAACAGTCT 
GAGAGAACCT GTAAAAAGCG GCTTACTCCC CTTCGGAAAA GCCTGCCAGC TTCGGCTAGG 
ATAGGTTCTT CTCGAGCCTT TTGGCTAGCC AAACAACCTT CAAACGACAG TCCCGTTCCC 
AGCAATGATC CACTGAGGCT GTCTCTAGCA GATTCTGATT CAGCCAGTGC TAGCAGTGGA 
ACCACCAGCA GTGCCAGCGC TCCTCTAGAT CCGCCATCAC AGCAGCAGAG CGGCCTCAGA 
GAATCCAGCA TAATCAGCGA TGAGGACGAA GACGGCAGCG AGCCGGCTGA TTGGACGGAT 
GCTCCCAATG CTATCGAGGT CCAATTTCGC AAGCTTGGTC TGACCGAGGA GACCTCCCAG 
GCAGCTGCTC CACGTGTGCA GCCGGAGAGC GGAGAAGTGG ACACTCCAGA AAGGAAGGCT 
TCTCTAAAGC GAGCTCACTT TGCTGTGGTC AAACGCAAGC CCGCCAGAGA CACGAATAGC 
CTCAGACGCA GTCAGGTGGC CCTGCTGAAC ATGCGTAGAC ACAGCCACTC TTTGGACATC 
CCAGCGGAGA TCGCGGCACA TAGTGTAGAC CTGAACTCGC TACTGGAGAA AGATTTCAGC 
GTGCAGAGCC TGACCTCTGT TGTCAACGAA GACTTCTTCT ATGACAATCC CATGGAGCCA 
AAGAATGTGA CAACTTCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tiam2a ( XM_009293289.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009291564.1 Danio rerio T-cell lymphoma invasion and metastasis 2a (tiam2a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa49965
Clone ID Related Accession (Same CDS sequence)	XM_009293289.2 , XM_009293289.3
Accession Version	XM_009293289.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	T-lymphoma invasion and metastasis-inducing protein 2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293289.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAACT CGGAGAGTCA GTATAGGATC CAGGGCACTA GAAGCAATAG CTTTATTATC 
CCTGACCGAG AGAAACCCTG TTCATTCAGC GTCCACTCCA GCAAAGAAGA CGTTTTATCA 
CCTTTAGGCT TTTGGAATGC TGGTTCAAGA TTTTCTAGAT TGAAATCCGG AAATGAAGGT 
GAAACTTCCT CCACGTCTAA GAGCAGCCGG GCGTTTATAT CCCGCCAGTA TGACTTCATC 
ACCCAGAAAC TAAAGAGTGG TAGTCAGTGG GATGAAGTTC CTGAGGACCT GTTGCCTGGT 
GGCTGCTTGA TGTTGCCTTC GGAAAAGGAC TGCTTGTATC GCAATCACAA GGAGAGGAGA 
AAAACATGTA ATAAATATGC TCTTAATGCA ATGAAGACAC CTGAGAGAAG TCTCAGACTT 
GAGGACATTG AAGACCAGAG CAGCCCTAGG GTTGTCATAA AGAAAGATGG TAGCGTACGT 
GTGGAATTTA ACAATAGTCC CAATAGTGCC CTTATTTTAA ATAATTGCAC TGAACCAGTT 
CAGCTCCTCA AATTTTCCCC CACCATGGAG TCCACTTGTG GTTTATCTGC TGCAGGACCC 
TCATTGGATT CTCATAATGG AGCAGCACAG GCAATGTCCA GCTTGAAAAG CAGCAAAGGT 
AGCTCTCTGA GCTCAGATGG CTCTTTGTAC GACTTGCCTT GGAGGACCAG CGTCGAACTT 
AATGATTCAG ACAATGCTTC TCCTGACCAG CAAAGGCAAA CTGTTTCTAC AAACACAGGC 
TTTTTTGATG AGCAGTTGCC AGCCATTACT ACAACCGACC TCTACAGGGA TCCCAGAATT 
GCTTCCACCT TCCCCACGGT CAAGGATTTG CAGTGTTCAA ATGGCAGCCG TTCACACCAC 
AGATCCTCCT TTGTGTCCGT GATGGAGGGA CTCAGTTCAG AAGGGGTCCC AGAGGAACAG 
CAGTATTCCT CCTTCACCTT GCCTTGCCGG AAATCCAAAG CTTCTCCTGA AAATAATGGA 
AAGAAAGCGT CGATTAGGAG CAGATTTAGA CGCATCAGTG ACTGGACCGG AAGTCTGACC 
AGGAAAAAGA AGTTTCAGGA GCCCAGATAT AACGAGCCGG TCGATTGCTT TGACAGCGGT 
GTGGATGGAC TCACTGCAGA TACCAGTTCC CCCTCTCAGG TCTCCAGCCT TATGGCTACC 
TCCAATGGCA TGTCTCAATC CTGCTCTCTC TCTCGCTCGC TGCACAGCCG CAGTGGCTCT 
GCCGGAGCCC TTAATTTAGG AAATGACATT CTACGGCAAA ACATCTACAA GAATTTTATG 
ATGGAGTTAA AGACTGCCCG GCTTCCAGCA GAAAGAGGAG CTGAAGATTG GTGTGAGAGC 
TCCTCAGAGG AAACAGGAAG CAGTAGTCAA GAAATCCCGG GGTCTCTGGA GCAGCTGGAT 
TTGCTGTTCG AGAAAGAACA AGGGGTAGTT AGGCGAGCTG GGTGGCTGTC CTTCAAGCCC 
TTGCTCACTG TACACAAGGA CCGCAAACTG GAGCTAGTGA GCCGTCGAAA ATGGAAGCAA 
TTCTGGGTCA CTCTCAAGGG TTGCACACTG CTGTTTTATG AAACCTATGG AAAGGCATCT 
TCAGAGCCAG AATCTCCATG CTATGCCTTG TTCGCAGAAG ATGGCGTAGT CCAGGCTGTT 
CCCGAACACC CTAAGAAGGA GAATATCTTC TGCCTTAGTA ACACCTATGG CGATGTCTAC 
CTTTTCCAGG CCTCCAGTCA GACTGACCTA GAGAACTGGG TTACTGCCAT TCATTCAGCA 
AGTGCCTCTT TGTTTGCGAA ACGCTACGGC AAAGAGGACA CACTGCGATT GTTACGTGGG 
CAGATCCGTG GCCTTCTCCA GAAAATCGAC ATGGATGGCA AAATGAAGAA AATGGCCGAG 
CTTCAGCTGT CCGTCGTTGG GGACCCCAAA AACAGAAAAG CTATTGAAAA CCAGATTCAA 
CAATGGGAGC AGAATCTCGA GCGCTTCAAT ATGGACCTTT TCCGCATGCG ATGCTACCTG 
GCCAGTCTGC AAGGAGGCGA GCTCCCCAAC CCAAAGAGCC TCCTAGCCAC GGCCAGCCGC 
CGCACCAAAG CTGCACTAGC CCGATTGGGA ATCTTCTCCG TCTCCTCCTT CCATGCACTG 
ATCTGTTCCA GAGATGAGGC CACGATGCGA AAGCGCTCTC TGTCTCTCTC CCACAGAGGC 
CGCAGCAAGA AAGGGGTCTT TTCATCCCTT AAGGGCCTGG ATACTCTTAC CAAGCGAGGC 
CATGAAAAAA AGCATTCTCT CTCACAGGTC TTTGACAGTG ATGGCGGAGC GCAGCTAAAT 
TCCGTTACAC ATTGTAGTTT TAAGCGGTTA GGCAGTTTGA CGAATGTTTA CTCAATTGTG 
CCACCTGAAG GCAGCCAGTG GGATTGCAGT CGAGAAAGCA CAACATGCAT CTACATGCCA 
AACTGCCAGA CTGTTACTGT TCTCCTGAAA AGTCATCACA CTGTCATGGA TGTGTTGGCT 
GAGACCTGCA TGCTCGTGGG CCTGGACCCC AGCACACACT GTTTGAGGCT GAGAAGATTC 
ACAGGAGACA AGATGGAGAT CTCAACACCA TCAGCTCATG AGCTTCTGCA AGGCTTGCCA 
TATGACGAAT TGGAAGTTAG CCAGCTGCAA GTCTACACTC TGCACATGTC TAGACCCAGT 
CTAACTGGAG ACTTTGGCTT TGCTGTTACA GGCTATGTAG ACAGCCTCAA AAACAGCCAT 
ATCATTGTCA GCGAAGTTCT CCCTGATGGG CTCGCTTTCA GCAAAGGTCT TAGGCCTGGT 
GATGAGATCG TGGTTCTGAA CGGTTGTGAG GTGTCTTCTC TGGACCTGAC TCTCATACAG 
ACTTTATTTA ATGAGCAGAA TCTGCAGCTG ACTGCGAAAC GTCACCTGCC CTCCATTCCA 
CATGATTCAG ACACAGCAAA CGTGCAGTCC CCGTACAGAG ATATCCATCA AAACCGCCAG 
AGAGCCAAGT CCTCAACAGA TGTGTCCTGT GTGACAGAAA CCTTGTCTGT AAATGATGGT 
CTTCAAGATT CTTACCACAA CCATCGCCAA GTTCATCACA GCAAGAGTGT GGACACGGTG 
TGTTCGCTGT ACCACTCCTA CCAGGAAGGC TCTGGAGGTC TGATGGAGAG CCAGGAGGAG 
CAAACGGGGC CTGAACCCGC TATCCTCAGG CCCTGTCCGC GTCATATGTC TGCCACAGAG 
AGACTACGCA AGGTCATCCA GGAATTAGTG GATACAGAGA AATCCTACGT AAAGGACCTC 
AACTGCTTGT TTGAAATCTA CCTGAAGCCC CTGCAAAAGG AGACCTTTCT CACTCAAGAT 
GAGATGGAAA GCCTATTTGG CAGCCTTCCA GAGATGCTGG ACTTCCAGAA AGTTTTCCTG 
CAGACCCTGG AAGAGAAGAT TAGTTCCTCC CCCAACTTCA ACACGTTCGA GACCCCTGTC 
CAATTCAAGA AGCTGCTCTT CTCTCTGGGT GGCTCATTTC TGTATTATGC GGAGCACTTC 
AAACTGTACA GCGGCTTCTG CGCCAACCAT ATTAAAGTGC AGAAGGTTCT GGAACGAGCT 
AAAACAGATC GATCTTTTAA AGAGTTCCTC GATGCTAGGA ACCCGACCAA GCAGCACTCA 
ACCACGCTAG AGTCCTACCT GATCAAACCA GTGCAGAGAG TGCTGAAATA CCCCCTGCTG 
CTCCGCGAGC TGGTGTCTCT GACGGACACG GACAGTGAGG AGCACTACCA CCTCACAGAG 
GCGCTTAAGG AAATGGAAAA GGTCGCAAGC CATATAAACG AAATGCAGAA GATCTACGAA 
GACTACGGTG CAGTGTTTGA CCAGCTGGTG GCAGAGCAAA GTGGCACAGA GAAAGAGGTC 
ACAGAGATAT CTATGGGACA GTTCCTCATG CATTCCTCTG TGATCTGGCT CAACCCGTTC 
CCATCGTTGG GCCGTATGAA AAAGGATCCC GAGCTGACTG TTTTTGTTTT TAAGAGAGCC 
GTGATACTGC TGTACCGGGA GAACAACAAG GTGAGGAAGA AGATGGTGAC TTCAAGATCA 
GCCCACGGAG ATCAGGATTC ATTCAAGTTT CGCTGGCTCA TTCCCCTGTC AGCGCTACAG 
GTCAGACTGG GCAACACTGC AGACACAGAC TGCCTCTGGG AGCTGATTCA CACCAAATCA 
CAAAAGGAGG GCAGGCCTGA GACGGTGTTC CAGTTGTGCA GCAGCACACC AGAGTGCAAA 
GTGAATATCA TCAAGGTGAT GCGCTCCATC TTGCGGGAGA ACCTGCGTCG AAACATGCAA 
AGGGTTGAAC AGTCTGAGAG AACCTGTAAA AAGCGGCTTA CTCCCCTTCG GAAAAGCCTG 
CCAGCTTCGG CTAGGATAGG TTCTTCTCGA GCCTTTTGGC TAGCCAAACA ACCTTCAAAC 
GACAGTCCCG TTCCCAGCAA TGATCCACTG AGGCTGTCTC TAGCAGATTC TGATTCAGCC 
AGTGCTAGCA GTGGAACCAC CAGCAGTGCC AGCGCTCCTC TAGATCCGCC ATCACAGCAG 
CAGAGCGGCC TCAGAGAATC CAGCATAATC AGCGATGAGG ACGAAGACGG CAGCGAGCCG 
GCTGATTGGA CGGATGCTCC CAATGCTATC GAGGTCCAAT TTCGCAAGCT TGGTCTGACC 
GAGGAGACCT CCCAGGCAGC TGCTCCACGT GTGCAGCCGG AGAGCGGAGA AGTGGACACT 
CCAGAAAGGA AGGCTTCTCT AAAGCGAGCT CACTTTGCTG TGGTCAAACG CAAGCCCGCC 
AGAGACACGA ATAGCCTCAG ACGCAGTCAG GTGGCCCTGC TGAACATGCG TAGACACAGC 
CACTCTTTGG ACATCCCAGC GGAGATCGCG GCACATAGTG TAGACCTGAA CTCGCTACTG 
GAGAAAGATT TCAGCGTGCA GAGCCTGACC TCTGTTGTCA ACGAAGACTT CTTCTATGAC 
AATCCCATGG AGCCAAAGAA TGTGACAACT TCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291564.1
CDS	546..5561
Translation	MGNSESQYRIQGTRSNSFIIPDREKPCSFSVHSSKEDVLSPLGFWNAGSRFSRLKSGNEGETSSTSKSSRAFISRQYDFITQKLKSGSQWDEVPEDLLPGGCLMLPSEKDCLYRNHKERRKTCNKYALNAMKTPERSLRLEDIEDQSSPRVVIKKDGSVRVEFNNSPNSALILNNCTEPVQLLKFSPTMESTCGLSAAGPSLDSHNGAAQAMSSLKSSKGSSLSSDGSLYDLPWRTSVELNDSDNASPDQQRQTVSTNTGFFDEQLPAITTTDLYRDPRIASTFPTVKDLQCSNGSRSHHRSSFVSVMEGLSSEGVPEEQQYSSFTLPCRKSKASPENNGKKASIRSRFRRISDWTGSLTRKKKFQEPRYNEPVDCFDSGVDGLTADTSSPSQVSSLMATSNGMSQSCSLSRSLHSRSGSAGALNLGNDILRQNIYKNFMMELKTARLPAERGAEDWCESSSEETGSSSQEIPGSLEQLDLLFEKEQGVVRRAGWLSFKPLLTVHKDRKLELVSRRKWKQFWVTLKGCTLLFYETYGKASSEPESPCYALFAEDGVVQAVPEHPKKENIFCLSNTYGDVYLFQASSQTDLENWVTAIHSASASLFAKRYGKEDTLRLLRGQIRGLLQKIDMDGKMKKMAELQLSVVGDPKNRKAIENQIQQWEQNLERFNMDLFRMRCYLASLQGGELPNPKSLLATASRRTKAALARLGIFSVSSFHALICSRDEATMRKRSLSLSHRGRSKKGVFSSLKGLDTLTKRGHEKKHSLSQVFDSDGGAQLNSVTHCSFKRLGSLTNVYSIVPPEGSQWDCSRESTTCIYMPNCQTVTVLLKSHHTVMDVLAETCMLVGLDPSTHCLRLRRFTGDKMEISTPSAHELLQGLPYDELEVSQLQVYTLHMSRPSLTGDFGFAVTGYVDSLKNSHIIVSEVLPDGLAFSKGLRPGDEIVVLNGCEVSSLDLTLIQTLFNEQNLQLTAKRHLPSIPHDSDTANVQSPYRDIHQNRQRAKSSTDVSCVTETLSVNDGLQDSYHNHRQVHHSKSVDTVCSLYHSYQEGSGGLMESQEEQTGPEPAILRPCPRHMSATERLRKVIQELVDTEKSYVKDLNCLFEIYLKPLQKETFLTQDEMESLFGSLPEMLDFQKVFLQTLEEKISSSPNFNTFETPVQFKKLLFSLGGSFLYYAEHFKLYSGFCANHIKVQKVLERAKTDRSFKEFLDARNPTKQHSTTLESYLIKPVQRVLKYPLLLRELVSLTDTDSEEHYHLTEALKEMEKVASHINEMQKIYEDYGAVFDQLVAEQSGTEKEVTEISMGQFLMHSSVIWLNPFPSLGRMKKDPELTVFVFKRAVILLYRENNKVRKKMVTSRSAHGDQDSFKFRWLIPLSALQVRLGNTADTDCLWELIHTKSQKEGRPETVFQLCSSTPECKVNIIKVMRSILRENLRRNMQRVEQSERTCKKRLTPLRKSLPASARIGSSRAFWLAKQPSNDSPVPSNDPLRLSLADSDSASASSGTTSSASAPLDPPSQQQSGLRESSIISDEDEDGSEPADWTDAPNAIEVQFRKLGLTEETSQAAAPRVQPESGEVDTPERKASLKRAHFAVVKRKPARDTNSLRRSQVALLNMRRHSHSLDIPAEIAAHSVDLNSLLEKDFSVQSLTSVVNEDFFYDNPMEPKNVTTS

Target ORF information:

RefSeq Version	XM_009293289.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio T-cell lymphoma invasion and metastasis 2a (tiam2a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293289.2

ORF Insert Sequence:

ATGGGAAACT CGGAGAGTCA GTATAGGATC CAGGGCACTA GAAGCAATAG CTTTATTATC 
CCTGACCGAG AGAAACCCTG TTCATTCAGC GTCCACTCCA GCAAAGAAGA CGTTTTATCA 
CCTTTAGGCT TTTGGAATGC TGGTTCAAGA TTTTCTAGAT TGAAATCCGG AAATGAAGGT 
GAAACTTCCT CCACGTCTAA GAGCAGCCGG GCGTTTATAT CCCGCCAGTA TGACTTCATC 
ACCCAGAAAC TAAAGAGTGG TAGTCAGTGG GATGAAGTTC CTGAGGACCT GTTGCCTGGT 
GGCTGCTTGA TGTTGCCTTC GGAAAAGGAC TGCTTGTATC GCAATCACAA GGAGAGGAGA 
AAAACATGTA ATAAATATGC TCTTAATGCA ATGAAGACAC CTGAGAGAAG TCTCAGACTT 
GAGGACATTG AAGACCAGAG CAGCCCTAGG GTTGTCATAA AGAAAGATGG TAGCGTACGT 
GTGGAATTTA ACAATAGTCC CAATAGTGCC CTTATTTTAA ATAATTGCAC TGAACCAGTT 
CAGCTCCTCA AATTTTCCCC CACCATGGAG TCCACTTGTG GTTTATCTGC TGCAGGACCC 
TCATTGGATT CTCATAATGG AGCAGCACAG GCAATGTCCA GCTTGAAAAG CAGCAAAGGT 
AGCTCTCTGA GCTCAGATGG CTCTTTGTAC GACTTGCCTT GGAGGACCAG CGTCGAACTT 
AATGATTCAG ACAATGCTTC TCCTGACCAG CAAAGGCAAA CTGTTTCTAC AAACACAGGC 
TTTTTTGATG AGCAGTTGCC AGCCATTACT ACAACCGACC TCTACAGGGA TCCCAGAATT 
GCTTCCACCT TCCCCACGGT CAAGGATTTG CAGTGTTCAA ATGGCAGCCG TTCACACCAC 
AGATCCTCCT TTGTGTCCGT GATGGAGGGA CTCAGTTCAG AAGGGGTCCC AGAGGAACAG 
CAGTATTCCT CCTTCACCTT GCCTTGCCGG AAATCCAAAG CTTCTCCTGA AAATAATGGA 
AAGAAAGCGT CGATTAGGAG CAGATTTAGA CGCATCAGTG ACTGGACCGG AAGTCTGACC 
AGGAAAAAGA AGTTTCAGGA GCCCAGATAT AACGAGCCGG TCGATTGCTT TGACAGCGGT 
GTGGATGGAC TCACTGCAGA TACCAGTTCC CCCTCTCAGG TCTCCAGCCT TATGGCTACC 
TCCAATGGCA TGTCTCAATC CTGCTCTCTC TCTCGCTCGC TGCACAGCCG CAGTGGCTCT 
GCCGGAGCCC TTAATTTAGG AAATGACATT CTACGGCAAA ACATCTACAA GAATTTTATG 
ATGGAGTTAA AGACTGCCCG GCTTCCAGCA GAAAGAGGAG CTGAAGATTG GTGTGAGAGC 
TCCTCAGAGG AAACAGGAAG CAGTAGTCAA GAAATCCCGG GGTCTCTGGA GCAGCTGGAT 
TTGCTGTTCG AGAAAGAACA AGGGGTAGTT AGGCGAGCTG GGTGGCTGTC CTTCAAGCCC 
TTGCTCACTG TACACAAGGA CCGCAAACTG GAGCTAGTGA GCCGTCGAAA ATGGAAGCAA 
TTCTGGGTCA CTCTCAAGGG TTGCACACTG CTGTTTTATG AAACCTATGG AAAGGCATCT 
TCAGAGCCAG AATCTCCATG CTATGCCTTG TTCGCAGAAG ATGGCGTAGT CCAGGCTGTT 
CCCGAACACC CTAAGAAGGA GAATATCTTC TGCCTTAGTA ACACCTATGG CGATGTCTAC 
CTTTTCCAGG CCTCCAGTCA GACTGACCTA GAGAACTGGG TTACTGCCAT TCATTCAGCA 
AGTGCCTCTT TGTTTGCGAA ACGCTACGGC AAAGAGGACA CACTGCGATT GTTACGTGGG 
CAGATCCGTG GCCTTCTCCA GAAAATCGAC ATGGATGGCA AAATGAAGAA AATGGCCGAG 
CTTCAGCTGT CCGTCGTTGG GGACCCCAAA AACAGAAAAG CTATTGAAAA CCAGATTCAA 
CAATGGGAGC AGAATCTCGA GCGCTTCAAT ATGGACCTTT TCCGCATGCG ATGCTACCTG 
GCCAGTCTGC AAGGAGGCGA GCTCCCCAAC CCAAAGAGCC TCCTAGCCAC GGCCAGCCGC 
CGCACCAAAG CTGCACTAGC CCGATTGGGA ATCTTCTCCG TCTCCTCCTT CCATGCACTG 
ATCTGTTCCA GAGATGAGGC CACGATGCGA AAGCGCTCTC TGTCTCTCTC CCACAGAGGC 
CGCAGCAAGA AAGGGGTCTT TTCATCCCTT AAGGGCCTGG ATACTCTTAC CAAGCGAGGC 
CATGAAAAAA AGCATTCTCT CTCACAGGTC TTTGACAGTG ATGGCGGAGC GCAGCTAAAT 
TCCGTTACAC ATTGTAGTTT TAAGCGGTTA GGCAGTTTGA CGAATGTTTA CTCAATTGTG 
CCACCTGAAG GCAGCCAGTG GGATTGCAGT CGAGAAAGCA CAACATGCAT CTACATGCCA 
AACTGCCAGA CTGTTACTGT TCTCCTGAAA AGTCATCACA CTGTCATGGA TGTGTTGGCT 
GAGACCTGCA TGCTCGTGGG CCTGGACCCC AGCACACACT GTTTGAGGCT GAGAAGATTC 
ACAGGAGACA AGATGGAGAT CTCAACACCA TCAGCTCATG AGCTTCTGCA AGGCTTGCCA 
TATGACGAAT TGGAAGTTAG CCAGCTGCAA GTCTACACTC TGCACATGTC TAGACCCAGT 
CTAACTGGAG ACTTTGGCTT TGCTGTTACA GGCTATGTAG ACAGCCTCAA AAACAGCCAT 
ATCATTGTCA GCGAAGTTCT CCCTGATGGG CTCGCTTTCA GCAAAGGTCT TAGGCCTGGT 
GATGAGATCG TGGTTCTGAA CGGTTGTGAG GTGTCTTCTC TGGACCTGAC TCTCATACAG 
ACTTTATTTA ATGAGCAGAA TCTGCAGCTG ACTGCGAAAC GTCACCTGCC CTCCATTCCA 
CATGATTCAG ACACAGCAAA CGTGCAGTCC CCGTACAGAG ATATCCATCA AAACCGCCAG 
AGAGCCAAGT CCTCAACAGA TGTGTCCTGT GTGACAGAAA CCTTGTCTGT AAATGATGGT 
CTTCAAGATT CTTACCACAA CCATCGCCAA GTTCATCACA GCAAGAGTGT GGACACGGTG 
TGTTCGCTGT ACCACTCCTA CCAGGAAGGC TCTGGAGGTC TGATGGAGAG CCAGGAGGAG 
CAAACGGGGC CTGAACCCGC TATCCTCAGG CCCTGTCCGC GTCATATGTC TGCCACAGAG 
AGACTACGCA AGGTCATCCA GGAATTAGTG GATACAGAGA AATCCTACGT AAAGGACCTC 
AACTGCTTGT TTGAAATCTA CCTGAAGCCC CTGCAAAAGG AGACCTTTCT CACTCAAGAT 
GAGATGGAAA GCCTATTTGG CAGCCTTCCA GAGATGCTGG ACTTCCAGAA AGTTTTCCTG 
CAGACCCTGG AAGAGAAGAT TAGTTCCTCC CCCAACTTCA ACACGTTCGA GACCCCTGTC 
CAATTCAAGA AGCTGCTCTT CTCTCTGGGT GGCTCATTTC TGTATTATGC GGAGCACTTC 
AAACTGTACA GCGGCTTCTG CGCCAACCAT ATTAAAGTGC AGAAGGTTCT GGAACGAGCT 
AAAACAGATC GATCTTTTAA AGAGTTCCTC GATGCTAGGA ACCCGACCAA GCAGCACTCA 
ACCACGCTAG AGTCCTACCT GATCAAACCA GTGCAGAGAG TGCTGAAATA CCCCCTGCTG 
CTCCGCGAGC TGGTGTCTCT GACGGACACG GACAGTGAGG AGCACTACCA CCTCACAGAG 
GCGCTTAAGG AAATGGAAAA GGTCGCAAGC CATATAAACG AAATGCAGAA GATCTACGAA 
GACTACGGTG CAGTGTTTGA CCAGCTGGTG GCAGAGCAAA GTGGCACAGA GAAAGAGGTC 
ACAGAGATAT CTATGGGACA GTTCCTCATG CATTCCTCTG TGATCTGGCT CAACCCGTTC 
CCATCGTTGG GCCGTATGAA AAAGGATCCC GAGCTGACTG TTTTTGTTTT TAAGAGAGCC 
GTGATACTGC TGTACCGGGA GAACAACAAG GTGAGGAAGA AGATGGTGAC TTCAAGATCA 
GCCCACGGAG ATCAGGATTC ATTCAAGTTT CGCTGGCTCA TTCCCCTGTC AGCGCTACAG 
GTCAGACTGG GCAACACTGC AGACACAGAC TGCCTCTGGG AGCTGATTCA CACCAAATCA 
CAAAAGGAGG GCAGGCCTGA GACGGTGTTC CAGTTGTGCA GCAGCACACC AGAGTGCAAA 
GTGAATATCA TCAAGGTGAT GCGCTCCATC TTGCGGGAGA ACCTGCGTCG AAACATGCAA 
AGGGTTGAAC AGTCTGAGAG AACCTGTAAA AAGCGGCTTA CTCCCCTTCG GAAAAGCCTG 
CCAGCTTCGG CTAGGATAGG TTCTTCTCGA GCCTTTTGGC TAGCCAAACA ACCTTCAAAC 
GACAGTCCCG TTCCCAGCAA TGATCCACTG AGGCTGTCTC TAGCAGATTC TGATTCAGCC 
AGTGCTAGCA GTGGAACCAC CAGCAGTGCC AGCGCTCCTC TAGATCCGCC ATCACAGCAG 
CAGAGCGGCC TCAGAGAATC CAGCATAATC AGCGATGAGG ACGAAGACGG CAGCGAGCCG 
GCTGATTGGA CGGATGCTCC CAATGCTATC GAGGTCCAAT TTCGCAAGCT TGGTCTGACC 
GAGGAGACCT CCCAGGCAGC TGCTCCACGT GTGCAGCCGG AGAGCGGAGA AGTGGACACT 
CCAGAAAGGA AGGCTTCTCT AAAGCGAGCT CACTTTGCTG TGGTCAAACG CAAGCCCGCC 
AGAGACACGA ATAGCCTCAG ACGCAGTCAG GTGGCCCTGC TGAACATGCG TAGACACAGC 
CACTCTTTGG ACATCCCAGC GGAGATCGCG GCACATAGTG TAGACCTGAA CTCGCTACTG 
GAGAAAGATT TCAGCGTGCA GAGCCTGACC TCTGTTGTCA ACGAAGACTT CTTCTATGAC 
AATCCCATGG AGCCAAAGAA TGTGACAACT TCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tiam2a ( XM_009293289.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009291564.1 Danio rerio T-cell lymphoma invasion and metastasis 2a (tiam2a), transcript variant X3, mRNA.

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CloneID	ODa49965
Clone ID Related Accession (Same CDS sequence)	XM_009293289.2 , XM_009293289.3
Accession Version	XM_009293289.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	T-lymphoma invasion and metastasis-inducing protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293289.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAACT CGGAGAGTCA GTATAGGATC CAGGGCACTA GAAGCAATAG CTTTATTATC 
CCTGACCGAG AGAAACCCTG TTCATTCAGC GTCCACTCCA GCAAAGAAGA CGTTTTATCA 
CCTTTAGGCT TTTGGAATGC TGGTTCAAGA TTTTCTAGAT TGAAATCCGG AAATGAAGGT 
GAAACTTCCT CCACGTCTAA GAGCAGCCGG GCGTTTATAT CCCGCCAGTA TGACTTCATC 
ACCCAGAAAC TAAAGAGTGG TAGTCAGTGG GATGAAGTTC CTGAGGACCT GTTGCCTGGT 
GGCTGCTTGA TGTTGCCTTC GGAAAAGGAC TGCTTGTATC GCAATCACAA GGAGAGGAGA 
AAAACATGTA ATAAATATGC TCTTAATGCA ATGAAGACAC CTGAGAGAAG TCTCAGACTT 
GAGGACATTG AAGACCAGAG CAGCCCTAGG GTTGTCATAA AGAAAGATGG TAGCGTACGT 
GTGGAATTTA ACAATAGTCC CAATAGTGCC CTTATTTTAA ATAATTGCAC TGAACCAGTT 
CAGCTCCTCA AATTTTCCCC CACCATGGAG TCCACTTGTG GTTTATCTGC TGCAGGACCC 
TCATTGGATT CTCATAATGG AGCAGCACAG GCAATGTCCA GCTTGAAAAG CAGCAAAGGT 
AGCTCTCTGA GCTCAGATGG CTCTTTGTAC GACTTGCCTT GGAGGACCAG CGTCGAACTT 
AATGATTCAG ACAATGCTTC TCCTGACCAG CAAAGGCAAA CTGTTTCTAC AAACACAGGC 
TTTTTTGATG AGCAGTTGCC AGCCATTACT ACAACCGACC TCTACAGGGA TCCCAGAATT 
GCTTCCACCT TCCCCACGGT CAAGGATTTG CAGTGTTCAA ATGGCAGCCG TTCACACCAC 
AGATCCTCCT TTGTGTCCGT GATGGAGGGA CTCAGTTCAG AAGGGGTCCC AGAGGAACAG 
CAGTATTCCT CCTTCACCTT GCCTTGCCGG AAATCCAAAG CTTCTCCTGA AAATAATGGA 
AAGAAAGCGT CGATTAGGAG CAGATTTAGA CGCATCAGTG ACTGGACCGG AAGTCTGACC 
AGGAAAAAGA AGTTTCAGGA GCCCAGATAT AACGAGCCGG TCGATTGCTT TGACAGCGGT 
GTGGATGGAC TCACTGCAGA TACCAGTTCC CCCTCTCAGG TCTCCAGCCT TATGGCTACC 
TCCAATGGCA TGTCTCAATC CTGCTCTCTC TCTCGCTCGC TGCACAGCCG CAGTGGCTCT 
GCCGGAGCCC TTAATTTAGG AAATGACATT CTACGGCAAA ACATCTACAA GAATTTTATG 
ATGGAGTTAA AGACTGCCCG GCTTCCAGCA GAAAGAGGAG CTGAAGATTG GTGTGAGAGC 
TCCTCAGAGG AAACAGGAAG CAGTAGTCAA GAAATCCCGG GGTCTCTGGA GCAGCTGGAT 
TTGCTGTTCG AGAAAGAACA AGGGGTAGTT AGGCGAGCTG GGTGGCTGTC CTTCAAGCCC 
TTGCTCACTG TACACAAGGA CCGCAAACTG GAGCTAGTGA GCCGTCGAAA ATGGAAGCAA 
TTCTGGGTCA CTCTCAAGGG TTGCACACTG CTGTTTTATG AAACCTATGG AAAGGCATCT 
TCAGAGCCAG AATCTCCATG CTATGCCTTG TTCGCAGAAG ATGGCGTAGT CCAGGCTGTT 
CCCGAACACC CTAAGAAGGA GAATATCTTC TGCCTTAGTA ACACCTATGG CGATGTCTAC 
CTTTTCCAGG CCTCCAGTCA GACTGACCTA GAGAACTGGG TTACTGCCAT TCATTCAGCA 
AGTGCCTCTT TGTTTGCGAA ACGCTACGGC AAAGAGGACA CACTGCGATT GTTACGTGGG 
CAGATCCGTG GCCTTCTCCA GAAAATCGAC ATGGATGGCA AAATGAAGAA AATGGCCGAG 
CTTCAGCTGT CCGTCGTTGG GGACCCCAAA AACAGAAAAG CTATTGAAAA CCAGATTCAA 
CAATGGGAGC AGAATCTCGA GCGCTTCAAT ATGGACCTTT TCCGCATGCG ATGCTACCTG 
GCCAGTCTGC AAGGAGGCGA GCTCCCCAAC CCAAAGAGCC TCCTAGCCAC GGCCAGCCGC 
CGCACCAAAG CTGCACTAGC CCGATTGGGA ATCTTCTCCG TCTCCTCCTT CCATGCACTG 
ATCTGTTCCA GAGATGAGGC CACGATGCGA AAGCGCTCTC TGTCTCTCTC CCACAGAGGC 
CGCAGCAAGA AAGGGGTCTT TTCATCCCTT AAGGGCCTGG ATACTCTTAC CAAGCGAGGC 
CATGAAAAAA AGCATTCTCT CTCACAGGTC TTTGACAGTG ATGGCGGAGC GCAGCTAAAT 
TCCGTTACAC ATTGTAGTTT TAAGCGGTTA GGCAGTTTGA CGAATGTTTA CTCAATTGTG 
CCACCTGAAG GCAGCCAGTG GGATTGCAGT CGAGAAAGCA CAACATGCAT CTACATGCCA 
AACTGCCAGA CTGTTACTGT TCTCCTGAAA AGTCATCACA CTGTCATGGA TGTGTTGGCT 
GAGACCTGCA TGCTCGTGGG CCTGGACCCC AGCACACACT GTTTGAGGCT GAGAAGATTC 
ACAGGAGACA AGATGGAGAT CTCAACACCA TCAGCTCATG AGCTTCTGCA AGGCTTGCCA 
TATGACGAAT TGGAAGTTAG CCAGCTGCAA GTCTACACTC TGCACATGTC TAGACCCAGT 
CTAACTGGAG ACTTTGGCTT TGCTGTTACA GGCTATGTAG ACAGCCTCAA AAACAGCCAT 
ATCATTGTCA GCGAAGTTCT CCCTGATGGG CTCGCTTTCA GCAAAGGTCT TAGGCCTGGT 
GATGAGATCG TGGTTCTGAA CGGTTGTGAG GTGTCTTCTC TGGACCTGAC TCTCATACAG 
ACTTTATTTA ATGAGCAGAA TCTGCAGCTG ACTGCGAAAC GTCACCTGCC CTCCATTCCA 
CATGATTCAG ACACAGCAAA CGTGCAGTCC CCGTACAGAG ATATCCATCA AAACCGCCAG 
AGAGCCAAGT CCTCAACAGA TGTGTCCTGT GTGACAGAAA CCTTGTCTGT AAATGATGGT 
CTTCAAGATT CTTACCACAA CCATCGCCAA GTTCATCACA GCAAGAGTGT GGACACGGTG 
TGTTCGCTGT ACCACTCCTA CCAGGAAGGC TCTGGAGGTC TGATGGAGAG CCAGGAGGAG 
CAAACGGGGC CTGAACCCGC TATCCTCAGG CCCTGTCCGC GTCATATGTC TGCCACAGAG 
AGACTACGCA AGGTCATCCA GGAATTAGTG GATACAGAGA AATCCTACGT AAAGGACCTC 
AACTGCTTGT TTGAAATCTA CCTGAAGCCC CTGCAAAAGG AGACCTTTCT CACTCAAGAT 
GAGATGGAAA GCCTATTTGG CAGCCTTCCA GAGATGCTGG ACTTCCAGAA AGTTTTCCTG 
CAGACCCTGG AAGAGAAGAT TAGTTCCTCC CCCAACTTCA ACACGTTCGA GACCCCTGTC 
CAATTCAAGA AGCTGCTCTT CTCTCTGGGT GGCTCATTTC TGTATTATGC GGAGCACTTC 
AAACTGTACA GCGGCTTCTG CGCCAACCAT ATTAAAGTGC AGAAGGTTCT GGAACGAGCT 
AAAACAGATC GATCTTTTAA AGAGTTCCTC GATGCTAGGA ACCCGACCAA GCAGCACTCA 
ACCACGCTAG AGTCCTACCT GATCAAACCA GTGCAGAGAG TGCTGAAATA CCCCCTGCTG 
CTCCGCGAGC TGGTGTCTCT GACGGACACG GACAGTGAGG AGCACTACCA CCTCACAGAG 
GCGCTTAAGG AAATGGAAAA GGTCGCAAGC CATATAAACG AAATGCAGAA GATCTACGAA 
GACTACGGTG CAGTGTTTGA CCAGCTGGTG GCAGAGCAAA GTGGCACAGA GAAAGAGGTC 
ACAGAGATAT CTATGGGACA GTTCCTCATG CATTCCTCTG TGATCTGGCT CAACCCGTTC 
CCATCGTTGG GCCGTATGAA AAAGGATCCC GAGCTGACTG TTTTTGTTTT TAAGAGAGCC 
GTGATACTGC TGTACCGGGA GAACAACAAG GTGAGGAAGA AGATGGTGAC TTCAAGATCA 
GCCCACGGAG ATCAGGATTC ATTCAAGTTT CGCTGGCTCA TTCCCCTGTC AGCGCTACAG 
GTCAGACTGG GCAACACTGC AGACACAGAC TGCCTCTGGG AGCTGATTCA CACCAAATCA 
CAAAAGGAGG GCAGGCCTGA GACGGTGTTC CAGTTGTGCA GCAGCACACC AGAGTGCAAA 
GTGAATATCA TCAAGGTGAT GCGCTCCATC TTGCGGGAGA ACCTGCGTCG AAACATGCAA 
AGGGTTGAAC AGTCTGAGAG AACCTGTAAA AAGCGGCTTA CTCCCCTTCG GAAAAGCCTG 
CCAGCTTCGG CTAGGATAGG TTCTTCTCGA GCCTTTTGGC TAGCCAAACA ACCTTCAAAC 
GACAGTCCCG TTCCCAGCAA TGATCCACTG AGGCTGTCTC TAGCAGATTC TGATTCAGCC 
AGTGCTAGCA GTGGAACCAC CAGCAGTGCC AGCGCTCCTC TAGATCCGCC ATCACAGCAG 
CAGAGCGGCC TCAGAGAATC CAGCATAATC AGCGATGAGG ACGAAGACGG CAGCGAGCCG 
GCTGATTGGA CGGATGCTCC CAATGCTATC GAGGTCCAAT TTCGCAAGCT TGGTCTGACC 
GAGGAGACCT CCCAGGCAGC TGCTCCACGT GTGCAGCCGG AGAGCGGAGA AGTGGACACT 
CCAGAAAGGA AGGCTTCTCT AAAGCGAGCT CACTTTGCTG TGGTCAAACG CAAGCCCGCC 
AGAGACACGA ATAGCCTCAG ACGCAGTCAG GTGGCCCTGC TGAACATGCG TAGACACAGC 
CACTCTTTGG ACATCCCAGC GGAGATCGCG GCACATAGTG TAGACCTGAA CTCGCTACTG 
GAGAAAGATT TCAGCGTGCA GAGCCTGACC TCTGTTGTCA ACGAAGACTT CTTCTATGAC 
AATCCCATGG AGCCAAAGAA TGTGACAACT TCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291564.1
CDS	489..5504
Translation	MGNSESQYRIQGTRSNSFIIPDREKPCSFSVHSSKEDVLSPLGFWNAGSRFSRLKSGNEGETSSTSKSSRAFISRQYDFITQKLKSGSQWDEVPEDLLPGGCLMLPSEKDCLYRNHKERRKTCNKYALNAMKTPERSLRLEDIEDQSSPRVVIKKDGSVRVEFNNSPNSALILNNCTEPVQLLKFSPTMESTCGLSAAGPSLDSHNGAAQAMSSLKSSKGSSLSSDGSLYDLPWRTSVELNDSDNASPDQQRQTVSTNTGFFDEQLPAITTTDLYRDPRIASTFPTVKDLQCSNGSRSHHRSSFVSVMEGLSSEGVPEEQQYSSFTLPCRKSKASPENNGKKASIRSRFRRISDWTGSLTRKKKFQEPRYNEPVDCFDSGVDGLTADTSSPSQVSSLMATSNGMSQSCSLSRSLHSRSGSAGALNLGNDILRQNIYKNFMMELKTARLPAERGAEDWCESSSEETGSSSQEIPGSLEQLDLLFEKEQGVVRRAGWLSFKPLLTVHKDRKLELVSRRKWKQFWVTLKGCTLLFYETYGKASSEPESPCYALFAEDGVVQAVPEHPKKENIFCLSNTYGDVYLFQASSQTDLENWVTAIHSASASLFAKRYGKEDTLRLLRGQIRGLLQKIDMDGKMKKMAELQLSVVGDPKNRKAIENQIQQWEQNLERFNMDLFRMRCYLASLQGGELPNPKSLLATASRRTKAALARLGIFSVSSFHALICSRDEATMRKRSLSLSHRGRSKKGVFSSLKGLDTLTKRGHEKKHSLSQVFDSDGGAQLNSVTHCSFKRLGSLTNVYSIVPPEGSQWDCSRESTTCIYMPNCQTVTVLLKSHHTVMDVLAETCMLVGLDPSTHCLRLRRFTGDKMEISTPSAHELLQGLPYDELEVSQLQVYTLHMSRPSLTGDFGFAVTGYVDSLKNSHIIVSEVLPDGLAFSKGLRPGDEIVVLNGCEVSSLDLTLIQTLFNEQNLQLTAKRHLPSIPHDSDTANVQSPYRDIHQNRQRAKSSTDVSCVTETLSVNDGLQDSYHNHRQVHHSKSVDTVCSLYHSYQEGSGGLMESQEEQTGPEPAILRPCPRHMSATERLRKVIQELVDTEKSYVKDLNCLFEIYLKPLQKETFLTQDEMESLFGSLPEMLDFQKVFLQTLEEKISSSPNFNTFETPVQFKKLLFSLGGSFLYYAEHFKLYSGFCANHIKVQKVLERAKTDRSFKEFLDARNPTKQHSTTLESYLIKPVQRVLKYPLLLRELVSLTDTDSEEHYHLTEALKEMEKVASHINEMQKIYEDYGAVFDQLVAEQSGTEKEVTEISMGQFLMHSSVIWLNPFPSLGRMKKDPELTVFVFKRAVILLYRENNKVRKKMVTSRSAHGDQDSFKFRWLIPLSALQVRLGNTADTDCLWELIHTKSQKEGRPETVFQLCSSTPECKVNIIKVMRSILRENLRRNMQRVEQSERTCKKRLTPLRKSLPASARIGSSRAFWLAKQPSNDSPVPSNDPLRLSLADSDSASASSGTTSSASAPLDPPSQQQSGLRESSIISDEDEDGSEPADWTDAPNAIEVQFRKLGLTEETSQAAAPRVQPESGEVDTPERKASLKRAHFAVVKRKPARDTNSLRRSQVALLNMRRHSHSLDIPAEIAAHSVDLNSLLEKDFSVQSLTSVVNEDFFYDNPMEPKNVTTS

Target ORF information:

RefSeq Version	XM_009293289.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio T-cell lymphoma invasion and metastasis 2a (tiam2a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293289.3

ORF Insert Sequence:

ATGGGAAACT CGGAGAGTCA GTATAGGATC CAGGGCACTA GAAGCAATAG CTTTATTATC 
CCTGACCGAG AGAAACCCTG TTCATTCAGC GTCCACTCCA GCAAAGAAGA CGTTTTATCA 
CCTTTAGGCT TTTGGAATGC TGGTTCAAGA TTTTCTAGAT TGAAATCCGG AAATGAAGGT 
GAAACTTCCT CCACGTCTAA GAGCAGCCGG GCGTTTATAT CCCGCCAGTA TGACTTCATC 
ACCCAGAAAC TAAAGAGTGG TAGTCAGTGG GATGAAGTTC CTGAGGACCT GTTGCCTGGT 
GGCTGCTTGA TGTTGCCTTC GGAAAAGGAC TGCTTGTATC GCAATCACAA GGAGAGGAGA 
AAAACATGTA ATAAATATGC TCTTAATGCA ATGAAGACAC CTGAGAGAAG TCTCAGACTT 
GAGGACATTG AAGACCAGAG CAGCCCTAGG GTTGTCATAA AGAAAGATGG TAGCGTACGT 
GTGGAATTTA ACAATAGTCC CAATAGTGCC CTTATTTTAA ATAATTGCAC TGAACCAGTT 
CAGCTCCTCA AATTTTCCCC CACCATGGAG TCCACTTGTG GTTTATCTGC TGCAGGACCC 
TCATTGGATT CTCATAATGG AGCAGCACAG GCAATGTCCA GCTTGAAAAG CAGCAAAGGT 
AGCTCTCTGA GCTCAGATGG CTCTTTGTAC GACTTGCCTT GGAGGACCAG CGTCGAACTT 
AATGATTCAG ACAATGCTTC TCCTGACCAG CAAAGGCAAA CTGTTTCTAC AAACACAGGC 
TTTTTTGATG AGCAGTTGCC AGCCATTACT ACAACCGACC TCTACAGGGA TCCCAGAATT 
GCTTCCACCT TCCCCACGGT CAAGGATTTG CAGTGTTCAA ATGGCAGCCG TTCACACCAC 
AGATCCTCCT TTGTGTCCGT GATGGAGGGA CTCAGTTCAG AAGGGGTCCC AGAGGAACAG 
CAGTATTCCT CCTTCACCTT GCCTTGCCGG AAATCCAAAG CTTCTCCTGA AAATAATGGA 
AAGAAAGCGT CGATTAGGAG CAGATTTAGA CGCATCAGTG ACTGGACCGG AAGTCTGACC 
AGGAAAAAGA AGTTTCAGGA GCCCAGATAT AACGAGCCGG TCGATTGCTT TGACAGCGGT 
GTGGATGGAC TCACTGCAGA TACCAGTTCC CCCTCTCAGG TCTCCAGCCT TATGGCTACC 
TCCAATGGCA TGTCTCAATC CTGCTCTCTC TCTCGCTCGC TGCACAGCCG CAGTGGCTCT 
GCCGGAGCCC TTAATTTAGG AAATGACATT CTACGGCAAA ACATCTACAA GAATTTTATG 
ATGGAGTTAA AGACTGCCCG GCTTCCAGCA GAAAGAGGAG CTGAAGATTG GTGTGAGAGC 
TCCTCAGAGG AAACAGGAAG CAGTAGTCAA GAAATCCCGG GGTCTCTGGA GCAGCTGGAT 
TTGCTGTTCG AGAAAGAACA AGGGGTAGTT AGGCGAGCTG GGTGGCTGTC CTTCAAGCCC 
TTGCTCACTG TACACAAGGA CCGCAAACTG GAGCTAGTGA GCCGTCGAAA ATGGAAGCAA 
TTCTGGGTCA CTCTCAAGGG TTGCACACTG CTGTTTTATG AAACCTATGG AAAGGCATCT 
TCAGAGCCAG AATCTCCATG CTATGCCTTG TTCGCAGAAG ATGGCGTAGT CCAGGCTGTT 
CCCGAACACC CTAAGAAGGA GAATATCTTC TGCCTTAGTA ACACCTATGG CGATGTCTAC 
CTTTTCCAGG CCTCCAGTCA GACTGACCTA GAGAACTGGG TTACTGCCAT TCATTCAGCA 
AGTGCCTCTT TGTTTGCGAA ACGCTACGGC AAAGAGGACA CACTGCGATT GTTACGTGGG 
CAGATCCGTG GCCTTCTCCA GAAAATCGAC ATGGATGGCA AAATGAAGAA AATGGCCGAG 
CTTCAGCTGT CCGTCGTTGG GGACCCCAAA AACAGAAAAG CTATTGAAAA CCAGATTCAA 
CAATGGGAGC AGAATCTCGA GCGCTTCAAT ATGGACCTTT TCCGCATGCG ATGCTACCTG 
GCCAGTCTGC AAGGAGGCGA GCTCCCCAAC CCAAAGAGCC TCCTAGCCAC GGCCAGCCGC 
CGCACCAAAG CTGCACTAGC CCGATTGGGA ATCTTCTCCG TCTCCTCCTT CCATGCACTG 
ATCTGTTCCA GAGATGAGGC CACGATGCGA AAGCGCTCTC TGTCTCTCTC CCACAGAGGC 
CGCAGCAAGA AAGGGGTCTT TTCATCCCTT AAGGGCCTGG ATACTCTTAC CAAGCGAGGC 
CATGAAAAAA AGCATTCTCT CTCACAGGTC TTTGACAGTG ATGGCGGAGC GCAGCTAAAT 
TCCGTTACAC ATTGTAGTTT TAAGCGGTTA GGCAGTTTGA CGAATGTTTA CTCAATTGTG 
CCACCTGAAG GCAGCCAGTG GGATTGCAGT CGAGAAAGCA CAACATGCAT CTACATGCCA 
AACTGCCAGA CTGTTACTGT TCTCCTGAAA AGTCATCACA CTGTCATGGA TGTGTTGGCT 
GAGACCTGCA TGCTCGTGGG CCTGGACCCC AGCACACACT GTTTGAGGCT GAGAAGATTC 
ACAGGAGACA AGATGGAGAT CTCAACACCA TCAGCTCATG AGCTTCTGCA AGGCTTGCCA 
TATGACGAAT TGGAAGTTAG CCAGCTGCAA GTCTACACTC TGCACATGTC TAGACCCAGT 
CTAACTGGAG ACTTTGGCTT TGCTGTTACA GGCTATGTAG ACAGCCTCAA AAACAGCCAT 
ATCATTGTCA GCGAAGTTCT CCCTGATGGG CTCGCTTTCA GCAAAGGTCT TAGGCCTGGT 
GATGAGATCG TGGTTCTGAA CGGTTGTGAG GTGTCTTCTC TGGACCTGAC TCTCATACAG 
ACTTTATTTA ATGAGCAGAA TCTGCAGCTG ACTGCGAAAC GTCACCTGCC CTCCATTCCA 
CATGATTCAG ACACAGCAAA CGTGCAGTCC CCGTACAGAG ATATCCATCA AAACCGCCAG 
AGAGCCAAGT CCTCAACAGA TGTGTCCTGT GTGACAGAAA CCTTGTCTGT AAATGATGGT 
CTTCAAGATT CTTACCACAA CCATCGCCAA GTTCATCACA GCAAGAGTGT GGACACGGTG 
TGTTCGCTGT ACCACTCCTA CCAGGAAGGC TCTGGAGGTC TGATGGAGAG CCAGGAGGAG 
CAAACGGGGC CTGAACCCGC TATCCTCAGG CCCTGTCCGC GTCATATGTC TGCCACAGAG 
AGACTACGCA AGGTCATCCA GGAATTAGTG GATACAGAGA AATCCTACGT AAAGGACCTC 
AACTGCTTGT TTGAAATCTA CCTGAAGCCC CTGCAAAAGG AGACCTTTCT CACTCAAGAT 
GAGATGGAAA GCCTATTTGG CAGCCTTCCA GAGATGCTGG ACTTCCAGAA AGTTTTCCTG 
CAGACCCTGG AAGAGAAGAT TAGTTCCTCC CCCAACTTCA ACACGTTCGA GACCCCTGTC 
CAATTCAAGA AGCTGCTCTT CTCTCTGGGT GGCTCATTTC TGTATTATGC GGAGCACTTC 
AAACTGTACA GCGGCTTCTG CGCCAACCAT ATTAAAGTGC AGAAGGTTCT GGAACGAGCT 
AAAACAGATC GATCTTTTAA AGAGTTCCTC GATGCTAGGA ACCCGACCAA GCAGCACTCA 
ACCACGCTAG AGTCCTACCT GATCAAACCA GTGCAGAGAG TGCTGAAATA CCCCCTGCTG 
CTCCGCGAGC TGGTGTCTCT GACGGACACG GACAGTGAGG AGCACTACCA CCTCACAGAG 
GCGCTTAAGG AAATGGAAAA GGTCGCAAGC CATATAAACG AAATGCAGAA GATCTACGAA 
GACTACGGTG CAGTGTTTGA CCAGCTGGTG GCAGAGCAAA GTGGCACAGA GAAAGAGGTC 
ACAGAGATAT CTATGGGACA GTTCCTCATG CATTCCTCTG TGATCTGGCT CAACCCGTTC 
CCATCGTTGG GCCGTATGAA AAAGGATCCC GAGCTGACTG TTTTTGTTTT TAAGAGAGCC 
GTGATACTGC TGTACCGGGA GAACAACAAG GTGAGGAAGA AGATGGTGAC TTCAAGATCA 
GCCCACGGAG ATCAGGATTC ATTCAAGTTT CGCTGGCTCA TTCCCCTGTC AGCGCTACAG 
GTCAGACTGG GCAACACTGC AGACACAGAC TGCCTCTGGG AGCTGATTCA CACCAAATCA 
CAAAAGGAGG GCAGGCCTGA GACGGTGTTC CAGTTGTGCA GCAGCACACC AGAGTGCAAA 
GTGAATATCA TCAAGGTGAT GCGCTCCATC TTGCGGGAGA ACCTGCGTCG AAACATGCAA 
AGGGTTGAAC AGTCTGAGAG AACCTGTAAA AAGCGGCTTA CTCCCCTTCG GAAAAGCCTG 
CCAGCTTCGG CTAGGATAGG TTCTTCTCGA GCCTTTTGGC TAGCCAAACA ACCTTCAAAC 
GACAGTCCCG TTCCCAGCAA TGATCCACTG AGGCTGTCTC TAGCAGATTC TGATTCAGCC 
AGTGCTAGCA GTGGAACCAC CAGCAGTGCC AGCGCTCCTC TAGATCCGCC ATCACAGCAG 
CAGAGCGGCC TCAGAGAATC CAGCATAATC AGCGATGAGG ACGAAGACGG CAGCGAGCCG 
GCTGATTGGA CGGATGCTCC CAATGCTATC GAGGTCCAAT TTCGCAAGCT TGGTCTGACC 
GAGGAGACCT CCCAGGCAGC TGCTCCACGT GTGCAGCCGG AGAGCGGAGA AGTGGACACT 
CCAGAAAGGA AGGCTTCTCT AAAGCGAGCT CACTTTGCTG TGGTCAAACG CAAGCCCGCC 
AGAGACACGA ATAGCCTCAG ACGCAGTCAG GTGGCCCTGC TGAACATGCG TAGACACAGC 
CACTCTTTGG ACATCCCAGC GGAGATCGCG GCACATAGTG TAGACCTGAA CTCGCTACTG 
GAGAAAGATT TCAGCGTGCA GAGCCTGACC TCTGTTGTCA ACGAAGACTT CTTCTATGAC 
AATCCCATGG AGCCAAAGAA TGTGACAACT TCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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tiam2a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

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