map3k15 cDNA ORF clone, Danio rerio(zebrafish)

The following map3k15 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the map3k15 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa141223 XM_021470339.1
Latest version!
Danio rerio mitogen-activated protein kinase kinase kinase 15 (map3k15), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa141223 XM_021473792.1
Latest version!
Danio rerio mitogen-activated protein kinase kinase kinase 15 (map3k15), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa141224 XM_021473793.1
Latest version!
Danio rerio mitogen-activated protein kinase kinase kinase 15 (map3k15), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa141224 XM_021470340.1
Latest version!
Danio rerio mitogen-activated protein kinase kinase kinase 15 (map3k15), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa141225 XM_021470341.1
Latest version!
Danio rerio mitogen-activated protein kinase kinase kinase 15 (map3k15), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa141225 XM_021473794.1
Latest version!
Danio rerio mitogen-activated protein kinase kinase kinase 15 (map3k15), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa141226 XM_021470342.1
Latest version!
Danio rerio mitogen-activated protein kinase kinase kinase 15 (map3k15), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa141226 XM_021473797.1
Latest version!
Danio rerio mitogen-activated protein kinase kinase kinase 15 (map3k15), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa141227 XM_021470343.1
Latest version!
Danio rerio mitogen-activated protein kinase kinase kinase 15 (map3k15), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa141227 XM_021473798.1
Latest version!
Danio rerio mitogen-activated protein kinase kinase kinase 15 (map3k15), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa28664 XM_021473796.1
Latest version!
Danio rerio mitogen-activated protein kinase kinase kinase 15 (map3k15), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa28664 XM_009297482.3
Latest version!
Danio rerio mitogen-activated protein kinase kinase kinase 15 (map3k15), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa28663 XM_009297480.3
Latest version!
Danio rerio mitogen-activated protein kinase kinase kinase 15 (map3k15), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa28663 XM_021473795.1
Latest version!
Danio rerio mitogen-activated protein kinase kinase kinase 15 (map3k15), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa49942 XM_009297481.2
Latest version!
Danio rerio mitogen-activated protein kinase kinase kinase 15 (map3k15), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
ODa28664 XM_009297482.2 Danio rerio mitogen-activated protein kinase kinase kinase 15 (map3k15), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa28663 XM_009297480.2 Danio rerio mitogen-activated protein kinase kinase kinase 15 (map3k15), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa141223
    Clone ID Related Accession (Same CDS sequence) XM_021470339.1 , XM_021473792.1
    Accession Version XM_021470339.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4086bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product mitogen-activated protein kinase kinase kinase 15 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAGATCA TGTCCAATCT ACCTACAGGA GAGGCTGGTG GCGACCACAC CAATGCGGTT 
    TCCATGTCTG CGGGCATGGA GAGAGCCGAT GTGACCAGCC CGAGTCCGGT GAGCAAACAG
    AGGTCCCTGC GGGCTGTGTA TGTGCTGAAT GATGGACTGA AGGCAGTGGC TGCGAATAGC
    CCGGAGTCCG GGGCTCTCCA GTGTCTCCAG CGCGCCTGCG ACGCGGAGAG TGCCATATTG
    ACCACAGTCA CCTTCGGACG ATTAGACTTT GGGGAGACCA CTGTGCTTGA TACTTTTTAC
    GATGCAGATA TTGCAGTTGT GGACATGAGT GATGTGTTCA GACAGCCCTC TTTGTTTTAT
    CACCTCGGGG TCCGTGAGAG CTTTGACATG GCCAACAATG TTATCCTCTA CCACGACATG
    GACCCCGACA CAGCACAGTC CCTTAAGGAC ATGGTGGCAC AGAAAAACAC AGCTGCAAGA
    CCAGTTAAAA TAGGCTTCCC TTGGGTGCAG CTGCCTCCCG AATACAGAGG CTCTGGCCTA
    AGAAACAAAG CCTCTAGTGG GAACTACTAT TTCATCCCAT ACCTGATGAC ACCCAACAAC
    GAGTACTTGT GCTGTGAGAA TGTGGCCCAG CGCAGAGCAT CAGAGTACAT GCAGCCCAAC
    TGGGACAACC TTTTAGGACC TCTGTGTGTC CCTTTGGTGG ACCGCTTCGT CAGCCTTCTC
    AAAGACATTC ATGTCACGTC TTGTGCTTCA TACAAAGATG CACTCCTGAA TGACATCCGA
    AAAGCTCGAG ATAAATACCA GGGTGAGGAG CTGGCCAAAG AGCTTTCCCG TATCAAACTC
    CGCATCGACA ACACTGAGGT TTTGACTCAA GATATTGTCA TGAACCTGCT TTTCTCCTAC
    AGAGACATAC AGGATTATGA TGCTATGGTT AAACTGGTGC AGACCATTGA GATGTTGCCC
    ACCTGTGACT TGGCCAACCA GCCCATGATT CAGTTCCACT ATGCTTTTGC GCTCAATAGA
    CGAAACAGCC CTGGTGATCG AGAGCAGGCG CTGCGTGTGA TGTTGCAGGT TCTTCAATCG
    TGTGAACACC CTGCTCCTGA CATGTTCTGC TTGTGTGGCC GCATATATAA AGACATCTTT
    CTAGACTCAG ACTGTAAAGA CACCAAAAAC AGAGACAACG CCATCCAGTG GTATAGAAAA
    GGTTTCGAGC TTCAGCCCAC GCTCTATTCA GGGATTAATC TAGCAGTGCT GCTTATAGTA
    GCCGGGCAGC AGTTCGAGAC TTCCATAGAA CTCAGGAAGA TCGGTGTAAG GTTGAACAGT
    CTGCTGGGCC GTAAAGGCAG TCTGGAGAAG ATGAATAACT ACTGGGACGT TGGACAGTTT
    TTTACTGTCA GCATGCTGGC CAATGACATC CCCAAAGCTG TGCAGGCCGC TGAGAAACTC
    TTCAAGCTCA AACCACCTAT ATGGTACCTG CGGTCAGTGG TCCAAAACCT CCAGCTGATT
    CAGCATTTCA AGAAGCAGAA CACTGAACAC TCGCCTCAAA GAGAGAGACT CAACTTCTGG
    ATGGACATTA TCGTGGAGGC CACGCAGGGA ACCACCAACG GCCTTCGTTT TCCGGTGTTG
    ATTCTGGAGC CCACTAAAGT TTACCAGCCA TCTTATGTAT CGATTAACAG TGAAGCAGAA
    GAGAAGAATG TGTCTATATG GCACGTGTCG CCCGCTGAGA CGAAGGGAAT ACACGAGTGG
    AACTTCACTG CGTCGTCCAT TAAAGGCATC AGTATCTCAA AGTTTGATGA GCGCTGCTGC
    TTTCTGTACG TCCATGATAA TTCAGATGAC TTCCAGATCT ACTTCTCCAC AGAGGACCAG
    TGCGGCAGGT TCTGCTCAAT GGTGAAAGAG CTCATCACAG ATGGATCTGG AAATGCTGTT
    GAGCTGGAGG GCGAAGGAGA CGGAGACACC TTGGAGTATG AGTATGACTA CAATGAGAAC
    GGTGACCGGG TGGTTCTGGG CAGGGGGACC TATGGAGTTG TCTACGCAGG GAGAGACCTG
    AGCAATCAGG TGCGCATTGC CATCAAGGAG ATCCCTGAGA GAGACAGCAG GTATTCCCAA
    CCACTGCATG AGGAAATCGC CCTTCACAAG TACCTGAAAC ACAGAAACAT AGTTCAGTAC
    CTGGGCTCAG TGTCAGAGGA TGGTTATATC AAGATCTTCA TGGAGCAGGT CCCTGGAGGA
    AGTCTGTCAG CGTTGTTGCG TTCAAAGTGG GGTCCTTTGA AAGAAGCCAC CATAATCTTT
    TACACTCGGC AGATCCTGGA AGGCATTCGT TATTTGCATG AAAACCAAAT AGTTCATCGG
    GATATCAAAG GAGACAATGT TTTAGTCAAC ACTTACAGTG GCGTGTTGAA AATCTCCGAC
    TTCGGCACGT CAAAGAGACT GGCTGGAGTC AATCCCTGCA CTGAGACCTT CACTGGCACC
    TTGCAGTATA TGGCCCCTGA GATCATCGAT AAGGGACCCA GAGGTTATGG GGCCCCTGCT
    GACATCTGGT CTTTGGGCTG CACCATCATT GAGATGGCGA CAGGCAAACC TCCTTTTCAT
    GAGCTGGGAG AGCCACAGGC CGCCATGTTT AAGGTGGGCA TGTTTAAGAT CCATCCGGAG
    ATCCCGGAGT CTCTTTCGTC GGAGGCAAAA TCCTTCATCC TGTCCAGCTT TGAGCCTGAT
    CCCAATAAAC GAGCAATGGC CGGCGACCTG CTCAAAGACC CCTTCCTGCG GCAGAACATC
    AAAGGCAAGA AGAACAAGAT CGCTTTCAAA CCCTCAGTGT GGCAAGACTA CATCCGCAGT
    GTTTCTCTAC CCGTCCAGCT GCAGACAGAA GCCACAGGCA GCAGCAGCAG CGAGCCGGGC
    TCCGTCTCTC CAGACTGCGA CTCCAAACAA GATGTGTTTT TCGAGAAGAA GAAGCGTTCA
    AGCTCCGAAA ACCTCATTAA ACCACCCAGC TCAACCTTTC TCAGTGTTCC AGATGAGAGT
    CCGACAGCAG AAGACCGTAG CTCTCCAGCC TCCTCTGAGA ACAGCGACTC TGGTCTCTTC
    CTGCTCAAGA AAGACAGTGA AAGACGGGCC ATTCTCTACA AGGTCCTCAA TGAAGACCAG
    GATAAAGTAA CCTCCAACCT CATGGAGAAC CACATCCAGG GTCAAGAGGA GCTGAAGTTG
    TCTGTGGATC ACATTAAACA AATCATCTGC ATCTTGAGAG ACTTCATACG CTGCCCTGAA
    CGTCGTGTTA TGGCCACCAC CATCTCAAAA CTCAAACTGG ACCTGGACTT TGATAGCACA
    TCGATCAACC AGATTCAACT CGTTCTGTTC GGCTTTCAGG ACTCTGTGAA TAAAGTGTTA
    CGGAACCACC ACATTAAACC ACACTGGATG TTTGCCATGG ATAACATCAT CAGACGGGCT
    GTGCAGGCAG CTGTTACCAT TTTAATTCCA GAGCTGCAGA CACATTTTGG TCCCGCATCA
    GAGAGCGAGG GCGCAGATAA AGACGCAGAT GAAGTAGATG AAGAGGACGA TGCAGACTTT
    GGATCAGTAG CACGTACAAC ACAGGAAGAT CAAGTTCTCA CATCTGGAGT CAGCACACTT
    AGTTCAGTCA TATCCCATGA TGCGCAGCGG CTACAGCACC CCCTAGGAGC ACAGCTGGGA
    CGACTCAAAC AGGAAACTTG CAGGCTTCTG GAGGAGCTGG TGCAGAAGGA GAGAGAATAT
    CAGCAGGTTC TGAGACAAAC TCTCCAACAG CGGGTACATG ACATCGAGCT TATCAGGATC
    AGACAGCAGC CTGCTGCAGC AGAGATGCCC ACTACACCCT CAGTATTTCA CGTTACTGCA
    CAACCCGAGC CTGACAAGAA GCTGACGGAT TGGCTGAAGG AGCAGGGAGC AGACAGCGAG
    ACGATAGACA AGTTTGTTGC AGAAGATTAT TCACTCAGCG ACGTTCTCTG CGATATTACT
    AAAGAAGATC TTCGATGCCT GAGGCTTAGG GGTGGAATGC TGTGTCGGAT CTGGAAAGCC
    ATTCAACAAC ATCGCAACAA AGGACTTCGA AAGGTCAAAA ACGACGAGGG AAATTCAGAA
    GGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326014.1
    CDS781..4866
    Translation

    Target ORF information:

    RefSeq Version XM_021470339.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio mitogen-activated protein kinase kinase kinase 15 (map3k15), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021470339.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAGATCA TGTCCAATCT ACCTACAGGA GAGGCTGGTG GCGACCACAC CAATGCGGTT 
    TCCATGTCTG CGGGCATGGA GAGAGCCGAT GTGACCAGCC CGAGTCCGGT GAGCAAACAG
    AGGTCCCTGC GGGCTGTGTA TGTGCTGAAT GATGGACTGA AGGCAGTGGC TGCGAATAGC
    CCGGAGTCCG GGGCTCTCCA GTGTCTCCAG CGCGCCTGCG ACGCGGAGAG TGCCATATTG
    ACCACAGTCA CCTTCGGACG ATTAGACTTT GGGGAGACCA CTGTGCTTGA TACTTTTTAC
    GATGCAGATA TTGCAGTTGT GGACATGAGT GATGTGTTCA GACAGCCCTC TTTGTTTTAT
    CACCTCGGGG TCCGTGAGAG CTTTGACATG GCCAACAATG TTATCCTCTA CCACGACATG
    GACCCCGACA CAGCACAGTC CCTTAAGGAC ATGGTGGCAC AGAAAAACAC AGCTGCAAGA
    CCAGTTAAAA TAGGCTTCCC TTGGGTGCAG CTGCCTCCCG AATACAGAGG CTCTGGCCTA
    AGAAACAAAG CCTCTAGTGG GAACTACTAT TTCATCCCAT ACCTGATGAC ACCCAACAAC
    GAGTACTTGT GCTGTGAGAA TGTGGCCCAG CGCAGAGCAT CAGAGTACAT GCAGCCCAAC
    TGGGACAACC TTTTAGGACC TCTGTGTGTC CCTTTGGTGG ACCGCTTCGT CAGCCTTCTC
    AAAGACATTC ATGTCACGTC TTGTGCTTCA TACAAAGATG CACTCCTGAA TGACATCCGA
    AAAGCTCGAG ATAAATACCA GGGTGAGGAG CTGGCCAAAG AGCTTTCCCG TATCAAACTC
    CGCATCGACA ACACTGAGGT TTTGACTCAA GATATTGTCA TGAACCTGCT TTTCTCCTAC
    AGAGACATAC AGGATTATGA TGCTATGGTT AAACTGGTGC AGACCATTGA GATGTTGCCC
    ACCTGTGACT TGGCCAACCA GCCCATGATT CAGTTCCACT ATGCTTTTGC GCTCAATAGA
    CGAAACAGCC CTGGTGATCG AGAGCAGGCG CTGCGTGTGA TGTTGCAGGT TCTTCAATCG
    TGTGAACACC CTGCTCCTGA CATGTTCTGC TTGTGTGGCC GCATATATAA AGACATCTTT
    CTAGACTCAG ACTGTAAAGA CACCAAAAAC AGAGACAACG CCATCCAGTG GTATAGAAAA
    GGTTTCGAGC TTCAGCCCAC GCTCTATTCA GGGATTAATC TAGCAGTGCT GCTTATAGTA
    GCCGGGCAGC AGTTCGAGAC TTCCATAGAA CTCAGGAAGA TCGGTGTAAG GTTGAACAGT
    CTGCTGGGCC GTAAAGGCAG TCTGGAGAAG ATGAATAACT ACTGGGACGT TGGACAGTTT
    TTTACTGTCA GCATGCTGGC CAATGACATC CCCAAAGCTG TGCAGGCCGC TGAGAAACTC
    TTCAAGCTCA AACCACCTAT ATGGTACCTG CGGTCAGTGG TCCAAAACCT CCAGCTGATT
    CAGCATTTCA AGAAGCAGAA CACTGAACAC TCGCCTCAAA GAGAGAGACT CAACTTCTGG
    ATGGACATTA TCGTGGAGGC CACGCAGGGA ACCACCAACG GCCTTCGTTT TCCGGTGTTG
    ATTCTGGAGC CCACTAAAGT TTACCAGCCA TCTTATGTAT CGATTAACAG TGAAGCAGAA
    GAGAAGAATG TGTCTATATG GCACGTGTCG CCCGCTGAGA CGAAGGGAAT ACACGAGTGG
    AACTTCACTG CGTCGTCCAT TAAAGGCATC AGTATCTCAA AGTTTGATGA GCGCTGCTGC
    TTTCTGTACG TCCATGATAA TTCAGATGAC TTCCAGATCT ACTTCTCCAC AGAGGACCAG
    TGCGGCAGGT TCTGCTCAAT GGTGAAAGAG CTCATCACAG ATGGATCTGG AAATGCTGTT
    GAGCTGGAGG GCGAAGGAGA CGGAGACACC TTGGAGTATG AGTATGACTA CAATGAGAAC
    GGTGACCGGG TGGTTCTGGG CAGGGGGACC TATGGAGTTG TCTACGCAGG GAGAGACCTG
    AGCAATCAGG TGCGCATTGC CATCAAGGAG ATCCCTGAGA GAGACAGCAG GTATTCCCAA
    CCACTGCATG AGGAAATCGC CCTTCACAAG TACCTGAAAC ACAGAAACAT AGTTCAGTAC
    CTGGGCTCAG TGTCAGAGGA TGGTTATATC AAGATCTTCA TGGAGCAGGT CCCTGGAGGA
    AGTCTGTCAG CGTTGTTGCG TTCAAAGTGG GGTCCTTTGA AAGAAGCCAC CATAATCTTT
    TACACTCGGC AGATCCTGGA AGGCATTCGT TATTTGCATG AAAACCAAAT AGTTCATCGG
    GATATCAAAG GAGACAATGT TTTAGTCAAC ACTTACAGTG GCGTGTTGAA AATCTCCGAC
    TTCGGCACGT CAAAGAGACT GGCTGGAGTC AATCCCTGCA CTGAGACCTT CACTGGCACC
    TTGCAGTATA TGGCCCCTGA GATCATCGAT AAGGGACCCA GAGGTTATGG GGCCCCTGCT
    GACATCTGGT CTTTGGGCTG CACCATCATT GAGATGGCGA CAGGCAAACC TCCTTTTCAT
    GAGCTGGGAG AGCCACAGGC CGCCATGTTT AAGGTGGGCA TGTTTAAGAT CCATCCGGAG
    ATCCCGGAGT CTCTTTCGTC GGAGGCAAAA TCCTTCATCC TGTCCAGCTT TGAGCCTGAT
    CCCAATAAAC GAGCAATGGC CGGCGACCTG CTCAAAGACC CCTTCCTGCG GCAGAACATC
    AAAGGCAAGA AGAACAAGAT CGCTTTCAAA CCCTCAGTGT GGCAAGACTA CATCCGCAGT
    GTTTCTCTAC CCGTCCAGCT GCAGACAGAA GCCACAGGCA GCAGCAGCAG CGAGCCGGGC
    TCCGTCTCTC CAGACTGCGA CTCCAAACAA GATGTGTTTT TCGAGAAGAA GAAGCGTTCA
    AGCTCCGAAA ACCTCATTAA ACCACCCAGC TCAACCTTTC TCAGTGTTCC AGATGAGAGT
    CCGACAGCAG AAGACCGTAG CTCTCCAGCC TCCTCTGAGA ACAGCGACTC TGGTCTCTTC
    CTGCTCAAGA AAGACAGTGA AAGACGGGCC ATTCTCTACA AGGTCCTCAA TGAAGACCAG
    GATAAAGTAA CCTCCAACCT CATGGAGAAC CACATCCAGG GTCAAGAGGA GCTGAAGTTG
    TCTGTGGATC ACATTAAACA AATCATCTGC ATCTTGAGAG ACTTCATACG CTGCCCTGAA
    CGTCGTGTTA TGGCCACCAC CATCTCAAAA CTCAAACTGG ACCTGGACTT TGATAGCACA
    TCGATCAACC AGATTCAACT CGTTCTGTTC GGCTTTCAGG ACTCTGTGAA TAAAGTGTTA
    CGGAACCACC ACATTAAACC ACACTGGATG TTTGCCATGG ATAACATCAT CAGACGGGCT
    GTGCAGGCAG CTGTTACCAT TTTAATTCCA GAGCTGCAGA CACATTTTGG TCCCGCATCA
    GAGAGCGAGG GCGCAGATAA AGACGCAGAT GAAGTAGATG AAGAGGACGA TGCAGACTTT
    GGATCAGTAG CACGTACAAC ACAGGAAGAT CAAGTTCTCA CATCTGGAGT CAGCACACTT
    AGTTCAGTCA TATCCCATGA TGCGCAGCGG CTACAGCACC CCCTAGGAGC ACAGCTGGGA
    CGACTCAAAC AGGAAACTTG CAGGCTTCTG GAGGAGCTGG TGCAGAAGGA GAGAGAATAT
    CAGCAGGTTC TGAGACAAAC TCTCCAACAG CGGGTACATG ACATCGAGCT TATCAGGATC
    AGACAGCAGC CTGCTGCAGC AGAGATGCCC ACTACACCCT CAGTATTTCA CGTTACTGCA
    CAACCCGAGC CTGACAAGAA GCTGACGGAT TGGCTGAAGG AGCAGGGAGC AGACAGCGAG
    ACGATAGACA AGTTTGTTGC AGAAGATTAT TCACTCAGCG ACGTTCTCTG CGATATTACT
    AAAGAAGATC TTCGATGCCT GAGGCTTAGG GGTGGAATGC TGTGTCGGAT CTGGAAAGCC
    ATTCAACAAC ATCGCAACAA AGGACTTCGA AAGGTCAAAA ACGACGAGGG AAATTCAGAA
    GGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa141223
    Clone ID Related Accession (Same CDS sequence) XM_021470339.1 , XM_021473792.1
    Accession Version XM_021473792.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4086bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product mitogen-activated protein kinase kinase kinase 15 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395177.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAGATCA TGTCCAATCT ACCTACAGGA GAGGCTGGTG GCGACCACAC CAATGCGGTT 
    TCCATGTCTG CGGGCATGGA GAGAGCCGAT GTGACCAGCC CGAGTCCGGT GAGCAAACAG
    AGGTCCCTGC GGGCTGTGTA TGTGCTGAAT GATGGACTGA AGGCAGTGGC TGCGAATAGC
    CCGGAGTCCG GGGCTCTCCA GTGTCTCCAG CGCGCCTGCG ACGCGGAGAG TGCCATATTG
    ACCACAGTCA CCTTCGGACG ATTAGACTTT GGGGAGACCA CTGTGCTTGA TACTTTTTAC
    GATGCAGATA TTGCAGTTGT GGACATGAGT GATGTGTTCA GACAGCCCTC TTTGTTTTAT
    CACCTCGGGG TCCGTGAGAG CTTTGACATG GCCAACAATG TTATCCTCTA CCACGACATG
    GACCCCGACA CAGCACAGTC CCTTAAGGAC ATGGTGGCAC AGAAAAACAC AGCTGCAAGA
    CCAGTTAAAA TAGGCTTCCC TTGGGTGCAG CTGCCTCCCG AATACAGAGG CTCTGGCCTA
    AGAAACAAAG CCTCTAGTGG GAACTACTAT TTCATCCCAT ACCTGATGAC ACCCAACAAC
    GAGTACTTGT GCTGTGAGAA TGTGGCCCAG CGCAGAGCAT CAGAGTACAT GCAGCCCAAC
    TGGGACAACC TTTTAGGACC TCTGTGTGTC CCTTTGGTGG ACCGCTTCGT CAGCCTTCTC
    AAAGACATTC ATGTCACGTC TTGTGCTTCA TACAAAGATG CACTCCTGAA TGACATCCGA
    AAAGCTCGAG ATAAATACCA GGGTGAGGAG CTGGCCAAAG AGCTTTCCCG TATCAAACTC
    CGCATCGACA ACACTGAGGT TTTGACTCAA GATATTGTCA TGAACCTGCT TTTCTCCTAC
    AGAGACATAC AGGATTATGA TGCTATGGTT AAACTGGTGC AGACCATTGA GATGTTGCCC
    ACCTGTGACT TGGCCAACCA GCCCATGATT CAGTTCCACT ATGCTTTTGC GCTCAATAGA
    CGAAACAGCC CTGGTGATCG AGAGCAGGCG CTGCGTGTGA TGTTGCAGGT TCTTCAATCG
    TGTGAACACC CTGCTCCTGA CATGTTCTGC TTGTGTGGCC GCATATATAA AGACATCTTT
    CTAGACTCAG ACTGTAAAGA CACCAAAAAC AGAGACAACG CCATCCAGTG GTATAGAAAA
    GGTTTCGAGC TTCAGCCCAC GCTCTATTCA GGGATTAATC TAGCAGTGCT GCTTATAGTA
    GCCGGGCAGC AGTTCGAGAC TTCCATAGAA CTCAGGAAGA TCGGTGTAAG GTTGAACAGT
    CTGCTGGGCC GTAAAGGCAG TCTGGAGAAG ATGAATAACT ACTGGGACGT TGGACAGTTT
    TTTACTGTCA GCATGCTGGC CAATGACATC CCCAAAGCTG TGCAGGCCGC TGAGAAACTC
    TTCAAGCTCA AACCACCTAT ATGGTACCTG CGGTCAGTGG TCCAAAACCT CCAGCTGATT
    CAGCATTTCA AGAAGCAGAA CACTGAACAC TCGCCTCAAA GAGAGAGACT CAACTTCTGG
    ATGGACATTA TCGTGGAGGC CACGCAGGGA ACCACCAACG GCCTTCGTTT TCCGGTGTTG
    ATTCTGGAGC CCACTAAAGT TTACCAGCCA TCTTATGTAT CGATTAACAG TGAAGCAGAA
    GAGAAGAATG TGTCTATATG GCACGTGTCG CCCGCTGAGA CGAAGGGAAT ACACGAGTGG
    AACTTCACTG CGTCGTCCAT TAAAGGCATC AGTATCTCAA AGTTTGATGA GCGCTGCTGC
    TTTCTGTACG TCCATGATAA TTCAGATGAC TTCCAGATCT ACTTCTCCAC AGAGGACCAG
    TGCGGCAGGT TCTGCTCAAT GGTGAAAGAG CTCATCACAG ATGGATCTGG AAATGCTGTT
    GAGCTGGAGG GCGAAGGAGA CGGAGACACC TTGGAGTATG AGTATGACTA CAATGAGAAC
    GGTGACCGGG TGGTTCTGGG CAGGGGGACC TATGGAGTTG TCTACGCAGG GAGAGACCTG
    AGCAATCAGG TGCGCATTGC CATCAAGGAG ATCCCTGAGA GAGACAGCAG GTATTCCCAA
    CCACTGCATG AGGAAATCGC CCTTCACAAG TACCTGAAAC ACAGAAACAT AGTTCAGTAC
    CTGGGCTCAG TGTCAGAGGA TGGTTATATC AAGATCTTCA TGGAGCAGGT CCCTGGAGGA
    AGTCTGTCAG CGTTGTTGCG TTCAAAGTGG GGTCCTTTGA AAGAAGCCAC CATAATCTTT
    TACACTCGGC AGATCCTGGA AGGCATTCGT TATTTGCATG AAAACCAAAT AGTTCATCGG
    GATATCAAAG GAGACAATGT TTTAGTCAAC ACTTACAGTG GCGTGTTGAA AATCTCCGAC
    TTCGGCACGT CAAAGAGACT GGCTGGAGTC AATCCCTGCA CTGAGACCTT CACTGGCACC
    TTGCAGTATA TGGCCCCTGA GATCATCGAT AAGGGACCCA GAGGTTATGG GGCCCCTGCT
    GACATCTGGT CTTTGGGCTG CACCATCATT GAGATGGCGA CAGGCAAACC TCCTTTTCAT
    GAGCTGGGAG AGCCACAGGC CGCCATGTTT AAGGTGGGCA TGTTTAAGAT CCATCCGGAG
    ATCCCGGAGT CTCTTTCGTC GGAGGCAAAA TCCTTCATCC TGTCCAGCTT TGAGCCTGAT
    CCCAATAAAC GAGCAATGGC CGGCGACCTG CTCAAAGACC CCTTCCTGCG GCAGAACATC
    AAAGGCAAGA AGAACAAGAT CGCTTTCAAA CCCTCAGTGT GGCAAGACTA CATCCGCAGT
    GTTTCTCTAC CCGTCCAGCT GCAGACAGAA GCCACAGGCA GCAGCAGCAG CGAGCCGGGC
    TCCGTCTCTC CAGACTGCGA CTCCAAACAA GATGTGTTTT TCGAGAAGAA GAAGCGTTCA
    AGCTCCGAAA ACCTCATTAA ACCACCCAGC TCAACCTTTC TCAGTGTTCC AGATGAGAGT
    CCGACAGCAG AAGACCGTAG CTCTCCAGCC TCCTCTGAGA ACAGCGACTC TGGTCTCTTC
    CTGCTCAAGA AAGACAGTGA AAGACGGGCC ATTCTCTACA AGGTCCTCAA TGAAGACCAG
    GATAAAGTAA CCTCCAACCT CATGGAGAAC CACATCCAGG GTCAAGAGGA GCTGAAGTTG
    TCTGTGGATC ACATTAAACA AATCATCTGC ATCTTGAGAG ACTTCATACG CTGCCCTGAA
    CGTCGTGTTA TGGCCACCAC CATCTCAAAA CTCAAACTGG ACCTGGACTT TGATAGCACA
    TCGATCAACC AGATTCAACT CGTTCTGTTC GGCTTTCAGG ACTCTGTGAA TAAAGTGTTA
    CGGAACCACC ACATTAAACC ACACTGGATG TTTGCCATGG ATAACATCAT CAGACGGGCT
    GTGCAGGCAG CTGTTACCAT TTTAATTCCA GAGCTGCAGA CACATTTTGG TCCCGCATCA
    GAGAGCGAGG GCGCAGATAA AGACGCAGAT GAAGTAGATG AAGAGGACGA TGCAGACTTT
    GGATCAGTAG CACGTACAAC ACAGGAAGAT CAAGTTCTCA CATCTGGAGT CAGCACACTT
    AGTTCAGTCA TATCCCATGA TGCGCAGCGG CTACAGCACC CCCTAGGAGC ACAGCTGGGA
    CGACTCAAAC AGGAAACTTG CAGGCTTCTG GAGGAGCTGG TGCAGAAGGA GAGAGAATAT
    CAGCAGGTTC TGAGACAAAC TCTCCAACAG CGGGTACATG ACATCGAGCT TATCAGGATC
    AGACAGCAGC CTGCTGCAGC AGAGATGCCC ACTACACCCT CAGTATTTCA CGTTACTGCA
    CAACCCGAGC CTGACAAGAA GCTGACGGAT TGGCTGAAGG AGCAGGGAGC AGACAGCGAG
    ACGATAGACA AGTTTGTTGC AGAAGATTAT TCACTCAGCG ACGTTCTCTG CGATATTACT
    AAAGAAGATC TTCGATGCCT GAGGCTTAGG GGTGGAATGC TGTGTCGGAT CTGGAAAGCC
    ATTCAACAAC ATCGCAACAA AGGACTTCGA AAGGTCAAAA ACGACGAGGG AAATTCAGAA
    GGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021329467.1
    CDS781..4866
    Translation

    Target ORF information:

    RefSeq Version XM_021473792.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio mitogen-activated protein kinase kinase kinase 15 (map3k15), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021473792.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAGATCA TGTCCAATCT ACCTACAGGA GAGGCTGGTG GCGACCACAC CAATGCGGTT 
    TCCATGTCTG CGGGCATGGA GAGAGCCGAT GTGACCAGCC CGAGTCCGGT GAGCAAACAG
    AGGTCCCTGC GGGCTGTGTA TGTGCTGAAT GATGGACTGA AGGCAGTGGC TGCGAATAGC
    CCGGAGTCCG GGGCTCTCCA GTGTCTCCAG CGCGCCTGCG ACGCGGAGAG TGCCATATTG
    ACCACAGTCA CCTTCGGACG ATTAGACTTT GGGGAGACCA CTGTGCTTGA TACTTTTTAC
    GATGCAGATA TTGCAGTTGT GGACATGAGT GATGTGTTCA GACAGCCCTC TTTGTTTTAT
    CACCTCGGGG TCCGTGAGAG CTTTGACATG GCCAACAATG TTATCCTCTA CCACGACATG
    GACCCCGACA CAGCACAGTC CCTTAAGGAC ATGGTGGCAC AGAAAAACAC AGCTGCAAGA
    CCAGTTAAAA TAGGCTTCCC TTGGGTGCAG CTGCCTCCCG AATACAGAGG CTCTGGCCTA
    AGAAACAAAG CCTCTAGTGG GAACTACTAT TTCATCCCAT ACCTGATGAC ACCCAACAAC
    GAGTACTTGT GCTGTGAGAA TGTGGCCCAG CGCAGAGCAT CAGAGTACAT GCAGCCCAAC
    TGGGACAACC TTTTAGGACC TCTGTGTGTC CCTTTGGTGG ACCGCTTCGT CAGCCTTCTC
    AAAGACATTC ATGTCACGTC TTGTGCTTCA TACAAAGATG CACTCCTGAA TGACATCCGA
    AAAGCTCGAG ATAAATACCA GGGTGAGGAG CTGGCCAAAG AGCTTTCCCG TATCAAACTC
    CGCATCGACA ACACTGAGGT TTTGACTCAA GATATTGTCA TGAACCTGCT TTTCTCCTAC
    AGAGACATAC AGGATTATGA TGCTATGGTT AAACTGGTGC AGACCATTGA GATGTTGCCC
    ACCTGTGACT TGGCCAACCA GCCCATGATT CAGTTCCACT ATGCTTTTGC GCTCAATAGA
    CGAAACAGCC CTGGTGATCG AGAGCAGGCG CTGCGTGTGA TGTTGCAGGT TCTTCAATCG
    TGTGAACACC CTGCTCCTGA CATGTTCTGC TTGTGTGGCC GCATATATAA AGACATCTTT
    CTAGACTCAG ACTGTAAAGA CACCAAAAAC AGAGACAACG CCATCCAGTG GTATAGAAAA
    GGTTTCGAGC TTCAGCCCAC GCTCTATTCA GGGATTAATC TAGCAGTGCT GCTTATAGTA
    GCCGGGCAGC AGTTCGAGAC TTCCATAGAA CTCAGGAAGA TCGGTGTAAG GTTGAACAGT
    CTGCTGGGCC GTAAAGGCAG TCTGGAGAAG ATGAATAACT ACTGGGACGT TGGACAGTTT
    TTTACTGTCA GCATGCTGGC CAATGACATC CCCAAAGCTG TGCAGGCCGC TGAGAAACTC
    TTCAAGCTCA AACCACCTAT ATGGTACCTG CGGTCAGTGG TCCAAAACCT CCAGCTGATT
    CAGCATTTCA AGAAGCAGAA CACTGAACAC TCGCCTCAAA GAGAGAGACT CAACTTCTGG
    ATGGACATTA TCGTGGAGGC CACGCAGGGA ACCACCAACG GCCTTCGTTT TCCGGTGTTG
    ATTCTGGAGC CCACTAAAGT TTACCAGCCA TCTTATGTAT CGATTAACAG TGAAGCAGAA
    GAGAAGAATG TGTCTATATG GCACGTGTCG CCCGCTGAGA CGAAGGGAAT ACACGAGTGG
    AACTTCACTG CGTCGTCCAT TAAAGGCATC AGTATCTCAA AGTTTGATGA GCGCTGCTGC
    TTTCTGTACG TCCATGATAA TTCAGATGAC TTCCAGATCT ACTTCTCCAC AGAGGACCAG
    TGCGGCAGGT TCTGCTCAAT GGTGAAAGAG CTCATCACAG ATGGATCTGG AAATGCTGTT
    GAGCTGGAGG GCGAAGGAGA CGGAGACACC TTGGAGTATG AGTATGACTA CAATGAGAAC
    GGTGACCGGG TGGTTCTGGG CAGGGGGACC TATGGAGTTG TCTACGCAGG GAGAGACCTG
    AGCAATCAGG TGCGCATTGC CATCAAGGAG ATCCCTGAGA GAGACAGCAG GTATTCCCAA
    CCACTGCATG AGGAAATCGC CCTTCACAAG TACCTGAAAC ACAGAAACAT AGTTCAGTAC
    CTGGGCTCAG TGTCAGAGGA TGGTTATATC AAGATCTTCA TGGAGCAGGT CCCTGGAGGA
    AGTCTGTCAG CGTTGTTGCG TTCAAAGTGG GGTCCTTTGA AAGAAGCCAC CATAATCTTT
    TACACTCGGC AGATCCTGGA AGGCATTCGT TATTTGCATG AAAACCAAAT AGTTCATCGG
    GATATCAAAG GAGACAATGT TTTAGTCAAC ACTTACAGTG GCGTGTTGAA AATCTCCGAC
    TTCGGCACGT CAAAGAGACT GGCTGGAGTC AATCCCTGCA CTGAGACCTT CACTGGCACC
    TTGCAGTATA TGGCCCCTGA GATCATCGAT AAGGGACCCA GAGGTTATGG GGCCCCTGCT
    GACATCTGGT CTTTGGGCTG CACCATCATT GAGATGGCGA CAGGCAAACC TCCTTTTCAT
    GAGCTGGGAG AGCCACAGGC CGCCATGTTT AAGGTGGGCA TGTTTAAGAT CCATCCGGAG
    ATCCCGGAGT CTCTTTCGTC GGAGGCAAAA TCCTTCATCC TGTCCAGCTT TGAGCCTGAT
    CCCAATAAAC GAGCAATGGC CGGCGACCTG CTCAAAGACC CCTTCCTGCG GCAGAACATC
    AAAGGCAAGA AGAACAAGAT CGCTTTCAAA CCCTCAGTGT GGCAAGACTA CATCCGCAGT
    GTTTCTCTAC CCGTCCAGCT GCAGACAGAA GCCACAGGCA GCAGCAGCAG CGAGCCGGGC
    TCCGTCTCTC CAGACTGCGA CTCCAAACAA GATGTGTTTT TCGAGAAGAA GAAGCGTTCA
    AGCTCCGAAA ACCTCATTAA ACCACCCAGC TCAACCTTTC TCAGTGTTCC AGATGAGAGT
    CCGACAGCAG AAGACCGTAG CTCTCCAGCC TCCTCTGAGA ACAGCGACTC TGGTCTCTTC
    CTGCTCAAGA AAGACAGTGA AAGACGGGCC ATTCTCTACA AGGTCCTCAA TGAAGACCAG
    GATAAAGTAA CCTCCAACCT CATGGAGAAC CACATCCAGG GTCAAGAGGA GCTGAAGTTG
    TCTGTGGATC ACATTAAACA AATCATCTGC ATCTTGAGAG ACTTCATACG CTGCCCTGAA
    CGTCGTGTTA TGGCCACCAC CATCTCAAAA CTCAAACTGG ACCTGGACTT TGATAGCACA
    TCGATCAACC AGATTCAACT CGTTCTGTTC GGCTTTCAGG ACTCTGTGAA TAAAGTGTTA
    CGGAACCACC ACATTAAACC ACACTGGATG TTTGCCATGG ATAACATCAT CAGACGGGCT
    GTGCAGGCAG CTGTTACCAT TTTAATTCCA GAGCTGCAGA CACATTTTGG TCCCGCATCA
    GAGAGCGAGG GCGCAGATAA AGACGCAGAT GAAGTAGATG AAGAGGACGA TGCAGACTTT
    GGATCAGTAG CACGTACAAC ACAGGAAGAT CAAGTTCTCA CATCTGGAGT CAGCACACTT
    AGTTCAGTCA TATCCCATGA TGCGCAGCGG CTACAGCACC CCCTAGGAGC ACAGCTGGGA
    CGACTCAAAC AGGAAACTTG CAGGCTTCTG GAGGAGCTGG TGCAGAAGGA GAGAGAATAT
    CAGCAGGTTC TGAGACAAAC TCTCCAACAG CGGGTACATG ACATCGAGCT TATCAGGATC
    AGACAGCAGC CTGCTGCAGC AGAGATGCCC ACTACACCCT CAGTATTTCA CGTTACTGCA
    CAACCCGAGC CTGACAAGAA GCTGACGGAT TGGCTGAAGG AGCAGGGAGC AGACAGCGAG
    ACGATAGACA AGTTTGTTGC AGAAGATTAT TCACTCAGCG ACGTTCTCTG CGATATTACT
    AAAGAAGATC TTCGATGCCT GAGGCTTAGG GGTGGAATGC TGTGTCGGAT CTGGAAAGCC
    ATTCAACAAC ATCGCAACAA AGGACTTCGA AAGGTCAAAA ACGACGAGGG AAATTCAGAA
    GGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa141224
    Clone ID Related Accession (Same CDS sequence) XM_021470340.1 , XM_021473793.1
    Accession Version XM_021470340.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4083bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product mitogen-activated protein kinase kinase kinase 15 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAGATCA TGTCCAATCT ACCTACAGGA GAGGCTGGTG GCGACCACAC CAATGCGGTT 
    TCCATGTCTG CGGGCATGGA GAGAGCCGAT GTGACCAGCC CGAGTCCGGT GAGCAAACAG
    AGGTCCCTGC GGGCTGTGTA TGTGCTGAAT GATGGACTGA AGGCAGTGGC TGCGAATAGC
    CCGGAGTCCG GGGCTCTCCA GTGTCTCCAG CGCGCCTGCG ACGCGGAGAG TGCCATATTG
    ACCACAGTCA CCTTCGGACG ATTAGACTTT GGGGAGACCA CTGTGCTTGA TACTTTTTAC
    GATGCAGATA TTGCAGTTGT GGACATGAGT GATGTGTTCA GACAGCCCTC TTTGTTTTAT
    CACCTCGGGG TCCGTGAGAG CTTTGACATG GCCAACAATG TTATCCTCTA CCACGACATG
    GACCCCGACA CAGCACAGTC CCTTAAGGAC ATGGTGGCAC AGAAAAACAC AGCTGCAAGA
    CCAGTTAAAA TAGGCTTCCC TTGGGTGCAG CTGCCTCCCG AATACAGAGG CTCTGGCCTA
    AGAAACAAAG CCTCTAGTGG GAACTACTAT TTCATCCCAT ACCTGATGAC ACCCAACAAC
    GAGTACTTGT GCTGTGAGAA TGTGGCCCAG CGCAGAGCAT CAGAGTACAT GCAGCCCAAC
    TGGGACAACC TTTTAGGACC TCTGTGTGTC CCTTTGGTGG ACCGCTTCGT CAGCCTTCTC
    AAAGACATTC ATGTCACGTC TTGTGCTTCA TACAAAGATG CACTCCTGAA TGACATCCGA
    AAAGCTCGAG ATAAATACCA GGGTGAGGAG CTGGCCAAAG AGCTTTCCCG TATCAAACTC
    CGCATCGACA ACACTGAGGT TTTGACTCAA GATATTGTCA TGAACCTGCT TTTCTCCTAC
    AGAGACATAC AGGATTATGA TGCTATGGTT AAACTGGTGC AGACCATTGA GATGTTGCCC
    ACCTGTGACT TGGCCAACCA GCCCATGATT CAGTTCCACT ATGCTTTTGC GCTCAATAGA
    CGAAACAGCC CTGGTGATCG AGAGCAGGCG CTGCGTGTGA TGTTGCAGGT TCTTCAATCG
    TGTGAACACC CTGCTCCTGA CATGTTCTGC TTGTGTGGCC GCATATATAA AGACATCTTT
    CTAGACTCAG ACTGTAAAGA CACCAAAAAC AGAGACAACG CCATCCAGTG GTATAGAAAA
    GGTTTCGAGC TTCAGCCCAC GCTCTATTCA GGGATTAATC TAGCAGTGCT GCTTATAGTA
    GCCGGGCAGC AGTTCGAGAC TTCCATAGAA CTCAGGAAGA TCGGTGTAAG GTTGAACAGT
    CTGCTGGGCC GTAAAGGCAG TCTGGAGAAG ATGAATAACT ACTGGGACGT TGGACAGTTT
    TTTACTGTCA GCATGCTGGC CAATGACATC CCCAAAGCTG TGCAGGCCGC TGAGAAACTC
    TTCAAGCTCA AACCACCTAT ATGGTACCTG CGGTCAGTGG TCCAAAACCT CCAGCTGATT
    CAGCATTTCA AGAAGCAGAA CACTGAACAC TCGCCTCAAA GAGAGAGACT CAACTTCTGG
    ATGGACATTA TCGTGGAGGC CACGCAGGGA ACCACCAACG GCCTTCGTTT TCCGGTGTTG
    ATTCTGGAGC CCACTAAAGT TTACCAGCCA TCTTATGTAT CGATTAACAG TGAAGCAGAA
    GAGAAGAATG TGTCTATATG GCACGTGTCG CCCGCTGAGA CGAAGGGAAT ACACGAGTGG
    AACTTCACTG CGTCGTCCAT TAAAGGCATC AGTATCTCAA AGTTTGATGA GCGCTGCTGC
    TTTCTGTACG TCCATGATAA TTCAGATGAC TTCCAGATCT ACTTCTCCAC AGAGGACCAG
    TGCGGCAGGT TCTGCTCAAT GGTGAAAGAG CTCATCACAG ATGGATCTGG AAATGCTGTT
    GAGCTGGAGG GCGAAGGAGA CGGAGACACC TTGGAGTATG AGTATGACTA CAATGAGAAC
    GGTGACCGGG TGGTTCTGGG CAGGGGGACC TATGGAGTTG TCTACGCAGG GAGAGACCTG
    AGCAATCAGG TGCGCATTGC CATCAAGGAG ATCCCTGAGA GAGACAGCAG GTATTCCCAA
    CCACTGCATG AGGAAATCGC CCTTCACAAG TACCTGAAAC ACAGAAACAT AGTTCAGTAC
    CTGGGCTCAG TGTCAGAGGA TGGTTATATC AAGATCTTCA TGGAGCAGGT CCCTGGAGGA
    AGTCTGTCAG CGTTGTTGCG TTCAAAGTGG GGTCCTTTGA AAGAAGCCAC CATAATCTTT
    TACACTCGGC AGATCCTGGA AGGCATTCGT TATTTGCATG AAAACCAAAT AGTTCATCGG
    GATATCAAAG GAGACAATGT TTTAGTCAAC ACTTACAGTG GCGTGTTGAA AATCTCCGAC
    TTCGGCACGT CAAAGAGACT GGCTGGAGTC AATCCCTGCA CTGAGACCTT CACTGGCACC
    TTGCAGTATA TGGCCCCTGA GATCATCGAT AAGGGACCCA GAGGTTATGG GGCCCCTGCT
    GACATCTGGT CTTTGGGCTG CACCATCATT GAGATGGCGA CAGGCAAACC TCCTTTTCAT
    GAGCTGGGAG AGCCACAGGC CGCCATGTTT AAGGTGGGCA TGTTTAAGAT CCATCCGGAG
    ATCCCGGAGT CTCTTTCGTC GGAGGCAAAA TCCTTCATCC TGTCCAGCTT TGAGCCTGAT
    CCCAATAAAC GAGCAATGGC CGGCGACCTG CTCAAAGACC CCTTCCTGCG GCAGAACATC
    AAAGGCAAGA AGAACAAGAT CGCTTTCAAA CCCTCAGTGT GGCAAGACTA CATCCGCAGT
    GTTTCTCTAC CCGTCCAGCT GCAGACAGAA GCCACAGGCA GCAGCAGCAG CGAGCCGGGC
    TCCGTCTCTC CAGACTGCGA CTCCAAACAA GATGTGTTTT TCGAGAAGAA GAAGCGTTCA
    AGCTCCGAAA ACCTCATTAA ACCACCCAGC TCAACCTTTC TCAGTGTTCC AGATGAGAGT
    CCGACAGCAG AAGACCGTAG CTCTCCAGCC TCCTCTGAGA ACAGCGACTC TGGTCTCTTC
    CTGCTCAAGA AAGACAGTGA AAGACGGGCC ATTCTCTACA AGGTCCTCAA TGAAGACCAG
    GATAAAGTAA CCTCCAACCT CATGGAGAAC CACATCCAGG GTCAAGAGGA GCTGAAGTTG
    TCTGTGGATC ACATTAAACA AATCATCTGC ATCTTGAGAG ACTTCATACG CTGCCCTGAA
    CGTCGTGTTA TGGCCACCAC CATCTCAAAA CTCAAACTGG ACCTGGACTT TGATAGCACA
    TCGATCAACC AGATTCAACT CGTTCTGTTC GGCTTTCAGG ACTCTGTGAA TAAAGTGTTA
    CGGAACCACC ACATTAAACC ACACTGGATG TTTGCCATGG ATAACATCAT CAGACGGGCT
    GTGCAGGCAG CTGTTACCAT TTTAATTCCA GAGCTGCAGA CACATTTTGG TCCCGCATCA
    GAGAGCGAGG GCGCAGATAA AGACGCAGAT GAAGTAGATG AAGAGGACGA TGCAGACTTT
    GGATCAGTAG CACGTACAAC ACAGGAAGAT CAAGTTCTCA CATCTGGAGT CAGCACACTT
    AGTTCAGTCA TATCCCATGA TGCGCAGCGG CTACAGCACC CCCTAGGAGC ACAGCTGGGA
    CGACTCAAAC AGGAAACTTG CAGGCTTCTG GAGGAGCTGG TGCAGAAGGA GAGAGAATAT
    CAGCAGGTTC TGAGACAAAC TCTCCAACAG CGGGTACATG ACATCGAGCT TATCAGGATC
    AGACAGCAGC CTGCTGCAGA GATGCCCACT ACACCCTCAG TATTTCACGT TACTGCACAA
    CCCGAGCCTG ACAAGAAGCT GACGGATTGG CTGAAGGAGC AGGGAGCAGA CAGCGAGACG
    ATAGACAAGT TTGTTGCAGA AGATTATTCA CTCAGCGACG TTCTCTGCGA TATTACTAAA
    GAAGATCTTC GATGCCTGAG GCTTAGGGGT GGAATGCTGT GTCGGATCTG GAAAGCCATT
    CAACAACATC GCAACAAAGG ACTTCGAAAG GTCAAAAACG ACGAGGGAAA TTCAGAAGGA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326015.1
    CDS775..4857
    Translation

    Target ORF information:

    RefSeq Version XM_021470340.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio mitogen-activated protein kinase kinase kinase 15 (map3k15), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021470340.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAGATCA TGTCCAATCT ACCTACAGGA GAGGCTGGTG GCGACCACAC CAATGCGGTT 
    TCCATGTCTG CGGGCATGGA GAGAGCCGAT GTGACCAGCC CGAGTCCGGT GAGCAAACAG
    AGGTCCCTGC GGGCTGTGTA TGTGCTGAAT GATGGACTGA AGGCAGTGGC TGCGAATAGC
    CCGGAGTCCG GGGCTCTCCA GTGTCTCCAG CGCGCCTGCG ACGCGGAGAG TGCCATATTG
    ACCACAGTCA CCTTCGGACG ATTAGACTTT GGGGAGACCA CTGTGCTTGA TACTTTTTAC
    GATGCAGATA TTGCAGTTGT GGACATGAGT GATGTGTTCA GACAGCCCTC TTTGTTTTAT
    CACCTCGGGG TCCGTGAGAG CTTTGACATG GCCAACAATG TTATCCTCTA CCACGACATG
    GACCCCGACA CAGCACAGTC CCTTAAGGAC ATGGTGGCAC AGAAAAACAC AGCTGCAAGA
    CCAGTTAAAA TAGGCTTCCC TTGGGTGCAG CTGCCTCCCG AATACAGAGG CTCTGGCCTA
    AGAAACAAAG CCTCTAGTGG GAACTACTAT TTCATCCCAT ACCTGATGAC ACCCAACAAC
    GAGTACTTGT GCTGTGAGAA TGTGGCCCAG CGCAGAGCAT CAGAGTACAT GCAGCCCAAC
    TGGGACAACC TTTTAGGACC TCTGTGTGTC CCTTTGGTGG ACCGCTTCGT CAGCCTTCTC
    AAAGACATTC ATGTCACGTC TTGTGCTTCA TACAAAGATG CACTCCTGAA TGACATCCGA
    AAAGCTCGAG ATAAATACCA GGGTGAGGAG CTGGCCAAAG AGCTTTCCCG TATCAAACTC
    CGCATCGACA ACACTGAGGT TTTGACTCAA GATATTGTCA TGAACCTGCT TTTCTCCTAC
    AGAGACATAC AGGATTATGA TGCTATGGTT AAACTGGTGC AGACCATTGA GATGTTGCCC
    ACCTGTGACT TGGCCAACCA GCCCATGATT CAGTTCCACT ATGCTTTTGC GCTCAATAGA
    CGAAACAGCC CTGGTGATCG AGAGCAGGCG CTGCGTGTGA TGTTGCAGGT TCTTCAATCG
    TGTGAACACC CTGCTCCTGA CATGTTCTGC TTGTGTGGCC GCATATATAA AGACATCTTT
    CTAGACTCAG ACTGTAAAGA CACCAAAAAC AGAGACAACG CCATCCAGTG GTATAGAAAA
    GGTTTCGAGC TTCAGCCCAC GCTCTATTCA GGGATTAATC TAGCAGTGCT GCTTATAGTA
    GCCGGGCAGC AGTTCGAGAC TTCCATAGAA CTCAGGAAGA TCGGTGTAAG GTTGAACAGT
    CTGCTGGGCC GTAAAGGCAG TCTGGAGAAG ATGAATAACT ACTGGGACGT TGGACAGTTT
    TTTACTGTCA GCATGCTGGC CAATGACATC CCCAAAGCTG TGCAGGCCGC TGAGAAACTC
    TTCAAGCTCA AACCACCTAT ATGGTACCTG CGGTCAGTGG TCCAAAACCT CCAGCTGATT
    CAGCATTTCA AGAAGCAGAA CACTGAACAC TCGCCTCAAA GAGAGAGACT CAACTTCTGG
    ATGGACATTA TCGTGGAGGC CACGCAGGGA ACCACCAACG GCCTTCGTTT TCCGGTGTTG
    ATTCTGGAGC CCACTAAAGT TTACCAGCCA TCTTATGTAT CGATTAACAG TGAAGCAGAA
    GAGAAGAATG TGTCTATATG GCACGTGTCG CCCGCTGAGA CGAAGGGAAT ACACGAGTGG
    AACTTCACTG CGTCGTCCAT TAAAGGCATC AGTATCTCAA AGTTTGATGA GCGCTGCTGC
    TTTCTGTACG TCCATGATAA TTCAGATGAC TTCCAGATCT ACTTCTCCAC AGAGGACCAG
    TGCGGCAGGT TCTGCTCAAT GGTGAAAGAG CTCATCACAG ATGGATCTGG AAATGCTGTT
    GAGCTGGAGG GCGAAGGAGA CGGAGACACC TTGGAGTATG AGTATGACTA CAATGAGAAC
    GGTGACCGGG TGGTTCTGGG CAGGGGGACC TATGGAGTTG TCTACGCAGG GAGAGACCTG
    AGCAATCAGG TGCGCATTGC CATCAAGGAG ATCCCTGAGA GAGACAGCAG GTATTCCCAA
    CCACTGCATG AGGAAATCGC CCTTCACAAG TACCTGAAAC ACAGAAACAT AGTTCAGTAC
    CTGGGCTCAG TGTCAGAGGA TGGTTATATC AAGATCTTCA TGGAGCAGGT CCCTGGAGGA
    AGTCTGTCAG CGTTGTTGCG TTCAAAGTGG GGTCCTTTGA AAGAAGCCAC CATAATCTTT
    TACACTCGGC AGATCCTGGA AGGCATTCGT TATTTGCATG AAAACCAAAT AGTTCATCGG
    GATATCAAAG GAGACAATGT TTTAGTCAAC ACTTACAGTG GCGTGTTGAA AATCTCCGAC
    TTCGGCACGT CAAAGAGACT GGCTGGAGTC AATCCCTGCA CTGAGACCTT CACTGGCACC
    TTGCAGTATA TGGCCCCTGA GATCATCGAT AAGGGACCCA GAGGTTATGG GGCCCCTGCT
    GACATCTGGT CTTTGGGCTG CACCATCATT GAGATGGCGA CAGGCAAACC TCCTTTTCAT
    GAGCTGGGAG AGCCACAGGC CGCCATGTTT AAGGTGGGCA TGTTTAAGAT CCATCCGGAG
    ATCCCGGAGT CTCTTTCGTC GGAGGCAAAA TCCTTCATCC TGTCCAGCTT TGAGCCTGAT
    CCCAATAAAC GAGCAATGGC CGGCGACCTG CTCAAAGACC CCTTCCTGCG GCAGAACATC
    AAAGGCAAGA AGAACAAGAT CGCTTTCAAA CCCTCAGTGT GGCAAGACTA CATCCGCAGT
    GTTTCTCTAC CCGTCCAGCT GCAGACAGAA GCCACAGGCA GCAGCAGCAG CGAGCCGGGC
    TCCGTCTCTC CAGACTGCGA CTCCAAACAA GATGTGTTTT TCGAGAAGAA GAAGCGTTCA
    AGCTCCGAAA ACCTCATTAA ACCACCCAGC TCAACCTTTC TCAGTGTTCC AGATGAGAGT
    CCGACAGCAG AAGACCGTAG CTCTCCAGCC TCCTCTGAGA ACAGCGACTC TGGTCTCTTC
    CTGCTCAAGA AAGACAGTGA AAGACGGGCC ATTCTCTACA AGGTCCTCAA TGAAGACCAG
    GATAAAGTAA CCTCCAACCT CATGGAGAAC CACATCCAGG GTCAAGAGGA GCTGAAGTTG
    TCTGTGGATC ACATTAAACA AATCATCTGC ATCTTGAGAG ACTTCATACG CTGCCCTGAA
    CGTCGTGTTA TGGCCACCAC CATCTCAAAA CTCAAACTGG ACCTGGACTT TGATAGCACA
    TCGATCAACC AGATTCAACT CGTTCTGTTC GGCTTTCAGG ACTCTGTGAA TAAAGTGTTA
    CGGAACCACC ACATTAAACC ACACTGGATG TTTGCCATGG ATAACATCAT CAGACGGGCT
    GTGCAGGCAG CTGTTACCAT TTTAATTCCA GAGCTGCAGA CACATTTTGG TCCCGCATCA
    GAGAGCGAGG GCGCAGATAA AGACGCAGAT GAAGTAGATG AAGAGGACGA TGCAGACTTT
    GGATCAGTAG CACGTACAAC ACAGGAAGAT CAAGTTCTCA CATCTGGAGT CAGCACACTT
    AGTTCAGTCA TATCCCATGA TGCGCAGCGG CTACAGCACC CCCTAGGAGC ACAGCTGGGA
    CGACTCAAAC AGGAAACTTG CAGGCTTCTG GAGGAGCTGG TGCAGAAGGA GAGAGAATAT
    CAGCAGGTTC TGAGACAAAC TCTCCAACAG CGGGTACATG ACATCGAGCT TATCAGGATC
    AGACAGCAGC CTGCTGCAGA GATGCCCACT ACACCCTCAG TATTTCACGT TACTGCACAA
    CCCGAGCCTG ACAAGAAGCT GACGGATTGG CTGAAGGAGC AGGGAGCAGA CAGCGAGACG
    ATAGACAAGT TTGTTGCAGA AGATTATTCA CTCAGCGACG TTCTCTGCGA TATTACTAAA
    GAAGATCTTC GATGCCTGAG GCTTAGGGGT GGAATGCTGT GTCGGATCTG GAAAGCCATT
    CAACAACATC GCAACAAAGG ACTTCGAAAG GTCAAAAACG ACGAGGGAAA TTCAGAAGGA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa141224
    Clone ID Related Accession (Same CDS sequence) XM_021470340.1 , XM_021473793.1
    Accession Version XM_021473793.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4083bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product mitogen-activated protein kinase kinase kinase 15 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395177.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAGATCA TGTCCAATCT ACCTACAGGA GAGGCTGGTG GCGACCACAC CAATGCGGTT 
    TCCATGTCTG CGGGCATGGA GAGAGCCGAT GTGACCAGCC CGAGTCCGGT GAGCAAACAG
    AGGTCCCTGC GGGCTGTGTA TGTGCTGAAT GATGGACTGA AGGCAGTGGC TGCGAATAGC
    CCGGAGTCCG GGGCTCTCCA GTGTCTCCAG CGCGCCTGCG ACGCGGAGAG TGCCATATTG
    ACCACAGTCA CCTTCGGACG ATTAGACTTT GGGGAGACCA CTGTGCTTGA TACTTTTTAC
    GATGCAGATA TTGCAGTTGT GGACATGAGT GATGTGTTCA GACAGCCCTC TTTGTTTTAT
    CACCTCGGGG TCCGTGAGAG CTTTGACATG GCCAACAATG TTATCCTCTA CCACGACATG
    GACCCCGACA CAGCACAGTC CCTTAAGGAC ATGGTGGCAC AGAAAAACAC AGCTGCAAGA
    CCAGTTAAAA TAGGCTTCCC TTGGGTGCAG CTGCCTCCCG AATACAGAGG CTCTGGCCTA
    AGAAACAAAG CCTCTAGTGG GAACTACTAT TTCATCCCAT ACCTGATGAC ACCCAACAAC
    GAGTACTTGT GCTGTGAGAA TGTGGCCCAG CGCAGAGCAT CAGAGTACAT GCAGCCCAAC
    TGGGACAACC TTTTAGGACC TCTGTGTGTC CCTTTGGTGG ACCGCTTCGT CAGCCTTCTC
    AAAGACATTC ATGTCACGTC TTGTGCTTCA TACAAAGATG CACTCCTGAA TGACATCCGA
    AAAGCTCGAG ATAAATACCA GGGTGAGGAG CTGGCCAAAG AGCTTTCCCG TATCAAACTC
    CGCATCGACA ACACTGAGGT TTTGACTCAA GATATTGTCA TGAACCTGCT TTTCTCCTAC
    AGAGACATAC AGGATTATGA TGCTATGGTT AAACTGGTGC AGACCATTGA GATGTTGCCC
    ACCTGTGACT TGGCCAACCA GCCCATGATT CAGTTCCACT ATGCTTTTGC GCTCAATAGA
    CGAAACAGCC CTGGTGATCG AGAGCAGGCG CTGCGTGTGA TGTTGCAGGT TCTTCAATCG
    TGTGAACACC CTGCTCCTGA CATGTTCTGC TTGTGTGGCC GCATATATAA AGACATCTTT
    CTAGACTCAG ACTGTAAAGA CACCAAAAAC AGAGACAACG CCATCCAGTG GTATAGAAAA
    GGTTTCGAGC TTCAGCCCAC GCTCTATTCA GGGATTAATC TAGCAGTGCT GCTTATAGTA
    GCCGGGCAGC AGTTCGAGAC TTCCATAGAA CTCAGGAAGA TCGGTGTAAG GTTGAACAGT
    CTGCTGGGCC GTAAAGGCAG TCTGGAGAAG ATGAATAACT ACTGGGACGT TGGACAGTTT
    TTTACTGTCA GCATGCTGGC CAATGACATC CCCAAAGCTG TGCAGGCCGC TGAGAAACTC
    TTCAAGCTCA AACCACCTAT ATGGTACCTG CGGTCAGTGG TCCAAAACCT CCAGCTGATT
    CAGCATTTCA AGAAGCAGAA CACTGAACAC TCGCCTCAAA GAGAGAGACT CAACTTCTGG
    ATGGACATTA TCGTGGAGGC CACGCAGGGA ACCACCAACG GCCTTCGTTT TCCGGTGTTG
    ATTCTGGAGC CCACTAAAGT TTACCAGCCA TCTTATGTAT CGATTAACAG TGAAGCAGAA
    GAGAAGAATG TGTCTATATG GCACGTGTCG CCCGCTGAGA CGAAGGGAAT ACACGAGTGG
    AACTTCACTG CGTCGTCCAT TAAAGGCATC AGTATCTCAA AGTTTGATGA GCGCTGCTGC
    TTTCTGTACG TCCATGATAA TTCAGATGAC TTCCAGATCT ACTTCTCCAC AGAGGACCAG
    TGCGGCAGGT TCTGCTCAAT GGTGAAAGAG CTCATCACAG ATGGATCTGG AAATGCTGTT
    GAGCTGGAGG GCGAAGGAGA CGGAGACACC TTGGAGTATG AGTATGACTA CAATGAGAAC
    GGTGACCGGG TGGTTCTGGG CAGGGGGACC TATGGAGTTG TCTACGCAGG GAGAGACCTG
    AGCAATCAGG TGCGCATTGC CATCAAGGAG ATCCCTGAGA GAGACAGCAG GTATTCCCAA
    CCACTGCATG AGGAAATCGC CCTTCACAAG TACCTGAAAC ACAGAAACAT AGTTCAGTAC
    CTGGGCTCAG TGTCAGAGGA TGGTTATATC AAGATCTTCA TGGAGCAGGT CCCTGGAGGA
    AGTCTGTCAG CGTTGTTGCG TTCAAAGTGG GGTCCTTTGA AAGAAGCCAC CATAATCTTT
    TACACTCGGC AGATCCTGGA AGGCATTCGT TATTTGCATG AAAACCAAAT AGTTCATCGG
    GATATCAAAG GAGACAATGT TTTAGTCAAC ACTTACAGTG GCGTGTTGAA AATCTCCGAC
    TTCGGCACGT CAAAGAGACT GGCTGGAGTC AATCCCTGCA CTGAGACCTT CACTGGCACC
    TTGCAGTATA TGGCCCCTGA GATCATCGAT AAGGGACCCA GAGGTTATGG GGCCCCTGCT
    GACATCTGGT CTTTGGGCTG CACCATCATT GAGATGGCGA CAGGCAAACC TCCTTTTCAT
    GAGCTGGGAG AGCCACAGGC CGCCATGTTT AAGGTGGGCA TGTTTAAGAT CCATCCGGAG
    ATCCCGGAGT CTCTTTCGTC GGAGGCAAAA TCCTTCATCC TGTCCAGCTT TGAGCCTGAT
    CCCAATAAAC GAGCAATGGC CGGCGACCTG CTCAAAGACC CCTTCCTGCG GCAGAACATC
    AAAGGCAAGA AGAACAAGAT CGCTTTCAAA CCCTCAGTGT GGCAAGACTA CATCCGCAGT
    GTTTCTCTAC CCGTCCAGCT GCAGACAGAA GCCACAGGCA GCAGCAGCAG CGAGCCGGGC
    TCCGTCTCTC CAGACTGCGA CTCCAAACAA GATGTGTTTT TCGAGAAGAA GAAGCGTTCA
    AGCTCCGAAA ACCTCATTAA ACCACCCAGC TCAACCTTTC TCAGTGTTCC AGATGAGAGT
    CCGACAGCAG AAGACCGTAG CTCTCCAGCC TCCTCTGAGA ACAGCGACTC TGGTCTCTTC
    CTGCTCAAGA AAGACAGTGA AAGACGGGCC ATTCTCTACA AGGTCCTCAA TGAAGACCAG
    GATAAAGTAA CCTCCAACCT CATGGAGAAC CACATCCAGG GTCAAGAGGA GCTGAAGTTG
    TCTGTGGATC ACATTAAACA AATCATCTGC ATCTTGAGAG ACTTCATACG CTGCCCTGAA
    CGTCGTGTTA TGGCCACCAC CATCTCAAAA CTCAAACTGG ACCTGGACTT TGATAGCACA
    TCGATCAACC AGATTCAACT CGTTCTGTTC GGCTTTCAGG ACTCTGTGAA TAAAGTGTTA
    CGGAACCACC ACATTAAACC ACACTGGATG TTTGCCATGG ATAACATCAT CAGACGGGCT
    GTGCAGGCAG CTGTTACCAT TTTAATTCCA GAGCTGCAGA CACATTTTGG TCCCGCATCA
    GAGAGCGAGG GCGCAGATAA AGACGCAGAT GAAGTAGATG AAGAGGACGA TGCAGACTTT
    GGATCAGTAG CACGTACAAC ACAGGAAGAT CAAGTTCTCA CATCTGGAGT CAGCACACTT
    AGTTCAGTCA TATCCCATGA TGCGCAGCGG CTACAGCACC CCCTAGGAGC ACAGCTGGGA
    CGACTCAAAC AGGAAACTTG CAGGCTTCTG GAGGAGCTGG TGCAGAAGGA GAGAGAATAT
    CAGCAGGTTC TGAGACAAAC TCTCCAACAG CGGGTACATG ACATCGAGCT TATCAGGATC
    AGACAGCAGC CTGCTGCAGA GATGCCCACT ACACCCTCAG TATTTCACGT TACTGCACAA
    CCCGAGCCTG ACAAGAAGCT GACGGATTGG CTGAAGGAGC AGGGAGCAGA CAGCGAGACG
    ATAGACAAGT TTGTTGCAGA AGATTATTCA CTCAGCGACG TTCTCTGCGA TATTACTAAA
    GAAGATCTTC GATGCCTGAG GCTTAGGGGT GGAATGCTGT GTCGGATCTG GAAAGCCATT
    CAACAACATC GCAACAAAGG ACTTCGAAAG GTCAAAAACG ACGAGGGAAA TTCAGAAGGA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021329468.1
    CDS775..4857
    Translation

    Target ORF information:

    RefSeq Version XM_021473793.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio mitogen-activated protein kinase kinase kinase 15 (map3k15), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021473793.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAGATCA TGTCCAATCT ACCTACAGGA GAGGCTGGTG GCGACCACAC CAATGCGGTT 
    TCCATGTCTG CGGGCATGGA GAGAGCCGAT GTGACCAGCC CGAGTCCGGT GAGCAAACAG
    AGGTCCCTGC GGGCTGTGTA TGTGCTGAAT GATGGACTGA AGGCAGTGGC TGCGAATAGC
    CCGGAGTCCG GGGCTCTCCA GTGTCTCCAG CGCGCCTGCG ACGCGGAGAG TGCCATATTG
    ACCACAGTCA CCTTCGGACG ATTAGACTTT GGGGAGACCA CTGTGCTTGA TACTTTTTAC
    GATGCAGATA TTGCAGTTGT GGACATGAGT GATGTGTTCA GACAGCCCTC TTTGTTTTAT
    CACCTCGGGG TCCGTGAGAG CTTTGACATG GCCAACAATG TTATCCTCTA CCACGACATG
    GACCCCGACA CAGCACAGTC CCTTAAGGAC ATGGTGGCAC AGAAAAACAC AGCTGCAAGA
    CCAGTTAAAA TAGGCTTCCC TTGGGTGCAG CTGCCTCCCG AATACAGAGG CTCTGGCCTA
    AGAAACAAAG CCTCTAGTGG GAACTACTAT TTCATCCCAT ACCTGATGAC ACCCAACAAC
    GAGTACTTGT GCTGTGAGAA TGTGGCCCAG CGCAGAGCAT CAGAGTACAT GCAGCCCAAC
    TGGGACAACC TTTTAGGACC TCTGTGTGTC CCTTTGGTGG ACCGCTTCGT CAGCCTTCTC
    AAAGACATTC ATGTCACGTC TTGTGCTTCA TACAAAGATG CACTCCTGAA TGACATCCGA
    AAAGCTCGAG ATAAATACCA GGGTGAGGAG CTGGCCAAAG AGCTTTCCCG TATCAAACTC
    CGCATCGACA ACACTGAGGT TTTGACTCAA GATATTGTCA TGAACCTGCT TTTCTCCTAC
    AGAGACATAC AGGATTATGA TGCTATGGTT AAACTGGTGC AGACCATTGA GATGTTGCCC
    ACCTGTGACT TGGCCAACCA GCCCATGATT CAGTTCCACT ATGCTTTTGC GCTCAATAGA
    CGAAACAGCC CTGGTGATCG AGAGCAGGCG CTGCGTGTGA TGTTGCAGGT TCTTCAATCG
    TGTGAACACC CTGCTCCTGA CATGTTCTGC TTGTGTGGCC GCATATATAA AGACATCTTT
    CTAGACTCAG ACTGTAAAGA CACCAAAAAC AGAGACAACG CCATCCAGTG GTATAGAAAA
    GGTTTCGAGC TTCAGCCCAC GCTCTATTCA GGGATTAATC TAGCAGTGCT GCTTATAGTA
    GCCGGGCAGC AGTTCGAGAC TTCCATAGAA CTCAGGAAGA TCGGTGTAAG GTTGAACAGT
    CTGCTGGGCC GTAAAGGCAG TCTGGAGAAG ATGAATAACT ACTGGGACGT TGGACAGTTT
    TTTACTGTCA GCATGCTGGC CAATGACATC CCCAAAGCTG TGCAGGCCGC TGAGAAACTC
    TTCAAGCTCA AACCACCTAT ATGGTACCTG CGGTCAGTGG TCCAAAACCT CCAGCTGATT
    CAGCATTTCA AGAAGCAGAA CACTGAACAC TCGCCTCAAA GAGAGAGACT CAACTTCTGG
    ATGGACATTA TCGTGGAGGC CACGCAGGGA ACCACCAACG GCCTTCGTTT TCCGGTGTTG
    ATTCTGGAGC CCACTAAAGT TTACCAGCCA TCTTATGTAT CGATTAACAG TGAAGCAGAA
    GAGAAGAATG TGTCTATATG GCACGTGTCG CCCGCTGAGA CGAAGGGAAT ACACGAGTGG
    AACTTCACTG CGTCGTCCAT TAAAGGCATC AGTATCTCAA AGTTTGATGA GCGCTGCTGC
    TTTCTGTACG TCCATGATAA TTCAGATGAC TTCCAGATCT ACTTCTCCAC AGAGGACCAG
    TGCGGCAGGT TCTGCTCAAT GGTGAAAGAG CTCATCACAG ATGGATCTGG AAATGCTGTT
    GAGCTGGAGG GCGAAGGAGA CGGAGACACC TTGGAGTATG AGTATGACTA CAATGAGAAC
    GGTGACCGGG TGGTTCTGGG CAGGGGGACC TATGGAGTTG TCTACGCAGG GAGAGACCTG
    AGCAATCAGG TGCGCATTGC CATCAAGGAG ATCCCTGAGA GAGACAGCAG GTATTCCCAA
    CCACTGCATG AGGAAATCGC CCTTCACAAG TACCTGAAAC ACAGAAACAT AGTTCAGTAC
    CTGGGCTCAG TGTCAGAGGA TGGTTATATC AAGATCTTCA TGGAGCAGGT CCCTGGAGGA
    AGTCTGTCAG CGTTGTTGCG TTCAAAGTGG GGTCCTTTGA AAGAAGCCAC CATAATCTTT
    TACACTCGGC AGATCCTGGA AGGCATTCGT TATTTGCATG AAAACCAAAT AGTTCATCGG
    GATATCAAAG GAGACAATGT TTTAGTCAAC ACTTACAGTG GCGTGTTGAA AATCTCCGAC
    TTCGGCACGT CAAAGAGACT GGCTGGAGTC AATCCCTGCA CTGAGACCTT CACTGGCACC
    TTGCAGTATA TGGCCCCTGA GATCATCGAT AAGGGACCCA GAGGTTATGG GGCCCCTGCT
    GACATCTGGT CTTTGGGCTG CACCATCATT GAGATGGCGA CAGGCAAACC TCCTTTTCAT
    GAGCTGGGAG AGCCACAGGC CGCCATGTTT AAGGTGGGCA TGTTTAAGAT CCATCCGGAG
    ATCCCGGAGT CTCTTTCGTC GGAGGCAAAA TCCTTCATCC TGTCCAGCTT TGAGCCTGAT
    CCCAATAAAC GAGCAATGGC CGGCGACCTG CTCAAAGACC CCTTCCTGCG GCAGAACATC
    AAAGGCAAGA AGAACAAGAT CGCTTTCAAA CCCTCAGTGT GGCAAGACTA CATCCGCAGT
    GTTTCTCTAC CCGTCCAGCT GCAGACAGAA GCCACAGGCA GCAGCAGCAG CGAGCCGGGC
    TCCGTCTCTC CAGACTGCGA CTCCAAACAA GATGTGTTTT TCGAGAAGAA GAAGCGTTCA
    AGCTCCGAAA ACCTCATTAA ACCACCCAGC TCAACCTTTC TCAGTGTTCC AGATGAGAGT
    CCGACAGCAG AAGACCGTAG CTCTCCAGCC TCCTCTGAGA ACAGCGACTC TGGTCTCTTC
    CTGCTCAAGA AAGACAGTGA AAGACGGGCC ATTCTCTACA AGGTCCTCAA TGAAGACCAG
    GATAAAGTAA CCTCCAACCT CATGGAGAAC CACATCCAGG GTCAAGAGGA GCTGAAGTTG
    TCTGTGGATC ACATTAAACA AATCATCTGC ATCTTGAGAG ACTTCATACG CTGCCCTGAA
    CGTCGTGTTA TGGCCACCAC CATCTCAAAA CTCAAACTGG ACCTGGACTT TGATAGCACA
    TCGATCAACC AGATTCAACT CGTTCTGTTC GGCTTTCAGG ACTCTGTGAA TAAAGTGTTA
    CGGAACCACC ACATTAAACC ACACTGGATG TTTGCCATGG ATAACATCAT CAGACGGGCT
    GTGCAGGCAG CTGTTACCAT TTTAATTCCA GAGCTGCAGA CACATTTTGG TCCCGCATCA
    GAGAGCGAGG GCGCAGATAA AGACGCAGAT GAAGTAGATG AAGAGGACGA TGCAGACTTT
    GGATCAGTAG CACGTACAAC ACAGGAAGAT CAAGTTCTCA CATCTGGAGT CAGCACACTT
    AGTTCAGTCA TATCCCATGA TGCGCAGCGG CTACAGCACC CCCTAGGAGC ACAGCTGGGA
    CGACTCAAAC AGGAAACTTG CAGGCTTCTG GAGGAGCTGG TGCAGAAGGA GAGAGAATAT
    CAGCAGGTTC TGAGACAAAC TCTCCAACAG CGGGTACATG ACATCGAGCT TATCAGGATC
    AGACAGCAGC CTGCTGCAGA GATGCCCACT ACACCCTCAG TATTTCACGT TACTGCACAA
    CCCGAGCCTG ACAAGAAGCT GACGGATTGG CTGAAGGAGC AGGGAGCAGA CAGCGAGACG
    ATAGACAAGT TTGTTGCAGA AGATTATTCA CTCAGCGACG TTCTCTGCGA TATTACTAAA
    GAAGATCTTC GATGCCTGAG GCTTAGGGGT GGAATGCTGT GTCGGATCTG GAAAGCCATT
    CAACAACATC GCAACAAAGG ACTTCGAAAG GTCAAAAACG ACGAGGGAAA TTCAGAAGGA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa141225
    Clone ID Related Accession (Same CDS sequence) XM_021470341.1 , XM_021473794.1
    Accession Version XM_021470341.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4077bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product mitogen-activated protein kinase kinase kinase 15 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGATCA TGTCCAATCT ACCTACAGGA GAGGCTGGTG GCGACCACAC CAATGCGGTT 
    TCCATGTCTG CGGGCATGGA GAGAGCCGAT GTGACCAGCC CGAGTCCGGT GAGCAAACAG
    AGGTCCCTGC GGGCTGTGTA TGTGCTGAAT GATGGACTGA AGGCAGTGGC TGCGAATAGC
    CCGGAGTCCG GGGCTCTCCA GTGTCTCCAG CGCGCCTGCG ACGCGGAGAG TGCCATATTG
    ACCACAGTCA CCTTCGGACG ATTAGACTTT GGGGAGACCA CTGTGCTTGA TACTTTTTAC
    GATGCAGATA TTGCAGTTGT GGACATGAGT GATGTGTTCA GACAGCCCTC TTTGTTTTAT
    CACCTCGGGG TCCGTGAGAG CTTTGACATG GCCAACAATG TTATCCTCTA CCACGACATG
    GACCCCGACA CAGCACAGTC CCTTAAGGAC ATGGTGGCAC AGAAAAACAC AGCTGCAAGA
    CCAGTTAAAA TAGGCTTCCC TTGGGTGCAG CTGCCTCCCG AATACAGAGG CTCTGGCCTA
    AGAAACAAAG CCTCTAGTGG GAACTACTAT TTCATCCCAT ACCTGATGAC ACCCAACAAC
    GAGTACTTGT GCTGTGAGAA TGTGGCCCAG CGCAGAGCAT CAGAGTACAT GCAGCCCAAC
    TGGGACAACC TTTTAGGACC TCTGTGTGTC CCTTTGGTGG ACCGCTTCGT CAGCCTTCTC
    AAAGACATTC ATGTCACGTC TTGTGCTTCA TACAAAGATG CACTCCTGAA TGACATCCGA
    AAAGCTCGAG ATAAATACCA GGGTGAGGAG CTGGCCAAAG AGCTTTCCCG TATCAAACTC
    CGCATCGACA ACACTGAGGT TTTGACTCAA GATATTGTCA TGAACCTGCT TTTCTCCTAC
    AGAGACATAC AGGATTATGA TGCTATGGTT AAACTGGTGC AGACCATTGA GATGTTGCCC
    ACCTGTGACT TGGCCAACCA GCCCATGATT CAGTTCCACT ATGCTTTTGC GCTCAATAGA
    CGAAACAGCC CTGGTGATCG AGAGCAGGCG CTGCGTGTGA TGTTGCAGGT TCTTCAATCG
    TGTGAACACC CTGCTCCTGA CATGTTCTGC TTGTGTGGCC GCATATATAA AGACATCTTT
    CTAGACTCAG ACTGTAAAGA CACCAAAAAC AGAGACAACG CCATCCAGTG GTATAGAAAA
    GGTTTCGAGC TTCAGCCCAC GCTCTATTCA GGGATTAATC TAGCAGTGCT GCTTATAGTA
    GCCGGGCAGC AGTTCGAGAC TTCCATAGAA CTCAGGAAGA TCGGTGTAAG GTTGAACAGT
    CTGCTGGGCC GTAAAGGCAG TCTGGAGAAG ATGAATAACT ACTGGGACGT TGGACAGTTT
    TTTACTGTCA GCATGCTGGC CAATGACATC CCCAAAGCTG TGCAGGCCGC TGAGAAACTC
    TTCAAGCTCA AACCACCTAT ATGGTACCTG CGGTCAGTGG TCCAAAACCT CCAGCTGATT
    CAGCATTTCA AGAAGCAGAA CACTGAACAC TCGCCTCAAA GAGAGAGACT CAACTTCTGG
    ATGGACATTA TCGTGGAGGC CACGCAGGGA ACCACCAACG GCCTTCGTTT TCCGGTGTTG
    ATTCTGGAGC CCACTAAAGT TTACCAGCCA TCTTATGTAT CGATTAACAG TGAAGCAGAA
    GAGAAGAATG TGTCTATATG GCACGTGTCG CCCGCTGAGA CGAAGGGAAT ACACGAGTGG
    AACTTCACTG CGTCGTCCAT TAAAGGCATC AGTATCTCAA AGTTTGATGA GCGCTGCTGC
    TTTCTGTACG TCCATGATAA TTCAGATGAC TTCCAGATCT ACTTCTCCAC AGAGGACCAG
    TGCGGCAGGT TCTGCTCAAT GGTGAAAGAG CTCATCACAG ATGGATCTGG AAATGCTGTT
    GAGCTGGAGG GCGAAGGAGA CGGAGACACC TTGGAGTATG AGTATGACTA CAATGAGAAC
    GGTGACCGGG TGGTTCTGGG CAGGGGGACC TATGGAGTTG TCTACGCAGG GAGAGACCTG
    AGCAATCAGG TGCGCATTGC CATCAAGGAG ATCCCTGAGA GAGACAGCAG GTATTCCCAA
    CCACTGCATG AGGAAATCGC CCTTCACAAG TACCTGAAAC ACAGAAACAT AGTTCAGTAC
    CTGGGCTCAG TGTCAGAGGA TGGTTATATC AAGATCTTCA TGGAGCAGGT CCCTGGAGGA
    AGTCTGTCAG CGTTGTTGCG TTCAAAGTGG GGTCCTTTGA AAGAAGCCAC CATAATCTTT
    TACACTCGGC AGATCCTGGA AGGCATTCGT TATTTGCATG AAAACCAAAT AGTTCATCGG
    GATATCAAAG GAGACAATGT TTTAGTCAAC ACTTACAGTG GCGTGTTGAA AATCTCCGAC
    TTCGGCACGT CAAAGAGACT GGCTGGAGTC AATCCCTGCA CTGAGACCTT CACTGGCACC
    TTGCAGTATA TGGCCCCTGA GATCATCGAT AAGGGACCCA GAGGTTATGG GGCCCCTGCT
    GACATCTGGT CTTTGGGCTG CACCATCATT GAGATGGCGA CAGGCAAACC TCCTTTTCAT
    GAGCTGGGAG AGCCACAGGC CGCCATGTTT AAGGTGGGCA TGTTTAAGAT CCATCCGGAG
    ATCCCGGAGT CTCTTTCGTC GGAGGCAAAA TCCTTCATCC TGTCCAGCTT TGAGCCTGAT
    CCCAATAAAC GAGCAATGGC CGGCGACCTG CTCAAAGACC CCTTCCTGCG GCAGAACATC
    AAAGGCAAGA AGAACAAGAT CGCTTTCAAA CCCTCAGACT ACATCCGCAG TGTTTCTCTA
    CCCGTCCAGC TGCAGACAGA AGCCACAGGC AGCAGCAGCA GCGAGCCGGG CTCCGTCTCT
    CCAGACTGCG ACTCCAAACA AGATGTGTTT TTCGAGAAGA AGAAGCGTTC AAGCTCCGAA
    AACCTCATTA AACCACCCAG CTCAACCTTT CTCAGTGTTC CAGATGAGAG TCCGACAGCA
    GAAGACCGTA GCTCTCCAGC CTCCTCTGAG AACAGCGACT CTGGTCTCTT CCTGCTCAAG
    AAAGACAGTG AAAGACGGGC CATTCTCTAC AAGGTCCTCA ATGAAGACCA GGATAAAGTA
    ACCTCCAACC TCATGGAGAA CCACATCCAG GGTCAAGAGG AGCTGAAGTT GTCTGTGGAT
    CACATTAAAC AAATCATCTG CATCTTGAGA GACTTCATAC GCTGCCCTGA ACGTCGTGTT
    ATGGCCACCA CCATCTCAAA ACTCAAACTG GACCTGGACT TTGATAGCAC ATCGATCAAC
    CAGATTCAAC TCGTTCTGTT CGGCTTTCAG GACTCTGTGA ATAAAGTGTT ACGGAACCAC
    CACATTAAAC CACACTGGAT GTTTGCCATG GATAACATCA TCAGACGGGC TGTGCAGGCA
    GCTGTTACCA TTTTAATTCC AGAGCTGCAG ACACATTTTG GTCCCGCATC AGAGAGCGAG
    GGCGCAGATA AAGACGCAGA TGAAGTAGAT GAAGAGGACG ATGCAGACTT TGGATCAGTA
    GCACGTACAA CACAGGAAGA TCAAGTTCTC ACATCTGGAG TCAGCACACT TAGTTCAGTC
    ATATCCCATG ATGCGCAGCG GCTACAGCAC CCCCTAGGAG CACAGCTGGG ACGACTCAAA
    CAGGAAACTT GCAGGCTTCT GGAGGAGCTG GTGCAGAAGG AGAGAGAATA TCAGCAGGTT
    CTGAGACAAA CTCTCCAACA GCGGGTACAT GACATCGAGC TTATCAGGAT CAGACAGCAG
    CCTGCTGCAG CAGAGATGCC CACTACACCC TCAGTATTTC ACGTTACTGC ACAACCCGAG
    CCTGACAAGA AGCTGACGGA TTGGCTGAAG GAGCAGGGAG CAGACAGCGA GACGATAGAC
    AAGTTTGTTG CAGAAGATTA TTCACTCAGC GACGTTCTCT GCGATATTAC TAAAGAAGAT
    CTTCGATGCC TGAGGCTTAG GGGTGGAATG CTGTGTCGGA TCTGGAAAGC CATTCAACAA
    CATCGCAACA AAGGACTTCG AAAGGTCAAA AACGACGAGG GAAATTCAGA AGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326016.1
    CDS779..4855
    Translation

    Target ORF information:

    RefSeq Version XM_021470341.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio mitogen-activated protein kinase kinase kinase 15 (map3k15), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021470341.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGATCA TGTCCAATCT ACCTACAGGA GAGGCTGGTG GCGACCACAC CAATGCGGTT 
    TCCATGTCTG CGGGCATGGA GAGAGCCGAT GTGACCAGCC CGAGTCCGGT GAGCAAACAG
    AGGTCCCTGC GGGCTGTGTA TGTGCTGAAT GATGGACTGA AGGCAGTGGC TGCGAATAGC
    CCGGAGTCCG GGGCTCTCCA GTGTCTCCAG CGCGCCTGCG ACGCGGAGAG TGCCATATTG
    ACCACAGTCA CCTTCGGACG ATTAGACTTT GGGGAGACCA CTGTGCTTGA TACTTTTTAC
    GATGCAGATA TTGCAGTTGT GGACATGAGT GATGTGTTCA GACAGCCCTC TTTGTTTTAT
    CACCTCGGGG TCCGTGAGAG CTTTGACATG GCCAACAATG TTATCCTCTA CCACGACATG
    GACCCCGACA CAGCACAGTC CCTTAAGGAC ATGGTGGCAC AGAAAAACAC AGCTGCAAGA
    CCAGTTAAAA TAGGCTTCCC TTGGGTGCAG CTGCCTCCCG AATACAGAGG CTCTGGCCTA
    AGAAACAAAG CCTCTAGTGG GAACTACTAT TTCATCCCAT ACCTGATGAC ACCCAACAAC
    GAGTACTTGT GCTGTGAGAA TGTGGCCCAG CGCAGAGCAT CAGAGTACAT GCAGCCCAAC
    TGGGACAACC TTTTAGGACC TCTGTGTGTC CCTTTGGTGG ACCGCTTCGT CAGCCTTCTC
    AAAGACATTC ATGTCACGTC TTGTGCTTCA TACAAAGATG CACTCCTGAA TGACATCCGA
    AAAGCTCGAG ATAAATACCA GGGTGAGGAG CTGGCCAAAG AGCTTTCCCG TATCAAACTC
    CGCATCGACA ACACTGAGGT TTTGACTCAA GATATTGTCA TGAACCTGCT TTTCTCCTAC
    AGAGACATAC AGGATTATGA TGCTATGGTT AAACTGGTGC AGACCATTGA GATGTTGCCC
    ACCTGTGACT TGGCCAACCA GCCCATGATT CAGTTCCACT ATGCTTTTGC GCTCAATAGA
    CGAAACAGCC CTGGTGATCG AGAGCAGGCG CTGCGTGTGA TGTTGCAGGT TCTTCAATCG
    TGTGAACACC CTGCTCCTGA CATGTTCTGC TTGTGTGGCC GCATATATAA AGACATCTTT
    CTAGACTCAG ACTGTAAAGA CACCAAAAAC AGAGACAACG CCATCCAGTG GTATAGAAAA
    GGTTTCGAGC TTCAGCCCAC GCTCTATTCA GGGATTAATC TAGCAGTGCT GCTTATAGTA
    GCCGGGCAGC AGTTCGAGAC TTCCATAGAA CTCAGGAAGA TCGGTGTAAG GTTGAACAGT
    CTGCTGGGCC GTAAAGGCAG TCTGGAGAAG ATGAATAACT ACTGGGACGT TGGACAGTTT
    TTTACTGTCA GCATGCTGGC CAATGACATC CCCAAAGCTG TGCAGGCCGC TGAGAAACTC
    TTCAAGCTCA AACCACCTAT ATGGTACCTG CGGTCAGTGG TCCAAAACCT CCAGCTGATT
    CAGCATTTCA AGAAGCAGAA CACTGAACAC TCGCCTCAAA GAGAGAGACT CAACTTCTGG
    ATGGACATTA TCGTGGAGGC CACGCAGGGA ACCACCAACG GCCTTCGTTT TCCGGTGTTG
    ATTCTGGAGC CCACTAAAGT TTACCAGCCA TCTTATGTAT CGATTAACAG TGAAGCAGAA
    GAGAAGAATG TGTCTATATG GCACGTGTCG CCCGCTGAGA CGAAGGGAAT ACACGAGTGG
    AACTTCACTG CGTCGTCCAT TAAAGGCATC AGTATCTCAA AGTTTGATGA GCGCTGCTGC
    TTTCTGTACG TCCATGATAA TTCAGATGAC TTCCAGATCT ACTTCTCCAC AGAGGACCAG
    TGCGGCAGGT TCTGCTCAAT GGTGAAAGAG CTCATCACAG ATGGATCTGG AAATGCTGTT
    GAGCTGGAGG GCGAAGGAGA CGGAGACACC TTGGAGTATG AGTATGACTA CAATGAGAAC
    GGTGACCGGG TGGTTCTGGG CAGGGGGACC TATGGAGTTG TCTACGCAGG GAGAGACCTG
    AGCAATCAGG TGCGCATTGC CATCAAGGAG ATCCCTGAGA GAGACAGCAG GTATTCCCAA
    CCACTGCATG AGGAAATCGC CCTTCACAAG TACCTGAAAC ACAGAAACAT AGTTCAGTAC
    CTGGGCTCAG TGTCAGAGGA TGGTTATATC AAGATCTTCA TGGAGCAGGT CCCTGGAGGA
    AGTCTGTCAG CGTTGTTGCG TTCAAAGTGG GGTCCTTTGA AAGAAGCCAC CATAATCTTT
    TACACTCGGC AGATCCTGGA AGGCATTCGT TATTTGCATG AAAACCAAAT AGTTCATCGG
    GATATCAAAG GAGACAATGT TTTAGTCAAC ACTTACAGTG GCGTGTTGAA AATCTCCGAC
    TTCGGCACGT CAAAGAGACT GGCTGGAGTC AATCCCTGCA CTGAGACCTT CACTGGCACC
    TTGCAGTATA TGGCCCCTGA GATCATCGAT AAGGGACCCA GAGGTTATGG GGCCCCTGCT
    GACATCTGGT CTTTGGGCTG CACCATCATT GAGATGGCGA CAGGCAAACC TCCTTTTCAT
    GAGCTGGGAG AGCCACAGGC CGCCATGTTT AAGGTGGGCA TGTTTAAGAT CCATCCGGAG
    ATCCCGGAGT CTCTTTCGTC GGAGGCAAAA TCCTTCATCC TGTCCAGCTT TGAGCCTGAT
    CCCAATAAAC GAGCAATGGC CGGCGACCTG CTCAAAGACC CCTTCCTGCG GCAGAACATC
    AAAGGCAAGA AGAACAAGAT CGCTTTCAAA CCCTCAGACT ACATCCGCAG TGTTTCTCTA
    CCCGTCCAGC TGCAGACAGA AGCCACAGGC AGCAGCAGCA GCGAGCCGGG CTCCGTCTCT
    CCAGACTGCG ACTCCAAACA AGATGTGTTT TTCGAGAAGA AGAAGCGTTC AAGCTCCGAA
    AACCTCATTA AACCACCCAG CTCAACCTTT CTCAGTGTTC CAGATGAGAG TCCGACAGCA
    GAAGACCGTA GCTCTCCAGC CTCCTCTGAG AACAGCGACT CTGGTCTCTT CCTGCTCAAG
    AAAGACAGTG AAAGACGGGC CATTCTCTAC AAGGTCCTCA ATGAAGACCA GGATAAAGTA
    ACCTCCAACC TCATGGAGAA CCACATCCAG GGTCAAGAGG AGCTGAAGTT GTCTGTGGAT
    CACATTAAAC AAATCATCTG CATCTTGAGA GACTTCATAC GCTGCCCTGA ACGTCGTGTT
    ATGGCCACCA CCATCTCAAA ACTCAAACTG GACCTGGACT TTGATAGCAC ATCGATCAAC
    CAGATTCAAC TCGTTCTGTT CGGCTTTCAG GACTCTGTGA ATAAAGTGTT ACGGAACCAC
    CACATTAAAC CACACTGGAT GTTTGCCATG GATAACATCA TCAGACGGGC TGTGCAGGCA
    GCTGTTACCA TTTTAATTCC AGAGCTGCAG ACACATTTTG GTCCCGCATC AGAGAGCGAG
    GGCGCAGATA AAGACGCAGA TGAAGTAGAT GAAGAGGACG ATGCAGACTT TGGATCAGTA
    GCACGTACAA CACAGGAAGA TCAAGTTCTC ACATCTGGAG TCAGCACACT TAGTTCAGTC
    ATATCCCATG ATGCGCAGCG GCTACAGCAC CCCCTAGGAG CACAGCTGGG ACGACTCAAA
    CAGGAAACTT GCAGGCTTCT GGAGGAGCTG GTGCAGAAGG AGAGAGAATA TCAGCAGGTT
    CTGAGACAAA CTCTCCAACA GCGGGTACAT GACATCGAGC TTATCAGGAT CAGACAGCAG
    CCTGCTGCAG CAGAGATGCC CACTACACCC TCAGTATTTC ACGTTACTGC ACAACCCGAG
    CCTGACAAGA AGCTGACGGA TTGGCTGAAG GAGCAGGGAG CAGACAGCGA GACGATAGAC
    AAGTTTGTTG CAGAAGATTA TTCACTCAGC GACGTTCTCT GCGATATTAC TAAAGAAGAT
    CTTCGATGCC TGAGGCTTAG GGGTGGAATG CTGTGTCGGA TCTGGAAAGC CATTCAACAA
    CATCGCAACA AAGGACTTCG AAAGGTCAAA AACGACGAGG GAAATTCAGA AGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa141225
    Clone ID Related Accession (Same CDS sequence) XM_021470341.1 , XM_021473794.1
    Accession Version XM_021473794.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4077bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product mitogen-activated protein kinase kinase kinase 15 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395177.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGATCA TGTCCAATCT ACCTACAGGA GAGGCTGGTG GCGACCACAC CAATGCGGTT 
    TCCATGTCTG CGGGCATGGA GAGAGCCGAT GTGACCAGCC CGAGTCCGGT GAGCAAACAG
    AGGTCCCTGC GGGCTGTGTA TGTGCTGAAT GATGGACTGA AGGCAGTGGC TGCGAATAGC
    CCGGAGTCCG GGGCTCTCCA GTGTCTCCAG CGCGCCTGCG ACGCGGAGAG TGCCATATTG
    ACCACAGTCA CCTTCGGACG ATTAGACTTT GGGGAGACCA CTGTGCTTGA TACTTTTTAC
    GATGCAGATA TTGCAGTTGT GGACATGAGT GATGTGTTCA GACAGCCCTC TTTGTTTTAT
    CACCTCGGGG TCCGTGAGAG CTTTGACATG GCCAACAATG TTATCCTCTA CCACGACATG
    GACCCCGACA CAGCACAGTC CCTTAAGGAC ATGGTGGCAC AGAAAAACAC AGCTGCAAGA
    CCAGTTAAAA TAGGCTTCCC TTGGGTGCAG CTGCCTCCCG AATACAGAGG CTCTGGCCTA
    AGAAACAAAG CCTCTAGTGG GAACTACTAT TTCATCCCAT ACCTGATGAC ACCCAACAAC
    GAGTACTTGT GCTGTGAGAA TGTGGCCCAG CGCAGAGCAT CAGAGTACAT GCAGCCCAAC
    TGGGACAACC TTTTAGGACC TCTGTGTGTC CCTTTGGTGG ACCGCTTCGT CAGCCTTCTC
    AAAGACATTC ATGTCACGTC TTGTGCTTCA TACAAAGATG CACTCCTGAA TGACATCCGA
    AAAGCTCGAG ATAAATACCA GGGTGAGGAG CTGGCCAAAG AGCTTTCCCG TATCAAACTC
    CGCATCGACA ACACTGAGGT TTTGACTCAA GATATTGTCA TGAACCTGCT TTTCTCCTAC
    AGAGACATAC AGGATTATGA TGCTATGGTT AAACTGGTGC AGACCATTGA GATGTTGCCC
    ACCTGTGACT TGGCCAACCA GCCCATGATT CAGTTCCACT ATGCTTTTGC GCTCAATAGA
    CGAAACAGCC CTGGTGATCG AGAGCAGGCG CTGCGTGTGA TGTTGCAGGT TCTTCAATCG
    TGTGAACACC CTGCTCCTGA CATGTTCTGC TTGTGTGGCC GCATATATAA AGACATCTTT
    CTAGACTCAG ACTGTAAAGA CACCAAAAAC AGAGACAACG CCATCCAGTG GTATAGAAAA
    GGTTTCGAGC TTCAGCCCAC GCTCTATTCA GGGATTAATC TAGCAGTGCT GCTTATAGTA
    GCCGGGCAGC AGTTCGAGAC TTCCATAGAA CTCAGGAAGA TCGGTGTAAG GTTGAACAGT
    CTGCTGGGCC GTAAAGGCAG TCTGGAGAAG ATGAATAACT ACTGGGACGT TGGACAGTTT
    TTTACTGTCA GCATGCTGGC CAATGACATC CCCAAAGCTG TGCAGGCCGC TGAGAAACTC
    TTCAAGCTCA AACCACCTAT ATGGTACCTG CGGTCAGTGG TCCAAAACCT CCAGCTGATT
    CAGCATTTCA AGAAGCAGAA CACTGAACAC TCGCCTCAAA GAGAGAGACT CAACTTCTGG
    ATGGACATTA TCGTGGAGGC CACGCAGGGA ACCACCAACG GCCTTCGTTT TCCGGTGTTG
    ATTCTGGAGC CCACTAAAGT TTACCAGCCA TCTTATGTAT CGATTAACAG TGAAGCAGAA
    GAGAAGAATG TGTCTATATG GCACGTGTCG CCCGCTGAGA CGAAGGGAAT ACACGAGTGG
    AACTTCACTG CGTCGTCCAT TAAAGGCATC AGTATCTCAA AGTTTGATGA GCGCTGCTGC
    TTTCTGTACG TCCATGATAA TTCAGATGAC TTCCAGATCT ACTTCTCCAC AGAGGACCAG
    TGCGGCAGGT TCTGCTCAAT GGTGAAAGAG CTCATCACAG ATGGATCTGG AAATGCTGTT
    GAGCTGGAGG GCGAAGGAGA CGGAGACACC TTGGAGTATG AGTATGACTA CAATGAGAAC
    GGTGACCGGG TGGTTCTGGG CAGGGGGACC TATGGAGTTG TCTACGCAGG GAGAGACCTG
    AGCAATCAGG TGCGCATTGC CATCAAGGAG ATCCCTGAGA GAGACAGCAG GTATTCCCAA
    CCACTGCATG AGGAAATCGC CCTTCACAAG TACCTGAAAC ACAGAAACAT AGTTCAGTAC
    CTGGGCTCAG TGTCAGAGGA TGGTTATATC AAGATCTTCA TGGAGCAGGT CCCTGGAGGA
    AGTCTGTCAG CGTTGTTGCG TTCAAAGTGG GGTCCTTTGA AAGAAGCCAC CATAATCTTT
    TACACTCGGC AGATCCTGGA AGGCATTCGT TATTTGCATG AAAACCAAAT AGTTCATCGG
    GATATCAAAG GAGACAATGT TTTAGTCAAC ACTTACAGTG GCGTGTTGAA AATCTCCGAC
    TTCGGCACGT CAAAGAGACT GGCTGGAGTC AATCCCTGCA CTGAGACCTT CACTGGCACC
    TTGCAGTATA TGGCCCCTGA GATCATCGAT AAGGGACCCA GAGGTTATGG GGCCCCTGCT
    GACATCTGGT CTTTGGGCTG CACCATCATT GAGATGGCGA CAGGCAAACC TCCTTTTCAT
    GAGCTGGGAG AGCCACAGGC CGCCATGTTT AAGGTGGGCA TGTTTAAGAT CCATCCGGAG
    ATCCCGGAGT CTCTTTCGTC GGAGGCAAAA TCCTTCATCC TGTCCAGCTT TGAGCCTGAT
    CCCAATAAAC GAGCAATGGC CGGCGACCTG CTCAAAGACC CCTTCCTGCG GCAGAACATC
    AAAGGCAAGA AGAACAAGAT CGCTTTCAAA CCCTCAGACT ACATCCGCAG TGTTTCTCTA
    CCCGTCCAGC TGCAGACAGA AGCCACAGGC AGCAGCAGCA GCGAGCCGGG CTCCGTCTCT
    CCAGACTGCG ACTCCAAACA AGATGTGTTT TTCGAGAAGA AGAAGCGTTC AAGCTCCGAA
    AACCTCATTA AACCACCCAG CTCAACCTTT CTCAGTGTTC CAGATGAGAG TCCGACAGCA
    GAAGACCGTA GCTCTCCAGC CTCCTCTGAG AACAGCGACT CTGGTCTCTT CCTGCTCAAG
    AAAGACAGTG AAAGACGGGC CATTCTCTAC AAGGTCCTCA ATGAAGACCA GGATAAAGTA
    ACCTCCAACC TCATGGAGAA CCACATCCAG GGTCAAGAGG AGCTGAAGTT GTCTGTGGAT
    CACATTAAAC AAATCATCTG CATCTTGAGA GACTTCATAC GCTGCCCTGA ACGTCGTGTT
    ATGGCCACCA CCATCTCAAA ACTCAAACTG GACCTGGACT TTGATAGCAC ATCGATCAAC
    CAGATTCAAC TCGTTCTGTT CGGCTTTCAG GACTCTGTGA ATAAAGTGTT ACGGAACCAC
    CACATTAAAC CACACTGGAT GTTTGCCATG GATAACATCA TCAGACGGGC TGTGCAGGCA
    GCTGTTACCA TTTTAATTCC AGAGCTGCAG ACACATTTTG GTCCCGCATC AGAGAGCGAG
    GGCGCAGATA AAGACGCAGA TGAAGTAGAT GAAGAGGACG ATGCAGACTT TGGATCAGTA
    GCACGTACAA CACAGGAAGA TCAAGTTCTC ACATCTGGAG TCAGCACACT TAGTTCAGTC
    ATATCCCATG ATGCGCAGCG GCTACAGCAC CCCCTAGGAG CACAGCTGGG ACGACTCAAA
    CAGGAAACTT GCAGGCTTCT GGAGGAGCTG GTGCAGAAGG AGAGAGAATA TCAGCAGGTT
    CTGAGACAAA CTCTCCAACA GCGGGTACAT GACATCGAGC TTATCAGGAT CAGACAGCAG
    CCTGCTGCAG CAGAGATGCC CACTACACCC TCAGTATTTC ACGTTACTGC ACAACCCGAG
    CCTGACAAGA AGCTGACGGA TTGGCTGAAG GAGCAGGGAG CAGACAGCGA GACGATAGAC
    AAGTTTGTTG CAGAAGATTA TTCACTCAGC GACGTTCTCT GCGATATTAC TAAAGAAGAT
    CTTCGATGCC TGAGGCTTAG GGGTGGAATG CTGTGTCGGA TCTGGAAAGC CATTCAACAA
    CATCGCAACA AAGGACTTCG AAAGGTCAAA AACGACGAGG GAAATTCAGA AGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021329469.1
    CDS778..4854
    Translation

    Target ORF information:

    RefSeq Version XM_021473794.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio mitogen-activated protein kinase kinase kinase 15 (map3k15), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021473794.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGATCA TGTCCAATCT ACCTACAGGA GAGGCTGGTG GCGACCACAC CAATGCGGTT 
    TCCATGTCTG CGGGCATGGA GAGAGCCGAT GTGACCAGCC CGAGTCCGGT GAGCAAACAG
    AGGTCCCTGC GGGCTGTGTA TGTGCTGAAT GATGGACTGA AGGCAGTGGC TGCGAATAGC
    CCGGAGTCCG GGGCTCTCCA GTGTCTCCAG CGCGCCTGCG ACGCGGAGAG TGCCATATTG
    ACCACAGTCA CCTTCGGACG ATTAGACTTT GGGGAGACCA CTGTGCTTGA TACTTTTTAC
    GATGCAGATA TTGCAGTTGT GGACATGAGT GATGTGTTCA GACAGCCCTC TTTGTTTTAT
    CACCTCGGGG TCCGTGAGAG CTTTGACATG GCCAACAATG TTATCCTCTA CCACGACATG
    GACCCCGACA CAGCACAGTC CCTTAAGGAC ATGGTGGCAC AGAAAAACAC AGCTGCAAGA
    CCAGTTAAAA TAGGCTTCCC TTGGGTGCAG CTGCCTCCCG AATACAGAGG CTCTGGCCTA
    AGAAACAAAG CCTCTAGTGG GAACTACTAT TTCATCCCAT ACCTGATGAC ACCCAACAAC
    GAGTACTTGT GCTGTGAGAA TGTGGCCCAG CGCAGAGCAT CAGAGTACAT GCAGCCCAAC
    TGGGACAACC TTTTAGGACC TCTGTGTGTC CCTTTGGTGG ACCGCTTCGT CAGCCTTCTC
    AAAGACATTC ATGTCACGTC TTGTGCTTCA TACAAAGATG CACTCCTGAA TGACATCCGA
    AAAGCTCGAG ATAAATACCA GGGTGAGGAG CTGGCCAAAG AGCTTTCCCG TATCAAACTC
    CGCATCGACA ACACTGAGGT TTTGACTCAA GATATTGTCA TGAACCTGCT TTTCTCCTAC
    AGAGACATAC AGGATTATGA TGCTATGGTT AAACTGGTGC AGACCATTGA GATGTTGCCC
    ACCTGTGACT TGGCCAACCA GCCCATGATT CAGTTCCACT ATGCTTTTGC GCTCAATAGA
    CGAAACAGCC CTGGTGATCG AGAGCAGGCG CTGCGTGTGA TGTTGCAGGT TCTTCAATCG
    TGTGAACACC CTGCTCCTGA CATGTTCTGC TTGTGTGGCC GCATATATAA AGACATCTTT
    CTAGACTCAG ACTGTAAAGA CACCAAAAAC AGAGACAACG CCATCCAGTG GTATAGAAAA
    GGTTTCGAGC TTCAGCCCAC GCTCTATTCA GGGATTAATC TAGCAGTGCT GCTTATAGTA
    GCCGGGCAGC AGTTCGAGAC TTCCATAGAA CTCAGGAAGA TCGGTGTAAG GTTGAACAGT
    CTGCTGGGCC GTAAAGGCAG TCTGGAGAAG ATGAATAACT ACTGGGACGT TGGACAGTTT
    TTTACTGTCA GCATGCTGGC CAATGACATC CCCAAAGCTG TGCAGGCCGC TGAGAAACTC
    TTCAAGCTCA AACCACCTAT ATGGTACCTG CGGTCAGTGG TCCAAAACCT CCAGCTGATT
    CAGCATTTCA AGAAGCAGAA CACTGAACAC TCGCCTCAAA GAGAGAGACT CAACTTCTGG
    ATGGACATTA TCGTGGAGGC CACGCAGGGA ACCACCAACG GCCTTCGTTT TCCGGTGTTG
    ATTCTGGAGC CCACTAAAGT TTACCAGCCA TCTTATGTAT CGATTAACAG TGAAGCAGAA
    GAGAAGAATG TGTCTATATG GCACGTGTCG CCCGCTGAGA CGAAGGGAAT ACACGAGTGG
    AACTTCACTG CGTCGTCCAT TAAAGGCATC AGTATCTCAA AGTTTGATGA GCGCTGCTGC
    TTTCTGTACG TCCATGATAA TTCAGATGAC TTCCAGATCT ACTTCTCCAC AGAGGACCAG
    TGCGGCAGGT TCTGCTCAAT GGTGAAAGAG CTCATCACAG ATGGATCTGG AAATGCTGTT
    GAGCTGGAGG GCGAAGGAGA CGGAGACACC TTGGAGTATG AGTATGACTA CAATGAGAAC
    GGTGACCGGG TGGTTCTGGG CAGGGGGACC TATGGAGTTG TCTACGCAGG GAGAGACCTG
    AGCAATCAGG TGCGCATTGC CATCAAGGAG ATCCCTGAGA GAGACAGCAG GTATTCCCAA
    CCACTGCATG AGGAAATCGC CCTTCACAAG TACCTGAAAC ACAGAAACAT AGTTCAGTAC
    CTGGGCTCAG TGTCAGAGGA TGGTTATATC AAGATCTTCA TGGAGCAGGT CCCTGGAGGA
    AGTCTGTCAG CGTTGTTGCG TTCAAAGTGG GGTCCTTTGA AAGAAGCCAC CATAATCTTT
    TACACTCGGC AGATCCTGGA AGGCATTCGT TATTTGCATG AAAACCAAAT AGTTCATCGG
    GATATCAAAG GAGACAATGT TTTAGTCAAC ACTTACAGTG GCGTGTTGAA AATCTCCGAC
    TTCGGCACGT CAAAGAGACT GGCTGGAGTC AATCCCTGCA CTGAGACCTT CACTGGCACC
    TTGCAGTATA TGGCCCCTGA GATCATCGAT AAGGGACCCA GAGGTTATGG GGCCCCTGCT
    GACATCTGGT CTTTGGGCTG CACCATCATT GAGATGGCGA CAGGCAAACC TCCTTTTCAT
    GAGCTGGGAG AGCCACAGGC CGCCATGTTT AAGGTGGGCA TGTTTAAGAT CCATCCGGAG
    ATCCCGGAGT CTCTTTCGTC GGAGGCAAAA TCCTTCATCC TGTCCAGCTT TGAGCCTGAT
    CCCAATAAAC GAGCAATGGC CGGCGACCTG CTCAAAGACC CCTTCCTGCG GCAGAACATC
    AAAGGCAAGA AGAACAAGAT CGCTTTCAAA CCCTCAGACT ACATCCGCAG TGTTTCTCTA
    CCCGTCCAGC TGCAGACAGA AGCCACAGGC AGCAGCAGCA GCGAGCCGGG CTCCGTCTCT
    CCAGACTGCG ACTCCAAACA AGATGTGTTT TTCGAGAAGA AGAAGCGTTC AAGCTCCGAA
    AACCTCATTA AACCACCCAG CTCAACCTTT CTCAGTGTTC CAGATGAGAG TCCGACAGCA
    GAAGACCGTA GCTCTCCAGC CTCCTCTGAG AACAGCGACT CTGGTCTCTT CCTGCTCAAG
    AAAGACAGTG AAAGACGGGC CATTCTCTAC AAGGTCCTCA ATGAAGACCA GGATAAAGTA
    ACCTCCAACC TCATGGAGAA CCACATCCAG GGTCAAGAGG AGCTGAAGTT GTCTGTGGAT
    CACATTAAAC AAATCATCTG CATCTTGAGA GACTTCATAC GCTGCCCTGA ACGTCGTGTT
    ATGGCCACCA CCATCTCAAA ACTCAAACTG GACCTGGACT TTGATAGCAC ATCGATCAAC
    CAGATTCAAC TCGTTCTGTT CGGCTTTCAG GACTCTGTGA ATAAAGTGTT ACGGAACCAC
    CACATTAAAC CACACTGGAT GTTTGCCATG GATAACATCA TCAGACGGGC TGTGCAGGCA
    GCTGTTACCA TTTTAATTCC AGAGCTGCAG ACACATTTTG GTCCCGCATC AGAGAGCGAG
    GGCGCAGATA AAGACGCAGA TGAAGTAGAT GAAGAGGACG ATGCAGACTT TGGATCAGTA
    GCACGTACAA CACAGGAAGA TCAAGTTCTC ACATCTGGAG TCAGCACACT TAGTTCAGTC
    ATATCCCATG ATGCGCAGCG GCTACAGCAC CCCCTAGGAG CACAGCTGGG ACGACTCAAA
    CAGGAAACTT GCAGGCTTCT GGAGGAGCTG GTGCAGAAGG AGAGAGAATA TCAGCAGGTT
    CTGAGACAAA CTCTCCAACA GCGGGTACAT GACATCGAGC TTATCAGGAT CAGACAGCAG
    CCTGCTGCAG CAGAGATGCC CACTACACCC TCAGTATTTC ACGTTACTGC ACAACCCGAG
    CCTGACAAGA AGCTGACGGA TTGGCTGAAG GAGCAGGGAG CAGACAGCGA GACGATAGAC
    AAGTTTGTTG CAGAAGATTA TTCACTCAGC GACGTTCTCT GCGATATTAC TAAAGAAGAT
    CTTCGATGCC TGAGGCTTAG GGGTGGAATG CTGTGTCGGA TCTGGAAAGC CATTCAACAA
    CATCGCAACA AAGGACTTCG AAAGGTCAAA AACGACGAGG GAAATTCAGA AGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa141226
    Clone ID Related Accession (Same CDS sequence) XM_021470342.1 , XM_021473797.1
    Accession Version XM_021470342.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3027bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product mitogen-activated protein kinase kinase kinase 15 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTTGCAGG TTCTTCAATC GTGTGAACAC CCTGCTCCTG ACATGTTCTG CTTGTGTGGC 
    CGCATATATA AAGACATCTT TCTAGACTCA GACTGTAAAG ACACCAAAAA CAGAGACAAC
    GCCATCCAGT GGTATAGAAA AGGTTTCGAG CTTCAGCCCA CGCTCTATTC AGGGATTAAT
    CTAGCAGTGC TGCTTATAGT AGCCGGGCAG CAGTTCGAGA CTTCCATAGA ACTCAGGAAG
    ATCGGTGTAA GGTTGAACAG TCTGCTGGGC CGTAAAGGCA GTCTGGAGAA GATGAATAAC
    TACTGGGACG TTGGACAGTT TTTTACTGTC AGCATGCTGG CCAATGACAT CCCCAAAGCT
    GTGCAGGCCG CTGAGAAACT CTTCAAGCTC AAACCACCTA TATGGTACCT GCGGTCAGTG
    GTCCAAAACC TCCAGCTGAT TCAGCATTTC AAGAAGCAGA ACACTGAACA CTCGCCTCAA
    AGAGAGAGAC TCAACTTCTG GATGGACATT ATCGTGGAGG CCACGCAGGG AACCACCAAC
    GGCCTTCGTT TTCCGGTGTT GATTCTGGAG CCCACTAAAG TTTACCAGCC ATCTTATGTA
    TCGATTAACA GTGAAGCAGA AGAGAAGAAT GTGTCTATAT GGCACGTGTC GCCCGCTGAG
    ACGAAGGGAA TACACGAGTG GAACTTCACT GCGTCGTCCA TTAAAGGCAT CAGTATCTCA
    AAGTTTGATG AGCGCTGCTG CTTTCTGTAC GTCCATGATA ATTCAGATGA CTTCCAGATC
    TACTTCTCCA CAGAGGACCA GTGCGGCAGG TTCTGCTCAA TGGTGAAAGA GCTCATCACA
    GATGGATCTG GAAATGCTGT TGAGCTGGAG GGCGAAGGAG ACGGAGACAC CTTGGAGTAT
    GAGTATGACT ACAATGAGAA CGGTGACCGG GTGGTTCTGG GCAGGGGGAC CTATGGAGTT
    GTCTACGCAG GGAGAGACCT GAGCAATCAG GTGCGCATTG CCATCAAGGA GATCCCTGAG
    AGAGACAGCA GGTATTCCCA ACCACTGCAT GAGGAAATCG CCCTTCACAA GTACCTGAAA
    CACAGAAACA TAGTTCAGTA CCTGGGCTCA GTGTCAGAGG ATGGTTATAT CAAGATCTTC
    ATGGAGCAGG TCCCTGGAGG AAGTCTGTCA GCGTTGTTGC GTTCAAAGTG GGGTCCTTTG
    AAAGAAGCCA CCATAATCTT TTACACTCGG CAGATCCTGG AAGGCATTCG TTATTTGCAT
    GAAAACCAAA TAGTTCATCG GGATATCAAA GGAGACAATG TTTTAGTCAA CACTTACAGT
    GGCGTGTTGA AAATCTCCGA CTTCGGCACG TCAAAGAGAC TGGCTGGAGT CAATCCCTGC
    ACTGAGACCT TCACTGGCAC CTTGCAGTAT ATGGCCCCTG AGATCATCGA TAAGGGACCC
    AGAGGTTATG GGGCCCCTGC TGACATCTGG TCTTTGGGCT GCACCATCAT TGAGATGGCG
    ACAGGCAAAC CTCCTTTTCA TGAGCTGGGA GAGCCACAGG CCGCCATGTT TAAGGTGGGC
    ATGTTTAAGA TCCATCCGGA GATCCCGGAG TCTCTTTCGT CGGAGGCAAA ATCCTTCATC
    CTGTCCAGCT TTGAGCCTGA TCCCAATAAA CGAGCAATGG CCGGCGACCT GCTCAAAGAC
    CCCTTCCTGC GGCAGAACAT CAAAGGCAAG AAGAACAAGA TCGCTTTCAA ACCCTCAGTG
    TGGCAAGACT ACATCCGCAG TGTTTCTCTA CCCGTCCAGC TGCAGACAGA AGCCACAGGC
    AGCAGCAGCA GCGAGCCGGG CTCCGTCTCT CCAGACTGCG ACTCCAAACA AGATGTGTTT
    TTCGAGAAGA AGAAGCGTTC AAGCTCCGAA AACCTCATTA AACCACCCAG CTCAACCTTT
    CTCAGTGTTC CAGATGAGAG TCCGACAGCA GAAGACCGTA GCTCTCCAGC CTCCTCTGAG
    AACAGCGACT CTGGTCTCTT CCTGCTCAAG AAAGACAGTG AAAGACGGGC CATTCTCTAC
    AAGGTCCTCA ATGAAGACCA GGATAAAGTA ACCTCCAACC TCATGGAGAA CCACATCCAG
    GGTCAAGAGG AGCTGAAGTT GTCTGTGGAT CACATTAAAC AAATCATCTG CATCTTGAGA
    GACTTCATAC GCTGCCCTGA ACGTCGTGTT ATGGCCACCA CCATCTCAAA ACTCAAACTG
    GACCTGGACT TTGATAGCAC ATCGATCAAC CAGATTCAAC TCGTTCTGTT CGGCTTTCAG
    GACTCTGTGA ATAAAGTGTT ACGGAACCAC CACATTAAAC CACACTGGAT GTTTGCCATG
    GATAACATCA TCAGACGGGC TGTGCAGGCA GCTGTTACCA TTTTAATTCC AGAGCTGCAG
    ACACATTTTG GTCCCGCATC AGAGAGCGAG GGCGCAGATA AAGACGCAGA TGAAGTAGAT
    GAAGAGGACG ATGCAGACTT TGGATCAGTA GCACGTACAA CACAGGAAGA TCAAGTTCTC
    ACATCTGGAG TCAGCACACT TAGTTCAGTC ATATCCCATG ATGCGCAGCG GCTACAGCAC
    CCCCTAGGAG CACAGCTGGG ACGACTCAAA CAGGAAACTT GCAGGCTTCT GGAGGAGCTG
    GTGCAGAAGG AGAGAGAATA TCAGCAGGTT CTGAGACAAA CTCTCCAACA GCGGGTACAT
    GACATCGAGC TTATCAGGAT CAGACAGCAG CCTGCTGCAG CAGAGATGCC CACTACACCC
    TCAGTATTTC ACGTTACTGC ACAACCCGAG CCTGACAAGA AGCTGACGGA TTGGCTGAAG
    GAGCAGGGAG CAGACAGCGA GACGATAGAC AAGTTTGTTG CAGAAGATTA TTCACTCAGC
    GACGTTCTCT GCGATATTAC TAAAGAAGAT CTTCGATGCC TGAGGCTTAG GGGTGGAATG
    CTGTGTCGGA TCTGGAAAGC CATTCAACAA CATCGCAACA AAGGACTTCG AAAGGTCAAA
    AACGACGAGG GAAATTCAGA AGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326017.1
    CDS166..3192
    Translation

    Target ORF information:

    RefSeq Version XM_021470342.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio mitogen-activated protein kinase kinase kinase 15 (map3k15), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021470342.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTTGCAGG TTCTTCAATC GTGTGAACAC CCTGCTCCTG ACATGTTCTG CTTGTGTGGC 
    CGCATATATA AAGACATCTT TCTAGACTCA GACTGTAAAG ACACCAAAAA CAGAGACAAC
    GCCATCCAGT GGTATAGAAA AGGTTTCGAG CTTCAGCCCA CGCTCTATTC AGGGATTAAT
    CTAGCAGTGC TGCTTATAGT AGCCGGGCAG CAGTTCGAGA CTTCCATAGA ACTCAGGAAG
    ATCGGTGTAA GGTTGAACAG TCTGCTGGGC CGTAAAGGCA GTCTGGAGAA GATGAATAAC
    TACTGGGACG TTGGACAGTT TTTTACTGTC AGCATGCTGG CCAATGACAT CCCCAAAGCT
    GTGCAGGCCG CTGAGAAACT CTTCAAGCTC AAACCACCTA TATGGTACCT GCGGTCAGTG
    GTCCAAAACC TCCAGCTGAT TCAGCATTTC AAGAAGCAGA ACACTGAACA CTCGCCTCAA
    AGAGAGAGAC TCAACTTCTG GATGGACATT ATCGTGGAGG CCACGCAGGG AACCACCAAC
    GGCCTTCGTT TTCCGGTGTT GATTCTGGAG CCCACTAAAG TTTACCAGCC ATCTTATGTA
    TCGATTAACA GTGAAGCAGA AGAGAAGAAT GTGTCTATAT GGCACGTGTC GCCCGCTGAG
    ACGAAGGGAA TACACGAGTG GAACTTCACT GCGTCGTCCA TTAAAGGCAT CAGTATCTCA
    AAGTTTGATG AGCGCTGCTG CTTTCTGTAC GTCCATGATA ATTCAGATGA CTTCCAGATC
    TACTTCTCCA CAGAGGACCA GTGCGGCAGG TTCTGCTCAA TGGTGAAAGA GCTCATCACA
    GATGGATCTG GAAATGCTGT TGAGCTGGAG GGCGAAGGAG ACGGAGACAC CTTGGAGTAT
    GAGTATGACT ACAATGAGAA CGGTGACCGG GTGGTTCTGG GCAGGGGGAC CTATGGAGTT
    GTCTACGCAG GGAGAGACCT GAGCAATCAG GTGCGCATTG CCATCAAGGA GATCCCTGAG
    AGAGACAGCA GGTATTCCCA ACCACTGCAT GAGGAAATCG CCCTTCACAA GTACCTGAAA
    CACAGAAACA TAGTTCAGTA CCTGGGCTCA GTGTCAGAGG ATGGTTATAT CAAGATCTTC
    ATGGAGCAGG TCCCTGGAGG AAGTCTGTCA GCGTTGTTGC GTTCAAAGTG GGGTCCTTTG
    AAAGAAGCCA CCATAATCTT TTACACTCGG CAGATCCTGG AAGGCATTCG TTATTTGCAT
    GAAAACCAAA TAGTTCATCG GGATATCAAA GGAGACAATG TTTTAGTCAA CACTTACAGT
    GGCGTGTTGA AAATCTCCGA CTTCGGCACG TCAAAGAGAC TGGCTGGAGT CAATCCCTGC
    ACTGAGACCT TCACTGGCAC CTTGCAGTAT ATGGCCCCTG AGATCATCGA TAAGGGACCC
    AGAGGTTATG GGGCCCCTGC TGACATCTGG TCTTTGGGCT GCACCATCAT TGAGATGGCG
    ACAGGCAAAC CTCCTTTTCA TGAGCTGGGA GAGCCACAGG CCGCCATGTT TAAGGTGGGC
    ATGTTTAAGA TCCATCCGGA GATCCCGGAG TCTCTTTCGT CGGAGGCAAA ATCCTTCATC
    CTGTCCAGCT TTGAGCCTGA TCCCAATAAA CGAGCAATGG CCGGCGACCT GCTCAAAGAC
    CCCTTCCTGC GGCAGAACAT CAAAGGCAAG AAGAACAAGA TCGCTTTCAA ACCCTCAGTG
    TGGCAAGACT ACATCCGCAG TGTTTCTCTA CCCGTCCAGC TGCAGACAGA AGCCACAGGC
    AGCAGCAGCA GCGAGCCGGG CTCCGTCTCT CCAGACTGCG ACTCCAAACA AGATGTGTTT
    TTCGAGAAGA AGAAGCGTTC AAGCTCCGAA AACCTCATTA AACCACCCAG CTCAACCTTT
    CTCAGTGTTC CAGATGAGAG TCCGACAGCA GAAGACCGTA GCTCTCCAGC CTCCTCTGAG
    AACAGCGACT CTGGTCTCTT CCTGCTCAAG AAAGACAGTG AAAGACGGGC CATTCTCTAC
    AAGGTCCTCA ATGAAGACCA GGATAAAGTA ACCTCCAACC TCATGGAGAA CCACATCCAG
    GGTCAAGAGG AGCTGAAGTT GTCTGTGGAT CACATTAAAC AAATCATCTG CATCTTGAGA
    GACTTCATAC GCTGCCCTGA ACGTCGTGTT ATGGCCACCA CCATCTCAAA ACTCAAACTG
    GACCTGGACT TTGATAGCAC ATCGATCAAC CAGATTCAAC TCGTTCTGTT CGGCTTTCAG
    GACTCTGTGA ATAAAGTGTT ACGGAACCAC CACATTAAAC CACACTGGAT GTTTGCCATG
    GATAACATCA TCAGACGGGC TGTGCAGGCA GCTGTTACCA TTTTAATTCC AGAGCTGCAG
    ACACATTTTG GTCCCGCATC AGAGAGCGAG GGCGCAGATA AAGACGCAGA TGAAGTAGAT
    GAAGAGGACG ATGCAGACTT TGGATCAGTA GCACGTACAA CACAGGAAGA TCAAGTTCTC
    ACATCTGGAG TCAGCACACT TAGTTCAGTC ATATCCCATG ATGCGCAGCG GCTACAGCAC
    CCCCTAGGAG CACAGCTGGG ACGACTCAAA CAGGAAACTT GCAGGCTTCT GGAGGAGCTG
    GTGCAGAAGG AGAGAGAATA TCAGCAGGTT CTGAGACAAA CTCTCCAACA GCGGGTACAT
    GACATCGAGC TTATCAGGAT CAGACAGCAG CCTGCTGCAG CAGAGATGCC CACTACACCC
    TCAGTATTTC ACGTTACTGC ACAACCCGAG CCTGACAAGA AGCTGACGGA TTGGCTGAAG
    GAGCAGGGAG CAGACAGCGA GACGATAGAC AAGTTTGTTG CAGAAGATTA TTCACTCAGC
    GACGTTCTCT GCGATATTAC TAAAGAAGAT CTTCGATGCC TGAGGCTTAG GGGTGGAATG
    CTGTGTCGGA TCTGGAAAGC CATTCAACAA CATCGCAACA AAGGACTTCG AAAGGTCAAA
    AACGACGAGG GAAATTCAGA AGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa141226
    Clone ID Related Accession (Same CDS sequence) XM_021470342.1 , XM_021473797.1
    Accession Version XM_021473797.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3027bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product mitogen-activated protein kinase kinase kinase 15 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395177.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTTGCAGG TTCTTCAATC GTGTGAACAC CCTGCTCCTG ACATGTTCTG CTTGTGTGGC 
    CGCATATATA AAGACATCTT TCTAGACTCA GACTGTAAAG ACACCAAAAA CAGAGACAAC
    GCCATCCAGT GGTATAGAAA AGGTTTCGAG CTTCAGCCCA CGCTCTATTC AGGGATTAAT
    CTAGCAGTGC TGCTTATAGT AGCCGGGCAG CAGTTCGAGA CTTCCATAGA ACTCAGGAAG
    ATCGGTGTAA GGTTGAACAG TCTGCTGGGC CGTAAAGGCA GTCTGGAGAA GATGAATAAC
    TACTGGGACG TTGGACAGTT TTTTACTGTC AGCATGCTGG CCAATGACAT CCCCAAAGCT
    GTGCAGGCCG CTGAGAAACT CTTCAAGCTC AAACCACCTA TATGGTACCT GCGGTCAGTG
    GTCCAAAACC TCCAGCTGAT TCAGCATTTC AAGAAGCAGA ACACTGAACA CTCGCCTCAA
    AGAGAGAGAC TCAACTTCTG GATGGACATT ATCGTGGAGG CCACGCAGGG AACCACCAAC
    GGCCTTCGTT TTCCGGTGTT GATTCTGGAG CCCACTAAAG TTTACCAGCC ATCTTATGTA
    TCGATTAACA GTGAAGCAGA AGAGAAGAAT GTGTCTATAT GGCACGTGTC GCCCGCTGAG
    ACGAAGGGAA TACACGAGTG GAACTTCACT GCGTCGTCCA TTAAAGGCAT CAGTATCTCA
    AAGTTTGATG AGCGCTGCTG CTTTCTGTAC GTCCATGATA ATTCAGATGA CTTCCAGATC
    TACTTCTCCA CAGAGGACCA GTGCGGCAGG TTCTGCTCAA TGGTGAAAGA GCTCATCACA
    GATGGATCTG GAAATGCTGT TGAGCTGGAG GGCGAAGGAG ACGGAGACAC CTTGGAGTAT
    GAGTATGACT ACAATGAGAA CGGTGACCGG GTGGTTCTGG GCAGGGGGAC CTATGGAGTT
    GTCTACGCAG GGAGAGACCT GAGCAATCAG GTGCGCATTG CCATCAAGGA GATCCCTGAG
    AGAGACAGCA GGTATTCCCA ACCACTGCAT GAGGAAATCG CCCTTCACAA GTACCTGAAA
    CACAGAAACA TAGTTCAGTA CCTGGGCTCA GTGTCAGAGG ATGGTTATAT CAAGATCTTC
    ATGGAGCAGG TCCCTGGAGG AAGTCTGTCA GCGTTGTTGC GTTCAAAGTG GGGTCCTTTG
    AAAGAAGCCA CCATAATCTT TTACACTCGG CAGATCCTGG AAGGCATTCG TTATTTGCAT
    GAAAACCAAA TAGTTCATCG GGATATCAAA GGAGACAATG TTTTAGTCAA CACTTACAGT
    GGCGTGTTGA AAATCTCCGA CTTCGGCACG TCAAAGAGAC TGGCTGGAGT CAATCCCTGC
    ACTGAGACCT TCACTGGCAC CTTGCAGTAT ATGGCCCCTG AGATCATCGA TAAGGGACCC
    AGAGGTTATG GGGCCCCTGC TGACATCTGG TCTTTGGGCT GCACCATCAT TGAGATGGCG
    ACAGGCAAAC CTCCTTTTCA TGAGCTGGGA GAGCCACAGG CCGCCATGTT TAAGGTGGGC
    ATGTTTAAGA TCCATCCGGA GATCCCGGAG TCTCTTTCGT CGGAGGCAAA ATCCTTCATC
    CTGTCCAGCT TTGAGCCTGA TCCCAATAAA CGAGCAATGG CCGGCGACCT GCTCAAAGAC
    CCCTTCCTGC GGCAGAACAT CAAAGGCAAG AAGAACAAGA TCGCTTTCAA ACCCTCAGTG
    TGGCAAGACT ACATCCGCAG TGTTTCTCTA CCCGTCCAGC TGCAGACAGA AGCCACAGGC
    AGCAGCAGCA GCGAGCCGGG CTCCGTCTCT CCAGACTGCG ACTCCAAACA AGATGTGTTT
    TTCGAGAAGA AGAAGCGTTC AAGCTCCGAA AACCTCATTA AACCACCCAG CTCAACCTTT
    CTCAGTGTTC CAGATGAGAG TCCGACAGCA GAAGACCGTA GCTCTCCAGC CTCCTCTGAG
    AACAGCGACT CTGGTCTCTT CCTGCTCAAG AAAGACAGTG AAAGACGGGC CATTCTCTAC
    AAGGTCCTCA ATGAAGACCA GGATAAAGTA ACCTCCAACC TCATGGAGAA CCACATCCAG
    GGTCAAGAGG AGCTGAAGTT GTCTGTGGAT CACATTAAAC AAATCATCTG CATCTTGAGA
    GACTTCATAC GCTGCCCTGA ACGTCGTGTT ATGGCCACCA CCATCTCAAA ACTCAAACTG
    GACCTGGACT TTGATAGCAC ATCGATCAAC CAGATTCAAC TCGTTCTGTT CGGCTTTCAG
    GACTCTGTGA ATAAAGTGTT ACGGAACCAC CACATTAAAC CACACTGGAT GTTTGCCATG
    GATAACATCA TCAGACGGGC TGTGCAGGCA GCTGTTACCA TTTTAATTCC AGAGCTGCAG
    ACACATTTTG GTCCCGCATC AGAGAGCGAG GGCGCAGATA AAGACGCAGA TGAAGTAGAT
    GAAGAGGACG ATGCAGACTT TGGATCAGTA GCACGTACAA CACAGGAAGA TCAAGTTCTC
    ACATCTGGAG TCAGCACACT TAGTTCAGTC ATATCCCATG ATGCGCAGCG GCTACAGCAC
    CCCCTAGGAG CACAGCTGGG ACGACTCAAA CAGGAAACTT GCAGGCTTCT GGAGGAGCTG
    GTGCAGAAGG AGAGAGAATA TCAGCAGGTT CTGAGACAAA CTCTCCAACA GCGGGTACAT
    GACATCGAGC TTATCAGGAT CAGACAGCAG CCTGCTGCAG CAGAGATGCC CACTACACCC
    TCAGTATTTC ACGTTACTGC ACAACCCGAG CCTGACAAGA AGCTGACGGA TTGGCTGAAG
    GAGCAGGGAG CAGACAGCGA GACGATAGAC AAGTTTGTTG CAGAAGATTA TTCACTCAGC
    GACGTTCTCT GCGATATTAC TAAAGAAGAT CTTCGATGCC TGAGGCTTAG GGGTGGAATG
    CTGTGTCGGA TCTGGAAAGC CATTCAACAA CATCGCAACA AAGGACTTCG AAAGGTCAAA
    AACGACGAGG GAAATTCAGA AGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021329472.1
    CDS166..3192
    Translation

    Target ORF information:

    RefSeq Version XM_021473797.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio mitogen-activated protein kinase kinase kinase 15 (map3k15), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021473797.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTTGCAGG TTCTTCAATC GTGTGAACAC CCTGCTCCTG ACATGTTCTG CTTGTGTGGC 
    CGCATATATA AAGACATCTT TCTAGACTCA GACTGTAAAG ACACCAAAAA CAGAGACAAC
    GCCATCCAGT GGTATAGAAA AGGTTTCGAG CTTCAGCCCA CGCTCTATTC AGGGATTAAT
    CTAGCAGTGC TGCTTATAGT AGCCGGGCAG CAGTTCGAGA CTTCCATAGA ACTCAGGAAG
    ATCGGTGTAA GGTTGAACAG TCTGCTGGGC CGTAAAGGCA GTCTGGAGAA GATGAATAAC
    TACTGGGACG TTGGACAGTT TTTTACTGTC AGCATGCTGG CCAATGACAT CCCCAAAGCT
    GTGCAGGCCG CTGAGAAACT CTTCAAGCTC AAACCACCTA TATGGTACCT GCGGTCAGTG
    GTCCAAAACC TCCAGCTGAT TCAGCATTTC AAGAAGCAGA ACACTGAACA CTCGCCTCAA
    AGAGAGAGAC TCAACTTCTG GATGGACATT ATCGTGGAGG CCACGCAGGG AACCACCAAC
    GGCCTTCGTT TTCCGGTGTT GATTCTGGAG CCCACTAAAG TTTACCAGCC ATCTTATGTA
    TCGATTAACA GTGAAGCAGA AGAGAAGAAT GTGTCTATAT GGCACGTGTC GCCCGCTGAG
    ACGAAGGGAA TACACGAGTG GAACTTCACT GCGTCGTCCA TTAAAGGCAT CAGTATCTCA
    AAGTTTGATG AGCGCTGCTG CTTTCTGTAC GTCCATGATA ATTCAGATGA CTTCCAGATC
    TACTTCTCCA CAGAGGACCA GTGCGGCAGG TTCTGCTCAA TGGTGAAAGA GCTCATCACA
    GATGGATCTG GAAATGCTGT TGAGCTGGAG GGCGAAGGAG ACGGAGACAC CTTGGAGTAT
    GAGTATGACT ACAATGAGAA CGGTGACCGG GTGGTTCTGG GCAGGGGGAC CTATGGAGTT
    GTCTACGCAG GGAGAGACCT GAGCAATCAG GTGCGCATTG CCATCAAGGA GATCCCTGAG
    AGAGACAGCA GGTATTCCCA ACCACTGCAT GAGGAAATCG CCCTTCACAA GTACCTGAAA
    CACAGAAACA TAGTTCAGTA CCTGGGCTCA GTGTCAGAGG ATGGTTATAT CAAGATCTTC
    ATGGAGCAGG TCCCTGGAGG AAGTCTGTCA GCGTTGTTGC GTTCAAAGTG GGGTCCTTTG
    AAAGAAGCCA CCATAATCTT TTACACTCGG CAGATCCTGG AAGGCATTCG TTATTTGCAT
    GAAAACCAAA TAGTTCATCG GGATATCAAA GGAGACAATG TTTTAGTCAA CACTTACAGT
    GGCGTGTTGA AAATCTCCGA CTTCGGCACG TCAAAGAGAC TGGCTGGAGT CAATCCCTGC
    ACTGAGACCT TCACTGGCAC CTTGCAGTAT ATGGCCCCTG AGATCATCGA TAAGGGACCC
    AGAGGTTATG GGGCCCCTGC TGACATCTGG TCTTTGGGCT GCACCATCAT TGAGATGGCG
    ACAGGCAAAC CTCCTTTTCA TGAGCTGGGA GAGCCACAGG CCGCCATGTT TAAGGTGGGC
    ATGTTTAAGA TCCATCCGGA GATCCCGGAG TCTCTTTCGT CGGAGGCAAA ATCCTTCATC
    CTGTCCAGCT TTGAGCCTGA TCCCAATAAA CGAGCAATGG CCGGCGACCT GCTCAAAGAC
    CCCTTCCTGC GGCAGAACAT CAAAGGCAAG AAGAACAAGA TCGCTTTCAA ACCCTCAGTG
    TGGCAAGACT ACATCCGCAG TGTTTCTCTA CCCGTCCAGC TGCAGACAGA AGCCACAGGC
    AGCAGCAGCA GCGAGCCGGG CTCCGTCTCT CCAGACTGCG ACTCCAAACA AGATGTGTTT
    TTCGAGAAGA AGAAGCGTTC AAGCTCCGAA AACCTCATTA AACCACCCAG CTCAACCTTT
    CTCAGTGTTC CAGATGAGAG TCCGACAGCA GAAGACCGTA GCTCTCCAGC CTCCTCTGAG
    AACAGCGACT CTGGTCTCTT CCTGCTCAAG AAAGACAGTG AAAGACGGGC CATTCTCTAC
    AAGGTCCTCA ATGAAGACCA GGATAAAGTA ACCTCCAACC TCATGGAGAA CCACATCCAG
    GGTCAAGAGG AGCTGAAGTT GTCTGTGGAT CACATTAAAC AAATCATCTG CATCTTGAGA
    GACTTCATAC GCTGCCCTGA ACGTCGTGTT ATGGCCACCA CCATCTCAAA ACTCAAACTG
    GACCTGGACT TTGATAGCAC ATCGATCAAC CAGATTCAAC TCGTTCTGTT CGGCTTTCAG
    GACTCTGTGA ATAAAGTGTT ACGGAACCAC CACATTAAAC CACACTGGAT GTTTGCCATG
    GATAACATCA TCAGACGGGC TGTGCAGGCA GCTGTTACCA TTTTAATTCC AGAGCTGCAG
    ACACATTTTG GTCCCGCATC AGAGAGCGAG GGCGCAGATA AAGACGCAGA TGAAGTAGAT
    GAAGAGGACG ATGCAGACTT TGGATCAGTA GCACGTACAA CACAGGAAGA TCAAGTTCTC
    ACATCTGGAG TCAGCACACT TAGTTCAGTC ATATCCCATG ATGCGCAGCG GCTACAGCAC
    CCCCTAGGAG CACAGCTGGG ACGACTCAAA CAGGAAACTT GCAGGCTTCT GGAGGAGCTG
    GTGCAGAAGG AGAGAGAATA TCAGCAGGTT CTGAGACAAA CTCTCCAACA GCGGGTACAT
    GACATCGAGC TTATCAGGAT CAGACAGCAG CCTGCTGCAG CAGAGATGCC CACTACACCC
    TCAGTATTTC ACGTTACTGC ACAACCCGAG CCTGACAAGA AGCTGACGGA TTGGCTGAAG
    GAGCAGGGAG CAGACAGCGA GACGATAGAC AAGTTTGTTG CAGAAGATTA TTCACTCAGC
    GACGTTCTCT GCGATATTAC TAAAGAAGAT CTTCGATGCC TGAGGCTTAG GGGTGGAATG
    CTGTGTCGGA TCTGGAAAGC CATTCAACAA CATCGCAACA AAGGACTTCG AAAGGTCAAA
    AACGACGAGG GAAATTCAGA AGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa141227
    Clone ID Related Accession (Same CDS sequence) XM_021473798.1 , XM_021470343.1
    Accession Version XM_021473798.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3912bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product mitogen-activated protein kinase kinase kinase 15 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395177.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAGATCA TGTCCAATCT ACCTACAGGA GAGGCTGGTG GCGACCACAC CAATGCGGTT 
    TCCATGTCTG CGGGCATGGA GAGAGCCGAT GTGACCAGCC CGAGTCCGGT GAGCAAACAG
    AGGTCCCTGC GGGCTGTGTA TGTGCTGAAT GATGGACTGA AGGCAGTGGC TGCGAATAGC
    CCGGAGTCCG GGGCTCTCCA GTGTCTCCAG CGCGCCTGCG ACGCGGAGAG TGCCATATTG
    ACCACAGTCA CCTTCGGACG ATTAGACTTT GGGGAGACCA CTGTGCTTGA TACTTTTTAC
    GATGCAGATA TTGCAGTTGT GGACATGAGT GATGTGTTCA GACAGCCCTC TTTGTTTTAT
    CACCTCGGGG TCCGTGAGAG CTTTGACATG GCCAACAATG TTATCCTCTA CCACGACATG
    GACCCCGACA CAGCACAGTC CCTTAAGGAC ATGGTGGCAC AGAAAAACAC AGCCTCTAGT
    GGGAACTACT ATTTCATCCC ATACCTGATG ACACCCAACA ACGAGTACTT GTGCTGTGAG
    AATGTGGCCC AGCGCAGAGC ATCAGAGTAC ATGCAGCCCA ACTGGGACAA CCTTTTAGGA
    CCTCTGTGTG TCCCTTTGGT GGACCGCTTC GTCAGCCTTC TCAAAGACAT TCATGTCACG
    TCTTGTGCTT CATACAAAGA TGCACTCCTG AATGACATCC GAAAAGCTCG AGATAAATAC
    CAGGGTGAGG AGCTGGCCAA AGAGCTTTCC CGTATCAAAC TCCGCATCGA CAACACTGAG
    GTTTTGACTC AAGATATTGT CATGAACCTG CTTTTCTCCT ACAGAGACAT ACAGGATTAT
    GATGCTATGG TTAAACTGGT GCAGACCATT GAGATGTTGC CCACCTGTGA CTTGGCCAAC
    CAGCCCATGA TTCAGTTCCA CTATGCTTTT GCGCTCAATA GACGAAACAG CCCTGGTGAT
    CGAGAGCAGG CGCTGCGTGT GATGTTGCAG GTTCTTCAAT CGTGTGAACA CCCTGCTCCT
    GACATGTTCT GCTTGTGTGG CCGCATATAT AAAGACATCT TTCTAGACTC AGACTGTAAA
    GACACCAAAA ACAGAGACAA CGCCATCCAG TGGTATAGAA AAGGTTTCGA GCTTCAGCCC
    ACGCTCTATT CAGGGATTAA TCTAGCAGTG CTGCTTATAG TAGCCGGGCA GCAGTTCGAG
    ACTTCCATAG AACTCAGGAA GATCGGTGTA AGGTTGAACA GTCTGCTGGG CCGTAAAGGC
    AGTCTGGAGA AGATGAATAA CTACTGGGAC GTTGGACAGT TTTTTACTGT CAGCATGCTG
    GCCAATGACA TCCCCAAAGC TGTGCAGGCC GCTGAGAAAC TCTTCAAGCT CAAACCACCT
    ATATGGTACC TGCGGTCAGT GGTCCAAAAC CTCCAGCTGA TTCAGCATTT CAAGAAGCAG
    AACACTGAAC ACTCGCCTCA AAGAGAGAGA CTCAACTTCT GGATGGACAT TATCGTGGAG
    GCCACGCAGG GAACCACCAA CGGCCTTCGT TTTCCGGTGT TGATTCTGGA GCCCACTAAA
    GTTTACCAGC CATCTTATGT ATCGATTAAC AGTGAAGCAG AAGAGAAGAA TGTGTCTATA
    TGGCACGTGT CGCCCGCTGA GACGAAGGGA ATACACGAGT GGAACTTCAC TGCGTCGTCC
    ATTAAAGGCA TCAGTATCTC AAAGTTTGAT GAGCGCTGCT GCTTTCTGTA CGTCCATGAT
    AATTCAGATG ACTTCCAGAT CTACTTCTCC ACAGAGGACC AGTGCGGCAG GTTCTGCTCA
    ATGGTGAAAG AGCTCATCAC AGATGGATCT GGAAATGCTG TTGAGCTGGA GGGCGAAGGA
    GACGGAGACA CCTTGGAGTA TGAGTATGAC TACAATGAGA ACGGTGACCG GGTGGTTCTG
    GGCAGGGGGA CCTATGGAGT TGTCTACGCA GGGAGAGACC TGAGCAATCA GGTGCGCATT
    GCCATCAAGG AGATCCCTGA GAGAGACAGC AGGTATTCCC AACCACTGCA TGAGGAAATC
    GCCCTTCACA AGTACCTGAA ACACAGAAAC ATAGTTCAGT ACCTGGGCTC AGTGTCAGAG
    GATGGTTATA TCAAGATCTT CATGGAGCAG GTCCCTGGAG GAAGTCTGTC AGCGTTGTTG
    CGTTCAAAGT GGGGTCCTTT GAAAGAAGCC ACCATAATCT TTTACACTCG GCAGATCCTG
    GAAGGCATTC GTTATTTGCA TGAAAACCAA ATAGTTCATC GGGATATCAA AGGAGACAAT
    GTTTTAGTCA ACACTTACAG TGGCGTGTTG AAAATCTCCG ACTTCGGCAC GTCAAAGAGA
    CTGGCTGGAG TCAATCCCTG CACTGAGACC TTCACTGGCA CCTTGCAGTA TATGGCCCCT
    GAGATCATCG ATAAGGGACC CAGAGGTTAT GGGGCCCCTG CTGACATCTG GTCTTTGGGC
    TGCACCATCA TTGAGATGGC GACAGGCAAA CCTCCTTTTC ATGAGCTGGG AGAGCCACAG
    GCCGCCATGT TTAAGGTGGG CATGTTTAAG ATCCATCCGG AGATCCCGGA GTCTCTTTCG
    TCGGAGGCAA AATCCTTCAT CCTGTCCAGC TTTGAGCCTG ATCCCAATAA ACGAGCAATG
    GCCGGCGACC TGCTCAAAGA CCCCTTCCTG CGGCAGAACA TCAAAGGCAA GAAGAACAAG
    ATCGCTTTCA AACCCTCAGT GTGGCAAGAC TACATCCGCA GTGTTTCTCT ACCCGTCCAG
    CTGCAGACAG AAGCCACAGG CAGCAGCAGC AGCGAGCCGG GCTCCGTCTC TCCAGACTGC
    GACTCCAAAC AAGATGTGTT TTTCGAGAAG AAGAAGCGTT CAAGCTCCGA AAACCTCATT
    AAACCACCCA GCTCAACCTT TCTCAGTGTT CCAGATGAGA GTCCGACAGC AGAAGACCGT
    AGCTCTCCAG CCTCCTCTGA GAACAGCGAC TCTGGTCTCT TCCTGCTCAA GAAAGACAGT
    GAAAGACGGG CCATTCTCTA CAAGGTCCTC AATGAAGACC AGGATAAAGT AACCTCCAAC
    CTCATGGAGA ACCACATCCA GGGTCAAGAG GAGCTGAAGT TGTCTGTGGA TCACATTAAA
    CAAATCATCT GCATCTTGAG AGACTTCATA CGCTGCCCTG AACGTCGTGT TATGGCCACC
    ACCATCTCAA AACTCAAACT GGACCTGGAC TTTGATAGCA CATCGATCAA CCAGATTCAA
    CTCGTTCTGT TCGGCTTTCA GGACTCTGTG AATAAAGTGT TACGGAACCA CCACATTAAA
    CCACACTGGA TGTTTGCCAT GGATAACATC ATCAGACGGG CTGTGCAGGC AGCTGTTACC
    ATTTTAATTC CAGAGCTGCA GACACATTTT GGTCCCGCAT CAGAGAGCGA GGGCGCAGAT
    AAAGACGCAG ATGAAGTAGA TGAAGAGGAC GATGCAGACT TTGGATCAGT AGCACGTACA
    ACACAGGAAG ATCAAGTTCT CACATCTGGA GTCAGCACAC TTAGTTCAGT CATATCCCAT
    GATGCGCAGC GGCTACAGCA CCCCCTAGGA GCACAGCTGG GACGACTCAA ACAGGAAACT
    TGCAGGCTTC TGGAGGAGCT GGTGCAGAAG GAGAGAGAAT ATCAGCAGGT TCTGAGACAA
    ACTCTCCAAC AGCGGGTACA TGACATCGAG CTTATCAGGA TCAGACAGCA GCCTGCTGCA
    GAGATGCCCA CTACACCCTC AGTATTTCAC GTTACTGCAC AACCCGAGCC TGACAAGAAG
    CTGACGGATT GGCTGAAGGA GCAGGGAGCA GACAGCGAGA CGATAGACAA GTTTGTTGCA
    GAAGATTATT CACTCAGCGA CGTTCTCTGC GATATTACTA AAGAAGATCT TCGATGCCTG
    AGGCTTAGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021329473.1
    CDS776..4687
    Translation

    Target ORF information:

    RefSeq Version XM_021473798.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio mitogen-activated protein kinase kinase kinase 15 (map3k15), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021473798.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAGATCA TGTCCAATCT ACCTACAGGA GAGGCTGGTG GCGACCACAC CAATGCGGTT 
    TCCATGTCTG CGGGCATGGA GAGAGCCGAT GTGACCAGCC CGAGTCCGGT GAGCAAACAG
    AGGTCCCTGC GGGCTGTGTA TGTGCTGAAT GATGGACTGA AGGCAGTGGC TGCGAATAGC
    CCGGAGTCCG GGGCTCTCCA GTGTCTCCAG CGCGCCTGCG ACGCGGAGAG TGCCATATTG
    ACCACAGTCA CCTTCGGACG ATTAGACTTT GGGGAGACCA CTGTGCTTGA TACTTTTTAC
    GATGCAGATA TTGCAGTTGT GGACATGAGT GATGTGTTCA GACAGCCCTC TTTGTTTTAT
    CACCTCGGGG TCCGTGAGAG CTTTGACATG GCCAACAATG TTATCCTCTA CCACGACATG
    GACCCCGACA CAGCACAGTC CCTTAAGGAC ATGGTGGCAC AGAAAAACAC AGCCTCTAGT
    GGGAACTACT ATTTCATCCC ATACCTGATG ACACCCAACA ACGAGTACTT GTGCTGTGAG
    AATGTGGCCC AGCGCAGAGC ATCAGAGTAC ATGCAGCCCA ACTGGGACAA CCTTTTAGGA
    CCTCTGTGTG TCCCTTTGGT GGACCGCTTC GTCAGCCTTC TCAAAGACAT TCATGTCACG
    TCTTGTGCTT CATACAAAGA TGCACTCCTG AATGACATCC GAAAAGCTCG AGATAAATAC
    CAGGGTGAGG AGCTGGCCAA AGAGCTTTCC CGTATCAAAC TCCGCATCGA CAACACTGAG
    GTTTTGACTC AAGATATTGT CATGAACCTG CTTTTCTCCT ACAGAGACAT ACAGGATTAT
    GATGCTATGG TTAAACTGGT GCAGACCATT GAGATGTTGC CCACCTGTGA CTTGGCCAAC
    CAGCCCATGA TTCAGTTCCA CTATGCTTTT GCGCTCAATA GACGAAACAG CCCTGGTGAT
    CGAGAGCAGG CGCTGCGTGT GATGTTGCAG GTTCTTCAAT CGTGTGAACA CCCTGCTCCT
    GACATGTTCT GCTTGTGTGG CCGCATATAT AAAGACATCT TTCTAGACTC AGACTGTAAA
    GACACCAAAA ACAGAGACAA CGCCATCCAG TGGTATAGAA AAGGTTTCGA GCTTCAGCCC
    ACGCTCTATT CAGGGATTAA TCTAGCAGTG CTGCTTATAG TAGCCGGGCA GCAGTTCGAG
    ACTTCCATAG AACTCAGGAA GATCGGTGTA AGGTTGAACA GTCTGCTGGG CCGTAAAGGC
    AGTCTGGAGA AGATGAATAA CTACTGGGAC GTTGGACAGT TTTTTACTGT CAGCATGCTG
    GCCAATGACA TCCCCAAAGC TGTGCAGGCC GCTGAGAAAC TCTTCAAGCT CAAACCACCT
    ATATGGTACC TGCGGTCAGT GGTCCAAAAC CTCCAGCTGA TTCAGCATTT CAAGAAGCAG
    AACACTGAAC ACTCGCCTCA AAGAGAGAGA CTCAACTTCT GGATGGACAT TATCGTGGAG
    GCCACGCAGG GAACCACCAA CGGCCTTCGT TTTCCGGTGT TGATTCTGGA GCCCACTAAA
    GTTTACCAGC CATCTTATGT ATCGATTAAC AGTGAAGCAG AAGAGAAGAA TGTGTCTATA
    TGGCACGTGT CGCCCGCTGA GACGAAGGGA ATACACGAGT GGAACTTCAC TGCGTCGTCC
    ATTAAAGGCA TCAGTATCTC AAAGTTTGAT GAGCGCTGCT GCTTTCTGTA CGTCCATGAT
    AATTCAGATG ACTTCCAGAT CTACTTCTCC ACAGAGGACC AGTGCGGCAG GTTCTGCTCA
    ATGGTGAAAG AGCTCATCAC AGATGGATCT GGAAATGCTG TTGAGCTGGA GGGCGAAGGA
    GACGGAGACA CCTTGGAGTA TGAGTATGAC TACAATGAGA ACGGTGACCG GGTGGTTCTG
    GGCAGGGGGA CCTATGGAGT TGTCTACGCA GGGAGAGACC TGAGCAATCA GGTGCGCATT
    GCCATCAAGG AGATCCCTGA GAGAGACAGC AGGTATTCCC AACCACTGCA TGAGGAAATC
    GCCCTTCACA AGTACCTGAA ACACAGAAAC ATAGTTCAGT ACCTGGGCTC AGTGTCAGAG
    GATGGTTATA TCAAGATCTT CATGGAGCAG GTCCCTGGAG GAAGTCTGTC AGCGTTGTTG
    CGTTCAAAGT GGGGTCCTTT GAAAGAAGCC ACCATAATCT TTTACACTCG GCAGATCCTG
    GAAGGCATTC GTTATTTGCA TGAAAACCAA ATAGTTCATC GGGATATCAA AGGAGACAAT
    GTTTTAGTCA ACACTTACAG TGGCGTGTTG AAAATCTCCG ACTTCGGCAC GTCAAAGAGA
    CTGGCTGGAG TCAATCCCTG CACTGAGACC TTCACTGGCA CCTTGCAGTA TATGGCCCCT
    GAGATCATCG ATAAGGGACC CAGAGGTTAT GGGGCCCCTG CTGACATCTG GTCTTTGGGC
    TGCACCATCA TTGAGATGGC GACAGGCAAA CCTCCTTTTC ATGAGCTGGG AGAGCCACAG
    GCCGCCATGT TTAAGGTGGG CATGTTTAAG ATCCATCCGG AGATCCCGGA GTCTCTTTCG
    TCGGAGGCAA AATCCTTCAT CCTGTCCAGC TTTGAGCCTG ATCCCAATAA ACGAGCAATG
    GCCGGCGACC TGCTCAAAGA CCCCTTCCTG CGGCAGAACA TCAAAGGCAA GAAGAACAAG
    ATCGCTTTCA AACCCTCAGT GTGGCAAGAC TACATCCGCA GTGTTTCTCT ACCCGTCCAG
    CTGCAGACAG AAGCCACAGG CAGCAGCAGC AGCGAGCCGG GCTCCGTCTC TCCAGACTGC
    GACTCCAAAC AAGATGTGTT TTTCGAGAAG AAGAAGCGTT CAAGCTCCGA AAACCTCATT
    AAACCACCCA GCTCAACCTT TCTCAGTGTT CCAGATGAGA GTCCGACAGC AGAAGACCGT
    AGCTCTCCAG CCTCCTCTGA GAACAGCGAC TCTGGTCTCT TCCTGCTCAA GAAAGACAGT
    GAAAGACGGG CCATTCTCTA CAAGGTCCTC AATGAAGACC AGGATAAAGT AACCTCCAAC
    CTCATGGAGA ACCACATCCA GGGTCAAGAG GAGCTGAAGT TGTCTGTGGA TCACATTAAA
    CAAATCATCT GCATCTTGAG AGACTTCATA CGCTGCCCTG AACGTCGTGT TATGGCCACC
    ACCATCTCAA AACTCAAACT GGACCTGGAC TTTGATAGCA CATCGATCAA CCAGATTCAA
    CTCGTTCTGT TCGGCTTTCA GGACTCTGTG AATAAAGTGT TACGGAACCA CCACATTAAA
    CCACACTGGA TGTTTGCCAT GGATAACATC ATCAGACGGG CTGTGCAGGC AGCTGTTACC
    ATTTTAATTC CAGAGCTGCA GACACATTTT GGTCCCGCAT CAGAGAGCGA GGGCGCAGAT
    AAAGACGCAG ATGAAGTAGA TGAAGAGGAC GATGCAGACT TTGGATCAGT AGCACGTACA
    ACACAGGAAG ATCAAGTTCT CACATCTGGA GTCAGCACAC TTAGTTCAGT CATATCCCAT
    GATGCGCAGC GGCTACAGCA CCCCCTAGGA GCACAGCTGG GACGACTCAA ACAGGAAACT
    TGCAGGCTTC TGGAGGAGCT GGTGCAGAAG GAGAGAGAAT ATCAGCAGGT TCTGAGACAA
    ACTCTCCAAC AGCGGGTACA TGACATCGAG CTTATCAGGA TCAGACAGCA GCCTGCTGCA
    GAGATGCCCA CTACACCCTC AGTATTTCAC GTTACTGCAC AACCCGAGCC TGACAAGAAG
    CTGACGGATT GGCTGAAGGA GCAGGGAGCA GACAGCGAGA CGATAGACAA GTTTGTTGCA
    GAAGATTATT CACTCAGCGA CGTTCTCTGC GATATTACTA AAGAAGATCT TCGATGCCTG
    AGGCTTAGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa141227
    Clone ID Related Accession (Same CDS sequence) XM_021473798.1 , XM_021470343.1
    Accession Version XM_021470343.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3912bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product mitogen-activated protein kinase kinase kinase 15 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAGATCA TGTCCAATCT ACCTACAGGA GAGGCTGGTG GCGACCACAC CAATGCGGTT 
    TCCATGTCTG CGGGCATGGA GAGAGCCGAT GTGACCAGCC CGAGTCCGGT GAGCAAACAG
    AGGTCCCTGC GGGCTGTGTA TGTGCTGAAT GATGGACTGA AGGCAGTGGC TGCGAATAGC
    CCGGAGTCCG GGGCTCTCCA GTGTCTCCAG CGCGCCTGCG ACGCGGAGAG TGCCATATTG
    ACCACAGTCA CCTTCGGACG ATTAGACTTT GGGGAGACCA CTGTGCTTGA TACTTTTTAC
    GATGCAGATA TTGCAGTTGT GGACATGAGT GATGTGTTCA GACAGCCCTC TTTGTTTTAT
    CACCTCGGGG TCCGTGAGAG CTTTGACATG GCCAACAATG TTATCCTCTA CCACGACATG
    GACCCCGACA CAGCACAGTC CCTTAAGGAC ATGGTGGCAC AGAAAAACAC AGCCTCTAGT
    GGGAACTACT ATTTCATCCC ATACCTGATG ACACCCAACA ACGAGTACTT GTGCTGTGAG
    AATGTGGCCC AGCGCAGAGC ATCAGAGTAC ATGCAGCCCA ACTGGGACAA CCTTTTAGGA
    CCTCTGTGTG TCCCTTTGGT GGACCGCTTC GTCAGCCTTC TCAAAGACAT TCATGTCACG
    TCTTGTGCTT CATACAAAGA TGCACTCCTG AATGACATCC GAAAAGCTCG AGATAAATAC
    CAGGGTGAGG AGCTGGCCAA AGAGCTTTCC CGTATCAAAC TCCGCATCGA CAACACTGAG
    GTTTTGACTC AAGATATTGT CATGAACCTG CTTTTCTCCT ACAGAGACAT ACAGGATTAT
    GATGCTATGG TTAAACTGGT GCAGACCATT GAGATGTTGC CCACCTGTGA CTTGGCCAAC
    CAGCCCATGA TTCAGTTCCA CTATGCTTTT GCGCTCAATA GACGAAACAG CCCTGGTGAT
    CGAGAGCAGG CGCTGCGTGT GATGTTGCAG GTTCTTCAAT CGTGTGAACA CCCTGCTCCT
    GACATGTTCT GCTTGTGTGG CCGCATATAT AAAGACATCT TTCTAGACTC AGACTGTAAA
    GACACCAAAA ACAGAGACAA CGCCATCCAG TGGTATAGAA AAGGTTTCGA GCTTCAGCCC
    ACGCTCTATT CAGGGATTAA TCTAGCAGTG CTGCTTATAG TAGCCGGGCA GCAGTTCGAG
    ACTTCCATAG AACTCAGGAA GATCGGTGTA AGGTTGAACA GTCTGCTGGG CCGTAAAGGC
    AGTCTGGAGA AGATGAATAA CTACTGGGAC GTTGGACAGT TTTTTACTGT CAGCATGCTG
    GCCAATGACA TCCCCAAAGC TGTGCAGGCC GCTGAGAAAC TCTTCAAGCT CAAACCACCT
    ATATGGTACC TGCGGTCAGT GGTCCAAAAC CTCCAGCTGA TTCAGCATTT CAAGAAGCAG
    AACACTGAAC ACTCGCCTCA AAGAGAGAGA CTCAACTTCT GGATGGACAT TATCGTGGAG
    GCCACGCAGG GAACCACCAA CGGCCTTCGT TTTCCGGTGT TGATTCTGGA GCCCACTAAA
    GTTTACCAGC CATCTTATGT ATCGATTAAC AGTGAAGCAG AAGAGAAGAA TGTGTCTATA
    TGGCACGTGT CGCCCGCTGA GACGAAGGGA ATACACGAGT GGAACTTCAC TGCGTCGTCC
    ATTAAAGGCA TCAGTATCTC AAAGTTTGAT GAGCGCTGCT GCTTTCTGTA CGTCCATGAT
    AATTCAGATG ACTTCCAGAT CTACTTCTCC ACAGAGGACC AGTGCGGCAG GTTCTGCTCA
    ATGGTGAAAG AGCTCATCAC AGATGGATCT GGAAATGCTG TTGAGCTGGA GGGCGAAGGA
    GACGGAGACA CCTTGGAGTA TGAGTATGAC TACAATGAGA ACGGTGACCG GGTGGTTCTG
    GGCAGGGGGA CCTATGGAGT TGTCTACGCA GGGAGAGACC TGAGCAATCA GGTGCGCATT
    GCCATCAAGG AGATCCCTGA GAGAGACAGC AGGTATTCCC AACCACTGCA TGAGGAAATC
    GCCCTTCACA AGTACCTGAA ACACAGAAAC ATAGTTCAGT ACCTGGGCTC AGTGTCAGAG
    GATGGTTATA TCAAGATCTT CATGGAGCAG GTCCCTGGAG GAAGTCTGTC AGCGTTGTTG
    CGTTCAAAGT GGGGTCCTTT GAAAGAAGCC ACCATAATCT TTTACACTCG GCAGATCCTG
    GAAGGCATTC GTTATTTGCA TGAAAACCAA ATAGTTCATC GGGATATCAA AGGAGACAAT
    GTTTTAGTCA ACACTTACAG TGGCGTGTTG AAAATCTCCG ACTTCGGCAC GTCAAAGAGA
    CTGGCTGGAG TCAATCCCTG CACTGAGACC TTCACTGGCA CCTTGCAGTA TATGGCCCCT
    GAGATCATCG ATAAGGGACC CAGAGGTTAT GGGGCCCCTG CTGACATCTG GTCTTTGGGC
    TGCACCATCA TTGAGATGGC GACAGGCAAA CCTCCTTTTC ATGAGCTGGG AGAGCCACAG
    GCCGCCATGT TTAAGGTGGG CATGTTTAAG ATCCATCCGG AGATCCCGGA GTCTCTTTCG
    TCGGAGGCAA AATCCTTCAT CCTGTCCAGC TTTGAGCCTG ATCCCAATAA ACGAGCAATG
    GCCGGCGACC TGCTCAAAGA CCCCTTCCTG CGGCAGAACA TCAAAGGCAA GAAGAACAAG
    ATCGCTTTCA AACCCTCAGT GTGGCAAGAC TACATCCGCA GTGTTTCTCT ACCCGTCCAG
    CTGCAGACAG AAGCCACAGG CAGCAGCAGC AGCGAGCCGG GCTCCGTCTC TCCAGACTGC
    GACTCCAAAC AAGATGTGTT TTTCGAGAAG AAGAAGCGTT CAAGCTCCGA AAACCTCATT
    AAACCACCCA GCTCAACCTT TCTCAGTGTT CCAGATGAGA GTCCGACAGC AGAAGACCGT
    AGCTCTCCAG CCTCCTCTGA GAACAGCGAC TCTGGTCTCT TCCTGCTCAA GAAAGACAGT
    GAAAGACGGG CCATTCTCTA CAAGGTCCTC AATGAAGACC AGGATAAAGT AACCTCCAAC
    CTCATGGAGA ACCACATCCA GGGTCAAGAG GAGCTGAAGT TGTCTGTGGA TCACATTAAA
    CAAATCATCT GCATCTTGAG AGACTTCATA CGCTGCCCTG AACGTCGTGT TATGGCCACC
    ACCATCTCAA AACTCAAACT GGACCTGGAC TTTGATAGCA CATCGATCAA CCAGATTCAA
    CTCGTTCTGT TCGGCTTTCA GGACTCTGTG AATAAAGTGT TACGGAACCA CCACATTAAA
    CCACACTGGA TGTTTGCCAT GGATAACATC ATCAGACGGG CTGTGCAGGC AGCTGTTACC
    ATTTTAATTC CAGAGCTGCA GACACATTTT GGTCCCGCAT CAGAGAGCGA GGGCGCAGAT
    AAAGACGCAG ATGAAGTAGA TGAAGAGGAC GATGCAGACT TTGGATCAGT AGCACGTACA
    ACACAGGAAG ATCAAGTTCT CACATCTGGA GTCAGCACAC TTAGTTCAGT CATATCCCAT
    GATGCGCAGC GGCTACAGCA CCCCCTAGGA GCACAGCTGG GACGACTCAA ACAGGAAACT
    TGCAGGCTTC TGGAGGAGCT GGTGCAGAAG GAGAGAGAAT ATCAGCAGGT TCTGAGACAA
    ACTCTCCAAC AGCGGGTACA TGACATCGAG CTTATCAGGA TCAGACAGCA GCCTGCTGCA
    GAGATGCCCA CTACACCCTC AGTATTTCAC GTTACTGCAC AACCCGAGCC TGACAAGAAG
    CTGACGGATT GGCTGAAGGA GCAGGGAGCA GACAGCGAGA CGATAGACAA GTTTGTTGCA
    GAAGATTATT CACTCAGCGA CGTTCTCTGC GATATTACTA AAGAAGATCT TCGATGCCTG
    AGGCTTAGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326018.1
    CDS776..4687
    Translation

    Target ORF information:

    RefSeq Version XM_021470343.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio mitogen-activated protein kinase kinase kinase 15 (map3k15), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021470343.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAGATCA TGTCCAATCT ACCTACAGGA GAGGCTGGTG GCGACCACAC CAATGCGGTT 
    TCCATGTCTG CGGGCATGGA GAGAGCCGAT GTGACCAGCC CGAGTCCGGT GAGCAAACAG
    AGGTCCCTGC GGGCTGTGTA TGTGCTGAAT GATGGACTGA AGGCAGTGGC TGCGAATAGC
    CCGGAGTCCG GGGCTCTCCA GTGTCTCCAG CGCGCCTGCG ACGCGGAGAG TGCCATATTG
    ACCACAGTCA CCTTCGGACG ATTAGACTTT GGGGAGACCA CTGTGCTTGA TACTTTTTAC
    GATGCAGATA TTGCAGTTGT GGACATGAGT GATGTGTTCA GACAGCCCTC TTTGTTTTAT
    CACCTCGGGG TCCGTGAGAG CTTTGACATG GCCAACAATG TTATCCTCTA CCACGACATG
    GACCCCGACA CAGCACAGTC CCTTAAGGAC ATGGTGGCAC AGAAAAACAC AGCCTCTAGT
    GGGAACTACT ATTTCATCCC ATACCTGATG ACACCCAACA ACGAGTACTT GTGCTGTGAG
    AATGTGGCCC AGCGCAGAGC ATCAGAGTAC ATGCAGCCCA ACTGGGACAA CCTTTTAGGA
    CCTCTGTGTG TCCCTTTGGT GGACCGCTTC GTCAGCCTTC TCAAAGACAT TCATGTCACG
    TCTTGTGCTT CATACAAAGA TGCACTCCTG AATGACATCC GAAAAGCTCG AGATAAATAC
    CAGGGTGAGG AGCTGGCCAA AGAGCTTTCC CGTATCAAAC TCCGCATCGA CAACACTGAG
    GTTTTGACTC AAGATATTGT CATGAACCTG CTTTTCTCCT ACAGAGACAT ACAGGATTAT
    GATGCTATGG TTAAACTGGT GCAGACCATT GAGATGTTGC CCACCTGTGA CTTGGCCAAC
    CAGCCCATGA TTCAGTTCCA CTATGCTTTT GCGCTCAATA GACGAAACAG CCCTGGTGAT
    CGAGAGCAGG CGCTGCGTGT GATGTTGCAG GTTCTTCAAT CGTGTGAACA CCCTGCTCCT
    GACATGTTCT GCTTGTGTGG CCGCATATAT AAAGACATCT TTCTAGACTC AGACTGTAAA
    GACACCAAAA ACAGAGACAA CGCCATCCAG TGGTATAGAA AAGGTTTCGA GCTTCAGCCC
    ACGCTCTATT CAGGGATTAA TCTAGCAGTG CTGCTTATAG TAGCCGGGCA GCAGTTCGAG
    ACTTCCATAG AACTCAGGAA GATCGGTGTA AGGTTGAACA GTCTGCTGGG CCGTAAAGGC
    AGTCTGGAGA AGATGAATAA CTACTGGGAC GTTGGACAGT TTTTTACTGT CAGCATGCTG
    GCCAATGACA TCCCCAAAGC TGTGCAGGCC GCTGAGAAAC TCTTCAAGCT CAAACCACCT
    ATATGGTACC TGCGGTCAGT GGTCCAAAAC CTCCAGCTGA TTCAGCATTT CAAGAAGCAG
    AACACTGAAC ACTCGCCTCA AAGAGAGAGA CTCAACTTCT GGATGGACAT TATCGTGGAG
    GCCACGCAGG GAACCACCAA CGGCCTTCGT TTTCCGGTGT TGATTCTGGA GCCCACTAAA
    GTTTACCAGC CATCTTATGT ATCGATTAAC AGTGAAGCAG AAGAGAAGAA TGTGTCTATA
    TGGCACGTGT CGCCCGCTGA GACGAAGGGA ATACACGAGT GGAACTTCAC TGCGTCGTCC
    ATTAAAGGCA TCAGTATCTC AAAGTTTGAT GAGCGCTGCT GCTTTCTGTA CGTCCATGAT
    AATTCAGATG ACTTCCAGAT CTACTTCTCC ACAGAGGACC AGTGCGGCAG GTTCTGCTCA
    ATGGTGAAAG AGCTCATCAC AGATGGATCT GGAAATGCTG TTGAGCTGGA GGGCGAAGGA
    GACGGAGACA CCTTGGAGTA TGAGTATGAC TACAATGAGA ACGGTGACCG GGTGGTTCTG
    GGCAGGGGGA CCTATGGAGT TGTCTACGCA GGGAGAGACC TGAGCAATCA GGTGCGCATT
    GCCATCAAGG AGATCCCTGA GAGAGACAGC AGGTATTCCC AACCACTGCA TGAGGAAATC
    GCCCTTCACA AGTACCTGAA ACACAGAAAC ATAGTTCAGT ACCTGGGCTC AGTGTCAGAG
    GATGGTTATA TCAAGATCTT CATGGAGCAG GTCCCTGGAG GAAGTCTGTC AGCGTTGTTG
    CGTTCAAAGT GGGGTCCTTT GAAAGAAGCC ACCATAATCT TTTACACTCG GCAGATCCTG
    GAAGGCATTC GTTATTTGCA TGAAAACCAA ATAGTTCATC GGGATATCAA AGGAGACAAT
    GTTTTAGTCA ACACTTACAG TGGCGTGTTG AAAATCTCCG ACTTCGGCAC GTCAAAGAGA
    CTGGCTGGAG TCAATCCCTG CACTGAGACC TTCACTGGCA CCTTGCAGTA TATGGCCCCT
    GAGATCATCG ATAAGGGACC CAGAGGTTAT GGGGCCCCTG CTGACATCTG GTCTTTGGGC
    TGCACCATCA TTGAGATGGC GACAGGCAAA CCTCCTTTTC ATGAGCTGGG AGAGCCACAG
    GCCGCCATGT TTAAGGTGGG CATGTTTAAG ATCCATCCGG AGATCCCGGA GTCTCTTTCG
    TCGGAGGCAA AATCCTTCAT CCTGTCCAGC TTTGAGCCTG ATCCCAATAA ACGAGCAATG
    GCCGGCGACC TGCTCAAAGA CCCCTTCCTG CGGCAGAACA TCAAAGGCAA GAAGAACAAG
    ATCGCTTTCA AACCCTCAGT GTGGCAAGAC TACATCCGCA GTGTTTCTCT ACCCGTCCAG
    CTGCAGACAG AAGCCACAGG CAGCAGCAGC AGCGAGCCGG GCTCCGTCTC TCCAGACTGC
    GACTCCAAAC AAGATGTGTT TTTCGAGAAG AAGAAGCGTT CAAGCTCCGA AAACCTCATT
    AAACCACCCA GCTCAACCTT TCTCAGTGTT CCAGATGAGA GTCCGACAGC AGAAGACCGT
    AGCTCTCCAG CCTCCTCTGA GAACAGCGAC TCTGGTCTCT TCCTGCTCAA GAAAGACAGT
    GAAAGACGGG CCATTCTCTA CAAGGTCCTC AATGAAGACC AGGATAAAGT AACCTCCAAC
    CTCATGGAGA ACCACATCCA GGGTCAAGAG GAGCTGAAGT TGTCTGTGGA TCACATTAAA
    CAAATCATCT GCATCTTGAG AGACTTCATA CGCTGCCCTG AACGTCGTGT TATGGCCACC
    ACCATCTCAA AACTCAAACT GGACCTGGAC TTTGATAGCA CATCGATCAA CCAGATTCAA
    CTCGTTCTGT TCGGCTTTCA GGACTCTGTG AATAAAGTGT TACGGAACCA CCACATTAAA
    CCACACTGGA TGTTTGCCAT GGATAACATC ATCAGACGGG CTGTGCAGGC AGCTGTTACC
    ATTTTAATTC CAGAGCTGCA GACACATTTT GGTCCCGCAT CAGAGAGCGA GGGCGCAGAT
    AAAGACGCAG ATGAAGTAGA TGAAGAGGAC GATGCAGACT TTGGATCAGT AGCACGTACA
    ACACAGGAAG ATCAAGTTCT CACATCTGGA GTCAGCACAC TTAGTTCAGT CATATCCCAT
    GATGCGCAGC GGCTACAGCA CCCCCTAGGA GCACAGCTGG GACGACTCAA ACAGGAAACT
    TGCAGGCTTC TGGAGGAGCT GGTGCAGAAG GAGAGAGAAT ATCAGCAGGT TCTGAGACAA
    ACTCTCCAAC AGCGGGTACA TGACATCGAG CTTATCAGGA TCAGACAGCA GCCTGCTGCA
    GAGATGCCCA CTACACCCTC AGTATTTCAC GTTACTGCAC AACCCGAGCC TGACAAGAAG
    CTGACGGATT GGCTGAAGGA GCAGGGAGCA GACAGCGAGA CGATAGACAA GTTTGTTGCA
    GAAGATTATT CACTCAGCGA CGTTCTCTGC GATATTACTA AAGAAGATCT TCGATGCCTG
    AGGCTTAGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa28664
    Clone ID Related Accession (Same CDS sequence) XM_021473796.1 , XM_009297482.2 , XM_009297482.3
    Accession Version XM_021473796.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3999bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product mitogen-activated protein kinase kinase kinase 15 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395177.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGATCA TGTCCAATCT ACCTACAGGA GAGGCTGGTG GCGACCACAC CAATGCGGTT 
    TCCATGTCTG CGGGCATGGA GAGAGCCGAT GTGACCAGCC CGAGTCCGGT GAGCAAACAG
    AGGTCCCTGC GGGCTGTGTA TGTGCTGAAT GATGGACTGA AGGCAGTGGC TGCGAATAGC
    CCGGAGTCCG GGGCTCTCCA GTGTCTCCAG CGCGCCTGCG ACGCGGAGAG TGCCATATTG
    ACCACAGTCA CCTTCGGACG ATTAGACTTT GGGGAGACCA CTGTGCTTGA TACTTTTTAC
    GATGCAGATA TTGCAGTTGT GGACATGAGT GATGTGTTCA GACAGCCCTC TTTGTTTTAT
    CACCTCGGGG TCCGTGAGAG CTTTGACATG GCCAACAATG TTATCCTCTA CCACGACATG
    GACCCCGACA CAGCACAGTC CCTTAAGGAC ATGGTGGCAC AGAAAAACAC AGCCTCTAGT
    GGGAACTACT ATTTCATCCC ATACCTGATG ACACCCAACA ACGAGTACTT GTGCTGTGAG
    AATGTGGCCC AGCGCAGAGC ATCAGAGTAC ATGCAGCCCA ACTGGGACAA CCTTTTAGGA
    CCTCTGTGTG TCCCTTTGGT GGACCGCTTC GTCAGCCTTC TCAAAGACAT TCATGTCACG
    TCTTGTGCTT CATACAAAGA TGCACTCCTG AATGACATCC GAAAAGCTCG AGATAAATAC
    CAGGGTGAGG AGCTGGCCAA AGAGCTTTCC CGTATCAAAC TCCGCATCGA CAACACTGAG
    GTTTTGACTC AAGATATTGT CATGAACCTG CTTTTCTCCT ACAGAGACAT ACAGGATTAT
    GATGCTATGG TTAAACTGGT GCAGACCATT GAGATGTTGC CCACCTGTGA CTTGGCCAAC
    CAGCCCATGA TTCAGTTCCA CTATGCTTTT GCGCTCAATA GACGAAACAG CCCTGGTGAT
    CGAGAGCAGG CGCTGCGTGT GATGTTGCAG GTTCTTCAAT CGTGTGAACA CCCTGCTCCT
    GACATGTTCT GCTTGTGTGG CCGCATATAT AAAGACATCT TTCTAGACTC AGACTGTAAA
    GACACCAAAA ACAGAGACAA CGCCATCCAG TGGTATAGAA AAGGTTTCGA GCTTCAGCCC
    ACGCTCTATT CAGGGATTAA TCTAGCAGTG CTGCTTATAG TAGCCGGGCA GCAGTTCGAG
    ACTTCCATAG AACTCAGGAA GATCGGTGTA AGGTTGAACA GTCTGCTGGG CCGTAAAGGC
    AGTCTGGAGA AGATGAATAA CTACTGGGAC GTTGGACAGT TTTTTACTGT CAGCATGCTG
    GCCAATGACA TCCCCAAAGC TGTGCAGGCC GCTGAGAAAC TCTTCAAGCT CAAACCACCT
    ATATGGTACC TGCGGTCAGT GGTCCAAAAC CTCCAGCTGA TTCAGCATTT CAAGAAGCAG
    AACACTGAAC ACTCGCCTCA AAGAGAGAGA CTCAACTTCT GGATGGACAT TATCGTGGAG
    GCCACGCAGG GAACCACCAA CGGCCTTCGT TTTCCGGTGT TGATTCTGGA GCCCACTAAA
    GTTTACCAGC CATCTTATGT ATCGATTAAC AGTGAAGCAG AAGAGAAGAA TGTGTCTATA
    TGGCACGTGT CGCCCGCTGA GACGAAGGGA ATACACGAGT GGAACTTCAC TGCGTCGTCC
    ATTAAAGGCA TCAGTATCTC AAAGTTTGAT GAGCGCTGCT GCTTTCTGTA CGTCCATGAT
    AATTCAGATG ACTTCCAGAT CTACTTCTCC ACAGAGGACC AGTGCGGCAG GTTCTGCTCA
    ATGGTGAAAG AGCTCATCAC AGATGGATCT GGAAATGCTG TTGAGCTGGA GGGCGAAGGA
    GACGGAGACA CCTTGGAGTA TGAGTATGAC TACAATGAGA ACGGTGACCG GGTGGTTCTG
    GGCAGGGGGA CCTATGGAGT TGTCTACGCA GGGAGAGACC TGAGCAATCA GGTGCGCATT
    GCCATCAAGG AGATCCCTGA GAGAGACAGC AGGTATTCCC AACCACTGCA TGAGGAAATC
    GCCCTTCACA AGTACCTGAA ACACAGAAAC ATAGTTCAGT ACCTGGGCTC AGTGTCAGAG
    GATGGTTATA TCAAGATCTT CATGGAGCAG GTCCCTGGAG GAAGTCTGTC AGCGTTGTTG
    CGTTCAAAGT GGGGTCCTTT GAAAGAAGCC ACCATAATCT TTTACACTCG GCAGATCCTG
    GAAGGCATTC GTTATTTGCA TGAAAACCAA ATAGTTCATC GGGATATCAA AGGAGACAAT
    GTTTTAGTCA ACACTTACAG TGGCGTGTTG AAAATCTCCG ACTTCGGCAC GTCAAAGAGA
    CTGGCTGGAG TCAATCCCTG CACTGAGACC TTCACTGGCA CCTTGCAGTA TATGGCCCCT
    GAGATCATCG ATAAGGGACC CAGAGGTTAT GGGGCCCCTG CTGACATCTG GTCTTTGGGC
    TGCACCATCA TTGAGATGGC GACAGGCAAA CCTCCTTTTC ATGAGCTGGG AGAGCCACAG
    GCCGCCATGT TTAAGGTGGG CATGTTTAAG ATCCATCCGG AGATCCCGGA GTCTCTTTCG
    TCGGAGGCAA AATCCTTCAT CCTGTCCAGC TTTGAGCCTG ATCCCAATAA ACGAGCAATG
    GCCGGCGACC TGCTCAAAGA CCCCTTCCTG CGGCAGAACA TCAAAGGCAA GAAGAACAAG
    ATCGCTTTCA AACCCTCAGA CTACATCCGC AGTGTTTCTC TACCCGTCCA GCTGCAGACA
    GAAGCCACAG GCAGCAGCAG CAGCGAGCCG GGCTCCGTCT CTCCAGACTG CGACTCCAAA
    CAAGATGTGT TTTTCGAGAA GAAGAAGCGT TCAAGCTCCG AAAACCTCAT TAAACCACCC
    AGCTCAACCT TTCTCAGTGT TCCAGATGAG AGTCCGACAG CAGAAGACCG TAGCTCTCCA
    GCCTCCTCTG AGAACAGCGA CTCTGGTCTC TTCCTGCTCA AGAAAGACAG TGAAAGACGG
    GCCATTCTCT ACAAGGTCCT CAATGAAGAC CAGGATAAAG TAACCTCCAA CCTCATGGAG
    AACCACATCC AGGGTCAAGA GGAGCTGAAG TTGTCTGTGG ATCACATTAA ACAAATCATC
    TGCATCTTGA GAGACTTCAT ACGCTGCCCT GAACGTCGTG TTATGGCCAC CACCATCTCA
    AAACTCAAAC TGGACCTGGA CTTTGATAGC ACATCGATCA ACCAGATTCA ACTCGTTCTG
    TTCGGCTTTC AGGACTCTGT GAATAAAGTG TTACGGAACC ACCACATTAA ACCACACTGG
    ATGTTTGCCA TGGATAACAT CATCAGACGG GCTGTGCAGG CAGCTGTTAC CATTTTAATT
    CCAGAGCTGC AGACACATTT TGGTCCCGCA TCAGAGAGCG AGGGCGCAGA TAAAGACGCA
    GATGAAGTAG ATGAAGAGGA CGATGCAGAC TTTGGATCAG TAGCACGTAC AACACAGGAA
    GATCAAGTTC TCACATCTGG AGTCAGCACA CTTAGTTCAG TCATATCCCA TGATGCGCAG
    CGGCTACAGC ACCCCCTAGG AGCACAGCTG GGACGACTCA AACAGGAAAC TTGCAGGCTT
    CTGGAGGAGC TGGTGCAGAA GGAGAGAGAA TATCAGCAGG TTCTGAGACA AACTCTCCAA
    CAGCGGGTAC ATGACATCGA GCTTATCAGG ATCAGACAGC AGCCTGCTGC AGCAGAGATG
    CCCACTACAC CCTCAGTATT TCACGTTACT GCACAACCCG AGCCTGACAA GAAGCTGACG
    GATTGGCTGA AGGAGCAGGG AGCAGACAGC GAGACGATAG ACAAGTTTGT TGCAGAAGAT
    TATTCACTCA GCGACGTTCT CTGCGATATT ACTAAAGAAG ATCTTCGATG CCTGAGGCTT
    AGGGGTGGAA TGCTGTGTCG GATCTGGAAA GCCATTCAAC AACATCGCAA CAAAGGACTT
    CGAAAGGTCA AAAACGACGA GGGAAATTCA GAAGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021329471.1
    CDS774..4772
    Translation

    Target ORF information:

    RefSeq Version XM_021473796.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio mitogen-activated protein kinase kinase kinase 15 (map3k15), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021473796.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGATCA TGTCCAATCT ACCTACAGGA GAGGCTGGTG GCGACCACAC CAATGCGGTT 
    TCCATGTCTG CGGGCATGGA GAGAGCCGAT GTGACCAGCC CGAGTCCGGT GAGCAAACAG
    AGGTCCCTGC GGGCTGTGTA TGTGCTGAAT GATGGACTGA AGGCAGTGGC TGCGAATAGC
    CCGGAGTCCG GGGCTCTCCA GTGTCTCCAG CGCGCCTGCG ACGCGGAGAG TGCCATATTG
    ACCACAGTCA CCTTCGGACG ATTAGACTTT GGGGAGACCA CTGTGCTTGA TACTTTTTAC
    GATGCAGATA TTGCAGTTGT GGACATGAGT GATGTGTTCA GACAGCCCTC TTTGTTTTAT
    CACCTCGGGG TCCGTGAGAG CTTTGACATG GCCAACAATG TTATCCTCTA CCACGACATG
    GACCCCGACA CAGCACAGTC CCTTAAGGAC ATGGTGGCAC AGAAAAACAC AGCCTCTAGT
    GGGAACTACT ATTTCATCCC ATACCTGATG ACACCCAACA ACGAGTACTT GTGCTGTGAG
    AATGTGGCCC AGCGCAGAGC ATCAGAGTAC ATGCAGCCCA ACTGGGACAA CCTTTTAGGA
    CCTCTGTGTG TCCCTTTGGT GGACCGCTTC GTCAGCCTTC TCAAAGACAT TCATGTCACG
    TCTTGTGCTT CATACAAAGA TGCACTCCTG AATGACATCC GAAAAGCTCG AGATAAATAC
    CAGGGTGAGG AGCTGGCCAA AGAGCTTTCC CGTATCAAAC TCCGCATCGA CAACACTGAG
    GTTTTGACTC AAGATATTGT CATGAACCTG CTTTTCTCCT ACAGAGACAT ACAGGATTAT
    GATGCTATGG TTAAACTGGT GCAGACCATT GAGATGTTGC CCACCTGTGA CTTGGCCAAC
    CAGCCCATGA TTCAGTTCCA CTATGCTTTT GCGCTCAATA GACGAAACAG CCCTGGTGAT
    CGAGAGCAGG CGCTGCGTGT GATGTTGCAG GTTCTTCAAT CGTGTGAACA CCCTGCTCCT
    GACATGTTCT GCTTGTGTGG CCGCATATAT AAAGACATCT TTCTAGACTC AGACTGTAAA
    GACACCAAAA ACAGAGACAA CGCCATCCAG TGGTATAGAA AAGGTTTCGA GCTTCAGCCC
    ACGCTCTATT CAGGGATTAA TCTAGCAGTG CTGCTTATAG TAGCCGGGCA GCAGTTCGAG
    ACTTCCATAG AACTCAGGAA GATCGGTGTA AGGTTGAACA GTCTGCTGGG CCGTAAAGGC
    AGTCTGGAGA AGATGAATAA CTACTGGGAC GTTGGACAGT TTTTTACTGT CAGCATGCTG
    GCCAATGACA TCCCCAAAGC TGTGCAGGCC GCTGAGAAAC TCTTCAAGCT CAAACCACCT
    ATATGGTACC TGCGGTCAGT GGTCCAAAAC CTCCAGCTGA TTCAGCATTT CAAGAAGCAG
    AACACTGAAC ACTCGCCTCA AAGAGAGAGA CTCAACTTCT GGATGGACAT TATCGTGGAG
    GCCACGCAGG GAACCACCAA CGGCCTTCGT TTTCCGGTGT TGATTCTGGA GCCCACTAAA
    GTTTACCAGC CATCTTATGT ATCGATTAAC AGTGAAGCAG AAGAGAAGAA TGTGTCTATA
    TGGCACGTGT CGCCCGCTGA GACGAAGGGA ATACACGAGT GGAACTTCAC TGCGTCGTCC
    ATTAAAGGCA TCAGTATCTC AAAGTTTGAT GAGCGCTGCT GCTTTCTGTA CGTCCATGAT
    AATTCAGATG ACTTCCAGAT CTACTTCTCC ACAGAGGACC AGTGCGGCAG GTTCTGCTCA
    ATGGTGAAAG AGCTCATCAC AGATGGATCT GGAAATGCTG TTGAGCTGGA GGGCGAAGGA
    GACGGAGACA CCTTGGAGTA TGAGTATGAC TACAATGAGA ACGGTGACCG GGTGGTTCTG
    GGCAGGGGGA CCTATGGAGT TGTCTACGCA GGGAGAGACC TGAGCAATCA GGTGCGCATT
    GCCATCAAGG AGATCCCTGA GAGAGACAGC AGGTATTCCC AACCACTGCA TGAGGAAATC
    GCCCTTCACA AGTACCTGAA ACACAGAAAC ATAGTTCAGT ACCTGGGCTC AGTGTCAGAG
    GATGGTTATA TCAAGATCTT CATGGAGCAG GTCCCTGGAG GAAGTCTGTC AGCGTTGTTG
    CGTTCAAAGT GGGGTCCTTT GAAAGAAGCC ACCATAATCT TTTACACTCG GCAGATCCTG
    GAAGGCATTC GTTATTTGCA TGAAAACCAA ATAGTTCATC GGGATATCAA AGGAGACAAT
    GTTTTAGTCA ACACTTACAG TGGCGTGTTG AAAATCTCCG ACTTCGGCAC GTCAAAGAGA
    CTGGCTGGAG TCAATCCCTG CACTGAGACC TTCACTGGCA CCTTGCAGTA TATGGCCCCT
    GAGATCATCG ATAAGGGACC CAGAGGTTAT GGGGCCCCTG CTGACATCTG GTCTTTGGGC
    TGCACCATCA TTGAGATGGC GACAGGCAAA CCTCCTTTTC ATGAGCTGGG AGAGCCACAG
    GCCGCCATGT TTAAGGTGGG CATGTTTAAG ATCCATCCGG AGATCCCGGA GTCTCTTTCG
    TCGGAGGCAA AATCCTTCAT CCTGTCCAGC TTTGAGCCTG ATCCCAATAA ACGAGCAATG
    GCCGGCGACC TGCTCAAAGA CCCCTTCCTG CGGCAGAACA TCAAAGGCAA GAAGAACAAG
    ATCGCTTTCA AACCCTCAGA CTACATCCGC AGTGTTTCTC TACCCGTCCA GCTGCAGACA
    GAAGCCACAG GCAGCAGCAG CAGCGAGCCG GGCTCCGTCT CTCCAGACTG CGACTCCAAA
    CAAGATGTGT TTTTCGAGAA GAAGAAGCGT TCAAGCTCCG AAAACCTCAT TAAACCACCC
    AGCTCAACCT TTCTCAGTGT TCCAGATGAG AGTCCGACAG CAGAAGACCG TAGCTCTCCA
    GCCTCCTCTG AGAACAGCGA CTCTGGTCTC TTCCTGCTCA AGAAAGACAG TGAAAGACGG
    GCCATTCTCT ACAAGGTCCT CAATGAAGAC CAGGATAAAG TAACCTCCAA CCTCATGGAG
    AACCACATCC AGGGTCAAGA GGAGCTGAAG TTGTCTGTGG ATCACATTAA ACAAATCATC
    TGCATCTTGA GAGACTTCAT ACGCTGCCCT GAACGTCGTG TTATGGCCAC CACCATCTCA
    AAACTCAAAC TGGACCTGGA CTTTGATAGC ACATCGATCA ACCAGATTCA ACTCGTTCTG
    TTCGGCTTTC AGGACTCTGT GAATAAAGTG TTACGGAACC ACCACATTAA ACCACACTGG
    ATGTTTGCCA TGGATAACAT CATCAGACGG GCTGTGCAGG CAGCTGTTAC CATTTTAATT
    CCAGAGCTGC AGACACATTT TGGTCCCGCA TCAGAGAGCG AGGGCGCAGA TAAAGACGCA
    GATGAAGTAG ATGAAGAGGA CGATGCAGAC TTTGGATCAG TAGCACGTAC AACACAGGAA
    GATCAAGTTC TCACATCTGG AGTCAGCACA CTTAGTTCAG TCATATCCCA TGATGCGCAG
    CGGCTACAGC ACCCCCTAGG AGCACAGCTG GGACGACTCA AACAGGAAAC TTGCAGGCTT
    CTGGAGGAGC TGGTGCAGAA GGAGAGAGAA TATCAGCAGG TTCTGAGACA AACTCTCCAA
    CAGCGGGTAC ATGACATCGA GCTTATCAGG ATCAGACAGC AGCCTGCTGC AGCAGAGATG
    CCCACTACAC CCTCAGTATT TCACGTTACT GCACAACCCG AGCCTGACAA GAAGCTGACG
    GATTGGCTGA AGGAGCAGGG AGCAGACAGC GAGACGATAG ACAAGTTTGT TGCAGAAGAT
    TATTCACTCA GCGACGTTCT CTGCGATATT ACTAAAGAAG ATCTTCGATG CCTGAGGCTT
    AGGGGTGGAA TGCTGTGTCG GATCTGGAAA GCCATTCAAC AACATCGCAA CAAAGGACTT
    CGAAAGGTCA AAAACGACGA GGGAAATTCA GAAGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa28664
    Clone ID Related Accession (Same CDS sequence) XM_021473796.1 , XM_009297482.2 , XM_009297482.3
    Accession Version XM_009297482.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3999bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product mitogen-activated protein kinase kinase kinase 15 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297482.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGATCA TGTCCAATCT ACCTACAGGA GAGGCTGGTG GCGACCACAC CAATGCGGTT 
    TCCATGTCTG CGGGCATGGA GAGAGCCGAT GTGACCAGCC CGAGTCCGGT GAGCAAACAG
    AGGTCCCTGC GGGCTGTGTA TGTGCTGAAT GATGGACTGA AGGCAGTGGC TGCGAATAGC
    CCGGAGTCCG GGGCTCTCCA GTGTCTCCAG CGCGCCTGCG ACGCGGAGAG TGCCATATTG
    ACCACAGTCA CCTTCGGACG ATTAGACTTT GGGGAGACCA CTGTGCTTGA TACTTTTTAC
    GATGCAGATA TTGCAGTTGT GGACATGAGT GATGTGTTCA GACAGCCCTC TTTGTTTTAT
    CACCTCGGGG TCCGTGAGAG CTTTGACATG GCCAACAATG TTATCCTCTA CCACGACATG
    GACCCCGACA CAGCACAGTC CCTTAAGGAC ATGGTGGCAC AGAAAAACAC AGCCTCTAGT
    GGGAACTACT ATTTCATCCC ATACCTGATG ACACCCAACA ACGAGTACTT GTGCTGTGAG
    AATGTGGCCC AGCGCAGAGC ATCAGAGTAC ATGCAGCCCA ACTGGGACAA CCTTTTAGGA
    CCTCTGTGTG TCCCTTTGGT GGACCGCTTC GTCAGCCTTC TCAAAGACAT TCATGTCACG
    TCTTGTGCTT CATACAAAGA TGCACTCCTG AATGACATCC GAAAAGCTCG AGATAAATAC
    CAGGGTGAGG AGCTGGCCAA AGAGCTTTCC CGTATCAAAC TCCGCATCGA CAACACTGAG
    GTTTTGACTC AAGATATTGT CATGAACCTG CTTTTCTCCT ACAGAGACAT ACAGGATTAT
    GATGCTATGG TTAAACTGGT GCAGACCATT GAGATGTTGC CCACCTGTGA CTTGGCCAAC
    CAGCCCATGA TTCAGTTCCA CTATGCTTTT GCGCTCAATA GACGAAACAG CCCTGGTGAT
    CGAGAGCAGG CGCTGCGTGT GATGTTGCAG GTTCTTCAAT CGTGTGAACA CCCTGCTCCT
    GACATGTTCT GCTTGTGTGG CCGCATATAT AAAGACATCT TTCTAGACTC AGACTGTAAA
    GACACCAAAA ACAGAGACAA CGCCATCCAG TGGTATAGAA AAGGTTTCGA GCTTCAGCCC
    ACGCTCTATT CAGGGATTAA TCTAGCAGTG CTGCTTATAG TAGCCGGGCA GCAGTTCGAG
    ACTTCCATAG AACTCAGGAA GATCGGTGTA AGGTTGAACA GTCTGCTGGG CCGTAAAGGC
    AGTCTGGAGA AGATGAATAA CTACTGGGAC GTTGGACAGT TTTTTACTGT CAGCATGCTG
    GCCAATGACA TCCCCAAAGC TGTGCAGGCC GCTGAGAAAC TCTTCAAGCT CAAACCACCT
    ATATGGTACC TGCGGTCAGT GGTCCAAAAC CTCCAGCTGA TTCAGCATTT CAAGAAGCAG
    AACACTGAAC ACTCGCCTCA AAGAGAGAGA CTCAACTTCT GGATGGACAT TATCGTGGAG
    GCCACGCAGG GAACCACCAA CGGCCTTCGT TTTCCGGTGT TGATTCTGGA GCCCACTAAA
    GTTTACCAGC CATCTTATGT ATCGATTAAC AGTGAAGCAG AAGAGAAGAA TGTGTCTATA
    TGGCACGTGT CGCCCGCTGA GACGAAGGGA ATACACGAGT GGAACTTCAC TGCGTCGTCC
    ATTAAAGGCA TCAGTATCTC AAAGTTTGAT GAGCGCTGCT GCTTTCTGTA CGTCCATGAT
    AATTCAGATG ACTTCCAGAT CTACTTCTCC ACAGAGGACC AGTGCGGCAG GTTCTGCTCA
    ATGGTGAAAG AGCTCATCAC AGATGGATCT GGAAATGCTG TTGAGCTGGA GGGCGAAGGA
    GACGGAGACA CCTTGGAGTA TGAGTATGAC TACAATGAGA ACGGTGACCG GGTGGTTCTG
    GGCAGGGGGA CCTATGGAGT TGTCTACGCA GGGAGAGACC TGAGCAATCA GGTGCGCATT
    GCCATCAAGG AGATCCCTGA GAGAGACAGC AGGTATTCCC AACCACTGCA TGAGGAAATC
    GCCCTTCACA AGTACCTGAA ACACAGAAAC ATAGTTCAGT ACCTGGGCTC AGTGTCAGAG
    GATGGTTATA TCAAGATCTT CATGGAGCAG GTCCCTGGAG GAAGTCTGTC AGCGTTGTTG
    CGTTCAAAGT GGGGTCCTTT GAAAGAAGCC ACCATAATCT TTTACACTCG GCAGATCCTG
    GAAGGCATTC GTTATTTGCA TGAAAACCAA ATAGTTCATC GGGATATCAA AGGAGACAAT
    GTTTTAGTCA ACACTTACAG TGGCGTGTTG AAAATCTCCG ACTTCGGCAC GTCAAAGAGA
    CTGGCTGGAG TCAATCCCTG CACTGAGACC TTCACTGGCA CCTTGCAGTA TATGGCCCCT
    GAGATCATCG ATAAGGGACC CAGAGGTTAT GGGGCCCCTG CTGACATCTG GTCTTTGGGC
    TGCACCATCA TTGAGATGGC GACAGGCAAA CCTCCTTTTC ATGAGCTGGG AGAGCCACAG
    GCCGCCATGT TTAAGGTGGG CATGTTTAAG ATCCATCCGG AGATCCCGGA GTCTCTTTCG
    TCGGAGGCAA AATCCTTCAT CCTGTCCAGC TTTGAGCCTG ATCCCAATAA ACGAGCAATG
    GCCGGCGACC TGCTCAAAGA CCCCTTCCTG CGGCAGAACA TCAAAGGCAA GAAGAACAAG
    ATCGCTTTCA AACCCTCAGA CTACATCCGC AGTGTTTCTC TACCCGTCCA GCTGCAGACA
    GAAGCCACAG GCAGCAGCAG CAGCGAGCCG GGCTCCGTCT CTCCAGACTG CGACTCCAAA
    CAAGATGTGT TTTTCGAGAA GAAGAAGCGT TCAAGCTCCG AAAACCTCAT TAAACCACCC
    AGCTCAACCT TTCTCAGTGT TCCAGATGAG AGTCCGACAG CAGAAGACCG TAGCTCTCCA
    GCCTCCTCTG AGAACAGCGA CTCTGGTCTC TTCCTGCTCA AGAAAGACAG TGAAAGACGG
    GCCATTCTCT ACAAGGTCCT CAATGAAGAC CAGGATAAAG TAACCTCCAA CCTCATGGAG
    AACCACATCC AGGGTCAAGA GGAGCTGAAG TTGTCTGTGG ATCACATTAA ACAAATCATC
    TGCATCTTGA GAGACTTCAT ACGCTGCCCT GAACGTCGTG TTATGGCCAC CACCATCTCA
    AAACTCAAAC TGGACCTGGA CTTTGATAGC ACATCGATCA ACCAGATTCA ACTCGTTCTG
    TTCGGCTTTC AGGACTCTGT GAATAAAGTG TTACGGAACC ACCACATTAA ACCACACTGG
    ATGTTTGCCA TGGATAACAT CATCAGACGG GCTGTGCAGG CAGCTGTTAC CATTTTAATT
    CCAGAGCTGC AGACACATTT TGGTCCCGCA TCAGAGAGCG AGGGCGCAGA TAAAGACGCA
    GATGAAGTAG ATGAAGAGGA CGATGCAGAC TTTGGATCAG TAGCACGTAC AACACAGGAA
    GATCAAGTTC TCACATCTGG AGTCAGCACA CTTAGTTCAG TCATATCCCA TGATGCGCAG
    CGGCTACAGC ACCCCCTAGG AGCACAGCTG GGACGACTCA AACAGGAAAC TTGCAGGCTT
    CTGGAGGAGC TGGTGCAGAA GGAGAGAGAA TATCAGCAGG TTCTGAGACA AACTCTCCAA
    CAGCGGGTAC ATGACATCGA GCTTATCAGG ATCAGACAGC AGCCTGCTGC AGCAGAGATG
    CCCACTACAC CCTCAGTATT TCACGTTACT GCACAACCCG AGCCTGACAA GAAGCTGACG
    GATTGGCTGA AGGAGCAGGG AGCAGACAGC GAGACGATAG ACAAGTTTGT TGCAGAAGAT
    TATTCACTCA GCGACGTTCT CTGCGATATT ACTAAAGAAG ATCTTCGATG CCTGAGGCTT
    AGGGGTGGAA TGCTGTGTCG GATCTGGAAA GCCATTCAAC AACATCGCAA CAAAGGACTT
    CGAAAGGTCA AAAACGACGA GGGAAATTCA GAAGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295757.1
    CDS683..4681
    Translation

    Target ORF information:

    RefSeq Version XM_009297482.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio mitogen-activated protein kinase kinase kinase 15 (map3k15), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009297482.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGATCA TGTCCAATCT ACCTACAGGA GAGGCTGGTG GCGACCACAC CAATGCGGTT 
    TCCATGTCTG CGGGCATGGA GAGAGCCGAT GTGACCAGCC CGAGTCCGGT GAGCAAACAG
    AGGTCCCTGC GGGCTGTGTA TGTGCTGAAT GATGGACTGA AGGCAGTGGC TGCGAATAGC
    CCGGAGTCCG GGGCTCTCCA GTGTCTCCAG CGCGCCTGCG ACGCGGAGAG TGCCATATTG
    ACCACAGTCA CCTTCGGACG ATTAGACTTT GGGGAGACCA CTGTGCTTGA TACTTTTTAC
    GATGCAGATA TTGCAGTTGT GGACATGAGT GATGTGTTCA GACAGCCCTC TTTGTTTTAT
    CACCTCGGGG TCCGTGAGAG CTTTGACATG GCCAACAATG TTATCCTCTA CCACGACATG
    GACCCCGACA CAGCACAGTC CCTTAAGGAC ATGGTGGCAC AGAAAAACAC AGCCTCTAGT
    GGGAACTACT ATTTCATCCC ATACCTGATG ACACCCAACA ACGAGTACTT GTGCTGTGAG
    AATGTGGCCC AGCGCAGAGC ATCAGAGTAC ATGCAGCCCA ACTGGGACAA CCTTTTAGGA
    CCTCTGTGTG TCCCTTTGGT GGACCGCTTC GTCAGCCTTC TCAAAGACAT TCATGTCACG
    TCTTGTGCTT CATACAAAGA TGCACTCCTG AATGACATCC GAAAAGCTCG AGATAAATAC
    CAGGGTGAGG AGCTGGCCAA AGAGCTTTCC CGTATCAAAC TCCGCATCGA CAACACTGAG
    GTTTTGACTC AAGATATTGT CATGAACCTG CTTTTCTCCT ACAGAGACAT ACAGGATTAT
    GATGCTATGG TTAAACTGGT GCAGACCATT GAGATGTTGC CCACCTGTGA CTTGGCCAAC
    CAGCCCATGA TTCAGTTCCA CTATGCTTTT GCGCTCAATA GACGAAACAG CCCTGGTGAT
    CGAGAGCAGG CGCTGCGTGT GATGTTGCAG GTTCTTCAAT CGTGTGAACA CCCTGCTCCT
    GACATGTTCT GCTTGTGTGG CCGCATATAT AAAGACATCT TTCTAGACTC AGACTGTAAA
    GACACCAAAA ACAGAGACAA CGCCATCCAG TGGTATAGAA AAGGTTTCGA GCTTCAGCCC
    ACGCTCTATT CAGGGATTAA TCTAGCAGTG CTGCTTATAG TAGCCGGGCA GCAGTTCGAG
    ACTTCCATAG AACTCAGGAA GATCGGTGTA AGGTTGAACA GTCTGCTGGG CCGTAAAGGC
    AGTCTGGAGA AGATGAATAA CTACTGGGAC GTTGGACAGT TTTTTACTGT CAGCATGCTG
    GCCAATGACA TCCCCAAAGC TGTGCAGGCC GCTGAGAAAC TCTTCAAGCT CAAACCACCT
    ATATGGTACC TGCGGTCAGT GGTCCAAAAC CTCCAGCTGA TTCAGCATTT CAAGAAGCAG
    AACACTGAAC ACTCGCCTCA AAGAGAGAGA CTCAACTTCT GGATGGACAT TATCGTGGAG
    GCCACGCAGG GAACCACCAA CGGCCTTCGT TTTCCGGTGT TGATTCTGGA GCCCACTAAA
    GTTTACCAGC CATCTTATGT ATCGATTAAC AGTGAAGCAG AAGAGAAGAA TGTGTCTATA
    TGGCACGTGT CGCCCGCTGA GACGAAGGGA ATACACGAGT GGAACTTCAC TGCGTCGTCC
    ATTAAAGGCA TCAGTATCTC AAAGTTTGAT GAGCGCTGCT GCTTTCTGTA CGTCCATGAT
    AATTCAGATG ACTTCCAGAT CTACTTCTCC ACAGAGGACC AGTGCGGCAG GTTCTGCTCA
    ATGGTGAAAG AGCTCATCAC AGATGGATCT GGAAATGCTG TTGAGCTGGA GGGCGAAGGA
    GACGGAGACA CCTTGGAGTA TGAGTATGAC TACAATGAGA ACGGTGACCG GGTGGTTCTG
    GGCAGGGGGA CCTATGGAGT TGTCTACGCA GGGAGAGACC TGAGCAATCA GGTGCGCATT
    GCCATCAAGG AGATCCCTGA GAGAGACAGC AGGTATTCCC AACCACTGCA TGAGGAAATC
    GCCCTTCACA AGTACCTGAA ACACAGAAAC ATAGTTCAGT ACCTGGGCTC AGTGTCAGAG
    GATGGTTATA TCAAGATCTT CATGGAGCAG GTCCCTGGAG GAAGTCTGTC AGCGTTGTTG
    CGTTCAAAGT GGGGTCCTTT GAAAGAAGCC ACCATAATCT TTTACACTCG GCAGATCCTG
    GAAGGCATTC GTTATTTGCA TGAAAACCAA ATAGTTCATC GGGATATCAA AGGAGACAAT
    GTTTTAGTCA ACACTTACAG TGGCGTGTTG AAAATCTCCG ACTTCGGCAC GTCAAAGAGA
    CTGGCTGGAG TCAATCCCTG CACTGAGACC TTCACTGGCA CCTTGCAGTA TATGGCCCCT
    GAGATCATCG ATAAGGGACC CAGAGGTTAT GGGGCCCCTG CTGACATCTG GTCTTTGGGC
    TGCACCATCA TTGAGATGGC GACAGGCAAA CCTCCTTTTC ATGAGCTGGG AGAGCCACAG
    GCCGCCATGT TTAAGGTGGG CATGTTTAAG ATCCATCCGG AGATCCCGGA GTCTCTTTCG
    TCGGAGGCAA AATCCTTCAT CCTGTCCAGC TTTGAGCCTG ATCCCAATAA ACGAGCAATG
    GCCGGCGACC TGCTCAAAGA CCCCTTCCTG CGGCAGAACA TCAAAGGCAA GAAGAACAAG
    ATCGCTTTCA AACCCTCAGA CTACATCCGC AGTGTTTCTC TACCCGTCCA GCTGCAGACA
    GAAGCCACAG GCAGCAGCAG CAGCGAGCCG GGCTCCGTCT CTCCAGACTG CGACTCCAAA
    CAAGATGTGT TTTTCGAGAA GAAGAAGCGT TCAAGCTCCG AAAACCTCAT TAAACCACCC
    AGCTCAACCT TTCTCAGTGT TCCAGATGAG AGTCCGACAG CAGAAGACCG TAGCTCTCCA
    GCCTCCTCTG AGAACAGCGA CTCTGGTCTC TTCCTGCTCA AGAAAGACAG TGAAAGACGG
    GCCATTCTCT ACAAGGTCCT CAATGAAGAC CAGGATAAAG TAACCTCCAA CCTCATGGAG
    AACCACATCC AGGGTCAAGA GGAGCTGAAG TTGTCTGTGG ATCACATTAA ACAAATCATC
    TGCATCTTGA GAGACTTCAT ACGCTGCCCT GAACGTCGTG TTATGGCCAC CACCATCTCA
    AAACTCAAAC TGGACCTGGA CTTTGATAGC ACATCGATCA ACCAGATTCA ACTCGTTCTG
    TTCGGCTTTC AGGACTCTGT GAATAAAGTG TTACGGAACC ACCACATTAA ACCACACTGG
    ATGTTTGCCA TGGATAACAT CATCAGACGG GCTGTGCAGG CAGCTGTTAC CATTTTAATT
    CCAGAGCTGC AGACACATTT TGGTCCCGCA TCAGAGAGCG AGGGCGCAGA TAAAGACGCA
    GATGAAGTAG ATGAAGAGGA CGATGCAGAC TTTGGATCAG TAGCACGTAC AACACAGGAA
    GATCAAGTTC TCACATCTGG AGTCAGCACA CTTAGTTCAG TCATATCCCA TGATGCGCAG
    CGGCTACAGC ACCCCCTAGG AGCACAGCTG GGACGACTCA AACAGGAAAC TTGCAGGCTT
    CTGGAGGAGC TGGTGCAGAA GGAGAGAGAA TATCAGCAGG TTCTGAGACA AACTCTCCAA
    CAGCGGGTAC ATGACATCGA GCTTATCAGG ATCAGACAGC AGCCTGCTGC AGCAGAGATG
    CCCACTACAC CCTCAGTATT TCACGTTACT GCACAACCCG AGCCTGACAA GAAGCTGACG
    GATTGGCTGA AGGAGCAGGG AGCAGACAGC GAGACGATAG ACAAGTTTGT TGCAGAAGAT
    TATTCACTCA GCGACGTTCT CTGCGATATT ACTAAAGAAG ATCTTCGATG CCTGAGGCTT
    AGGGGTGGAA TGCTGTGTCG GATCTGGAAA GCCATTCAAC AACATCGCAA CAAAGGACTT
    CGAAAGGTCA AAAACGACGA GGGAAATTCA GAAGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa28664
    Clone ID Related Accession (Same CDS sequence) XM_021473796.1 , XM_009297482.2 , XM_009297482.3
    Accession Version XM_009297482.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3999bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product mitogen-activated protein kinase kinase kinase 15 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009297482.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGATCA TGTCCAATCT ACCTACAGGA GAGGCTGGTG GCGACCACAC CAATGCGGTT 
    TCCATGTCTG CGGGCATGGA GAGAGCCGAT GTGACCAGCC CGAGTCCGGT GAGCAAACAG
    AGGTCCCTGC GGGCTGTGTA TGTGCTGAAT GATGGACTGA AGGCAGTGGC TGCGAATAGC
    CCGGAGTCCG GGGCTCTCCA GTGTCTCCAG CGCGCCTGCG ACGCGGAGAG TGCCATATTG
    ACCACAGTCA CCTTCGGACG ATTAGACTTT GGGGAGACCA CTGTGCTTGA TACTTTTTAC
    GATGCAGATA TTGCAGTTGT GGACATGAGT GATGTGTTCA GACAGCCCTC TTTGTTTTAT
    CACCTCGGGG TCCGTGAGAG CTTTGACATG GCCAACAATG TTATCCTCTA CCACGACATG
    GACCCCGACA CAGCACAGTC CCTTAAGGAC ATGGTGGCAC AGAAAAACAC AGCCTCTAGT
    GGGAACTACT ATTTCATCCC ATACCTGATG ACACCCAACA ACGAGTACTT GTGCTGTGAG
    AATGTGGCCC AGCGCAGAGC ATCAGAGTAC ATGCAGCCCA ACTGGGACAA CCTTTTAGGA
    CCTCTGTGTG TCCCTTTGGT GGACCGCTTC GTCAGCCTTC TCAAAGACAT TCATGTCACG
    TCTTGTGCTT CATACAAAGA TGCACTCCTG AATGACATCC GAAAAGCTCG AGATAAATAC
    CAGGGTGAGG AGCTGGCCAA AGAGCTTTCC CGTATCAAAC TCCGCATCGA CAACACTGAG
    GTTTTGACTC AAGATATTGT CATGAACCTG CTTTTCTCCT ACAGAGACAT ACAGGATTAT
    GATGCTATGG TTAAACTGGT GCAGACCATT GAGATGTTGC CCACCTGTGA CTTGGCCAAC
    CAGCCCATGA TTCAGTTCCA CTATGCTTTT GCGCTCAATA GACGAAACAG CCCTGGTGAT
    CGAGAGCAGG CGCTGCGTGT GATGTTGCAG GTTCTTCAAT CGTGTGAACA CCCTGCTCCT
    GACATGTTCT GCTTGTGTGG CCGCATATAT AAAGACATCT TTCTAGACTC AGACTGTAAA
    GACACCAAAA ACAGAGACAA CGCCATCCAG TGGTATAGAA AAGGTTTCGA GCTTCAGCCC
    ACGCTCTATT CAGGGATTAA TCTAGCAGTG CTGCTTATAG TAGCCGGGCA GCAGTTCGAG
    ACTTCCATAG AACTCAGGAA GATCGGTGTA AGGTTGAACA GTCTGCTGGG CCGTAAAGGC
    AGTCTGGAGA AGATGAATAA CTACTGGGAC GTTGGACAGT TTTTTACTGT CAGCATGCTG
    GCCAATGACA TCCCCAAAGC TGTGCAGGCC GCTGAGAAAC TCTTCAAGCT CAAACCACCT
    ATATGGTACC TGCGGTCAGT GGTCCAAAAC CTCCAGCTGA TTCAGCATTT CAAGAAGCAG
    AACACTGAAC ACTCGCCTCA AAGAGAGAGA CTCAACTTCT GGATGGACAT TATCGTGGAG
    GCCACGCAGG GAACCACCAA CGGCCTTCGT TTTCCGGTGT TGATTCTGGA GCCCACTAAA
    GTTTACCAGC CATCTTATGT ATCGATTAAC AGTGAAGCAG AAGAGAAGAA TGTGTCTATA
    TGGCACGTGT CGCCCGCTGA GACGAAGGGA ATACACGAGT GGAACTTCAC TGCGTCGTCC
    ATTAAAGGCA TCAGTATCTC AAAGTTTGAT GAGCGCTGCT GCTTTCTGTA CGTCCATGAT
    AATTCAGATG ACTTCCAGAT CTACTTCTCC ACAGAGGACC AGTGCGGCAG GTTCTGCTCA
    ATGGTGAAAG AGCTCATCAC AGATGGATCT GGAAATGCTG TTGAGCTGGA GGGCGAAGGA
    GACGGAGACA CCTTGGAGTA TGAGTATGAC TACAATGAGA ACGGTGACCG GGTGGTTCTG
    GGCAGGGGGA CCTATGGAGT TGTCTACGCA GGGAGAGACC TGAGCAATCA GGTGCGCATT
    GCCATCAAGG AGATCCCTGA GAGAGACAGC AGGTATTCCC AACCACTGCA TGAGGAAATC
    GCCCTTCACA AGTACCTGAA ACACAGAAAC ATAGTTCAGT ACCTGGGCTC AGTGTCAGAG
    GATGGTTATA TCAAGATCTT CATGGAGCAG GTCCCTGGAG GAAGTCTGTC AGCGTTGTTG
    CGTTCAAAGT GGGGTCCTTT GAAAGAAGCC ACCATAATCT TTTACACTCG GCAGATCCTG
    GAAGGCATTC GTTATTTGCA TGAAAACCAA ATAGTTCATC GGGATATCAA AGGAGACAAT
    GTTTTAGTCA ACACTTACAG TGGCGTGTTG AAAATCTCCG ACTTCGGCAC GTCAAAGAGA
    CTGGCTGGAG TCAATCCCTG CACTGAGACC TTCACTGGCA CCTTGCAGTA TATGGCCCCT
    GAGATCATCG ATAAGGGACC CAGAGGTTAT GGGGCCCCTG CTGACATCTG GTCTTTGGGC
    TGCACCATCA TTGAGATGGC GACAGGCAAA CCTCCTTTTC ATGAGCTGGG AGAGCCACAG
    GCCGCCATGT TTAAGGTGGG CATGTTTAAG ATCCATCCGG AGATCCCGGA GTCTCTTTCG
    TCGGAGGCAA AATCCTTCAT CCTGTCCAGC TTTGAGCCTG ATCCCAATAA ACGAGCAATG
    GCCGGCGACC TGCTCAAAGA CCCCTTCCTG CGGCAGAACA TCAAAGGCAA GAAGAACAAG
    ATCGCTTTCA AACCCTCAGA CTACATCCGC AGTGTTTCTC TACCCGTCCA GCTGCAGACA
    GAAGCCACAG GCAGCAGCAG CAGCGAGCCG GGCTCCGTCT CTCCAGACTG CGACTCCAAA
    CAAGATGTGT TTTTCGAGAA GAAGAAGCGT TCAAGCTCCG AAAACCTCAT TAAACCACCC
    AGCTCAACCT TTCTCAGTGT TCCAGATGAG AGTCCGACAG CAGAAGACCG TAGCTCTCCA
    GCCTCCTCTG AGAACAGCGA CTCTGGTCTC TTCCTGCTCA AGAAAGACAG TGAAAGACGG
    GCCATTCTCT ACAAGGTCCT CAATGAAGAC CAGGATAAAG TAACCTCCAA CCTCATGGAG
    AACCACATCC AGGGTCAAGA GGAGCTGAAG TTGTCTGTGG ATCACATTAA ACAAATCATC
    TGCATCTTGA GAGACTTCAT ACGCTGCCCT GAACGTCGTG TTATGGCCAC CACCATCTCA
    AAACTCAAAC TGGACCTGGA CTTTGATAGC ACATCGATCA ACCAGATTCA ACTCGTTCTG
    TTCGGCTTTC AGGACTCTGT GAATAAAGTG TTACGGAACC ACCACATTAA ACCACACTGG
    ATGTTTGCCA TGGATAACAT CATCAGACGG GCTGTGCAGG CAGCTGTTAC CATTTTAATT
    CCAGAGCTGC AGACACATTT TGGTCCCGCA TCAGAGAGCG AGGGCGCAGA TAAAGACGCA
    GATGAAGTAG ATGAAGAGGA CGATGCAGAC TTTGGATCAG TAGCACGTAC AACACAGGAA
    GATCAAGTTC TCACATCTGG AGTCAGCACA CTTAGTTCAG TCATATCCCA TGATGCGCAG
    CGGCTACAGC ACCCCCTAGG AGCACAGCTG GGACGACTCA AACAGGAAAC TTGCAGGCTT
    CTGGAGGAGC TGGTGCAGAA GGAGAGAGAA TATCAGCAGG TTCTGAGACA AACTCTCCAA
    CAGCGGGTAC ATGACATCGA GCTTATCAGG ATCAGACAGC AGCCTGCTGC AGCAGAGATG
    CCCACTACAC CCTCAGTATT TCACGTTACT GCACAACCCG AGCCTGACAA GAAGCTGACG
    GATTGGCTGA AGGAGCAGGG AGCAGACAGC GAGACGATAG ACAAGTTTGT TGCAGAAGAT
    TATTCACTCA GCGACGTTCT CTGCGATATT ACTAAAGAAG ATCTTCGATG CCTGAGGCTT
    AGGGGTGGAA TGCTGTGTCG GATCTGGAAA GCCATTCAAC AACATCGCAA CAAAGGACTT
    CGAAAGGTCA AAAACGACGA GGGAAATTCA GAAGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295757.1
    CDS774..4772
    Translation

    Target ORF information:

    RefSeq Version XM_009297482.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio mitogen-activated protein kinase kinase kinase 15 (map3k15), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009297482.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGATCA TGTCCAATCT ACCTACAGGA GAGGCTGGTG GCGACCACAC CAATGCGGTT 
    TCCATGTCTG CGGGCATGGA GAGAGCCGAT GTGACCAGCC CGAGTCCGGT GAGCAAACAG
    AGGTCCCTGC GGGCTGTGTA TGTGCTGAAT GATGGACTGA AGGCAGTGGC TGCGAATAGC
    CCGGAGTCCG GGGCTCTCCA GTGTCTCCAG CGCGCCTGCG ACGCGGAGAG TGCCATATTG
    ACCACAGTCA CCTTCGGACG ATTAGACTTT GGGGAGACCA CTGTGCTTGA TACTTTTTAC
    GATGCAGATA TTGCAGTTGT GGACATGAGT GATGTGTTCA GACAGCCCTC TTTGTTTTAT
    CACCTCGGGG TCCGTGAGAG CTTTGACATG GCCAACAATG TTATCCTCTA CCACGACATG
    GACCCCGACA CAGCACAGTC CCTTAAGGAC ATGGTGGCAC AGAAAAACAC AGCCTCTAGT
    GGGAACTACT ATTTCATCCC ATACCTGATG ACACCCAACA ACGAGTACTT GTGCTGTGAG
    AATGTGGCCC AGCGCAGAGC ATCAGAGTAC ATGCAGCCCA ACTGGGACAA CCTTTTAGGA
    CCTCTGTGTG TCCCTTTGGT GGACCGCTTC GTCAGCCTTC TCAAAGACAT TCATGTCACG
    TCTTGTGCTT CATACAAAGA TGCACTCCTG AATGACATCC GAAAAGCTCG AGATAAATAC
    CAGGGTGAGG AGCTGGCCAA AGAGCTTTCC CGTATCAAAC TCCGCATCGA CAACACTGAG
    GTTTTGACTC AAGATATTGT CATGAACCTG CTTTTCTCCT ACAGAGACAT ACAGGATTAT
    GATGCTATGG TTAAACTGGT GCAGACCATT GAGATGTTGC CCACCTGTGA CTTGGCCAAC
    CAGCCCATGA TTCAGTTCCA CTATGCTTTT GCGCTCAATA GACGAAACAG CCCTGGTGAT
    CGAGAGCAGG CGCTGCGTGT GATGTTGCAG GTTCTTCAAT CGTGTGAACA CCCTGCTCCT
    GACATGTTCT GCTTGTGTGG CCGCATATAT AAAGACATCT TTCTAGACTC AGACTGTAAA
    GACACCAAAA ACAGAGACAA CGCCATCCAG TGGTATAGAA AAGGTTTCGA GCTTCAGCCC
    ACGCTCTATT CAGGGATTAA TCTAGCAGTG CTGCTTATAG TAGCCGGGCA GCAGTTCGAG
    ACTTCCATAG AACTCAGGAA GATCGGTGTA AGGTTGAACA GTCTGCTGGG CCGTAAAGGC
    AGTCTGGAGA AGATGAATAA CTACTGGGAC GTTGGACAGT TTTTTACTGT CAGCATGCTG
    GCCAATGACA TCCCCAAAGC TGTGCAGGCC GCTGAGAAAC TCTTCAAGCT CAAACCACCT
    ATATGGTACC TGCGGTCAGT GGTCCAAAAC CTCCAGCTGA TTCAGCATTT CAAGAAGCAG
    AACACTGAAC ACTCGCCTCA AAGAGAGAGA CTCAACTTCT GGATGGACAT TATCGTGGAG
    GCCACGCAGG GAACCACCAA CGGCCTTCGT TTTCCGGTGT TGATTCTGGA GCCCACTAAA
    GTTTACCAGC CATCTTATGT ATCGATTAAC AGTGAAGCAG AAGAGAAGAA TGTGTCTATA
    TGGCACGTGT CGCCCGCTGA GACGAAGGGA ATACACGAGT GGAACTTCAC TGCGTCGTCC
    ATTAAAGGCA TCAGTATCTC AAAGTTTGAT GAGCGCTGCT GCTTTCTGTA CGTCCATGAT
    AATTCAGATG ACTTCCAGAT CTACTTCTCC ACAGAGGACC AGTGCGGCAG GTTCTGCTCA
    ATGGTGAAAG AGCTCATCAC AGATGGATCT GGAAATGCTG TTGAGCTGGA GGGCGAAGGA
    GACGGAGACA CCTTGGAGTA TGAGTATGAC TACAATGAGA ACGGTGACCG GGTGGTTCTG
    GGCAGGGGGA CCTATGGAGT TGTCTACGCA GGGAGAGACC TGAGCAATCA GGTGCGCATT
    GCCATCAAGG AGATCCCTGA GAGAGACAGC AGGTATTCCC AACCACTGCA TGAGGAAATC
    GCCCTTCACA AGTACCTGAA ACACAGAAAC ATAGTTCAGT ACCTGGGCTC AGTGTCAGAG
    GATGGTTATA TCAAGATCTT CATGGAGCAG GTCCCTGGAG GAAGTCTGTC AGCGTTGTTG
    CGTTCAAAGT GGGGTCCTTT GAAAGAAGCC ACCATAATCT TTTACACTCG GCAGATCCTG
    GAAGGCATTC GTTATTTGCA TGAAAACCAA ATAGTTCATC GGGATATCAA AGGAGACAAT
    GTTTTAGTCA ACACTTACAG TGGCGTGTTG AAAATCTCCG ACTTCGGCAC GTCAAAGAGA
    CTGGCTGGAG TCAATCCCTG CACTGAGACC TTCACTGGCA CCTTGCAGTA TATGGCCCCT
    GAGATCATCG ATAAGGGACC CAGAGGTTAT GGGGCCCCTG CTGACATCTG GTCTTTGGGC
    TGCACCATCA TTGAGATGGC GACAGGCAAA CCTCCTTTTC ATGAGCTGGG AGAGCCACAG
    GCCGCCATGT TTAAGGTGGG CATGTTTAAG ATCCATCCGG AGATCCCGGA GTCTCTTTCG
    TCGGAGGCAA AATCCTTCAT CCTGTCCAGC TTTGAGCCTG ATCCCAATAA ACGAGCAATG
    GCCGGCGACC TGCTCAAAGA CCCCTTCCTG CGGCAGAACA TCAAAGGCAA GAAGAACAAG
    ATCGCTTTCA AACCCTCAGA CTACATCCGC AGTGTTTCTC TACCCGTCCA GCTGCAGACA
    GAAGCCACAG GCAGCAGCAG CAGCGAGCCG GGCTCCGTCT CTCCAGACTG CGACTCCAAA
    CAAGATGTGT TTTTCGAGAA GAAGAAGCGT TCAAGCTCCG AAAACCTCAT TAAACCACCC
    AGCTCAACCT TTCTCAGTGT TCCAGATGAG AGTCCGACAG CAGAAGACCG TAGCTCTCCA
    GCCTCCTCTG AGAACAGCGA CTCTGGTCTC TTCCTGCTCA AGAAAGACAG TGAAAGACGG
    GCCATTCTCT ACAAGGTCCT CAATGAAGAC CAGGATAAAG TAACCTCCAA CCTCATGGAG
    AACCACATCC AGGGTCAAGA GGAGCTGAAG TTGTCTGTGG ATCACATTAA ACAAATCATC
    TGCATCTTGA GAGACTTCAT ACGCTGCCCT GAACGTCGTG TTATGGCCAC CACCATCTCA
    AAACTCAAAC TGGACCTGGA CTTTGATAGC ACATCGATCA ACCAGATTCA ACTCGTTCTG
    TTCGGCTTTC AGGACTCTGT GAATAAAGTG TTACGGAACC ACCACATTAA ACCACACTGG
    ATGTTTGCCA TGGATAACAT CATCAGACGG GCTGTGCAGG CAGCTGTTAC CATTTTAATT
    CCAGAGCTGC AGACACATTT TGGTCCCGCA TCAGAGAGCG AGGGCGCAGA TAAAGACGCA
    GATGAAGTAG ATGAAGAGGA CGATGCAGAC TTTGGATCAG TAGCACGTAC AACACAGGAA
    GATCAAGTTC TCACATCTGG AGTCAGCACA CTTAGTTCAG TCATATCCCA TGATGCGCAG
    CGGCTACAGC ACCCCCTAGG AGCACAGCTG GGACGACTCA AACAGGAAAC TTGCAGGCTT
    CTGGAGGAGC TGGTGCAGAA GGAGAGAGAA TATCAGCAGG TTCTGAGACA AACTCTCCAA
    CAGCGGGTAC ATGACATCGA GCTTATCAGG ATCAGACAGC AGCCTGCTGC AGCAGAGATG
    CCCACTACAC CCTCAGTATT TCACGTTACT GCACAACCCG AGCCTGACAA GAAGCTGACG
    GATTGGCTGA AGGAGCAGGG AGCAGACAGC GAGACGATAG ACAAGTTTGT TGCAGAAGAT
    TATTCACTCA GCGACGTTCT CTGCGATATT ACTAAAGAAG ATCTTCGATG CCTGAGGCTT
    AGGGGTGGAA TGCTGTGTCG GATCTGGAAA GCCATTCAAC AACATCGCAA CAAAGGACTT
    CGAAAGGTCA AAAACGACGA GGGAAATTCA GAAGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa28663
    Clone ID Related Accession (Same CDS sequence) XM_021473795.1 , XM_009297480.3 , XM_009297480.2
    Accession Version XM_021473795.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4008bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product mitogen-activated protein kinase kinase kinase 15 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395177.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGATCA TGTCCAATCT ACCTACAGGA GAGGCTGGTG GCGACCACAC CAATGCGGTT 
    TCCATGTCTG CGGGCATGGA GAGAGCCGAT GTGACCAGCC CGAGTCCGGT GAGCAAACAG
    AGGTCCCTGC GGGCTGTGTA TGTGCTGAAT GATGGACTGA AGGCAGTGGC TGCGAATAGC
    CCGGAGTCCG GGGCTCTCCA GTGTCTCCAG CGCGCCTGCG ACGCGGAGAG TGCCATATTG
    ACCACAGTCA CCTTCGGACG ATTAGACTTT GGGGAGACCA CTGTGCTTGA TACTTTTTAC
    GATGCAGATA TTGCAGTTGT GGACATGAGT GATGTGTTCA GACAGCCCTC TTTGTTTTAT
    CACCTCGGGG TCCGTGAGAG CTTTGACATG GCCAACAATG TTATCCTCTA CCACGACATG
    GACCCCGACA CAGCACAGTC CCTTAAGGAC ATGGTGGCAC AGAAAAACAC AGCCTCTAGT
    GGGAACTACT ATTTCATCCC ATACCTGATG ACACCCAACA ACGAGTACTT GTGCTGTGAG
    AATGTGGCCC AGCGCAGAGC ATCAGAGTAC ATGCAGCCCA ACTGGGACAA CCTTTTAGGA
    CCTCTGTGTG TCCCTTTGGT GGACCGCTTC GTCAGCCTTC TCAAAGACAT TCATGTCACG
    TCTTGTGCTT CATACAAAGA TGCACTCCTG AATGACATCC GAAAAGCTCG AGATAAATAC
    CAGGGTGAGG AGCTGGCCAA AGAGCTTTCC CGTATCAAAC TCCGCATCGA CAACACTGAG
    GTTTTGACTC AAGATATTGT CATGAACCTG CTTTTCTCCT ACAGAGACAT ACAGGATTAT
    GATGCTATGG TTAAACTGGT GCAGACCATT GAGATGTTGC CCACCTGTGA CTTGGCCAAC
    CAGCCCATGA TTCAGTTCCA CTATGCTTTT GCGCTCAATA GACGAAACAG CCCTGGTGAT
    CGAGAGCAGG CGCTGCGTGT GATGTTGCAG GTTCTTCAAT CGTGTGAACA CCCTGCTCCT
    GACATGTTCT GCTTGTGTGG CCGCATATAT AAAGACATCT TTCTAGACTC AGACTGTAAA
    GACACCAAAA ACAGAGACAA CGCCATCCAG TGGTATAGAA AAGGTTTCGA GCTTCAGCCC
    ACGCTCTATT CAGGGATTAA TCTAGCAGTG CTGCTTATAG TAGCCGGGCA GCAGTTCGAG
    ACTTCCATAG AACTCAGGAA GATCGGTGTA AGGTTGAACA GTCTGCTGGG CCGTAAAGGC
    AGTCTGGAGA AGATGAATAA CTACTGGGAC GTTGGACAGT TTTTTACTGT CAGCATGCTG
    GCCAATGACA TCCCCAAAGC TGTGCAGGCC GCTGAGAAAC TCTTCAAGCT CAAACCACCT
    ATATGGTACC TGCGGTCAGT GGTCCAAAAC CTCCAGCTGA TTCAGCATTT CAAGAAGCAG
    AACACTGAAC ACTCGCCTCA AAGAGAGAGA CTCAACTTCT GGATGGACAT TATCGTGGAG
    GCCACGCAGG GAACCACCAA CGGCCTTCGT TTTCCGGTGT TGATTCTGGA GCCCACTAAA
    GTTTACCAGC CATCTTATGT ATCGATTAAC AGTGAAGCAG AAGAGAAGAA TGTGTCTATA
    TGGCACGTGT CGCCCGCTGA GACGAAGGGA ATACACGAGT GGAACTTCAC TGCGTCGTCC
    ATTAAAGGCA TCAGTATCTC AAAGTTTGAT GAGCGCTGCT GCTTTCTGTA CGTCCATGAT
    AATTCAGATG ACTTCCAGAT CTACTTCTCC ACAGAGGACC AGTGCGGCAG GTTCTGCTCA
    ATGGTGAAAG AGCTCATCAC AGATGGATCT GGAAATGCTG TTGAGCTGGA GGGCGAAGGA
    GACGGAGACA CCTTGGAGTA TGAGTATGAC TACAATGAGA ACGGTGACCG GGTGGTTCTG
    GGCAGGGGGA CCTATGGAGT TGTCTACGCA GGGAGAGACC TGAGCAATCA GGTGCGCATT
    GCCATCAAGG AGATCCCTGA GAGAGACAGC AGGTATTCCC AACCACTGCA TGAGGAAATC
    GCCCTTCACA AGTACCTGAA ACACAGAAAC ATAGTTCAGT ACCTGGGCTC AGTGTCAGAG
    GATGGTTATA TCAAGATCTT CATGGAGCAG GTCCCTGGAG GAAGTCTGTC AGCGTTGTTG
    CGTTCAAAGT GGGGTCCTTT GAAAGAAGCC ACCATAATCT TTTACACTCG GCAGATCCTG
    GAAGGCATTC GTTATTTGCA TGAAAACCAA ATAGTTCATC GGGATATCAA AGGAGACAAT
    GTTTTAGTCA ACACTTACAG TGGCGTGTTG AAAATCTCCG ACTTCGGCAC GTCAAAGAGA
    CTGGCTGGAG TCAATCCCTG CACTGAGACC TTCACTGGCA CCTTGCAGTA TATGGCCCCT
    GAGATCATCG ATAAGGGACC CAGAGGTTAT GGGGCCCCTG CTGACATCTG GTCTTTGGGC
    TGCACCATCA TTGAGATGGC GACAGGCAAA CCTCCTTTTC ATGAGCTGGG AGAGCCACAG
    GCCGCCATGT TTAAGGTGGG CATGTTTAAG ATCCATCCGG AGATCCCGGA GTCTCTTTCG
    TCGGAGGCAA AATCCTTCAT CCTGTCCAGC TTTGAGCCTG ATCCCAATAA ACGAGCAATG
    GCCGGCGACC TGCTCAAAGA CCCCTTCCTG CGGCAGAACA TCAAAGGCAA GAAGAACAAG
    ATCGCTTTCA AACCCTCAGT GTGGCAAGAC TACATCCGCA GTGTTTCTCT ACCCGTCCAG
    CTGCAGACAG AAGCCACAGG CAGCAGCAGC AGCGAGCCGG GCTCCGTCTC TCCAGACTGC
    GACTCCAAAC AAGATGTGTT TTTCGAGAAG AAGAAGCGTT CAAGCTCCGA AAACCTCATT
    AAACCACCCA GCTCAACCTT TCTCAGTGTT CCAGATGAGA GTCCGACAGC AGAAGACCGT
    AGCTCTCCAG CCTCCTCTGA GAACAGCGAC TCTGGTCTCT TCCTGCTCAA GAAAGACAGT
    GAAAGACGGG CCATTCTCTA CAAGGTCCTC AATGAAGACC AGGATAAAGT AACCTCCAAC
    CTCATGGAGA ACCACATCCA GGGTCAAGAG GAGCTGAAGT TGTCTGTGGA TCACATTAAA
    CAAATCATCT GCATCTTGAG AGACTTCATA CGCTGCCCTG AACGTCGTGT TATGGCCACC
    ACCATCTCAA AACTCAAACT GGACCTGGAC TTTGATAGCA CATCGATCAA CCAGATTCAA
    CTCGTTCTGT TCGGCTTTCA GGACTCTGTG AATAAAGTGT TACGGAACCA CCACATTAAA
    CCACACTGGA TGTTTGCCAT GGATAACATC ATCAGACGGG CTGTGCAGGC AGCTGTTACC
    ATTTTAATTC CAGAGCTGCA GACACATTTT GGTCCCGCAT CAGAGAGCGA GGGCGCAGAT
    AAAGACGCAG ATGAAGTAGA TGAAGAGGAC GATGCAGACT TTGGATCAGT AGCACGTACA
    ACACAGGAAG ATCAAGTTCT CACATCTGGA GTCAGCACAC TTAGTTCAGT CATATCCCAT
    GATGCGCAGC GGCTACAGCA CCCCCTAGGA GCACAGCTGG GACGACTCAA ACAGGAAACT
    TGCAGGCTTC TGGAGGAGCT GGTGCAGAAG GAGAGAGAAT ATCAGCAGGT TCTGAGACAA
    ACTCTCCAAC AGCGGGTACA TGACATCGAG CTTATCAGGA TCAGACAGCA GCCTGCTGCA
    GCAGAGATGC CCACTACACC CTCAGTATTT CACGTTACTG CACAACCCGA GCCTGACAAG
    AAGCTGACGG ATTGGCTGAA GGAGCAGGGA GCAGACAGCG AGACGATAGA CAAGTTTGTT
    GCAGAAGATT ATTCACTCAG CGACGTTCTC TGCGATATTA CTAAAGAAGA TCTTCGATGC
    CTGAGGCTTA GGGGTGGAAT GCTGTGTCGG ATCTGGAAAG CCATTCAACA ACATCGCAAC
    AAAGGACTTC GAAAGGTCAA AAACGACGAG GGAAATTCAG AAGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021329470.1
    CDS776..4783
    Translation

    Target ORF information:

    RefSeq Version XM_021473795.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio mitogen-activated protein kinase kinase kinase 15 (map3k15), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021473795.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGATCA TGTCCAATCT ACCTACAGGA GAGGCTGGTG GCGACCACAC CAATGCGGTT 
    TCCATGTCTG CGGGCATGGA GAGAGCCGAT GTGACCAGCC CGAGTCCGGT GAGCAAACAG
    AGGTCCCTGC GGGCTGTGTA TGTGCTGAAT GATGGACTGA AGGCAGTGGC TGCGAATAGC
    CCGGAGTCCG GGGCTCTCCA GTGTCTCCAG CGCGCCTGCG ACGCGGAGAG TGCCATATTG
    ACCACAGTCA CCTTCGGACG ATTAGACTTT GGGGAGACCA CTGTGCTTGA TACTTTTTAC
    GATGCAGATA TTGCAGTTGT GGACATGAGT GATGTGTTCA GACAGCCCTC TTTGTTTTAT
    CACCTCGGGG TCCGTGAGAG CTTTGACATG GCCAACAATG TTATCCTCTA CCACGACATG
    GACCCCGACA CAGCACAGTC CCTTAAGGAC ATGGTGGCAC AGAAAAACAC AGCCTCTAGT
    GGGAACTACT ATTTCATCCC ATACCTGATG ACACCCAACA ACGAGTACTT GTGCTGTGAG
    AATGTGGCCC AGCGCAGAGC ATCAGAGTAC ATGCAGCCCA ACTGGGACAA CCTTTTAGGA
    CCTCTGTGTG TCCCTTTGGT GGACCGCTTC GTCAGCCTTC TCAAAGACAT TCATGTCACG
    TCTTGTGCTT CATACAAAGA TGCACTCCTG AATGACATCC GAAAAGCTCG AGATAAATAC
    CAGGGTGAGG AGCTGGCCAA AGAGCTTTCC CGTATCAAAC TCCGCATCGA CAACACTGAG
    GTTTTGACTC AAGATATTGT CATGAACCTG CTTTTCTCCT ACAGAGACAT ACAGGATTAT
    GATGCTATGG TTAAACTGGT GCAGACCATT GAGATGTTGC CCACCTGTGA CTTGGCCAAC
    CAGCCCATGA TTCAGTTCCA CTATGCTTTT GCGCTCAATA GACGAAACAG CCCTGGTGAT
    CGAGAGCAGG CGCTGCGTGT GATGTTGCAG GTTCTTCAAT CGTGTGAACA CCCTGCTCCT
    GACATGTTCT GCTTGTGTGG CCGCATATAT AAAGACATCT TTCTAGACTC AGACTGTAAA
    GACACCAAAA ACAGAGACAA CGCCATCCAG TGGTATAGAA AAGGTTTCGA GCTTCAGCCC
    ACGCTCTATT CAGGGATTAA TCTAGCAGTG CTGCTTATAG TAGCCGGGCA GCAGTTCGAG
    ACTTCCATAG AACTCAGGAA GATCGGTGTA AGGTTGAACA GTCTGCTGGG CCGTAAAGGC
    AGTCTGGAGA AGATGAATAA CTACTGGGAC GTTGGACAGT TTTTTACTGT CAGCATGCTG
    GCCAATGACA TCCCCAAAGC TGTGCAGGCC GCTGAGAAAC TCTTCAAGCT CAAACCACCT
    ATATGGTACC TGCGGTCAGT GGTCCAAAAC CTCCAGCTGA TTCAGCATTT CAAGAAGCAG
    AACACTGAAC ACTCGCCTCA AAGAGAGAGA CTCAACTTCT GGATGGACAT TATCGTGGAG
    GCCACGCAGG GAACCACCAA CGGCCTTCGT TTTCCGGTGT TGATTCTGGA GCCCACTAAA
    GTTTACCAGC CATCTTATGT ATCGATTAAC AGTGAAGCAG AAGAGAAGAA TGTGTCTATA
    TGGCACGTGT CGCCCGCTGA GACGAAGGGA ATACACGAGT GGAACTTCAC TGCGTCGTCC
    ATTAAAGGCA TCAGTATCTC AAAGTTTGAT GAGCGCTGCT GCTTTCTGTA CGTCCATGAT
    AATTCAGATG ACTTCCAGAT CTACTTCTCC ACAGAGGACC AGTGCGGCAG GTTCTGCTCA
    ATGGTGAAAG AGCTCATCAC AGATGGATCT GGAAATGCTG TTGAGCTGGA GGGCGAAGGA
    GACGGAGACA CCTTGGAGTA TGAGTATGAC TACAATGAGA ACGGTGACCG GGTGGTTCTG
    GGCAGGGGGA CCTATGGAGT TGTCTACGCA GGGAGAGACC TGAGCAATCA GGTGCGCATT
    GCCATCAAGG AGATCCCTGA GAGAGACAGC AGGTATTCCC AACCACTGCA TGAGGAAATC
    GCCCTTCACA AGTACCTGAA ACACAGAAAC ATAGTTCAGT ACCTGGGCTC AGTGTCAGAG
    GATGGTTATA TCAAGATCTT CATGGAGCAG GTCCCTGGAG GAAGTCTGTC AGCGTTGTTG
    CGTTCAAAGT GGGGTCCTTT GAAAGAAGCC ACCATAATCT TTTACACTCG GCAGATCCTG
    GAAGGCATTC GTTATTTGCA TGAAAACCAA ATAGTTCATC GGGATATCAA AGGAGACAAT
    GTTTTAGTCA ACACTTACAG TGGCGTGTTG AAAATCTCCG ACTTCGGCAC GTCAAAGAGA
    CTGGCTGGAG TCAATCCCTG CACTGAGACC TTCACTGGCA CCTTGCAGTA TATGGCCCCT
    GAGATCATCG ATAAGGGACC CAGAGGTTAT GGGGCCCCTG CTGACATCTG GTCTTTGGGC
    TGCACCATCA TTGAGATGGC GACAGGCAAA CCTCCTTTTC ATGAGCTGGG AGAGCCACAG
    GCCGCCATGT TTAAGGTGGG CATGTTTAAG ATCCATCCGG AGATCCCGGA GTCTCTTTCG
    TCGGAGGCAA AATCCTTCAT CCTGTCCAGC TTTGAGCCTG ATCCCAATAA ACGAGCAATG
    GCCGGCGACC TGCTCAAAGA CCCCTTCCTG CGGCAGAACA TCAAAGGCAA GAAGAACAAG
    ATCGCTTTCA AACCCTCAGT GTGGCAAGAC TACATCCGCA GTGTTTCTCT ACCCGTCCAG
    CTGCAGACAG AAGCCACAGG CAGCAGCAGC AGCGAGCCGG GCTCCGTCTC TCCAGACTGC
    GACTCCAAAC AAGATGTGTT TTTCGAGAAG AAGAAGCGTT CAAGCTCCGA AAACCTCATT
    AAACCACCCA GCTCAACCTT TCTCAGTGTT CCAGATGAGA GTCCGACAGC AGAAGACCGT
    AGCTCTCCAG CCTCCTCTGA GAACAGCGAC TCTGGTCTCT TCCTGCTCAA GAAAGACAGT
    GAAAGACGGG CCATTCTCTA CAAGGTCCTC AATGAAGACC AGGATAAAGT AACCTCCAAC
    CTCATGGAGA ACCACATCCA GGGTCAAGAG GAGCTGAAGT TGTCTGTGGA TCACATTAAA
    CAAATCATCT GCATCTTGAG AGACTTCATA CGCTGCCCTG AACGTCGTGT TATGGCCACC
    ACCATCTCAA AACTCAAACT GGACCTGGAC TTTGATAGCA CATCGATCAA CCAGATTCAA
    CTCGTTCTGT TCGGCTTTCA GGACTCTGTG AATAAAGTGT TACGGAACCA CCACATTAAA
    CCACACTGGA TGTTTGCCAT GGATAACATC ATCAGACGGG CTGTGCAGGC AGCTGTTACC
    ATTTTAATTC CAGAGCTGCA GACACATTTT GGTCCCGCAT CAGAGAGCGA GGGCGCAGAT
    AAAGACGCAG ATGAAGTAGA TGAAGAGGAC GATGCAGACT TTGGATCAGT AGCACGTACA
    ACACAGGAAG ATCAAGTTCT CACATCTGGA GTCAGCACAC TTAGTTCAGT CATATCCCAT
    GATGCGCAGC GGCTACAGCA CCCCCTAGGA GCACAGCTGG GACGACTCAA ACAGGAAACT
    TGCAGGCTTC TGGAGGAGCT GGTGCAGAAG GAGAGAGAAT ATCAGCAGGT TCTGAGACAA
    ACTCTCCAAC AGCGGGTACA TGACATCGAG CTTATCAGGA TCAGACAGCA GCCTGCTGCA
    GCAGAGATGC CCACTACACC CTCAGTATTT CACGTTACTG CACAACCCGA GCCTGACAAG
    AAGCTGACGG ATTGGCTGAA GGAGCAGGGA GCAGACAGCG AGACGATAGA CAAGTTTGTT
    GCAGAAGATT ATTCACTCAG CGACGTTCTC TGCGATATTA CTAAAGAAGA TCTTCGATGC
    CTGAGGCTTA GGGGTGGAAT GCTGTGTCGG ATCTGGAAAG CCATTCAACA ACATCGCAAC
    AAAGGACTTC GAAAGGTCAA AAACGACGAG GGAAATTCAG AAGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa28663
    Clone ID Related Accession (Same CDS sequence) XM_021473795.1 , XM_009297480.3 , XM_009297480.2
    Accession Version XM_009297480.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4008bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product mitogen-activated protein kinase kinase kinase 15 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009297480.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGATCA TGTCCAATCT ACCTACAGGA GAGGCTGGTG GCGACCACAC CAATGCGGTT 
    TCCATGTCTG CGGGCATGGA GAGAGCCGAT GTGACCAGCC CGAGTCCGGT GAGCAAACAG
    AGGTCCCTGC GGGCTGTGTA TGTGCTGAAT GATGGACTGA AGGCAGTGGC TGCGAATAGC
    CCGGAGTCCG GGGCTCTCCA GTGTCTCCAG CGCGCCTGCG ACGCGGAGAG TGCCATATTG
    ACCACAGTCA CCTTCGGACG ATTAGACTTT GGGGAGACCA CTGTGCTTGA TACTTTTTAC
    GATGCAGATA TTGCAGTTGT GGACATGAGT GATGTGTTCA GACAGCCCTC TTTGTTTTAT
    CACCTCGGGG TCCGTGAGAG CTTTGACATG GCCAACAATG TTATCCTCTA CCACGACATG
    GACCCCGACA CAGCACAGTC CCTTAAGGAC ATGGTGGCAC AGAAAAACAC AGCCTCTAGT
    GGGAACTACT ATTTCATCCC ATACCTGATG ACACCCAACA ACGAGTACTT GTGCTGTGAG
    AATGTGGCCC AGCGCAGAGC ATCAGAGTAC ATGCAGCCCA ACTGGGACAA CCTTTTAGGA
    CCTCTGTGTG TCCCTTTGGT GGACCGCTTC GTCAGCCTTC TCAAAGACAT TCATGTCACG
    TCTTGTGCTT CATACAAAGA TGCACTCCTG AATGACATCC GAAAAGCTCG AGATAAATAC
    CAGGGTGAGG AGCTGGCCAA AGAGCTTTCC CGTATCAAAC TCCGCATCGA CAACACTGAG
    GTTTTGACTC AAGATATTGT CATGAACCTG CTTTTCTCCT ACAGAGACAT ACAGGATTAT
    GATGCTATGG TTAAACTGGT GCAGACCATT GAGATGTTGC CCACCTGTGA CTTGGCCAAC
    CAGCCCATGA TTCAGTTCCA CTATGCTTTT GCGCTCAATA GACGAAACAG CCCTGGTGAT
    CGAGAGCAGG CGCTGCGTGT GATGTTGCAG GTTCTTCAAT CGTGTGAACA CCCTGCTCCT
    GACATGTTCT GCTTGTGTGG CCGCATATAT AAAGACATCT TTCTAGACTC AGACTGTAAA
    GACACCAAAA ACAGAGACAA CGCCATCCAG TGGTATAGAA AAGGTTTCGA GCTTCAGCCC
    ACGCTCTATT CAGGGATTAA TCTAGCAGTG CTGCTTATAG TAGCCGGGCA GCAGTTCGAG
    ACTTCCATAG AACTCAGGAA GATCGGTGTA AGGTTGAACA GTCTGCTGGG CCGTAAAGGC
    AGTCTGGAGA AGATGAATAA CTACTGGGAC GTTGGACAGT TTTTTACTGT CAGCATGCTG
    GCCAATGACA TCCCCAAAGC TGTGCAGGCC GCTGAGAAAC TCTTCAAGCT CAAACCACCT
    ATATGGTACC TGCGGTCAGT GGTCCAAAAC CTCCAGCTGA TTCAGCATTT CAAGAAGCAG
    AACACTGAAC ACTCGCCTCA AAGAGAGAGA CTCAACTTCT GGATGGACAT TATCGTGGAG
    GCCACGCAGG GAACCACCAA CGGCCTTCGT TTTCCGGTGT TGATTCTGGA GCCCACTAAA
    GTTTACCAGC CATCTTATGT ATCGATTAAC AGTGAAGCAG AAGAGAAGAA TGTGTCTATA
    TGGCACGTGT CGCCCGCTGA GACGAAGGGA ATACACGAGT GGAACTTCAC TGCGTCGTCC
    ATTAAAGGCA TCAGTATCTC AAAGTTTGAT GAGCGCTGCT GCTTTCTGTA CGTCCATGAT
    AATTCAGATG ACTTCCAGAT CTACTTCTCC ACAGAGGACC AGTGCGGCAG GTTCTGCTCA
    ATGGTGAAAG AGCTCATCAC AGATGGATCT GGAAATGCTG TTGAGCTGGA GGGCGAAGGA
    GACGGAGACA CCTTGGAGTA TGAGTATGAC TACAATGAGA ACGGTGACCG GGTGGTTCTG
    GGCAGGGGGA CCTATGGAGT TGTCTACGCA GGGAGAGACC TGAGCAATCA GGTGCGCATT
    GCCATCAAGG AGATCCCTGA GAGAGACAGC AGGTATTCCC AACCACTGCA TGAGGAAATC
    GCCCTTCACA AGTACCTGAA ACACAGAAAC ATAGTTCAGT ACCTGGGCTC AGTGTCAGAG
    GATGGTTATA TCAAGATCTT CATGGAGCAG GTCCCTGGAG GAAGTCTGTC AGCGTTGTTG
    CGTTCAAAGT GGGGTCCTTT GAAAGAAGCC ACCATAATCT TTTACACTCG GCAGATCCTG
    GAAGGCATTC GTTATTTGCA TGAAAACCAA ATAGTTCATC GGGATATCAA AGGAGACAAT
    GTTTTAGTCA ACACTTACAG TGGCGTGTTG AAAATCTCCG ACTTCGGCAC GTCAAAGAGA
    CTGGCTGGAG TCAATCCCTG CACTGAGACC TTCACTGGCA CCTTGCAGTA TATGGCCCCT
    GAGATCATCG ATAAGGGACC CAGAGGTTAT GGGGCCCCTG CTGACATCTG GTCTTTGGGC
    TGCACCATCA TTGAGATGGC GACAGGCAAA CCTCCTTTTC ATGAGCTGGG AGAGCCACAG
    GCCGCCATGT TTAAGGTGGG CATGTTTAAG ATCCATCCGG AGATCCCGGA GTCTCTTTCG
    TCGGAGGCAA AATCCTTCAT CCTGTCCAGC TTTGAGCCTG ATCCCAATAA ACGAGCAATG
    GCCGGCGACC TGCTCAAAGA CCCCTTCCTG CGGCAGAACA TCAAAGGCAA GAAGAACAAG
    ATCGCTTTCA AACCCTCAGT GTGGCAAGAC TACATCCGCA GTGTTTCTCT ACCCGTCCAG
    CTGCAGACAG AAGCCACAGG CAGCAGCAGC AGCGAGCCGG GCTCCGTCTC TCCAGACTGC
    GACTCCAAAC AAGATGTGTT TTTCGAGAAG AAGAAGCGTT CAAGCTCCGA AAACCTCATT
    AAACCACCCA GCTCAACCTT TCTCAGTGTT CCAGATGAGA GTCCGACAGC AGAAGACCGT
    AGCTCTCCAG CCTCCTCTGA GAACAGCGAC TCTGGTCTCT TCCTGCTCAA GAAAGACAGT
    GAAAGACGGG CCATTCTCTA CAAGGTCCTC AATGAAGACC AGGATAAAGT AACCTCCAAC
    CTCATGGAGA ACCACATCCA GGGTCAAGAG GAGCTGAAGT TGTCTGTGGA TCACATTAAA
    CAAATCATCT GCATCTTGAG AGACTTCATA CGCTGCCCTG AACGTCGTGT TATGGCCACC
    ACCATCTCAA AACTCAAACT GGACCTGGAC TTTGATAGCA CATCGATCAA CCAGATTCAA
    CTCGTTCTGT TCGGCTTTCA GGACTCTGTG AATAAAGTGT TACGGAACCA CCACATTAAA
    CCACACTGGA TGTTTGCCAT GGATAACATC ATCAGACGGG CTGTGCAGGC AGCTGTTACC
    ATTTTAATTC CAGAGCTGCA GACACATTTT GGTCCCGCAT CAGAGAGCGA GGGCGCAGAT
    AAAGACGCAG ATGAAGTAGA TGAAGAGGAC GATGCAGACT TTGGATCAGT AGCACGTACA
    ACACAGGAAG ATCAAGTTCT CACATCTGGA GTCAGCACAC TTAGTTCAGT CATATCCCAT
    GATGCGCAGC GGCTACAGCA CCCCCTAGGA GCACAGCTGG GACGACTCAA ACAGGAAACT
    TGCAGGCTTC TGGAGGAGCT GGTGCAGAAG GAGAGAGAAT ATCAGCAGGT TCTGAGACAA
    ACTCTCCAAC AGCGGGTACA TGACATCGAG CTTATCAGGA TCAGACAGCA GCCTGCTGCA
    GCAGAGATGC CCACTACACC CTCAGTATTT CACGTTACTG CACAACCCGA GCCTGACAAG
    AAGCTGACGG ATTGGCTGAA GGAGCAGGGA GCAGACAGCG AGACGATAGA CAAGTTTGTT
    GCAGAAGATT ATTCACTCAG CGACGTTCTC TGCGATATTA CTAAAGAAGA TCTTCGATGC
    CTGAGGCTTA GGGGTGGAAT GCTGTGTCGG ATCTGGAAAG CCATTCAACA ACATCGCAAC
    AAAGGACTTC GAAAGGTCAA AAACGACGAG GGAAATTCAG AAGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295755.1
    CDS776..4783
    Translation

    Target ORF information:

    RefSeq Version XM_009297480.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio mitogen-activated protein kinase kinase kinase 15 (map3k15), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009297480.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGATCA TGTCCAATCT ACCTACAGGA GAGGCTGGTG GCGACCACAC CAATGCGGTT 
    TCCATGTCTG CGGGCATGGA GAGAGCCGAT GTGACCAGCC CGAGTCCGGT GAGCAAACAG
    AGGTCCCTGC GGGCTGTGTA TGTGCTGAAT GATGGACTGA AGGCAGTGGC TGCGAATAGC
    CCGGAGTCCG GGGCTCTCCA GTGTCTCCAG CGCGCCTGCG ACGCGGAGAG TGCCATATTG
    ACCACAGTCA CCTTCGGACG ATTAGACTTT GGGGAGACCA CTGTGCTTGA TACTTTTTAC
    GATGCAGATA TTGCAGTTGT GGACATGAGT GATGTGTTCA GACAGCCCTC TTTGTTTTAT
    CACCTCGGGG TCCGTGAGAG CTTTGACATG GCCAACAATG TTATCCTCTA CCACGACATG
    GACCCCGACA CAGCACAGTC CCTTAAGGAC ATGGTGGCAC AGAAAAACAC AGCCTCTAGT
    GGGAACTACT ATTTCATCCC ATACCTGATG ACACCCAACA ACGAGTACTT GTGCTGTGAG
    AATGTGGCCC AGCGCAGAGC ATCAGAGTAC ATGCAGCCCA ACTGGGACAA CCTTTTAGGA
    CCTCTGTGTG TCCCTTTGGT GGACCGCTTC GTCAGCCTTC TCAAAGACAT TCATGTCACG
    TCTTGTGCTT CATACAAAGA TGCACTCCTG AATGACATCC GAAAAGCTCG AGATAAATAC
    CAGGGTGAGG AGCTGGCCAA AGAGCTTTCC CGTATCAAAC TCCGCATCGA CAACACTGAG
    GTTTTGACTC AAGATATTGT CATGAACCTG CTTTTCTCCT ACAGAGACAT ACAGGATTAT
    GATGCTATGG TTAAACTGGT GCAGACCATT GAGATGTTGC CCACCTGTGA CTTGGCCAAC
    CAGCCCATGA TTCAGTTCCA CTATGCTTTT GCGCTCAATA GACGAAACAG CCCTGGTGAT
    CGAGAGCAGG CGCTGCGTGT GATGTTGCAG GTTCTTCAAT CGTGTGAACA CCCTGCTCCT
    GACATGTTCT GCTTGTGTGG CCGCATATAT AAAGACATCT TTCTAGACTC AGACTGTAAA
    GACACCAAAA ACAGAGACAA CGCCATCCAG TGGTATAGAA AAGGTTTCGA GCTTCAGCCC
    ACGCTCTATT CAGGGATTAA TCTAGCAGTG CTGCTTATAG TAGCCGGGCA GCAGTTCGAG
    ACTTCCATAG AACTCAGGAA GATCGGTGTA AGGTTGAACA GTCTGCTGGG CCGTAAAGGC
    AGTCTGGAGA AGATGAATAA CTACTGGGAC GTTGGACAGT TTTTTACTGT CAGCATGCTG
    GCCAATGACA TCCCCAAAGC TGTGCAGGCC GCTGAGAAAC TCTTCAAGCT CAAACCACCT
    ATATGGTACC TGCGGTCAGT GGTCCAAAAC CTCCAGCTGA TTCAGCATTT CAAGAAGCAG
    AACACTGAAC ACTCGCCTCA AAGAGAGAGA CTCAACTTCT GGATGGACAT TATCGTGGAG
    GCCACGCAGG GAACCACCAA CGGCCTTCGT TTTCCGGTGT TGATTCTGGA GCCCACTAAA
    GTTTACCAGC CATCTTATGT ATCGATTAAC AGTGAAGCAG AAGAGAAGAA TGTGTCTATA
    TGGCACGTGT CGCCCGCTGA GACGAAGGGA ATACACGAGT GGAACTTCAC TGCGTCGTCC
    ATTAAAGGCA TCAGTATCTC AAAGTTTGAT GAGCGCTGCT GCTTTCTGTA CGTCCATGAT
    AATTCAGATG ACTTCCAGAT CTACTTCTCC ACAGAGGACC AGTGCGGCAG GTTCTGCTCA
    ATGGTGAAAG AGCTCATCAC AGATGGATCT GGAAATGCTG TTGAGCTGGA GGGCGAAGGA
    GACGGAGACA CCTTGGAGTA TGAGTATGAC TACAATGAGA ACGGTGACCG GGTGGTTCTG
    GGCAGGGGGA CCTATGGAGT TGTCTACGCA GGGAGAGACC TGAGCAATCA GGTGCGCATT
    GCCATCAAGG AGATCCCTGA GAGAGACAGC AGGTATTCCC AACCACTGCA TGAGGAAATC
    GCCCTTCACA AGTACCTGAA ACACAGAAAC ATAGTTCAGT ACCTGGGCTC AGTGTCAGAG
    GATGGTTATA TCAAGATCTT CATGGAGCAG GTCCCTGGAG GAAGTCTGTC AGCGTTGTTG
    CGTTCAAAGT GGGGTCCTTT GAAAGAAGCC ACCATAATCT TTTACACTCG GCAGATCCTG
    GAAGGCATTC GTTATTTGCA TGAAAACCAA ATAGTTCATC GGGATATCAA AGGAGACAAT
    GTTTTAGTCA ACACTTACAG TGGCGTGTTG AAAATCTCCG ACTTCGGCAC GTCAAAGAGA
    CTGGCTGGAG TCAATCCCTG CACTGAGACC TTCACTGGCA CCTTGCAGTA TATGGCCCCT
    GAGATCATCG ATAAGGGACC CAGAGGTTAT GGGGCCCCTG CTGACATCTG GTCTTTGGGC
    TGCACCATCA TTGAGATGGC GACAGGCAAA CCTCCTTTTC ATGAGCTGGG AGAGCCACAG
    GCCGCCATGT TTAAGGTGGG CATGTTTAAG ATCCATCCGG AGATCCCGGA GTCTCTTTCG
    TCGGAGGCAA AATCCTTCAT CCTGTCCAGC TTTGAGCCTG ATCCCAATAA ACGAGCAATG
    GCCGGCGACC TGCTCAAAGA CCCCTTCCTG CGGCAGAACA TCAAAGGCAA GAAGAACAAG
    ATCGCTTTCA AACCCTCAGT GTGGCAAGAC TACATCCGCA GTGTTTCTCT ACCCGTCCAG
    CTGCAGACAG AAGCCACAGG CAGCAGCAGC AGCGAGCCGG GCTCCGTCTC TCCAGACTGC
    GACTCCAAAC AAGATGTGTT TTTCGAGAAG AAGAAGCGTT CAAGCTCCGA AAACCTCATT
    AAACCACCCA GCTCAACCTT TCTCAGTGTT CCAGATGAGA GTCCGACAGC AGAAGACCGT
    AGCTCTCCAG CCTCCTCTGA GAACAGCGAC TCTGGTCTCT TCCTGCTCAA GAAAGACAGT
    GAAAGACGGG CCATTCTCTA CAAGGTCCTC AATGAAGACC AGGATAAAGT AACCTCCAAC
    CTCATGGAGA ACCACATCCA GGGTCAAGAG GAGCTGAAGT TGTCTGTGGA TCACATTAAA
    CAAATCATCT GCATCTTGAG AGACTTCATA CGCTGCCCTG AACGTCGTGT TATGGCCACC
    ACCATCTCAA AACTCAAACT GGACCTGGAC TTTGATAGCA CATCGATCAA CCAGATTCAA
    CTCGTTCTGT TCGGCTTTCA GGACTCTGTG AATAAAGTGT TACGGAACCA CCACATTAAA
    CCACACTGGA TGTTTGCCAT GGATAACATC ATCAGACGGG CTGTGCAGGC AGCTGTTACC
    ATTTTAATTC CAGAGCTGCA GACACATTTT GGTCCCGCAT CAGAGAGCGA GGGCGCAGAT
    AAAGACGCAG ATGAAGTAGA TGAAGAGGAC GATGCAGACT TTGGATCAGT AGCACGTACA
    ACACAGGAAG ATCAAGTTCT CACATCTGGA GTCAGCACAC TTAGTTCAGT CATATCCCAT
    GATGCGCAGC GGCTACAGCA CCCCCTAGGA GCACAGCTGG GACGACTCAA ACAGGAAACT
    TGCAGGCTTC TGGAGGAGCT GGTGCAGAAG GAGAGAGAAT ATCAGCAGGT TCTGAGACAA
    ACTCTCCAAC AGCGGGTACA TGACATCGAG CTTATCAGGA TCAGACAGCA GCCTGCTGCA
    GCAGAGATGC CCACTACACC CTCAGTATTT CACGTTACTG CACAACCCGA GCCTGACAAG
    AAGCTGACGG ATTGGCTGAA GGAGCAGGGA GCAGACAGCG AGACGATAGA CAAGTTTGTT
    GCAGAAGATT ATTCACTCAG CGACGTTCTC TGCGATATTA CTAAAGAAGA TCTTCGATGC
    CTGAGGCTTA GGGGTGGAAT GCTGTGTCGG ATCTGGAAAG CCATTCAACA ACATCGCAAC
    AAAGGACTTC GAAAGGTCAA AAACGACGAG GGAAATTCAG AAGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa28663
    Clone ID Related Accession (Same CDS sequence) XM_021473795.1 , XM_009297480.3 , XM_009297480.2
    Accession Version XM_009297480.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4008bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product mitogen-activated protein kinase kinase kinase 15 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297480.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGATCA TGTCCAATCT ACCTACAGGA GAGGCTGGTG GCGACCACAC CAATGCGGTT 
    TCCATGTCTG CGGGCATGGA GAGAGCCGAT GTGACCAGCC CGAGTCCGGT GAGCAAACAG
    AGGTCCCTGC GGGCTGTGTA TGTGCTGAAT GATGGACTGA AGGCAGTGGC TGCGAATAGC
    CCGGAGTCCG GGGCTCTCCA GTGTCTCCAG CGCGCCTGCG ACGCGGAGAG TGCCATATTG
    ACCACAGTCA CCTTCGGACG ATTAGACTTT GGGGAGACCA CTGTGCTTGA TACTTTTTAC
    GATGCAGATA TTGCAGTTGT GGACATGAGT GATGTGTTCA GACAGCCCTC TTTGTTTTAT
    CACCTCGGGG TCCGTGAGAG CTTTGACATG GCCAACAATG TTATCCTCTA CCACGACATG
    GACCCCGACA CAGCACAGTC CCTTAAGGAC ATGGTGGCAC AGAAAAACAC AGCCTCTAGT
    GGGAACTACT ATTTCATCCC ATACCTGATG ACACCCAACA ACGAGTACTT GTGCTGTGAG
    AATGTGGCCC AGCGCAGAGC ATCAGAGTAC ATGCAGCCCA ACTGGGACAA CCTTTTAGGA
    CCTCTGTGTG TCCCTTTGGT GGACCGCTTC GTCAGCCTTC TCAAAGACAT TCATGTCACG
    TCTTGTGCTT CATACAAAGA TGCACTCCTG AATGACATCC GAAAAGCTCG AGATAAATAC
    CAGGGTGAGG AGCTGGCCAA AGAGCTTTCC CGTATCAAAC TCCGCATCGA CAACACTGAG
    GTTTTGACTC AAGATATTGT CATGAACCTG CTTTTCTCCT ACAGAGACAT ACAGGATTAT
    GATGCTATGG TTAAACTGGT GCAGACCATT GAGATGTTGC CCACCTGTGA CTTGGCCAAC
    CAGCCCATGA TTCAGTTCCA CTATGCTTTT GCGCTCAATA GACGAAACAG CCCTGGTGAT
    CGAGAGCAGG CGCTGCGTGT GATGTTGCAG GTTCTTCAAT CGTGTGAACA CCCTGCTCCT
    GACATGTTCT GCTTGTGTGG CCGCATATAT AAAGACATCT TTCTAGACTC AGACTGTAAA
    GACACCAAAA ACAGAGACAA CGCCATCCAG TGGTATAGAA AAGGTTTCGA GCTTCAGCCC
    ACGCTCTATT CAGGGATTAA TCTAGCAGTG CTGCTTATAG TAGCCGGGCA GCAGTTCGAG
    ACTTCCATAG AACTCAGGAA GATCGGTGTA AGGTTGAACA GTCTGCTGGG CCGTAAAGGC
    AGTCTGGAGA AGATGAATAA CTACTGGGAC GTTGGACAGT TTTTTACTGT CAGCATGCTG
    GCCAATGACA TCCCCAAAGC TGTGCAGGCC GCTGAGAAAC TCTTCAAGCT CAAACCACCT
    ATATGGTACC TGCGGTCAGT GGTCCAAAAC CTCCAGCTGA TTCAGCATTT CAAGAAGCAG
    AACACTGAAC ACTCGCCTCA AAGAGAGAGA CTCAACTTCT GGATGGACAT TATCGTGGAG
    GCCACGCAGG GAACCACCAA CGGCCTTCGT TTTCCGGTGT TGATTCTGGA GCCCACTAAA
    GTTTACCAGC CATCTTATGT ATCGATTAAC AGTGAAGCAG AAGAGAAGAA TGTGTCTATA
    TGGCACGTGT CGCCCGCTGA GACGAAGGGA ATACACGAGT GGAACTTCAC TGCGTCGTCC
    ATTAAAGGCA TCAGTATCTC AAAGTTTGAT GAGCGCTGCT GCTTTCTGTA CGTCCATGAT
    AATTCAGATG ACTTCCAGAT CTACTTCTCC ACAGAGGACC AGTGCGGCAG GTTCTGCTCA
    ATGGTGAAAG AGCTCATCAC AGATGGATCT GGAAATGCTG TTGAGCTGGA GGGCGAAGGA
    GACGGAGACA CCTTGGAGTA TGAGTATGAC TACAATGAGA ACGGTGACCG GGTGGTTCTG
    GGCAGGGGGA CCTATGGAGT TGTCTACGCA GGGAGAGACC TGAGCAATCA GGTGCGCATT
    GCCATCAAGG AGATCCCTGA GAGAGACAGC AGGTATTCCC AACCACTGCA TGAGGAAATC
    GCCCTTCACA AGTACCTGAA ACACAGAAAC ATAGTTCAGT ACCTGGGCTC AGTGTCAGAG
    GATGGTTATA TCAAGATCTT CATGGAGCAG GTCCCTGGAG GAAGTCTGTC AGCGTTGTTG
    CGTTCAAAGT GGGGTCCTTT GAAAGAAGCC ACCATAATCT TTTACACTCG GCAGATCCTG
    GAAGGCATTC GTTATTTGCA TGAAAACCAA ATAGTTCATC GGGATATCAA AGGAGACAAT
    GTTTTAGTCA ACACTTACAG TGGCGTGTTG AAAATCTCCG ACTTCGGCAC GTCAAAGAGA
    CTGGCTGGAG TCAATCCCTG CACTGAGACC TTCACTGGCA CCTTGCAGTA TATGGCCCCT
    GAGATCATCG ATAAGGGACC CAGAGGTTAT GGGGCCCCTG CTGACATCTG GTCTTTGGGC
    TGCACCATCA TTGAGATGGC GACAGGCAAA CCTCCTTTTC ATGAGCTGGG AGAGCCACAG
    GCCGCCATGT TTAAGGTGGG CATGTTTAAG ATCCATCCGG AGATCCCGGA GTCTCTTTCG
    TCGGAGGCAA AATCCTTCAT CCTGTCCAGC TTTGAGCCTG ATCCCAATAA ACGAGCAATG
    GCCGGCGACC TGCTCAAAGA CCCCTTCCTG CGGCAGAACA TCAAAGGCAA GAAGAACAAG
    ATCGCTTTCA AACCCTCAGT GTGGCAAGAC TACATCCGCA GTGTTTCTCT ACCCGTCCAG
    CTGCAGACAG AAGCCACAGG CAGCAGCAGC AGCGAGCCGG GCTCCGTCTC TCCAGACTGC
    GACTCCAAAC AAGATGTGTT TTTCGAGAAG AAGAAGCGTT CAAGCTCCGA AAACCTCATT
    AAACCACCCA GCTCAACCTT TCTCAGTGTT CCAGATGAGA GTCCGACAGC AGAAGACCGT
    AGCTCTCCAG CCTCCTCTGA GAACAGCGAC TCTGGTCTCT TCCTGCTCAA GAAAGACAGT
    GAAAGACGGG CCATTCTCTA CAAGGTCCTC AATGAAGACC AGGATAAAGT AACCTCCAAC
    CTCATGGAGA ACCACATCCA GGGTCAAGAG GAGCTGAAGT TGTCTGTGGA TCACATTAAA
    CAAATCATCT GCATCTTGAG AGACTTCATA CGCTGCCCTG AACGTCGTGT TATGGCCACC
    ACCATCTCAA AACTCAAACT GGACCTGGAC TTTGATAGCA CATCGATCAA CCAGATTCAA
    CTCGTTCTGT TCGGCTTTCA GGACTCTGTG AATAAAGTGT TACGGAACCA CCACATTAAA
    CCACACTGGA TGTTTGCCAT GGATAACATC ATCAGACGGG CTGTGCAGGC AGCTGTTACC
    ATTTTAATTC CAGAGCTGCA GACACATTTT GGTCCCGCAT CAGAGAGCGA GGGCGCAGAT
    AAAGACGCAG ATGAAGTAGA TGAAGAGGAC GATGCAGACT TTGGATCAGT AGCACGTACA
    ACACAGGAAG ATCAAGTTCT CACATCTGGA GTCAGCACAC TTAGTTCAGT CATATCCCAT
    GATGCGCAGC GGCTACAGCA CCCCCTAGGA GCACAGCTGG GACGACTCAA ACAGGAAACT
    TGCAGGCTTC TGGAGGAGCT GGTGCAGAAG GAGAGAGAAT ATCAGCAGGT TCTGAGACAA
    ACTCTCCAAC AGCGGGTACA TGACATCGAG CTTATCAGGA TCAGACAGCA GCCTGCTGCA
    GCAGAGATGC CCACTACACC CTCAGTATTT CACGTTACTG CACAACCCGA GCCTGACAAG
    AAGCTGACGG ATTGGCTGAA GGAGCAGGGA GCAGACAGCG AGACGATAGA CAAGTTTGTT
    GCAGAAGATT ATTCACTCAG CGACGTTCTC TGCGATATTA CTAAAGAAGA TCTTCGATGC
    CTGAGGCTTA GGGGTGGAAT GCTGTGTCGG ATCTGGAAAG CCATTCAACA ACATCGCAAC
    AAAGGACTTC GAAAGGTCAA AAACGACGAG GGAAATTCAG AAGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295755.1
    CDS683..4690
    Translation

    Target ORF information:

    RefSeq Version XM_009297480.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio mitogen-activated protein kinase kinase kinase 15 (map3k15), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009297480.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGATCA TGTCCAATCT ACCTACAGGA GAGGCTGGTG GCGACCACAC CAATGCGGTT 
    TCCATGTCTG CGGGCATGGA GAGAGCCGAT GTGACCAGCC CGAGTCCGGT GAGCAAACAG
    AGGTCCCTGC GGGCTGTGTA TGTGCTGAAT GATGGACTGA AGGCAGTGGC TGCGAATAGC
    CCGGAGTCCG GGGCTCTCCA GTGTCTCCAG CGCGCCTGCG ACGCGGAGAG TGCCATATTG
    ACCACAGTCA CCTTCGGACG ATTAGACTTT GGGGAGACCA CTGTGCTTGA TACTTTTTAC
    GATGCAGATA TTGCAGTTGT GGACATGAGT GATGTGTTCA GACAGCCCTC TTTGTTTTAT
    CACCTCGGGG TCCGTGAGAG CTTTGACATG GCCAACAATG TTATCCTCTA CCACGACATG
    GACCCCGACA CAGCACAGTC CCTTAAGGAC ATGGTGGCAC AGAAAAACAC AGCCTCTAGT
    GGGAACTACT ATTTCATCCC ATACCTGATG ACACCCAACA ACGAGTACTT GTGCTGTGAG
    AATGTGGCCC AGCGCAGAGC ATCAGAGTAC ATGCAGCCCA ACTGGGACAA CCTTTTAGGA
    CCTCTGTGTG TCCCTTTGGT GGACCGCTTC GTCAGCCTTC TCAAAGACAT TCATGTCACG
    TCTTGTGCTT CATACAAAGA TGCACTCCTG AATGACATCC GAAAAGCTCG AGATAAATAC
    CAGGGTGAGG AGCTGGCCAA AGAGCTTTCC CGTATCAAAC TCCGCATCGA CAACACTGAG
    GTTTTGACTC AAGATATTGT CATGAACCTG CTTTTCTCCT ACAGAGACAT ACAGGATTAT
    GATGCTATGG TTAAACTGGT GCAGACCATT GAGATGTTGC CCACCTGTGA CTTGGCCAAC
    CAGCCCATGA TTCAGTTCCA CTATGCTTTT GCGCTCAATA GACGAAACAG CCCTGGTGAT
    CGAGAGCAGG CGCTGCGTGT GATGTTGCAG GTTCTTCAAT CGTGTGAACA CCCTGCTCCT
    GACATGTTCT GCTTGTGTGG CCGCATATAT AAAGACATCT TTCTAGACTC AGACTGTAAA
    GACACCAAAA ACAGAGACAA CGCCATCCAG TGGTATAGAA AAGGTTTCGA GCTTCAGCCC
    ACGCTCTATT CAGGGATTAA TCTAGCAGTG CTGCTTATAG TAGCCGGGCA GCAGTTCGAG
    ACTTCCATAG AACTCAGGAA GATCGGTGTA AGGTTGAACA GTCTGCTGGG CCGTAAAGGC
    AGTCTGGAGA AGATGAATAA CTACTGGGAC GTTGGACAGT TTTTTACTGT CAGCATGCTG
    GCCAATGACA TCCCCAAAGC TGTGCAGGCC GCTGAGAAAC TCTTCAAGCT CAAACCACCT
    ATATGGTACC TGCGGTCAGT GGTCCAAAAC CTCCAGCTGA TTCAGCATTT CAAGAAGCAG
    AACACTGAAC ACTCGCCTCA AAGAGAGAGA CTCAACTTCT GGATGGACAT TATCGTGGAG
    GCCACGCAGG GAACCACCAA CGGCCTTCGT TTTCCGGTGT TGATTCTGGA GCCCACTAAA
    GTTTACCAGC CATCTTATGT ATCGATTAAC AGTGAAGCAG AAGAGAAGAA TGTGTCTATA
    TGGCACGTGT CGCCCGCTGA GACGAAGGGA ATACACGAGT GGAACTTCAC TGCGTCGTCC
    ATTAAAGGCA TCAGTATCTC AAAGTTTGAT GAGCGCTGCT GCTTTCTGTA CGTCCATGAT
    AATTCAGATG ACTTCCAGAT CTACTTCTCC ACAGAGGACC AGTGCGGCAG GTTCTGCTCA
    ATGGTGAAAG AGCTCATCAC AGATGGATCT GGAAATGCTG TTGAGCTGGA GGGCGAAGGA
    GACGGAGACA CCTTGGAGTA TGAGTATGAC TACAATGAGA ACGGTGACCG GGTGGTTCTG
    GGCAGGGGGA CCTATGGAGT TGTCTACGCA GGGAGAGACC TGAGCAATCA GGTGCGCATT
    GCCATCAAGG AGATCCCTGA GAGAGACAGC AGGTATTCCC AACCACTGCA TGAGGAAATC
    GCCCTTCACA AGTACCTGAA ACACAGAAAC ATAGTTCAGT ACCTGGGCTC AGTGTCAGAG
    GATGGTTATA TCAAGATCTT CATGGAGCAG GTCCCTGGAG GAAGTCTGTC AGCGTTGTTG
    CGTTCAAAGT GGGGTCCTTT GAAAGAAGCC ACCATAATCT TTTACACTCG GCAGATCCTG
    GAAGGCATTC GTTATTTGCA TGAAAACCAA ATAGTTCATC GGGATATCAA AGGAGACAAT
    GTTTTAGTCA ACACTTACAG TGGCGTGTTG AAAATCTCCG ACTTCGGCAC GTCAAAGAGA
    CTGGCTGGAG TCAATCCCTG CACTGAGACC TTCACTGGCA CCTTGCAGTA TATGGCCCCT
    GAGATCATCG ATAAGGGACC CAGAGGTTAT GGGGCCCCTG CTGACATCTG GTCTTTGGGC
    TGCACCATCA TTGAGATGGC GACAGGCAAA CCTCCTTTTC ATGAGCTGGG AGAGCCACAG
    GCCGCCATGT TTAAGGTGGG CATGTTTAAG ATCCATCCGG AGATCCCGGA GTCTCTTTCG
    TCGGAGGCAA AATCCTTCAT CCTGTCCAGC TTTGAGCCTG ATCCCAATAA ACGAGCAATG
    GCCGGCGACC TGCTCAAAGA CCCCTTCCTG CGGCAGAACA TCAAAGGCAA GAAGAACAAG
    ATCGCTTTCA AACCCTCAGT GTGGCAAGAC TACATCCGCA GTGTTTCTCT ACCCGTCCAG
    CTGCAGACAG AAGCCACAGG CAGCAGCAGC AGCGAGCCGG GCTCCGTCTC TCCAGACTGC
    GACTCCAAAC AAGATGTGTT TTTCGAGAAG AAGAAGCGTT CAAGCTCCGA AAACCTCATT
    AAACCACCCA GCTCAACCTT TCTCAGTGTT CCAGATGAGA GTCCGACAGC AGAAGACCGT
    AGCTCTCCAG CCTCCTCTGA GAACAGCGAC TCTGGTCTCT TCCTGCTCAA GAAAGACAGT
    GAAAGACGGG CCATTCTCTA CAAGGTCCTC AATGAAGACC AGGATAAAGT AACCTCCAAC
    CTCATGGAGA ACCACATCCA GGGTCAAGAG GAGCTGAAGT TGTCTGTGGA TCACATTAAA
    CAAATCATCT GCATCTTGAG AGACTTCATA CGCTGCCCTG AACGTCGTGT TATGGCCACC
    ACCATCTCAA AACTCAAACT GGACCTGGAC TTTGATAGCA CATCGATCAA CCAGATTCAA
    CTCGTTCTGT TCGGCTTTCA GGACTCTGTG AATAAAGTGT TACGGAACCA CCACATTAAA
    CCACACTGGA TGTTTGCCAT GGATAACATC ATCAGACGGG CTGTGCAGGC AGCTGTTACC
    ATTTTAATTC CAGAGCTGCA GACACATTTT GGTCCCGCAT CAGAGAGCGA GGGCGCAGAT
    AAAGACGCAG ATGAAGTAGA TGAAGAGGAC GATGCAGACT TTGGATCAGT AGCACGTACA
    ACACAGGAAG ATCAAGTTCT CACATCTGGA GTCAGCACAC TTAGTTCAGT CATATCCCAT
    GATGCGCAGC GGCTACAGCA CCCCCTAGGA GCACAGCTGG GACGACTCAA ACAGGAAACT
    TGCAGGCTTC TGGAGGAGCT GGTGCAGAAG GAGAGAGAAT ATCAGCAGGT TCTGAGACAA
    ACTCTCCAAC AGCGGGTACA TGACATCGAG CTTATCAGGA TCAGACAGCA GCCTGCTGCA
    GCAGAGATGC CCACTACACC CTCAGTATTT CACGTTACTG CACAACCCGA GCCTGACAAG
    AAGCTGACGG ATTGGCTGAA GGAGCAGGGA GCAGACAGCG AGACGATAGA CAAGTTTGTT
    GCAGAAGATT ATTCACTCAG CGACGTTCTC TGCGATATTA CTAAAGAAGA TCTTCGATGC
    CTGAGGCTTA GGGGTGGAAT GCTGTGTCGG ATCTGGAAAG CCATTCAACA ACATCGCAAC
    AAAGGACTTC GAAAGGTCAA AAACGACGAG GGAAATTCAG AAGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa49942
    Clone ID Related Accession (Same CDS sequence) XM_009297481.2
    Accession Version XM_009297481.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4005bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product mitogen-activated protein kinase kinase kinase 15 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297481.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGATCA TGTCCAATCT ACCTACAGGA GAGGCTGGTG GCGACCACAC CAATGCGGTT 
    TCCATGTCTG CGGGCATGGA GAGAGCCGAT GTGACCAGCC CGAGTCCGGT GAGCAAACAG
    AGGTCCCTGC GGGCTGTGTA TGTGCTGAAT GATGGACTGA AGGCAGTGGC TGCGAATAGC
    CCGGAGTCCG GGGCTCTCCA GTGTCTCCAG CGCGCCTGCG ACGCGGAGAG TGCCATATTG
    ACCACAGTCA CCTTCGGACG ATTAGACTTT GGGGAGACCA CTGTGCTTGA TACTTTTTAC
    GATGCAGATA TTGCAGTTGT GGACATGAGT GATGTGTTCA GACAGCCCTC TTTGTTTTAT
    CACCTCGGGG TCCGTGAGAG CTTTGACATG GCCAACAATG TTATCCTCTA CCACGACATG
    GACCCCGACA CAGCACAGTC CCTTAAGGAC ATGGTGGCAC AGAAAAACAC AGCCTCTAGT
    GGGAACTACT ATTTCATCCC ATACCTGATG ACACCCAACA ACGAGTACTT GTGCTGTGAG
    AATGTGGCCC AGCGCAGAGC ATCAGAGTAC ATGCAGCCCA ACTGGGACAA CCTTTTAGGA
    CCTCTGTGTG TCCCTTTGGT GGACCGCTTC GTCAGCCTTC TCAAAGACAT TCATGTCACG
    TCTTGTGCTT CATACAAAGA TGCACTCCTG AATGACATCC GAAAAGCTCG AGATAAATAC
    CAGGGTGAGG AGCTGGCCAA AGAGCTTTCC CGTATCAAAC TCCGCATCGA CAACACTGAG
    GTTTTGACTC AAGATATTGT CATGAACCTG CTTTTCTCCT ACAGAGACAT ACAGGATTAT
    GATGCTATGG TTAAACTGGT GCAGACCATT GAGATGTTGC CCACCTGTGA CTTGGCCAAC
    CAGCCCATGA TTCAGTTCCA CTATGCTTTT GCGCTCAATA GACGAAACAG CCCTGGTGAT
    CGAGAGCAGG CGCTGCGTGT GATGTTGCAG GTTCTTCAAT CGTGTGAACA CCCTGCTCCT
    GACATGTTCT GCTTGTGTGG CCGCATATAT AAAGACATCT TTCTAGACTC AGACTGTAAA
    GACACCAAAA ACAGAGACAA CGCCATCCAG TGGTATAGAA AAGGTTTCGA GCTTCAGCCC
    ACGCTCTATT CAGGGATTAA TCTAGCAGTG CTGCTTATAG TAGCCGGGCA GCAGTTCGAG
    ACTTCCATAG AACTCAGGAA GATCGGTGTA AGGTTGAACA GTCTGCTGGG CCGTAAAGGC
    AGTCTGGAGA AGATGAATAA CTACTGGGAC GTTGGACAGT TTTTTACTGT CAGCATGCTG
    GCCAATGACA TCCCCAAAGC TGTGCAGGCC GCTGAGAAAC TCTTCAAGCT CAAACCACCT
    ATATGGTACC TGCGGTCAGT GGTCCAAAAC CTCCAGCTGA TTCAGCATTT CAAGAAGCAG
    AACACTGAAC ACTCGCCTCA AAGAGAGAGA CTCAACTTCT GGATGGACAT TATCGTGGAG
    GCCACGCAGG GAACCACCAA CGGCCTTCGT TTTCCGGTGT TGATTCTGGA GCCCACTAAA
    GTTTACCAGC CATCTTATGT ATCGATTAAC AGTGAAGCAG AAGAGAAGAA TGTGTCTATA
    TGGCACGTGT CGCCCGCTGA GACGAAGGGA ATACACGAGT GGAACTTCAC TGCGTCGTCC
    ATTAAAGGCA TCAGTATCTC AAAGTTTGAT GAGCGCTGCT GCTTTCTGTA CGTCCATGAT
    AATTCAGATG ACTTCCAGAT CTACTTCTCC ACAGAGGACC AGTGCGGCAG GTTCTGCTCA
    ATGGTGAAAG AGCTCATCAC AGATGGATCT GGAAATGCTG TTGAGCTGGA GGGCGAAGGA
    GACGGAGACA CCTTGGAGTA TGAGTATGAC TACAATGAGA ACGGTGACCG GGTGGTTCTG
    GGCAGGGGGA CCTATGGAGT TGTCTACGCA GGGAGAGACC TGAGCAATCA GGTGCGCATT
    GCCATCAAGG AGATCCCTGA GAGAGACAGC AGGTATTCCC AACCACTGCA TGAGGAAATC
    GCCCTTCACA AGTACCTGAA ACACAGAAAC ATAGTTCAGT ACCTGGGCTC AGTGTCAGAG
    GATGGTTATA TCAAGATCTT CATGGAGCAG GTCCCTGGAG GAAGTCTGTC AGCGTTGTTG
    CGTTCAAAGT GGGGTCCTTT GAAAGAAGCC ACCATAATCT TTTACACTCG GCAGATCCTG
    GAAGGCATTC GTTATTTGCA TGAAAACCAA ATAGTTCATC GGGATATCAA AGGAGACAAT
    GTTTTAGTCA ACACTTACAG TGGCGTGTTG AAAATCTCCG ACTTCGGCAC GTCAAAGAGA
    CTGGCTGGAG TCAATCCCTG CACTGAGACC TTCACTGGCA CCTTGCAGTA TATGGCCCCT
    GAGATCATCG ATAAGGGACC CAGAGGTTAT GGGGCCCCTG CTGACATCTG GTCTTTGGGC
    TGCACCATCA TTGAGATGGC GACAGGCAAA CCTCCTTTTC ATGAGCTGGG AGAGCCACAG
    GCCGCCATGT TTAAGGTGGG CATGTTTAAG ATCCATCCGG AGATCCCGGA GTCTCTTTCG
    TCGGAGGCAA AATCCTTCAT CCTGTCCAGC TTTGAGCCTG ATCCCAATAA ACGAGCAATG
    GCCGGCGACC TGCTCAAAGA CCCCTTCCTG CGGCAGAACA TCAAAGGCAA GAAGAACAAG
    ATCGCTTTCA AACCCTCAGT GTGGCAAGAC TACATCCGCA GTGTTTCTCT ACCCGTCCAG
    CTGCAGACAG AAGCCACAGG CAGCAGCAGC AGCGAGCCGG GCTCCGTCTC TCCAGACTGC
    GACTCCAAAC AAGATGTGTT TTTCGAGAAG AAGAAGCGTT CAAGCTCCGA AAACCTCATT
    AAACCACCCA GCTCAACCTT TCTCAGTGTT CCAGATGAGA GTCCGACAGC AGAAGACCGT
    AGCTCTCCAG CCTCCTCTGA GAACAGCGAC TCTGGTCTCT TCCTGCTCAA GAAAGACAGT
    GAAAGACGGG CCATTCTCTA CAAGGTCCTC AATGAAGACC AGGATAAAGT AACCTCCAAC
    CTCATGGAGA ACCACATCCA GGGTCAAGAG GAGCTGAAGT TGTCTGTGGA TCACATTAAA
    CAAATCATCT GCATCTTGAG AGACTTCATA CGCTGCCCTG AACGTCGTGT TATGGCCACC
    ACCATCTCAA AACTCAAACT GGACCTGGAC TTTGATAGCA CATCGATCAA CCAGATTCAA
    CTCGTTCTGT TCGGCTTTCA GGACTCTGTG AATAAAGTGT TACGGAACCA CCACATTAAA
    CCACACTGGA TGTTTGCCAT GGATAACATC ATCAGACGGG CTGTGCAGGC AGCTGTTACC
    ATTTTAATTC CAGAGCTGCA GACACATTTT GGTCCCGCAT CAGAGAGCGA GGGCGCAGAT
    AAAGACGCAG ATGAAGTAGA TGAAGAGGAC GATGCAGACT TTGGATCAGT AGCACGTACA
    ACACAGGAAG ATCAAGTTCT CACATCTGGA GTCAGCACAC TTAGTTCAGT CATATCCCAT
    GATGCGCAGC GGCTACAGCA CCCCCTAGGA GCACAGCTGG GACGACTCAA ACAGGAAACT
    TGCAGGCTTC TGGAGGAGCT GGTGCAGAAG GAGAGAGAAT ATCAGCAGGT TCTGAGACAA
    ACTCTCCAAC AGCGGGTACA TGACATCGAG CTTATCAGGA TCAGACAGCA GCCTGCTGCA
    GAGATGCCCA CTACACCCTC AGTATTTCAC GTTACTGCAC AACCCGAGCC TGACAAGAAG
    CTGACGGATT GGCTGAAGGA GCAGGGAGCA GACAGCGAGA CGATAGACAA GTTTGTTGCA
    GAAGATTATT CACTCAGCGA CGTTCTCTGC GATATTACTA AAGAAGATCT TCGATGCCTG
    AGGCTTAGGG GTGGAATGCT GTGTCGGATC TGGAAAGCCA TTCAACAACA TCGCAACAAA
    GGACTTCGAA AGGTCAAAAA CGACGAGGGA AATTCAGAAG GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295756.1
    CDS682..4686
    Translation

    Target ORF information:

    RefSeq Version XM_009297481.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio mitogen-activated protein kinase kinase kinase 15 (map3k15), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009297481.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGATCA TGTCCAATCT ACCTACAGGA GAGGCTGGTG GCGACCACAC CAATGCGGTT 
    TCCATGTCTG CGGGCATGGA GAGAGCCGAT GTGACCAGCC CGAGTCCGGT GAGCAAACAG
    AGGTCCCTGC GGGCTGTGTA TGTGCTGAAT GATGGACTGA AGGCAGTGGC TGCGAATAGC
    CCGGAGTCCG GGGCTCTCCA GTGTCTCCAG CGCGCCTGCG ACGCGGAGAG TGCCATATTG
    ACCACAGTCA CCTTCGGACG ATTAGACTTT GGGGAGACCA CTGTGCTTGA TACTTTTTAC
    GATGCAGATA TTGCAGTTGT GGACATGAGT GATGTGTTCA GACAGCCCTC TTTGTTTTAT
    CACCTCGGGG TCCGTGAGAG CTTTGACATG GCCAACAATG TTATCCTCTA CCACGACATG
    GACCCCGACA CAGCACAGTC CCTTAAGGAC ATGGTGGCAC AGAAAAACAC AGCCTCTAGT
    GGGAACTACT ATTTCATCCC ATACCTGATG ACACCCAACA ACGAGTACTT GTGCTGTGAG
    AATGTGGCCC AGCGCAGAGC ATCAGAGTAC ATGCAGCCCA ACTGGGACAA CCTTTTAGGA
    CCTCTGTGTG TCCCTTTGGT GGACCGCTTC GTCAGCCTTC TCAAAGACAT TCATGTCACG
    TCTTGTGCTT CATACAAAGA TGCACTCCTG AATGACATCC GAAAAGCTCG AGATAAATAC
    CAGGGTGAGG AGCTGGCCAA AGAGCTTTCC CGTATCAAAC TCCGCATCGA CAACACTGAG
    GTTTTGACTC AAGATATTGT CATGAACCTG CTTTTCTCCT ACAGAGACAT ACAGGATTAT
    GATGCTATGG TTAAACTGGT GCAGACCATT GAGATGTTGC CCACCTGTGA CTTGGCCAAC
    CAGCCCATGA TTCAGTTCCA CTATGCTTTT GCGCTCAATA GACGAAACAG CCCTGGTGAT
    CGAGAGCAGG CGCTGCGTGT GATGTTGCAG GTTCTTCAAT CGTGTGAACA CCCTGCTCCT
    GACATGTTCT GCTTGTGTGG CCGCATATAT AAAGACATCT TTCTAGACTC AGACTGTAAA
    GACACCAAAA ACAGAGACAA CGCCATCCAG TGGTATAGAA AAGGTTTCGA GCTTCAGCCC
    ACGCTCTATT CAGGGATTAA TCTAGCAGTG CTGCTTATAG TAGCCGGGCA GCAGTTCGAG
    ACTTCCATAG AACTCAGGAA GATCGGTGTA AGGTTGAACA GTCTGCTGGG CCGTAAAGGC
    AGTCTGGAGA AGATGAATAA CTACTGGGAC GTTGGACAGT TTTTTACTGT CAGCATGCTG
    GCCAATGACA TCCCCAAAGC TGTGCAGGCC GCTGAGAAAC TCTTCAAGCT CAAACCACCT
    ATATGGTACC TGCGGTCAGT GGTCCAAAAC CTCCAGCTGA TTCAGCATTT CAAGAAGCAG
    AACACTGAAC ACTCGCCTCA AAGAGAGAGA CTCAACTTCT GGATGGACAT TATCGTGGAG
    GCCACGCAGG GAACCACCAA CGGCCTTCGT TTTCCGGTGT TGATTCTGGA GCCCACTAAA
    GTTTACCAGC CATCTTATGT ATCGATTAAC AGTGAAGCAG AAGAGAAGAA TGTGTCTATA
    TGGCACGTGT CGCCCGCTGA GACGAAGGGA ATACACGAGT GGAACTTCAC TGCGTCGTCC
    ATTAAAGGCA TCAGTATCTC AAAGTTTGAT GAGCGCTGCT GCTTTCTGTA CGTCCATGAT
    AATTCAGATG ACTTCCAGAT CTACTTCTCC ACAGAGGACC AGTGCGGCAG GTTCTGCTCA
    ATGGTGAAAG AGCTCATCAC AGATGGATCT GGAAATGCTG TTGAGCTGGA GGGCGAAGGA
    GACGGAGACA CCTTGGAGTA TGAGTATGAC TACAATGAGA ACGGTGACCG GGTGGTTCTG
    GGCAGGGGGA CCTATGGAGT TGTCTACGCA GGGAGAGACC TGAGCAATCA GGTGCGCATT
    GCCATCAAGG AGATCCCTGA GAGAGACAGC AGGTATTCCC AACCACTGCA TGAGGAAATC
    GCCCTTCACA AGTACCTGAA ACACAGAAAC ATAGTTCAGT ACCTGGGCTC AGTGTCAGAG
    GATGGTTATA TCAAGATCTT CATGGAGCAG GTCCCTGGAG GAAGTCTGTC AGCGTTGTTG
    CGTTCAAAGT GGGGTCCTTT GAAAGAAGCC ACCATAATCT TTTACACTCG GCAGATCCTG
    GAAGGCATTC GTTATTTGCA TGAAAACCAA ATAGTTCATC GGGATATCAA AGGAGACAAT
    GTTTTAGTCA ACACTTACAG TGGCGTGTTG AAAATCTCCG ACTTCGGCAC GTCAAAGAGA
    CTGGCTGGAG TCAATCCCTG CACTGAGACC TTCACTGGCA CCTTGCAGTA TATGGCCCCT
    GAGATCATCG ATAAGGGACC CAGAGGTTAT GGGGCCCCTG CTGACATCTG GTCTTTGGGC
    TGCACCATCA TTGAGATGGC GACAGGCAAA CCTCCTTTTC ATGAGCTGGG AGAGCCACAG
    GCCGCCATGT TTAAGGTGGG CATGTTTAAG ATCCATCCGG AGATCCCGGA GTCTCTTTCG
    TCGGAGGCAA AATCCTTCAT CCTGTCCAGC TTTGAGCCTG ATCCCAATAA ACGAGCAATG
    GCCGGCGACC TGCTCAAAGA CCCCTTCCTG CGGCAGAACA TCAAAGGCAA GAAGAACAAG
    ATCGCTTTCA AACCCTCAGT GTGGCAAGAC TACATCCGCA GTGTTTCTCT ACCCGTCCAG
    CTGCAGACAG AAGCCACAGG CAGCAGCAGC AGCGAGCCGG GCTCCGTCTC TCCAGACTGC
    GACTCCAAAC AAGATGTGTT TTTCGAGAAG AAGAAGCGTT CAAGCTCCGA AAACCTCATT
    AAACCACCCA GCTCAACCTT TCTCAGTGTT CCAGATGAGA GTCCGACAGC AGAAGACCGT
    AGCTCTCCAG CCTCCTCTGA GAACAGCGAC TCTGGTCTCT TCCTGCTCAA GAAAGACAGT
    GAAAGACGGG CCATTCTCTA CAAGGTCCTC AATGAAGACC AGGATAAAGT AACCTCCAAC
    CTCATGGAGA ACCACATCCA GGGTCAAGAG GAGCTGAAGT TGTCTGTGGA TCACATTAAA
    CAAATCATCT GCATCTTGAG AGACTTCATA CGCTGCCCTG AACGTCGTGT TATGGCCACC
    ACCATCTCAA AACTCAAACT GGACCTGGAC TTTGATAGCA CATCGATCAA CCAGATTCAA
    CTCGTTCTGT TCGGCTTTCA GGACTCTGTG AATAAAGTGT TACGGAACCA CCACATTAAA
    CCACACTGGA TGTTTGCCAT GGATAACATC ATCAGACGGG CTGTGCAGGC AGCTGTTACC
    ATTTTAATTC CAGAGCTGCA GACACATTTT GGTCCCGCAT CAGAGAGCGA GGGCGCAGAT
    AAAGACGCAG ATGAAGTAGA TGAAGAGGAC GATGCAGACT TTGGATCAGT AGCACGTACA
    ACACAGGAAG ATCAAGTTCT CACATCTGGA GTCAGCACAC TTAGTTCAGT CATATCCCAT
    GATGCGCAGC GGCTACAGCA CCCCCTAGGA GCACAGCTGG GACGACTCAA ACAGGAAACT
    TGCAGGCTTC TGGAGGAGCT GGTGCAGAAG GAGAGAGAAT ATCAGCAGGT TCTGAGACAA
    ACTCTCCAAC AGCGGGTACA TGACATCGAG CTTATCAGGA TCAGACAGCA GCCTGCTGCA
    GAGATGCCCA CTACACCCTC AGTATTTCAC GTTACTGCAC AACCCGAGCC TGACAAGAAG
    CTGACGGATT GGCTGAAGGA GCAGGGAGCA GACAGCGAGA CGATAGACAA GTTTGTTGCA
    GAAGATTATT CACTCAGCGA CGTTCTCTGC GATATTACTA AAGAAGATCT TCGATGCCTG
    AGGCTTAGGG GTGGAATGCT GTGTCGGATC TGGAAAGCCA TTCAACAACA TCGCAACAAA
    GGACTTCGAA AGGTCAAAAA CGACGAGGGA AATTCAGAAG GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.