LOC100333880 gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_021478360.1	XP_021334035.1	citron Rho-interacting kinase-like isoform X5
XM_009303721.3	XP_009301996.2	citron Rho-interacting kinase-like isoform X7
XM_017357404.2	XP_017212893.1	citron Rho-interacting kinase-like isoform X8
XM_021478361.1	XP_021334036.1	citron Rho-interacting kinase-like isoform X9
XM_017357401.1	XP_017212890.1	citron Rho-interacting kinase-like isoform X1
XM_009303724.2	XP_009301999.2	citron Rho-interacting kinase-like isoform X8
XM_009303725.2	XP_009302000.2	citron Rho-interacting kinase-like isoform X9
XM_017357404.1	XP_017212893.1	citron Rho-interacting kinase-like isoform X6
XM_017357405.1	XP_017212894.1	citron Rho-interacting kinase-like isoform X10
XM_017357400.1	XP_017212889.1	citron Rho-interacting kinase-like isoform X1
XM_021478362.1	XP_021334037.1	citron Rho-interacting kinase-like isoform X10
XM_021478363.1	XP_021334038.1	citron Rho-interacting kinase-like isoform X11
XM_009303725.3	XP_009302000.2	citron Rho-interacting kinase-like isoform X12
XM_017357405.2	XP_017212894.1	citron Rho-interacting kinase-like isoform X13
XM_017357402.1	XP_017212891.1	citron Rho-interacting kinase-like isoform X2
XM_009303721.2	XP_009301996.2	citron Rho-interacting kinase-like isoform X4
XM_009303723.2	XP_009301998.2	citron Rho-interacting kinase-like isoform X7
XM_021478359.1	XP_021334034.1	citron Rho-interacting kinase-like isoform X4
XM_009303720.3	XP_009301995.2	citron Rho-interacting kinase-like isoform X6
XM_009303722.2	XP_009301997.2	citron Rho-interacting kinase-like isoform X5
XM_009303720.2	XP_009301995.2	citron Rho-interacting kinase-like isoform X3
XM_021478357.1	XP_021334032.1	citron Rho-interacting kinase-like isoform X1
XM_021478358.1	XP_021334033.1	citron Rho-interacting kinase-like isoform X2
XM_017357400.2	XP_017212889.1	citron Rho-interacting kinase-like isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa143754	XM_021478363.1 Latest version!	Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa143749	XM_021478357.1 Latest version!	Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODa143750	XM_021478358.1 Latest version!	Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODa143751	XM_021478359.1 Latest version!	Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODa143752	XM_021478360.1 Latest version!	Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODa143753	XM_021478361.1 Latest version!	Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODa143753	XM_021478362.1 Latest version!	Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODa60005	XM_017357401.1 Latest version!	Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODa60005	XM_017357400.2 Latest version!	Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODa60006	XM_017357402.1 Latest version!	Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODa60007	XM_009303720.3 Latest version!	Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODa60008	XM_009303721.3 Latest version!	Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODa60009	XM_009303722.2 Latest version!	Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODa60010	XM_017357404.2 Latest version!	Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODa60011	XM_009303724.2 Latest version!	Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODa60011	XM_009303723.2 Latest version!	Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODa60012	XM_009303725.3 Latest version!	Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODa60013	XM_017357405.2 Latest version!	Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
ODa60010	XM_017357404.1	Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODa60012	XM_009303725.2	Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODa60013	XM_017357405.1	Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODa60008	XM_009303721.2	Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODa60007	XM_009303720.2	Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODa60005	XM_017357400.1	Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
Hide Old Accession Versions >>

LOC100333880 ( XM_021478363.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334038.1 Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X11, mRNA.

Price & Availability↑

CloneID	ODa143754
Clone ID Related Accession (Same CDS sequence)	XM_021478363.1
Accession Version	XM_021478363.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2610bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	citron Rho-interacting kinase-like isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAGTAC CCACCTACAT CATCAAAAAG GATGCATGTA AAAGGCAGGT GAAAACTGAC 
GAGGGTCTGC TTGTGATGGG ATTGACCACA TCTCAGTGTA AGAGTAAAGT GAGCGTGAGC 
CTGGGCGATT CACTGGCTCT GGTGTTGTGG GTGCAGGGGC TGTTTGGTGG CCGGCGGCGG 
GAGGATCTGC TAGCGGCGCT GGCGGCTCAG GGACAGACAC AGGTGCCTGT GGTGCCCGCC 
GCCACTCCTG TGCCCCTGCA GTACAGCGAC CTGAAGGCGG CGCTGGATAA AGAGCGAGCC 
CGATGCTCCG AGCTGGAGGA CGCCCTGCAG AAAACACGCC TGGAGCTCAG AGAAGTTCAG 
TTTAATAAAG CTCTGGAGCA CAGCTCTTCT CCAGCCACTC CAGGTTTGGC CCGTCAGCAG 
CTCATGATGT CCACCAAGTC CAACAAGTCT CCAGAGCACC AGTCCAGCGG CGGCCTGATG 
TCCTCAAACT CCAGCCGCGG ACGCAAAGAC AACGCCAATC CTGAAGAAAT AAGGAGAGTC 
ACTTCTGAAA AGTACACCAG ACGTGTGAAG GACCGGATTC ATCACAACAC TCCTCACCGC 
TTCACGCAGG GGCTCAATAT GAGAGCGGCT CGCTGTACTG TGTGTCTGGA CACAGTCCAT 
TTTGGACGTC AGGCTGCAAC ATGCATTGAA TGTCACGCGT TGTGTCACCC AAAATGCTCC 
CCGTACCTCC CTGCCACCTG CGGCGTTCCC ACTGAATACG CGCTGCACCT CTCAGAGGCT 
CTGTGTCGGG AGAAGGGAAG CACTTCCGGC CTGAAAGATA TGGGTGGACA TATGAGGCTT 
GAAGGATGGC TGAAGCAGCC CAGGAACGGG AAGCGCGGGC AGGGCTGGGA GAGGAAGTAT 
GTGGTGCTGG ATGGATATAA AGTGATTACA TTCGAGACAG AACCAAGAGA CGAGTCAGTG 
AAGCCGCTGG ATGAGTTTGA GCTGTGTCTC TCTGATGGAG AAGTGACCGT TCATTCTGCT 
GTGGGTTCCT CTGAGCTCCC CAACACCGCT AAAACAGATG TGCCGTACGT GCTGAAGCTG 
GAGTCGTCTC CTCAGTCCAC ATGCTGGCCC GGTCAGACTC TGTACCTCAT GACCTCGGGA 
TACTCAGAGA AGCAGCGCTG GGTGGCAGTG CTGGAAGCCA TCGTGGCCAG CAGTCGCGGA 
GCCAGAGAGA AAGCTGAGGC AGATGCTAAA TTATTGGGTA ACTCTTTACT TAAGCTAGAA 
GGTGACGACC GTCTGGATAT CAACTGCACC CTGCCGCTGA CCGACCAGAT TGTGTTGGTG 
GGCTCTGAGG AGGGTCTCTA CGCACTGAAT GTGATTAAGA ACTCCCTCAC TCATATCCCG 
GGTCTGGGAT CCGTGTTTCA GATCCACATT ATCAAAGATC ATGACAAGCT GCTGATGATC 
GTGGGAGACG AGCGAGCGCT GTGTCTGGTG GAGATTAAGA AGGTGAAGCA GTCTTTATCC 
CAGTCTCACC TCCCCACACT GCCCGAGATT GTGCCGTATA TCTTTGAGAC CGTCAAGGGC 
TGTCATCTGT TTGCCTCGGG CAGGATAGAA AATGGCCCAT GCATTTGTGC TGCGATGCCC 
AATAAAGTCA CGATTCTACG ATACAATGAC AATCTTAACA AGTTCTGCAT ACGGAAGGAG 
ATTGAAACTC TGGAGCCGTG CAGCTGCATC CACTTCACCA GCTACAGCAT CATCATTGGT 
ACCAATAAAT TCTACGAGAT CGAGATGAAG CAGTGCGTTC TGGACGAGTT CCTGGACAAG 
AATGACGTGT CGCTGGCGTC GGCTGTCTTC ACCGGATCCT CCCACAGTTT CCCCATCTCT 
ATCGTCCAGG TGTCCAGCAC TCCGCAGAAG GAGGAATATC TGCTGTGTTT CCACGAGATC 
GGAGTTTTTG TGGATGCTTA CGGTCGAAGA AGTCGCCCTG ATGATCTGAA GTGGAGTCGA 
CTTCCCCTGA CGTTCGCCTT CAGAGAGCCG TATCTCTTTG TGACCTACTT CAACTCTCTG 
GATGTTATTG AGATTCAGGG ACACGCAGCT CTTGGGCCTC CCAGTCTGGT TCACCTGGAC 
ATTCCTAACC CACGTTATCT GGGTCCAGCC ATATCGTCTG GTGCCATCTA TCTGGCCTCG 
TCCTACCAGA ACAAACTGCG GGTGATCTGC TGTAAGGGCA GTCTGGTGCG GGAGTCTGGA 
GAGCTGCAGA GGACCGGCTC CAGCAGGAGC AGCCCCAACA AGCGCGGTCC ACCAACATAC 
AACGAGCACA TCTCCAAACG TCTGGCCTCG AGTCCTGGAG CCCAGGAGGG GCCCCTGCAC 
CGCGAGCCCA GCACTCCTCA CCGCTACCGC GAGGGCCGCA CCGAGTTCCG GAGAGACAAA 
TCTCCAGCCC GGCCCCTGGA GCGAGAGAAA TCCCCGGGCC GTCCGGTGCT AGACAGCCGC 
AGAGAGAGGT CTCCCGGTCG CTTCGAGAGC AGCGGCAGCG AGAGAGACCG CGAGCGGACA 
CGACTGCACA CTAGCTCTGT ACGCACCCAG CTGACCCCCG TCAACAAGGT ACGCATGAGA 
CACTGCCACG TAAACTGTGT GTCCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334038.1
CDS	1122..3731
Translation	MLVPTYIIKKDACKRQVKTDEGLLVMGLTTSQCKSKVSVSLGDSLALVLWVQGLFGGRRREDLLAALAAQGQTQVPVVPAATPVPLQYSDLKAALDKERARCSELEDALQKTRLELREVQFNKALEHSSSPATPGLARQQLMMSTKSNKSPEHQSSGGLMSSNSSRGRKDNANPEEIRRVTSEKYTRRVKDRIHHNTPHRFTQGLNMRAARCTVCLDTVHFGRQAATCIECHALCHPKCSPYLPATCGVPTEYALHLSEALCREKGSTSGLKDMGGHMRLEGWLKQPRNGKRGQGWERKYVVLDGYKVITFETEPRDESVKPLDEFELCLSDGEVTVHSAVGSSELPNTAKTDVPYVLKLESSPQSTCWPGQTLYLMTSGYSEKQRWVAVLEAIVASSRGAREKAEADAKLLGNSLLKLEGDDRLDINCTLPLTDQIVLVGSEEGLYALNVIKNSLTHIPGLGSVFQIHIIKDHDKLLMIVGDERALCLVEIKKVKQSLSQSHLPTLPEIVPYIFETVKGCHLFASGRIENGPCICAAMPNKVTILRYNDNLNKFCIRKEIETLEPCSCIHFTSYSIIIGTNKFYEIEMKQCVLDEFLDKNDVSLASAVFTGSSHSFPISIVQVSSTPQKEEYLLCFHEIGVFVDAYGRRSRPDDLKWSRLPLTFAFREPYLFVTYFNSLDVIEIQGHAALGPPSLVHLDIPNPRYLGPAISSGAIYLASSYQNKLRVICCKGSLVRESGELQRTGSSRSSPNKRGPPTYNEHISKRLASSPGAQEGPLHREPSTPHRYREGRTEFRRDKSPARPLEREKSPGRPVLDSRRERSPGRFESSGSERDRERTRLHTSSVRTQLTPVNKVRMRHCHVNCVSF

Target ORF information:

RefSeq Version	XM_021478363.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478363.1

ORF Insert Sequence:

ATGCTAGTAC CCACCTACAT CATCAAAAAG GATGCATGTA AAAGGCAGGT GAAAACTGAC 
GAGGGTCTGC TTGTGATGGG ATTGACCACA TCTCAGTGTA AGAGTAAAGT GAGCGTGAGC 
CTGGGCGATT CACTGGCTCT GGTGTTGTGG GTGCAGGGGC TGTTTGGTGG CCGGCGGCGG 
GAGGATCTGC TAGCGGCGCT GGCGGCTCAG GGACAGACAC AGGTGCCTGT GGTGCCCGCC 
GCCACTCCTG TGCCCCTGCA GTACAGCGAC CTGAAGGCGG CGCTGGATAA AGAGCGAGCC 
CGATGCTCCG AGCTGGAGGA CGCCCTGCAG AAAACACGCC TGGAGCTCAG AGAAGTTCAG 
TTTAATAAAG CTCTGGAGCA CAGCTCTTCT CCAGCCACTC CAGGTTTGGC CCGTCAGCAG 
CTCATGATGT CCACCAAGTC CAACAAGTCT CCAGAGCACC AGTCCAGCGG CGGCCTGATG 
TCCTCAAACT CCAGCCGCGG ACGCAAAGAC AACGCCAATC CTGAAGAAAT AAGGAGAGTC 
ACTTCTGAAA AGTACACCAG ACGTGTGAAG GACCGGATTC ATCACAACAC TCCTCACCGC 
TTCACGCAGG GGCTCAATAT GAGAGCGGCT CGCTGTACTG TGTGTCTGGA CACAGTCCAT 
TTTGGACGTC AGGCTGCAAC ATGCATTGAA TGTCACGCGT TGTGTCACCC AAAATGCTCC 
CCGTACCTCC CTGCCACCTG CGGCGTTCCC ACTGAATACG CGCTGCACCT CTCAGAGGCT 
CTGTGTCGGG AGAAGGGAAG CACTTCCGGC CTGAAAGATA TGGGTGGACA TATGAGGCTT 
GAAGGATGGC TGAAGCAGCC CAGGAACGGG AAGCGCGGGC AGGGCTGGGA GAGGAAGTAT 
GTGGTGCTGG ATGGATATAA AGTGATTACA TTCGAGACAG AACCAAGAGA CGAGTCAGTG 
AAGCCGCTGG ATGAGTTTGA GCTGTGTCTC TCTGATGGAG AAGTGACCGT TCATTCTGCT 
GTGGGTTCCT CTGAGCTCCC CAACACCGCT AAAACAGATG TGCCGTACGT GCTGAAGCTG 
GAGTCGTCTC CTCAGTCCAC ATGCTGGCCC GGTCAGACTC TGTACCTCAT GACCTCGGGA 
TACTCAGAGA AGCAGCGCTG GGTGGCAGTG CTGGAAGCCA TCGTGGCCAG CAGTCGCGGA 
GCCAGAGAGA AAGCTGAGGC AGATGCTAAA TTATTGGGTA ACTCTTTACT TAAGCTAGAA 
GGTGACGACC GTCTGGATAT CAACTGCACC CTGCCGCTGA CCGACCAGAT TGTGTTGGTG 
GGCTCTGAGG AGGGTCTCTA CGCACTGAAT GTGATTAAGA ACTCCCTCAC TCATATCCCG 
GGTCTGGGAT CCGTGTTTCA GATCCACATT ATCAAAGATC ATGACAAGCT GCTGATGATC 
GTGGGAGACG AGCGAGCGCT GTGTCTGGTG GAGATTAAGA AGGTGAAGCA GTCTTTATCC 
CAGTCTCACC TCCCCACACT GCCCGAGATT GTGCCGTATA TCTTTGAGAC CGTCAAGGGC 
TGTCATCTGT TTGCCTCGGG CAGGATAGAA AATGGCCCAT GCATTTGTGC TGCGATGCCC 
AATAAAGTCA CGATTCTACG ATACAATGAC AATCTTAACA AGTTCTGCAT ACGGAAGGAG 
ATTGAAACTC TGGAGCCGTG CAGCTGCATC CACTTCACCA GCTACAGCAT CATCATTGGT 
ACCAATAAAT TCTACGAGAT CGAGATGAAG CAGTGCGTTC TGGACGAGTT CCTGGACAAG 
AATGACGTGT CGCTGGCGTC GGCTGTCTTC ACCGGATCCT CCCACAGTTT CCCCATCTCT 
ATCGTCCAGG TGTCCAGCAC TCCGCAGAAG GAGGAATATC TGCTGTGTTT CCACGAGATC 
GGAGTTTTTG TGGATGCTTA CGGTCGAAGA AGTCGCCCTG ATGATCTGAA GTGGAGTCGA 
CTTCCCCTGA CGTTCGCCTT CAGAGAGCCG TATCTCTTTG TGACCTACTT CAACTCTCTG 
GATGTTATTG AGATTCAGGG ACACGCAGCT CTTGGGCCTC CCAGTCTGGT TCACCTGGAC 
ATTCCTAACC CACGTTATCT GGGTCCAGCC ATATCGTCTG GTGCCATCTA TCTGGCCTCG 
TCCTACCAGA ACAAACTGCG GGTGATCTGC TGTAAGGGCA GTCTGGTGCG GGAGTCTGGA 
GAGCTGCAGA GGACCGGCTC CAGCAGGAGC AGCCCCAACA AGCGCGGTCC ACCAACATAC 
AACGAGCACA TCTCCAAACG TCTGGCCTCG AGTCCTGGAG CCCAGGAGGG GCCCCTGCAC 
CGCGAGCCCA GCACTCCTCA CCGCTACCGC GAGGGCCGCA CCGAGTTCCG GAGAGACAAA 
TCTCCAGCCC GGCCCCTGGA GCGAGAGAAA TCCCCGGGCC GTCCGGTGCT AGACAGCCGC 
AGAGAGAGGT CTCCCGGTCG CTTCGAGAGC AGCGGCAGCG AGAGAGACCG CGAGCGGACA 
CGACTGCACA CTAGCTCTGT ACGCACCCAG CTGACCCCCG TCAACAAGGT ACGCATGAGA 
CACTGCCACG TAAACTGTGT GTCCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100333880 ( XM_021478357.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334032.1 Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa143749
Clone ID Related Accession (Same CDS sequence)	XM_021478357.1
Accession Version	XM_021478357.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	citron Rho-interacting kinase-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG AGATTTCCAA AGTGCAGCGT AAGATGTCAG ACTTGGAGTC AGTTTTGCAG 
CAGAAAGACG TGGAGCTCAA GGCTTCAGAA ACCCAGAGAA ACATCTTAGA GCAAGACCTG 
GCAACCTATA TCACCGAATG CAGCAGTTTG AAGCGCAGTT TGGAGCAGGC CCGCATGGAG 
GTTTCGCAGG AAGATGATAA AGCTCTTCAG CTGCTGCACG ACATTCGAGA GCAGAGCAAC 
AAACTGCAGG AAATCAAAGA ACAGGAGTAC CACGCTCAGC TGGAGGAGAT GCGAGTGTCT 
ATTCGTCAGC TGGAAGAGAA TCTGTCGGCC GCTCGTCGCC GCAGTGACCT GTACGAGTCT 
GAGCTCAAAG AGTCCCGTCA GACCAGTGAG GAGCTCAAGA GGAAGGCAGT GGAGTATCAG 
CAGAAGATCC AGAAGGCTAA AGAACAAGGG AAAGTTGAGA TGGAGGAGCT TCTGAGCAAA 
CTGCAGAAGA CCAATGGAGA GCAACAAGTA AAGATTGAAG AGCTCCAGGA GAAGCTTGCT 
AAGGCAGTGA AGGCGAGTAC TGAAGCCACT GAACTCCTCC AGAATGTCCG TCAGGCTAAA 
GAGCGAATGG AGCGAGACCT GGAGCGTCTG CAGACCAAAG AGGACTCGTC CGACAGCCTG 
CGCAGACGCT TACGAGAGAC TGAGGATGGC AAAAAATCCC TGGAGAACCA GGTGAAGAGG 
CTGGAGATGG TGGAGCGGAG AGAATCCAAA CTGAAAGACG ACATTCAGAC CAAATCGCAG 
CAGATCCAGC AGATGGCCGA CAAAATCATG GAGCTTGAAG AAAATTTGCG GGACTCGCAG 
GCGATGGCGC AGCGCATGGA AACACAGCTG GAGCAGAAGG AAAAGCTTTT TGAAGACAAG 
ATCAAGGTTT TAGAAACTCA AATGAAGGCT GATTTGGCTG AGAAGGATTT GCTGGAGTCC 
AAACAGAGCA CTTTTGAAGA GGAGTCTCGC GAACGGGGAA AAGTGATCAG CGACCAGGCA 
GCGACGATCA ATGCCATGGA GTCAAAGATG ACCAGTCTGG AGCAGAGGAT CGCTGAGCTG 
TCCGAAGCCA ACAAACTGGC AGCAAACAGC AGCATTTACA CACAGAAGAA CATGAAAGCC 
CAGGAGGAGA TGATTTCTGA ACTCAGACAG CAGAAGTTTT ATTTGGAGTC TCAGGCTGGA 
AAACTGGAGG CGCAGAATGC AAAGCTGGAG GAGCATCTGG AGAAACTCAC CCAACAAGAG 
CAGACAAACA AGAGCCGAGT GCTGGAGCTG GAAGCCAGGC TGCGAGAGAT CGGCCTAGAG 
CATGAGGAAC AGAAGCTGGA GATCAAGCGT CAGGTGTCGG AGCTCACGTT GTCTCTGCAG 
GAGAGAGAAG CTCAGATCAG CAGCCTGCAA GCGGCACGCC ACGCTCTCGA CAGCCAACTA 
CAGCAGGCCA AAACTGAGCT GGAGGAGACC ACCGCCGAGG CCGAAGAGGA GATCACCGTC 
CTGAGGGCCC ATCGGGATGA AATCCAACGC AAGTTTGATG CCCTGCGAGA CAGCTGTGCG 
GTGATCACGG ATCTGGAGGA GCAGTTGACG CAGCTCACGC AAGAAAACGC GGAGCTGAAC 
CGGCAGAACT TCTACCTGTC CAAACAGCTG GACGAGGCCA CAGACGAGAA CGAGGAGCGC 
CTGCAGCTCA AGCAGGACGT GGAGCGCCTC AGGAGAGAGG TGGCAGACCG AGAGATGCAC 
CTCAACAACC AGAAACAGGT GACACGGCAA CACACACACA CACACACACA CACACACACA 
CACAGGACAG AGGTGGCAGA CCGAGAGATG CACCTCAACA ACCAGAAACA GAACATCGAG 
ACGCTGAAGA CGACATGTAC AATGCTGGAG GAGCAGGTTA TGGAGCTGGA GACTCTGAAT 
GATGAGCTGA TGGAGAAGGA GAGGCAGTGG GAGAACTGGA GATCTGCTCT GGACGAGGAG 
AAAAACCAGG CCGAGCGCAG AGCCAGAGAC CTGCAAAGAC AGCTGGACAG CGAGAAGCAG 
AACCGACTGC GTGCAGAACA GAGAAACTCT GAGTCTCGGC AGGCCGTGGA GCTGGCGGTG 
AAAGAGCACA AAGCTGAAAT CCTGGTTCTG CAGCAGGCGC TCAAAGACCA GAAACTCAAG 
GCTGAGGGCC TTTCTGACAC TCTGGGTGAT CTGGAGAAGA AGCACGGCAT GCTGGAGATG 
AACGCTCGCA GCCTGCAGCA GAAGCTGGAG ACGGAGAGAG AGATGAAGCA GAGACTCATG 
GACGAGCAAG CTAAACTGCA GCAGCAGCTG GACATGCAGA AGAGCCACAT CTTCAGACTG 
ACTCAAGGAC TGCAGGATGC GCTGGACCAG ACCGACCTGC TGAAGACCGA GCGCACAGAC 
CTGGAGTACC AGCTGGAAAA CATACAGGCG GCGTATTCTC ACGAGAAGGT GAAGATGGAG 
GGGACGATCA CCCAGCAGAC CAAGCTCATA GACTTCCTGC AGGCCAAGGT AGAGCACCCC 
TCTAAGAAGA AAAAGGGGCT GTTTGGTGGC CGGCGGCGGG AGGATCTGCT AGCGGCGCTG 
GCGGCTCAGG GACAGACACA GGTGCCTGTG GTGCCCGCCG CCACTCCTGT GCCCCTGCAG 
TACAGCGACC TGAAGGCGGC GCTGGATAAA GAGCGAGCCC GATGCTCCGA GCTGGAGGAC 
GCCCTGCAGA AAACACGCCT GGAGCTCAGA GAAGTTCAGT TTAATAAAGC TCTGGAGCAC 
AGCTCTTCTC CAGCCACTCC AGGTTTGGCC CGTCAGCAGC TCATGATGTC CACCAAGTCC 
AACAAGTCTC CAGAGCACCA GTCCAGCGGC GGCCTGATGT CCTCAAACTC CAGCCGCGGA 
CGCAAAGACA ACGCCAATCC TGAAGAAATA AGGAGAGTCA CTTCTGAAAA GTACACCAGA 
CGTGTGAAGG ACCGGATTCA TCACAACACT CCTCACCGCT TCACGCAGGG GCTCAATATG 
AGAGCGGCTC GCTGTACTGT GTGTCTGGAC ACAGTCCATT TTGGACGTCA GGCTGCAACA 
TGCATTGAAT GTCACGCGTT GTGTCACCCA AAATGCTCCC CGTACCTCCC TGCCACCTGC 
GGCGTTCCCA CTGAATACGC GCTGCACCTC TCAGAGGCTC TGTGTCGGGA GAAGGGAAGC 
ACTTCCGGCC TGAAAGATAT GGGTGGACAT ATGAGGCTTG AAGGATGGCT GAAGCAGCCC 
AGGAACGGGA AGCGCGGGCA GGGCTGGGAG AGGAAGTATG TGGTGCTGGA TGGATATAAA 
GTGATTACAT TCGAGACAGA ACCAAGAGAC GAGTCAGTGA AGCCGCTGGA TGAGTTTGAG 
CTGTGTCTCT CTGATGGAGA AGTGACCGTT CATTCTGCTG TGGGTTCCTC TGAGCTCCCC 
AACACCGCTA AAACAGATGT GCCGTACGTG CTGAAGCTGG AGTCGTCTCC TCAGTCCACA 
TGCTGGCCCG GTCAGACTCT GTACCTCATG ACCTCGGGAT ACTCAGAGAA GCAGCGCTGG 
GTGGCAGTGC TGGAAGCCAT CGTGGCCAGC AGTCGCGGAG CCAGAGAGAA AGCTGAGGCA 
GATGCTAAAT TATTGGGTAA CTCTTTACTT AAGCTAGAAG GTGACGACCG TCTGGATATC 
AACTGCACCC TGCCGCTGAC CGACCAGATT GTGTTGGTGG GCTCTGAGGA GGGTCTCTAC 
GCACTGAATG TGATTAAGAA CTCCCTCACT CATATCCCGG GTCTGGGATC CGTGTTTCAG 
ATCCACATTA TCAAAGATCA TGACAAGCTG CTGATGATCG TGGGAGACGA GCGAGCGCTG 
TGTCTGGTGG AGATTAAGAA GGTGAAGCAG TCTTTATCCC AGTCTCACCT CCCCACACTG 
CCCGAGATTG TGCCGTATAT CTTTGAGACC GTCAAGGGCT GTCATCTGTT TGCCTCGGGC 
AGGATAGAAA ATGGCCCATG CATTTGTGCT GCGATGCCCA ATAAAGTCAC GATTCTACGA 
TACAATGACA ATCTTAACAA GTTCTGCATA CGGAAGGAGA TTGAAACTCT GGAGCCGTGC 
AGCTGCATCC ACTTCACCAG CTACAGCATC ATCATTGGTA CCAATAAATT CTACGAGATC 
GAGATGAAGC AGTGCGTTCT GGACGAGTTC CTGGACAAGA ATGACGTGTC GCTGGCGTCG 
GCTGTCTTCA CCGGATCCTC CCACAGTTTC CCCATCTCTA TCGTCCAGGT GTCCAGCACT 
CCGCAGAAGG AGGAATATCT GCTGTGTTTC CACGAGATCG GAGTTTTTGT GGATGCTTAC 
GGTCGAAGAA GTCGCCCTGA TGATCTGAAG TGGAGTCGAC TTCCCCTGAC GTTCGCCTTC 
AGAGAGCCGT ATCTCTTTGT GACCTACTTC AACTCTCTGG ATGTTATTGA GATTCAGGGA 
CACGCAGCTC TTGGGCCTCC CAGTCTGGTT CACCTGGACA TTCCTAACCC ACGTTATCTG 
GGTCCAGCCA TATCGTCTGG TGCCATCTAT CTGGCCTCGT CCTACCAGAA CAAACTGCGG 
GTGATCTGCT GTAAGGGCAG TCTGGTGCGG GAGTCTGGAG AGCTGCAGAG GACCGGCTCC 
AGCAGGAGCA GCCCCAACAA GCGCGGTCCA CCAACATACA ACGAGCACAT CTCCAAACGT 
CTGGCCTCGA GTCCTGGAGC CCAGGAGGGG CCCCTGCACC GCGAGCCCAG CACTCCTCAC 
CGCTACCGCG AGGGCCGCAC CGAGTTCCGG AGAGACAAAT CTCCAGCCCG GCCCCTGGAG 
CGAGAGAAAT CCCCGGGCCG TCCGGTGCTA GACAGCCGCA GAGAGAGGTC TCCCGGTCGC 
TTCGAGAGCA GCGGCAGCGA GAGAGACCGC GAGCGGACAC GACTGCACAC TAGCTCTGTA 
CGCACCCAGC TGACCCCCGT CAACAAGGTA CGCATGAGAC ACTGCCACGT AAACTGTGTG 
TCCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334032.1
CDS	433..5421
Translation	MEQEISKVQRKMSDLESVLQQKDVELKASETQRNILEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSNKLQEIKEQEYHAQLEEMRVSIRQLEENLSAARRRSDLYESELKESRQTSEELKRKAVEYQQKIQKAKEQGKVEMEELLSKLQKTNGEQQVKIEELQEKLAKAVKASTEATELLQNVRQAKERMERDLERLQTKEDSSDSLRRRLRETEDGKKSLENQVKRLEMVERRESKLKDDIQTKSQQIQQMADKIMELEENLRDSQAMAQRMETQLEQKEKLFEDKIKVLETQMKADLAEKDLLESKQSTFEEESRERGKVISDQAATINAMESKMTSLEQRIAELSEANKLAANSSIYTQKNMKAQEEMISELRQQKFYLESQAGKLEAQNAKLEEHLEKLTQQEQTNKSRVLELEARLREIGLEHEEQKLEIKRQVSELTLSLQEREAQISSLQAARHALDSQLQQAKTELEETTAEAEEEITVLRAHRDEIQRKFDALRDSCAVITDLEEQLTQLTQENAELNRQNFYLSKQLDEATDENEERLQLKQDVERLRREVADREMHLNNQKQVTRQHTHTHTHTHTHRTEVADREMHLNNQKQNIETLKTTCTMLEEQVMELETLNDELMEKERQWENWRSALDEEKNQAERRARDLQRQLDSEKQNRLRAEQRNSESRQAVELAVKEHKAEILVLQQALKDQKLKAEGLSDTLGDLEKKHGMLEMNARSLQQKLETEREMKQRLMDEQAKLQQQLDMQKSHIFRLTQGLQDALDQTDLLKTERTDLEYQLENIQAAYSHEKVKMEGTITQQTKLIDFLQAKVEHPSKKKKGLFGGRRREDLLAALAAQGQTQVPVVPAATPVPLQYSDLKAALDKERARCSELEDALQKTRLELREVQFNKALEHSSSPATPGLARQQLMMSTKSNKSPEHQSSGGLMSSNSSRGRKDNANPEEIRRVTSEKYTRRVKDRIHHNTPHRFTQGLNMRAARCTVCLDTVHFGRQAATCIECHALCHPKCSPYLPATCGVPTEYALHLSEALCREKGSTSGLKDMGGHMRLEGWLKQPRNGKRGQGWERKYVVLDGYKVITFETEPRDESVKPLDEFELCLSDGEVTVHSAVGSSELPNTAKTDVPYVLKLESSPQSTCWPGQTLYLMTSGYSEKQRWVAVLEAIVASSRGAREKAEADAKLLGNSLLKLEGDDRLDINCTLPLTDQIVLVGSEEGLYALNVIKNSLTHIPGLGSVFQIHIIKDHDKLLMIVGDERALCLVEIKKVKQSLSQSHLPTLPEIVPYIFETVKGCHLFASGRIENGPCICAAMPNKVTILRYNDNLNKFCIRKEIETLEPCSCIHFTSYSIIIGTNKFYEIEMKQCVLDEFLDKNDVSLASAVFTGSSHSFPISIVQVSSTPQKEEYLLCFHEIGVFVDAYGRRSRPDDLKWSRLPLTFAFREPYLFVTYFNSLDVIEIQGHAALGPPSLVHLDIPNPRYLGPAISSGAIYLASSYQNKLRVICCKGSLVRESGELQRTGSSRSSPNKRGPPTYNEHISKRLASSPGAQEGPLHREPSTPHRYREGRTEFRRDKSPARPLEREKSPGRPVLDSRRERSPGRFESSGSERDRERTRLHTSSVRTQLTPVNKVRMRHCHVNCVSF

Target ORF information:

RefSeq Version	XM_021478357.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478357.1

ORF Insert Sequence:

ATGGAGCAGG AGATTTCCAA AGTGCAGCGT AAGATGTCAG ACTTGGAGTC AGTTTTGCAG 
CAGAAAGACG TGGAGCTCAA GGCTTCAGAA ACCCAGAGAA ACATCTTAGA GCAAGACCTG 
GCAACCTATA TCACCGAATG CAGCAGTTTG AAGCGCAGTT TGGAGCAGGC CCGCATGGAG 
GTTTCGCAGG AAGATGATAA AGCTCTTCAG CTGCTGCACG ACATTCGAGA GCAGAGCAAC 
AAACTGCAGG AAATCAAAGA ACAGGAGTAC CACGCTCAGC TGGAGGAGAT GCGAGTGTCT 
ATTCGTCAGC TGGAAGAGAA TCTGTCGGCC GCTCGTCGCC GCAGTGACCT GTACGAGTCT 
GAGCTCAAAG AGTCCCGTCA GACCAGTGAG GAGCTCAAGA GGAAGGCAGT GGAGTATCAG 
CAGAAGATCC AGAAGGCTAA AGAACAAGGG AAAGTTGAGA TGGAGGAGCT TCTGAGCAAA 
CTGCAGAAGA CCAATGGAGA GCAACAAGTA AAGATTGAAG AGCTCCAGGA GAAGCTTGCT 
AAGGCAGTGA AGGCGAGTAC TGAAGCCACT GAACTCCTCC AGAATGTCCG TCAGGCTAAA 
GAGCGAATGG AGCGAGACCT GGAGCGTCTG CAGACCAAAG AGGACTCGTC CGACAGCCTG 
CGCAGACGCT TACGAGAGAC TGAGGATGGC AAAAAATCCC TGGAGAACCA GGTGAAGAGG 
CTGGAGATGG TGGAGCGGAG AGAATCCAAA CTGAAAGACG ACATTCAGAC CAAATCGCAG 
CAGATCCAGC AGATGGCCGA CAAAATCATG GAGCTTGAAG AAAATTTGCG GGACTCGCAG 
GCGATGGCGC AGCGCATGGA AACACAGCTG GAGCAGAAGG AAAAGCTTTT TGAAGACAAG 
ATCAAGGTTT TAGAAACTCA AATGAAGGCT GATTTGGCTG AGAAGGATTT GCTGGAGTCC 
AAACAGAGCA CTTTTGAAGA GGAGTCTCGC GAACGGGGAA AAGTGATCAG CGACCAGGCA 
GCGACGATCA ATGCCATGGA GTCAAAGATG ACCAGTCTGG AGCAGAGGAT CGCTGAGCTG 
TCCGAAGCCA ACAAACTGGC AGCAAACAGC AGCATTTACA CACAGAAGAA CATGAAAGCC 
CAGGAGGAGA TGATTTCTGA ACTCAGACAG CAGAAGTTTT ATTTGGAGTC TCAGGCTGGA 
AAACTGGAGG CGCAGAATGC AAAGCTGGAG GAGCATCTGG AGAAACTCAC CCAACAAGAG 
CAGACAAACA AGAGCCGAGT GCTGGAGCTG GAAGCCAGGC TGCGAGAGAT CGGCCTAGAG 
CATGAGGAAC AGAAGCTGGA GATCAAGCGT CAGGTGTCGG AGCTCACGTT GTCTCTGCAG 
GAGAGAGAAG CTCAGATCAG CAGCCTGCAA GCGGCACGCC ACGCTCTCGA CAGCCAACTA 
CAGCAGGCCA AAACTGAGCT GGAGGAGACC ACCGCCGAGG CCGAAGAGGA GATCACCGTC 
CTGAGGGCCC ATCGGGATGA AATCCAACGC AAGTTTGATG CCCTGCGAGA CAGCTGTGCG 
GTGATCACGG ATCTGGAGGA GCAGTTGACG CAGCTCACGC AAGAAAACGC GGAGCTGAAC 
CGGCAGAACT TCTACCTGTC CAAACAGCTG GACGAGGCCA CAGACGAGAA CGAGGAGCGC 
CTGCAGCTCA AGCAGGACGT GGAGCGCCTC AGGAGAGAGG TGGCAGACCG AGAGATGCAC 
CTCAACAACC AGAAACAGGT GACACGGCAA CACACACACA CACACACACA CACACACACA 
CACAGGACAG AGGTGGCAGA CCGAGAGATG CACCTCAACA ACCAGAAACA GAACATCGAG 
ACGCTGAAGA CGACATGTAC AATGCTGGAG GAGCAGGTTA TGGAGCTGGA GACTCTGAAT 
GATGAGCTGA TGGAGAAGGA GAGGCAGTGG GAGAACTGGA GATCTGCTCT GGACGAGGAG 
AAAAACCAGG CCGAGCGCAG AGCCAGAGAC CTGCAAAGAC AGCTGGACAG CGAGAAGCAG 
AACCGACTGC GTGCAGAACA GAGAAACTCT GAGTCTCGGC AGGCCGTGGA GCTGGCGGTG 
AAAGAGCACA AAGCTGAAAT CCTGGTTCTG CAGCAGGCGC TCAAAGACCA GAAACTCAAG 
GCTGAGGGCC TTTCTGACAC TCTGGGTGAT CTGGAGAAGA AGCACGGCAT GCTGGAGATG 
AACGCTCGCA GCCTGCAGCA GAAGCTGGAG ACGGAGAGAG AGATGAAGCA GAGACTCATG 
GACGAGCAAG CTAAACTGCA GCAGCAGCTG GACATGCAGA AGAGCCACAT CTTCAGACTG 
ACTCAAGGAC TGCAGGATGC GCTGGACCAG ACCGACCTGC TGAAGACCGA GCGCACAGAC 
CTGGAGTACC AGCTGGAAAA CATACAGGCG GCGTATTCTC ACGAGAAGGT GAAGATGGAG 
GGGACGATCA CCCAGCAGAC CAAGCTCATA GACTTCCTGC AGGCCAAGGT AGAGCACCCC 
TCTAAGAAGA AAAAGGGGCT GTTTGGTGGC CGGCGGCGGG AGGATCTGCT AGCGGCGCTG 
GCGGCTCAGG GACAGACACA GGTGCCTGTG GTGCCCGCCG CCACTCCTGT GCCCCTGCAG 
TACAGCGACC TGAAGGCGGC GCTGGATAAA GAGCGAGCCC GATGCTCCGA GCTGGAGGAC 
GCCCTGCAGA AAACACGCCT GGAGCTCAGA GAAGTTCAGT TTAATAAAGC TCTGGAGCAC 
AGCTCTTCTC CAGCCACTCC AGGTTTGGCC CGTCAGCAGC TCATGATGTC CACCAAGTCC 
AACAAGTCTC CAGAGCACCA GTCCAGCGGC GGCCTGATGT CCTCAAACTC CAGCCGCGGA 
CGCAAAGACA ACGCCAATCC TGAAGAAATA AGGAGAGTCA CTTCTGAAAA GTACACCAGA 
CGTGTGAAGG ACCGGATTCA TCACAACACT CCTCACCGCT TCACGCAGGG GCTCAATATG 
AGAGCGGCTC GCTGTACTGT GTGTCTGGAC ACAGTCCATT TTGGACGTCA GGCTGCAACA 
TGCATTGAAT GTCACGCGTT GTGTCACCCA AAATGCTCCC CGTACCTCCC TGCCACCTGC 
GGCGTTCCCA CTGAATACGC GCTGCACCTC TCAGAGGCTC TGTGTCGGGA GAAGGGAAGC 
ACTTCCGGCC TGAAAGATAT GGGTGGACAT ATGAGGCTTG AAGGATGGCT GAAGCAGCCC 
AGGAACGGGA AGCGCGGGCA GGGCTGGGAG AGGAAGTATG TGGTGCTGGA TGGATATAAA 
GTGATTACAT TCGAGACAGA ACCAAGAGAC GAGTCAGTGA AGCCGCTGGA TGAGTTTGAG 
CTGTGTCTCT CTGATGGAGA AGTGACCGTT CATTCTGCTG TGGGTTCCTC TGAGCTCCCC 
AACACCGCTA AAACAGATGT GCCGTACGTG CTGAAGCTGG AGTCGTCTCC TCAGTCCACA 
TGCTGGCCCG GTCAGACTCT GTACCTCATG ACCTCGGGAT ACTCAGAGAA GCAGCGCTGG 
GTGGCAGTGC TGGAAGCCAT CGTGGCCAGC AGTCGCGGAG CCAGAGAGAA AGCTGAGGCA 
GATGCTAAAT TATTGGGTAA CTCTTTACTT AAGCTAGAAG GTGACGACCG TCTGGATATC 
AACTGCACCC TGCCGCTGAC CGACCAGATT GTGTTGGTGG GCTCTGAGGA GGGTCTCTAC 
GCACTGAATG TGATTAAGAA CTCCCTCACT CATATCCCGG GTCTGGGATC CGTGTTTCAG 
ATCCACATTA TCAAAGATCA TGACAAGCTG CTGATGATCG TGGGAGACGA GCGAGCGCTG 
TGTCTGGTGG AGATTAAGAA GGTGAAGCAG TCTTTATCCC AGTCTCACCT CCCCACACTG 
CCCGAGATTG TGCCGTATAT CTTTGAGACC GTCAAGGGCT GTCATCTGTT TGCCTCGGGC 
AGGATAGAAA ATGGCCCATG CATTTGTGCT GCGATGCCCA ATAAAGTCAC GATTCTACGA 
TACAATGACA ATCTTAACAA GTTCTGCATA CGGAAGGAGA TTGAAACTCT GGAGCCGTGC 
AGCTGCATCC ACTTCACCAG CTACAGCATC ATCATTGGTA CCAATAAATT CTACGAGATC 
GAGATGAAGC AGTGCGTTCT GGACGAGTTC CTGGACAAGA ATGACGTGTC GCTGGCGTCG 
GCTGTCTTCA CCGGATCCTC CCACAGTTTC CCCATCTCTA TCGTCCAGGT GTCCAGCACT 
CCGCAGAAGG AGGAATATCT GCTGTGTTTC CACGAGATCG GAGTTTTTGT GGATGCTTAC 
GGTCGAAGAA GTCGCCCTGA TGATCTGAAG TGGAGTCGAC TTCCCCTGAC GTTCGCCTTC 
AGAGAGCCGT ATCTCTTTGT GACCTACTTC AACTCTCTGG ATGTTATTGA GATTCAGGGA 
CACGCAGCTC TTGGGCCTCC CAGTCTGGTT CACCTGGACA TTCCTAACCC ACGTTATCTG 
GGTCCAGCCA TATCGTCTGG TGCCATCTAT CTGGCCTCGT CCTACCAGAA CAAACTGCGG 
GTGATCTGCT GTAAGGGCAG TCTGGTGCGG GAGTCTGGAG AGCTGCAGAG GACCGGCTCC 
AGCAGGAGCA GCCCCAACAA GCGCGGTCCA CCAACATACA ACGAGCACAT CTCCAAACGT 
CTGGCCTCGA GTCCTGGAGC CCAGGAGGGG CCCCTGCACC GCGAGCCCAG CACTCCTCAC 
CGCTACCGCG AGGGCCGCAC CGAGTTCCGG AGAGACAAAT CTCCAGCCCG GCCCCTGGAG 
CGAGAGAAAT CCCCGGGCCG TCCGGTGCTA GACAGCCGCA GAGAGAGGTC TCCCGGTCGC 
TTCGAGAGCA GCGGCAGCGA GAGAGACCGC GAGCGGACAC GACTGCACAC TAGCTCTGTA 
CGCACCCAGC TGACCCCCGT CAACAAGGTA CGCATGAGAC ACTGCCACGT AAACTGTGTG 
TCCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100333880 ( XM_021478358.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334033.1 Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X2, mRNA.

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CloneID	ODa143750
Clone ID Related Accession (Same CDS sequence)	XM_021478358.1
Accession Version	XM_021478358.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	citron Rho-interacting kinase-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG AGATTTCCAA AGTGCAGCGT AAGATGTCAG ACTTGGAGTC AGTTTTGCAG 
CAGAAAGACG TGGAGCTCAA GGCTTCAGAA ACCCAGAGAA ACATCTTAGA GCAAGACCTG 
GCAACCTATA TCACCGAATG CAGCAGTTTG AAGCGCAGTT TGGAGCAGGC CCGCATGGAG 
GTTTCGCAGG AAGATGATAA AGCTCTTCAG CTGCTGCACG ACATTCGAGA GCAGAGCAAC 
AAACTGCAGG AAATCAAAGA ACAGGAGTAC CACGCTCAGC TGGAGGAGAT GCGAGTGTCT 
ATTCGTCAGC TGGAAGAGAA TCTGTCGGCC GCTCGTCGCC GCAGTGACCT GTACGAGTCT 
GAGCTCAAAG AGTCCCGTCA GACCAGTGAG GAGCTCAAGA GGAAGGCAGT GGAGTATCAG 
CAGAAGATCC AGAAGGCTAA AGAACAAGGG AAAGTTGAGA TGGAGGAGCT TCTGAGCAAA 
CTGCAGAAGA CCAATGGAGA GCAACAAGTA AAGATTGAAG AGCTCCAGGA GAAGCTTGCT 
AAGGCAGTGA AGGCGAGTAC TGAAGCCACT GAACTCCTCC AGAATGTCCG TCAGGCTAAA 
GAGCGAATGG AGCGAGACCT GGAGCGTCTG CAGACCAAAG AGGACTCGTC CGACAGCCTG 
CGCAGACGCT TACGAGAGAC TGAGGATGGC AAAAAATCCC TGGAGAACCA GGTGAAGAGG 
CTGGAGATGG TGGAGCGGAG AGAATCCAAA CTGAAAGACG ACATTCAGAC CAAATCGCAG 
CAGATCCAGC AGATGGCCGA CAAAATCATG GAGCTTGAAG AAAATTTGCG GGACTCGCAG 
GCGATGGCGC AGCGCATGGA AACACAGCTG GAGCAGAAGG AAAAGCTTTT TGAAGACAAG 
ATCAAGGTTT TAGAAACTCA AATGAAGGCT GATTTGGCTG AGAAGGATTT GCTGGAGTCC 
AAACAGAGCA CTTTTGAAGA GGAGTCTCGC GAACGGGGAA AAGTGATCAG CGACCAGGCA 
GCGACGATCA ATGCCATGGA GTCAAAGATG ACCAGTCTGG AGCAGAGGAT CGCTGAGCTG 
TCCGAAGCCA ACAAACTGGC AGCAAACAGC AGCATTTACA CACAGAAGAA CATGAAAGCC 
CAGGAGGAGA TGATTTCTGA ACTCAGACAG CAGAAGTTTT ATTTGGAGTC TCAGGCTGGA 
AAACTGGAGG CGCAGAATGC AAAGCTGGAG GAGCATCTGG AGAAACTCAC CCAACAAGAG 
CAGACAAACA AGAGCCGAGT GCTGGAGCTG GAAGCCAGGC TGCGAGAGAT CGGCCTAGAG 
CATGAGGAAC AGAAGCTGGA GATCAAGCGT CAGGTGTCGG AGCTCACGTT GTCTCTGCAG 
GAGAGAGAAG CTCAGATCAG CAGCCTGCAA GCGGCACGCC ACGCTCTCGA CAGCCAACTA 
CAGCAGGCCA AAACTGAGCT GGAGGAGACC ACCGCCGAGG CCGAAGAGGA GATCACCGTC 
CTGAGGGCCC ATCGGGATGA AATCCAACGC AAGTTTGATG CCCTGCGAGA CAGCTGTGCG 
GTGATCACGG ATCTGGAGGA GCAGTTGACG CAGCTCACGC AAGAAAACGC GGAGCTGAAC 
CGGCAGAACT TCTACCTGTC CAAACAGCTG GACGAGGCCA CAGACGAGAA CGAGGAGCGC 
CTGCAGCTCA AGCAGGACGT GGAGCGCCTC AGGAGAGAGG TGGCAGACCG AGAGATGCAC 
CTCAACAACC AGAAACAGGT GACACGGCAA CACACACACA CACACACACA CACACACACA 
CACAGGACAG AGGTGGCAGA CCGAGAGATG CACCTCAACA ACCAGAAACA GAACATCGAG 
ACGCTGAAGA CGACATGTAC AATGCTGGAG GAGCAGGTTA TGGAGCTGGA GACTCTGAAT 
GATGAGCTGA TGGAGAAGGA GAGGCAGTGG GAGAACTGGA GATCTGCTCT GGACGAGGAG 
AAAAACCAGG CCGAGCGCAG AGCCAGAGAC CTGCAAAGAC AGCTGGACAG CGAGAAGCAG 
AACCGACTGC GTGCAGAACA GAGAAACTCT GAGTCTCGGC AGGCCGTGGA GCTGGCGGTG 
AAAGAGCACA AAGCTGAAAT CCTGGTTCTG CAGCAGGCGC TCAAAGACCA GAAACTCAAG 
GCTGAGGGCC TTTCTGACAC TCTGGGTGAT CTGGAGAAGA AGCACGGCAT GCTGGAGATG 
AACGCTCGCA GCCTGCAGCA GAAGCTGGAG ACGGAGAGAG AGATGAAGCA GAGACTCATG 
GACGAGCAAG CTAAACTGCA GCAGCAGCTG GACATGCAGA AGAGCCACAT CTTCAGACTG 
ACTCAAGGAC TGCAGGATGC GCTGGACCAG ACCGACCTGC TGAAGACCGA GCGCACAGAC 
CTGGAGTACC AGCTGGAAAA CATACAGGCG GCGTATTCTC ACGAGAAGGT GAAGATGGAG 
GGGACGATCA CCCAGCAGAC CAAGCTCATA GACTTCCTGC AGGCCAAGGT AGAGCACCCC 
TCTAAGAAGA AAAAGGGGCT GTTTGGTGGC CGGCGGCGGG AGGATCTGCT AGCGGCGCTG 
GCGGCTCAGG GACAGACACA GGTGCCTGTG GTGCCCGCCG CCACTCCTGT GCCCCTGCAG 
TACAGCGACC TGAAGGCGGC GCTGGATAAA GAGCGAGCCC GATGCTCCGA GCTGGAGGAC 
GCCCTGCAGA AAACACGCCT GGAGCTCAGA GAAGTTCAGT TTAATAAAGC TCTGGAGCAC 
AGCTCTTCTC CAGCCACTCC AGGTTTGGCC CGTCAGCAGC TCATGATGTC CACCAAGTCC 
AACAAGTCTC CAGAGCACCA GTCCAGCGGC GGCCTGATGT CCTCAAACTC CAGCCGCGGA 
CGCAAAGACA ACGCCAATCC TGAAGAAATA AGGAGAGTCA CTTCTGAAAA GTACACCAGA 
CGTGTGAAGG ACCGGATTCA TCACAACACT CCTCACCGCT TCACGCAGGG GCTCAATATG 
AGAGCGGCTC GCTGTACTGT GTGTCTGGAC ACAGTCCATT TTGGACGTCA GGCTGCAACA 
TGCATTGAAT GTCACGCGTT GTGTCACCCA AAATGCTCCC CGTACCTCCC TGCCACCTGC 
GGCGTTCCCA CTGAATACGC GCTGCACCTC TCAGAGGCTC TGTGTCGGGA GAAGGGAAGC 
ACTTCCGGCC TGAAAGATAT GGGTGGACAT ATGAGGCTTG AAGGATGGCT GAAGCAGCCC 
AGGAACGGGA AGCGCGGGCA GGGCTGGGAG AGGAAGTATG TGGTGCTGGA TGGATATAAA 
GTGATTACAT TCGAGACAGA ACCAAGAGAC GAGTCAGTGA AGCCGCTGGA TGAGTTTGAG 
CTGTGTCTCT CTGATGGAGA AGTGACCGTT CATTCTGCTG TGGGTTCCTC TGAGCTCCCC 
AACACCGCTA AAACAGATGT GCCGTACGTG CTGAAGCTGG AGTCGTCTCC TCAGTCCACA 
TGCTGGCCCG GTCAGACTCT GTACCTCATG ACCTCGGGAT ACTCAGAGAA GCAGCGCTGG 
GTGGCAGTGC TGGAAGCCAT CGTGGCCAGC AGTCGCGGAG CCAGAGAGAA AGCTGAGGCA 
GATGCTAAAT TATTGGGTAA CTCTTTACTT AAGCTAGAAG GTGACGACCG TCTGGATATC 
AACTGCACCC TGCCGCTGAC CGACCAGATT GTGTTGGTGG GCTCTGAGGA GGGTCTCTAC 
GCACTGAATG TGATTAAGAA CTCCCTCACT CATATCCCGG GTCTGGGATC CGTGTTTCAG 
ATCCACATTA TCAAAGATCA TGACAAGCTG CTGATGATCG TGGGAGACGA GCGAGCGCTG 
TGTCTGGTGG AGATTAAGAA GGTGAAGCAG TCTTTATCCC AGTCTCACCT CCCCACACTG 
CCCGAGATTG TGCCGTATAT CTTTGAGACC GTCAAGGGCT GTCATCTGTT TGCCTCGGGC 
AGGATAGAAA ATGGCCCATG CATTTGTGCT GCGATGCCCA ATAAAGTCAC GATTCTACGA 
TACAATGACA ATCTTAACAA GTTCTGCATA CGGAAGGAGA TTGAAACTCT GGAGCCGTGC 
AGCTGCATCC ACTTCACCAG CTACAGCATC ATCATTGGTA CCAATAAATT CTACGAGATC 
GAGATGAAGC AGTGCGTTCT GGACGAGTTC CTGGACAAGA ATGACGTGTC GCTGGCGTCG 
GCTGTCTTCA CCGGATCCTC CCACAGTTTC CCCATCTCTA TCGTCCAGGT GTCCAGCACT 
CCGCAGAAGG AGGAATATCT GCTGTGTTTC CACGAGATCG GAGTTTTTGT GGATGCTTAC 
GGTCGAAGAA GTCGCCCTGA TGATCTGAAG TGGAGTCGAC TTCCCCTGAC GTTCGCCTTC 
AGAGAGCCGT ATCTCTTTGT GACCTACTTC AACTCTCTGG ATGTTATTGA GATTCAGGGA 
CACGCAGCTC TTGGGCCTCC CAGTCTGGTT CACCTGGACA TTCCTAACCC ACGTTATCTG 
GGTCCAGCCA TATCGTCTGG TGCCATCTAT CTGGCCTCGT CCTACCAGAA CAAACTGCGG 
GTGATCTGCT GTAAGGGCAG TCTGGTGCGG GAGTCTGGAG AGCTGCAGAG GACCGGCTCC 
AGCAGGAGCC CCAACAAGCG CGGTCCACCA ACATACAACG AGCACATCTC CAAACGTCTG 
GCCTCGAGTC CTGGAGCCCA GGAGGGGCCC CTGCACCGCG AGCCCAGCAC TCCTCACCGC 
TACCGCGAGG GCCGCACCGA GTTCCGGAGA GACAAATCTC CAGCCCGGCC CCTGGAGCGA 
GAGAAATCCC CGGGCCGTCC GGTGCTAGAC AGCCGCAGAG AGAGGTCTCC CGGTCGCTTC 
GAGAGCAGCG GCAGCGAGAG AGACCGCGAG CGGACACGAC TGCACACTAG CTCTGTACGC 
ACCCAGCTGA CCCCCGTCAA CAAGGTACGC ATGAGACACT GCCACGTAAA CTGTGTGTCC 
TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334033.1
CDS	434..5419
Translation	MEQEISKVQRKMSDLESVLQQKDVELKASETQRNILEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSNKLQEIKEQEYHAQLEEMRVSIRQLEENLSAARRRSDLYESELKESRQTSEELKRKAVEYQQKIQKAKEQGKVEMEELLSKLQKTNGEQQVKIEELQEKLAKAVKASTEATELLQNVRQAKERMERDLERLQTKEDSSDSLRRRLRETEDGKKSLENQVKRLEMVERRESKLKDDIQTKSQQIQQMADKIMELEENLRDSQAMAQRMETQLEQKEKLFEDKIKVLETQMKADLAEKDLLESKQSTFEEESRERGKVISDQAATINAMESKMTSLEQRIAELSEANKLAANSSIYTQKNMKAQEEMISELRQQKFYLESQAGKLEAQNAKLEEHLEKLTQQEQTNKSRVLELEARLREIGLEHEEQKLEIKRQVSELTLSLQEREAQISSLQAARHALDSQLQQAKTELEETTAEAEEEITVLRAHRDEIQRKFDALRDSCAVITDLEEQLTQLTQENAELNRQNFYLSKQLDEATDENEERLQLKQDVERLRREVADREMHLNNQKQVTRQHTHTHTHTHTHRTEVADREMHLNNQKQNIETLKTTCTMLEEQVMELETLNDELMEKERQWENWRSALDEEKNQAERRARDLQRQLDSEKQNRLRAEQRNSESRQAVELAVKEHKAEILVLQQALKDQKLKAEGLSDTLGDLEKKHGMLEMNARSLQQKLETEREMKQRLMDEQAKLQQQLDMQKSHIFRLTQGLQDALDQTDLLKTERTDLEYQLENIQAAYSHEKVKMEGTITQQTKLIDFLQAKVEHPSKKKKGLFGGRRREDLLAALAAQGQTQVPVVPAATPVPLQYSDLKAALDKERARCSELEDALQKTRLELREVQFNKALEHSSSPATPGLARQQLMMSTKSNKSPEHQSSGGLMSSNSSRGRKDNANPEEIRRVTSEKYTRRVKDRIHHNTPHRFTQGLNMRAARCTVCLDTVHFGRQAATCIECHALCHPKCSPYLPATCGVPTEYALHLSEALCREKGSTSGLKDMGGHMRLEGWLKQPRNGKRGQGWERKYVVLDGYKVITFETEPRDESVKPLDEFELCLSDGEVTVHSAVGSSELPNTAKTDVPYVLKLESSPQSTCWPGQTLYLMTSGYSEKQRWVAVLEAIVASSRGAREKAEADAKLLGNSLLKLEGDDRLDINCTLPLTDQIVLVGSEEGLYALNVIKNSLTHIPGLGSVFQIHIIKDHDKLLMIVGDERALCLVEIKKVKQSLSQSHLPTLPEIVPYIFETVKGCHLFASGRIENGPCICAAMPNKVTILRYNDNLNKFCIRKEIETLEPCSCIHFTSYSIIIGTNKFYEIEMKQCVLDEFLDKNDVSLASAVFTGSSHSFPISIVQVSSTPQKEEYLLCFHEIGVFVDAYGRRSRPDDLKWSRLPLTFAFREPYLFVTYFNSLDVIEIQGHAALGPPSLVHLDIPNPRYLGPAISSGAIYLASSYQNKLRVICCKGSLVRESGELQRTGSSRSPNKRGPPTYNEHISKRLASSPGAQEGPLHREPSTPHRYREGRTEFRRDKSPARPLEREKSPGRPVLDSRRERSPGRFESSGSERDRERTRLHTSSVRTQLTPVNKVRMRHCHVNCVSF

Target ORF information:

RefSeq Version	XM_021478358.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478358.1

ORF Insert Sequence:

ATGGAGCAGG AGATTTCCAA AGTGCAGCGT AAGATGTCAG ACTTGGAGTC AGTTTTGCAG 
CAGAAAGACG TGGAGCTCAA GGCTTCAGAA ACCCAGAGAA ACATCTTAGA GCAAGACCTG 
GCAACCTATA TCACCGAATG CAGCAGTTTG AAGCGCAGTT TGGAGCAGGC CCGCATGGAG 
GTTTCGCAGG AAGATGATAA AGCTCTTCAG CTGCTGCACG ACATTCGAGA GCAGAGCAAC 
AAACTGCAGG AAATCAAAGA ACAGGAGTAC CACGCTCAGC TGGAGGAGAT GCGAGTGTCT 
ATTCGTCAGC TGGAAGAGAA TCTGTCGGCC GCTCGTCGCC GCAGTGACCT GTACGAGTCT 
GAGCTCAAAG AGTCCCGTCA GACCAGTGAG GAGCTCAAGA GGAAGGCAGT GGAGTATCAG 
CAGAAGATCC AGAAGGCTAA AGAACAAGGG AAAGTTGAGA TGGAGGAGCT TCTGAGCAAA 
CTGCAGAAGA CCAATGGAGA GCAACAAGTA AAGATTGAAG AGCTCCAGGA GAAGCTTGCT 
AAGGCAGTGA AGGCGAGTAC TGAAGCCACT GAACTCCTCC AGAATGTCCG TCAGGCTAAA 
GAGCGAATGG AGCGAGACCT GGAGCGTCTG CAGACCAAAG AGGACTCGTC CGACAGCCTG 
CGCAGACGCT TACGAGAGAC TGAGGATGGC AAAAAATCCC TGGAGAACCA GGTGAAGAGG 
CTGGAGATGG TGGAGCGGAG AGAATCCAAA CTGAAAGACG ACATTCAGAC CAAATCGCAG 
CAGATCCAGC AGATGGCCGA CAAAATCATG GAGCTTGAAG AAAATTTGCG GGACTCGCAG 
GCGATGGCGC AGCGCATGGA AACACAGCTG GAGCAGAAGG AAAAGCTTTT TGAAGACAAG 
ATCAAGGTTT TAGAAACTCA AATGAAGGCT GATTTGGCTG AGAAGGATTT GCTGGAGTCC 
AAACAGAGCA CTTTTGAAGA GGAGTCTCGC GAACGGGGAA AAGTGATCAG CGACCAGGCA 
GCGACGATCA ATGCCATGGA GTCAAAGATG ACCAGTCTGG AGCAGAGGAT CGCTGAGCTG 
TCCGAAGCCA ACAAACTGGC AGCAAACAGC AGCATTTACA CACAGAAGAA CATGAAAGCC 
CAGGAGGAGA TGATTTCTGA ACTCAGACAG CAGAAGTTTT ATTTGGAGTC TCAGGCTGGA 
AAACTGGAGG CGCAGAATGC AAAGCTGGAG GAGCATCTGG AGAAACTCAC CCAACAAGAG 
CAGACAAACA AGAGCCGAGT GCTGGAGCTG GAAGCCAGGC TGCGAGAGAT CGGCCTAGAG 
CATGAGGAAC AGAAGCTGGA GATCAAGCGT CAGGTGTCGG AGCTCACGTT GTCTCTGCAG 
GAGAGAGAAG CTCAGATCAG CAGCCTGCAA GCGGCACGCC ACGCTCTCGA CAGCCAACTA 
CAGCAGGCCA AAACTGAGCT GGAGGAGACC ACCGCCGAGG CCGAAGAGGA GATCACCGTC 
CTGAGGGCCC ATCGGGATGA AATCCAACGC AAGTTTGATG CCCTGCGAGA CAGCTGTGCG 
GTGATCACGG ATCTGGAGGA GCAGTTGACG CAGCTCACGC AAGAAAACGC GGAGCTGAAC 
CGGCAGAACT TCTACCTGTC CAAACAGCTG GACGAGGCCA CAGACGAGAA CGAGGAGCGC 
CTGCAGCTCA AGCAGGACGT GGAGCGCCTC AGGAGAGAGG TGGCAGACCG AGAGATGCAC 
CTCAACAACC AGAAACAGGT GACACGGCAA CACACACACA CACACACACA CACACACACA 
CACAGGACAG AGGTGGCAGA CCGAGAGATG CACCTCAACA ACCAGAAACA GAACATCGAG 
ACGCTGAAGA CGACATGTAC AATGCTGGAG GAGCAGGTTA TGGAGCTGGA GACTCTGAAT 
GATGAGCTGA TGGAGAAGGA GAGGCAGTGG GAGAACTGGA GATCTGCTCT GGACGAGGAG 
AAAAACCAGG CCGAGCGCAG AGCCAGAGAC CTGCAAAGAC AGCTGGACAG CGAGAAGCAG 
AACCGACTGC GTGCAGAACA GAGAAACTCT GAGTCTCGGC AGGCCGTGGA GCTGGCGGTG 
AAAGAGCACA AAGCTGAAAT CCTGGTTCTG CAGCAGGCGC TCAAAGACCA GAAACTCAAG 
GCTGAGGGCC TTTCTGACAC TCTGGGTGAT CTGGAGAAGA AGCACGGCAT GCTGGAGATG 
AACGCTCGCA GCCTGCAGCA GAAGCTGGAG ACGGAGAGAG AGATGAAGCA GAGACTCATG 
GACGAGCAAG CTAAACTGCA GCAGCAGCTG GACATGCAGA AGAGCCACAT CTTCAGACTG 
ACTCAAGGAC TGCAGGATGC GCTGGACCAG ACCGACCTGC TGAAGACCGA GCGCACAGAC 
CTGGAGTACC AGCTGGAAAA CATACAGGCG GCGTATTCTC ACGAGAAGGT GAAGATGGAG 
GGGACGATCA CCCAGCAGAC CAAGCTCATA GACTTCCTGC AGGCCAAGGT AGAGCACCCC 
TCTAAGAAGA AAAAGGGGCT GTTTGGTGGC CGGCGGCGGG AGGATCTGCT AGCGGCGCTG 
GCGGCTCAGG GACAGACACA GGTGCCTGTG GTGCCCGCCG CCACTCCTGT GCCCCTGCAG 
TACAGCGACC TGAAGGCGGC GCTGGATAAA GAGCGAGCCC GATGCTCCGA GCTGGAGGAC 
GCCCTGCAGA AAACACGCCT GGAGCTCAGA GAAGTTCAGT TTAATAAAGC TCTGGAGCAC 
AGCTCTTCTC CAGCCACTCC AGGTTTGGCC CGTCAGCAGC TCATGATGTC CACCAAGTCC 
AACAAGTCTC CAGAGCACCA GTCCAGCGGC GGCCTGATGT CCTCAAACTC CAGCCGCGGA 
CGCAAAGACA ACGCCAATCC TGAAGAAATA AGGAGAGTCA CTTCTGAAAA GTACACCAGA 
CGTGTGAAGG ACCGGATTCA TCACAACACT CCTCACCGCT TCACGCAGGG GCTCAATATG 
AGAGCGGCTC GCTGTACTGT GTGTCTGGAC ACAGTCCATT TTGGACGTCA GGCTGCAACA 
TGCATTGAAT GTCACGCGTT GTGTCACCCA AAATGCTCCC CGTACCTCCC TGCCACCTGC 
GGCGTTCCCA CTGAATACGC GCTGCACCTC TCAGAGGCTC TGTGTCGGGA GAAGGGAAGC 
ACTTCCGGCC TGAAAGATAT GGGTGGACAT ATGAGGCTTG AAGGATGGCT GAAGCAGCCC 
AGGAACGGGA AGCGCGGGCA GGGCTGGGAG AGGAAGTATG TGGTGCTGGA TGGATATAAA 
GTGATTACAT TCGAGACAGA ACCAAGAGAC GAGTCAGTGA AGCCGCTGGA TGAGTTTGAG 
CTGTGTCTCT CTGATGGAGA AGTGACCGTT CATTCTGCTG TGGGTTCCTC TGAGCTCCCC 
AACACCGCTA AAACAGATGT GCCGTACGTG CTGAAGCTGG AGTCGTCTCC TCAGTCCACA 
TGCTGGCCCG GTCAGACTCT GTACCTCATG ACCTCGGGAT ACTCAGAGAA GCAGCGCTGG 
GTGGCAGTGC TGGAAGCCAT CGTGGCCAGC AGTCGCGGAG CCAGAGAGAA AGCTGAGGCA 
GATGCTAAAT TATTGGGTAA CTCTTTACTT AAGCTAGAAG GTGACGACCG TCTGGATATC 
AACTGCACCC TGCCGCTGAC CGACCAGATT GTGTTGGTGG GCTCTGAGGA GGGTCTCTAC 
GCACTGAATG TGATTAAGAA CTCCCTCACT CATATCCCGG GTCTGGGATC CGTGTTTCAG 
ATCCACATTA TCAAAGATCA TGACAAGCTG CTGATGATCG TGGGAGACGA GCGAGCGCTG 
TGTCTGGTGG AGATTAAGAA GGTGAAGCAG TCTTTATCCC AGTCTCACCT CCCCACACTG 
CCCGAGATTG TGCCGTATAT CTTTGAGACC GTCAAGGGCT GTCATCTGTT TGCCTCGGGC 
AGGATAGAAA ATGGCCCATG CATTTGTGCT GCGATGCCCA ATAAAGTCAC GATTCTACGA 
TACAATGACA ATCTTAACAA GTTCTGCATA CGGAAGGAGA TTGAAACTCT GGAGCCGTGC 
AGCTGCATCC ACTTCACCAG CTACAGCATC ATCATTGGTA CCAATAAATT CTACGAGATC 
GAGATGAAGC AGTGCGTTCT GGACGAGTTC CTGGACAAGA ATGACGTGTC GCTGGCGTCG 
GCTGTCTTCA CCGGATCCTC CCACAGTTTC CCCATCTCTA TCGTCCAGGT GTCCAGCACT 
CCGCAGAAGG AGGAATATCT GCTGTGTTTC CACGAGATCG GAGTTTTTGT GGATGCTTAC 
GGTCGAAGAA GTCGCCCTGA TGATCTGAAG TGGAGTCGAC TTCCCCTGAC GTTCGCCTTC 
AGAGAGCCGT ATCTCTTTGT GACCTACTTC AACTCTCTGG ATGTTATTGA GATTCAGGGA 
CACGCAGCTC TTGGGCCTCC CAGTCTGGTT CACCTGGACA TTCCTAACCC ACGTTATCTG 
GGTCCAGCCA TATCGTCTGG TGCCATCTAT CTGGCCTCGT CCTACCAGAA CAAACTGCGG 
GTGATCTGCT GTAAGGGCAG TCTGGTGCGG GAGTCTGGAG AGCTGCAGAG GACCGGCTCC 
AGCAGGAGCC CCAACAAGCG CGGTCCACCA ACATACAACG AGCACATCTC CAAACGTCTG 
GCCTCGAGTC CTGGAGCCCA GGAGGGGCCC CTGCACCGCG AGCCCAGCAC TCCTCACCGC 
TACCGCGAGG GCCGCACCGA GTTCCGGAGA GACAAATCTC CAGCCCGGCC CCTGGAGCGA 
GAGAAATCCC CGGGCCGTCC GGTGCTAGAC AGCCGCAGAG AGAGGTCTCC CGGTCGCTTC 
GAGAGCAGCG GCAGCGAGAG AGACCGCGAG CGGACACGAC TGCACACTAG CTCTGTACGC 
ACCCAGCTGA CCCCCGTCAA CAAGGTACGC ATGAGACACT GCCACGTAAA CTGTGTGTCC 
TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100333880 ( XM_021478359.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334034.1 Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X4, mRNA.

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CloneID	ODa143751
Clone ID Related Accession (Same CDS sequence)	XM_021478359.1
Accession Version	XM_021478359.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	citron Rho-interacting kinase-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG AGATTTCCAA AGTGCAGCGT AAGATGTCAG ACTTGGAGTC AGTTTTGCAG 
CAGAAAGACG TGGAGCTCAA GGCTTCAGAA ACCCAGAGAA ACATCTTAGA GCAAGACCTG 
GCAACCTATA TCACCGAATG CAGCAGTTTG AAGCGCAGTT TGGAGCAGGC CCGCATGGAG 
GTTTCGCAGG AAGATGATAA AGCTCTTCAG CTGCTGCACG ACATTCGAGA GCAGAGCAAC 
AAACTGCAGG AAATCAAAGA ACAGGAGTAC CACGCTCAGC TGGAGGAGAT GCGAGTGTCT 
ATTCGTCAGC TGGAAGAGAA TCTGTCGGCC GCTCGTCGCC GCAGTGACCT GTACGAGTCT 
GAGCTCAAAG AGTCCCGTCA GACCAGTGAG GAGCTCAAGA GGAAGGCAGT GGAGTATCAG 
CAGAAGATCC AGAAGGCTAA AGAACAAGGG AAAGTTGAGA TGGAGGAGCT TCTGAGCAAA 
CTGCAGAAGA CCAATGGAGA GCAACAAGTA AAGATTGAAG AGCTCCAGGA GAAGCTTGCT 
AAGGCAGTGA AGGCGAGTAC TGAAGCCACT GAACTCCTCC AGAATGTCCG TCAGGCTAAA 
GAGCGAATGG AGCGAGACCT GGAGCGTCTG CAGACCAAAG AGGACTCGTC CGACAGCCTG 
CGCAGACGCT TACGAGAGAC TGAGGATGGC AAAAAATCCC TGGAGAACCA GGTGAAGAGG 
CTGGAGATGG TGGAGCGGAG AGAATCCAAA CTGAAAGACG ACATTCAGAC CAAATCGCAG 
CAGATCCAGC AGATGGCCGA CAAAATCATG GAGCTTGAAG AAAATTTGCG GGACTCGCAG 
GCGATGGCGC AGCGCATGGA AACACAGCTG GAGCAGAAGG AAAAGCTTTT TGAAGACAAG 
ATCAAGGTTT TAGAAACTCA AATGAAGGCT GATTTGGCTG AGAAGGATTT GCTGGAGTCC 
AAACAGAGCA CTTTTGAAGA GGAGTCTCGC GAACGGGGAA AAGTGATCAG CGACCAGGCA 
GCGACGATCA ATGCCATGGA GTCAAAGATG ACCAGTCTGG AGCAGAGGAT CGCTGAGCTG 
TCCGAAGCCA ACAAACTGGC AGCAAACAGC AGCATTTACA CACAGAAGAA CATGAAAGCC 
CAGGAGGAGA TGATTTCTGA ACTCAGACAG CAGAAGTTTT ATTTGGAGTC TCAGGCTGGA 
AAACTGGAGG CGCAGAATGC AAAGCTGGAG GAGCATCTGG AGAAACTCAC CCAACAAGAG 
CAGACAAACA AGAGCCGAGT GCTGGAGCTG GAAGCCAGGC TGCGAGAGAT CGGCCTAGAG 
CATGAGGAAC AGAAGCTGGA GATCAAGCGT CAGGTGTCGG AGCTCACGTT GTCTCTGCAG 
GAGAGAGAAG CTCAGATCAG CAGCCTGCAA GCGGCACGCC ACGCTCTCGA CAGCCAACTA 
CAGCAGGCCA AAACTGAGCT GGAGGAGACC ACCGCCGAGG CCGAAGAGGA GATCACCGTC 
CTGAGGGCCC ATCGGGATGA AATCCAACGC AAGTTTGATG CCCTGCGAGA CAGCTGTGCG 
GTGATCACGG ATCTGGAGGA GCAGTTGACG CAGCTCACGC AAGAAAACGC GGAGCTGAAC 
CGGCAGAACT TCTACCTGTC CAAACAGCTG GACGAGGCCA CAGACGAGAA CGAGGAGCGC 
CTGCAGCTCA AGCAGGACGT GGAGCGCCTC AGGAGAGAGG TGGCAGACCG AGAGATGCAC 
CTCAACAACC AGAAACAGGT GACACGGCAA CACACACACA CACACACACA CACACACACA 
CACAGGACAG AGGTGGCAGA CCGAGAGATG CACCTCAACA ACCAGAAACA GAACATCGAG 
ACGCTGAAGA CGACATGTAC AATGCTGGAG GAGCAGGTTA TGGAGCTGGA GACTCTGAAT 
GATGAGCTGA TGGAGAAGGA GAGGCAGTGG GAGAACTGGA GATCTGCTCT GGACGAGGAG 
AAAAACCAGG CCGAGCGCAG AGCCAGAGAC CTGCAAAGAC AGCTGGACAG CGAGAAGCAG 
AACCGACTGC GTGCAGAACA GAGAAACTCT GAGTCTCGGC AGGCCGTGGA GCTGGCGGTG 
AAAGAGCACA AAGCTGAAAT CCTGGTTCTG CAGCAGGCGC TCAAAGACCA GAAACTCAAG 
GCTGAGGGCC TTTCTGACAC TCTGGGTGAT CTGGAGAAGA AGCACGGCAT GCTGGAGATG 
AACGCTCGCA GCCTGCAGCA GAAGCTGGAG ACGGAGAGAG AGATGAAGCA GAGACTCATG 
GACGAGCAAG CTAAACTGCA GCAGCAGCTG GACATGCAGA AGAGCCACAT CTTCAGACTG 
ACTCAAGGAC TGCAGGATGC GCTGGACCAG ACCGACCTGC TGAAGACCGA GCGCACAGAC 
CTGGAGTACC AGCTGGAAAA CATACAGGCG GCGTATTCTC ACGAGAAGGT GAAGATGGAG 
GGGACGATCA CCCAGCAGAC CAAGCTCATA GACTTCCTGC AGGCCAAGGT AGAGCACCCC 
TCTAAGAAGA AAAAGGGGCT GTTTGGTGGC CGGCGGCGGG AGGATCTGCT AGCGGCGCTG 
GCGGCTCAGG GACAGACACA GGTGCCTGTG GTGCCCGCCG CCACTCCTGT GCCCCTGCAG 
TACAGCGACC TGAAGGCGGC GCTGGATAAA GAGCGAGCCC GATGCTCCGA GCTGGAGGAC 
GCCCTGCAGA AAACACGCCT GGAGCTCAGA GAAGTTCAGT TTAATAAAGC TCTGGAGCAC 
AGCTCTTCTC CAGCCACTCC AGGTTTGGCC CGTCAGCAGC TCATGATGTC CACCAAGTCC 
AACAAGTCTC CAGAGCACCA GTCCAGCGGC GGCCTGATGT CCTCAAACTC CAGCCGCGGA 
CGCAAAGACA ACGCCAATCC TGAAGAGTAC ACCAGACGTG TGAAGGACCG GATTCATCAC 
AACACTCCTC ACCGCTTCAC GCAGGGGCTC AATATGAGAG CGGCTCGCTG TACTGTGTGT 
CTGGACACAG TCCATTTTGG ACGTCAGGCT GCAACATGCA TTGAATGTCA CGCGTTGTGT 
CACCCAAAAT GCTCCCCGTA CCTCCCTGCC ACCTGCGGCG TTCCCACTGA ATACGCGCTG 
CACCTCTCAG AGGCTCTGTG TCGGGAGAAG GGAAGCACTT CCGGCCTGAA AGATATGGGT 
GGACATATGA GGCTTGAAGG ATGGCTGAAG CAGCCCAGGA ACGGGAAGCG CGGGCAGGGC 
TGGGAGAGGA AGTATGTGGT GCTGGATGGA TATAAAGTGA TTACATTCGA GACAGAACCA 
AGAGACGAGT CAGTGAAGCC GCTGGATGAG TTTGAGCTGT GTCTCTCTGA TGGAGAAGTG 
ACCGTTCATT CTGCTGTGGG TTCCTCTGAG CTCCCCAACA CCGCTAAAAC AGATGTGCCG 
TACGTGCTGA AGCTGGAGTC GTCTCCTCAG TCCACATGCT GGCCCGGTCA GACTCTGTAC 
CTCATGACCT CGGGATACTC AGAGAAGCAG CGCTGGGTGG CAGTGCTGGA AGCCATCGTG 
GCCAGCAGTC GCGGAGCCAG AGAGAAAGCT GAGGCAGATG CTAAATTATT GGGTAACTCT 
TTACTTAAGC TAGAAGGTGA CGACCGTCTG GATATCAACT GCACCCTGCC GCTGACCGAC 
CAGATTGTGT TGGTGGGCTC TGAGGAGGGT CTCTACGCAC TGAATGTGAT TAAGAACTCC 
CTCACTCATA TCCCGGGTCT GGGATCCGTG TTTCAGATCC ACATTATCAA AGATCATGAC 
AAGCTGCTGA TGATCGTGGG AGACGAGCGA GCGCTGTGTC TGGTGGAGAT TAAGAAGGTG 
AAGCAGTCTT TATCCCAGTC TCACCTCCCC ACACTGCCCG AGATTGTGCC GTATATCTTT 
GAGACCGTCA AGGGCTGTCA TCTGTTTGCC TCGGGCAGGA TAGAAAATGG CCCATGCATT 
TGTGCTGCGA TGCCCAATAA AGTCACGATT CTACGATACA ATGACAATCT TAACAAGTTC 
TGCATACGGA AGGAGATTGA AACTCTGGAG CCGTGCAGCT GCATCCACTT CACCAGCTAC 
AGCATCATCA TTGGTACCAA TAAATTCTAC GAGATCGAGA TGAAGCAGTG CGTTCTGGAC 
GAGTTCCTGG ACAAGAATGA CGTGTCGCTG GCGTCGGCTG TCTTCACCGG ATCCTCCCAC 
AGTTTCCCCA TCTCTATCGT CCAGGTGTCC AGCACTCCGC AGAAGGAGGA ATATCTGCTG 
TGTTTCCACG AGATCGGAGT TTTTGTGGAT GCTTACGGTC GAAGAAGTCG CCCTGATGAT 
CTGAAGTGGA GTCGACTTCC CCTGACGTTC GCCTTCAGAG AGCCGTATCT CTTTGTGACC 
TACTTCAACT CTCTGGATGT TATTGAGATT CAGGGACACG CAGCTCTTGG GCCTCCCAGT 
CTGGTTCACC TGGACATTCC TAACCCACGT TATCTGGGTC CAGCCATATC GTCTGGTGCC 
ATCTATCTGG CCTCGTCCTA CCAGAACAAA CTGCGGGTGA TCTGCTGTAA GGGCAGTCTG 
GTGCGGGAGT CTGGAGAGCT GCAGAGGACC GGCTCCAGCA GGAGCAGCCC CAACAAGCGC 
GGTCCACCAA CATACAACGA GCACATCTCC AAACGTCTGG CCTCGAGTCC TGGAGCCCAG 
GAGGGGCCCC TGCACCGCGA GCCCAGCACT CCTCACCGCT ACCGCGAGGG CCGCACCGAG 
TTCCGGAGAG ACAAATCTCC AGCCCGGCCC CTGGAGCGAG AGAAATCCCC GGGCCGTCCG 
GTGCTAGACA GCCGCAGAGA GAGGTCTCCC GGTCGCTTCG AGAGCAGCGG CAGCGAGAGA 
GACCGCGAGC GGACACGACT GCACACTAGC TCTGTACGCA CCCAGCTGAC CCCCGTCAAC 
AAGGTACGCA TGAGACACTG CCACGTAAAC TGTGTGTCCT TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334034.1
CDS	433..5397
Translation	MEQEISKVQRKMSDLESVLQQKDVELKASETQRNILEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSNKLQEIKEQEYHAQLEEMRVSIRQLEENLSAARRRSDLYESELKESRQTSEELKRKAVEYQQKIQKAKEQGKVEMEELLSKLQKTNGEQQVKIEELQEKLAKAVKASTEATELLQNVRQAKERMERDLERLQTKEDSSDSLRRRLRETEDGKKSLENQVKRLEMVERRESKLKDDIQTKSQQIQQMADKIMELEENLRDSQAMAQRMETQLEQKEKLFEDKIKVLETQMKADLAEKDLLESKQSTFEEESRERGKVISDQAATINAMESKMTSLEQRIAELSEANKLAANSSIYTQKNMKAQEEMISELRQQKFYLESQAGKLEAQNAKLEEHLEKLTQQEQTNKSRVLELEARLREIGLEHEEQKLEIKRQVSELTLSLQEREAQISSLQAARHALDSQLQQAKTELEETTAEAEEEITVLRAHRDEIQRKFDALRDSCAVITDLEEQLTQLTQENAELNRQNFYLSKQLDEATDENEERLQLKQDVERLRREVADREMHLNNQKQVTRQHTHTHTHTHTHRTEVADREMHLNNQKQNIETLKTTCTMLEEQVMELETLNDELMEKERQWENWRSALDEEKNQAERRARDLQRQLDSEKQNRLRAEQRNSESRQAVELAVKEHKAEILVLQQALKDQKLKAEGLSDTLGDLEKKHGMLEMNARSLQQKLETEREMKQRLMDEQAKLQQQLDMQKSHIFRLTQGLQDALDQTDLLKTERTDLEYQLENIQAAYSHEKVKMEGTITQQTKLIDFLQAKVEHPSKKKKGLFGGRRREDLLAALAAQGQTQVPVVPAATPVPLQYSDLKAALDKERARCSELEDALQKTRLELREVQFNKALEHSSSPATPGLARQQLMMSTKSNKSPEHQSSGGLMSSNSSRGRKDNANPEEYTRRVKDRIHHNTPHRFTQGLNMRAARCTVCLDTVHFGRQAATCIECHALCHPKCSPYLPATCGVPTEYALHLSEALCREKGSTSGLKDMGGHMRLEGWLKQPRNGKRGQGWERKYVVLDGYKVITFETEPRDESVKPLDEFELCLSDGEVTVHSAVGSSELPNTAKTDVPYVLKLESSPQSTCWPGQTLYLMTSGYSEKQRWVAVLEAIVASSRGAREKAEADAKLLGNSLLKLEGDDRLDINCTLPLTDQIVLVGSEEGLYALNVIKNSLTHIPGLGSVFQIHIIKDHDKLLMIVGDERALCLVEIKKVKQSLSQSHLPTLPEIVPYIFETVKGCHLFASGRIENGPCICAAMPNKVTILRYNDNLNKFCIRKEIETLEPCSCIHFTSYSIIIGTNKFYEIEMKQCVLDEFLDKNDVSLASAVFTGSSHSFPISIVQVSSTPQKEEYLLCFHEIGVFVDAYGRRSRPDDLKWSRLPLTFAFREPYLFVTYFNSLDVIEIQGHAALGPPSLVHLDIPNPRYLGPAISSGAIYLASSYQNKLRVICCKGSLVRESGELQRTGSSRSSPNKRGPPTYNEHISKRLASSPGAQEGPLHREPSTPHRYREGRTEFRRDKSPARPLEREKSPGRPVLDSRRERSPGRFESSGSERDRERTRLHTSSVRTQLTPVNKVRMRHCHVNCVSF

Target ORF information:

RefSeq Version	XM_021478359.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478359.1

ORF Insert Sequence:

ATGGAGCAGG AGATTTCCAA AGTGCAGCGT AAGATGTCAG ACTTGGAGTC AGTTTTGCAG 
CAGAAAGACG TGGAGCTCAA GGCTTCAGAA ACCCAGAGAA ACATCTTAGA GCAAGACCTG 
GCAACCTATA TCACCGAATG CAGCAGTTTG AAGCGCAGTT TGGAGCAGGC CCGCATGGAG 
GTTTCGCAGG AAGATGATAA AGCTCTTCAG CTGCTGCACG ACATTCGAGA GCAGAGCAAC 
AAACTGCAGG AAATCAAAGA ACAGGAGTAC CACGCTCAGC TGGAGGAGAT GCGAGTGTCT 
ATTCGTCAGC TGGAAGAGAA TCTGTCGGCC GCTCGTCGCC GCAGTGACCT GTACGAGTCT 
GAGCTCAAAG AGTCCCGTCA GACCAGTGAG GAGCTCAAGA GGAAGGCAGT GGAGTATCAG 
CAGAAGATCC AGAAGGCTAA AGAACAAGGG AAAGTTGAGA TGGAGGAGCT TCTGAGCAAA 
CTGCAGAAGA CCAATGGAGA GCAACAAGTA AAGATTGAAG AGCTCCAGGA GAAGCTTGCT 
AAGGCAGTGA AGGCGAGTAC TGAAGCCACT GAACTCCTCC AGAATGTCCG TCAGGCTAAA 
GAGCGAATGG AGCGAGACCT GGAGCGTCTG CAGACCAAAG AGGACTCGTC CGACAGCCTG 
CGCAGACGCT TACGAGAGAC TGAGGATGGC AAAAAATCCC TGGAGAACCA GGTGAAGAGG 
CTGGAGATGG TGGAGCGGAG AGAATCCAAA CTGAAAGACG ACATTCAGAC CAAATCGCAG 
CAGATCCAGC AGATGGCCGA CAAAATCATG GAGCTTGAAG AAAATTTGCG GGACTCGCAG 
GCGATGGCGC AGCGCATGGA AACACAGCTG GAGCAGAAGG AAAAGCTTTT TGAAGACAAG 
ATCAAGGTTT TAGAAACTCA AATGAAGGCT GATTTGGCTG AGAAGGATTT GCTGGAGTCC 
AAACAGAGCA CTTTTGAAGA GGAGTCTCGC GAACGGGGAA AAGTGATCAG CGACCAGGCA 
GCGACGATCA ATGCCATGGA GTCAAAGATG ACCAGTCTGG AGCAGAGGAT CGCTGAGCTG 
TCCGAAGCCA ACAAACTGGC AGCAAACAGC AGCATTTACA CACAGAAGAA CATGAAAGCC 
CAGGAGGAGA TGATTTCTGA ACTCAGACAG CAGAAGTTTT ATTTGGAGTC TCAGGCTGGA 
AAACTGGAGG CGCAGAATGC AAAGCTGGAG GAGCATCTGG AGAAACTCAC CCAACAAGAG 
CAGACAAACA AGAGCCGAGT GCTGGAGCTG GAAGCCAGGC TGCGAGAGAT CGGCCTAGAG 
CATGAGGAAC AGAAGCTGGA GATCAAGCGT CAGGTGTCGG AGCTCACGTT GTCTCTGCAG 
GAGAGAGAAG CTCAGATCAG CAGCCTGCAA GCGGCACGCC ACGCTCTCGA CAGCCAACTA 
CAGCAGGCCA AAACTGAGCT GGAGGAGACC ACCGCCGAGG CCGAAGAGGA GATCACCGTC 
CTGAGGGCCC ATCGGGATGA AATCCAACGC AAGTTTGATG CCCTGCGAGA CAGCTGTGCG 
GTGATCACGG ATCTGGAGGA GCAGTTGACG CAGCTCACGC AAGAAAACGC GGAGCTGAAC 
CGGCAGAACT TCTACCTGTC CAAACAGCTG GACGAGGCCA CAGACGAGAA CGAGGAGCGC 
CTGCAGCTCA AGCAGGACGT GGAGCGCCTC AGGAGAGAGG TGGCAGACCG AGAGATGCAC 
CTCAACAACC AGAAACAGGT GACACGGCAA CACACACACA CACACACACA CACACACACA 
CACAGGACAG AGGTGGCAGA CCGAGAGATG CACCTCAACA ACCAGAAACA GAACATCGAG 
ACGCTGAAGA CGACATGTAC AATGCTGGAG GAGCAGGTTA TGGAGCTGGA GACTCTGAAT 
GATGAGCTGA TGGAGAAGGA GAGGCAGTGG GAGAACTGGA GATCTGCTCT GGACGAGGAG 
AAAAACCAGG CCGAGCGCAG AGCCAGAGAC CTGCAAAGAC AGCTGGACAG CGAGAAGCAG 
AACCGACTGC GTGCAGAACA GAGAAACTCT GAGTCTCGGC AGGCCGTGGA GCTGGCGGTG 
AAAGAGCACA AAGCTGAAAT CCTGGTTCTG CAGCAGGCGC TCAAAGACCA GAAACTCAAG 
GCTGAGGGCC TTTCTGACAC TCTGGGTGAT CTGGAGAAGA AGCACGGCAT GCTGGAGATG 
AACGCTCGCA GCCTGCAGCA GAAGCTGGAG ACGGAGAGAG AGATGAAGCA GAGACTCATG 
GACGAGCAAG CTAAACTGCA GCAGCAGCTG GACATGCAGA AGAGCCACAT CTTCAGACTG 
ACTCAAGGAC TGCAGGATGC GCTGGACCAG ACCGACCTGC TGAAGACCGA GCGCACAGAC 
CTGGAGTACC AGCTGGAAAA CATACAGGCG GCGTATTCTC ACGAGAAGGT GAAGATGGAG 
GGGACGATCA CCCAGCAGAC CAAGCTCATA GACTTCCTGC AGGCCAAGGT AGAGCACCCC 
TCTAAGAAGA AAAAGGGGCT GTTTGGTGGC CGGCGGCGGG AGGATCTGCT AGCGGCGCTG 
GCGGCTCAGG GACAGACACA GGTGCCTGTG GTGCCCGCCG CCACTCCTGT GCCCCTGCAG 
TACAGCGACC TGAAGGCGGC GCTGGATAAA GAGCGAGCCC GATGCTCCGA GCTGGAGGAC 
GCCCTGCAGA AAACACGCCT GGAGCTCAGA GAAGTTCAGT TTAATAAAGC TCTGGAGCAC 
AGCTCTTCTC CAGCCACTCC AGGTTTGGCC CGTCAGCAGC TCATGATGTC CACCAAGTCC 
AACAAGTCTC CAGAGCACCA GTCCAGCGGC GGCCTGATGT CCTCAAACTC CAGCCGCGGA 
CGCAAAGACA ACGCCAATCC TGAAGAGTAC ACCAGACGTG TGAAGGACCG GATTCATCAC 
AACACTCCTC ACCGCTTCAC GCAGGGGCTC AATATGAGAG CGGCTCGCTG TACTGTGTGT 
CTGGACACAG TCCATTTTGG ACGTCAGGCT GCAACATGCA TTGAATGTCA CGCGTTGTGT 
CACCCAAAAT GCTCCCCGTA CCTCCCTGCC ACCTGCGGCG TTCCCACTGA ATACGCGCTG 
CACCTCTCAG AGGCTCTGTG TCGGGAGAAG GGAAGCACTT CCGGCCTGAA AGATATGGGT 
GGACATATGA GGCTTGAAGG ATGGCTGAAG CAGCCCAGGA ACGGGAAGCG CGGGCAGGGC 
TGGGAGAGGA AGTATGTGGT GCTGGATGGA TATAAAGTGA TTACATTCGA GACAGAACCA 
AGAGACGAGT CAGTGAAGCC GCTGGATGAG TTTGAGCTGT GTCTCTCTGA TGGAGAAGTG 
ACCGTTCATT CTGCTGTGGG TTCCTCTGAG CTCCCCAACA CCGCTAAAAC AGATGTGCCG 
TACGTGCTGA AGCTGGAGTC GTCTCCTCAG TCCACATGCT GGCCCGGTCA GACTCTGTAC 
CTCATGACCT CGGGATACTC AGAGAAGCAG CGCTGGGTGG CAGTGCTGGA AGCCATCGTG 
GCCAGCAGTC GCGGAGCCAG AGAGAAAGCT GAGGCAGATG CTAAATTATT GGGTAACTCT 
TTACTTAAGC TAGAAGGTGA CGACCGTCTG GATATCAACT GCACCCTGCC GCTGACCGAC 
CAGATTGTGT TGGTGGGCTC TGAGGAGGGT CTCTACGCAC TGAATGTGAT TAAGAACTCC 
CTCACTCATA TCCCGGGTCT GGGATCCGTG TTTCAGATCC ACATTATCAA AGATCATGAC 
AAGCTGCTGA TGATCGTGGG AGACGAGCGA GCGCTGTGTC TGGTGGAGAT TAAGAAGGTG 
AAGCAGTCTT TATCCCAGTC TCACCTCCCC ACACTGCCCG AGATTGTGCC GTATATCTTT 
GAGACCGTCA AGGGCTGTCA TCTGTTTGCC TCGGGCAGGA TAGAAAATGG CCCATGCATT 
TGTGCTGCGA TGCCCAATAA AGTCACGATT CTACGATACA ATGACAATCT TAACAAGTTC 
TGCATACGGA AGGAGATTGA AACTCTGGAG CCGTGCAGCT GCATCCACTT CACCAGCTAC 
AGCATCATCA TTGGTACCAA TAAATTCTAC GAGATCGAGA TGAAGCAGTG CGTTCTGGAC 
GAGTTCCTGG ACAAGAATGA CGTGTCGCTG GCGTCGGCTG TCTTCACCGG ATCCTCCCAC 
AGTTTCCCCA TCTCTATCGT CCAGGTGTCC AGCACTCCGC AGAAGGAGGA ATATCTGCTG 
TGTTTCCACG AGATCGGAGT TTTTGTGGAT GCTTACGGTC GAAGAAGTCG CCCTGATGAT 
CTGAAGTGGA GTCGACTTCC CCTGACGTTC GCCTTCAGAG AGCCGTATCT CTTTGTGACC 
TACTTCAACT CTCTGGATGT TATTGAGATT CAGGGACACG CAGCTCTTGG GCCTCCCAGT 
CTGGTTCACC TGGACATTCC TAACCCACGT TATCTGGGTC CAGCCATATC GTCTGGTGCC 
ATCTATCTGG CCTCGTCCTA CCAGAACAAA CTGCGGGTGA TCTGCTGTAA GGGCAGTCTG 
GTGCGGGAGT CTGGAGAGCT GCAGAGGACC GGCTCCAGCA GGAGCAGCCC CAACAAGCGC 
GGTCCACCAA CATACAACGA GCACATCTCC AAACGTCTGG CCTCGAGTCC TGGAGCCCAG 
GAGGGGCCCC TGCACCGCGA GCCCAGCACT CCTCACCGCT ACCGCGAGGG CCGCACCGAG 
TTCCGGAGAG ACAAATCTCC AGCCCGGCCC CTGGAGCGAG AGAAATCCCC GGGCCGTCCG 
GTGCTAGACA GCCGCAGAGA GAGGTCTCCC GGTCGCTTCG AGAGCAGCGG CAGCGAGAGA 
GACCGCGAGC GGACACGACT GCACACTAGC TCTGTACGCA CCCAGCTGAC CCCCGTCAAC 
AAGGTACGCA TGAGACACTG CCACGTAAAC TGTGTGTCCT TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100333880 ( XM_021478360.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334035.1 Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X5, mRNA.

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CloneID	ODa143752
Clone ID Related Accession (Same CDS sequence)	XM_021478360.1
Accession Version	XM_021478360.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	citron Rho-interacting kinase-like isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG AGATTTCCAA AGTGCAGCGT AAGATGTCAG ACTTGGAGTC AGTTTTGCAG 
CAGAAAGACG TGGAGCTCAA GGCTTCAGAA ACCCAGAGAA ACATCTTAGA GCAAGACCTG 
GCAACCTATA TCACCGAATG CAGCAGTTTG AAGCGCAGTT TGGAGCAGGC CCGCATGGAG 
GTTTCGCAGG AAGATGATAA AGCTCTTCAG CTGCTGCACG ACATTCGAGA GCAGAGCAAC 
AAACTGCAGG AAATCAAAGA ACAGGAGTAC CACGCTCAGC TGGAGGAGAT GCGAGTGTCT 
ATTCGTCAGC TGGAAGAGAA TCTGTCGGCC GCTCGTCGCC GCAGTGACCT GTACGAGTCT 
GAGCTCAAAG AGTCCCGTCA GACCAGTGAG GAGCTCAAGA GGAAGGCAGT GGAGTATCAG 
CAGAAGATCC AGAAGGCTAA AGAACAAGGG AAAGTTGAGA TGGAGGAGCT TCTGAGCAAA 
CTGCAGAAGA CCAATGGAGA GCAACAAGTA AAGATTGAAG AGCTCCAGGA GAAGCTTGCT 
AAGGCAGTGA AGGCGAGTAC TGAAGCCACT GAACTCCTCC AGAATGTCCG TCAGGCTAAA 
GAGCGAATGG AGCGAGACCT GGAGCGTCTG CAGACCAAAG AGGACTCGTC CGACAGCCTG 
CGCAGACGCT TACGAGAGAC TGAGGATGGC AAAAAATCCC TGGAGAACCA GGTGAAGAGG 
CTGGAGATGG TGGAGCGGAG AGAATCCAAA CTGAAAGACG ACATTCAGAC CAAATCGCAG 
CAGATCCAGC AGATGGCCGA CAAAATCATG GAGCTTGAAG AAAATTTGCG GGACTCGCAG 
GCGATGGCGC AGCGCATGGA AACACAGCTG GAGCAGAAGG AAAAGCTTTT TGAAGACAAG 
ATCAAGGTTT TAGAAACTCA AATGAAGGCT GATTTGGCTG AGAAGGATTT GCTGGAGTCC 
AAACAGAGCA CTTTTGAAGA GGAGTCTCGC GAACGGGGAA AAGTGATCAG CGACCAGGCA 
GCGACGATCA ATGCCATGGA GTCAAAGATG ACCAGTCTGG AGCAGAGGAT CGCTGAGCTG 
TCCGAAGCCA ACAAACTGGC AGCAAACAGC AGCATTTACA CACAGAAGAA CATGAAAGCC 
CAGGAGGAGA TGATTTCTGA ACTCAGACAG CAGAAGTTTT ATTTGGAGTC TCAGGCTGGA 
AAACTGGAGG CGCAGAATGC AAAGCTGGAG GAGCATCTGG AGAAACTCAC CCAACAAGAG 
CAGACAAACA AGAGCCGAGT GCTGGAGCTG GAAGCCAGGC TGCGAGAGAT CGGCCTAGAG 
CATGAGGAAC AGAAGCTGGA GATCAAGCGT CAGGTGTCGG AGCTCACGTT GTCTCTGCAG 
GAGAGAGAAG CTCAGATCAG CAGCCTGCAA GCGGCACGCC ACGCTCTCGA CAGCCAACTA 
CAGCAGGCCA AAACTGAGCT GGAGGAGACC ACCGCCGAGG CCGAAGAGGA GATCACCGTC 
CTGAGGGCCC ATCGGGATGA AATCCAACGC AAGTTTGATG CCCTGCGAGA CAGCTGTGCG 
GTGATCACGG ATCTGGAGGA GCAGTTGACG CAGCTCACGC AAGAAAACGC GGAGCTGAAC 
CGGCAGAACT TCTACCTGTC CAAACAGCTG GACGAGGCCA CAGACGAGAA CGAGGAGCGC 
CTGCAGCTCA AGCAGGACGT GGAGCGCCTC AGGAGAGAGG TGGCAGACCG AGAGATGCAC 
CTCAACAACC AGAAACAGGT GACACGGCAA CACACACACA CACACACACA CACACACACA 
CACAGGACAG AGGTGGCAGA CCGAGAGATG CACCTCAACA ACCAGAAACA GAACATCGAG 
ACGCTGAAGA CGACATGTAC AATGCTGGAG GAGCAGGTTA TGGAGCTGGA GACTCTGAAT 
GATGAGCTGA TGGAGAAGGA GAGGCAGTGG GAGAACTGGA GATCTGCTCT GGACGAGGAG 
AAAAACCAGG CCGAGCGCAG AGCCAGAGAC CTGCAAAGAC AGCTGGACAG CGAGAAGCAG 
AACCGACTGC GTGCAGAACA GAGAAACTCT GAGTCTCGGC AGGCCGTGGA GCTGGCGGTG 
AAAGAGCACA AAGCTGAAAT CCTGGTTCTG CAGCAGGCGC TCAAAGACCA GAAACTCAAG 
GCTGAGGGCC TTTCTGACAC TCTGGGTGAT CTGGAGAAGA AGCACGGCAT GCTGGAGATG 
AACGCTCGCA GCCTGCAGCA GAAGCTGGAG ACGGAGAGAG AGATGAAGCA GAGACTCATG 
GACGAGCAAG CTAAACTGCA GCAGCAGCTG GACATGCAGA AGAGCCACAT CTTCAGACTG 
ACTCAAGGAC TGCAGGATGC GCTGGACCAG ACCGACCTGC TGAAGACCGA GCGCACAGAC 
CTGGAGTACC AGCTGGAAAA CATACAGGCG GCGTATTCTC ACGAGAAGGT GAAGATGGAG 
GGGACGATCA CCCAGCAGAC CAAGCTCATA GACTTCCTGC AGGCCAAGGG GCTGTTTGGT 
GGCCGGCGGC GGGAGGATCT GCTAGCGGCG CTGGCGGCTC AGGGACAGAC ACAGGTGCCT 
GTGGTGCCCG CCGCCACTCC TGTGCCCCTG CAGTACAGCG ACCTGAAGGC GGCGCTGGAT 
AAAGAGCGAG CCCGATGCTC CGAGCTGGAG GACGCCCTGC AGAAAACACG CCTGGAGCTC 
AGAGAAGTTC AGTTTAATAA AGCTCTGGAG CACAGCTCTT CTCCAGCCAC TCCAGGTTTG 
GCCCGTCAGC AGCTCATGAT GTCCACCAAG TCCAACAAGT CTCCAGAGCA CCAGTCCAGC 
GGCGGCCTGA TGTCCTCAAA CTCCAGCCGC GGACGCAAAG ACAACGCCAA TCCTGAAGAA 
ATAAGGAGAG TCACTTCTGA AAAGTACACC AGACGTGTGA AGGACCGGAT TCATCACAAC 
ACTCCTCACC GCTTCACGCA GGGGCTCAAT ATGAGAGCGG CTCGCTGTAC TGTGTGTCTG 
GACACAGTCC ATTTTGGACG TCAGGCTGCA ACATGCATTG AATGTCACGC GTTGTGTCAC 
CCAAAATGCT CCCCGTACCT CCCTGCCACC TGCGGCGTTC CCACTGAATA CGCGCTGCAC 
CTCTCAGAGG CTCTGTGTCG GGAGAAGGGA AGCACTTCCG GCCTGAAAGA TATGGGTGGA 
CATATGAGGC TTGAAGGATG GCTGAAGCAG CCCAGGAACG GGAAGCGCGG GCAGGGCTGG 
GAGAGGAAGT ATGTGGTGCT GGATGGATAT AAAGTGATTA CATTCGAGAC AGAACCAAGA 
GACGAGTCAG TGAAGCCGCT GGATGAGTTT GAGCTGTGTC TCTCTGATGG AGAAGTGACC 
GTTCATTCTG CTGTGGGTTC CTCTGAGCTC CCCAACACCG CTAAAACAGA TGTGCCGTAC 
GTGCTGAAGC TGGAGTCGTC TCCTCAGTCC ACATGCTGGC CCGGTCAGAC TCTGTACCTC 
ATGACCTCGG GATACTCAGA GAAGCAGCGC TGGGTGGCAG TGCTGGAAGC CATCGTGGCC 
AGCAGTCGCG GAGCCAGAGA GAAAGCTGAG GCAGATGCTA AATTATTGGG TAACTCTTTA 
CTTAAGCTAG AAGGTGACGA CCGTCTGGAT ATCAACTGCA CCCTGCCGCT GACCGACCAG 
ATTGTGTTGG TGGGCTCTGA GGAGGGTCTC TACGCACTGA ATGTGATTAA GAACTCCCTC 
ACTCATATCC CGGGTCTGGG ATCCGTGTTT CAGATCCACA TTATCAAAGA TCATGACAAG 
CTGCTGATGA TCGTGGGAGA CGAGCGAGCG CTGTGTCTGG TGGAGATTAA GAAGGTGAAG 
CAGTCTTTAT CCCAGTCTCA CCTCCCCACA CTGCCCGAGA TTGTGCCGTA TATCTTTGAG 
ACCGTCAAGG GCTGTCATCT GTTTGCCTCG GGCAGGATAG AAAATGGCCC ATGCATTTGT 
GCTGCGATGC CCAATAAAGT CACGATTCTA CGATACAATG ACAATCTTAA CAAGTTCTGC 
ATACGGAAGG AGATTGAAAC TCTGGAGCCG TGCAGCTGCA TCCACTTCAC CAGCTACAGC 
ATCATCATTG GTACCAATAA ATTCTACGAG ATCGAGATGA AGCAGTGCGT TCTGGACGAG 
TTCCTGGACA AGAATGACGT GTCGCTGGCG TCGGCTGTCT TCACCGGATC CTCCCACAGT 
TTCCCCATCT CTATCGTCCA GGTGTCCAGC ACTCCGCAGA AGGAGGAATA TCTGCTGTGT 
TTCCACGAGA TCGGAGTTTT TGTGGATGCT TACGGTCGAA GAAGTCGCCC TGATGATCTG 
AAGTGGAGTC GACTTCCCCT GACGTTCGCC TTCAGAGAGC CGTATCTCTT TGTGACCTAC 
TTCAACTCTC TGGATGTTAT TGAGATTCAG GGACACGCAG CTCTTGGGCC TCCCAGTCTG 
GTTCACCTGG ACATTCCTAA CCCACGTTAT CTGGGTCCAG CCATATCGTC TGGTGCCATC 
TATCTGGCCT CGTCCTACCA GAACAAACTG CGGGTGATCT GCTGTAAGGG CAGTCTGGTG 
CGGGAGTCTG GAGAGCTGCA GAGGACCGGC TCCAGCAGGA GCAGCCCCAA CAAGCGCGGT 
CCACCAACAT ACAACGAGCA CATCTCCAAA CGTCTGGCCT CGAGTCCTGG AGCCCAGGAG 
GGGCCCCTGC ACCGCGAGCC CAGCACTCCT CACCGCTACC GCGAGGGCCG CACCGAGTTC 
CGGAGAGACA AATCTCCAGC CCGGCCCCTG GAGCGAGAGA AATCCCCGGG CCGTCCGGTG 
CTAGACAGCC GCAGAGAGAG GTCTCCCGGT CGCTTCGAGA GCAGCGGCAG CGAGAGAGAC 
CGCGAGCGGA CACGACTGCA CACTAGCTCT GTACGCACCC AGCTGACCCC CGTCAACAAG 
GTACGCATGA GACACTGCCA CGTAAACTGT GTGTCCTTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334035.1
CDS	433..5394
Translation	MEQEISKVQRKMSDLESVLQQKDVELKASETQRNILEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSNKLQEIKEQEYHAQLEEMRVSIRQLEENLSAARRRSDLYESELKESRQTSEELKRKAVEYQQKIQKAKEQGKVEMEELLSKLQKTNGEQQVKIEELQEKLAKAVKASTEATELLQNVRQAKERMERDLERLQTKEDSSDSLRRRLRETEDGKKSLENQVKRLEMVERRESKLKDDIQTKSQQIQQMADKIMELEENLRDSQAMAQRMETQLEQKEKLFEDKIKVLETQMKADLAEKDLLESKQSTFEEESRERGKVISDQAATINAMESKMTSLEQRIAELSEANKLAANSSIYTQKNMKAQEEMISELRQQKFYLESQAGKLEAQNAKLEEHLEKLTQQEQTNKSRVLELEARLREIGLEHEEQKLEIKRQVSELTLSLQEREAQISSLQAARHALDSQLQQAKTELEETTAEAEEEITVLRAHRDEIQRKFDALRDSCAVITDLEEQLTQLTQENAELNRQNFYLSKQLDEATDENEERLQLKQDVERLRREVADREMHLNNQKQVTRQHTHTHTHTHTHRTEVADREMHLNNQKQNIETLKTTCTMLEEQVMELETLNDELMEKERQWENWRSALDEEKNQAERRARDLQRQLDSEKQNRLRAEQRNSESRQAVELAVKEHKAEILVLQQALKDQKLKAEGLSDTLGDLEKKHGMLEMNARSLQQKLETEREMKQRLMDEQAKLQQQLDMQKSHIFRLTQGLQDALDQTDLLKTERTDLEYQLENIQAAYSHEKVKMEGTITQQTKLIDFLQAKGLFGGRRREDLLAALAAQGQTQVPVVPAATPVPLQYSDLKAALDKERARCSELEDALQKTRLELREVQFNKALEHSSSPATPGLARQQLMMSTKSNKSPEHQSSGGLMSSNSSRGRKDNANPEEIRRVTSEKYTRRVKDRIHHNTPHRFTQGLNMRAARCTVCLDTVHFGRQAATCIECHALCHPKCSPYLPATCGVPTEYALHLSEALCREKGSTSGLKDMGGHMRLEGWLKQPRNGKRGQGWERKYVVLDGYKVITFETEPRDESVKPLDEFELCLSDGEVTVHSAVGSSELPNTAKTDVPYVLKLESSPQSTCWPGQTLYLMTSGYSEKQRWVAVLEAIVASSRGAREKAEADAKLLGNSLLKLEGDDRLDINCTLPLTDQIVLVGSEEGLYALNVIKNSLTHIPGLGSVFQIHIIKDHDKLLMIVGDERALCLVEIKKVKQSLSQSHLPTLPEIVPYIFETVKGCHLFASGRIENGPCICAAMPNKVTILRYNDNLNKFCIRKEIETLEPCSCIHFTSYSIIIGTNKFYEIEMKQCVLDEFLDKNDVSLASAVFTGSSHSFPISIVQVSSTPQKEEYLLCFHEIGVFVDAYGRRSRPDDLKWSRLPLTFAFREPYLFVTYFNSLDVIEIQGHAALGPPSLVHLDIPNPRYLGPAISSGAIYLASSYQNKLRVICCKGSLVRESGELQRTGSSRSSPNKRGPPTYNEHISKRLASSPGAQEGPLHREPSTPHRYREGRTEFRRDKSPARPLEREKSPGRPVLDSRRERSPGRFESSGSERDRERTRLHTSSVRTQLTPVNKVRMRHCHVNCVSF

Target ORF information:

RefSeq Version	XM_021478360.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478360.1

ORF Insert Sequence:

ATGGAGCAGG AGATTTCCAA AGTGCAGCGT AAGATGTCAG ACTTGGAGTC AGTTTTGCAG 
CAGAAAGACG TGGAGCTCAA GGCTTCAGAA ACCCAGAGAA ACATCTTAGA GCAAGACCTG 
GCAACCTATA TCACCGAATG CAGCAGTTTG AAGCGCAGTT TGGAGCAGGC CCGCATGGAG 
GTTTCGCAGG AAGATGATAA AGCTCTTCAG CTGCTGCACG ACATTCGAGA GCAGAGCAAC 
AAACTGCAGG AAATCAAAGA ACAGGAGTAC CACGCTCAGC TGGAGGAGAT GCGAGTGTCT 
ATTCGTCAGC TGGAAGAGAA TCTGTCGGCC GCTCGTCGCC GCAGTGACCT GTACGAGTCT 
GAGCTCAAAG AGTCCCGTCA GACCAGTGAG GAGCTCAAGA GGAAGGCAGT GGAGTATCAG 
CAGAAGATCC AGAAGGCTAA AGAACAAGGG AAAGTTGAGA TGGAGGAGCT TCTGAGCAAA 
CTGCAGAAGA CCAATGGAGA GCAACAAGTA AAGATTGAAG AGCTCCAGGA GAAGCTTGCT 
AAGGCAGTGA AGGCGAGTAC TGAAGCCACT GAACTCCTCC AGAATGTCCG TCAGGCTAAA 
GAGCGAATGG AGCGAGACCT GGAGCGTCTG CAGACCAAAG AGGACTCGTC CGACAGCCTG 
CGCAGACGCT TACGAGAGAC TGAGGATGGC AAAAAATCCC TGGAGAACCA GGTGAAGAGG 
CTGGAGATGG TGGAGCGGAG AGAATCCAAA CTGAAAGACG ACATTCAGAC CAAATCGCAG 
CAGATCCAGC AGATGGCCGA CAAAATCATG GAGCTTGAAG AAAATTTGCG GGACTCGCAG 
GCGATGGCGC AGCGCATGGA AACACAGCTG GAGCAGAAGG AAAAGCTTTT TGAAGACAAG 
ATCAAGGTTT TAGAAACTCA AATGAAGGCT GATTTGGCTG AGAAGGATTT GCTGGAGTCC 
AAACAGAGCA CTTTTGAAGA GGAGTCTCGC GAACGGGGAA AAGTGATCAG CGACCAGGCA 
GCGACGATCA ATGCCATGGA GTCAAAGATG ACCAGTCTGG AGCAGAGGAT CGCTGAGCTG 
TCCGAAGCCA ACAAACTGGC AGCAAACAGC AGCATTTACA CACAGAAGAA CATGAAAGCC 
CAGGAGGAGA TGATTTCTGA ACTCAGACAG CAGAAGTTTT ATTTGGAGTC TCAGGCTGGA 
AAACTGGAGG CGCAGAATGC AAAGCTGGAG GAGCATCTGG AGAAACTCAC CCAACAAGAG 
CAGACAAACA AGAGCCGAGT GCTGGAGCTG GAAGCCAGGC TGCGAGAGAT CGGCCTAGAG 
CATGAGGAAC AGAAGCTGGA GATCAAGCGT CAGGTGTCGG AGCTCACGTT GTCTCTGCAG 
GAGAGAGAAG CTCAGATCAG CAGCCTGCAA GCGGCACGCC ACGCTCTCGA CAGCCAACTA 
CAGCAGGCCA AAACTGAGCT GGAGGAGACC ACCGCCGAGG CCGAAGAGGA GATCACCGTC 
CTGAGGGCCC ATCGGGATGA AATCCAACGC AAGTTTGATG CCCTGCGAGA CAGCTGTGCG 
GTGATCACGG ATCTGGAGGA GCAGTTGACG CAGCTCACGC AAGAAAACGC GGAGCTGAAC 
CGGCAGAACT TCTACCTGTC CAAACAGCTG GACGAGGCCA CAGACGAGAA CGAGGAGCGC 
CTGCAGCTCA AGCAGGACGT GGAGCGCCTC AGGAGAGAGG TGGCAGACCG AGAGATGCAC 
CTCAACAACC AGAAACAGGT GACACGGCAA CACACACACA CACACACACA CACACACACA 
CACAGGACAG AGGTGGCAGA CCGAGAGATG CACCTCAACA ACCAGAAACA GAACATCGAG 
ACGCTGAAGA CGACATGTAC AATGCTGGAG GAGCAGGTTA TGGAGCTGGA GACTCTGAAT 
GATGAGCTGA TGGAGAAGGA GAGGCAGTGG GAGAACTGGA GATCTGCTCT GGACGAGGAG 
AAAAACCAGG CCGAGCGCAG AGCCAGAGAC CTGCAAAGAC AGCTGGACAG CGAGAAGCAG 
AACCGACTGC GTGCAGAACA GAGAAACTCT GAGTCTCGGC AGGCCGTGGA GCTGGCGGTG 
AAAGAGCACA AAGCTGAAAT CCTGGTTCTG CAGCAGGCGC TCAAAGACCA GAAACTCAAG 
GCTGAGGGCC TTTCTGACAC TCTGGGTGAT CTGGAGAAGA AGCACGGCAT GCTGGAGATG 
AACGCTCGCA GCCTGCAGCA GAAGCTGGAG ACGGAGAGAG AGATGAAGCA GAGACTCATG 
GACGAGCAAG CTAAACTGCA GCAGCAGCTG GACATGCAGA AGAGCCACAT CTTCAGACTG 
ACTCAAGGAC TGCAGGATGC GCTGGACCAG ACCGACCTGC TGAAGACCGA GCGCACAGAC 
CTGGAGTACC AGCTGGAAAA CATACAGGCG GCGTATTCTC ACGAGAAGGT GAAGATGGAG 
GGGACGATCA CCCAGCAGAC CAAGCTCATA GACTTCCTGC AGGCCAAGGG GCTGTTTGGT 
GGCCGGCGGC GGGAGGATCT GCTAGCGGCG CTGGCGGCTC AGGGACAGAC ACAGGTGCCT 
GTGGTGCCCG CCGCCACTCC TGTGCCCCTG CAGTACAGCG ACCTGAAGGC GGCGCTGGAT 
AAAGAGCGAG CCCGATGCTC CGAGCTGGAG GACGCCCTGC AGAAAACACG CCTGGAGCTC 
AGAGAAGTTC AGTTTAATAA AGCTCTGGAG CACAGCTCTT CTCCAGCCAC TCCAGGTTTG 
GCCCGTCAGC AGCTCATGAT GTCCACCAAG TCCAACAAGT CTCCAGAGCA CCAGTCCAGC 
GGCGGCCTGA TGTCCTCAAA CTCCAGCCGC GGACGCAAAG ACAACGCCAA TCCTGAAGAA 
ATAAGGAGAG TCACTTCTGA AAAGTACACC AGACGTGTGA AGGACCGGAT TCATCACAAC 
ACTCCTCACC GCTTCACGCA GGGGCTCAAT ATGAGAGCGG CTCGCTGTAC TGTGTGTCTG 
GACACAGTCC ATTTTGGACG TCAGGCTGCA ACATGCATTG AATGTCACGC GTTGTGTCAC 
CCAAAATGCT CCCCGTACCT CCCTGCCACC TGCGGCGTTC CCACTGAATA CGCGCTGCAC 
CTCTCAGAGG CTCTGTGTCG GGAGAAGGGA AGCACTTCCG GCCTGAAAGA TATGGGTGGA 
CATATGAGGC TTGAAGGATG GCTGAAGCAG CCCAGGAACG GGAAGCGCGG GCAGGGCTGG 
GAGAGGAAGT ATGTGGTGCT GGATGGATAT AAAGTGATTA CATTCGAGAC AGAACCAAGA 
GACGAGTCAG TGAAGCCGCT GGATGAGTTT GAGCTGTGTC TCTCTGATGG AGAAGTGACC 
GTTCATTCTG CTGTGGGTTC CTCTGAGCTC CCCAACACCG CTAAAACAGA TGTGCCGTAC 
GTGCTGAAGC TGGAGTCGTC TCCTCAGTCC ACATGCTGGC CCGGTCAGAC TCTGTACCTC 
ATGACCTCGG GATACTCAGA GAAGCAGCGC TGGGTGGCAG TGCTGGAAGC CATCGTGGCC 
AGCAGTCGCG GAGCCAGAGA GAAAGCTGAG GCAGATGCTA AATTATTGGG TAACTCTTTA 
CTTAAGCTAG AAGGTGACGA CCGTCTGGAT ATCAACTGCA CCCTGCCGCT GACCGACCAG 
ATTGTGTTGG TGGGCTCTGA GGAGGGTCTC TACGCACTGA ATGTGATTAA GAACTCCCTC 
ACTCATATCC CGGGTCTGGG ATCCGTGTTT CAGATCCACA TTATCAAAGA TCATGACAAG 
CTGCTGATGA TCGTGGGAGA CGAGCGAGCG CTGTGTCTGG TGGAGATTAA GAAGGTGAAG 
CAGTCTTTAT CCCAGTCTCA CCTCCCCACA CTGCCCGAGA TTGTGCCGTA TATCTTTGAG 
ACCGTCAAGG GCTGTCATCT GTTTGCCTCG GGCAGGATAG AAAATGGCCC ATGCATTTGT 
GCTGCGATGC CCAATAAAGT CACGATTCTA CGATACAATG ACAATCTTAA CAAGTTCTGC 
ATACGGAAGG AGATTGAAAC TCTGGAGCCG TGCAGCTGCA TCCACTTCAC CAGCTACAGC 
ATCATCATTG GTACCAATAA ATTCTACGAG ATCGAGATGA AGCAGTGCGT TCTGGACGAG 
TTCCTGGACA AGAATGACGT GTCGCTGGCG TCGGCTGTCT TCACCGGATC CTCCCACAGT 
TTCCCCATCT CTATCGTCCA GGTGTCCAGC ACTCCGCAGA AGGAGGAATA TCTGCTGTGT 
TTCCACGAGA TCGGAGTTTT TGTGGATGCT TACGGTCGAA GAAGTCGCCC TGATGATCTG 
AAGTGGAGTC GACTTCCCCT GACGTTCGCC TTCAGAGAGC CGTATCTCTT TGTGACCTAC 
TTCAACTCTC TGGATGTTAT TGAGATTCAG GGACACGCAG CTCTTGGGCC TCCCAGTCTG 
GTTCACCTGG ACATTCCTAA CCCACGTTAT CTGGGTCCAG CCATATCGTC TGGTGCCATC 
TATCTGGCCT CGTCCTACCA GAACAAACTG CGGGTGATCT GCTGTAAGGG CAGTCTGGTG 
CGGGAGTCTG GAGAGCTGCA GAGGACCGGC TCCAGCAGGA GCAGCCCCAA CAAGCGCGGT 
CCACCAACAT ACAACGAGCA CATCTCCAAA CGTCTGGCCT CGAGTCCTGG AGCCCAGGAG 
GGGCCCCTGC ACCGCGAGCC CAGCACTCCT CACCGCTACC GCGAGGGCCG CACCGAGTTC 
CGGAGAGACA AATCTCCAGC CCGGCCCCTG GAGCGAGAGA AATCCCCGGG CCGTCCGGTG 
CTAGACAGCC GCAGAGAGAG GTCTCCCGGT CGCTTCGAGA GCAGCGGCAG CGAGAGAGAC 
CGCGAGCGGA CACGACTGCA CACTAGCTCT GTACGCACCC AGCTGACCCC CGTCAACAAG 
GTACGCATGA GACACTGCCA CGTAAACTGT GTGTCCTTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100333880 ( XM_021478361.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334036.1 Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X9, mRNA.

Price & Availability↑

CloneID	ODa143753
Clone ID Related Accession (Same CDS sequence)	XM_021478361.1 , XM_021478362.1
Accession Version	XM_021478361.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4896bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	citron Rho-interacting kinase-like isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG AGATTTCCAA AGTGCAGCGT AAGATGTCAG ACTTGGAGTC AGTTTTGCAG 
CAGAAAGACG TGGAGCTCAA GGCTTCAGAA ACCCAGAGAA ACATCTTAGA GCAAGACCTG 
GCAACCTATA TCACCGAATG CAGCAGTTTG AAGCGCAGTT TGGAGCAGGC CCGCATGGAG 
GTTTCGCAGG AAGATGATAA AGCTCTTCAG CTGCTGCACG ACATTCGAGA GCAGAGCAAC 
AAACTGCAGG AAATCAAAGA ACAGGAGTAC CACGCTCAGC TGGAGGAGAT GCGAGTGTCT 
ATTCGTCAGC TGGAAGAGAA TCTGTCGGCC GCTCGTCGCC GCAGTGACCT GTACGAGTCT 
GAGCTCAAAG AGTCCCGTCA GACCAGTGAG GAGCTCAAGA GGAAGGCAGT GGAGTATCAG 
CAGAAGATCC AGAAGGCTAA AGAACAAGGG AAAGTTGAGA TGGAGGAGCT TCTGAGCAAA 
CTGCAGAAGA CCAATGGAGA GCAACAAGTA AAGATTGAAG AGCTCCAGGA GAAGCTTGCT 
AAGGCAGTGA AGGCGAGTAC TGAAGCCACT GAACTCCTCC AGAATGTCCG TCAGGCTAAA 
GAGCGAATGG AGCGAGACCT GGAGCGTCTG CAGACCAAAG AGGACTCGTC CGACAGCCTG 
CGCAGACGCT TACGAGAGAC TGAGGATGGC AAAAAATCCC TGGAGAACCA GGTGAAGAGG 
CTGGAGATGG TGGAGCGGAG AGAATCCAAA CTGAAAGACG ACATTCAGAC CAAATCGCAG 
CAGATCCAGC AGATGGCCGA CAAAATCATG GAGCTTGAAG AAAATTTGCG GGACTCGCAG 
GCGATGGCGC AGCGCATGGA AACACAGCTG GAGCAGAAGG AAAAGCTTTT TGAAGACAAG 
ATCAAGGTTT TAGAAACTCA AATGAAGGCT GATTTGGCTG AGAAGGATTT GCTGGAGTCC 
AAACAGAGCA CTTTTGAAGA GGAGTCTCGC GAACGGGGAA AAGTGATCAG CGACCAGGCA 
GCGACGATCA ATGCCATGGA GTCAAAGATG ACCAGTCTGG AGCAGAGGAT CGCTGAGCTG 
TCCGAAGCCA ACAAACTGGC AGCAAACAGC AGCATTTACA CACAGAAGAA CATGAAAGCC 
CAGGAGGAGA TGATTTCTGA ACTCAGACAG CAGAAGTTTT ATTTGGAGTC TCAGGCTGGA 
AAACTGGAGG CGCAGAATGC AAAGCTGGAG GAGCATCTGG AGAAACTCAC CCAACAAGAG 
CAGACAAACA AGAGCCGAGT GCTGGAGCTG GAAGCCAGGC TGCGAGAGAT CGGCCTAGAG 
CATGAGGAAC AGAAGCTGGA GATCAAGCGT CAGGTGTCGG AGCTCACGTT GTCTCTGCAG 
GAGAGAGAAG CTCAGATCAG CAGCCTGCAA GCGGCACGCC ACGCTCTCGA CAGCCAACTA 
CAGCAGGCCA AAACTGAGCT GGAGGAGACC ACCGCCGAGG CCGAAGAGGA GATCACCGTC 
CTGAGGGCCC ATCGGGATGA AATCCAACGC AAGTTTGATG CCCTGCGAGA CAGCTGTGCG 
GTGATCACGG ATCTGGAGGA GCAGTTGACG CAGCTCACGC AAGAAAACGC GGAGCTGAAC 
CGGCAGAACT TCTACCTGTC CAAACAGCTG GACGAGGCCA CAGACGAGAA CGAGGAGCGC 
CTGCAGCTCA AGCAGGACGT GGAGCGCCTC AGGAGAGAGG TGGCAGACCG AGAGATGCAC 
CTCAACAACC AGAAACAGAA CATCGAGACG CTGAAGACGA CATGTACAAT GCTGGAGGAG 
CAGGTTATGG AGCTGGAGAC TCTGAATGAT GAGCTGATGG AGAAGGAGAG GCAGTGGGAG 
AACTGGAGAT CTGCTCTGGA CGAGGAGAAA AACCAGGCCG AGCGCAGAGC CAGAGACCTG 
CAAAGACAGC TGGACAGCGA GAAGCAGAAC CGACTGCGTG CAGAACAGAG AAACTCTGAG 
TCTCGGCAGG CCGTGGAGCT GGCGGTGAAA GAGCACAAAG CTGAAATCCT GGTTCTGCAG 
CAGGCGCTCA AAGACCAGAA ACTCAAGGCT GAGGGCCTTT CTGACACTCT GGGTGATCTG 
GAGAAGAAGC ACGGCATGCT GGAGATGAAC GCTCGCAGCC TGCAGCAGAA GCTGGAGACG 
GAGAGAGAGA TGAAGCAGAG ACTCATGGAC GAGCAAGCTA AACTGCAGCA GCAGCTGGAC 
ATGCAGAAGA GCCACATCTT CAGACTGACT CAAGGACTGC AGGATGCGCT GGACCAGACC 
GACCTGCTGA AGACCGAGCG CACAGACCTG GAGTACCAGC TGGAAAACAT ACAGGCGGCG 
TATTCTCACG AGAAGGTGAA GATGGAGGGG ACGATCACCC AGCAGACCAA GCTCATAGAC 
TTCCTGCAGG CCAAGGTAGA GCACCCCTCT AAGAAGAAAA AGGGGCTGTT TGGTGGCCGG 
CGGCGGGAGG ATCTGCTAGC GGCGCTGGCG GCTCAGGGAC AGACACAGGT GCCTGTGGTG 
CCCGCCGCCA CTCCTGTGCC CCTGCAGTAC AGCGACCTGA AGGCGGCGCT GGATAAAGAG 
CGAGCCCGAT GCTCCGAGCT GGAGGACGCC CTGCAGAAAA CACGCCTGGA GCTCAGAGAA 
GTTCAGTTTA ATAAAGCTCT GGAGCACAGC TCTTCTCCAG CCACTCCAGG TTTGGCCCGT 
CAGCAGCTCA TGATGTCCAC CAAGTCCAAC AAGTCTCCAG AGCACCAGTC CAGCGGCGGC 
CTGATGTCCT CAAACTCCAG CCGCGGACGC AAAGACAACG CCAATCCTGA AGAAATAAGG 
AGAGTCACTT CTGAAAAGTA CACCAGACGT GTGAAGGACC GGATTCATCA CAACACTCCT 
CACCGCTTCA CGCAGGGGCT CAATATGAGA GCGGCTCGCT GTACTGTGTG TCTGGACACA 
GTCCATTTTG GACGTCAGGC TGCAACATGC ATTGAATGTC ACGCGTTGTG TCACCCAAAA 
TGCTCCCCGT ACCTCCCTGC CACCTGCGGC GTTCCCACTG AATACGCGCT GCACCTCTCA 
GAGGCTCTGT GTCGGGAGAA GGGAAGCACT TCCGGCCTGA AAGATATGGG TGGACATATG 
AGGCTTGAAG GATGGCTGAA GCAGCCCAGG AACGGGAAGC GCGGGCAGGG CTGGGAGAGG 
AAGTATGTGG TGCTGGATGG ATATAAAGTG ATTACATTCG AGACAGAACC AAGAGACGAG 
TCAGTGAAGC CGCTGGATGA GTTTGAGCTG TGTCTCTCTG ATGGAGAAGT GACCGTTCAT 
TCTGCTGTGG GTTCCTCTGA GCTCCCCAAC ACCGCTAAAA CAGATGTGCC GTACGTGCTG 
AAGCTGGAGT CGTCTCCTCA GTCCACATGC TGGCCCGGTC AGACTCTGTA CCTCATGACC 
TCGGGATACT CAGAGAAGCA GCGCTGGGTG GCAGTGCTGG AAGCCATCGT GGCCAGCAGT 
CGCGGAGCCA GAGAGAAAGC TGAGGCAGAT GCTAAATTAT TGGGTAACTC TTTACTTAAG 
CTAGAAGGTG ACGACCGTCT GGATATCAAC TGCACCCTGC CGCTGACCGA CCAGATTGTG 
TTGGTGGGCT CTGAGGAGGG TCTCTACGCA CTGAATGTGA TTAAGAACTC CCTCACTCAT 
ATCCCGGGTC TGGGATCCGT GTTTCAGATC CACATTATCA AAGATCATGA CAAGCTGCTG 
ATGATCGTGG GAGACGAGCG AGCGCTGTGT CTGGTGGAGA TTAAGAAGGT GAAGCAGTCT 
TTATCCCAGT CTCACCTCCC CACACTGCCC GAGATTGTGC CGTATATCTT TGAGACCGTC 
AAGGGCTGTC ATCTGTTTGC CTCGGGCAGG ATAGAAAATG GCCCATGCAT TTGTGCTGCG 
ATGCCCAATA AAGTCACGAT TCTACGATAC AATGACAATC TTAACAAGTT CTGCATACGG 
AAGGAGATTG AAACTCTGGA GCCGTGCAGC TGCATCCACT TCACCAGCTA CAGCATCATC 
ATTGGTACCA ATAAATTCTA CGAGATCGAG ATGAAGCAGT GCGTTCTGGA CGAGTTCCTG 
GACAAGAATG ACGTGTCGCT GGCGTCGGCT GTCTTCACCG GATCCTCCCA CAGTTTCCCC 
ATCTCTATCG TCCAGGTGTC CAGCACTCCG CAGAAGGAGG AATATCTGCT GTGTTTCCAC 
GAGATCGGAG TTTTTGTGGA TGCTTACGGT CGAAGAAGTC GCCCTGATGA TCTGAAGTGG 
AGTCGACTTC CCCTGACGTT CGCCTTCAGA GAGCCGTATC TCTTTGTGAC CTACTTCAAC 
TCTCTGGATG TTATTGAGAT TCAGGGACAC GCAGCTCTTG GGCCTCCCAG TCTGGTTCAC 
CTGGACATTC CTAACCCACG TTATCTGGGT CCAGCCATAT CGTCTGGTGC CATCTATCTG 
GCCTCGTCCT ACCAGAACAA ACTGCGGGTG ATCTGCTGTA AGGGCAGTCT GGTGCGGGAG 
TCTGGAGAGC TGCAGAGGAC CGGCTCCAGC AGGAGCAGCC CCAACAAGCG CGGTCCACCA 
ACATACAACG AGCACATCTC CAAACGTCTG GCCTCGAGTC CTGGAGCCCA GGAGGGGCCC 
CTGCACCGCG AGCCCAGCAC TCCTCACCGC TACCGCGAGG GCCGCACCGA GTTCCGGAGA 
GACAAATCTC CAGCCCGGCC CCTGGAGCGA GAGAAATCCC CGGGCCGTCC GGTGCTAGAC 
AGCCGCAGAG AGAGGTCTCC CGGTCGCTTC GAGAGCAGCG GCAGCGAGAG AGACCGCGAG 
CGGACACGAC TGCACACTAG CTCTGTACGC ACCCAGCTGA CCCCCGTCAA CAAGGTACGC 
ATGAGACACT GCCACGTAAA CTGTGTGTCC TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334036.1
CDS	433..5328
Translation	MEQEISKVQRKMSDLESVLQQKDVELKASETQRNILEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSNKLQEIKEQEYHAQLEEMRVSIRQLEENLSAARRRSDLYESELKESRQTSEELKRKAVEYQQKIQKAKEQGKVEMEELLSKLQKTNGEQQVKIEELQEKLAKAVKASTEATELLQNVRQAKERMERDLERLQTKEDSSDSLRRRLRETEDGKKSLENQVKRLEMVERRESKLKDDIQTKSQQIQQMADKIMELEENLRDSQAMAQRMETQLEQKEKLFEDKIKVLETQMKADLAEKDLLESKQSTFEEESRERGKVISDQAATINAMESKMTSLEQRIAELSEANKLAANSSIYTQKNMKAQEEMISELRQQKFYLESQAGKLEAQNAKLEEHLEKLTQQEQTNKSRVLELEARLREIGLEHEEQKLEIKRQVSELTLSLQEREAQISSLQAARHALDSQLQQAKTELEETTAEAEEEITVLRAHRDEIQRKFDALRDSCAVITDLEEQLTQLTQENAELNRQNFYLSKQLDEATDENEERLQLKQDVERLRREVADREMHLNNQKQNIETLKTTCTMLEEQVMELETLNDELMEKERQWENWRSALDEEKNQAERRARDLQRQLDSEKQNRLRAEQRNSESRQAVELAVKEHKAEILVLQQALKDQKLKAEGLSDTLGDLEKKHGMLEMNARSLQQKLETEREMKQRLMDEQAKLQQQLDMQKSHIFRLTQGLQDALDQTDLLKTERTDLEYQLENIQAAYSHEKVKMEGTITQQTKLIDFLQAKVEHPSKKKKGLFGGRRREDLLAALAAQGQTQVPVVPAATPVPLQYSDLKAALDKERARCSELEDALQKTRLELREVQFNKALEHSSSPATPGLARQQLMMSTKSNKSPEHQSSGGLMSSNSSRGRKDNANPEEIRRVTSEKYTRRVKDRIHHNTPHRFTQGLNMRAARCTVCLDTVHFGRQAATCIECHALCHPKCSPYLPATCGVPTEYALHLSEALCREKGSTSGLKDMGGHMRLEGWLKQPRNGKRGQGWERKYVVLDGYKVITFETEPRDESVKPLDEFELCLSDGEVTVHSAVGSSELPNTAKTDVPYVLKLESSPQSTCWPGQTLYLMTSGYSEKQRWVAVLEAIVASSRGAREKAEADAKLLGNSLLKLEGDDRLDINCTLPLTDQIVLVGSEEGLYALNVIKNSLTHIPGLGSVFQIHIIKDHDKLLMIVGDERALCLVEIKKVKQSLSQSHLPTLPEIVPYIFETVKGCHLFASGRIENGPCICAAMPNKVTILRYNDNLNKFCIRKEIETLEPCSCIHFTSYSIIIGTNKFYEIEMKQCVLDEFLDKNDVSLASAVFTGSSHSFPISIVQVSSTPQKEEYLLCFHEIGVFVDAYGRRSRPDDLKWSRLPLTFAFREPYLFVTYFNSLDVIEIQGHAALGPPSLVHLDIPNPRYLGPAISSGAIYLASSYQNKLRVICCKGSLVRESGELQRTGSSRSSPNKRGPPTYNEHISKRLASSPGAQEGPLHREPSTPHRYREGRTEFRRDKSPARPLEREKSPGRPVLDSRRERSPGRFESSGSERDRERTRLHTSSVRTQLTPVNKVRMRHCHVNCVSF

Target ORF information:

RefSeq Version	XM_021478361.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478361.1

ORF Insert Sequence:

ATGGAGCAGG AGATTTCCAA AGTGCAGCGT AAGATGTCAG ACTTGGAGTC AGTTTTGCAG 
CAGAAAGACG TGGAGCTCAA GGCTTCAGAA ACCCAGAGAA ACATCTTAGA GCAAGACCTG 
GCAACCTATA TCACCGAATG CAGCAGTTTG AAGCGCAGTT TGGAGCAGGC CCGCATGGAG 
GTTTCGCAGG AAGATGATAA AGCTCTTCAG CTGCTGCACG ACATTCGAGA GCAGAGCAAC 
AAACTGCAGG AAATCAAAGA ACAGGAGTAC CACGCTCAGC TGGAGGAGAT GCGAGTGTCT 
ATTCGTCAGC TGGAAGAGAA TCTGTCGGCC GCTCGTCGCC GCAGTGACCT GTACGAGTCT 
GAGCTCAAAG AGTCCCGTCA GACCAGTGAG GAGCTCAAGA GGAAGGCAGT GGAGTATCAG 
CAGAAGATCC AGAAGGCTAA AGAACAAGGG AAAGTTGAGA TGGAGGAGCT TCTGAGCAAA 
CTGCAGAAGA CCAATGGAGA GCAACAAGTA AAGATTGAAG AGCTCCAGGA GAAGCTTGCT 
AAGGCAGTGA AGGCGAGTAC TGAAGCCACT GAACTCCTCC AGAATGTCCG TCAGGCTAAA 
GAGCGAATGG AGCGAGACCT GGAGCGTCTG CAGACCAAAG AGGACTCGTC CGACAGCCTG 
CGCAGACGCT TACGAGAGAC TGAGGATGGC AAAAAATCCC TGGAGAACCA GGTGAAGAGG 
CTGGAGATGG TGGAGCGGAG AGAATCCAAA CTGAAAGACG ACATTCAGAC CAAATCGCAG 
CAGATCCAGC AGATGGCCGA CAAAATCATG GAGCTTGAAG AAAATTTGCG GGACTCGCAG 
GCGATGGCGC AGCGCATGGA AACACAGCTG GAGCAGAAGG AAAAGCTTTT TGAAGACAAG 
ATCAAGGTTT TAGAAACTCA AATGAAGGCT GATTTGGCTG AGAAGGATTT GCTGGAGTCC 
AAACAGAGCA CTTTTGAAGA GGAGTCTCGC GAACGGGGAA AAGTGATCAG CGACCAGGCA 
GCGACGATCA ATGCCATGGA GTCAAAGATG ACCAGTCTGG AGCAGAGGAT CGCTGAGCTG 
TCCGAAGCCA ACAAACTGGC AGCAAACAGC AGCATTTACA CACAGAAGAA CATGAAAGCC 
CAGGAGGAGA TGATTTCTGA ACTCAGACAG CAGAAGTTTT ATTTGGAGTC TCAGGCTGGA 
AAACTGGAGG CGCAGAATGC AAAGCTGGAG GAGCATCTGG AGAAACTCAC CCAACAAGAG 
CAGACAAACA AGAGCCGAGT GCTGGAGCTG GAAGCCAGGC TGCGAGAGAT CGGCCTAGAG 
CATGAGGAAC AGAAGCTGGA GATCAAGCGT CAGGTGTCGG AGCTCACGTT GTCTCTGCAG 
GAGAGAGAAG CTCAGATCAG CAGCCTGCAA GCGGCACGCC ACGCTCTCGA CAGCCAACTA 
CAGCAGGCCA AAACTGAGCT GGAGGAGACC ACCGCCGAGG CCGAAGAGGA GATCACCGTC 
CTGAGGGCCC ATCGGGATGA AATCCAACGC AAGTTTGATG CCCTGCGAGA CAGCTGTGCG 
GTGATCACGG ATCTGGAGGA GCAGTTGACG CAGCTCACGC AAGAAAACGC GGAGCTGAAC 
CGGCAGAACT TCTACCTGTC CAAACAGCTG GACGAGGCCA CAGACGAGAA CGAGGAGCGC 
CTGCAGCTCA AGCAGGACGT GGAGCGCCTC AGGAGAGAGG TGGCAGACCG AGAGATGCAC 
CTCAACAACC AGAAACAGAA CATCGAGACG CTGAAGACGA CATGTACAAT GCTGGAGGAG 
CAGGTTATGG AGCTGGAGAC TCTGAATGAT GAGCTGATGG AGAAGGAGAG GCAGTGGGAG 
AACTGGAGAT CTGCTCTGGA CGAGGAGAAA AACCAGGCCG AGCGCAGAGC CAGAGACCTG 
CAAAGACAGC TGGACAGCGA GAAGCAGAAC CGACTGCGTG CAGAACAGAG AAACTCTGAG 
TCTCGGCAGG CCGTGGAGCT GGCGGTGAAA GAGCACAAAG CTGAAATCCT GGTTCTGCAG 
CAGGCGCTCA AAGACCAGAA ACTCAAGGCT GAGGGCCTTT CTGACACTCT GGGTGATCTG 
GAGAAGAAGC ACGGCATGCT GGAGATGAAC GCTCGCAGCC TGCAGCAGAA GCTGGAGACG 
GAGAGAGAGA TGAAGCAGAG ACTCATGGAC GAGCAAGCTA AACTGCAGCA GCAGCTGGAC 
ATGCAGAAGA GCCACATCTT CAGACTGACT CAAGGACTGC AGGATGCGCT GGACCAGACC 
GACCTGCTGA AGACCGAGCG CACAGACCTG GAGTACCAGC TGGAAAACAT ACAGGCGGCG 
TATTCTCACG AGAAGGTGAA GATGGAGGGG ACGATCACCC AGCAGACCAA GCTCATAGAC 
TTCCTGCAGG CCAAGGTAGA GCACCCCTCT AAGAAGAAAA AGGGGCTGTT TGGTGGCCGG 
CGGCGGGAGG ATCTGCTAGC GGCGCTGGCG GCTCAGGGAC AGACACAGGT GCCTGTGGTG 
CCCGCCGCCA CTCCTGTGCC CCTGCAGTAC AGCGACCTGA AGGCGGCGCT GGATAAAGAG 
CGAGCCCGAT GCTCCGAGCT GGAGGACGCC CTGCAGAAAA CACGCCTGGA GCTCAGAGAA 
GTTCAGTTTA ATAAAGCTCT GGAGCACAGC TCTTCTCCAG CCACTCCAGG TTTGGCCCGT 
CAGCAGCTCA TGATGTCCAC CAAGTCCAAC AAGTCTCCAG AGCACCAGTC CAGCGGCGGC 
CTGATGTCCT CAAACTCCAG CCGCGGACGC AAAGACAACG CCAATCCTGA AGAAATAAGG 
AGAGTCACTT CTGAAAAGTA CACCAGACGT GTGAAGGACC GGATTCATCA CAACACTCCT 
CACCGCTTCA CGCAGGGGCT CAATATGAGA GCGGCTCGCT GTACTGTGTG TCTGGACACA 
GTCCATTTTG GACGTCAGGC TGCAACATGC ATTGAATGTC ACGCGTTGTG TCACCCAAAA 
TGCTCCCCGT ACCTCCCTGC CACCTGCGGC GTTCCCACTG AATACGCGCT GCACCTCTCA 
GAGGCTCTGT GTCGGGAGAA GGGAAGCACT TCCGGCCTGA AAGATATGGG TGGACATATG 
AGGCTTGAAG GATGGCTGAA GCAGCCCAGG AACGGGAAGC GCGGGCAGGG CTGGGAGAGG 
AAGTATGTGG TGCTGGATGG ATATAAAGTG ATTACATTCG AGACAGAACC AAGAGACGAG 
TCAGTGAAGC CGCTGGATGA GTTTGAGCTG TGTCTCTCTG ATGGAGAAGT GACCGTTCAT 
TCTGCTGTGG GTTCCTCTGA GCTCCCCAAC ACCGCTAAAA CAGATGTGCC GTACGTGCTG 
AAGCTGGAGT CGTCTCCTCA GTCCACATGC TGGCCCGGTC AGACTCTGTA CCTCATGACC 
TCGGGATACT CAGAGAAGCA GCGCTGGGTG GCAGTGCTGG AAGCCATCGT GGCCAGCAGT 
CGCGGAGCCA GAGAGAAAGC TGAGGCAGAT GCTAAATTAT TGGGTAACTC TTTACTTAAG 
CTAGAAGGTG ACGACCGTCT GGATATCAAC TGCACCCTGC CGCTGACCGA CCAGATTGTG 
TTGGTGGGCT CTGAGGAGGG TCTCTACGCA CTGAATGTGA TTAAGAACTC CCTCACTCAT 
ATCCCGGGTC TGGGATCCGT GTTTCAGATC CACATTATCA AAGATCATGA CAAGCTGCTG 
ATGATCGTGG GAGACGAGCG AGCGCTGTGT CTGGTGGAGA TTAAGAAGGT GAAGCAGTCT 
TTATCCCAGT CTCACCTCCC CACACTGCCC GAGATTGTGC CGTATATCTT TGAGACCGTC 
AAGGGCTGTC ATCTGTTTGC CTCGGGCAGG ATAGAAAATG GCCCATGCAT TTGTGCTGCG 
ATGCCCAATA AAGTCACGAT TCTACGATAC AATGACAATC TTAACAAGTT CTGCATACGG 
AAGGAGATTG AAACTCTGGA GCCGTGCAGC TGCATCCACT TCACCAGCTA CAGCATCATC 
ATTGGTACCA ATAAATTCTA CGAGATCGAG ATGAAGCAGT GCGTTCTGGA CGAGTTCCTG 
GACAAGAATG ACGTGTCGCT GGCGTCGGCT GTCTTCACCG GATCCTCCCA CAGTTTCCCC 
ATCTCTATCG TCCAGGTGTC CAGCACTCCG CAGAAGGAGG AATATCTGCT GTGTTTCCAC 
GAGATCGGAG TTTTTGTGGA TGCTTACGGT CGAAGAAGTC GCCCTGATGA TCTGAAGTGG 
AGTCGACTTC CCCTGACGTT CGCCTTCAGA GAGCCGTATC TCTTTGTGAC CTACTTCAAC 
TCTCTGGATG TTATTGAGAT TCAGGGACAC GCAGCTCTTG GGCCTCCCAG TCTGGTTCAC 
CTGGACATTC CTAACCCACG TTATCTGGGT CCAGCCATAT CGTCTGGTGC CATCTATCTG 
GCCTCGTCCT ACCAGAACAA ACTGCGGGTG ATCTGCTGTA AGGGCAGTCT GGTGCGGGAG 
TCTGGAGAGC TGCAGAGGAC CGGCTCCAGC AGGAGCAGCC CCAACAAGCG CGGTCCACCA 
ACATACAACG AGCACATCTC CAAACGTCTG GCCTCGAGTC CTGGAGCCCA GGAGGGGCCC 
CTGCACCGCG AGCCCAGCAC TCCTCACCGC TACCGCGAGG GCCGCACCGA GTTCCGGAGA 
GACAAATCTC CAGCCCGGCC CCTGGAGCGA GAGAAATCCC CGGGCCGTCC GGTGCTAGAC 
AGCCGCAGAG AGAGGTCTCC CGGTCGCTTC GAGAGCAGCG GCAGCGAGAG AGACCGCGAG 
CGGACACGAC TGCACACTAG CTCTGTACGC ACCCAGCTGA CCCCCGTCAA CAAGGTACGC 
ATGAGACACT GCCACGTAAA CTGTGTGTCC TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100333880 ( XM_021478362.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334037.1 Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X10, mRNA.

Price & Availability↑

CloneID	ODa143753
Clone ID Related Accession (Same CDS sequence)	XM_021478361.1 , XM_021478362.1
Accession Version	XM_021478362.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4896bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	citron Rho-interacting kinase-like isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG AGATTTCCAA AGTGCAGCGT AAGATGTCAG ACTTGGAGTC AGTTTTGCAG 
CAGAAAGACG TGGAGCTCAA GGCTTCAGAA ACCCAGAGAA ACATCTTAGA GCAAGACCTG 
GCAACCTATA TCACCGAATG CAGCAGTTTG AAGCGCAGTT TGGAGCAGGC CCGCATGGAG 
GTTTCGCAGG AAGATGATAA AGCTCTTCAG CTGCTGCACG ACATTCGAGA GCAGAGCAAC 
AAACTGCAGG AAATCAAAGA ACAGGAGTAC CACGCTCAGC TGGAGGAGAT GCGAGTGTCT 
ATTCGTCAGC TGGAAGAGAA TCTGTCGGCC GCTCGTCGCC GCAGTGACCT GTACGAGTCT 
GAGCTCAAAG AGTCCCGTCA GACCAGTGAG GAGCTCAAGA GGAAGGCAGT GGAGTATCAG 
CAGAAGATCC AGAAGGCTAA AGAACAAGGG AAAGTTGAGA TGGAGGAGCT TCTGAGCAAA 
CTGCAGAAGA CCAATGGAGA GCAACAAGTA AAGATTGAAG AGCTCCAGGA GAAGCTTGCT 
AAGGCAGTGA AGGCGAGTAC TGAAGCCACT GAACTCCTCC AGAATGTCCG TCAGGCTAAA 
GAGCGAATGG AGCGAGACCT GGAGCGTCTG CAGACCAAAG AGGACTCGTC CGACAGCCTG 
CGCAGACGCT TACGAGAGAC TGAGGATGGC AAAAAATCCC TGGAGAACCA GGTGAAGAGG 
CTGGAGATGG TGGAGCGGAG AGAATCCAAA CTGAAAGACG ACATTCAGAC CAAATCGCAG 
CAGATCCAGC AGATGGCCGA CAAAATCATG GAGCTTGAAG AAAATTTGCG GGACTCGCAG 
GCGATGGCGC AGCGCATGGA AACACAGCTG GAGCAGAAGG AAAAGCTTTT TGAAGACAAG 
ATCAAGGTTT TAGAAACTCA AATGAAGGCT GATTTGGCTG AGAAGGATTT GCTGGAGTCC 
AAACAGAGCA CTTTTGAAGA GGAGTCTCGC GAACGGGGAA AAGTGATCAG CGACCAGGCA 
GCGACGATCA ATGCCATGGA GTCAAAGATG ACCAGTCTGG AGCAGAGGAT CGCTGAGCTG 
TCCGAAGCCA ACAAACTGGC AGCAAACAGC AGCATTTACA CACAGAAGAA CATGAAAGCC 
CAGGAGGAGA TGATTTCTGA ACTCAGACAG CAGAAGTTTT ATTTGGAGTC TCAGGCTGGA 
AAACTGGAGG CGCAGAATGC AAAGCTGGAG GAGCATCTGG AGAAACTCAC CCAACAAGAG 
CAGACAAACA AGAGCCGAGT GCTGGAGCTG GAAGCCAGGC TGCGAGAGAT CGGCCTAGAG 
CATGAGGAAC AGAAGCTGGA GATCAAGCGT CAGGTGTCGG AGCTCACGTT GTCTCTGCAG 
GAGAGAGAAG CTCAGATCAG CAGCCTGCAA GCGGCACGCC ACGCTCTCGA CAGCCAACTA 
CAGCAGGCCA AAACTGAGCT GGAGGAGACC ACCGCCGAGG CCGAAGAGGA GATCACCGTC 
CTGAGGGCCC ATCGGGATGA AATCCAACGC AAGTTTGATG CCCTGCGAGA CAGCTGTGCG 
GTGATCACGG ATCTGGAGGA GCAGTTGACG CAGCTCACGC AAGAAAACGC GGAGCTGAAC 
CGGCAGAACT TCTACCTGTC CAAACAGCTG GACGAGGCCA CAGACGAGAA CGAGGAGCGC 
CTGCAGCTCA AGCAGGACGT GGAGCGCCTC AGGAGAGAGG TGGCAGACCG AGAGATGCAC 
CTCAACAACC AGAAACAGAA CATCGAGACG CTGAAGACGA CATGTACAAT GCTGGAGGAG 
CAGGTTATGG AGCTGGAGAC TCTGAATGAT GAGCTGATGG AGAAGGAGAG GCAGTGGGAG 
AACTGGAGAT CTGCTCTGGA CGAGGAGAAA AACCAGGCCG AGCGCAGAGC CAGAGACCTG 
CAAAGACAGC TGGACAGCGA GAAGCAGAAC CGACTGCGTG CAGAACAGAG AAACTCTGAG 
TCTCGGCAGG CCGTGGAGCT GGCGGTGAAA GAGCACAAAG CTGAAATCCT GGTTCTGCAG 
CAGGCGCTCA AAGACCAGAA ACTCAAGGCT GAGGGCCTTT CTGACACTCT GGGTGATCTG 
GAGAAGAAGC ACGGCATGCT GGAGATGAAC GCTCGCAGCC TGCAGCAGAA GCTGGAGACG 
GAGAGAGAGA TGAAGCAGAG ACTCATGGAC GAGCAAGCTA AACTGCAGCA GCAGCTGGAC 
ATGCAGAAGA GCCACATCTT CAGACTGACT CAAGGACTGC AGGATGCGCT GGACCAGACC 
GACCTGCTGA AGACCGAGCG CACAGACCTG GAGTACCAGC TGGAAAACAT ACAGGCGGCG 
TATTCTCACG AGAAGGTGAA GATGGAGGGG ACGATCACCC AGCAGACCAA GCTCATAGAC 
TTCCTGCAGG CCAAGGTAGA GCACCCCTCT AAGAAGAAAA AGGGGCTGTT TGGTGGCCGG 
CGGCGGGAGG ATCTGCTAGC GGCGCTGGCG GCTCAGGGAC AGACACAGGT GCCTGTGGTG 
CCCGCCGCCA CTCCTGTGCC CCTGCAGTAC AGCGACCTGA AGGCGGCGCT GGATAAAGAG 
CGAGCCCGAT GCTCCGAGCT GGAGGACGCC CTGCAGAAAA CACGCCTGGA GCTCAGAGAA 
GTTCAGTTTA ATAAAGCTCT GGAGCACAGC TCTTCTCCAG CCACTCCAGG TTTGGCCCGT 
CAGCAGCTCA TGATGTCCAC CAAGTCCAAC AAGTCTCCAG AGCACCAGTC CAGCGGCGGC 
CTGATGTCCT CAAACTCCAG CCGCGGACGC AAAGACAACG CCAATCCTGA AGAAATAAGG 
AGAGTCACTT CTGAAAAGTA CACCAGACGT GTGAAGGACC GGATTCATCA CAACACTCCT 
CACCGCTTCA CGCAGGGGCT CAATATGAGA GCGGCTCGCT GTACTGTGTG TCTGGACACA 
GTCCATTTTG GACGTCAGGC TGCAACATGC ATTGAATGTC ACGCGTTGTG TCACCCAAAA 
TGCTCCCCGT ACCTCCCTGC CACCTGCGGC GTTCCCACTG AATACGCGCT GCACCTCTCA 
GAGGCTCTGT GTCGGGAGAA GGGAAGCACT TCCGGCCTGA AAGATATGGG TGGACATATG 
AGGCTTGAAG GATGGCTGAA GCAGCCCAGG AACGGGAAGC GCGGGCAGGG CTGGGAGAGG 
AAGTATGTGG TGCTGGATGG ATATAAAGTG ATTACATTCG AGACAGAACC AAGAGACGAG 
TCAGTGAAGC CGCTGGATGA GTTTGAGCTG TGTCTCTCTG ATGGAGAAGT GACCGTTCAT 
TCTGCTGTGG GTTCCTCTGA GCTCCCCAAC ACCGCTAAAA CAGATGTGCC GTACGTGCTG 
AAGCTGGAGT CGTCTCCTCA GTCCACATGC TGGCCCGGTC AGACTCTGTA CCTCATGACC 
TCGGGATACT CAGAGAAGCA GCGCTGGGTG GCAGTGCTGG AAGCCATCGT GGCCAGCAGT 
CGCGGAGCCA GAGAGAAAGC TGAGGCAGAT GCTAAATTAT TGGGTAACTC TTTACTTAAG 
CTAGAAGGTG ACGACCGTCT GGATATCAAC TGCACCCTGC CGCTGACCGA CCAGATTGTG 
TTGGTGGGCT CTGAGGAGGG TCTCTACGCA CTGAATGTGA TTAAGAACTC CCTCACTCAT 
ATCCCGGGTC TGGGATCCGT GTTTCAGATC CACATTATCA AAGATCATGA CAAGCTGCTG 
ATGATCGTGG GAGACGAGCG AGCGCTGTGT CTGGTGGAGA TTAAGAAGGT GAAGCAGTCT 
TTATCCCAGT CTCACCTCCC CACACTGCCC GAGATTGTGC CGTATATCTT TGAGACCGTC 
AAGGGCTGTC ATCTGTTTGC CTCGGGCAGG ATAGAAAATG GCCCATGCAT TTGTGCTGCG 
ATGCCCAATA AAGTCACGAT TCTACGATAC AATGACAATC TTAACAAGTT CTGCATACGG 
AAGGAGATTG AAACTCTGGA GCCGTGCAGC TGCATCCACT TCACCAGCTA CAGCATCATC 
ATTGGTACCA ATAAATTCTA CGAGATCGAG ATGAAGCAGT GCGTTCTGGA CGAGTTCCTG 
GACAAGAATG ACGTGTCGCT GGCGTCGGCT GTCTTCACCG GATCCTCCCA CAGTTTCCCC 
ATCTCTATCG TCCAGGTGTC CAGCACTCCG CAGAAGGAGG AATATCTGCT GTGTTTCCAC 
GAGATCGGAG TTTTTGTGGA TGCTTACGGT CGAAGAAGTC GCCCTGATGA TCTGAAGTGG 
AGTCGACTTC CCCTGACGTT CGCCTTCAGA GAGCCGTATC TCTTTGTGAC CTACTTCAAC 
TCTCTGGATG TTATTGAGAT TCAGGGACAC GCAGCTCTTG GGCCTCCCAG TCTGGTTCAC 
CTGGACATTC CTAACCCACG TTATCTGGGT CCAGCCATAT CGTCTGGTGC CATCTATCTG 
GCCTCGTCCT ACCAGAACAA ACTGCGGGTG ATCTGCTGTA AGGGCAGTCT GGTGCGGGAG 
TCTGGAGAGC TGCAGAGGAC CGGCTCCAGC AGGAGCAGCC CCAACAAGCG CGGTCCACCA 
ACATACAACG AGCACATCTC CAAACGTCTG GCCTCGAGTC CTGGAGCCCA GGAGGGGCCC 
CTGCACCGCG AGCCCAGCAC TCCTCACCGC TACCGCGAGG GCCGCACCGA GTTCCGGAGA 
GACAAATCTC CAGCCCGGCC CCTGGAGCGA GAGAAATCCC CGGGCCGTCC GGTGCTAGAC 
AGCCGCAGAG AGAGGTCTCC CGGTCGCTTC GAGAGCAGCG GCAGCGAGAG AGACCGCGAG 
CGGACACGAC TGCACACTAG CTCTGTACGC ACCCAGCTGA CCCCCGTCAA CAAGGTACGC 
ATGAGACACT GCCACGTAAA CTGTGTGTCC TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334037.1
CDS	433..5328
Translation	MEQEISKVQRKMSDLESVLQQKDVELKASETQRNILEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSNKLQEIKEQEYHAQLEEMRVSIRQLEENLSAARRRSDLYESELKESRQTSEELKRKAVEYQQKIQKAKEQGKVEMEELLSKLQKTNGEQQVKIEELQEKLAKAVKASTEATELLQNVRQAKERMERDLERLQTKEDSSDSLRRRLRETEDGKKSLENQVKRLEMVERRESKLKDDIQTKSQQIQQMADKIMELEENLRDSQAMAQRMETQLEQKEKLFEDKIKVLETQMKADLAEKDLLESKQSTFEEESRERGKVISDQAATINAMESKMTSLEQRIAELSEANKLAANSSIYTQKNMKAQEEMISELRQQKFYLESQAGKLEAQNAKLEEHLEKLTQQEQTNKSRVLELEARLREIGLEHEEQKLEIKRQVSELTLSLQEREAQISSLQAARHALDSQLQQAKTELEETTAEAEEEITVLRAHRDEIQRKFDALRDSCAVITDLEEQLTQLTQENAELNRQNFYLSKQLDEATDENEERLQLKQDVERLRREVADREMHLNNQKQNIETLKTTCTMLEEQVMELETLNDELMEKERQWENWRSALDEEKNQAERRARDLQRQLDSEKQNRLRAEQRNSESRQAVELAVKEHKAEILVLQQALKDQKLKAEGLSDTLGDLEKKHGMLEMNARSLQQKLETEREMKQRLMDEQAKLQQQLDMQKSHIFRLTQGLQDALDQTDLLKTERTDLEYQLENIQAAYSHEKVKMEGTITQQTKLIDFLQAKVEHPSKKKKGLFGGRRREDLLAALAAQGQTQVPVVPAATPVPLQYSDLKAALDKERARCSELEDALQKTRLELREVQFNKALEHSSSPATPGLARQQLMMSTKSNKSPEHQSSGGLMSSNSSRGRKDNANPEEIRRVTSEKYTRRVKDRIHHNTPHRFTQGLNMRAARCTVCLDTVHFGRQAATCIECHALCHPKCSPYLPATCGVPTEYALHLSEALCREKGSTSGLKDMGGHMRLEGWLKQPRNGKRGQGWERKYVVLDGYKVITFETEPRDESVKPLDEFELCLSDGEVTVHSAVGSSELPNTAKTDVPYVLKLESSPQSTCWPGQTLYLMTSGYSEKQRWVAVLEAIVASSRGAREKAEADAKLLGNSLLKLEGDDRLDINCTLPLTDQIVLVGSEEGLYALNVIKNSLTHIPGLGSVFQIHIIKDHDKLLMIVGDERALCLVEIKKVKQSLSQSHLPTLPEIVPYIFETVKGCHLFASGRIENGPCICAAMPNKVTILRYNDNLNKFCIRKEIETLEPCSCIHFTSYSIIIGTNKFYEIEMKQCVLDEFLDKNDVSLASAVFTGSSHSFPISIVQVSSTPQKEEYLLCFHEIGVFVDAYGRRSRPDDLKWSRLPLTFAFREPYLFVTYFNSLDVIEIQGHAALGPPSLVHLDIPNPRYLGPAISSGAIYLASSYQNKLRVICCKGSLVRESGELQRTGSSRSSPNKRGPPTYNEHISKRLASSPGAQEGPLHREPSTPHRYREGRTEFRRDKSPARPLEREKSPGRPVLDSRRERSPGRFESSGSERDRERTRLHTSSVRTQLTPVNKVRMRHCHVNCVSF

Target ORF information:

RefSeq Version	XM_021478362.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478362.1

ORF Insert Sequence:

ATGGAGCAGG AGATTTCCAA AGTGCAGCGT AAGATGTCAG ACTTGGAGTC AGTTTTGCAG 
CAGAAAGACG TGGAGCTCAA GGCTTCAGAA ACCCAGAGAA ACATCTTAGA GCAAGACCTG 
GCAACCTATA TCACCGAATG CAGCAGTTTG AAGCGCAGTT TGGAGCAGGC CCGCATGGAG 
GTTTCGCAGG AAGATGATAA AGCTCTTCAG CTGCTGCACG ACATTCGAGA GCAGAGCAAC 
AAACTGCAGG AAATCAAAGA ACAGGAGTAC CACGCTCAGC TGGAGGAGAT GCGAGTGTCT 
ATTCGTCAGC TGGAAGAGAA TCTGTCGGCC GCTCGTCGCC GCAGTGACCT GTACGAGTCT 
GAGCTCAAAG AGTCCCGTCA GACCAGTGAG GAGCTCAAGA GGAAGGCAGT GGAGTATCAG 
CAGAAGATCC AGAAGGCTAA AGAACAAGGG AAAGTTGAGA TGGAGGAGCT TCTGAGCAAA 
CTGCAGAAGA CCAATGGAGA GCAACAAGTA AAGATTGAAG AGCTCCAGGA GAAGCTTGCT 
AAGGCAGTGA AGGCGAGTAC TGAAGCCACT GAACTCCTCC AGAATGTCCG TCAGGCTAAA 
GAGCGAATGG AGCGAGACCT GGAGCGTCTG CAGACCAAAG AGGACTCGTC CGACAGCCTG 
CGCAGACGCT TACGAGAGAC TGAGGATGGC AAAAAATCCC TGGAGAACCA GGTGAAGAGG 
CTGGAGATGG TGGAGCGGAG AGAATCCAAA CTGAAAGACG ACATTCAGAC CAAATCGCAG 
CAGATCCAGC AGATGGCCGA CAAAATCATG GAGCTTGAAG AAAATTTGCG GGACTCGCAG 
GCGATGGCGC AGCGCATGGA AACACAGCTG GAGCAGAAGG AAAAGCTTTT TGAAGACAAG 
ATCAAGGTTT TAGAAACTCA AATGAAGGCT GATTTGGCTG AGAAGGATTT GCTGGAGTCC 
AAACAGAGCA CTTTTGAAGA GGAGTCTCGC GAACGGGGAA AAGTGATCAG CGACCAGGCA 
GCGACGATCA ATGCCATGGA GTCAAAGATG ACCAGTCTGG AGCAGAGGAT CGCTGAGCTG 
TCCGAAGCCA ACAAACTGGC AGCAAACAGC AGCATTTACA CACAGAAGAA CATGAAAGCC 
CAGGAGGAGA TGATTTCTGA ACTCAGACAG CAGAAGTTTT ATTTGGAGTC TCAGGCTGGA 
AAACTGGAGG CGCAGAATGC AAAGCTGGAG GAGCATCTGG AGAAACTCAC CCAACAAGAG 
CAGACAAACA AGAGCCGAGT GCTGGAGCTG GAAGCCAGGC TGCGAGAGAT CGGCCTAGAG 
CATGAGGAAC AGAAGCTGGA GATCAAGCGT CAGGTGTCGG AGCTCACGTT GTCTCTGCAG 
GAGAGAGAAG CTCAGATCAG CAGCCTGCAA GCGGCACGCC ACGCTCTCGA CAGCCAACTA 
CAGCAGGCCA AAACTGAGCT GGAGGAGACC ACCGCCGAGG CCGAAGAGGA GATCACCGTC 
CTGAGGGCCC ATCGGGATGA AATCCAACGC AAGTTTGATG CCCTGCGAGA CAGCTGTGCG 
GTGATCACGG ATCTGGAGGA GCAGTTGACG CAGCTCACGC AAGAAAACGC GGAGCTGAAC 
CGGCAGAACT TCTACCTGTC CAAACAGCTG GACGAGGCCA CAGACGAGAA CGAGGAGCGC 
CTGCAGCTCA AGCAGGACGT GGAGCGCCTC AGGAGAGAGG TGGCAGACCG AGAGATGCAC 
CTCAACAACC AGAAACAGAA CATCGAGACG CTGAAGACGA CATGTACAAT GCTGGAGGAG 
CAGGTTATGG AGCTGGAGAC TCTGAATGAT GAGCTGATGG AGAAGGAGAG GCAGTGGGAG 
AACTGGAGAT CTGCTCTGGA CGAGGAGAAA AACCAGGCCG AGCGCAGAGC CAGAGACCTG 
CAAAGACAGC TGGACAGCGA GAAGCAGAAC CGACTGCGTG CAGAACAGAG AAACTCTGAG 
TCTCGGCAGG CCGTGGAGCT GGCGGTGAAA GAGCACAAAG CTGAAATCCT GGTTCTGCAG 
CAGGCGCTCA AAGACCAGAA ACTCAAGGCT GAGGGCCTTT CTGACACTCT GGGTGATCTG 
GAGAAGAAGC ACGGCATGCT GGAGATGAAC GCTCGCAGCC TGCAGCAGAA GCTGGAGACG 
GAGAGAGAGA TGAAGCAGAG ACTCATGGAC GAGCAAGCTA AACTGCAGCA GCAGCTGGAC 
ATGCAGAAGA GCCACATCTT CAGACTGACT CAAGGACTGC AGGATGCGCT GGACCAGACC 
GACCTGCTGA AGACCGAGCG CACAGACCTG GAGTACCAGC TGGAAAACAT ACAGGCGGCG 
TATTCTCACG AGAAGGTGAA GATGGAGGGG ACGATCACCC AGCAGACCAA GCTCATAGAC 
TTCCTGCAGG CCAAGGTAGA GCACCCCTCT AAGAAGAAAA AGGGGCTGTT TGGTGGCCGG 
CGGCGGGAGG ATCTGCTAGC GGCGCTGGCG GCTCAGGGAC AGACACAGGT GCCTGTGGTG 
CCCGCCGCCA CTCCTGTGCC CCTGCAGTAC AGCGACCTGA AGGCGGCGCT GGATAAAGAG 
CGAGCCCGAT GCTCCGAGCT GGAGGACGCC CTGCAGAAAA CACGCCTGGA GCTCAGAGAA 
GTTCAGTTTA ATAAAGCTCT GGAGCACAGC TCTTCTCCAG CCACTCCAGG TTTGGCCCGT 
CAGCAGCTCA TGATGTCCAC CAAGTCCAAC AAGTCTCCAG AGCACCAGTC CAGCGGCGGC 
CTGATGTCCT CAAACTCCAG CCGCGGACGC AAAGACAACG CCAATCCTGA AGAAATAAGG 
AGAGTCACTT CTGAAAAGTA CACCAGACGT GTGAAGGACC GGATTCATCA CAACACTCCT 
CACCGCTTCA CGCAGGGGCT CAATATGAGA GCGGCTCGCT GTACTGTGTG TCTGGACACA 
GTCCATTTTG GACGTCAGGC TGCAACATGC ATTGAATGTC ACGCGTTGTG TCACCCAAAA 
TGCTCCCCGT ACCTCCCTGC CACCTGCGGC GTTCCCACTG AATACGCGCT GCACCTCTCA 
GAGGCTCTGT GTCGGGAGAA GGGAAGCACT TCCGGCCTGA AAGATATGGG TGGACATATG 
AGGCTTGAAG GATGGCTGAA GCAGCCCAGG AACGGGAAGC GCGGGCAGGG CTGGGAGAGG 
AAGTATGTGG TGCTGGATGG ATATAAAGTG ATTACATTCG AGACAGAACC AAGAGACGAG 
TCAGTGAAGC CGCTGGATGA GTTTGAGCTG TGTCTCTCTG ATGGAGAAGT GACCGTTCAT 
TCTGCTGTGG GTTCCTCTGA GCTCCCCAAC ACCGCTAAAA CAGATGTGCC GTACGTGCTG 
AAGCTGGAGT CGTCTCCTCA GTCCACATGC TGGCCCGGTC AGACTCTGTA CCTCATGACC 
TCGGGATACT CAGAGAAGCA GCGCTGGGTG GCAGTGCTGG AAGCCATCGT GGCCAGCAGT 
CGCGGAGCCA GAGAGAAAGC TGAGGCAGAT GCTAAATTAT TGGGTAACTC TTTACTTAAG 
CTAGAAGGTG ACGACCGTCT GGATATCAAC TGCACCCTGC CGCTGACCGA CCAGATTGTG 
TTGGTGGGCT CTGAGGAGGG TCTCTACGCA CTGAATGTGA TTAAGAACTC CCTCACTCAT 
ATCCCGGGTC TGGGATCCGT GTTTCAGATC CACATTATCA AAGATCATGA CAAGCTGCTG 
ATGATCGTGG GAGACGAGCG AGCGCTGTGT CTGGTGGAGA TTAAGAAGGT GAAGCAGTCT 
TTATCCCAGT CTCACCTCCC CACACTGCCC GAGATTGTGC CGTATATCTT TGAGACCGTC 
AAGGGCTGTC ATCTGTTTGC CTCGGGCAGG ATAGAAAATG GCCCATGCAT TTGTGCTGCG 
ATGCCCAATA AAGTCACGAT TCTACGATAC AATGACAATC TTAACAAGTT CTGCATACGG 
AAGGAGATTG AAACTCTGGA GCCGTGCAGC TGCATCCACT TCACCAGCTA CAGCATCATC 
ATTGGTACCA ATAAATTCTA CGAGATCGAG ATGAAGCAGT GCGTTCTGGA CGAGTTCCTG 
GACAAGAATG ACGTGTCGCT GGCGTCGGCT GTCTTCACCG GATCCTCCCA CAGTTTCCCC 
ATCTCTATCG TCCAGGTGTC CAGCACTCCG CAGAAGGAGG AATATCTGCT GTGTTTCCAC 
GAGATCGGAG TTTTTGTGGA TGCTTACGGT CGAAGAAGTC GCCCTGATGA TCTGAAGTGG 
AGTCGACTTC CCCTGACGTT CGCCTTCAGA GAGCCGTATC TCTTTGTGAC CTACTTCAAC 
TCTCTGGATG TTATTGAGAT TCAGGGACAC GCAGCTCTTG GGCCTCCCAG TCTGGTTCAC 
CTGGACATTC CTAACCCACG TTATCTGGGT CCAGCCATAT CGTCTGGTGC CATCTATCTG 
GCCTCGTCCT ACCAGAACAA ACTGCGGGTG ATCTGCTGTA AGGGCAGTCT GGTGCGGGAG 
TCTGGAGAGC TGCAGAGGAC CGGCTCCAGC AGGAGCAGCC CCAACAAGCG CGGTCCACCA 
ACATACAACG AGCACATCTC CAAACGTCTG GCCTCGAGTC CTGGAGCCCA GGAGGGGCCC 
CTGCACCGCG AGCCCAGCAC TCCTCACCGC TACCGCGAGG GCCGCACCGA GTTCCGGAGA 
GACAAATCTC CAGCCCGGCC CCTGGAGCGA GAGAAATCCC CGGGCCGTCC GGTGCTAGAC 
AGCCGCAGAG AGAGGTCTCC CGGTCGCTTC GAGAGCAGCG GCAGCGAGAG AGACCGCGAG 
CGGACACGAC TGCACACTAG CTCTGTACGC ACCCAGCTGA CCCCCGTCAA CAAGGTACGC 
ATGAGACACT GCCACGTAAA CTGTGTGTCC TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100333880 ( XM_017357401.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212890.1 Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X2, mRNA.

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CloneID	ODa60005
Clone ID Related Accession (Same CDS sequence)	XM_017357401.1 , XM_017357400.1 , XM_017357400.2
Accession Version	XM_017357401.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	citron Rho-interacting kinase-like isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG AGATTTCCAA AGTGCAGCGT AAGATGTCAG ACTTGGAGTC AGTTTTGCAG 
CAGAAAGACG TGGAGCTCAA GGCTTCAGAA ACCCAGAGAA ACATCTTAGA GCAAGACCTG 
GCAACCTATA TCACCGAATG CAGCAGTTTG AAGCGCAGTT TGGAGCAGGC CCGCATGGAG 
GTTTCGCAGG AAGATGATAA AGCTCTTCAG CTGCTGCACG ACATTCGAGA GCAGAGCAAC 
AAACTGCAGG AAATCAAAGA ACAGGAGTAC CACGCTCAGC TGGAGGAGAT GCGAGTGTCT 
ATTCGTCAGC TGGAAGAGAA TCTGTCGGCC GCTCGTCGCC GCAGTGACCT GTACGAGTCT 
GAGCTCAAAG AGTCCCGTCA GACCAGTGAG GAGCTCAAGA GGAAGGCAGT GGAGTATCAG 
CAGAAGATCC AGAAGGCTAA AGAACAAGGG AAAGTTGAGA TGGAGGAGCT TCTGAGCAAA 
CTGCAGAAGA CCAATGGAGA GCAACAAGTA AAGATTGAAG AGCTCCAGGA GAAGCTTGCT 
AAGGCAGTGA AGGCGAGTAC TGAAGCCACT GAACTCCTCC AGAATGTCCG TCAGGCTAAA 
GAGCGAATGG AGCGAGACCT GGAGCGTCTG CAGACCAAAG AGGACTCGTC CGACAGCCTG 
CGCAGACGCT TACGAGAGAC TGAGGATGGC AAAAAATCCC TGGAGAACCA GGTGAAGAGG 
CTGGAGATGG TGGAGCGGAG AGAATCCAAA CTGAAAGACG ACATTCAGAC CAAATCGCAG 
CAGATCCAGC AGATGGCCGA CAAAATCATG GAGCTTGAAG AAAATTTGCG GGACTCGCAG 
GCGATGGCGC AGCGCATGGA AACACAGCTG GAGCAGAAGG AAAAGCTTTT TGAAGACAAG 
ATCAAGGTTT TAGAAACTCA AATGAAGGCT GATTTGGCTG AGAAGGATTT GCTGGAGTCC 
AAACAGAGCA CTTTTGAAGA GGAGTCTCGC GAACGGGGAA AAGTGATCAG CGACCAGGCA 
GCGACGATCA ATGCCATGGA GTCAAAGATG ACCAGTCTGG AGCAGAGGAT CGCTGAGCTG 
TCCGAAGCCA ACAAACTGGC AGCAAACAGC AGCATTTACA CACAGAAGAA CATGAAAGCC 
CAGGAGGAGA TGATTTCTGA ACTCAGACAG CAGAAGTTTT ATTTGGAGTC TCAGGCTGGA 
AAACTGGAGG CGCAGAATGC AAAGCTGGAG GAGCATCTGG AGAAACTCAC CCAACAAGAG 
CAGACAAACA AGAGCCGAGT GCTGGAGCTG GAAGCCAGGC TGCGAGAGAT CGGCCTAGAG 
CATGAGGAAC AGAAGCTGGA GATCAAGCGT CAGGTGTCGG AGCTCACGTT GTCTCTGCAG 
GAGAGAGAAG CTCAGATCAG CAGCCTGCAA GCGGCACGCC ACGCTCTCGA CAGCCAACTA 
CAGCAGGCCA AAACTGAGCT GGAGGAGACC ACCGCCGAGG CCGAAGAGGA GATCACCGTC 
CTGAGGGCCC ATCGGGATGA AATCCAACGC AAGTTTGATG CCCTGCGAGA CAGCTGTGCG 
GTGATCACGG ATCTGGAGGA GCAGTTGACG CAGCTCACGC AAGAAAACGC GGAGCTGAAC 
CGGCAGAACT TCTACCTGTC CAAACAGCTG GACGAGGCCA CAGACGAGAA CGAGGAGCGC 
CTGCAGCTCA AGCAGGACGT GGAGCGCCTC AGGAGAGAGG TGGCAGACCG AGAGATGCAC 
CTCAACAACC AGAAACAGGT GACACGGCAA CACACACACA CACACACACA CACACACACA 
CACAGGACAG AGGTGGCAGA CCGAGAGATG CACCTCAACA ACCAGAAACA GAACATCGAG 
ACGCTGAAGA CGACATGTAC AATGCTGGAG GAGCAGGTTA TGGAGCTGGA GACTCTGAAT 
GATGAGCTGA TGGAGAAGGA GAGGCAGTGG GAGAACTGGA GATCTGCTCT GGACGAGGAG 
AAAAACCAGG CCGAGCGCAG AGCCAGAGAC CTGCAAAGAC AGCTGGACAG CGAGAAGCAG 
AACCGACTGC GTGCAGAACA GAGAAACTCT GAGTCTCGGC AGGCCGTGGA GCTGGCGGTG 
AAAGAGCACA AAGCTGAAAT CCTGGTTCTG CAGCAGGCGC TCAAAGACCA GAAACTCAAG 
GCTGAGGGCC TTTCTGACAC TCTGGGTGAT CTGGAGAAGA AGCACGGCAT GCTGGAGATG 
AACGCTCGCA GCCTGCAGCA GAAGCTGGAG ACGGAGAGAG AGATGAAGCA GAGACTCATG 
GACGAGCAAG CTAAACTGCA GCAGCAGCTG GACATGCAGA AGAGCCACAT CTTCAGACTG 
ACTCAAGGAC TGCAGGATGC GCTGGACCAG ACCGACCTGC TGAAGACCGA GCGCACAGAC 
CTGGAGTACC AGCTGGAAAA CATACAGGCG GCGTATTCTC ACGAGAAGGT GAAGATGGAG 
GGGACGATCA CCCAGCAGAC CAAGCTCATA GACTTCCTGC AGGCCAAGGT AGAGCACCCC 
TCTAAGAAGA AAAAGGGGCT GTTTGGTGGC CGGCGGCGGG AGGATCTGCT AGCGGCGCTG 
GCGGCTCAGG GACAGACACA GGTGCCTGTG GTGCCCGCCG CCACTCCTGT GCCCCTGCAG 
TACAGCGACC TGAAGGCGGC GCTGGATAAA GAGCGAGCCC GATGCTCCGA GCTGGAGGAC 
GCCCTGCAGA AAACACGCCT GGAGCTCAGA GAAGTTCAGT TTAATAAAGC TCTGGAGCAC 
AGCTCTTCTC CAGCCACTCC AGGTTTGGCC CGTCAGCAGC TCATGATGTC CACCAAGTCC 
AACAAGTCTC CAGAGCACCA GTCCAGCGGC GGCCTGATGT CCTCAAACTC CAGCCGCGGA 
CGCAAAGACA ACGCCAATCC TGAAGAAATA AGGAGAGTCA CTTCTGAAAA GTACACCAGA 
CGTGTGAAGG ACCGGATTCA TCACAACACT CCTCACCGCT TCACGCAGGG GCTCAATATG 
AGAGCGGCTC GCTGTACTGT GTGTCTGGAC ACAGTCCATT TTGGACGTCA GGCTGCAACA 
TGCATTGAAT GTCACGCGTT GTGTCACCCA AAATGCTCCC CGTACCTCCC TGCCACCTGC 
GGCGTTCCCA CTGAATACGC GCTGCACCTC TCAGAGGCTC TGTGTCGGGA GAAGGGAAGC 
ACTTCCGGCC TGAAAGATAT GGGTGGACAT ATGAGGCTTG AAGGATGGCT GAAGCAGCCC 
AGGAACGGGA AGCGCGGGCA GGGCTGGGAG AGGAAGTATG TGGTGCTGGA TGGATATAAA 
GTGATTACAT TCGAGACAGA ACCAAGAGAC GAGTCAGTGA AGCCGCTGGA TGAGTTTGAG 
CTGTGTCTCT CTGATGGAGA AGTGACCGTT CATTCTGCTG TGGGTTCCTC TGAGCTCCCC 
AACACCGCTA AAACAGATGT GCCGTACGTG CTGAAGCTGG AGTCGTCTCC TCAGTCCACA 
TGCTGGCCCG GTCAGACTCT GTACCTCATG ACCTCGGGAT ACTCAGAGAA GCAGCGCTGG 
GTGGCAGTGC TGGAAGCCAT CGTGGCCAGC AGTCGCGGAG CCAGAGAGAA AGCTGAGGCA 
GATGCTAAAT TATTGGGTAA CTCTTTACTT AAGCTAGAAG GTGACGACCG TCTGGATATC 
AACTGCACCC TGCCGCTGAC CGACCAGATT GTGTTGGTGG GCTCTGAGGA GGGTCTCTAC 
GCACTGAATG TGATTAAGAA CTCCCTCACT CATATCCCGG GTCTGGGATC CGTGTTTCAG 
ATCCACATTA TCAAAGATCA TGACAAGCTG CTGATGATCG TGGGAGACGA GCGAGCGCTG 
TGTCTGGTGG AGATTAAGAA GGTGAAGCAG TCTTTATCCC AGTCTCACCT CCCCACACTG 
CCCGAGATTG TGCCGTATAT CTTTGAGACC GTCAAGGGCT GTCATCTGTT TGCCTCGGGC 
AGGATAGAAA ATGGCCCATG CATTTGTGCT GCGATGCCCA ATAAAGTCAC GATTCTACGA 
TACAATGACA ATCTTAACAA GTTCTGCATA CGGAAGGAGA TTGAAACTCT GGAGCCGTGC 
AGCTGCATCC ACTTCACCAG CTACAGCATC ATCATTGGTA CCAATAAATT CTACGAGATC 
GAGATGAAGC AGTGCGTTCT GGACGAGTTC CTGGACAAGA ATGACGTGTC GCTGGCGTCG 
GCTGTCTTCA CCGGATCCTC CCACAGTTTC CCCATCTCTA TCGTCCAGGT GTCCAGCACT 
CCGCAGAAGG AGGAATATCT GCTGTGTTTC CACGAGATCG GAGTTTTTGT GGATGCTTAC 
GGTCGAAGAA GTCGCCCTGA TGATCTGAAG TGGAGTCGAC TTCCCCTGAC GTTCGCCTTC 
AGAGAGCCGT ATCTCTTTGT GACCTACTTC AACTCTCTGG ATGTTATTGA GATTCAGGGA 
CACGCAGCTC TTGGGCCTCC CAGTCTGGTT CACCTGGACA TTCCTAACCC ACGTTATCTG 
GGTCCAGCCA TATCGTCTGG TGCCATCTAT CTGGCCTCGT CCTACCAGAA CAAACTGCGG 
GTGATCTGCT GTAAGGGCAG TCTGGTGCGG GAGTCTGGAG AGCTGCAGAG GACCGGCTCC 
AGCAGGAGCA GCCCCAACAA GCGCGGTCCA CCAACATACA ACGAGCACAT CTCCAAACGT 
CTGGCCTCGA GTCCTGGAGC CCAGGAGGGG CCCCTGCACC GCGAGCCCAG CACTCCTCAC 
CGCTACCGCG AGGGCCGCAC CGAGTTCCGG AGAGACAAAT CTCCAGCCCG GCCCCTGGAG 
CGAGAGAAAT CCCCGGGCCG TCCGGTGCTA GACAGCCGCA GAGAGAGGTC TCCCGGTCGC 
TTCGAGAGCA GCGGCAGCGA GAGAGACCGC GAGCGGACAC GACTGCACAC TAGCTCTGTA 
CGCACCCAGC TGACCCCCGT CAACAAGGTT TGGGACCAGT CTTCGGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212890.1
CDS	125..5095
Translation	MEQEISKVQRKMSDLESVLQQKDVELKASETQRNILEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSNKLQEIKEQEYHAQLEEMRVSIRQLEENLSAARRRSDLYESELKESRQTSEELKRKAVEYQQKIQKAKEQGKVEMEELLSKLQKTNGEQQVKIEELQEKLAKAVKASTEATELLQNVRQAKERMERDLERLQTKEDSSDSLRRRLRETEDGKKSLENQVKRLEMVERRESKLKDDIQTKSQQIQQMADKIMELEENLRDSQAMAQRMETQLEQKEKLFEDKIKVLETQMKADLAEKDLLESKQSTFEEESRERGKVISDQAATINAMESKMTSLEQRIAELSEANKLAANSSIYTQKNMKAQEEMISELRQQKFYLESQAGKLEAQNAKLEEHLEKLTQQEQTNKSRVLELEARLREIGLEHEEQKLEIKRQVSELTLSLQEREAQISSLQAARHALDSQLQQAKTELEETTAEAEEEITVLRAHRDEIQRKFDALRDSCAVITDLEEQLTQLTQENAELNRQNFYLSKQLDEATDENEERLQLKQDVERLRREVADREMHLNNQKQVTRQHTHTHTHTHTHRTEVADREMHLNNQKQNIETLKTTCTMLEEQVMELETLNDELMEKERQWENWRSALDEEKNQAERRARDLQRQLDSEKQNRLRAEQRNSESRQAVELAVKEHKAEILVLQQALKDQKLKAEGLSDTLGDLEKKHGMLEMNARSLQQKLETEREMKQRLMDEQAKLQQQLDMQKSHIFRLTQGLQDALDQTDLLKTERTDLEYQLENIQAAYSHEKVKMEGTITQQTKLIDFLQAKVEHPSKKKKGLFGGRRREDLLAALAAQGQTQVPVVPAATPVPLQYSDLKAALDKERARCSELEDALQKTRLELREVQFNKALEHSSSPATPGLARQQLMMSTKSNKSPEHQSSGGLMSSNSSRGRKDNANPEEIRRVTSEKYTRRVKDRIHHNTPHRFTQGLNMRAARCTVCLDTVHFGRQAATCIECHALCHPKCSPYLPATCGVPTEYALHLSEALCREKGSTSGLKDMGGHMRLEGWLKQPRNGKRGQGWERKYVVLDGYKVITFETEPRDESVKPLDEFELCLSDGEVTVHSAVGSSELPNTAKTDVPYVLKLESSPQSTCWPGQTLYLMTSGYSEKQRWVAVLEAIVASSRGAREKAEADAKLLGNSLLKLEGDDRLDINCTLPLTDQIVLVGSEEGLYALNVIKNSLTHIPGLGSVFQIHIIKDHDKLLMIVGDERALCLVEIKKVKQSLSQSHLPTLPEIVPYIFETVKGCHLFASGRIENGPCICAAMPNKVTILRYNDNLNKFCIRKEIETLEPCSCIHFTSYSIIIGTNKFYEIEMKQCVLDEFLDKNDVSLASAVFTGSSHSFPISIVQVSSTPQKEEYLLCFHEIGVFVDAYGRRSRPDDLKWSRLPLTFAFREPYLFVTYFNSLDVIEIQGHAALGPPSLVHLDIPNPRYLGPAISSGAIYLASSYQNKLRVICCKGSLVRESGELQRTGSSRSSPNKRGPPTYNEHISKRLASSPGAQEGPLHREPSTPHRYREGRTEFRRDKSPARPLEREKSPGRPVLDSRRERSPGRFESSGSERDRERTRLHTSSVRTQLTPVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_017357401.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357401.1

ORF Insert Sequence:

ATGGAGCAGG AGATTTCCAA AGTGCAGCGT AAGATGTCAG ACTTGGAGTC AGTTTTGCAG 
CAGAAAGACG TGGAGCTCAA GGCTTCAGAA ACCCAGAGAA ACATCTTAGA GCAAGACCTG 
GCAACCTATA TCACCGAATG CAGCAGTTTG AAGCGCAGTT TGGAGCAGGC CCGCATGGAG 
GTTTCGCAGG AAGATGATAA AGCTCTTCAG CTGCTGCACG ACATTCGAGA GCAGAGCAAC 
AAACTGCAGG AAATCAAAGA ACAGGAGTAC CACGCTCAGC TGGAGGAGAT GCGAGTGTCT 
ATTCGTCAGC TGGAAGAGAA TCTGTCGGCC GCTCGTCGCC GCAGTGACCT GTACGAGTCT 
GAGCTCAAAG AGTCCCGTCA GACCAGTGAG GAGCTCAAGA GGAAGGCAGT GGAGTATCAG 
CAGAAGATCC AGAAGGCTAA AGAACAAGGG AAAGTTGAGA TGGAGGAGCT TCTGAGCAAA 
CTGCAGAAGA CCAATGGAGA GCAACAAGTA AAGATTGAAG AGCTCCAGGA GAAGCTTGCT 
AAGGCAGTGA AGGCGAGTAC TGAAGCCACT GAACTCCTCC AGAATGTCCG TCAGGCTAAA 
GAGCGAATGG AGCGAGACCT GGAGCGTCTG CAGACCAAAG AGGACTCGTC CGACAGCCTG 
CGCAGACGCT TACGAGAGAC TGAGGATGGC AAAAAATCCC TGGAGAACCA GGTGAAGAGG 
CTGGAGATGG TGGAGCGGAG AGAATCCAAA CTGAAAGACG ACATTCAGAC CAAATCGCAG 
CAGATCCAGC AGATGGCCGA CAAAATCATG GAGCTTGAAG AAAATTTGCG GGACTCGCAG 
GCGATGGCGC AGCGCATGGA AACACAGCTG GAGCAGAAGG AAAAGCTTTT TGAAGACAAG 
ATCAAGGTTT TAGAAACTCA AATGAAGGCT GATTTGGCTG AGAAGGATTT GCTGGAGTCC 
AAACAGAGCA CTTTTGAAGA GGAGTCTCGC GAACGGGGAA AAGTGATCAG CGACCAGGCA 
GCGACGATCA ATGCCATGGA GTCAAAGATG ACCAGTCTGG AGCAGAGGAT CGCTGAGCTG 
TCCGAAGCCA ACAAACTGGC AGCAAACAGC AGCATTTACA CACAGAAGAA CATGAAAGCC 
CAGGAGGAGA TGATTTCTGA ACTCAGACAG CAGAAGTTTT ATTTGGAGTC TCAGGCTGGA 
AAACTGGAGG CGCAGAATGC AAAGCTGGAG GAGCATCTGG AGAAACTCAC CCAACAAGAG 
CAGACAAACA AGAGCCGAGT GCTGGAGCTG GAAGCCAGGC TGCGAGAGAT CGGCCTAGAG 
CATGAGGAAC AGAAGCTGGA GATCAAGCGT CAGGTGTCGG AGCTCACGTT GTCTCTGCAG 
GAGAGAGAAG CTCAGATCAG CAGCCTGCAA GCGGCACGCC ACGCTCTCGA CAGCCAACTA 
CAGCAGGCCA AAACTGAGCT GGAGGAGACC ACCGCCGAGG CCGAAGAGGA GATCACCGTC 
CTGAGGGCCC ATCGGGATGA AATCCAACGC AAGTTTGATG CCCTGCGAGA CAGCTGTGCG 
GTGATCACGG ATCTGGAGGA GCAGTTGACG CAGCTCACGC AAGAAAACGC GGAGCTGAAC 
CGGCAGAACT TCTACCTGTC CAAACAGCTG GACGAGGCCA CAGACGAGAA CGAGGAGCGC 
CTGCAGCTCA AGCAGGACGT GGAGCGCCTC AGGAGAGAGG TGGCAGACCG AGAGATGCAC 
CTCAACAACC AGAAACAGGT GACACGGCAA CACACACACA CACACACACA CACACACACA 
CACAGGACAG AGGTGGCAGA CCGAGAGATG CACCTCAACA ACCAGAAACA GAACATCGAG 
ACGCTGAAGA CGACATGTAC AATGCTGGAG GAGCAGGTTA TGGAGCTGGA GACTCTGAAT 
GATGAGCTGA TGGAGAAGGA GAGGCAGTGG GAGAACTGGA GATCTGCTCT GGACGAGGAG 
AAAAACCAGG CCGAGCGCAG AGCCAGAGAC CTGCAAAGAC AGCTGGACAG CGAGAAGCAG 
AACCGACTGC GTGCAGAACA GAGAAACTCT GAGTCTCGGC AGGCCGTGGA GCTGGCGGTG 
AAAGAGCACA AAGCTGAAAT CCTGGTTCTG CAGCAGGCGC TCAAAGACCA GAAACTCAAG 
GCTGAGGGCC TTTCTGACAC TCTGGGTGAT CTGGAGAAGA AGCACGGCAT GCTGGAGATG 
AACGCTCGCA GCCTGCAGCA GAAGCTGGAG ACGGAGAGAG AGATGAAGCA GAGACTCATG 
GACGAGCAAG CTAAACTGCA GCAGCAGCTG GACATGCAGA AGAGCCACAT CTTCAGACTG 
ACTCAAGGAC TGCAGGATGC GCTGGACCAG ACCGACCTGC TGAAGACCGA GCGCACAGAC 
CTGGAGTACC AGCTGGAAAA CATACAGGCG GCGTATTCTC ACGAGAAGGT GAAGATGGAG 
GGGACGATCA CCCAGCAGAC CAAGCTCATA GACTTCCTGC AGGCCAAGGT AGAGCACCCC 
TCTAAGAAGA AAAAGGGGCT GTTTGGTGGC CGGCGGCGGG AGGATCTGCT AGCGGCGCTG 
GCGGCTCAGG GACAGACACA GGTGCCTGTG GTGCCCGCCG CCACTCCTGT GCCCCTGCAG 
TACAGCGACC TGAAGGCGGC GCTGGATAAA GAGCGAGCCC GATGCTCCGA GCTGGAGGAC 
GCCCTGCAGA AAACACGCCT GGAGCTCAGA GAAGTTCAGT TTAATAAAGC TCTGGAGCAC 
AGCTCTTCTC CAGCCACTCC AGGTTTGGCC CGTCAGCAGC TCATGATGTC CACCAAGTCC 
AACAAGTCTC CAGAGCACCA GTCCAGCGGC GGCCTGATGT CCTCAAACTC CAGCCGCGGA 
CGCAAAGACA ACGCCAATCC TGAAGAAATA AGGAGAGTCA CTTCTGAAAA GTACACCAGA 
CGTGTGAAGG ACCGGATTCA TCACAACACT CCTCACCGCT TCACGCAGGG GCTCAATATG 
AGAGCGGCTC GCTGTACTGT GTGTCTGGAC ACAGTCCATT TTGGACGTCA GGCTGCAACA 
TGCATTGAAT GTCACGCGTT GTGTCACCCA AAATGCTCCC CGTACCTCCC TGCCACCTGC 
GGCGTTCCCA CTGAATACGC GCTGCACCTC TCAGAGGCTC TGTGTCGGGA GAAGGGAAGC 
ACTTCCGGCC TGAAAGATAT GGGTGGACAT ATGAGGCTTG AAGGATGGCT GAAGCAGCCC 
AGGAACGGGA AGCGCGGGCA GGGCTGGGAG AGGAAGTATG TGGTGCTGGA TGGATATAAA 
GTGATTACAT TCGAGACAGA ACCAAGAGAC GAGTCAGTGA AGCCGCTGGA TGAGTTTGAG 
CTGTGTCTCT CTGATGGAGA AGTGACCGTT CATTCTGCTG TGGGTTCCTC TGAGCTCCCC 
AACACCGCTA AAACAGATGT GCCGTACGTG CTGAAGCTGG AGTCGTCTCC TCAGTCCACA 
TGCTGGCCCG GTCAGACTCT GTACCTCATG ACCTCGGGAT ACTCAGAGAA GCAGCGCTGG 
GTGGCAGTGC TGGAAGCCAT CGTGGCCAGC AGTCGCGGAG CCAGAGAGAA AGCTGAGGCA 
GATGCTAAAT TATTGGGTAA CTCTTTACTT AAGCTAGAAG GTGACGACCG TCTGGATATC 
AACTGCACCC TGCCGCTGAC CGACCAGATT GTGTTGGTGG GCTCTGAGGA GGGTCTCTAC 
GCACTGAATG TGATTAAGAA CTCCCTCACT CATATCCCGG GTCTGGGATC CGTGTTTCAG 
ATCCACATTA TCAAAGATCA TGACAAGCTG CTGATGATCG TGGGAGACGA GCGAGCGCTG 
TGTCTGGTGG AGATTAAGAA GGTGAAGCAG TCTTTATCCC AGTCTCACCT CCCCACACTG 
CCCGAGATTG TGCCGTATAT CTTTGAGACC GTCAAGGGCT GTCATCTGTT TGCCTCGGGC 
AGGATAGAAA ATGGCCCATG CATTTGTGCT GCGATGCCCA ATAAAGTCAC GATTCTACGA 
TACAATGACA ATCTTAACAA GTTCTGCATA CGGAAGGAGA TTGAAACTCT GGAGCCGTGC 
AGCTGCATCC ACTTCACCAG CTACAGCATC ATCATTGGTA CCAATAAATT CTACGAGATC 
GAGATGAAGC AGTGCGTTCT GGACGAGTTC CTGGACAAGA ATGACGTGTC GCTGGCGTCG 
GCTGTCTTCA CCGGATCCTC CCACAGTTTC CCCATCTCTA TCGTCCAGGT GTCCAGCACT 
CCGCAGAAGG AGGAATATCT GCTGTGTTTC CACGAGATCG GAGTTTTTGT GGATGCTTAC 
GGTCGAAGAA GTCGCCCTGA TGATCTGAAG TGGAGTCGAC TTCCCCTGAC GTTCGCCTTC 
AGAGAGCCGT ATCTCTTTGT GACCTACTTC AACTCTCTGG ATGTTATTGA GATTCAGGGA 
CACGCAGCTC TTGGGCCTCC CAGTCTGGTT CACCTGGACA TTCCTAACCC ACGTTATCTG 
GGTCCAGCCA TATCGTCTGG TGCCATCTAT CTGGCCTCGT CCTACCAGAA CAAACTGCGG 
GTGATCTGCT GTAAGGGCAG TCTGGTGCGG GAGTCTGGAG AGCTGCAGAG GACCGGCTCC 
AGCAGGAGCA GCCCCAACAA GCGCGGTCCA CCAACATACA ACGAGCACAT CTCCAAACGT 
CTGGCCTCGA GTCCTGGAGC CCAGGAGGGG CCCCTGCACC GCGAGCCCAG CACTCCTCAC 
CGCTACCGCG AGGGCCGCAC CGAGTTCCGG AGAGACAAAT CTCCAGCCCG GCCCCTGGAG 
CGAGAGAAAT CCCCGGGCCG TCCGGTGCTA GACAGCCGCA GAGAGAGGTC TCCCGGTCGC 
TTCGAGAGCA GCGGCAGCGA GAGAGACCGC GAGCGGACAC GACTGCACAC TAGCTCTGTA 
CGCACCCAGC TGACCCCCGT CAACAAGGTT TGGGACCAGT CTTCGGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100333880 ( XM_017357400.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212889.1 Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X1, mRNA.

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CloneID	ODa60005
Clone ID Related Accession (Same CDS sequence)	XM_017357401.1 , XM_017357400.1 , XM_017357400.2
Accession Version	XM_017357400.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	citron Rho-interacting kinase-like isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG AGATTTCCAA AGTGCAGCGT AAGATGTCAG ACTTGGAGTC AGTTTTGCAG 
CAGAAAGACG TGGAGCTCAA GGCTTCAGAA ACCCAGAGAA ACATCTTAGA GCAAGACCTG 
GCAACCTATA TCACCGAATG CAGCAGTTTG AAGCGCAGTT TGGAGCAGGC CCGCATGGAG 
GTTTCGCAGG AAGATGATAA AGCTCTTCAG CTGCTGCACG ACATTCGAGA GCAGAGCAAC 
AAACTGCAGG AAATCAAAGA ACAGGAGTAC CACGCTCAGC TGGAGGAGAT GCGAGTGTCT 
ATTCGTCAGC TGGAAGAGAA TCTGTCGGCC GCTCGTCGCC GCAGTGACCT GTACGAGTCT 
GAGCTCAAAG AGTCCCGTCA GACCAGTGAG GAGCTCAAGA GGAAGGCAGT GGAGTATCAG 
CAGAAGATCC AGAAGGCTAA AGAACAAGGG AAAGTTGAGA TGGAGGAGCT TCTGAGCAAA 
CTGCAGAAGA CCAATGGAGA GCAACAAGTA AAGATTGAAG AGCTCCAGGA GAAGCTTGCT 
AAGGCAGTGA AGGCGAGTAC TGAAGCCACT GAACTCCTCC AGAATGTCCG TCAGGCTAAA 
GAGCGAATGG AGCGAGACCT GGAGCGTCTG CAGACCAAAG AGGACTCGTC CGACAGCCTG 
CGCAGACGCT TACGAGAGAC TGAGGATGGC AAAAAATCCC TGGAGAACCA GGTGAAGAGG 
CTGGAGATGG TGGAGCGGAG AGAATCCAAA CTGAAAGACG ACATTCAGAC CAAATCGCAG 
CAGATCCAGC AGATGGCCGA CAAAATCATG GAGCTTGAAG AAAATTTGCG GGACTCGCAG 
GCGATGGCGC AGCGCATGGA AACACAGCTG GAGCAGAAGG AAAAGCTTTT TGAAGACAAG 
ATCAAGGTTT TAGAAACTCA AATGAAGGCT GATTTGGCTG AGAAGGATTT GCTGGAGTCC 
AAACAGAGCA CTTTTGAAGA GGAGTCTCGC GAACGGGGAA AAGTGATCAG CGACCAGGCA 
GCGACGATCA ATGCCATGGA GTCAAAGATG ACCAGTCTGG AGCAGAGGAT CGCTGAGCTG 
TCCGAAGCCA ACAAACTGGC AGCAAACAGC AGCATTTACA CACAGAAGAA CATGAAAGCC 
CAGGAGGAGA TGATTTCTGA ACTCAGACAG CAGAAGTTTT ATTTGGAGTC TCAGGCTGGA 
AAACTGGAGG CGCAGAATGC AAAGCTGGAG GAGCATCTGG AGAAACTCAC CCAACAAGAG 
CAGACAAACA AGAGCCGAGT GCTGGAGCTG GAAGCCAGGC TGCGAGAGAT CGGCCTAGAG 
CATGAGGAAC AGAAGCTGGA GATCAAGCGT CAGGTGTCGG AGCTCACGTT GTCTCTGCAG 
GAGAGAGAAG CTCAGATCAG CAGCCTGCAA GCGGCACGCC ACGCTCTCGA CAGCCAACTA 
CAGCAGGCCA AAACTGAGCT GGAGGAGACC ACCGCCGAGG CCGAAGAGGA GATCACCGTC 
CTGAGGGCCC ATCGGGATGA AATCCAACGC AAGTTTGATG CCCTGCGAGA CAGCTGTGCG 
GTGATCACGG ATCTGGAGGA GCAGTTGACG CAGCTCACGC AAGAAAACGC GGAGCTGAAC 
CGGCAGAACT TCTACCTGTC CAAACAGCTG GACGAGGCCA CAGACGAGAA CGAGGAGCGC 
CTGCAGCTCA AGCAGGACGT GGAGCGCCTC AGGAGAGAGG TGGCAGACCG AGAGATGCAC 
CTCAACAACC AGAAACAGGT GACACGGCAA CACACACACA CACACACACA CACACACACA 
CACAGGACAG AGGTGGCAGA CCGAGAGATG CACCTCAACA ACCAGAAACA GAACATCGAG 
ACGCTGAAGA CGACATGTAC AATGCTGGAG GAGCAGGTTA TGGAGCTGGA GACTCTGAAT 
GATGAGCTGA TGGAGAAGGA GAGGCAGTGG GAGAACTGGA GATCTGCTCT GGACGAGGAG 
AAAAACCAGG CCGAGCGCAG AGCCAGAGAC CTGCAAAGAC AGCTGGACAG CGAGAAGCAG 
AACCGACTGC GTGCAGAACA GAGAAACTCT GAGTCTCGGC AGGCCGTGGA GCTGGCGGTG 
AAAGAGCACA AAGCTGAAAT CCTGGTTCTG CAGCAGGCGC TCAAAGACCA GAAACTCAAG 
GCTGAGGGCC TTTCTGACAC TCTGGGTGAT CTGGAGAAGA AGCACGGCAT GCTGGAGATG 
AACGCTCGCA GCCTGCAGCA GAAGCTGGAG ACGGAGAGAG AGATGAAGCA GAGACTCATG 
GACGAGCAAG CTAAACTGCA GCAGCAGCTG GACATGCAGA AGAGCCACAT CTTCAGACTG 
ACTCAAGGAC TGCAGGATGC GCTGGACCAG ACCGACCTGC TGAAGACCGA GCGCACAGAC 
CTGGAGTACC AGCTGGAAAA CATACAGGCG GCGTATTCTC ACGAGAAGGT GAAGATGGAG 
GGGACGATCA CCCAGCAGAC CAAGCTCATA GACTTCCTGC AGGCCAAGGT AGAGCACCCC 
TCTAAGAAGA AAAAGGGGCT GTTTGGTGGC CGGCGGCGGG AGGATCTGCT AGCGGCGCTG 
GCGGCTCAGG GACAGACACA GGTGCCTGTG GTGCCCGCCG CCACTCCTGT GCCCCTGCAG 
TACAGCGACC TGAAGGCGGC GCTGGATAAA GAGCGAGCCC GATGCTCCGA GCTGGAGGAC 
GCCCTGCAGA AAACACGCCT GGAGCTCAGA GAAGTTCAGT TTAATAAAGC TCTGGAGCAC 
AGCTCTTCTC CAGCCACTCC AGGTTTGGCC CGTCAGCAGC TCATGATGTC CACCAAGTCC 
AACAAGTCTC CAGAGCACCA GTCCAGCGGC GGCCTGATGT CCTCAAACTC CAGCCGCGGA 
CGCAAAGACA ACGCCAATCC TGAAGAAATA AGGAGAGTCA CTTCTGAAAA GTACACCAGA 
CGTGTGAAGG ACCGGATTCA TCACAACACT CCTCACCGCT TCACGCAGGG GCTCAATATG 
AGAGCGGCTC GCTGTACTGT GTGTCTGGAC ACAGTCCATT TTGGACGTCA GGCTGCAACA 
TGCATTGAAT GTCACGCGTT GTGTCACCCA AAATGCTCCC CGTACCTCCC TGCCACCTGC 
GGCGTTCCCA CTGAATACGC GCTGCACCTC TCAGAGGCTC TGTGTCGGGA GAAGGGAAGC 
ACTTCCGGCC TGAAAGATAT GGGTGGACAT ATGAGGCTTG AAGGATGGCT GAAGCAGCCC 
AGGAACGGGA AGCGCGGGCA GGGCTGGGAG AGGAAGTATG TGGTGCTGGA TGGATATAAA 
GTGATTACAT TCGAGACAGA ACCAAGAGAC GAGTCAGTGA AGCCGCTGGA TGAGTTTGAG 
CTGTGTCTCT CTGATGGAGA AGTGACCGTT CATTCTGCTG TGGGTTCCTC TGAGCTCCCC 
AACACCGCTA AAACAGATGT GCCGTACGTG CTGAAGCTGG AGTCGTCTCC TCAGTCCACA 
TGCTGGCCCG GTCAGACTCT GTACCTCATG ACCTCGGGAT ACTCAGAGAA GCAGCGCTGG 
GTGGCAGTGC TGGAAGCCAT CGTGGCCAGC AGTCGCGGAG CCAGAGAGAA AGCTGAGGCA 
GATGCTAAAT TATTGGGTAA CTCTTTACTT AAGCTAGAAG GTGACGACCG TCTGGATATC 
AACTGCACCC TGCCGCTGAC CGACCAGATT GTGTTGGTGG GCTCTGAGGA GGGTCTCTAC 
GCACTGAATG TGATTAAGAA CTCCCTCACT CATATCCCGG GTCTGGGATC CGTGTTTCAG 
ATCCACATTA TCAAAGATCA TGACAAGCTG CTGATGATCG TGGGAGACGA GCGAGCGCTG 
TGTCTGGTGG AGATTAAGAA GGTGAAGCAG TCTTTATCCC AGTCTCACCT CCCCACACTG 
CCCGAGATTG TGCCGTATAT CTTTGAGACC GTCAAGGGCT GTCATCTGTT TGCCTCGGGC 
AGGATAGAAA ATGGCCCATG CATTTGTGCT GCGATGCCCA ATAAAGTCAC GATTCTACGA 
TACAATGACA ATCTTAACAA GTTCTGCATA CGGAAGGAGA TTGAAACTCT GGAGCCGTGC 
AGCTGCATCC ACTTCACCAG CTACAGCATC ATCATTGGTA CCAATAAATT CTACGAGATC 
GAGATGAAGC AGTGCGTTCT GGACGAGTTC CTGGACAAGA ATGACGTGTC GCTGGCGTCG 
GCTGTCTTCA CCGGATCCTC CCACAGTTTC CCCATCTCTA TCGTCCAGGT GTCCAGCACT 
CCGCAGAAGG AGGAATATCT GCTGTGTTTC CACGAGATCG GAGTTTTTGT GGATGCTTAC 
GGTCGAAGAA GTCGCCCTGA TGATCTGAAG TGGAGTCGAC TTCCCCTGAC GTTCGCCTTC 
AGAGAGCCGT ATCTCTTTGT GACCTACTTC AACTCTCTGG ATGTTATTGA GATTCAGGGA 
CACGCAGCTC TTGGGCCTCC CAGTCTGGTT CACCTGGACA TTCCTAACCC ACGTTATCTG 
GGTCCAGCCA TATCGTCTGG TGCCATCTAT CTGGCCTCGT CCTACCAGAA CAAACTGCGG 
GTGATCTGCT GTAAGGGCAG TCTGGTGCGG GAGTCTGGAG AGCTGCAGAG GACCGGCTCC 
AGCAGGAGCA GCCCCAACAA GCGCGGTCCA CCAACATACA ACGAGCACAT CTCCAAACGT 
CTGGCCTCGA GTCCTGGAGC CCAGGAGGGG CCCCTGCACC GCGAGCCCAG CACTCCTCAC 
CGCTACCGCG AGGGCCGCAC CGAGTTCCGG AGAGACAAAT CTCCAGCCCG GCCCCTGGAG 
CGAGAGAAAT CCCCGGGCCG TCCGGTGCTA GACAGCCGCA GAGAGAGGTC TCCCGGTCGC 
TTCGAGAGCA GCGGCAGCGA GAGAGACCGC GAGCGGACAC GACTGCACAC TAGCTCTGTA 
CGCACCCAGC TGACCCCCGT CAACAAGGTT TGGGACCAGT CTTCGGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212889.1
CDS	434..5404
Translation	MEQEISKVQRKMSDLESVLQQKDVELKASETQRNILEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSNKLQEIKEQEYHAQLEEMRVSIRQLEENLSAARRRSDLYESELKESRQTSEELKRKAVEYQQKIQKAKEQGKVEMEELLSKLQKTNGEQQVKIEELQEKLAKAVKASTEATELLQNVRQAKERMERDLERLQTKEDSSDSLRRRLRETEDGKKSLENQVKRLEMVERRESKLKDDIQTKSQQIQQMADKIMELEENLRDSQAMAQRMETQLEQKEKLFEDKIKVLETQMKADLAEKDLLESKQSTFEEESRERGKVISDQAATINAMESKMTSLEQRIAELSEANKLAANSSIYTQKNMKAQEEMISELRQQKFYLESQAGKLEAQNAKLEEHLEKLTQQEQTNKSRVLELEARLREIGLEHEEQKLEIKRQVSELTLSLQEREAQISSLQAARHALDSQLQQAKTELEETTAEAEEEITVLRAHRDEIQRKFDALRDSCAVITDLEEQLTQLTQENAELNRQNFYLSKQLDEATDENEERLQLKQDVERLRREVADREMHLNNQKQVTRQHTHTHTHTHTHRTEVADREMHLNNQKQNIETLKTTCTMLEEQVMELETLNDELMEKERQWENWRSALDEEKNQAERRARDLQRQLDSEKQNRLRAEQRNSESRQAVELAVKEHKAEILVLQQALKDQKLKAEGLSDTLGDLEKKHGMLEMNARSLQQKLETEREMKQRLMDEQAKLQQQLDMQKSHIFRLTQGLQDALDQTDLLKTERTDLEYQLENIQAAYSHEKVKMEGTITQQTKLIDFLQAKVEHPSKKKKGLFGGRRREDLLAALAAQGQTQVPVVPAATPVPLQYSDLKAALDKERARCSELEDALQKTRLELREVQFNKALEHSSSPATPGLARQQLMMSTKSNKSPEHQSSGGLMSSNSSRGRKDNANPEEIRRVTSEKYTRRVKDRIHHNTPHRFTQGLNMRAARCTVCLDTVHFGRQAATCIECHALCHPKCSPYLPATCGVPTEYALHLSEALCREKGSTSGLKDMGGHMRLEGWLKQPRNGKRGQGWERKYVVLDGYKVITFETEPRDESVKPLDEFELCLSDGEVTVHSAVGSSELPNTAKTDVPYVLKLESSPQSTCWPGQTLYLMTSGYSEKQRWVAVLEAIVASSRGAREKAEADAKLLGNSLLKLEGDDRLDINCTLPLTDQIVLVGSEEGLYALNVIKNSLTHIPGLGSVFQIHIIKDHDKLLMIVGDERALCLVEIKKVKQSLSQSHLPTLPEIVPYIFETVKGCHLFASGRIENGPCICAAMPNKVTILRYNDNLNKFCIRKEIETLEPCSCIHFTSYSIIIGTNKFYEIEMKQCVLDEFLDKNDVSLASAVFTGSSHSFPISIVQVSSTPQKEEYLLCFHEIGVFVDAYGRRSRPDDLKWSRLPLTFAFREPYLFVTYFNSLDVIEIQGHAALGPPSLVHLDIPNPRYLGPAISSGAIYLASSYQNKLRVICCKGSLVRESGELQRTGSSRSSPNKRGPPTYNEHISKRLASSPGAQEGPLHREPSTPHRYREGRTEFRRDKSPARPLEREKSPGRPVLDSRRERSPGRFESSGSERDRERTRLHTSSVRTQLTPVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_017357400.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357400.1

ORF Insert Sequence:

ATGGAGCAGG AGATTTCCAA AGTGCAGCGT AAGATGTCAG ACTTGGAGTC AGTTTTGCAG 
CAGAAAGACG TGGAGCTCAA GGCTTCAGAA ACCCAGAGAA ACATCTTAGA GCAAGACCTG 
GCAACCTATA TCACCGAATG CAGCAGTTTG AAGCGCAGTT TGGAGCAGGC CCGCATGGAG 
GTTTCGCAGG AAGATGATAA AGCTCTTCAG CTGCTGCACG ACATTCGAGA GCAGAGCAAC 
AAACTGCAGG AAATCAAAGA ACAGGAGTAC CACGCTCAGC TGGAGGAGAT GCGAGTGTCT 
ATTCGTCAGC TGGAAGAGAA TCTGTCGGCC GCTCGTCGCC GCAGTGACCT GTACGAGTCT 
GAGCTCAAAG AGTCCCGTCA GACCAGTGAG GAGCTCAAGA GGAAGGCAGT GGAGTATCAG 
CAGAAGATCC AGAAGGCTAA AGAACAAGGG AAAGTTGAGA TGGAGGAGCT TCTGAGCAAA 
CTGCAGAAGA CCAATGGAGA GCAACAAGTA AAGATTGAAG AGCTCCAGGA GAAGCTTGCT 
AAGGCAGTGA AGGCGAGTAC TGAAGCCACT GAACTCCTCC AGAATGTCCG TCAGGCTAAA 
GAGCGAATGG AGCGAGACCT GGAGCGTCTG CAGACCAAAG AGGACTCGTC CGACAGCCTG 
CGCAGACGCT TACGAGAGAC TGAGGATGGC AAAAAATCCC TGGAGAACCA GGTGAAGAGG 
CTGGAGATGG TGGAGCGGAG AGAATCCAAA CTGAAAGACG ACATTCAGAC CAAATCGCAG 
CAGATCCAGC AGATGGCCGA CAAAATCATG GAGCTTGAAG AAAATTTGCG GGACTCGCAG 
GCGATGGCGC AGCGCATGGA AACACAGCTG GAGCAGAAGG AAAAGCTTTT TGAAGACAAG 
ATCAAGGTTT TAGAAACTCA AATGAAGGCT GATTTGGCTG AGAAGGATTT GCTGGAGTCC 
AAACAGAGCA CTTTTGAAGA GGAGTCTCGC GAACGGGGAA AAGTGATCAG CGACCAGGCA 
GCGACGATCA ATGCCATGGA GTCAAAGATG ACCAGTCTGG AGCAGAGGAT CGCTGAGCTG 
TCCGAAGCCA ACAAACTGGC AGCAAACAGC AGCATTTACA CACAGAAGAA CATGAAAGCC 
CAGGAGGAGA TGATTTCTGA ACTCAGACAG CAGAAGTTTT ATTTGGAGTC TCAGGCTGGA 
AAACTGGAGG CGCAGAATGC AAAGCTGGAG GAGCATCTGG AGAAACTCAC CCAACAAGAG 
CAGACAAACA AGAGCCGAGT GCTGGAGCTG GAAGCCAGGC TGCGAGAGAT CGGCCTAGAG 
CATGAGGAAC AGAAGCTGGA GATCAAGCGT CAGGTGTCGG AGCTCACGTT GTCTCTGCAG 
GAGAGAGAAG CTCAGATCAG CAGCCTGCAA GCGGCACGCC ACGCTCTCGA CAGCCAACTA 
CAGCAGGCCA AAACTGAGCT GGAGGAGACC ACCGCCGAGG CCGAAGAGGA GATCACCGTC 
CTGAGGGCCC ATCGGGATGA AATCCAACGC AAGTTTGATG CCCTGCGAGA CAGCTGTGCG 
GTGATCACGG ATCTGGAGGA GCAGTTGACG CAGCTCACGC AAGAAAACGC GGAGCTGAAC 
CGGCAGAACT TCTACCTGTC CAAACAGCTG GACGAGGCCA CAGACGAGAA CGAGGAGCGC 
CTGCAGCTCA AGCAGGACGT GGAGCGCCTC AGGAGAGAGG TGGCAGACCG AGAGATGCAC 
CTCAACAACC AGAAACAGGT GACACGGCAA CACACACACA CACACACACA CACACACACA 
CACAGGACAG AGGTGGCAGA CCGAGAGATG CACCTCAACA ACCAGAAACA GAACATCGAG 
ACGCTGAAGA CGACATGTAC AATGCTGGAG GAGCAGGTTA TGGAGCTGGA GACTCTGAAT 
GATGAGCTGA TGGAGAAGGA GAGGCAGTGG GAGAACTGGA GATCTGCTCT GGACGAGGAG 
AAAAACCAGG CCGAGCGCAG AGCCAGAGAC CTGCAAAGAC AGCTGGACAG CGAGAAGCAG 
AACCGACTGC GTGCAGAACA GAGAAACTCT GAGTCTCGGC AGGCCGTGGA GCTGGCGGTG 
AAAGAGCACA AAGCTGAAAT CCTGGTTCTG CAGCAGGCGC TCAAAGACCA GAAACTCAAG 
GCTGAGGGCC TTTCTGACAC TCTGGGTGAT CTGGAGAAGA AGCACGGCAT GCTGGAGATG 
AACGCTCGCA GCCTGCAGCA GAAGCTGGAG ACGGAGAGAG AGATGAAGCA GAGACTCATG 
GACGAGCAAG CTAAACTGCA GCAGCAGCTG GACATGCAGA AGAGCCACAT CTTCAGACTG 
ACTCAAGGAC TGCAGGATGC GCTGGACCAG ACCGACCTGC TGAAGACCGA GCGCACAGAC 
CTGGAGTACC AGCTGGAAAA CATACAGGCG GCGTATTCTC ACGAGAAGGT GAAGATGGAG 
GGGACGATCA CCCAGCAGAC CAAGCTCATA GACTTCCTGC AGGCCAAGGT AGAGCACCCC 
TCTAAGAAGA AAAAGGGGCT GTTTGGTGGC CGGCGGCGGG AGGATCTGCT AGCGGCGCTG 
GCGGCTCAGG GACAGACACA GGTGCCTGTG GTGCCCGCCG CCACTCCTGT GCCCCTGCAG 
TACAGCGACC TGAAGGCGGC GCTGGATAAA GAGCGAGCCC GATGCTCCGA GCTGGAGGAC 
GCCCTGCAGA AAACACGCCT GGAGCTCAGA GAAGTTCAGT TTAATAAAGC TCTGGAGCAC 
AGCTCTTCTC CAGCCACTCC AGGTTTGGCC CGTCAGCAGC TCATGATGTC CACCAAGTCC 
AACAAGTCTC CAGAGCACCA GTCCAGCGGC GGCCTGATGT CCTCAAACTC CAGCCGCGGA 
CGCAAAGACA ACGCCAATCC TGAAGAAATA AGGAGAGTCA CTTCTGAAAA GTACACCAGA 
CGTGTGAAGG ACCGGATTCA TCACAACACT CCTCACCGCT TCACGCAGGG GCTCAATATG 
AGAGCGGCTC GCTGTACTGT GTGTCTGGAC ACAGTCCATT TTGGACGTCA GGCTGCAACA 
TGCATTGAAT GTCACGCGTT GTGTCACCCA AAATGCTCCC CGTACCTCCC TGCCACCTGC 
GGCGTTCCCA CTGAATACGC GCTGCACCTC TCAGAGGCTC TGTGTCGGGA GAAGGGAAGC 
ACTTCCGGCC TGAAAGATAT GGGTGGACAT ATGAGGCTTG AAGGATGGCT GAAGCAGCCC 
AGGAACGGGA AGCGCGGGCA GGGCTGGGAG AGGAAGTATG TGGTGCTGGA TGGATATAAA 
GTGATTACAT TCGAGACAGA ACCAAGAGAC GAGTCAGTGA AGCCGCTGGA TGAGTTTGAG 
CTGTGTCTCT CTGATGGAGA AGTGACCGTT CATTCTGCTG TGGGTTCCTC TGAGCTCCCC 
AACACCGCTA AAACAGATGT GCCGTACGTG CTGAAGCTGG AGTCGTCTCC TCAGTCCACA 
TGCTGGCCCG GTCAGACTCT GTACCTCATG ACCTCGGGAT ACTCAGAGAA GCAGCGCTGG 
GTGGCAGTGC TGGAAGCCAT CGTGGCCAGC AGTCGCGGAG CCAGAGAGAA AGCTGAGGCA 
GATGCTAAAT TATTGGGTAA CTCTTTACTT AAGCTAGAAG GTGACGACCG TCTGGATATC 
AACTGCACCC TGCCGCTGAC CGACCAGATT GTGTTGGTGG GCTCTGAGGA GGGTCTCTAC 
GCACTGAATG TGATTAAGAA CTCCCTCACT CATATCCCGG GTCTGGGATC CGTGTTTCAG 
ATCCACATTA TCAAAGATCA TGACAAGCTG CTGATGATCG TGGGAGACGA GCGAGCGCTG 
TGTCTGGTGG AGATTAAGAA GGTGAAGCAG TCTTTATCCC AGTCTCACCT CCCCACACTG 
CCCGAGATTG TGCCGTATAT CTTTGAGACC GTCAAGGGCT GTCATCTGTT TGCCTCGGGC 
AGGATAGAAA ATGGCCCATG CATTTGTGCT GCGATGCCCA ATAAAGTCAC GATTCTACGA 
TACAATGACA ATCTTAACAA GTTCTGCATA CGGAAGGAGA TTGAAACTCT GGAGCCGTGC 
AGCTGCATCC ACTTCACCAG CTACAGCATC ATCATTGGTA CCAATAAATT CTACGAGATC 
GAGATGAAGC AGTGCGTTCT GGACGAGTTC CTGGACAAGA ATGACGTGTC GCTGGCGTCG 
GCTGTCTTCA CCGGATCCTC CCACAGTTTC CCCATCTCTA TCGTCCAGGT GTCCAGCACT 
CCGCAGAAGG AGGAATATCT GCTGTGTTTC CACGAGATCG GAGTTTTTGT GGATGCTTAC 
GGTCGAAGAA GTCGCCCTGA TGATCTGAAG TGGAGTCGAC TTCCCCTGAC GTTCGCCTTC 
AGAGAGCCGT ATCTCTTTGT GACCTACTTC AACTCTCTGG ATGTTATTGA GATTCAGGGA 
CACGCAGCTC TTGGGCCTCC CAGTCTGGTT CACCTGGACA TTCCTAACCC ACGTTATCTG 
GGTCCAGCCA TATCGTCTGG TGCCATCTAT CTGGCCTCGT CCTACCAGAA CAAACTGCGG 
GTGATCTGCT GTAAGGGCAG TCTGGTGCGG GAGTCTGGAG AGCTGCAGAG GACCGGCTCC 
AGCAGGAGCA GCCCCAACAA GCGCGGTCCA CCAACATACA ACGAGCACAT CTCCAAACGT 
CTGGCCTCGA GTCCTGGAGC CCAGGAGGGG CCCCTGCACC GCGAGCCCAG CACTCCTCAC 
CGCTACCGCG AGGGCCGCAC CGAGTTCCGG AGAGACAAAT CTCCAGCCCG GCCCCTGGAG 
CGAGAGAAAT CCCCGGGCCG TCCGGTGCTA GACAGCCGCA GAGAGAGGTC TCCCGGTCGC 
TTCGAGAGCA GCGGCAGCGA GAGAGACCGC GAGCGGACAC GACTGCACAC TAGCTCTGTA 
CGCACCCAGC TGACCCCCGT CAACAAGGTT TGGGACCAGT CTTCGGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100333880 ( XM_017357400.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212889.1 Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X3, mRNA.

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CloneID	ODa60005
Clone ID Related Accession (Same CDS sequence)	XM_017357401.1 , XM_017357400.1 , XM_017357400.2
Accession Version	XM_017357400.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	citron Rho-interacting kinase-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017357400.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG AGATTTCCAA AGTGCAGCGT AAGATGTCAG ACTTGGAGTC AGTTTTGCAG 
CAGAAAGACG TGGAGCTCAA GGCTTCAGAA ACCCAGAGAA ACATCTTAGA GCAAGACCTG 
GCAACCTATA TCACCGAATG CAGCAGTTTG AAGCGCAGTT TGGAGCAGGC CCGCATGGAG 
GTTTCGCAGG AAGATGATAA AGCTCTTCAG CTGCTGCACG ACATTCGAGA GCAGAGCAAC 
AAACTGCAGG AAATCAAAGA ACAGGAGTAC CACGCTCAGC TGGAGGAGAT GCGAGTGTCT 
ATTCGTCAGC TGGAAGAGAA TCTGTCGGCC GCTCGTCGCC GCAGTGACCT GTACGAGTCT 
GAGCTCAAAG AGTCCCGTCA GACCAGTGAG GAGCTCAAGA GGAAGGCAGT GGAGTATCAG 
CAGAAGATCC AGAAGGCTAA AGAACAAGGG AAAGTTGAGA TGGAGGAGCT TCTGAGCAAA 
CTGCAGAAGA CCAATGGAGA GCAACAAGTA AAGATTGAAG AGCTCCAGGA GAAGCTTGCT 
AAGGCAGTGA AGGCGAGTAC TGAAGCCACT GAACTCCTCC AGAATGTCCG TCAGGCTAAA 
GAGCGAATGG AGCGAGACCT GGAGCGTCTG CAGACCAAAG AGGACTCGTC CGACAGCCTG 
CGCAGACGCT TACGAGAGAC TGAGGATGGC AAAAAATCCC TGGAGAACCA GGTGAAGAGG 
CTGGAGATGG TGGAGCGGAG AGAATCCAAA CTGAAAGACG ACATTCAGAC CAAATCGCAG 
CAGATCCAGC AGATGGCCGA CAAAATCATG GAGCTTGAAG AAAATTTGCG GGACTCGCAG 
GCGATGGCGC AGCGCATGGA AACACAGCTG GAGCAGAAGG AAAAGCTTTT TGAAGACAAG 
ATCAAGGTTT TAGAAACTCA AATGAAGGCT GATTTGGCTG AGAAGGATTT GCTGGAGTCC 
AAACAGAGCA CTTTTGAAGA GGAGTCTCGC GAACGGGGAA AAGTGATCAG CGACCAGGCA 
GCGACGATCA ATGCCATGGA GTCAAAGATG ACCAGTCTGG AGCAGAGGAT CGCTGAGCTG 
TCCGAAGCCA ACAAACTGGC AGCAAACAGC AGCATTTACA CACAGAAGAA CATGAAAGCC 
CAGGAGGAGA TGATTTCTGA ACTCAGACAG CAGAAGTTTT ATTTGGAGTC TCAGGCTGGA 
AAACTGGAGG CGCAGAATGC AAAGCTGGAG GAGCATCTGG AGAAACTCAC CCAACAAGAG 
CAGACAAACA AGAGCCGAGT GCTGGAGCTG GAAGCCAGGC TGCGAGAGAT CGGCCTAGAG 
CATGAGGAAC AGAAGCTGGA GATCAAGCGT CAGGTGTCGG AGCTCACGTT GTCTCTGCAG 
GAGAGAGAAG CTCAGATCAG CAGCCTGCAA GCGGCACGCC ACGCTCTCGA CAGCCAACTA 
CAGCAGGCCA AAACTGAGCT GGAGGAGACC ACCGCCGAGG CCGAAGAGGA GATCACCGTC 
CTGAGGGCCC ATCGGGATGA AATCCAACGC AAGTTTGATG CCCTGCGAGA CAGCTGTGCG 
GTGATCACGG ATCTGGAGGA GCAGTTGACG CAGCTCACGC AAGAAAACGC GGAGCTGAAC 
CGGCAGAACT TCTACCTGTC CAAACAGCTG GACGAGGCCA CAGACGAGAA CGAGGAGCGC 
CTGCAGCTCA AGCAGGACGT GGAGCGCCTC AGGAGAGAGG TGGCAGACCG AGAGATGCAC 
CTCAACAACC AGAAACAGGT GACACGGCAA CACACACACA CACACACACA CACACACACA 
CACAGGACAG AGGTGGCAGA CCGAGAGATG CACCTCAACA ACCAGAAACA GAACATCGAG 
ACGCTGAAGA CGACATGTAC AATGCTGGAG GAGCAGGTTA TGGAGCTGGA GACTCTGAAT 
GATGAGCTGA TGGAGAAGGA GAGGCAGTGG GAGAACTGGA GATCTGCTCT GGACGAGGAG 
AAAAACCAGG CCGAGCGCAG AGCCAGAGAC CTGCAAAGAC AGCTGGACAG CGAGAAGCAG 
AACCGACTGC GTGCAGAACA GAGAAACTCT GAGTCTCGGC AGGCCGTGGA GCTGGCGGTG 
AAAGAGCACA AAGCTGAAAT CCTGGTTCTG CAGCAGGCGC TCAAAGACCA GAAACTCAAG 
GCTGAGGGCC TTTCTGACAC TCTGGGTGAT CTGGAGAAGA AGCACGGCAT GCTGGAGATG 
AACGCTCGCA GCCTGCAGCA GAAGCTGGAG ACGGAGAGAG AGATGAAGCA GAGACTCATG 
GACGAGCAAG CTAAACTGCA GCAGCAGCTG GACATGCAGA AGAGCCACAT CTTCAGACTG 
ACTCAAGGAC TGCAGGATGC GCTGGACCAG ACCGACCTGC TGAAGACCGA GCGCACAGAC 
CTGGAGTACC AGCTGGAAAA CATACAGGCG GCGTATTCTC ACGAGAAGGT GAAGATGGAG 
GGGACGATCA CCCAGCAGAC CAAGCTCATA GACTTCCTGC AGGCCAAGGT AGAGCACCCC 
TCTAAGAAGA AAAAGGGGCT GTTTGGTGGC CGGCGGCGGG AGGATCTGCT AGCGGCGCTG 
GCGGCTCAGG GACAGACACA GGTGCCTGTG GTGCCCGCCG CCACTCCTGT GCCCCTGCAG 
TACAGCGACC TGAAGGCGGC GCTGGATAAA GAGCGAGCCC GATGCTCCGA GCTGGAGGAC 
GCCCTGCAGA AAACACGCCT GGAGCTCAGA GAAGTTCAGT TTAATAAAGC TCTGGAGCAC 
AGCTCTTCTC CAGCCACTCC AGGTTTGGCC CGTCAGCAGC TCATGATGTC CACCAAGTCC 
AACAAGTCTC CAGAGCACCA GTCCAGCGGC GGCCTGATGT CCTCAAACTC CAGCCGCGGA 
CGCAAAGACA ACGCCAATCC TGAAGAAATA AGGAGAGTCA CTTCTGAAAA GTACACCAGA 
CGTGTGAAGG ACCGGATTCA TCACAACACT CCTCACCGCT TCACGCAGGG GCTCAATATG 
AGAGCGGCTC GCTGTACTGT GTGTCTGGAC ACAGTCCATT TTGGACGTCA GGCTGCAACA 
TGCATTGAAT GTCACGCGTT GTGTCACCCA AAATGCTCCC CGTACCTCCC TGCCACCTGC 
GGCGTTCCCA CTGAATACGC GCTGCACCTC TCAGAGGCTC TGTGTCGGGA GAAGGGAAGC 
ACTTCCGGCC TGAAAGATAT GGGTGGACAT ATGAGGCTTG AAGGATGGCT GAAGCAGCCC 
AGGAACGGGA AGCGCGGGCA GGGCTGGGAG AGGAAGTATG TGGTGCTGGA TGGATATAAA 
GTGATTACAT TCGAGACAGA ACCAAGAGAC GAGTCAGTGA AGCCGCTGGA TGAGTTTGAG 
CTGTGTCTCT CTGATGGAGA AGTGACCGTT CATTCTGCTG TGGGTTCCTC TGAGCTCCCC 
AACACCGCTA AAACAGATGT GCCGTACGTG CTGAAGCTGG AGTCGTCTCC TCAGTCCACA 
TGCTGGCCCG GTCAGACTCT GTACCTCATG ACCTCGGGAT ACTCAGAGAA GCAGCGCTGG 
GTGGCAGTGC TGGAAGCCAT CGTGGCCAGC AGTCGCGGAG CCAGAGAGAA AGCTGAGGCA 
GATGCTAAAT TATTGGGTAA CTCTTTACTT AAGCTAGAAG GTGACGACCG TCTGGATATC 
AACTGCACCC TGCCGCTGAC CGACCAGATT GTGTTGGTGG GCTCTGAGGA GGGTCTCTAC 
GCACTGAATG TGATTAAGAA CTCCCTCACT CATATCCCGG GTCTGGGATC CGTGTTTCAG 
ATCCACATTA TCAAAGATCA TGACAAGCTG CTGATGATCG TGGGAGACGA GCGAGCGCTG 
TGTCTGGTGG AGATTAAGAA GGTGAAGCAG TCTTTATCCC AGTCTCACCT CCCCACACTG 
CCCGAGATTG TGCCGTATAT CTTTGAGACC GTCAAGGGCT GTCATCTGTT TGCCTCGGGC 
AGGATAGAAA ATGGCCCATG CATTTGTGCT GCGATGCCCA ATAAAGTCAC GATTCTACGA 
TACAATGACA ATCTTAACAA GTTCTGCATA CGGAAGGAGA TTGAAACTCT GGAGCCGTGC 
AGCTGCATCC ACTTCACCAG CTACAGCATC ATCATTGGTA CCAATAAATT CTACGAGATC 
GAGATGAAGC AGTGCGTTCT GGACGAGTTC CTGGACAAGA ATGACGTGTC GCTGGCGTCG 
GCTGTCTTCA CCGGATCCTC CCACAGTTTC CCCATCTCTA TCGTCCAGGT GTCCAGCACT 
CCGCAGAAGG AGGAATATCT GCTGTGTTTC CACGAGATCG GAGTTTTTGT GGATGCTTAC 
GGTCGAAGAA GTCGCCCTGA TGATCTGAAG TGGAGTCGAC TTCCCCTGAC GTTCGCCTTC 
AGAGAGCCGT ATCTCTTTGT GACCTACTTC AACTCTCTGG ATGTTATTGA GATTCAGGGA 
CACGCAGCTC TTGGGCCTCC CAGTCTGGTT CACCTGGACA TTCCTAACCC ACGTTATCTG 
GGTCCAGCCA TATCGTCTGG TGCCATCTAT CTGGCCTCGT CCTACCAGAA CAAACTGCGG 
GTGATCTGCT GTAAGGGCAG TCTGGTGCGG GAGTCTGGAG AGCTGCAGAG GACCGGCTCC 
AGCAGGAGCA GCCCCAACAA GCGCGGTCCA CCAACATACA ACGAGCACAT CTCCAAACGT 
CTGGCCTCGA GTCCTGGAGC CCAGGAGGGG CCCCTGCACC GCGAGCCCAG CACTCCTCAC 
CGCTACCGCG AGGGCCGCAC CGAGTTCCGG AGAGACAAAT CTCCAGCCCG GCCCCTGGAG 
CGAGAGAAAT CCCCGGGCCG TCCGGTGCTA GACAGCCGCA GAGAGAGGTC TCCCGGTCGC 
TTCGAGAGCA GCGGCAGCGA GAGAGACCGC GAGCGGACAC GACTGCACAC TAGCTCTGTA 
CGCACCCAGC TGACCCCCGT CAACAAGGTT TGGGACCAGT CTTCGGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212889.1
CDS	433..5403
Translation	MEQEISKVQRKMSDLESVLQQKDVELKASETQRNILEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSNKLQEIKEQEYHAQLEEMRVSIRQLEENLSAARRRSDLYESELKESRQTSEELKRKAVEYQQKIQKAKEQGKVEMEELLSKLQKTNGEQQVKIEELQEKLAKAVKASTEATELLQNVRQAKERMERDLERLQTKEDSSDSLRRRLRETEDGKKSLENQVKRLEMVERRESKLKDDIQTKSQQIQQMADKIMELEENLRDSQAMAQRMETQLEQKEKLFEDKIKVLETQMKADLAEKDLLESKQSTFEEESRERGKVISDQAATINAMESKMTSLEQRIAELSEANKLAANSSIYTQKNMKAQEEMISELRQQKFYLESQAGKLEAQNAKLEEHLEKLTQQEQTNKSRVLELEARLREIGLEHEEQKLEIKRQVSELTLSLQEREAQISSLQAARHALDSQLQQAKTELEETTAEAEEEITVLRAHRDEIQRKFDALRDSCAVITDLEEQLTQLTQENAELNRQNFYLSKQLDEATDENEERLQLKQDVERLRREVADREMHLNNQKQVTRQHTHTHTHTHTHRTEVADREMHLNNQKQNIETLKTTCTMLEEQVMELETLNDELMEKERQWENWRSALDEEKNQAERRARDLQRQLDSEKQNRLRAEQRNSESRQAVELAVKEHKAEILVLQQALKDQKLKAEGLSDTLGDLEKKHGMLEMNARSLQQKLETEREMKQRLMDEQAKLQQQLDMQKSHIFRLTQGLQDALDQTDLLKTERTDLEYQLENIQAAYSHEKVKMEGTITQQTKLIDFLQAKVEHPSKKKKGLFGGRRREDLLAALAAQGQTQVPVVPAATPVPLQYSDLKAALDKERARCSELEDALQKTRLELREVQFNKALEHSSSPATPGLARQQLMMSTKSNKSPEHQSSGGLMSSNSSRGRKDNANPEEIRRVTSEKYTRRVKDRIHHNTPHRFTQGLNMRAARCTVCLDTVHFGRQAATCIECHALCHPKCSPYLPATCGVPTEYALHLSEALCREKGSTSGLKDMGGHMRLEGWLKQPRNGKRGQGWERKYVVLDGYKVITFETEPRDESVKPLDEFELCLSDGEVTVHSAVGSSELPNTAKTDVPYVLKLESSPQSTCWPGQTLYLMTSGYSEKQRWVAVLEAIVASSRGAREKAEADAKLLGNSLLKLEGDDRLDINCTLPLTDQIVLVGSEEGLYALNVIKNSLTHIPGLGSVFQIHIIKDHDKLLMIVGDERALCLVEIKKVKQSLSQSHLPTLPEIVPYIFETVKGCHLFASGRIENGPCICAAMPNKVTILRYNDNLNKFCIRKEIETLEPCSCIHFTSYSIIIGTNKFYEIEMKQCVLDEFLDKNDVSLASAVFTGSSHSFPISIVQVSSTPQKEEYLLCFHEIGVFVDAYGRRSRPDDLKWSRLPLTFAFREPYLFVTYFNSLDVIEIQGHAALGPPSLVHLDIPNPRYLGPAISSGAIYLASSYQNKLRVICCKGSLVRESGELQRTGSSRSSPNKRGPPTYNEHISKRLASSPGAQEGPLHREPSTPHRYREGRTEFRRDKSPARPLEREKSPGRPVLDSRRERSPGRFESSGSERDRERTRLHTSSVRTQLTPVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_017357400.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357400.2

ORF Insert Sequence:

ATGGAGCAGG AGATTTCCAA AGTGCAGCGT AAGATGTCAG ACTTGGAGTC AGTTTTGCAG 
CAGAAAGACG TGGAGCTCAA GGCTTCAGAA ACCCAGAGAA ACATCTTAGA GCAAGACCTG 
GCAACCTATA TCACCGAATG CAGCAGTTTG AAGCGCAGTT TGGAGCAGGC CCGCATGGAG 
GTTTCGCAGG AAGATGATAA AGCTCTTCAG CTGCTGCACG ACATTCGAGA GCAGAGCAAC 
AAACTGCAGG AAATCAAAGA ACAGGAGTAC CACGCTCAGC TGGAGGAGAT GCGAGTGTCT 
ATTCGTCAGC TGGAAGAGAA TCTGTCGGCC GCTCGTCGCC GCAGTGACCT GTACGAGTCT 
GAGCTCAAAG AGTCCCGTCA GACCAGTGAG GAGCTCAAGA GGAAGGCAGT GGAGTATCAG 
CAGAAGATCC AGAAGGCTAA AGAACAAGGG AAAGTTGAGA TGGAGGAGCT TCTGAGCAAA 
CTGCAGAAGA CCAATGGAGA GCAACAAGTA AAGATTGAAG AGCTCCAGGA GAAGCTTGCT 
AAGGCAGTGA AGGCGAGTAC TGAAGCCACT GAACTCCTCC AGAATGTCCG TCAGGCTAAA 
GAGCGAATGG AGCGAGACCT GGAGCGTCTG CAGACCAAAG AGGACTCGTC CGACAGCCTG 
CGCAGACGCT TACGAGAGAC TGAGGATGGC AAAAAATCCC TGGAGAACCA GGTGAAGAGG 
CTGGAGATGG TGGAGCGGAG AGAATCCAAA CTGAAAGACG ACATTCAGAC CAAATCGCAG 
CAGATCCAGC AGATGGCCGA CAAAATCATG GAGCTTGAAG AAAATTTGCG GGACTCGCAG 
GCGATGGCGC AGCGCATGGA AACACAGCTG GAGCAGAAGG AAAAGCTTTT TGAAGACAAG 
ATCAAGGTTT TAGAAACTCA AATGAAGGCT GATTTGGCTG AGAAGGATTT GCTGGAGTCC 
AAACAGAGCA CTTTTGAAGA GGAGTCTCGC GAACGGGGAA AAGTGATCAG CGACCAGGCA 
GCGACGATCA ATGCCATGGA GTCAAAGATG ACCAGTCTGG AGCAGAGGAT CGCTGAGCTG 
TCCGAAGCCA ACAAACTGGC AGCAAACAGC AGCATTTACA CACAGAAGAA CATGAAAGCC 
CAGGAGGAGA TGATTTCTGA ACTCAGACAG CAGAAGTTTT ATTTGGAGTC TCAGGCTGGA 
AAACTGGAGG CGCAGAATGC AAAGCTGGAG GAGCATCTGG AGAAACTCAC CCAACAAGAG 
CAGACAAACA AGAGCCGAGT GCTGGAGCTG GAAGCCAGGC TGCGAGAGAT CGGCCTAGAG 
CATGAGGAAC AGAAGCTGGA GATCAAGCGT CAGGTGTCGG AGCTCACGTT GTCTCTGCAG 
GAGAGAGAAG CTCAGATCAG CAGCCTGCAA GCGGCACGCC ACGCTCTCGA CAGCCAACTA 
CAGCAGGCCA AAACTGAGCT GGAGGAGACC ACCGCCGAGG CCGAAGAGGA GATCACCGTC 
CTGAGGGCCC ATCGGGATGA AATCCAACGC AAGTTTGATG CCCTGCGAGA CAGCTGTGCG 
GTGATCACGG ATCTGGAGGA GCAGTTGACG CAGCTCACGC AAGAAAACGC GGAGCTGAAC 
CGGCAGAACT TCTACCTGTC CAAACAGCTG GACGAGGCCA CAGACGAGAA CGAGGAGCGC 
CTGCAGCTCA AGCAGGACGT GGAGCGCCTC AGGAGAGAGG TGGCAGACCG AGAGATGCAC 
CTCAACAACC AGAAACAGGT GACACGGCAA CACACACACA CACACACACA CACACACACA 
CACAGGACAG AGGTGGCAGA CCGAGAGATG CACCTCAACA ACCAGAAACA GAACATCGAG 
ACGCTGAAGA CGACATGTAC AATGCTGGAG GAGCAGGTTA TGGAGCTGGA GACTCTGAAT 
GATGAGCTGA TGGAGAAGGA GAGGCAGTGG GAGAACTGGA GATCTGCTCT GGACGAGGAG 
AAAAACCAGG CCGAGCGCAG AGCCAGAGAC CTGCAAAGAC AGCTGGACAG CGAGAAGCAG 
AACCGACTGC GTGCAGAACA GAGAAACTCT GAGTCTCGGC AGGCCGTGGA GCTGGCGGTG 
AAAGAGCACA AAGCTGAAAT CCTGGTTCTG CAGCAGGCGC TCAAAGACCA GAAACTCAAG 
GCTGAGGGCC TTTCTGACAC TCTGGGTGAT CTGGAGAAGA AGCACGGCAT GCTGGAGATG 
AACGCTCGCA GCCTGCAGCA GAAGCTGGAG ACGGAGAGAG AGATGAAGCA GAGACTCATG 
GACGAGCAAG CTAAACTGCA GCAGCAGCTG GACATGCAGA AGAGCCACAT CTTCAGACTG 
ACTCAAGGAC TGCAGGATGC GCTGGACCAG ACCGACCTGC TGAAGACCGA GCGCACAGAC 
CTGGAGTACC AGCTGGAAAA CATACAGGCG GCGTATTCTC ACGAGAAGGT GAAGATGGAG 
GGGACGATCA CCCAGCAGAC CAAGCTCATA GACTTCCTGC AGGCCAAGGT AGAGCACCCC 
TCTAAGAAGA AAAAGGGGCT GTTTGGTGGC CGGCGGCGGG AGGATCTGCT AGCGGCGCTG 
GCGGCTCAGG GACAGACACA GGTGCCTGTG GTGCCCGCCG CCACTCCTGT GCCCCTGCAG 
TACAGCGACC TGAAGGCGGC GCTGGATAAA GAGCGAGCCC GATGCTCCGA GCTGGAGGAC 
GCCCTGCAGA AAACACGCCT GGAGCTCAGA GAAGTTCAGT TTAATAAAGC TCTGGAGCAC 
AGCTCTTCTC CAGCCACTCC AGGTTTGGCC CGTCAGCAGC TCATGATGTC CACCAAGTCC 
AACAAGTCTC CAGAGCACCA GTCCAGCGGC GGCCTGATGT CCTCAAACTC CAGCCGCGGA 
CGCAAAGACA ACGCCAATCC TGAAGAAATA AGGAGAGTCA CTTCTGAAAA GTACACCAGA 
CGTGTGAAGG ACCGGATTCA TCACAACACT CCTCACCGCT TCACGCAGGG GCTCAATATG 
AGAGCGGCTC GCTGTACTGT GTGTCTGGAC ACAGTCCATT TTGGACGTCA GGCTGCAACA 
TGCATTGAAT GTCACGCGTT GTGTCACCCA AAATGCTCCC CGTACCTCCC TGCCACCTGC 
GGCGTTCCCA CTGAATACGC GCTGCACCTC TCAGAGGCTC TGTGTCGGGA GAAGGGAAGC 
ACTTCCGGCC TGAAAGATAT GGGTGGACAT ATGAGGCTTG AAGGATGGCT GAAGCAGCCC 
AGGAACGGGA AGCGCGGGCA GGGCTGGGAG AGGAAGTATG TGGTGCTGGA TGGATATAAA 
GTGATTACAT TCGAGACAGA ACCAAGAGAC GAGTCAGTGA AGCCGCTGGA TGAGTTTGAG 
CTGTGTCTCT CTGATGGAGA AGTGACCGTT CATTCTGCTG TGGGTTCCTC TGAGCTCCCC 
AACACCGCTA AAACAGATGT GCCGTACGTG CTGAAGCTGG AGTCGTCTCC TCAGTCCACA 
TGCTGGCCCG GTCAGACTCT GTACCTCATG ACCTCGGGAT ACTCAGAGAA GCAGCGCTGG 
GTGGCAGTGC TGGAAGCCAT CGTGGCCAGC AGTCGCGGAG CCAGAGAGAA AGCTGAGGCA 
GATGCTAAAT TATTGGGTAA CTCTTTACTT AAGCTAGAAG GTGACGACCG TCTGGATATC 
AACTGCACCC TGCCGCTGAC CGACCAGATT GTGTTGGTGG GCTCTGAGGA GGGTCTCTAC 
GCACTGAATG TGATTAAGAA CTCCCTCACT CATATCCCGG GTCTGGGATC CGTGTTTCAG 
ATCCACATTA TCAAAGATCA TGACAAGCTG CTGATGATCG TGGGAGACGA GCGAGCGCTG 
TGTCTGGTGG AGATTAAGAA GGTGAAGCAG TCTTTATCCC AGTCTCACCT CCCCACACTG 
CCCGAGATTG TGCCGTATAT CTTTGAGACC GTCAAGGGCT GTCATCTGTT TGCCTCGGGC 
AGGATAGAAA ATGGCCCATG CATTTGTGCT GCGATGCCCA ATAAAGTCAC GATTCTACGA 
TACAATGACA ATCTTAACAA GTTCTGCATA CGGAAGGAGA TTGAAACTCT GGAGCCGTGC 
AGCTGCATCC ACTTCACCAG CTACAGCATC ATCATTGGTA CCAATAAATT CTACGAGATC 
GAGATGAAGC AGTGCGTTCT GGACGAGTTC CTGGACAAGA ATGACGTGTC GCTGGCGTCG 
GCTGTCTTCA CCGGATCCTC CCACAGTTTC CCCATCTCTA TCGTCCAGGT GTCCAGCACT 
CCGCAGAAGG AGGAATATCT GCTGTGTTTC CACGAGATCG GAGTTTTTGT GGATGCTTAC 
GGTCGAAGAA GTCGCCCTGA TGATCTGAAG TGGAGTCGAC TTCCCCTGAC GTTCGCCTTC 
AGAGAGCCGT ATCTCTTTGT GACCTACTTC AACTCTCTGG ATGTTATTGA GATTCAGGGA 
CACGCAGCTC TTGGGCCTCC CAGTCTGGTT CACCTGGACA TTCCTAACCC ACGTTATCTG 
GGTCCAGCCA TATCGTCTGG TGCCATCTAT CTGGCCTCGT CCTACCAGAA CAAACTGCGG 
GTGATCTGCT GTAAGGGCAG TCTGGTGCGG GAGTCTGGAG AGCTGCAGAG GACCGGCTCC 
AGCAGGAGCA GCCCCAACAA GCGCGGTCCA CCAACATACA ACGAGCACAT CTCCAAACGT 
CTGGCCTCGA GTCCTGGAGC CCAGGAGGGG CCCCTGCACC GCGAGCCCAG CACTCCTCAC 
CGCTACCGCG AGGGCCGCAC CGAGTTCCGG AGAGACAAAT CTCCAGCCCG GCCCCTGGAG 
CGAGAGAAAT CCCCGGGCCG TCCGGTGCTA GACAGCCGCA GAGAGAGGTC TCCCGGTCGC 
TTCGAGAGCA GCGGCAGCGA GAGAGACCGC GAGCGGACAC GACTGCACAC TAGCTCTGTA 
CGCACCCAGC TGACCCCCGT CAACAAGGTT TGGGACCAGT CTTCGGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100333880 ( XM_017357402.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212891.1 Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X3, mRNA.

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CloneID	ODa60006
Clone ID Related Accession (Same CDS sequence)	XM_017357402.1
Accession Version	XM_017357402.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	citron Rho-interacting kinase-like isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG AGATTTCCAA AGTGCAGCGT AAGATGTCAG ACTTGGAGTC AGTTTTGCAG 
CAGAAAGACG TGGAGCTCAA GGCTTCAGAA ACCCAGAGAA ACATCTTAGA GCAAGACCTG 
GCAACCTATA TCACCGAATG CAGCAGTTTG AAGCGCAGTT TGGAGCAGGC CCGCATGGAG 
GTTTCGCAGG AAGATGATAA AGCTCTTCAG CTGCTGCACG ACATTCGAGA GCAGAGCAAC 
AAACTGCAGG AAATCAAAGA ACAGGAGTAC CACGCTCAGC TGGAGGAGAT GCGAGTGTCT 
ATTCGTCAGC TGGAAGAGAA TCTGTCGGCC GCTCGTCGCC GCAGTGACCT GTACGAGTCT 
GAGCTCAAAG AGTCCCGTCA GACCAGTGAG GAGCTCAAGA GGAAGGCAGT GGAGTATCAG 
CAGAAGATCC AGAAGGCTAA AGAACAAGGG AAAGTTGAGA TGGAGGAGCT TCTGAGCAAA 
CTGCAGAAGA CCAATGGAGA GCAACAAGTA AAGATTGAAG AGCTCCAGGA GAAGCTTGCT 
AAGGCAGTGA AGGCGAGTAC TGAAGCCACT GAACTCCTCC AGAATGTCCG TCAGGCTAAA 
GAGCGAATGG AGCGAGACCT GGAGCGTCTG CAGACCAAAG AGGACTCGTC CGACAGCCTG 
CGCAGACGCT TACGAGAGAC TGAGGATGGC AAAAAATCCC TGGAGAACCA GGTGAAGAGG 
CTGGAGATGG TGGAGCGGAG AGAATCCAAA CTGAAAGACG ACATTCAGAC CAAATCGCAG 
CAGATCCAGC AGATGGCCGA CAAAATCATG GAGCTTGAAG AAAATTTGCG GGACTCGCAG 
GCGATGGCGC AGCGCATGGA AACACAGCTG GAGCAGAAGG AAAAGCTTTT TGAAGACAAG 
ATCAAGGTTT TAGAAACTCA AATGAAGGCT GATTTGGCTG AGAAGGATTT GCTGGAGTCC 
AAACAGAGCA CTTTTGAAGA GGAGTCTCGC GAACGGGGAA AAGTGATCAG CGACCAGGCA 
GCGACGATCA ATGCCATGGA GTCAAAGATG ACCAGTCTGG AGCAGAGGAT CGCTGAGCTG 
TCCGAAGCCA ACAAACTGGC AGCAAACAGC AGCATTTACA CACAGAAGAA CATGAAAGCC 
CAGGAGGAGA TGATTTCTGA ACTCAGACAG CAGAAGTTTT ATTTGGAGTC TCAGGCTGGA 
AAACTGGAGG CGCAGAATGC AAAGCTGGAG GAGCATCTGG AGAAACTCAC CCAACAAGAG 
CAGACAAACA AGAGCCGAGT GCTGGAGCTG GAAGCCAGGC TGCGAGAGAT CGGCCTAGAG 
CATGAGGAAC AGAAGCTGGA GATCAAGCGT CAGGTGTCGG AGCTCACGTT GTCTCTGCAG 
GAGAGAGAAG CTCAGATCAG CAGCCTGCAA GCGGCACGCC ACGCTCTCGA CAGCCAACTA 
CAGCAGGCCA AAACTGAGCT GGAGGAGACC ACCGCCGAGG CCGAAGAGGA GATCACCGTC 
CTGAGGGCCC ATCGGGATGA AATCCAACGC AAGTTTGATG CCCTGCGAGA CAGCTGTGCG 
GTGATCACGG ATCTGGAGGA GCAGTTGACG CAGCTCACGC AAGAAAACGC GGAGCTGAAC 
CGGCAGAACT TCTACCTGTC CAAACAGCTG GACGAGGCCA CAGACGAGAA CGAGGAGCGC 
CTGCAGCTCA AGCAGGACGT GGAGCGCCTC AGGAGAGAGG TGGCAGACCG AGAGATGCAC 
CTCAACAACC AGAAACAGGT GACACGGCAA CACACACACA CACACACACA CACACACACA 
CACAGGACAG AGGTGGCAGA CCGAGAGATG CACCTCAACA ACCAGAAACA GAACATCGAG 
ACGCTGAAGA CGACATGTAC AATGCTGGAG GAGCAGGTTA TGGAGCTGGA GACTCTGAAT 
GATGAGCTGA TGGAGAAGGA GAGGCAGTGG GAGAACTGGA GATCTGCTCT GGACGAGGAG 
AAAAACCAGG CCGAGCGCAG AGCCAGAGAC CTGCAAAGAC AGCTGGACAG CGAGAAGCAG 
AACCGACTGC GTGCAGAACA GAGAAACTCT GAGTCTCGGC AGGCCGTGGA GCTGGCGGTG 
AAAGAGCACA AAGCTGAAAT CCTGGTTCTG CAGCAGGCGC TCAAAGACCA GAAACTCAAG 
GCTGAGGGCC TTTCTGACAC TCTGGGTGAT CTGGAGAAGA AGCACGGCAT GCTGGAGATG 
AACGCTCGCA GCCTGCAGCA GAAGCTGGAG ACGGAGAGAG AGATGAAGCA GAGACTCATG 
GACGAGCAAG CTAAACTGCA GCAGCAGCTG GACATGCAGA AGAGCCACAT CTTCAGACTG 
ACTCAAGGAC TGCAGGATGC GCTGGACCAG ACCGACCTGC TGAAGACCGA GCGCACAGAC 
CTGGAGTACC AGCTGGAAAA CATACAGGCG GCGTATTCTC ACGAGAAGGT GAAGATGGAG 
GGGACGATCA CCCAGCAGAC CAAGCTCATA GACTTCCTGC AGGCCAAGGT AGAGCACCCC 
TCTAAGAAGA AAAAGGGGCT GTTTGGTGGC CGGCGGCGGG AGGATCTGCT AGCGGCGCTG 
GCGGCTCAGG GACAGACACA GGTGCCTGTG GTGCCCGCCG CCACTCCTGT GCCCCTGCAG 
TACAGCGACC TGAAGGCGGC GCTGGATAAA GAGCGAGCCC GATGCTCCGA GCTGGAGGAC 
GCCCTGCAGA AAACACGCCT GGAGCTCAGA GAAGTTCAGT TTAATAAAGC TCTGGAGCAC 
AGCTCTTCTC CAGCCACTCC AGGTTTGGCC CGTCAGCAGC TCATGATGTC CACCAAGTCC 
AACAAGTCTC CAGAGCACCA GTCCAGCGGC GGCCTGATGT CCTCAAACTC CAGCCGCGGA 
CGCAAAGACA ACGCCAATCC TGAAGAAATA AGGAGAGTCA CTTCTGAAAA GTACACCAGA 
CGTGTGAAGG ACCGGATTCA TCACAACACT CCTCACCGCT TCACGCAGGG GCTCAATATG 
AGAGCGGCTC GCTGTACTGT GTGTCTGGAC ACAGTCCATT TTGGACGTCA GGCTGCAACA 
TGCATTGAAT GTCACGCGTT GTGTCACCCA AAATGCTCCC CGTACCTCCC TGCCACCTGC 
GGCGTTCCCA CTGAATACGC GCTGCACCTC TCAGAGGCTC TGTGTCGGGA GAAGGGAAGC 
ACTTCCGGCC TGAAAGATAT GGGTGGACAT ATGAGGCTTG AAGGATGGCT GAAGCAGCCC 
AGGAACGGGA AGCGCGGGCA GGGCTGGGAG AGGAAGTATG TGGTGCTGGA TGGATATAAA 
GTGATTACAT TCGAGACAGA ACCAAGAGAC GAGTCAGTGA AGCCGCTGGA TGAGTTTGAG 
CTGTGTCTCT CTGATGGAGA AGTGACCGTT CATTCTGCTG TGGGTTCCTC TGAGCTCCCC 
AACACCGCTA AAACAGATGT GCCGTACGTG CTGAAGCTGG AGTCGTCTCC TCAGTCCACA 
TGCTGGCCCG GTCAGACTCT GTACCTCATG ACCTCGGGAT ACTCAGAGAA GCAGCGCTGG 
GTGGCAGTGC TGGAAGCCAT CGTGGCCAGC AGTCGCGGAG CCAGAGAGAA AGCTGAGGCA 
GATGCTAAAT TATTGGGTAA CTCTTTACTT AAGCTAGAAG GTGACGACCG TCTGGATATC 
AACTGCACCC TGCCGCTGAC CGACCAGATT GTGTTGGTGG GCTCTGAGGA GGGTCTCTAC 
GCACTGAATG TGATTAAGAA CTCCCTCACT CATATCCCGG GTCTGGGATC CGTGTTTCAG 
ATCCACATTA TCAAAGATCA TGACAAGCTG CTGATGATCG TGGGAGACGA GCGAGCGCTG 
TGTCTGGTGG AGATTAAGAA GGTGAAGCAG TCTTTATCCC AGTCTCACCT CCCCACACTG 
CCCGAGATTG TGCCGTATAT CTTTGAGACC GTCAAGGGCT GTCATCTGTT TGCCTCGGGC 
AGGATAGAAA ATGGCCCATG CATTTGTGCT GCGATGCCCA ATAAAGTCAC GATTCTACGA 
TACAATGACA ATCTTAACAA GTTCTGCATA CGGAAGGAGA TTGAAACTCT GGAGCCGTGC 
AGCTGCATCC ACTTCACCAG CTACAGCATC ATCATTGGTA CCAATAAATT CTACGAGATC 
GAGATGAAGC AGTGCGTTCT GGACGAGTTC CTGGACAAGA ATGACGTGTC GCTGGCGTCG 
GCTGTCTTCA CCGGATCCTC CCACAGTTTC CCCATCTCTA TCGTCCAGGT GTCCAGCACT 
CCGCAGAAGG AGGAATATCT GCTGTGTTTC CACGAGATCG GAGTTTTTGT GGATGCTTAC 
GGTCGAAGAA GTCGCCCTGA TGATCTGAAG TGGAGTCGAC TTCCCCTGAC GTTCGCCTTC 
AGAGAGCCGT ATCTCTTTGT GACCTACTTC AACTCTCTGG ATGTTATTGA GATTCAGGGA 
CACGCAGCTC TTGGGCCTCC CAGTCTGGTT CACCTGGACA TTCCTAACCC ACGTTATCTG 
GGTCCAGCCA TATCGTCTGG TGCCATCTAT CTGGCCTCGT CCTACCAGAA CAAACTGCGG 
GTGATCTGCT GTAAGGGCAG TCTGGTGCGG GAGTCTGGAG AGCTGCAGAG GACCGGCTCC 
AGCAGGAGCC CCAACAAGCG CGGTCCACCA ACATACAACG AGCACATCTC CAAACGTCTG 
GCCTCGAGTC CTGGAGCCCA GGAGGGGCCC CTGCACCGCG AGCCCAGCAC TCCTCACCGC 
TACCGCGAGG GCCGCACCGA GTTCCGGAGA GACAAATCTC CAGCCCGGCC CCTGGAGCGA 
GAGAAATCCC CGGGCCGTCC GGTGCTAGAC AGCCGCAGAG AGAGGTCTCC CGGTCGCTTC 
GAGAGCAGCG GCAGCGAGAG AGACCGCGAG CGGACACGAC TGCACACTAG CTCTGTACGC 
ACCCAGCTGA CCCCCGTCAA CAAGGTTTGG GACCAGTCTT CGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212891.1
CDS	434..5401
Translation	MEQEISKVQRKMSDLESVLQQKDVELKASETQRNILEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSNKLQEIKEQEYHAQLEEMRVSIRQLEENLSAARRRSDLYESELKESRQTSEELKRKAVEYQQKIQKAKEQGKVEMEELLSKLQKTNGEQQVKIEELQEKLAKAVKASTEATELLQNVRQAKERMERDLERLQTKEDSSDSLRRRLRETEDGKKSLENQVKRLEMVERRESKLKDDIQTKSQQIQQMADKIMELEENLRDSQAMAQRMETQLEQKEKLFEDKIKVLETQMKADLAEKDLLESKQSTFEEESRERGKVISDQAATINAMESKMTSLEQRIAELSEANKLAANSSIYTQKNMKAQEEMISELRQQKFYLESQAGKLEAQNAKLEEHLEKLTQQEQTNKSRVLELEARLREIGLEHEEQKLEIKRQVSELTLSLQEREAQISSLQAARHALDSQLQQAKTELEETTAEAEEEITVLRAHRDEIQRKFDALRDSCAVITDLEEQLTQLTQENAELNRQNFYLSKQLDEATDENEERLQLKQDVERLRREVADREMHLNNQKQVTRQHTHTHTHTHTHRTEVADREMHLNNQKQNIETLKTTCTMLEEQVMELETLNDELMEKERQWENWRSALDEEKNQAERRARDLQRQLDSEKQNRLRAEQRNSESRQAVELAVKEHKAEILVLQQALKDQKLKAEGLSDTLGDLEKKHGMLEMNARSLQQKLETEREMKQRLMDEQAKLQQQLDMQKSHIFRLTQGLQDALDQTDLLKTERTDLEYQLENIQAAYSHEKVKMEGTITQQTKLIDFLQAKVEHPSKKKKGLFGGRRREDLLAALAAQGQTQVPVVPAATPVPLQYSDLKAALDKERARCSELEDALQKTRLELREVQFNKALEHSSSPATPGLARQQLMMSTKSNKSPEHQSSGGLMSSNSSRGRKDNANPEEIRRVTSEKYTRRVKDRIHHNTPHRFTQGLNMRAARCTVCLDTVHFGRQAATCIECHALCHPKCSPYLPATCGVPTEYALHLSEALCREKGSTSGLKDMGGHMRLEGWLKQPRNGKRGQGWERKYVVLDGYKVITFETEPRDESVKPLDEFELCLSDGEVTVHSAVGSSELPNTAKTDVPYVLKLESSPQSTCWPGQTLYLMTSGYSEKQRWVAVLEAIVASSRGAREKAEADAKLLGNSLLKLEGDDRLDINCTLPLTDQIVLVGSEEGLYALNVIKNSLTHIPGLGSVFQIHIIKDHDKLLMIVGDERALCLVEIKKVKQSLSQSHLPTLPEIVPYIFETVKGCHLFASGRIENGPCICAAMPNKVTILRYNDNLNKFCIRKEIETLEPCSCIHFTSYSIIIGTNKFYEIEMKQCVLDEFLDKNDVSLASAVFTGSSHSFPISIVQVSSTPQKEEYLLCFHEIGVFVDAYGRRSRPDDLKWSRLPLTFAFREPYLFVTYFNSLDVIEIQGHAALGPPSLVHLDIPNPRYLGPAISSGAIYLASSYQNKLRVICCKGSLVRESGELQRTGSSRSPNKRGPPTYNEHISKRLASSPGAQEGPLHREPSTPHRYREGRTEFRRDKSPARPLEREKSPGRPVLDSRRERSPGRFESSGSERDRERTRLHTSSVRTQLTPVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_017357402.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357402.1

ORF Insert Sequence:

ATGGAGCAGG AGATTTCCAA AGTGCAGCGT AAGATGTCAG ACTTGGAGTC AGTTTTGCAG 
CAGAAAGACG TGGAGCTCAA GGCTTCAGAA ACCCAGAGAA ACATCTTAGA GCAAGACCTG 
GCAACCTATA TCACCGAATG CAGCAGTTTG AAGCGCAGTT TGGAGCAGGC CCGCATGGAG 
GTTTCGCAGG AAGATGATAA AGCTCTTCAG CTGCTGCACG ACATTCGAGA GCAGAGCAAC 
AAACTGCAGG AAATCAAAGA ACAGGAGTAC CACGCTCAGC TGGAGGAGAT GCGAGTGTCT 
ATTCGTCAGC TGGAAGAGAA TCTGTCGGCC GCTCGTCGCC GCAGTGACCT GTACGAGTCT 
GAGCTCAAAG AGTCCCGTCA GACCAGTGAG GAGCTCAAGA GGAAGGCAGT GGAGTATCAG 
CAGAAGATCC AGAAGGCTAA AGAACAAGGG AAAGTTGAGA TGGAGGAGCT TCTGAGCAAA 
CTGCAGAAGA CCAATGGAGA GCAACAAGTA AAGATTGAAG AGCTCCAGGA GAAGCTTGCT 
AAGGCAGTGA AGGCGAGTAC TGAAGCCACT GAACTCCTCC AGAATGTCCG TCAGGCTAAA 
GAGCGAATGG AGCGAGACCT GGAGCGTCTG CAGACCAAAG AGGACTCGTC CGACAGCCTG 
CGCAGACGCT TACGAGAGAC TGAGGATGGC AAAAAATCCC TGGAGAACCA GGTGAAGAGG 
CTGGAGATGG TGGAGCGGAG AGAATCCAAA CTGAAAGACG ACATTCAGAC CAAATCGCAG 
CAGATCCAGC AGATGGCCGA CAAAATCATG GAGCTTGAAG AAAATTTGCG GGACTCGCAG 
GCGATGGCGC AGCGCATGGA AACACAGCTG GAGCAGAAGG AAAAGCTTTT TGAAGACAAG 
ATCAAGGTTT TAGAAACTCA AATGAAGGCT GATTTGGCTG AGAAGGATTT GCTGGAGTCC 
AAACAGAGCA CTTTTGAAGA GGAGTCTCGC GAACGGGGAA AAGTGATCAG CGACCAGGCA 
GCGACGATCA ATGCCATGGA GTCAAAGATG ACCAGTCTGG AGCAGAGGAT CGCTGAGCTG 
TCCGAAGCCA ACAAACTGGC AGCAAACAGC AGCATTTACA CACAGAAGAA CATGAAAGCC 
CAGGAGGAGA TGATTTCTGA ACTCAGACAG CAGAAGTTTT ATTTGGAGTC TCAGGCTGGA 
AAACTGGAGG CGCAGAATGC AAAGCTGGAG GAGCATCTGG AGAAACTCAC CCAACAAGAG 
CAGACAAACA AGAGCCGAGT GCTGGAGCTG GAAGCCAGGC TGCGAGAGAT CGGCCTAGAG 
CATGAGGAAC AGAAGCTGGA GATCAAGCGT CAGGTGTCGG AGCTCACGTT GTCTCTGCAG 
GAGAGAGAAG CTCAGATCAG CAGCCTGCAA GCGGCACGCC ACGCTCTCGA CAGCCAACTA 
CAGCAGGCCA AAACTGAGCT GGAGGAGACC ACCGCCGAGG CCGAAGAGGA GATCACCGTC 
CTGAGGGCCC ATCGGGATGA AATCCAACGC AAGTTTGATG CCCTGCGAGA CAGCTGTGCG 
GTGATCACGG ATCTGGAGGA GCAGTTGACG CAGCTCACGC AAGAAAACGC GGAGCTGAAC 
CGGCAGAACT TCTACCTGTC CAAACAGCTG GACGAGGCCA CAGACGAGAA CGAGGAGCGC 
CTGCAGCTCA AGCAGGACGT GGAGCGCCTC AGGAGAGAGG TGGCAGACCG AGAGATGCAC 
CTCAACAACC AGAAACAGGT GACACGGCAA CACACACACA CACACACACA CACACACACA 
CACAGGACAG AGGTGGCAGA CCGAGAGATG CACCTCAACA ACCAGAAACA GAACATCGAG 
ACGCTGAAGA CGACATGTAC AATGCTGGAG GAGCAGGTTA TGGAGCTGGA GACTCTGAAT 
GATGAGCTGA TGGAGAAGGA GAGGCAGTGG GAGAACTGGA GATCTGCTCT GGACGAGGAG 
AAAAACCAGG CCGAGCGCAG AGCCAGAGAC CTGCAAAGAC AGCTGGACAG CGAGAAGCAG 
AACCGACTGC GTGCAGAACA GAGAAACTCT GAGTCTCGGC AGGCCGTGGA GCTGGCGGTG 
AAAGAGCACA AAGCTGAAAT CCTGGTTCTG CAGCAGGCGC TCAAAGACCA GAAACTCAAG 
GCTGAGGGCC TTTCTGACAC TCTGGGTGAT CTGGAGAAGA AGCACGGCAT GCTGGAGATG 
AACGCTCGCA GCCTGCAGCA GAAGCTGGAG ACGGAGAGAG AGATGAAGCA GAGACTCATG 
GACGAGCAAG CTAAACTGCA GCAGCAGCTG GACATGCAGA AGAGCCACAT CTTCAGACTG 
ACTCAAGGAC TGCAGGATGC GCTGGACCAG ACCGACCTGC TGAAGACCGA GCGCACAGAC 
CTGGAGTACC AGCTGGAAAA CATACAGGCG GCGTATTCTC ACGAGAAGGT GAAGATGGAG 
GGGACGATCA CCCAGCAGAC CAAGCTCATA GACTTCCTGC AGGCCAAGGT AGAGCACCCC 
TCTAAGAAGA AAAAGGGGCT GTTTGGTGGC CGGCGGCGGG AGGATCTGCT AGCGGCGCTG 
GCGGCTCAGG GACAGACACA GGTGCCTGTG GTGCCCGCCG CCACTCCTGT GCCCCTGCAG 
TACAGCGACC TGAAGGCGGC GCTGGATAAA GAGCGAGCCC GATGCTCCGA GCTGGAGGAC 
GCCCTGCAGA AAACACGCCT GGAGCTCAGA GAAGTTCAGT TTAATAAAGC TCTGGAGCAC 
AGCTCTTCTC CAGCCACTCC AGGTTTGGCC CGTCAGCAGC TCATGATGTC CACCAAGTCC 
AACAAGTCTC CAGAGCACCA GTCCAGCGGC GGCCTGATGT CCTCAAACTC CAGCCGCGGA 
CGCAAAGACA ACGCCAATCC TGAAGAAATA AGGAGAGTCA CTTCTGAAAA GTACACCAGA 
CGTGTGAAGG ACCGGATTCA TCACAACACT CCTCACCGCT TCACGCAGGG GCTCAATATG 
AGAGCGGCTC GCTGTACTGT GTGTCTGGAC ACAGTCCATT TTGGACGTCA GGCTGCAACA 
TGCATTGAAT GTCACGCGTT GTGTCACCCA AAATGCTCCC CGTACCTCCC TGCCACCTGC 
GGCGTTCCCA CTGAATACGC GCTGCACCTC TCAGAGGCTC TGTGTCGGGA GAAGGGAAGC 
ACTTCCGGCC TGAAAGATAT GGGTGGACAT ATGAGGCTTG AAGGATGGCT GAAGCAGCCC 
AGGAACGGGA AGCGCGGGCA GGGCTGGGAG AGGAAGTATG TGGTGCTGGA TGGATATAAA 
GTGATTACAT TCGAGACAGA ACCAAGAGAC GAGTCAGTGA AGCCGCTGGA TGAGTTTGAG 
CTGTGTCTCT CTGATGGAGA AGTGACCGTT CATTCTGCTG TGGGTTCCTC TGAGCTCCCC 
AACACCGCTA AAACAGATGT GCCGTACGTG CTGAAGCTGG AGTCGTCTCC TCAGTCCACA 
TGCTGGCCCG GTCAGACTCT GTACCTCATG ACCTCGGGAT ACTCAGAGAA GCAGCGCTGG 
GTGGCAGTGC TGGAAGCCAT CGTGGCCAGC AGTCGCGGAG CCAGAGAGAA AGCTGAGGCA 
GATGCTAAAT TATTGGGTAA CTCTTTACTT AAGCTAGAAG GTGACGACCG TCTGGATATC 
AACTGCACCC TGCCGCTGAC CGACCAGATT GTGTTGGTGG GCTCTGAGGA GGGTCTCTAC 
GCACTGAATG TGATTAAGAA CTCCCTCACT CATATCCCGG GTCTGGGATC CGTGTTTCAG 
ATCCACATTA TCAAAGATCA TGACAAGCTG CTGATGATCG TGGGAGACGA GCGAGCGCTG 
TGTCTGGTGG AGATTAAGAA GGTGAAGCAG TCTTTATCCC AGTCTCACCT CCCCACACTG 
CCCGAGATTG TGCCGTATAT CTTTGAGACC GTCAAGGGCT GTCATCTGTT TGCCTCGGGC 
AGGATAGAAA ATGGCCCATG CATTTGTGCT GCGATGCCCA ATAAAGTCAC GATTCTACGA 
TACAATGACA ATCTTAACAA GTTCTGCATA CGGAAGGAGA TTGAAACTCT GGAGCCGTGC 
AGCTGCATCC ACTTCACCAG CTACAGCATC ATCATTGGTA CCAATAAATT CTACGAGATC 
GAGATGAAGC AGTGCGTTCT GGACGAGTTC CTGGACAAGA ATGACGTGTC GCTGGCGTCG 
GCTGTCTTCA CCGGATCCTC CCACAGTTTC CCCATCTCTA TCGTCCAGGT GTCCAGCACT 
CCGCAGAAGG AGGAATATCT GCTGTGTTTC CACGAGATCG GAGTTTTTGT GGATGCTTAC 
GGTCGAAGAA GTCGCCCTGA TGATCTGAAG TGGAGTCGAC TTCCCCTGAC GTTCGCCTTC 
AGAGAGCCGT ATCTCTTTGT GACCTACTTC AACTCTCTGG ATGTTATTGA GATTCAGGGA 
CACGCAGCTC TTGGGCCTCC CAGTCTGGTT CACCTGGACA TTCCTAACCC ACGTTATCTG 
GGTCCAGCCA TATCGTCTGG TGCCATCTAT CTGGCCTCGT CCTACCAGAA CAAACTGCGG 
GTGATCTGCT GTAAGGGCAG TCTGGTGCGG GAGTCTGGAG AGCTGCAGAG GACCGGCTCC 
AGCAGGAGCC CCAACAAGCG CGGTCCACCA ACATACAACG AGCACATCTC CAAACGTCTG 
GCCTCGAGTC CTGGAGCCCA GGAGGGGCCC CTGCACCGCG AGCCCAGCAC TCCTCACCGC 
TACCGCGAGG GCCGCACCGA GTTCCGGAGA GACAAATCTC CAGCCCGGCC CCTGGAGCGA 
GAGAAATCCC CGGGCCGTCC GGTGCTAGAC AGCCGCAGAG AGAGGTCTCC CGGTCGCTTC 
GAGAGCAGCG GCAGCGAGAG AGACCGCGAG CGGACACGAC TGCACACTAG CTCTGTACGC 
ACCCAGCTGA CCCCCGTCAA CAAGGTTTGG GACCAGTCTT CGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100333880 ( XM_009303720.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301995.2 Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X6, mRNA.

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CloneID	ODa60007
Clone ID Related Accession (Same CDS sequence)	XM_009303720.3 , XM_009303720.2
Accession Version	XM_009303720.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	citron Rho-interacting kinase-like isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303720.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG AGATTTCCAA AGTGCAGCGT AAGATGTCAG ACTTGGAGTC AGTTTTGCAG 
CAGAAAGACG TGGAGCTCAA GGCTTCAGAA ACCCAGAGAA ACATCTTAGA GCAAGACCTG 
GCAACCTATA TCACCGAATG CAGCAGTTTG AAGCGCAGTT TGGAGCAGGC CCGCATGGAG 
GTTTCGCAGG AAGATGATAA AGCTCTTCAG CTGCTGCACG ACATTCGAGA GCAGAGCAAC 
AAACTGCAGG AAATCAAAGA ACAGGAGTAC CACGCTCAGC TGGAGGAGAT GCGAGTGTCT 
ATTCGTCAGC TGGAAGAGAA TCTGTCGGCC GCTCGTCGCC GCAGTGACCT GTACGAGTCT 
GAGCTCAAAG AGTCCCGTCA GACCAGTGAG GAGCTCAAGA GGAAGGCAGT GGAGTATCAG 
CAGAAGATCC AGAAGGCTAA AGAACAAGGG AAAGTTGAGA TGGAGGAGCT TCTGAGCAAA 
CTGCAGAAGA CCAATGGAGA GCAACAAGTA AAGATTGAAG AGCTCCAGGA GAAGCTTGCT 
AAGGCAGTGA AGGCGAGTAC TGAAGCCACT GAACTCCTCC AGAATGTCCG TCAGGCTAAA 
GAGCGAATGG AGCGAGACCT GGAGCGTCTG CAGACCAAAG AGGACTCGTC CGACAGCCTG 
CGCAGACGCT TACGAGAGAC TGAGGATGGC AAAAAATCCC TGGAGAACCA GGTGAAGAGG 
CTGGAGATGG TGGAGCGGAG AGAATCCAAA CTGAAAGACG ACATTCAGAC CAAATCGCAG 
CAGATCCAGC AGATGGCCGA CAAAATCATG GAGCTTGAAG AAAATTTGCG GGACTCGCAG 
GCGATGGCGC AGCGCATGGA AACACAGCTG GAGCAGAAGG AAAAGCTTTT TGAAGACAAG 
ATCAAGGTTT TAGAAACTCA AATGAAGGCT GATTTGGCTG AGAAGGATTT GCTGGAGTCC 
AAACAGAGCA CTTTTGAAGA GGAGTCTCGC GAACGGGGAA AAGTGATCAG CGACCAGGCA 
GCGACGATCA ATGCCATGGA GTCAAAGATG ACCAGTCTGG AGCAGAGGAT CGCTGAGCTG 
TCCGAAGCCA ACAAACTGGC AGCAAACAGC AGCATTTACA CACAGAAGAA CATGAAAGCC 
CAGGAGGAGA TGATTTCTGA ACTCAGACAG CAGAAGTTTT ATTTGGAGTC TCAGGCTGGA 
AAACTGGAGG CGCAGAATGC AAAGCTGGAG GAGCATCTGG AGAAACTCAC CCAACAAGAG 
CAGACAAACA AGAGCCGAGT GCTGGAGCTG GAAGCCAGGC TGCGAGAGAT CGGCCTAGAG 
CATGAGGAAC AGAAGCTGGA GATCAAGCGT CAGGTGTCGG AGCTCACGTT GTCTCTGCAG 
GAGAGAGAAG CTCAGATCAG CAGCCTGCAA GCGGCACGCC ACGCTCTCGA CAGCCAACTA 
CAGCAGGCCA AAACTGAGCT GGAGGAGACC ACCGCCGAGG CCGAAGAGGA GATCACCGTC 
CTGAGGGCCC ATCGGGATGA AATCCAACGC AAGTTTGATG CCCTGCGAGA CAGCTGTGCG 
GTGATCACGG ATCTGGAGGA GCAGTTGACG CAGCTCACGC AAGAAAACGC GGAGCTGAAC 
CGGCAGAACT TCTACCTGTC CAAACAGCTG GACGAGGCCA CAGACGAGAA CGAGGAGCGC 
CTGCAGCTCA AGCAGGACGT GGAGCGCCTC AGGAGAGAGG TGGCAGACCG AGAGATGCAC 
CTCAACAACC AGAAACAGGT GACACGGCAA CACACACACA CACACACACA CACACACACA 
CACAGGACAG AGGTGGCAGA CCGAGAGATG CACCTCAACA ACCAGAAACA GAACATCGAG 
ACGCTGAAGA CGACATGTAC AATGCTGGAG GAGCAGGTTA TGGAGCTGGA GACTCTGAAT 
GATGAGCTGA TGGAGAAGGA GAGGCAGTGG GAGAACTGGA GATCTGCTCT GGACGAGGAG 
AAAAACCAGG CCGAGCGCAG AGCCAGAGAC CTGCAAAGAC AGCTGGACAG CGAGAAGCAG 
AACCGACTGC GTGCAGAACA GAGAAACTCT GAGTCTCGGC AGGCCGTGGA GCTGGCGGTG 
AAAGAGCACA AAGCTGAAAT CCTGGTTCTG CAGCAGGCGC TCAAAGACCA GAAACTCAAG 
GCTGAGGGCC TTTCTGACAC TCTGGGTGAT CTGGAGAAGA AGCACGGCAT GCTGGAGATG 
AACGCTCGCA GCCTGCAGCA GAAGCTGGAG ACGGAGAGAG AGATGAAGCA GAGACTCATG 
GACGAGCAAG CTAAACTGCA GCAGCAGCTG GACATGCAGA AGAGCCACAT CTTCAGACTG 
ACTCAAGGAC TGCAGGATGC GCTGGACCAG ACCGACCTGC TGAAGACCGA GCGCACAGAC 
CTGGAGTACC AGCTGGAAAA CATACAGGCG GCGTATTCTC ACGAGAAGGT GAAGATGGAG 
GGGACGATCA CCCAGCAGAC CAAGCTCATA GACTTCCTGC AGGCCAAGGT AGAGCACCCC 
TCTAAGAAGA AAAAGGGGCT GTTTGGTGGC CGGCGGCGGG AGGATCTGCT AGCGGCGCTG 
GCGGCTCAGG GACAGACACA GGTGCCTGTG GTGCCCGCCG CCACTCCTGT GCCCCTGCAG 
TACAGCGACC TGAAGGCGGC GCTGGATAAA GAGCGAGCCC GATGCTCCGA GCTGGAGGAC 
GCCCTGCAGA AAACACGCCT GGAGCTCAGA GAAGTTCAGT TTAATAAAGC TCTGGAGCAC 
AGCTCTTCTC CAGCCACTCC AGGTTTGGCC CGTCAGCAGC TCATGATGTC CACCAAGTCC 
AACAAGTCTC CAGAGCACCA GTCCAGCGGC GGCCTGATGT CCTCAAACTC CAGCCGCGGA 
CGCAAAGACA ACGCCAATCC TGAAGAAATA AGGAGAGTCA CTTCTGAAAA GTACACCAGA 
CGTGTGAAGG ACCGGATTCA TCACAACACT CCTCACCGCT TCACGCAGGG GCTCAATATG 
AGAGCGGCTC GCTGTACTGT GTGTCTGGAC ACAGTCCATT TTGGACGTCA GGCTGCAACA 
TGCATTGAAT GTCACGCGTT GTGTCACCCA AAATGCTCCC CGTACCTCCC TGCCACCTGC 
GGCGTTCCCA CTGAATACGC GCTGCACCTC TCAGAGGCTC TGTGTCGGGA GAAGGGAAGC 
ACTTCCGGCC TGAAAGATAT GGGTGGACAT ATGAGGCTTG AAGGATGGCT GAAGCAGCCC 
AGGAACGGGA AGCGCGGGCA GGGCTGGGAG AGGAAGTATG TGGTGCTGGA TGGATATAAA 
GTGATTACAT TCGAGACAGA ACCAAGAGAC GAGTCAGTGA AGCCGCTGGA TGAGTTTGAG 
CTGTGTCTCT CTGATGGAGA AGTGACCGTT CATTCTGCTG TGGGTTCCTC TGAGCTCCCC 
AACACCGCTA AAACAGATGT GCCGTACGTG CTGAAGCTGG AGTCGTCTCC TCAGTCCACA 
TGCTGGCCCG GTCAGACTCT GTACCTCATG ACCTCGGGAT ACTCAGAGAA GCAGCGCTGG 
GTGGCAGTGC TGGAAGCCAT CGTGGCCAGC AGTCGCGGAG CCAGAGAGAA AGCTGAGGCA 
GATGCTAAAT TATTGGGTAA CTCTTTACTT AAGCTAGAAG GTGACGACCG TCTGGATATC 
AACTGCACCC TGCCGCTGAC CGACCAGATT GTGTTGGTGG GCTCTGAGGA GGGTCTCTAC 
GCACTGAATG TGATTAAGAA CTCCCTCACT CATATCCCGG GTCTGGGATC CGTGTTTCAG 
ATCCACATTA TCAAAGATCA TGACAAGCTG CTGATGATCG TGGGAGACGA GCGAGCGCTG 
TGTCTGGTGG AGATTAAGAA GGTGAAGCAG TCTTTATCCC AGTCTCACCT CCCCACACTG 
CCCGAGATTG TGCCGTATAT CTTTGAGACC GTCAAGGGCT GTCATCTGTT TGCCTCGGGC 
AGGATAGAAA ATGGCCCATG CATTTGTGCT GCGATGCCCA ATAAAGTCAC GATTCTACGA 
TACAATGACA ATCTTAACAA GTTCTGCATA CGGAAGGAGA TTGAAACTCT GGAGCCGTGC 
AGCTGCATCC ACTTCACCAG CTACAGCATC ATCATTGGTA CCAATAAATT CTACGAGATC 
GAGATGAAGC AGTGCGTTCT GGACGAGTTC CTGGACAAGA ATGACGTGTC GCTGGCGTCG 
GCTGTCTTCA CCGGATCCTC CCACAGTTTC CCCATCTCTA TCGTCCAGGT GTCCAGCACT 
CCGCAGAAGG AGGAATATCT GCTGTGTTTC CACGAGATCG GAGTTTTTGT GGATGCTTAC 
GGTCGAAGAA GTCGCCCTGA TGATCTGAAG TGGAGTCGAC TTCCCCTGAC GTTCGCCTTC 
AGAGAGCCGT ATCTCTTTGT GACCTACTTC AACTCTCTGG ATGTTATTGA GATTCAGGGA 
CACGCAGCTC TTGGGCCTCC CAGTCTGGTT CACCTGGACA TTCCTAACCC ACGTTATCTG 
GGTCCAGCCA TATCGTCTGG TGCCATCTAT CTGGCCTCGT CCTACCAGAA CAAACTGCGG 
GTGATCTGCT GTAAGGGCAG TCTGGTGCGG GAGTCTGGAG AGCTGCAGAG GACCGGCTCC 
AGCAGGAGTC AAAGCATCAT GCATTTACCC GAATCTCCAC AGCAGCCCCA ACAAGCGCGG 
TCCACCAACA TACAACGAGC ACATCTCCAA ACGTCTGGCC TCGAGTCCTG GAGCCCAGGA 
GGGGCCCCTG CACCGCGAGC CCAGCACTCC TCACCGCTAC CGCGAGGGCC GCACCGAGTT 
CCGGAGAGAC AAATCTCCAG CCCGGCCCCT GGAGCGAGAG AAATCCCCGG GCCGTCCGGT 
GCTAGACAGC CGCAGAGAGA GGTCTCCCGG TCGCTTCGAG AGCAGCGGCA GCGAGAGAGA 
CCGCGAGCGG ACACGACTGC ACACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301995.2
CDS	433..5379
Translation	MEQEISKVQRKMSDLESVLQQKDVELKASETQRNILEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSNKLQEIKEQEYHAQLEEMRVSIRQLEENLSAARRRSDLYESELKESRQTSEELKRKAVEYQQKIQKAKEQGKVEMEELLSKLQKTNGEQQVKIEELQEKLAKAVKASTEATELLQNVRQAKERMERDLERLQTKEDSSDSLRRRLRETEDGKKSLENQVKRLEMVERRESKLKDDIQTKSQQIQQMADKIMELEENLRDSQAMAQRMETQLEQKEKLFEDKIKVLETQMKADLAEKDLLESKQSTFEEESRERGKVISDQAATINAMESKMTSLEQRIAELSEANKLAANSSIYTQKNMKAQEEMISELRQQKFYLESQAGKLEAQNAKLEEHLEKLTQQEQTNKSRVLELEARLREIGLEHEEQKLEIKRQVSELTLSLQEREAQISSLQAARHALDSQLQQAKTELEETTAEAEEEITVLRAHRDEIQRKFDALRDSCAVITDLEEQLTQLTQENAELNRQNFYLSKQLDEATDENEERLQLKQDVERLRREVADREMHLNNQKQVTRQHTHTHTHTHTHRTEVADREMHLNNQKQNIETLKTTCTMLEEQVMELETLNDELMEKERQWENWRSALDEEKNQAERRARDLQRQLDSEKQNRLRAEQRNSESRQAVELAVKEHKAEILVLQQALKDQKLKAEGLSDTLGDLEKKHGMLEMNARSLQQKLETEREMKQRLMDEQAKLQQQLDMQKSHIFRLTQGLQDALDQTDLLKTERTDLEYQLENIQAAYSHEKVKMEGTITQQTKLIDFLQAKVEHPSKKKKGLFGGRRREDLLAALAAQGQTQVPVVPAATPVPLQYSDLKAALDKERARCSELEDALQKTRLELREVQFNKALEHSSSPATPGLARQQLMMSTKSNKSPEHQSSGGLMSSNSSRGRKDNANPEEIRRVTSEKYTRRVKDRIHHNTPHRFTQGLNMRAARCTVCLDTVHFGRQAATCIECHALCHPKCSPYLPATCGVPTEYALHLSEALCREKGSTSGLKDMGGHMRLEGWLKQPRNGKRGQGWERKYVVLDGYKVITFETEPRDESVKPLDEFELCLSDGEVTVHSAVGSSELPNTAKTDVPYVLKLESSPQSTCWPGQTLYLMTSGYSEKQRWVAVLEAIVASSRGAREKAEADAKLLGNSLLKLEGDDRLDINCTLPLTDQIVLVGSEEGLYALNVIKNSLTHIPGLGSVFQIHIIKDHDKLLMIVGDERALCLVEIKKVKQSLSQSHLPTLPEIVPYIFETVKGCHLFASGRIENGPCICAAMPNKVTILRYNDNLNKFCIRKEIETLEPCSCIHFTSYSIIIGTNKFYEIEMKQCVLDEFLDKNDVSLASAVFTGSSHSFPISIVQVSSTPQKEEYLLCFHEIGVFVDAYGRRSRPDDLKWSRLPLTFAFREPYLFVTYFNSLDVIEIQGHAALGPPSLVHLDIPNPRYLGPAISSGAIYLASSYQNKLRVICCKGSLVRESGELQRTGSSRSQSIMHLPESPQQPQQARSTNIQRAHLQTSGLESWSPGGAPAPRAQHSSPLPRGPHRVPERQISSPAPGAREIPGPSGARQPQREVSRSLREQRQRERPRADTTAH

Target ORF information:

RefSeq Version	XM_009303720.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303720.3

ORF Insert Sequence:

ATGGAGCAGG AGATTTCCAA AGTGCAGCGT AAGATGTCAG ACTTGGAGTC AGTTTTGCAG 
CAGAAAGACG TGGAGCTCAA GGCTTCAGAA ACCCAGAGAA ACATCTTAGA GCAAGACCTG 
GCAACCTATA TCACCGAATG CAGCAGTTTG AAGCGCAGTT TGGAGCAGGC CCGCATGGAG 
GTTTCGCAGG AAGATGATAA AGCTCTTCAG CTGCTGCACG ACATTCGAGA GCAGAGCAAC 
AAACTGCAGG AAATCAAAGA ACAGGAGTAC CACGCTCAGC TGGAGGAGAT GCGAGTGTCT 
ATTCGTCAGC TGGAAGAGAA TCTGTCGGCC GCTCGTCGCC GCAGTGACCT GTACGAGTCT 
GAGCTCAAAG AGTCCCGTCA GACCAGTGAG GAGCTCAAGA GGAAGGCAGT GGAGTATCAG 
CAGAAGATCC AGAAGGCTAA AGAACAAGGG AAAGTTGAGA TGGAGGAGCT TCTGAGCAAA 
CTGCAGAAGA CCAATGGAGA GCAACAAGTA AAGATTGAAG AGCTCCAGGA GAAGCTTGCT 
AAGGCAGTGA AGGCGAGTAC TGAAGCCACT GAACTCCTCC AGAATGTCCG TCAGGCTAAA 
GAGCGAATGG AGCGAGACCT GGAGCGTCTG CAGACCAAAG AGGACTCGTC CGACAGCCTG 
CGCAGACGCT TACGAGAGAC TGAGGATGGC AAAAAATCCC TGGAGAACCA GGTGAAGAGG 
CTGGAGATGG TGGAGCGGAG AGAATCCAAA CTGAAAGACG ACATTCAGAC CAAATCGCAG 
CAGATCCAGC AGATGGCCGA CAAAATCATG GAGCTTGAAG AAAATTTGCG GGACTCGCAG 
GCGATGGCGC AGCGCATGGA AACACAGCTG GAGCAGAAGG AAAAGCTTTT TGAAGACAAG 
ATCAAGGTTT TAGAAACTCA AATGAAGGCT GATTTGGCTG AGAAGGATTT GCTGGAGTCC 
AAACAGAGCA CTTTTGAAGA GGAGTCTCGC GAACGGGGAA AAGTGATCAG CGACCAGGCA 
GCGACGATCA ATGCCATGGA GTCAAAGATG ACCAGTCTGG AGCAGAGGAT CGCTGAGCTG 
TCCGAAGCCA ACAAACTGGC AGCAAACAGC AGCATTTACA CACAGAAGAA CATGAAAGCC 
CAGGAGGAGA TGATTTCTGA ACTCAGACAG CAGAAGTTTT ATTTGGAGTC TCAGGCTGGA 
AAACTGGAGG CGCAGAATGC AAAGCTGGAG GAGCATCTGG AGAAACTCAC CCAACAAGAG 
CAGACAAACA AGAGCCGAGT GCTGGAGCTG GAAGCCAGGC TGCGAGAGAT CGGCCTAGAG 
CATGAGGAAC AGAAGCTGGA GATCAAGCGT CAGGTGTCGG AGCTCACGTT GTCTCTGCAG 
GAGAGAGAAG CTCAGATCAG CAGCCTGCAA GCGGCACGCC ACGCTCTCGA CAGCCAACTA 
CAGCAGGCCA AAACTGAGCT GGAGGAGACC ACCGCCGAGG CCGAAGAGGA GATCACCGTC 
CTGAGGGCCC ATCGGGATGA AATCCAACGC AAGTTTGATG CCCTGCGAGA CAGCTGTGCG 
GTGATCACGG ATCTGGAGGA GCAGTTGACG CAGCTCACGC AAGAAAACGC GGAGCTGAAC 
CGGCAGAACT TCTACCTGTC CAAACAGCTG GACGAGGCCA CAGACGAGAA CGAGGAGCGC 
CTGCAGCTCA AGCAGGACGT GGAGCGCCTC AGGAGAGAGG TGGCAGACCG AGAGATGCAC 
CTCAACAACC AGAAACAGGT GACACGGCAA CACACACACA CACACACACA CACACACACA 
CACAGGACAG AGGTGGCAGA CCGAGAGATG CACCTCAACA ACCAGAAACA GAACATCGAG 
ACGCTGAAGA CGACATGTAC AATGCTGGAG GAGCAGGTTA TGGAGCTGGA GACTCTGAAT 
GATGAGCTGA TGGAGAAGGA GAGGCAGTGG GAGAACTGGA GATCTGCTCT GGACGAGGAG 
AAAAACCAGG CCGAGCGCAG AGCCAGAGAC CTGCAAAGAC AGCTGGACAG CGAGAAGCAG 
AACCGACTGC GTGCAGAACA GAGAAACTCT GAGTCTCGGC AGGCCGTGGA GCTGGCGGTG 
AAAGAGCACA AAGCTGAAAT CCTGGTTCTG CAGCAGGCGC TCAAAGACCA GAAACTCAAG 
GCTGAGGGCC TTTCTGACAC TCTGGGTGAT CTGGAGAAGA AGCACGGCAT GCTGGAGATG 
AACGCTCGCA GCCTGCAGCA GAAGCTGGAG ACGGAGAGAG AGATGAAGCA GAGACTCATG 
GACGAGCAAG CTAAACTGCA GCAGCAGCTG GACATGCAGA AGAGCCACAT CTTCAGACTG 
ACTCAAGGAC TGCAGGATGC GCTGGACCAG ACCGACCTGC TGAAGACCGA GCGCACAGAC 
CTGGAGTACC AGCTGGAAAA CATACAGGCG GCGTATTCTC ACGAGAAGGT GAAGATGGAG 
GGGACGATCA CCCAGCAGAC CAAGCTCATA GACTTCCTGC AGGCCAAGGT AGAGCACCCC 
TCTAAGAAGA AAAAGGGGCT GTTTGGTGGC CGGCGGCGGG AGGATCTGCT AGCGGCGCTG 
GCGGCTCAGG GACAGACACA GGTGCCTGTG GTGCCCGCCG CCACTCCTGT GCCCCTGCAG 
TACAGCGACC TGAAGGCGGC GCTGGATAAA GAGCGAGCCC GATGCTCCGA GCTGGAGGAC 
GCCCTGCAGA AAACACGCCT GGAGCTCAGA GAAGTTCAGT TTAATAAAGC TCTGGAGCAC 
AGCTCTTCTC CAGCCACTCC AGGTTTGGCC CGTCAGCAGC TCATGATGTC CACCAAGTCC 
AACAAGTCTC CAGAGCACCA GTCCAGCGGC GGCCTGATGT CCTCAAACTC CAGCCGCGGA 
CGCAAAGACA ACGCCAATCC TGAAGAAATA AGGAGAGTCA CTTCTGAAAA GTACACCAGA 
CGTGTGAAGG ACCGGATTCA TCACAACACT CCTCACCGCT TCACGCAGGG GCTCAATATG 
AGAGCGGCTC GCTGTACTGT GTGTCTGGAC ACAGTCCATT TTGGACGTCA GGCTGCAACA 
TGCATTGAAT GTCACGCGTT GTGTCACCCA AAATGCTCCC CGTACCTCCC TGCCACCTGC 
GGCGTTCCCA CTGAATACGC GCTGCACCTC TCAGAGGCTC TGTGTCGGGA GAAGGGAAGC 
ACTTCCGGCC TGAAAGATAT GGGTGGACAT ATGAGGCTTG AAGGATGGCT GAAGCAGCCC 
AGGAACGGGA AGCGCGGGCA GGGCTGGGAG AGGAAGTATG TGGTGCTGGA TGGATATAAA 
GTGATTACAT TCGAGACAGA ACCAAGAGAC GAGTCAGTGA AGCCGCTGGA TGAGTTTGAG 
CTGTGTCTCT CTGATGGAGA AGTGACCGTT CATTCTGCTG TGGGTTCCTC TGAGCTCCCC 
AACACCGCTA AAACAGATGT GCCGTACGTG CTGAAGCTGG AGTCGTCTCC TCAGTCCACA 
TGCTGGCCCG GTCAGACTCT GTACCTCATG ACCTCGGGAT ACTCAGAGAA GCAGCGCTGG 
GTGGCAGTGC TGGAAGCCAT CGTGGCCAGC AGTCGCGGAG CCAGAGAGAA AGCTGAGGCA 
GATGCTAAAT TATTGGGTAA CTCTTTACTT AAGCTAGAAG GTGACGACCG TCTGGATATC 
AACTGCACCC TGCCGCTGAC CGACCAGATT GTGTTGGTGG GCTCTGAGGA GGGTCTCTAC 
GCACTGAATG TGATTAAGAA CTCCCTCACT CATATCCCGG GTCTGGGATC CGTGTTTCAG 
ATCCACATTA TCAAAGATCA TGACAAGCTG CTGATGATCG TGGGAGACGA GCGAGCGCTG 
TGTCTGGTGG AGATTAAGAA GGTGAAGCAG TCTTTATCCC AGTCTCACCT CCCCACACTG 
CCCGAGATTG TGCCGTATAT CTTTGAGACC GTCAAGGGCT GTCATCTGTT TGCCTCGGGC 
AGGATAGAAA ATGGCCCATG CATTTGTGCT GCGATGCCCA ATAAAGTCAC GATTCTACGA 
TACAATGACA ATCTTAACAA GTTCTGCATA CGGAAGGAGA TTGAAACTCT GGAGCCGTGC 
AGCTGCATCC ACTTCACCAG CTACAGCATC ATCATTGGTA CCAATAAATT CTACGAGATC 
GAGATGAAGC AGTGCGTTCT GGACGAGTTC CTGGACAAGA ATGACGTGTC GCTGGCGTCG 
GCTGTCTTCA CCGGATCCTC CCACAGTTTC CCCATCTCTA TCGTCCAGGT GTCCAGCACT 
CCGCAGAAGG AGGAATATCT GCTGTGTTTC CACGAGATCG GAGTTTTTGT GGATGCTTAC 
GGTCGAAGAA GTCGCCCTGA TGATCTGAAG TGGAGTCGAC TTCCCCTGAC GTTCGCCTTC 
AGAGAGCCGT ATCTCTTTGT GACCTACTTC AACTCTCTGG ATGTTATTGA GATTCAGGGA 
CACGCAGCTC TTGGGCCTCC CAGTCTGGTT CACCTGGACA TTCCTAACCC ACGTTATCTG 
GGTCCAGCCA TATCGTCTGG TGCCATCTAT CTGGCCTCGT CCTACCAGAA CAAACTGCGG 
GTGATCTGCT GTAAGGGCAG TCTGGTGCGG GAGTCTGGAG AGCTGCAGAG GACCGGCTCC 
AGCAGGAGTC AAAGCATCAT GCATTTACCC GAATCTCCAC AGCAGCCCCA ACAAGCGCGG 
TCCACCAACA TACAACGAGC ACATCTCCAA ACGTCTGGCC TCGAGTCCTG GAGCCCAGGA 
GGGGCCCCTG CACCGCGAGC CCAGCACTCC TCACCGCTAC CGCGAGGGCC GCACCGAGTT 
CCGGAGAGAC AAATCTCCAG CCCGGCCCCT GGAGCGAGAG AAATCCCCGG GCCGTCCGGT 
GCTAGACAGC CGCAGAGAGA GGTCTCCCGG TCGCTTCGAG AGCAGCGGCA GCGAGAGAGA 
CCGCGAGCGG ACACGACTGC ACACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100333880 ( XM_009303720.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301995.2 Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa60007
Clone ID Related Accession (Same CDS sequence)	XM_009303720.3 , XM_009303720.2
Accession Version	XM_009303720.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	citron Rho-interacting kinase-like isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303720.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG AGATTTCCAA AGTGCAGCGT AAGATGTCAG ACTTGGAGTC AGTTTTGCAG 
CAGAAAGACG TGGAGCTCAA GGCTTCAGAA ACCCAGAGAA ACATCTTAGA GCAAGACCTG 
GCAACCTATA TCACCGAATG CAGCAGTTTG AAGCGCAGTT TGGAGCAGGC CCGCATGGAG 
GTTTCGCAGG AAGATGATAA AGCTCTTCAG CTGCTGCACG ACATTCGAGA GCAGAGCAAC 
AAACTGCAGG AAATCAAAGA ACAGGAGTAC CACGCTCAGC TGGAGGAGAT GCGAGTGTCT 
ATTCGTCAGC TGGAAGAGAA TCTGTCGGCC GCTCGTCGCC GCAGTGACCT GTACGAGTCT 
GAGCTCAAAG AGTCCCGTCA GACCAGTGAG GAGCTCAAGA GGAAGGCAGT GGAGTATCAG 
CAGAAGATCC AGAAGGCTAA AGAACAAGGG AAAGTTGAGA TGGAGGAGCT TCTGAGCAAA 
CTGCAGAAGA CCAATGGAGA GCAACAAGTA AAGATTGAAG AGCTCCAGGA GAAGCTTGCT 
AAGGCAGTGA AGGCGAGTAC TGAAGCCACT GAACTCCTCC AGAATGTCCG TCAGGCTAAA 
GAGCGAATGG AGCGAGACCT GGAGCGTCTG CAGACCAAAG AGGACTCGTC CGACAGCCTG 
CGCAGACGCT TACGAGAGAC TGAGGATGGC AAAAAATCCC TGGAGAACCA GGTGAAGAGG 
CTGGAGATGG TGGAGCGGAG AGAATCCAAA CTGAAAGACG ACATTCAGAC CAAATCGCAG 
CAGATCCAGC AGATGGCCGA CAAAATCATG GAGCTTGAAG AAAATTTGCG GGACTCGCAG 
GCGATGGCGC AGCGCATGGA AACACAGCTG GAGCAGAAGG AAAAGCTTTT TGAAGACAAG 
ATCAAGGTTT TAGAAACTCA AATGAAGGCT GATTTGGCTG AGAAGGATTT GCTGGAGTCC 
AAACAGAGCA CTTTTGAAGA GGAGTCTCGC GAACGGGGAA AAGTGATCAG CGACCAGGCA 
GCGACGATCA ATGCCATGGA GTCAAAGATG ACCAGTCTGG AGCAGAGGAT CGCTGAGCTG 
TCCGAAGCCA ACAAACTGGC AGCAAACAGC AGCATTTACA CACAGAAGAA CATGAAAGCC 
CAGGAGGAGA TGATTTCTGA ACTCAGACAG CAGAAGTTTT ATTTGGAGTC TCAGGCTGGA 
AAACTGGAGG CGCAGAATGC AAAGCTGGAG GAGCATCTGG AGAAACTCAC CCAACAAGAG 
CAGACAAACA AGAGCCGAGT GCTGGAGCTG GAAGCCAGGC TGCGAGAGAT CGGCCTAGAG 
CATGAGGAAC AGAAGCTGGA GATCAAGCGT CAGGTGTCGG AGCTCACGTT GTCTCTGCAG 
GAGAGAGAAG CTCAGATCAG CAGCCTGCAA GCGGCACGCC ACGCTCTCGA CAGCCAACTA 
CAGCAGGCCA AAACTGAGCT GGAGGAGACC ACCGCCGAGG CCGAAGAGGA GATCACCGTC 
CTGAGGGCCC ATCGGGATGA AATCCAACGC AAGTTTGATG CCCTGCGAGA CAGCTGTGCG 
GTGATCACGG ATCTGGAGGA GCAGTTGACG CAGCTCACGC AAGAAAACGC GGAGCTGAAC 
CGGCAGAACT TCTACCTGTC CAAACAGCTG GACGAGGCCA CAGACGAGAA CGAGGAGCGC 
CTGCAGCTCA AGCAGGACGT GGAGCGCCTC AGGAGAGAGG TGGCAGACCG AGAGATGCAC 
CTCAACAACC AGAAACAGGT GACACGGCAA CACACACACA CACACACACA CACACACACA 
CACAGGACAG AGGTGGCAGA CCGAGAGATG CACCTCAACA ACCAGAAACA GAACATCGAG 
ACGCTGAAGA CGACATGTAC AATGCTGGAG GAGCAGGTTA TGGAGCTGGA GACTCTGAAT 
GATGAGCTGA TGGAGAAGGA GAGGCAGTGG GAGAACTGGA GATCTGCTCT GGACGAGGAG 
AAAAACCAGG CCGAGCGCAG AGCCAGAGAC CTGCAAAGAC AGCTGGACAG CGAGAAGCAG 
AACCGACTGC GTGCAGAACA GAGAAACTCT GAGTCTCGGC AGGCCGTGGA GCTGGCGGTG 
AAAGAGCACA AAGCTGAAAT CCTGGTTCTG CAGCAGGCGC TCAAAGACCA GAAACTCAAG 
GCTGAGGGCC TTTCTGACAC TCTGGGTGAT CTGGAGAAGA AGCACGGCAT GCTGGAGATG 
AACGCTCGCA GCCTGCAGCA GAAGCTGGAG ACGGAGAGAG AGATGAAGCA GAGACTCATG 
GACGAGCAAG CTAAACTGCA GCAGCAGCTG GACATGCAGA AGAGCCACAT CTTCAGACTG 
ACTCAAGGAC TGCAGGATGC GCTGGACCAG ACCGACCTGC TGAAGACCGA GCGCACAGAC 
CTGGAGTACC AGCTGGAAAA CATACAGGCG GCGTATTCTC ACGAGAAGGT GAAGATGGAG 
GGGACGATCA CCCAGCAGAC CAAGCTCATA GACTTCCTGC AGGCCAAGGT AGAGCACCCC 
TCTAAGAAGA AAAAGGGGCT GTTTGGTGGC CGGCGGCGGG AGGATCTGCT AGCGGCGCTG 
GCGGCTCAGG GACAGACACA GGTGCCTGTG GTGCCCGCCG CCACTCCTGT GCCCCTGCAG 
TACAGCGACC TGAAGGCGGC GCTGGATAAA GAGCGAGCCC GATGCTCCGA GCTGGAGGAC 
GCCCTGCAGA AAACACGCCT GGAGCTCAGA GAAGTTCAGT TTAATAAAGC TCTGGAGCAC 
AGCTCTTCTC CAGCCACTCC AGGTTTGGCC CGTCAGCAGC TCATGATGTC CACCAAGTCC 
AACAAGTCTC CAGAGCACCA GTCCAGCGGC GGCCTGATGT CCTCAAACTC CAGCCGCGGA 
CGCAAAGACA ACGCCAATCC TGAAGAAATA AGGAGAGTCA CTTCTGAAAA GTACACCAGA 
CGTGTGAAGG ACCGGATTCA TCACAACACT CCTCACCGCT TCACGCAGGG GCTCAATATG 
AGAGCGGCTC GCTGTACTGT GTGTCTGGAC ACAGTCCATT TTGGACGTCA GGCTGCAACA 
TGCATTGAAT GTCACGCGTT GTGTCACCCA AAATGCTCCC CGTACCTCCC TGCCACCTGC 
GGCGTTCCCA CTGAATACGC GCTGCACCTC TCAGAGGCTC TGTGTCGGGA GAAGGGAAGC 
ACTTCCGGCC TGAAAGATAT GGGTGGACAT ATGAGGCTTG AAGGATGGCT GAAGCAGCCC 
AGGAACGGGA AGCGCGGGCA GGGCTGGGAG AGGAAGTATG TGGTGCTGGA TGGATATAAA 
GTGATTACAT TCGAGACAGA ACCAAGAGAC GAGTCAGTGA AGCCGCTGGA TGAGTTTGAG 
CTGTGTCTCT CTGATGGAGA AGTGACCGTT CATTCTGCTG TGGGTTCCTC TGAGCTCCCC 
AACACCGCTA AAACAGATGT GCCGTACGTG CTGAAGCTGG AGTCGTCTCC TCAGTCCACA 
TGCTGGCCCG GTCAGACTCT GTACCTCATG ACCTCGGGAT ACTCAGAGAA GCAGCGCTGG 
GTGGCAGTGC TGGAAGCCAT CGTGGCCAGC AGTCGCGGAG CCAGAGAGAA AGCTGAGGCA 
GATGCTAAAT TATTGGGTAA CTCTTTACTT AAGCTAGAAG GTGACGACCG TCTGGATATC 
AACTGCACCC TGCCGCTGAC CGACCAGATT GTGTTGGTGG GCTCTGAGGA GGGTCTCTAC 
GCACTGAATG TGATTAAGAA CTCCCTCACT CATATCCCGG GTCTGGGATC CGTGTTTCAG 
ATCCACATTA TCAAAGATCA TGACAAGCTG CTGATGATCG TGGGAGACGA GCGAGCGCTG 
TGTCTGGTGG AGATTAAGAA GGTGAAGCAG TCTTTATCCC AGTCTCACCT CCCCACACTG 
CCCGAGATTG TGCCGTATAT CTTTGAGACC GTCAAGGGCT GTCATCTGTT TGCCTCGGGC 
AGGATAGAAA ATGGCCCATG CATTTGTGCT GCGATGCCCA ATAAAGTCAC GATTCTACGA 
TACAATGACA ATCTTAACAA GTTCTGCATA CGGAAGGAGA TTGAAACTCT GGAGCCGTGC 
AGCTGCATCC ACTTCACCAG CTACAGCATC ATCATTGGTA CCAATAAATT CTACGAGATC 
GAGATGAAGC AGTGCGTTCT GGACGAGTTC CTGGACAAGA ATGACGTGTC GCTGGCGTCG 
GCTGTCTTCA CCGGATCCTC CCACAGTTTC CCCATCTCTA TCGTCCAGGT GTCCAGCACT 
CCGCAGAAGG AGGAATATCT GCTGTGTTTC CACGAGATCG GAGTTTTTGT GGATGCTTAC 
GGTCGAAGAA GTCGCCCTGA TGATCTGAAG TGGAGTCGAC TTCCCCTGAC GTTCGCCTTC 
AGAGAGCCGT ATCTCTTTGT GACCTACTTC AACTCTCTGG ATGTTATTGA GATTCAGGGA 
CACGCAGCTC TTGGGCCTCC CAGTCTGGTT CACCTGGACA TTCCTAACCC ACGTTATCTG 
GGTCCAGCCA TATCGTCTGG TGCCATCTAT CTGGCCTCGT CCTACCAGAA CAAACTGCGG 
GTGATCTGCT GTAAGGGCAG TCTGGTGCGG GAGTCTGGAG AGCTGCAGAG GACCGGCTCC 
AGCAGGAGTC AAAGCATCAT GCATTTACCC GAATCTCCAC AGCAGCCCCA ACAAGCGCGG 
TCCACCAACA TACAACGAGC ACATCTCCAA ACGTCTGGCC TCGAGTCCTG GAGCCCAGGA 
GGGGCCCCTG CACCGCGAGC CCAGCACTCC TCACCGCTAC CGCGAGGGCC GCACCGAGTT 
CCGGAGAGAC AAATCTCCAG CCCGGCCCCT GGAGCGAGAG AAATCCCCGG GCCGTCCGGT 
GCTAGACAGC CGCAGAGAGA GGTCTCCCGG TCGCTTCGAG AGCAGCGGCA GCGAGAGAGA 
CCGCGAGCGG ACACGACTGC ACACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301995.2
CDS	434..5380
Translation	MEQEISKVQRKMSDLESVLQQKDVELKASETQRNILEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSNKLQEIKEQEYHAQLEEMRVSIRQLEENLSAARRRSDLYESELKESRQTSEELKRKAVEYQQKIQKAKEQGKVEMEELLSKLQKTNGEQQVKIEELQEKLAKAVKASTEATELLQNVRQAKERMERDLERLQTKEDSSDSLRRRLRETEDGKKSLENQVKRLEMVERRESKLKDDIQTKSQQIQQMADKIMELEENLRDSQAMAQRMETQLEQKEKLFEDKIKVLETQMKADLAEKDLLESKQSTFEEESRERGKVISDQAATINAMESKMTSLEQRIAELSEANKLAANSSIYTQKNMKAQEEMISELRQQKFYLESQAGKLEAQNAKLEEHLEKLTQQEQTNKSRVLELEARLREIGLEHEEQKLEIKRQVSELTLSLQEREAQISSLQAARHALDSQLQQAKTELEETTAEAEEEITVLRAHRDEIQRKFDALRDSCAVITDLEEQLTQLTQENAELNRQNFYLSKQLDEATDENEERLQLKQDVERLRREVADREMHLNNQKQVTRQHTHTHTHTHTHRTEVADREMHLNNQKQNIETLKTTCTMLEEQVMELETLNDELMEKERQWENWRSALDEEKNQAERRARDLQRQLDSEKQNRLRAEQRNSESRQAVELAVKEHKAEILVLQQALKDQKLKAEGLSDTLGDLEKKHGMLEMNARSLQQKLETEREMKQRLMDEQAKLQQQLDMQKSHIFRLTQGLQDALDQTDLLKTERTDLEYQLENIQAAYSHEKVKMEGTITQQTKLIDFLQAKVEHPSKKKKGLFGGRRREDLLAALAAQGQTQVPVVPAATPVPLQYSDLKAALDKERARCSELEDALQKTRLELREVQFNKALEHSSSPATPGLARQQLMMSTKSNKSPEHQSSGGLMSSNSSRGRKDNANPEEIRRVTSEKYTRRVKDRIHHNTPHRFTQGLNMRAARCTVCLDTVHFGRQAATCIECHALCHPKCSPYLPATCGVPTEYALHLSEALCREKGSTSGLKDMGGHMRLEGWLKQPRNGKRGQGWERKYVVLDGYKVITFETEPRDESVKPLDEFELCLSDGEVTVHSAVGSSELPNTAKTDVPYVLKLESSPQSTCWPGQTLYLMTSGYSEKQRWVAVLEAIVASSRGAREKAEADAKLLGNSLLKLEGDDRLDINCTLPLTDQIVLVGSEEGLYALNVIKNSLTHIPGLGSVFQIHIIKDHDKLLMIVGDERALCLVEIKKVKQSLSQSHLPTLPEIVPYIFETVKGCHLFASGRIENGPCICAAMPNKVTILRYNDNLNKFCIRKEIETLEPCSCIHFTSYSIIIGTNKFYEIEMKQCVLDEFLDKNDVSLASAVFTGSSHSFPISIVQVSSTPQKEEYLLCFHEIGVFVDAYGRRSRPDDLKWSRLPLTFAFREPYLFVTYFNSLDVIEIQGHAALGPPSLVHLDIPNPRYLGPAISSGAIYLASSYQNKLRVICCKGSLVRESGELQRTGSSRSQSIMHLPESPQQPQQARSTNIQRAHLQTSGLESWSPGGAPAPRAQHSSPLPRGPHRVPERQISSPAPGAREIPGPSGARQPQREVSRSLREQRQRERPRADTTAH

Target ORF information:

RefSeq Version	XM_009303720.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303720.2

ORF Insert Sequence:

ATGGAGCAGG AGATTTCCAA AGTGCAGCGT AAGATGTCAG ACTTGGAGTC AGTTTTGCAG 
CAGAAAGACG TGGAGCTCAA GGCTTCAGAA ACCCAGAGAA ACATCTTAGA GCAAGACCTG 
GCAACCTATA TCACCGAATG CAGCAGTTTG AAGCGCAGTT TGGAGCAGGC CCGCATGGAG 
GTTTCGCAGG AAGATGATAA AGCTCTTCAG CTGCTGCACG ACATTCGAGA GCAGAGCAAC 
AAACTGCAGG AAATCAAAGA ACAGGAGTAC CACGCTCAGC TGGAGGAGAT GCGAGTGTCT 
ATTCGTCAGC TGGAAGAGAA TCTGTCGGCC GCTCGTCGCC GCAGTGACCT GTACGAGTCT 
GAGCTCAAAG AGTCCCGTCA GACCAGTGAG GAGCTCAAGA GGAAGGCAGT GGAGTATCAG 
CAGAAGATCC AGAAGGCTAA AGAACAAGGG AAAGTTGAGA TGGAGGAGCT TCTGAGCAAA 
CTGCAGAAGA CCAATGGAGA GCAACAAGTA AAGATTGAAG AGCTCCAGGA GAAGCTTGCT 
AAGGCAGTGA AGGCGAGTAC TGAAGCCACT GAACTCCTCC AGAATGTCCG TCAGGCTAAA 
GAGCGAATGG AGCGAGACCT GGAGCGTCTG CAGACCAAAG AGGACTCGTC CGACAGCCTG 
CGCAGACGCT TACGAGAGAC TGAGGATGGC AAAAAATCCC TGGAGAACCA GGTGAAGAGG 
CTGGAGATGG TGGAGCGGAG AGAATCCAAA CTGAAAGACG ACATTCAGAC CAAATCGCAG 
CAGATCCAGC AGATGGCCGA CAAAATCATG GAGCTTGAAG AAAATTTGCG GGACTCGCAG 
GCGATGGCGC AGCGCATGGA AACACAGCTG GAGCAGAAGG AAAAGCTTTT TGAAGACAAG 
ATCAAGGTTT TAGAAACTCA AATGAAGGCT GATTTGGCTG AGAAGGATTT GCTGGAGTCC 
AAACAGAGCA CTTTTGAAGA GGAGTCTCGC GAACGGGGAA AAGTGATCAG CGACCAGGCA 
GCGACGATCA ATGCCATGGA GTCAAAGATG ACCAGTCTGG AGCAGAGGAT CGCTGAGCTG 
TCCGAAGCCA ACAAACTGGC AGCAAACAGC AGCATTTACA CACAGAAGAA CATGAAAGCC 
CAGGAGGAGA TGATTTCTGA ACTCAGACAG CAGAAGTTTT ATTTGGAGTC TCAGGCTGGA 
AAACTGGAGG CGCAGAATGC AAAGCTGGAG GAGCATCTGG AGAAACTCAC CCAACAAGAG 
CAGACAAACA AGAGCCGAGT GCTGGAGCTG GAAGCCAGGC TGCGAGAGAT CGGCCTAGAG 
CATGAGGAAC AGAAGCTGGA GATCAAGCGT CAGGTGTCGG AGCTCACGTT GTCTCTGCAG 
GAGAGAGAAG CTCAGATCAG CAGCCTGCAA GCGGCACGCC ACGCTCTCGA CAGCCAACTA 
CAGCAGGCCA AAACTGAGCT GGAGGAGACC ACCGCCGAGG CCGAAGAGGA GATCACCGTC 
CTGAGGGCCC ATCGGGATGA AATCCAACGC AAGTTTGATG CCCTGCGAGA CAGCTGTGCG 
GTGATCACGG ATCTGGAGGA GCAGTTGACG CAGCTCACGC AAGAAAACGC GGAGCTGAAC 
CGGCAGAACT TCTACCTGTC CAAACAGCTG GACGAGGCCA CAGACGAGAA CGAGGAGCGC 
CTGCAGCTCA AGCAGGACGT GGAGCGCCTC AGGAGAGAGG TGGCAGACCG AGAGATGCAC 
CTCAACAACC AGAAACAGGT GACACGGCAA CACACACACA CACACACACA CACACACACA 
CACAGGACAG AGGTGGCAGA CCGAGAGATG CACCTCAACA ACCAGAAACA GAACATCGAG 
ACGCTGAAGA CGACATGTAC AATGCTGGAG GAGCAGGTTA TGGAGCTGGA GACTCTGAAT 
GATGAGCTGA TGGAGAAGGA GAGGCAGTGG GAGAACTGGA GATCTGCTCT GGACGAGGAG 
AAAAACCAGG CCGAGCGCAG AGCCAGAGAC CTGCAAAGAC AGCTGGACAG CGAGAAGCAG 
AACCGACTGC GTGCAGAACA GAGAAACTCT GAGTCTCGGC AGGCCGTGGA GCTGGCGGTG 
AAAGAGCACA AAGCTGAAAT CCTGGTTCTG CAGCAGGCGC TCAAAGACCA GAAACTCAAG 
GCTGAGGGCC TTTCTGACAC TCTGGGTGAT CTGGAGAAGA AGCACGGCAT GCTGGAGATG 
AACGCTCGCA GCCTGCAGCA GAAGCTGGAG ACGGAGAGAG AGATGAAGCA GAGACTCATG 
GACGAGCAAG CTAAACTGCA GCAGCAGCTG GACATGCAGA AGAGCCACAT CTTCAGACTG 
ACTCAAGGAC TGCAGGATGC GCTGGACCAG ACCGACCTGC TGAAGACCGA GCGCACAGAC 
CTGGAGTACC AGCTGGAAAA CATACAGGCG GCGTATTCTC ACGAGAAGGT GAAGATGGAG 
GGGACGATCA CCCAGCAGAC CAAGCTCATA GACTTCCTGC AGGCCAAGGT AGAGCACCCC 
TCTAAGAAGA AAAAGGGGCT GTTTGGTGGC CGGCGGCGGG AGGATCTGCT AGCGGCGCTG 
GCGGCTCAGG GACAGACACA GGTGCCTGTG GTGCCCGCCG CCACTCCTGT GCCCCTGCAG 
TACAGCGACC TGAAGGCGGC GCTGGATAAA GAGCGAGCCC GATGCTCCGA GCTGGAGGAC 
GCCCTGCAGA AAACACGCCT GGAGCTCAGA GAAGTTCAGT TTAATAAAGC TCTGGAGCAC 
AGCTCTTCTC CAGCCACTCC AGGTTTGGCC CGTCAGCAGC TCATGATGTC CACCAAGTCC 
AACAAGTCTC CAGAGCACCA GTCCAGCGGC GGCCTGATGT CCTCAAACTC CAGCCGCGGA 
CGCAAAGACA ACGCCAATCC TGAAGAAATA AGGAGAGTCA CTTCTGAAAA GTACACCAGA 
CGTGTGAAGG ACCGGATTCA TCACAACACT CCTCACCGCT TCACGCAGGG GCTCAATATG 
AGAGCGGCTC GCTGTACTGT GTGTCTGGAC ACAGTCCATT TTGGACGTCA GGCTGCAACA 
TGCATTGAAT GTCACGCGTT GTGTCACCCA AAATGCTCCC CGTACCTCCC TGCCACCTGC 
GGCGTTCCCA CTGAATACGC GCTGCACCTC TCAGAGGCTC TGTGTCGGGA GAAGGGAAGC 
ACTTCCGGCC TGAAAGATAT GGGTGGACAT ATGAGGCTTG AAGGATGGCT GAAGCAGCCC 
AGGAACGGGA AGCGCGGGCA GGGCTGGGAG AGGAAGTATG TGGTGCTGGA TGGATATAAA 
GTGATTACAT TCGAGACAGA ACCAAGAGAC GAGTCAGTGA AGCCGCTGGA TGAGTTTGAG 
CTGTGTCTCT CTGATGGAGA AGTGACCGTT CATTCTGCTG TGGGTTCCTC TGAGCTCCCC 
AACACCGCTA AAACAGATGT GCCGTACGTG CTGAAGCTGG AGTCGTCTCC TCAGTCCACA 
TGCTGGCCCG GTCAGACTCT GTACCTCATG ACCTCGGGAT ACTCAGAGAA GCAGCGCTGG 
GTGGCAGTGC TGGAAGCCAT CGTGGCCAGC AGTCGCGGAG CCAGAGAGAA AGCTGAGGCA 
GATGCTAAAT TATTGGGTAA CTCTTTACTT AAGCTAGAAG GTGACGACCG TCTGGATATC 
AACTGCACCC TGCCGCTGAC CGACCAGATT GTGTTGGTGG GCTCTGAGGA GGGTCTCTAC 
GCACTGAATG TGATTAAGAA CTCCCTCACT CATATCCCGG GTCTGGGATC CGTGTTTCAG 
ATCCACATTA TCAAAGATCA TGACAAGCTG CTGATGATCG TGGGAGACGA GCGAGCGCTG 
TGTCTGGTGG AGATTAAGAA GGTGAAGCAG TCTTTATCCC AGTCTCACCT CCCCACACTG 
CCCGAGATTG TGCCGTATAT CTTTGAGACC GTCAAGGGCT GTCATCTGTT TGCCTCGGGC 
AGGATAGAAA ATGGCCCATG CATTTGTGCT GCGATGCCCA ATAAAGTCAC GATTCTACGA 
TACAATGACA ATCTTAACAA GTTCTGCATA CGGAAGGAGA TTGAAACTCT GGAGCCGTGC 
AGCTGCATCC ACTTCACCAG CTACAGCATC ATCATTGGTA CCAATAAATT CTACGAGATC 
GAGATGAAGC AGTGCGTTCT GGACGAGTTC CTGGACAAGA ATGACGTGTC GCTGGCGTCG 
GCTGTCTTCA CCGGATCCTC CCACAGTTTC CCCATCTCTA TCGTCCAGGT GTCCAGCACT 
CCGCAGAAGG AGGAATATCT GCTGTGTTTC CACGAGATCG GAGTTTTTGT GGATGCTTAC 
GGTCGAAGAA GTCGCCCTGA TGATCTGAAG TGGAGTCGAC TTCCCCTGAC GTTCGCCTTC 
AGAGAGCCGT ATCTCTTTGT GACCTACTTC AACTCTCTGG ATGTTATTGA GATTCAGGGA 
CACGCAGCTC TTGGGCCTCC CAGTCTGGTT CACCTGGACA TTCCTAACCC ACGTTATCTG 
GGTCCAGCCA TATCGTCTGG TGCCATCTAT CTGGCCTCGT CCTACCAGAA CAAACTGCGG 
GTGATCTGCT GTAAGGGCAG TCTGGTGCGG GAGTCTGGAG AGCTGCAGAG GACCGGCTCC 
AGCAGGAGTC AAAGCATCAT GCATTTACCC GAATCTCCAC AGCAGCCCCA ACAAGCGCGG 
TCCACCAACA TACAACGAGC ACATCTCCAA ACGTCTGGCC TCGAGTCCTG GAGCCCAGGA 
GGGGCCCCTG CACCGCGAGC CCAGCACTCC TCACCGCTAC CGCGAGGGCC GCACCGAGTT 
CCGGAGAGAC AAATCTCCAG CCCGGCCCCT GGAGCGAGAG AAATCCCCGG GCCGTCCGGT 
GCTAGACAGC CGCAGAGAGA GGTCTCCCGG TCGCTTCGAG AGCAGCGGCA GCGAGAGAGA 
CCGCGAGCGG ACACGACTGC ACACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100333880 ( XM_009303721.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301996.2 Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X7, mRNA.

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CloneID	ODa60008
Clone ID Related Accession (Same CDS sequence)	XM_009303721.3 , XM_009303721.2
Accession Version	XM_009303721.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	citron Rho-interacting kinase-like isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303721.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG AGATTTCCAA AGTGCAGCGT AAGATGTCAG ACTTGGAGTC AGTTTTGCAG 
CAGAAAGACG TGGAGCTCAA GGCTTCAGAA ACCCAGAGAA ACATCTTAGA GCAAGACCTG 
GCAACCTATA TCACCGAATG CAGCAGTTTG AAGCGCAGTT TGGAGCAGGC CCGCATGGAG 
GTTTCGCAGG AAGATGATAA AGCTCTTCAG CTGCTGCACG ACATTCGAGA GCAGAGCAAC 
AAACTGCAGG AAATCAAAGA ACAGGAGTAC CACGCTCAGC TGGAGGAGAT GCGAGTGTCT 
ATTCGTCAGC TGGAAGAGAA TCTGTCGGCC GCTCGTCGCC GCAGTGACCT GTACGAGTCT 
GAGCTCAAAG AGTCCCGTCA GACCAGTGAG GAGCTCAAGA GGAAGGCAGT GGAGTATCAG 
CAGAAGATCC AGAAGGCTAA AGAACAAGGG AAAGTTGAGA TGGAGGAGCT TCTGAGCAAA 
CTGCAGAAGA CCAATGGAGA GCAACAAGTA AAGATTGAAG AGCTCCAGGA GAAGCTTGCT 
AAGGCAGTGA AGGCGAGTAC TGAAGCCACT GAACTCCTCC AGAATGTCCG TCAGGCTAAA 
GAGCGAATGG AGCGAGACCT GGAGCGTCTG CAGACCAAAG AGGACTCGTC CGACAGCCTG 
CGCAGACGCT TACGAGAGAC TGAGGATGGC AAAAAATCCC TGGAGAACCA GGTGAAGAGG 
CTGGAGATGG TGGAGCGGAG AGAATCCAAA CTGAAAGACG ACATTCAGAC CAAATCGCAG 
CAGATCCAGC AGATGGCCGA CAAAATCATG GAGCTTGAAG AAAATTTGCG GGACTCGCAG 
GCGATGGCGC AGCGCATGGA AACACAGCTG GAGCAGAAGG AAAAGCTTTT TGAAGACAAG 
ATCAAGGTTT TAGAAACTCA AATGAAGGCT GATTTGGCTG AGAAGGATTT GCTGGAGTCC 
AAACAGAGCA CTTTTGAAGA GGAGTCTCGC GAACGGGGAA AAGTGATCAG CGACCAGGCA 
GCGACGATCA ATGCCATGGA GTCAAAGATG ACCAGTCTGG AGCAGAGGAT CGCTGAGCTG 
TCCGAAGCCA ACAAACTGGC AGCAAACAGC AGCATTTACA CACAGAAGAA CATGAAAGCC 
CAGGAGGAGA TGATTTCTGA ACTCAGACAG CAGAAGTTTT ATTTGGAGTC TCAGGCTGGA 
AAACTGGAGG CGCAGAATGC AAAGCTGGAG GAGCATCTGG AGAAACTCAC CCAACAAGAG 
CAGACAAACA AGAGCCGAGT GCTGGAGCTG GAAGCCAGGC TGCGAGAGAT CGGCCTAGAG 
CATGAGGAAC AGAAGCTGGA GATCAAGCGT CAGGTGTCGG AGCTCACGTT GTCTCTGCAG 
GAGAGAGAAG CTCAGATCAG CAGCCTGCAA GCGGCACGCC ACGCTCTCGA CAGCCAACTA 
CAGCAGGCCA AAACTGAGCT GGAGGAGACC ACCGCCGAGG CCGAAGAGGA GATCACCGTC 
CTGAGGGCCC ATCGGGATGA AATCCAACGC AAGTTTGATG CCCTGCGAGA CAGCTGTGCG 
GTGATCACGG ATCTGGAGGA GCAGTTGACG CAGCTCACGC AAGAAAACGC GGAGCTGAAC 
CGGCAGAACT TCTACCTGTC CAAACAGCTG GACGAGGCCA CAGACGAGAA CGAGGAGCGC 
CTGCAGCTCA AGCAGGACGT GGAGCGCCTC AGGAGAGAGG TGGCAGACCG AGAGATGCAC 
CTCAACAACC AGAAACAGGT GACACGGCAA CACACACACA CACACACACA CACACACACA 
CACAGGACAG AGGTGGCAGA CCGAGAGATG CACCTCAACA ACCAGAAACA GAACATCGAG 
ACGCTGAAGA CGACATGTAC AATGCTGGAG GAGCAGGTTA TGGAGCTGGA GACTCTGAAT 
GATGAGCTGA TGGAGAAGGA GAGGCAGTGG GAGAACTGGA GATCTGCTCT GGACGAGGAG 
AAAAACCAGG CCGAGCGCAG AGCCAGAGAC CTGCAAAGAC AGCTGGACAG CGAGAAGCAG 
AACCGACTGC GTGCAGAACA GAGAAACTCT GAGTCTCGGC AGGCCGTGGA GCTGGCGGTG 
AAAGAGCACA AAGCTGAAAT CCTGGTTCTG CAGCAGGCGC TCAAAGACCA GAAACTCAAG 
GCTGAGGGCC TTTCTGACAC TCTGGGTGAT CTGGAGAAGA AGCACGGCAT GCTGGAGATG 
AACGCTCGCA GCCTGCAGCA GAAGCTGGAG ACGGAGAGAG AGATGAAGCA GAGACTCATG 
GACGAGCAAG CTAAACTGCA GCAGCAGCTG GACATGCAGA AGAGCCACAT CTTCAGACTG 
ACTCAAGGAC TGCAGGATGC GCTGGACCAG ACCGACCTGC TGAAGACCGA GCGCACAGAC 
CTGGAGTACC AGCTGGAAAA CATACAGGCG GCGTATTCTC ACGAGAAGGT GAAGATGGAG 
GGGACGATCA CCCAGCAGAC CAAGCTCATA GACTTCCTGC AGGCCAAGGT AGAGCACCCC 
TCTAAGAAGA AAAAGGGGCT GTTTGGTGGC CGGCGGCGGG AGGATCTGCT AGCGGCGCTG 
GCGGCTCAGG GACAGACACA GGTGCCTGTG GTGCCCGCCG CCACTCCTGT GCCCCTGCAG 
TACAGCGACC TGAAGGCGGC GCTGGATAAA GAGCGAGCCC GATGCTCCGA GCTGGAGGAC 
GCCCTGCAGA AAACACGCCT GGAGCTCAGA GAAGTTCAGT TTAATAAAGC TCTGGAGCAC 
AGCTCTTCTC CAGCCACTCC AGGTTTGGCC CGTCAGCAGC TCATGATGTC CACCAAGTCC 
AACAAGTCTC CAGAGCACCA GTCCAGCGGC GGCCTGATGT CCTCAAACTC CAGCCGCGGA 
CGCAAAGACA ACGCCAATCC TGAAGAGTAC ACCAGACGTG TGAAGGACCG GATTCATCAC 
AACACTCCTC ACCGCTTCAC GCAGGGGCTC AATATGAGAG CGGCTCGCTG TACTGTGTGT 
CTGGACACAG TCCATTTTGG ACGTCAGGCT GCAACATGCA TTGAATGTCA CGCGTTGTGT 
CACCCAAAAT GCTCCCCGTA CCTCCCTGCC ACCTGCGGCG TTCCCACTGA ATACGCGCTG 
CACCTCTCAG AGGCTCTGTG TCGGGAGAAG GGAAGCACTT CCGGCCTGAA AGATATGGGT 
GGACATATGA GGCTTGAAGG ATGGCTGAAG CAGCCCAGGA ACGGGAAGCG CGGGCAGGGC 
TGGGAGAGGA AGTATGTGGT GCTGGATGGA TATAAAGTGA TTACATTCGA GACAGAACCA 
AGAGACGAGT CAGTGAAGCC GCTGGATGAG TTTGAGCTGT GTCTCTCTGA TGGAGAAGTG 
ACCGTTCATT CTGCTGTGGG TTCCTCTGAG CTCCCCAACA CCGCTAAAAC AGATGTGCCG 
TACGTGCTGA AGCTGGAGTC GTCTCCTCAG TCCACATGCT GGCCCGGTCA GACTCTGTAC 
CTCATGACCT CGGGATACTC AGAGAAGCAG CGCTGGGTGG CAGTGCTGGA AGCCATCGTG 
GCCAGCAGTC GCGGAGCCAG AGAGAAAGCT GAGGCAGATG CTAAATTATT GGGTAACTCT 
TTACTTAAGC TAGAAGGTGA CGACCGTCTG GATATCAACT GCACCCTGCC GCTGACCGAC 
CAGATTGTGT TGGTGGGCTC TGAGGAGGGT CTCTACGCAC TGAATGTGAT TAAGAACTCC 
CTCACTCATA TCCCGGGTCT GGGATCCGTG TTTCAGATCC ACATTATCAA AGATCATGAC 
AAGCTGCTGA TGATCGTGGG AGACGAGCGA GCGCTGTGTC TGGTGGAGAT TAAGAAGGTG 
AAGCAGTCTT TATCCCAGTC TCACCTCCCC ACACTGCCCG AGATTGTGCC GTATATCTTT 
GAGACCGTCA AGGGCTGTCA TCTGTTTGCC TCGGGCAGGA TAGAAAATGG CCCATGCATT 
TGTGCTGCGA TGCCCAATAA AGTCACGATT CTACGATACA ATGACAATCT TAACAAGTTC 
TGCATACGGA AGGAGATTGA AACTCTGGAG CCGTGCAGCT GCATCCACTT CACCAGCTAC 
AGCATCATCA TTGGTACCAA TAAATTCTAC GAGATCGAGA TGAAGCAGTG CGTTCTGGAC 
GAGTTCCTGG ACAAGAATGA CGTGTCGCTG GCGTCGGCTG TCTTCACCGG ATCCTCCCAC 
AGTTTCCCCA TCTCTATCGT CCAGGTGTCC AGCACTCCGC AGAAGGAGGA ATATCTGCTG 
TGTTTCCACG AGATCGGAGT TTTTGTGGAT GCTTACGGTC GAAGAAGTCG CCCTGATGAT 
CTGAAGTGGA GTCGACTTCC CCTGACGTTC GCCTTCAGAG AGCCGTATCT CTTTGTGACC 
TACTTCAACT CTCTGGATGT TATTGAGATT CAGGGACACG CAGCTCTTGG GCCTCCCAGT 
CTGGTTCACC TGGACATTCC TAACCCACGT TATCTGGGTC CAGCCATATC GTCTGGTGCC 
ATCTATCTGG CCTCGTCCTA CCAGAACAAA CTGCGGGTGA TCTGCTGTAA GGGCAGTCTG 
GTGCGGGAGT CTGGAGAGCT GCAGAGGACC GGCTCCAGCA GGAGCAGCCC CAACAAGCGC 
GGTCCACCAA CATACAACGA GCACATCTCC AAACGTCTGG CCTCGAGTCC TGGAGCCCAG 
GAGGGGCCCC TGCACCGCGA GCCCAGCACT CCTCACCGCT ACCGCGAGGG CCGCACCGAG 
TTCCGGAGAG ACAAATCTCC AGCCCGGCCC CTGGAGCGAG AGAAATCCCC GGGCCGTCCG 
GTGCTAGACA GCCGCAGAGA GAGGTCTCCC GGTCGCTTCG AGAGCAGCGG CAGCGAGAGA 
GACCGCGAGC GGACACGACT GCACACTAGC TCTGTACGCA CCCAGCTGAC CCCCGTCAAC 
AAGGTTTGGG ACCAGTCTTC GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301996.2
CDS	433..5379
Translation	MEQEISKVQRKMSDLESVLQQKDVELKASETQRNILEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSNKLQEIKEQEYHAQLEEMRVSIRQLEENLSAARRRSDLYESELKESRQTSEELKRKAVEYQQKIQKAKEQGKVEMEELLSKLQKTNGEQQVKIEELQEKLAKAVKASTEATELLQNVRQAKERMERDLERLQTKEDSSDSLRRRLRETEDGKKSLENQVKRLEMVERRESKLKDDIQTKSQQIQQMADKIMELEENLRDSQAMAQRMETQLEQKEKLFEDKIKVLETQMKADLAEKDLLESKQSTFEEESRERGKVISDQAATINAMESKMTSLEQRIAELSEANKLAANSSIYTQKNMKAQEEMISELRQQKFYLESQAGKLEAQNAKLEEHLEKLTQQEQTNKSRVLELEARLREIGLEHEEQKLEIKRQVSELTLSLQEREAQISSLQAARHALDSQLQQAKTELEETTAEAEEEITVLRAHRDEIQRKFDALRDSCAVITDLEEQLTQLTQENAELNRQNFYLSKQLDEATDENEERLQLKQDVERLRREVADREMHLNNQKQVTRQHTHTHTHTHTHRTEVADREMHLNNQKQNIETLKTTCTMLEEQVMELETLNDELMEKERQWENWRSALDEEKNQAERRARDLQRQLDSEKQNRLRAEQRNSESRQAVELAVKEHKAEILVLQQALKDQKLKAEGLSDTLGDLEKKHGMLEMNARSLQQKLETEREMKQRLMDEQAKLQQQLDMQKSHIFRLTQGLQDALDQTDLLKTERTDLEYQLENIQAAYSHEKVKMEGTITQQTKLIDFLQAKVEHPSKKKKGLFGGRRREDLLAALAAQGQTQVPVVPAATPVPLQYSDLKAALDKERARCSELEDALQKTRLELREVQFNKALEHSSSPATPGLARQQLMMSTKSNKSPEHQSSGGLMSSNSSRGRKDNANPEEYTRRVKDRIHHNTPHRFTQGLNMRAARCTVCLDTVHFGRQAATCIECHALCHPKCSPYLPATCGVPTEYALHLSEALCREKGSTSGLKDMGGHMRLEGWLKQPRNGKRGQGWERKYVVLDGYKVITFETEPRDESVKPLDEFELCLSDGEVTVHSAVGSSELPNTAKTDVPYVLKLESSPQSTCWPGQTLYLMTSGYSEKQRWVAVLEAIVASSRGAREKAEADAKLLGNSLLKLEGDDRLDINCTLPLTDQIVLVGSEEGLYALNVIKNSLTHIPGLGSVFQIHIIKDHDKLLMIVGDERALCLVEIKKVKQSLSQSHLPTLPEIVPYIFETVKGCHLFASGRIENGPCICAAMPNKVTILRYNDNLNKFCIRKEIETLEPCSCIHFTSYSIIIGTNKFYEIEMKQCVLDEFLDKNDVSLASAVFTGSSHSFPISIVQVSSTPQKEEYLLCFHEIGVFVDAYGRRSRPDDLKWSRLPLTFAFREPYLFVTYFNSLDVIEIQGHAALGPPSLVHLDIPNPRYLGPAISSGAIYLASSYQNKLRVICCKGSLVRESGELQRTGSSRSSPNKRGPPTYNEHISKRLASSPGAQEGPLHREPSTPHRYREGRTEFRRDKSPARPLEREKSPGRPVLDSRRERSPGRFESSGSERDRERTRLHTSSVRTQLTPVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_009303721.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303721.3

ORF Insert Sequence:

ATGGAGCAGG AGATTTCCAA AGTGCAGCGT AAGATGTCAG ACTTGGAGTC AGTTTTGCAG 
CAGAAAGACG TGGAGCTCAA GGCTTCAGAA ACCCAGAGAA ACATCTTAGA GCAAGACCTG 
GCAACCTATA TCACCGAATG CAGCAGTTTG AAGCGCAGTT TGGAGCAGGC CCGCATGGAG 
GTTTCGCAGG AAGATGATAA AGCTCTTCAG CTGCTGCACG ACATTCGAGA GCAGAGCAAC 
AAACTGCAGG AAATCAAAGA ACAGGAGTAC CACGCTCAGC TGGAGGAGAT GCGAGTGTCT 
ATTCGTCAGC TGGAAGAGAA TCTGTCGGCC GCTCGTCGCC GCAGTGACCT GTACGAGTCT 
GAGCTCAAAG AGTCCCGTCA GACCAGTGAG GAGCTCAAGA GGAAGGCAGT GGAGTATCAG 
CAGAAGATCC AGAAGGCTAA AGAACAAGGG AAAGTTGAGA TGGAGGAGCT TCTGAGCAAA 
CTGCAGAAGA CCAATGGAGA GCAACAAGTA AAGATTGAAG AGCTCCAGGA GAAGCTTGCT 
AAGGCAGTGA AGGCGAGTAC TGAAGCCACT GAACTCCTCC AGAATGTCCG TCAGGCTAAA 
GAGCGAATGG AGCGAGACCT GGAGCGTCTG CAGACCAAAG AGGACTCGTC CGACAGCCTG 
CGCAGACGCT TACGAGAGAC TGAGGATGGC AAAAAATCCC TGGAGAACCA GGTGAAGAGG 
CTGGAGATGG TGGAGCGGAG AGAATCCAAA CTGAAAGACG ACATTCAGAC CAAATCGCAG 
CAGATCCAGC AGATGGCCGA CAAAATCATG GAGCTTGAAG AAAATTTGCG GGACTCGCAG 
GCGATGGCGC AGCGCATGGA AACACAGCTG GAGCAGAAGG AAAAGCTTTT TGAAGACAAG 
ATCAAGGTTT TAGAAACTCA AATGAAGGCT GATTTGGCTG AGAAGGATTT GCTGGAGTCC 
AAACAGAGCA CTTTTGAAGA GGAGTCTCGC GAACGGGGAA AAGTGATCAG CGACCAGGCA 
GCGACGATCA ATGCCATGGA GTCAAAGATG ACCAGTCTGG AGCAGAGGAT CGCTGAGCTG 
TCCGAAGCCA ACAAACTGGC AGCAAACAGC AGCATTTACA CACAGAAGAA CATGAAAGCC 
CAGGAGGAGA TGATTTCTGA ACTCAGACAG CAGAAGTTTT ATTTGGAGTC TCAGGCTGGA 
AAACTGGAGG CGCAGAATGC AAAGCTGGAG GAGCATCTGG AGAAACTCAC CCAACAAGAG 
CAGACAAACA AGAGCCGAGT GCTGGAGCTG GAAGCCAGGC TGCGAGAGAT CGGCCTAGAG 
CATGAGGAAC AGAAGCTGGA GATCAAGCGT CAGGTGTCGG AGCTCACGTT GTCTCTGCAG 
GAGAGAGAAG CTCAGATCAG CAGCCTGCAA GCGGCACGCC ACGCTCTCGA CAGCCAACTA 
CAGCAGGCCA AAACTGAGCT GGAGGAGACC ACCGCCGAGG CCGAAGAGGA GATCACCGTC 
CTGAGGGCCC ATCGGGATGA AATCCAACGC AAGTTTGATG CCCTGCGAGA CAGCTGTGCG 
GTGATCACGG ATCTGGAGGA GCAGTTGACG CAGCTCACGC AAGAAAACGC GGAGCTGAAC 
CGGCAGAACT TCTACCTGTC CAAACAGCTG GACGAGGCCA CAGACGAGAA CGAGGAGCGC 
CTGCAGCTCA AGCAGGACGT GGAGCGCCTC AGGAGAGAGG TGGCAGACCG AGAGATGCAC 
CTCAACAACC AGAAACAGGT GACACGGCAA CACACACACA CACACACACA CACACACACA 
CACAGGACAG AGGTGGCAGA CCGAGAGATG CACCTCAACA ACCAGAAACA GAACATCGAG 
ACGCTGAAGA CGACATGTAC AATGCTGGAG GAGCAGGTTA TGGAGCTGGA GACTCTGAAT 
GATGAGCTGA TGGAGAAGGA GAGGCAGTGG GAGAACTGGA GATCTGCTCT GGACGAGGAG 
AAAAACCAGG CCGAGCGCAG AGCCAGAGAC CTGCAAAGAC AGCTGGACAG CGAGAAGCAG 
AACCGACTGC GTGCAGAACA GAGAAACTCT GAGTCTCGGC AGGCCGTGGA GCTGGCGGTG 
AAAGAGCACA AAGCTGAAAT CCTGGTTCTG CAGCAGGCGC TCAAAGACCA GAAACTCAAG 
GCTGAGGGCC TTTCTGACAC TCTGGGTGAT CTGGAGAAGA AGCACGGCAT GCTGGAGATG 
AACGCTCGCA GCCTGCAGCA GAAGCTGGAG ACGGAGAGAG AGATGAAGCA GAGACTCATG 
GACGAGCAAG CTAAACTGCA GCAGCAGCTG GACATGCAGA AGAGCCACAT CTTCAGACTG 
ACTCAAGGAC TGCAGGATGC GCTGGACCAG ACCGACCTGC TGAAGACCGA GCGCACAGAC 
CTGGAGTACC AGCTGGAAAA CATACAGGCG GCGTATTCTC ACGAGAAGGT GAAGATGGAG 
GGGACGATCA CCCAGCAGAC CAAGCTCATA GACTTCCTGC AGGCCAAGGT AGAGCACCCC 
TCTAAGAAGA AAAAGGGGCT GTTTGGTGGC CGGCGGCGGG AGGATCTGCT AGCGGCGCTG 
GCGGCTCAGG GACAGACACA GGTGCCTGTG GTGCCCGCCG CCACTCCTGT GCCCCTGCAG 
TACAGCGACC TGAAGGCGGC GCTGGATAAA GAGCGAGCCC GATGCTCCGA GCTGGAGGAC 
GCCCTGCAGA AAACACGCCT GGAGCTCAGA GAAGTTCAGT TTAATAAAGC TCTGGAGCAC 
AGCTCTTCTC CAGCCACTCC AGGTTTGGCC CGTCAGCAGC TCATGATGTC CACCAAGTCC 
AACAAGTCTC CAGAGCACCA GTCCAGCGGC GGCCTGATGT CCTCAAACTC CAGCCGCGGA 
CGCAAAGACA ACGCCAATCC TGAAGAGTAC ACCAGACGTG TGAAGGACCG GATTCATCAC 
AACACTCCTC ACCGCTTCAC GCAGGGGCTC AATATGAGAG CGGCTCGCTG TACTGTGTGT 
CTGGACACAG TCCATTTTGG ACGTCAGGCT GCAACATGCA TTGAATGTCA CGCGTTGTGT 
CACCCAAAAT GCTCCCCGTA CCTCCCTGCC ACCTGCGGCG TTCCCACTGA ATACGCGCTG 
CACCTCTCAG AGGCTCTGTG TCGGGAGAAG GGAAGCACTT CCGGCCTGAA AGATATGGGT 
GGACATATGA GGCTTGAAGG ATGGCTGAAG CAGCCCAGGA ACGGGAAGCG CGGGCAGGGC 
TGGGAGAGGA AGTATGTGGT GCTGGATGGA TATAAAGTGA TTACATTCGA GACAGAACCA 
AGAGACGAGT CAGTGAAGCC GCTGGATGAG TTTGAGCTGT GTCTCTCTGA TGGAGAAGTG 
ACCGTTCATT CTGCTGTGGG TTCCTCTGAG CTCCCCAACA CCGCTAAAAC AGATGTGCCG 
TACGTGCTGA AGCTGGAGTC GTCTCCTCAG TCCACATGCT GGCCCGGTCA GACTCTGTAC 
CTCATGACCT CGGGATACTC AGAGAAGCAG CGCTGGGTGG CAGTGCTGGA AGCCATCGTG 
GCCAGCAGTC GCGGAGCCAG AGAGAAAGCT GAGGCAGATG CTAAATTATT GGGTAACTCT 
TTACTTAAGC TAGAAGGTGA CGACCGTCTG GATATCAACT GCACCCTGCC GCTGACCGAC 
CAGATTGTGT TGGTGGGCTC TGAGGAGGGT CTCTACGCAC TGAATGTGAT TAAGAACTCC 
CTCACTCATA TCCCGGGTCT GGGATCCGTG TTTCAGATCC ACATTATCAA AGATCATGAC 
AAGCTGCTGA TGATCGTGGG AGACGAGCGA GCGCTGTGTC TGGTGGAGAT TAAGAAGGTG 
AAGCAGTCTT TATCCCAGTC TCACCTCCCC ACACTGCCCG AGATTGTGCC GTATATCTTT 
GAGACCGTCA AGGGCTGTCA TCTGTTTGCC TCGGGCAGGA TAGAAAATGG CCCATGCATT 
TGTGCTGCGA TGCCCAATAA AGTCACGATT CTACGATACA ATGACAATCT TAACAAGTTC 
TGCATACGGA AGGAGATTGA AACTCTGGAG CCGTGCAGCT GCATCCACTT CACCAGCTAC 
AGCATCATCA TTGGTACCAA TAAATTCTAC GAGATCGAGA TGAAGCAGTG CGTTCTGGAC 
GAGTTCCTGG ACAAGAATGA CGTGTCGCTG GCGTCGGCTG TCTTCACCGG ATCCTCCCAC 
AGTTTCCCCA TCTCTATCGT CCAGGTGTCC AGCACTCCGC AGAAGGAGGA ATATCTGCTG 
TGTTTCCACG AGATCGGAGT TTTTGTGGAT GCTTACGGTC GAAGAAGTCG CCCTGATGAT 
CTGAAGTGGA GTCGACTTCC CCTGACGTTC GCCTTCAGAG AGCCGTATCT CTTTGTGACC 
TACTTCAACT CTCTGGATGT TATTGAGATT CAGGGACACG CAGCTCTTGG GCCTCCCAGT 
CTGGTTCACC TGGACATTCC TAACCCACGT TATCTGGGTC CAGCCATATC GTCTGGTGCC 
ATCTATCTGG CCTCGTCCTA CCAGAACAAA CTGCGGGTGA TCTGCTGTAA GGGCAGTCTG 
GTGCGGGAGT CTGGAGAGCT GCAGAGGACC GGCTCCAGCA GGAGCAGCCC CAACAAGCGC 
GGTCCACCAA CATACAACGA GCACATCTCC AAACGTCTGG CCTCGAGTCC TGGAGCCCAG 
GAGGGGCCCC TGCACCGCGA GCCCAGCACT CCTCACCGCT ACCGCGAGGG CCGCACCGAG 
TTCCGGAGAG ACAAATCTCC AGCCCGGCCC CTGGAGCGAG AGAAATCCCC GGGCCGTCCG 
GTGCTAGACA GCCGCAGAGA GAGGTCTCCC GGTCGCTTCG AGAGCAGCGG CAGCGAGAGA 
GACCGCGAGC GGACACGACT GCACACTAGC TCTGTACGCA CCCAGCTGAC CCCCGTCAAC 
AAGGTTTGGG ACCAGTCTTC GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100333880 ( XM_009303721.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301996.2 Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X5, mRNA.

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CloneID	ODa60008
Clone ID Related Accession (Same CDS sequence)	XM_009303721.3 , XM_009303721.2
Accession Version	XM_009303721.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	citron Rho-interacting kinase-like isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303721.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG AGATTTCCAA AGTGCAGCGT AAGATGTCAG ACTTGGAGTC AGTTTTGCAG 
CAGAAAGACG TGGAGCTCAA GGCTTCAGAA ACCCAGAGAA ACATCTTAGA GCAAGACCTG 
GCAACCTATA TCACCGAATG CAGCAGTTTG AAGCGCAGTT TGGAGCAGGC CCGCATGGAG 
GTTTCGCAGG AAGATGATAA AGCTCTTCAG CTGCTGCACG ACATTCGAGA GCAGAGCAAC 
AAACTGCAGG AAATCAAAGA ACAGGAGTAC CACGCTCAGC TGGAGGAGAT GCGAGTGTCT 
ATTCGTCAGC TGGAAGAGAA TCTGTCGGCC GCTCGTCGCC GCAGTGACCT GTACGAGTCT 
GAGCTCAAAG AGTCCCGTCA GACCAGTGAG GAGCTCAAGA GGAAGGCAGT GGAGTATCAG 
CAGAAGATCC AGAAGGCTAA AGAACAAGGG AAAGTTGAGA TGGAGGAGCT TCTGAGCAAA 
CTGCAGAAGA CCAATGGAGA GCAACAAGTA AAGATTGAAG AGCTCCAGGA GAAGCTTGCT 
AAGGCAGTGA AGGCGAGTAC TGAAGCCACT GAACTCCTCC AGAATGTCCG TCAGGCTAAA 
GAGCGAATGG AGCGAGACCT GGAGCGTCTG CAGACCAAAG AGGACTCGTC CGACAGCCTG 
CGCAGACGCT TACGAGAGAC TGAGGATGGC AAAAAATCCC TGGAGAACCA GGTGAAGAGG 
CTGGAGATGG TGGAGCGGAG AGAATCCAAA CTGAAAGACG ACATTCAGAC CAAATCGCAG 
CAGATCCAGC AGATGGCCGA CAAAATCATG GAGCTTGAAG AAAATTTGCG GGACTCGCAG 
GCGATGGCGC AGCGCATGGA AACACAGCTG GAGCAGAAGG AAAAGCTTTT TGAAGACAAG 
ATCAAGGTTT TAGAAACTCA AATGAAGGCT GATTTGGCTG AGAAGGATTT GCTGGAGTCC 
AAACAGAGCA CTTTTGAAGA GGAGTCTCGC GAACGGGGAA AAGTGATCAG CGACCAGGCA 
GCGACGATCA ATGCCATGGA GTCAAAGATG ACCAGTCTGG AGCAGAGGAT CGCTGAGCTG 
TCCGAAGCCA ACAAACTGGC AGCAAACAGC AGCATTTACA CACAGAAGAA CATGAAAGCC 
CAGGAGGAGA TGATTTCTGA ACTCAGACAG CAGAAGTTTT ATTTGGAGTC TCAGGCTGGA 
AAACTGGAGG CGCAGAATGC AAAGCTGGAG GAGCATCTGG AGAAACTCAC CCAACAAGAG 
CAGACAAACA AGAGCCGAGT GCTGGAGCTG GAAGCCAGGC TGCGAGAGAT CGGCCTAGAG 
CATGAGGAAC AGAAGCTGGA GATCAAGCGT CAGGTGTCGG AGCTCACGTT GTCTCTGCAG 
GAGAGAGAAG CTCAGATCAG CAGCCTGCAA GCGGCACGCC ACGCTCTCGA CAGCCAACTA 
CAGCAGGCCA AAACTGAGCT GGAGGAGACC ACCGCCGAGG CCGAAGAGGA GATCACCGTC 
CTGAGGGCCC ATCGGGATGA AATCCAACGC AAGTTTGATG CCCTGCGAGA CAGCTGTGCG 
GTGATCACGG ATCTGGAGGA GCAGTTGACG CAGCTCACGC AAGAAAACGC GGAGCTGAAC 
CGGCAGAACT TCTACCTGTC CAAACAGCTG GACGAGGCCA CAGACGAGAA CGAGGAGCGC 
CTGCAGCTCA AGCAGGACGT GGAGCGCCTC AGGAGAGAGG TGGCAGACCG AGAGATGCAC 
CTCAACAACC AGAAACAGGT GACACGGCAA CACACACACA CACACACACA CACACACACA 
CACAGGACAG AGGTGGCAGA CCGAGAGATG CACCTCAACA ACCAGAAACA GAACATCGAG 
ACGCTGAAGA CGACATGTAC AATGCTGGAG GAGCAGGTTA TGGAGCTGGA GACTCTGAAT 
GATGAGCTGA TGGAGAAGGA GAGGCAGTGG GAGAACTGGA GATCTGCTCT GGACGAGGAG 
AAAAACCAGG CCGAGCGCAG AGCCAGAGAC CTGCAAAGAC AGCTGGACAG CGAGAAGCAG 
AACCGACTGC GTGCAGAACA GAGAAACTCT GAGTCTCGGC AGGCCGTGGA GCTGGCGGTG 
AAAGAGCACA AAGCTGAAAT CCTGGTTCTG CAGCAGGCGC TCAAAGACCA GAAACTCAAG 
GCTGAGGGCC TTTCTGACAC TCTGGGTGAT CTGGAGAAGA AGCACGGCAT GCTGGAGATG 
AACGCTCGCA GCCTGCAGCA GAAGCTGGAG ACGGAGAGAG AGATGAAGCA GAGACTCATG 
GACGAGCAAG CTAAACTGCA GCAGCAGCTG GACATGCAGA AGAGCCACAT CTTCAGACTG 
ACTCAAGGAC TGCAGGATGC GCTGGACCAG ACCGACCTGC TGAAGACCGA GCGCACAGAC 
CTGGAGTACC AGCTGGAAAA CATACAGGCG GCGTATTCTC ACGAGAAGGT GAAGATGGAG 
GGGACGATCA CCCAGCAGAC CAAGCTCATA GACTTCCTGC AGGCCAAGGT AGAGCACCCC 
TCTAAGAAGA AAAAGGGGCT GTTTGGTGGC CGGCGGCGGG AGGATCTGCT AGCGGCGCTG 
GCGGCTCAGG GACAGACACA GGTGCCTGTG GTGCCCGCCG CCACTCCTGT GCCCCTGCAG 
TACAGCGACC TGAAGGCGGC GCTGGATAAA GAGCGAGCCC GATGCTCCGA GCTGGAGGAC 
GCCCTGCAGA AAACACGCCT GGAGCTCAGA GAAGTTCAGT TTAATAAAGC TCTGGAGCAC 
AGCTCTTCTC CAGCCACTCC AGGTTTGGCC CGTCAGCAGC TCATGATGTC CACCAAGTCC 
AACAAGTCTC CAGAGCACCA GTCCAGCGGC GGCCTGATGT CCTCAAACTC CAGCCGCGGA 
CGCAAAGACA ACGCCAATCC TGAAGAGTAC ACCAGACGTG TGAAGGACCG GATTCATCAC 
AACACTCCTC ACCGCTTCAC GCAGGGGCTC AATATGAGAG CGGCTCGCTG TACTGTGTGT 
CTGGACACAG TCCATTTTGG ACGTCAGGCT GCAACATGCA TTGAATGTCA CGCGTTGTGT 
CACCCAAAAT GCTCCCCGTA CCTCCCTGCC ACCTGCGGCG TTCCCACTGA ATACGCGCTG 
CACCTCTCAG AGGCTCTGTG TCGGGAGAAG GGAAGCACTT CCGGCCTGAA AGATATGGGT 
GGACATATGA GGCTTGAAGG ATGGCTGAAG CAGCCCAGGA ACGGGAAGCG CGGGCAGGGC 
TGGGAGAGGA AGTATGTGGT GCTGGATGGA TATAAAGTGA TTACATTCGA GACAGAACCA 
AGAGACGAGT CAGTGAAGCC GCTGGATGAG TTTGAGCTGT GTCTCTCTGA TGGAGAAGTG 
ACCGTTCATT CTGCTGTGGG TTCCTCTGAG CTCCCCAACA CCGCTAAAAC AGATGTGCCG 
TACGTGCTGA AGCTGGAGTC GTCTCCTCAG TCCACATGCT GGCCCGGTCA GACTCTGTAC 
CTCATGACCT CGGGATACTC AGAGAAGCAG CGCTGGGTGG CAGTGCTGGA AGCCATCGTG 
GCCAGCAGTC GCGGAGCCAG AGAGAAAGCT GAGGCAGATG CTAAATTATT GGGTAACTCT 
TTACTTAAGC TAGAAGGTGA CGACCGTCTG GATATCAACT GCACCCTGCC GCTGACCGAC 
CAGATTGTGT TGGTGGGCTC TGAGGAGGGT CTCTACGCAC TGAATGTGAT TAAGAACTCC 
CTCACTCATA TCCCGGGTCT GGGATCCGTG TTTCAGATCC ACATTATCAA AGATCATGAC 
AAGCTGCTGA TGATCGTGGG AGACGAGCGA GCGCTGTGTC TGGTGGAGAT TAAGAAGGTG 
AAGCAGTCTT TATCCCAGTC TCACCTCCCC ACACTGCCCG AGATTGTGCC GTATATCTTT 
GAGACCGTCA AGGGCTGTCA TCTGTTTGCC TCGGGCAGGA TAGAAAATGG CCCATGCATT 
TGTGCTGCGA TGCCCAATAA AGTCACGATT CTACGATACA ATGACAATCT TAACAAGTTC 
TGCATACGGA AGGAGATTGA AACTCTGGAG CCGTGCAGCT GCATCCACTT CACCAGCTAC 
AGCATCATCA TTGGTACCAA TAAATTCTAC GAGATCGAGA TGAAGCAGTG CGTTCTGGAC 
GAGTTCCTGG ACAAGAATGA CGTGTCGCTG GCGTCGGCTG TCTTCACCGG ATCCTCCCAC 
AGTTTCCCCA TCTCTATCGT CCAGGTGTCC AGCACTCCGC AGAAGGAGGA ATATCTGCTG 
TGTTTCCACG AGATCGGAGT TTTTGTGGAT GCTTACGGTC GAAGAAGTCG CCCTGATGAT 
CTGAAGTGGA GTCGACTTCC CCTGACGTTC GCCTTCAGAG AGCCGTATCT CTTTGTGACC 
TACTTCAACT CTCTGGATGT TATTGAGATT CAGGGACACG CAGCTCTTGG GCCTCCCAGT 
CTGGTTCACC TGGACATTCC TAACCCACGT TATCTGGGTC CAGCCATATC GTCTGGTGCC 
ATCTATCTGG CCTCGTCCTA CCAGAACAAA CTGCGGGTGA TCTGCTGTAA GGGCAGTCTG 
GTGCGGGAGT CTGGAGAGCT GCAGAGGACC GGCTCCAGCA GGAGCAGCCC CAACAAGCGC 
GGTCCACCAA CATACAACGA GCACATCTCC AAACGTCTGG CCTCGAGTCC TGGAGCCCAG 
GAGGGGCCCC TGCACCGCGA GCCCAGCACT CCTCACCGCT ACCGCGAGGG CCGCACCGAG 
TTCCGGAGAG ACAAATCTCC AGCCCGGCCC CTGGAGCGAG AGAAATCCCC GGGCCGTCCG 
GTGCTAGACA GCCGCAGAGA GAGGTCTCCC GGTCGCTTCG AGAGCAGCGG CAGCGAGAGA 
GACCGCGAGC GGACACGACT GCACACTAGC TCTGTACGCA CCCAGCTGAC CCCCGTCAAC 
AAGGTTTGGG ACCAGTCTTC GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301996.2
CDS	434..5380
Translation	MEQEISKVQRKMSDLESVLQQKDVELKASETQRNILEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSNKLQEIKEQEYHAQLEEMRVSIRQLEENLSAARRRSDLYESELKESRQTSEELKRKAVEYQQKIQKAKEQGKVEMEELLSKLQKTNGEQQVKIEELQEKLAKAVKASTEATELLQNVRQAKERMERDLERLQTKEDSSDSLRRRLRETEDGKKSLENQVKRLEMVERRESKLKDDIQTKSQQIQQMADKIMELEENLRDSQAMAQRMETQLEQKEKLFEDKIKVLETQMKADLAEKDLLESKQSTFEEESRERGKVISDQAATINAMESKMTSLEQRIAELSEANKLAANSSIYTQKNMKAQEEMISELRQQKFYLESQAGKLEAQNAKLEEHLEKLTQQEQTNKSRVLELEARLREIGLEHEEQKLEIKRQVSELTLSLQEREAQISSLQAARHALDSQLQQAKTELEETTAEAEEEITVLRAHRDEIQRKFDALRDSCAVITDLEEQLTQLTQENAELNRQNFYLSKQLDEATDENEERLQLKQDVERLRREVADREMHLNNQKQVTRQHTHTHTHTHTHRTEVADREMHLNNQKQNIETLKTTCTMLEEQVMELETLNDELMEKERQWENWRSALDEEKNQAERRARDLQRQLDSEKQNRLRAEQRNSESRQAVELAVKEHKAEILVLQQALKDQKLKAEGLSDTLGDLEKKHGMLEMNARSLQQKLETEREMKQRLMDEQAKLQQQLDMQKSHIFRLTQGLQDALDQTDLLKTERTDLEYQLENIQAAYSHEKVKMEGTITQQTKLIDFLQAKVEHPSKKKKGLFGGRRREDLLAALAAQGQTQVPVVPAATPVPLQYSDLKAALDKERARCSELEDALQKTRLELREVQFNKALEHSSSPATPGLARQQLMMSTKSNKSPEHQSSGGLMSSNSSRGRKDNANPEEYTRRVKDRIHHNTPHRFTQGLNMRAARCTVCLDTVHFGRQAATCIECHALCHPKCSPYLPATCGVPTEYALHLSEALCREKGSTSGLKDMGGHMRLEGWLKQPRNGKRGQGWERKYVVLDGYKVITFETEPRDESVKPLDEFELCLSDGEVTVHSAVGSSELPNTAKTDVPYVLKLESSPQSTCWPGQTLYLMTSGYSEKQRWVAVLEAIVASSRGAREKAEADAKLLGNSLLKLEGDDRLDINCTLPLTDQIVLVGSEEGLYALNVIKNSLTHIPGLGSVFQIHIIKDHDKLLMIVGDERALCLVEIKKVKQSLSQSHLPTLPEIVPYIFETVKGCHLFASGRIENGPCICAAMPNKVTILRYNDNLNKFCIRKEIETLEPCSCIHFTSYSIIIGTNKFYEIEMKQCVLDEFLDKNDVSLASAVFTGSSHSFPISIVQVSSTPQKEEYLLCFHEIGVFVDAYGRRSRPDDLKWSRLPLTFAFREPYLFVTYFNSLDVIEIQGHAALGPPSLVHLDIPNPRYLGPAISSGAIYLASSYQNKLRVICCKGSLVRESGELQRTGSSRSSPNKRGPPTYNEHISKRLASSPGAQEGPLHREPSTPHRYREGRTEFRRDKSPARPLEREKSPGRPVLDSRRERSPGRFESSGSERDRERTRLHTSSVRTQLTPVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_009303721.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303721.2

ORF Insert Sequence:

ATGGAGCAGG AGATTTCCAA AGTGCAGCGT AAGATGTCAG ACTTGGAGTC AGTTTTGCAG 
CAGAAAGACG TGGAGCTCAA GGCTTCAGAA ACCCAGAGAA ACATCTTAGA GCAAGACCTG 
GCAACCTATA TCACCGAATG CAGCAGTTTG AAGCGCAGTT TGGAGCAGGC CCGCATGGAG 
GTTTCGCAGG AAGATGATAA AGCTCTTCAG CTGCTGCACG ACATTCGAGA GCAGAGCAAC 
AAACTGCAGG AAATCAAAGA ACAGGAGTAC CACGCTCAGC TGGAGGAGAT GCGAGTGTCT 
ATTCGTCAGC TGGAAGAGAA TCTGTCGGCC GCTCGTCGCC GCAGTGACCT GTACGAGTCT 
GAGCTCAAAG AGTCCCGTCA GACCAGTGAG GAGCTCAAGA GGAAGGCAGT GGAGTATCAG 
CAGAAGATCC AGAAGGCTAA AGAACAAGGG AAAGTTGAGA TGGAGGAGCT TCTGAGCAAA 
CTGCAGAAGA CCAATGGAGA GCAACAAGTA AAGATTGAAG AGCTCCAGGA GAAGCTTGCT 
AAGGCAGTGA AGGCGAGTAC TGAAGCCACT GAACTCCTCC AGAATGTCCG TCAGGCTAAA 
GAGCGAATGG AGCGAGACCT GGAGCGTCTG CAGACCAAAG AGGACTCGTC CGACAGCCTG 
CGCAGACGCT TACGAGAGAC TGAGGATGGC AAAAAATCCC TGGAGAACCA GGTGAAGAGG 
CTGGAGATGG TGGAGCGGAG AGAATCCAAA CTGAAAGACG ACATTCAGAC CAAATCGCAG 
CAGATCCAGC AGATGGCCGA CAAAATCATG GAGCTTGAAG AAAATTTGCG GGACTCGCAG 
GCGATGGCGC AGCGCATGGA AACACAGCTG GAGCAGAAGG AAAAGCTTTT TGAAGACAAG 
ATCAAGGTTT TAGAAACTCA AATGAAGGCT GATTTGGCTG AGAAGGATTT GCTGGAGTCC 
AAACAGAGCA CTTTTGAAGA GGAGTCTCGC GAACGGGGAA AAGTGATCAG CGACCAGGCA 
GCGACGATCA ATGCCATGGA GTCAAAGATG ACCAGTCTGG AGCAGAGGAT CGCTGAGCTG 
TCCGAAGCCA ACAAACTGGC AGCAAACAGC AGCATTTACA CACAGAAGAA CATGAAAGCC 
CAGGAGGAGA TGATTTCTGA ACTCAGACAG CAGAAGTTTT ATTTGGAGTC TCAGGCTGGA 
AAACTGGAGG CGCAGAATGC AAAGCTGGAG GAGCATCTGG AGAAACTCAC CCAACAAGAG 
CAGACAAACA AGAGCCGAGT GCTGGAGCTG GAAGCCAGGC TGCGAGAGAT CGGCCTAGAG 
CATGAGGAAC AGAAGCTGGA GATCAAGCGT CAGGTGTCGG AGCTCACGTT GTCTCTGCAG 
GAGAGAGAAG CTCAGATCAG CAGCCTGCAA GCGGCACGCC ACGCTCTCGA CAGCCAACTA 
CAGCAGGCCA AAACTGAGCT GGAGGAGACC ACCGCCGAGG CCGAAGAGGA GATCACCGTC 
CTGAGGGCCC ATCGGGATGA AATCCAACGC AAGTTTGATG CCCTGCGAGA CAGCTGTGCG 
GTGATCACGG ATCTGGAGGA GCAGTTGACG CAGCTCACGC AAGAAAACGC GGAGCTGAAC 
CGGCAGAACT TCTACCTGTC CAAACAGCTG GACGAGGCCA CAGACGAGAA CGAGGAGCGC 
CTGCAGCTCA AGCAGGACGT GGAGCGCCTC AGGAGAGAGG TGGCAGACCG AGAGATGCAC 
CTCAACAACC AGAAACAGGT GACACGGCAA CACACACACA CACACACACA CACACACACA 
CACAGGACAG AGGTGGCAGA CCGAGAGATG CACCTCAACA ACCAGAAACA GAACATCGAG 
ACGCTGAAGA CGACATGTAC AATGCTGGAG GAGCAGGTTA TGGAGCTGGA GACTCTGAAT 
GATGAGCTGA TGGAGAAGGA GAGGCAGTGG GAGAACTGGA GATCTGCTCT GGACGAGGAG 
AAAAACCAGG CCGAGCGCAG AGCCAGAGAC CTGCAAAGAC AGCTGGACAG CGAGAAGCAG 
AACCGACTGC GTGCAGAACA GAGAAACTCT GAGTCTCGGC AGGCCGTGGA GCTGGCGGTG 
AAAGAGCACA AAGCTGAAAT CCTGGTTCTG CAGCAGGCGC TCAAAGACCA GAAACTCAAG 
GCTGAGGGCC TTTCTGACAC TCTGGGTGAT CTGGAGAAGA AGCACGGCAT GCTGGAGATG 
AACGCTCGCA GCCTGCAGCA GAAGCTGGAG ACGGAGAGAG AGATGAAGCA GAGACTCATG 
GACGAGCAAG CTAAACTGCA GCAGCAGCTG GACATGCAGA AGAGCCACAT CTTCAGACTG 
ACTCAAGGAC TGCAGGATGC GCTGGACCAG ACCGACCTGC TGAAGACCGA GCGCACAGAC 
CTGGAGTACC AGCTGGAAAA CATACAGGCG GCGTATTCTC ACGAGAAGGT GAAGATGGAG 
GGGACGATCA CCCAGCAGAC CAAGCTCATA GACTTCCTGC AGGCCAAGGT AGAGCACCCC 
TCTAAGAAGA AAAAGGGGCT GTTTGGTGGC CGGCGGCGGG AGGATCTGCT AGCGGCGCTG 
GCGGCTCAGG GACAGACACA GGTGCCTGTG GTGCCCGCCG CCACTCCTGT GCCCCTGCAG 
TACAGCGACC TGAAGGCGGC GCTGGATAAA GAGCGAGCCC GATGCTCCGA GCTGGAGGAC 
GCCCTGCAGA AAACACGCCT GGAGCTCAGA GAAGTTCAGT TTAATAAAGC TCTGGAGCAC 
AGCTCTTCTC CAGCCACTCC AGGTTTGGCC CGTCAGCAGC TCATGATGTC CACCAAGTCC 
AACAAGTCTC CAGAGCACCA GTCCAGCGGC GGCCTGATGT CCTCAAACTC CAGCCGCGGA 
CGCAAAGACA ACGCCAATCC TGAAGAGTAC ACCAGACGTG TGAAGGACCG GATTCATCAC 
AACACTCCTC ACCGCTTCAC GCAGGGGCTC AATATGAGAG CGGCTCGCTG TACTGTGTGT 
CTGGACACAG TCCATTTTGG ACGTCAGGCT GCAACATGCA TTGAATGTCA CGCGTTGTGT 
CACCCAAAAT GCTCCCCGTA CCTCCCTGCC ACCTGCGGCG TTCCCACTGA ATACGCGCTG 
CACCTCTCAG AGGCTCTGTG TCGGGAGAAG GGAAGCACTT CCGGCCTGAA AGATATGGGT 
GGACATATGA GGCTTGAAGG ATGGCTGAAG CAGCCCAGGA ACGGGAAGCG CGGGCAGGGC 
TGGGAGAGGA AGTATGTGGT GCTGGATGGA TATAAAGTGA TTACATTCGA GACAGAACCA 
AGAGACGAGT CAGTGAAGCC GCTGGATGAG TTTGAGCTGT GTCTCTCTGA TGGAGAAGTG 
ACCGTTCATT CTGCTGTGGG TTCCTCTGAG CTCCCCAACA CCGCTAAAAC AGATGTGCCG 
TACGTGCTGA AGCTGGAGTC GTCTCCTCAG TCCACATGCT GGCCCGGTCA GACTCTGTAC 
CTCATGACCT CGGGATACTC AGAGAAGCAG CGCTGGGTGG CAGTGCTGGA AGCCATCGTG 
GCCAGCAGTC GCGGAGCCAG AGAGAAAGCT GAGGCAGATG CTAAATTATT GGGTAACTCT 
TTACTTAAGC TAGAAGGTGA CGACCGTCTG GATATCAACT GCACCCTGCC GCTGACCGAC 
CAGATTGTGT TGGTGGGCTC TGAGGAGGGT CTCTACGCAC TGAATGTGAT TAAGAACTCC 
CTCACTCATA TCCCGGGTCT GGGATCCGTG TTTCAGATCC ACATTATCAA AGATCATGAC 
AAGCTGCTGA TGATCGTGGG AGACGAGCGA GCGCTGTGTC TGGTGGAGAT TAAGAAGGTG 
AAGCAGTCTT TATCCCAGTC TCACCTCCCC ACACTGCCCG AGATTGTGCC GTATATCTTT 
GAGACCGTCA AGGGCTGTCA TCTGTTTGCC TCGGGCAGGA TAGAAAATGG CCCATGCATT 
TGTGCTGCGA TGCCCAATAA AGTCACGATT CTACGATACA ATGACAATCT TAACAAGTTC 
TGCATACGGA AGGAGATTGA AACTCTGGAG CCGTGCAGCT GCATCCACTT CACCAGCTAC 
AGCATCATCA TTGGTACCAA TAAATTCTAC GAGATCGAGA TGAAGCAGTG CGTTCTGGAC 
GAGTTCCTGG ACAAGAATGA CGTGTCGCTG GCGTCGGCTG TCTTCACCGG ATCCTCCCAC 
AGTTTCCCCA TCTCTATCGT CCAGGTGTCC AGCACTCCGC AGAAGGAGGA ATATCTGCTG 
TGTTTCCACG AGATCGGAGT TTTTGTGGAT GCTTACGGTC GAAGAAGTCG CCCTGATGAT 
CTGAAGTGGA GTCGACTTCC CCTGACGTTC GCCTTCAGAG AGCCGTATCT CTTTGTGACC 
TACTTCAACT CTCTGGATGT TATTGAGATT CAGGGACACG CAGCTCTTGG GCCTCCCAGT 
CTGGTTCACC TGGACATTCC TAACCCACGT TATCTGGGTC CAGCCATATC GTCTGGTGCC 
ATCTATCTGG CCTCGTCCTA CCAGAACAAA CTGCGGGTGA TCTGCTGTAA GGGCAGTCTG 
GTGCGGGAGT CTGGAGAGCT GCAGAGGACC GGCTCCAGCA GGAGCAGCCC CAACAAGCGC 
GGTCCACCAA CATACAACGA GCACATCTCC AAACGTCTGG CCTCGAGTCC TGGAGCCCAG 
GAGGGGCCCC TGCACCGCGA GCCCAGCACT CCTCACCGCT ACCGCGAGGG CCGCACCGAG 
TTCCGGAGAG ACAAATCTCC AGCCCGGCCC CTGGAGCGAG AGAAATCCCC GGGCCGTCCG 
GTGCTAGACA GCCGCAGAGA GAGGTCTCCC GGTCGCTTCG AGAGCAGCGG CAGCGAGAGA 
GACCGCGAGC GGACACGACT GCACACTAGC TCTGTACGCA CCCAGCTGAC CCCCGTCAAC 
AAGGTTTGGG ACCAGTCTTC GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100333880 ( XM_009303722.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301997.2 Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X6, mRNA.

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CloneID	ODa60009
Clone ID Related Accession (Same CDS sequence)	XM_009303722.2
Accession Version	XM_009303722.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	citron Rho-interacting kinase-like isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303722.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG AGATTTCCAA AGTGCAGCGT AAGATGTCAG ACTTGGAGTC AGTTTTGCAG 
CAGAAAGACG TGGAGCTCAA GGCTTCAGAA ACCCAGAGAA ACATCTTAGA GCAAGACCTG 
GCAACCTATA TCACCGAATG CAGCAGTTTG AAGCGCAGTT TGGAGCAGGC CCGCATGGAG 
GTTTCGCAGG AAGATGATAA AGCTCTTCAG CTGCTGCACG ACATTCGAGA GCAGAGCAAC 
AAACTGCAGG AAATCAAAGA ACAGGAGTAC CACGCTCAGC TGGAGGAGAT GCGAGTGTCT 
ATTCGTCAGC TGGAAGAGAA TCTGTCGGCC GCTCGTCGCC GCAGTGACCT GTACGAGTCT 
GAGCTCAAAG AGTCCCGTCA GACCAGTGAG GAGCTCAAGA GGAAGGCAGT GGAGTATCAG 
CAGAAGATCC AGAAGGCTAA AGAACAAGGG AAAGTTGAGA TGGAGGAGCT TCTGAGCAAA 
CTGCAGAAGA CCAATGGAGA GCAACAAGTA AAGATTGAAG AGCTCCAGGA GAAGCTTGCT 
AAGGCAGTGA AGGCGAGTAC TGAAGCCACT GAACTCCTCC AGAATGTCCG TCAGGCTAAA 
GAGCGAATGG AGCGAGACCT GGAGCGTCTG CAGACCAAAG AGGACTCGTC CGACAGCCTG 
CGCAGACGCT TACGAGAGAC TGAGGATGGC AAAAAATCCC TGGAGAACCA GGTGAAGAGG 
CTGGAGATGG TGGAGCGGAG AGAATCCAAA CTGAAAGACG ACATTCAGAC CAAATCGCAG 
CAGATCCAGC AGATGGCCGA CAAAATCATG GAGCTTGAAG AAAATTTGCG GGACTCGCAG 
GCGATGGCGC AGCGCATGGA AACACAGCTG GAGCAGAAGG AAAAGCTTTT TGAAGACAAG 
ATCAAGGTTT TAGAAACTCA AATGAAGGCT GATTTGGCTG AGAAGGATTT GCTGGAGTCC 
AAACAGAGCA CTTTTGAAGA GGAGTCTCGC GAACGGGGAA AAGTGATCAG CGACCAGGCA 
GCGACGATCA ATGCCATGGA GTCAAAGATG ACCAGTCTGG AGCAGAGGAT CGCTGAGCTG 
TCCGAAGCCA ACAAACTGGC AGCAAACAGC AGCATTTACA CACAGAAGAA CATGAAAGCC 
CAGGAGGAGA TGATTTCTGA ACTCAGACAG CAGAAGTTTT ATTTGGAGTC TCAGGCTGGA 
AAACTGGAGG CGCAGAATGC AAAGCTGGAG GAGCATCTGG AGAAACTCAC CCAACAAGAG 
CAGACAAACA AGAGCCGAGT GCTGGAGCTG GAAGCCAGGC TGCGAGAGAT CGGCCTAGAG 
CATGAGGAAC AGAAGCTGGA GATCAAGCGT CAGGTGTCGG AGCTCACGTT GTCTCTGCAG 
GAGAGAGAAG CTCAGATCAG CAGCCTGCAA GCGGCACGCC ACGCTCTCGA CAGCCAACTA 
CAGCAGGCCA AAACTGAGCT GGAGGAGACC ACCGCCGAGG CCGAAGAGGA GATCACCGTC 
CTGAGGGCCC ATCGGGATGA AATCCAACGC AAGTTTGATG CCCTGCGAGA CAGCTGTGCG 
GTGATCACGG ATCTGGAGGA GCAGTTGACG CAGCTCACGC AAGAAAACGC GGAGCTGAAC 
CGGCAGAACT TCTACCTGTC CAAACAGCTG GACGAGGCCA CAGACGAGAA CGAGGAGCGC 
CTGCAGCTCA AGCAGGACGT GGAGCGCCTC AGGAGAGAGG TGGCAGACCG AGAGATGCAC 
CTCAACAACC AGAAACAGGT GACACGGCAA CACACACACA CACACACACA CACACACACA 
CACAGGACAG AGGTGGCAGA CCGAGAGATG CACCTCAACA ACCAGAAACA GAACATCGAG 
ACGCTGAAGA CGACATGTAC AATGCTGGAG GAGCAGGTTA TGGAGCTGGA GACTCTGAAT 
GATGAGCTGA TGGAGAAGGA GAGGCAGTGG GAGAACTGGA GATCTGCTCT GGACGAGGAG 
AAAAACCAGG CCGAGCGCAG AGCCAGAGAC CTGCAAAGAC AGCTGGACAG CGAGAAGCAG 
AACCGACTGC GTGCAGAACA GAGAAACTCT GAGTCTCGGC AGGCCGTGGA GCTGGCGGTG 
AAAGAGCACA AAGCTGAAAT CCTGGTTCTG CAGCAGGCGC TCAAAGACCA GAAACTCAAG 
GCTGAGGGCC TTTCTGACAC TCTGGGTGAT CTGGAGAAGA AGCACGGCAT GCTGGAGATG 
AACGCTCGCA GCCTGCAGCA GAAGCTGGAG ACGGAGAGAG AGATGAAGCA GAGACTCATG 
GACGAGCAAG CTAAACTGCA GCAGCAGCTG GACATGCAGA AGAGCCACAT CTTCAGACTG 
ACTCAAGGAC TGCAGGATGC GCTGGACCAG ACCGACCTGC TGAAGACCGA GCGCACAGAC 
CTGGAGTACC AGCTGGAAAA CATACAGGCG GCGTATTCTC ACGAGAAGGT GAAGATGGAG 
GGGACGATCA CCCAGCAGAC CAAGCTCATA GACTTCCTGC AGGCCAAGGG GCTGTTTGGT 
GGCCGGCGGC GGGAGGATCT GCTAGCGGCG CTGGCGGCTC AGGGACAGAC ACAGGTGCCT 
GTGGTGCCCG CCGCCACTCC TGTGCCCCTG CAGTACAGCG ACCTGAAGGC GGCGCTGGAT 
AAAGAGCGAG CCCGATGCTC CGAGCTGGAG GACGCCCTGC AGAAAACACG CCTGGAGCTC 
AGAGAAGTTC AGTTTAATAA AGCTCTGGAG CACAGCTCTT CTCCAGCCAC TCCAGGTTTG 
GCCCGTCAGC AGCTCATGAT GTCCACCAAG TCCAACAAGT CTCCAGAGCA CCAGTCCAGC 
GGCGGCCTGA TGTCCTCAAA CTCCAGCCGC GGACGCAAAG ACAACGCCAA TCCTGAAGAA 
ATAAGGAGAG TCACTTCTGA AAAGTACACC AGACGTGTGA AGGACCGGAT TCATCACAAC 
ACTCCTCACC GCTTCACGCA GGGGCTCAAT ATGAGAGCGG CTCGCTGTAC TGTGTGTCTG 
GACACAGTCC ATTTTGGACG TCAGGCTGCA ACATGCATTG AATGTCACGC GTTGTGTCAC 
CCAAAATGCT CCCCGTACCT CCCTGCCACC TGCGGCGTTC CCACTGAATA CGCGCTGCAC 
CTCTCAGAGG CTCTGTGTCG GGAGAAGGGA AGCACTTCCG GCCTGAAAGA TATGGGTGGA 
CATATGAGGC TTGAAGGATG GCTGAAGCAG CCCAGGAACG GGAAGCGCGG GCAGGGCTGG 
GAGAGGAAGT ATGTGGTGCT GGATGGATAT AAAGTGATTA CATTCGAGAC AGAACCAAGA 
GACGAGTCAG TGAAGCCGCT GGATGAGTTT GAGCTGTGTC TCTCTGATGG AGAAGTGACC 
GTTCATTCTG CTGTGGGTTC CTCTGAGCTC CCCAACACCG CTAAAACAGA TGTGCCGTAC 
GTGCTGAAGC TGGAGTCGTC TCCTCAGTCC ACATGCTGGC CCGGTCAGAC TCTGTACCTC 
ATGACCTCGG GATACTCAGA GAAGCAGCGC TGGGTGGCAG TGCTGGAAGC CATCGTGGCC 
AGCAGTCGCG GAGCCAGAGA GAAAGCTGAG GCAGATGCTA AATTATTGGG TAACTCTTTA 
CTTAAGCTAG AAGGTGACGA CCGTCTGGAT ATCAACTGCA CCCTGCCGCT GACCGACCAG 
ATTGTGTTGG TGGGCTCTGA GGAGGGTCTC TACGCACTGA ATGTGATTAA GAACTCCCTC 
ACTCATATCC CGGGTCTGGG ATCCGTGTTT CAGATCCACA TTATCAAAGA TCATGACAAG 
CTGCTGATGA TCGTGGGAGA CGAGCGAGCG CTGTGTCTGG TGGAGATTAA GAAGGTGAAG 
CAGTCTTTAT CCCAGTCTCA CCTCCCCACA CTGCCCGAGA TTGTGCCGTA TATCTTTGAG 
ACCGTCAAGG GCTGTCATCT GTTTGCCTCG GGCAGGATAG AAAATGGCCC ATGCATTTGT 
GCTGCGATGC CCAATAAAGT CACGATTCTA CGATACAATG ACAATCTTAA CAAGTTCTGC 
ATACGGAAGG AGATTGAAAC TCTGGAGCCG TGCAGCTGCA TCCACTTCAC CAGCTACAGC 
ATCATCATTG GTACCAATAA ATTCTACGAG ATCGAGATGA AGCAGTGCGT TCTGGACGAG 
TTCCTGGACA AGAATGACGT GTCGCTGGCG TCGGCTGTCT TCACCGGATC CTCCCACAGT 
TTCCCCATCT CTATCGTCCA GGTGTCCAGC ACTCCGCAGA AGGAGGAATA TCTGCTGTGT 
TTCCACGAGA TCGGAGTTTT TGTGGATGCT TACGGTCGAA GAAGTCGCCC TGATGATCTG 
AAGTGGAGTC GACTTCCCCT GACGTTCGCC TTCAGAGAGC CGTATCTCTT TGTGACCTAC 
TTCAACTCTC TGGATGTTAT TGAGATTCAG GGACACGCAG CTCTTGGGCC TCCCAGTCTG 
GTTCACCTGG ACATTCCTAA CCCACGTTAT CTGGGTCCAG CCATATCGTC TGGTGCCATC 
TATCTGGCCT CGTCCTACCA GAACAAACTG CGGGTGATCT GCTGTAAGGG CAGTCTGGTG 
CGGGAGTCTG GAGAGCTGCA GAGGACCGGC TCCAGCAGGA GCAGCCCCAA CAAGCGCGGT 
CCACCAACAT ACAACGAGCA CATCTCCAAA CGTCTGGCCT CGAGTCCTGG AGCCCAGGAG 
GGGCCCCTGC ACCGCGAGCC CAGCACTCCT CACCGCTACC GCGAGGGCCG CACCGAGTTC 
CGGAGAGACA AATCTCCAGC CCGGCCCCTG GAGCGAGAGA AATCCCCGGG CCGTCCGGTG 
CTAGACAGCC GCAGAGAGAG GTCTCCCGGT CGCTTCGAGA GCAGCGGCAG CGAGAGAGAC 
CGCGAGCGGA CACGACTGCA CACTAGCTCT GTACGCACCC AGCTGACCCC CGTCAACAAG 
GTTTGGGACC AGTCTTCGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301997.2
CDS	434..5377
Translation	MEQEISKVQRKMSDLESVLQQKDVELKASETQRNILEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSNKLQEIKEQEYHAQLEEMRVSIRQLEENLSAARRRSDLYESELKESRQTSEELKRKAVEYQQKIQKAKEQGKVEMEELLSKLQKTNGEQQVKIEELQEKLAKAVKASTEATELLQNVRQAKERMERDLERLQTKEDSSDSLRRRLRETEDGKKSLENQVKRLEMVERRESKLKDDIQTKSQQIQQMADKIMELEENLRDSQAMAQRMETQLEQKEKLFEDKIKVLETQMKADLAEKDLLESKQSTFEEESRERGKVISDQAATINAMESKMTSLEQRIAELSEANKLAANSSIYTQKNMKAQEEMISELRQQKFYLESQAGKLEAQNAKLEEHLEKLTQQEQTNKSRVLELEARLREIGLEHEEQKLEIKRQVSELTLSLQEREAQISSLQAARHALDSQLQQAKTELEETTAEAEEEITVLRAHRDEIQRKFDALRDSCAVITDLEEQLTQLTQENAELNRQNFYLSKQLDEATDENEERLQLKQDVERLRREVADREMHLNNQKQVTRQHTHTHTHTHTHRTEVADREMHLNNQKQNIETLKTTCTMLEEQVMELETLNDELMEKERQWENWRSALDEEKNQAERRARDLQRQLDSEKQNRLRAEQRNSESRQAVELAVKEHKAEILVLQQALKDQKLKAEGLSDTLGDLEKKHGMLEMNARSLQQKLETEREMKQRLMDEQAKLQQQLDMQKSHIFRLTQGLQDALDQTDLLKTERTDLEYQLENIQAAYSHEKVKMEGTITQQTKLIDFLQAKGLFGGRRREDLLAALAAQGQTQVPVVPAATPVPLQYSDLKAALDKERARCSELEDALQKTRLELREVQFNKALEHSSSPATPGLARQQLMMSTKSNKSPEHQSSGGLMSSNSSRGRKDNANPEEIRRVTSEKYTRRVKDRIHHNTPHRFTQGLNMRAARCTVCLDTVHFGRQAATCIECHALCHPKCSPYLPATCGVPTEYALHLSEALCREKGSTSGLKDMGGHMRLEGWLKQPRNGKRGQGWERKYVVLDGYKVITFETEPRDESVKPLDEFELCLSDGEVTVHSAVGSSELPNTAKTDVPYVLKLESSPQSTCWPGQTLYLMTSGYSEKQRWVAVLEAIVASSRGAREKAEADAKLLGNSLLKLEGDDRLDINCTLPLTDQIVLVGSEEGLYALNVIKNSLTHIPGLGSVFQIHIIKDHDKLLMIVGDERALCLVEIKKVKQSLSQSHLPTLPEIVPYIFETVKGCHLFASGRIENGPCICAAMPNKVTILRYNDNLNKFCIRKEIETLEPCSCIHFTSYSIIIGTNKFYEIEMKQCVLDEFLDKNDVSLASAVFTGSSHSFPISIVQVSSTPQKEEYLLCFHEIGVFVDAYGRRSRPDDLKWSRLPLTFAFREPYLFVTYFNSLDVIEIQGHAALGPPSLVHLDIPNPRYLGPAISSGAIYLASSYQNKLRVICCKGSLVRESGELQRTGSSRSSPNKRGPPTYNEHISKRLASSPGAQEGPLHREPSTPHRYREGRTEFRRDKSPARPLEREKSPGRPVLDSRRERSPGRFESSGSERDRERTRLHTSSVRTQLTPVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_009303722.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303722.2

ORF Insert Sequence:

ATGGAGCAGG AGATTTCCAA AGTGCAGCGT AAGATGTCAG ACTTGGAGTC AGTTTTGCAG 
CAGAAAGACG TGGAGCTCAA GGCTTCAGAA ACCCAGAGAA ACATCTTAGA GCAAGACCTG 
GCAACCTATA TCACCGAATG CAGCAGTTTG AAGCGCAGTT TGGAGCAGGC CCGCATGGAG 
GTTTCGCAGG AAGATGATAA AGCTCTTCAG CTGCTGCACG ACATTCGAGA GCAGAGCAAC 
AAACTGCAGG AAATCAAAGA ACAGGAGTAC CACGCTCAGC TGGAGGAGAT GCGAGTGTCT 
ATTCGTCAGC TGGAAGAGAA TCTGTCGGCC GCTCGTCGCC GCAGTGACCT GTACGAGTCT 
GAGCTCAAAG AGTCCCGTCA GACCAGTGAG GAGCTCAAGA GGAAGGCAGT GGAGTATCAG 
CAGAAGATCC AGAAGGCTAA AGAACAAGGG AAAGTTGAGA TGGAGGAGCT TCTGAGCAAA 
CTGCAGAAGA CCAATGGAGA GCAACAAGTA AAGATTGAAG AGCTCCAGGA GAAGCTTGCT 
AAGGCAGTGA AGGCGAGTAC TGAAGCCACT GAACTCCTCC AGAATGTCCG TCAGGCTAAA 
GAGCGAATGG AGCGAGACCT GGAGCGTCTG CAGACCAAAG AGGACTCGTC CGACAGCCTG 
CGCAGACGCT TACGAGAGAC TGAGGATGGC AAAAAATCCC TGGAGAACCA GGTGAAGAGG 
CTGGAGATGG TGGAGCGGAG AGAATCCAAA CTGAAAGACG ACATTCAGAC CAAATCGCAG 
CAGATCCAGC AGATGGCCGA CAAAATCATG GAGCTTGAAG AAAATTTGCG GGACTCGCAG 
GCGATGGCGC AGCGCATGGA AACACAGCTG GAGCAGAAGG AAAAGCTTTT TGAAGACAAG 
ATCAAGGTTT TAGAAACTCA AATGAAGGCT GATTTGGCTG AGAAGGATTT GCTGGAGTCC 
AAACAGAGCA CTTTTGAAGA GGAGTCTCGC GAACGGGGAA AAGTGATCAG CGACCAGGCA 
GCGACGATCA ATGCCATGGA GTCAAAGATG ACCAGTCTGG AGCAGAGGAT CGCTGAGCTG 
TCCGAAGCCA ACAAACTGGC AGCAAACAGC AGCATTTACA CACAGAAGAA CATGAAAGCC 
CAGGAGGAGA TGATTTCTGA ACTCAGACAG CAGAAGTTTT ATTTGGAGTC TCAGGCTGGA 
AAACTGGAGG CGCAGAATGC AAAGCTGGAG GAGCATCTGG AGAAACTCAC CCAACAAGAG 
CAGACAAACA AGAGCCGAGT GCTGGAGCTG GAAGCCAGGC TGCGAGAGAT CGGCCTAGAG 
CATGAGGAAC AGAAGCTGGA GATCAAGCGT CAGGTGTCGG AGCTCACGTT GTCTCTGCAG 
GAGAGAGAAG CTCAGATCAG CAGCCTGCAA GCGGCACGCC ACGCTCTCGA CAGCCAACTA 
CAGCAGGCCA AAACTGAGCT GGAGGAGACC ACCGCCGAGG CCGAAGAGGA GATCACCGTC 
CTGAGGGCCC ATCGGGATGA AATCCAACGC AAGTTTGATG CCCTGCGAGA CAGCTGTGCG 
GTGATCACGG ATCTGGAGGA GCAGTTGACG CAGCTCACGC AAGAAAACGC GGAGCTGAAC 
CGGCAGAACT TCTACCTGTC CAAACAGCTG GACGAGGCCA CAGACGAGAA CGAGGAGCGC 
CTGCAGCTCA AGCAGGACGT GGAGCGCCTC AGGAGAGAGG TGGCAGACCG AGAGATGCAC 
CTCAACAACC AGAAACAGGT GACACGGCAA CACACACACA CACACACACA CACACACACA 
CACAGGACAG AGGTGGCAGA CCGAGAGATG CACCTCAACA ACCAGAAACA GAACATCGAG 
ACGCTGAAGA CGACATGTAC AATGCTGGAG GAGCAGGTTA TGGAGCTGGA GACTCTGAAT 
GATGAGCTGA TGGAGAAGGA GAGGCAGTGG GAGAACTGGA GATCTGCTCT GGACGAGGAG 
AAAAACCAGG CCGAGCGCAG AGCCAGAGAC CTGCAAAGAC AGCTGGACAG CGAGAAGCAG 
AACCGACTGC GTGCAGAACA GAGAAACTCT GAGTCTCGGC AGGCCGTGGA GCTGGCGGTG 
AAAGAGCACA AAGCTGAAAT CCTGGTTCTG CAGCAGGCGC TCAAAGACCA GAAACTCAAG 
GCTGAGGGCC TTTCTGACAC TCTGGGTGAT CTGGAGAAGA AGCACGGCAT GCTGGAGATG 
AACGCTCGCA GCCTGCAGCA GAAGCTGGAG ACGGAGAGAG AGATGAAGCA GAGACTCATG 
GACGAGCAAG CTAAACTGCA GCAGCAGCTG GACATGCAGA AGAGCCACAT CTTCAGACTG 
ACTCAAGGAC TGCAGGATGC GCTGGACCAG ACCGACCTGC TGAAGACCGA GCGCACAGAC 
CTGGAGTACC AGCTGGAAAA CATACAGGCG GCGTATTCTC ACGAGAAGGT GAAGATGGAG 
GGGACGATCA CCCAGCAGAC CAAGCTCATA GACTTCCTGC AGGCCAAGGG GCTGTTTGGT 
GGCCGGCGGC GGGAGGATCT GCTAGCGGCG CTGGCGGCTC AGGGACAGAC ACAGGTGCCT 
GTGGTGCCCG CCGCCACTCC TGTGCCCCTG CAGTACAGCG ACCTGAAGGC GGCGCTGGAT 
AAAGAGCGAG CCCGATGCTC CGAGCTGGAG GACGCCCTGC AGAAAACACG CCTGGAGCTC 
AGAGAAGTTC AGTTTAATAA AGCTCTGGAG CACAGCTCTT CTCCAGCCAC TCCAGGTTTG 
GCCCGTCAGC AGCTCATGAT GTCCACCAAG TCCAACAAGT CTCCAGAGCA CCAGTCCAGC 
GGCGGCCTGA TGTCCTCAAA CTCCAGCCGC GGACGCAAAG ACAACGCCAA TCCTGAAGAA 
ATAAGGAGAG TCACTTCTGA AAAGTACACC AGACGTGTGA AGGACCGGAT TCATCACAAC 
ACTCCTCACC GCTTCACGCA GGGGCTCAAT ATGAGAGCGG CTCGCTGTAC TGTGTGTCTG 
GACACAGTCC ATTTTGGACG TCAGGCTGCA ACATGCATTG AATGTCACGC GTTGTGTCAC 
CCAAAATGCT CCCCGTACCT CCCTGCCACC TGCGGCGTTC CCACTGAATA CGCGCTGCAC 
CTCTCAGAGG CTCTGTGTCG GGAGAAGGGA AGCACTTCCG GCCTGAAAGA TATGGGTGGA 
CATATGAGGC TTGAAGGATG GCTGAAGCAG CCCAGGAACG GGAAGCGCGG GCAGGGCTGG 
GAGAGGAAGT ATGTGGTGCT GGATGGATAT AAAGTGATTA CATTCGAGAC AGAACCAAGA 
GACGAGTCAG TGAAGCCGCT GGATGAGTTT GAGCTGTGTC TCTCTGATGG AGAAGTGACC 
GTTCATTCTG CTGTGGGTTC CTCTGAGCTC CCCAACACCG CTAAAACAGA TGTGCCGTAC 
GTGCTGAAGC TGGAGTCGTC TCCTCAGTCC ACATGCTGGC CCGGTCAGAC TCTGTACCTC 
ATGACCTCGG GATACTCAGA GAAGCAGCGC TGGGTGGCAG TGCTGGAAGC CATCGTGGCC 
AGCAGTCGCG GAGCCAGAGA GAAAGCTGAG GCAGATGCTA AATTATTGGG TAACTCTTTA 
CTTAAGCTAG AAGGTGACGA CCGTCTGGAT ATCAACTGCA CCCTGCCGCT GACCGACCAG 
ATTGTGTTGG TGGGCTCTGA GGAGGGTCTC TACGCACTGA ATGTGATTAA GAACTCCCTC 
ACTCATATCC CGGGTCTGGG ATCCGTGTTT CAGATCCACA TTATCAAAGA TCATGACAAG 
CTGCTGATGA TCGTGGGAGA CGAGCGAGCG CTGTGTCTGG TGGAGATTAA GAAGGTGAAG 
CAGTCTTTAT CCCAGTCTCA CCTCCCCACA CTGCCCGAGA TTGTGCCGTA TATCTTTGAG 
ACCGTCAAGG GCTGTCATCT GTTTGCCTCG GGCAGGATAG AAAATGGCCC ATGCATTTGT 
GCTGCGATGC CCAATAAAGT CACGATTCTA CGATACAATG ACAATCTTAA CAAGTTCTGC 
ATACGGAAGG AGATTGAAAC TCTGGAGCCG TGCAGCTGCA TCCACTTCAC CAGCTACAGC 
ATCATCATTG GTACCAATAA ATTCTACGAG ATCGAGATGA AGCAGTGCGT TCTGGACGAG 
TTCCTGGACA AGAATGACGT GTCGCTGGCG TCGGCTGTCT TCACCGGATC CTCCCACAGT 
TTCCCCATCT CTATCGTCCA GGTGTCCAGC ACTCCGCAGA AGGAGGAATA TCTGCTGTGT 
TTCCACGAGA TCGGAGTTTT TGTGGATGCT TACGGTCGAA GAAGTCGCCC TGATGATCTG 
AAGTGGAGTC GACTTCCCCT GACGTTCGCC TTCAGAGAGC CGTATCTCTT TGTGACCTAC 
TTCAACTCTC TGGATGTTAT TGAGATTCAG GGACACGCAG CTCTTGGGCC TCCCAGTCTG 
GTTCACCTGG ACATTCCTAA CCCACGTTAT CTGGGTCCAG CCATATCGTC TGGTGCCATC 
TATCTGGCCT CGTCCTACCA GAACAAACTG CGGGTGATCT GCTGTAAGGG CAGTCTGGTG 
CGGGAGTCTG GAGAGCTGCA GAGGACCGGC TCCAGCAGGA GCAGCCCCAA CAAGCGCGGT 
CCACCAACAT ACAACGAGCA CATCTCCAAA CGTCTGGCCT CGAGTCCTGG AGCCCAGGAG 
GGGCCCCTGC ACCGCGAGCC CAGCACTCCT CACCGCTACC GCGAGGGCCG CACCGAGTTC 
CGGAGAGACA AATCTCCAGC CCGGCCCCTG GAGCGAGAGA AATCCCCGGG CCGTCCGGTG 
CTAGACAGCC GCAGAGAGAG GTCTCCCGGT CGCTTCGAGA GCAGCGGCAG CGAGAGAGAC 
CGCGAGCGGA CACGACTGCA CACTAGCTCT GTACGCACCC AGCTGACCCC CGTCAACAAG 
GTTTGGGACC AGTCTTCGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100333880 ( XM_017357404.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212893.1 Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X8, mRNA.

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CloneID	ODa60010
Clone ID Related Accession (Same CDS sequence)	XM_017357404.2 , XM_017357404.1
Accession Version	XM_017357404.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	citron Rho-interacting kinase-like isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017357404.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG AGATTTCCAA AGTGCAGCGT AAGATGTCAG ACTTGGAGTC AGTTTTGCAG 
CAGAAAGACG TGGAGCTCAA GGCTTCAGAA ACCCAGAGAA ACATCTTAGA GCAAGACCTG 
GCAACCTATA TCACCGAATG CAGCAGTTTG AAGCGCAGTT TGGAGCAGGC CCGCATGGAG 
GTTTCGCAGG AAGATGATAA AGCTCTTCAG CTGCTGCACG ACATTCGAGA GCAGAGCAAC 
AAACTGCAGG AAATCAAAGA ACAGGAGTAC CACGCTCAGC TGGAGGAGAT GCGAGTGTCT 
ATTCGTCAGC TGGAAGAGAA TCTGTCGGCC GCTCGTCGCC GCAGTGACCT GTACGAGTCT 
GAGCTCAAAG AGTCCCGTCA GACCAGTGAG GAGCTCAAGA GGAAGGCAGT GGAGTATCAG 
CAGAAGATCC AGAAGGCTAA AGAACAAGGG AAAGTTGAGA TGGAGGAGCT TCTGAGCAAA 
CTGCAGAAGA CCAATGGAGA GCAACAAGTA AAGATTGAAG AGCTCCAGGA GAAGCTTGCT 
AAGGCAGTGA AGGCGAGTAC TGAAGCCACT GAACTCCTCC AGAATGTCCG TCAGGCTAAA 
GAGCGAATGG AGCGAGACCT GGAGCGTCTG CAGACCAAAG AGGACTCGTC CGACAGCCTG 
CGCAGACGCT TACGAGAGAC TGAGGATGGC AAAAAATCCC TGGAGAACCA GGTGAAGAGG 
CTGGAGATGG TGGAGCGGAG AGAATCCAAA CTGAAAGACG ACATTCAGAC CAAATCGCAG 
CAGATCCAGC AGATGGCCGA CAAAATCATG GAGCTTGAAG AAAATTTGCG GGACTCGCAG 
GCGATGGCGC AGCGCATGGA AACACAGCTG GAGCAGAAGG AAAAGCTTTT TGAAGACAAG 
ATCAAGGTTT TAGAAACTCA AATGAAGGCT GATTTGGCTG AGAAGGATTT GCTGGAGTCC 
AAACAGAGCA CTTTTGAAGA GGAGTCTCGC GAACGGGGAA AAGTGATCAG CGACCAGGCA 
GCGACGATCA ATGCCATGGA GTCAAAGATG ACCAGTCTGG AGCAGAGGAT CGCTGAGCTG 
TCCGAAGCCA ACAAACTGGC AGCAAACAGC AGCATTTACA CACAGAAGAA CATGAAAGCC 
CAGGAGGAGA TGATTTCTGA ACTCAGACAG CAGAAGTTTT ATTTGGAGTC TCAGGCTGGA 
AAACTGGAGG CGCAGAATGC AAAGCTGGAG GAGCATCTGG AGAAACTCAC CCAACAAGAG 
CAGACAAACA AGAGCCGAGT GCTGGAGCTG GAAGCCAGGC TGCGAGAGAT CGGCCTAGAG 
CATGAGGAAC AGAAGCTGGA GATCAAGCGT CAGGTGTCGG AGCTCACGTT GTCTCTGCAG 
GAGAGAGAAG CTCAGATCAG CAGCCTGCAA GCGGCACGCC ACGCTCTCGA CAGCCAACTA 
CAGCAGGCCA AAACTGAGCT GGAGGAGACC ACCGCCGAGG CCGAAGAGGA GATCACCGTC 
CTGAGGGCCC ATCGGGATGA AATCCAACGC AAGTTTGATG CCCTGCGAGA CAGCTGTGCG 
GTGATCACGG ATCTGGAGGA GCAGTTGACG CAGCTCACGC AAGAAAACGC GGAGCTGAAC 
CGGCAGAACT TCTACCTGTC CAAACAGCTG GACGAGGCCA CAGACGAGAA CGAGGAGCGC 
CTGCAGCTCA AGCAGGACGT GGAGCGCCTC AGGAGAGAGG TGGCAGACCG AGAGATGCAC 
CTCAACAACC AGAAACAGGT GACACGGCAA CACACACACA CACACACACA CACACACACA 
CACAGGACAG AGGTGGCAGA CCGAGAGATG CACCTCAACA ACCAGAAACA GAACATCGAG 
ACGCTGAAGA CGACATGTAC AATGCTGGAG GAGCAGGTTA TGGAGCTGGA GACTCTGAAT 
GATGAGCTGA TGGAGAAGGA GAGGCAGTGG GAGAACTGGA GATCTGCTCT GGACGAGGAG 
AAAAACCAGG CCGAGCGCAG AGCCAGAGAC CTGCAAAGAC AGCTGGACAG CGAGAAGCAG 
AACCGACTGC GTGCAGAACA GAGAAACTCT GAGTCTCGGC AGGCCGTGGA GCTGGCGGTG 
AAAGAGCACA AAGCTGAAAT CCTGGTTCTG CAGCAGGCGC TCAAAGACCA GAAACTCAAG 
GCTGAGGGCC TTTCTGACAC TCTGGGTGAT CTGGAGAAGA AGCACGGCAT GCTGGAGATG 
AACGCTCGCA GCCTGCAGCA GAAGCTGGAG ACGGAGAGAG AGATGAAGCA GAGACTCATG 
GACGAGCAAG CTAAACTGCA GCAGCAGCTG GACATGCAGA AGAGCCACAT CTTCAGACTG 
ACTCAAGGAC TGCAGGATGC GCTGGACCAG ACCGACCTGC TGAAGACCGA GCGCACAGAC 
CTGGAGTACC AGCTGGAAAA CATACAGGCG GCGTATTCTC ACGAGAAGGT GAAGATGGAG 
GGGACGATCA CCCAGCAGAC CAAGCTCATA GACTTCCTGC AGGCCAAGGT AGAGCACCCC 
TCTAAGAAGA AAAAGGGGCT GTTTGGTGGC CGGCGGCGGG AGGATCTGCT AGCGGCGCTG 
GCGGCTCAGG GACAGACACA GGTGCCTGTG GTGCCCGCCG CCACTCCTGT GCCCCTGCAG 
TACAGCGACC TGAAGGCGGC GCTGGATAAA GAGCGAGCCC GATGCTCCGA GCTGGAGGAC 
GCCCTGCAGA AAACACGCCT GGAGCTCAGA GAAGTTCAGT TTAATAAAGC TCTGGAGCAC 
AGCTCTTCTC CAGCCACTCC AGGTTTGGCC CGTCAGCAGC TCATGATGTC CACCAAGTCC 
AACAAGTCTC CAGAGCACCA GTCCAGCGGC GGCCTGATGT CCTCAAACTC CAGCCGCGGA 
CGCAAAGACA ACGCCAATCC TGAAGAGTAC ACCAGACGTG TGAAGGACCG GATTCATCAC 
AACACTCCTC ACCGCTTCAC GCAGGGGCTC AATATGAGAG CGGCTCGCTG TACTGTGTGT 
CTGGACACAG TCCATTTTGG ACGTCAGGCT GCAACATGCA TTGAATGTCA CGCGTTGTGT 
CACCCAAAAT GCTCCCCGTA CCTCCCTGCC ACCTGCGGCG TTCCCACTGA ATACGCGCTG 
CACCTCTCAG AGGCTCTGTG TCGGGAGAAG GGAAGCACTT CCGGCCTGAA AGATATGGGT 
GGACATATGA GGCTTGAAGG ATGGCTGAAG CAGCCCAGGA ACGGGAAGCG CGGGCAGGGC 
TGGGAGAGGA AGTATGTGGT GCTGGATGGA TATAAAGTGA TTACATTCGA GACAGAACCA 
AGAGACGAGT CAGTGAAGCC GCTGGATGAG TTTGAGCTGT GTCTCTCTGA TGGAGAAGTG 
ACCGTTCATT CTGCTGTGGG TTCCTCTGAG CTCCCCAACA CCGCTAAAAC AGATGTGCCG 
TACGTGCTGA AGCTGGAGTC GTCTCCTCAG TCCACATGCT GGCCCGGTCA GACTCTGTAC 
CTCATGACCT CGGGATACTC AGAGAAGCAG CGCTGGGTGG CAGTGCTGGA AGCCATCGTG 
GCCAGCAGTC GCGGAGCCAG AGAGAAAGCT GAGGCAGATG CTAAATTATT GGGTAACTCT 
TTACTTAAGC TAGAAGGTGA CGACCGTCTG GATATCAACT GCACCCTGCC GCTGACCGAC 
CAGATTGTGT TGGTGGGCTC TGAGGAGGGT CTCTACGCAC TGAATGTGAT TAAGAACTCC 
CTCACTCATA TCCCGGGTCT GGGATCCGTG TTTCAGATCC ACATTATCAA AGATCATGAC 
AAGCTGCTGA TGATCGTGGG AGACGAGCGA GCGCTGTGTC TGGTGGAGAT TAAGAAGGTG 
AAGCAGTCTT TATCCCAGTC TCACCTCCCC ACACTGCCCG AGATTGTGCC GTATATCTTT 
GAGACCGTCA AGGGCTGTCA TCTGTTTGCC TCGGGCAGGA TAGAAAATGG CCCATGCATT 
TGTGCTGCGA TGCCCAATAA AGTCACGATT CTACGATACA ATGACAATCT TAACAAGTTC 
TGCATACGGA AGGAGATTGA AACTCTGGAG CCGTGCAGCT GCATCCACTT CACCAGCTAC 
AGCATCATCA TTGGTACCAA TAAATTCTAC GAGATCGAGA TGAAGCAGTG CGTTCTGGAC 
GAGTTCCTGG ACAAGAATGA CGTGTCGCTG GCGTCGGCTG TCTTCACCGG ATCCTCCCAC 
AGTTTCCCCA TCTCTATCGT CCAGGTGTCC AGCACTCCGC AGAAGGAGGA ATATCTGCTG 
TGTTTCCACG AGATCGGAGT TTTTGTGGAT GCTTACGGTC GAAGAAGTCG CCCTGATGAT 
CTGAAGTGGA GTCGACTTCC CCTGACGTTC GCCTTCAGAG AGCCGTATCT CTTTGTGACC 
TACTTCAACT CTCTGGATGT TATTGAGATT CAGGGACACG CAGCTCTTGG GCCTCCCAGT 
CTGGTTCACC TGGACATTCC TAACCCACGT TATCTGGGTC CAGCCATATC GTCTGGTGCC 
ATCTATCTGG CCTCGTCCTA CCAGAACAAA CTGCGGGTGA TCTGCTGTAA GGGCAGTCTG 
GTGCGGGAGT CTGGAGAGCT GCAGAGGACC GGCTCCAGCA GGAGCCCCAA CAAGCGCGGT 
CCACCAACAT ACAACGAGCA CATCTCCAAA CGTCTGGCCT CGAGTCCTGG AGCCCAGGAG 
GGGCCCCTGC ACCGCGAGCC CAGCACTCCT CACCGCTACC GCGAGGGCCG CACCGAGTTC 
CGGAGAGACA AATCTCCAGC CCGGCCCCTG GAGCGAGAGA AATCCCCGGG CCGTCCGGTG 
CTAGACAGCC GCAGAGAGAG GTCTCCCGGT CGCTTCGAGA GCAGCGGCAG CGAGAGAGAC 
CGCGAGCGGA CACGACTGCA CACTAGCTCT GTACGCACCC AGCTGACCCC CGTCAACAAG 
GTTTGGGACC AGTCTTCGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212893.1
CDS	433..5376
Translation	MEQEISKVQRKMSDLESVLQQKDVELKASETQRNILEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSNKLQEIKEQEYHAQLEEMRVSIRQLEENLSAARRRSDLYESELKESRQTSEELKRKAVEYQQKIQKAKEQGKVEMEELLSKLQKTNGEQQVKIEELQEKLAKAVKASTEATELLQNVRQAKERMERDLERLQTKEDSSDSLRRRLRETEDGKKSLENQVKRLEMVERRESKLKDDIQTKSQQIQQMADKIMELEENLRDSQAMAQRMETQLEQKEKLFEDKIKVLETQMKADLAEKDLLESKQSTFEEESRERGKVISDQAATINAMESKMTSLEQRIAELSEANKLAANSSIYTQKNMKAQEEMISELRQQKFYLESQAGKLEAQNAKLEEHLEKLTQQEQTNKSRVLELEARLREIGLEHEEQKLEIKRQVSELTLSLQEREAQISSLQAARHALDSQLQQAKTELEETTAEAEEEITVLRAHRDEIQRKFDALRDSCAVITDLEEQLTQLTQENAELNRQNFYLSKQLDEATDENEERLQLKQDVERLRREVADREMHLNNQKQVTRQHTHTHTHTHTHRTEVADREMHLNNQKQNIETLKTTCTMLEEQVMELETLNDELMEKERQWENWRSALDEEKNQAERRARDLQRQLDSEKQNRLRAEQRNSESRQAVELAVKEHKAEILVLQQALKDQKLKAEGLSDTLGDLEKKHGMLEMNARSLQQKLETEREMKQRLMDEQAKLQQQLDMQKSHIFRLTQGLQDALDQTDLLKTERTDLEYQLENIQAAYSHEKVKMEGTITQQTKLIDFLQAKVEHPSKKKKGLFGGRRREDLLAALAAQGQTQVPVVPAATPVPLQYSDLKAALDKERARCSELEDALQKTRLELREVQFNKALEHSSSPATPGLARQQLMMSTKSNKSPEHQSSGGLMSSNSSRGRKDNANPEEYTRRVKDRIHHNTPHRFTQGLNMRAARCTVCLDTVHFGRQAATCIECHALCHPKCSPYLPATCGVPTEYALHLSEALCREKGSTSGLKDMGGHMRLEGWLKQPRNGKRGQGWERKYVVLDGYKVITFETEPRDESVKPLDEFELCLSDGEVTVHSAVGSSELPNTAKTDVPYVLKLESSPQSTCWPGQTLYLMTSGYSEKQRWVAVLEAIVASSRGAREKAEADAKLLGNSLLKLEGDDRLDINCTLPLTDQIVLVGSEEGLYALNVIKNSLTHIPGLGSVFQIHIIKDHDKLLMIVGDERALCLVEIKKVKQSLSQSHLPTLPEIVPYIFETVKGCHLFASGRIENGPCICAAMPNKVTILRYNDNLNKFCIRKEIETLEPCSCIHFTSYSIIIGTNKFYEIEMKQCVLDEFLDKNDVSLASAVFTGSSHSFPISIVQVSSTPQKEEYLLCFHEIGVFVDAYGRRSRPDDLKWSRLPLTFAFREPYLFVTYFNSLDVIEIQGHAALGPPSLVHLDIPNPRYLGPAISSGAIYLASSYQNKLRVICCKGSLVRESGELQRTGSSRSPNKRGPPTYNEHISKRLASSPGAQEGPLHREPSTPHRYREGRTEFRRDKSPARPLEREKSPGRPVLDSRRERSPGRFESSGSERDRERTRLHTSSVRTQLTPVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_017357404.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357404.2

ORF Insert Sequence:

ATGGAGCAGG AGATTTCCAA AGTGCAGCGT AAGATGTCAG ACTTGGAGTC AGTTTTGCAG 
CAGAAAGACG TGGAGCTCAA GGCTTCAGAA ACCCAGAGAA ACATCTTAGA GCAAGACCTG 
GCAACCTATA TCACCGAATG CAGCAGTTTG AAGCGCAGTT TGGAGCAGGC CCGCATGGAG 
GTTTCGCAGG AAGATGATAA AGCTCTTCAG CTGCTGCACG ACATTCGAGA GCAGAGCAAC 
AAACTGCAGG AAATCAAAGA ACAGGAGTAC CACGCTCAGC TGGAGGAGAT GCGAGTGTCT 
ATTCGTCAGC TGGAAGAGAA TCTGTCGGCC GCTCGTCGCC GCAGTGACCT GTACGAGTCT 
GAGCTCAAAG AGTCCCGTCA GACCAGTGAG GAGCTCAAGA GGAAGGCAGT GGAGTATCAG 
CAGAAGATCC AGAAGGCTAA AGAACAAGGG AAAGTTGAGA TGGAGGAGCT TCTGAGCAAA 
CTGCAGAAGA CCAATGGAGA GCAACAAGTA AAGATTGAAG AGCTCCAGGA GAAGCTTGCT 
AAGGCAGTGA AGGCGAGTAC TGAAGCCACT GAACTCCTCC AGAATGTCCG TCAGGCTAAA 
GAGCGAATGG AGCGAGACCT GGAGCGTCTG CAGACCAAAG AGGACTCGTC CGACAGCCTG 
CGCAGACGCT TACGAGAGAC TGAGGATGGC AAAAAATCCC TGGAGAACCA GGTGAAGAGG 
CTGGAGATGG TGGAGCGGAG AGAATCCAAA CTGAAAGACG ACATTCAGAC CAAATCGCAG 
CAGATCCAGC AGATGGCCGA CAAAATCATG GAGCTTGAAG AAAATTTGCG GGACTCGCAG 
GCGATGGCGC AGCGCATGGA AACACAGCTG GAGCAGAAGG AAAAGCTTTT TGAAGACAAG 
ATCAAGGTTT TAGAAACTCA AATGAAGGCT GATTTGGCTG AGAAGGATTT GCTGGAGTCC 
AAACAGAGCA CTTTTGAAGA GGAGTCTCGC GAACGGGGAA AAGTGATCAG CGACCAGGCA 
GCGACGATCA ATGCCATGGA GTCAAAGATG ACCAGTCTGG AGCAGAGGAT CGCTGAGCTG 
TCCGAAGCCA ACAAACTGGC AGCAAACAGC AGCATTTACA CACAGAAGAA CATGAAAGCC 
CAGGAGGAGA TGATTTCTGA ACTCAGACAG CAGAAGTTTT ATTTGGAGTC TCAGGCTGGA 
AAACTGGAGG CGCAGAATGC AAAGCTGGAG GAGCATCTGG AGAAACTCAC CCAACAAGAG 
CAGACAAACA AGAGCCGAGT GCTGGAGCTG GAAGCCAGGC TGCGAGAGAT CGGCCTAGAG 
CATGAGGAAC AGAAGCTGGA GATCAAGCGT CAGGTGTCGG AGCTCACGTT GTCTCTGCAG 
GAGAGAGAAG CTCAGATCAG CAGCCTGCAA GCGGCACGCC ACGCTCTCGA CAGCCAACTA 
CAGCAGGCCA AAACTGAGCT GGAGGAGACC ACCGCCGAGG CCGAAGAGGA GATCACCGTC 
CTGAGGGCCC ATCGGGATGA AATCCAACGC AAGTTTGATG CCCTGCGAGA CAGCTGTGCG 
GTGATCACGG ATCTGGAGGA GCAGTTGACG CAGCTCACGC AAGAAAACGC GGAGCTGAAC 
CGGCAGAACT TCTACCTGTC CAAACAGCTG GACGAGGCCA CAGACGAGAA CGAGGAGCGC 
CTGCAGCTCA AGCAGGACGT GGAGCGCCTC AGGAGAGAGG TGGCAGACCG AGAGATGCAC 
CTCAACAACC AGAAACAGGT GACACGGCAA CACACACACA CACACACACA CACACACACA 
CACAGGACAG AGGTGGCAGA CCGAGAGATG CACCTCAACA ACCAGAAACA GAACATCGAG 
ACGCTGAAGA CGACATGTAC AATGCTGGAG GAGCAGGTTA TGGAGCTGGA GACTCTGAAT 
GATGAGCTGA TGGAGAAGGA GAGGCAGTGG GAGAACTGGA GATCTGCTCT GGACGAGGAG 
AAAAACCAGG CCGAGCGCAG AGCCAGAGAC CTGCAAAGAC AGCTGGACAG CGAGAAGCAG 
AACCGACTGC GTGCAGAACA GAGAAACTCT GAGTCTCGGC AGGCCGTGGA GCTGGCGGTG 
AAAGAGCACA AAGCTGAAAT CCTGGTTCTG CAGCAGGCGC TCAAAGACCA GAAACTCAAG 
GCTGAGGGCC TTTCTGACAC TCTGGGTGAT CTGGAGAAGA AGCACGGCAT GCTGGAGATG 
AACGCTCGCA GCCTGCAGCA GAAGCTGGAG ACGGAGAGAG AGATGAAGCA GAGACTCATG 
GACGAGCAAG CTAAACTGCA GCAGCAGCTG GACATGCAGA AGAGCCACAT CTTCAGACTG 
ACTCAAGGAC TGCAGGATGC GCTGGACCAG ACCGACCTGC TGAAGACCGA GCGCACAGAC 
CTGGAGTACC AGCTGGAAAA CATACAGGCG GCGTATTCTC ACGAGAAGGT GAAGATGGAG 
GGGACGATCA CCCAGCAGAC CAAGCTCATA GACTTCCTGC AGGCCAAGGT AGAGCACCCC 
TCTAAGAAGA AAAAGGGGCT GTTTGGTGGC CGGCGGCGGG AGGATCTGCT AGCGGCGCTG 
GCGGCTCAGG GACAGACACA GGTGCCTGTG GTGCCCGCCG CCACTCCTGT GCCCCTGCAG 
TACAGCGACC TGAAGGCGGC GCTGGATAAA GAGCGAGCCC GATGCTCCGA GCTGGAGGAC 
GCCCTGCAGA AAACACGCCT GGAGCTCAGA GAAGTTCAGT TTAATAAAGC TCTGGAGCAC 
AGCTCTTCTC CAGCCACTCC AGGTTTGGCC CGTCAGCAGC TCATGATGTC CACCAAGTCC 
AACAAGTCTC CAGAGCACCA GTCCAGCGGC GGCCTGATGT CCTCAAACTC CAGCCGCGGA 
CGCAAAGACA ACGCCAATCC TGAAGAGTAC ACCAGACGTG TGAAGGACCG GATTCATCAC 
AACACTCCTC ACCGCTTCAC GCAGGGGCTC AATATGAGAG CGGCTCGCTG TACTGTGTGT 
CTGGACACAG TCCATTTTGG ACGTCAGGCT GCAACATGCA TTGAATGTCA CGCGTTGTGT 
CACCCAAAAT GCTCCCCGTA CCTCCCTGCC ACCTGCGGCG TTCCCACTGA ATACGCGCTG 
CACCTCTCAG AGGCTCTGTG TCGGGAGAAG GGAAGCACTT CCGGCCTGAA AGATATGGGT 
GGACATATGA GGCTTGAAGG ATGGCTGAAG CAGCCCAGGA ACGGGAAGCG CGGGCAGGGC 
TGGGAGAGGA AGTATGTGGT GCTGGATGGA TATAAAGTGA TTACATTCGA GACAGAACCA 
AGAGACGAGT CAGTGAAGCC GCTGGATGAG TTTGAGCTGT GTCTCTCTGA TGGAGAAGTG 
ACCGTTCATT CTGCTGTGGG TTCCTCTGAG CTCCCCAACA CCGCTAAAAC AGATGTGCCG 
TACGTGCTGA AGCTGGAGTC GTCTCCTCAG TCCACATGCT GGCCCGGTCA GACTCTGTAC 
CTCATGACCT CGGGATACTC AGAGAAGCAG CGCTGGGTGG CAGTGCTGGA AGCCATCGTG 
GCCAGCAGTC GCGGAGCCAG AGAGAAAGCT GAGGCAGATG CTAAATTATT GGGTAACTCT 
TTACTTAAGC TAGAAGGTGA CGACCGTCTG GATATCAACT GCACCCTGCC GCTGACCGAC 
CAGATTGTGT TGGTGGGCTC TGAGGAGGGT CTCTACGCAC TGAATGTGAT TAAGAACTCC 
CTCACTCATA TCCCGGGTCT GGGATCCGTG TTTCAGATCC ACATTATCAA AGATCATGAC 
AAGCTGCTGA TGATCGTGGG AGACGAGCGA GCGCTGTGTC TGGTGGAGAT TAAGAAGGTG 
AAGCAGTCTT TATCCCAGTC TCACCTCCCC ACACTGCCCG AGATTGTGCC GTATATCTTT 
GAGACCGTCA AGGGCTGTCA TCTGTTTGCC TCGGGCAGGA TAGAAAATGG CCCATGCATT 
TGTGCTGCGA TGCCCAATAA AGTCACGATT CTACGATACA ATGACAATCT TAACAAGTTC 
TGCATACGGA AGGAGATTGA AACTCTGGAG CCGTGCAGCT GCATCCACTT CACCAGCTAC 
AGCATCATCA TTGGTACCAA TAAATTCTAC GAGATCGAGA TGAAGCAGTG CGTTCTGGAC 
GAGTTCCTGG ACAAGAATGA CGTGTCGCTG GCGTCGGCTG TCTTCACCGG ATCCTCCCAC 
AGTTTCCCCA TCTCTATCGT CCAGGTGTCC AGCACTCCGC AGAAGGAGGA ATATCTGCTG 
TGTTTCCACG AGATCGGAGT TTTTGTGGAT GCTTACGGTC GAAGAAGTCG CCCTGATGAT 
CTGAAGTGGA GTCGACTTCC CCTGACGTTC GCCTTCAGAG AGCCGTATCT CTTTGTGACC 
TACTTCAACT CTCTGGATGT TATTGAGATT CAGGGACACG CAGCTCTTGG GCCTCCCAGT 
CTGGTTCACC TGGACATTCC TAACCCACGT TATCTGGGTC CAGCCATATC GTCTGGTGCC 
ATCTATCTGG CCTCGTCCTA CCAGAACAAA CTGCGGGTGA TCTGCTGTAA GGGCAGTCTG 
GTGCGGGAGT CTGGAGAGCT GCAGAGGACC GGCTCCAGCA GGAGCCCCAA CAAGCGCGGT 
CCACCAACAT ACAACGAGCA CATCTCCAAA CGTCTGGCCT CGAGTCCTGG AGCCCAGGAG 
GGGCCCCTGC ACCGCGAGCC CAGCACTCCT CACCGCTACC GCGAGGGCCG CACCGAGTTC 
CGGAGAGACA AATCTCCAGC CCGGCCCCTG GAGCGAGAGA AATCCCCGGG CCGTCCGGTG 
CTAGACAGCC GCAGAGAGAG GTCTCCCGGT CGCTTCGAGA GCAGCGGCAG CGAGAGAGAC 
CGCGAGCGGA CACGACTGCA CACTAGCTCT GTACGCACCC AGCTGACCCC CGTCAACAAG 
GTTTGGGACC AGTCTTCGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100333880 ( XM_017357404.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212893.1 Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X7, mRNA.

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CloneID	ODa60010
Clone ID Related Accession (Same CDS sequence)	XM_017357404.2 , XM_017357404.1
Accession Version	XM_017357404.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	citron Rho-interacting kinase-like isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG AGATTTCCAA AGTGCAGCGT AAGATGTCAG ACTTGGAGTC AGTTTTGCAG 
CAGAAAGACG TGGAGCTCAA GGCTTCAGAA ACCCAGAGAA ACATCTTAGA GCAAGACCTG 
GCAACCTATA TCACCGAATG CAGCAGTTTG AAGCGCAGTT TGGAGCAGGC CCGCATGGAG 
GTTTCGCAGG AAGATGATAA AGCTCTTCAG CTGCTGCACG ACATTCGAGA GCAGAGCAAC 
AAACTGCAGG AAATCAAAGA ACAGGAGTAC CACGCTCAGC TGGAGGAGAT GCGAGTGTCT 
ATTCGTCAGC TGGAAGAGAA TCTGTCGGCC GCTCGTCGCC GCAGTGACCT GTACGAGTCT 
GAGCTCAAAG AGTCCCGTCA GACCAGTGAG GAGCTCAAGA GGAAGGCAGT GGAGTATCAG 
CAGAAGATCC AGAAGGCTAA AGAACAAGGG AAAGTTGAGA TGGAGGAGCT TCTGAGCAAA 
CTGCAGAAGA CCAATGGAGA GCAACAAGTA AAGATTGAAG AGCTCCAGGA GAAGCTTGCT 
AAGGCAGTGA AGGCGAGTAC TGAAGCCACT GAACTCCTCC AGAATGTCCG TCAGGCTAAA 
GAGCGAATGG AGCGAGACCT GGAGCGTCTG CAGACCAAAG AGGACTCGTC CGACAGCCTG 
CGCAGACGCT TACGAGAGAC TGAGGATGGC AAAAAATCCC TGGAGAACCA GGTGAAGAGG 
CTGGAGATGG TGGAGCGGAG AGAATCCAAA CTGAAAGACG ACATTCAGAC CAAATCGCAG 
CAGATCCAGC AGATGGCCGA CAAAATCATG GAGCTTGAAG AAAATTTGCG GGACTCGCAG 
GCGATGGCGC AGCGCATGGA AACACAGCTG GAGCAGAAGG AAAAGCTTTT TGAAGACAAG 
ATCAAGGTTT TAGAAACTCA AATGAAGGCT GATTTGGCTG AGAAGGATTT GCTGGAGTCC 
AAACAGAGCA CTTTTGAAGA GGAGTCTCGC GAACGGGGAA AAGTGATCAG CGACCAGGCA 
GCGACGATCA ATGCCATGGA GTCAAAGATG ACCAGTCTGG AGCAGAGGAT CGCTGAGCTG 
TCCGAAGCCA ACAAACTGGC AGCAAACAGC AGCATTTACA CACAGAAGAA CATGAAAGCC 
CAGGAGGAGA TGATTTCTGA ACTCAGACAG CAGAAGTTTT ATTTGGAGTC TCAGGCTGGA 
AAACTGGAGG CGCAGAATGC AAAGCTGGAG GAGCATCTGG AGAAACTCAC CCAACAAGAG 
CAGACAAACA AGAGCCGAGT GCTGGAGCTG GAAGCCAGGC TGCGAGAGAT CGGCCTAGAG 
CATGAGGAAC AGAAGCTGGA GATCAAGCGT CAGGTGTCGG AGCTCACGTT GTCTCTGCAG 
GAGAGAGAAG CTCAGATCAG CAGCCTGCAA GCGGCACGCC ACGCTCTCGA CAGCCAACTA 
CAGCAGGCCA AAACTGAGCT GGAGGAGACC ACCGCCGAGG CCGAAGAGGA GATCACCGTC 
CTGAGGGCCC ATCGGGATGA AATCCAACGC AAGTTTGATG CCCTGCGAGA CAGCTGTGCG 
GTGATCACGG ATCTGGAGGA GCAGTTGACG CAGCTCACGC AAGAAAACGC GGAGCTGAAC 
CGGCAGAACT TCTACCTGTC CAAACAGCTG GACGAGGCCA CAGACGAGAA CGAGGAGCGC 
CTGCAGCTCA AGCAGGACGT GGAGCGCCTC AGGAGAGAGG TGGCAGACCG AGAGATGCAC 
CTCAACAACC AGAAACAGGT GACACGGCAA CACACACACA CACACACACA CACACACACA 
CACAGGACAG AGGTGGCAGA CCGAGAGATG CACCTCAACA ACCAGAAACA GAACATCGAG 
ACGCTGAAGA CGACATGTAC AATGCTGGAG GAGCAGGTTA TGGAGCTGGA GACTCTGAAT 
GATGAGCTGA TGGAGAAGGA GAGGCAGTGG GAGAACTGGA GATCTGCTCT GGACGAGGAG 
AAAAACCAGG CCGAGCGCAG AGCCAGAGAC CTGCAAAGAC AGCTGGACAG CGAGAAGCAG 
AACCGACTGC GTGCAGAACA GAGAAACTCT GAGTCTCGGC AGGCCGTGGA GCTGGCGGTG 
AAAGAGCACA AAGCTGAAAT CCTGGTTCTG CAGCAGGCGC TCAAAGACCA GAAACTCAAG 
GCTGAGGGCC TTTCTGACAC TCTGGGTGAT CTGGAGAAGA AGCACGGCAT GCTGGAGATG 
AACGCTCGCA GCCTGCAGCA GAAGCTGGAG ACGGAGAGAG AGATGAAGCA GAGACTCATG 
GACGAGCAAG CTAAACTGCA GCAGCAGCTG GACATGCAGA AGAGCCACAT CTTCAGACTG 
ACTCAAGGAC TGCAGGATGC GCTGGACCAG ACCGACCTGC TGAAGACCGA GCGCACAGAC 
CTGGAGTACC AGCTGGAAAA CATACAGGCG GCGTATTCTC ACGAGAAGGT GAAGATGGAG 
GGGACGATCA CCCAGCAGAC CAAGCTCATA GACTTCCTGC AGGCCAAGGT AGAGCACCCC 
TCTAAGAAGA AAAAGGGGCT GTTTGGTGGC CGGCGGCGGG AGGATCTGCT AGCGGCGCTG 
GCGGCTCAGG GACAGACACA GGTGCCTGTG GTGCCCGCCG CCACTCCTGT GCCCCTGCAG 
TACAGCGACC TGAAGGCGGC GCTGGATAAA GAGCGAGCCC GATGCTCCGA GCTGGAGGAC 
GCCCTGCAGA AAACACGCCT GGAGCTCAGA GAAGTTCAGT TTAATAAAGC TCTGGAGCAC 
AGCTCTTCTC CAGCCACTCC AGGTTTGGCC CGTCAGCAGC TCATGATGTC CACCAAGTCC 
AACAAGTCTC CAGAGCACCA GTCCAGCGGC GGCCTGATGT CCTCAAACTC CAGCCGCGGA 
CGCAAAGACA ACGCCAATCC TGAAGAGTAC ACCAGACGTG TGAAGGACCG GATTCATCAC 
AACACTCCTC ACCGCTTCAC GCAGGGGCTC AATATGAGAG CGGCTCGCTG TACTGTGTGT 
CTGGACACAG TCCATTTTGG ACGTCAGGCT GCAACATGCA TTGAATGTCA CGCGTTGTGT 
CACCCAAAAT GCTCCCCGTA CCTCCCTGCC ACCTGCGGCG TTCCCACTGA ATACGCGCTG 
CACCTCTCAG AGGCTCTGTG TCGGGAGAAG GGAAGCACTT CCGGCCTGAA AGATATGGGT 
GGACATATGA GGCTTGAAGG ATGGCTGAAG CAGCCCAGGA ACGGGAAGCG CGGGCAGGGC 
TGGGAGAGGA AGTATGTGGT GCTGGATGGA TATAAAGTGA TTACATTCGA GACAGAACCA 
AGAGACGAGT CAGTGAAGCC GCTGGATGAG TTTGAGCTGT GTCTCTCTGA TGGAGAAGTG 
ACCGTTCATT CTGCTGTGGG TTCCTCTGAG CTCCCCAACA CCGCTAAAAC AGATGTGCCG 
TACGTGCTGA AGCTGGAGTC GTCTCCTCAG TCCACATGCT GGCCCGGTCA GACTCTGTAC 
CTCATGACCT CGGGATACTC AGAGAAGCAG CGCTGGGTGG CAGTGCTGGA AGCCATCGTG 
GCCAGCAGTC GCGGAGCCAG AGAGAAAGCT GAGGCAGATG CTAAATTATT GGGTAACTCT 
TTACTTAAGC TAGAAGGTGA CGACCGTCTG GATATCAACT GCACCCTGCC GCTGACCGAC 
CAGATTGTGT TGGTGGGCTC TGAGGAGGGT CTCTACGCAC TGAATGTGAT TAAGAACTCC 
CTCACTCATA TCCCGGGTCT GGGATCCGTG TTTCAGATCC ACATTATCAA AGATCATGAC 
AAGCTGCTGA TGATCGTGGG AGACGAGCGA GCGCTGTGTC TGGTGGAGAT TAAGAAGGTG 
AAGCAGTCTT TATCCCAGTC TCACCTCCCC ACACTGCCCG AGATTGTGCC GTATATCTTT 
GAGACCGTCA AGGGCTGTCA TCTGTTTGCC TCGGGCAGGA TAGAAAATGG CCCATGCATT 
TGTGCTGCGA TGCCCAATAA AGTCACGATT CTACGATACA ATGACAATCT TAACAAGTTC 
TGCATACGGA AGGAGATTGA AACTCTGGAG CCGTGCAGCT GCATCCACTT CACCAGCTAC 
AGCATCATCA TTGGTACCAA TAAATTCTAC GAGATCGAGA TGAAGCAGTG CGTTCTGGAC 
GAGTTCCTGG ACAAGAATGA CGTGTCGCTG GCGTCGGCTG TCTTCACCGG ATCCTCCCAC 
AGTTTCCCCA TCTCTATCGT CCAGGTGTCC AGCACTCCGC AGAAGGAGGA ATATCTGCTG 
TGTTTCCACG AGATCGGAGT TTTTGTGGAT GCTTACGGTC GAAGAAGTCG CCCTGATGAT 
CTGAAGTGGA GTCGACTTCC CCTGACGTTC GCCTTCAGAG AGCCGTATCT CTTTGTGACC 
TACTTCAACT CTCTGGATGT TATTGAGATT CAGGGACACG CAGCTCTTGG GCCTCCCAGT 
CTGGTTCACC TGGACATTCC TAACCCACGT TATCTGGGTC CAGCCATATC GTCTGGTGCC 
ATCTATCTGG CCTCGTCCTA CCAGAACAAA CTGCGGGTGA TCTGCTGTAA GGGCAGTCTG 
GTGCGGGAGT CTGGAGAGCT GCAGAGGACC GGCTCCAGCA GGAGCCCCAA CAAGCGCGGT 
CCACCAACAT ACAACGAGCA CATCTCCAAA CGTCTGGCCT CGAGTCCTGG AGCCCAGGAG 
GGGCCCCTGC ACCGCGAGCC CAGCACTCCT CACCGCTACC GCGAGGGCCG CACCGAGTTC 
CGGAGAGACA AATCTCCAGC CCGGCCCCTG GAGCGAGAGA AATCCCCGGG CCGTCCGGTG 
CTAGACAGCC GCAGAGAGAG GTCTCCCGGT CGCTTCGAGA GCAGCGGCAG CGAGAGAGAC 
CGCGAGCGGA CACGACTGCA CACTAGCTCT GTACGCACCC AGCTGACCCC CGTCAACAAG 
GTTTGGGACC AGTCTTCGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212893.1
CDS	434..5377
Translation	MEQEISKVQRKMSDLESVLQQKDVELKASETQRNILEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSNKLQEIKEQEYHAQLEEMRVSIRQLEENLSAARRRSDLYESELKESRQTSEELKRKAVEYQQKIQKAKEQGKVEMEELLSKLQKTNGEQQVKIEELQEKLAKAVKASTEATELLQNVRQAKERMERDLERLQTKEDSSDSLRRRLRETEDGKKSLENQVKRLEMVERRESKLKDDIQTKSQQIQQMADKIMELEENLRDSQAMAQRMETQLEQKEKLFEDKIKVLETQMKADLAEKDLLESKQSTFEEESRERGKVISDQAATINAMESKMTSLEQRIAELSEANKLAANSSIYTQKNMKAQEEMISELRQQKFYLESQAGKLEAQNAKLEEHLEKLTQQEQTNKSRVLELEARLREIGLEHEEQKLEIKRQVSELTLSLQEREAQISSLQAARHALDSQLQQAKTELEETTAEAEEEITVLRAHRDEIQRKFDALRDSCAVITDLEEQLTQLTQENAELNRQNFYLSKQLDEATDENEERLQLKQDVERLRREVADREMHLNNQKQVTRQHTHTHTHTHTHRTEVADREMHLNNQKQNIETLKTTCTMLEEQVMELETLNDELMEKERQWENWRSALDEEKNQAERRARDLQRQLDSEKQNRLRAEQRNSESRQAVELAVKEHKAEILVLQQALKDQKLKAEGLSDTLGDLEKKHGMLEMNARSLQQKLETEREMKQRLMDEQAKLQQQLDMQKSHIFRLTQGLQDALDQTDLLKTERTDLEYQLENIQAAYSHEKVKMEGTITQQTKLIDFLQAKVEHPSKKKKGLFGGRRREDLLAALAAQGQTQVPVVPAATPVPLQYSDLKAALDKERARCSELEDALQKTRLELREVQFNKALEHSSSPATPGLARQQLMMSTKSNKSPEHQSSGGLMSSNSSRGRKDNANPEEYTRRVKDRIHHNTPHRFTQGLNMRAARCTVCLDTVHFGRQAATCIECHALCHPKCSPYLPATCGVPTEYALHLSEALCREKGSTSGLKDMGGHMRLEGWLKQPRNGKRGQGWERKYVVLDGYKVITFETEPRDESVKPLDEFELCLSDGEVTVHSAVGSSELPNTAKTDVPYVLKLESSPQSTCWPGQTLYLMTSGYSEKQRWVAVLEAIVASSRGAREKAEADAKLLGNSLLKLEGDDRLDINCTLPLTDQIVLVGSEEGLYALNVIKNSLTHIPGLGSVFQIHIIKDHDKLLMIVGDERALCLVEIKKVKQSLSQSHLPTLPEIVPYIFETVKGCHLFASGRIENGPCICAAMPNKVTILRYNDNLNKFCIRKEIETLEPCSCIHFTSYSIIIGTNKFYEIEMKQCVLDEFLDKNDVSLASAVFTGSSHSFPISIVQVSSTPQKEEYLLCFHEIGVFVDAYGRRSRPDDLKWSRLPLTFAFREPYLFVTYFNSLDVIEIQGHAALGPPSLVHLDIPNPRYLGPAISSGAIYLASSYQNKLRVICCKGSLVRESGELQRTGSSRSPNKRGPPTYNEHISKRLASSPGAQEGPLHREPSTPHRYREGRTEFRRDKSPARPLEREKSPGRPVLDSRRERSPGRFESSGSERDRERTRLHTSSVRTQLTPVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_017357404.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357404.1

ORF Insert Sequence:

ATGGAGCAGG AGATTTCCAA AGTGCAGCGT AAGATGTCAG ACTTGGAGTC AGTTTTGCAG 
CAGAAAGACG TGGAGCTCAA GGCTTCAGAA ACCCAGAGAA ACATCTTAGA GCAAGACCTG 
GCAACCTATA TCACCGAATG CAGCAGTTTG AAGCGCAGTT TGGAGCAGGC CCGCATGGAG 
GTTTCGCAGG AAGATGATAA AGCTCTTCAG CTGCTGCACG ACATTCGAGA GCAGAGCAAC 
AAACTGCAGG AAATCAAAGA ACAGGAGTAC CACGCTCAGC TGGAGGAGAT GCGAGTGTCT 
ATTCGTCAGC TGGAAGAGAA TCTGTCGGCC GCTCGTCGCC GCAGTGACCT GTACGAGTCT 
GAGCTCAAAG AGTCCCGTCA GACCAGTGAG GAGCTCAAGA GGAAGGCAGT GGAGTATCAG 
CAGAAGATCC AGAAGGCTAA AGAACAAGGG AAAGTTGAGA TGGAGGAGCT TCTGAGCAAA 
CTGCAGAAGA CCAATGGAGA GCAACAAGTA AAGATTGAAG AGCTCCAGGA GAAGCTTGCT 
AAGGCAGTGA AGGCGAGTAC TGAAGCCACT GAACTCCTCC AGAATGTCCG TCAGGCTAAA 
GAGCGAATGG AGCGAGACCT GGAGCGTCTG CAGACCAAAG AGGACTCGTC CGACAGCCTG 
CGCAGACGCT TACGAGAGAC TGAGGATGGC AAAAAATCCC TGGAGAACCA GGTGAAGAGG 
CTGGAGATGG TGGAGCGGAG AGAATCCAAA CTGAAAGACG ACATTCAGAC CAAATCGCAG 
CAGATCCAGC AGATGGCCGA CAAAATCATG GAGCTTGAAG AAAATTTGCG GGACTCGCAG 
GCGATGGCGC AGCGCATGGA AACACAGCTG GAGCAGAAGG AAAAGCTTTT TGAAGACAAG 
ATCAAGGTTT TAGAAACTCA AATGAAGGCT GATTTGGCTG AGAAGGATTT GCTGGAGTCC 
AAACAGAGCA CTTTTGAAGA GGAGTCTCGC GAACGGGGAA AAGTGATCAG CGACCAGGCA 
GCGACGATCA ATGCCATGGA GTCAAAGATG ACCAGTCTGG AGCAGAGGAT CGCTGAGCTG 
TCCGAAGCCA ACAAACTGGC AGCAAACAGC AGCATTTACA CACAGAAGAA CATGAAAGCC 
CAGGAGGAGA TGATTTCTGA ACTCAGACAG CAGAAGTTTT ATTTGGAGTC TCAGGCTGGA 
AAACTGGAGG CGCAGAATGC AAAGCTGGAG GAGCATCTGG AGAAACTCAC CCAACAAGAG 
CAGACAAACA AGAGCCGAGT GCTGGAGCTG GAAGCCAGGC TGCGAGAGAT CGGCCTAGAG 
CATGAGGAAC AGAAGCTGGA GATCAAGCGT CAGGTGTCGG AGCTCACGTT GTCTCTGCAG 
GAGAGAGAAG CTCAGATCAG CAGCCTGCAA GCGGCACGCC ACGCTCTCGA CAGCCAACTA 
CAGCAGGCCA AAACTGAGCT GGAGGAGACC ACCGCCGAGG CCGAAGAGGA GATCACCGTC 
CTGAGGGCCC ATCGGGATGA AATCCAACGC AAGTTTGATG CCCTGCGAGA CAGCTGTGCG 
GTGATCACGG ATCTGGAGGA GCAGTTGACG CAGCTCACGC AAGAAAACGC GGAGCTGAAC 
CGGCAGAACT TCTACCTGTC CAAACAGCTG GACGAGGCCA CAGACGAGAA CGAGGAGCGC 
CTGCAGCTCA AGCAGGACGT GGAGCGCCTC AGGAGAGAGG TGGCAGACCG AGAGATGCAC 
CTCAACAACC AGAAACAGGT GACACGGCAA CACACACACA CACACACACA CACACACACA 
CACAGGACAG AGGTGGCAGA CCGAGAGATG CACCTCAACA ACCAGAAACA GAACATCGAG 
ACGCTGAAGA CGACATGTAC AATGCTGGAG GAGCAGGTTA TGGAGCTGGA GACTCTGAAT 
GATGAGCTGA TGGAGAAGGA GAGGCAGTGG GAGAACTGGA GATCTGCTCT GGACGAGGAG 
AAAAACCAGG CCGAGCGCAG AGCCAGAGAC CTGCAAAGAC AGCTGGACAG CGAGAAGCAG 
AACCGACTGC GTGCAGAACA GAGAAACTCT GAGTCTCGGC AGGCCGTGGA GCTGGCGGTG 
AAAGAGCACA AAGCTGAAAT CCTGGTTCTG CAGCAGGCGC TCAAAGACCA GAAACTCAAG 
GCTGAGGGCC TTTCTGACAC TCTGGGTGAT CTGGAGAAGA AGCACGGCAT GCTGGAGATG 
AACGCTCGCA GCCTGCAGCA GAAGCTGGAG ACGGAGAGAG AGATGAAGCA GAGACTCATG 
GACGAGCAAG CTAAACTGCA GCAGCAGCTG GACATGCAGA AGAGCCACAT CTTCAGACTG 
ACTCAAGGAC TGCAGGATGC GCTGGACCAG ACCGACCTGC TGAAGACCGA GCGCACAGAC 
CTGGAGTACC AGCTGGAAAA CATACAGGCG GCGTATTCTC ACGAGAAGGT GAAGATGGAG 
GGGACGATCA CCCAGCAGAC CAAGCTCATA GACTTCCTGC AGGCCAAGGT AGAGCACCCC 
TCTAAGAAGA AAAAGGGGCT GTTTGGTGGC CGGCGGCGGG AGGATCTGCT AGCGGCGCTG 
GCGGCTCAGG GACAGACACA GGTGCCTGTG GTGCCCGCCG CCACTCCTGT GCCCCTGCAG 
TACAGCGACC TGAAGGCGGC GCTGGATAAA GAGCGAGCCC GATGCTCCGA GCTGGAGGAC 
GCCCTGCAGA AAACACGCCT GGAGCTCAGA GAAGTTCAGT TTAATAAAGC TCTGGAGCAC 
AGCTCTTCTC CAGCCACTCC AGGTTTGGCC CGTCAGCAGC TCATGATGTC CACCAAGTCC 
AACAAGTCTC CAGAGCACCA GTCCAGCGGC GGCCTGATGT CCTCAAACTC CAGCCGCGGA 
CGCAAAGACA ACGCCAATCC TGAAGAGTAC ACCAGACGTG TGAAGGACCG GATTCATCAC 
AACACTCCTC ACCGCTTCAC GCAGGGGCTC AATATGAGAG CGGCTCGCTG TACTGTGTGT 
CTGGACACAG TCCATTTTGG ACGTCAGGCT GCAACATGCA TTGAATGTCA CGCGTTGTGT 
CACCCAAAAT GCTCCCCGTA CCTCCCTGCC ACCTGCGGCG TTCCCACTGA ATACGCGCTG 
CACCTCTCAG AGGCTCTGTG TCGGGAGAAG GGAAGCACTT CCGGCCTGAA AGATATGGGT 
GGACATATGA GGCTTGAAGG ATGGCTGAAG CAGCCCAGGA ACGGGAAGCG CGGGCAGGGC 
TGGGAGAGGA AGTATGTGGT GCTGGATGGA TATAAAGTGA TTACATTCGA GACAGAACCA 
AGAGACGAGT CAGTGAAGCC GCTGGATGAG TTTGAGCTGT GTCTCTCTGA TGGAGAAGTG 
ACCGTTCATT CTGCTGTGGG TTCCTCTGAG CTCCCCAACA CCGCTAAAAC AGATGTGCCG 
TACGTGCTGA AGCTGGAGTC GTCTCCTCAG TCCACATGCT GGCCCGGTCA GACTCTGTAC 
CTCATGACCT CGGGATACTC AGAGAAGCAG CGCTGGGTGG CAGTGCTGGA AGCCATCGTG 
GCCAGCAGTC GCGGAGCCAG AGAGAAAGCT GAGGCAGATG CTAAATTATT GGGTAACTCT 
TTACTTAAGC TAGAAGGTGA CGACCGTCTG GATATCAACT GCACCCTGCC GCTGACCGAC 
CAGATTGTGT TGGTGGGCTC TGAGGAGGGT CTCTACGCAC TGAATGTGAT TAAGAACTCC 
CTCACTCATA TCCCGGGTCT GGGATCCGTG TTTCAGATCC ACATTATCAA AGATCATGAC 
AAGCTGCTGA TGATCGTGGG AGACGAGCGA GCGCTGTGTC TGGTGGAGAT TAAGAAGGTG 
AAGCAGTCTT TATCCCAGTC TCACCTCCCC ACACTGCCCG AGATTGTGCC GTATATCTTT 
GAGACCGTCA AGGGCTGTCA TCTGTTTGCC TCGGGCAGGA TAGAAAATGG CCCATGCATT 
TGTGCTGCGA TGCCCAATAA AGTCACGATT CTACGATACA ATGACAATCT TAACAAGTTC 
TGCATACGGA AGGAGATTGA AACTCTGGAG CCGTGCAGCT GCATCCACTT CACCAGCTAC 
AGCATCATCA TTGGTACCAA TAAATTCTAC GAGATCGAGA TGAAGCAGTG CGTTCTGGAC 
GAGTTCCTGG ACAAGAATGA CGTGTCGCTG GCGTCGGCTG TCTTCACCGG ATCCTCCCAC 
AGTTTCCCCA TCTCTATCGT CCAGGTGTCC AGCACTCCGC AGAAGGAGGA ATATCTGCTG 
TGTTTCCACG AGATCGGAGT TTTTGTGGAT GCTTACGGTC GAAGAAGTCG CCCTGATGAT 
CTGAAGTGGA GTCGACTTCC CCTGACGTTC GCCTTCAGAG AGCCGTATCT CTTTGTGACC 
TACTTCAACT CTCTGGATGT TATTGAGATT CAGGGACACG CAGCTCTTGG GCCTCCCAGT 
CTGGTTCACC TGGACATTCC TAACCCACGT TATCTGGGTC CAGCCATATC GTCTGGTGCC 
ATCTATCTGG CCTCGTCCTA CCAGAACAAA CTGCGGGTGA TCTGCTGTAA GGGCAGTCTG 
GTGCGGGAGT CTGGAGAGCT GCAGAGGACC GGCTCCAGCA GGAGCCCCAA CAAGCGCGGT 
CCACCAACAT ACAACGAGCA CATCTCCAAA CGTCTGGCCT CGAGTCCTGG AGCCCAGGAG 
GGGCCCCTGC ACCGCGAGCC CAGCACTCCT CACCGCTACC GCGAGGGCCG CACCGAGTTC 
CGGAGAGACA AATCTCCAGC CCGGCCCCTG GAGCGAGAGA AATCCCCGGG CCGTCCGGTG 
CTAGACAGCC GCAGAGAGAG GTCTCCCGGT CGCTTCGAGA GCAGCGGCAG CGAGAGAGAC 
CGCGAGCGGA CACGACTGCA CACTAGCTCT GTACGCACCC AGCTGACCCC CGTCAACAAG 
GTTTGGGACC AGTCTTCGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100333880 ( XM_009303724.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301999.2 Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X9, mRNA.

Price & Availability↑

CloneID	ODa60011
Clone ID Related Accession (Same CDS sequence)	XM_009303724.2 , XM_009303723.2
Accession Version	XM_009303724.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	citron Rho-interacting kinase-like isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303724.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG AGATTTCCAA AGTGCAGCGT AAGATGTCAG ACTTGGAGTC AGTTTTGCAG 
CAGAAAGACG TGGAGCTCAA GGCTTCAGAA ACCCAGAGAA ACATCTTAGA GCAAGACCTG 
GCAACCTATA TCACCGAATG CAGCAGTTTG AAGCGCAGTT TGGAGCAGGC CCGCATGGAG 
GTTTCGCAGG AAGATGATAA AGCTCTTCAG CTGCTGCACG ACATTCGAGA GCAGAGCAAC 
AAACTGCAGG AAATCAAAGA ACAGGAGTAC CACGCTCAGC TGGAGGAGAT GCGAGTGTCT 
ATTCGTCAGC TGGAAGAGAA TCTGTCGGCC GCTCGTCGCC GCAGTGACCT GTACGAGTCT 
GAGCTCAAAG AGTCCCGTCA GACCAGTGAG GAGCTCAAGA GGAAGGCAGT GGAGTATCAG 
CAGAAGATCC AGAAGGCTAA AGAACAAGGG AAAGTTGAGA TGGAGGAGCT TCTGAGCAAA 
CTGCAGAAGA CCAATGGAGA GCAACAAGTA AAGATTGAAG AGCTCCAGGA GAAGCTTGCT 
AAGGCAGTGA AGGCGAGTAC TGAAGCCACT GAACTCCTCC AGAATGTCCG TCAGGCTAAA 
GAGCGAATGG AGCGAGACCT GGAGCGTCTG CAGACCAAAG AGGACTCGTC CGACAGCCTG 
CGCAGACGCT TACGAGAGAC TGAGGATGGC AAAAAATCCC TGGAGAACCA GGTGAAGAGG 
CTGGAGATGG TGGAGCGGAG AGAATCCAAA CTGAAAGACG ACATTCAGAC CAAATCGCAG 
CAGATCCAGC AGATGGCCGA CAAAATCATG GAGCTTGAAG AAAATTTGCG GGACTCGCAG 
GCGATGGCGC AGCGCATGGA AACACAGCTG GAGCAGAAGG AAAAGCTTTT TGAAGACAAG 
ATCAAGGTTT TAGAAACTCA AATGAAGGCT GATTTGGCTG AGAAGGATTT GCTGGAGTCC 
AAACAGAGCA CTTTTGAAGA GGAGTCTCGC GAACGGGGAA AAGTGATCAG CGACCAGGCA 
GCGACGATCA ATGCCATGGA GTCAAAGATG ACCAGTCTGG AGCAGAGGAT CGCTGAGCTG 
TCCGAAGCCA ACAAACTGGC AGCAAACAGC AGCATTTACA CACAGAAGAA CATGAAAGCC 
CAGGAGGAGA TGATTTCTGA ACTCAGACAG CAGAAGTTTT ATTTGGAGTC TCAGGCTGGA 
AAACTGGAGG CGCAGAATGC AAAGCTGGAG GAGCATCTGG AGAAACTCAC CCAACAAGAG 
CAGACAAACA AGAGCCGAGT GCTGGAGCTG GAAGCCAGGC TGCGAGAGAT CGGCCTAGAG 
CATGAGGAAC AGAAGCTGGA GATCAAGCGT CAGGTGTCGG AGCTCACGTT GTCTCTGCAG 
GAGAGAGAAG CTCAGATCAG CAGCCTGCAA GCGGCACGCC ACGCTCTCGA CAGCCAACTA 
CAGCAGGCCA AAACTGAGCT GGAGGAGACC ACCGCCGAGG CCGAAGAGGA GATCACCGTC 
CTGAGGGCCC ATCGGGATGA AATCCAACGC AAGTTTGATG CCCTGCGAGA CAGCTGTGCG 
GTGATCACGG ATCTGGAGGA GCAGTTGACG CAGCTCACGC AAGAAAACGC GGAGCTGAAC 
CGGCAGAACT TCTACCTGTC CAAACAGCTG GACGAGGCCA CAGACGAGAA CGAGGAGCGC 
CTGCAGCTCA AGCAGGACGT GGAGCGCCTC AGGAGAGAGG TGGCAGACCG AGAGATGCAC 
CTCAACAACC AGAAACAGAA CATCGAGACG CTGAAGACGA CATGTACAAT GCTGGAGGAG 
CAGGTTATGG AGCTGGAGAC TCTGAATGAT GAGCTGATGG AGAAGGAGAG GCAGTGGGAG 
AACTGGAGAT CTGCTCTGGA CGAGGAGAAA AACCAGGCCG AGCGCAGAGC CAGAGACCTG 
CAAAGACAGC TGGACAGCGA GAAGCAGAAC CGACTGCGTG CAGAACAGAG AAACTCTGAG 
TCTCGGCAGG CCGTGGAGCT GGCGGTGAAA GAGCACAAAG CTGAAATCCT GGTTCTGCAG 
CAGGCGCTCA AAGACCAGAA ACTCAAGGCT GAGGGCCTTT CTGACACTCT GGGTGATCTG 
GAGAAGAAGC ACGGCATGCT GGAGATGAAC GCTCGCAGCC TGCAGCAGAA GCTGGAGACG 
GAGAGAGAGA TGAAGCAGAG ACTCATGGAC GAGCAAGCTA AACTGCAGCA GCAGCTGGAC 
ATGCAGAAGA GCCACATCTT CAGACTGACT CAAGGACTGC AGGATGCGCT GGACCAGACC 
GACCTGCTGA AGACCGAGCG CACAGACCTG GAGTACCAGC TGGAAAACAT ACAGGCGGCG 
TATTCTCACG AGAAGGTGAA GATGGAGGGG ACGATCACCC AGCAGACCAA GCTCATAGAC 
TTCCTGCAGG CCAAGGTAGA GCACCCCTCT AAGAAGAAAA AGGGGCTGTT TGGTGGCCGG 
CGGCGGGAGG ATCTGCTAGC GGCGCTGGCG GCTCAGGGAC AGACACAGGT GCCTGTGGTG 
CCCGCCGCCA CTCCTGTGCC CCTGCAGTAC AGCGACCTGA AGGCGGCGCT GGATAAAGAG 
CGAGCCCGAT GCTCCGAGCT GGAGGACGCC CTGCAGAAAA CACGCCTGGA GCTCAGAGAA 
GTTCAGTTTA ATAAAGCTCT GGAGCACAGC TCTTCTCCAG CCACTCCAGG TTTGGCCCGT 
CAGCAGCTCA TGATGTCCAC CAAGTCCAAC AAGTCTCCAG AGCACCAGTC CAGCGGCGGC 
CTGATGTCCT CAAACTCCAG CCGCGGACGC AAAGACAACG CCAATCCTGA AGAAATAAGG 
AGAGTCACTT CTGAAAAGTA CACCAGACGT GTGAAGGACC GGATTCATCA CAACACTCCT 
CACCGCTTCA CGCAGGGGCT CAATATGAGA GCGGCTCGCT GTACTGTGTG TCTGGACACA 
GTCCATTTTG GACGTCAGGC TGCAACATGC ATTGAATGTC ACGCGTTGTG TCACCCAAAA 
TGCTCCCCGT ACCTCCCTGC CACCTGCGGC GTTCCCACTG AATACGCGCT GCACCTCTCA 
GAGGCTCTGT GTCGGGAGAA GGGAAGCACT TCCGGCCTGA AAGATATGGG TGGACATATG 
AGGCTTGAAG GATGGCTGAA GCAGCCCAGG AACGGGAAGC GCGGGCAGGG CTGGGAGAGG 
AAGTATGTGG TGCTGGATGG ATATAAAGTG ATTACATTCG AGACAGAACC AAGAGACGAG 
TCAGTGAAGC CGCTGGATGA GTTTGAGCTG TGTCTCTCTG ATGGAGAAGT GACCGTTCAT 
TCTGCTGTGG GTTCCTCTGA GCTCCCCAAC ACCGCTAAAA CAGATGTGCC GTACGTGCTG 
AAGCTGGAGT CGTCTCCTCA GTCCACATGC TGGCCCGGTC AGACTCTGTA CCTCATGACC 
TCGGGATACT CAGAGAAGCA GCGCTGGGTG GCAGTGCTGG AAGCCATCGT GGCCAGCAGT 
CGCGGAGCCA GAGAGAAAGC TGAGGCAGAT GCTAAATTAT TGGGTAACTC TTTACTTAAG 
CTAGAAGGTG ACGACCGTCT GGATATCAAC TGCACCCTGC CGCTGACCGA CCAGATTGTG 
TTGGTGGGCT CTGAGGAGGG TCTCTACGCA CTGAATGTGA TTAAGAACTC CCTCACTCAT 
ATCCCGGGTC TGGGATCCGT GTTTCAGATC CACATTATCA AAGATCATGA CAAGCTGCTG 
ATGATCGTGG GAGACGAGCG AGCGCTGTGT CTGGTGGAGA TTAAGAAGGT GAAGCAGTCT 
TTATCCCAGT CTCACCTCCC CACACTGCCC GAGATTGTGC CGTATATCTT TGAGACCGTC 
AAGGGCTGTC ATCTGTTTGC CTCGGGCAGG ATAGAAAATG GCCCATGCAT TTGTGCTGCG 
ATGCCCAATA AAGTCACGAT TCTACGATAC AATGACAATC TTAACAAGTT CTGCATACGG 
AAGGAGATTG AAACTCTGGA GCCGTGCAGC TGCATCCACT TCACCAGCTA CAGCATCATC 
ATTGGTACCA ATAAATTCTA CGAGATCGAG ATGAAGCAGT GCGTTCTGGA CGAGTTCCTG 
GACAAGAATG ACGTGTCGCT GGCGTCGGCT GTCTTCACCG GATCCTCCCA CAGTTTCCCC 
ATCTCTATCG TCCAGGTGTC CAGCACTCCG CAGAAGGAGG AATATCTGCT GTGTTTCCAC 
GAGATCGGAG TTTTTGTGGA TGCTTACGGT CGAAGAAGTC GCCCTGATGA TCTGAAGTGG 
AGTCGACTTC CCCTGACGTT CGCCTTCAGA GAGCCGTATC TCTTTGTGAC CTACTTCAAC 
TCTCTGGATG TTATTGAGAT TCAGGGACAC GCAGCTCTTG GGCCTCCCAG TCTGGTTCAC 
CTGGACATTC CTAACCCACG TTATCTGGGT CCAGCCATAT CGTCTGGTGC CATCTATCTG 
GCCTCGTCCT ACCAGAACAA ACTGCGGGTG ATCTGCTGTA AGGGCAGTCT GGTGCGGGAG 
TCTGGAGAGC TGCAGAGGAC CGGCTCCAGC AGGAGCAGCC CCAACAAGCG CGGTCCACCA 
ACATACAACG AGCACATCTC CAAACGTCTG GCCTCGAGTC CTGGAGCCCA GGAGGGGCCC 
CTGCACCGCG AGCCCAGCAC TCCTCACCGC TACCGCGAGG GCCGCACCGA GTTCCGGAGA 
GACAAATCTC CAGCCCGGCC CCTGGAGCGA GAGAAATCCC CGGGCCGTCC GGTGCTAGAC 
AGCCGCAGAG AGAGGTCTCC CGGTCGCTTC GAGAGCAGCG GCAGCGAGAG AGACCGCGAG 
CGGACACGAC TGCACACTAG CTCTGTACGC ACCCAGCTGA CCCCCGTCAA CAAGGTTTGG 
GACCAGTCTT CGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301999.2
CDS	434..5311
Translation	MEQEISKVQRKMSDLESVLQQKDVELKASETQRNILEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSNKLQEIKEQEYHAQLEEMRVSIRQLEENLSAARRRSDLYESELKESRQTSEELKRKAVEYQQKIQKAKEQGKVEMEELLSKLQKTNGEQQVKIEELQEKLAKAVKASTEATELLQNVRQAKERMERDLERLQTKEDSSDSLRRRLRETEDGKKSLENQVKRLEMVERRESKLKDDIQTKSQQIQQMADKIMELEENLRDSQAMAQRMETQLEQKEKLFEDKIKVLETQMKADLAEKDLLESKQSTFEEESRERGKVISDQAATINAMESKMTSLEQRIAELSEANKLAANSSIYTQKNMKAQEEMISELRQQKFYLESQAGKLEAQNAKLEEHLEKLTQQEQTNKSRVLELEARLREIGLEHEEQKLEIKRQVSELTLSLQEREAQISSLQAARHALDSQLQQAKTELEETTAEAEEEITVLRAHRDEIQRKFDALRDSCAVITDLEEQLTQLTQENAELNRQNFYLSKQLDEATDENEERLQLKQDVERLRREVADREMHLNNQKQNIETLKTTCTMLEEQVMELETLNDELMEKERQWENWRSALDEEKNQAERRARDLQRQLDSEKQNRLRAEQRNSESRQAVELAVKEHKAEILVLQQALKDQKLKAEGLSDTLGDLEKKHGMLEMNARSLQQKLETEREMKQRLMDEQAKLQQQLDMQKSHIFRLTQGLQDALDQTDLLKTERTDLEYQLENIQAAYSHEKVKMEGTITQQTKLIDFLQAKVEHPSKKKKGLFGGRRREDLLAALAAQGQTQVPVVPAATPVPLQYSDLKAALDKERARCSELEDALQKTRLELREVQFNKALEHSSSPATPGLARQQLMMSTKSNKSPEHQSSGGLMSSNSSRGRKDNANPEEIRRVTSEKYTRRVKDRIHHNTPHRFTQGLNMRAARCTVCLDTVHFGRQAATCIECHALCHPKCSPYLPATCGVPTEYALHLSEALCREKGSTSGLKDMGGHMRLEGWLKQPRNGKRGQGWERKYVVLDGYKVITFETEPRDESVKPLDEFELCLSDGEVTVHSAVGSSELPNTAKTDVPYVLKLESSPQSTCWPGQTLYLMTSGYSEKQRWVAVLEAIVASSRGAREKAEADAKLLGNSLLKLEGDDRLDINCTLPLTDQIVLVGSEEGLYALNVIKNSLTHIPGLGSVFQIHIIKDHDKLLMIVGDERALCLVEIKKVKQSLSQSHLPTLPEIVPYIFETVKGCHLFASGRIENGPCICAAMPNKVTILRYNDNLNKFCIRKEIETLEPCSCIHFTSYSIIIGTNKFYEIEMKQCVLDEFLDKNDVSLASAVFTGSSHSFPISIVQVSSTPQKEEYLLCFHEIGVFVDAYGRRSRPDDLKWSRLPLTFAFREPYLFVTYFNSLDVIEIQGHAALGPPSLVHLDIPNPRYLGPAISSGAIYLASSYQNKLRVICCKGSLVRESGELQRTGSSRSSPNKRGPPTYNEHISKRLASSPGAQEGPLHREPSTPHRYREGRTEFRRDKSPARPLEREKSPGRPVLDSRRERSPGRFESSGSERDRERTRLHTSSVRTQLTPVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_009303724.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303724.2

ORF Insert Sequence:

ATGGAGCAGG AGATTTCCAA AGTGCAGCGT AAGATGTCAG ACTTGGAGTC AGTTTTGCAG 
CAGAAAGACG TGGAGCTCAA GGCTTCAGAA ACCCAGAGAA ACATCTTAGA GCAAGACCTG 
GCAACCTATA TCACCGAATG CAGCAGTTTG AAGCGCAGTT TGGAGCAGGC CCGCATGGAG 
GTTTCGCAGG AAGATGATAA AGCTCTTCAG CTGCTGCACG ACATTCGAGA GCAGAGCAAC 
AAACTGCAGG AAATCAAAGA ACAGGAGTAC CACGCTCAGC TGGAGGAGAT GCGAGTGTCT 
ATTCGTCAGC TGGAAGAGAA TCTGTCGGCC GCTCGTCGCC GCAGTGACCT GTACGAGTCT 
GAGCTCAAAG AGTCCCGTCA GACCAGTGAG GAGCTCAAGA GGAAGGCAGT GGAGTATCAG 
CAGAAGATCC AGAAGGCTAA AGAACAAGGG AAAGTTGAGA TGGAGGAGCT TCTGAGCAAA 
CTGCAGAAGA CCAATGGAGA GCAACAAGTA AAGATTGAAG AGCTCCAGGA GAAGCTTGCT 
AAGGCAGTGA AGGCGAGTAC TGAAGCCACT GAACTCCTCC AGAATGTCCG TCAGGCTAAA 
GAGCGAATGG AGCGAGACCT GGAGCGTCTG CAGACCAAAG AGGACTCGTC CGACAGCCTG 
CGCAGACGCT TACGAGAGAC TGAGGATGGC AAAAAATCCC TGGAGAACCA GGTGAAGAGG 
CTGGAGATGG TGGAGCGGAG AGAATCCAAA CTGAAAGACG ACATTCAGAC CAAATCGCAG 
CAGATCCAGC AGATGGCCGA CAAAATCATG GAGCTTGAAG AAAATTTGCG GGACTCGCAG 
GCGATGGCGC AGCGCATGGA AACACAGCTG GAGCAGAAGG AAAAGCTTTT TGAAGACAAG 
ATCAAGGTTT TAGAAACTCA AATGAAGGCT GATTTGGCTG AGAAGGATTT GCTGGAGTCC 
AAACAGAGCA CTTTTGAAGA GGAGTCTCGC GAACGGGGAA AAGTGATCAG CGACCAGGCA 
GCGACGATCA ATGCCATGGA GTCAAAGATG ACCAGTCTGG AGCAGAGGAT CGCTGAGCTG 
TCCGAAGCCA ACAAACTGGC AGCAAACAGC AGCATTTACA CACAGAAGAA CATGAAAGCC 
CAGGAGGAGA TGATTTCTGA ACTCAGACAG CAGAAGTTTT ATTTGGAGTC TCAGGCTGGA 
AAACTGGAGG CGCAGAATGC AAAGCTGGAG GAGCATCTGG AGAAACTCAC CCAACAAGAG 
CAGACAAACA AGAGCCGAGT GCTGGAGCTG GAAGCCAGGC TGCGAGAGAT CGGCCTAGAG 
CATGAGGAAC AGAAGCTGGA GATCAAGCGT CAGGTGTCGG AGCTCACGTT GTCTCTGCAG 
GAGAGAGAAG CTCAGATCAG CAGCCTGCAA GCGGCACGCC ACGCTCTCGA CAGCCAACTA 
CAGCAGGCCA AAACTGAGCT GGAGGAGACC ACCGCCGAGG CCGAAGAGGA GATCACCGTC 
CTGAGGGCCC ATCGGGATGA AATCCAACGC AAGTTTGATG CCCTGCGAGA CAGCTGTGCG 
GTGATCACGG ATCTGGAGGA GCAGTTGACG CAGCTCACGC AAGAAAACGC GGAGCTGAAC 
CGGCAGAACT TCTACCTGTC CAAACAGCTG GACGAGGCCA CAGACGAGAA CGAGGAGCGC 
CTGCAGCTCA AGCAGGACGT GGAGCGCCTC AGGAGAGAGG TGGCAGACCG AGAGATGCAC 
CTCAACAACC AGAAACAGAA CATCGAGACG CTGAAGACGA CATGTACAAT GCTGGAGGAG 
CAGGTTATGG AGCTGGAGAC TCTGAATGAT GAGCTGATGG AGAAGGAGAG GCAGTGGGAG 
AACTGGAGAT CTGCTCTGGA CGAGGAGAAA AACCAGGCCG AGCGCAGAGC CAGAGACCTG 
CAAAGACAGC TGGACAGCGA GAAGCAGAAC CGACTGCGTG CAGAACAGAG AAACTCTGAG 
TCTCGGCAGG CCGTGGAGCT GGCGGTGAAA GAGCACAAAG CTGAAATCCT GGTTCTGCAG 
CAGGCGCTCA AAGACCAGAA ACTCAAGGCT GAGGGCCTTT CTGACACTCT GGGTGATCTG 
GAGAAGAAGC ACGGCATGCT GGAGATGAAC GCTCGCAGCC TGCAGCAGAA GCTGGAGACG 
GAGAGAGAGA TGAAGCAGAG ACTCATGGAC GAGCAAGCTA AACTGCAGCA GCAGCTGGAC 
ATGCAGAAGA GCCACATCTT CAGACTGACT CAAGGACTGC AGGATGCGCT GGACCAGACC 
GACCTGCTGA AGACCGAGCG CACAGACCTG GAGTACCAGC TGGAAAACAT ACAGGCGGCG 
TATTCTCACG AGAAGGTGAA GATGGAGGGG ACGATCACCC AGCAGACCAA GCTCATAGAC 
TTCCTGCAGG CCAAGGTAGA GCACCCCTCT AAGAAGAAAA AGGGGCTGTT TGGTGGCCGG 
CGGCGGGAGG ATCTGCTAGC GGCGCTGGCG GCTCAGGGAC AGACACAGGT GCCTGTGGTG 
CCCGCCGCCA CTCCTGTGCC CCTGCAGTAC AGCGACCTGA AGGCGGCGCT GGATAAAGAG 
CGAGCCCGAT GCTCCGAGCT GGAGGACGCC CTGCAGAAAA CACGCCTGGA GCTCAGAGAA 
GTTCAGTTTA ATAAAGCTCT GGAGCACAGC TCTTCTCCAG CCACTCCAGG TTTGGCCCGT 
CAGCAGCTCA TGATGTCCAC CAAGTCCAAC AAGTCTCCAG AGCACCAGTC CAGCGGCGGC 
CTGATGTCCT CAAACTCCAG CCGCGGACGC AAAGACAACG CCAATCCTGA AGAAATAAGG 
AGAGTCACTT CTGAAAAGTA CACCAGACGT GTGAAGGACC GGATTCATCA CAACACTCCT 
CACCGCTTCA CGCAGGGGCT CAATATGAGA GCGGCTCGCT GTACTGTGTG TCTGGACACA 
GTCCATTTTG GACGTCAGGC TGCAACATGC ATTGAATGTC ACGCGTTGTG TCACCCAAAA 
TGCTCCCCGT ACCTCCCTGC CACCTGCGGC GTTCCCACTG AATACGCGCT GCACCTCTCA 
GAGGCTCTGT GTCGGGAGAA GGGAAGCACT TCCGGCCTGA AAGATATGGG TGGACATATG 
AGGCTTGAAG GATGGCTGAA GCAGCCCAGG AACGGGAAGC GCGGGCAGGG CTGGGAGAGG 
AAGTATGTGG TGCTGGATGG ATATAAAGTG ATTACATTCG AGACAGAACC AAGAGACGAG 
TCAGTGAAGC CGCTGGATGA GTTTGAGCTG TGTCTCTCTG ATGGAGAAGT GACCGTTCAT 
TCTGCTGTGG GTTCCTCTGA GCTCCCCAAC ACCGCTAAAA CAGATGTGCC GTACGTGCTG 
AAGCTGGAGT CGTCTCCTCA GTCCACATGC TGGCCCGGTC AGACTCTGTA CCTCATGACC 
TCGGGATACT CAGAGAAGCA GCGCTGGGTG GCAGTGCTGG AAGCCATCGT GGCCAGCAGT 
CGCGGAGCCA GAGAGAAAGC TGAGGCAGAT GCTAAATTAT TGGGTAACTC TTTACTTAAG 
CTAGAAGGTG ACGACCGTCT GGATATCAAC TGCACCCTGC CGCTGACCGA CCAGATTGTG 
TTGGTGGGCT CTGAGGAGGG TCTCTACGCA CTGAATGTGA TTAAGAACTC CCTCACTCAT 
ATCCCGGGTC TGGGATCCGT GTTTCAGATC CACATTATCA AAGATCATGA CAAGCTGCTG 
ATGATCGTGG GAGACGAGCG AGCGCTGTGT CTGGTGGAGA TTAAGAAGGT GAAGCAGTCT 
TTATCCCAGT CTCACCTCCC CACACTGCCC GAGATTGTGC CGTATATCTT TGAGACCGTC 
AAGGGCTGTC ATCTGTTTGC CTCGGGCAGG ATAGAAAATG GCCCATGCAT TTGTGCTGCG 
ATGCCCAATA AAGTCACGAT TCTACGATAC AATGACAATC TTAACAAGTT CTGCATACGG 
AAGGAGATTG AAACTCTGGA GCCGTGCAGC TGCATCCACT TCACCAGCTA CAGCATCATC 
ATTGGTACCA ATAAATTCTA CGAGATCGAG ATGAAGCAGT GCGTTCTGGA CGAGTTCCTG 
GACAAGAATG ACGTGTCGCT GGCGTCGGCT GTCTTCACCG GATCCTCCCA CAGTTTCCCC 
ATCTCTATCG TCCAGGTGTC CAGCACTCCG CAGAAGGAGG AATATCTGCT GTGTTTCCAC 
GAGATCGGAG TTTTTGTGGA TGCTTACGGT CGAAGAAGTC GCCCTGATGA TCTGAAGTGG 
AGTCGACTTC CCCTGACGTT CGCCTTCAGA GAGCCGTATC TCTTTGTGAC CTACTTCAAC 
TCTCTGGATG TTATTGAGAT TCAGGGACAC GCAGCTCTTG GGCCTCCCAG TCTGGTTCAC 
CTGGACATTC CTAACCCACG TTATCTGGGT CCAGCCATAT CGTCTGGTGC CATCTATCTG 
GCCTCGTCCT ACCAGAACAA ACTGCGGGTG ATCTGCTGTA AGGGCAGTCT GGTGCGGGAG 
TCTGGAGAGC TGCAGAGGAC CGGCTCCAGC AGGAGCAGCC CCAACAAGCG CGGTCCACCA 
ACATACAACG AGCACATCTC CAAACGTCTG GCCTCGAGTC CTGGAGCCCA GGAGGGGCCC 
CTGCACCGCG AGCCCAGCAC TCCTCACCGC TACCGCGAGG GCCGCACCGA GTTCCGGAGA 
GACAAATCTC CAGCCCGGCC CCTGGAGCGA GAGAAATCCC CGGGCCGTCC GGTGCTAGAC 
AGCCGCAGAG AGAGGTCTCC CGGTCGCTTC GAGAGCAGCG GCAGCGAGAG AGACCGCGAG 
CGGACACGAC TGCACACTAG CTCTGTACGC ACCCAGCTGA CCCCCGTCAA CAAGGTTTGG 
GACCAGTCTT CGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100333880 ( XM_009303723.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301998.2 Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X8, mRNA.

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CloneID	ODa60011
Clone ID Related Accession (Same CDS sequence)	XM_009303724.2 , XM_009303723.2
Accession Version	XM_009303723.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	citron Rho-interacting kinase-like isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303723.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG AGATTTCCAA AGTGCAGCGT AAGATGTCAG ACTTGGAGTC AGTTTTGCAG 
CAGAAAGACG TGGAGCTCAA GGCTTCAGAA ACCCAGAGAA ACATCTTAGA GCAAGACCTG 
GCAACCTATA TCACCGAATG CAGCAGTTTG AAGCGCAGTT TGGAGCAGGC CCGCATGGAG 
GTTTCGCAGG AAGATGATAA AGCTCTTCAG CTGCTGCACG ACATTCGAGA GCAGAGCAAC 
AAACTGCAGG AAATCAAAGA ACAGGAGTAC CACGCTCAGC TGGAGGAGAT GCGAGTGTCT 
ATTCGTCAGC TGGAAGAGAA TCTGTCGGCC GCTCGTCGCC GCAGTGACCT GTACGAGTCT 
GAGCTCAAAG AGTCCCGTCA GACCAGTGAG GAGCTCAAGA GGAAGGCAGT GGAGTATCAG 
CAGAAGATCC AGAAGGCTAA AGAACAAGGG AAAGTTGAGA TGGAGGAGCT TCTGAGCAAA 
CTGCAGAAGA CCAATGGAGA GCAACAAGTA AAGATTGAAG AGCTCCAGGA GAAGCTTGCT 
AAGGCAGTGA AGGCGAGTAC TGAAGCCACT GAACTCCTCC AGAATGTCCG TCAGGCTAAA 
GAGCGAATGG AGCGAGACCT GGAGCGTCTG CAGACCAAAG AGGACTCGTC CGACAGCCTG 
CGCAGACGCT TACGAGAGAC TGAGGATGGC AAAAAATCCC TGGAGAACCA GGTGAAGAGG 
CTGGAGATGG TGGAGCGGAG AGAATCCAAA CTGAAAGACG ACATTCAGAC CAAATCGCAG 
CAGATCCAGC AGATGGCCGA CAAAATCATG GAGCTTGAAG AAAATTTGCG GGACTCGCAG 
GCGATGGCGC AGCGCATGGA AACACAGCTG GAGCAGAAGG AAAAGCTTTT TGAAGACAAG 
ATCAAGGTTT TAGAAACTCA AATGAAGGCT GATTTGGCTG AGAAGGATTT GCTGGAGTCC 
AAACAGAGCA CTTTTGAAGA GGAGTCTCGC GAACGGGGAA AAGTGATCAG CGACCAGGCA 
GCGACGATCA ATGCCATGGA GTCAAAGATG ACCAGTCTGG AGCAGAGGAT CGCTGAGCTG 
TCCGAAGCCA ACAAACTGGC AGCAAACAGC AGCATTTACA CACAGAAGAA CATGAAAGCC 
CAGGAGGAGA TGATTTCTGA ACTCAGACAG CAGAAGTTTT ATTTGGAGTC TCAGGCTGGA 
AAACTGGAGG CGCAGAATGC AAAGCTGGAG GAGCATCTGG AGAAACTCAC CCAACAAGAG 
CAGACAAACA AGAGCCGAGT GCTGGAGCTG GAAGCCAGGC TGCGAGAGAT CGGCCTAGAG 
CATGAGGAAC AGAAGCTGGA GATCAAGCGT CAGGTGTCGG AGCTCACGTT GTCTCTGCAG 
GAGAGAGAAG CTCAGATCAG CAGCCTGCAA GCGGCACGCC ACGCTCTCGA CAGCCAACTA 
CAGCAGGCCA AAACTGAGCT GGAGGAGACC ACCGCCGAGG CCGAAGAGGA GATCACCGTC 
CTGAGGGCCC ATCGGGATGA AATCCAACGC AAGTTTGATG CCCTGCGAGA CAGCTGTGCG 
GTGATCACGG ATCTGGAGGA GCAGTTGACG CAGCTCACGC AAGAAAACGC GGAGCTGAAC 
CGGCAGAACT TCTACCTGTC CAAACAGCTG GACGAGGCCA CAGACGAGAA CGAGGAGCGC 
CTGCAGCTCA AGCAGGACGT GGAGCGCCTC AGGAGAGAGG TGGCAGACCG AGAGATGCAC 
CTCAACAACC AGAAACAGAA CATCGAGACG CTGAAGACGA CATGTACAAT GCTGGAGGAG 
CAGGTTATGG AGCTGGAGAC TCTGAATGAT GAGCTGATGG AGAAGGAGAG GCAGTGGGAG 
AACTGGAGAT CTGCTCTGGA CGAGGAGAAA AACCAGGCCG AGCGCAGAGC CAGAGACCTG 
CAAAGACAGC TGGACAGCGA GAAGCAGAAC CGACTGCGTG CAGAACAGAG AAACTCTGAG 
TCTCGGCAGG CCGTGGAGCT GGCGGTGAAA GAGCACAAAG CTGAAATCCT GGTTCTGCAG 
CAGGCGCTCA AAGACCAGAA ACTCAAGGCT GAGGGCCTTT CTGACACTCT GGGTGATCTG 
GAGAAGAAGC ACGGCATGCT GGAGATGAAC GCTCGCAGCC TGCAGCAGAA GCTGGAGACG 
GAGAGAGAGA TGAAGCAGAG ACTCATGGAC GAGCAAGCTA AACTGCAGCA GCAGCTGGAC 
ATGCAGAAGA GCCACATCTT CAGACTGACT CAAGGACTGC AGGATGCGCT GGACCAGACC 
GACCTGCTGA AGACCGAGCG CACAGACCTG GAGTACCAGC TGGAAAACAT ACAGGCGGCG 
TATTCTCACG AGAAGGTGAA GATGGAGGGG ACGATCACCC AGCAGACCAA GCTCATAGAC 
TTCCTGCAGG CCAAGGTAGA GCACCCCTCT AAGAAGAAAA AGGGGCTGTT TGGTGGCCGG 
CGGCGGGAGG ATCTGCTAGC GGCGCTGGCG GCTCAGGGAC AGACACAGGT GCCTGTGGTG 
CCCGCCGCCA CTCCTGTGCC CCTGCAGTAC AGCGACCTGA AGGCGGCGCT GGATAAAGAG 
CGAGCCCGAT GCTCCGAGCT GGAGGACGCC CTGCAGAAAA CACGCCTGGA GCTCAGAGAA 
GTTCAGTTTA ATAAAGCTCT GGAGCACAGC TCTTCTCCAG CCACTCCAGG TTTGGCCCGT 
CAGCAGCTCA TGATGTCCAC CAAGTCCAAC AAGTCTCCAG AGCACCAGTC CAGCGGCGGC 
CTGATGTCCT CAAACTCCAG CCGCGGACGC AAAGACAACG CCAATCCTGA AGAAATAAGG 
AGAGTCACTT CTGAAAAGTA CACCAGACGT GTGAAGGACC GGATTCATCA CAACACTCCT 
CACCGCTTCA CGCAGGGGCT CAATATGAGA GCGGCTCGCT GTACTGTGTG TCTGGACACA 
GTCCATTTTG GACGTCAGGC TGCAACATGC ATTGAATGTC ACGCGTTGTG TCACCCAAAA 
TGCTCCCCGT ACCTCCCTGC CACCTGCGGC GTTCCCACTG AATACGCGCT GCACCTCTCA 
GAGGCTCTGT GTCGGGAGAA GGGAAGCACT TCCGGCCTGA AAGATATGGG TGGACATATG 
AGGCTTGAAG GATGGCTGAA GCAGCCCAGG AACGGGAAGC GCGGGCAGGG CTGGGAGAGG 
AAGTATGTGG TGCTGGATGG ATATAAAGTG ATTACATTCG AGACAGAACC AAGAGACGAG 
TCAGTGAAGC CGCTGGATGA GTTTGAGCTG TGTCTCTCTG ATGGAGAAGT GACCGTTCAT 
TCTGCTGTGG GTTCCTCTGA GCTCCCCAAC ACCGCTAAAA CAGATGTGCC GTACGTGCTG 
AAGCTGGAGT CGTCTCCTCA GTCCACATGC TGGCCCGGTC AGACTCTGTA CCTCATGACC 
TCGGGATACT CAGAGAAGCA GCGCTGGGTG GCAGTGCTGG AAGCCATCGT GGCCAGCAGT 
CGCGGAGCCA GAGAGAAAGC TGAGGCAGAT GCTAAATTAT TGGGTAACTC TTTACTTAAG 
CTAGAAGGTG ACGACCGTCT GGATATCAAC TGCACCCTGC CGCTGACCGA CCAGATTGTG 
TTGGTGGGCT CTGAGGAGGG TCTCTACGCA CTGAATGTGA TTAAGAACTC CCTCACTCAT 
ATCCCGGGTC TGGGATCCGT GTTTCAGATC CACATTATCA AAGATCATGA CAAGCTGCTG 
ATGATCGTGG GAGACGAGCG AGCGCTGTGT CTGGTGGAGA TTAAGAAGGT GAAGCAGTCT 
TTATCCCAGT CTCACCTCCC CACACTGCCC GAGATTGTGC CGTATATCTT TGAGACCGTC 
AAGGGCTGTC ATCTGTTTGC CTCGGGCAGG ATAGAAAATG GCCCATGCAT TTGTGCTGCG 
ATGCCCAATA AAGTCACGAT TCTACGATAC AATGACAATC TTAACAAGTT CTGCATACGG 
AAGGAGATTG AAACTCTGGA GCCGTGCAGC TGCATCCACT TCACCAGCTA CAGCATCATC 
ATTGGTACCA ATAAATTCTA CGAGATCGAG ATGAAGCAGT GCGTTCTGGA CGAGTTCCTG 
GACAAGAATG ACGTGTCGCT GGCGTCGGCT GTCTTCACCG GATCCTCCCA CAGTTTCCCC 
ATCTCTATCG TCCAGGTGTC CAGCACTCCG CAGAAGGAGG AATATCTGCT GTGTTTCCAC 
GAGATCGGAG TTTTTGTGGA TGCTTACGGT CGAAGAAGTC GCCCTGATGA TCTGAAGTGG 
AGTCGACTTC CCCTGACGTT CGCCTTCAGA GAGCCGTATC TCTTTGTGAC CTACTTCAAC 
TCTCTGGATG TTATTGAGAT TCAGGGACAC GCAGCTCTTG GGCCTCCCAG TCTGGTTCAC 
CTGGACATTC CTAACCCACG TTATCTGGGT CCAGCCATAT CGTCTGGTGC CATCTATCTG 
GCCTCGTCCT ACCAGAACAA ACTGCGGGTG ATCTGCTGTA AGGGCAGTCT GGTGCGGGAG 
TCTGGAGAGC TGCAGAGGAC CGGCTCCAGC AGGAGCAGCC CCAACAAGCG CGGTCCACCA 
ACATACAACG AGCACATCTC CAAACGTCTG GCCTCGAGTC CTGGAGCCCA GGAGGGGCCC 
CTGCACCGCG AGCCCAGCAC TCCTCACCGC TACCGCGAGG GCCGCACCGA GTTCCGGAGA 
GACAAATCTC CAGCCCGGCC CCTGGAGCGA GAGAAATCCC CGGGCCGTCC GGTGCTAGAC 
AGCCGCAGAG AGAGGTCTCC CGGTCGCTTC GAGAGCAGCG GCAGCGAGAG AGACCGCGAG 
CGGACACGAC TGCACACTAG CTCTGTACGC ACCCAGCTGA CCCCCGTCAA CAAGGTTTGG 
GACCAGTCTT CGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301998.2
CDS	434..5311
Translation	MEQEISKVQRKMSDLESVLQQKDVELKASETQRNILEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSNKLQEIKEQEYHAQLEEMRVSIRQLEENLSAARRRSDLYESELKESRQTSEELKRKAVEYQQKIQKAKEQGKVEMEELLSKLQKTNGEQQVKIEELQEKLAKAVKASTEATELLQNVRQAKERMERDLERLQTKEDSSDSLRRRLRETEDGKKSLENQVKRLEMVERRESKLKDDIQTKSQQIQQMADKIMELEENLRDSQAMAQRMETQLEQKEKLFEDKIKVLETQMKADLAEKDLLESKQSTFEEESRERGKVISDQAATINAMESKMTSLEQRIAELSEANKLAANSSIYTQKNMKAQEEMISELRQQKFYLESQAGKLEAQNAKLEEHLEKLTQQEQTNKSRVLELEARLREIGLEHEEQKLEIKRQVSELTLSLQEREAQISSLQAARHALDSQLQQAKTELEETTAEAEEEITVLRAHRDEIQRKFDALRDSCAVITDLEEQLTQLTQENAELNRQNFYLSKQLDEATDENEERLQLKQDVERLRREVADREMHLNNQKQNIETLKTTCTMLEEQVMELETLNDELMEKERQWENWRSALDEEKNQAERRARDLQRQLDSEKQNRLRAEQRNSESRQAVELAVKEHKAEILVLQQALKDQKLKAEGLSDTLGDLEKKHGMLEMNARSLQQKLETEREMKQRLMDEQAKLQQQLDMQKSHIFRLTQGLQDALDQTDLLKTERTDLEYQLENIQAAYSHEKVKMEGTITQQTKLIDFLQAKVEHPSKKKKGLFGGRRREDLLAALAAQGQTQVPVVPAATPVPLQYSDLKAALDKERARCSELEDALQKTRLELREVQFNKALEHSSSPATPGLARQQLMMSTKSNKSPEHQSSGGLMSSNSSRGRKDNANPEEIRRVTSEKYTRRVKDRIHHNTPHRFTQGLNMRAARCTVCLDTVHFGRQAATCIECHALCHPKCSPYLPATCGVPTEYALHLSEALCREKGSTSGLKDMGGHMRLEGWLKQPRNGKRGQGWERKYVVLDGYKVITFETEPRDESVKPLDEFELCLSDGEVTVHSAVGSSELPNTAKTDVPYVLKLESSPQSTCWPGQTLYLMTSGYSEKQRWVAVLEAIVASSRGAREKAEADAKLLGNSLLKLEGDDRLDINCTLPLTDQIVLVGSEEGLYALNVIKNSLTHIPGLGSVFQIHIIKDHDKLLMIVGDERALCLVEIKKVKQSLSQSHLPTLPEIVPYIFETVKGCHLFASGRIENGPCICAAMPNKVTILRYNDNLNKFCIRKEIETLEPCSCIHFTSYSIIIGTNKFYEIEMKQCVLDEFLDKNDVSLASAVFTGSSHSFPISIVQVSSTPQKEEYLLCFHEIGVFVDAYGRRSRPDDLKWSRLPLTFAFREPYLFVTYFNSLDVIEIQGHAALGPPSLVHLDIPNPRYLGPAISSGAIYLASSYQNKLRVICCKGSLVRESGELQRTGSSRSSPNKRGPPTYNEHISKRLASSPGAQEGPLHREPSTPHRYREGRTEFRRDKSPARPLEREKSPGRPVLDSRRERSPGRFESSGSERDRERTRLHTSSVRTQLTPVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_009303723.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303723.2

ORF Insert Sequence:

ATGGAGCAGG AGATTTCCAA AGTGCAGCGT AAGATGTCAG ACTTGGAGTC AGTTTTGCAG 
CAGAAAGACG TGGAGCTCAA GGCTTCAGAA ACCCAGAGAA ACATCTTAGA GCAAGACCTG 
GCAACCTATA TCACCGAATG CAGCAGTTTG AAGCGCAGTT TGGAGCAGGC CCGCATGGAG 
GTTTCGCAGG AAGATGATAA AGCTCTTCAG CTGCTGCACG ACATTCGAGA GCAGAGCAAC 
AAACTGCAGG AAATCAAAGA ACAGGAGTAC CACGCTCAGC TGGAGGAGAT GCGAGTGTCT 
ATTCGTCAGC TGGAAGAGAA TCTGTCGGCC GCTCGTCGCC GCAGTGACCT GTACGAGTCT 
GAGCTCAAAG AGTCCCGTCA GACCAGTGAG GAGCTCAAGA GGAAGGCAGT GGAGTATCAG 
CAGAAGATCC AGAAGGCTAA AGAACAAGGG AAAGTTGAGA TGGAGGAGCT TCTGAGCAAA 
CTGCAGAAGA CCAATGGAGA GCAACAAGTA AAGATTGAAG AGCTCCAGGA GAAGCTTGCT 
AAGGCAGTGA AGGCGAGTAC TGAAGCCACT GAACTCCTCC AGAATGTCCG TCAGGCTAAA 
GAGCGAATGG AGCGAGACCT GGAGCGTCTG CAGACCAAAG AGGACTCGTC CGACAGCCTG 
CGCAGACGCT TACGAGAGAC TGAGGATGGC AAAAAATCCC TGGAGAACCA GGTGAAGAGG 
CTGGAGATGG TGGAGCGGAG AGAATCCAAA CTGAAAGACG ACATTCAGAC CAAATCGCAG 
CAGATCCAGC AGATGGCCGA CAAAATCATG GAGCTTGAAG AAAATTTGCG GGACTCGCAG 
GCGATGGCGC AGCGCATGGA AACACAGCTG GAGCAGAAGG AAAAGCTTTT TGAAGACAAG 
ATCAAGGTTT TAGAAACTCA AATGAAGGCT GATTTGGCTG AGAAGGATTT GCTGGAGTCC 
AAACAGAGCA CTTTTGAAGA GGAGTCTCGC GAACGGGGAA AAGTGATCAG CGACCAGGCA 
GCGACGATCA ATGCCATGGA GTCAAAGATG ACCAGTCTGG AGCAGAGGAT CGCTGAGCTG 
TCCGAAGCCA ACAAACTGGC AGCAAACAGC AGCATTTACA CACAGAAGAA CATGAAAGCC 
CAGGAGGAGA TGATTTCTGA ACTCAGACAG CAGAAGTTTT ATTTGGAGTC TCAGGCTGGA 
AAACTGGAGG CGCAGAATGC AAAGCTGGAG GAGCATCTGG AGAAACTCAC CCAACAAGAG 
CAGACAAACA AGAGCCGAGT GCTGGAGCTG GAAGCCAGGC TGCGAGAGAT CGGCCTAGAG 
CATGAGGAAC AGAAGCTGGA GATCAAGCGT CAGGTGTCGG AGCTCACGTT GTCTCTGCAG 
GAGAGAGAAG CTCAGATCAG CAGCCTGCAA GCGGCACGCC ACGCTCTCGA CAGCCAACTA 
CAGCAGGCCA AAACTGAGCT GGAGGAGACC ACCGCCGAGG CCGAAGAGGA GATCACCGTC 
CTGAGGGCCC ATCGGGATGA AATCCAACGC AAGTTTGATG CCCTGCGAGA CAGCTGTGCG 
GTGATCACGG ATCTGGAGGA GCAGTTGACG CAGCTCACGC AAGAAAACGC GGAGCTGAAC 
CGGCAGAACT TCTACCTGTC CAAACAGCTG GACGAGGCCA CAGACGAGAA CGAGGAGCGC 
CTGCAGCTCA AGCAGGACGT GGAGCGCCTC AGGAGAGAGG TGGCAGACCG AGAGATGCAC 
CTCAACAACC AGAAACAGAA CATCGAGACG CTGAAGACGA CATGTACAAT GCTGGAGGAG 
CAGGTTATGG AGCTGGAGAC TCTGAATGAT GAGCTGATGG AGAAGGAGAG GCAGTGGGAG 
AACTGGAGAT CTGCTCTGGA CGAGGAGAAA AACCAGGCCG AGCGCAGAGC CAGAGACCTG 
CAAAGACAGC TGGACAGCGA GAAGCAGAAC CGACTGCGTG CAGAACAGAG AAACTCTGAG 
TCTCGGCAGG CCGTGGAGCT GGCGGTGAAA GAGCACAAAG CTGAAATCCT GGTTCTGCAG 
CAGGCGCTCA AAGACCAGAA ACTCAAGGCT GAGGGCCTTT CTGACACTCT GGGTGATCTG 
GAGAAGAAGC ACGGCATGCT GGAGATGAAC GCTCGCAGCC TGCAGCAGAA GCTGGAGACG 
GAGAGAGAGA TGAAGCAGAG ACTCATGGAC GAGCAAGCTA AACTGCAGCA GCAGCTGGAC 
ATGCAGAAGA GCCACATCTT CAGACTGACT CAAGGACTGC AGGATGCGCT GGACCAGACC 
GACCTGCTGA AGACCGAGCG CACAGACCTG GAGTACCAGC TGGAAAACAT ACAGGCGGCG 
TATTCTCACG AGAAGGTGAA GATGGAGGGG ACGATCACCC AGCAGACCAA GCTCATAGAC 
TTCCTGCAGG CCAAGGTAGA GCACCCCTCT AAGAAGAAAA AGGGGCTGTT TGGTGGCCGG 
CGGCGGGAGG ATCTGCTAGC GGCGCTGGCG GCTCAGGGAC AGACACAGGT GCCTGTGGTG 
CCCGCCGCCA CTCCTGTGCC CCTGCAGTAC AGCGACCTGA AGGCGGCGCT GGATAAAGAG 
CGAGCCCGAT GCTCCGAGCT GGAGGACGCC CTGCAGAAAA CACGCCTGGA GCTCAGAGAA 
GTTCAGTTTA ATAAAGCTCT GGAGCACAGC TCTTCTCCAG CCACTCCAGG TTTGGCCCGT 
CAGCAGCTCA TGATGTCCAC CAAGTCCAAC AAGTCTCCAG AGCACCAGTC CAGCGGCGGC 
CTGATGTCCT CAAACTCCAG CCGCGGACGC AAAGACAACG CCAATCCTGA AGAAATAAGG 
AGAGTCACTT CTGAAAAGTA CACCAGACGT GTGAAGGACC GGATTCATCA CAACACTCCT 
CACCGCTTCA CGCAGGGGCT CAATATGAGA GCGGCTCGCT GTACTGTGTG TCTGGACACA 
GTCCATTTTG GACGTCAGGC TGCAACATGC ATTGAATGTC ACGCGTTGTG TCACCCAAAA 
TGCTCCCCGT ACCTCCCTGC CACCTGCGGC GTTCCCACTG AATACGCGCT GCACCTCTCA 
GAGGCTCTGT GTCGGGAGAA GGGAAGCACT TCCGGCCTGA AAGATATGGG TGGACATATG 
AGGCTTGAAG GATGGCTGAA GCAGCCCAGG AACGGGAAGC GCGGGCAGGG CTGGGAGAGG 
AAGTATGTGG TGCTGGATGG ATATAAAGTG ATTACATTCG AGACAGAACC AAGAGACGAG 
TCAGTGAAGC CGCTGGATGA GTTTGAGCTG TGTCTCTCTG ATGGAGAAGT GACCGTTCAT 
TCTGCTGTGG GTTCCTCTGA GCTCCCCAAC ACCGCTAAAA CAGATGTGCC GTACGTGCTG 
AAGCTGGAGT CGTCTCCTCA GTCCACATGC TGGCCCGGTC AGACTCTGTA CCTCATGACC 
TCGGGATACT CAGAGAAGCA GCGCTGGGTG GCAGTGCTGG AAGCCATCGT GGCCAGCAGT 
CGCGGAGCCA GAGAGAAAGC TGAGGCAGAT GCTAAATTAT TGGGTAACTC TTTACTTAAG 
CTAGAAGGTG ACGACCGTCT GGATATCAAC TGCACCCTGC CGCTGACCGA CCAGATTGTG 
TTGGTGGGCT CTGAGGAGGG TCTCTACGCA CTGAATGTGA TTAAGAACTC CCTCACTCAT 
ATCCCGGGTC TGGGATCCGT GTTTCAGATC CACATTATCA AAGATCATGA CAAGCTGCTG 
ATGATCGTGG GAGACGAGCG AGCGCTGTGT CTGGTGGAGA TTAAGAAGGT GAAGCAGTCT 
TTATCCCAGT CTCACCTCCC CACACTGCCC GAGATTGTGC CGTATATCTT TGAGACCGTC 
AAGGGCTGTC ATCTGTTTGC CTCGGGCAGG ATAGAAAATG GCCCATGCAT TTGTGCTGCG 
ATGCCCAATA AAGTCACGAT TCTACGATAC AATGACAATC TTAACAAGTT CTGCATACGG 
AAGGAGATTG AAACTCTGGA GCCGTGCAGC TGCATCCACT TCACCAGCTA CAGCATCATC 
ATTGGTACCA ATAAATTCTA CGAGATCGAG ATGAAGCAGT GCGTTCTGGA CGAGTTCCTG 
GACAAGAATG ACGTGTCGCT GGCGTCGGCT GTCTTCACCG GATCCTCCCA CAGTTTCCCC 
ATCTCTATCG TCCAGGTGTC CAGCACTCCG CAGAAGGAGG AATATCTGCT GTGTTTCCAC 
GAGATCGGAG TTTTTGTGGA TGCTTACGGT CGAAGAAGTC GCCCTGATGA TCTGAAGTGG 
AGTCGACTTC CCCTGACGTT CGCCTTCAGA GAGCCGTATC TCTTTGTGAC CTACTTCAAC 
TCTCTGGATG TTATTGAGAT TCAGGGACAC GCAGCTCTTG GGCCTCCCAG TCTGGTTCAC 
CTGGACATTC CTAACCCACG TTATCTGGGT CCAGCCATAT CGTCTGGTGC CATCTATCTG 
GCCTCGTCCT ACCAGAACAA ACTGCGGGTG ATCTGCTGTA AGGGCAGTCT GGTGCGGGAG 
TCTGGAGAGC TGCAGAGGAC CGGCTCCAGC AGGAGCAGCC CCAACAAGCG CGGTCCACCA 
ACATACAACG AGCACATCTC CAAACGTCTG GCCTCGAGTC CTGGAGCCCA GGAGGGGCCC 
CTGCACCGCG AGCCCAGCAC TCCTCACCGC TACCGCGAGG GCCGCACCGA GTTCCGGAGA 
GACAAATCTC CAGCCCGGCC CCTGGAGCGA GAGAAATCCC CGGGCCGTCC GGTGCTAGAC 
AGCCGCAGAG AGAGGTCTCC CGGTCGCTTC GAGAGCAGCG GCAGCGAGAG AGACCGCGAG 
CGGACACGAC TGCACACTAG CTCTGTACGC ACCCAGCTGA CCCCCGTCAA CAAGGTTTGG 
GACCAGTCTT CGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100333880 ( XM_009303725.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302000.2 Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X10, mRNA.

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CloneID	ODa60012
Clone ID Related Accession (Same CDS sequence)	XM_009303725.2 , XM_009303725.3
Accession Version	XM_009303725.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	citron Rho-interacting kinase-like isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303725.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG AGATTTCCAA AGTGCAGCGT AAGATGTCAG ACTTGGAGTC AGTTTTGCAG 
CAGAAAGACG TGGAGCTCAA GGCTTCAGAA ACCCAGAGAA ACATCTTAGA GCAAGACCTG 
GCAACCTATA TCACCGAATG CAGCAGTTTG AAGCGCAGTT TGGAGCAGGC CCGCATGGAG 
GTTTCGCAGG AAGATGATAA AGCTCTTCAG CTGCTGCACG ACATTCGAGA GCAGAGCAAC 
AAACTGCAGG AAATCAAAGA ACAGGAGTAC CACGCTCAGC TGGAGGAGAT GCGAGTGTCT 
ATTCGTCAGC TGGAAGAGAA TCTGTCGGCC GCTCGTCGCC GCAGTGACCT GTACGAGTCT 
GAGCTCAAAG AGTCCCGTCA GACCAGTGAG GAGCTCAAGA GGAAGGCAGT GGAGTATCAG 
CAGAAGATCC AGAAGGCTAA AGAACAAGGG AAAGTTGAGA TGGAGGAGCT TCTGAGCAAA 
CTGCAGAAGA CCAATGGAGA GCAACAAGTA AAGATTGAAG AGCTCCAGGA GAAGCTTGCT 
AAGGCAGTGA AGGCGAGTAC TGAAGCCACT GAACTCCTCC AGAATGTCCG TCAGGCTAAA 
GAGCGAATGG AGCGAGACCT GGAGCGTCTG CAGACCAAAG AGGACTCGTC CGACAGCCTG 
CGCAGACGCT TACGAGAGAC TGAGGATGGC AAAAAATCCC TGGAGAACCA GGTGAAGAGG 
CTGGAGATGG TGGAGCGGAG AGAATCCAAA CTGAAAGACG ACATTCAGAC CAAATCGCAG 
CAGATCCAGC AGATGGCCGA CAAAATCATG GAGCTTGAAG AAAATTTGCG GGACTCGCAG 
GCGATGGCGC AGCGCATGGA AACACAGCTG GAGCAGAAGG AAAAGCTTTT TGAAGACAAG 
ATCAAGGTTT TAGAAACTCA AATGAAGGCT GATTTGGCTG AGAAGGATTT GCTGGAGTCC 
AAACAGAGCA CTTTTGAAGA GGAGTCTCGC GAACGGGGAA AAGTGATCAG CGACCAGGCA 
GCGACGATCA ATGCCATGGA GTCAAAGATG ACCAGTCTGG AGCAGAGGAT CGCTGAGCTG 
TCCGAAGCCA ACAAACTGGC AGCAAACAGC AGCATTTACA CACAGAAGAA CATGAAAGCC 
CAGGAGGAGA TGATTTCTGA ACTCAGACAG CAGAAGTTTT ATTTGGAGTC TCAGGCTGGA 
AAACTGGAGG CGCAGAATGC AAAGCTGGAG GAGCATCTGG AGAAACTCAC CCAACAAGAG 
CAGACAAACA AGAGCCGAGT GCTGGAGCTG GAAGCCAGGC TGCGAGAGAT CGGCCTAGAG 
CATGAGGAAC AGAAGCTGGA GATCAAGCGT CAGGTGTCGG AGCTCACGTT GTCTCTGCAG 
GAGAGAGAAG CTCAGATCAG CAGCCTGCAA GCGGCACGCC ACGCTCTCGA CAGCCAACTA 
CAGCAGGCCA AAACTGAGCT GGAGGAGACC ACCGCCGAGG CCGAAGAGGA GATCACCGTC 
CTGAGGGCCC ATCGGGATGA AATCCAACGC AAGTTTGATG CCCTGCGAGA CAGCTGTGCG 
GTGATCACGG ATCTGGAGGA GCAGTTGACG CAGCTCACGC AAGAAAACGC GGAGCTGAAC 
CGGCAGAACT TCTACCTGTC CAAACAGCTG GACGAGGCCA CAGACGAGAA CGAGGAGCGC 
CTGCAGCTCA AGCAGGACGT GGAGCGCCTC AGGAGAGAGG TGGCAGACCG AGAGATGCAC 
CTCAACAACC AGAAACAGAA CATCGAGACG CTGAAGACGA CATGTACAAT GCTGGAGGAG 
CAGGTTATGG AGCTGGAGAC TCTGAATGAT GAGCTGATGG AGAAGGAGAG GCAGTGGGAG 
AACTGGAGAT CTGCTCTGGA CGAGGAGAAA AACCAGGCCG AGCGCAGAGC CAGAGACCTG 
CAAAGACAGC TGGACAGCGA GAAGCAGAAC CGACTGCGTG CAGAACAGAG AAACTCTGAG 
TCTCGGCAGG CCGTGGAGCT GGCGGTGAAA GAGCACAAAG CTGAAATCCT GGTTCTGCAG 
CAGGCGCTCA AAGACCAGAA ACTCAAGGCT GAGGGCCTTT CTGACACTCT GGGTGATCTG 
GAGAAGAAGC ACGGCATGCT GGAGATGAAC GCTCGCAGCC TGCAGCAGAA GCTGGAGACG 
GAGAGAGAGA TGAAGCAGAG ACTCATGGAC GAGCAAGCTA AACTGCAGCA GCAGCTGGAC 
ATGCAGAAGA GCCACATCTT CAGACTGACT CAAGGACTGC AGGATGCGCT GGACCAGACC 
GACCTGCTGA AGACCGAGCG CACAGACCTG GAGTACCAGC TGGAAAACAT ACAGGCGGCG 
TATTCTCACG AGAAGGTGAA GATGGAGGGG ACGATCACCC AGCAGACCAA GCTCATAGAC 
TTCCTGCAGG CCAAGGTAGA GCACCCCTCT AAGAAGAAAA AGGGGCTGTT TGGTGGCCGG 
CGGCGGGAGG ATCTGCTAGC GGCGCTGGCG GCTCAGGGAC AGACACAGGT GCCTGTGGTG 
CCCGCCGCCA CTCCTGTGCC CCTGCAGTAC AGCGACCTGA AGGCGGCGCT GGATAAAGAG 
CGAGCCCGAT GCTCCGAGCT GGAGGACGCC CTGCAGAAAA CACGCCTGGA GCTCAGAGAA 
GTTCAGTTTA ATAAAGCTCT GGAGCACAGC TCTTCTCCAG CCACTCCAGG TTTGGCCCGT 
CAGCAGCTCA TGATGTCCAC CAAGTCCAAC AAGTCTCCAG AGCACCAGTC CAGCGGCGGC 
CTGATGTCCT CAAACTCCAG CCGCGGACGC AAAGACAACG CCAATCCTGA AGAGTACACC 
AGACGTGTGA AGGACCGGAT TCATCACAAC ACTCCTCACC GCTTCACGCA GGGGCTCAAT 
ATGAGAGCGG CTCGCTGTAC TGTGTGTCTG GACACAGTCC ATTTTGGACG TCAGGCTGCA 
ACATGCATTG AATGTCACGC GTTGTGTCAC CCAAAATGCT CCCCGTACCT CCCTGCCACC 
TGCGGCGTTC CCACTGAATA CGCGCTGCAC CTCTCAGAGG CTCTGTGTCG GGAGAAGGGA 
AGCACTTCCG GCCTGAAAGA TATGGGTGGA CATATGAGGC TTGAAGGATG GCTGAAGCAG 
CCCAGGAACG GGAAGCGCGG GCAGGGCTGG GAGAGGAAGT ATGTGGTGCT GGATGGATAT 
AAAGTGATTA CATTCGAGAC AGAACCAAGA GACGAGTCAG TGAAGCCGCT GGATGAGTTT 
GAGCTGTGTC TCTCTGATGG AGAAGTGACC GTTCATTCTG CTGTGGGTTC CTCTGAGCTC 
CCCAACACCG CTAAAACAGA TGTGCCGTAC GTGCTGAAGC TGGAGTCGTC TCCTCAGTCC 
ACATGCTGGC CCGGTCAGAC TCTGTACCTC ATGACCTCGG GATACTCAGA GAAGCAGCGC 
TGGGTGGCAG TGCTGGAAGC CATCGTGGCC AGCAGTCGCG GAGCCAGAGA GAAAGCTGAG 
GCAGATGCTA AATTATTGGG TAACTCTTTA CTTAAGCTAG AAGGTGACGA CCGTCTGGAT 
ATCAACTGCA CCCTGCCGCT GACCGACCAG ATTGTGTTGG TGGGCTCTGA GGAGGGTCTC 
TACGCACTGA ATGTGATTAA GAACTCCCTC ACTCATATCC CGGGTCTGGG ATCCGTGTTT 
CAGATCCACA TTATCAAAGA TCATGACAAG CTGCTGATGA TCGTGGGAGA CGAGCGAGCG 
CTGTGTCTGG TGGAGATTAA GAAGGTGAAG CAGTCTTTAT CCCAGTCTCA CCTCCCCACA 
CTGCCCGAGA TTGTGCCGTA TATCTTTGAG ACCGTCAAGG GCTGTCATCT GTTTGCCTCG 
GGCAGGATAG AAAATGGCCC ATGCATTTGT GCTGCGATGC CCAATAAAGT CACGATTCTA 
CGATACAATG ACAATCTTAA CAAGTTCTGC ATACGGAAGG AGATTGAAAC TCTGGAGCCG 
TGCAGCTGCA TCCACTTCAC CAGCTACAGC ATCATCATTG GTACCAATAA ATTCTACGAG 
ATCGAGATGA AGCAGTGCGT TCTGGACGAG TTCCTGGACA AGAATGACGT GTCGCTGGCG 
TCGGCTGTCT TCACCGGATC CTCCCACAGT TTCCCCATCT CTATCGTCCA GGTGTCCAGC 
ACTCCGCAGA AGGAGGAATA TCTGCTGTGT TTCCACGAGA TCGGAGTTTT TGTGGATGCT 
TACGGTCGAA GAAGTCGCCC TGATGATCTG AAGTGGAGTC GACTTCCCCT GACGTTCGCC 
TTCAGAGAGC CGTATCTCTT TGTGACCTAC TTCAACTCTC TGGATGTTAT TGAGATTCAG 
GGACACGCAG CTCTTGGGCC TCCCAGTCTG GTTCACCTGG ACATTCCTAA CCCACGTTAT 
CTGGGTCCAG CCATATCGTC TGGTGCCATC TATCTGGCCT CGTCCTACCA GAACAAACTG 
CGGGTGATCT GCTGTAAGGG CAGTCTGGTG CGGGAGTCTG GAGAGCTGCA GAGGACCGGC 
TCCAGCAGGA GCAGCCCCAA CAAGCGCGGT CCACCAACAT ACAACGAGCA CATCTCCAAA 
CGTCTGGCCT CGAGTCCTGG AGCCCAGGAG GGGCCCCTGC ACCGCGAGCC CAGCACTCCT 
CACCGCTACC GCGAGGGCCG CACCGAGTTC CGGAGAGACA AATCTCCAGC CCGGCCCCTG 
GAGCGAGAGA AATCCCCGGG CCGTCCGGTG CTAGACAGCC GCAGAGAGAG GTCTCCCGGT 
CGCTTCGAGA GCAGCGGCAG CGAGAGAGAC CGCGAGCGGA CACGACTGCA CACTAGCTCT 
GTACGCACCC AGCTGACCCC CGTCAACAAG GTTTGGGACC AGTCTTCGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302000.2
CDS	434..5287
Translation	MEQEISKVQRKMSDLESVLQQKDVELKASETQRNILEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSNKLQEIKEQEYHAQLEEMRVSIRQLEENLSAARRRSDLYESELKESRQTSEELKRKAVEYQQKIQKAKEQGKVEMEELLSKLQKTNGEQQVKIEELQEKLAKAVKASTEATELLQNVRQAKERMERDLERLQTKEDSSDSLRRRLRETEDGKKSLENQVKRLEMVERRESKLKDDIQTKSQQIQQMADKIMELEENLRDSQAMAQRMETQLEQKEKLFEDKIKVLETQMKADLAEKDLLESKQSTFEEESRERGKVISDQAATINAMESKMTSLEQRIAELSEANKLAANSSIYTQKNMKAQEEMISELRQQKFYLESQAGKLEAQNAKLEEHLEKLTQQEQTNKSRVLELEARLREIGLEHEEQKLEIKRQVSELTLSLQEREAQISSLQAARHALDSQLQQAKTELEETTAEAEEEITVLRAHRDEIQRKFDALRDSCAVITDLEEQLTQLTQENAELNRQNFYLSKQLDEATDENEERLQLKQDVERLRREVADREMHLNNQKQNIETLKTTCTMLEEQVMELETLNDELMEKERQWENWRSALDEEKNQAERRARDLQRQLDSEKQNRLRAEQRNSESRQAVELAVKEHKAEILVLQQALKDQKLKAEGLSDTLGDLEKKHGMLEMNARSLQQKLETEREMKQRLMDEQAKLQQQLDMQKSHIFRLTQGLQDALDQTDLLKTERTDLEYQLENIQAAYSHEKVKMEGTITQQTKLIDFLQAKVEHPSKKKKGLFGGRRREDLLAALAAQGQTQVPVVPAATPVPLQYSDLKAALDKERARCSELEDALQKTRLELREVQFNKALEHSSSPATPGLARQQLMMSTKSNKSPEHQSSGGLMSSNSSRGRKDNANPEEYTRRVKDRIHHNTPHRFTQGLNMRAARCTVCLDTVHFGRQAATCIECHALCHPKCSPYLPATCGVPTEYALHLSEALCREKGSTSGLKDMGGHMRLEGWLKQPRNGKRGQGWERKYVVLDGYKVITFETEPRDESVKPLDEFELCLSDGEVTVHSAVGSSELPNTAKTDVPYVLKLESSPQSTCWPGQTLYLMTSGYSEKQRWVAVLEAIVASSRGAREKAEADAKLLGNSLLKLEGDDRLDINCTLPLTDQIVLVGSEEGLYALNVIKNSLTHIPGLGSVFQIHIIKDHDKLLMIVGDERALCLVEIKKVKQSLSQSHLPTLPEIVPYIFETVKGCHLFASGRIENGPCICAAMPNKVTILRYNDNLNKFCIRKEIETLEPCSCIHFTSYSIIIGTNKFYEIEMKQCVLDEFLDKNDVSLASAVFTGSSHSFPISIVQVSSTPQKEEYLLCFHEIGVFVDAYGRRSRPDDLKWSRLPLTFAFREPYLFVTYFNSLDVIEIQGHAALGPPSLVHLDIPNPRYLGPAISSGAIYLASSYQNKLRVICCKGSLVRESGELQRTGSSRSSPNKRGPPTYNEHISKRLASSPGAQEGPLHREPSTPHRYREGRTEFRRDKSPARPLEREKSPGRPVLDSRRERSPGRFESSGSERDRERTRLHTSSVRTQLTPVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_009303725.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303725.2

ORF Insert Sequence:

ATGGAGCAGG AGATTTCCAA AGTGCAGCGT AAGATGTCAG ACTTGGAGTC AGTTTTGCAG 
CAGAAAGACG TGGAGCTCAA GGCTTCAGAA ACCCAGAGAA ACATCTTAGA GCAAGACCTG 
GCAACCTATA TCACCGAATG CAGCAGTTTG AAGCGCAGTT TGGAGCAGGC CCGCATGGAG 
GTTTCGCAGG AAGATGATAA AGCTCTTCAG CTGCTGCACG ACATTCGAGA GCAGAGCAAC 
AAACTGCAGG AAATCAAAGA ACAGGAGTAC CACGCTCAGC TGGAGGAGAT GCGAGTGTCT 
ATTCGTCAGC TGGAAGAGAA TCTGTCGGCC GCTCGTCGCC GCAGTGACCT GTACGAGTCT 
GAGCTCAAAG AGTCCCGTCA GACCAGTGAG GAGCTCAAGA GGAAGGCAGT GGAGTATCAG 
CAGAAGATCC AGAAGGCTAA AGAACAAGGG AAAGTTGAGA TGGAGGAGCT TCTGAGCAAA 
CTGCAGAAGA CCAATGGAGA GCAACAAGTA AAGATTGAAG AGCTCCAGGA GAAGCTTGCT 
AAGGCAGTGA AGGCGAGTAC TGAAGCCACT GAACTCCTCC AGAATGTCCG TCAGGCTAAA 
GAGCGAATGG AGCGAGACCT GGAGCGTCTG CAGACCAAAG AGGACTCGTC CGACAGCCTG 
CGCAGACGCT TACGAGAGAC TGAGGATGGC AAAAAATCCC TGGAGAACCA GGTGAAGAGG 
CTGGAGATGG TGGAGCGGAG AGAATCCAAA CTGAAAGACG ACATTCAGAC CAAATCGCAG 
CAGATCCAGC AGATGGCCGA CAAAATCATG GAGCTTGAAG AAAATTTGCG GGACTCGCAG 
GCGATGGCGC AGCGCATGGA AACACAGCTG GAGCAGAAGG AAAAGCTTTT TGAAGACAAG 
ATCAAGGTTT TAGAAACTCA AATGAAGGCT GATTTGGCTG AGAAGGATTT GCTGGAGTCC 
AAACAGAGCA CTTTTGAAGA GGAGTCTCGC GAACGGGGAA AAGTGATCAG CGACCAGGCA 
GCGACGATCA ATGCCATGGA GTCAAAGATG ACCAGTCTGG AGCAGAGGAT CGCTGAGCTG 
TCCGAAGCCA ACAAACTGGC AGCAAACAGC AGCATTTACA CACAGAAGAA CATGAAAGCC 
CAGGAGGAGA TGATTTCTGA ACTCAGACAG CAGAAGTTTT ATTTGGAGTC TCAGGCTGGA 
AAACTGGAGG CGCAGAATGC AAAGCTGGAG GAGCATCTGG AGAAACTCAC CCAACAAGAG 
CAGACAAACA AGAGCCGAGT GCTGGAGCTG GAAGCCAGGC TGCGAGAGAT CGGCCTAGAG 
CATGAGGAAC AGAAGCTGGA GATCAAGCGT CAGGTGTCGG AGCTCACGTT GTCTCTGCAG 
GAGAGAGAAG CTCAGATCAG CAGCCTGCAA GCGGCACGCC ACGCTCTCGA CAGCCAACTA 
CAGCAGGCCA AAACTGAGCT GGAGGAGACC ACCGCCGAGG CCGAAGAGGA GATCACCGTC 
CTGAGGGCCC ATCGGGATGA AATCCAACGC AAGTTTGATG CCCTGCGAGA CAGCTGTGCG 
GTGATCACGG ATCTGGAGGA GCAGTTGACG CAGCTCACGC AAGAAAACGC GGAGCTGAAC 
CGGCAGAACT TCTACCTGTC CAAACAGCTG GACGAGGCCA CAGACGAGAA CGAGGAGCGC 
CTGCAGCTCA AGCAGGACGT GGAGCGCCTC AGGAGAGAGG TGGCAGACCG AGAGATGCAC 
CTCAACAACC AGAAACAGAA CATCGAGACG CTGAAGACGA CATGTACAAT GCTGGAGGAG 
CAGGTTATGG AGCTGGAGAC TCTGAATGAT GAGCTGATGG AGAAGGAGAG GCAGTGGGAG 
AACTGGAGAT CTGCTCTGGA CGAGGAGAAA AACCAGGCCG AGCGCAGAGC CAGAGACCTG 
CAAAGACAGC TGGACAGCGA GAAGCAGAAC CGACTGCGTG CAGAACAGAG AAACTCTGAG 
TCTCGGCAGG CCGTGGAGCT GGCGGTGAAA GAGCACAAAG CTGAAATCCT GGTTCTGCAG 
CAGGCGCTCA AAGACCAGAA ACTCAAGGCT GAGGGCCTTT CTGACACTCT GGGTGATCTG 
GAGAAGAAGC ACGGCATGCT GGAGATGAAC GCTCGCAGCC TGCAGCAGAA GCTGGAGACG 
GAGAGAGAGA TGAAGCAGAG ACTCATGGAC GAGCAAGCTA AACTGCAGCA GCAGCTGGAC 
ATGCAGAAGA GCCACATCTT CAGACTGACT CAAGGACTGC AGGATGCGCT GGACCAGACC 
GACCTGCTGA AGACCGAGCG CACAGACCTG GAGTACCAGC TGGAAAACAT ACAGGCGGCG 
TATTCTCACG AGAAGGTGAA GATGGAGGGG ACGATCACCC AGCAGACCAA GCTCATAGAC 
TTCCTGCAGG CCAAGGTAGA GCACCCCTCT AAGAAGAAAA AGGGGCTGTT TGGTGGCCGG 
CGGCGGGAGG ATCTGCTAGC GGCGCTGGCG GCTCAGGGAC AGACACAGGT GCCTGTGGTG 
CCCGCCGCCA CTCCTGTGCC CCTGCAGTAC AGCGACCTGA AGGCGGCGCT GGATAAAGAG 
CGAGCCCGAT GCTCCGAGCT GGAGGACGCC CTGCAGAAAA CACGCCTGGA GCTCAGAGAA 
GTTCAGTTTA ATAAAGCTCT GGAGCACAGC TCTTCTCCAG CCACTCCAGG TTTGGCCCGT 
CAGCAGCTCA TGATGTCCAC CAAGTCCAAC AAGTCTCCAG AGCACCAGTC CAGCGGCGGC 
CTGATGTCCT CAAACTCCAG CCGCGGACGC AAAGACAACG CCAATCCTGA AGAGTACACC 
AGACGTGTGA AGGACCGGAT TCATCACAAC ACTCCTCACC GCTTCACGCA GGGGCTCAAT 
ATGAGAGCGG CTCGCTGTAC TGTGTGTCTG GACACAGTCC ATTTTGGACG TCAGGCTGCA 
ACATGCATTG AATGTCACGC GTTGTGTCAC CCAAAATGCT CCCCGTACCT CCCTGCCACC 
TGCGGCGTTC CCACTGAATA CGCGCTGCAC CTCTCAGAGG CTCTGTGTCG GGAGAAGGGA 
AGCACTTCCG GCCTGAAAGA TATGGGTGGA CATATGAGGC TTGAAGGATG GCTGAAGCAG 
CCCAGGAACG GGAAGCGCGG GCAGGGCTGG GAGAGGAAGT ATGTGGTGCT GGATGGATAT 
AAAGTGATTA CATTCGAGAC AGAACCAAGA GACGAGTCAG TGAAGCCGCT GGATGAGTTT 
GAGCTGTGTC TCTCTGATGG AGAAGTGACC GTTCATTCTG CTGTGGGTTC CTCTGAGCTC 
CCCAACACCG CTAAAACAGA TGTGCCGTAC GTGCTGAAGC TGGAGTCGTC TCCTCAGTCC 
ACATGCTGGC CCGGTCAGAC TCTGTACCTC ATGACCTCGG GATACTCAGA GAAGCAGCGC 
TGGGTGGCAG TGCTGGAAGC CATCGTGGCC AGCAGTCGCG GAGCCAGAGA GAAAGCTGAG 
GCAGATGCTA AATTATTGGG TAACTCTTTA CTTAAGCTAG AAGGTGACGA CCGTCTGGAT 
ATCAACTGCA CCCTGCCGCT GACCGACCAG ATTGTGTTGG TGGGCTCTGA GGAGGGTCTC 
TACGCACTGA ATGTGATTAA GAACTCCCTC ACTCATATCC CGGGTCTGGG ATCCGTGTTT 
CAGATCCACA TTATCAAAGA TCATGACAAG CTGCTGATGA TCGTGGGAGA CGAGCGAGCG 
CTGTGTCTGG TGGAGATTAA GAAGGTGAAG CAGTCTTTAT CCCAGTCTCA CCTCCCCACA 
CTGCCCGAGA TTGTGCCGTA TATCTTTGAG ACCGTCAAGG GCTGTCATCT GTTTGCCTCG 
GGCAGGATAG AAAATGGCCC ATGCATTTGT GCTGCGATGC CCAATAAAGT CACGATTCTA 
CGATACAATG ACAATCTTAA CAAGTTCTGC ATACGGAAGG AGATTGAAAC TCTGGAGCCG 
TGCAGCTGCA TCCACTTCAC CAGCTACAGC ATCATCATTG GTACCAATAA ATTCTACGAG 
ATCGAGATGA AGCAGTGCGT TCTGGACGAG TTCCTGGACA AGAATGACGT GTCGCTGGCG 
TCGGCTGTCT TCACCGGATC CTCCCACAGT TTCCCCATCT CTATCGTCCA GGTGTCCAGC 
ACTCCGCAGA AGGAGGAATA TCTGCTGTGT TTCCACGAGA TCGGAGTTTT TGTGGATGCT 
TACGGTCGAA GAAGTCGCCC TGATGATCTG AAGTGGAGTC GACTTCCCCT GACGTTCGCC 
TTCAGAGAGC CGTATCTCTT TGTGACCTAC TTCAACTCTC TGGATGTTAT TGAGATTCAG 
GGACACGCAG CTCTTGGGCC TCCCAGTCTG GTTCACCTGG ACATTCCTAA CCCACGTTAT 
CTGGGTCCAG CCATATCGTC TGGTGCCATC TATCTGGCCT CGTCCTACCA GAACAAACTG 
CGGGTGATCT GCTGTAAGGG CAGTCTGGTG CGGGAGTCTG GAGAGCTGCA GAGGACCGGC 
TCCAGCAGGA GCAGCCCCAA CAAGCGCGGT CCACCAACAT ACAACGAGCA CATCTCCAAA 
CGTCTGGCCT CGAGTCCTGG AGCCCAGGAG GGGCCCCTGC ACCGCGAGCC CAGCACTCCT 
CACCGCTACC GCGAGGGCCG CACCGAGTTC CGGAGAGACA AATCTCCAGC CCGGCCCCTG 
GAGCGAGAGA AATCCCCGGG CCGTCCGGTG CTAGACAGCC GCAGAGAGAG GTCTCCCGGT 
CGCTTCGAGA GCAGCGGCAG CGAGAGAGAC CGCGAGCGGA CACGACTGCA CACTAGCTCT 
GTACGCACCC AGCTGACCCC CGTCAACAAG GTTTGGGACC AGTCTTCGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100333880 ( XM_009303725.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302000.2 Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X12, mRNA.

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CloneID	ODa60012
Clone ID Related Accession (Same CDS sequence)	XM_009303725.2 , XM_009303725.3
Accession Version	XM_009303725.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	citron Rho-interacting kinase-like isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303725.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG AGATTTCCAA AGTGCAGCGT AAGATGTCAG ACTTGGAGTC AGTTTTGCAG 
CAGAAAGACG TGGAGCTCAA GGCTTCAGAA ACCCAGAGAA ACATCTTAGA GCAAGACCTG 
GCAACCTATA TCACCGAATG CAGCAGTTTG AAGCGCAGTT TGGAGCAGGC CCGCATGGAG 
GTTTCGCAGG AAGATGATAA AGCTCTTCAG CTGCTGCACG ACATTCGAGA GCAGAGCAAC 
AAACTGCAGG AAATCAAAGA ACAGGAGTAC CACGCTCAGC TGGAGGAGAT GCGAGTGTCT 
ATTCGTCAGC TGGAAGAGAA TCTGTCGGCC GCTCGTCGCC GCAGTGACCT GTACGAGTCT 
GAGCTCAAAG AGTCCCGTCA GACCAGTGAG GAGCTCAAGA GGAAGGCAGT GGAGTATCAG 
CAGAAGATCC AGAAGGCTAA AGAACAAGGG AAAGTTGAGA TGGAGGAGCT TCTGAGCAAA 
CTGCAGAAGA CCAATGGAGA GCAACAAGTA AAGATTGAAG AGCTCCAGGA GAAGCTTGCT 
AAGGCAGTGA AGGCGAGTAC TGAAGCCACT GAACTCCTCC AGAATGTCCG TCAGGCTAAA 
GAGCGAATGG AGCGAGACCT GGAGCGTCTG CAGACCAAAG AGGACTCGTC CGACAGCCTG 
CGCAGACGCT TACGAGAGAC TGAGGATGGC AAAAAATCCC TGGAGAACCA GGTGAAGAGG 
CTGGAGATGG TGGAGCGGAG AGAATCCAAA CTGAAAGACG ACATTCAGAC CAAATCGCAG 
CAGATCCAGC AGATGGCCGA CAAAATCATG GAGCTTGAAG AAAATTTGCG GGACTCGCAG 
GCGATGGCGC AGCGCATGGA AACACAGCTG GAGCAGAAGG AAAAGCTTTT TGAAGACAAG 
ATCAAGGTTT TAGAAACTCA AATGAAGGCT GATTTGGCTG AGAAGGATTT GCTGGAGTCC 
AAACAGAGCA CTTTTGAAGA GGAGTCTCGC GAACGGGGAA AAGTGATCAG CGACCAGGCA 
GCGACGATCA ATGCCATGGA GTCAAAGATG ACCAGTCTGG AGCAGAGGAT CGCTGAGCTG 
TCCGAAGCCA ACAAACTGGC AGCAAACAGC AGCATTTACA CACAGAAGAA CATGAAAGCC 
CAGGAGGAGA TGATTTCTGA ACTCAGACAG CAGAAGTTTT ATTTGGAGTC TCAGGCTGGA 
AAACTGGAGG CGCAGAATGC AAAGCTGGAG GAGCATCTGG AGAAACTCAC CCAACAAGAG 
CAGACAAACA AGAGCCGAGT GCTGGAGCTG GAAGCCAGGC TGCGAGAGAT CGGCCTAGAG 
CATGAGGAAC AGAAGCTGGA GATCAAGCGT CAGGTGTCGG AGCTCACGTT GTCTCTGCAG 
GAGAGAGAAG CTCAGATCAG CAGCCTGCAA GCGGCACGCC ACGCTCTCGA CAGCCAACTA 
CAGCAGGCCA AAACTGAGCT GGAGGAGACC ACCGCCGAGG CCGAAGAGGA GATCACCGTC 
CTGAGGGCCC ATCGGGATGA AATCCAACGC AAGTTTGATG CCCTGCGAGA CAGCTGTGCG 
GTGATCACGG ATCTGGAGGA GCAGTTGACG CAGCTCACGC AAGAAAACGC GGAGCTGAAC 
CGGCAGAACT TCTACCTGTC CAAACAGCTG GACGAGGCCA CAGACGAGAA CGAGGAGCGC 
CTGCAGCTCA AGCAGGACGT GGAGCGCCTC AGGAGAGAGG TGGCAGACCG AGAGATGCAC 
CTCAACAACC AGAAACAGAA CATCGAGACG CTGAAGACGA CATGTACAAT GCTGGAGGAG 
CAGGTTATGG AGCTGGAGAC TCTGAATGAT GAGCTGATGG AGAAGGAGAG GCAGTGGGAG 
AACTGGAGAT CTGCTCTGGA CGAGGAGAAA AACCAGGCCG AGCGCAGAGC CAGAGACCTG 
CAAAGACAGC TGGACAGCGA GAAGCAGAAC CGACTGCGTG CAGAACAGAG AAACTCTGAG 
TCTCGGCAGG CCGTGGAGCT GGCGGTGAAA GAGCACAAAG CTGAAATCCT GGTTCTGCAG 
CAGGCGCTCA AAGACCAGAA ACTCAAGGCT GAGGGCCTTT CTGACACTCT GGGTGATCTG 
GAGAAGAAGC ACGGCATGCT GGAGATGAAC GCTCGCAGCC TGCAGCAGAA GCTGGAGACG 
GAGAGAGAGA TGAAGCAGAG ACTCATGGAC GAGCAAGCTA AACTGCAGCA GCAGCTGGAC 
ATGCAGAAGA GCCACATCTT CAGACTGACT CAAGGACTGC AGGATGCGCT GGACCAGACC 
GACCTGCTGA AGACCGAGCG CACAGACCTG GAGTACCAGC TGGAAAACAT ACAGGCGGCG 
TATTCTCACG AGAAGGTGAA GATGGAGGGG ACGATCACCC AGCAGACCAA GCTCATAGAC 
TTCCTGCAGG CCAAGGTAGA GCACCCCTCT AAGAAGAAAA AGGGGCTGTT TGGTGGCCGG 
CGGCGGGAGG ATCTGCTAGC GGCGCTGGCG GCTCAGGGAC AGACACAGGT GCCTGTGGTG 
CCCGCCGCCA CTCCTGTGCC CCTGCAGTAC AGCGACCTGA AGGCGGCGCT GGATAAAGAG 
CGAGCCCGAT GCTCCGAGCT GGAGGACGCC CTGCAGAAAA CACGCCTGGA GCTCAGAGAA 
GTTCAGTTTA ATAAAGCTCT GGAGCACAGC TCTTCTCCAG CCACTCCAGG TTTGGCCCGT 
CAGCAGCTCA TGATGTCCAC CAAGTCCAAC AAGTCTCCAG AGCACCAGTC CAGCGGCGGC 
CTGATGTCCT CAAACTCCAG CCGCGGACGC AAAGACAACG CCAATCCTGA AGAGTACACC 
AGACGTGTGA AGGACCGGAT TCATCACAAC ACTCCTCACC GCTTCACGCA GGGGCTCAAT 
ATGAGAGCGG CTCGCTGTAC TGTGTGTCTG GACACAGTCC ATTTTGGACG TCAGGCTGCA 
ACATGCATTG AATGTCACGC GTTGTGTCAC CCAAAATGCT CCCCGTACCT CCCTGCCACC 
TGCGGCGTTC CCACTGAATA CGCGCTGCAC CTCTCAGAGG CTCTGTGTCG GGAGAAGGGA 
AGCACTTCCG GCCTGAAAGA TATGGGTGGA CATATGAGGC TTGAAGGATG GCTGAAGCAG 
CCCAGGAACG GGAAGCGCGG GCAGGGCTGG GAGAGGAAGT ATGTGGTGCT GGATGGATAT 
AAAGTGATTA CATTCGAGAC AGAACCAAGA GACGAGTCAG TGAAGCCGCT GGATGAGTTT 
GAGCTGTGTC TCTCTGATGG AGAAGTGACC GTTCATTCTG CTGTGGGTTC CTCTGAGCTC 
CCCAACACCG CTAAAACAGA TGTGCCGTAC GTGCTGAAGC TGGAGTCGTC TCCTCAGTCC 
ACATGCTGGC CCGGTCAGAC TCTGTACCTC ATGACCTCGG GATACTCAGA GAAGCAGCGC 
TGGGTGGCAG TGCTGGAAGC CATCGTGGCC AGCAGTCGCG GAGCCAGAGA GAAAGCTGAG 
GCAGATGCTA AATTATTGGG TAACTCTTTA CTTAAGCTAG AAGGTGACGA CCGTCTGGAT 
ATCAACTGCA CCCTGCCGCT GACCGACCAG ATTGTGTTGG TGGGCTCTGA GGAGGGTCTC 
TACGCACTGA ATGTGATTAA GAACTCCCTC ACTCATATCC CGGGTCTGGG ATCCGTGTTT 
CAGATCCACA TTATCAAAGA TCATGACAAG CTGCTGATGA TCGTGGGAGA CGAGCGAGCG 
CTGTGTCTGG TGGAGATTAA GAAGGTGAAG CAGTCTTTAT CCCAGTCTCA CCTCCCCACA 
CTGCCCGAGA TTGTGCCGTA TATCTTTGAG ACCGTCAAGG GCTGTCATCT GTTTGCCTCG 
GGCAGGATAG AAAATGGCCC ATGCATTTGT GCTGCGATGC CCAATAAAGT CACGATTCTA 
CGATACAATG ACAATCTTAA CAAGTTCTGC ATACGGAAGG AGATTGAAAC TCTGGAGCCG 
TGCAGCTGCA TCCACTTCAC CAGCTACAGC ATCATCATTG GTACCAATAA ATTCTACGAG 
ATCGAGATGA AGCAGTGCGT TCTGGACGAG TTCCTGGACA AGAATGACGT GTCGCTGGCG 
TCGGCTGTCT TCACCGGATC CTCCCACAGT TTCCCCATCT CTATCGTCCA GGTGTCCAGC 
ACTCCGCAGA AGGAGGAATA TCTGCTGTGT TTCCACGAGA TCGGAGTTTT TGTGGATGCT 
TACGGTCGAA GAAGTCGCCC TGATGATCTG AAGTGGAGTC GACTTCCCCT GACGTTCGCC 
TTCAGAGAGC CGTATCTCTT TGTGACCTAC TTCAACTCTC TGGATGTTAT TGAGATTCAG 
GGACACGCAG CTCTTGGGCC TCCCAGTCTG GTTCACCTGG ACATTCCTAA CCCACGTTAT 
CTGGGTCCAG CCATATCGTC TGGTGCCATC TATCTGGCCT CGTCCTACCA GAACAAACTG 
CGGGTGATCT GCTGTAAGGG CAGTCTGGTG CGGGAGTCTG GAGAGCTGCA GAGGACCGGC 
TCCAGCAGGA GCAGCCCCAA CAAGCGCGGT CCACCAACAT ACAACGAGCA CATCTCCAAA 
CGTCTGGCCT CGAGTCCTGG AGCCCAGGAG GGGCCCCTGC ACCGCGAGCC CAGCACTCCT 
CACCGCTACC GCGAGGGCCG CACCGAGTTC CGGAGAGACA AATCTCCAGC CCGGCCCCTG 
GAGCGAGAGA AATCCCCGGG CCGTCCGGTG CTAGACAGCC GCAGAGAGAG GTCTCCCGGT 
CGCTTCGAGA GCAGCGGCAG CGAGAGAGAC CGCGAGCGGA CACGACTGCA CACTAGCTCT 
GTACGCACCC AGCTGACCCC CGTCAACAAG GTTTGGGACC AGTCTTCGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302000.2
CDS	433..5286
Translation	MEQEISKVQRKMSDLESVLQQKDVELKASETQRNILEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSNKLQEIKEQEYHAQLEEMRVSIRQLEENLSAARRRSDLYESELKESRQTSEELKRKAVEYQQKIQKAKEQGKVEMEELLSKLQKTNGEQQVKIEELQEKLAKAVKASTEATELLQNVRQAKERMERDLERLQTKEDSSDSLRRRLRETEDGKKSLENQVKRLEMVERRESKLKDDIQTKSQQIQQMADKIMELEENLRDSQAMAQRMETQLEQKEKLFEDKIKVLETQMKADLAEKDLLESKQSTFEEESRERGKVISDQAATINAMESKMTSLEQRIAELSEANKLAANSSIYTQKNMKAQEEMISELRQQKFYLESQAGKLEAQNAKLEEHLEKLTQQEQTNKSRVLELEARLREIGLEHEEQKLEIKRQVSELTLSLQEREAQISSLQAARHALDSQLQQAKTELEETTAEAEEEITVLRAHRDEIQRKFDALRDSCAVITDLEEQLTQLTQENAELNRQNFYLSKQLDEATDENEERLQLKQDVERLRREVADREMHLNNQKQNIETLKTTCTMLEEQVMELETLNDELMEKERQWENWRSALDEEKNQAERRARDLQRQLDSEKQNRLRAEQRNSESRQAVELAVKEHKAEILVLQQALKDQKLKAEGLSDTLGDLEKKHGMLEMNARSLQQKLETEREMKQRLMDEQAKLQQQLDMQKSHIFRLTQGLQDALDQTDLLKTERTDLEYQLENIQAAYSHEKVKMEGTITQQTKLIDFLQAKVEHPSKKKKGLFGGRRREDLLAALAAQGQTQVPVVPAATPVPLQYSDLKAALDKERARCSELEDALQKTRLELREVQFNKALEHSSSPATPGLARQQLMMSTKSNKSPEHQSSGGLMSSNSSRGRKDNANPEEYTRRVKDRIHHNTPHRFTQGLNMRAARCTVCLDTVHFGRQAATCIECHALCHPKCSPYLPATCGVPTEYALHLSEALCREKGSTSGLKDMGGHMRLEGWLKQPRNGKRGQGWERKYVVLDGYKVITFETEPRDESVKPLDEFELCLSDGEVTVHSAVGSSELPNTAKTDVPYVLKLESSPQSTCWPGQTLYLMTSGYSEKQRWVAVLEAIVASSRGAREKAEADAKLLGNSLLKLEGDDRLDINCTLPLTDQIVLVGSEEGLYALNVIKNSLTHIPGLGSVFQIHIIKDHDKLLMIVGDERALCLVEIKKVKQSLSQSHLPTLPEIVPYIFETVKGCHLFASGRIENGPCICAAMPNKVTILRYNDNLNKFCIRKEIETLEPCSCIHFTSYSIIIGTNKFYEIEMKQCVLDEFLDKNDVSLASAVFTGSSHSFPISIVQVSSTPQKEEYLLCFHEIGVFVDAYGRRSRPDDLKWSRLPLTFAFREPYLFVTYFNSLDVIEIQGHAALGPPSLVHLDIPNPRYLGPAISSGAIYLASSYQNKLRVICCKGSLVRESGELQRTGSSRSSPNKRGPPTYNEHISKRLASSPGAQEGPLHREPSTPHRYREGRTEFRRDKSPARPLEREKSPGRPVLDSRRERSPGRFESSGSERDRERTRLHTSSVRTQLTPVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_009303725.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303725.3

ORF Insert Sequence:

ATGGAGCAGG AGATTTCCAA AGTGCAGCGT AAGATGTCAG ACTTGGAGTC AGTTTTGCAG 
CAGAAAGACG TGGAGCTCAA GGCTTCAGAA ACCCAGAGAA ACATCTTAGA GCAAGACCTG 
GCAACCTATA TCACCGAATG CAGCAGTTTG AAGCGCAGTT TGGAGCAGGC CCGCATGGAG 
GTTTCGCAGG AAGATGATAA AGCTCTTCAG CTGCTGCACG ACATTCGAGA GCAGAGCAAC 
AAACTGCAGG AAATCAAAGA ACAGGAGTAC CACGCTCAGC TGGAGGAGAT GCGAGTGTCT 
ATTCGTCAGC TGGAAGAGAA TCTGTCGGCC GCTCGTCGCC GCAGTGACCT GTACGAGTCT 
GAGCTCAAAG AGTCCCGTCA GACCAGTGAG GAGCTCAAGA GGAAGGCAGT GGAGTATCAG 
CAGAAGATCC AGAAGGCTAA AGAACAAGGG AAAGTTGAGA TGGAGGAGCT TCTGAGCAAA 
CTGCAGAAGA CCAATGGAGA GCAACAAGTA AAGATTGAAG AGCTCCAGGA GAAGCTTGCT 
AAGGCAGTGA AGGCGAGTAC TGAAGCCACT GAACTCCTCC AGAATGTCCG TCAGGCTAAA 
GAGCGAATGG AGCGAGACCT GGAGCGTCTG CAGACCAAAG AGGACTCGTC CGACAGCCTG 
CGCAGACGCT TACGAGAGAC TGAGGATGGC AAAAAATCCC TGGAGAACCA GGTGAAGAGG 
CTGGAGATGG TGGAGCGGAG AGAATCCAAA CTGAAAGACG ACATTCAGAC CAAATCGCAG 
CAGATCCAGC AGATGGCCGA CAAAATCATG GAGCTTGAAG AAAATTTGCG GGACTCGCAG 
GCGATGGCGC AGCGCATGGA AACACAGCTG GAGCAGAAGG AAAAGCTTTT TGAAGACAAG 
ATCAAGGTTT TAGAAACTCA AATGAAGGCT GATTTGGCTG AGAAGGATTT GCTGGAGTCC 
AAACAGAGCA CTTTTGAAGA GGAGTCTCGC GAACGGGGAA AAGTGATCAG CGACCAGGCA 
GCGACGATCA ATGCCATGGA GTCAAAGATG ACCAGTCTGG AGCAGAGGAT CGCTGAGCTG 
TCCGAAGCCA ACAAACTGGC AGCAAACAGC AGCATTTACA CACAGAAGAA CATGAAAGCC 
CAGGAGGAGA TGATTTCTGA ACTCAGACAG CAGAAGTTTT ATTTGGAGTC TCAGGCTGGA 
AAACTGGAGG CGCAGAATGC AAAGCTGGAG GAGCATCTGG AGAAACTCAC CCAACAAGAG 
CAGACAAACA AGAGCCGAGT GCTGGAGCTG GAAGCCAGGC TGCGAGAGAT CGGCCTAGAG 
CATGAGGAAC AGAAGCTGGA GATCAAGCGT CAGGTGTCGG AGCTCACGTT GTCTCTGCAG 
GAGAGAGAAG CTCAGATCAG CAGCCTGCAA GCGGCACGCC ACGCTCTCGA CAGCCAACTA 
CAGCAGGCCA AAACTGAGCT GGAGGAGACC ACCGCCGAGG CCGAAGAGGA GATCACCGTC 
CTGAGGGCCC ATCGGGATGA AATCCAACGC AAGTTTGATG CCCTGCGAGA CAGCTGTGCG 
GTGATCACGG ATCTGGAGGA GCAGTTGACG CAGCTCACGC AAGAAAACGC GGAGCTGAAC 
CGGCAGAACT TCTACCTGTC CAAACAGCTG GACGAGGCCA CAGACGAGAA CGAGGAGCGC 
CTGCAGCTCA AGCAGGACGT GGAGCGCCTC AGGAGAGAGG TGGCAGACCG AGAGATGCAC 
CTCAACAACC AGAAACAGAA CATCGAGACG CTGAAGACGA CATGTACAAT GCTGGAGGAG 
CAGGTTATGG AGCTGGAGAC TCTGAATGAT GAGCTGATGG AGAAGGAGAG GCAGTGGGAG 
AACTGGAGAT CTGCTCTGGA CGAGGAGAAA AACCAGGCCG AGCGCAGAGC CAGAGACCTG 
CAAAGACAGC TGGACAGCGA GAAGCAGAAC CGACTGCGTG CAGAACAGAG AAACTCTGAG 
TCTCGGCAGG CCGTGGAGCT GGCGGTGAAA GAGCACAAAG CTGAAATCCT GGTTCTGCAG 
CAGGCGCTCA AAGACCAGAA ACTCAAGGCT GAGGGCCTTT CTGACACTCT GGGTGATCTG 
GAGAAGAAGC ACGGCATGCT GGAGATGAAC GCTCGCAGCC TGCAGCAGAA GCTGGAGACG 
GAGAGAGAGA TGAAGCAGAG ACTCATGGAC GAGCAAGCTA AACTGCAGCA GCAGCTGGAC 
ATGCAGAAGA GCCACATCTT CAGACTGACT CAAGGACTGC AGGATGCGCT GGACCAGACC 
GACCTGCTGA AGACCGAGCG CACAGACCTG GAGTACCAGC TGGAAAACAT ACAGGCGGCG 
TATTCTCACG AGAAGGTGAA GATGGAGGGG ACGATCACCC AGCAGACCAA GCTCATAGAC 
TTCCTGCAGG CCAAGGTAGA GCACCCCTCT AAGAAGAAAA AGGGGCTGTT TGGTGGCCGG 
CGGCGGGAGG ATCTGCTAGC GGCGCTGGCG GCTCAGGGAC AGACACAGGT GCCTGTGGTG 
CCCGCCGCCA CTCCTGTGCC CCTGCAGTAC AGCGACCTGA AGGCGGCGCT GGATAAAGAG 
CGAGCCCGAT GCTCCGAGCT GGAGGACGCC CTGCAGAAAA CACGCCTGGA GCTCAGAGAA 
GTTCAGTTTA ATAAAGCTCT GGAGCACAGC TCTTCTCCAG CCACTCCAGG TTTGGCCCGT 
CAGCAGCTCA TGATGTCCAC CAAGTCCAAC AAGTCTCCAG AGCACCAGTC CAGCGGCGGC 
CTGATGTCCT CAAACTCCAG CCGCGGACGC AAAGACAACG CCAATCCTGA AGAGTACACC 
AGACGTGTGA AGGACCGGAT TCATCACAAC ACTCCTCACC GCTTCACGCA GGGGCTCAAT 
ATGAGAGCGG CTCGCTGTAC TGTGTGTCTG GACACAGTCC ATTTTGGACG TCAGGCTGCA 
ACATGCATTG AATGTCACGC GTTGTGTCAC CCAAAATGCT CCCCGTACCT CCCTGCCACC 
TGCGGCGTTC CCACTGAATA CGCGCTGCAC CTCTCAGAGG CTCTGTGTCG GGAGAAGGGA 
AGCACTTCCG GCCTGAAAGA TATGGGTGGA CATATGAGGC TTGAAGGATG GCTGAAGCAG 
CCCAGGAACG GGAAGCGCGG GCAGGGCTGG GAGAGGAAGT ATGTGGTGCT GGATGGATAT 
AAAGTGATTA CATTCGAGAC AGAACCAAGA GACGAGTCAG TGAAGCCGCT GGATGAGTTT 
GAGCTGTGTC TCTCTGATGG AGAAGTGACC GTTCATTCTG CTGTGGGTTC CTCTGAGCTC 
CCCAACACCG CTAAAACAGA TGTGCCGTAC GTGCTGAAGC TGGAGTCGTC TCCTCAGTCC 
ACATGCTGGC CCGGTCAGAC TCTGTACCTC ATGACCTCGG GATACTCAGA GAAGCAGCGC 
TGGGTGGCAG TGCTGGAAGC CATCGTGGCC AGCAGTCGCG GAGCCAGAGA GAAAGCTGAG 
GCAGATGCTA AATTATTGGG TAACTCTTTA CTTAAGCTAG AAGGTGACGA CCGTCTGGAT 
ATCAACTGCA CCCTGCCGCT GACCGACCAG ATTGTGTTGG TGGGCTCTGA GGAGGGTCTC 
TACGCACTGA ATGTGATTAA GAACTCCCTC ACTCATATCC CGGGTCTGGG ATCCGTGTTT 
CAGATCCACA TTATCAAAGA TCATGACAAG CTGCTGATGA TCGTGGGAGA CGAGCGAGCG 
CTGTGTCTGG TGGAGATTAA GAAGGTGAAG CAGTCTTTAT CCCAGTCTCA CCTCCCCACA 
CTGCCCGAGA TTGTGCCGTA TATCTTTGAG ACCGTCAAGG GCTGTCATCT GTTTGCCTCG 
GGCAGGATAG AAAATGGCCC ATGCATTTGT GCTGCGATGC CCAATAAAGT CACGATTCTA 
CGATACAATG ACAATCTTAA CAAGTTCTGC ATACGGAAGG AGATTGAAAC TCTGGAGCCG 
TGCAGCTGCA TCCACTTCAC CAGCTACAGC ATCATCATTG GTACCAATAA ATTCTACGAG 
ATCGAGATGA AGCAGTGCGT TCTGGACGAG TTCCTGGACA AGAATGACGT GTCGCTGGCG 
TCGGCTGTCT TCACCGGATC CTCCCACAGT TTCCCCATCT CTATCGTCCA GGTGTCCAGC 
ACTCCGCAGA AGGAGGAATA TCTGCTGTGT TTCCACGAGA TCGGAGTTTT TGTGGATGCT 
TACGGTCGAA GAAGTCGCCC TGATGATCTG AAGTGGAGTC GACTTCCCCT GACGTTCGCC 
TTCAGAGAGC CGTATCTCTT TGTGACCTAC TTCAACTCTC TGGATGTTAT TGAGATTCAG 
GGACACGCAG CTCTTGGGCC TCCCAGTCTG GTTCACCTGG ACATTCCTAA CCCACGTTAT 
CTGGGTCCAG CCATATCGTC TGGTGCCATC TATCTGGCCT CGTCCTACCA GAACAAACTG 
CGGGTGATCT GCTGTAAGGG CAGTCTGGTG CGGGAGTCTG GAGAGCTGCA GAGGACCGGC 
TCCAGCAGGA GCAGCCCCAA CAAGCGCGGT CCACCAACAT ACAACGAGCA CATCTCCAAA 
CGTCTGGCCT CGAGTCCTGG AGCCCAGGAG GGGCCCCTGC ACCGCGAGCC CAGCACTCCT 
CACCGCTACC GCGAGGGCCG CACCGAGTTC CGGAGAGACA AATCTCCAGC CCGGCCCCTG 
GAGCGAGAGA AATCCCCGGG CCGTCCGGTG CTAGACAGCC GCAGAGAGAG GTCTCCCGGT 
CGCTTCGAGA GCAGCGGCAG CGAGAGAGAC CGCGAGCGGA CACGACTGCA CACTAGCTCT 
GTACGCACCC AGCTGACCCC CGTCAACAAG GTTTGGGACC AGTCTTCGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100333880 ( XM_017357405.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212894.1 Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X11, mRNA.

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CloneID	ODa60013
Clone ID Related Accession (Same CDS sequence)	XM_017357405.1 , XM_017357405.2
Accession Version	XM_017357405.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	citron Rho-interacting kinase-like isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG AGATTTCCAA AGTGCAGCGT AAGATGTCAG ACTTGGAGTC AGTTTTGCAG 
CAGAAAGACG TGGAGCTCAA GGCTTCAGAA ACCCAGAGAA ACATCTTAGA GCAAGACCTG 
GCAACCTATA TCACCGAATG CAGCAGTTTG AAGCGCAGTT TGGAGCAGGC CCGCATGGAG 
GTTTCGCAGG AAGATGATAA AGCTCTTCAG CTGCTGCACG ACATTCGAGA GCAGAGCAAC 
AAACTGCAGG AAATCAAAGA ACAGGAGTAC CACGCTCAGC TGGAGGAGAT GCGAGTGTCT 
ATTCGTCAGC TGGAAGAGAA TCTGTCGGCC GCTCGTCGCC GCAGTGACCT GTACGAGTCT 
GAGCTCAAAG AGTCCCGTCA GACCAGTGAG GAGCTCAAGA GGAAGGCAGT GGAGTATCAG 
CAGAAGATCC AGAAGGCTAA AGAACAAGGG AAAGTTGAGA TGGAGGAGCT TCTGAGCAAA 
CTGCAGAAGA CCAATGGAGA GCAACAAGTA AAGATTGAAG AGCTCCAGGA GAAGCTTGCT 
AAGGCAGTGA AGGCGAGTAC TGAAGCCACT GAACTCCTCC AGAATGTCCG TCAGGCTAAA 
GAGCGAATGG AGCGAGACCT GGAGCGTCTG CAGACCAAAG AGGACTCGTC CGACAGCCTG 
CGCAGACGCT TACGAGAGAC TGAGGATGGC AAAAAATCCC TGGAGAACCA GGTGAAGAGG 
CTGGAGATGG TGGAGCGGAG AGAATCCAAA CTGAAAGACG ACATTCAGAC CAAATCGCAG 
CAGATCCAGC AGATGGCCGA CAAAATCATG GAGCTTGAAG AAAATTTGCG GGACTCGCAG 
GCGATGGCGC AGCGCATGGA AACACAGCTG GAGCAGAAGG AAAAGCTTTT TGAAGACAAG 
ATCAAGGTTT TAGAAACTCA AATGAAGGCT GATTTGGCTG AGAAGGATTT GCTGGAGTCC 
AAACAGAGCA CTTTTGAAGA GGAGTCTCGC GAACGGGGAA AAGTGATCAG CGACCAGGCA 
GCGACGATCA ATGCCATGGA GTCAAAGATG ACCAGTCTGG AGCAGAGGAT CGCTGAGCTG 
TCCGAAGCCA ACAAACTGGC AGCAAACAGC AGCATTTACA CACAGAAGAA CATGAAAGCC 
CAGGAGGAGA TGATTTCTGA ACTCAGACAG CAGAAGTTTT ATTTGGAGTC TCAGGCTGGA 
AAACTGGAGG CGCAGAATGC AAAGCTGGAG GAGCATCTGG AGAAACTCAC CCAACAAGAG 
CAGACAAACA AGAGCCGAGT GCTGGAGCTG GAAGCCAGGC TGCGAGAGAT CGGCCTAGAG 
CATGAGGAAC AGAAGCTGGA GATCAAGCGT CAGGTGTCGG AGCTCACGTT GTCTCTGCAG 
GAGAGAGAAG CTCAGATCAG CAGCCTGCAA GCGGCACGCC ACGCTCTCGA CAGCCAACTA 
CAGCAGGCCA AAACTGAGCT GGAGGAGACC ACCGCCGAGG CCGAAGAGGA GATCACCGTC 
CTGAGGGCCC ATCGGGATGA AATCCAACGC AAGTTTGATG CCCTGCGAGA CAGCTGTGCG 
GTGATCACGG ATCTGGAGGA GCAGTTGACG CAGCTCACGC AAGAAAACGC GGAGCTGAAC 
CGGCAGAACT TCTACCTGTC CAAACAGCTG GACGAGGCCA CAGACGAGAA CGAGGAGCGC 
CTGCAGCTCA AGCAGGACGT GGAGCGCCTC AGGAGAGAGG TGGCAGACCG AGAGATGCAC 
CTCAACAACC AGAAACAGAA CATCGAGACG CTGAAGACGA CATGTACAAT GCTGGAGGAG 
CAGGTTATGG AGCTGGAGAC TCTGAATGAT GAGCTGATGG AGAAGGAGAG GCAGTGGGAG 
AACTGGAGAT CTGCTCTGGA CGAGGAGAAA AACCAGGCCG AGCGCAGAGC CAGAGACCTG 
CAAAGACAGC TGGACAGCGA GAAGCAGAAC CGACTGCGTG CAGAACAGAG AAACTCTGAG 
TCTCGGCAGG CCGTGGAGCT GGCGGTGAAA GAGCACAAAG CTGAAATCCT GGTTCTGCAG 
CAGGCGCTCA AAGACCAGAA ACTCAAGGCT GAGGGCCTTT CTGACACTCT GGGTGATCTG 
GAGAAGAAGC ACGGCATGCT GGAGATGAAC GCTCGCAGCC TGCAGCAGAA GCTGGAGACG 
GAGAGAGAGA TGAAGCAGAG ACTCATGGAC GAGCAAGCTA AACTGCAGCA GCAGCTGGAC 
ATGCAGAAGA GCCACATCTT CAGACTGACT CAAGGACTGC AGGATGCGCT GGACCAGACC 
GACCTGCTGA AGACCGAGCG CACAGACCTG GAGTACCAGC TGGAAAACAT ACAGGCGGCG 
TATTCTCACG AGAAGGTGAA GATGGAGGGG ACGATCACCC AGCAGACCAA GCTCATAGAC 
TTCCTGCAGG CCAAGGTAGA GCACCCCTCT AAGAAGAAAA AGGGGCTGTT TGGTGGCCGG 
CGGCGGGAGG ATCTGCTAGC GGCGCTGGCG GCTCAGGGAC AGACACAGGT GCCTGTGGTG 
CCCGCCGCCA CTCCTGTGCC CCTGCAGTAC AGCGACCTGA AGGCGGCGCT GGATAAAGAG 
CGAGCCCGAT GCTCCGAGCT GGAGGACGCC CTGCAGAAAA CACGCCTGGA GCTCAGAGAA 
GTTCAGTTTA ATAAAGCTCT GGAGCACAGC TCTTCTCCAG CCACTCCAGG TTTGGCCCGT 
CAGCAGCTCA TGATGTCCAC CAAGTCCAAC AAGTCTCCAG AGCACCAGTC CAGCGGCGGC 
CTGATGTCCT CAAACTCCAG CCGCGGACGC AAAGACAACG CCAATCCTGA AGAGTACACC 
AGACGTGTGA AGGACCGGAT TCATCACAAC ACTCCTCACC GCTTCACGCA GGGGCTCAAT 
ATGAGAGCGG CTCGCTGTAC TGTGTGTCTG GACACAGTCC ATTTTGGACG TCAGGCTGCA 
ACATGCATTG AATGTCACGC GTTGTGTCAC CCAAAATGCT CCCCGTACCT CCCTGCCACC 
TGCGGCGTTC CCACTGAATA CGCGCTGCAC CTCTCAGAGG CTCTGTGTCG GGAGAAGGGA 
AGCACTTCCG GCCTGAAAGA TATGGGTGGA CATATGAGGC TTGAAGGATG GCTGAAGCAG 
CCCAGGAACG GGAAGCGCGG GCAGGGCTGG GAGAGGAAGT ATGTGGTGCT GGATGGATAT 
AAAGTGATTA CATTCGAGAC AGAACCAAGA GACGAGTCAG TGAAGCCGCT GGATGAGTTT 
GAGCTGTGTC TCTCTGATGG AGAAGTGACC GTTCATTCTG CTGTGGGTTC CTCTGAGCTC 
CCCAACACCG CTAAAACAGA TGTGCCGTAC GTGCTGAAGC TGGAGTCGTC TCCTCAGTCC 
ACATGCTGGC CCGGTCAGAC TCTGTACCTC ATGACCTCGG GATACTCAGA GAAGCAGCGC 
TGGGTGGCAG TGCTGGAAGC CATCGTGGCC AGCAGTCGCG GAGCCAGAGA GAAAGCTGAG 
GCAGATGCTA AATTATTGGG TAACTCTTTA CTTAAGCTAG AAGGTGACGA CCGTCTGGAT 
ATCAACTGCA CCCTGCCGCT GACCGACCAG ATTGTGTTGG TGGGCTCTGA GGAGGGTCTC 
TACGCACTGA ATGTGATTAA GAACTCCCTC ACTCATATCC CGGGTCTGGG ATCCGTGTTT 
CAGATCCACA TTATCAAAGA TCATGACAAG CTGCTGATGA TCGTGGGAGA CGAGCGAGCG 
CTGTGTCTGG TGGAGATTAA GAAGGTGAAG CAGTCTTTAT CCCAGTCTCA CCTCCCCACA 
CTGCCCGAGA TTGTGCCGTA TATCTTTGAG ACCGTCAAGG GCTGTCATCT GTTTGCCTCG 
GGCAGGATAG AAAATGGCCC ATGCATTTGT GCTGCGATGC CCAATAAAGT CACGATTCTA 
CGATACAATG ACAATCTTAA CAAGTTCTGC ATACGGAAGG AGATTGAAAC TCTGGAGCCG 
TGCAGCTGCA TCCACTTCAC CAGCTACAGC ATCATCATTG GTACCAATAA ATTCTACGAG 
ATCGAGATGA AGCAGTGCGT TCTGGACGAG TTCCTGGACA AGAATGACGT GTCGCTGGCG 
TCGGCTGTCT TCACCGGATC CTCCCACAGT TTCCCCATCT CTATCGTCCA GGTGTCCAGC 
ACTCCGCAGA AGGAGGAATA TCTGCTGTGT TTCCACGAGA TCGGAGTTTT TGTGGATGCT 
TACGGTCGAA GAAGTCGCCC TGATGATCTG AAGTGGAGTC GACTTCCCCT GACGTTCGCC 
TTCAGAGAGC CGTATCTCTT TGTGACCTAC TTCAACTCTC TGGATGTTAT TGAGATTCAG 
GGACACGCAG CTCTTGGGCC TCCCAGTCTG GTTCACCTGG ACATTCCTAA CCCACGTTAT 
CTGGGTCCAG CCATATCGTC TGGTGCCATC TATCTGGCCT CGTCCTACCA GAACAAACTG 
CGGGTGATCT GCTGTAAGGG CAGTCTGGTG CGGGAGTCTG GAGAGCTGCA GAGGACCGGC 
TCCAGCAGGA GCCCCAACAA GCGCGGTCCA CCAACATACA ACGAGCACAT CTCCAAACGT 
CTGGCCTCGA GTCCTGGAGC CCAGGAGGGG CCCCTGCACC GCGAGCCCAG CACTCCTCAC 
CGCTACCGCG AGGGCCGCAC CGAGTTCCGG AGAGACAAAT CTCCAGCCCG GCCCCTGGAG 
CGAGAGAAAT CCCCGGGCCG TCCGGTGCTA GACAGCCGCA GAGAGAGGTC TCCCGGTCGC 
TTCGAGAGCA GCGGCAGCGA GAGAGACCGC GAGCGGACAC GACTGCACAC TAGCTCTGTA 
CGCACCCAGC TGACCCCCGT CAACAAGGTT TGGGACCAGT CTTCGGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212894.1
CDS	434..5284
Translation	MEQEISKVQRKMSDLESVLQQKDVELKASETQRNILEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSNKLQEIKEQEYHAQLEEMRVSIRQLEENLSAARRRSDLYESELKESRQTSEELKRKAVEYQQKIQKAKEQGKVEMEELLSKLQKTNGEQQVKIEELQEKLAKAVKASTEATELLQNVRQAKERMERDLERLQTKEDSSDSLRRRLRETEDGKKSLENQVKRLEMVERRESKLKDDIQTKSQQIQQMADKIMELEENLRDSQAMAQRMETQLEQKEKLFEDKIKVLETQMKADLAEKDLLESKQSTFEEESRERGKVISDQAATINAMESKMTSLEQRIAELSEANKLAANSSIYTQKNMKAQEEMISELRQQKFYLESQAGKLEAQNAKLEEHLEKLTQQEQTNKSRVLELEARLREIGLEHEEQKLEIKRQVSELTLSLQEREAQISSLQAARHALDSQLQQAKTELEETTAEAEEEITVLRAHRDEIQRKFDALRDSCAVITDLEEQLTQLTQENAELNRQNFYLSKQLDEATDENEERLQLKQDVERLRREVADREMHLNNQKQNIETLKTTCTMLEEQVMELETLNDELMEKERQWENWRSALDEEKNQAERRARDLQRQLDSEKQNRLRAEQRNSESRQAVELAVKEHKAEILVLQQALKDQKLKAEGLSDTLGDLEKKHGMLEMNARSLQQKLETEREMKQRLMDEQAKLQQQLDMQKSHIFRLTQGLQDALDQTDLLKTERTDLEYQLENIQAAYSHEKVKMEGTITQQTKLIDFLQAKVEHPSKKKKGLFGGRRREDLLAALAAQGQTQVPVVPAATPVPLQYSDLKAALDKERARCSELEDALQKTRLELREVQFNKALEHSSSPATPGLARQQLMMSTKSNKSPEHQSSGGLMSSNSSRGRKDNANPEEYTRRVKDRIHHNTPHRFTQGLNMRAARCTVCLDTVHFGRQAATCIECHALCHPKCSPYLPATCGVPTEYALHLSEALCREKGSTSGLKDMGGHMRLEGWLKQPRNGKRGQGWERKYVVLDGYKVITFETEPRDESVKPLDEFELCLSDGEVTVHSAVGSSELPNTAKTDVPYVLKLESSPQSTCWPGQTLYLMTSGYSEKQRWVAVLEAIVASSRGAREKAEADAKLLGNSLLKLEGDDRLDINCTLPLTDQIVLVGSEEGLYALNVIKNSLTHIPGLGSVFQIHIIKDHDKLLMIVGDERALCLVEIKKVKQSLSQSHLPTLPEIVPYIFETVKGCHLFASGRIENGPCICAAMPNKVTILRYNDNLNKFCIRKEIETLEPCSCIHFTSYSIIIGTNKFYEIEMKQCVLDEFLDKNDVSLASAVFTGSSHSFPISIVQVSSTPQKEEYLLCFHEIGVFVDAYGRRSRPDDLKWSRLPLTFAFREPYLFVTYFNSLDVIEIQGHAALGPPSLVHLDIPNPRYLGPAISSGAIYLASSYQNKLRVICCKGSLVRESGELQRTGSSRSPNKRGPPTYNEHISKRLASSPGAQEGPLHREPSTPHRYREGRTEFRRDKSPARPLEREKSPGRPVLDSRRERSPGRFESSGSERDRERTRLHTSSVRTQLTPVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_017357405.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357405.1

ORF Insert Sequence:

ATGGAGCAGG AGATTTCCAA AGTGCAGCGT AAGATGTCAG ACTTGGAGTC AGTTTTGCAG 
CAGAAAGACG TGGAGCTCAA GGCTTCAGAA ACCCAGAGAA ACATCTTAGA GCAAGACCTG 
GCAACCTATA TCACCGAATG CAGCAGTTTG AAGCGCAGTT TGGAGCAGGC CCGCATGGAG 
GTTTCGCAGG AAGATGATAA AGCTCTTCAG CTGCTGCACG ACATTCGAGA GCAGAGCAAC 
AAACTGCAGG AAATCAAAGA ACAGGAGTAC CACGCTCAGC TGGAGGAGAT GCGAGTGTCT 
ATTCGTCAGC TGGAAGAGAA TCTGTCGGCC GCTCGTCGCC GCAGTGACCT GTACGAGTCT 
GAGCTCAAAG AGTCCCGTCA GACCAGTGAG GAGCTCAAGA GGAAGGCAGT GGAGTATCAG 
CAGAAGATCC AGAAGGCTAA AGAACAAGGG AAAGTTGAGA TGGAGGAGCT TCTGAGCAAA 
CTGCAGAAGA CCAATGGAGA GCAACAAGTA AAGATTGAAG AGCTCCAGGA GAAGCTTGCT 
AAGGCAGTGA AGGCGAGTAC TGAAGCCACT GAACTCCTCC AGAATGTCCG TCAGGCTAAA 
GAGCGAATGG AGCGAGACCT GGAGCGTCTG CAGACCAAAG AGGACTCGTC CGACAGCCTG 
CGCAGACGCT TACGAGAGAC TGAGGATGGC AAAAAATCCC TGGAGAACCA GGTGAAGAGG 
CTGGAGATGG TGGAGCGGAG AGAATCCAAA CTGAAAGACG ACATTCAGAC CAAATCGCAG 
CAGATCCAGC AGATGGCCGA CAAAATCATG GAGCTTGAAG AAAATTTGCG GGACTCGCAG 
GCGATGGCGC AGCGCATGGA AACACAGCTG GAGCAGAAGG AAAAGCTTTT TGAAGACAAG 
ATCAAGGTTT TAGAAACTCA AATGAAGGCT GATTTGGCTG AGAAGGATTT GCTGGAGTCC 
AAACAGAGCA CTTTTGAAGA GGAGTCTCGC GAACGGGGAA AAGTGATCAG CGACCAGGCA 
GCGACGATCA ATGCCATGGA GTCAAAGATG ACCAGTCTGG AGCAGAGGAT CGCTGAGCTG 
TCCGAAGCCA ACAAACTGGC AGCAAACAGC AGCATTTACA CACAGAAGAA CATGAAAGCC 
CAGGAGGAGA TGATTTCTGA ACTCAGACAG CAGAAGTTTT ATTTGGAGTC TCAGGCTGGA 
AAACTGGAGG CGCAGAATGC AAAGCTGGAG GAGCATCTGG AGAAACTCAC CCAACAAGAG 
CAGACAAACA AGAGCCGAGT GCTGGAGCTG GAAGCCAGGC TGCGAGAGAT CGGCCTAGAG 
CATGAGGAAC AGAAGCTGGA GATCAAGCGT CAGGTGTCGG AGCTCACGTT GTCTCTGCAG 
GAGAGAGAAG CTCAGATCAG CAGCCTGCAA GCGGCACGCC ACGCTCTCGA CAGCCAACTA 
CAGCAGGCCA AAACTGAGCT GGAGGAGACC ACCGCCGAGG CCGAAGAGGA GATCACCGTC 
CTGAGGGCCC ATCGGGATGA AATCCAACGC AAGTTTGATG CCCTGCGAGA CAGCTGTGCG 
GTGATCACGG ATCTGGAGGA GCAGTTGACG CAGCTCACGC AAGAAAACGC GGAGCTGAAC 
CGGCAGAACT TCTACCTGTC CAAACAGCTG GACGAGGCCA CAGACGAGAA CGAGGAGCGC 
CTGCAGCTCA AGCAGGACGT GGAGCGCCTC AGGAGAGAGG TGGCAGACCG AGAGATGCAC 
CTCAACAACC AGAAACAGAA CATCGAGACG CTGAAGACGA CATGTACAAT GCTGGAGGAG 
CAGGTTATGG AGCTGGAGAC TCTGAATGAT GAGCTGATGG AGAAGGAGAG GCAGTGGGAG 
AACTGGAGAT CTGCTCTGGA CGAGGAGAAA AACCAGGCCG AGCGCAGAGC CAGAGACCTG 
CAAAGACAGC TGGACAGCGA GAAGCAGAAC CGACTGCGTG CAGAACAGAG AAACTCTGAG 
TCTCGGCAGG CCGTGGAGCT GGCGGTGAAA GAGCACAAAG CTGAAATCCT GGTTCTGCAG 
CAGGCGCTCA AAGACCAGAA ACTCAAGGCT GAGGGCCTTT CTGACACTCT GGGTGATCTG 
GAGAAGAAGC ACGGCATGCT GGAGATGAAC GCTCGCAGCC TGCAGCAGAA GCTGGAGACG 
GAGAGAGAGA TGAAGCAGAG ACTCATGGAC GAGCAAGCTA AACTGCAGCA GCAGCTGGAC 
ATGCAGAAGA GCCACATCTT CAGACTGACT CAAGGACTGC AGGATGCGCT GGACCAGACC 
GACCTGCTGA AGACCGAGCG CACAGACCTG GAGTACCAGC TGGAAAACAT ACAGGCGGCG 
TATTCTCACG AGAAGGTGAA GATGGAGGGG ACGATCACCC AGCAGACCAA GCTCATAGAC 
TTCCTGCAGG CCAAGGTAGA GCACCCCTCT AAGAAGAAAA AGGGGCTGTT TGGTGGCCGG 
CGGCGGGAGG ATCTGCTAGC GGCGCTGGCG GCTCAGGGAC AGACACAGGT GCCTGTGGTG 
CCCGCCGCCA CTCCTGTGCC CCTGCAGTAC AGCGACCTGA AGGCGGCGCT GGATAAAGAG 
CGAGCCCGAT GCTCCGAGCT GGAGGACGCC CTGCAGAAAA CACGCCTGGA GCTCAGAGAA 
GTTCAGTTTA ATAAAGCTCT GGAGCACAGC TCTTCTCCAG CCACTCCAGG TTTGGCCCGT 
CAGCAGCTCA TGATGTCCAC CAAGTCCAAC AAGTCTCCAG AGCACCAGTC CAGCGGCGGC 
CTGATGTCCT CAAACTCCAG CCGCGGACGC AAAGACAACG CCAATCCTGA AGAGTACACC 
AGACGTGTGA AGGACCGGAT TCATCACAAC ACTCCTCACC GCTTCACGCA GGGGCTCAAT 
ATGAGAGCGG CTCGCTGTAC TGTGTGTCTG GACACAGTCC ATTTTGGACG TCAGGCTGCA 
ACATGCATTG AATGTCACGC GTTGTGTCAC CCAAAATGCT CCCCGTACCT CCCTGCCACC 
TGCGGCGTTC CCACTGAATA CGCGCTGCAC CTCTCAGAGG CTCTGTGTCG GGAGAAGGGA 
AGCACTTCCG GCCTGAAAGA TATGGGTGGA CATATGAGGC TTGAAGGATG GCTGAAGCAG 
CCCAGGAACG GGAAGCGCGG GCAGGGCTGG GAGAGGAAGT ATGTGGTGCT GGATGGATAT 
AAAGTGATTA CATTCGAGAC AGAACCAAGA GACGAGTCAG TGAAGCCGCT GGATGAGTTT 
GAGCTGTGTC TCTCTGATGG AGAAGTGACC GTTCATTCTG CTGTGGGTTC CTCTGAGCTC 
CCCAACACCG CTAAAACAGA TGTGCCGTAC GTGCTGAAGC TGGAGTCGTC TCCTCAGTCC 
ACATGCTGGC CCGGTCAGAC TCTGTACCTC ATGACCTCGG GATACTCAGA GAAGCAGCGC 
TGGGTGGCAG TGCTGGAAGC CATCGTGGCC AGCAGTCGCG GAGCCAGAGA GAAAGCTGAG 
GCAGATGCTA AATTATTGGG TAACTCTTTA CTTAAGCTAG AAGGTGACGA CCGTCTGGAT 
ATCAACTGCA CCCTGCCGCT GACCGACCAG ATTGTGTTGG TGGGCTCTGA GGAGGGTCTC 
TACGCACTGA ATGTGATTAA GAACTCCCTC ACTCATATCC CGGGTCTGGG ATCCGTGTTT 
CAGATCCACA TTATCAAAGA TCATGACAAG CTGCTGATGA TCGTGGGAGA CGAGCGAGCG 
CTGTGTCTGG TGGAGATTAA GAAGGTGAAG CAGTCTTTAT CCCAGTCTCA CCTCCCCACA 
CTGCCCGAGA TTGTGCCGTA TATCTTTGAG ACCGTCAAGG GCTGTCATCT GTTTGCCTCG 
GGCAGGATAG AAAATGGCCC ATGCATTTGT GCTGCGATGC CCAATAAAGT CACGATTCTA 
CGATACAATG ACAATCTTAA CAAGTTCTGC ATACGGAAGG AGATTGAAAC TCTGGAGCCG 
TGCAGCTGCA TCCACTTCAC CAGCTACAGC ATCATCATTG GTACCAATAA ATTCTACGAG 
ATCGAGATGA AGCAGTGCGT TCTGGACGAG TTCCTGGACA AGAATGACGT GTCGCTGGCG 
TCGGCTGTCT TCACCGGATC CTCCCACAGT TTCCCCATCT CTATCGTCCA GGTGTCCAGC 
ACTCCGCAGA AGGAGGAATA TCTGCTGTGT TTCCACGAGA TCGGAGTTTT TGTGGATGCT 
TACGGTCGAA GAAGTCGCCC TGATGATCTG AAGTGGAGTC GACTTCCCCT GACGTTCGCC 
TTCAGAGAGC CGTATCTCTT TGTGACCTAC TTCAACTCTC TGGATGTTAT TGAGATTCAG 
GGACACGCAG CTCTTGGGCC TCCCAGTCTG GTTCACCTGG ACATTCCTAA CCCACGTTAT 
CTGGGTCCAG CCATATCGTC TGGTGCCATC TATCTGGCCT CGTCCTACCA GAACAAACTG 
CGGGTGATCT GCTGTAAGGG CAGTCTGGTG CGGGAGTCTG GAGAGCTGCA GAGGACCGGC 
TCCAGCAGGA GCCCCAACAA GCGCGGTCCA CCAACATACA ACGAGCACAT CTCCAAACGT 
CTGGCCTCGA GTCCTGGAGC CCAGGAGGGG CCCCTGCACC GCGAGCCCAG CACTCCTCAC 
CGCTACCGCG AGGGCCGCAC CGAGTTCCGG AGAGACAAAT CTCCAGCCCG GCCCCTGGAG 
CGAGAGAAAT CCCCGGGCCG TCCGGTGCTA GACAGCCGCA GAGAGAGGTC TCCCGGTCGC 
TTCGAGAGCA GCGGCAGCGA GAGAGACCGC GAGCGGACAC GACTGCACAC TAGCTCTGTA 
CGCACCCAGC TGACCCCCGT CAACAAGGTT TGGGACCAGT CTTCGGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100333880 ( XM_017357405.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212894.1 Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X13, mRNA.

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CloneID	ODa60013
Clone ID Related Accession (Same CDS sequence)	XM_017357405.1 , XM_017357405.2
Accession Version	XM_017357405.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	citron Rho-interacting kinase-like isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017357405.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG AGATTTCCAA AGTGCAGCGT AAGATGTCAG ACTTGGAGTC AGTTTTGCAG 
CAGAAAGACG TGGAGCTCAA GGCTTCAGAA ACCCAGAGAA ACATCTTAGA GCAAGACCTG 
GCAACCTATA TCACCGAATG CAGCAGTTTG AAGCGCAGTT TGGAGCAGGC CCGCATGGAG 
GTTTCGCAGG AAGATGATAA AGCTCTTCAG CTGCTGCACG ACATTCGAGA GCAGAGCAAC 
AAACTGCAGG AAATCAAAGA ACAGGAGTAC CACGCTCAGC TGGAGGAGAT GCGAGTGTCT 
ATTCGTCAGC TGGAAGAGAA TCTGTCGGCC GCTCGTCGCC GCAGTGACCT GTACGAGTCT 
GAGCTCAAAG AGTCCCGTCA GACCAGTGAG GAGCTCAAGA GGAAGGCAGT GGAGTATCAG 
CAGAAGATCC AGAAGGCTAA AGAACAAGGG AAAGTTGAGA TGGAGGAGCT TCTGAGCAAA 
CTGCAGAAGA CCAATGGAGA GCAACAAGTA AAGATTGAAG AGCTCCAGGA GAAGCTTGCT 
AAGGCAGTGA AGGCGAGTAC TGAAGCCACT GAACTCCTCC AGAATGTCCG TCAGGCTAAA 
GAGCGAATGG AGCGAGACCT GGAGCGTCTG CAGACCAAAG AGGACTCGTC CGACAGCCTG 
CGCAGACGCT TACGAGAGAC TGAGGATGGC AAAAAATCCC TGGAGAACCA GGTGAAGAGG 
CTGGAGATGG TGGAGCGGAG AGAATCCAAA CTGAAAGACG ACATTCAGAC CAAATCGCAG 
CAGATCCAGC AGATGGCCGA CAAAATCATG GAGCTTGAAG AAAATTTGCG GGACTCGCAG 
GCGATGGCGC AGCGCATGGA AACACAGCTG GAGCAGAAGG AAAAGCTTTT TGAAGACAAG 
ATCAAGGTTT TAGAAACTCA AATGAAGGCT GATTTGGCTG AGAAGGATTT GCTGGAGTCC 
AAACAGAGCA CTTTTGAAGA GGAGTCTCGC GAACGGGGAA AAGTGATCAG CGACCAGGCA 
GCGACGATCA ATGCCATGGA GTCAAAGATG ACCAGTCTGG AGCAGAGGAT CGCTGAGCTG 
TCCGAAGCCA ACAAACTGGC AGCAAACAGC AGCATTTACA CACAGAAGAA CATGAAAGCC 
CAGGAGGAGA TGATTTCTGA ACTCAGACAG CAGAAGTTTT ATTTGGAGTC TCAGGCTGGA 
AAACTGGAGG CGCAGAATGC AAAGCTGGAG GAGCATCTGG AGAAACTCAC CCAACAAGAG 
CAGACAAACA AGAGCCGAGT GCTGGAGCTG GAAGCCAGGC TGCGAGAGAT CGGCCTAGAG 
CATGAGGAAC AGAAGCTGGA GATCAAGCGT CAGGTGTCGG AGCTCACGTT GTCTCTGCAG 
GAGAGAGAAG CTCAGATCAG CAGCCTGCAA GCGGCACGCC ACGCTCTCGA CAGCCAACTA 
CAGCAGGCCA AAACTGAGCT GGAGGAGACC ACCGCCGAGG CCGAAGAGGA GATCACCGTC 
CTGAGGGCCC ATCGGGATGA AATCCAACGC AAGTTTGATG CCCTGCGAGA CAGCTGTGCG 
GTGATCACGG ATCTGGAGGA GCAGTTGACG CAGCTCACGC AAGAAAACGC GGAGCTGAAC 
CGGCAGAACT TCTACCTGTC CAAACAGCTG GACGAGGCCA CAGACGAGAA CGAGGAGCGC 
CTGCAGCTCA AGCAGGACGT GGAGCGCCTC AGGAGAGAGG TGGCAGACCG AGAGATGCAC 
CTCAACAACC AGAAACAGAA CATCGAGACG CTGAAGACGA CATGTACAAT GCTGGAGGAG 
CAGGTTATGG AGCTGGAGAC TCTGAATGAT GAGCTGATGG AGAAGGAGAG GCAGTGGGAG 
AACTGGAGAT CTGCTCTGGA CGAGGAGAAA AACCAGGCCG AGCGCAGAGC CAGAGACCTG 
CAAAGACAGC TGGACAGCGA GAAGCAGAAC CGACTGCGTG CAGAACAGAG AAACTCTGAG 
TCTCGGCAGG CCGTGGAGCT GGCGGTGAAA GAGCACAAAG CTGAAATCCT GGTTCTGCAG 
CAGGCGCTCA AAGACCAGAA ACTCAAGGCT GAGGGCCTTT CTGACACTCT GGGTGATCTG 
GAGAAGAAGC ACGGCATGCT GGAGATGAAC GCTCGCAGCC TGCAGCAGAA GCTGGAGACG 
GAGAGAGAGA TGAAGCAGAG ACTCATGGAC GAGCAAGCTA AACTGCAGCA GCAGCTGGAC 
ATGCAGAAGA GCCACATCTT CAGACTGACT CAAGGACTGC AGGATGCGCT GGACCAGACC 
GACCTGCTGA AGACCGAGCG CACAGACCTG GAGTACCAGC TGGAAAACAT ACAGGCGGCG 
TATTCTCACG AGAAGGTGAA GATGGAGGGG ACGATCACCC AGCAGACCAA GCTCATAGAC 
TTCCTGCAGG CCAAGGTAGA GCACCCCTCT AAGAAGAAAA AGGGGCTGTT TGGTGGCCGG 
CGGCGGGAGG ATCTGCTAGC GGCGCTGGCG GCTCAGGGAC AGACACAGGT GCCTGTGGTG 
CCCGCCGCCA CTCCTGTGCC CCTGCAGTAC AGCGACCTGA AGGCGGCGCT GGATAAAGAG 
CGAGCCCGAT GCTCCGAGCT GGAGGACGCC CTGCAGAAAA CACGCCTGGA GCTCAGAGAA 
GTTCAGTTTA ATAAAGCTCT GGAGCACAGC TCTTCTCCAG CCACTCCAGG TTTGGCCCGT 
CAGCAGCTCA TGATGTCCAC CAAGTCCAAC AAGTCTCCAG AGCACCAGTC CAGCGGCGGC 
CTGATGTCCT CAAACTCCAG CCGCGGACGC AAAGACAACG CCAATCCTGA AGAGTACACC 
AGACGTGTGA AGGACCGGAT TCATCACAAC ACTCCTCACC GCTTCACGCA GGGGCTCAAT 
ATGAGAGCGG CTCGCTGTAC TGTGTGTCTG GACACAGTCC ATTTTGGACG TCAGGCTGCA 
ACATGCATTG AATGTCACGC GTTGTGTCAC CCAAAATGCT CCCCGTACCT CCCTGCCACC 
TGCGGCGTTC CCACTGAATA CGCGCTGCAC CTCTCAGAGG CTCTGTGTCG GGAGAAGGGA 
AGCACTTCCG GCCTGAAAGA TATGGGTGGA CATATGAGGC TTGAAGGATG GCTGAAGCAG 
CCCAGGAACG GGAAGCGCGG GCAGGGCTGG GAGAGGAAGT ATGTGGTGCT GGATGGATAT 
AAAGTGATTA CATTCGAGAC AGAACCAAGA GACGAGTCAG TGAAGCCGCT GGATGAGTTT 
GAGCTGTGTC TCTCTGATGG AGAAGTGACC GTTCATTCTG CTGTGGGTTC CTCTGAGCTC 
CCCAACACCG CTAAAACAGA TGTGCCGTAC GTGCTGAAGC TGGAGTCGTC TCCTCAGTCC 
ACATGCTGGC CCGGTCAGAC TCTGTACCTC ATGACCTCGG GATACTCAGA GAAGCAGCGC 
TGGGTGGCAG TGCTGGAAGC CATCGTGGCC AGCAGTCGCG GAGCCAGAGA GAAAGCTGAG 
GCAGATGCTA AATTATTGGG TAACTCTTTA CTTAAGCTAG AAGGTGACGA CCGTCTGGAT 
ATCAACTGCA CCCTGCCGCT GACCGACCAG ATTGTGTTGG TGGGCTCTGA GGAGGGTCTC 
TACGCACTGA ATGTGATTAA GAACTCCCTC ACTCATATCC CGGGTCTGGG ATCCGTGTTT 
CAGATCCACA TTATCAAAGA TCATGACAAG CTGCTGATGA TCGTGGGAGA CGAGCGAGCG 
CTGTGTCTGG TGGAGATTAA GAAGGTGAAG CAGTCTTTAT CCCAGTCTCA CCTCCCCACA 
CTGCCCGAGA TTGTGCCGTA TATCTTTGAG ACCGTCAAGG GCTGTCATCT GTTTGCCTCG 
GGCAGGATAG AAAATGGCCC ATGCATTTGT GCTGCGATGC CCAATAAAGT CACGATTCTA 
CGATACAATG ACAATCTTAA CAAGTTCTGC ATACGGAAGG AGATTGAAAC TCTGGAGCCG 
TGCAGCTGCA TCCACTTCAC CAGCTACAGC ATCATCATTG GTACCAATAA ATTCTACGAG 
ATCGAGATGA AGCAGTGCGT TCTGGACGAG TTCCTGGACA AGAATGACGT GTCGCTGGCG 
TCGGCTGTCT TCACCGGATC CTCCCACAGT TTCCCCATCT CTATCGTCCA GGTGTCCAGC 
ACTCCGCAGA AGGAGGAATA TCTGCTGTGT TTCCACGAGA TCGGAGTTTT TGTGGATGCT 
TACGGTCGAA GAAGTCGCCC TGATGATCTG AAGTGGAGTC GACTTCCCCT GACGTTCGCC 
TTCAGAGAGC CGTATCTCTT TGTGACCTAC TTCAACTCTC TGGATGTTAT TGAGATTCAG 
GGACACGCAG CTCTTGGGCC TCCCAGTCTG GTTCACCTGG ACATTCCTAA CCCACGTTAT 
CTGGGTCCAG CCATATCGTC TGGTGCCATC TATCTGGCCT CGTCCTACCA GAACAAACTG 
CGGGTGATCT GCTGTAAGGG CAGTCTGGTG CGGGAGTCTG GAGAGCTGCA GAGGACCGGC 
TCCAGCAGGA GCCCCAACAA GCGCGGTCCA CCAACATACA ACGAGCACAT CTCCAAACGT 
CTGGCCTCGA GTCCTGGAGC CCAGGAGGGG CCCCTGCACC GCGAGCCCAG CACTCCTCAC 
CGCTACCGCG AGGGCCGCAC CGAGTTCCGG AGAGACAAAT CTCCAGCCCG GCCCCTGGAG 
CGAGAGAAAT CCCCGGGCCG TCCGGTGCTA GACAGCCGCA GAGAGAGGTC TCCCGGTCGC 
TTCGAGAGCA GCGGCAGCGA GAGAGACCGC GAGCGGACAC GACTGCACAC TAGCTCTGTA 
CGCACCCAGC TGACCCCCGT CAACAAGGTT TGGGACCAGT CTTCGGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212894.1
CDS	433..5283
Translation	MEQEISKVQRKMSDLESVLQQKDVELKASETQRNILEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSNKLQEIKEQEYHAQLEEMRVSIRQLEENLSAARRRSDLYESELKESRQTSEELKRKAVEYQQKIQKAKEQGKVEMEELLSKLQKTNGEQQVKIEELQEKLAKAVKASTEATELLQNVRQAKERMERDLERLQTKEDSSDSLRRRLRETEDGKKSLENQVKRLEMVERRESKLKDDIQTKSQQIQQMADKIMELEENLRDSQAMAQRMETQLEQKEKLFEDKIKVLETQMKADLAEKDLLESKQSTFEEESRERGKVISDQAATINAMESKMTSLEQRIAELSEANKLAANSSIYTQKNMKAQEEMISELRQQKFYLESQAGKLEAQNAKLEEHLEKLTQQEQTNKSRVLELEARLREIGLEHEEQKLEIKRQVSELTLSLQEREAQISSLQAARHALDSQLQQAKTELEETTAEAEEEITVLRAHRDEIQRKFDALRDSCAVITDLEEQLTQLTQENAELNRQNFYLSKQLDEATDENEERLQLKQDVERLRREVADREMHLNNQKQNIETLKTTCTMLEEQVMELETLNDELMEKERQWENWRSALDEEKNQAERRARDLQRQLDSEKQNRLRAEQRNSESRQAVELAVKEHKAEILVLQQALKDQKLKAEGLSDTLGDLEKKHGMLEMNARSLQQKLETEREMKQRLMDEQAKLQQQLDMQKSHIFRLTQGLQDALDQTDLLKTERTDLEYQLENIQAAYSHEKVKMEGTITQQTKLIDFLQAKVEHPSKKKKGLFGGRRREDLLAALAAQGQTQVPVVPAATPVPLQYSDLKAALDKERARCSELEDALQKTRLELREVQFNKALEHSSSPATPGLARQQLMMSTKSNKSPEHQSSGGLMSSNSSRGRKDNANPEEYTRRVKDRIHHNTPHRFTQGLNMRAARCTVCLDTVHFGRQAATCIECHALCHPKCSPYLPATCGVPTEYALHLSEALCREKGSTSGLKDMGGHMRLEGWLKQPRNGKRGQGWERKYVVLDGYKVITFETEPRDESVKPLDEFELCLSDGEVTVHSAVGSSELPNTAKTDVPYVLKLESSPQSTCWPGQTLYLMTSGYSEKQRWVAVLEAIVASSRGAREKAEADAKLLGNSLLKLEGDDRLDINCTLPLTDQIVLVGSEEGLYALNVIKNSLTHIPGLGSVFQIHIIKDHDKLLMIVGDERALCLVEIKKVKQSLSQSHLPTLPEIVPYIFETVKGCHLFASGRIENGPCICAAMPNKVTILRYNDNLNKFCIRKEIETLEPCSCIHFTSYSIIIGTNKFYEIEMKQCVLDEFLDKNDVSLASAVFTGSSHSFPISIVQVSSTPQKEEYLLCFHEIGVFVDAYGRRSRPDDLKWSRLPLTFAFREPYLFVTYFNSLDVIEIQGHAALGPPSLVHLDIPNPRYLGPAISSGAIYLASSYQNKLRVICCKGSLVRESGELQRTGSSRSPNKRGPPTYNEHISKRLASSPGAQEGPLHREPSTPHRYREGRTEFRRDKSPARPLEREKSPGRPVLDSRRERSPGRFESSGSERDRERTRLHTSSVRTQLTPVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_017357405.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio citron Rho-interacting kinase-like (LOC100333880), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357405.2

ORF Insert Sequence:

ATGGAGCAGG AGATTTCCAA AGTGCAGCGT AAGATGTCAG ACTTGGAGTC AGTTTTGCAG 
CAGAAAGACG TGGAGCTCAA GGCTTCAGAA ACCCAGAGAA ACATCTTAGA GCAAGACCTG 
GCAACCTATA TCACCGAATG CAGCAGTTTG AAGCGCAGTT TGGAGCAGGC CCGCATGGAG 
GTTTCGCAGG AAGATGATAA AGCTCTTCAG CTGCTGCACG ACATTCGAGA GCAGAGCAAC 
AAACTGCAGG AAATCAAAGA ACAGGAGTAC CACGCTCAGC TGGAGGAGAT GCGAGTGTCT 
ATTCGTCAGC TGGAAGAGAA TCTGTCGGCC GCTCGTCGCC GCAGTGACCT GTACGAGTCT 
GAGCTCAAAG AGTCCCGTCA GACCAGTGAG GAGCTCAAGA GGAAGGCAGT GGAGTATCAG 
CAGAAGATCC AGAAGGCTAA AGAACAAGGG AAAGTTGAGA TGGAGGAGCT TCTGAGCAAA 
CTGCAGAAGA CCAATGGAGA GCAACAAGTA AAGATTGAAG AGCTCCAGGA GAAGCTTGCT 
AAGGCAGTGA AGGCGAGTAC TGAAGCCACT GAACTCCTCC AGAATGTCCG TCAGGCTAAA 
GAGCGAATGG AGCGAGACCT GGAGCGTCTG CAGACCAAAG AGGACTCGTC CGACAGCCTG 
CGCAGACGCT TACGAGAGAC TGAGGATGGC AAAAAATCCC TGGAGAACCA GGTGAAGAGG 
CTGGAGATGG TGGAGCGGAG AGAATCCAAA CTGAAAGACG ACATTCAGAC CAAATCGCAG 
CAGATCCAGC AGATGGCCGA CAAAATCATG GAGCTTGAAG AAAATTTGCG GGACTCGCAG 
GCGATGGCGC AGCGCATGGA AACACAGCTG GAGCAGAAGG AAAAGCTTTT TGAAGACAAG 
ATCAAGGTTT TAGAAACTCA AATGAAGGCT GATTTGGCTG AGAAGGATTT GCTGGAGTCC 
AAACAGAGCA CTTTTGAAGA GGAGTCTCGC GAACGGGGAA AAGTGATCAG CGACCAGGCA 
GCGACGATCA ATGCCATGGA GTCAAAGATG ACCAGTCTGG AGCAGAGGAT CGCTGAGCTG 
TCCGAAGCCA ACAAACTGGC AGCAAACAGC AGCATTTACA CACAGAAGAA CATGAAAGCC 
CAGGAGGAGA TGATTTCTGA ACTCAGACAG CAGAAGTTTT ATTTGGAGTC TCAGGCTGGA 
AAACTGGAGG CGCAGAATGC AAAGCTGGAG GAGCATCTGG AGAAACTCAC CCAACAAGAG 
CAGACAAACA AGAGCCGAGT GCTGGAGCTG GAAGCCAGGC TGCGAGAGAT CGGCCTAGAG 
CATGAGGAAC AGAAGCTGGA GATCAAGCGT CAGGTGTCGG AGCTCACGTT GTCTCTGCAG 
GAGAGAGAAG CTCAGATCAG CAGCCTGCAA GCGGCACGCC ACGCTCTCGA CAGCCAACTA 
CAGCAGGCCA AAACTGAGCT GGAGGAGACC ACCGCCGAGG CCGAAGAGGA GATCACCGTC 
CTGAGGGCCC ATCGGGATGA AATCCAACGC AAGTTTGATG CCCTGCGAGA CAGCTGTGCG 
GTGATCACGG ATCTGGAGGA GCAGTTGACG CAGCTCACGC AAGAAAACGC GGAGCTGAAC 
CGGCAGAACT TCTACCTGTC CAAACAGCTG GACGAGGCCA CAGACGAGAA CGAGGAGCGC 
CTGCAGCTCA AGCAGGACGT GGAGCGCCTC AGGAGAGAGG TGGCAGACCG AGAGATGCAC 
CTCAACAACC AGAAACAGAA CATCGAGACG CTGAAGACGA CATGTACAAT GCTGGAGGAG 
CAGGTTATGG AGCTGGAGAC TCTGAATGAT GAGCTGATGG AGAAGGAGAG GCAGTGGGAG 
AACTGGAGAT CTGCTCTGGA CGAGGAGAAA AACCAGGCCG AGCGCAGAGC CAGAGACCTG 
CAAAGACAGC TGGACAGCGA GAAGCAGAAC CGACTGCGTG CAGAACAGAG AAACTCTGAG 
TCTCGGCAGG CCGTGGAGCT GGCGGTGAAA GAGCACAAAG CTGAAATCCT GGTTCTGCAG 
CAGGCGCTCA AAGACCAGAA ACTCAAGGCT GAGGGCCTTT CTGACACTCT GGGTGATCTG 
GAGAAGAAGC ACGGCATGCT GGAGATGAAC GCTCGCAGCC TGCAGCAGAA GCTGGAGACG 
GAGAGAGAGA TGAAGCAGAG ACTCATGGAC GAGCAAGCTA AACTGCAGCA GCAGCTGGAC 
ATGCAGAAGA GCCACATCTT CAGACTGACT CAAGGACTGC AGGATGCGCT GGACCAGACC 
GACCTGCTGA AGACCGAGCG CACAGACCTG GAGTACCAGC TGGAAAACAT ACAGGCGGCG 
TATTCTCACG AGAAGGTGAA GATGGAGGGG ACGATCACCC AGCAGACCAA GCTCATAGAC 
TTCCTGCAGG CCAAGGTAGA GCACCCCTCT AAGAAGAAAA AGGGGCTGTT TGGTGGCCGG 
CGGCGGGAGG ATCTGCTAGC GGCGCTGGCG GCTCAGGGAC AGACACAGGT GCCTGTGGTG 
CCCGCCGCCA CTCCTGTGCC CCTGCAGTAC AGCGACCTGA AGGCGGCGCT GGATAAAGAG 
CGAGCCCGAT GCTCCGAGCT GGAGGACGCC CTGCAGAAAA CACGCCTGGA GCTCAGAGAA 
GTTCAGTTTA ATAAAGCTCT GGAGCACAGC TCTTCTCCAG CCACTCCAGG TTTGGCCCGT 
CAGCAGCTCA TGATGTCCAC CAAGTCCAAC AAGTCTCCAG AGCACCAGTC CAGCGGCGGC 
CTGATGTCCT CAAACTCCAG CCGCGGACGC AAAGACAACG CCAATCCTGA AGAGTACACC 
AGACGTGTGA AGGACCGGAT TCATCACAAC ACTCCTCACC GCTTCACGCA GGGGCTCAAT 
ATGAGAGCGG CTCGCTGTAC TGTGTGTCTG GACACAGTCC ATTTTGGACG TCAGGCTGCA 
ACATGCATTG AATGTCACGC GTTGTGTCAC CCAAAATGCT CCCCGTACCT CCCTGCCACC 
TGCGGCGTTC CCACTGAATA CGCGCTGCAC CTCTCAGAGG CTCTGTGTCG GGAGAAGGGA 
AGCACTTCCG GCCTGAAAGA TATGGGTGGA CATATGAGGC TTGAAGGATG GCTGAAGCAG 
CCCAGGAACG GGAAGCGCGG GCAGGGCTGG GAGAGGAAGT ATGTGGTGCT GGATGGATAT 
AAAGTGATTA CATTCGAGAC AGAACCAAGA GACGAGTCAG TGAAGCCGCT GGATGAGTTT 
GAGCTGTGTC TCTCTGATGG AGAAGTGACC GTTCATTCTG CTGTGGGTTC CTCTGAGCTC 
CCCAACACCG CTAAAACAGA TGTGCCGTAC GTGCTGAAGC TGGAGTCGTC TCCTCAGTCC 
ACATGCTGGC CCGGTCAGAC TCTGTACCTC ATGACCTCGG GATACTCAGA GAAGCAGCGC 
TGGGTGGCAG TGCTGGAAGC CATCGTGGCC AGCAGTCGCG GAGCCAGAGA GAAAGCTGAG 
GCAGATGCTA AATTATTGGG TAACTCTTTA CTTAAGCTAG AAGGTGACGA CCGTCTGGAT 
ATCAACTGCA CCCTGCCGCT GACCGACCAG ATTGTGTTGG TGGGCTCTGA GGAGGGTCTC 
TACGCACTGA ATGTGATTAA GAACTCCCTC ACTCATATCC CGGGTCTGGG ATCCGTGTTT 
CAGATCCACA TTATCAAAGA TCATGACAAG CTGCTGATGA TCGTGGGAGA CGAGCGAGCG 
CTGTGTCTGG TGGAGATTAA GAAGGTGAAG CAGTCTTTAT CCCAGTCTCA CCTCCCCACA 
CTGCCCGAGA TTGTGCCGTA TATCTTTGAG ACCGTCAAGG GCTGTCATCT GTTTGCCTCG 
GGCAGGATAG AAAATGGCCC ATGCATTTGT GCTGCGATGC CCAATAAAGT CACGATTCTA 
CGATACAATG ACAATCTTAA CAAGTTCTGC ATACGGAAGG AGATTGAAAC TCTGGAGCCG 
TGCAGCTGCA TCCACTTCAC CAGCTACAGC ATCATCATTG GTACCAATAA ATTCTACGAG 
ATCGAGATGA AGCAGTGCGT TCTGGACGAG TTCCTGGACA AGAATGACGT GTCGCTGGCG 
TCGGCTGTCT TCACCGGATC CTCCCACAGT TTCCCCATCT CTATCGTCCA GGTGTCCAGC 
ACTCCGCAGA AGGAGGAATA TCTGCTGTGT TTCCACGAGA TCGGAGTTTT TGTGGATGCT 
TACGGTCGAA GAAGTCGCCC TGATGATCTG AAGTGGAGTC GACTTCCCCT GACGTTCGCC 
TTCAGAGAGC CGTATCTCTT TGTGACCTAC TTCAACTCTC TGGATGTTAT TGAGATTCAG 
GGACACGCAG CTCTTGGGCC TCCCAGTCTG GTTCACCTGG ACATTCCTAA CCCACGTTAT 
CTGGGTCCAG CCATATCGTC TGGTGCCATC TATCTGGCCT CGTCCTACCA GAACAAACTG 
CGGGTGATCT GCTGTAAGGG CAGTCTGGTG CGGGAGTCTG GAGAGCTGCA GAGGACCGGC 
TCCAGCAGGA GCCCCAACAA GCGCGGTCCA CCAACATACA ACGAGCACAT CTCCAAACGT 
CTGGCCTCGA GTCCTGGAGC CCAGGAGGGG CCCCTGCACC GCGAGCCCAG CACTCCTCAC 
CGCTACCGCG AGGGCCGCAC CGAGTTCCGG AGAGACAAAT CTCCAGCCCG GCCCCTGGAG 
CGAGAGAAAT CCCCGGGCCG TCCGGTGCTA GACAGCCGCA GAGAGAGGTC TCCCGGTCGC 
TTCGAGAGCA GCGGCAGCGA GAGAGACCGC GAGCGGACAC GACTGCACAC TAGCTCTGTA 
CGCACCCAGC TGACCCCCGT CAACAAGGTT TGGGACCAGT CTTCGGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

LOC100333880 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

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ORF Insert Sequence:

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